LOCUS       DAOSYL010000001       160078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_48_length_160078_cov_8.195709, whole genome shotgun sequence.
ACCESSION   DAOSYL010000001 DAOSYL010000000
VERSION     DAOSYL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 160078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 160078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..160078
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48_length_160078_cov_8.195709"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..659)
                     /locus_tag="PLU15_00005"
     CDS             complement(150..659)
                     /locus_tag="PLU15_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO29334.1"
                     /translation="MKVKTEQIAVSGVLAALTVVLGATGVGFVPLPTPAGAATLMHIP
                     VILAGILEGSGIGSMVGLIFGLFTLRFMGDWRVVIPARLLIGPVSYWVYVALKKKPWS
                     LPLAAAAGSITNTVGTLALAVTFGYIPLAGKTGAISIAVMQGVPEAIVSALLIPPIVM
                     AVNKAMHRV"
     gene            complement(756..1802)
                     /locus_tag="PLU15_00010"
     CDS             complement(756..1802)
                     /locus_tag="PLU15_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOO29335.1"
                     /translation="MHGITEVLGVLVGHAQDDDALTGCTVVLTPNGAVAGVDVRGSAP
                     GTRETDLMRPCNLVERIQAVVLSGGSAYGLDAASGAMQFLEEEGVGFETPFGVVPIVG
                     AAVIFDLGIGNPKVRPDRSMGYRACRAASSGACAEGNVGAGAGATVGKMLGDGYAMKG
                     GIGTCVAELPQPGRRKPVHVGAIVAVNALGDIVDPWTGNVVAGAYDRAEKRFIGPGAA
                     RRAGRSGRGPMDVYTAKDPEDLAGMNTTVAVVATDAMLDKEGANKVAGMAHDGLARAI
                     VPAHTMYDGDTVFCLSTGKRSLSRDRAADITAIGSLAAEVLAAAVVRAVRASRSVDGY
                     VAACDVAEVASDLD"
     gene            complement(1841..3124)
                     /gene="eno"
                     /locus_tag="PLU15_00015"
     CDS             complement(1841..3124)
                     /gene="eno"
                     /locus_tag="PLU15_00015"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOO29336.1"
                     /translation="MSIITNVYAREILDSRGNPTVEVEVTLFDEVVGRASVPSGASTG
                     QHEALELRDGDRDRYMGRGVTLAVDNVNGPIADEIIGMDAREQAIIDATMIDLDGTPN
                     KENLGANAILGVSLAVAHAAAEFFGLPLYQYLGGVGARTLPVPMMNILNGGEHADNNV
                     DIQEFMIVPVGADSFSEALRMGAEIYHSLRSVLKKRGLSTGVGDEGGFAPNLSSNEEA
                     IAVIVEAIEKAGYGPGEQVYIALDVAASELYRDGKYRLEGEGVEYNSEQLVSYYQALA
                     DKYPIISIEDGLSEDDWDGWKIATERLGGKIQLVGDDLLVTNVERIEKAIERHVANSV
                     LIKVNQIGSLTETLDAIEMAKRAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGAP
                     CRTDRVAKYNQLLRIEDQLGDAAVYAGLDAFYCIR"
     gene            complement(3170..3940)
                     /gene="tpiA"
                     /locus_tag="PLU15_00020"
     CDS             complement(3170..3940)
                     /gene="tpiA"
                     /locus_tag="PLU15_00020"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO29337.1"
                     /translation="MRRSIIAGNWKMNKTIPDGVALGGALADDAARFIEKCDVVLCPP
                     FTAIKAVADRVAESGIDVGAQDVHWETSGAFTGEISCEMLLDAGCRWVIIGHSERRAM
                     FGETDDTVNRKMKAAVAAGLKPIVCVGETLAQREDGLANDVVRRQVRGAFAGLSAEDA
                     SKAVVAYEPVWAIGTGRASTGEDANAMARVIRDTIAELYSRDLSEAVRIQYGGSVKPG
                     NMAEFARQPDIDGALVGGASLKAADFMGIIAAMIDSKE"
     gene            complement(3937..5142)
                     /locus_tag="PLU15_00025"
     CDS             complement(3937..5142)
                     /locus_tag="PLU15_00025"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO29338.1"
                     /translation="MRKLTVEDINVAGKRVFVRVDFNVPMDAQRNITDDRRIAAALPT
                     IEYLLDHGAKVILASHLGRPKGKYDPSATMDPIAERLSELLGKPVRKLGDCIGPEVVQ
                     AVGEMEPGDVVLLENLRFHREEEANDPRFAEALASLADVYVNDAFGTAHRAHASTEGI
                     AHYIPGVAGFLMKKEIEIMGKAIEDPVRPFIAVLGGAKVADKLGVIDNLLDKVDGLIV
                     GGGMAYTFLAAKGYEIGKSILDAERLDYARGAMAKAKDRGVTLELPVDIVAAERFDAD
                     SPYKIVDADGIPADWMGLDIGPRTVERFSNLIRSAGTVIWNGPMGVFEMPAFSRGTRG
                     IAEALASSDAVTIVGGGDSAAACEQYGLADKMTHVSTGGGASLEFLEGRELPGVAALR
                     DEGDMEGLK"
     gene            complement(5172..6176)
                     /gene="gap"
                     /locus_tag="PLU15_00030"
     CDS             complement(5172..6176)
                     /gene="gap"
                     /locus_tag="PLU15_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOO29339.1"
                     /translation="MAVKVGINGFGRIGRLVLRAADGHDDIEVVAVNDLMDARTNAHL
                     FKYDSVHGTYPGKVVADDDCIEIGEKKIKVFAEKDPAKLPWGDLGVDIVVESTGVFRS
                     KDKASAHLAGGAKKVIISAPAKNEDITIVMGVNEDKYDHANHHIISNASCTTNCLAPV
                     AKVLMDTFGIKRGLMTTVHSYTNDQRILDLAHKDLRRARAAGMSMIPTTTGAAAAVAL
                     VLPELKGKLNGFALRVPTPNVSVVDLVVDLEKKATVDDVNDALRAAAEGPLAGILDYT
                     EEELVSHDFNHNPHSSIVDGLCTMVMEDDMVKVIAWYDNEWGYSNRVVDLASFIAKQM
                     "
     gene            complement(6281..7342)
                     /locus_tag="PLU15_00035"
     CDS             complement(6281..7342)
                     /locus_tag="PLU15_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOO29340.1"
                     /translation="MSSEMRMLSAAEIVSLQRRVMPEFSDRIILRYNILRHVSHAQPI
                     GRRALAQSLGESERHVRREENVLRTAGLLVVGPDGIRLSEDGEDILRDLAEYVGLLVG
                     AQRLADLLQHEFDLERAIVITGNSDEDEAVKKEMARVGACVLRDMISDGDVVAVCGGT
                     TMAELAAQTVPASGKRNVVVVPARGSLGEDVEKQADTVAALMAKKLGGVYRILNLPDD
                     LGHELAASVAEEPRTKEVLDTIRQSRIVVHGVGTAVEMARRRNMSPSEIEEIASLGAV
                     GEAFGFYFDRDGEVVYITSSLGLRVSDLSAVETVMCVGGGSSKGQALAAVLRNGFSHI
                     VVTDEGAAREALGKTVDDA"
     gene            complement(7436..8863)
                     /gene="rpoN"
                     /locus_tag="PLU15_00040"
     CDS             complement(7436..8863)
                     /gene="rpoN"
                     /locus_tag="PLU15_00040"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOO29341.1"
                     /translation="MSFVLVQQQVQKLVMTPELRQAIHLLQLSTVDLAEYVSNELLEN
                     PLLEEEGHVGMVVDKQVQAGPENAVHESDANPEDDENEIDWERFFEDGTDVGWTRGPI
                     DPNVDFDPIPLVKWEPSLEEQYHMELRLACSDMRTLRIGEYLLGCIDGDGFFRGDVHR
                     VAASLGVEIDDVERALSLIQSFEPVGVGSRSIRECLDLQICASGVEDNLCRLARKIVS
                     CHLEDIAAGRMVKVSSELGVDVRQVQAAVDFIRTLNPRPGAGFTGSGETRYIVPDVFI
                     EYVEGEYIVIPNDSAIPRLMINEKYRSMLRDANVDSAAKDFIKEKLDSALWLVRSVEQ
                     RRSTIVKVSESIVRHQRDFLDRGILHMRPLTLREVADDIGMHESTVSRATAGKYAQTP
                     RGVFELRFFFDSGVSTSDGDAAASASVKKRIGEMIASEDPKAPFSDQQIADALGCEGI
                     MISRRTVAKYRDEMMLPPSTKRRRY"
     gene            complement(8881..9822)
                     /gene="whiA"
                     /locus_tag="PLU15_00045"
     CDS             complement(8881..9822)
                     /gene="whiA"
                     /locus_tag="PLU15_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOO29342.1"
                     /translation="MDFYSRMKDELIRVMPTQRCCQVAELSALVKTDGVIQISQGRIS
                     VLIRESNAGVARKVVMLLRDVGRPDTQIAVRQGSGLPRGNTYIVLISDHDQAMELLEL
                     LGIYGEFGLASGIDSAIVANRCCMRAYLRGTFLGAGYMGEPEKGYHVEMALKYRTHAD
                     DIVELLRQFHIRAGIVQRRERSVVYIKHGDDVVEFLRVIEAANALLELENTRIVRSIR
                     NDVNRLVNAENANVSKVVEASVRQIQDIQLIDEMMGIGRLPPALREIAWLRLEYPEAS
                     LTELGTMLSPKLSKSGVNHRMRRISEIANRLRGQQES"
     gene            10012..10629
                     /locus_tag="PLU15_00050"
     CDS             10012..10629
                     /locus_tag="PLU15_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOO29343.1"
                     /translation="MSIMWLLMLASGAIVAIIVKGPASVTSIALSSTREAVQLCIGLA
                     GSIALWSGIAKIAEESGLMSRLADLMQPIIAPLFPGISPRSHAIGYMATSMAANLLGL
                     GSAATPFGLRAMEELAAEDKRHSGKASLRLPAQHQEATDAMCTFVIITASSLTLVPTT
                     VLSLRAELGSADPGSIIGATIIATSCSTGAAIILDRLLSRRRRRR"
     gene            10626..11156
                     /locus_tag="PLU15_00055"
     CDS             10626..11156
                     /locus_tag="PLU15_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOO29344.1"
                     /translation="MMQLSSLPYLLIPLFLLGIPAYGASKGIDVYACFIEGASDGLKV
                     AAKIAPAMIAMFLVVGLLRDSGALDILAQAVGPATMKLGINPDLVMLAILRPISGSGA
                     LSLLANILKDLGPDTFDGKLASVIQGASDTSLYILALYFSSVNVSKWRHSAVAALLAN
                     AVGFVAAIIACNIIFR"
     gene            complement(11143..12138)
                     /locus_tag="PLU15_00060"
     CDS             complement(11143..12138)
                     /locus_tag="PLU15_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOO29345.1"
                     /translation="MRHSPVPKPLSRRLRVVALGGGTGLSTLLRGLKEYTNKITAVVA
                     VTDDGGSSGRLRQEMGMLPPGDIRDCLVALADAEPLMKELFDYRFNGQGPLAGHNFGN
                     LFIAAMSDITGDFELAIKESSKVLVVRGCVLPCTLDNCVLGANLEDGNVVMGETSISS
                     SRRAIRSVFLEPATCSPLPEILEAIEAADVIVLGPGSLYTSVMPNLLVSGVADAIASS
                     NAVKVYVCNVMTQPGETQGYTAQDHLAAIYAHAGADIVDWVIVNNSPVSDRVLENYCR
                     EGSIPVAWDCKALESMGVSVVEAPVINQTNVVRHDPEALSKEIMALAVRHVFTGR"
     gene            complement(12150..13061)
                     /gene="rapZ"
                     /locus_tag="PLU15_00065"
     CDS             complement(12150..13061)
                     /gene="rapZ"
                     /locus_tag="PLU15_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOO29346.1"
                     /translation="MAETTISGQELSCIVITGLSGAGKSEAARAFEDLGYFCIDNLPV
                     MLIPKLAELLSETSGRIDKVALVIDVRGQDVFGQLSSVFRTMDDSGFNYKVLFLEASD
                     ETLVNRFKASRRPHPLAPEGGILDGLKLERQLLARLRAGAALVIDTSDLTARQLRAEI
                     VRRFGDNSGAPTIQLTVVSFGFKYGVPADADLVFDVRFLPNPFYVDSLRNLTGNDEEV
                     NEYVMRWPVTKRFLDKLVDLFRFLLPEYEREGKFNLVVGIGCTGGRHRSVVIANQLSR
                     PLRELGYRTHTMHRDIDIGSGDQPASK"
     gene            complement(13309..14907)
                     /locus_tag="PLU15_00070"
     CDS             complement(13309..14907)
                     /locus_tag="PLU15_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOO29347.1"
                     /translation="MDHIGTFSLVLHSHIPYCRRSGVWPFGEEWVFEAVVETYIPILD
                     WLLNCHEKGVGPNVTLSFSPVLAEQLADAYMKSRIVEYMDARANAARADLMRHELVHD
                     DRLARLSRKYVEYYENAKKRFIDYYDGDLLGAIAKLAASGRVEVLATSASHAYLPLLP
                     QESRIRTQIRVGYEACQRHFGVLPKGAWLPECGYKPGAIERALEECGIEYFIVDTPAI
                     AGGGPRNLYGGVPEALRRNIGAGLPSMATLMPYRLRGSKMAVFGRDEVTAAQVWSRDV
                     GYPGDGNYLEFHKRDDSSGLRYWRVTNKLLDFGQKQLYDPEAALASARTHAQHFAGVV
                     EERLGGYAERTGRHGIIVAPYDMELFGHWWHEGVDWLSLAMDALCASNRVECGTLGSY
                     LKLHPPVDELELPECSWGHGGRHGVWFNSETEWMWQEIERATAIGRELEGVIGGLDGV
                     HKIMADQALRELMLLEASDWPFLVTTGQAGGYARDRFAQHLSDFEVLAQAAMARETDL
                     RSLEVLEASSSRDSVFTWIDWGSL"
     gene            15074..16096
                     /locus_tag="PLU15_00075"
     CDS             15074..16096
                     /locus_tag="PLU15_00075"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOO29348.1"
                     /translation="MQDSFDKVSPIDKKGAGTPSVSQHEQQMAEDARIELSPPEVSDQ
                     PLPHRRFPEITILETENLQMNPARRHKPTVLPGFYGATRIVLQVRDPFWMHAYWEIAE
                     DMAKNLRRQLGEELDRSQLTLRVHDVTGVTFDGHNSVRSFDKEVHPFANNWYINVEES
                     DRSYCVDLGLVTGASQFYLISRSNTVHTPRIGPSWIVDEEWLTIREIEEGYISHGLSP
                     SSPGISRHARAEELLHEIALGSGGVGAVGSPMGQPGEVHRAPRGFWLRADAELIIHGA
                     TEAGAKVEVCGMPMPVRPDGSFSVRLSLPFGDHPVDISATSDDGIMHRSVSWIVSRSN
                     RGNGRS"
     gene            16100..17704
                     /locus_tag="PLU15_00080"
     CDS             16100..17704
                     /locus_tag="PLU15_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOO29349.1"
                     /translation="MSTPNGYLALVLHAHLPFVRHADGEHYLEEKWFYEALIETYIPL
                     YIVLSRLADEEVPYRITISVSPTLLSMFRDPLLQQRFSDYMNNLVELAEREVARTRWL
                     PAFHDTAQMYRDRFREARDVYWNRLGGDMAKGLQALSDSGHVELITTAATHGYLPLME
                     VCPEAARAQILAGIDYYRQVFGIAPKGFWLPECGYSPGIDEYLREAGVRYFFIDSHGI
                     LNASPRPRYGVYAPVYCHSKVAAFGRDMESSKQVWSAQEGYPGDYDYREFYRDIGYEL
                     DWESIKPLMGNCPIRLQTGLKYYRVTGNTDRKEPYVRQWAIEKAATHAGNFVFNRERQ
                     VEYLASVMDRKPIVVAPYDAELFGHWWFEGPEFLDYVIRKIAYDQNTIALLTPSDYLE
                     LYPKNQVATPSMSSWGYMGYSEVWLADCNNWIYPHLHTMAGRMTELARSYPHPQPELT
                     RRALAQAARELLLAQSSDWAFIMKAGTCIDYAKKRFETHTAHFLGLYDDIKSSNIDPE
                     WLSELEEQSNLFPNIDYMVYAGRATM"
     gene            complement(17878..20334)
                     /locus_tag="PLU15_00085"
     CDS             complement(17878..20334)
                     /locus_tag="PLU15_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO29350.1"
                     /translation="MKAVIMAGGKGTRLRPLTCSMPKPMVPVVNRPMMEHVVDLLVAH
                     DFRDLACTLCYMPEAIQEHFGDGSSVGCTMTYSVENTPLGTAGSVRALGDFLDDTFLV
                     ISGDALTDIDLTSAVTFHRERGAAVTIVLTRVATPLEYGVVIVDGDARITRFLEKPSW
                     GEVFSDTVNTGIYVLEPEIMQRVDPSCEFDFSKNLFPLLLAEGYPMYGYIADGYWCDV
                     GNLDQYRKTSQDILEGKVRVKIPGDLVDEGIWVSEGAEIGNIAALRPPVVIGAGAKIE
                     AGAAVGEYSVVGPSCIITEGASVRRSILWTGCFIGQNAEVHGAILGSRVSAKAGVLIQ
                     EGAVIGSGCSMGERAQVRPGVKIWPDKTLDGGAQISASLVWGASWSKRLFGRLGVTGL
                     ANIEITPDFAARLGAAYGSCLSSGVVAVSCDAHPASDMIKRALASGILSSGISVADLG
                     SGSTAVTRYAVPAIGASGGVHVRVSPTGGNASLIEFLDASGINIDKATERKVENAFLA
                     EDFKRANSDKVGSTITTEGMVGRYLDAISSSIDADTVRRRGLKVVGGYDSEYVGALID
                     AFCNACGCDLHHVEISNTAWAPSEVADAVAVTEADLGFLLDRNAERLALVDETGSNIS
                     NEEMLALICVALLTSGRETTMAVPVTASSAIERLAAAFRGYVVRTQANPRAMMEKAIE
                     ARVRMGEASLPHFQPVMDGVFALAVILEYLAREKTTLSAFAQIAPRSYMTKQVVDCPW
                     EDKGRVMRRLIEETDGEEVQLIDGIKVYSDHGWTLILPDSEEPLFHVYSEAHSQEIAE
                     EIAGIYVSRIAETISDAGAV"
     gene            complement(20331..21527)
                     /locus_tag="PLU15_00090"
     CDS             complement(20331..21527)
                     /locus_tag="PLU15_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO29351.1"
                     /translation="MRILMFSWEYPPRVIGGLARHVCELARAMARCAEVHVITCSAEG
                     AQSMDRDRGVWVHRVDPVGPCTDDFVTWAMQLSYGMLARAACLVSEGGAFDIVHAHDW
                     MTSFAGVGMKRAYATPLVATIHATEWGRNGGLHNATQRRISDIEWFLCYEAWRVIACS
                     RHMQAELAGIFGIPADKLRIIPNGVYSEQFQDADADIARRMYGLSEEDSVILFVGRLV
                     NEKGVQVLIEAMPKILSYQPTAVLVIAGQGPCEGDLRELAANMGLGGKVKMVGFVSDE
                     ARNALYRLARVAVVPSLYEPFGITALEAMAAKTPLVSSDIGGLSEIVRHGENGWKCYA
                     GNANSLADAILHVLYSPELARQMAETAYRDVQTIYDWRAIADRTLRVYEEVLREARHA
                     EEAMVR"
     gene            complement(22006..24537)
                     /locus_tag="PLU15_00095"
     CDS             complement(22006..24537)
                     /locus_tag="PLU15_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29352.1"
                     /translation="MYAKSTSRICAFAIALFVLMFGVPAQSSLGWPIWEWDWDTEPAE
                     PGEPHVPAEAMLSEEDPSEDDTERILDVVFAFGLMGNVSPDSAGQASATCERLLDVLL
                     SYPDMRFSVGISGTLLTGLAWEGSRAVQLLRDGIGRGVIEPLGATYSEGVLASMEPWD
                     AQLAVSLGSEAVSSFLGEKPTGFWNPQGVWRQDVIVPVAIGGYTHTILEDTIVEASGL
                     GMLPPNAPMRVSWGGREAVLFQADTDFGRLVDNAIRTGSTRPVIDYLQQLYEGDKQDS
                     AVVVYAKELMVTEDKTEWEGLSLLLRALADEEWIRVTTLRERVEAGPPPGSVIQIADG
                     VPRGFQKALSDEGFSDWAAFNSSRNLEALRVLYSDIRARIHSVEAAVRKAEASGKDTS
                     SAQRLLDDAKMVFISSQYGFGRPGSAGAVSGDANWEMGRAAYVSALAAWQCVNPTVSA
                     YQEDINRDGVEEVIMITDTDMFVLSPIGAKLLYWYDLENGEELVGSAVQQYLGEIFVN
                     ESMAGGGRQRALVDSISGPAAVGIDLSSAEFGVEIGEDSPSATFVFKDGGTAIEKSVA
                     AKEGALEVDYTVRSRGRISFAVSNGLCPDAHWTRIGGKDSLVYYDPNGAVASSLRQGK
                     SFGLMNVVTGTVVRLTVGGDRDDGVPLSRVSSTLDSFVRRLDLEYKFAMAPGASANFG
                     FELQRSKAWPSPSAVLWIQEVDNEIVVGLPTYTRACAIRQFVDGYVTDTPMVAFSSTL
                     KKARKGQEKSALEVGAPLPKGRYDLVAVLVTDRKALIENAGSYVSGTGKHEFFPEVRD
                     SVVEITPGYARVGGTVVMWVAIGVGAGVVVAGVIVYLRRRKVSGA"
     gene            complement(24552..25481)
                     /locus_tag="PLU15_00100"
     CDS             complement(24552..25481)
                     /locus_tag="PLU15_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOO29353.1"
                     /translation="MKRLCLALIIVLLPALAVMMWSARTSAAHFPAGVGQRLIWEADD
                     PEGDDYGPGTYVYPTNPAFAPFSGLFDLIGFRVSCDDENVYFDTKLAKISNTWNAPEG
                     FSHQLINIYIDTTSGAGRTDTLREGAFVSFAPEYAWDVNIKIMGWGGSRVFFASDNPA
                     SAGIIEGLSVEALPDGNTIRATVSRQVIGEPLDSWKYYVLVSSQDGYGLDNFRPVMGE
                     AGPWVFGGGSDLNINPNVIDMLAPESGVYSQESQLGSWDVSERRYARIVPANAQLHRR
                     HLTVRTVILIIAVGGAAALAYIYFRGRSNVGRG"
     gene            complement(25521..26513)
                     /locus_tag="PLU15_00105"
     CDS             complement(25521..26513)
                     /locus_tag="PLU15_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO29354.1"
                     /translation="MKNKIEHGKRHFIGVDLGGTKIASVLVNEDNLPLANVQMPTCPE
                     EGQDAVMERIIASISELASKLPGGIDDVEAIGIGSPGPLDKELGMVVDAPNLKWKDVP
                     VTSTIHVATGLPVYLENDANVAALAENRMGVGRGTKNMVYITVSTGIGSGLILDGRLY
                     SGTTGGAGELGHVTVVPDGPMCGCGNRGCLESLASGTAIAKRAREAATRTAGSGILSA
                     AGGKIDNIDAVLVSSCAEAGDPMATAILRNAFEYLGIVVANMVNLLNVEMVVIGGGVS
                     QIGGMLFDTVRRVVDLRAFPFMAKDAKIVPAALGVDAGMLGAACYAMEKQSGED"
     gene            complement(26595..27701)
                     /gene="ugpC"
                     /locus_tag="PLU15_00110"
     CDS             complement(26595..27701)
                     /gene="ugpC"
                     /locus_tag="PLU15_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOO29355.1"
                     /translation="MAGVTLEHVTKKFGNVVAVNDADLEIKDKEFLVLVGPSGCGKST
                     TLRMVAGLEEITSGNIYIGDTLVNDIPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKFPKEQIDERVKDAARILGIENLLDRKPKALSGGQRQRVAVGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRSELSKLHNRLQTTIIYVTHDQTEAMTMGDRIVVMKDGFIKQVGAP
                     LEIYQYPDNMFVAGFIGSPAMNFIPGVLRKKGDGYIMDCESFQITVPGNRFKDLGKYI
                     DERMVLGIRPENIQDADFVEDAPADRTVKCMVDVVEPMGAEAYLYLTVGPHSFVARVN
                     PRTTARDGEPHAVVLNMEECHLFEASTENAIAIK"
     gene            27846..29084
                     /locus_tag="PLU15_00115"
     CDS             27846..29084
                     /locus_tag="PLU15_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO29356.1"
                     /translation="MDERKPLVLMRGMIAIPVGNLQEQLAFKGFPLGEVDNVFGIRTE
                     LAVKQFQASVGLPETGIVDAQTWRLLFDGLPLPDEVFCPDDSLRYDNTVDYRGYSDRS
                     SAGRMCISADKDKSTNGGSELALSASSKSRQGPSIHVSLSQRRLTLLQSERESAQYPV
                     AIGKPATPTPVGNWSILNKALNPGGVFGTRWMGFTRQGHGIHGTNQPQSIGHAVSNGC
                     IRMYTPDVESLFTQVSVGTPVVIDAGVPFPTPAPGTYIVRSGDTLYLIARRFGTTVEA
                     IRRTNNLTTDIISVGQVLFLPGPTGAPPIIGTDYVVQPGDTLFLIARRFGTTVQAIMS
                     ANNLTSTNIYVGQRLRIPGGPRTAAQMRAQSGASEYVVQPGDTLHSISAAFGIPVEAI
                     IAANTMISSDIYPGQLLRMP"
     gene            complement(29565..30887)
                     /locus_tag="PLU15_00120"
     CDS             complement(29565..30887)
                     /locus_tag="PLU15_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29357.1"
                     /translation="MKKLAVILAAAMIATVALPVAAANVSVGGKLDSKMMYDFGADTP
                     SAETNLKLNLNVGPIGGQTITGVIEFNPWLIAEKNGCGMAPKPVENGDPVPGAIRRAY
                     LEAVGPYWTDGPAVTSRLGDLELAYSPFILSTEALNGMSVSGIAYGPVEAGVFHGFDK
                     GIRARGARVAAGVANVAVDASAVKVGDDELDYAAYAIATPIPGLALDATYAAQTNTKA
                     AAIRANAAAKVPYLPLDVIATAGYRSTDLAFNPTYRWMEEGNPVDGEQGKAAVTAGLA
                     TSYAGFDVALNGEILGDDDDGKLDDGRSIGATVGTAISGFDLTAGHKVTQTMGEKIET
                     HNTTTLGIAMKEREVLPNMLISASYNAAMPDFSLAETRHIAKASVDADVSVLKGIKVS
                     ALYDTKPATGKPGRELGIEYGAPCGVKLGYSCNDLNTNMVYAGMEVEF"
     gene            complement(31992..32960)
                     /gene="argF"
                     /locus_tag="PLU15_00125"
     CDS             complement(31992..32960)
                     /gene="argF"
                     /locus_tag="PLU15_00125"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO29358.1"
                     /translation="MTKAHQLLYDELGRSFSGKDFLSIHDFTSEQIMRVMELSALLKA
                     EQKAGVRHHLLEGKTLGMIFLKPSTRTRVSFETAIWQLGGYGLFLSSNDLQIKRGEPV
                     PDTARVLSRYLDAIMIRTFGHDEVVELAEWASIPVINGLTDLLHPCQVMADVFTGIER
                     FGDLRGRKLTYIGDGNNMVHSLMYAGAKLGMHVTCACPDQYQPDAAVMEAAKADAVAT
                     GARLEVVRDPKEAARGADILYTDVWASMGMEAEQEKRKKDFQGYCIDQEIVDAANPDC
                     IVLHCLPAHRGEEITHEVMEGSHSAIWDEAENRLHVQKAILALVMS"
     gene            complement(32974..33981)
                     /gene="amrS"
                     /locus_tag="PLU15_00130"
     CDS             complement(32974..33981)
                     /gene="amrS"
                     /locus_tag="PLU15_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOO29359.1"
                     /translation="MHARVEDSLGICELCPRRCKLQEGATGACRVRTMIGGRIIPKNY
                     GAITSMAIDPIEKKPLYHFMPGSGVLSVGTFGCNLSCRYCQNWEISQRHAESRFFTPD
                     QLADLAESMREQSVGVAYTYSEPFVWYEFVYDCAKLVHEIGLSNILVTNGYINPEPLA
                     GILPYVDAINVDLKGYDDEYYRKVCGGTLAPVISTIRAVYESGCHLELTTLVVPGYND
                     SPEEMEQLASWVASLSPEIPLHISRYFPSYRMNEPPTPMATMEKCVEVAKRRLNYVYV
                     GNASIPGGSDTVCPNCGELLIERRGFACVTVRLEGVSCPKCGHKIPVRLAGGESAGNK
                     Y"
     gene            complement(33989..35392)
                     /gene="amrA"
                     /locus_tag="PLU15_00135"
     CDS             complement(33989..35392)
                     /gene="amrA"
                     /locus_tag="PLU15_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOO29360.1"
                     /translation="MGELVLAALAPHPPIIVPGVGGREAAKAQKTIDAMEELAREFAA
                     ARPDTVVVISPHAPVFADAIAIDGRPHLSGDLAQFGADVELDFYSNLELMQAIEDEAH
                     AAGISVAVLEPALARRYRVPSGLDHGAVVPLYFLAKEHYPFRLVAVSMGVGALNELYT
                     FGMCMASAIDKSQYRVAVIASGDLSHRLSPDAPAGYAPEGKIFDEAVVGALGTMDVAA
                     LARLDDALIEAAGMCGLNPIIMMLGSLDGIEVESEVLSYEGPFGVGYAVVALRPKGRS
                     EEARRFHELVEGGKEKTRKLRHSESAFAALARRAVEDYVRRGVVVEPPDPLPEGMQGR
                     AGVFCTLHKRGQLRGCIGTTEPTRRNIAEEIIFNAIAAAVRDPRFEPVAPHELDDIVY
                     SVDVLSPPEPVSGPEELDPQRYGVIVRNGGRVGLLLPNLEGINTVEEQISIARRKAGI
                     GLNAPVELERFEVTRYE"
     gene            complement(35380..36678)
                     /gene="murA"
                     /locus_tag="PLU15_00140"
     CDS             complement(35380..36678)
                     /gene="murA"
                     /locus_tag="PLU15_00140"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO29361.1"
                     /translation="MELVIAGGKRLVGSVRAGGSKNAAIAIMAGSLLAEGDTIIENAP
                     GARDVSAMSAVLREMGVSIRRAGPSALRVRTHRLIHHEPPYELAKTLRASSLLLGPLL
                     ARKGKASIPLPGGDSIGSRPMDLHIKGLRALGADIEVEHGYIVAKAPRLEGARVYLDF
                     PSVGATENIMMAACLANGTSIIENAAKEPEIVDLANFLTLMGANIRGAGTDTIRVDGV
                     DSLRGVAYSVIPDRIEAATLLAAGIITHGQVKVGSIIPTHLESVLSKFSEMGASITVG
                     DDYVEAAMDGPIQPCDTKTLPYPGFPTDMQPQLSAVLSVASGVSVITESVFESRFFHV
                     DELKRMGADIRLEGRSIIVEGVPGLTGARVHAGDLRTGAALILAGLAAEGETVISGAE
                     NIDRGHDDLCSKMNSLGARVKRQDSQRCVAAGEENETWVN"
     gene            complement(36778..38034)
                     /locus_tag="PLU15_00145"
     CDS             complement(36778..38034)
                     /locus_tag="PLU15_00145"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOO29362.1"
                     /translation="MKRPVIFIALPVRTAVLIGIAVLIGVVIYAVQVLVVTTLAPVRD
                     VVSGKTVAIDAGHGGPDSGARGKSGIIEKDINLHIALELRQLLGRVAVYTVMTRESDH
                     GLIADGEDITGSRKRMELERRANLVNANSPDIFVTIHTNSFPEPQWSGAQVFYNAASA
                     EGRKLAVHIQTELVNRLGPNTRKARPADIYLLKKVKAPSALVEVGFLSNPREERLLAD
                     AAYRVKVAEAVFHGIMAYFMDSQGEAGNVLAPDDDSDEAWGLVTKVGTATSQLTPDST
                     ILYFGGPTNLDDSLMPEAREIPGLKSLNKIEALAAVVRATIAGPGKDSILQPTIPRGT
                     VLRSVRLQGDTAVADFSAELVMNHWGGSRSEEMTVYSIVNALTEFSYVKKVEILVEGK
                     PLQTISGHMQIEGPLERNLSIVHFRT"
     gene            complement(38205..38816)
                     /locus_tag="PLU15_00150"
     CDS             complement(38205..38816)
                     /locus_tag="PLU15_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO29363.1"
                     /translation="MCALSEHYFTTSPTSPHERRIIPYTPRGGRELSFETDAGVFSKT
                     RVDRGTDQLIQAIRISPGQSLLDLGCGYGVVGISIASLHPECEIWMTDINERACELAR
                     ANARRNGVERVTVVSGDGFDPVRGRMFHVIATNPPIRAGRAVLYRLIEEAHDHLEPGG
                     SFYAVVRTKQGAASLKAFIAQTYGNADEPEIGGGYRVIRAFRE"
     gene            complement(38920..40332)
                     /gene="ypeB"
                     /locus_tag="PLU15_00155"
     CDS             complement(38920..40332)
                     /gene="ypeB"
                     /locus_tag="PLU15_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HOO29364.1"
                     /translation="MTDGTSGARGKMRNWPQALLLVIVIVSLVFAYSQWAAKQRLEVA
                     VGNQYFRSFFDLLGNVDNVRVALSKSLASGSPRLRVGALSDVWREASNAQANLNNLPV
                     THKVLARTSAFLTQVGDFAFTTARKVANQQVMSEDDWNKLVDMKKQSEDLARGLADMQ
                     AQVSTGKVNWVRVAEQAKSEVKPKPGTSATPDVVKDGFTRVDEQVQTFPTLIYDGPFS
                     DHIEQIRPRGITGAEITADQAKEVARRFVPFDASSHSATVEENIKAKGDIDIYRVRLD
                     APKRSGKPDAVVDVTKKGGHVVLMNVARDLDKKTIDLAESVKRASAFLSKVGMVNMTP
                     TFASEVGDVSIVPFVYTQDGVVIYPDQVKVKVALDNGDILSYDALAYLTSHTMRRLPK
                     PSIDADEARELLSPALTVTEPARLAVIPVETVDTPEAFCWEFRGTSYGEQFFVYINAE
                     KGTEEKVLQLITTSEGSLTM"
     gene            complement(40349..40798)
                     /locus_tag="PLU15_00160"
     CDS             complement(40349..40798)
                     /locus_tag="PLU15_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOO29365.1"
                     /translation="MRMPNARILVFVVLAVAVVIGTVAHIGRAQSRSGDVNLLARLIA
                     AEAEGEPYQGMVAVGAVMLNRVASPKFPNSISGCVFQPGAFESVSNGLIWRRSPSRQA
                     ISAARDAMNGYDPTYGCVFFWNPAKPVSGWIWSRSIAVKIGKHVFAR"
     gene            complement(40836..41546)
                     /gene="sleB"
                     /locus_tag="PLU15_00165"
     CDS             complement(40836..41546)
                     /gene="sleB"
                     /locus_tag="PLU15_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HOO29366.1"
                     /translation="MNLKTPIMGLTVGAMIFIVVLLCAATPAKAETRPTLYWGSRGRS
                     VRLVQWKLSDWGYYRGWVDGVFGANTHRAVLAFQRKNGLKVDGLVGPQTWRALGFGYT
                     VAAEAPAAYKPAPMASRSASVDLVARAIAAEARGEPYRGQVGVGAVLMNRLKNPKFPH
                     SLSAVIFQPNALESVSNGLYWRRTPDQIAYSAARDAINGFDPTYGSLFFWNPAKPVSG
                     WIWTRNIIVKIGKHVFAR"
     gene            41852..42850
                     /locus_tag="PLU15_00170"
     CDS             41852..42850
                     /locus_tag="PLU15_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOO29367.1"
                     /translation="MMHTIGVVFNLKKDFPGLVGKANSVPDLAAECEEEETVDMLSSS
                     LAKIGEKVMLFPHKSDLLERLDEARPDIVFNIAEGWAGRNRESLIPALLELLEIPYTG
                     SDALTLGIALDKAMAKRVASTVGVRTPRYIKVSSIEDLSSMNMDFPVFVKPNYEGSSK
                     GIRNSSRVRNMDELTEKVRWILETYSQPAIVEEFLPGREFTVGIIGNGRPHVLPIMEV
                     CPGNRDSADCEFVYSYETKSGNLERFRVPAPIPEHLASEIADMAVSVFEVMECRDVAR
                     IDFRLDAEERPHFLEINPLPGLSKVSLLPLQARAAGMTYDDLVKWILTSAWSRYWH"
     gene            43041..43292
                     /locus_tag="PLU15_00175"
     CDS             43041..43292
                     /locus_tag="PLU15_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOO29368.1"
                     /translation="MDNRVRCSVNNCHYWNEGNYCHASQIMVTSDSIANQLPDSYDAL
                     QASIAEPRPAAHIGETCCKTFAPKGTSDTTVRSDRTTRM"
     gene            44776..46527
                     /locus_tag="PLU15_00180"
     CDS             44776..46527
                     /locus_tag="PLU15_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29369.1"
                     /translation="MKRTEHLKDFFAKYWWRYVLGILTLIIVDALQLVVPKILGNVTD
                     ALREGSLTPEGLKKHVLIILAIACGIAIGRFLWRIFIMGTARLLDFELRNKLFAHLQK
                     LPARYYTEHKTGELMAHATNDIPSVRQAFGQGFILITDSIFMTTATLIILFRTVPIRL
                     AIFALLPFPLLAVSTAAFSKIINPRHLAVHEAFGELSDRAQESIAGIRVTKSFVQEDA
                     EIAKFMAVAQKNVDANIDLVKIWGLMSPLSGLATALAYAVVLRYGSSMIMYQQISLGD
                     FVAFTSYLAMLVWPMIALGWVINVMERGFTALDRLNAVLNEVPEVADEPGAIELEDVT
                     GTIEFRNLTFSYRDGVPPALSDISFKIDAGKTLAVVGRTGSGKTTIVNLLTRLYNPPE
                     GSVFIDGHDIRNVTLSSLRNQIGCVPQDTFLFSCTIGENIAFADRDFSPEEICHYAKI
                     AHVYDDIMGFPEGFDTKVGERGVTLSGGQKQRISIARALITEPKILILDDSLSAVDTQ
                     TEESILRGLSEFMHDRTSIIISHRISTVKDADEIIVLDDGKIIERGTHASLLAQAGLY
                     SEIYEKQLLEEELASRS"
     gene            46550..48751
                     /locus_tag="PLU15_00185"
     CDS             46550..48751
                     /locus_tag="PLU15_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29370.1"
                     /translation="MNGYEEEEQIIGKVYDSQLIRRLMSYAAPYRKTFVLGIIVIMII
                     AGTDLVRPYLIKTAMDDYISGYDAPMARYEPEAVPAGIRDNRMIHYNGHVYVRIKDLP
                     SNHADEYEPEALFQLLNISDQYVLISGALPPDHGPAQLATTDSGLVLIVDEVEHPAQI
                     IEGHENIGIFREQDLAAVSRLAIILLLVGALAFVLNYGQTVLLQRAGQRIIYEIREEV
                     FTHLQHMDVAFFDTNPVGRLVTRVTNDTETLNEMYTSVIVNLFKDVFMLVGIIVVMFR
                     LDTQLALLSLAALPLIIVSTIIFRTRARQAYRQVRTALARINSTLAENISGMKIVQIF
                     NRQQRNYDDFVNVNREYYRAGIGEMMVYAIFRPIMELIGTGALALVVWYGGRNVISGT
                     MQFGMVYAFINYVSMFFGPINDLTEKYNLMQAAMAASERLFQLMDTEIMVKDPENPVS
                     VDRLDGRIEFKNVWFAYNDADWVLRDINFTIEPGEVVAFVGATGAGKTSITSLINRLY
                     DIQKGQILIDGVDIRSYRVADLRRNIAAVLQDVFLFAGDIKSNIRLNNVDISDEKIKE
                     VARYVNADHFISKLPNGYDEPVTERGSTLSAGQRQLLAFARALASDPSILVLDEATAN
                     IDTETELLIQDALPKLIAGRTTIVVAHRLSTIQHADKIIVIHKGRIREEGTHQELLAK
                     RGLYYDLYRLQYKDELMPEPELDMQDTSAAGCARTAGIASSDSADKTDDMA"
     gene            complement(48677..49378)
                     /locus_tag="PLU15_00190"
     CDS             complement(48677..49378)
                     /locus_tag="PLU15_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOO29371.1"
                     /translation="MGMKLIAFDMDGTLTQEPSSWEYVHRRFGLWTGRAEAHMMRFLS
                     GEITYEEFSILDAQEWKGMLHAEIERTLGEIHYARCAQEVVDAARSHGAFTALISSGI
                     LALARRVASDLGIDRVFANELVAREGVMTGDVVINVSIDEPHMTKGAVLRRLKMELGA
                     DTDETWAVGDNWGDIGMFREAGTAVLVAPTPEDREKVVVAVPNVVCVPSLCHVVSLIS
                     RIGAGDSCSPGATGG"
     gene            complement(49551..49784)
                     /locus_tag="PLU15_00195"
     CDS             complement(49551..49784)
                     /locus_tag="PLU15_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOO29372.1"
                     /translation="MDQNRLSDRDIAADLLSDLKELALGYHMATVEASNQSVRETFKN
                     SHDDWMNEQRGLWQTMYSKGWYQLPQTGPGKGM"
     gene            complement(50130..51224)
                     /locus_tag="PLU15_00200"
     CDS             complement(50130..51224)
                     /locus_tag="PLU15_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOO29373.1"
                     /translation="MRIGIVGMPGAGKTTLFRLLTGVAPSRKQDASIGMARVPDARID
                     LLSKMYQPKKTTYAQLEVVDLAGRASSGSTFDDFSSNKREIARGLTLLINHMRNVDAL
                     VNVVRTFDSLELGEPNPLADASALTDELILADMAIAEARIERLNSSKKRTEEEDKELA
                     LMERLDEEFGAGRSLRDIQLEPEEAAGLRGYGLLSVKPIVVVANMSEEQFREGKFPRD
                     SQLQEYCCERSIPLIRLAAEIEAEIAELDSDDKDLFMAAYGIEVSGIEQVSRAVYDSL
                     GLVSFFTVGEDEVRAWPIENGTTAKRAAGKIHSDIERGFIRAEVVACDDLMSAGSLAD
                     ARSAGSVRLEGKEYVMADGDIVNFRFSPAH"
     gene            complement(51329..53002)
                     /locus_tag="PLU15_00205"
     CDS             complement(51329..53002)
                     /locus_tag="PLU15_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOO29374.1"
                     /translation="MESSIRVTLPDASEHNVASGISLEELAKIIPHGLRWPVMAAKVD
                     NNLKELTYVLDTDAHVEFVDMGDSDGMRVYTRTGILVLVMAAREVLKDCRVIIQHSLS
                     NGLYGEIKWTRPLTEAHVRAIEQRMREIVEADKPINRKRMLVEDAIELFRVDGQMDKA
                     RLLRYRKADTVNIYECGWLSDYLYGYMAPSTGYFGTFRLRHYLPGFILELPRRSNPLE
                     LPEYVEQGKLANVYFEAEKWGRVLGVNDVASLNDVIRAGNIDALIQIVEALHEKKVAQ
                     IADVIAQNRDRIAVVLIAGPSSSGKTTFAQRLWVQLRVNGLNPVPLSLDDYFVDRANT
                     PVDEEGEYDFETIEAIDIPLLNEHITRLIQGEEVEVPVFDFIEGKRSSKGRPIKLGQD
                     QMLIIEGIHGLNDELTRSIPMGRKFKIYASALTHLNIDDHNRIPTTDVRILRRLVRDN
                     LFRGHSAKDTIRMWPKVRAGEERNIFPFQETADVMFNTALAYELAVLKPYADPLLAAI
                     GQEDPEYIEAKRLRKFLAYILPVERADIPPNSIMREFLGGSCFGRMRDA"
     gene            complement(53074..53856)
                     /locus_tag="PLU15_00210"
     CDS             complement(53074..53856)
                     /locus_tag="PLU15_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29375.1"
                     /translation="MNVQAVWVEIKKGLIRKIRSPVELITETLAAYVFLTVAIMAASG
                     RAVTSGDAADELLFSLACVFFCMGALQGVVRIVTEEPGSGVVEQLSSSSIPLWRIALI
                     RDIASLANFLPNIAVIILAVSLTTGARMPPPKLSALVPLILMRLGMLGIGYVLGGIAL
                     LAKRIGGLVNLISMGLFAVAFVPVDAKKGSVAAVSRFLPYGGCYYMAREITFGGVGLG
                     EFVAGGDLASAAILAGVYFVIGVFAFNKAFEAALDGGFLGKS"
     gene            complement(54331..55347)
                     /locus_tag="PLU15_00215"
     CDS             complement(54331..55347)
                     /locus_tag="PLU15_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29376.1"
                     /translation="MSDLVVDMKSARKVFRRRRCDKPVVALCDLSLAIPKGEILGLLG
                     PNGAGKSTAIRMMSGLVTPTSGTILIGGYDVVEQRPEALSCVGVVLDRARTLYPRMTP
                     WENIQYFGIMKGLRDTERLRIRAGEWLDFFNIAEVADRPVNDLSMGTRQKVSLAVALA
                     AEPRVVLLDEPTVGLDVMSSLELRKAIKRMAHEEGCTVIMTTHQMDVAEDVCDRVCIL
                     NEGRVLALDTVRRLLDVFAFRAYTLTLAGSLTEEVRTALSGLGQVEVREKWLDGFGVS
                     HSGYTVIDIDVPDERGFFDVMGVLELAAVGVESVTRREPCLESVFLKLAGEEDGSFRQ
                     PSYA"
     gene            complement(55735..56979)
                     /gene="pepT"
                     /locus_tag="PLU15_00220"
     CDS             complement(55735..56979)
                     /gene="pepT"
                     /locus_tag="PLU15_00220"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOO29377.1"
                     /translation="MNKDALMQEGVAEKFMRYVRVNTESSDETDTTPSTEGQWDLARM
                     LAEELRALGLEDVAVDEHCFVIGRLPSNLDKPAPAVGFMAHLDTVPGIPGEGVKPVMH
                     RGYAGGEIEIGHGVILSPEDSPALAAAIGCDIITSDGSTLLGTDDKAGIAGIMQALVE
                     LIRNPDIPRPDVWIAFTPDEEIGKGVARFPYDKWGAKYAYTLDGGYLGELSEETFNAI
                     NGVVTIEGVSSHTGTARGKMVNAVHIAAELISAIPADWRPETTSGREGFTHPNEIEGN
                     VEKVSIKVIIRDFDAKLLEKRETAFIKTLEALEANYPGCSIKWAHTGGYRNMKEKLDE
                     DPRVVRLALEAMEMANVDPVVAPIRGGTDGSRLTCEGVLTPNLFAGGGDMHSRREWAC
                     VQWMEKAAEVVVNLCRLWAEEE"
     gene            complement(56994..57497)
                     /locus_tag="PLU15_00225"
     CDS             complement(56994..57497)
                     /locus_tag="PLU15_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29378.1"
                     /translation="MRALLVYHSRTGATERLIKSIGRALEGRGHRVEYHKLEAKRPMG
                     WFASSWAAFRKRETDLAEDMPKSLRMYELVLIAGPVIAGKTSAELNAFLNNMPDGIGR
                     RFGVFATSGSGAAEGPLRSMKSRIEHVGGFIIYEDVVKRQIAMDPEACTRRAEEIAEQ
                     LVRPWVW"
     gene            complement(57617..58564)
                     /locus_tag="PLU15_00230"
     CDS             complement(57617..58564)
                     /locus_tag="PLU15_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOO29379.1"
                     /translation="MPANLTPQYMEAEEAYKRATTADEKIAAVEEMLRTIPKHKGTEK
                     LQADLRKRLARLRAEGRELRKKGKADPYCVQREGAGQVFVVGPPNSGKSALVGALTNA
                     KVVVAPYPYATPLPVPGMMKYENIQIQLVDCPPISASNCPPALTASMRTGDGVMIVLD
                     ASSDSCLDDLDEQLALLRERKVIEDAKRFLIVASKIDLPDATINADILAEACGEAQLV
                     RVSTTSEHGICELPKIIFAMLGIIRLYPKPPGKEEPDRNSPVTLPAGSTVADLAEAIH
                     RDLPRTMKGAKVWGSTRFGGQFAPREWVLEDGDVVEFRT"
     gene            complement(58598..60538)
                     /gene="pulA"
                     /locus_tag="PLU15_00235"
     CDS             complement(58598..60538)
                     /gene="pulA"
                     /locus_tag="PLU15_00235"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOO29380.1"
                     /translation="MGRETSSVVAVGGQEVFELYYPGDDLGAVYSKESTTFRVFAPTA
                     LGVSVALYSSTEATVADAIEMAPDCDGTWRATVPGDLEGVCYTYRVDLGGVVNEAVDP
                     YARALTANGVRGVVVDLDMTDPEGWENDVRPPFGAATDAIIYEVHVRDFTSSPDSGVR
                     YPGKYLGMADRGSRGPAGVCTGLDHLAELGVTHVQLMPIQDFASVDELDKDGYNWGYD
                     PYHFFVPEGSYSIAPECGIARIIEVKKMIQAMHSAGLRVIMDVVYNHTWSVEDSPLHM
                     IVPSYYHRADCAGRYTNGSGCGNELATERPMVRKLILDSLRYWVEEYHVDGFRFDLMA
                     LMDYETAREIELRLHAIEPSLLLYGEPWAGGLSGLSSHRMFTKGRQRGLKIGFFNDGF
                     RNAIKGDNDGVRRGFATGAGGCGRAIERGVVGGIPYSADIADFATEPGECINYVSCHD
                     NLTLWDKIARSAPLDSEEDRIKMDLLAQAIVMTSQGIPFMEGGAEMLRTKGGHCNSYR
                     AGDAINRFDWVRKLDYAEVFDYHRGLIQLRRAHPAFRMCSAEAIRRSLRFLPAPADNV
                     VVFMLDGASCGDEWDKIVVVYNPNRQDVRIDLPIEGEWKVVVGGASAGVEPLDPDLFG
                     LRTYEGGAAVVAKAISTTVLHT"
     gene            complement(60634..62457)
                     /gene="pepF"
                     /locus_tag="PLU15_00240"
     CDS             complement(60634..62457)
                     /gene="pepF"
                     /locus_tag="PLU15_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOO29381.1"
                     /translation="MSVSMPDGSMEIPERKDVPAELKWKLEDIYASDELWEADVASLL
                     GRLPELASLQGRIGQSAEDLLKGLDLLNEINMIAAKIYVYARMRKDEDNANSKHQAMY
                     ERAASLVVQVSSTASFIRPELLEVDEDTLEEYMAENAELELYRHFLHDISRMRAHTLS
                     APEERLIAMAGEVGEAVSSIFTMLNDADIKFGSIVDEEGNTVELTKGRYTRFMESPDR
                     RVRKEAYDALYSSYGSLINTIGSTYSASVKSDAFNARARRYDTCLEAALDKNNIPVKV
                     YSNLLEAVRDRLGLMHRYVALRKKALKLDDVHMYDIYAPIVPDIEWTVTYEEAKKAVC
                     EGLAPLGDEYVSILGAGLESGWVDVVESRGKTSGAYSWGEYGTHPYVLLNWQDNLDNM
                     FTLAHEMGHAMHSHYSWTTQPYVYSDYSIFLAEIASTANEVLLMEHLLSLTEDQVRRM
                     YLLNHLLEQFRGTLYRQTMFAEFEKIAHERYEAGQALTPDALNDIYLQLNRDYYGDEI
                     VLDDRIALEWARIPHFYSAFYVYQYATGFSAAVALAESILEHGDPARDRYIEFLRGGR
                     SKYPLDLLRDAGVDMTSPDPIHRALDVFEKSLEEMETYFRG"
     gene            complement(62795..64438)
                     /locus_tag="PLU15_00245"
     CDS             complement(62795..64438)
                     /locus_tag="PLU15_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOO29382.1"
                     /translation="MERSMIFGIIGGLGLFLYGMNLAGDGLRRAAGDRMRNILEVLTS
                     TPLKGVLVGMLVTAVMQSSSATTVMLVSFVNAGLMTLRQALGVIMGANIGTTITAQLI
                     AFNLADYALPAIGIGFLIHMVARRKVWKSVGQIVLGFGILFLGLSLMSTSVIPLQDSP
                     AFGKAMKTFGTNPVLGVLIGLVTTLVVQSSAATIGMLMALAISSPDLVTLEVAIPILL
                     GDNIGTCITAILSGIGASVTARRTAMAHLMFNVFGALIFLPFIGPFKWVVEAVTRSGD
                     IQRQIANAHTLFNVANTVIWLPATGFLERVVSWLVPGEDTVVEIGPKYLDRRMLGTPP
                     IALDLAVAETVRMGEIVQEMLDYSRKALTQGYTSQLDSDLESREQLIDDLNREVVLYL
                     SMVAQSSLNEAESRRLAGLMHAVGDVERIGDVAEQLMFYAREKFDQGLTFSETAMKEL
                     EGVFDLAEQMLARGVVALRDNDMEAVEDVYNIHKKLDETTNMLRANHIGRLNEGKCVP
                     GSGVIFVELMNSLERMGDLSVNLADAVSGKKSPRVEPRS"
     gene            complement(64443..65696)
                     /locus_tag="PLU15_00250"
     CDS             complement(64443..65696)
                     /locus_tag="PLU15_00250"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO29383.1"
                     /translation="MPRDRHVLHDVYVWSLVALSVVLVGRFIEWDMPLGSALAFIGLL
                     WASSLSVINLRNTVVLMLLPIMMTPVVKYNPGATAVIAAIGSVSIQEIRILRENNSPY
                     LFILKVLGNRASIMLCALLSGLVFHSLWPAATVDFGDVRFLAAFAIAGLSWVMLSGVI
                     INFQILLSEPKFEVKIDPAVIIGSSRSLVPSVIMGLMAMAMYEYGGLILFTLAQLFFI
                     SNKNYTQRALVDKENAEQINLALARIIDSKDHYTGGHSERVAILARQLAEACRLPGAD
                     VDRIEYIARLHDVGKVNLPDEILSKPGRLTAEEYDAVKRHPEWGAELIRGMERIYSDR
                     DYRAILEHHERYDGAGYPYGKKGEEISLWGRILAICDAYDAMTSERVYREALPVEAAR
                     FELKRNSGTQFDPELVDLFLSNVQE"
     gene            complement(66004..66651)
                     /locus_tag="PLU15_00255"
     CDS             complement(66004..66651)
                     /locus_tag="PLU15_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29384.1"
                     /translation="MSDDRKTRLAKAEGRMRLLPGVTRVRILELADGDEIHVMVEGCY
                     DQDEIKRLKKDIETVYLLETGESIDYRRISIAQIREKHPDGHAGSNGRPVLSRISVEY
                     GQGGAVVAYVDIEFGGECYSGSANGRTIEEPLSNIVKRATLKSIEKLLGEKYALQADC
                     EIADGRVISEITLIDSSSGQRQRYLGAAYKRDDISTAVARSILQALNRQFELLVD"
     gene            67391..68278
                     /locus_tag="PLU15_00260"
     CDS             67391..68278
                     /locus_tag="PLU15_00260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO29385.1"
                     /translation="MYKLIACDIDGTLLRSDGSTSPRTIKAIRLAQACGAKVSIATGR
                     RIISAMPVIDAFRMVHPAVVHCGAVVLQPGSESILMAQQIPRDTALHVCHIAENCGVD
                     VTVFESVYKGRALYVTPFAISDCAQPLWPRTLPECREVATYDEACTSDPVQLCIQGNI
                     DAVDRFLDKIDADFSNQVSFFDYGVLEDGFRVVDVFAAGVHKWMGVSFLADMWHIRPS
                     ETIAFGDSLNDEELIAHAGLGVAMGNSPDELKELADLVAPTNDEDGVAVVIESLARRG
                     LLGHEDSKSCGEPSEEGTL"
     gene            complement(68301..69110)
                     /locus_tag="PLU15_00265"
     CDS             complement(68301..69110)
                     /locus_tag="PLU15_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOO29386.1"
                     /translation="MRRQWVAVSIAVGVIAASVLLCASGGRAPSETTPVNKATGHVLG
                     RCITLLNADEKERNANVALGARKLDGTVIDAGTEFSFNASIGERGVEQGYKPARVIVD
                     GKNESGIAGGICQLSSTLYNAVLLSGMEVTERHAHSRPVPYLPAGLDATVSYGARDLK
                     WVNTSGSPVTIRAEASGDRLEVWLEGEGPAPDIRLFTEIVETIPSRPMSPPDATGRSV
                     APPRPGASDKIRRGVEGYRVRVWAEMPTPGGGRVRELVSEDLYEPVHAIVD"
     gene            complement(69107..73666)
                     /locus_tag="PLU15_00270"
     CDS             complement(69107..73666)
                     /locus_tag="PLU15_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOO29387.1"
                     /translation="MRCLGRSARVSHAIVLAALLALFMAPAAMSAREPQVWLFATARN
                     YLPENEPELGIRGYGVKELWLDIYKFDGFRHFKNGDWSALWNVNYKDLPGREKVRSVR
                     VDIARPGGTLDAAVKLKPLPPGQYVAVARSGQLKTEEAAWFAVSELGLISKQYSRGLV
                     IYAVNLRTGEPMPNVSVYAKLSSMSGSDASERFDQETVESGEKGDIGGAAVVSVGSVS
                     VPGEVYGMTDNRGLFTIDLADSADSAFVVAAKGDDFAVLNSSYWWDARTQKVYVYTDR
                     PVYRPNNVVYFKGIARGITNSGYEVLARRPVRVEVRDPRNNVVYKQDLLTNDWGSFSG
                     EFKLGAEPPLGHYWICAIVDGEQHEGSFQVAEYRKPEWVVEIEFDKEMYIAGDRLDAT
                     CKALYYFGAPVRNARVAYRVYRQPMGFFGPTAEDSGYDYDQYGGVDDMVGLYYGDFVL
                     SGETRTDSDGLARISFESPAADGHNYRYIVEADVIDATNKRAYGRGYTDVARGTIDVD
                     VSTDRYVVREGDKFEVYAKAVDLDGNGVVRDIDIIVLKRSYSKKGYTDTPVHSTQITT
                     AASGDGFIELEAGKPGDYVIQAKSEDERGNAIIAETYIWTTPDNGSWADMFRTDLGIV
                     CDRGRYDVGDVAKVLITAPPEVSSVLLTVEDREVRDVRVIRLENGSAEVELAIKPEYA
                     PNTYVSVVAIVSGVMQTAARQIVIPVPERLLNVEIKPNKDSYRPGETATYLIVTENSD
                     GDGVPAEISFGLVDESIYAVRPDTTPAIDKFFHGRRERTVSTENSFPVTYYGGAEKEG
                     GTEATRKYFPDTAAWFPSVVTDSNGHAVVRVKMPDSLTTWRATVRAHTRSTIVGQAQE
                     KVTVSKPLSIRLGIPRHYTMGDCGRVTGVIHNETKWQRIVFVNLNASGAKIVGCSWQW
                     VKVPASGEAVVEWEVEPETVGDVVFKANAWSWFVGDKLALSVPVLPFGEKCDEVWAGE
                     VRPDGGRGENDIEFDLPDDIVQGATSLSVDISPGYAGVVESALEFLVNYPYGCVEQTM
                     SAFLPDILAHRAFACMGIPMPRTEEELADMVNSGLARLYKFQHWDGGYGWWEHDDSGL
                     WMTSYVLYGLERARRSGFCVSEEAMARAADYLREAVWDEKSDTNRAFACYVLAEQGAL
                     GAKGMKALEQLAAKLPAYPNVEHGRRVAAESQSLKPLTVAYVTLAASAAGRDDMASAG
                     AETLRNMATVISGQAFWETEDKENWWRNETGETTAWAMMALLEAEPSSPLLSQAARHL
                     AMTRDGSQWRSTRESAAAIMALVEYMTAMEEDVRQRRQVTVEVNGRRIAKEYLGGDSG
                     YEAANLCVKIDPEFLIVGRNSVKISAEGGPVYYSINASWHEARDRIEAAGECADIVRT
                     YFVIDRNAEAPEGEEYALIPVDEDTPVMCGQELLVRVAIDAKRDMDYMIFEDPVPSGF
                     EISEGFTDPWSWNYWYDRREVRDDRMVFFATWIKAGERMVFDYILRPERLGRYMVMPT
                     RAWSMYYPGLRANGASRVIEVADGE"
     gene            complement(73685..74452)
                     /locus_tag="PLU15_00275"
     CDS             complement(73685..74452)
                     /locus_tag="PLU15_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HOO29388.1"
                     /translation="MRLFLSRKQHMFALMVACIACAILAYAGVVGDNEMPASQGAADF
                     ISAVDMPAAGAWDVLSELNMVGVSNVEGPVIAIARECVALSVRGQPVPSATELIMSVS
                     AIPEVEQALTRRSAGVFVTVVRNNSVRACVGSIWPRCANLAEEIAQSAREVAARDLRR
                     PPIEPWELPTCEYAVSVVGRLERVSPDANWDPRAFGVFVRAGHRSGVILPGEALTHSK
                     QLSWARDEAGIEPDEECEIYRFQTVKFGCSLDLGRGR"
     gene            complement(74583..75593)
                     /locus_tag="PLU15_00280"
     CDS             complement(74583..75593)
                     /locus_tag="PLU15_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO29389.1"
                     /translation="MRTENSSEDRFKIGARVSWGNMIANLGLVAFKLIAGIVGKSSAM
                     VADAAHSASDLLATAVVIMSLRVSKRPADVDHPYGHGKAEPVAAKIVGLILIIAGCAI
                     GSNSARKIAVGGYTEPGKIALVAAVVSIVVKEAMYRITAATAEAIESSAVMAEALHHR
                     SDAYSSVGTFIGILGARLGVPVLDPIAGIVVAALIVKMGFEITRKSSDELMDAQADPD
                     TLRAVQEAIDSVEGVSDVHYMAARRYGGYAHVDLRIGVDPSLSVVEGHNIAVAAEKAI
                     KGVCPHVIGVMVHVDPESVHEDDPIGHGYADVFQPEGPQRIAQPNAAEMTSVVEPSHI
                     RE"
     regulatory      complement(75661..75757)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            76109..77776
                     /locus_tag="PLU15_00285"
     CDS             76109..77776
                     /locus_tag="PLU15_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO29390.1"
                     /translation="MKTNRPTSVAAPSNGKTVSFAVLTVACIFIFFGSATAGAESGLH
                     APYLIIHVDAVPSRLFFEEYDAGHLPNIKAVFDDGKLVRYAVSPFFAGTEMLYPRLKT
                     GAGPDQPLPITWEMYDRNSGRHISRIETQVILFSHLPTYSLTQIIHGIPGMDYLSGWA
                     LLNTPRLLDEYDIVEFFWFITDSYGHVFGEEAQRESLLILDEFLGILMKQLGSKPVNL
                     VIYADHGMSMFERTYDAESAMAETAGDNEQFVYYPNLYLKDRTLAATTAEQLANRDEI
                     DFAFYRADEFLIIGIHKEGQVMFRSEGNLITYSCEGPDPFGYADLGCLGRPLSDQDWL
                     EMTCTSRYPAVPVQIVRYMDNALSGDIVAVLNPPKGLRTTVGFCGCHTGFVDTDCVVG
                     VLMKGPDIDPSQIPDTFWLYEMYSQVLKIDPQNPTKADRAKNFFMVSDGGIDLQLSAF
                     RNNTALSIGPQEWTFIGETTIARSFITQVRAGLGLASDKSLAGGLTPVASLRLDIYFG
                     PLTYDHRNTIALTDGCRKSTSTLRFEAKNGVVLEWEYPNRIKLGVSW"
     gene            78354..78686
                     /locus_tag="PLU15_00290"
     CDS             78354..78686
                     /locus_tag="PLU15_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO29391.1"
                     /translation="MRLHGPDEPVYTIGVAARLLGVSPQTLRMIEREGLLEPARTEAN
                     TRLYSENDLLLLQRICFLVREEGVNLAGVKMILEIESKQGNTTIRYREVTQSVPREAK
                     PGEGNGNS"
     gene            78743..80692
                     /gene="dnaK"
                     /locus_tag="PLU15_00295"
     CDS             78743..80692
                     /gene="dnaK"
                     /locus_tag="PLU15_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOO29392.1"
                     /translation="MGKVVGIDLGTTNSVIAVMEGGNPTVIVNSEGARLTPSVIGFSK
                     SGERLVGQLARRQAVMNPENTVYSIKRFMGRRFDEVEEERNRVPYHVKASSNYGIKID
                     VPATGRDYSPEEISAMLLQKLKTDAEKFLGEPITKAVITVPAYFNDSQRQATKDAGRI
                     AGLEVLRIINEPTAAALAYGLDKKKNETILVWDLGGGTFDVSILEVGEGVFEVKSTAG
                     DTHLGGDDYDQRLVEYIADEFQRDQGIDLRKDRQALQRLIEAAEKAKIELSQVYETNI
                     SLPFITADQTGPKHLETKITRAKFEELTSDLTERCTRPFKQALSDAKLSLDDIDEVIL
                     VGGATRMPVIQELVKRLTGKEPHRGVNPDEVVAVGAAIQAGVLAGEVKDVVLLDVTPL
                     SLGIETLGGVSTKLIERNTTIPTRKSQIFTTAEDGQTSVEIHVLQGEREFARDNRTLG
                     RFTLQGIPPAPRGVPQIEVSFDIDANGIVNVAAKDLGTGKEQRITITASTQLSKDEVE
                     NLVREAERAAAEDKKRREEVELKNSADSLVYVTEKTLREMGDKVPAETRSKVEAELEE
                     LKKAIADDDMESVKRFMDQVREASYKMSEEIYKAAQEASGGQGEGDYGSDSGDATGAG
                     GFDSANSDDDDVIDADFRTVDGDDN"
     gene            80831..81277
                     /locus_tag="PLU15_00300"
     CDS             80831..81277
                     /locus_tag="PLU15_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO29393.1"
                     /translation="MALMNYDPWQEFTEMRRVFNRMFGDGFLGLPMEEQSTHGSLLMP
                     VDIAETPEEFVIMAELPGMDPDDIEITLTDNQLTIKGQRAAEYEDKRENYLRTERRFG
                     SFCRSFALSSPVAEDKVSANYKNGILEVHLPKSEGAKPKQIEIRNE"
     gene            81413..82009
                     /locus_tag="PLU15_00305"
     CDS             81413..82009
                     /locus_tag="PLU15_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOO29394.1"
                     /translation="MSPDTQDMRLGNIQSSNSSEVTGAEANGEHTPEDLPSNADMVNE
                     GDELRLKLAEAEAKAEENWNMYLRALADLENYQRRTARDLEFTIRSGKKEMFLKLLAI
                     VDDMERALAADADYESLHQGVEMIMRKLLDALASEGVKPIDALNEPFDPRLHEAVAAC
                     DGEDVETETVVEEFRRGYTYCNECLRPTMARVAVPKSD"
     gene            82053..83033
                     /locus_tag="PLU15_00310"
     CDS             82053..83033
                     /locus_tag="PLU15_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOO29395.1"
                     /translation="MEFKDYYKVLGVSKDATEEEIKSAYRRLARQYHPDVNPGDSSAE
                     ERFKAIAEAYEVLGDPEKRKRYDMLGADWDHYSKTSAGWPPGGRRTQPGYGRTTFTFG
                     NLSDADFSDFFKQFFGDLGLGDHLFGHDLHDLDERSFSRRRPVKRDIEHELEIGLDEA
                     FTGSQRSIEIVDPTDGSRRKIVVDIPPGVRDGTRLRIAGEGAGAPGGRGNLYLKVRIR
                     PHRGYQLRGDDLYVKVNVPLTVAVLGGEVQLQTVTGDTISVRVPPETQNGQVLRLRGL
                     GMPSTKGGKRGDLMVKVNVVLPSNLTEQERELFRELERLTSASSSGRDEA"
     gene            83030..85627
                     /gene="clpB"
                     /locus_tag="PLU15_00315"
     CDS             83030..85627
                     /gene="clpB"
                     /locus_tag="PLU15_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOO29396.1"
                     /translation="MRFEELTVKSQEAIARAQSIAQANQHQSVAPMHILAALLEQEEG
                     VVPLILSKLGVSAEVLTRTVASSLEKLPKVYGQEAGQYLSTATASLLDDASSEAGRLK
                     DEYVSTEHILLALAGADDDSGRLLRSMGVTKDAIYKALVDVRGTHRVTDQNPEDKYQA
                     LERYTRDLTDLARKGTLDPVIGRDEEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIAAGDVPDSLKNKRVLSLDLGALVAGSKYRGEFEDRLKAVLKDIEDAEGQIILF
                     IDELHTLVGAGAAEGSIDASNMLKPALARGELRAVGATTIDEYRKYIEKDAALERRFQ
                     PVMVDEPSVEDTIAILRGLKERYEVHHGVKIKDSALVAAAVLSDRYISDRFLPDKAID
                     LMDEAASKLRMEIDSMPAEIDEVERRIVQLEIERHALDKEDDESSRERLIRLNEELKE
                     LKEKSEAMKAHWQKEKETIARIREIKQKIEEAKIEEQAAERAGDLGRAAELRYGVQLS
                     LQQELDRANQEIAELQKEQRFLKEEVDDEDIAEIVAKWTGIPVTRLLEGETEKLLKME
                     QLLRRRVIGQDEAIEAVSNAVRRARSGLSDPERPIGSFLFLGPTGVGKTELAKALAEF
                     LFDDERAMVRIDMSEYQERHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEI
                     EKAHQEVFNVLLQLLDDGRLTDGQGRTVDFRNTVIIMTSNLGSQHIMEHATGSEEQMR
                     DLVMEVLRQAFRPEFLNRIDEILIFNRLGREEISQIVQIQLGLVRDRVASQGFLLEWT
                     PQVRDFLAETGYDPVYGARPLKRAIQRELENPLAMEILQGKFAPGDRIKVVLTDAGEN
                     GGALAFQKA"
     gene            85951..87300
                     /locus_tag="PLU15_00320"
     CDS             85951..87300
                     /locus_tag="PLU15_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO29397.1"
                     /translation="MTGKIGKYHGTTAGAFLYSLGSFSAALLGQAFNAWVQLYYVDIL
                     KMPLALYGTGMALYGIWNAINDPLAGQWSDNTRTRWGRRIPFILFGTIPFCIIFALVW
                     WVPQWAQASSRSLFAYFLVTAFLFDGLFTVVVLNWTALYPEMYPSLTQRSQVSALRQA
                     LGIVGMIIGMVLAPQFRQWFGWAGMGILFAVIGGLSMFLSLLGSKENPDFSEQQGLRV
                     SEAFKHTFVNKSFVTFVLASLLIQFTFVLLPATMPFFCKYVLGLTDAQNTLVLAPIFL
                     VALPMVWVWGRVTNRIGPRRTAMVAAVVFAVALIPFGYVTTLLGGMITSAFAGIGLAG
                     LMVIIDVLIADVIDEDELGTGTRREGAYFGANAFVMRLGVSLEAITFSAIMGMHGYNP
                     NLASQPSTVIPGFRILMTVVPIVSLVLALIALYYYPIDGDRREAIIQGIEKKRSAEG"
     gene            complement(87830..89284)
                     /gene="gatB"
                     /locus_tag="PLU15_00325"
     CDS             complement(87830..89284)
                     /gene="gatB"
                     /locus_tag="PLU15_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOO29398.1"
                     /translation="MTSDYEVVVGIEVHVELATESKVFCGCRTAFGAEPNTQTCPTCL
                     GMPGALPVLNKRALEFAIRAALALNCSVADYTKFDRKNYFYPDLPKGYQISQYDCPIG
                     SNGYMDVDVNGESKRIRIKRVHLEEETAKLMHAGDDIISASHSLLDFNRSGIPLIEIV
                     SEADMRSADEARAYLTKLRSILLAIGVSDCKMEEGSMRCEANISVMPRGSEQYGTLVE
                     LKNIASFRAVHRAIQYEAARQIEIVSSGGEVARETRHWDDAKGETAFMRGKEEAHDYR
                     YFPEPDLGPIVIDRAWVEKIRVELPELPDAKAERYMREWDLPKYDACQIADSADLSDL
                     FEKTCAIVGEGREVAKWILGEVMASLNATGIELSESKLTPELFAELIDLMTTGKISGK
                     IAKSVFEDVFSNGRRPSDIVKERGLLQISDVGELVTIVEKVIDANPGPVADYHAGKER
                     ALGFLVGQVMKETRGRANPQETNRILREELDKRA"
     gene            complement(89281..90783)
                     /gene="gatA"
                     /locus_tag="PLU15_00330"
     CDS             complement(89281..90783)
                     /gene="gatA"
                     /locus_tag="PLU15_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOO29399.1"
                     /translation="MKLCDMTIHELAALLRAGEVTSTEITESVFDRIDQVEESVGAYV
                     TTARDQAFEQAAAADKRLLQARKRGSLSDISPLMGIPLALKDNICTRGIRTTCASKML
                     ADFVPPYDATVARRLGDLGAVIVGKTNMDEFAMGSSTENSAFHVTRNPWDFERVPGGS
                     SGGSAAAVAAGEAIGALGTDTGGSVRQPASFCGLVGLKPTYARVSRYGVVAFSSSLDQ
                     VGPITRDVWDCAIMLRAIAGRDERDATSSPEPVPDYCGSLKETDRDFTIGVPSEFMHS
                     GLDSGIRELIDNAVRALEDAGARIVEVSLPHSDYAIAVYYLIATAEASSNLARMDGIR
                     YGFRAEDAHGVQAVIRQSRSEGFGPEVKRRIMLGTHALSSKHYDEYYLKALKARTLIL
                     GDVKEVLEKCDCIISPTSPTVAFRIGEKLDDPVALYLMDVYTTIANLSGMPAVSVPCG
                     TLKGLPVGAQLIGRAFDEGTLFRIAHLLEQACGESVKRPPLEAMKGGRDR"
     gene            complement(90780..91094)
                     /gene="gatC"
                     /locus_tag="PLU15_00335"
     CDS             complement(90780..91094)
                     /gene="gatC"
                     /locus_tag="PLU15_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOO29400.1"
                     /translation="MSELSISKEETAHIAELARLSLNESELAQAQHDLSEIIDYMNKI
                     AGLNTDGVALTIQPCPVANVWREDVVLPSLNREEVLANAPDRQGEFFRVPRIIEAEGA
                     DE"
     gene            complement(91109..93145)
                     /gene="ligA"
                     /locus_tag="PLU15_00340"
     CDS             complement(91109..93145)
                     /gene="ligA"
                     /locus_tag="PLU15_00340"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOO29401.1"
                     /translation="MAELTDAASRVDELRELIRHHNWRYYVLDDPEITDAEYDMLMRE
                     LIALEEAHPELADPDSPTQRVGGAVSAGFEPVVHAIPMLSLDNMFDEGSLRAFDARIK
                     RATGLAKVEYVAEPKIDGLAISLTYENGRLAVGATRGDGERGEDVTHNLRTIRAVPLV
                     LPKGAPDSVTVRGEVYMRKSEFERLNQERASRGEPMFANPRNAAAGSLRQLDPKVTAS
                     RKLDAFFYYLPEAERYGIETHWDALDFIRSLGFRVNPEIILCDGLGETLEFIHRLADL
                     RPMLPYEIDGVVVKVNRLDLQRKLGFTSHSPRWASAYKYPAQQVQTRVRDIIVQVGRT
                     GVLTPTAVFEPVDVGGVTVSRASLHNEDYVRAKDVRVGDAVIIQRAGDVIPEVVRVLV
                     EERTGDEKEFEMPRVCPACGSEVLREAGEAATRCISLSCPAKIREGMIHFASKGAMDI
                     EGLGPSTIDRLLDAKLVADVADIYGLRLDQLAGLDRMGEKSAANLVASIEQSKSNPLY
                     RVIFALGIRHVGENVAKLLASHFHSMDALIAAERDDLLAIPTIGPAIADSVVSFFSSE
                     MNLETVKKLAAAGVRMSDEPEEAKPAAVEFDGKSFVFTGTLASLTRQKAEEAVENLGG
                     RVSSSVSRKTDYVVAGENAGSKLARARELGVAVLSEQDFAEMLERAGCRLGDER"
     gene            complement(93158..95422)
                     /gene="pcrA"
                     /locus_tag="PLU15_00345"
     CDS             complement(93158..95422)
                     /gene="pcrA"
                     /locus_tag="PLU15_00345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOO29402.1"
                     /translation="MGGDLLGALNEPQREAVVCVDGPLLILAGAGSGKTRVLTHRAAY
                     LISERRVPPYRILAVTFTNKAAKEMKNRVEELVGARADLMWISTFHSLCARMLREDIP
                     SLGYKKSFTILDESDQTAVVKKCLDDLNISGDMFKPQAVLAAISSAKNELIGPEELEA
                     NARDPRTRTMGQIYAAYQSTLVRGNALDFDDLITLAVRLLRECPEILEKWQDRFEYLM
                     VDEYQDTNHAQYILVKLLAARSRNLCVVGDDDQSIYEWRGADIRNILDFERDYPEARV
                     IKLEQNYRSTQNILNAANCVIANNKGRKDKSLWTSRGSGSPIFVYCAENERDEGRFIA
                     DEIQRLAALEGRAYRDFAVLYRVNAQSRAIEEVLLNRGMPYKIVGGLKFYERREIKDV
                     LAYLRVIENPEDSLALERIINVPRRGIGDATLAIVRRLSEEAGVPLYAGVELAVRASD
                     TPSRARSGLSQFLQLMEGLQSRKGEATVSQLVEMVLEKTGYIRELEREQTLEAEGRIE
                     NLRELLTVTQEFEARRMENSVEDGGGPTDIGAFLEEVSLLTDLDRADLSDDSITLMTM
                     HAAKGLEFPVVFIAGMEENIFPLARSAREERSLEEERRLCYVGITRAKDRLYLVHSVM
                     RMIYGSTMANAPSRFLGEIPKELLADAMHDRRSLWGRSDGPASPVIRAHSVEGRSGTA
                     WNRGDSSDGGGENHGGDGSAEYSAGDRVRHAKFGEGTVVAVARGGNDWVITVAFPGGG
                     IKHLAQSFAPMERI"
     gene            complement(95427..96905)
                     /gene="gltX"
                     /locus_tag="PLU15_00350"
     CDS             complement(95427..96905)
                     /gene="gltX"
                     /locus_tag="PLU15_00350"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOO29403.1"
                     /translation="MNADSTSSKDRKVRVRIAPSPTGNCHVGTARTALYNMLFARKYG
                     GTFVLRIDNTDLERSTKESEEGVLEGLRWLGIKWDEGPDIGGPYGPYRQLERMDTYKE
                     TVEKLLSEGKAYYCYCTPSELEEERKAALAAGKPPRYSGKCRELSSADRARLEAEGRR
                     PVVRLRVPDVTLSFDDLIRGHQQKDMSERGDFIIFKSDGTPVYNFATVVDEHYMEMSH
                     VFRAVEHLTNTFDQVCVYTAMGWEPPQFGHLTLMLNPDRTKISKRAGAVYIGEFREMG
                     YLPEAVINFLALLGWNPGTEQEIFSIDELVEAFSVDTLSKSDAVFDMKKLDWFNGVYI
                     RSLTVPDLASRVRPFMEAAGYSVDDTDYLERAVALITDRIGKLTEAPDLLGFLFVDDV
                     ELDPSALKTKAKFSYDDIARGLKASADALEKVSTWEHEPIEEALRGTATELGWKAGEL
                     LMPVRIAVTGRRVTPPLFESLVLLGRDRSLARIRKAIALLEE"
     gene            complement(96930..98285)
                     /locus_tag="PLU15_00355"
     CDS             complement(96930..98285)
                     /locus_tag="PLU15_00355"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO29404.1"
                     /translation="MVGQERAQEIVEAAISACRADAVEAVLMSDVDNLTRFANSEIHQ
                     NASVSSDILYVRAICGKREGWATTNQLTSEAVRDAAALAVEIAMVQPENPDFPGLAGL
                     CRYEEAGGYDERTASFSARERAMAVKAITAIADKHELNASGAFRTRVTGMAIANSEGA
                     SAWMRGTDSAISAVIMTGYDSNAGSGYAEAASWSAGNLDARAIGERAVEKCLSSRNPG
                     ALEPGEYEAILEPAAVVDMMETLALMGINGISFIEGRSYASGRIGSKITSDMVTIEDN
                     WHDPGLLPRPFDLEGVPRRPVTLIEHGVASGMIFDRLAAKKAGTESTGHALVGGALKG
                     GYPLHLRMSPGESSIGEMVASTGRGVYVTRFNYTNVVHPTKTIFTGMTRDGTYLIEDG
                     RIVRPLRNLRFTDSILDGILANIVAVSRDTELLPVAGGRGIYRAPAIKTAKLNFIGTT
                     A"
     gene            complement(98279..99724)
                     /locus_tag="PLU15_00360"
     CDS             complement(98279..99724)
                     /locus_tag="PLU15_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO29405.1"
                     /translation="MRVLEIALDRACATGAEYADVRALHQTEERLSVRNGVVERATSG
                     VSHGIGVRALVDGAWGFAASCVVSESEAARVAAEAVEIARASAMAKKCDVVLAPLDPV
                     VVEWESPCAIDPWSVPRAQKIDLLREACSRAMSGGSDVRYASASMNAIRDDKAYANSE
                     GARIRQRTTVTSAGIKAIALGNGEMQVRSYPGTFEGNVRQAGYEWIEEMDIAGHAAEI
                     GKQAQALLAAQQCPQIKTTLILGTNQLALQIHESCGHPTELDRVYGYEASYAGTSFLD
                     PGKLGDFRYGSDLVNIYADSTIPGGLGTFAYDDEGVRSCRTDLVRNGMFEAYLMSRET
                     AARLGKTSNGCMRASGWDRMPIIRMTNICLEPGNETLESLIRGTDDGIFMDGIKSWSI
                     DDRRLNFQFGSEIGWRIRNGQLAEMVKNPTYTGITPEFWNSCDGIAGSDEWVLWGFPN
                     CGKGEPSQVMFVGHGTAPARFRNVKVGVGRW"
     gene            complement(99729..100619)
                     /locus_tag="PLU15_00365"
     CDS             complement(99729..100619)
                     /locus_tag="PLU15_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO29406.1"
                     /translation="MKSWRLGSYKSILADLSLVGICVIWGTSFAIVKTVVGDTNPATF
                     IAARFVLATVLLGLVAGRRLRGLDWEYCKAGFALAIPLMLGFLTQTCGLQGTTASKAG
                     FITGMHVVFTLFLEQKVLKNRIMPYQITGVGVAVAGLGLLTLDRLSPPTYGDILILVC
                     AFCFAVHIILLARYSPTMDGLLLSFTQMAWAAVFSVLAAGSDIVQIFQFDKDLVLSVA
                     YLGATGTALAYLVQTTAQKYTPPTRAALILQTEPIFTSIFARLWLGERLSVQQAIGAI
                     LILAGILMCQVGDIKRSREV"
     assembly_gap    100908..101007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(101038..101976)
                     /locus_tag="PLU15_00370"
     CDS             complement(101038..101976)
                     /locus_tag="PLU15_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29407.1"
                     /translation="MGRAIAGEPRQDAEERVRRHILGQLHSHEIDRFELIRSKDRVWV
                     FRCSFDGSPTVIKYFEKDDDLREIHNYQLLHRLGIPTMDVLEYAQRCLVLEDIAESKT
                     WRLGIPDDLRDEQIAGRLAEWYFHFHEKGINIPELPELYCETDCLTIANLNRLSERDP
                     SLKDITCYLQNHVGLFQKELAGLVRTLNYNDFYWTNMLVRKDHTAAMMFDYNYLGQGY
                     RGNDISNVKSSLSVEAGTAFEDQYDMLYHGKYGVSRECERQNEERITSIVTPLITVIL
                     AYQKPDFPAWGEEARQLVLSPQYLEHVKSFVDSCDK"
     gene            102537..103130
                     /locus_tag="PLU15_00375"
     CDS             102537..103130
                     /locus_tag="PLU15_00375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO29408.1"
                     /translation="MVITDGRVILRDFVREDIEDRIRWETVETEWLLWDAPWELEEEE
                     PFSPDAYRDKMLRQLSTEKDPSRMRRSFQICINNGTRTHIGWCNSYNIDANYDYTDED
                     GYCTIGIDIPEQSARRQGYATAAWKLFIEYLLSNGVEDIYTQTWSGNERVLGLIRKMG
                     FEQCARQVNYRMVRGQSYDGLTFKLNISSFRKLVYGK"
     gene            complement(103530..104513)
                     /gene="guaA"
                     /locus_tag="PLU15_00380"
                     /pseudo
     CDS             complement(103530..104513)
                     /gene="guaA"
                     /locus_tag="PLU15_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     gene            complement(104535..105086)
                     /gene="hpt"
                     /locus_tag="PLU15_00385"
     CDS             complement(104535..105086)
                     /gene="hpt"
                     /locus_tag="PLU15_00385"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOO29409.1"
                     /translation="MHIIEDIQEVLISEEQIGSKVAELGAQISKEYEGKDLLLVCILK
                     GALVFMSDLMRCISIPVSIDTMAVTSYGASTVSSGVVKIIKDLDVPIEGRHVLIVEDI
                     VDTGLTLSYLVSTLKNRKPASVKVCTLLDKPDRRQASIVPDYNGFVIPDKFVVGYGLD
                     YMEKYRNLPFVGVPKDYVWNEEH"
     gene            complement(105201..105704)
                     /locus_tag="PLU15_00390"
     CDS             complement(105201..105704)
                     /locus_tag="PLU15_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO29410.1"
                     /translation="MLKDVEKDGIVKWEDVMASKGCPSSSRAEKGPVAVIECLQEIPC
                     NPCETGCPFGAISVGDRITNLPVLNEEKCRGCGNCVPVCPGLAIFIEDLSGSGDEAKV
                     TLPYEYLPLPERGAVVKATDRAGREICDATVVDVRKPAAFNKTAVITISVPKEFVHEA
                     RGIAFLR"
     gene            complement(105710..106450)
                     /locus_tag="PLU15_00395"
     CDS             complement(105710..106450)
                     /locus_tag="PLU15_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOO29411.1"
                     /translation="MRIDVSLVPGEIAAKDVSGSVVVIIDVLRATSSIATALASGCEE
                     VIPAESIEEAITLANGYKRSDYVLGGERRGVPVEGFDLGNSPQEYDGPRVAGKKVIMA
                     TTNGTSAINKYGIAREIFTACFLNADAVIGACEAYDDCDLVLVCAGQDGRFCMEDTLC
                     AGLMATRIAHDKGAEMSDSAKAASLLYQQRKGSIEDELLRCDHGAYLVGIGYEQDVRY
                     CSRNSILDVVPKVYDRRVIRRARGGPEE"
     gene            complement(106464..107363)
                     /locus_tag="PLU15_00400"
     CDS             complement(106464..107363)
                     /locus_tag="PLU15_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOO29412.1"
                     /translation="MSEVGERNVRQNANAGRSNTGRIWATIVIVVVAACCGLFLYFTY
                     LPAENGLFNILILGEDRNYSRNGDVLSAPGRPDTILLLMVPKSSRGAALLSIPRDTLV
                     RFPNGGVRRINSSVPEGGVDLARELVSDLVGLPVHRHVVVDFGGFVELVDALGGVEVA
                     VDKRMKYEDKAGGYSIDLEPGVYHMDGEMALSFVRYRQDGLGDISRTARQQEFVKAFI
                     REAGSFRAIPSYPNMLRVAQKCADTDMTVKEVAAVAWRLRRLDSDTINSATLPGYFKG
                     AYWDPDTQGIEALVRSLQDSAQK"
     gene            complement(107440..108477)
                     /gene="tsaD"
                     /locus_tag="PLU15_00405"
     CDS             complement(107440..108477)
                     /gene="tsaD"
                     /locus_tag="PLU15_00405"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOO29413.1"
                     /translation="MREDGVVLGIETSCDETAAAVVRGGREILSSVVASQIEVHRRYG
                     GVVPEIASRRHVEAIIPVIDEALAQAGTDLAEIDAVAATHGPGLVGALLVGLSAAKGI
                     SYAAGKPLICVNHIEGHIYANFLAHPQLSPPVLCLTVSGGHTELIAMRDHGRYEVLGR
                     TRDDAAGEAFDKVARVIGLPYPGGPALEQLAKSGDPNLISFPRAEVEGSPLDFSFSGL
                     KTAVINYIHTAEQRGEDLDYADVAAGFQKAAVDELVSRVDRAVDFTGMSVVAVSGGVA
                     ANQALRRALTALAGKSNIEVYYPPLNLCTDNAAMIASAGYYRHVAGEVSSLSANAEPG
                     ALLSSLACFDG"
     gene            complement(108470..108970)
                     /gene="rimI"
                     /locus_tag="PLU15_00410"
     CDS             complement(108470..108970)
                     /gene="rimI"
                     /locus_tag="PLU15_00410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOO29414.1"
                     /translation="MDLIPSPMNLDDLDRVLDIERLSFSLPWSRHAFVDELLMNDQAV
                     YMVVRDSDGVAMGYGGMWLILDEAHITNVAVHPEYRRRGVGRAIMVALMETAVLRGAR
                     RMTLETRLSNVPAQELYRSLGFVAAGVRPRYYLDDHEDALVMWLEDLPGRLAMMDGEG
                     GAPEDA"
     gene            complement(108939..109682)
                     /gene="tsaB"
                     /locus_tag="PLU15_00415"
     CDS             complement(108939..109682)
                     /gene="tsaB"
                     /locus_tag="PLU15_00415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOO29415.1"
                     /translation="MLILAVDTSTMVGGVALINDGALLGEHVLNVRATHSERLMSSVD
                     ATLTGANIAPGDLDAIACGVGPGSFTGVRIGVSAAKGMSYALGIPLVGVPTLDALAYG
                     RSPGFSEVWSMIDARHGRCYVACYKPSDSFGAAKRILDYSVLDVSQIVDSAGADGDRV
                     LFVGDGALAYSDSLLSAFPGRGVVPPAAFGILRPAQVGMLGMEMLEHGEGGDPAHVLP
                     MYLRPTEAEVKLSRKEMEKDGSDSVSHES"
     gene            complement(109676..110155)
                     /gene="tsaE"
                     /locus_tag="PLU15_00420"
     CDS             complement(109676..110155)
                     /gene="tsaE"
                     /locus_tag="PLU15_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOO29416.1"
                     /translation="MNKGENLKFMSNSPADTAALGCRLGRLLGPGDIVCLEGGLGAGK
                     TCLARGIAKGLGVDETSVSSPTFVLAQEYRGRVRVYHLDLYRLSSSDEVEEAGLDEYI
                     WGDGVAIIEWPGVYVPLREHDRLMVEITQEPVSLRRELVLTPYGQRYRQLLEEMRPC"
     gene            complement(110235..111260)
                     /locus_tag="PLU15_00425"
     CDS             complement(110235..111260)
                     /locus_tag="PLU15_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOO29417.1"
                     /translation="MSAPLVQVTRGELVESLHRGHIAVVDCRGNVLWQAGDPMYVTYF
                     RSSAKPFQVLPIIESGAVDRFGITERELAVMAASHHGEDCHVEAVMSILGKIGLDESC
                     LKCGVHKPTNPTQAARLAREGIPVTPVFNNCSGKHAGMLAYATHKGYPIDNYVDKESL
                     VQIDMWKAVAEICGLLPEKVVRGVDGCGVVVFGMPIANMAYAYARLACPDMLPERLRE
                     PARRVTSAMVEYPEMIGGTLDFGSNLMKNSKGAVFAKGGAEGLFCVGVPKRGIGIAVK
                     IEDGGSRGLQTAVMETLRLLDVVPQETFAEPQTSIVAFVRNCREDVVGEISPCFELAE
                     GNSRLSV"
     gene            complement(111274..111999)
                     /locus_tag="PLU15_00430"
     CDS             complement(111274..111999)
                     /locus_tag="PLU15_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOO29418.1"
                     /translation="MPPLILISSDFESGTNGKIAVGVEYAKAIYRSGGMPLILAPPMG
                     CADSNRVAHGILSDACGLLLTGGDDVDPRFYGESHAHPKIGAINPVRDEFEIALSKAA
                     LKLDIPVLGICRGAQILNVAAGGGLIQDIASCVDGAHAHRVSAPRWHPTHRVMLARNS
                     ILFGIFGEETIWVNSFHHQAVGPVAPGFSVTGVADDGVREAIEKPGSRFVMGVQWHPE
                     GLAERDAQSLRIFTEFVNAVRGV"
     gene            complement(112064..112414)
                     /locus_tag="PLU15_00435"
     CDS             complement(112064..112414)
                     /locus_tag="PLU15_00435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOO29419.1"
                     /translation="MDVKRGDVFYADLNPVVGSEQGGLRPVLVIQNDIGNYYSPTTIV
                     AALTSRIKRAKLPTHVELPAGQGYLAVDSVVLLEQLRTIDKTRLHDRICTLDEEIMKR
                     VNRAAEISLGLLEI"
     gene            complement(112417..112692)
                     /locus_tag="PLU15_00440"
     CDS             complement(112417..112692)
                     /locus_tag="PLU15_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOO29420.1"
                     /translation="MTLLRKIVVSMPAGLLEELDVCARQSGRNRSEVVREAMRSYLSQ
                     HARSDLREKLKHGYIEMASINLELARECAAAEDEAFAVSERFLAGGD"
     gene            complement(112931..114001)
                     /gene="alr"
                     /locus_tag="PLU15_00445"
     CDS             complement(112931..114001)
                     /gene="alr"
                     /locus_tag="PLU15_00445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO29421.1"
                     /translation="MRQIKSTLPKDTQILAVVKADGYGHGAAPVAIRALEEGVSFLGV
                     ALVEEGVALRRAGIAAPILFMEDLFPWQALDLVRYGLTATIASYESAEMLSEAAMALG
                     KKAKAHVKVDTGMGRLGLPPNEIANLVRRVAELPGIEIEGIFTHLACAECNSEMTGRQ
                     LEQFDDITEKLRREGHEIPIRHVANSAAMLSLPEAAYDMVRVGGIMYGLSPLGTVAPE
                     KPWVPALSFHTRVSYVKLVGPGTPVSYGATYVTSGREIIATLPVGYADGYPRALSNVG
                     EVLIGGERCPVVGRVCMDQTMVSLPADSDVRVGDEVTLIGAQGGDRIFAEELAKAAGT
                     IGYEIVCAISKRVPRVYVAGDE"
     gene            complement(114024..115715)
                     /locus_tag="PLU15_00450"
     CDS             complement(114024..115715)
                     /locus_tag="PLU15_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOO29422.1"
                     /translation="MVATRVATPDESRRIDRDAIELYKIDGIVLMENAGAAVARVAVE
                     IGAERSGWSAGRPSVCIFAGKGNNGGDGFVAARHLVGCGVDVSVYVTGSLDGISGDAR
                     TNLDILRNMGVDVVGLTCEEAWSAAADDLAVADVAIDAILGTGAKGAPCGEPARAISM
                     MSKSPVPIVAVDVPSGVDAGSGAVAGACVTAHTTVTFGLPKTGLLMYPGAAHVGYLLV
                     DRIGLPPALTDSPKIKTCIVCPDDVSAALPQRRPDAHKGDCGRVLVVAGSRGMVGAAA
                     LASMAALRAGAGLVYLAAPEGVAAQACAGLTEVIVRPLPETSSGSIAGIAVDRVLDEA
                     AKCDAVVVGPGLTCSDDSRLIVEALIAEGRTPMVIDADALNLLSGRVDTLQRASAPVV
                     LTPHPGEMSRLVEAGVEEINADRLEWARRVAHRSGAVVALKGARTVTATPDGIAYLNL
                     TGNSGMATAGSGDVLAGVIGSLMAQGVPPQWAAVSGVYLHGLAGDYAAREVGLHGMVA
                     GDILLRIPKAYHDIVEESAGLWDMMSVRSAQYKKKVVNPAARGLSARNEANVGRS"
     gene            complement(115684..116094)
                     /gene="acpS"
                     /locus_tag="PLU15_00455"
     CDS             complement(115684..116094)
                     /gene="acpS"
                     /locus_tag="PLU15_00455"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOO29423.1"
                     /translation="MCCQRVLGIGVDIIEIRRFHRIAAVAPQGVAQRLYTAEELSDGR
                     IGRPEYLASRFAAKEAVMKSLGCGMDSIAFTDIEVFQDEYGQPHVRLTGSARARADSI
                     GARTVMISLSHSDEMACAFAVSVGGGCNEGCDSR"
     gene            complement(116126..117505)
                     /gene="glmM"
                     /locus_tag="PLU15_00460"
     CDS             complement(116126..117505)
                     /gene="glmM"
                     /locus_tag="PLU15_00460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO29424.1"
                     /translation="MGKLFGTDGVREVANTGLTPELAFRLGRAGASSVLSRSGARPTI
                     IVGGDTRRSTGMLESALSAGINSAGVDVLNVGVIPTPAVAYLVVKFGADAGIVVSASH
                     NPAEYNGIKFFGPDGFKFPDEVEDIIEKRALAGGSGEMGSVLWSEDDGLPRPSGSGLG
                     MRKEAPDAWERYVEHAVESASCRLDGLRVVLDCANGASCLTTPAAFRELGAEIIVINA
                     SPDGDNINCACGSTHPDELAAVVVERGADMGFAHDGDADRVIAVDADGRIVDGDQIMA
                     LSAIHRHSRGGLPGRAIVATQYSNLGLTHAMESIGCSVLTADAGDRYVLQMMLEKNLV
                     IGGEQSGHVIFLEKATTGDGLITAIEVAGIAVETGKPLSELAAVMTKYPQIMVNVRVS
                     RKDDYYHSDTIAEAVKAAAAELGSSGRLLVRPSGTEPVVRVMGEGKDEAKVDRIVQSL
                     AEIIQSELC"
     gene            complement(117555..118463)
                     /locus_tag="PLU15_00465"
     CDS             complement(117555..118463)
                     /locus_tag="PLU15_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOO29425.1"
                     /translation="MKPVSPEAKDTSIKVLAVVLAIAIWAVAISDKQRTSPSPKIELV
                     VKAPVSLRNLPDDLIVTDLPETVVIRLRGPKDLESTALESTVAVVDLEGAKPGQNLFP
                     IEVVAPNGFELVRASRAVADLTLEKRAVVSLPVDIAIVAAPHFNVELEHDLTGALHLF
                     FAQKVPDDFRSEAESLAEGGASLGQGLSEGGTEPEKAADEEMGASAGSASGLHGGRLE
                     GSESELVDGLRSSVSVSVSPAEVSIEGPETAVSRVARAVAMVAVGHESTVVVPVLALD
                     IHGQTVSDVIVYPETVTVDFYSKARW"
     gene            complement(118460..119290)
                     /gene="cdaA"
                     /locus_tag="PLU15_00470"
     CDS             complement(118460..119290)
                     /gene="cdaA"
                     /locus_tag="PLU15_00470"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOO29426.1"
                     /translation="MNLLGFDFSNITLMQALTTIADIAIVAFLMYKVISFLKGTQAVS
                     LIKGLVVLFAANAISEYLKLEALHWLLRQATTMAIVGIPIVFQPEFRRVLEQLGRGRL
                     FGNPMKSLDPEEKKKVLSEVARAVEAMSKEYIGALIVLEGETGLEDIAASGVKLDSTV
                     SAELLINVFTPNTPLHDGAVILRGNRILAAACILPLSDRHVTGRKLGTRHRAALGLSE
                     RTDAVAVVVSEETGAISLANDGEIVRGLRPEELRDRLESLFDHNTKDTVIRNLRSNGR
                     "
     gene            complement(119403..120194)
                     /locus_tag="PLU15_00475"
     CDS             complement(119403..120194)
                     /locus_tag="PLU15_00475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOO29427.1"
                     /translation="MRLVAMDLDGTLLRSDKSISDAAIEAIREAHSRGVAFAVATGRM
                     FQTSQPVVDGLLDELPIIAYNGALIKYSNCGLTLLRLPIAARGGERVVRFMWEWGIVF
                     QAYIDDQLWVARVAPETVAYSEKYGAPINVMEDIEEFVNREAFKYLVLDDEERIADVR
                     KAIDGVLDPGLRTMLSSPGMIEIVRSDVSKGRALSHLASSMGISMDNVMAIGDSENDI
                     DMLRAAGIGVAMGNAGDDVKAEADYIVASNDDDGVVEALERFALL"
     gene            complement(120198..120593)
                     /locus_tag="PLU15_00480"
     CDS             complement(120198..120593)
                     /locus_tag="PLU15_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOO29428.1"
                     /translation="MAYHSNGQVGMPEWVIKWLINALALLIVSYVPYRGGTLLEIDGA
                     GAALIAAMVLGIVNTFIRPIFMMFSLPLRILSLGLFTLVINALMLCMGSWLMGNAFNI
                     PGFWAAIIASLLLSLVSSALNHLIRGDSR"
     gene            complement(120657..120848)
                     /locus_tag="PLU15_00485"
     CDS             complement(120657..120848)
                     /locus_tag="PLU15_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOO29429.1"
                     /translation="MQRRLYRSVRDRMLGGVCGGLANYLNVDPTLIRLLWVAFTLFSQ
                     APGLLLYVIAWIIVPEEPI"
     gene            complement(120992..121363)
                     /locus_tag="PLU15_00490"
     CDS             complement(120992..121363)
                     /locus_tag="PLU15_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29430.1"
                     /translation="MDQERKQVLEMVRDGKLTVDEAQQILDAMTYEEERARPAASSRR
                     NPRFLRIRVKDEDGSKVNLNIPISLAKVMWRFIPREAMNELSNQNIDLETILDAVHEG
                     AEGQLIHVKDEDGDEVEISVV"
     gene            complement(121419..122483)
                     /locus_tag="PLU15_00495"
     CDS             complement(121419..122483)
                     /locus_tag="PLU15_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOO29431.1"
                     /translation="MGEERKIVLNLLSEGKITVEEAEQLLAALEGEVESVDEADSVRV
                     ETISEGDLHGRLSKVFAEMVEAGQELPGRLSRAVESAFGGFGWSFRGVRGEKSFEEEI
                     CGPEINAIDFAANNGSIKLAVWEKPGFRVVVRATVVDADSRDEADRRLESVARLVVND
                     GKVSIDCEDKSILDSMSIEAYFPKDRKYDVFAHTRNGSITLDRLNGGHVEVCSSNGRI
                     ALDSIKVSTLAAETANGAVTASGHLGDGSIETSNGSVTAALAYESGGHLNVRTSNGSI
                     RIKVLNSSDVSYEVNAETKNGATRVLDEGAAIRSERRSTGGQVKKITVSAGDSALDDS
                     AKVTVDARAMNGSITVESCR"
     gene            complement(122515..122892)
                     /locus_tag="PLU15_00500"
     CDS             complement(122515..122892)
                     /locus_tag="PLU15_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOO29432.1"
                     /translation="MARREALGRCPVCDGILDVTRLTCTDCETVIEGRFDTCKFCQLS
                     SEQKNFVEVFIKNRGNIKEVEKDLGISYPTVRNRLDAVIAALGYAVDDSEDDVATRRK
                     EIIESLAKGEITADDAVRQLKGK"
     gene            complement(123032..123796)
                     /locus_tag="PLU15_00505"
     CDS             complement(123032..123796)
                     /locus_tag="PLU15_00505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOO29433.1"
                     /translation="MEKDDGSITTSSCASPDRSAISVSSIGTSATEASAIIASASVAT
                     RIGIVSIEAGESGLSRVVLPRIDMETLKNLPTSPRIHPLFIDASVTLAQIGIDFPMCD
                     HSSICQMAASNSVPVYGSVSWDGVPPSEIVSAACRDIKLFFEGELDPGKLWFDYPLKL
                     PAGSDFAREVMVGMTRIAPGRVTAYGALAAAAGRPRAARATGAIVGANPLPLVVPCHR
                     VVGVGGRLTGYGGGLPLKVALLAMEQTIINRTGVLG"
     gene            complement(123901..125751)
                     /gene="uvrC"
                     /locus_tag="PLU15_00510"
     CDS             complement(123901..125751)
                     /gene="uvrC"
                     /locus_tag="PLU15_00510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOO29434.1"
                     /translation="MALDDTLAVLPTSPGVYLMKDADGEVIYVGKAINLRNRVRSYFQ
                     TSSDHSPKVVMMVEHIADLDYIVTASELEALILECNLIKKYRPRYNVRLRDDKTYPFI
                     KVTTGEPYPGVYLTRRVVRDGSRYFGPYTDVSATRETLRLLKRVFPLRQCSKKIDPDS
                     PAPRPCLNYQIKRCLGPCAGQIDVEAYGEVVDEVVMFLEGRQDDLARSLKRRMVAAAD
                     ALNYEQAAVFRDQLQAVETVVQKQRIVSESGADQDVIAIARDQWGACAQILFVRGGKL
                     VGNDHFMLGGTDGADDAETLSSLVKEYYSQAEAGSLPREVLLESEIEDADIVAAWLSD
                     LRGRAVSVKTPKRGEKRGLVELASANAAEFLERKRAAEQREARARMDALAELSIHLRL
                     SKSPSRIECYDISTLQGTEAVGSMVVFEAGLPAKSQYRRFRIRQVEGQDDYAMMAEVI
                     RRRLVRAIGDDPDPKFARLPDLIVVDGGRGQLNAAADVLDSLGRGDIPLISLAEREEE
                     IYLREQPDPLILPRDSAALHMIQYIRDEAHRYALAYHRNLRGKRSISSELTDLPGIGK
                     KRTVALMKEFGSLEALTDATVDEIAATPGMNRVVAMTVYEHLHPDRGVDG"
     gene            complement(125738..128614)
                     /gene="uvrA"
                     /locus_tag="PLU15_00515"
     CDS             complement(125738..128614)
                     /gene="uvrA"
                     /locus_tag="PLU15_00515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOO29435.1"
                     /translation="MSVEKIIIRGAREHNLKNIDLDLPRDRLVVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMNKPALDYIEGLSPAISIDQKAQGRNPRSTVAT
                     VTEIYDYMRLLYARIGIPHCPNCGREIAQQTVQQIVDRIMAMPEGSRIQVLGPVIRGK
                     KGEHRKALDDLKRDGYIRVRVDGEVMELSEEISLERYKMHSIEAVVDRLAVRPGIETR
                     LADSLETALKLGDGVVIIDVVGGKELVFSEKLACPDCGIGFGELEPRLFSFNSPYGAC
                     PVCSGLGVKIDFDPKLIFDMEHSIEDGAILPYNGNGVIADYYMGRVNQVATEKGIDTS
                     VPMSELPESAMEMIMYGCGSEKFRFRYVNSRGRTKYWESRFEGVIPNLQRRYKETTSD
                     YIRENLERFMSASPCTKCNGARLKPEALAVTVGGKSIAEVTSMSTVKALEFFEELGRG
                     LTERESLIARQILKEIGARLGFLTNVGLDYLTLHREAGTLSGGESQRIRLATQIGSSL
                     VGVLYILDEPSIGLHQRDNARLIDTLKDLRDLGNTVIVVEHDEEMIRSADYIVDMGPG
                     AGAHGGQVVAAGTVEEIEANPASITGQYLSGVRRIEVPAARRLPNGNALVIKGAREHN
                     LKNIDVTIPMGVFVCVTGVSGSGKSTLINEILYARLAHDLHRAMTRPGAHDEILGIEH
                     LDKVIGIDQSPIGRTPRSNPATYTGVFTHIREVFAKTPEARARGYKPGRFSFNVKGGR
                     CEACRGDGIIKIEMHFLPDVYVPCEVCHGARYNRETLEVKYKSKSISDVLEMTVEEAL
                     EFFANIPAIKRKLQTIYDVGLGYIKLGQPATTLSGGEAQRVKLATELSRRTNGRTLYV
                     LDEPTTGLHFADVQKLLDVLQRLVDGGDTVVVIEHNIDVIKTADYIIDLGPEGGDKGG
                     RVIAAGTPEEVAATPGSYTGEYLVKIAGVMPAQRELEAMTGGTR"
     gene            complement(128611..130650)
                     /gene="uvrB"
                     /locus_tag="PLU15_00520"
     CDS             complement(128611..130650)
                     /gene="uvrB"
                     /locus_tag="PLU15_00520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOO29436.1"
                     /translation="MPQFQLKSGFAPAGDQPRAIAQLVAGVKAGMRGQVLLGVTGSGK
                     TFTMANVIQELGRPTLVIAHNKTLAAQLASEFREFFPNNAVHYFVSYYDYYQPEAYIP
                     QSDTYIEKDAQINDEIDRMRHAATAALVERRDVIIVASVSCIYGLGMPEEYLNQAVVV
                     NAGEYLDRDRLLRKLVGIQYSRNDIGITRGTFRVRGDVVEIIPASGEEAIRIEFFGDE
                     VDRISDIDLITGEVLGLRESAFIYPATHYVTSKERLDAAIEAIGVELAERLKELKRDG
                     KLLEAQRLEQRTMFDIEMLEEVGYCSGIENYSRHLAGREAGSAPATLLDYFPDDYLMF
                     IDESHVTVPQIRAMYNGDRSRKESLVQYGFRLPSAFDNRPLTFSEFEERVNQVIFVSA
                     TPADYERTVSSQVVEQIIRPTGLVDPAVTVKPVRGQVDDLIGEIRRTVDAGYRVLVTT
                     LTKRMAEDLTDYLREIGIKVRYLHSEIQTLERVEILRGLRLGDFDVLVGINLLREGLD
                     LPEVGLVAILDADKEGFLRSDTSLIQTIGRAARNVDGRVIMYADQITGSMREAIDETE
                     RRRSAQIQFNREHGITPESIKKEVRDLSEIQRVADRSVGSSAACGGQSGVRGQGGVAA
                     EFGCADVESMDERELARYIKALEDEMKKAAGLLEFERAAELRDTIRDLRRRFVEL"
     gene            complement(131055..132713)
                     /locus_tag="PLU15_00525"
     CDS             complement(131055..132713)
                     /locus_tag="PLU15_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOO29437.1"
                     /translation="MKRKPYSLWVAVLACLAMTLTGCGKEVKVVHEDGQTIYDYSRKS
                     EKLSSELTKALLESGVIDKSVAANGRQEKIMRLSSKEDTYLRWTITSYEAGASSASYQ
                     SLSAAVSRAARKANARVIQEIADGDSSAGDCAIFILGFSDRSLQGLATYLEIMQVVVP
                     WSRSLGQTVAPTRNEQYLESIQGLDVSETFSIEETASGRDEALLDLEEMPVGAGSVLS
                     KPQENVVPRYTSKEPPLSSSERKTLNGSRPRLAIVIDDLGSGVAATDELLSIDVPLTV
                     AVIPQGKYAAKEAKAASERGFAVLLHQPMEPLDPSKRPGKGGILMGMDEKEIRSVISS
                     NLALVPNAIGVNNHMGSRVTEDPESVRIILDEVFAHGLFFFDSRTSSNSVVPRVAREM
                     GLPLLENSRFLDHIDTEEYVIESIRIVARLAMERGSAAAIGHVRPATVRAIQKMLPEL
                     DAAGVELVTLDKLMPTPSRTAEPKPSLPAVPATVKEAAPAAPVQLPVEADKEEAGIEA
                     LPAVEPQAFSEPEPRQELEPEPGECGETNTGQTVDDPGVDLSEP"
     gene            complement(132710..134002)
                     /locus_tag="PLU15_00530"
     CDS             complement(132710..134002)
                     /locus_tag="PLU15_00530"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOO29438.1"
                     /translation="MQEFPFGNVFRIILESQIALFRYPSSMIWFWIVMSIVYFQYRRM
                     SALETQIFGRPRRSAIVRTMCSLGEGMVGGILGSFLIILFGASAESLGLGAIWLIALA
                     LASIDIRFICFSYAGGIVSLLNLAFGWPDVDVTSIMALVGVLHLVESALILLSGHRDA
                     APVIVSGPDNKPAGGFYMSKYWPVPIAILWITYSLATTGTPEGGIAMPDWWPLLKSAA
                     PPSPDMRADYWVLAITVAMGYGDATYSRLPRQKTKATAGMSALFSLALLGLTLLSERY
                     PWVRWVAALFSPIGHEFVIGMSQKAELEGEPLFTTPAQGIRVLDVGPGSPCDQAGIKP
                     GDVIEAVNCQPVNSFADFAEALRSVGYATILDIRSGLPGSKGENRTVQIRGVPDGLGL
                     LLVPTEGDYPKMDSGRAGLIAIIADRIKRRSSRRRRRR"
     gene            complement(134018..135334)
                     /locus_tag="PLU15_00535"
     CDS             complement(134018..135334)
                     /locus_tag="PLU15_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO29439.1"
                     /translation="MSSYKKAQRSAWLVLTVAAAILVCTVLAPSAFGVPSPQDEEAAP
                     KFKDIHTAIEAMYLVKAQFYKPVSVAELMKAYFAKGTVPGMLEVLNDPYTRYMTPQDF
                     QALKDDSAGIFGGIGILVGIRDDKITIISPITNTPGMRAGLMPGDHIVEIDGVSTEGM
                     VLDRAVSMMRGEPGTVVRLLIERRYPAEKFEVEIVRDIIDAPATRGYIFDPDAGIGYI
                     ELRTFSQKAGQDIEEEVERLMDEGMRGLILDLRYNGGGLVTQAVEVASKFMPPGPVMH
                     VRGRDGQKKSLYTVGRRVSQIPLVVLVNEYTASASEIVSGALQDTGVATIVGVPTFGK
                     GLVQTLFPLSDGSGLAVTTQIYLTAGGREITPETPIMPDVKVEALTPEELEKAMNDQA
                     RNAERTGPVTAEDLMRIDPDLDPQFRRALEILRYQIEKDEPVKSAA"
     gene            complement(135415..136617)
                     /locus_tag="PLU15_00540"
     CDS             complement(135415..136617)
                     /locus_tag="PLU15_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO29440.1"
                     /translation="MGIIKAARAVLSLVLIMTLAFGFLTDVAVASSQQSDISSKRTEL
                     NSLQSKIEQIRQTLSRKQKEEKDALRALQKTEEEILAIETRISQTEKKLKDAEQQLVQ
                     VQTELKEAERQLEIATAQYEASVQSLEDRLVFIYKLGPGTLLELLFGASSFSDFVSRY
                     DYLGAIAKQDADIYGEIRAEKESLEEQKEGICARKAKLEETRREIALLSSALEDERRS
                     IEPVLAQRSQYASRVSAERERWERELAEEERASRELERIIREMQARTGGTISWTGKFI
                     WPVKFTRISDVFGWRVHPIYKTRRFHSGMDLAASTGTPVKAAADGKVLLAGWVNGYGN
                     TVILDHGNGLSTLYGHASVITTKAGNVVLQGDIICKVGSTGVSTGPHLHFEVRQDGTP
                     QDPMRWLP"
     gene            complement(136657..137535)
                     /gene="ftsX"
                     /locus_tag="PLU15_00545"
     CDS             complement(136657..137535)
                     /gene="ftsX"
                     /locus_tag="PLU15_00545"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOO29441.1"
                     /translation="MKLRTVKSLSGQAFRSLKKTPSVTSIIVVMACMLVLSSFMLVAV
                     NLTRIGNEFQAGVQIRAFLSPDMPPDAVDRTHAAIQAIPGVASVKYVGKDEALQRLGK
                     QLGENNFFLGDLPRNPLPESFDISLLRAADADRIAAAVLELDEVSDVRYAPKLVDRLV
                     GVLRIFWVVTGIIAVFVLVGASLIVSNTIKLTVFARRKEIEIMKLVGATDAFILFPFV
                     LEGIIIGLLGAGAAAVIVGAGYQGLAKWISSVAPFMPIVTDSGHLGLAIVIMLCFGAA
                     VGLVGSNISTKRYLKV"
     gene            complement(137525..138211)
                     /gene="ftsE"
                     /locus_tag="PLU15_00550"
     CDS             complement(137525..138211)
                     /gene="ftsE"
                     /locus_tag="PLU15_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOO29442.1"
                     /translation="MVNMYGVTKVFPGNVEALRGVNLNIDKGDFVFIVGPSGSGKSTL
                     LSLIYRGTVPTRGHVLVAGRNVARLRWRDVPMLRRRIGVIFQDYKLLPDRSVYDNVAF
                     ALEVIEASPKEIEKKVPQALSMVGLAHKASAMPEELAGGEQQRVAIARAIVNDPLILV
                     ADEPTGNLDPDTTLDIMDLLCDINVRGTTVVMATHNHVVVDKMRKRVVQLERGMIVRD
                     ERWGAYCHEA"
     gene            complement(138352..139296)
                     /locus_tag="PLU15_00555"
     CDS             complement(138352..139296)
                     /locus_tag="PLU15_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOO29443.1"
                     /translation="MTKMIATRVAYGEALAELGGINSDIVVLDADLSGSTKTAGFASK
                     YPERFIQMGIAEADMMGTAAGLAATGKTAFASTFAVFATGRAFDQVRQSIAAPGLNVK
                     ICATHAGLTVGEDGGSHQSIEDIALMRVLPNMTVIVPSDAVQTGWAVRIAAEQKGPFY
                     LRLGRASVPTLYDEDADFELGKAKTLREGQDVTIIACGVMVSRALEAADRLEERGIRA
                     RVLDMHTIKPLDEEAVRAAAVETGAIVTVEEHSVIGGLGGAVCETVCSRAPAPVIRVG
                     VADMFGKSGKPDELLEAYGLTVENISFAAKRAIEMKRA"
     gene            complement(139293..140147)
                     /locus_tag="PLU15_00560"
     CDS             complement(139293..140147)
                     /locus_tag="PLU15_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO29444.1"
                     /translation="MKRVVLSDPEELAAKACEIRKHVIRMVGAASSGHPGGSLSAADI
                     VTVLYFNKMRIRPDDPTWSGRDRFVLSKGHAAPVLYAALAEAGYFPVDDLITLRRIDS
                     YLQGHPDMNQTPGVDMSTGSLGQGLSAACGMAIAAKMDGADWRVYAVLGDGEIEEGQV
                     WEAAMTAVHRKLDNLTIFVDWNGLQIDGPIREVKSVDHIGERFAAFGWHVIEIDGHDI
                     PAIMAAIDEAQRTSGRPTAIVARTVKGKGVSFMENEAGWHGTAPKPEQVEAALAELEA
                     SRKGGVAQ"
     gene            complement(140153..142012)
                     /gene="csaB"
                     /locus_tag="PLU15_00565"
     CDS             complement(140153..142012)
                     /gene="csaB"
                     /locus_tag="PLU15_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOO29445.1"
                     /translation="MRVLVSGYHGMGNLGDEAVLAAFVQHLKEIDPKAECVALSGDPS
                     ETSAAHGIEAVPRASLGAIIREIRRSDIVISGGGSLLQDATGKKSVPYYLGIIALARM
                     MGRPVSVYAQGVGPLNRAFSRWLVKRTLPRASVITVRDEASARLLQEVGVVKPEVMLV
                     ADPAFGLVPCDPPDAMAVQAPNTVMFCLRPWPGLDGREAEYALAIDRAVSELGICPIF
                     LAFQPDRDLEVAHRIADQCRAGEKSPAVISCDMVPEEVLGLISRAEMVVGMRLHSLIF
                     AANAGVPAVAIDYDPKIRAHCQRVSEPYIVEVGASADQLYAAIAGAWASRDETRARLR
                     EIMPTIREQAKAAASISLSPILDKPVTERMKAQAEEGKDYMRASVLGSPVDCIGMDDA
                     VYILMERAALGLGAHVVTINPEMALAAKRDAELADVIRGADLVVPDGVGVVRGLRILG
                     YKPPGRVPGIELATNLMRSAAGGEMSFFLVGAAPGIAEAAAENMSKEFPGLKIAGTWH
                     GYFSDAEEEEVLRCIRDAAPNFVFVGMGAGKQEKWITKARQMAPCAVWIGVGGSFDVM
                     SGSVKRAPAAFQKLGLEWLYRLVSEPRRAKRMTALPAFVFAVMGEAFRKSRRS"
     gene            complement(142055..144106)
                     /locus_tag="PLU15_00570"
     CDS             complement(142055..144106)
                     /locus_tag="PLU15_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOO29446.1"
                     /translation="MGHRKPRSIGWLIALIVLASVSSAWIAWQRNRVEKANSVVELCM
                     DYSEIALYSRRVGINIEDCLRGLAEEGIVSVALEEDTLGSMETAGEIVIIRGSEALAL
                     GSNGSPYRDILLAVAEMEVFSPSDTIVIPCNVDAATFLANRLTLRTCDGPSISVIRAG
                     DATGYAVSLPVDEARKLNLGIRPSKTAQVQAAGLRVVVRFSNFPNVSEKWIDSVMTLS
                     AVGAIPDCVIFAGTEVLGYPRFVSATVCGLDRLGTVYGDIEFQNQAGGRQLARALDYK
                     TVRVHSISRAEVDRDLSVAAMRDRYARAVRERNIRVLYMRPMMKPIDERSLPEVNQSL
                     VAEVVSAITDQGFSLGPAAPFDQLDPGLLLTLLVVLGVAAGCVVLLDAIWDLPFAICI
                     GLVAVFAAGFAALWGLGYPLLARQSVALAAAIAFPSLAAFVLFCPDIPGKTRDCDTCD
                     DGALLANKDWLGSSGTKFLTASMLCLAGGLMLAACLTSRAFMVKGEQFLGVKAMHIFP
                     MLFVLVAYWKRYARRGNESLIASLARLNRSNILVWHVIVIAILGVAGLIYIARTGNTS
                     SVPVAVPQWEQKMRAALENIFPARPRTKEFLIGHPAFVLAGALVALGETGLLLPISIL
                     GLIGQISLTNTFAHIHTPISLTLARVIIGLGLGFAIGLVATVVYRAVVAQIRRAAGRE
                     G"
     gene            complement(144106..144831)
                     /locus_tag="PLU15_00575"
     CDS             complement(144106..144831)
                     /locus_tag="PLU15_00575"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOO29447.1"
                     /translation="MARRVSPRRIPRIAIIMLLLVAATEVAVPFYVEKKVESAVRSGF
                     ASAESISVAARAMPALKLLYGHFDRLSIDIEEAVSSGFVIRRISLDSSDARIPLGRFE
                     PGSSRWARALGNSQLEVIIDESDVNDYLGHRDDYLRAFGVRFHPGIAELAGRLFLGGF
                     SVGVESQGHFSIGDGVKLVYVVDKLIVEKSEVPAFIVDMFEDGIAIGIDLSDLPFDIE
                     LESVEVGEGMVRLLGRTSEGGSL"
     gene            complement(144833..145955)
                     /gene="prfB"
                     /locus_tag="PLU15_00580"
     CDS             complement(join(144833..145897,145899..145955))
                     /gene="prfB"
                     /locus_tag="PLU15_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076737.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOO29448.1"
                     /translation="MRAELEQPIELLSERIDEMGPIFDIPGKLERLAQCEARAAEPGV
                     WEDRDSAQSLMQKISRLQAEIASYDSIVSRFEDLKILAELADEEDDEDAIGECFEEIE
                     ALESAVDKVEISHLLSGPHDSANAIMSIHPGAGGTESQDWAEMLMRMYARWAERNGYT
                     VEVADLLPGEEAGIKSATLIMHGHNAYGYLKNEKGVHRLVRISPFDASARRHTSFASV
                     DVVPEMAEEDVEIDTADLKIDTYRSGGAGGQHVNKTDSAVRITHLPTGIVVQCQNERS
                     QHSNKETAMRVLRARLAELQREEKEKELSAMRGEQHEIAWGSQIRSYVFQPYTMVKDH
                     RTELETGNVDAVMDGEIDEFIEAQLRLSAASANRDAGSE"
     gene            complement(145978..148539)
                     /gene="secA"
                     /locus_tag="PLU15_00585"
     CDS             complement(145978..148539)
                     /gene="secA"
                     /locus_tag="PLU15_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOO29449.1"
                     /translation="MLNLFKRIFDVNAKELRDLGTRVDAVSALAPEAQRATDEQLRAR
                     TGEFKGRIERGEPLDSILPEAFATVREVARRTIGLYPYDVQVMGGIVLHEGKIAEMKT
                     GEGKTLVATMPTYLNALEDRGVHIVTVNDYLARRDAEWMGPIYNALGLTVGVIVHDQQ
                     GTARKDAYMADITYGTNNEFGFDYLRDNMVTSMDGRVQRGHNYAIVDEVDSILIDEAR
                     TPLIISGQAEESTEKYFVFAKIAQSLKEEEDYTVDEKAKTVSITESGVARVERWLGVS
                     NLYDEENIELTHHLNAALRAKALMRRDRDYIVKDNEVIIVDEFTGRLMFGRRYSDGLH
                     QAIEAKENVHVQRESQTLATITFQNYFRMYQKLAGMTGTAVTEENEFIKIYNLPVVQI
                     PTAKPMIREDLPDAVYKTERAKFQAVVDEICERHATGQPLLVGTVSIEKSELLSDMLS
                     KRGVPHQVLNAKNHEKEAEIIKLAGQPGAVTIATNMAGRGTDIVLGPGVPELGGLHVI
                     GTERHESRRIDNQLRGRSGRQGDPGSSRFYVSLEDDLMRLFGGDYISGMMERFGWTED
                     EAIEHPTLTKAIERAQQKVEGRNFEIRRQVLEYDDVMNKQREAIYARRAAILEGTDLR
                     DQAIELVGTMVEGLVRAYADEKLGHGEWNLRGLSMEIASILNVRSGPDLEAREFHSWT
                     ELHEAVLTIFMRAYEQKEAAVGAERMRELERAIMLYLIDSKWVDHLQAMDMLRDGIGL
                     RAYGQLDPLVEYKRESYMMFNDLLRSIGEDFVRYVFRVRIRIEGESESASPQDTGAAA
                     AAAGLRRPKYAVSTNRGTDSSTKAPARRDGKKIGRNDPCPCGSGKKYKKCCGAQK"
     gene            complement(148578..149714)
                     /locus_tag="PLU15_00590"
     CDS             complement(148578..149714)
                     /locus_tag="PLU15_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29450.1"
                     /translation="MDGLVALVFEGGRRQGEISGWVERVREELAISLCRKLASSGVFD
                     DVVLITDGPALADRAGAIDGVSVELDRCCDNYHFGARLVQALQARAAMGFVYMGGGSG
                     FFMTVPEIVNFAEMVKNNPEAIVANNLFSADMFGTASSDAIARLNLPSNDNSVPMIAG
                     AAGMDLVALPPSVGSVFDVDTPSDLLVLASVIPQLAPEETAVLDAIRSGPIEPAMSRL
                     AEAREVLGRDFAEVGLFGRVSPATTMDLNTCTRCRLRVFSEERNMRALGRDVPGGARS
                     LIGKHIEAVGPEAFFADLAWCCDAAFIDTRVLFFHMRADLSQEERFASDLFLYDRLAD
                     NGVARFVKAAAEAGLPVTLGGHSLVSGGVRLLAAAEHGGRCDVV"
     gene            complement(149807..150352)
                     /gene="raiA"
                     /locus_tag="PLU15_00595"
     CDS             complement(149807..150352)
                     /gene="raiA"
                     /locus_tag="PLU15_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOO29451.1"
                     /translation="MEITVVGRNIAVTDALRAYAEKKVDKLQKYFEREIVDAQVTMAV
                     ERGIHTADVTIQVDGLLLRGEERTGDMYASIDGAVDKIERQIRKHKTRINRKLRQDGN
                     RLVESAVGHGAAADASEPSELKVVRVKRFAMKPMSVEEAIMQMELLGHDFFVFMDDST
                     EDVNVVYKRKDGNYGLIEPEF"
     gene            complement(150494..153274)
                     /locus_tag="PLU15_00600"
     CDS             complement(150494..153274)
                     /locus_tag="PLU15_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOO29452.1"
                     /translation="MTPDTAKPWHAVVYGEALVELRTSRQSGLSDEEVRARLEEYGPN
                     MLAEPPRRTIFQQFLAQFKDFLVILLLISAGVSGVVGEVEDALVIIAIAIVNAVIGVI
                     QEQRAENALEALKKMASPKARVIRSGEPRVIDASTLVPGDIILIEAGDSVPADARLIE
                     SGMLKVDESSLTGESEPVELCASAVLDEDAPLGDRVNMVYMGSSVSYGRGVAVVVSTG
                     MSTEMGKIAALLQEGKDEPTPLQVKLEAVGKKLGIAAVLLCVGVFAAGVFRGEAAFDM
                     FLTAVSLAVAAIPEGLPAIVTIVLSIGVQRMAAKNAIIRHLPAVETLGSADVICSDKT
                     GTLTTNQMTVRRVYVPGRVLEVSGDGYSAEGAFIESGTEIDVSEDPSITRMLASAVLC
                     NDARVRSVEIETFGSEESLDDSPVRLEMIGDPTEGAIVVAGLKAGVDKVNLTQWAPRI
                     EELPFESERKRMTTIHRLPEAYLSCTKGAPDELLKVCSSVYDGTEIIPLTEALREEIR
                     RVNAEMASSALRVLAVAQREFDELPPKISVDTIENGLVFLGLIGMQDPPRAEAKTAVQ
                     ECFDAGITPVMITGDHKVTAVAVARDVGIMTPDTIAITGAELSQMSNEELDDAVDRVR
                     VYARVAPEHKVRIVDAWKRKGHIVAMTGDGVNDAPALKRADIGVAMGITGTDVAKSAA
                     DMIITDDNFATIVTAVREGRVVFDNIKKSVQYLVSCNVGEIIVIFAAIMLGLPRPLLP
                     IQILWLNLVTDGLPALALGVDPPAPDLMRRKPRRGNDDVLGHAAVSRLVVYGISVALL
                     GLSSFAMGLGWPGPLDTSAASLAEARTMCLLTMSFCQLVHAFNFRSELDSLFKVGMFT
                     NRRLVMAFVASAILQLAVVYMAPIRGFFDLVDVHGEKLVNSLALALIMLVVGEVTKFV
                     SREVSRRRGHEA"
     gene            complement(153756..154559)
                     /locus_tag="PLU15_00605"
     CDS             complement(153756..154559)
                     /locus_tag="PLU15_00605"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOO29453.1"
                     /translation="MIQLSRFRDFVDTVLFGGELRCASCGRIADRSGMCSQCKERIAY
                     VLPPVCSLCGTPLRLLGPKTRGEPASNPENQVCSGCATTRHYFERARAVAVYNGAARD
                     HIHELKYRARTELVAPLARMMAEVAIGDGMAGECQCVVPVPLHPEKAARRGFNQAQLL
                     AEDVGRLLGIPLEAKALQRQRSAGSQTFLDRRDRRANVIQAFLCPFPALVSQRNVLLV
                     DDVLTSGATADGCSRALLMAGAAKVYVLTFGVSVADERDWFEAPGSSRA"
     gene            complement(154563..156392)
                     /locus_tag="PLU15_00610"
     CDS             complement(154563..156392)
                     /locus_tag="PLU15_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO29454.1"
                     /translation="MAYAEAKWHHDLQRWADAMFHSYLIFSEPKVWAGFGRSPEFEWM
                     FLNTRSPTDLVVLTPPLPVAIAELVARELNASIPIDAPPERALKRGRRIAKSVLSAVG
                     YGGRSRLPRPMLLSASSFDSSVHGLDLPPSDVLKACIDELGGRALLDEEAKRALADAG
                     LCGLDRADHLLGAMVLDGNATILPGVGITSIGGFKCNRCGSRGPFLQLPCARCGRDIC
                     AVCSECRNMGIVRECTPIYLVNPPYVGTSIIGSVADKANMVMPHALTPAQVDAARALV
                     EWYETDESPEAIVWAVCGAGKTEVSYGLIAAALQAGKRVAYAAPRRDVVAELSPRIQS
                     AFPAARCGVFYGGSSERYFGADITLLTTHQAMRFYGVFDLVIIDEADAFPYYGSEALA
                     ASVDRSLAFGGRTVVLTATPSLGHIARIKAGTLPCFKISARHHGHPLPMPVVIEMGDK
                     PQEPVVAELATASLERGCPVLVFVPTRHRAPEVAEYLSQTVPDARTKWVHSGDAMRDS
                     KRRMLAEGECEVLVSTSVMERGITVDGVDVIVADADWETVFDCRALVQMAGRVGRTAS
                     NPTGSVWFTCSRENRQIQAAREMIDEMNRDAASRGFLNAGSEV"
     gene            complement(156459..158627)
                     /locus_tag="PLU15_00615"
     CDS             complement(156459..158627)
                     /locus_tag="PLU15_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOO29455.1"
                     /translation="MDLVEGIIDRVTFRDEDTLYTVARISTDSGMMTAVGPFSSATPG
                     QAVKLTGDWVVHRSYGRQFRVAKMEVIAPATVEGIEKYLGSGLIPGIGPVTAKRIVEH
                     FAMETLEVIQSCPDALLKVPGIGPKRAAQIVKAVEEHKSIEKVMVFLQSHGVSPTYAA
                     KIYKRYGDDAVAIVSRNPYQLADDIYGIGFKTADKIAREVGVDKDSPYRVNSGIKYWL
                     AKASDDGHCYSVAEDVIREVAAELDVSEDLVKQGLGALEESGEVFCERESSVWLGPFY
                     HSERGVAKRLWELNHGRMWQLDALSDEELGRAQAEAGLTLGAQQKQAIVRAFQCGVMV
                     LTGGPGTGKTTTLKLMIDLFEGRGLRVMLAAPTGRAAKRLSEASGRQAKTIHRLLEFE
                     PGEEGWRFARGVSSPLECDVLIVDEMSMVDILLMNNMLKAVKPGTRLILVGDVDQLPS
                     VGPGNVLRDIIASGVVEVVVLDEVFRQAPGSMIVSNAHRINRGEFPHLRGARDFFFVD
                     KEDPEEVAQAVVDTVKSRLPRYLKCNPVDDIQVLSPMRRSVTGVDNLNVLLRDALNPA
                     RQGVSELRYGATVFRRGDKVMQIRNNYERRVYNGDMGRIVWIDPEEGEVVVRYPEAEG
                     DRNVTYLQDDLDELVLSYCVSVHKSQGSEYTAVVIPVTTQHYVMLQRNLIYTAVTRAK
                     EAVVMIGTKRALAIGIKNASLLKRRTMLAERLRDLAESTV"
     gene            complement(158714..159913)
                     /gene="metK"
                     /locus_tag="PLU15_00620"
     CDS             complement(158714..159913)
                     /gene="metK"
                     /locus_tag="PLU15_00620"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOO29456.1"
                     /translation="MSEKRLFTSESVTEGHPDKVCDQIADAVLDAILEKDPWARVACE
                     VCATTGLVLVMGEISCHCYVEIPEITRETIRDIGYTRAKFGFDGDTCAVITSIDQQSP
                     DISLGVDRALEVRSSCGGGTRGADECDLIGAGDQGMVFGYASRETDELMPLPITLSHN
                     LARRLAEIRKTGVVDYLRPDGKTQVTVEYENGRPVRIDTIIVSAQHDDEVSQETLTSG
                     IIEYVIKPVIPSDLIDADTRILVNPTGRFEKGGPSADSGLTGRKLIVDTYGGMARHGG
                     GALSGKDPTKVDRSAAYAARHVAKNLVAAGVADKCEVQIAYAIGVARPVSISVDTYGT
                     GAIPDERITRIVREVFDLRPEAIIRKFDLRRPIYRQVSVYGHFGRPDLDLPWEALDMV
                     EEVRKRM"
BASE COUNT        37581 a        48262 c        37668 g        36467 t
ORIGIN      
        1 cttcttcgtc gctaacccgt tccctctccc cgtcctactc taaagttaca ccatcctcct
       61 tcggccccta caggtcatgc cgtaatatac aatgcaaagc cccagggcct gccttgttga
      121 ccaaggcaaa ggccctggat tgtcattctc tacactcgat gcatcgcctt gttaaccgcc
      181 attactatag ggggtatcaa aagcgcagac acaatagcct cgggaacgcc ctgcataact
      241 gcgatggaaa tcgctccagt ctttcccgca agcggaatgt acccaaacgt aaccgccaat
      301 gcaagcgttc ctacggtgtt cgtgatgctt cctgcggcag cagccaaagg caagctccac
      361 ggtttcttct tgagggcaac gtaaacccag tacgatacgg gccctatcag cagtcttgcg
      421 gggataacta cccgccagtc gcccatgaac ctcagagtga acaacccgaa gataagccca
      481 accatcgatc ctatccccga gccctcgagg attcctgcga gaatcacggg aatatgcatg
      541 agagtagctg cgccagccgg tgtgggcaat ggcacgaaac caacgcctgt ggccccaaga
      601 actactgtca acgctgcaag cacacctgat acggcaatct gctcagtctt gaccttcaat
      661 cacatcgcac ctccgttcca gcccctctcg ggtgccggac gaaataagac ggcaaggcgc
      721 accctgcttt tccaaacctt gcgccacgct ctcggtcaat caagatccga ggctacttcc
      781 gccacatcgc aggctgccac atagccgtct acagaacgag acgccctcac tgctctgacg
      841 acggccgctg ccagaacctc cgctgccaaa gaccctatgg cagtgatatc tgcagctcga
      901 tcacgggata gcgatctctt cccagtggaa aggcaaaaga ctgtgtcccc atcatacatg
      961 gtatgtgccg gaacaatcgc ccttgcaaga ccgtcatgag ccatccctgc gaccttattt
     1021 gcgccctcct tatctagcat agcatctgtg gccacaactg caactgtcgt attcattcca
     1081 gctaggtctt ctggatcctt agccgtatac acgtccattg ggccacgtcc ggacctgcct
     1141 gccctcctgg cggctcccgg gccaatgaac ctcttctccg ccctgtcgta ggctcctgcc
     1201 accacattcc ctgtccatgg atccacgata tcacccaggg cattcactgc cacaatagcc
     1261 cctacatgca cgggcttgcg cctaccgggc tggggaagct cggctacaca agttccgatt
     1321 ccgcccttca tcgcatatcc atcgcccaac atcttgccga ctgtagcgcc agcgcccgct
     1381 cctacattac cttcggcaca tgctccagac gatgccgctc ggcatgccct atatcccatg
     1441 cttcgatcgg gacgcacttt ggggtttccg attccgaggt caaatattac agccgccccc
     1501 acaatgggaa cgactccgaa tggagtttcg aacccaacgc cctcctcctc cagaaactgc
     1561 attgcccctg aagcagcatc caggccgtaa gcgctcccgc ctgagagcac tacggcctgg
     1621 attctttcaa ctagattgca cggacgcatt aggtcagttt ctcgggtgcc cggggctgaa
     1681 ccccgcacat ccacacctgc aacggctcca ttcggagtta atacgactgt gcagcctgtt
     1741 aaggcatcat catcttgtgc gtgccccact agcacaccca ggacctctgt tatgccatgc
     1801 aagtcgcatc tcccccgtac tggtctccca gaacttcaga ttacctaata caatagaaag
     1861 cgtcgagtcc tgcgtaaacc gctgcgtctc ctagctggtc ctcgatcctc agaagctggt
     1921 tgtacttagc cactctatcg gtccggcaag gcgcgccagt cttgatctgg ccagcattgg
     1981 ttgcgacggc aatgtctgca atggtagtat cctcagtctc tccggatcga tgggatacca
     2041 cggcggtata gcctgcgcgc tttgccatct ctatcgcatc caaagtctcg gtaagcgaac
     2101 caatctggtt gaccttgata agcactgagt tcgcgacgtg gcgttcgatc gccttctcta
     2161 tccgctcaac attcgttacg agcagatcat caccgacgag ctggatcttg cctccaagcc
     2221 tctcagtggc gattttccag ccatcccaat cgtcttctga aaggccatct tcgatggata
     2281 tgatcgggta tttgtcagca agggcttgat aatagctcac tagttgctct gaattgtatt
     2341 ctacgccctc tccctcaagc cggtatttac catcgcggta aagctcgctt gccgccacat
     2401 ccaaagctat gtacacttgc tcgcccggac cgtagccggc cttctcgatt gcttcgacta
     2461 tgaccgcaat tgcctcttca ttggaagata gattcggtgc aaacccgccc tcatctccta
     2521 ctccggtaga aaggccacgt tttttcaaca ctgacctgag cgaatgatag atctctgctc
     2581 ccattctgag cgcttccgag aagctatcgg cgccgacggg gactatcatg aactcctgaa
     2641 tgtcgacatt gttgtcggcg tgctctcccc cgtttagtat gttcatcatt ggcacgggca
     2701 atgtccgggc gcccaccccg cccagatact gatagagcgg caagccaaag aactcggcag
     2761 cagcatgcgc cactgccaaa gatactccaa gtatggcgtt ggcgcccaga ttctccttgt
     2821 ttggcgtgcc atcaaggtct atcattgttg catcgataat cgcctgctca cgtgcgtcca
     2881 tgccgattat ctcatctgct atcgggccat tcacgttatc aacagccagg gtcactcccc
     2941 gccccatgta gcggtccctg tctccgtctc ttagctcgag agcctcatgt tgacctgtag
     3001 aagcacccga tgggacagac gcccgtccca cgacctcatc aaacaatgtt acttcaacct
     3061 cgaccgtggg gttaccccgg gagtcgagaa tctcgcgcgc atatacattg gtgattatag
     3121 acatcttcct agtgcgagat aatctcgcct gcgctccttt ccctttgtgt cattccttcg
     3181 agtcaatcat agccgcgatt attcccataa agtcggcagc cttcaaactg gcgcctccga
     3241 ccaaggctcc gtctatatcg ggctgccgcg cgaattccgc catgttccca ggcttcacgc
     3301 tgccgccgta ttggatcctg actgcctcgc tcagatcgcg cgaatacagc tcagctatgg
     3361 tgtcccgaat caccctggcc attgcgttag catcttcgcc agtagacgcc cgtccggttc
     3421 ctattgccca caccggttca taggctacca ctgctttact cgcatcttcg gcagataagc
     3481 ctgcaaatgc gcctcttacc tgtcgccgta ctacgtcatt ggctaaacca tcttcacgtt
     3541 gagcaagggt ctcgccgacg cacactatgg gcttgagtcc ggccgcaacg gccgccttca
     3601 tcttccggtt caccgtatcg tcggtttccc cgaacatggc acgccgctca gaatggccta
     3661 ttatgaccca acgacatcct gcatccagaa gcatctcaca ggagatttca cctgtgaagg
     3721 caccgcttgt ctcccaatga acgtcttggg ctccgacgtc tatccccgat tccgcaactc
     3781 gatccgcaac agcctttata gcggtaaatg gcggacacag aacgacatcg cacttctcta
     3841 tgaacctcgc cgcatcgtca gcgagagcgc cgcccagcgc cactccgtcg ggtatcgtct
     3901 tattcatctt ccaatttcca gcgattatgc ttcgtctcac ttcaaaccct ccatgtcgcc
     3961 ttcatccctg agagcagcaa cccccggcag ttctcgtcct tcaaggaatt ccaaagacgc
     4021 ccccccgcca gtcgacacat gggtcatctt gtccgccagc ccgtactgct cacatgctgc
     4081 cgcagaatcg cctccgccga caatagtcac tgcatccgac gaagcaagag cctcggcaat
     4141 tccccgtgtt ccgcgggaaa atgcaggcat ttcaaagacg cccatcggtc cattccagat
     4201 gactgttccc gctgagcgga tgaggttgga aaagcgctcc actgtcctcg ggcctatgtc
     4261 aagccccatc cagtcagcag gtatcccatc tgcgtccaca atcttgtagg gagaatctgc
     4321 atcgaagcgt tcggctgcaa cgatgtcgac gggcagttcc agcgtgaccc ctcgatcctt
     4381 cgccttggcc atcgcacctc tcgcataatc cagcctctca gcatctaata tggactttcc
     4441 gatctcatag cccttggccg caaggaacgt gtaagccatc cctccgccga ctatcaagcc
     4501 gtctactttg tctaggaggt tgtcgatcac acccagctta tcagcgacct tggcgcctcc
     4561 aagcactgca atgaaaggcc gtaccgggtc ttcgattgcc ttgcccatta tttcgatctc
     4621 ttttttcatc aggaagccgg caacgcccgg gatataatgg gctataccct cggtagatgc
     4681 atgcgcccgg tgcgccgttc caaaggcgtc attcacatac acgtctgcaa gcgatgcaag
     4741 cgcctccgca aatctaggat cgttagcctc ttcttccctg tgaaacctca gattctccag
     4801 aaggacaaca tccccaggct ccatttcgcc cacagcttga accacttccg ggccaatgca
     4861 gtcacccagt ttcctcactg gctttccaag gagctcggaa agcctttccg cgatcgggtc
     4921 catagtggca gacggatcat atttcccctt aggcctgccc aggtgagaag cgagaatcac
     4981 cttagcacca tgatccaaga gatactctat cgttggcaag gcggcggcga ttcgtctgtc
     5041 gtccgttata ttacgctggg cgtccatggg aacgttgaaa tcaaccctga cgaaaacacg
     5101 ctttcccgcc acatttatat cttcaacagt gagcttgcgc aatgcacatc agccccttca
     5161 cacgcaacga tttacatctg cttggcgatg aatgacgcca gatccaccac gcggttggag
     5221 taaccccact cattgtcgta ccacgcaatc accttaacca tgtcatcctc cattaccatc
     5281 gtgcatagcc catccacgat cgaagaatga ggattgtgat tgaagtcgtg ggacacaagc
     5341 tcctcctcgg tgtagtcgag gattccagca agcggacctt cggctgcagc tcgaagcgca
     5401 tcgttcacat catccaccgt tgccttcttc tccagatcca ccacgaggtc aacgacggag
     5461 acatttggag taggcactct gagcgcaaat ccgttgagct tgcccttgag ttcaggcaag
     5521 actagagcca cagcagcagc tgcgccggta gttgtgggta tcatcgacat tccagcagcc
     5581 cttgccctgc gaagatcttt gtgggcaagg tcgaggatcc tctggtcatt cgtatatgag
     5641 tggaccgttg tcatcagccc gcgcttgatt ccgaacgtgt ccatcaggac tttagcaaca
     5701 ggtgcaagac aattcgtagt gcatgaggca ttggagatga tgtgatgatt ggcatggtca
     5761 tacttgtcct cattgacccc catcactatc gtgatgtctt cgttctttgc cggagcactg
     5821 atgatcacct tcttagcgcc gccagccaaa tgagctgatg ccttatcctt ggaacggaag
     5881 accccggtcg actcgactac tatgtcgact cccagatcgc cccaaggcag ctttgccgga
     5941 tccttctcgg caaatacctt tatcttcttt tcgccaatct ctatgcagtc gtcatctgca
     6001 acgaccttgc ccggatatgt tccgtgaact gaatcatact tgaaaagatg tgcattggtt
     6061 cgggcgtcca tgagatcgtt tacagccacc acttcaatgt catcatgtcc gtctgcagcc
     6121 ctcaaaacca acctgccgat tcttccaaaa ccattgattc caactttaac tgccattgtt
     6181 ccccgccccg atgggcggaa ctcacctcca ccttcttagt cgattcttcg tttgatccgc
     6241 ggacaagatt cctcacgacg tccttgtcgc gagctcgagg ctacgcgtcg tcaacagttt
     6301 tccccagcgc ctcacgcgca gccccctcgt ccgtcactac tatatgggaa aacccattgc
     6361 gcaatactgc cgccagcgcc tgacctttgc tggaccctcc gcccacacac attacagtct
     6421 caacagctga cagatcagac acacgcaaac ccagacttga tgtaatgtac actacctcgc
     6481 cgtctcggtc aaagtaaaat ccaaaagcct cccctaccgc ccccaagctt gctatctcct
     6541 cgatctccga aggtgacatg ttccgcctcc gcgccatctc cactgctgtt cctacaccat
     6601 gcaccactat cctggattgc ctgattgtgt cgagcacctc tttagtgcga ggttcctccg
     6661 ccacactggc cgccaattca tggccaagat cgtccgggag attgaggatt ctgtagacgc
     6721 cgcccagttt tttcgccatc agagcagcga ctgtatcagc ctgcttttcc acgtcctcgc
     6781 caaggcttcc cctggccgga accacgacga cattgcgttt ccccgaagca ggaaccgtct
     6841 gagcggcaag ttccgccatt gtagttccgc cacacactgc aacaacatca ccgtcggata
     6901 tcatatcccg cagaacacac gccccgacac gggccatctc cttcttcact gcctcgtcct
     6961 catcggagtt gccggtgatg actatggcgc gttccagatc gaactcatgc tgaagaagat
     7021 cagcaagccg ctgagcgcct acgagaagtc caacgtattc agccagatcc ctgaggatat
     7081 cttccccatc ctctgacagc cggatcccat cagggcctac aacgagtaag cctgcagtgc
     7141 gcagcacatt ctcttctcgc cttacatggc gttcgctttc gcccagcgac tgcgctagag
     7201 cgcgtctccc aatgggctga gcatgggaaa catggcggag aatattgtat ctcagaatga
     7261 tgcgatccga gaattccggc atcactctgc gctgtaacga aacgatctct gcagcgctca
     7321 gcattcgcat ctcgctggac attttttgtc ccgcatagtc aaattgcgtc ccacccatgt
     7381 caaataaaat tctgtgtctc cctcaaaaat ccttctgccc catggaaaaa aggcctcagt
     7441 atcttcgacg cttcgtggaa ggcggcaaca tcatctcatc cctatacttc gccacagttc
     7501 tcctggatat cattatgccc tcgcagccca gcgcatcggc tatctgttgg tcagaaaaag
     7561 gggctttcgg atcctcagag gcgatcatct cgcctatgcg ttttttcaca ctggcactgg
     7621 ccgcggcatc tccgtccgaa gtgctcaccc cggaatcgaa aaagaacctt agttcaaata
     7681 caccgcgagg agtctgcgca tactttcccg ccgtagctcg gctgacagtg gattcgtgca
     7741 ttcctatgtc gtcggcaacc tctcgaagag tcagcgggcg catgtggaga atgccacggt
     7801 caaggaagtc acgctgatgt ctgactattg attctgatac cttcactata gtggaacgcc
     7861 gctgttccac agaccttaca agccaaagcg cggaatcgag cttctccttg atgaaatcct
     7921 tcgctgcact gtcaacgttg gcgtctctca acatggatct gtatttctcg tttatcatca
     7981 gtcgcggaat ggcagagtca tttggaatca caatgtactc gccctcaacg tattcgatga
     8041 aaacatcagg cactatgtat ctcgtctcgc cgcttcctgt aaacccagca cccggacgag
     8101 gattgagagt gcggataaaa tcgacggccg cctgcacttg ccgaacgtcc acacccagct
     8161 ccgaagagac cttaaccatg cggcctgcag caatatcctc aagatggcat gagactatct
     8221 ttcgcgcaag gcgacacagg ttatcttcga ctcccgaggc gcagatctgc agatccagac
     8281 attctcggat gctacgcgaa cccactccaa caggctcaaa gctctgaata agggataagg
     8341 cgcgctccac gtcgtcaatt tccactccaa gggacgccgc gaccctatga acatcacctc
     8401 ggaaaaaccc atcaccgtct atgcacccca ataaatactc gccaatccgt agagtacgca
     8461 tgtcgctgca ggccagcctc agctccatat ggtactgctc ttcaagcgat ggttcccatt
     8521 taaccagggg aatcggatca aaatcgacat tagggtcaat cggcccacga gtccaaccca
     8581 cgtccgtacc gtcctcaaag aagcgctccc agtctatctc gttctcatca tcttcaggat
     8641 tagcgtcgct ttcatggaca gcattctctg gaccagcttg cacctgctta tcgacgacca
     8701 ttccaacgtg accttcttcc tccagaagcg ggttctcaag aagttcgttg gaaacatact
     8761 ccgcaagatc gaccgtggaa agttgaagca ggtgaatagc ctgtctcaat tccggggtca
     8821 tgacaagctt ttggacctgt tgctgcacga gcacaaaaga catttccacg gcgattcacc
     8881 tcaactttcc tgctgcccgc ggagcctgtt agcaatctca gaaatcctcc tcattcgatg
     8941 gttcacgcct gacttgctca gtttcggcga aagcatcgtc ccaagttccg tgaggcttgc
     9001 ctccggatat tccagcctaa gccaagcgat ctcgcggaga gccggaggaa gccgccctat
     9061 acccatcatc tcgtctatga gctgaatatc ctgaatctgc ctgactgatg cctccaccac
     9121 tttggaaaca tttgcgtttt cggcatttac tagcctgttc acatcatttc tgatcgatcg
     9181 gacaatcctg gtattctcca attccagcaa cgcattggct gcctcgatca cgcgaaggaa
     9241 ttccactaca tcgtctccat gcttaatata aaccacgctg cgctcccgac gttgaacaat
     9301 tccagcccga atatgaaatt ggcgcaacaa ctcgacaatg tcgtcagcat gggtccgata
     9361 tttcaaagcc atctccacgt gatatccttt ctccggctcg cccatataac cggctcctag
     9421 aaaagttcca cgcaaataag ccctcataca gcaacgattt gcgacaatag cggaatctat
     9481 cccgctggcc aagccgaact ccccatagat gccgaggagc tcgagaagtt ccatggcttg
     9541 atcgtgatcg ctgatgagga ctatatatgt attgccccta ggtagaccac tcccctggcg
     9601 cactgcaatc tgagtgtcag gacgtcccac gtcacgcaga agcataacta cttttcgggc
     9661 gacaccggca ttggattccc gaatcagcac cgaaatccgc ccctgagata tctgtatgac
     9721 tccgtcagtc ttgaccaaag cagagagctc agcaacctgg caacatctct gtgtcggcat
     9781 aacccttatc agttcgtcct tcatgcgcga atagaagtcc acgcaaacat cacctctgcc
     9841 aatagcgcgc cctgctattc ttcctcagct ggcaaccgat tacctccttc ccgcgacgct
     9901 tggatctgcg ccaaatgacg tcatacttga acactgaagg agaatacgat ttaagcgatg
     9961 cgacttgacc agcaaaaaca ttggtgattc tttttttggg gggatgatcg aatgagcatt
    10021 atgtggctgc tgatgttggc ttcaggcgcc attgtagcca ttatcgtaaa aggccctgcc
    10081 agcgttacga gtatcgcatt gtcatcgaca agagaagcag ttcagttatg tatagggctt
    10141 gccgggtcga tcgctctttg gtcggggatc gcgaaaatag ccgaagaatc ggggctcatg
    10201 tcacgactgg cagatctcat gcagccgatc atagccccgc ttttccctgg gatatcccca
    10261 cgttcccacg ccatcggtta catggccact agcatggccg cgaatcttct ggggcttgga
    10321 agcgcagcga cgccgttcgg gctgcgtgcc atggaagagc tggcagctga agacaagcgc
    10381 cactctggaa aagcctcgct acgcctcccc gcgcaacatc aagaagccac cgacgctatg
    10441 tgcacatttg tgataatcac cgcatctagc ctcacattag ttccaaccac agtgctttct
    10501 ctcagagccg aactgggctc cgcagatcca ggctccataa taggagcaac aataatcgca
    10561 actagttgct cgactggcgc agcaataatc ctcgaccgcc tgctttcaag gcggaggaga
    10621 cggagatgat gcaactgtcg tcactaccct atctcctgat tcccctgttc cttttgggaa
    10681 tccccgcata tggagcgtca aaaggcatcg atgtctacgc ttgcttcata gaaggcgctt
    10741 ccgacggcct gaaagtggca gcaaagatcg cgcccgccat gatcgcgatg ttcttggttg
    10801 tggggcttct acgagactcc ggagcattgg acatactggc gcaggctgtt ggccccgcaa
    10861 ccatgaagct tgggatcaac cccgacctcg tgatgctggc cattctcagg ccaatctcgg
    10921 gatctggcgc gctcagtctc ctcgccaaca tcctgaagga tctgggcccc gataccttcg
    10981 atggcaaact cgcctcagtt atacaaggag cctccgatac ctctctctat atccttgcgc
    11041 tctatttcag ctcagtcaat gtatcaaagt ggcgccattc agcagtggca gcgctcctgg
    11101 ccaacgcggt cgggttcgtg gccgctatca tcgcctgtaa tatcatcttc cggtgaacac
    11161 gtgccttacc gccaatgcca ttatctcctt agagagcgct tccgggtcat gtctgacaac
    11221 gttggtctgg ttgatcaccg gagcctccac cacactgacg cccatgctct ctagggcctt
    11281 gcaatcccat gcaactggta tagaaccctc tcggcagtaa ttttcaagaa cccgatcaga
    11341 tacgggagag ttgttcacaa tgacccaatc aactatatca gcgccggcgt gcgcgtatat
    11401 cgcggccaag tgatcctggg ctgtataccc ctgggtctcc ccgggctggg tcataacatt
    11461 gcatacatag accttgactg cattagacga agctatggca tcagcaacgc ccgaaacgag
    11521 aaggttcggc atcacgctcg tatacagact tccaggccca agcactataa cgtctgccgc
    11581 ctctatcgcc tccaagattt cgggaagcgg agagcatgta gccggttcca gaaaaacgct
    11641 tcggatcgcc cgcctactgc tgcttattga cgtctcaccc atcacaacat ttccatcctc
    11701 aagattcgct ccgagaacgc aattatcgag ggtgcacggc aacacgcagc ctctcaccac
    11761 cagtaccttg ctcgattctt ttatggcaag ttcaaagtcg cccgttatgt cagacatagc
    11821 tgcgatgaac agattaccaa aattgtgccc tgccagaggc ccttgaccgt taaacctgta
    11881 atcgaaaagc tctttcataa gcggttcggc atctgcgagc gcaaccaggc agtctcggat
    11941 atcccctgga ggaagcatgc ccatttcctg acgcaacctg ccagaacttc caccgtcatc
    12001 tgtgactgca acaaccgctg tgattttatt cgtatattcc ttcaaacctc tcagcaaagt
    12061 agaaagcccg gtcccaccgc ccaacgccac aacgcgcaat ctgcgggaca agggtttagg
    12121 cacagggctg tgacgcatta gagtcccccc tacttcgatg cgggttgatc cccgctgccg
    12181 atatcgatat ccctatgcat tgtatgcgtg cggtatccga gttcgcgcag aggccgcgac
    12241 aactgattcg cgataacaac agatcgatgc cgtcctcccg tgcatccaat cccaacgacg
    12301 agattgaact tgccctccct ttcgtactcg ggcaacaaga atctaaataa atcaactagt
    12361 ttatcgagga atctcttcgt caccggccat cgcattacgt attcattcac ttcttcatcg
    12421 tttcccgtca ggttcctgag cgaatccacg tagaacgggt tgggaagaaa tctgacatca
    12481 aacacaagat cggcatcggc gggcacacca tatttaaacc caaatgagac tacagtcagc
    12541 tgtatagtcg gcgcaccgga attatctccg aatctccgta cgatctccgc cctcagttgc
    12601 ctagccgtca aatccgacgt gtcgatcacc agtgcagccc cagcgcgcag ccgagctagc
    12661 aactgtcttt ccagcttgag cccgtccagg attccgcctt ctggcgccaa tggatgcggc
    12721 cttctactag ccttgaaacg attgacgagg gtttcatcgg aagcttccag gaacagcact
    12781 ttgtagttga accccgaatc atccatagta cggaaaactg aagaaagctg gccgaagacg
    12841 tcctgtccgc gcacatctat aacaagcgcc actttatcaa tgcgacccga tgtttcggaa
    12901 agcaactccg caagtttggg aataagcatg accggaaggt tatcgatgca gaaatagcca
    12961 agatcttcaa aagccctagc cgcttctgat ttaccggctc ccgaaagccc agttattacg
    13021 atgcaagaaa gctcttgccc actgattgta gtttctgcca tgctctcatc ccctgatgat
    13081 catcgattgc tgttgtcaca ctgcaaatct cggatactca aacctcgtaa ggaaacaatc
    13141 acatctccgt cacactcgtt aatttcagcg acaactccaa tgtgattgaa agttcgaaac
    13201 aagctgatct ctaacagtga tccagatctg cagaagacag ggccagacgg agagatcagc
    13261 ccgggcataa aaaccccgtc gcatactcag tttgtacttc cgagtatatc acaggcttcc
    13321 ccagtcgatc catgtaaaga ccgaatcgcg ggaagacgat gcttccaaaa cctccagcga
    13381 tcgcagatcg gtttccctag ccatcgctgc ctgcgccaga acctcaaaat cggacagatg
    13441 ctgcgcgaac ctatcacgag catatcctcc agcttgaccc gttgtcacga ggaagggcca
    13501 atcgctcgct tccaacaaca tcagctctcg aagcgcctga tcggccatga ttttatggac
    13561 cccgtctagg cccccaatta ccccttcaag ctcgcggccg atagcagtgg ctctctcaat
    13621 ctcctgccac atccattccg tttcagagtt gaaccataca ccatgtcttc cgccatgccc
    13681 ccacgaacac tcagggagct ccagttcatc cacgggcggg tgcagcttca agtaagatcc
    13741 cagggtgcca cactcgacgc gattcgatgc acagagcgcg tccattgcca atgaaagcca
    13801 atcaacgcct tcatgccacc aatgcccgaa aagttccatg tcgtaggggg caacaataat
    13861 cccatgcctg cctgtcctct ctgcgtaacc accaagcctt tcctctacta caccagcgaa
    13921 gtgctgtgca tgggttcttg cgcttgctag agcagcctca gggtcgtaaa gctgcttctg
    13981 cccgaaatcc aggagtttgt ttgtaaccct ccagtaacga agtccggatg agtcgtcccg
    14041 cttgtggaat tcgaggtaat tgccgtcacc cggatacccc acatccctcg accatacctg
    14101 agcagctgtc acctcatcgc gcccgaaaac agccatcttg gagccacgca ggcgatacgg
    14161 catgagcgtt gccatactcg gcaagccagc cccaatgttg cgccgcaaag cctcaggaac
    14221 gccaccatac aggtttcgcg ggccaccgcc agctattgct ggagtatcga ctatgaaata
    14281 ctcgattcca cattcctcca gcgcgcgctc gattgcgcca ggcttgtatc cgcattcggg
    14341 aagccatgcg cccttgggca aaacgccaaa gtgccgttgg catgcctcgt aaccgacgcg
    14401 aatctgagtg cgaatacgtg attcttgagg cagcaatggc agataggcgt ggctggccga
    14461 tgtagccaag acctcaactc tacccgaggc ggcaagcttg gcgatggcgc ccaacaagtc
    14521 gccgtcgtag taatctatga atcgtttctt agcgttttca tagtattcga cgtacttcct
    14581 tgacagcctt gcaagcctgt cgtcgtggac taactcgtgt cgcatgagat cagcgcgggc
    14641 cgcattggcg cgagcgtcca tgtattctac gatgcgggac ttcatgtatg catccgcaag
    14701 ttgctctgcc aataccgggg aaaaactgag cgtaacattg ggtccgaccc ctttttcatg
    14761 gcaattcagg agccaatcca gtatagggat ataagtctcc accactgctt caaaaaccca
    14821 ctcctctcca aatggccaca ctccggatct gcggcaatat gggatgtgag aatgaagcac
    14881 cagagaaaac gttccaatgt ggtccatagt aaatacctcc ttgaagcata aactattgtt
    14941 gcccaaagcg agaagagtat ccggtatggg ataacccgca tttctcgcgg gcagactaag
    15001 cgagagattg acagacggcc aacaccagcc gttgagcctt atgtgttttg gcgcttaaag
    15061 ggaggttcca caagtgcagg actcgttcga taaagttagt ccgatagaca aaaaaggcgc
    15121 agggacgccc agcgtgtccc agcacgagca gcagatggcg gaagatgcca gaatcgaact
    15181 gagtccgccc gaagtgagcg atcagccgct tccacacaga cgcttcccgg agatcacgat
    15241 ccttgagacc gaaaacttac agatgaaccc cgctcggcgc cacaaaccca cggtcttgcc
    15301 gggtttctac ggggctacca gaatagtgct tcaagttcgc gatcccttct ggatgcacgc
    15361 atattgggaa atcgccgaag acatggccaa aaacctacgc cgccagttgg gagaagagtt
    15421 ggaccgctca cagttgacgc tccgcgtcca cgatgtaaca ggcgtcacat tcgatggcca
    15481 taattccgtc cgcagtttcg acaaagaggt ccatcctttc gcgaacaatt ggtacataaa
    15541 cgtggaggaa tcagacaggt cctactgtgt tgacctgggg ctggttacgg gcgcctcaca
    15601 attctacctg atctcgcggt cgaataccgt ccacacacca aggataggtc catcctggat
    15661 tgtcgacgaa gagtggctaa ccattcgcga gattgaggaa ggttacatat cgcatggcct
    15721 atcgccttca tcgccgggta tttccaggca tgcccgcgcc gaagaactgc ttcacgaaat
    15781 tgccctcgga tcgggaggag tgggagcagt cggaagccct atgggccaac ctggcgaagt
    15841 acaccgagcc ccacgcggct tctggttgag agccgatgcc gaactgatca ttcacggagc
    15901 aacggaagca ggggctaaag tggaagtatg cggcatgccg atgcctgtca gacctgatgg
    15961 cagtttctca gtccgcctgt ctctgccatt tggagaccac ccggttgaca tttccgccac
    16021 atccgatgac ggcatcatgc atagaagcgt ttcgtggatt gtctccagat cgaaccgggg
    16081 aaatgggagg tcgtagttgt tgtcaactcc aaatggctac cttgctcttg tcttgcatgc
    16141 gcatcttccc ttcgtgcgac acgccgacgg cgaacattac ctggaagaaa aatggttcta
    16201 cgaagcatta attgaaacct atatccctct ttacatagtc ctctcgcgac tggccgatga
    16261 agaggttcct taccgaataa caatatcggt ttcgcccaca ttgctttcga tgttccgcga
    16321 tccgctgctc cagcagagat tctccgacta catgaataac cttgtagagc tggcagaacg
    16381 agaggttgcg cgcacgcgtt ggttgcctgc attccatgat actgcacaaa tgtaccgaga
    16441 ccgttttcgc gaagcgcgag acgtttattg gaatcgactg ggcggcgaca tggcgaaagg
    16501 actccaagct ctttcagatt caggtcatgt cgaactcatc actactgcag ccacacatgg
    16561 atatctcccg ctgatggaag tttgtcccga agctgcccgc gcgcagattc ttgcgggaat
    16621 cgactactac aggcaagtat ttggaatcgc tcccaaaggt ttctggctcc cggagtgcgg
    16681 ttactcacca gggatagatg aatacctcag agaagccgga gtgaggtatt tcttcatcga
    16741 ctcccacggc atactcaatg cttcgcctag gcccagatat ggagtgtatg cccccgtgta
    16801 ctgccattca aaagtggcag cattcggtcg cgacatggaa tcatccaaac aggtttggag
    16861 cgcacaggaa ggttaccccg gggattacga ctaccgggag ttctatcgag atatcggata
    16921 tgagcttgat tgggaatcga tcaaacccct catggggaac tgccctattc gtctccaaac
    16981 tgggttgaag tactaccggg taacaggaaa tacagatcga aaagagccat atgtgcgtca
    17041 atgggcaatt gaaaaagccg ctacacacgc gggcaatttc gtcttcaaca gagagcgtca
    17101 agtagaatac ttagcatcag tgatggaccg caagcccata gtagtggctc cttacgatgc
    17161 agagcttttt ggtcattggt ggttcgaagg tcctgagttc ctcgattacg tgatccgcaa
    17221 aatcgcatac gaccaaaaca ccatagccct gttaacccca tccgactacc tggagctcta
    17281 tccgaaaaac caggtcgcta ctccatccat gtcgtcttgg ggatacatgg ggtacagcga
    17341 ggtatggctg gcagattgca acaactggat atacccgcac ctacacacaa tggcagggag
    17401 aatgacggaa ctggcccgct catacccaca tccccaaccg gagttgacgc ggcgcgccct
    17461 agctcaagcc gcacgcgaac tgctgttggc tcagagctcc gactgggctt tcatcatgaa
    17521 agccggaacc tgcatcgatt atgcaaaaaa gcggtttgaa acgcataccg cacactttct
    17581 ggggctgtat gatgacataa aatcatcgaa catagatccg gaatggcttt ctgagctgga
    17641 agaacagagt aacctgtttc ccaacataga ctacatggtg tacgcaggca gagcaactat
    17701 gtaagcccag gtgctcgaca gagggaagcc gcgggcgctc gccgcgcgcc atactttcat
    17761 gaagcaacac tcacatttga acttttcaag ccccatcgcc ctcaagcttg tgggcctgct
    17821 tcaatatcaa tgtcgggtta atcaggctgc tacgcataat tgcgcgttgc gcgctcttta
    17881 gaccgcaccc gcatctgata tggtttcagc aatcctactg acatagatcc cggctatttc
    17941 ctccgcaatc tcctggctat gcgcctcact gtacacgtgg aacaaaggct cttcggagtc
    18001 tggaagaatc aaagtccagc catggtcact gtagaccttt attccgtcga tcagctgaac
    18061 ctcctcacca tcagtctcct ctatcaacct cctcatcacc ctgcctttgt cctcccaggg
    18121 acaatccaca acttgtttgg tcatatatga tctcggcgca atttgcgcaa acgccgaaag
    18181 agtagtcttc tccctcgcaa gatactcaag gatcaccgca agagcaaaaa ccccatccat
    18241 tacaggctga aagtgaggca aactggcctc tcccattctt actcgcgcct cgatggcttt
    18301 ttccatcatg gctctggggt tcgcttgggt tcggactaca tatcctctga acgcagcagc
    18361 cagccgctct atcgctgagg acgctgtaac cggcacagcc atggtagtct cacgacccga
    18421 tgtaaggagg gccacacata tgagcgccag catctcttcg tttgagatat tcgaacccgt
    18481 ttcgtcgaca agcgcaagcc tttctgcatt tcggtcaagc agaaaaccga ggtccgcctc
    18541 tgtcacggcc actgcatccg ccacctctga cggtgcccag gcggtattgg agatctccac
    18601 atggtgcaga tcacatccgc aagcattgca gaaggcgtcg ataagtgctc ccacgtactc
    18661 cgagtcatag ccccctacca ccttcaggcc tcgcctccga accgtgtcag catctatcga
    18721 cgagctgatc gcatcgagat atctacctac catcccctcg gtggtgatag tgctgcccac
    18781 cttgtcagaa ttcgcgcgtt taaagtcctc tgccagaaag gcattttcta ctttcctttc
    18841 agttgccttg tctatattga tcccgctagc atccaggaat tctatgagac tcgcgttccc
    18901 gccagttgga gacacgcgca catgcactcc cccactagcc ccaatagcag gaaccgcata
    18961 tctggtaaca gcggttgagc ctgagcctaa gtcggccact gagatgcctg aggaaaggat
    19021 ccctgacgcc aatgcccgct ttatcatgtc ggacgcggga tgagcatcgc aggacaccgc
    19081 cactaccccg ctggataaac acgagccata ggcagcgcct agccgggccg caaagtccgg
    19141 agtgatctcg atgttagcaa gtccggtcac accaagacgc ccaaacaggc gcttcgacca
    19201 ggaggctccc cacactaggc tagcgctgat ctgtgcgccg ccatctaatg tcttgtcagg
    19261 ccatatcttc acgccggggc gcacttgtgc tcgttcaccc atggagcagc cggaacctat
    19321 aaccgctcct tcctgtatta gtacacctgc tttggcgctc accctacttc caaggattgc
    19381 gccatgcacc tccgcatttt gtccgatgaa acaacctgtc cacagtatcg atcttcggac
    19441 agatgcccct tctgttatga tacaagacgg cccaacaacc gagtattccc cgactgccgc
    19501 gccagcttcg atctttgctc ccgctcctat cactacaggc ggacgcaagg cagcgatgtt
    19561 ccctatctca gcgccctcgc taacccagat tccctcatcg acgaggtctc cggggatctt
    19621 cactcggacc tttccttcca agatatcctg ggacgtcttc cgatactgat ccaagttgcc
    19681 cacatcgcac cagtatccat cagctatgta gccatacatc ggatatcctt cagcaagtag
    19741 aagcggaaaa agattcttgc tgaaatcaaa ctcgcaggat gggtccaccc tctgcataat
    19801 ttcaggttcc agaacataaa tgccggtgtt tacagtgtcg ctgaacacct cgccccagct
    19861 cggtttctca agaaacctgg tgattcgcgc atcgccgtcc actattacga ctccatactc
    19921 caatggagtt gcaacccgtg tcaaaacgat ggtaactgcc gcccccctct ctcggtgaaa
    19981 ggtaaccgcg gaagtgagat cgatgtcagt gagagcatcg ccacttatga ccaaaaaagt
    20041 atcgtcgaga aaatccccaa gagctctcac gcttccagca gtgcctagag gggtattctc
    20101 tactgaatag gtcattgtgc accccacaga cgacccatcc ccaaaatgtt cctgtattgc
    20161 ctctggcatg taacagaggg tacatgccag atctcggaaa tcatgggcga caagtagatc
    20221 cacgacgtgc tccatcatgg gcctatttac aaccggaacc atcggcttgg gcatgctgca
    20281 cgtcaaaggc ctcaacctag ttccttttcc tcctgccatt atcacagctt tcacctgacc
    20341 atggcctcct ccgcatgtct ggcctctctg agcacttctt catacaccct gagggtacga
    20401 tcagctatgg cgcgccagtc atagatagtc tgcacgtctc tataagccgt ctctgccatt
    20461 tgcctggcaa gctcgggact gtaaagcacg tgcaaaatcg catcggcaag cgagttcgca
    20521 ttgccggcgt aacatttcca tccgttttcg ccgtgcctga ctatttcaga aaggccaccg
    20581 atatcagacg agacaagagg tgtttttgct gccattgcct ccaaagcagt tatcccgaac
    20641 ggctcataca agctcggcac gacagcaacc ctggcaagcc tgtacaacgc attccttgcc
    20701 tcatctgaga caaaacccac cattttaacc ttgccgccca accccatgtt ggccgcaagc
    20761 tcgcgaagat cgccctcaca tggaccctga cctgcaatga ccaacacagc agtaggctgg
    20821 tagctcagga tcttcggcat ggcctcgatc aatacctgaa cgcccttctc attcacaagc
    20881 cgcccgacaa agagaatcac gctgtcttct tccgaaagcc catacatccg ccgcgcaata
    20941 tcggcatcag catcctgaaa ctgctcggaa taaacaccgt taggaatgat tcgcagttta
    21001 tcggcgggaa tgccgaatat gcccgcaagt tcagcctgca tatggcgtga acatgcaatg
    21061 actcgccacg cttcataaca gaggaaccat tctatgtcgg atatccgtcg ttgagtggcg
    21121 ttatgaaggc cgccattcct tccccactcg gtagcatgaa tcgtggctac gagaggcgtg
    21181 gcataggctc ttttcatgcc gacaccggca aacgacgtca tccagtcgtg tgcgtgcact
    21241 atatcgaacg cgccgccctc ggaaacaagg cacgcagccc gggcaagcat tccataggac
    21301 aattgcattg cccatgttac aaaatcatct gtgcagggac ctacgggatc aactctgtgt
    21361 acccacactc cgcgatcgcg gtccatggat tgcgcccctt ccgcactgca cgtgattacg
    21421 tgaacctcgg cgcaacgcgc catagcgcga gcaagctcac atacatggcg ggccagtccc
    21481 ccaataacac gaggcggata ctcccagcta aacatcagta tacgcataaa acacccctct
    21541 ttgaaggaca tagatcgagt aaggtggcgc caggtaggca acccttggtc gctttgcttc
    21601 attgaagaga tgcaagatgc cgacctcgta ttttgtgagg catctctagc tcgaagggaa
    21661 tctaccccgc caggctttgg tgctctactg aacaggcccg gctcccccgt tcctatcagc
    21721 attaggatca ataactacca cggcctcatc tgccgctatg caccatgtct tcctgtcctt
    21781 gcatccctct ttgtttatga tttgtagcgt tccggatgat atagggctcc ccttgttaag
    21841 tctgtccgcc cgaaccctca ggttatcccg ctttcaggct tttccactca gcacagggtt
    21901 gcccacctga caggccaact tcggctcgca aaacatgttc ggcgaaccac aagactgcgg
    21961 tctgaacttc aaaccgcagt cttataattg gctatgaagg atgaattatg cgccggaaac
    22021 cttgcgtctt cgaaggtaga ctattacacc cgccaccact actccggcac ctacgccaat
    22081 ggcaacccac atcacaactg tcccgccgac tctggcataa ccaggcgtta tctccaccac
    22141 agaatcgcgc acttcgggaa agaactcgtg cttgcccgtg ccactgacat aagaacctgc
    22201 attctcgatc aaagcctttc tgtctgtaac caagactgca accagatcat atctgccttt
    22261 tggcaagggc gccccaactt ccaacgctga cttctcctgc cctttccttg ccttcttgag
    22321 cgtcgaggaa aaagccacca ttggcgtatc agtaacatat ccgtcgacaa actgacgaat
    22381 cgcgcatgcc ctcgtatagg tgggaagccc gacaactatc tcattgtcta cttcctgaat
    22441 ccaaaggacc gcagatggag aaggccatgc cttgctccgc tgaagttcaa agccgaaatt
    22501 cgcagaagca cctggcgcca tggcgaattt atactcaaga tcgagacgtc tgacaaagct
    22561 gtccaacgtg gaagagaccc gggatagggg cacaccatcg tcgcgatcac caccgacagt
    22621 gagtctcacg acagttccgg taaccacgtt catcaggcca aaactcttcc cctgcctaag
    22681 agaacttgcc actgctccat tgggatcata gtaaacgagg gaatccttac cgcctatcct
    22741 agtccaatgc gcatccgggc ataagccatt cgatacagca aagctgatgc gaccacgcga
    22801 tctgacggtg tagtcgactt cgagagcccc ttcttttgcc gccaccgatt tttcaattgc
    22861 ggtgccgcca tccttgaaaa caaacgtcgc gctgggagaa tcctcaccta tctcgacacc
    22921 aaattcggct gaagagagat caatcccgac cgcagcagga cctgaaatcg aatcaaccag
    22981 tgctctttga cggccgccgc cagccatgga ttcgttaaca aatatctccc caagatactg
    23041 ctgtaccgcg cttcctacaa gctcctcgcc gttctctaaa tcataccaat atagaagttt
    23101 tgcgcctatg ggcgagagaa cgaacatgtc tgtatcggtg atcatgataa cctcttctac
    23161 accatcgcgg ttgatgtcct cttgataagc tgaaactgtg gggtttacgc attgccacgc
    23221 ggcgagagct gacacgtatg cggccctgcc catctcccag ttggcatccc cggaaaccgc
    23281 ccctgcagaa ccagggcgac cgaacccgta ctgggatgat ataaagacca tctttgcatc
    23341 gtcgagcagc ctttgagccg aagacgtgtc tttcccggat gcttcagctt ttctgacagc
    23401 cgcttctaca gaatgaattc tggctctaat gtcagaatag agaacgcgca acgcctcaag
    23461 attacgagaa gaattgaacg ccgcccaatc cgaaaatccc tcatccgaca gagccttctg
    23521 aaatccccgg ggcaccccat ctgctatctg aatcacgctg cctggcggag gaccagcttc
    23581 cactctttct ctcaatgtag tcaccctgat ccattcctca tccgccaagg ctctgagaag
    23641 aagcgagagc ccttcccatt cagtcttgtc ctcagtcacc atgagctcct tggcgtacac
    23701 caccaccgca gagtcctgct tgtccccttc ataaagttgc tgaagatagt cgatgaccgg
    23761 ccgtgtggat ccggtgcgaa tcgcattgtc aactaaccga ccgaaatcag tatctgcctg
    23821 aaagagcact gcttcccttc cgccccatga gacgcgcatt ggagcatttg gaggcagcat
    23881 tccaagtcca ctggcttcga ctatcgtatc ctccaatatc gtatgggtgt atccgccgat
    23941 ggccacaggc acaatcacgt cctgtctcca cactccttga ggattccaaa acccggtggg
    24001 cttttcgccc aggaagctgc ttacagcctc agaccccaat gataccgcaa gctgagcatc
    24061 ccagggttcc atggatgcca agactccctc ggaatatgtg gcgccaagcg gctctatgac
    24121 tcctctgccg atcccatcac gcaacagctg gactgcgcga ctaccttccc atgccaaccc
    24181 tgtcagaagc gtcccggata ttccaaccga aaacctcata tcaggatatg aaaggaggac
    24241 gtcgagcaac ctttcacagg tcgcagatgc ttggccggcg ctgtcaggag atacgttccc
    24301 catgagtcca aaagcaaata caacatccaa gatccgctct gtgtcatcct cagagggatc
    24361 ctcctcactg agcatggcct cagcaggcac atgcggttcg ccaggttctg ccggctccgt
    24421 atcccagtcc cattcccaga tcggccagcc tagactggac tgcgcaggca caccaaacat
    24481 cagcacaaac aaggcgattg cgaatgcaca gatccttgaa gtagacttag cgtacataag
    24541 cgtgctcccc cttaacccct tccaacgttg ctcctgcccc taaagtagat gtatgccagc
    24601 gccgcagcgc cgcccacagc gataatgagg atgacggttc tgacggttag gtgcctcctg
    24661 tgcagctggg cattggccgg cacaatcctg gcgtatcgac gctcagacac atcccatgaa
    24721 cctagttgac tttcttggga atatacacca ctctccgggg cgagcatgtc tattacgttg
    24781 gggttgatgt tgagatcaga acctccacca aaaacccacg gacccgcttc ccccatgacc
    24841 ggcctgaagt tgtcgagccc atagccatcc tgagaagaaa cgagcacgta gtatttccag
    24901 gagtcaagag gttctccgat cacttgtcgc gacacagtgg cgcgtatagt atttccatcg
    24961 ggaagagcct caactgacaa tccttcgatg ataccagccg atgccggatt atcagatgcg
    25021 aaaaataccc ggctgccgcc ccaccccatt atcttgatat tcacatccca tgcatactcc
    25081 ggcgcgaaag agacgaaggc tccttccctt agagtatcgg ttcggccagc gccagaggtt
    25141 gtgtcgatat atatattgat gagctgatga ctgaagccct ctggcgcgtt ccaagtgttg
    25201 gaaatcttgg ccagtttggt gtcgaagtaa acattctcat catcgcagga aaccctgaac
    25261 cctattaggt cgaataatcc ggagaacggg gcgaatgccg ggttggtggg ataaacatat
    25321 gttcccggtc cataatcatc gccttctgga tcatcagctt cccagataag cctctgcccc
    25381 acccctgccg ggaaatgcgc cgcggacgtg cgcgctgacc acatcatcac cgccagcgcc
    25441 ggcaagagca caatgataag tgccaggcat agtcttttca cagtcttccc cccggataat
    25501 catatcctac cgctctgggc tcaatcttcc ccagactgct tctccatggc gtagcacgcc
    25561 gcgcccaaca tccccgcatc tacccctaaa gcagcaggaa cgatcttggc atctttagcc
    25621 atgaacggaa aagcgcgcaa atccactacc cgtctgaccg tgtcgaaaag catgccgcct
    25681 atctgggata cccccccgcc gataaccacc atctccacgt tgagcaaatt caccatattc
    25741 gcgaccacaa taccaagata ttcgaaagcg ttccgcaata ttgctgtcgc cataggatct
    25801 cctgcttcag cacaagagga tacgagtacc gcatcgatat tgtcgatctt gccaccggct
    25861 gccgaaagaa ttccgctgcc agcggtacgg gtagctgcct cccgcgcacg cttggcaatc
    25921 gctgtccccg aagccaagga ctcaagacaa cctctgttgc cgcacccgca cataggaccg
    25981 tcaggcacca cggtaacgtg acctagttcg cctgcaccgc ccgtggttcc cgaataaagt
    26041 ctgccatcga ggataagtcc gcttccaata ccagtgctca ccgttatgta gaccatgttc
    26101 ttcgtcccgc ggcccactcc catacggttc tcagccagtg cggccacatt ggcatcgttt
    26161 tcgagatata ccggaagtcc ggtcgcaaca tgtatcgttg atgtgactgg gacatctttc
    26221 catttgaggt ttggcgcatc cactaccata cccaattcct tatcaagagg gcctggcgac
    26281 ccaatgccta tggcttcaac atcatcgatt ccccctggaa gtttcgacgc aagctcgcta
    26341 attgaagcga taatcctctc cattacagca tcctgcccct cttcgggaca tgttggcatc
    26401 tgaacattgg ctagcggaag gttatcctca ttgaccagca ccgaagcgat ctttgtgccg
    26461 cccaaatcta caccgatgaa atggcgtttt ccatgctcaa tcttgttctt catttcagcg
    26521 ccccctgtcc aatatcagtc cgtacaatag catgccgggg ccaaagagag gccccggcat
    26581 tattatcccc atgtctactt aatggcgatg gcattctccg tgcttgcttc gaagagatgg
    26641 cactcttcca tattcaacac gactgcatgc ggttccccgt ctcgcgccgt tgtccgaggg
    26701 ttgacccttg ccacaaagga atgcgggccc accgtcaaat acaggtaggc ctctgcgccc
    26761 ataggctcaa ccacgtcaac catgcacttg accgtcctgt ctgcaggcgc atcttccacg
    26821 aaatccgcat cctggatatt ctcaggacga attccaagaa ccattctctc gtcaatgtac
    26881 ttgccgagat ccttgaacct gttccctggc accgttattt ggaagctctc gcaatccatg
    26941 atgtaaccat cacccttttt gcgcagaacg ccagggatga agttcatcgc cgggcttcca
    27001 ataaagcctg cgacgaacat gttgtctgga tactgatata tctccagcgg cgcaccgacc
    27061 tgcttgatga aaccatcctt cataactact atgcggtcgc ccatggtcat ggcctcagtc
    27121 tggtcgtgcg tcacatagat gatagtcgtt tgcagcctgt tatggagctt tgacaactct
    27181 gacctcatct ggaccctgag cttcgcatcg aggttggata gcggttcatc catcaaaaac
    27241 accttgggct cgcggacgat ggcgcggccc acagccacac gctgcctctg gcctcctgag
    27301 agcgccttcg gcttgcgatc gaggagattc tctatgccga gtatcctcgc ggcatctttg
    27361 actctctcgt caatctgctc ttttggaaac ttgcgaagct tcaggccgaa agccatgttg
    27421 ttgtagacgt ccatgtgagg gtagagcgcg tagttctgaa ataccattgc gatatcgcgg
    27481 tcttttggag ggatatcatt gaccagcgtg tctccgatgt atatgttgcc cgaggttatc
    27541 tcctcaagtc ccgccaccat tcggagggtg gtcgattttc cgcatccaga aggcccaacc
    27601 aacactaaga attccttgtc cttgatctcc aagtctgcat cgtttactgc gactacgttt
    27661 ccgaacttct tggtcacatg ctctaatgtg acaccagcca tggtgtaacc tccctttata
    27721 ttaatcggtg gcatatgagc ccacctgcta acttctgata ttcaacaagc gtcgcaggaa
    27781 tccttcaacc tatttgtgcc tgagcgcgta taatacctca gaaaggcaat aggcggtgat
    27841 ggaaattgga tgaacgcaag ccgctcgtgc ttatgcgcgg gatgatagcc atacccgtgg
    27901 gaaatctcca ggaacaactc gccttcaagg gtttcccctt gggcgaagtg gacaacgtgt
    27961 tcggaatacg aactgaactc gcagtaaagc aattccaggc tagtgtgggc ctgccagaaa
    28021 ccgggatcgt agacgcccag acgtggagac tcctctttga cggccttccc cttccggacg
    28081 aggtattctg tcccgatgac tcacttcgct atgacaatac tgtggattac cgtggatatt
    28141 ccgatcggtc ctccgcaggc agaatgtgta tttcagcaga taaagacaaa tccacaaacg
    28201 gcgggtctga gctcgccctt tccgctagct cgaagagtcg gcagggccca tcaatccatg
    28261 tcagcctatc ccaacgcaga ctcacactcc tgcagagcga aagggagtcg gcacagtatc
    28321 ctgtggccat aggcaaacca gcaaccccaa ctcccgtggg caactggagc atattgaaca
    28381 aagccctcaa ccccggcggg gtttttggca caagatggat gggcttcact cgacaggggc
    28441 acggcataca cggcacaaat caaccacagt ccatcgggca tgccgtttct aatggctgca
    28501 tcagaatgta tactccggac gtagaatccc tttttaccca ggtatcggtg ggtaccccgg
    28561 tcgtgattga cgcgggagtc ccttttccga cccctgcccc gggaacatat attgtaaggt
    28621 ccggcgacac gctctatctc atcgcccgta gattcggcac gacagtggag gcaatacggc
    28681 ggacaaacaa cttgactacc gacattatct cagtaggcca agttctgttc ttgcctggac
    28741 cgacaggagc tccacccata atcggcacag attacgttgt acagccaggc gacacgcttt
    28801 tcctgattgc ccggagattc ggcacaacag ttcaggcaat tatgtctgca aacaacctga
    28861 caagcacgaa catatatgtt ggacagagac ttcgcatacc cggaggtccg cggactgccg
    28921 cgcagatgag agctcagagc ggagcatccg aatatgtcgt tcagccagga gacacattgc
    28981 actcaatatc ggcagcattc ggcattccag tggaggcaat aatcgcagca aataccatga
    29041 tcagcagcga catatacccc ggccagctct tgcgtatgcc ataaaaggag caaacgagtg
    29101 atacggtgag ttcgcaatgg tatggggaag catagcgttt cttcggcaca acccgacgag
    29161 cactcccgcg gacaagcagg gcatccgctg atgggattga tcgtagatca gcagaacatc
    29221 tttgcagcac tcccaaagtg gatggaatga ctctggagaa actacaggag ttttatgctg
    29281 tttggcgtcc caacggtttt ggaccagcct attcactaga ttcttacatc cgccttcagc
    29341 ccggaggcct cagattcaag ctacggcttc cgcgccattg aacgggccaa aagcaaaggg
    29401 atacacatgg ggttgtaaat acggatcttg atttcgcaga gcgatggaca agagactgct
    29461 caagcttatc cggcgaggta ggagggcgga cagaccgccc ccctactcga ttcttcagtg
    29521 attccgccca gtccgcggat cacgaagctt gccggtgatc gatactagaa ctccacttcc
    29581 ataccggcgt agaccatgtt ggtgttgagg tcgttgcacg agtagccgag cttgacaccg
    29641 caaggcgcgc catactcgat gcccaactcg cgcccaggct ttccagtcgc aggcttcgta
    29701 tcgtacagtg ccgacacctt tatgcccttc aacactgaaa catctgcatc cacagaggct
    29761 ttcgcaatat gcctggtttc cgcaagtgaa aaatcaggca tcgctgcatt atagctggcg
    29821 cttatcagca tattgggcag cacctcgcgc tctttcatgg ctatgccgag cgtagtggtg
    29881 ttgtgggtct cgatcttctc tcccatggtc tgtgtcacct tgtggccggc tgtgagatcg
    29941 aaccccgaga tagcagttcc taccgtggcg ccgatggaac gcccgtcatc gagcttgccg
    30001 tcgtcgtcat cgccgagaat ctcgccgtta agagccacgt cgaatccggc gtatgatgtg
    30061 gcaaggccag cggtgacagc ggctttcccc tgctcaccgt cgactgggtt gccctcttcc
    30121 atccacctgt aagtgggatt gaaggcaagg tctgtgctgc gataaccagc cgtggctatc
    30181 acatccagcg gaagatatgg aaccttagcg gccgcattgg ccctgattgc cgctgccttc
    30241 gtattggtct gtgcagcata ggttgcgtca agcgcgaggc ccggaatcgg cgtcgcaatg
    30301 gcgtaggctg cgtaatccag ttcatcgtct ccgaccttca ctgctgaagc atcaacagcc
    30361 acgttggcta ctccagccgc tacgcgagcg cccctggcgc ggattccctt gtcgaatccg
    30421 tggaacacgc cagcctcgac aggtccgtaa gcaatgccag aaactgacat cccgttcaga
    30481 gcctccgtag agagtatgaa cggagagtat gcaagttcca gatctccaag cctgcttgtt
    30541 acagctggac catcagtcca gtaaggaccg actgcctcga gatacgccct tcttatcgct
    30601 cccggtactg gatcaccgtt ctctacgggc ttgggcgcca tgccacagcc gttcttctca
    30661 gctatcagcc acggattgaa ttcaatcaca ccggtgatag tctggccgcc tatgggccca
    30721 acgttcaagt tgagtttgag attcgtctcg gcgctgggag tatcggcgcc aaagtcatac
    30781 atcatcttgc tgtcgagctt cccgccgaca ctgacgttcg cggctgcgac aggcagtgcc
    30841 acggttgcaa tcatagctgc tgcaagtatc actgcaagct ttttcatgtt tgttccccct
    30901 tggttacttc gtatagtgcc gcatttgctg ttatgatgtg ttcgtctata gctgcgtctc
    30961 attgttgtgg cacatatagt tgctccgatg tgtgctgcgt ctcgctgtta gccggggaag
    31021 tgtccatgtt gccttcctct gcatacacag cgttgctgct tagtatggcg ccctggaaca
    31081 tgggctttcg agctatcggg ggtgcacctc ctttctcgcc cctcaccatg gcaaaggcat
    31141 catattgcgg tcgcgaacag gcggggtcgc ggtattcgcc tcggcatcat cgcctgttct
    31201 gcaatatcaa ttctagtttg tgagcgttgt tcctgcaggt aaaggcgggt ttgaacaggt
    31261 gtaagttgtg ggtacggagg tatctgtgac atatattgct gcgtattggc aaaattcttc
    31321 gcgtcaactg gtaaaaacaa gtctcaaaaa gtgcttgacc cctcttcggc agctagggtc
    31381 tgagccatac gcaacaaaga ctccttgggc acgcagagga tactcgggaa ttgctcggca
    31441 ccgcctggtc tcctgttgca gcgctgggga atatgacaca attgccgtta cgctgacgga
    31501 gcaaagacgt agctggaaat cgtcaatctt tgaagcaagt gtcgatgatc tcggaaatac
    31561 cttgaggaag agaacatgca gcagggcctg tgcgttctgc aaataatgaa aagggctgag
    31621 cgtccggttg accagaaaga cagcgttgta ccggttcttt ggccttagaa tcggcgcaag
    31681 ccaatcacac gatctgaaac agcccaggca acccctggtc aagttctgcg ctatcgccac
    31741 actcgcatcc cacctgtcgg gctacagcca tttgggggtg ctcaaatgct ccacgggtcg
    31801 tttcgcctgc aacgggagat ggacctgacc cctacccagc gtcagcacgg ttttctgcag
    31861 ccgtcccacc ctatctcgtt tggagcgccc tatccaaccc gggccgccac ggtctcgccc
    31921 ctacgacaaa gcgcggaccc ctgctacggc gatagggatc cgcgccccga ttatccttct
    31981 caaacaccag atcagctcat aaccaacgca agtatggctt tctgaacgtg caatctgttt
    32041 tcggcttcgt cccaaatagc cgaatgagac ccctccataa cctcatgggt gatctcctcg
    32101 ccacggtgtg caggaaggca gtgcagcaca atacaatcag gattggctgc atccacaatt
    32161 tcctgatcga tgcagtatcc ttggaagtcc ttctttctct tttcctgctc cgcttccatc
    32221 cccatgctcg cccatacatc cgtatatagg atatcggctc ctcgagccgc ctccttcgga
    32281 tcgcgcacaa cctcgagcct agcaccggtg gcaactgcat cggcctttgc agcctccatt
    32341 acggcagcat cgggctggta ttgatcgggg catgcgcacg taacatgcat ccccagtttg
    32401 gcgccggcgt acatcaagga atgaaccata ttgtttccat cacctatata cgtaagcttg
    32461 cggcctctga gatccccaaa tctttcgatt ccggtaaaca catcggccat cacctggcac
    32521 ggatggagca agtcggtaag tccattgatg acgggaatgg atgcccattc agccaattca
    32581 acaacttcat cgtggccaaa agtcctgatc attatggcgt caaggtagcg cgacaacacc
    32641 cgcgctgtgt caggcacagg ttcaccccgc ttgatctgaa gatcgtttga actgagaaaa
    32701 aggccataac cgcccagctg ccatatggcc gtctcaaacg agaccctcgt tcgcgtcgat
    32761 ggtttcaaga atatcatgcc taacgtcttt ccctcgagga gatgatggcg cacccccgcc
    32821 ttctgctctg ccttgagaag cgccgacaat tccatgaccc gcattatctg ctcactagtg
    32881 aaatcatgaa ttgataggaa gtccttccct gaaaagctgc gcccaagctc gtcgtagagt
    32941 agttgatgag cctttgtcat ctttccaccg ccttcaatat ttgtttccgg cgctctcgcc
    33001 cccagcaagc ctaacaggaa tcttgtggcc acatttggga cacgaaactc cttcaagtcg
    33061 aacagtgaca caggcgaatc cgcgcctctc tattagtagc tctccgcagt tggggcagac
    33121 cgtatcgctg ccgccaggga tgctggcatt ccctacatac acataattca gtcgtctctt
    33181 ggccacctcc acacatttct ccattgttgc catgggcgtt ggcggctcgt tcatcctata
    33241 gctaggaaaa tacctcgata tgtgcaacgg aatctccggc gaaaggcttg caacccacga
    33301 agcaagttgt tccatctctt ctggagaatc gttatagcct ggcactacca acgtagtcaa
    33361 ttcgagatgg caacctgatt cgtacacagc acgaatcgtt gagattaccg gcgcgagagt
    33421 tcctccgcac actttccggt aatactcatc atcgtatcct ttgagatcga cattgatcgc
    33481 atcaacgtaa ggcaatattc ccgccaatgg ctcagggttg atgtagccgt ttgtgacgag
    33541 gatattggaa agcccgatct cgtgcacgag tttagcgcaa tcatacacaa attcgtacca
    33601 aacgaaaggc tcagagtagg tgtaagcaac accaactgac tgctcccgca tgctttctgc
    33661 caagtccgcc aactggtcgg gagtaaagaa tctggattct gcgtgtcgtt gcgatatttc
    33721 ccagttttgg cagtatctac atgacaggtt gcaaccgaac gtccccacag acaagactcc
    33781 cgagcctggc atgaaatgat acagaggctt cttctctata gggtctatcg ccatagaagt
    33841 gatagcgccg tagttcttcg gaatgatccg tcctccgatc atggtccgaa cccggcatgc
    33901 tcccgttgca ccctcttgaa gcttgcatct acggggacac agctcacaaa taccgagcga
    33961 atcttctacc ctggcatgca tagcctctct attcgtatct agtcacctca aaccgctcaa
    34021 gttctacagg cgcattcaga cctataccgg ccttacgccg cgcaatagag atctgctcct
    34081 ctactgtgtt aatcccctcg aggtttggaa ggagaagccc cacccgtccc ccattcctca
    34141 caatgacccc atatctttgg ggatccagtt cctcaggacc ggagacaggt tccggaggag
    34201 aaagaacgtc cacagagtat acaatgtcat ccaactcgtg aggagcaacc ggctcgaaac
    34261 gaggatcgcg cacagccgcg gctatggcat tgaagatgat ttcctctgca atgtttcggc
    34321 gcgtcggctc tgtagtccct atacagccgc gcagctggcc ccttttgtgg agcgtgcaga
    34381 acacgcccgc cctcccctgc atgccttcag ggagcggatc aggaggttcc acaactacac
    34441 ctctgcgaac atagtcctcc acagctctcc tagccaatgc tgcaaaagct gactccgagt
    34501 gcctcagctt cctcgttttc tcttttccgc cttcaacaag ctcatggaaa cggcgggctt
    34561 cctctgatct accctttggg cgcagggcaa cgacagcata ccctacccca aaagggccct
    34621 cataggagag cacttccgat tccacttcaa taccgtccag cgatcctagc atcatgataa
    34681 tcggattgag gccgcacatg ccggcagcct cgattagtgc atcatcgagc cgagctaggg
    34741 ctgcaacgtc catggtccca agggcgccga ctaccgcctc gtcgaagatc ttgccttcag
    34801 gggcgtatcc tgccggtgca tccgggctta gccggtgcga caggtcaccg ctggcaatca
    34861 cggcaacgcg gtattgtgac ttgtctatgg cagaagccat gcacatgccg aacgtgtaaa
    34921 gctcattcaa tgcgcccaca cccatcgata ctgcaaccaa tctgaacgga taatgttcct
    34981 tagcgagaaa atacagtggg acaaccgcgc catggtcgag ccctgacggc accctgtaac
    35041 gcctagcaag cgccggctca agtacggcca cagaaatacc tgcggcgtgc gcttcatctt
    35101 cgatggcttg cataagctct agattgctgt aaaagtctaa ctcaacatcg gcgccgaatt
    35161 gggccagatc gcctgaaaga tgcggacgac catctatggc tatagcgtct gcaaataccg
    35221 gagcatgagg cgagatcacc accaccgtgt ccggcctcgc tgcagcgaac tccctggcca
    35281 actcctccat tgcatcgatg gtcttctgag cctttgcagc ctcacgccct cccactcccg
    35341 gaacgattat tgggggatgt ggagcaagag ctgccaggac tagttcaccc atgtctcatt
    35401 ctcctcccct gccgcgacac acctctgaga atcctgccgc ttcacacggg cgcccagaga
    35461 attcatcttc gagcagaggt catcgtgtcc gcggtcgata ttttccgccc cagagatcac
    35521 tgtttcgccc tccgcagcca accctgccaa gatcagcgcc gcacctgttc ttagatcgcc
    35581 agcatgtacc ctagcgccag tgaggccagg taccccctcg actatgatgc tcctgccctc
    35641 aaggcgaata tcagccccca tcctcttgag ctcgtccacg tggaagaaac ggctttcaaa
    35701 caccgactca gtaatgacgc tcacccctga tgcgacggac aggacagccg aaagctgtgg
    35761 ctgcatgtca gtaggaaatc ctggataagg aagggttttt gtgtcgcatg gctgtatcgg
    35821 gccatccatt gctgcctcaa catagtcatc gccaactgtt atggaagcgc ccatttcgct
    35881 aaacttggaa agcacagact caagatgcgt agggatgata ctgccgactt taacctgccc
    35941 atgggtgatt attcccgctg ccagaagagt ggcggcctca atcctgtcgg gaattacgga
    36001 gtaagcaact cctctcaatg aatcgacccc gtcgacgcga atcgtatcag tcccagcccc
    36061 acgtatgttt gcgcccatca aggtgaggaa attggcaaga tctacaattt caggctcttt
    36121 agcggcgttc tctatgatgc ttgtcccatt tgcaaggcaa gcagccatca tgatgttttc
    36181 tgtggcgcca acgctgggaa aatcaagata aactctagct ccttccaggc gaggagcctt
    36241 ggccacgatg tatccgtgtt caacctcgat gtcagcgcca agggcgcgga gccccttaat
    36301 gtgcaagtcc ataggcctcg atccaatact gtcgccgcct ggcaatggaa tgctcgcctt
    36361 cccctttctt gctaggagag ggcctagcaa aaggctggaa gccctgagcg tcttggcgag
    36421 ctcatacggt ggttcatgat gaatcaagcg atgtgtgcga acacgcagag ctgaggggcc
    36481 cgccctccta atactgaccc ccatctctcg aagcactgcc gacatcgcgg agacatccct
    36541 ggcgccaggc gcgttctcga taatggtatc gccttcagcc aagagtgatc ctgccattat
    36601 cgcgattgca gcgttcttgc ttccaccagc gcgaaccgac cccacaagcc gcttgccacc
    36661 tgctataact agttccacag ttccgacctc cagggcacct tgcatttcca atgcgcgcct
    36721 atgaatagct attcttcatc cggcgccttt tatccttgtg ctccaacttc accttgacta
    36781 cgttctaaaa tggacaattg acaggtttcg ttccagaggg ccctctatct gcatatggcc
    36841 cgagatagtc tggagaggct tcccctctac caaaatctca accttcttta catacgaaaa
    36901 ctcggtcagg gcattgacaa tcgagtacac cgtcatctcc tctgaacgac tgccgcccca
    36961 gtgattcatt accagctcag cgctgaagtc ggccacagct gtgtctccct gaagtctaac
    37021 tgaacgcaat accgtgcccc gcggaattgt cggctggagt atgctgtcct tgcccggccc
    37081 tgctattgtc gctcgcacaa ctgcggcgag ggcttcaatc ttgttgaggc ttttcagtcc
    37141 cggtatctcc ctggcctcag gcatcagcga atcatcgaga ttcgtggggc caccaaagta
    37201 gaggatggtg gaatcaggag taagctgaga tgtagcagtg cctactttcg taacaaggcc
    37261 ccatgcttca tccgaatcat cgtctggcgc aagaacattg cctgcttcac cctggctgtc
    37321 catgaaatag gccataattc catggaatac tgcttctgca actttcacgc gataggcggc
    37381 atctgcaaga agcctttcct cgcgcgggtt cgacagaaac cctacctcta caagggcaga
    37441 aggcgccttt accttcttga gcaagtatat gtcggctggg cgagccttcc gagtgttggg
    37501 tccgaggcgg tttaccagct cagtctggat atgtacagcc agtttcctgc cctccgctga
    37561 cgcggcatta tagaagacct gtgcgccgga ccactgcggc tcaggaaagc tgttggtgtg
    37621 tatcgtgacg aatatgtctg gagagttggc gttcacgaga ttcgcccgcc gttccagctc
    37681 catgcgcttt ctggatcctg tgatgtcctc tccgtctgcg atcaggccat ggtctgattc
    37741 ccgggtcatc acagtataca cagctacgcg ccctagcaat tgccttagct ccaatgcgat
    37801 gtgaagattg atgtccttct ctatgattcc actttttccg cgggcgccag aatccgggcc
    37861 gccatgtccg gcgtctatag ccactgtctt tccggagacg acatcgcgga ccggcgccaa
    37921 tgtagtgaca acaaggacct gcacagcata gatcacaaca ccaatcagca ctgctatacc
    37981 aatcaagaca gctgtgcgca ccggcaacgc gataaagatc acaggtcttt tcatttcgca
    38041 ggcctccagg cggcaaggct cgcgacttat gcggcctcag ctcctaaaca gaatatgccg
    38101 gaggcaccct ccaaatgacc tgtgtccgat atgcctaagc tccgtgctgc ccacagcttt
    38161 tgtccggcct gacaacctgc tccaaaatgc gcaagactga ctggtcactc cctgaaagcc
    38221 cttatcactc tatagccccc gcctatttca ggctcatcag cgttgccgta ggtttgagca
    38281 ataaatgctt tgagagaagc agccccctgt ttggtgcgaa ctactgcgta gaagcttcca
    38341 ccaggctcaa ggtgatcatg ggcctcttca atcaaacgat acaggaccgc ccggccggcg
    38401 cggataggcg gattggtcgc tataacatgg aacatcctgc cccgaacagg gtcaaaacca
    38461 tctcctgaca caaccgtcac gcgctccact ccatttctgc gggcgttggc ccgggcaagc
    38521 tcgcaagcgc gttcattgat gtcagtcatc catatctcac attccggatg aagtgaggcg
    38581 atggagatcc caacaactcc gtaaccgcat cctagatcca agagggactg acccggacta
    38641 atccggatag cctgtataag ctggtctgtg cctcgatcca cacgagtctt cgagaaaacc
    38701 ccggcatccg tttcaaagct gagttccctg ccacctctcg gagtataggg tatgattcgg
    38761 cgctcgtgcg gagatgtggg tgaggtcgtg aaatagtgtt cagacagtgc acacacctcc
    38821 ctgttttagg ccttttgggc cacacaggtc tgagtaattc cacaggcctc acccgtcttc
    38881 ctttcgccag tcccatatgg cgcgacttaa gccgtgccac tacatggtca gactcccctc
    38941 tgatgtggta atgagctgca gtactttctc ctcggttcct ttttcagcat tgatgtaaac
    39001 aaaaaactgt tccccatagg atgtacccct aaactcccag cagaacgcct cgggcgtgtc
    39061 aactgtctcc actgggatca ccgcaagacg ggccggctcg gttaccgtaa gcgccggact
    39121 gaggagttcc cgtgcctcat ctgcatctat cgaaggtttg ggaagtcggc gcatagtgtg
    39181 ggaggttagg taggccaatg catcatagga gagaatatca ccattgtcca aagccacctt
    39241 cactttgacc tggtccggat agatcaccac accatcctgc gtgtatacga acgggacaat
    39301 agagacatca ccaacctcag atgcaaatgt gggagtcata ttgaccattc caactttgct
    39361 taagaaggcg ctagcacgct tgacagactc agccagatct atcgtctttt tgtcaagatc
    39421 ccgcgccacg ttcatcagca caacgtgtcc gcccttcttc gtcacgtcaa caactgcgtc
    39481 cggctttccg ctcctcttcg gggcgtcaag ccgcaccctg taaatgtcaa tgtccccttt
    39541 ggcctttata ttctcttcca ccgttgcgga atgggatgag gcgtcaaatg gaacgaatct
    39601 gcgagccacc tctttggcct gatcggcggt gatctccgcg ccagtgattc cccttggcct
    39661 gatctgctcg atatgatccg agaaaggtcc gtcatatatc aaagtcggga aagtctgaac
    39721 ctgttcgtcg actctagtga agccgtcctt aaccacatct ggagtagcgc ttgtgcctgg
    39781 tttcggtttc acttctgatt ttgcctgttc ggctactcta acccaattca ccttacctgt
    39841 agatacctgt gcctgcatgt cggcgagtcc acgcgccagg tcttccgatt gcttcttcat
    39901 gtcgacaagt ttgttccaat catcctcgct catcacctgc tggttcgcta ccttgcgcgc
    39961 agttgtaaac gcaaagtcgc ctacctgtgt caagaaggct gaagtccgcg ccaaaacctt
    40021 gtgggttaca ggaaggttgt tcagattcgc ctgcgcattg ctggcctccc gccaaacatc
    40081 agagagcgca cccacgcgga gtctgggaga accggatgca aggctcttgg atagcgctac
    40141 tcggacattg tctacatttc ccagaagatc gaagaatgac cgaaaatact ggttccccac
    40201 cgccacttcc agcctttgct tggcggccca ttgcgaatag gcaaacacaa ggctcacaat
    40261 tacaattact agtaggagcg cctgtggcca attgcgcatc ttcccgcgcg ctccgcttgt
    40321 cccatcggtc atttgcattg tcccctttct acctggcaaa cacgtgtttg cctattttta
    40381 cagcaatgct tctgctccat atccatcctg atacgggttt ggcagggttc cagaagaata
    40441 cgcacccata tgtaggatcg taaccgttca tagcatctct tgcggcagat atcgcctgtc
    40501 tgctcggaga acgcctccat atcaatccgt ttgagacaga ttcaaatgct ccaggttgga
    40561 atacacatcc agatattgaa ttgggaaatt tgggactcgc cactcggttg agcatgacag
    40621 cgccgacagc taccatcccc tggtatggct caccttcagc ctcggctgca atcaggcggg
    40681 caagaaggtt gacgtcgccg ctacggcttt gagctcgacc aatatgcgca accgttccaa
    40741 taacgacggc aactgcaagc actacaaaga caaggatccg tgcgttgggc attctcatgt
    40801 tggcatacct ccttccgatc aaaaccctct ttgcatcagc gggcaaaaac atgcttaccg
    40861 attttgacaa tgatattccg agtccaaatc catccagata ccggctttgc ggggttccag
    40921 aagaaaaggg atccgtaggt agggtcaaac ccgtttatcg catctcgagc ggcgctgtac
    40981 gcaatttgat ctggtgttct tcgccagtac agcccgttgg acacagattc caaggcattc
    41041 ggctgaaaga tcacagctga cagcgagtgt ggaaacttgg ggtttttcaa tcggttcatt
    41101 agaaccgccc ccactccaac ctggccacgg taaggctctc ccctggcctc agcggctatc
    41161 gcgcgggcaa ccaaatctac cgacgccgac ctagatgcca ttggcgccgg cttgtaggcc
    41221 gcgggcgcct cagcagccac cgtgtagccg aagccaagag ccctccatgt ctgcggtcca
    41281 acgaggccgt ccaccttcag cccgtttttg cgctgaaatg ccaaaactgc tctgtgggta
    41341 ttggcgccga aaacgccatc tacccaccct cggtaataac cccaatcgga aagcttccat
    41401 tgaacgagtc taaccgaacg ccctctggaa ccccagtaca gcgtcggcct tgtctcggct
    41461 ttagcagggg ttgcagcgca taaaagcacc acaatgaaga tcatggcgcc tacagtcaaa
    41521 cccataatcg gtgttttcag gttcatcgat cagttacact cctgtcctgg ttggctcgca
    41581 caacgtgctg gtattattct ctcagcactg ttccatggat atgcacggac aaagacaagc
    41641 cggaagctaa ggctgaaacc gcaatatagc gcgtgcaacc acgctagagc atgaaaaaca
    41701 atgtcatgcc tcacatgatc tgcagaaacc ctcgcctagg tcccctggtg attgaacggg
    41761 ccttccagcg ttctggacgt aggagaatag tggagatatt atcagcttga cctaggcaaa
    41821 gacttgatga tgcggaggat ggtcattaag aatgatgcat acaatagggg ttgtcttcaa
    41881 tctgaagaag gacttccccg gccttgtcgg gaaagcaaac agcgttcccg atctggctgc
    41941 ggaatgcgag gaagaggaga ctgttgacat gctctcatcc tctctggcca agatcggtga
    42001 gaaggtgatg ctgtttccgc acaaatctga ccttttggag agattggacg aggcgcggcc
    42061 tgacattgtc ttcaacatag ctgaaggctg ggctgggcgg aatcgggagt cccttattcc
    42121 ggcactgctt gagctgctcg agatcccgta cactggctcc gatgccttaa cgctcggcat
    42181 tgccctcgac aaagccatgg ccaaacgtgt ggcatcgact gtaggtgtca ggactccccg
    42241 ttacatcaaa gtcagttcca tagaggatct gtcctccatg aatatggact ttcctgtatt
    42301 cgtcaagccc aactacgaag gatcgagcaa gggaatacgt aattcgtcgc gcgtcaggaa
    42361 catggatgag ctgactgaaa aagtcaggtg gatactggag acatattcac agcctgcgat
    42421 tgttgaggag tttcttccag gaagggaatt caccgtcgga atcatcggaa acggacgacc
    42481 acacgtcctc cctataatgg aagtatgccc cggcaatcgt gactcagccg attgcgaatt
    42541 cgtctattca tacgaaacca agtcaggaaa ccttgagcgc ttcagggttc ctgcgcctat
    42601 tccagagcac ctagcatcgg agatcgcaga tatggccgtc tccgtattcg aggtaatgga
    42661 atgtcgggac gtggcaagga tcgacttcag gctcgatgca gaagaacgac cgcatttcct
    42721 ggagataaac cctctgcccg ggctctccaa agtcagtttg cttccgcttc aggcccgggc
    42781 ggctggaatg acgtatgacg atcttgtgaa gtggattctc acatcagcct ggtcccgcta
    42841 ctggcactag ctggccaatc aacatggcgc tttcacaagc gaaaagagcg cgttcgtatt
    42901 tcgctcacag cgatgcgaac gtgctctttt attgtgttac aaagcgcccg ttcccctctg
    42961 cgcttattac cccgttccat cggggcatac taacgcaggc atttcatagc cagtctcttt
    43021 gagaggagaa aagaacaggt atggataaca gggttaggtg ttcggtaaac aactgccatt
    43081 actggaatga aggcaactat tgccatgcat cacagatcat ggtgacgtcc gattcaatcg
    43141 caaatcaact tccagacagt tacgacgcgt tgcaagcttc catagctgag ccaaggccgg
    43201 ctgcccacat cggcgagact tgctgcaaga cattcgctcc caagggaacg tcagatacga
    43261 cggtacgctc ggatcgaact actcgaatgt aataggagtc cctgcgttac accgggcggg
    43321 accgtgcacg gtcccgccca actcgcatac gaatggttgc ccacgctaat ggctgacacg
    43381 cttttcgaaa ctgtgtatcg cctttacatc atgaggaaat gcccacagat agggctgaac
    43441 caaaacaggg ggcacccgcg ttctaagtag gtctcgataa gtttctgcgc gaccttggag
    43501 tctgtactgc ctgcggttca aatctagact gcttgatttg acaaagtgat tgctcaggta
    43561 tgcgctaaaa ggcatttgag cgctccctga agcagccaaa ccccttacgc caaatagtaa
    43621 tcagtcgcgc aaacgccgcc ggaagactga gctgaccttt gaggcccgga caagaagtgt
    43681 atggcgatgg ccaaggtttg ctgcgaggaa aatcagaggt tggcaacgaa cctggccaac
    43741 ttctagtcac atccaattga ggcgaactag ctgatacctt tttatggcta tgttcactgc
    43801 ataaaccgac cctcactcgc aaaaagcagt ccaacgcgtg tcgatagagc tactccgaat
    43861 aagcctgttt tcggcaacaa ttcaatctac gcccaccaag cgacttcgaa ctgagcttta
    43921 tacacgtacc cagatgatgc tttcgatgcc tacaatgtga tcaaatgctt ctacgtcgaa
    43981 aagggcttac atcctatagt ccagcatgcc tccacacgtt caacgccaca aacgatgcaa
    44041 tgtgacttga acgctcacag aacttggctg agcgcctcag ccgtaaccga acccgacttt
    44101 cgcgaagacg cctacacatc tgggtggttt attcggcctg cccaaacttg tctccggcac
    44161 ggattcgcac ggctgcctaa gcctggcagc ctatttgaga tttacctttt gagatgcttc
    44221 tcacagactc gttggagtcc tggcgtcccc atacacgccg gcaattgcgg agtatctgga
    44281 atcatctagc gaagacgtga ctgagttgat gcaagcgcta gaggatgaaa ggggtaatgg
    44341 caggcgtcgc ctctcaacat aagactacca acggtcttcg tcagagctaa tgaaaaacgg
    44401 acagctccgc caggtttgca atctcgacat tacgctgggc gtggaggaag tcccctgatc
    44461 atcggcgcac accggatctt tttgcaatct catgaagcat taaagagagg ttgacgagat
    44521 tttcgataac tcggtaggat aataggcctt atacatgctg tatagaagca gcctgctgcc
    44581 gcctatctcg gcaaatgagg ctcctttatt gccaggaagg aagttgctct cgctaaattg
    44641 tgtaactggc atgtgcgaga aatccgctac aacgcagatg cttcgggatt ttgcgctctt
    44701 tcaaccgcaa tatctgtaga gtataatggt atggtattct atttatgagc ttcaagagtc
    44761 gggggggaag cccatttgaa gcggacagaa cacttgaagg atttctttgc caagtactgg
    44821 tggcgctatg tcttaggaat actcactctt attatcgtcg acgctctgca gcttgtggtt
    44881 ccaaagatcc tcggaaatgt tactgatgca ctccgcgaag gttcccttac accggaaggc
    44941 ctcaaaaaac atgttctcat tattcttgca atagcgtgcg gcatagcaat aggaagattc
    45001 ctgtggcgca tcttcattat gggcactgca aggttgctcg atttcgagct acgcaataag
    45061 ctcttcgccc accttcagaa gctgcccgcc cgctactaca ccgagcacaa gaccggcgag
    45121 ctcatggcac atgcaacgaa cgatatccca tcggtgaggc aagccttcgg gcaaggcttc
    45181 atactcatta ccgactccat attcatgacc acggcgacac tgataatcct gttcagaacg
    45241 gtccccattc gtctggcaat cttcgctttg cttccatttc cgctgttggc tgtgtctacc
    45301 gcggctttta gcaagatcat aaatccacgg catctagctg tgcacgaggc ctttggagaa
    45361 ctctcggatc gagctcaaga gagcatagca ggcatacggg tcacaaaatc ttttgtgcag
    45421 gaagacgctg agattgcgaa attcatggct gtagcccaga agaatgttga cgcaaacata
    45481 gaccttgtga agatatgggg gctcatgtcg cctttgagcg gcctggcaac tgcgctcgcc
    45541 tacgcagttg tccttcgata tggttcgtca atgataatgt accaacagat ctctctcgga
    45601 gattttgtag ctttcacgag ctacctggcc atgctggtat ggccaatgat agcgcttgga
    45661 tgggtcataa acgtcatgga gcgcgggttc accgccctcg acaggcttaa cgccgtgctg
    45721 aacgaggtcc cggaggttgc ggatgagccc ggcgcaattg agctagagga cgtcacagga
    45781 accattgagt tcaggaatct gaccttctcc taccgagacg gggtgcctcc ggcgctctcc
    45841 gatatttcct tcaagataga cgcaggaaag accctggcag tagtgggccg gaccggaagc
    45901 ggcaagacga ctatagtcaa cttattgacc agactctaca atccacctga aggttcagta
    45961 ttcatagacg gtcacgacat ccgcaacgtt acactgagct cgctccgcaa ccagatcgga
    46021 tgtgtgccgc aagacacgtt cctgttctcc tgcacgattg gggaaaacat cgccttcgca
    46081 gatagggact tctcgcccga ggagatatgc cactacgcaa agatagcaca tgtatacgat
    46141 gacataatgg gcttccctga aggcttcgac acaaaagtcg gcgaacgcgg agtaacgttg
    46201 tcaggcggtc agaagcaacg gatctccata gcgcgggccc ttataacaga gcctaagatc
    46261 ctcattcttg atgactcgtt gtcagccgta gacactcaga cagaggagag tatacttcgt
    46321 gggcttagcg aattcatgca tgatagaaca agcataatca tctctcaccg catctccact
    46381 gtaaaggatg ctgacgagat aatagtgctt gatgacggca aaataatcga acgtggaacc
    46441 cacgcgtcct tgctggccca ggcagggctt tacagtgaga tctatgagaa acagctgctg
    46501 gaagaggagc tggccagcag aagctgaccg ggggagaggt gagatgtaag tgaacggtta
    46561 tgaggaagag gaacaaatca tcggaaaagt gtacgattca cagcttatac ggcgactaat
    46621 gtcgtacgcc gcaccatacc gcaaaacgtt cgtgctagga ataatagtta tcatgataat
    46681 cgcgggaaca gatctagtca ggccatacct tatcaagact gctatggacg attacatctc
    46741 tggatacgat gcccctatgg ctcgctatga acctgaggcg gttcccgcag gtattcgaga
    46801 taatcgcatg atacattaca atgggcacgt ctacgtccgc atcaaagact tgcccagcaa
    46861 ccatgcggat gaatacgagc cggaagccct tttccagctt ctgaacatct ccgatcagta
    46921 tgttctcata tctggagcgc tcccgccgga tcacggcccc gctcaattgg caacaacgga
    46981 ttcaggccta gtgctcatcg tagacgaggt cgaacacccg gcccaaataa tcgaaggcca
    47041 cgagaatatc ggcatctttc gggagcaaga ccttgccgca gtatcaagat tagccattat
    47101 tctgctccta gtcggcgccc tggcattcgt cctcaactac gggcaaacag tcctgctgca
    47161 acgagccggc caacggataa tatatgaaat tcgcgaagag gtattcacac atcttcagca
    47221 catggatgta gcgttcttcg acacgaatcc cgtgggccgc ctggtgacca gggtgacaaa
    47281 tgataccgag actctcaatg agatgtatac cagcgtaatc gttaacctct tcaaggacgt
    47341 gttcatgctg gtcgggataa tcgttgtgat gttccgtctc gatacgcagc tcgctctcct
    47401 gtcccttgca gccttgcccc tgataatcgt ctcgaccatc atcttccgta ccagggccag
    47461 acaggcatac aggcaagtcc gaactgcgct ggcgcggatc aactcgacat tggctgagaa
    47521 catctctggc atgaagatcg ttcagatctt caatcggcag caaaggaact atgacgattt
    47581 cgtaaatgtc aatcgcgaat actatcgcgc gggcattggt gagatgatgg tatatgcgat
    47641 attcagaccc ataatggagc tcataggaac gggcgcgcta gctctggtcg tatggtacgg
    47701 cggcaggaac gtcatctcag ggactatgca gtttggcatg gtttatgcct tcatcaatta
    47761 cgtcagcatg ttcttcggcc ctataaacga tctgaccgag aaatacaatc tcatgcaagc
    47821 ggcgatggca gcatctgaac gcctattcca gctgatggac accgagatca tggtaaaaga
    47881 ccccgagaat cccgtttctg ttgatcggtt ggacggtcga atagagttta agaatgtgtg
    47941 gtttgcctac aacgatgccg attgggtgct tcgcgacatc aacttcacca tcgaaccagg
    48001 cgaagtagtg gcgttcgttg gcgctaccgg cgcaggcaaa acctcaataa caagcttgat
    48061 aaaccggctc tatgatatcc agaaagggca aatactcata gacggagtcg atattcgcag
    48121 ttacagggtc gcagaccttc ggcgcaatat cgcagcagtg ctccaagacg tgttcctgtt
    48181 cgctggagac ataaagagca acatcaggct gaacaacgtg gatatctcag atgaaaaaat
    48241 caaagaagtg gcgcgctacg taaatgccga ccatttcatc tccaaattgc ccaatggcta
    48301 cgacgagccc gtcactgagc gcggctcgac actgtctgcc ggccaaagac agttgcttgc
    48361 gttcgctcga gccctcgcct ctgatccgtc aatactcgtt ctcgacgagg cgacagcaaa
    48421 tatcgatacc gagactgagc tgctcataca agacgctttg ccaaagctga tcgcaggccg
    48481 cacaacgatc gtggtcgccc acagactgtc gacgatccaa cacgccgaca agatcatcgt
    48541 gatccataaa ggccgcattc gagaggaagg cacccaccag gaactcctcg ccaagcgagg
    48601 gctatactat gatctttacc gcctccaata caaggacgaa ctcatgccag agcccgaatt
    48661 ggatatgcag gacacctcag ccgccggttg cgccaggact gcaggaatcg ccagctccga
    48721 ttcggctgat aagactgacg acatggcata agcttggcac acatacgacg ttcgggactg
    48781 ccacgacgac cttctcccta tcctcgggtg taggcgccac caatacggca gtccctgctt
    48841 cacggaacat gcctatgtcc ccccagttgt caccgacagc ccacgtctcg tctgtatcgg
    48901 ctccgagctc cattttgagc cttctcaaga cagcaccttt tgtcatatgc ggttcatcaa
    48961 ttgaaacatt tatcactaca tcacctgtca tcacaccctc ccttgccacc agctcgtttg
    49021 cgaaaacgcg atctatgcca agatcactgg cgacgcgtcg agcaagcgct aagatgccag
    49081 acgatatcaa agccgtgaat gctccatgag atcgtgcggc atctactacc tcctgcgcac
    49141 atctagcata gtgaatctcg ccgagcgtgc gctcgatctc cgcatgcaac atccccttcc
    49201 attcctgcgc atccaggata gaaaactcct catatgtgat ttcccctgac agaaagcgca
    49261 tcatatgcgc ctcagcacgg cctgtccata gcccaaagcg gcgatgtacg tactcccacg
    49321 agcttggttc ctgtgtcagg gtaccgtcca tgtcaaacgc tattagcttc atgcccatcc
    49381 ttcacccaca ctccgtctcc atcaactgat tgcgcgtcga tctgaaaagc ccttatgcgc
    49441 ctgagtgaat cggcggccgg atacttctcc ggccgccatc tacatattca gatactgcac
    49501 acatcaatgc agcccgattc cgtcacactc agtccgcctt gaaattgaga ctacattccc
    49561 ttgcctggtc cggtctgagg taattgatac cagccttttg aatacatagt ctgccagagc
    49621 ccgcgttgct cattcatcca gtcatcatga ctgttcttga aggtttcgcg cacgctctgg
    49681 tttgacgcct ccactgtagc catgtgatat cccagcgcca actccttgag gtcactcaac
    49741 agatcagcag caatatccct gtcgctcagc ctgttctggt ccatagttct cctcctttcg
    49801 gtttcggacc attctaagat cattgcattg gcatctgtcc tgactgaccc acaagccccg
    49861 aaagggcatt gacatggcgt tggcatgact ccatcatcct ctgaacgcta cccttcagct
    49921 gcggatcttg gatctggttc gaatagatgc tacacttctt caaacacagc tgctccttgc
    49981 tgagctggtc ttgagcatac aagacctcgc gaggcgtcag cccttgctgt tgaaccaatc
    50041 ccgcaccccc ttccgatgga ataacggacg tatcgctata cgtatcatcc cgcgactggg
    50101 gcatgggtat tcgttggacc atcagtatgc tagtgggcag ggctgaaacg aaagttgaca
    50161 atgtcgccat cggccatcac atattccttg ccctcaagcc tcacactccc ggctgatctg
    50221 gcatcggcta gagatcccgc tgacatgagg tcgtcgcacg caacgacttc cgcccggata
    50281 aaacctcgtt caatgtcgga atgaatcttc cccgccgcac gctttgctgt ggtcccgttc
    50341 tcaatcggcc acgcgcgaac ctcatcttca ccgacagtga agaaagagac aagtcccagg
    50401 ctatcataga ctgcgcgcga tacctgctca ataccgctaa cctctattcc gtaagcagcc
    50461 atgaacagat ctttgtcgtc agaatcgagc tccgcaattt ccgcctctat ctccgccgcc
    50521 aatctgatca ggggtatgct tcgttcgcaa caatactctt gaagttgact gtctcgagga
    50581 aactttcctt ctctgaattg ctcttcactc atgttggcaa ctaccacaat gggcttcacc
    50641 gacagcagtc cgtatcctcg aagtcccgca gcttcctccg gctcgagctg gatatccctg
    50701 aggctgcgcc cagcgccaaa ctcctcgtcc aatcgctcca tgagggccaa ttccttatct
    50761 tcctcttctg tacgcttttt tgaggaattc aaccgctcta tccgggcttc tgcaatagcc
    50821 atgtcagcca ggattagctc gtcggtcagc gccgacgcat ctgcaagggg attgggttct
    50881 cccagctcca aggagtcgaa tgtgcggact acgtttacaa gagcgtcaac attcctcata
    50941 tggttgatga gcagtgttaa cccccgtgca atttcacgtt tgttcgagct aaagtcgtcg
    51001 aacgttgatc cagatgatgc ccttcctgcc aagtccacta cctctagctg tgcataagta
    51061 gtcttcttag gctgatacat cttgctcaaa agatcgatac gagcatccgg aactcgagcc
    51121 atcccgatgc tcgcatcctg tttgcgcgac ggggcaactc cggtcaggag acgaaatagg
    51181 gtcgtcttcc ccgctcccgg catgcccact atgccaattc tcatacaacc tgcaccttct
    51241 cgttgtctac tcgcaagcct cgtcctaacg aagggttgct catttcttta tccatttcca
    51301 cgatcctaca gaagaataaa acactacatc aggcgtcacg cattcttcca aaacaggacc
    51361 ctccaagaaa ctcccgcatg atggaattag gaggaatgtc agccctctct accggaagga
    51421 tgtacgcaag gaacttgcgc aaacgcttag cctctatata ctcgggatcc tcctgtccaa
    51481 tcgcggccaa cagcggatcg gcatatggct tcaacaccgc aagctcatag gctaacgccg
    51541 tattgaacat cacatcggca gtctcttgga aagggaaaat gttcctttcc tctccagccc
    51601 taaccttagg ccacatcctt atggtgtcct tcgcgctgtg cccacggaag agattgtccc
    51661 tcaccagcct cctcaggatc ctcacatcag tcgttggtat ccggttgtga tcatcaatat
    51721 tgagatgagt aagggccgat gcatatatct tgaattttcg gcccatcgga atagatctag
    51781 tcaactcatc gttgagcccg tgtatcccct caatgatgag catctgatcc tgtccaagtt
    51841 tgatgggacg acccttcgag gaccgcttgc cttcgataaa atcaaagaca ggaacttcga
    51901 cctcttcgcc ctgaatcagg cgggtaatgt gctcattgag caacggaata tcgattgcct
    51961 ctatggtctc gaagtcgtat tcgccctctt cgtcgacggg ggtattcgcg cgatcgacga
    52021 agtagtcgtc gagagagagt ggaacagggt tcagcccatt cactctcaac tggacccaca
    52081 ggcgttgagc gaaagtggtc tttcccgagg atgaagggcc agcaatgagc actaccgcta
    52141 tcctgtcccg gttctgagca ataacgtcgg caatctgggc aacctttttc tcgtggagcg
    52201 cctccacgat ttgaattaaa gcatcgatat tgcccgcgcg gatcacgtca ttgagggatg
    52261 ccacatcgtt tactccaagg acccgccccc atttctccgc ctcaaagtaa acattggcaa
    52321 gcttaccctg ctcaacgtac tcgggcaatt ccagcggatt gctccttctc ggcagttcca
    52381 agataaaccc tggcaaataa tgtcgaagcc tgaaagtgcc aaaatatccc gtgctaggcg
    52441 ccatgtaccc atataggtaa tcgcttagcc aaccgcattc gtatatgttg accgtgtcgg
    52501 ccttccgata ccgaagcaac cgagccttgt ccatctgtcc atctactctg aatagctcta
    52561 tggcatcttc cacgagcata cgctttctgt ttataggctt gtcggcctca acaatctccc
    52621 gcatccgctg ttcaatcgcg cgaacgtgcg cctcagtaag cggccttgtc catttgatct
    52681 cgccatacag gccgttcgag agcgaatgct gtatgattac acggcaatcc ttgagcactt
    52741 cacgggcggc catcaccaac acgaggattc cagtccgggt gtacaccctc atgccatctg
    52801 aatcgcccat gtcaacaaac tccacatgcg cgtccgtgtc aaggacatac gtcagttcct
    52861 taaggttatt gtcgacctta gcagccatca caggccagcg aagtccatgg ggaatgatct
    52921 tcgccagttc ctcaagagaa atgcctgaag ccacgttgtg ctcgctggcg tccggcaggg
    52981 ttacccttat cgaactctcc atggttatcc tccccttgtt gcgggcagac gtacctaata
    53041 tctttcgaca acgcagcgta gttccctcca taactacgac ttgccgagga acccgccatc
    53101 tagcgccgcc tcaaaagcct tgttgaaagc gaaaacgcct atcacgaagt atactcccgc
    53161 aagaattgcc gcggacgcca aatcccctcc agccacgaat tctccaaggc cgactccgcc
    53221 gaaagttatt tcgcgagcca tgtagtagca tcccccgtat ggcaggaatc tgctgactgc
    53281 agctactgaa cccttcttcg catccaccgg gacaaatgcc acagcgaaca gacccataga
    53341 gatcaagttg acaagtcccc ctattctttt tgctaggagg gcgatcccgc ccagaacata
    53401 gcctattccc agcattccta agcgcatcag gatcagcggc acgagcgcag acagttttgg
    53461 agggggcatg cgggcgcctg ttgtaagaga caccgccagt attatgacgg cgatattggg
    53521 caggaaattc gccagcgacg caatatcccg tataagggca atacgccaaa gtggaatact
    53581 agatgaactg agctgctcaa ccacgcctga ccccggctcc tccgtgacaa ttcgcaccac
    53641 tccctgcaag gcccccatac aaaaaaagac gcatgcaagg gaaaacagga gctcatcggc
    53701 agcgtccccg gaggtaacag cccgtcccga ggcggccata atcgccacag tcagaaagac
    53761 atatgctgcc agggtctcag taatcagttc cacaggactt ctgatctttc ggatcagtcc
    53821 cttcttgatc tcaacccata ccgcttgcac attcatcaga tatcacgcta cgtaaacccc
    53881 ctccttcgcc ttcaattaca gggggaagta gcgttttccc tcctctcgca cagcattggc
    53941 cgatgccgag atccttttgc catccaagtt gcgcaggtta aaacagcctg taacatgtat
    54001 ttccaaagat aaagcactcc tagcctttat acccaacctt gcaaaacagg ccaaacccgt
    54061 gaatataccc agctctcctg cgctcgctgc ttggtgatgt acccataggc ctccgtaaac
    54121 acttggatat aactcctgca gcattgttcc catgcacgcc acatcgtggc aaaccctttc
    54181 tgcgccatcc atggtaatgc tggttatcag ctgaatcatt aagggctcca tgattttgac
    54241 gagtatccgc atgcttcgtc ctctcgcagc aagttgccca tgaactcttt ggccatcacc
    54301 cgcagtgtct aaggcgcata tgccccagct tcaggcgtag gaaggttggc gaaaagagcc
    54361 atcttcttct ccggcaagtt tcaaaaacac agactccaga caaggttctc gcctggttac
    54421 agactcgacc cccactgctg ccaactccaa cacccccata acatcgaaga atccacgctc
    54481 gtcaggaaca tcaatatcga tcaccgtgta tccggaatgg cttacaccga agccatccag
    54541 ccatttttcc ctcacctcta cctgccccaa accgctcaaa gcggttcgaa cctcttcggt
    54601 caatgaccct gctagtgtca aggtataagc tctgaaagcg aagacgtcca gaaggcgcct
    54661 gactgtatcg agagccaaca ccctgccttc atttaagata catactctat cacatacatc
    54721 ctctgccaca tccatctgat gagtagtcat gatcacagta cacccttctt catgggccat
    54781 gcgcttgatc gccttgcgca gttccagaga ggacatgaca tcaaggccca cagtgggctc
    54841 atcgagaaga acaacccgag gctctgccgc aagggcaacc gccagggaaa ccttttgcct
    54901 tgtgcccatg gataggtcat tcacaggcct atcggccact tctgcaatgt tgaaaaagtc
    54961 cagccattcg ccggcgcgaa ttcgaagcct ctcagtatcc cgcagacctt tcatgatgcc
    55021 aaagtattgt atgttctccc atggcgtcat ccgagggtaa agggtgcgtg cgcgatccaa
    55081 taccacaccc acacagctca aagcctcggg cctttgctcc accacatcat accctccaat
    55141 aagtatcgtg ccggatgtgg gagtaacaag ccccgacatc atccggatag cggtgctctt
    55201 ccccgctcca ttgggaccaa gcaggcccaa aatctctccc ttgggaattg ccaatgaaag
    55261 atcgcagagg gcaacaacgg gcttatcgca tcgccgacgc ctgaatacct tgcgagctga
    55321 tttcatgtca acgaccaaat cggacaagaa taccacctcc ctgcggccat cactacctgt
    55381 aattgtaata tcataaatca ggatagtcaa ccatttaatg ggttaaccat ctggctagtc
    55441 ccactcgact agcattgcca taaagtcagg cgaagcgctg atatcggctg aaaatggcaa
    55501 gggctagaga agaagcagtc tcggggttga tcgctttacc cgagcaacgg tccggatccg
    55561 aaaaagcggc atcgtagcaa aaggacccta acaggcatga aaaaacccgt gcgcttccac
    55621 acgggtaacc agaacgacac gattggaatc aatatgggga gtcaggatta cgcaacttgc
    55681 cacaggcaag catgcctcaa cccacaaggt acattggagg aaacttgcag tcagctattc
    55741 ctcctctgcc cacaacctgc acaggttgac tactacttca gcagccttct ccatccactg
    55801 tacgcacgcc cactcgcgcc tgctgtgcat gtcgccgccg cctgcgaaaa ggttaggcgt
    55861 gaggacgcct tcgcaagtca gcctcgagcc atcagtgccc cctcttatgg gcgcaactac
    55921 aggatcaaca ttggccatct ccatagcttc caacgctagc ctcacgactc ggggatcttc
    55981 atccaatttt tccttcatat tgcgatagcc cccggtatga gcccacttga tgctgcatcc
    56041 ggggtagttc gcctccagcg cctccaatgt cttgatgaaa gcggtctccc tcttctccag
    56101 aagcttggca tcgaaatcgc ggatgatgac ttttatcgat accttctcca cattgccttc
    56161 gatttcgttg ggatgcgtaa agccttccct gcctgacgtg gtttcaggcc gccagtcggc
    56221 ggggattgca gaaatcagtt cggccgcaat gtggactgca ttcaccatct ttcccctagc
    56281 agttccggta tggctcgaca ctccctctat agttacaaca ccatttattg cgttgaaggt
    56341 ctcctccgaa agctccccga gatatccccc gtctagtgtg taagcgtatt tcgcgcccca
    56401 cttatcatag ggaaatctgg ctaccccctt ccctatctct tcatcaggcg taaaggctat
    56461 ccatacatcg ggtcgaggga tatcagggtt tcggatgagc tcaaccaagg cctgcattat
    56521 accggcgatg ccggccttgt catccgtacc cagcaaggta gatccgtctg aggtgattat
    56581 gtcacagcct attgcagcag caagtgctgg cgaatcctct gggctcaaaa tgacgccatg
    56641 accgatctca atctctccgc ccgcatatcc tctatgcatt acgggtttta ccccttctcc
    56701 cggaattccc ggaacagtgt cgagatgagc catgaaaccg actgccggcg caggcttgtc
    56761 cagatttgac ggcaatctgc ctataacaaa acagtgttcg tccacggcaa catcttcaag
    56821 cccaagtgca cgaagttctt ccgccagcat gcgggcgaga tcccattgtc cttccgtgct
    56881 aggagtcgta tcggtctcat ccgaactttc ggtgttgacc ctgacatatc tcatgaactt
    56941 ctccgcgacg ccttcttgca tcagtgcgtc tttattcatt catcgccgcc ccctcaccag
    57001 acccacggcc taaccagctg ctcggcaatc tcctccgcgc gtctggtgca tgcctcggga
    57061 tccatcgcta tctgcctttt cacaacatcc tcgtaaatta tgaaaccacc cacatgttca
    57121 atcctggact tcatggagcg aagcggtcct tcagcagccc cactgcctga tgtcgcaaaa
    57181 actccaaacc ggcggccgat gccgtcaggc atgttgttga gaaaagcgtt cagctccgca
    57241 gacgtcttgc ccgcgattac gggacccgca atcaaaacca actcatacat gcgcaagctt
    57301 ttcggcatat cctcggctaa gtcggtttcc ctctttcgga acgcagccca acttgaagca
    57361 aaccacccca tcggacgctt ggcctcgagt ttgtgatatt ccacgcggtg gccacgccct
    57421 tccaatgccc gtcctataga cttaatcaat ctctcagttg cacctgttcg tgaatgatat
    57481 acaagcaaag ctctcaccgg cagatcctcc ttctcgcagc atggctctgt ccgcaaacac
    57541 tccttatagc caactttaac cttttaacat tcgcttcagg aatgcctttt cccttctttg
    57601 cggcaaagca tcagcatcag gttcgaaact cgacgacatc gccgtcttca agcacccatt
    57661 cgcgcggagc aaactggccg ccaaaccgcg tagagcccca tactttggca cccttcatgg
    57721 ttcgtggcaa atcgcgatgg atggcttcgg ccaaatcagc caccgtgctc ccggctggca
    57781 gcgttaccgg cgaattccgg tcgggctcct ccttgccggg cggcttggga tagagcctga
    57841 taatcccgag catggcaaat atgatctttg gcaactcgca aatgccatgc tccgaagtgg
    57901 tcgagacgcg aacaagctgt gcttctccac aagcctcagc caagatatct gcattgatgg
    57961 tcgcgtcagg caggtcaatc ttggatgcaa ctattaggaa gcgtttcgca tcttcaatca
    58021 cttttcgttc ccgtaacaac gcaagctgct cgtcgagatc atcgaggcat gaatcagaac
    58081 tggcatccaa aacgatcata accccgtcac ccgtccgcat agaggcagtc aaggcagggg
    58141 gacaattgga agcagagata ggcgggcaat ccaccaactg gatctgtatg ttctcgtatt
    58201 tcatcattcc cgggacaggc agaggtgtgg cataaggata gggcgccacc actactttag
    58261 cattggtaag tgccccaacc agcgccgact tgccggaatt aggcgggcca acaacgaata
    58321 cttgacccgc cccttctctt tgcacacagt agggatcggc cttgcctttt tttcgcaatt
    58381 cccggccttc cgcccgaagc ctagcaagac gtttcctgag atcagcttgc aacttctcag
    58441 tgcctttgtg ctttggaatc gtcctgagca tctcttcgac agcggctatc ttttcatctg
    58501 cggtggttgc gcgcttgtac gcctcctccg cctccatgta ctgcggggtg aggttggcgg
    58561 gcatgcgtgc tcctcctttt ccaggggccc ggctgcacta cgtatgtagg accgttgttg
    58621 atatggcttt cgccaccaca gcagcccctc cttcataggt tcgcagtccg aacagatcag
    58681 ggtcgagcgg ctccactccg gccgaagctc ccccgacaac aaccttccac tcgccctcga
    58741 tcggcagatc tatcctaaca tcttgcctgt tggggttgta aacgacgaca atcttatccc
    58801 actcgtctcc gcacgaagcg ccatccaaca tgaacaccac aacattatct gcaggagccg
    58861 gcaggaatct gagtgaacgc ctgattgcct ctgcagagca cattcggaat gcggggtgtg
    58921 cccgacgaag ctgtatcagc cctctgtggt agtcgaaaac ctccgcataa tccaacttcc
    58981 gcacccaatc gaagcggtta atcgcatcgc ccgcacggta gctattgcag tgtccgcctt
    59041 ttgtcctgag catctcagcg cctccctcca taaatggaat accctgagac gtcattacaa
    59101 tcgcctgcgc caacaagtcc atcttgattc tgtcctcttc ggaatctaac ggcgccgacc
    59161 gggcaatctt gtcccacaat gtgaggttgt catggcacga tacgtagttt atgcactcgc
    59221 ccggctctgt cgcaaaatca gctatgtcgg ccgagtaagg tattccccca actacgcccc
    59281 tctcaattgc acgtccacat ccaccagcgc cagttgcaaa cccacgcctg actccgtcat
    59341 tatcgccttt tatggcattt ctgaatccat cgttgaaaaa acctatcttc aatccacgtt
    59401 gtctgccttt tgtgaacatt ctgtgagatg acagcccgga aagcccaccg gcccagggtt
    59461 ccccgtaaag caacagcgac ggctcgattg catgcaacct caactcaatc tccctggctg
    59521 tctcgtaatc catcagcgcc atgagatcga acctgaagcc gtctacatgg tactcttcga
    59581 cccagtatcg caaagaatcg aggatcaact ttcgaaccat cggcctctcc gttgccaact
    59641 cgttcccgca tcctgatcca tttgtgtacc tgcccgcgca gtcagcgcgg tgataataag
    59701 acggcacaat catatggagc ggcgagtctt ccacagacca cgtgtgattg tacacgacat
    59761 ccataatcac acgtaggccc gccgaatgca tagcttgaat catcttcttg acttctatga
    59821 ttctggcaat tccgcactcg ggcgcaatag agtacgaccc ctcgggaaca aagaaatggt
    59881 acggatcata gccccaatta tagccatcct tgtccagttc gtccacggaa gcaaaatcct
    59941 gtatgggcat cagctggaca tgggtgactc ccagctccgc taggtgatcg aggccggtgc
    60001 aaactccggc aggacctcta gaacccctat cagccatgcc caagtatttt ccgggataac
    60061 gtacccccga atcaggagaa ctcgtgaaat cccgtacgtg cacttcgtat atgatcgcgt
    60121 ctgtcgctgc cccaaagggc ggccgtacat cattctccca gccttctggg tcagtcatat
    60181 ccagatccac aacgacgccg cgaaccccgt ttgcagtcaa tgcacgagca tatggatcga
    60241 cggcctcgtt gaccactccc cctaggtcga cgcggtacgt atagcaaact ccctcaaggt
    60301 cgccggggac agtggcccgc caagtcccgt cacagtccgg cgccatctcg atcgcatcag
    60361 cgacagtcgc ctcggtcgac gagtaaagag ccaccgacac gccgagagct gtaggggcaa
    60421 acacgcggaa cgtcgttgat tctttggaat aaacagcccc gagatcgtcg cctgggtaat
    60481 atagctcgaa cacttcctgg ccgcctacgg cgacgacgga agatgtctcc cttcccatac
    60541 atttcaccat ccacgaaact gcattctagc tgctgcttat ataatacagt atctcgttct
    60601 tcagtgtcca cccaggcatg gcatatttcg ttgctatcct ctaaaatacg tttccatctc
    60661 ttccagagat ttctcgaaca cgtccagcgc tcggtgaata ggatccggcg atgtcatgtc
    60721 cactccagcg tcacgcagaa gatccaaggg atacttcgac cttccacctc tcaaaaactc
    60781 gatgtatcga tccctcgccg ggtctccgtg ctccagtata ctttcggcca atgcaaccgc
    60841 tgccgaaaac cctgtagcat actggtatac atagaaagca ctgtaaaaat gcgggattcg
    60901 cgcccactcg agggcgatcc tgtcatcgag cacgatctcg tctccatagt aatccctgtt
    60961 gagctgcaga tagatgtcgt tgagcgcatc cggggtcagg gcttggcctg cctcatacct
    61021 ctcatgggct atcttctcga actcagcaaa catcgtctgc ctgtaaagtg tgcccctgaa
    61081 ctgctcaagg aggtgattga gcaagtacat tctgcgcact tgatcctccg tcaggctgag
    61141 cagatgttcc ataagcaaca cctcattagc tgtagaggcg atttcggcca ggaatatgga
    61201 gtaatcagag tatacgtagg gctgtgttgt ccaggaataa tgtgaatgca tggcatggcc
    61261 catctcatgg gctaaagtaa acatgttgtc caagttgtct tgccagttca agaggacata
    61321 cggatgggtt ccatactcgc cccaggaata agccccagat gtcttccccc tgctttcgac
    61381 gacatccacc caccccgact cgagacctgc cccaaggatg ctcacgtatt catcgcccag
    61441 cggcgcaagg ccttcacaga cagccttctt tgcttcctcg tatgtgactg tccattcgat
    61501 atccggaact ataggtgcat atatatcata catgtgcaca tcgtcgagct tcagagcctt
    61561 ctttcgcagg gcgacgtacc tatgcatcaa tccgagcctg tccctgaccg cttccaacag
    61621 gttgctgtag accttgacag gtatgttgtt tttgtcgagc gcagcctcga ggcaggtatc
    61681 gtaccggcgc gccctcgcgt tgaacgcgtc gctctttaca gacgcagagt aagtggatcc
    61741 tattgtgttt atcaaagacc cgtacgacga gtacaatgcg tcatacgctt ccttgcgcac
    61801 acgtctgtcc gggctttcca taaaccttgt atatcttccc ttggtcagct ctacggtatt
    61861 tccctcttca tccacaatcg atccaaactt gatatcggcg tcgttgagca tggtgaatat
    61921 ggaggaaaca gcctcgccaa cctccccagc catggcgatc agacgttcct ccggagcaga
    61981 aagcgtatga gctctcatgc gtgatatgtc atgcaagaaa tgccggtaca gctcgagctc
    62041 tgcgttctcc gccatatact cttccaacgt atcttcgtca acctcaagca actcaggcct
    62101 gatgaaagat gcagtggaac tgacctgcac cacaaggctt gctgcccgtt cgtacatagc
    62161 ctgatgcttg gaattcgcgt tatcctcgtc tttccgcatg cgggcatata catagatctt
    62221 cgccgcgatc atattgatct cattgagcaa atcaaggccc ttcagaagat cttcagcgct
    62281 ttgtccgatc ctgccttgca aggaggctag ctccggcagt ctccccagga gactagctac
    62341 atccgcctcc cacaactcat ctgatgcata gatgtcctcc aatttccact tgagttcagc
    62401 aggaacatcc tttcgttccg gaatctccat tgaaccatcc ggcatagaaa cagacattca
    62461 atcatctccc gatttcgaca tactttgaat ccgccagtgc cagagtgccc gttccacccg
    62521 agctcagtaa gaaccgggtt ggcgactgcc tctgctgcct gctgataatg ccctagcccc
    62581 caatgcaggg agatgggggt ggttgcgacc atcgcttaaa gcccaaagcc atcgctatca
    62641 acgcgagatc tctcggccca ctaatggaga gcgccccgcc ccatactgtc gacagtggaa
    62701 taaatcgctt gcagcaggtt gctgatgaac atcgaagtag caaagtgtgc ttccacgggg
    62761 cttcattgta ttctgcatgc accttccatc ctgatcaaga tcttggctcc acacgcggac
    62821 tcttcttgcc ggagaccgcg tcggccagat tgacggatag atcgcccatc ctctcaaggc
    62881 tgttcatcag ctccacaaaa atcacaccag aaccgggaac acatttgccc tcattgagac
    62941 ggcctatatg gttggccctg agcatgttcg tggtctcgtc cagcttctta tggatattgt
    63001 aaacatcttc aacggcttcc atatcattgt cgcgcagggc cactacaccc ctggcaagca
    63061 tttgttcagc cagatcgaat acgccctcca gttccttcat cgcagtttcg gagaatgtca
    63121 gcccctggtc gaatttctcg cgtgcgtaga acatgagttg ctccgcaaca tcaccaatcc
    63181 gctcaacgtc gccgaccgca tgcatcaaac ctgccaatcg acgagattcc gcctcgttaa
    63241 gggatgactg ggccaccatg gataggtata gcacgacctc tctgttgagg tcgtcaatga
    63301 gctgctcgcg ggactcgagg tccgaatcga gttgcgatgt atagccctga gtcagggcct
    63361 ttcgggaata gtcgagcatt tcctggacaa tctcgcccat cctaacagtc tccgcaacgg
    63421 caagatccag cgcaatcgga ggcgtaccaa gcatcctgcg gtcgagatac ttcggtccga
    63481 tctccactac cgtgtcctct ccgggtacaa gccatgaaac cacccgctcc aagaaaccgg
    63541 tcgcgggaag ccaaataact gtgtttgcaa cgttgaacag ggtatgagca ttggcgatct
    63601 gccgctgaat atcacctgac cgggtgaccg cctctacaac ccacttaaac gggcctatga
    63661 atggcaggaa tatgagcgct ccgaagacat tgaacatcaa gtgagccatg gcggtgcgcc
    63721 tggcagtcac actggcgcct atccccgaaa gaatcgcagt tatgcaggtg cctatattgt
    63781 cgcccaaaag aatggggatt gccacctcaa gagtaacaag gtctggcgat gagatggcca
    63841 acgccatcag catacctatg gtagccgcac tactttgcac cactaaagta gtcacaagcc
    63901 ctatcaggac acctaagacc ggattcgttc caaaggtttt catcgcctta ccaaaagccg
    63961 gactatcttg gagtggtata acagacgtcg acatcagtga aagcccgaga aacaagatcc
    64021 caaagcccaa cactatttgg cctacacttt tccagacctt gcgacgagcc accatatgga
    64081 tcaagaaacc gatgccgatt gcgggtaaag cgtaatcagc caagttaaac gcgattagct
    64141 gtgctgtgat agtcgttcct atattcgcac ccataatgac tccaagcgcc tgccgcaacg
    64201 tcatcaaccc tgcattcacg aagctcacaa gcatgacagt agtcgcgctg ctgctttgca
    64261 tgacggccgt cacaagcata ccaaccagga ctcccttcaa gggagtagag gttaaaacct
    64321 ccaatatgtt gcgcattctg tcaccagcag cgcgcctgag gccatctcca gccagattca
    64381 taccgtacag gaacaagccc aaaccgccta taatgccaaa tatcatgctg cgttccattt
    64441 catcactcct gaacgtttga cagaaacaga tctaccaact caggatcgaa ctgagttcct
    64501 gaattccgct taagctcgaa cctggctgct tcgacaggaa gcgcctctct ataaacgcgc
    64561 tcgctcgtca ttgcatcata agcatcacat atcgccagga tgcgccccca aagcgatatt
    64621 tcctcgcctt tcttgccata gggatatcca gcgccatcat atctctcgtg atgctcaaga
    64681 atagctctgt aatcgcgatc actgtatatc ctttccatgc ctcgaatcag ctcagccccc
    64741 cattctgggt gcctctttac cgcgtcatat tcctcggcag taagccggcc gggtttcgag
    64801 agtatttcat cggggaggtt aaccttgccc acatcgtgaa gcctggcgat atactctatt
    64861 ctgtccacat ctgctccggg aagtctacat gcctctgcaa gctgacgggc taatatcgcc
    64921 acacgttcag aatggcctcc ggtatagtga tctttggaat cgatgattct tgccagggcc
    64981 aagttgatct gttcagcatt ttccttatct acaagggctc gctgtgtgta gttcttgttc
    65041 gagataaaaa agagttgagc taaggtaaat agaataaggc ccccatactc gtacatggcc
    65101 attgccatca atcccatgat cacagacggc accagcgagc gcgatgagcc gatgatcacg
    65161 gcaggatcga tcttcacctc gaacttgggc tccgagagca ggatctggaa gttgattatg
    65221 actcctgaca acatcaccca tgacagacca gcaattgcaa acgctgccag gaatctgaca
    65281 tctccaaaat ccacagtggc tgcgggccaa agcgaatgga ataccaatcc ggacaatagg
    65341 gcacacagca tgatgcttgc ccggttcccc aaaaccttga ggatgaaaag atacggcgaa
    65401 ttgttttccc tcaagatccg gatctcctgg atgctcacac tcccaatggc tgcaataact
    65461 gcagtggccc cagggttgta tttgacaaca ggcgtcatca ttatgggaag gagcatcaag
    65521 accacggtgt ttctcaggtt tatgacagac agcgatgatg cccaaagcaa tccgataaac
    65581 gccagcgctg atccgagcgg catatcccat tcaataaagc gtcctactag cacaactgag
    65641 agagctacaa gcgaccacac ataaacatca tgcaaaacgt gcctgtcccg aggcaatcct
    65701 atcactcccg ccatgtcccc gatgctttga tcgcaatcac accatcccca tcaaacaaaa
    65761 ggcccgccgc ggcaagccgc tattggcggc gcttggcgaa gttttaacgg cgcctatctt
    65821 gttacttcat cagaaatgtg tcagcaaaag ccatgactga catcagcagc gtcatggctg
    65881 tagctatcaa tgccgacaga gcaaatttct tcactcgctt caccacccat cggtcgtaat
    65941 taacctggga ggctcagctc atctctcact ctcatctcgc gccgcgacgg gtaattcttc
    66001 tgctcagtct actaggagct caaattggcg gtttaaagcc tgaagaatag atctggcaac
    66061 cgccgtggaa atgtcatccc tcttgtaggc agcccccaaa tacctttggc gttgcccgga
    66121 gctgctatct atgagagtaa tctcggaaat cacccttcca tctgctatct cacaatcggc
    66181 ctgcaacgca tatttttcgc ccaacaactt ttcaatggac ttcaacgtgg cgcgcttgac
    66241 tatattactc agcggttctt caatcgttct cccattcgcc gaacctgagt aacactctcc
    66301 gccaaactct atgtccacat aggcaactac tgcgcctccc tgcccatatt cgacggagat
    66361 cctgctgaga acgggcctgc cgttggaacc agcgtggcca tccggatgtt tctccctgat
    66421 ctgtgctatg ctgattctac gataatcaat gctctcacca gtttccagca agtagacggt
    66481 ctcaatatcc ttcttgagcc ttttgatctc atcctggtca taacagccct caaccattac
    66541 atggatctca tctccatccg ccaactcaag aatccgcact cgggtgactc caggcaatag
    66601 gcgcatgcgc ccttctgctt tagccaatcg cgtctttctg tcatcagaca cccgaatacc
    66661 ccccaagaac atcggatcat tgccgccgtt gcaacactcc gataacttcg gatttgaatt
    66721 cgactccgca agccaaatcc cttcaattcc gaaacaacca tgaaatataa ttgaggtgtc
    66781 gccccgggcg gtaaagacta gcatttcagc tgcctctgtg agcaccaagg ccctattaga
    66841 ccgaatcgcc cctttaccga aataccgacc gacttgtatc gtgagaacac tgccgggctt
    66901 tacattcccg cagcgttggg ctggacgata acggcctctt gccattttcc cgcttggcag
    66961 gtatacttct atccgcagca aggggctggg ttgtcgtcgc atgaagtgta ggccagggcg
    67021 tatgagacgc ccattgtctt cgcaacgttc caatcccact cacctcttct ttggcatgga
    67081 cgatgtatac agtcctcaac ggacccctac ggcacattgg caacaagtac ttgccaggca
    67141 ggcctagcga cgatagtcat ggcatgttct gtcgttcccg acggcccgcc gcccaaatcc
    67201 gtcgtagcta ggccacagat aactctcaac ctcagcctat ctcggcattg cctgttcatt
    67261 cttggcataa gtgctttgcc tgtttgcgct ccgacgacta ttcgttcctg tcctcggcgt
    67321 aatcggcact gcaataatct taggtgaaca gtcttcaagt agaatgaact caacggagga
    67381 tgagcgccca gtgtacaaac tcatagcttg cgacatagac ggaacgctcc ttcgaagcga
    67441 tggcagcacc agtccaagaa cgatcaaggc catccgcctt gcccaggcgt gcggggcaaa
    67501 ggtatcgata gccactggca gaaggataat atccgccatg ccagtaatcg acgcgttcag
    67561 aatggtgcat cctgccgtgg tccactgcgg agctgttgtg ttacaacccg gctccgagag
    67621 tattctaatg gcacagcaga ttccacgcga tacggcgctg cacgtgtgtc atatagcaga
    67681 aaattgcgga gttgatgtca ctgtctttga aagcgtgtat aagggacggg cgctttacgt
    67741 aacgccattt gcgatctctg actgcgccca gccgctctgg ccccgcacgc tgccagaatg
    67801 ccgtgaggtt gccacctatg acgaggcttg cacatcagat cctgtccagt tgtgcatcca
    67861 gggcaacatc gacgccgtgg acaggtttct ggacaagata gatgctgatt tttcgaatca
    67921 ggtctcgttt ttcgactatg gggttttgga ggacggcttt cgcgtagtgg atgtgttcgc
    67981 cgccggggta cacaaatgga tgggggttag cttcttggct gatatgtggc acatcaggcc
    68041 gtccgaaaca atagctttcg gggattctct gaacgatgaa gaactaatag cgcatgcagg
    68101 ccttggggtt gctatgggca attctccgga cgaattgaag gagcttgcag accttgtcgc
    68161 ccccacaaat gacgaagacg gtgtagcggt agtcattgaa tcactggcgc gcagaggtct
    68221 gctcggtcac gaagacagca agagctgcgg agagccgagt gaggagggta cgctttgatg
    68281 tgtgcatcat caattcgaca ctagtcgaca atagcatgga ccggctcata taggtcttca
    68341 gaaacgagtt cgcgcacgcg cccccctccg ggcgtgggca tttcggccca taccctcacc
    68401 cggtatccct caaccccacg tcttatcttg tctgacgctc ctgggcgcgg cggggctaca
    68461 gatcttcctg tagcatccgg aggcgacatc ggcctcgagg gtattgtctc aacaatctcg
    68521 gtaaacagcc ttatatctgg cgctggccct tcgccctcca gccagacctc caagcggtcc
    68581 ccacttgcct cggctctaat tgtcactggc gaaccgctgg tgttgaccca tttcaaatcc
    68641 cgcgcaccat acgacaccgt cgcatcaagc cctgcaggaa gatacggcac aggccgcgaa
    68701 tgagcatgtc gctcggttac ctccatgccc gacagcaaca cggcattata cagcgtagat
    68761 gatagctggc atatgcctcc tgcaatgcct gactcattct tgccatctac aataaccctg
    68821 gcaggtttgt acccctgttc gacgccgcgc tcccctatgg acgcgttgaa ggaaaattcc
    68881 gttccggcat caataaccgt tccatcgagc ttccttgcgc caagggccac gttggcgttt
    68941 ctctccttct catcggcgtt gagcagcgtg atacagcgtc ccagcacgtg cccggttgct
    69001 ttattgacag gcgtggtctc agatggcgca cggcccccgg atgcacagag aagaacagat
    69061 gcagctatga cacctacagc tatagatacg gccacccact gacgcctcat tccccatcag
    69121 ccacctcgat caccctgctc gccccgttcg ctcttaatcc aggatagtac attgaccagg
    69181 ctcgagtagg catcaccatg tagcgtccca gtcgctcagg tctgagaata tagtcaaaca
    69241 ccatgcgttc gcccgctttg atccatgttg caaagaaaac cattctatcg tccctaactt
    69301 ctcgcctatc ataccagtaa ttccaagacc atggatcagt aaagccttcg gatatctcga
    69361 acccagacgg aacaggatcc tcgaaaatca tgtaatccat gtcgcgcttg gcatcaatgg
    69421 cgaccctcac aaggagctcc tgtccgcaca tgacaggagt atcctcatcc acaggaatca
    69481 gcgcatattc ttcgccctcc ggggcctcgg catttctgtc tatgacaaaa tatgtgcgga
    69541 caatgtctgc gcactctcct gccgcctcaa tgcgatcacg ggcttcatgc catgatgcgt
    69601 taatgctgta gtaaacagga cctccctcgg ccgatatctt gaccgagttt ctcccgacta
    69661 ttagaaattc ggggtcgatc ttgacacaca agtttgcggc ctcgtagcct gaatctcctc
    69721 ccaagtattc cttggcaata cgccgcccgt tgacctccac cgtcacttgc cttcgctgtc
    69781 tgacatcctc ctccatagcg gtcatgtact ctaccaacgc catgattgct gcagcggact
    69841 cccgcgttga tctccactgt gacccgtctc ttgtcatagc aagatgtcgg gcagcctgcg
    69901 atagaagcgg actggaaggt tcagcctcga gcaaagccat catggcccat gcagtggtct
    69961 cgccagtttc attcctccac caattttcct tatcctcagt ctcccaaaac gcctgccctg
    70021 aaatcacagt cgccatattc ctcagagtct ccgcgcctgc cgatgccatg tcgtcgcgtc
    70081 ctgcagccga cgccgccagt gttacatatg caacagtcaa tggtttcagc gattgcgatt
    70141 cagctgcaac ccgccttcca tgctccacat tggggtacgc cgggagtttc gcggcaagct
    70201 gttcaagggc cttcatgccc tttgcgccca aggcgccttg ctccgccagg acgtaacagg
    70261 caaatgcgcg gttcgtatcg ctcttctcat cccatactgc ttccctcagg taatcggcgg
    70321 ctcttgccat tgcctcttca cttacacaga acccgctccg cctcgccctt tccaagccgt
    70381 agagcacgta tgaagtcatc cataggccac tgtcgtcgtg ctcccaccaa ccgtacccac
    70441 catcccaatg ctgaaacttg tagaggcgcg caagtccgga attgaccatg tcggcaagct
    70501 cctcctcagt ccttggcatg gggatcccca tacaagcaaa agcgcggtgt gccaatatgt
    70561 cggggaggaa agcactcatc gtctgttcaa cacatccata cgggtaattg accaggaatt
    70621 cgagggcaga ctccacaaca ccagcgtacc ctggagagat atccactgat agcgacgtag
    70681 ctccctgcac tatgtcatcg ggcaaatcaa actcgatgtc attctccccg cgccctccgt
    70741 cagggcgcac ctcgccggcc cagacttcat cgcacttctc tccaaacgga agaaccggga
    70801 cagacaacgc gagtttgtct ccaacaaacc aggaccaggc atttgccttg aagaccacat
    70861 cgcccacggt ctccggctct acctcccatt ccaccacagc ttctccactg gcaggcactt
    70921 tgacccactg ccatgagcag cccacgatct tagcgccgct cgcgttgagg ttcacgaaga
    70981 ctatccgctg ccacttggtc tcgttatgaa taacccccgt tacacgccca cagtcgccca
    71041 tggtatagtg gcgcggaatt ccaagtctta ttgaaagcgg tttgctcacg gtcacctttt
    71101 cctgggcctg cccaacaatg gtggaacgcg tatgtgccct taccgtagct cgccacgtgg
    71161 taagcgaatc aggcatctta acccgaacca ctgcatgacc attgctgtct gtcacaacag
    71221 acggaaacca tgcggctgta tcagggaagt acttgcgtgt cgcctctgtg ccgccttctt
    71281 tctcggcgcc gccataataa gtcaccggaa aagagttctc cgttgagaca gtccgttcgc
    71341 gacgtccgtg gaaaaacttg tcgattgcag gcgtggtgtc gggcctgacc gcgtagatcg
    71401 attcgtccac caaaccgaag gatatttcag ctggaacacc atctccatca gaattctcag
    71461 tcacgatcag atacgttgca gtctccccgg gacggtacga gtctttgttc ggctttatct
    71521 cgacattgag tagacgttct ggaacaggga tcacgatctg gcgcgccgct gtctgcataa
    71581 cacctgacac tattgcaaca acactcacat acgtatttgg ggcatattcc ggcttaatcg
    71641 ccagttccac ctctgctgac ccgttctcga gcctaatgac gcgcacatcc cgaacttctc
    71701 tgtcctcaac tgtaagaagg acagagctta cctcaggcgg cgcagtaatc agaaccttcg
    71761 caacatcgcc tacgtcgtat ctgcctcggt cgcacacaat acccaggtca gtccggaaca
    71821 tgtcggccca cgatccattg tccggagtcg tccaaatata ggtctccgcg attatggcat
    71881 tgccacgttc atcctcggac ttggcctgaa ttacgtaatc tccgggtttt ccggcttcca
    71941 gctcaatgaa gccatcgccc gatgcagcag ttgtgatttg ggttgagtgg acaggcgtgt
    72001 cagtgtagcc tttcttgctg taactccgtt tgagcacgat aatatcgatg tccctgacca
    72061 ctccgttgcc gtcgagatcg accgctttcg cgtatacttc aaatttgtct ccctcccgga
    72121 ctacgtaacg gtcagtcgaa acgtcgacgt caattgtgcc tctggcaaca tcggtgtatc
    72181 ctcttccata tgccctcttg tttgttgcgt caatgacgtc tgcttccact atatatctgt
    72241 aattatggcc atcggctgca ggtgactcga aggaaatccg cgctaagcca tctgagtcgg
    72301 tccgagtttc gcctgaaaga acaaaatccc cataatacaa cccaaccatg tcatctacgc
    72361 cgccatactg gtcataatcg tagcccgaat cttccgcagt tgggccgaaa aagcccatgg
    72421 gctggcgata gacacgatag gccacccggg cattccttac aggagctcca aaatagtaaa
    72481 gagctttgca cgtggcatcg agacgatcgc cggcaatgta catctctttg tcgaactcga
    72541 tctccacaac ccattcaggt tttcgatact ccgctacctg aaagctccct tcatgctgct
    72601 ctccgtcaac aattgcacat atccagtaat ggcctaatgg cggttcagct ccgagcttga
    72661 actcgcccga aaaagaaccc caatcgttgg tgagcaaatc ttgcttatag acgacattgt
    72721 tgcgagggtc gcgcacttct accctgacag gtcgacgagc cagtacttca taacccgaat
    72781 tggtaatgcc ccgggcgatt cccttgaaat aaacaacgtt gttcggccga taaacaggtc
    72841 tatcagtata cacataaacc ttttgcgtcc gtgcatccca ccagtaagac gagttgagca
    72901 cggcgaagtc atcgcccttt gctgccacta caaaggccga atctgcagaa tcggccaggt
    72961 ctatagtgaa tagccctcga ttgtctgtca tcccgtaaac ttcgcctgga acagaaacgg
    73021 agccgacact caccacagca gccccgccga tatcgccctt ttctccagac tccaccgtct
    73081 cttggtcaaa acgctcagac gcatcagaac cgctcatgct cgagagttta gcatataccg
    73141 acacgttggg catgggttcg cctgttctga ggttgacggc atagattaca agcccccgcg
    73201 agtactgctt tgagatcagc cccaactcgc tcacggcaaa ccaagccgct tcctccgtct
    73261 tgagctgccc ggatctggca actgccacgt attgtcctgg aggcagaggt ttaagtttga
    73321 cggctgcatc aagcgtgccg cctggcctcg ctatgtcgac acggacagag cgcacctttt
    73381 cccgccccgg cagatccttg tagttcacat tccacagagc actccaatcg ccattcttga
    73441 agtgccggaa gccatcaaat ttgtaaatat caagccaaag ttcctttaca ccgtagcccc
    73501 tgattccgag ttcaggttcg ttttcgggca agtaattcct tgccgttgca aaaagccaca
    73561 cctgcggttc ccgcgcgctc atcgccgctg gagccataaa caaggcaaga agcgcggcca
    73621 aaacaattgc atggctaacg cgggctgatc ttcctagaca acgcataatc attacctcca
    73681 agccctatcg gccacgtccc aaatccaatg agcaaccgaa cttgaccgtc tggaatctat
    73741 aaatctcgca ttcttcgtcg ggctctatgc ctgcctcatc cctcgcccaa ctcaattgct
    73801 tagagtgtgt caaggcctct ccaggcaaaa tcacgccaga cctgtgacct gctctaacaa
    73861 aaaccccaaa agcacgcgga tcccagtttg cgtcggggga aaccctctca agtcgaccca
    73921 caacagagac tgcatattcg caagtcggaa gctcccaggg ttctatggga ggacgcctga
    73981 gatcgcgagc tgcaacctcc ctagctgact gcgctatctc ctccgccaga ttggcgcatc
    74041 ttggccatat cgatccgacg caggccctaa cagagttgtt cctgacaacg gtaacaaaaa
    74101 cccctgcaga acgacgcgtt aaagcctgtt ccacctctgg aatcgcgctg accgacatga
    74161 tcagctcggt cgctgatgga acaggctgcc cccgaacgga gagggccaca cactcccgcg
    74221 caatggcaat cacagggcct tccacgtttg agacacctac catgttaagc tccgacagta
    74281 catcccaagc tcctgccgcc ggcatgtcta cagcagatat aaagtccgcg gcaccttggg
    74341 aggcgggcat ctcgttgtcg ccgaccacgc ctgcataggc caatatggca cacgcaatgc
    74401 atgccaccat caatgcgaac atatgttgtt tgcgcgaaag gaaaagcctc attcgaagac
    74461 accctttaag aggagagcat gaagagcatt gcctggatgg gacagtgccg cgcccctctc
    74521 taagcgtatt cgacagatga gagcatcagt cctactcaag agagggggcg gcgataagac
    74581 acttactccc gaatatggct tggctcaacc acacttgtca tttctgcagc attgggttga
    74641 gcgattcgct gcgggccttc cggctgaaag acatcggcat aaccgtgacc aatgggatca
    74701 tcctcgtgga cagactccgg atccacatgt accatgaccc ctattacgtg tggacacacg
    74761 cctttgatgg ccttctcagc tgccactgct atgttgtgtc cctccaccac cgacaaagaa
    74821 ggatcaactc ctatgcgcaa gtctacgtgc gcatagcctc catagcgcct cgcggccatg
    74881 taatgcacgt cgcttacgcc ttccacagaa tcgatagcct cctgcacagc acggagcgtg
    74941 tccggatcag cctgcgcatc catgagctcg tcagaagact tcctcgtgat ctcaaagccc
    75001 atcttgacta tgagggcggc taccactatc ccggctatgg gatcgagcac aggcactcca
    75061 agtcgggcgc cgaggatgcc tatgaatgtt ccaactgagg aatatgcatc agagcggtga
    75121 tgcagtgctt ccgccatgac tgcgctgctc tctattgcct ctgcagtggc agccgtgatg
    75181 cgatacatgg cttcctttac aactattgac accacggcgg ccacaagggc aatcttgccc
    75241 ggttctgtat acccgcccac cgcgatcttc ctagccgaat tcgatccgat ggcacaccct
    75301 gcaatgatca ggatcaaacc aacaatcttg gcggctaccg gttcagcctt gccgtgaccg
    75361 tagggatgat ccacatcggc aggccttttc gatactctga ggctcataat gacgacagcc
    75421 gtagccaaca gatcagaagc tgagtgggcg gcgtcagcca ccatagccga gcttttgccc
    75481 acaatacccg caattagctt gaacgccacg aggcccaagt tggcaatcat attgccccat
    75541 gacaccctcg cccctatctt gaacctgtct tccgaagaat tctctgttct cacaataaca
    75601 ccccttgcaa gcagtttccc cgaccgaagc gaaacggcac ccgcgggact ctacgatgat
    75661 ctcagtccca cgagcgccgc ggaatgcact cgctgcctag aggcccggcc ccacccccca
    75721 ttccacctag ggggatcgcc tgctctgagt gaaccatgtc aaaaaccttc aggtatgcag
    75781 tctctagtcg gattcatttt gatttaatcc taacgcacat acattgctca gtcaacactg
    75841 ccccatattc cccggcagct tttccaatgc tctactgttt cctgtcattc gcccactgcc
    75901 gccacgttga tctgaaacgg catggctttg attcatacat cgactacgca gattgccatc
    75961 atgcaaatcg ggggtacccg aacaatctcc actgattatt ggttcaggct gtactcgcat
    76021 ttgtctcgta caagcgtcat ataagagata tagtataaaa tagagcataa tgggatattc
    76081 tcggtcccac gctgggagga tgacttagtt gaaaacgaat cgcccgacat cagttgcagc
    76141 tccttcaaac ggaaagacgg tctcctttgc tgttctcacc gtcgcctgca tattcatatt
    76201 cttcggcagt gcaacagcgg gagccgaatc cggattgcat gcaccttatc tcataatcca
    76261 cgtcgatgcc gtaccgtcta gactgttttt cgaagaatac gatgcgggcc atttgccgaa
    76321 catcaaggca gttttcgacg acggcaaatt ggtccgctat gcggtctcgc cctttttcgc
    76381 aggcacagaa atgctttatc caaggcttaa aaccggagca ggcccagatc aaccccttcc
    76441 aatcacgtgg gaaatgtacg accgcaactc gggacggcac atatcgcgca ttgaaaccca
    76501 agtaatactc ttttcccact tgcccacata ctcgctcacg cagatcatcc atggcatccc
    76561 cggaatggac tatttgtctg gctgggctct gcttaacact cctcggctgc tcgatgagta
    76621 tgacatcgtc gagttctttt ggttcataac cgactcttat ggccatgtct tcggtgaaga
    76681 agcccaaaga gaaagtctgc tgatactcga cgagtttcta ggaattctaa tgaagcagct
    76741 gggttcgaag cctgttaacc tggttatcta tgctgaccac ggcatgtcga tgttcgaacg
    76801 gacatatgat gccgaaagcg ccatggctga aactgcgggt gacaatgagc aattcgtgta
    76861 ctaccctaac ctgtatctga aggacagaac acttgccgcg actacggcag agcaactcgc
    76921 caatcgggac gaaatcgact ttgccttcta ccgcgcagat gagttcctta taataggcat
    76981 tcacaaagag ggccaggtga tgttcagatc tgaaggcaac ctgataacgt attcctgcga
    77041 aggaccagat cctttcggat acgccgattt aggctgtttg gggcggcctc tgtccgatca
    77101 agattggctg gaaatgacat gcacctcgcg ctacccagcc gtgccagttc agatagtcag
    77161 atacatggac aatgcccttt ccggcgacat cgtagccgtt ttgaatccac cgaaggggct
    77221 caggaccacg gtgggatttt gcggatgtca tacagggttt gtagataccg attgcgtagt
    77281 tggcgttctg atgaaaggcc ccgatattga cccttcgcaa attcccgaca ccttctggct
    77341 gtatgaaatg tacagccaag tattgaagat agatccacag aacccgacaa aggccgacag
    77401 agctaaaaac ttcttcatgg tttcagatgg cggaattgat ctgcaactct ccgcgttccg
    77461 aaataacacc gcgttgtcaa taggtccaca ggaatggacc tttatcggcg agacaaccat
    77521 tgcgcgatcc tttatcacac aagtgcgggc aggactcggc cttgcttctg ataaaagcct
    77581 tgccggaggg ctaacaccgg tcgcctcctt gaggctagac atctactttg gcccgctcac
    77641 ctacgaccac agaaacacaa tagccttgac ggatggatgc cggaagtcaa cttcgacact
    77701 gcggtttgag gcaaaaaacg gcgtggttct cgaatgggaa taccccaacc gaatcaagtt
    77761 gggggtttct tggtgatcag cagcacgggt atgctcacca actgctgcct ttgacatcgg
    77821 ggaaatggta ttttggcaca ttcatcgtgc tttcatccca cccccaaccc cgccaggcca
    77881 cacgcatgcg cgtggcctgg catattcctt cccacgttcc gcatgaagca gatggctgcc
    77941 aatagcccca agccctatgc tgcccaagca actcgcaaga atatcttggc ccaatcaaac
    78001 tagaagagct tcagctacaa gtaatacaga tccgataatt gcgcagcacc tatccaagct
    78061 tgctgtgtta ggcagttttc gataactcaa tgcgcttgtg ggcacgacag acgcaaggac
    78121 gggtttaccc gtgtcctgca caagctcgta gagaccgttc tgttctgcag gaaacgacac
    78181 tatgacagaa tccacatatg gaatggctct gccatataat ctaaatcgta tgggtaagct
    78241 ttattaacac actcgatata actttcttat atttttgtga caagtcgctt gacaccgatg
    78301 ctgtagttca tctataatgt aacaggaacc caaagtatcc gaggtgaccg aagttgaggc
    78361 tacacggccc tgatgaacca gtctacacca ttggcgtagc tgctcgtttg ctaggagtaa
    78421 gcccgcaaac cctaaggatg atcgagcgtg aagggttgct tgaacctgcc cgaaccgaag
    78481 caaacacgag gctttactcc gaaaacgacc tgctgctctt gcagcgaatc tgtttcttgg
    78541 ttcgggaaga gggcgtcaac ctcgcggggg tcaaaatgat cctcgagatt gaatcaaaac
    78601 aagggaacac aacaatcagg tatcgcgagg ttacgcagag cgttccccgc gaagccaagc
    78661 ctggagaagg caacggcaac tcctgagtta gggtttccat tgttccagtt ttctagttga
    78721 gcaacggaag gggagaatca caatggggaa agttgttggc attgacctcg gcacaacaaa
    78781 ctcagtaatt gctgtgatgg agggtgggaa ccccacggta atagtgaatt cggaaggagc
    78841 ccgattaacc ccgtcggtaa tcggcttcag caagagcggc gaaaggctcg tcggtcaact
    78901 tgcacgccga caagctgtta tgaatcccga aaacactgtg tattcaatca agagatttat
    78961 gggacgtcgc ttcgacgagg tcgaggagga acgcaatcgc gtaccttacc acgttaaagc
    79021 ctcctcaaat tacggcatca aaatcgacgt gccggcaact ggcagggatt actctcctga
    79081 agaaatctct gcgatgttac ttcaaaagct gaagacggat gcagagaaat tcctgggcga
    79141 accgattacc aaggcggtaa ttacagtccc tgcctacttc aatgattcac agaggcaggc
    79201 aaccaaagac gcgggacgca tcgccgggct cgaagtgctg aggatcataa acgagccaac
    79261 agccgcagcg cttgcatatg ggctcgacaa gaagaaaaac gagacgatcc tcgtctggga
    79321 cctcggcgga ggcacattcg acgtctccat tcttgaggtg ggcgaagggg tattcgaggt
    79381 aaagtctacc gcaggcgata ctcacctggg cggagacgac tacgaccaaa gattagttga
    79441 atacattgcc gacgaattcc agcgcgacca gggcatcgat ctgagaaagg atcgtcaggc
    79501 ccttcaaagg ctgatagaag cggcggagaa ggcgaagatc gagctttcac aggtttatga
    79561 gaccaatatc agtcttccgt tcatcacggc agaccagacg ggtccaaaac acctggaaac
    79621 gaagatcact cgcgcaaagt tcgaagagct cacatcggac cttacagaaa gatgcactag
    79681 gccgttcaag caagcgctaa gtgatgcaaa gctctcctta gacgacatcg atgaagtgat
    79741 tctcgtgggt ggcgccacgc gtatgccagt cattcaggaa ctggtcaaga ggcttacagg
    79801 caaggagcct caccgaggag tgaacccaga cgaagtagtg gctgtcggag cggcaatcca
    79861 ggcaggcgtc ttagccggtg aagtcaagga cgtagtcctg ctggacgtca cccccctctc
    79921 ccttgggatc gagacccttg gcggagtctc caccaagctc atcgagagaa acactacgat
    79981 acctacgagg aagagccaaa tctttacgac agcagaggac gggcagacct cggtagagat
    80041 tcatgttctt cagggcgagc gcgaattcgc gcgcgacaac aggacgctgg gtcgatttac
    80101 actgcagggc atcccgccgg cgcctcgcgg agttccgcag atcgaggtgt cattcgatat
    80161 agacgccaac ggaatagtca atgtcgcagc caaggacctt ggaaccggga aagagcaacg
    80221 catcacgatc actgcatcaa cgcagctgtc gaaggatgaa gttgaaaacc tggttcgaga
    80281 agcggaacgg gctgctgcag aggacaagaa acgccgtgag gaagtggagc tcaaaaactc
    80341 cgccgacagt ctcgtctacg tgacagagaa gacgctgcgg gaaatgggcg acaaagtacc
    80401 tgcagaaaca agatccaagg tcgaagcgga acttgaggaa ctgaaaaaag ccatcgccga
    80461 cgacgacatg gagtctgtaa agcgcttcat ggaccaggtc agagaagcct cttacaagat
    80521 gtcagaggaa atatacaagg cagcgcaaga agcctcaggc ggccagggtg aaggtgacta
    80581 cggctctgat tccggggacg ccacaggcgc cggagggttc gattcggcaa atagcgatga
    80641 tgacgacgtg atcgatgcag acttcagaac tgtagacggc gatgacaact gagcgagtga
    80701 gccatcttga actcggacta ggcccctgcg cgggccttcc gaatgagtcg aagccctgcc
    80761 ctaccctatg agggcagacc gagacatagt ttcgagacaa aactacattg ttaaactgga
    80821 ggtgcgtttg atggcgttga tgaattacga tccgtggcag gagtttacgg aaatgagacg
    80881 tgttttcaac cgcatgtttg gagatggatt ccttgggctt cccatggagg agcagagcac
    80941 acacggcagc ctgttaatgc ctgtggacat agctgaaacc ccagaagagt tcgtgatcat
    81001 ggcagagctg cctgggatgg atcctgacga tatcgaaatc acccttacag acaatcagct
    81061 gacgatcaag gggcagcgtg ctgccgaata cgaagacaaa cgcgagaact acctgcgcac
    81121 agagagacgc ttcggaagct tctgcaggag cttcgcactg tcttcacctg ttgctgaaga
    81181 caaagtgtca gctaattaca agaacgggat cctcgaggtg catctgccga aatcggaagg
    81241 cgccaagcca aaacagatcg agattcgtaa tgagtaacag ggctgaactg taggcgtggc
    81301 gtgcggtccg taagcggcga cgacagaccg gaaaacggcg gtcactgctt acggaccgtc
    81361 tcgcaagcct caccaaacag ccacttacca ataacgagcg gtggtgtaga taatgagccc
    81421 agacacgcaa gacatgcgcc tcggcaatat ccaatcgtcc aacagcagcg aggtcactgg
    81481 tgctgaagct aacggagagc acacacccga agatcttccg tccaatgcgg acatggtcaa
    81541 tgagggtgat gaattgagac tgaaactggc tgaagccgaa gcaaaggccg aggaaaactg
    81601 gaatatgtat cttcgcgcct tggcagatct cgaaaactac caacgcagga cggcgcggga
    81661 cctagaattc accatcagga gcggcaagaa ggagatgttc ctcaagctcc tggcaatcgt
    81721 cgatgacatg gagcgggcac tagcggcaga cgctgactac gagtcccttc atcaaggcgt
    81781 tgagatgatt atgagaaaac tcctcgacgc actggcatct gaaggggtca aacctataga
    81841 cgctctgaac gaaccattcg atccccgcct ccatgaagcc gtggccgcat gcgacggtga
    81901 ggatgtcgaa accgagactg tagtcgaaga gttcaggcgc gggtacacat actgcaacga
    81961 atgtttgagg ccgaccatgg cccgcgtagc agtaccgaaa tcggactagg ctgcgcacgg
    82021 ccaatggcat gggcgcgccg gggagatgga ctatggaatt caaagattac tataaagtcc
    82081 ttggcgtatc caaggacgcg accgaagagg aaataaagtc cgcctatagg cgacttgcgc
    82141 gccagtacca ccctgacgtc aatcctggtg acagttcggc agaggaacgc ttcaaggcga
    82201 tagccgaagc ctatgaggtg ctgggcgatc ctgaaaagag aaagcgctac gatatgctcg
    82261 gcgccgactg ggatcattac tcaaaaacca gcgctggctg gcctccaggg ggacgacgaa
    82321 cccaacctgg gtacgggcgc acaacgttca cctttggcaa tctttcggac gctgatttta
    82381 gcgatttctt taagcaattc tttggagatt tgggacttgg agaccaccta ttcggacatg
    82441 atctgcacga tctcgacgaa cgcagtttca gccgacgcag gccggtcaag cgtgatatcg
    82501 agcatgaact cgaaatcggt cttgatgaag cattcaccgg cagccaacgt tcgatcgaaa
    82561 tcgtcgaccc aaccgacggg tctcggcgga aaatagtagt cgatattcct ccgggggtgc
    82621 gcgatggcac acgccttcga attgccggcg aaggagcagg agctccggga ggccgtggca
    82681 atctctatct taaggtgcgg atacgccccc atcgcgggta ccagctccgt ggcgacgatc
    82741 tttatgtgaa ggtgaacgtt cccctcactg tcgcggttct cggaggcgaa gtccaactgc
    82801 agactgtgac aggggacaca atttcggtaa gagtgcctcc ggagacccag aacggacaag
    82861 tgttgcgatt gcgcggactt ggcatgccgt caaccaaggg aggaaaacgt ggcgatttga
    82921 tggtcaaggt aaatgttgtc ttgcccagta acctaacaga acaagagcgc gaactcttcc
    82981 gagaacttga acgactaacg agcgccagct cgtcagggag ggatgaggca tgagatttga
    83041 agaacttaca gtaaaatctc aggaggcaat cgcgcgcgcg cagagcatcg ctcaggcaaa
    83101 ccagcatcaa agcgttgcgc cgatgcacat tctagcggcc cttcttgaac aggaggaagg
    83161 cgtagtccct cttatcctat caaagcttgg cgtttctgcc gaggtattga caaggaccgt
    83221 tgcttcatcc ttagagaagc ttccaaaggt ctatggccaa gaagccggtc agtacttgtc
    83281 tacagccacg gccagtctgc tcgatgatgc ctcatctgaa gccgggcggc ttaaggatga
    83341 gtacgtaagt accgagcata tcctcctggc cttggccgga gctgatgatg attcagggcg
    83401 cctgctcaga tcgatgggag tgaccaagga cgccatatac aaggcactcg tcgatgtgcg
    83461 aggcacgcat cgggtgaccg accagaaccc cgaagacaag tatcaagcac tggaaagata
    83521 cacccgagac cttactgacc tggcgcgcaa aggaactcta gatcccgtca taggtcggga
    83581 cgaagaagtc cgccgggtga tccaggtcct ctctaggcga acaaagaata atcccgttct
    83641 catcggagag ccgggcgttg gcaagactgc catcgtggaa ggtctcgcgc aacgcatagc
    83701 cgccggagat gtgcctgaca gtctcaagaa caagcgggtg ctctcgttag atctaggtgc
    83761 actagttgcc ggaagcaagt atagaggcga attcgaagac cggctcaaag cagtgctgaa
    83821 agacatagag gatgccgaag gacagataat tctgtttatt gatgaactcc acacactcgt
    83881 aggtgctgga gctgccgagg gctcaatcga tgcctcgaac atgcttaagc ccgcactagc
    83941 ccgaggcgaa ctgagagcag ttggcgcaac aactatcgat gagtatcgta agtatatcga
    84001 gaaagatgcc gcgctcgaac gcaggttcca acctgtgatg gtagatgagc cctctgttga
    84061 ggacaccatc gccatactga ggggactgaa ggaaagatac gaagtccatc acggagttaa
    84121 gataaaggat tcggctctcg tggcagcggc cgttttgtcg gatcggtata tatccgaccg
    84181 gtttttgcct gataaggcca tagacctcat ggacgaagct gcgtcaaagt tgagaatgga
    84241 aatcgactcg atgcctgctg aaatcgatga agtggagcgc cgtattgtgc agctggagat
    84301 cgagcgccac gcgctcgaca aagaagatga tgaatcatcc cgagaaaggc tcatcaggct
    84361 caacgaagag ctcaaggagc tcaaagagaa atctgaggcc atgaaagcac actggcaaaa
    84421 ggaaaaagag actattgcca ggatcagaga gataaagcag aaaatcgaag aggccaagat
    84481 agaggagcag gcggcagaac gtgccggcga tctcggacgc gcagctgagc ttcgctacgg
    84541 tgtccagctg tctctccagc aagaactgga tcgcgcaaac caggaaatcg ccgagctcca
    84601 aaaggagcag cgcttcctca aagaagaagt ggatgatgaa gatatcgctg agatagtcgc
    84661 gaaatggact ggtattcctg taacgcggct actggaggga gagacggaga agcttctcaa
    84721 gatggagcaa ctcctccgga gaagggtgat tggacaggac gaagcgatag aggcggtatc
    84781 aaacgcagtc aggcgtgccc gctccgggct ctccgatccc gagaggccga tagggtcatt
    84841 cctattcctg ggccctaccg gtgtaggcaa gaccgaactg gcaaaggcgt tagctgaatt
    84901 cctcttcgac gatgaacgcg caatggtaag gatcgacatg tcggagtatc aggagagaca
    84961 cacagtggcc aggctgatcg gcgcgccgcc aggatatgtc ggctacgaag agggcggcca
    85021 gttaaccgag gcagtgaggc gacggcccta ttcagtcata ttgctcgacg aaatagagaa
    85081 ggcgcatcag gaggtcttca acgtcctgct acagctcctc gacgatggca ggctgacaga
    85141 tggtcagggg cgaaccgtag atttccgcaa cactgtgata ataatgacct ccaacctggg
    85201 cagccagcac atcatggaac acgccacagg ctctgaggag cagatgcggg atctggtgat
    85261 ggaagtgctt cgacaagcat tcaggcccga gtttctcaac agaatcgatg aaatactgat
    85321 cttcaatagg ttgggacggg aggaaatatc gcagatcgtc cagatccaac tcggcttggt
    85381 ccgcgatcgg gttgcatcac aaggtttctt actcgagtgg acgccccagg tgcgggactt
    85441 cctagccgag acgggatatg accctgtgta cggagccagg cccctcaaac gtgccattca
    85501 aagagaactc gagaatccgc tggccatgga gatattgcaa ggcaagttcg cgcctggtga
    85561 cagaataaag gtagttctca cggacgccgg cgaaaatggc ggagctctgg cgtttcagaa
    85621 agcctaactc aagaacggac atctacgcgg atgctcatcg ccacagatga atagatatca
    85681 gagagagggg caaccctctc tctcgctttc cagggaaagt ccgaccatgc agcaaagaaa
    85741 ggacattgcc ctctctactt tcggtttcat caaaccccca taaagaaacc ggtggcggat
    85801 caggcattcg tggttgagtc aagctctgag agtgctttgt gatgcgtaat ctacagcaca
    85861 ataatagcca catgccaact atgagtcata gcaggcgatt gcctaccaat gtcgaaacat
    85921 tcgtcagaat cactgttggg aggggttcaa gtgactggaa agatcggcaa ataccacgga
    85981 accaccgcag gggcctttct ctatagcctt ggctcgttca gcgcagcact tctcggacaa
    86041 gccttcaatg cttgggttca gctctactac gtcgacattc tgaaaatgcc attagcgctt
    86101 tacggaacag gcatggctct ctatggcata tggaatgcca tcaacgaccc cctggcagga
    86161 caatggtcag acaacacgcg cacgcgctgg gggcgaagga ttccgttcat ccttttcggc
    86221 actattccat tctgcatcat cttcgccctt gtgtggtggg ttccccagtg ggcgcaggca
    86281 agctccaggt cgctgttcgc ctattttctc gtcaccgcct tcctcttcga cgggctattc
    86341 acagtagtcg tcctgaactg gaccgccctc taccccgaaa tgtatccctc gctgacacag
    86401 cgttcccaag tatccgccct tcgccaggcc ctcggaattg taggaatgat cataggcatg
    86461 gtccttgctc ctcagttcag gcaatggttc ggctgggccg gaatgggtat tctcttcgcg
    86521 gtcataggcg gattgagcat gttcctaagc ctgctcgggt cgaaagagaa tccagacttc
    86581 agcgagcagc aagggctacg cgtaagtgag gccttcaaac acaccttcgt gaacaagtcc
    86641 tttgtaacgt tcgttctcgc aagtctatta atccagttca ccttcgttct gttgccagcc
    86701 accatgccgt ttttctgcaa gtatgttctc ggactcaccg acgctcaaaa tacgctcgtg
    86761 ttggcgccca tatttctagt cgcgcttcct atggtttggg tgtggggacg tgtgacgaat
    86821 cgcatagggc cgcgacgcac agcaatggta gcagccgtcg tgtttgcggt agcccttatt
    86881 cctttcggat acgtcacaac cctccttggc gggatgatca cttcggcctt cgcaggcata
    86941 ggcctcgctg gcctcatggt gattatcgac gttctcatag ccgacgtcat tgacgaagac
    87001 gaactcggta ctggaacccg tcgagaaggc gcctactttg gcgcgaacgc atttgttatg
    87061 cgccttggag tctccctcga ggctatcacc ttcagtgcga tcatgggaat gcacgggtac
    87121 aacccgaatc tcgcctctca gcctagcaca gtcattccag gtttcaggat tctcatgaca
    87181 gtagtcccca tagtctcgct agtgctagca ctgatagcac tctattacta ccccatcgac
    87241 ggagaccgta gagaagccat catacaggga atagagaaga agcgcagtgc ggaagggtaa
    87301 tgtgaacatc tctcacagct ttcacagtaa actgtagcat cagcatggcc cagatggagg
    87361 ttggttccat ggatctatcg gggttctttc actacccaaa tctggaagtc ctaagctcca
    87421 tattgtgcgt tcaacctcat ccgcacgaca gtcaaccatc ccacgctaaa ggcccggccc
    87481 tggttgacta atctaagtct taactggtta cgttttgcct aagacctgaa atgcgcgtcc
    87541 aagtcccgga cactgcatct gaaaggatta tcatactggc aaactcaaac tcgatttcaa
    87601 gcgggaccag ttcctcctag gttagccagt cttcgggcct tgcctcgcaa caacaccctt
    87661 gtcattaccc tcgttcggga ctttcatctt attagcaccg aacagcacga tgaggcgcgc
    87721 agacgaatac tcgtctgcac gcccttgctt tgcactcaga caaatactat ctagatccat
    87781 gctcttgcgc gaggggcaac ccaaattctt cgagcttaac aatatgtcct caagccctct
    87841 tgtccaactc ttccctgagg atcctgttag tctcctgagg atttgcccgg ccacgagtct
    87901 ccttcatgac ctgacctacc aagaacccca acgccctctc tttgccggca tgatagtcag
    87961 ctacggggcc aggattagcg tcgatcacct tttcgacaat ggtcacaagt tcaccaacat
    88021 cgcttatctg tagcaatccg cgctctttca ctatgtcgct tgggcgcctg ccgttactaa
    88081 aaacatcctc aaagactgac ttggcaatct tgccggatat cttcccagtc gtcatcagat
    88141 ctatgagctc tgcgaaaagc tccggggtga gtttggattc cgacagctca atacctgtag
    88201 cattaagaga cgccatgacc tctcccaata tccacttggc aacctctcgc ccctctccca
    88261 cgatggcaca ggtcttctcg aataaatccg aaaggtctgc ggagtctgcg atctggcaag
    88321 catcatactt cggcaaatcc cattcgcgca tgtatctctc agccttggcg tcaggaagct
    88381 caggcagctc aactcggatc ttctccaccc atgccctatc aatcacgatt ggaccaaggt
    88441 cgggttcggg gaaatagcga tagtcatgcg cctcttcctt ccccctcatg aacgcagtct
    88501 cgcctttggc atcgtcccaa tggcgagtct ccctcgccac ctctccacca gagctcacaa
    88561 tctctatctg cctcgccgcc tcatactgaa tggccctgtg cacggcccgg aaagaggcga
    88621 tgttcttcaa ttctaccaac gtaccatact gttcagaccc gcgaggcatc accgagatgt
    88681 tagcttcaca cctcatggaa ccctcctcca tcttgcagtc agatactccg atagcaagca
    88741 atatggagcg aagcttagtg aggtaagccc ttgcctcatc agcggacctc atgtccgcct
    88801 cagatacgat ctcgatcagt ggaatccccg acctgttgaa atcgagcagc gaatggctgg
    88861 cggaaataat gtcatctccg gcatgcatca gtttggccgt ctcttcctcg agatgaactc
    88921 ttttgatcct tatccgtttg gactcgccat tgacatccac gtccatgtat ccattgctcc
    88981 ctattgggca gtcatactgg gatatctgat aacctttggg caagtcagga tagaagtaat
    89041 tctttcgatc gaatttagta tagtctgcta cagaacagtt gagggccaaa gcagcgcgaa
    89101 tcgcgaactc caatgccctc ttgttgagca ctggaagagc gccaggcatg cccaagcatg
    89161 tggggcatgt ttgggtattc ggctcggccc cgaaagctgt cctgcaccca cagaacacct
    89221 tggattcagt agccaactcg acgtggactt ctattccaac aacaacttca taatccgaag
    89281 tcatctgtca cgcccccctt tcatggcttc aagtggcggc cgctttaccg actcgccgca
    89341 tgcctgctcc aacagatggg caatgcgaaa tagtgtgccc tcgtcaaaag ccctccctat
    89401 aagttgggct ccgacaggga gtcctttaag ggtcccgcat ggcacggata ccgcaggcat
    89461 gcctgaaagg ttagctatgg ttgtgtaaac atccatcaaa tacagggcca cggggtcatc
    89521 tagcttttcg cctattctga acgccaccgt cggcgacgtc ggcgagatga tgcaatcaca
    89581 tttctcgaga acttctttca cgtcacctag tattagagtt cgtgccttca gtgccttgag
    89641 atagtactca tcataatgct tcgagctcaa cgcgtgggtg cccaacatga tgcggcgctt
    89701 cacctcggga ccaaacccct cgcttctgct ctgccttatc actgcctgaa cgccatgggc
    89761 atcctcggct cgaaagccat acctaatccc atccatacgc gccaggttcg agctggcctc
    89821 ggcggtggca ataaggtagt agactgcaat cgcatagtcc gaatgtggca acgatacctc
    89881 cacaattcgc gctcccgcgt cttccagagc acgcactgca ttatcgatca gttccctgat
    89941 gccactatcg agccctgaat gcatgaattc ggaaggcacc ccaatagtaa aatctctgtc
    90001 tgtctctttc agagatccgc agtaatcagg aaccggttca ggtgaagacg tcgcgtcacg
    90061 ttcgtcacgg cctgctattg ctctcaacat gattgcacag tcccaaacgt cccgggtaat
    90121 agggccaact tggtctagag atgacgaaaa tgccactact ccgtatctgc tcacccgtgc
    90181 atacgtcggc ttcaatccga cgagtccgca gaatgaggcg ggttgccgca ctgaaccccc
    90241 tgtatctgtc cctaaggcgc ctatggcctc tccggccgcc accgccgccg ctgagccccc
    90301 gctagagccc ccgggcaccc tctcaaaatc ccacggattc cgggtaacgt ggaatgccga
    90361 gttctccgtt gatgatccca tggcaaactc gtccatgttg gtcttcccta caattactgc
    90421 gcccaagtcc ccgagacgcc tggctacagt cgcatcgtat ggcggcacaa aatccgcaag
    90481 catcttcgaa gcacacgttg tcctgatgcc ccgggtacag atattgtcct tcaatgccaa
    90541 tggaatcccc atcagcgggc tgatatcaga gagacttcct cttttccgcg cttgaagcag
    90601 tcgcttatct gccgcagctg cctgttcaaa cgcctggtcg cgggcggtgg tcacataagc
    90661 gccaactgat tcctctacct gatctatccg atcaaagaca gactcggtta tctccgtgga
    90721 tgtgacttct cctgcccgaa gcaatgctgc aagctcgtga atggtcatgt cgcacagctt
    90781 cattcgtctg ccccctctgc ctcgattatc cttggcaccc ggaagaactc gccctgcctg
    90841 tcgggggcat tggcaagtac ctcctcacga ttgagcgagg gcagaaccac gtcctcgcgc
    90901 catacattgg cgaccggaca aggttgaatg gtcagcgcca ccccgtccgt atttagcccg
    90961 gcaattttgt tcatgtaatc gattatctca gaaaggtcat gttgtgcctg agcaagttct
    91021 gattcattca ggctcaacct agccaattcg gcaatatggg ctgtctcctc ctttgaaatg
    91081 ctcaattcac tcaccaccat cacaaggatt atcgttcatc accaagacgg catcccgccc
    91141 gttccaacat ctcagcaaaa tcctgttcag acaggacagc aaccccaagc tccctagctc
    91201 tggcaagttt ggaccccgcg ttctctcctg ccacaacata atccgtcttc cggctcacac
    91261 tcgaacttac gcgcccgcca aggttctcta ctgcttcctc cgctttctgc cttgtcaggc
    91321 tcgcaagcgt acctgtgaaa acaaaactct tgccgtcaaa ctcgacagcc gccggcttgg
    91381 cttcttcagg ttcatccgac atacgcacgc ccgctgcagc caacttcttc acggtctcca
    91441 ggttcatctc agatgagaaa aacgagacta cgctgtcagc gatcgccggc cctattgtag
    91501 gaatcgcgag cagatcgtca cgctcggcgg cgataagcgc atccattgaa tgaaaatgtg
    91561 aagccagcag tttcgccaca ttctcaccca catgccttat tcccagggcg aaaatgactc
    91621 ggtagagcgg attcgacttg ctctgctcga tagaagccac gaggtttgcc gctgacttct
    91681 cacccatcct atccagcccc gcgagctgat ccaacctgag cccatatatg tcagctacat
    91741 cagcgacgag tttcgcatcg agcaacctgt ctattgttga cggcccaaga ccttcaatat
    91801 ccatggcgcc tttcgaagca aaatgaatca tcccctcacg aatcttcgca gggcatgata
    91861 gacttatgca gcgcgtagcc gcctctccag cctcccgtag aacctcagaa ccgcatgcag
    91921 ggcatacccg cggcatctca aattcctttt cgtctccagt tcgttcttca accaataccc
    91981 gtactacctc tggaatcaca tccccggccc gctggattat aacggcatcc cccacacgca
    92041 cgtcctttgc ccgcacataa tcctcattat gaagagaagc gcggctcacg gtgactccgc
    92101 cgacatcaac aggctcaaag actgcggttg gagtcaaaac tcccgtcctt cccacctgca
    92161 ctattatgtc gcgaactcta gtttgcacct gctgtgcggg gtatttgtat gccgaagccc
    92221 acctcggcga atgcgaggta aacccgagct ttcgttgaag atctagcctg ttgactttta
    92281 caaccacccc gtctatctcg tatggaagca tgggccgcaa atcggccagt cgatggatga
    92341 actccagagt ctcccccaag ccgtcgcaca agatgatctc gggattcacc ctaaaaccca
    92401 atgagcgtat aaaatcaagc gcatcccaat gcgtctcgat gccgtaacgc tctgcctccg
    92461 gcagatagta gaagaatgcg tcgagcttgc gggacgcggt cactttcgga tcgagttgtc
    92521 gcaatgatcc agctgcagca ttgcgtgggt ttgcgaacat aggttcacct ctgctcgccc
    92581 tttcttggtt gagccgttcg aactcggatt tgcgcatgta gacctctcca cgcaccgtaa
    92641 ccgaatcggg agcgcctttg ggcaacacca acggcacagc ccttatggtc ctgagattat
    92701 gggttacgtc ctcgccgcgt tcaccatcac cccgagttgc cccaactgcg agcctcccat
    92761 tctcgtatgt gaggctgatc gcaaggccat caattttcgg ctcggctacg tactctacct
    92821 tggccaggcc agtcgccctc tttatgcgcg cgtcgaacgc ccgcagcgaa ccctcatcga
    92881 acatgttgtc cagggacaac atggggattg catggaccac cggctcaaag ccggccgaca
    92941 cagcgccgcc aaccctctgt gtgggggaat caggatcagc aagctccgga tgagcctctt
    93001 ctaaggcaat cagttcgcgc atgagcatgt cgtactcggc gtcggtgatc tccgggtcat
    93061 cgaggacgta gtatctccag ttatgatgac gtatcagttc cctaagctcg tcaactctgc
    93121 tggctgcgtc agtcaactca gccacccccc tcggtcgcta gattctctcc atgggcgcaa
    93181 agctctgcgc caaatgcttg attccccctc cggggaacgc cactgttatg acccaatcat
    93241 tcccgcctct tgccacggcc acaaccgttc cttcaccgaa cttggcatgc ctgacgcgat
    93301 caccggcaga atactctgca ctaccgtccc cgccgtggtt ttcgcctccc ccgtcactgc
    93361 tgtcgccccg gttccatgct gtcccagacc gcccttccac ggagtgagca cgtataaccg
    93421 gcgaagcggg cccatccgat ctgccccaga ggcttcgcct atcatgcatc gcatcagcca
    93481 gcagctcctt tggaatctcg cccagaaatc gcgacggcgc gtttgccatc gtcgaaccat
    93541 agatcatgcg catcacagaa tgcacaaggt atagcctgtc ctttgcgcgc gtgatcccca
    93601 cgtaacaaag acgccgttcc tcctcgagag agcgctcctc gcgggcggat cgcgcaaggg
    93661 gaaagatatt ctcttccatg ccagcgatga aaaccacggg gaactcgagg cccttggctg
    93721 catgcatcgt catcagtgta atggaatcat ctgaaaggtc tgccctgtcc agatcggtaa
    93781 ggagtgagac ttcctcgaga aaagctccaa tatctgtcgg tccaccgcca tcctcaacag
    93841 aattctccat acgcctggcc tcaaattcct gagtcacggt gagaagctct ctgaggttct
    93901 ctattctgcc ctcggcctcc agagtctgtt cccgttccag ctcacgaatg tagccagtct
    93961 tttcgaggac catctctaca agctgagaga ctgtagcctc ccctttgcgc gattggaggc
    94021 cctccatcaa ctggagaaac tgcgacaacc cgctcctggc tcggctcgga gtatccgacg
    94081 ctcggaccgc caattcgacc cctgcgtaga gtggaactcc ggcctcttct gagagtctgc
    94141 ggactatggc aagtgtagca tcacctattc ccctacgcgg aacattgatt atacgttcaa
    94201 gggcaaggga atcctcgggg ttctcaatca ctcgcaagta ggccaggacg tcctttatct
    94261 cgcgccgctc gtagaacttg aggccgccga cgatcttgta tggcatgccc ctattcaaga
    94321 ggacttcttc tatcgccctc gactgcgcat ttaccctgta tagtactgca aagtcgcgat
    94381 acgcccttcc ttcaagagct gcaagccgct gaatctcgtc tgcaatgaac cttccctcat
    94441 ccctttcatt ctcggcacag tacacaaaga tcggagagcc cgaacctcgg gaagtccaaa
    94501 ggctcttgtc cttacgccct ttgttgttgg ctatcacgca attcgctgca ttaaggatgt
    94561 tctgggttga cctatagttc tgctccagct tgatcacacg ggcttcggga taatcacgct
    94621 cgaaatccag gatgttgcgg atatcggcgc cgcgccattc gtatatcgat tggtcgtcat
    94681 cccccactac acacaggttc ctactccttg ctgcaaggag tttgacgaga atgtactggg
    94741 catggttagt gtcctgatat tcatctacca tcaggtattc gaatctgtcc tgccatttct
    94801 cgagaatctc gggacattcc cggagcaacc tcactgcaag ggttatgaga tcgtcaaaat
    94861 ccagagcatt gcctcgaaca agcgtgctct gataagccgc atatatctgc cccatcgtac
    94921 gagttctggg atcgcgtgca ttggcctcaa gttcctcagg acctatcaac tcattctttg
    94981 cagagctgat ggcagcgagc actgcctgcg gcttgaacat gtcgccggaa atgttgaggt
    95041 catcgagaca cttctttacc acggccgttt gatcagattc atccagtatc gtaaagctct
    95101 tcttgtaacc aagtgagggt atgtcctctc tcaacattct cgcgcacaaa gagtgaaatg
    95161 ttgatatcca catgaggtcc gcacgcgcac caacgagttc ttctactctg ttcttcattt
    95221 ccttggcagc cttgttggta aacgtcacag ccagtattct gtatggaggc acccttcttt
    95281 ccgatatcag gtaggctgct ctatgcgtga ggaccctggt ttttccgctc cctgcgccgg
    95341 caagaatgag aagcgggccg tcgacacaaa cgacggcctc tctctgcggc tcattcaatg
    95401 cgccaaggag atctccgccc atgcccctat tcctccaaaa gcgctatggc ttttctgatc
    95461 cgggccaaag atcgatcccg gcccagcagc acaaggctct cgaagagagg cggggtgact
    95521 cggcggccag taacagcaat cctcacaggc atcagcagct cccctgcttt ccagccaagc
    95581 tcagttgcgg tccctctgag tgcctcctct atcggctcat gctcccacgt cgataccttc
    95641 tcgagcgcgt cagcagacgc cttaagcccg cgcgctatgt cgtcgtacga aaacttggct
    95701 ttggtcttga gagcgctcgg gtcaagttcc acgtcgtcga cgaagaggaa tccaagcaaa
    95761 tccggcgcct cggtgagctt gcctatcctg tcggtgatga gcgccactgc ccgttccaga
    95821 tagtcggtat cgtcaaccga atagcctgca gcctccataa aagggcgaac tctggaggcc
    95881 aaatcaggca cggtgagact cctgatgtag actccgttga accagtccag tttcttcatg
    95941 tcgaacacag cgtccgattt cgacagcgtg tccacggaaa atgcctccac cagctcatct
    96001 atgctgaata tctcttgttc agtgccgggg ttccagccca gaagggcaag gaagtttatg
    96061 acggcctctg gcagataacc catttccctg aattcgccaa tatacactgc gccagccctc
    96121 ttcgagatct tggtgcggtc agggttgagc attagggtaa ggtgtccgaa ctgaggtggc
    96181 tcccatccca tcgctgtgta tacgcatact tggtcgaaag tgttggtcag gtgctcgact
    96241 gccctgaata cgtgactcat ctccatgtaa tgctcgtcca caacagtggc gaagttgtac
    96301 acaggagtgc catcagactt gaatatgatg aaatctcccc gctccgacat gtctttctgt
    96361 tgatgcccgc gtataagatc gtcaaaagac aaagtgacat cggggacacg aagccttacc
    96421 acaggccttc ttccttccgc ctccagcctc gcgcggtccg cactactcag ttccctgcac
    96481 tttccggaat atcgaggagg ctttccggca gccagcgctg ccttgcgctc ttcctcaagc
    96541 tctgaagggg tgcaatagca gtaataggct ttgccctccg aaagcaattt ctccacagtt
    96601 tccttgtagg tatccatccg ttccagctgg cgatatggac cgtaaggccc tcctatgtcc
    96661 ggcccttcat cccacttgat tcccagccat ctcaagcctt caaggacgcc ttcttctgac
    96721 tctttggtgc ttcgttcaag gtcagtgttg tcaattctca ggacgaatgt gccgccgtat
    96781 ttccgcgcaa aaagcatgtt gtacagagca gtccgagccg tccccacatg gcaattgcct
    96841 gtgggcgatg gcgcgatcct aacccttact tttctatctt tagacgatgt tgagtccgca
    96901 ttcatggatc aaaagtcagc tcctcttcgc tacgcagtcg tgcctatgaa atttagtttt
    96961 gccgtcttga tagcaggcgc ccggtatatt ccgcggcccc ccgcaaccgg caacagctct
    97021 gtgtcgcgcg acacagcaac tatgttcgca agaattccgt cgagtattga atcagtgaac
    97081 ctcagattcc gcagcggccg aactattcgg ccgtcttcaa taaggtaagt gccgtcccgc
    97141 gtcatccccg taaaaatcgt tttcgtcgga tgcaccacat tggtgtagtt gaaccgcgtt
    97201 acgtaaacgc cccgcccggt gctggccacc atttccccaa tggacgattc cccggggctc
    97261 atccgcagat gcagtgggta gccgcctttg agggcgcccc caacaagggc atgcccggtc
    97321 gactctgtcc ccgctttctt ggctgcaagt ctgtcaaaga tcatgccgga cgccacgccg
    97381 tgttcgataa gggtcacggg ccgtcgcgga accccttcca gatcgaatgg ccgcggcaag
    97441 aggcccgggt catgccagtt atcctcgata gtcaccatat cggacgtgat ctttgaacct
    97501 atccgtccgc tagcataact tcttccctct atgaatgata tcccattgat gcccatcaaa
    97561 gccagggtct ccatcatgtc gactactgca gctggctcaa gaatggcttc gtattcgcca
    97621 ggctctaatg caccaggatt cctggaagaa aggcacttct ccacagccct ttctccgata
    97681 gcccgcgcgt caagattccc cgctgaccag gacgccgctt cagcgtagcc tgatccggca
    97741 ttggaatcgt atcccgtcat gatcaccgcg cttattgcgg aatctgtccc tctcatccac
    97801 gcgctggctc cttcagagtt tgcaatcgcc atgccagtaa ccctcgtacg aaacgccccg
    97861 gaggcgttga gttcatgctt gtcagctatg gctgtgattg ccttcacagc catggctctc
    97921 tcgcgggccg aaaaggatgc cgttcgctcg tcgtagccgc cagcttcctc atatctacac
    97981 agccctgcca aacccggaaa atcgggattt tcaggttgaa ccatggcgat ctccaccgcc
    98041 agcgccgcag cgtccctaac agcttccgag gtcagctgat ttgtagtcgc ccatccctca
    98101 cgttttccac aaatggccct cacatacaag atgtctgacg agaccgaagc gttctgatgg
    98161 atctcagaat ttgcaaacct ggtaaggttg tcaacgtcgc tcatgagtac agcttcgaca
    98221 gcatcagctc tgcaggctga aatggccgct tcgactatct cctgcgcacg ctcctggcct
    98281 accatcttcc gacacctacc ttcacattcc tgaacctcgc aggcgcagtg ccatgcccaa
    98341 cgaacatgac ctggctgggc tctccctttc cgcagttagg aaagccccac agcacccatt
    98401 cgtcagaccc cgcgatacca tcgcaggaat tccaaaactc tggagtaatt cctgtgtatg
    98461 ttggattctt caccatctcc gcaagctggc cattgcgtat ccgccagccg atctcgcttc
    98521 caaattggaa gtttaagcgc ctgtcatcaa tgctccaact ctttatcccg tccatgaata
    98581 tgccatcatc agttccgcgg atgagggatt cgagggtctc gttacccggc tcaaggcata
    98641 tattggtcat ccttatgatg ggcattctat cccatccgct cgctctcata caaccattgc
    98701 tggtctttcc gagccttgca gcagtctccc tcgacatcag atacgcctcg aacatgccat
    98761 ttctaaccag atcggttcgg caagaccgaa ccccttcatc atcgtacgca aacgtaccaa
    98821 gccctcctgg aatcgtcgaa tcggcgtaga tgttgaccaa gtcagatcca tacctgaagt
    98881 ctcccagctt gccaggatca agaaaactcg ttcccgcata agacgcctca tagccataga
    98941 ctcggtccag ctccgtcgga tgaccgcaag actcatgtat ctgcagtgcc aactgattgg
    99001 tgcccagtat cagagtggtc tttatctgcg ggcattgttg ggccgcgagg agagcctgag
    99061 cttgtttccc gatttcagcc gcgtgtccag caatgtccat ctcctctatc cattcgtacc
    99121 ctgcctgcct cacattgcct tcaaacgtcc caggatacga tctgacctgc atctctccgt
    99181 tgccaagagc tatcgccttg atgcccgccg aagtgaccgt ggtccgctgc cgaatccgcg
    99241 ccccctcaga gttggcatag gccttatcat cccttatcgc attcatggac gccgatgcat
    99301 agcgcacatc gcttccgccg gacatcgcgc ggctgcaagc ctccctgaga aggtctatct
    99361 tctgagccct cggaactgac caaggatcta tggcacatgg cgactcccac tccacaacta
    99421 caggatcgag tggcgcaaga acaacgtcac acttctttgc cattgcgctt gccctggcaa
    99481 tctctactgc ctccgcagca actctggcag cctcgctctc gctcacaaca cagcttgcgg
    99541 cgaatcccca tgccccatcc acaagtgccc taacgcctat accatgcgag actccgctcg
    99601 tggcccgctc cacaacacca ttgcgaacag agagcctctc ctccgtctga tgcaacgcac
    99661 gaacatccgc atattccgct ccggtcgcgc aggctctgtc tagcgctatt tcgagaactc
    99721 tcaagccatc acacctccct ggatcgtttg atatcaccaa cctgacacat cagaatgccg
    99781 gctagtatga gaatggcccc aatcgcctgc tgcacgctga gacgctcccc caaccatagc
    99841 cgcgcaaaga tggatgtaaa aatcggttcg gtctggagta tcagggccgc ccgtgtaggc
    99901 ggcgtatatt tttgagctgt agtctgaacg agataagcaa gcgctgttcc tgttgcacca
    99961 aggtaggcta cggagaggac taagtccttg tcgaattgga atatctggac tatgtcggat
   100021 cctgcagcca acaccgaaaa gaccgcagcc catgccatct gggtaaatga caggagcaag
   100081 ccgtccatgg tcggcgaata cctggctagg aggattatgt ggaccgcaaa gcaaaatgcg
   100141 cacactaaga tcagaatatc gccatatgtc ggaggcgaca gcctgtccag tgtgagaagg
   100201 ccaagccctg caactgcaac accaacgcct gtgatctgat atggcatgat gcgattcttg
   100261 aggaccttct gttcaaggaa aagcgtaaaa actacgtgca tccctgtgat gaacccggcc
   100321 ttggacgcag tagtcccctg caacccgcaa gtttgggtta aaaacccgag catgaggggg
   100381 attgccagcg cgaaccctgc cttacagtat tcccagtcca gtccgcgaag gcgccgaccc
   100441 gcaacgagcc cgagaagaac cgtagcaagt acaaatcttg ccgcaataaa agtggccgga
   100501 ttcgtgtcgc ccaccactgt cttgactatg gcgaagctcg ttccccatat aacgcatatc
   100561 cctactaggc tcaggtcggc caggattgat ttgtacgacc ccaagcgcca tgatttcaac
   100621 tgctgtccct actcccacta agcgattaca gacacctaaa cgttccgatt atgtcgcaca
   100681 aaagcaccat tatatatccg aacagcacct ccccactccc agaacttcgc agaagccaaa
   100741 taccccttga gttgctatac ctacaactga tatagccaat ctactgccgg tgccagtcgc
   100801 cccaatcctc tttcaggaag tcagtgcaac ccgcatccca atgataccca ttgcagcttg
   100861 catcggaata ggcattgtat tacatggctt tgggatctag caacattnnn nnnnnnnnnn
   100921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   100981 nnnnnnnnnn nnnnnnnnnn nnnnnnnctc cggcacatgc agctcaattc tttgccgtta
   101041 cttgtcacag gaatccacaa acgatttcac gtgttccaaa tactgcgggc tcaataccag
   101101 ctgcctagct tcttctcccc aggcagggaa atcaggcttt tggtatgcta aaatgaccgt
   101161 gatcaacggc gtcacaatcg atgtgatcct ctcttcgttc tgacgctcgc attctcttga
   101221 tactccgtat tttccatggt aaagcatatc atactgatct tcaaaagccg ttcccgcttc
   101281 gactgacaaa ctagacttca cattcgaaat atcgtttccc cgatagcctt gacctagata
   101341 attgtaatca aacatcatgg cggcagtgtg atcttttctt acgagcatgt tcgtccaata
   101401 aaagtcgttg tagttcaaag ttcgcaccaa ccccgccagt tccttttgga acaggcctac
   101461 atggttttgc agataacagg taatatcctt aagagaagga tccctttccg acagacgatt
   101521 gaggtttgct atcgtcaggc aatctgtctc acagtacagt tcaggcagct caggaatgtt
   101581 gatccccttc tcgtggaaat gaaaatacca ctcggctaac ctgccggcaa tttgctcatc
   101641 tctgagatcg tccgggatgc ctaaacgcca cgtcttgctc tcagcgatgt cttccaaaac
   101701 aagacatcgc tgtgcatact cgagaacgtc catcgtcgga atgcccaagc gatgaagaag
   101761 ctgataatta tggatctccc tcaggtcgtc atctttttca aagtacttga ttacagtggg
   101821 gcttccatcg aaggaacaac gaaaaaccca cacccgatcc ttgctgcgaa tcagctcaaa
   101881 ccgatcaatc tcgtgtgagt ggagttggcc taagatatgg cgcctaaccc gttcttcagc
   101941 atcctgcctt ggttctccag cgatcgctcg acccatcctt ttgcttctcc tcatctaggc
   102001 gatcgtccct caagatccgc gccactgtaa atctccttag cttccatcgc ctagaatgcc
   102061 aaatgctcct tcatgcaaat tcaatctcta tgctaataac gtcattatac catgaatccc
   102121 tgagatccga ccccgggccc aagcagttag cccctgcaca tctcggctgc gcaaccacgc
   102181 actctgcgat tgccaaacac acccgttttc ggacgtgcag ttcgatgcgc cctgtgatac
   102241 tatccatgtt gctggcccct ttcgcgtgta gctctgtgtc aaggtaggct cttcccctgg
   102301 gtatatccta ccgatcgcga tagatacctc attcacggcc ccagaaagtg agccggaagg
   102361 ttgtcacaat agcgtcccac taatccgttt tgaacagctt tctggcatcg ttcaaacggc
   102421 agaaatcgac gcaaaatgca ctgacccaga gcgtacgcgc cagaaatggg cctcagtcta
   102481 gtccttcaag aatctcctcc catttgtcat gaagattccc tattgaggag gattgtatgg
   102541 taatcacaga cggcagagtg atcctgagag attttgtgcg agaggacatt gaagacagga
   102601 ttcgctggga gacagtagag acagaatggt tactatggga cgctccatgg gagcttgaag
   102661 aggaagaacc gttttcgcct gacgcatatc gagataagat gctgcgccaa ctgtccacag
   102721 aaaaggatcc tagtagaatg cgccggagtt ttcagatatg catcaacaat ggaactcgaa
   102781 cgcacattgg ttggtgtaat tcatacaaca tagacgcgaa ctatgactac acagatgagg
   102841 atggatactg cacgatagga atcgacattc ccgagcaaag tgcaagacgc cagggttatg
   102901 caaccgctgc atggaagctg ttcatcgaat acctgctgtc caacggagtt gaagacattt
   102961 acacacagac ctggtccggg aatgagcgcg tcctgggcct gataagaaag atgggatttg
   103021 aacagtgcgc tcgacaagtc aattaccgga tggtgcgtgg ccaatcatat gatggactga
   103081 cgttcaagct gaacatttcg agttttcgaa agctcgtata tggcaaatag cgcccacccg
   103141 tggctgagct aagcccttca tggattttct ctgatgccgt tacttgcatg cgcagcatca
   103201 gctcgcggcg tatgtttttg tgaaacctgg attcagcgca cctactgttt agccggtcat
   103261 ataacaagcc atatggaata cgccacatgt cagatgtgaa attcaggttt acctgtcctc
   103321 ctgattgcaa ttccagccct aggcaaaata cgacgccggc ctcctatgct tatcccatgg
   103381 gaagccgacg ttctgtctat attcattccc cagctctgaa attatccgcc ttttggatta
   103441 catcgaagca aatggaggcg aactagaagt agagccgtcc taaaaagcct gaaagagtct
   103501 gttttgcctg ataggtgcaa atctgcacct cattcccact caatagtggc cggcggcttt
   103561 ggagtaatat cgtagaccac tcggttaact ccgggcactt catcaacgat gcgtcggctg
   103621 atctcctcca atgcagcata cggaagcctc gcccaatcag cgctcatgcc gtctgggctc
   103681 ttcaccgccc tgattgctat tgggtaggaa taggtgcggt cgctgtcgct cactccggtg
   103741 ctgcgaatcg cgggaagaac cgcgaaatac tgccagagct cgcttgcctc aggaagcttc
   103801 ccgatttctt cacgcactat ggcatcggcc tctcttaaaa cctccagccg ttctcgggtg
   103861 acctctccaa ttatcctaac agccagcccc ggcccgggga atggctgcct cttcactatg
   103921 gtatcaggaa gccccagttc cttgccgatg aggcggacat catccttgta gagcatcctc
   103981 agaggttcta tcagttcgaa cctgagatcc tccggaagtc cgcccacatt atgatgggac
   104041 ttgaccttga ctccaggttt ctggctagag acactctcaa ccacgtcagg aaggagagtc
   104101 ccctggacca aatatggaac atttcccagc ttttccgatt cctcttcgaa gacccgtatg
   104161 aactctgcgc caataatctt cctttttgcc tccgggtcca caacgccttc aagcttcttc
   104221 agaaaccgct cttttgcatc cacaactatg aggttcatgt ggtaatggcc gcggaaaacg
   104281 ttctccaccc cttcagcctc gcccttccta agcagaccgt tgtccacaaa gatgcaggta
   104341 agattggcac ctatagcttt atggacaagc accgcagcaa cagatgagtc gacgccgccc
   104401 gacaatccgc aaactgctcg ctccgctcct atctgctgcg aaattgctga aatcgcctca
   104461 tctatgaatg agcgcgggtc gcggccatcc tgtagaccgc ctgcattgtg tagatgtctc
   104521 tctttcatga aatttcaatg ctcctcgttc cacacataat ctttaggcac gcccacaaaa
   104581 ggcaggtttc ggtatttttc catgtaatcc aagccataac ctacaacaaa cttatcggga
   104641 attacgaatc cattatagtc cggaacaata gacgcctgcc tccgatccgg cttatccaga
   104701 agtgtgcata ccttcacaga tgctggcttg cggttcttca gagtcgaaac caagtaactg
   104761 agggtaagcc ctgtatcgac tatatcctca acgatgagga cgtgtcggcc ttcgatagga
   104821 acatcaagat cctttattat cttaaccact cctgaggaga cagtcgatgc cccatagcta
   104881 gtaacggcca tggtatcaat gctgaccgga atcgatatgc atcgcatcag atccgacatg
   104941 aatacaaggg cccctttgag tatgcacacc aacagtaaat cctttccctc gtattccttg
   105001 gatatttgtg cccccaattc cgccactttg gagcctatct gttcctctga gatcaaaact
   105061 tcttgaatgt cttcgatgat atgcattgaa ggatgcacag acactagccg attcctccca
   105121 tcacgtctgc aggccggccc gaagacctca tacggaccgg cctgctcatt aacatattga
   105181 aacaggcaaa tcacagcaca ctaccggagg aaagctatcc ccctggcctc atgcacaaat
   105241 tccttcggca cagagatcgt gatgacagca gtcttgttga aagccgcagg tttcctgaca
   105301 tctacgactg tcgcgtcgca tatttcccgt ccagctctgt cagttgcctt tacgacggcg
   105361 ccgcgctcag gaagaggcaa gtattcatag gggagcgtca ccttggcctc atcgccagat
   105421 cccgatagat cctcgataaa gatggcaagc ccaggacaga caggaacgca gttcccacat
   105481 cccctgcact tctcctcatt cagcacggga aggttcgtta ttcgatcccc aacggaaata
   105541 gcgccgaatg ggcatcccgt ttcacacggg ttgcaaggga tctcctgaag gcattcgatg
   105601 accgccactg ggcctttctc agcccttgaa ctgcttggac atcccttcga agccatgacg
   105661 tcttcccact taacaatacc gtccttctcg acgtccttca gcaaaccact cactcctctg
   105721 gcccaccacg agcccttctt atgactctcc tgtcatagac ctttggcaca acgtcaagga
   105781 tggaatttcg tgagcaatat ctcacatcct gctcgtaacc gattccaacc agataagctc
   105841 cgtggtcaca gcggagcaac tcatcctcaa tcgacccttt tcgctgctgg tacaagagtg
   105901 aagccgcctt ggcagagtcg gacatctccg ctcccttgtc atgtgcaatc ctcgtagcca
   105961 taagtcctgc gcaaagcgtg tcttccatgc agaacctccc gtcttgcccg gcacagacca
   106021 gcaccaagtc gcagtcgtca taggcttcgc atgctcctat aacagcatca gcgttaagaa
   106081 aacacgctgt aaaaatctct cgcgcaattc catacttatt gatggcgcta gtgccatttg
   106141 tagtcgccat gatgaccttc ttcccagcca ctcgggggcc atcatattct tgcggagaat
   106201 tgcccagatc gaatccttca acaggaactc cccgccgctc ccctcctaga acataatctg
   106261 acctcttgta accattggcc agggttatgg cctcctctat actctcagcg ggaataacct
   106321 cctcgcaacc agaagccaac gccgtagcaa tgctgctggt agccctgagg acatcaatta
   106381 ttaccactac agacccagat acatccttgg ctgcaatctc gcctgggacc agtgaaacat
   106441 ctatccgcat tgcggttctc ctttcatttc tgtgcagaat cttgaagtga tcgaacgagc
   106501 gcctcaatgc cttgagtatc cggatcccaa taagcaccct taaaataacc tggcagcgta
   106561 gcagaattta tggtgtcaga atcaagcctt ctcaaacgcc aagccacggc agccacttcc
   106621 ttgacagtca tatcagtgtc ggcgcacttt tgggctacgc gcagcatgtt cggataagat
   106681 ggaatagccc gaaagctccc agcctcccta ataaacgcct tcacgaattc ttgctgacgc
   106741 gcagttcggg atatgtcccc caacccatct tgccggtatc tgacgaaact gagagccatt
   106801 tcaccatcca tgtggtagac tccaggctcc aaatctatgg aatacccgcc agccttgtcc
   106861 tcatatttca tccgcttatc gactgctacc tccacaccac ccaaggcgtc tacgagttcc
   106921 acaaatcccc caaagtccac aacgacatgt ctgtgcaccg gcagccccac gagatccgaa
   106981 actagctcac gagcgagatc caccccccct tccggaacag aagagttaat ccgccttacc
   107041 ccgccattgg ggaatcttac cagagtatcc ctaggaatcg atagaagtgc cgcaccccgg
   107101 gaactcttcg ggaccatgag caacaggata gtgtcgggcc tgcccggcgc agacaagacg
   107161 tcgccattac gggagtagtt acggtcttcc cccaaaatga ggatgttaaa gagcccgttc
   107221 tcggcaggca aatatgtgaa gtatagaaaa agaccacaac acgcagctac caccacaatt
   107281 actatcgtcg cccagattcg gcccgtattc gaacgcccag cattggcatt ctgtctcaca
   107341 tttctttcac caacctcaga catgacacct actccttaga cgcaccgaag cactgattaa
   107401 ttcatttcta cagggacgcc tcgtatcctc ctgaaaaacc tagccgtcaa agcaggcgag
   107461 gctgctcagc aaggcacctg gttcagcatt cgctgacagc gatgacacct ctccagccac
   107521 atgtctgtag taccctgcag aggctatcat ggccgcgttg tctgtgcaca gattgagtgg
   107581 aggatagtat acttctatat tgctcttccc agccagcgcc gtcaatgccc ttcgcaatgc
   107641 ctgattcgct gcaacccctc ccgaaaccgc cacaacactc atcccggtga aatccactgc
   107701 ccgatccacc ctggagacga gctcatctac agcggccttc tgaaaacccg cagccacatc
   107761 agcatagtca aggtcttcgc ctctttgttc tgcggtatgg atgtagttga ttaccgccgt
   107821 cttcaatccg ctaaagctaa aatcgagcgg actcccttct acctccgctc ggggaaacga
   107881 aatcagattc ggatcgcccg acttagcaag ctgctcaagg gcgggtccgc caggatacgg
   107941 aaggcctatt actcttgcaa ccttgtcaaa tgcctcgcct gcggcgtcat ccctcgtgcg
   108001 tcccagcact tcatagcgcc cgtgatctcg catggcaatg agttccgtgt gtccgcccga
   108061 caccgtaagg cacaagactg gaggactcag ttggggatgg gcgaggaagt tagcatatat
   108121 gtgtccctca atgtgattca cacagatcag cggtttccct gcagcgtacg atattccctt
   108181 ggccgccgaa aggccaacta gcaaggcccc gacaagaccg ggaccatgtg ttgccgcaac
   108241 tgcatcgatc tccgccagat cagttccggc ctgcgccagg gcctcatcta taaccggaat
   108301 gatcgcctcc acgtgtcgac gcgaagcgat ctcaggcaca accccgccat atctacggtg
   108361 aacctctatt tgagacgcga ccactgatga gagaatctcg cggcccccgc gcactaccgc
   108421 cgccgccgtc tcatcacaac tagtctcgat accgagaacg accccgtcct cacgcatcct
   108481 ccggggcacc tccctcgcca tccatcattg ccaagcgtcc gggcagatcc tcaagccaca
   108541 tcaccagggc atcctcatgg tcatccaagt agtaccgcgg cctcacgcca gctgccacga
   108601 aacctagact tcggtaaagc tcctgagcgg gcacgttcga caatctagtc tccaaagtca
   108661 tcctcctagc gccccggagg accgcggttt ccatcaaggc aaccatgata gcacgtccaa
   108721 ccccacgccg tctatattcg gggtgaaccg cgacattcgt gatgtgggcc tcgtcgagga
   108781 tgagccacat cccgccgtat cccattgcaa ctccatcgga gtccctcaca accatataca
   108841 cggcttggtc attcatgaga agctcatcca cgaacgcatg cctgctccat ggaagagaga
   108901 atgacagacg ttcaatatcg agaactctgt caagatcatc aagattcatg ggagacggaa
   108961 tcagatccat ccttttccat ctccttccgg gagagcttta cctcggcctc ggtcggacga
   109021 aggtacatag gaagcacatg ggctggatcg ccgccttctc cgtgttccag catctccatg
   109081 cctaacattc ctacctgggc cggtctgaga atcccgaacg ccgctggcgg cacaaccccg
   109141 cgtccaggaa aagccgaaag caaagagtcg gaatacgcaa gggcaccatc gccaacgaaa
   109201 agaacacgat ccccgtcagc gcctgctgaa tcaactatct gagaaacatc aaggacagaa
   109261 tagtccaata tccgttttgc agcgccgaag ctatcagaag gcttgtaaca ggccacgtag
   109321 caacgcccat ggcgagcatc tatcatcgac cacacttcag aaaacccggg gctacgcccg
   109381 taagcaaggg catcaagagt cggcactccc accaacggaa ttccgagggc gtacgacatg
   109441 cctttggctg cagacacccc tatgcggact cctgtaaacg aaccaggccc tacaccgcat
   109501 gctatggcat ccaaatcacc gggagcaatg ttggcgcctg taagcgtggc atctactgac
   109561 gacataagcc tctccgaatg agtagctctt acgttcagaa catgttctcc taagagggct
   109621 ccatcattga tgagagcgac gcccccaacc atggtagacg tatctacagc gaggatcagc
   109681 acggcctcat ctcctccagc aattgtcgat atctctgccc atatggagtc aacacaagct
   109741 cccgcctgag actgaccggc tcctgagtaa tctccaccat aagcctgtca tgctccctca
   109801 atggaacata gactccaggc cattcgatga tggcgactcc gtcgccccag atgtattcat
   109861 caaggcctgc ttcttccact tcatcgcttg aggacagccg gtaaaggtct agatgatata
   109921 ctctaaccct gccccggtat tcctgtgcaa gcacgaaagt tgggctcgat acagatgttt
   109981 cgtcaacacc caatcccttg gcaatccctc gcgccaaaca agtcttgcct gcacccagac
   110041 cgccttccaa gcaaacaata tccccaggcc ccagcagtct gccgagcctg catcctaaag
   110101 cggcggtatc agcaggcgaa ttcgacataa acttcaagtt ttcaccctta ttcatgctca
   110161 atccgcataa cctcgcaatg tgcctgtgct gaccctatta gccgcaagat tatactccac
   110221 tcccccggcc aaggtcaaac cgacaaacga gaattgcctt ctgcgagctc aaagcatgga
   110281 gagatctccc caacaacgtc ttcccggcaa ttgcggacaa acgccacaat agatgtctgc
   110341 ggctcagcga acgtctcttg gggaaccaca tccaataggc gcagagtctc cataactgca
   110401 gtctgcaacc ctctggatcc tccgtcttcg attttaacag ctatccctat cccgcgtttc
   110461 ggaacaccta cgcagaaaag gccctcagcg ccgcccttgg cgaataccgc gcctttgcta
   110521 ttcttcataa gattagatcc gaaatcgagt gtgcccccta tcatctccgg gtactccacc
   110581 attgccgaag tgacgcgccg agcaggctct ctcaaacgct caggcaacat gtcggggcag
   110641 gctagccgtg cgtaggcgta ggccatgtta gcgatgggca tcccaaagac cacaacaccg
   110701 cacccatcca ctccgcgcac aactttttca ggcaaaagcc cgcagatctc agcaactgcc
   110761 ttccacatat cgatctggac caagctctcc ttatcaacgt aattgtcgat gggatatccc
   110821 ttgtgcgttg cataggcaag catcccggcg tgcttgcctg agcaattatt gaaaaccggc
   110881 gtgacaggga ttccttccct agcaaggcgg gcagcctgtg tcgggttggt aggcttgtgc
   110941 actccgcact tcaggcatga ttcatctaga cctatcttgc ccagaatgct cattacagct
   111001 tccacatgac aatcttcccc atgatgggag gccgccataa ccgcaagctc tctctcggta
   111061 atgccgaatc ggtcaaccgc gccagactcg ataattggca acacttggaa cggtttagcc
   111121 gaagacctga aatacgtaac atacatgggg tctcctgcct gccacagcac attgccgcgg
   111181 caatcaacta cagctatgtg gcccctgtgg aggctttcaa ccagttctcc tctggtaact
   111241 tgcaccagcg gcgcagacat tgtaggcaat tcactacact cctcgtacag cattgacgaa
   111301 ttcggtgaat atgcgcaaag actgcgcatc cctctccgcg agaccttctg gatgccactg
   111361 aacccccatt acaaagcggg aaccgggttt ttctatcgcc tcccgaactc catcgtcagc
   111421 cacaccggtc accgaaaatc ctggtgctac aggaccgacg gcttgatgat ggaagctgtt
   111481 tacccagatt gtttcttctc caaatattcc gaacaatata gaattgcgag caagcatgac
   111541 tcgatgggtt ggatgccacc ttggcgcaga aacccggtgc gcatgggcac catctacgca
   111601 gctggcaata tcttggatca gtccgccccc cgctgcaaca tttagtatct gcgctcccct
   111661 acatatcccc agcacgggga tatcgagctt cagtgcggct ttcgaaagag ctatttcgaa
   111721 ctcatcccgc acaggattga tagcgcctat cttgggatga gcatgggatt caccataaaa
   111781 cctgggatca acatcatctc cgcccgtgag cagcagccca caggcatcgg acagaatgcc
   111841 atgggcaact ctgttggagt cggcgcaacc cattggaggt gccaagatca aaggcatccc
   111901 tcccgaccgg tagattgcct tcgcgtattc gactcctaca gcgattttgc cgttggtccc
   111961 ggactcaaag tcgctcgaaa tcaagatcaa aggcggcaca ggagctcacc tcgaaggtac
   112021 ataatgatcc cggcctcgca atatggccgg ggtgagatca gattcagatt tccagaagcc
   112081 ccaggctgat ctcagccgct ctgttcaccc tcttcataat ctcctcgtcg agagtgcaga
   112141 ttcggtcatg cagacgcgtt ttgtcgattg tgcgcaactg ctccaaaagc acaacagagt
   112201 ccacggcaag gtatccctgc ccggcaggaa gttctacatg tgttggaagt ttcgcccgct
   112261 tgattcggga agtaagtgcg gcgactatcg tggtcgggct gtagtagttg ccgatatcgt
   112321 tctggatcac cagaacagga cggagccccc cttgttccga ccccaccact ggattgagat
   112381 ccgcatagaa gacatcgccc ctcttcacat ccaaagctag tcaccgcctg caaggaagcg
   112441 ttcagaaaca gcaaaggcct cgtcttcagc agcagcacat tcgcgcgcca actccagatt
   112501 gatcgaggcc atctcaatat atccatgttt cagcttctca cgcaggtcgg aacgggcatg
   112561 ctgggacagg tacgatctca ttgcctcgcg cacaacttcg ctgcggttgc ggcctgactg
   112621 cctagcacac acatccagtt cttccagaag cccggccggc atgcttacca ctatctttct
   112681 aagaagtgtc atggcctaca cccccacccg agttgtgctt cctgatatat gttcacatac
   112741 aagccaatta tatattcgtg cacatttcac tgtcaaccca aggaacagat ggaaagaagc
   112801 cttcgattac cccttctggc tggggataga tccaaacaag ggtccatgcc caacatatgc
   112861 ctgccgcccg caagctcagc aaatgagccc gcattctgcc cgtcccagcg caatgctctt
   112921 atctaggctc ctattcatcg cccgccacgt agactcttgg cacgcgcttg cttatcgcgc
   112981 aaacgatctc gtatccaata gttcccgcag ccttcgccag ctcttcggcg aatatccgat
   113041 ccccaccttg agccccgatg agcgtcactt cgtcacccac ccttacgtcc gaatcagcgg
   113101 gcaacgaaac catggtctga tccatacata ctctgccaac cacagggcat cgttccccgc
   113161 ctatcagcac ttctccgacg ttggagaggg cccgcggata gccatctgcg taacctaccg
   113221 gcaaggtagc aatgatctcc cgacctgatg ttacatacgt cgccccgtag ctcaccggtg
   113281 tgccagggcc aaccagcttg acatatgaca ccctagtatg gaaagaaagc gcaggcaccc
   113341 atggtttttc aggagcaacg gttcccaacg gcgaaagacc atacatgatg cctcctacgc
   113401 gcaccatgtc gtatgccgcc tccggcagag agagcatcgc agcgctgttt gcaacgtggc
   113461 gaatgggtat ctcatgtccc tctctgcgca acttctctgt tatgtcatca aactgctcaa
   113521 gttgccgtcc cgtcatctcc gagttacact cggcacaagc caggtgcgta aagatcccct
   113581 ctatctcgat cccaggcaac tctgccacac gcctcacaag gttggctatt tcgttcggcg
   113641 gaagcccaag cctccccatt ccggtatcga ccttcacatg tgctttggcc ttcttcccca
   113701 gggccattgc ggcctcggaa agcatctccg ccgactcgta ggacgcaatt gtggcagtca
   113761 acccgtatct tactaagtca agcgcctgcc aagggaacaa gtcctccatg aaaaggatgg
   113821 gcgcagctat ccctgccctg cgcaacgcaa ctccctcttc aaccagggcc acccccaaaa
   113881 aggagactcc ctcctcgagc gctcggatgg cgactggagc agctccatgc ccatatccgt
   113941 ctgcctttac caccgccagt atctgcgtat ccttgggtaa cgtcgatttg atttgtctca
   114001 agttccttgc aatgtttcct gtattaactt ctacccacgt tggcctcatt ccgggctgat
   114061 aaaccccttg cagcaggatt caccaccttt ttcttgtatt gggcgctcct tactgacatc
   114121 atgtcccaca gcccggcaga ttcctcaacg atatcatgat atgccttggg gatacgaagc
   114181 aatatgtcac ccgctaccat tccatgtaat ccaacttcac gcgcagcata atccccggcc
   114241 aaaccgtgca aatacacgcc ggacacagct gcccactgcg gagggacccc ctgcgccatc
   114301 aatgatccaa taactcctgc cagcacatct cctgagccag ccgttgccat gccggaattg
   114361 cccgtaaggt tcaggtaggc aatcccgtcc ggagtcgccg tcacagtccg ggcgcccttg
   114421 agtgcgacta ccgcccctga acgatgggca acgcgccgag cccactccag cctgtccgca
   114481 tttatctctt caacccctgc ctccacgagt cggctcatct cgccggggtg aggcgtaagc
   114541 acgacaggtg ccgacgcccg ctgaagcgtg tcaacccgac ctgaaagcag attgagcgca
   114601 tctgcgtcaa tcaccatggg cgttcggccc tctgctatca gagcctccac gatcagcctc
   114661 gagtcgtcag agcaagtgag ccccgggccg accactacag catcgcactt ggcagcctcg
   114721 tccaagactc tgtccacagc aattccggca atcgatcccg aactcgtttc aggcagaggc
   114781 ctcacaataa cttccgtgag ccctgcacac gcctgagctg ccacgccttc cggagcagcc
   114841 aggtaaacca aacccgctcc ggcgcgaaga gccgccattg aagctagcgc cgcagcgcca
   114901 accattcccc gggaacccgc caccaccaga acacgtccgc agtcgccttt atgagcatca
   114961 ggcctccgct gcggcaaagc tgcactcaca tcgtcagggc aaactatgca cgtcttgatt
   115021 ttgggcgaat ctgtcaacgc aggcggcaat ccgattctgt ccaccaatag ataacctacg
   115081 tgtgccgctc ctgggtacat caacaacccc gtcttgggca acccaaatgt cacagttgtg
   115141 tgagccgtta cgcaagcccc ggcaacagct ccgctccccg catctacgcc acttggaaca
   115201 tccaccgcca cgataggcac aggcgacttg ctcatcattg atatggcccg cgcaggctcc
   115261 ccgcaaggcg cgcccttagc gccagtccca agtatggcgt cgattgctac atcggctaca
   115321 gcaagatcat ccgccgccgc cgaccaagct tcttcgcaag tgagcccaac aacgtcgact
   115381 cccatattcc taagtatgtc caggtttgtg cgggcatctc ctgatatgcc gtccaaagat
   115441 ccggttacgt agactgagac gtccacacca cagcccacaa gatgcctcgc cgcaacgaag
   115501 ccatcgccgc cattgttacc ctttcccgca aatatgcaga cagagggcct tccggcactc
   115561 cagccactac gctcggctcc gatctcaact gctacccttg ctacagcggc tccggcattc
   115621 tccattagaa ctattccatc tattttgtac agctcgatag cgtccctgtc tattcttctt
   115681 gactcatctg gagtcgcaac cctcgttgca accacccccc actgaaaccg caaaagcgca
   115741 cgccatttcg tctgaatgcg aaagggatat catgaccgtg cgcgccccga tagaatccgc
   115801 acgcgcacgg gcgctcccag tcaacctcac atgaggttgt ccatattcat cctgaaaaac
   115861 ctctatatcg gtgaatgcta tcgaatccat tccacaccct agggatttca tcaccgcctc
   115921 tttcgccgcg aacctagagg cgaggtattc gggacgacca atgcgcccgt ctgagagctc
   115981 ctctgccgtg taaagccttt gggcgacccc ttgaggcgca acagcagcaa tgcgatgaaa
   116041 tcttcggatt tcgataatgt ccactcctat accgagcacg cgttggcaac acatccttga
   116101 gactcctcaa cccgccgtag cccgtctagc agagctccga ttggataatc tcggcaagag
   116161 actgcactat ccggtcgact ttagcctcgt cctttccttc acccatcacc cgaacaacgg
   116221 gttcagttcc cgaaggtcgc accaagagtc gaccactcga acccagctcg gccgcggctg
   116281 cctttaccgc ttccgctatc gtgtctgagt ggtaatagtc gtccttccgg cttacgcgga
   116341 cgttcaccat tatctgcgga tacttggtca tcacagcggc caactccgaa aggggttttc
   116401 ctgtttccac agctattcct gcgacctcta tcgctgtaat aagcccatcc ccagtcgtcg
   116461 ccttttccag gaatatgaca tgccccgatt gctctccccc aataacgagg ttcttctcga
   116521 gcatcatctg gagcacgtat ctatctcctg catcagctgt gagaacagaa cacccgatag
   116581 attccatggc atgagtaagc cccaggttgc tgtattgggt agcgacgata gcccgccccg
   116641 gaaggccccc tcgagaatgc ctgtggatgg cggatagcgc cattatctga tcgccatcta
   116701 caatgcgtcc atcagcatcg acagcgatca ccctgtccgc gtctccatca tgagcaaatc
   116761 ccatatcagc accgcgctcc actacgaccg ccgccaactc gtcaggatgc gtagatccgc
   116821 aagcgcagtt gatattgtca ccgtcgggag atgcgtttat gacgataatc tcggcgccaa
   116881 gttcacgaaa agcggctggc gtcgtcaggc atgaagcacc attagcgcaa tctaaaacga
   116941 cgcgcaatcc atccagcctg cagctggccg actcaaccgc gtgctccaca tatcgttccc
   117001 aagcatcagg cgcctctttg cgcattccca aacccgaacc cgatggtcgg ggcagtccat
   117061 cgtcctccga ccacaacaca gaacccatct ccccgctccc gcccgccaat gccctcttct
   117121 ctatgatgtc ctcgacttcg tcgggaaact tgaagccatc cgggccgaag aacttgatac
   117181 cgttgtactc ggcaggattg tgcgacgccg acaccacaat tcccgcatcc gctccgaact
   117241 tgacaactaa ataagctact gcaggcgtcg gaataacacc cacatttagt acatcaacgc
   117301 cagccgagtt tatgccagcg gacaacgcgg actcaagcat tccagtggat ctccgcgtat
   117361 cgccgccgac gattatcgtc ggtctcgccc cacttcggga caggactgac gaggcgcctg
   117421 ctctccccaa tctgaatgcc agctcaggcg tgagtcctgt attcgcaacc tccctgaccc
   117481 catctgttcc aaaaagcttg cccaatttga gccccccgtc tcttgcgcca ggccgcattc
   117541 gccggctaca agctttacca tcttgcctta gagtaaaaat cgactgtcac cgtctccgga
   117601 tagacgatca catcagacac cgtctggcca tggatatcga gagccagcac cgggacaaca
   117661 actgttgact catgtccgac agccaccatg gcaacagcac gcgcaaccct ggacaccgct
   117721 gtctctgggc cttcaatact tacttccgca ggagacacgg ataccgaaac ggacgatctt
   117781 agcccatcga ccagttccga ctctgacccc tcaagccgcc ccccatgcag accggaggca
   117841 cttccagcgg atgcgcccat ctcctcatcg gccgcctttt cgggctccgt tcctccctca
   117901 gacagtccct gtcccaatga cgccccgcct tcggcaagag attctgcttc agaacggaag
   117961 tcatcgggca ccttctgcgc aaaaaagagg tgcaacgcgc cagtgagatc gtgctcaagc
   118021 tcgacattga aatgaggcgc cgcaactatt gcaatgtcca ccgggagcga gacgacggca
   118081 cgcttctcca aagtcaagtc agccaccgct cgcgacgccc tcacaagctc gaacccgtta
   118141 ggcgccacca cctctattgg aaataggttc tggcccggct tagccccttc aaggtcaaca
   118201 acggcaacgg tagattccaa agcggtcgac tccagatctt tcggccccct gagccttata
   118261 acaacagtct caggcaaatc cgttactatc aggtcgtccg ggagatttcg caacgaaaca
   118321 ggcgctttca ccaccagttc aatctttggt gaaggcgaag tcctctgctt gtcgctgatt
   118381 gcaacagccc atattgcgat ggccaacacc acagccaaca ctttgataga cgtatcctta
   118441 gcctccggtg aaacaggttt catctgccat tgctccttag attacgtatc acagtatcct
   118501 tggtgttgtg atcgaacaaa ctttccaacc tgtcgcgaag ctcttcaggt ctcaaacccc
   118561 gaactatctc cccatcgttg gccagcgata ttgcgcctgt ctcctccgac actacaactg
   118621 ctaccgcatc agtccgctca ctaagaccaa gcgcagccct gtgcctagtg cccaacttgc
   118681 gcccagtcac atggcggtct gaaagcggca agatgcatgc cgccgccagt attcggtttc
   118741 cacgcagtat tacggcccca tcatgaagcg gagtgttagg agtaaagacg ttgatcagca
   118801 gctcagcaga tacagtgcta tcaagcttca cacctgatgc tgcaatatct tcaagtccgg
   118861 tctctccctc caagacaatg agggctccga tatactcctt cgacatggcc tccaccgccc
   118921 tagccacttc gctcagcacc tttttctttt cctctggatc gagggatttc atagggttcc
   118981 caaacagcct cccccgacct agttgctcaa gtactcttcg aaactcaggt tgaaagacaa
   119041 ttggtatgcc tacaatagcc attgtagtag cctggcgcaa cagccaatgc aatgcctcaa
   119101 gcttgagata ctccgaaatg gcatttgcag caaacagcac gacaaggcct ttgatcaaag
   119161 ataccgcctg agtgcctttg aggaagctga tgaccttgta catgagaaac gccacgatcg
   119221 ctatatccgc gattgtggtc aatgcctgca tcaaggttat gttggaaaaa tcgaaaccaa
   119281 gcaggttcat gagcaaacac tcccgaaacg ggtatcgaga ctatacatta caacatttcg
   119341 ttcttcgcgc gaatacacct tcctacataa tgcagaacct catggaagtc gagcttatct
   119401 gttcacaaaa gcgcgaatct ctccagcgcc tccactacgc catcatcatc attcgaggca
   119461 actatatagt cggcttcggc cttcacatcg tccccggcat ttcccatcgc cacaccgatg
   119521 ccggcggcac gcaacatgtc aatatcgttt tctgaatcgc caatggccat gacattatcc
   119581 atgcttatgc ccatggaaga agcaagatga gatagagcgc gccccttaga tacatccgat
   119641 ctgacaatct cgatcatgcc agggctcgac aacatagtac gcaggccagg gtcgagcacc
   119701 ccgtctatag ctttcctcac atcagcaata cgctcttcat catcgagcac caagtatttg
   119761 aaggcctcgc ggttcacgaa ttcctcgatg tcttccataa cattgatggg agcgccatat
   119821 ttctcactgt aggccactgt ctcaggagca acccgcgcaa cccataactg gtcatcgatg
   119881 taggcttgaa agactatgcc ccactcccac ataaacctta ccacacgctc gccaccgcgg
   119941 gcggcaatag gcaggcgcag gagagtgagg ccgcaattgc tgtattttat aagagcacca
   120001 ttgtaagcga ttataggcag ttcatcgagg aggccatcca ccactggctg cgatgtttgg
   120061 aacatccgcc cggtggccac ggcaaatgca acccctcgcg aatgagcctc cctaatagcc
   120121 tctatggcag catcagatat gctcttatcg gatcgaagca atgtgccatc aagatccatt
   120181 gcgaccaaac gcatcaacta acgactatcc ccccgtatca gatggttcaa ggcggagctc
   120241 acgagactga gcaacaacga agcgattatt gccgcccaaa acccgggaat attgaacgca
   120301 ttgcccataa gccaagaacc catacacagc atcagggcat tgatcaccaa agtgaaaaga
   120361 cccagactca gaatccgcag gggcagactg aacatcataa aaatcggcct tatgaaggtg
   120421 ttcacgatgc caagaaccat ggcagctatc agggcagccc ctgctccatc tatctccaag
   120481 agcgtgccgc cgcgatacgg aacgtatgag actattagca gtgcaagggc attaatgagc
   120541 cattttataa cccactctgg cattccgact tgtccattgc tgtggtaagc cattgtcaac
   120601 cctccattgc ccccatgata ggccgtaacc tgacgcctgc aaagctccgt ctcaagctat
   120661 ataggctctt caggcacgat aatccaggca atcacgtaaa gaaggagacc cggtgcctga
   120721 ctaaataacg taaaagccac ccatagcagg cgtataagag taggatcaac attcaggtag
   120781 ttggctagcc cgccacatac accgcccaac attctgtcgc gaacagacct gtatagtctc
   120841 ctctgcatta catggacacc tccattgccc ggcgtcttgc tgtattcttc agcaggtatg
   120901 caattccttc accgcacatg tcccgttccg gacacttcgc gcaagcaaac gctatccgtg
   120961 caagataccc tttacccagc agtccccgag ctcagacaac gcttatctca acctcatccc
   121021 catcttcgtc tttcacatga atcagctgcc cctcggcgcc ctcatgcacc gcatcgagaa
   121081 tggtctcaag gtcgatgttc tgatttgaga gctcattcat ggcctccctt gggataaacc
   121141 tccacatcac cttggcaagg gatatgggga tattcaagtt cacctttgag ccatcttcgt
   121201 cctttacgcg aatcctcaag aacctcgggt tccgccttga actcgcagca ggcctcgctc
   121261 gttcttcttc atatgtcatg gcgtccaaaa tctgctgcgc ctcgtcaacc gtgagtttcc
   121321 catctctcac catctcgagc acttgctttc tttcttgatc cacagagcgc acctcccctc
   121381 atatcactct catagcgcct gcaacgcggc caccgccatc acctgcaaga ctcgacagtt
   121441 atggagccgt tcatggcacg agcatcgact gtgaccttgg cactgtcatc caatgcagaa
   121501 tccccggcag aaaccgtgat cttcttgact tgcccacccg tcgaccttct ctcggaacga
   121561 atcgccgcgc cctcatcaag cactcgcgtc gccccatttt tggtttccgc attcacctcg
   121621 tacgatacat cagagctgtt caaaacctta atccgtatac tgccgttgct cgtcctgacg
   121681 ttcaaatggc cacctgattc ataggccaga gcagcagtta cgcttccatt ggaggtctcg
   121741 atgcttccat cgcccaaatg accggatgca gtaaccgctc cgttggcggt ttcagctgcg
   121801 agagtggaga ccttgatcga atcgagcgct atacgaccat tggatgaaca gacttccaca
   121861 tggcccccat tgagcctatc tagcgttatt gatccatttc gagtatgcgc aaacacatcg
   121921 tatttgcggt ccttcgggaa gtatgcctct atcgacatcg agtccagaat cgacttgtcc
   121981 tcacagtcta tgctcacttt cccgtcattg acaaccaatc ttgcaacaga ctcaagtctg
   122041 cgatctgctt cgtctctgga atccgcgtca acaactgtag ctctgactac cacacggaat
   122101 cctggttttt cccagacggc caactttata ctcccgttat tggccgcgaa atcgatggca
   122161 tttatctctg gcccgcagat ctcctcttcg aaagacttct ccccgcgcac gccgcgaaaa
   122221 ctccatccga agcctccgaa cgccgactcc accgctctgg acaaacgccc agggagttcc
   122281 tgcccagcct ctaccatttc agcgaagacc ttggataacc ggccatgcag atcgccctcg
   122341 gaaatcgttt ccacacggac cgaatcggcc tcgtctaccg attcaacctc gccttccaaa
   122401 gcagccaaca gctgctccgc ttcctccaca gtgattttac cttctgacag aagattcagg
   122461 acgatcttcc tctcttcgcc catggaccag acctccccct tttcaattag actcctactt
   122521 cccttttagt tgacgcactg catcatcagc agttatctcg cccttggcca gagactcaat
   122581 aatctctttt ctcctggtcg caacatcgtc ctcggaatca tcaaccgcat accccaatgc
   122641 cgcgatcacc gcatcaagcc ggttccggac tgtaggatac gagattccaa ggtccttctc
   122701 tacttcctta atattgcccc tgttctttat gaacacttcg acaaagttct tctgctccga
   122761 actgagctgg cagaatttgc aggtgtcgaa acggccctct atcacagtct cgcagtccgt
   122821 gcacgtcagc ctggtcacgt ccaagattcc atcgcaaaca ggacatcgtc ccaacgcctc
   122881 ccgtcttgcc ataacaacac ctcctttttg cctcccctgc ttcacattat aaagctcatc
   122941 attaatgatg tcaatgctgc gctaaataat tgtgctattc ttgcagatat tcctcggccg
   123001 tacaaacagg gtctaatatt gtagactttg ctcatccgag aacgccggtg cggttgatta
   123061 tcgtctgttc catggccaac agtgccacct tgagcggaag tcccccgcca tatcccgtga
   123121 gcctgccgcc tacgccaacc actctatgac aaggaacaac caggggaaga ggattggccc
   123181 ccacaattgc gcccgtggct ctggcagccc ttgggcggcc tgcggcggcg gcgagcgctc
   123241 cgtaggccgt aactcgccca ggagctatcc ttgtcatacc aaccatcact tcgcgggcaa
   123301 agtcgcttcc cgcagggagc tttagtgggt aatcgaacca aagcttcccc ggatccaact
   123361 cgccctcaaa gaaaagcttt atgtcgcggc acgcagctga gacaatctca gacggcggca
   123421 ctccatccca cgatacactc ccatatacag gtacgctgtt agacgcagcc atctggcaaa
   123481 tcgaggaatg atcacacatg ggaaaatcga tgcctatttg cgcaagcgta acactggcat
   123541 cgatgaacaa aggatggatc cgtgggcttg tagggaggtt tttcagggtc tccatgtcta
   123601 ttcttggaag gaccacccgg ctcaggccgc tctccccggc ctcaatcgag actatgccga
   123661 ttctcgtagc aacactggct gaagctataa tggccgaggc ctctgtagcc gatgtcccaa
   123721 tagatgaaac actgatggcg gaccgatccg gcgatgcgca cgaagaggtg gtaatgcttc
   123781 catcgtcttt ctccattcac ctgccccttt cactttttca atcatctcgt tctgtttcaa
   123841 ccaaggctca gcgctagggg cgacaatcag gagagcccga gaaaagcggc atcatagaat
   123901 tcaaccatcg actcccctgt cggggtgcaa atgctcatat acggtcatcg caaccaccct
   123961 gttcatccca ggagttgcag caatttcgtc cacagtggca tccgtgaggg cttccagcga
   124021 accgaactct ttcatcagag cgaccgttcg cttctttcct atgccgggca gatcagtcag
   124081 ttcagagctt atgctccttt ttcccctgag gttccggtgg tacgcaagag catatctgtg
   124141 ggcctcatcg cggatatact ggatcatatg gagagccgca gaatcccgcg gcaagatcaa
   124201 ggggtccggc tgctctctga ggtagatctc ctcttcgcgc tccgccagac tgataagtgg
   124261 aatgtcgccc cgtcccaggc tgtcgaggac atcagcggcc gcgttgagct gccctcgccc
   124321 cccgtctaca actatcagat cgggaaggcg tgcaaacttt gggtccgggt catcgccaat
   124381 ggctctcaca agccttctcc gtatcacctc ggccatcatg gcgtagtcat cctgcccctc
   124441 aacctgcctg atcctgaaac gcctgtactg agacttggcc ggcaagcccg cttcgaagac
   124501 aaccatcgac cccactgctt cagttccctg aagagtcgat atgtcgtagc attcaatgcg
   124561 gctcggcgac ttggaaagtc tgagatggat cgatagttca gcaagggcgt ccatgcgagc
   124621 acgggcctcc ctttgctcag cagctcgctt ccgttctaga aactccgccg cattggcaga
   124681 agcaagctcg accaatcccc gcttctcccc ccgcttggga gtcttcacag atacagcccg
   124741 cccccgaagg tcagacaacc atgcggccac tatgtctgca tcttctatct cggactccag
   124801 taaaacctca cggggaagcg accctgcctc agcctgagaa tagtattcct taacgaggga
   124861 tgacaaagtc tcagcgtcat ccgcgccgtc agttccacct agcatgaaat ggtcgttgcc
   124921 aacgagtttt ccgccacgca caaacaagat ctgtgcgcac gccccccatt gatcccgtgc
   124981 tatcgctatc acatcttggt cagccccgct ctccgagact attctctgct tctgcacgac
   125041 tgtctccacc gcctgaagct gatcacggaa aacagccgcc tgttcgtaat tgagggcatc
   125101 ggctgcggcc accatccgcc gcttgagact gcgggcaaga tcgtcctgcc gtccttcaag
   125161 aaacatcaca acctcgtcta caacttcacc gtatgcttca acgtcgatct gcccggcaca
   125221 tggaccaagg cacctcttga tctgataatt taggcaagga cgcggtgcgg gagaatccgg
   125281 gtcaatcttc tttgagcact gtcgtagcgg aaagaccctt ttcagaaggc ggagggtttc
   125341 gcgcgtcgca gagacatccg tgtaagggcc gaaataacga gacccgtcac gcacaaccct
   125401 cctcgtcaag tatacgccag ggtagggctc ccccgtcgtc accttgatga aaggatacgt
   125461 cttatcgtca cggagcctaa cattgtatct tggtctgtat ttcttgatca ggttacactc
   125521 gaggatcagt gcttccagtt cggacgctgt cacaatgtaa tccaaatcgg ctatgtgctc
   125581 caccatcatg acaacctttg gcgaatggtc agaagaagtc tgaaagtatg atctcaccct
   125641 gtttctgagg ttgatggctt tgccgacgta gattacctcc ccgtcggcat ccttcatcag
   125701 atacacgccc ggactcgtgg gaagaacagc caaggtatca tctagtgcca cctgtcatcg
   125761 cctccagctc gcgttgggca ggcattactc ccgctatctt caccaaatac tcccccgtgt
   125821 acgaccccgg ggtcgccgcc acctcctcgg gcgtgccggc ggcgatcacc cgtccgccct
   125881 tgtcgccgcc ttcgggcccc agatctatga tgtagtctgc agtttttatc acatcgatat
   125941 tgtgttcaat cactacgaca gtatcgcctc catccacgag acgctgcaga acatcgagga
   126001 gcttctgcac atcggcaaag tgcaagcccg tcgtgggctc gtcaagcacg tagagggtcc
   126061 ttccattggt gcgcctcgac agttctgtgg cgagtttaac ccgctgagcc tcgccccccg
   126121 aaagcgtggt tgccggttgg cctagcttga tgtagccaag gcctacatcg tagatggttt
   126181 gcaactttct ctttatcgca ggaatgttcg caaagaactc caaagcctcc tcaacagtca
   126241 tttcgagcac gtcagatatg ctctttgact tgtattttac ctccagagtc tcccgattgt
   126301 agcgtgctcc gtggcagacc tcgcatggca cgtagacatc gggcaaaaaa tgcatttcta
   126361 tcttgatgat cccatctcca cgacacgctt cacaccttcc gcccttgacg ttgaagctga
   126421 aacgaccggg tttgtatccc ctagccctag cctcaggcgt cttggcaaag acctccctga
   126481 tatgcgtaaa aactccggta taggttgccg ggttcgatcg gggcgtccga ccgatgggag
   126541 actgatcgat gcctatgacc ttgtcgagat gctcgatgcc gagaatctca tcatgcgcgc
   126601 ccggtctggt cattgcgcga tgaaggtcat gtgccaaacg cgcatagagt atctcgttga
   126661 tcagtgtgct cttgcccgaa cccgagactc ccgtaacgca tacgaaaacc cccatgggta
   126721 tagtgacatc aatattcttc agattatgtt cgcgagcgcc cttaatgaca agcgcgtttc
   126781 cgttaggcaa tcgtctggct gccggaactt caatacgcct tacgcccgac agatactggc
   126841 ctgttattga tgcgggatta gcctctatct cttcaaccgt gcccgcggcc accacttggc
   126901 ccccgtgtgc gccggccccg ggccccatgt cgacgatata gtcggccgac cgtatcatct
   126961 cctcatcgtg ttcaacaact atgacggtat tgcccaggtc acgtaggtct ttcaaggtat
   127021 cgataagacg agcattgtcg cgctggtgaa gcccgataga cggctcgtcc aggatataga
   127081 gaaccccgac caagctcgag ccgatctggg tagccagcct gatccgctgg gactcaccgc
   127141 cagacaaagt cccagcctcg cggtggaggg taagatagtc gaggcctaca ttggtaagaa
   127201 acccgagcct tgcaccgatc tccttgagaa tctgccttgc aatcagcgac tccctctctg
   127261 taagccctct tccgagttcc tcaaagaact cgagcgcctt cacagtggac atcgacgtga
   127321 cctcggcaat ggattttcct cctacagtca ctgcgagcgc ctccggtttg agcctggcac
   127381 cattgcactt tgtacatggg ctcgcgctca tgaaccgctc caaattttcc cttatgtagt
   127441 cagacgtcgt ctccttgtag cgcctctgaa ggttgggtat gaccccttca aacctcgatt
   127501 cccagtattt agtgcgccct cgtgagttaa catagcgaaa cctaaatttc tcgctcccgc
   127561 acccatacat gatcatctcc attgcgctct ctggaagctc cgacatggga accgacgtat
   127621 cgatcccctt ctcggtagcc acctggttaa ccctgcccat gtagtaatcg gctattacgc
   127681 cgtttccgtt gtatggaaga atagcgccgt cttcaatcga gtgttccatg tcgaagatga
   127741 gtttgggatc gaaatctatc ttcactccta acccggaaca gacaggacat gcgccgtatg
   127801 ggctattgaa tgagaagagc ctcggctcca gttcaccaaa gcctatcccg caatcagggc
   127861 acgccaactt ctcagagaag accagctctt ttccgcccac aacatcgatg atgacgaccc
   127921 cgtctccaag cttgagcgcg gtttcgagcg aatccgcaag cctagtctcg atccctggcc
   127981 ttaccgccag cctgtccaca acagcttcga tgctatgcat cttgtatcgt tcaagagaga
   128041 tctcctcgga aagctccatc acctcgccgt ccacacgcac acgaatgtac ccgtctcgct
   128101 tgaggtcgtc aagggccttc cgatgctcgc cttttttgcc cctgataaca gggccaagca
   128161 cctgtatccg cgagccctcc ggcatagcca tgatcctgtc gacaatctgc tggacagtct
   128221 gttgcgctat ttccctgccg caattggggc aatggggaat ccctatccta gcgtaaagaa
   128281 gcctcatgta atcgtaaatc tctgtaacag ttgccaccgt cgaacgcggg tttcggccct
   128341 gcgccttctg atcaatggag atagcgggcg aaagcccttc gatgtagtct agggcgggct
   128401 tgttcatctg tcctaagaac tgtctggcgt aagcggacag agactccaca taccgccgtt
   128461 gcccttcggc atatatggtg tcaaaagcca acgatgactt gccagagcct gacaaccccg
   128521 taacgaccac aagacgatct cgcggcaagt caagatcgat gttctttaag ttgtgctccc
   128581 gcgctcccct gataattatc ttctctacgc tcatagctcg acgaacctcc gtctgagatc
   128641 acgaatagtg tctcttaact cggcagcgcg ttcaaactcg agaagacccg ccgccttctt
   128701 catctcatcc tcaagtgcct taatgtatcg agcgagctct cgctcatcca tggattccac
   128761 atctgcacag ccgaactcag cggcaactcc accctgtcct ctgaccccag attgcccgcc
   128821 acaggctgca ctgctgccaa cggatctgtc tgcgacccgc tgaatctcgg atagatcgcg
   128881 cacctctttt ttgatagatt caggagttat cccgtgctct ctgttgaatt ggatctgcgc
   128941 tgaacggcgc cgttcagtct cgtcgatagc ctcgcgcata gacccggtta tctggtcagc
   129001 atacattatt acacgtccat ctacgttgcg agcagcacgt ccaatagtct gtatcagcga
   129061 tgtatctgac ctcaaaaacc cttccttgtc ggcatcgagt atggcaacga ggcctacctc
   129121 cggaagatcc aacccctccc tgagtaagtt gatcccaacc agcacatcga agtcgcccag
   129181 gcgaagacct cgcaggatct ccacgcgttc caatgtctgt atctcggaat ggaggtatct
   129241 caccttgata cctatctctc tgaggtaatc tgtgagatcc tcggccatcc gctttgtaag
   129301 ggtggtaacg agaaccctgt atccagcgtc cacagtgcgc ctgatttcac cgatcaaatc
   129361 atcgacctgg ccccgcacag gcttcaccgt gaccgccgga tccacaagcc ccgtcggacg
   129421 aatgatctgc tccaccactt ggctgcttac cgtcctttca taatcagccg gcgtagccga
   129481 gacgaatatg acctggttca ccctctcctc gaactcgcta aacgtgaggg gcctgttgtc
   129541 gaaagcagat ggaagcctga atccgtactg gaccaagctt tccttgcgcg aacgatcgcc
   129601 attgtacatc gcccgtatct ggggcacagt gacgtgcgat tcatcgatga acatgaggta
   129661 gtcatcggga aaatagtcca gcagcgtcgc aggagcactg cccgcttccc tgccggcaag
   129721 atggcgcgag tagttttcta tcccggagca atagcccact tcctccagca tttcaatgtc
   129781 gaacatggtc cgctgctcca acctctgggc ttccaggagt tttccatcac gcttgagctc
   129841 cttgagcctc tccgcgagtt caaccccgat ggcctcaatc gccgcatcaa gcctctcctt
   129901 gcttgtaacg tagtgagtgg cagggtagat gaaagccgat tccctcagtc caagcacctc
   129961 gccggtaatg aggtcaatgt cggaaatgcg atcaacctcg tcgccgaaga attctatgcg
   130021 tatcgcttct tccccgcttg caggtatgat ctccacaaca tcgcccctga cgcggaaagt
   130081 gccacgggta atgccaatat cattgcggct gtactggatc cccactaact tgcggagcag
   130141 cctatctcgg tccaagtatt cgcctgcatt taccacgact gcctgattga ggtattcctc
   130201 gggcatgccc aatccataga tgcacgaaac actcgctacg attattacgt cacgcctctc
   130261 aaccagagcg gcagtagcag catggcgcat tcgatcaatc tcgtcattta tctgagcatc
   130321 cttctcgatg tatgtgtcag actgaggtat ataggcctca ggctggtaat aatcgtagta
   130381 gctcacaaag taatgaacag cgttgttggg gaaaaactcc ctgaactccg acgccaattg
   130441 tgcagcaaga gtcttgttgt gagcaatgac caacgtaggt cggcccagtt cctggatgac
   130501 attggccatc gtgaacgtct tgccacttcc cgtgactcca agcaggacct gcccgcgcat
   130561 gcccgccttc actcccgcaa cgagctgcgc gatagccctc ggctgatcgc ctgcaggggc
   130621 aaaacccgat ttcagctgga attggggcat tgcagaccac tcctttcagc ggatgacggt
   130681 tcaattatat cacgccccga acatacattc tatccgcgat aacagtttaa tgcgccaaac
   130741 aatcatccgg aagcatctaa agctcacatt gccaggattc ccccacttcc catgctcctc
   130801 aataagccct tcagctcgtc accacgcaat tcttccctgt tcccctgcaa aagccgagat
   130861 tatggctcca ccgccttgcc atcaatacgg ctgcagcacg cgaaccttga gcacaatcac
   130921 ggcacgttct cccatacacc ctaaacggca gaaacgcgcc ccaacaaaac tgtagcggcc
   130981 cctcggggcc gcattccttc ttggtccatt tatttgtgtg actttagatc tggacacaac
   131041 cagccgaagg ccggttaagg ctctgatagg tcaaccccgg gatcatctac agtctgaccc
   131101 gtattcgtct cgccacactc cccaggctcg ggttctagct cctgcctggg ctcaggctca
   131161 gaaaaggctt gaggttcgac tgctggaaga gcctcaatac cagcttcctc cttgtcggct
   131221 tcaacaggca actggacagg cgctgcggga gccgcttcct tgacggtggc tggcactgcg
   131281 ggcagactcg gtttcggttc tgccgttcgg ctcggcgtcg gcatcagctt atcgagagtc
   131341 actagttcaa ccccggcagc atccaactcg ggaagcatct tctgtattgc acgtactgtg
   131401 gcaggacgca catggcctat tgcggcagcg gaacctcgct ccattgccaa cctcgctact
   131461 atccttatgg attcgataac atactcctcg gtatctatgt gatctaaaaa cctagagttt
   131521 tcaaggagcg gaagccccat ctcacgagcc acccgcggaa ccaccgagtt cgagctcgtc
   131581 cggctgtcga aaaagaacaa gccatgagca aaaacctcat cgagtataat cctgacactt
   131641 tccgggtctt cagtgacacg cgaacccatg tggttattga ctccaatagc attgggcacc
   131701 aaagctaaat tggacgatat gacgctccgt atctccttct catccatacc catcagtatc
   131761 ccgcccttgc cggggcgctt tgacgggtcc aatggctcca taggctggtg tagtaggact
   131821 gcaaagcctc gttcggaagc ggccttggcc tccttcgcag catattttcc ctgtggaatg
   131881 acggccacag taagcggcac atctatactc aaaagctcat ctgtagcagc aactcccgat
   131941 ccaaggtcat ctatgactat cgcaagccga ggcctgctgc cgttaagagt cttcctctcg
   132001 gaggatgaca gtggcggttc cttcgatgtg tatctgggga caacgttttc ttgcggcttg
   132061 gagagaactg aacccgctcc aactggcatt tcctccaaat caagcaaggc ttcgtcgcga
   132121 cctgacgcgg tctcttcaat gctgaaggtt tccgaaacgt ccaggccttg aatggattcc
   132181 aagtattgct cgttccgcgt cggcgccaca gtctgcccca ggctccggct ccacggaacc
   132241 acaacttgca ttatctcgag atacgtggca aggccctgca ggcttcgatc gctgaagccc
   132301 aatatgaaaa tggcgcaatc gcctgcagag gaatctccat cggcaatctc ctgaataact
   132361 ctggcattgg ccttacgcgc cgcacggctt acagctgccg acaaagactg atagctagca
   132421 gatgaagcgc ccgcttcgta cgaggtgatt gtccagcgaa gataggtatc ttctttggac
   132481 gaaagccgca taatcttttc ctgcctgcca tttgcggcca cacttttgtc gatcactcca
   132541 gattccagaa gcgctttggt tagctcagat gaaagcttct ccgatttgcg tgaatagtca
   132601 tatatcgtct ggccatcttc atgaaccacc tttacctctt tgccacatcc tgtcagcgtc
   132661 atggcgaggc atgccaagac cgccacccac aagctgtatg gcttcctttt catctcctgc
   132721 gcctccgtcg actgctgcgt cgttttatgc gatccgcgat gatcgcgatc agtcctgcac
   132781 ggccagagtc catcttcggg tagtcccctt ccgtaggcac gaggagcaga cctagcccat
   132841 ccggaactcc tctgatctga acagtcctgt tttctccttt actgccgggc aagccagatc
   132901 gaatatcaag gattgtggca taccccacac tgcgtagcgc ctcagcaaag tcggcaaatg
   132961 agttgaccgg ctggcaattc acagcttcga tcacatcccc gggcttgata cccgcctggt
   133021 cgcagggaga ccccggcccc acatcgagaa ctctgatgcc ttgcgcaggc gttgtgaaca
   133081 atggctctcc ttcgagctcg gctttttgcg acataccaat gacgaactcg tgcccaatag
   133141 gcgagaacag tgctgctacc catctgaccc acggatacct ttcagataaa agagtgaggc
   133201 caagaagcgc caagctgaat agggccgaca tgccggcagt cgctttcgtc ttctgccttg
   133261 gcaaacggct gtatgtcgca tccccatatc ccatggcaac cgtaatggcc aagacccagt
   133321 agtcagcgcg catgtcagga gatgggggtg cagcggactt aagaagcggc caccaatctg
   133381 gcatggcgat tccgccttcc ggggttcctg ttgtagcgag actgtaagtt atccataaaa
   133441 tcgcaatggg aacaggccaa tacttgctca tgtagaaacc gcctgcaggc ttgttatctg
   133501 ggcctgatac tatgacagga gcagcgtcgc gatgcccgct gagcaatatg agcgcggatt
   133561 ccacaagatg gagcaccccg actaaagcca tgattgatgt gacatccaca tccggccacc
   133621 caaaggccag gtttagaagg gacactatgc cgccggcata cgagaaacag atgaacctga
   133681 tatctatcga tgccagcgcc aatgctatta accaaatcgc cccaagaccc aaactttccg
   133741 ctgaagcgcc aaacaggatt atcagaaaac tgccgagtat tcccccaacc atgccctctc
   133801 ctagggagca catggttcgc actatcgcac tccgcctcgg tctgccaaat atctgcgttt
   133861 ccaaggccga cattcgccga tactggaagt atactatgct cattacaatc caaaaccata
   133921 tcatactgga gggatagcga aacaacgcta tctggctctc cagtatgatt ctgaacacgt
   133981 tgccaaatgg gaattcctgc aactgcaaca ctccgctcta cgcggcgctc ttgacgggct
   134041 catccttctc aatctggtat cttaggatct cgagagcacg tcggaactgt ggatccaaat
   134101 caggatctat ccgcatcaaa tcttctgcgg taacagggcc cgtgcgctcg gcattccggg
   134161 cctgatcatt catggctttc tcaagctctt cgggcgtcaa cgcctccact ttcacatccg
   134221 gcattattgg ggtttcaggc gtgatctccc gtcctcccgc cgtaaggtat atctgcgtag
   134281 ttaccgcaag ccccgaacca tcagaaagag ggaatagagt ctgaacgagc ccttttccaa
   134341 acgtcggcac tcccactatg gtggctacgc ccgtatcctg caatgctccg gaaacgatct
   134401 ctgaggcaga tgccgtgtac tcattgacca aaacgaccaa cgggatctgg gaaacacgtc
   134461 tgccaacggt gtaaaggctt ttcttctgac catcgcgtcc cctaacatgc atcaccggtc
   134521 cgggcggcat gaacttgctt gcaacctcta cagcttgtgt tacaaggcct ccgccgttat
   134581 atcgcaggtc aaggatgagg ccgcgcatgc cctcatccat gagcctttca acttcctctt
   134641 cgatatcctg tcctgccttc tgcgagaagg ttcgaagctc gatataacct atgcccgcat
   134701 cggggtcaaa gatgtagcca cgggtagcag gcgcgtctat gatgtcgcgc actatttcca
   134761 cttcgaactt ttcggccgga tatctgcgtt caatgaggag gcgaacaaca gtcccaggct
   134821 cgccgcgcat catcgataca gcgcggtcca gaaccatgcc ttctgtagaa actccatcaa
   134881 tttccacgat gtgatcccca ggcatcagtc ctgcacgcat tcccggcgta ttcgtgatag
   134941 gactgattat ggtgatctta tcatccctta tgccaacaag gatcccgatt ccgccgaata
   135001 tccctgcaga atcgtctttg agggcctgga agtcttgggg tgtcatatac ctggtatagg
   135061 ggtcattgag cacttccagc attccgggga cggttccttt tgcgaagtat gccttcatca
   135121 gctcagctac tgaaactggc ttatagaact gagccttgac aagatacatc gcttcaatgg
   135181 cggtgtgaat atctttgaat ttaggcgcag cctcttcatc ctgtggcgaa ggcacgccaa
   135241 atgctgatgg agccaacacc gtgcacacaa gtattgctgc tgctacggta agtacaagcc
   135301 aagcagatct ctgcgctttc ttatatgaag acatgattcg gcacccactt tcacaagtcc
   135361 atacatccta tgctaaaggc tctgagaatc gaaccgccaa gagggcgtcg atcttcaggg
   135421 cagccaacgc atgggatctt gaggggtccc gtcttgcctg acttcaaaat gcagatgagg
   135481 tccggtcgaa acgcctgtcg atcccacttt gcatataata tcaccctgca gcaccacatt
   135541 gccagctttt gtcgtgataa cgctggcatg gccatataac gtagaaaggc cgtttccgtg
   135601 gtcaaggata acggtgttgc cgtatccatt tacccatccc gccaaaagga ccttgccgtc
   135661 agcagcagct ttaacaggag tgccggtgct tgcggccaag tccatgcctg agtggaaccg
   135721 tctggtctta tatatgggat ggactcgcca gccaaacaca tctgatattc tcgtgaactt
   135781 aacaggccag ataaacttgc ccgtccagct gattgtcccg cccgtcctag cctgcatctc
   135841 cctgatgatc cgttccagct ccctcgatgc acgctcttcc tcagccagct cccgttccca
   135901 tctctcacgt tctgccgata ccctgctggc gtattgcgat cgttgagcca atacgggctc
   135961 gatggacctt cgctcgtcct ccaaggcgga cgacaggagg gctatctctc tcctggtctc
   136021 ctcgagtttg gcctttcggg cacagatgcc ttccttctgc tcctcaagag attccttttc
   136081 ggccctgatc tcgccatata tgtccgcgtc ctgcttcgct attgccccga gataatcgta
   136141 tctgctcaca aaatcagaga agctcgatgc gccaaaaagc aactccagca atgttcccgg
   136201 accaagcttg taaatgaata cgagcctatc ttcgagactt tgaaccgagg cttcatactg
   136261 cgctgttgcg atctcaagct ggcgctcggc ttccttcaac tcagtctgaa cctgaaccaa
   136321 ctgctgctcc gcatctttga gcttcttttc ggtctgagaa atcctcgttt cgatcgcaag
   136381 gatctcttct tctgttttct ggagggcccg cagtgcgtcc ttctcttcct tctgcttgcg
   136441 cgacaatgtc tgtcttatct gctctatctt agattgcaag ctgttgagtt cggttctctt
   136501 gctggaaata tccgactgtt gcgagctagc aacagcaaca tctgtaagga acccaaaagc
   136561 aagcgtcata atgagaacca aggaaaggac tgccctcgca gctttaatga tgcccacttt
   136621 tcttgcctct ggcgtcatag gaggaacccc cgtatcctaa actttaaggt atcgcttcgt
   136681 cgaaatatta cttcccacaa gaccgacagc agccccaaag caaagcatta tcacgatggc
   136741 taaaccgaga tggccgctat cagtaacgat cggcatgaac ggcgccacag aagagatcca
   136801 cttggccaag ccctggtacc cagcgcccac aatcaccgca gcggcgccgg cgcccaaaag
   136861 gcctatgatt atcccctcaa gcacgaaggg gaaaagtatg aacgcgtcag ttgctccaac
   136921 caacttcatg atctctatct ccttgcgccg cgcaaatacc gtcagcttta tcgtgttgga
   136981 gacaatcaga ctggccccga ccaaaacgaa gacagctatt atccctgtca caacccagaa
   137041 tattcgaaga acgcccacaa gtcggtctac gagttttggg gcatacctca catcggaaac
   137101 ttcatccagt tcgagcacgg cagcggcaat cctatccgcg tccgcagcgc gcaacagaga
   137161 tatgtcgaag gattcgggca gcgggttgcg gggaagatcg ccaagaaaga agttgttctc
   137221 gcctagctgt ttccccagcc tctgcagagc ctcgtccttt cccacgtatt tcaccgacgc
   137281 tactcccgga atagcctgta tggcggcgtg cgtccggtca acagcatcgg gcggcatatc
   137341 cggcgacaaa aaagccctta tctgtacgcc agcctgaaac tcgtttccta tgcgcgtcag
   137401 attgaccgca accagcatga aagagctgag gaccagcatg catgccatta ccacgataat
   137461 cgatgtcaca gatggtgtct tcttcaggct gcggaaagcc tggccgctga gggacttcac
   137521 cgtcctaagc ttcatggcag taagctcccc acctctcgtc gcgcacgatc atgccacgtt
   137581 ccaactgaac tactcgcttt ctcatcttgt ccaccactac atggttgtgc gtggccatca
   137641 ctacagtagt gccccgcaca ttgatgtcac ataaaagatc cattatgtcc aatgtagtat
   137701 ccgggtcaag gttccccgtg ggttcatcgg ctacgagtat gagggggtcg ttgacaatcg
   137761 ccctcgctat ggccacacgt tgctgttcgc cgccggcaag ctcttcgggc atggccgaag
   137821 ccttgtgggc aaggcccacc atggaaaggg cctgcggcac cttcttctcg atctccttcg
   137881 gagacgcctc tatgacttcg agggcaaaag ccacgttatc atagacggag cgatcaggca
   137941 gcaacttgta gtcctgaaag ataaccccga tccgacgccg gagcatggga acgtcgcgcc
   138001 acctgagccg ggcgacgttg cgcccggcaa ccaggacgtg cccgcgcgta ggcaccgtcc
   138061 cccggtatat caacgaaagg agcgtggact ttcccgagcc actagggccc acaatgaaca
   138121 cgaaatcccc tttgtctata ttaaggttta ccccgcgcag cgcttccacg ttgcccggaa
   138181 acaccttggt aaccccgtac atattgacca aggtcaacca ccccatcttc agctggctat
   138241 aacagcatac cgtatatcaa aaacaggttc tccttaagag gcgcaaattc ctgctgaagc
   138301 gaagcggaaa acatgacgct tccggccaac atctgctttt ataatgtatt tctacgccct
   138361 tttcatctct atggcgcgct ttgcagcaaa tgatatgttc tcaacagtaa gcccataagc
   138421 ttccagaagt tcgtcgggct tgccggactt tccgaacata tccgctacgc ccacgcgtat
   138481 cactggcgcc ggagcacgtg aacacacggt ttcgcatacc gcgccaccaa ggcctcctat
   138541 gactgagtgc tcttccactg tgacgattgc gccggtttcg accgccgccg cacgcacggc
   138601 ctcttcgtcc aagggcttta tggtatgcat gtcaagcacg cgcgcccgaa tccctctttc
   138661 ctcgagcctg tcggcggctt cgagggcccg cgataccatg accccgcacg caattatcgt
   138721 tacatcttgc ccttccctca gcgtcttcgc cttgccaagc tcgaaatcgg cgtcttcatc
   138781 gtaaagcgtc ggcacggaag cccggcccag tctgaggtag aacggtccct tctgttccgc
   138841 ggcaatacga actgcccacc cagtctggac tgcatcagaa ggcactatca cagtcatgtt
   138901 gggaaggact ctcatcaacg cgatgtcctc gatagactgg tgagaccctc catcctctcc
   138961 cactgtaagc ccggcatggg tcgcgcatat cttcacgttc agtccaggcg cggcgatgct
   139021 ctggcgcacc tgatcgaaag cgcgcccagt ggcaaatact gcaaacgtgc ttgcaaacgc
   139081 tgtcttgccc gtggcagcga gacctgcagc agtgcccatc atgtcggcct cagcaattcc
   139141 catctgtatg aagcgctccg ggtactttga ggcaaacccg gcagtcttgg tcgatccaga
   139201 aaggtcggca tcgagcacta cgatgtccga gttgatcccg cccaactcgg caagggcctc
   139261 tccataggcc actctggtag ctatcatctt tgtcattgcg ccacaccgcc ttttcgtgat
   139321 gcttcaagtt ccgccaatgc cgcctctacc tgttcgggtt tgggagcagt accgtgccat
   139381 cctgcttcgt tctccataaa ggagactcct ttgcccttaa cggttcgagc gactatcgcc
   139441 gtaggcctcc cgctggttcg ctgggcctcg tctatagccg ccattatcgc gggtatgtcg
   139501 tggccgtcta tctctattac atgccatcca aacgcggcaa accgttcacc tatatggtca
   139561 acagacttca cttcgcggat aggcccgtca atctgaagtc cattccagtc cacgaatata
   139621 gtcaggttgt ccagcttcct gtggacagcg gtcatggcgg cctcccacac ctgaccctct
   139681 tcgatttcgc catcacctaa gacggcatag acgcgccaat cagcaccatc catcttcgcg
   139741 gcaatcgcca tgccgcacgc tgcagagagc ccctgtccga gggatcctgt ggacatgtcc
   139801 acgcccggcg tctgattcat gtcgggatgc ccctggagat aactgtcgat ccttctcaaa
   139861 gtaatcagat catccaccgg aaagtaacct gcttcagcca gtgcggcata aagcacggga
   139921 gcggcatgtc ccttcgacaa cacgaatcta tcgcggccag accatgtcgg atcatccgga
   139981 cgaatcctca tcttgttgaa gtaaagcaca gtcacaatgt cagcagccga caatgagcct
   140041 ccagggtgac cagatgatgc ggcgccaacc atgcgaatta catgcttgcg gatctcgcac
   140101 gccttagcgg cgagttcctc tgggtctgag agcacgactc ttttcaacgt catcaactcc
   140161 ttctgctctt gcgaaaagcc tcacccatga ctgcaaagac gaatgcgggt aaagccgtca
   140221 tcctctttgc tctgcgaggt tctgatacta gcctatataa ccactccaag cccaacttct
   140281 gaaaggcagc gggcgcccgc ttcacacttc cggacatcac atcgaaggag cctccgactc
   140341 ctatccatac cgcgcatggc gccatctggc gcgccttcgt tatccatttt tcctgctttc
   140401 ccgctcccat tcccacaaag acgaagttag gggcggcatc gcgaatacag cggagaactt
   140461 cctcctcctc ggcatctgag aaataaccgt gccaagttcc tgcaatcttg aggccaggga
   140521 attccttgct catgttctct gcagctgcct cggcgatgcc aggtgccgcg cctacaagga
   140581 agaatgacat ttcgcctcca gccgccgagc gcatcaaatt cgtcgccaac tctatcccag
   140641 gcaccctccc cgggggcttg tatccgagga ttctcagccc gcgcaccaca cccacgccat
   140701 ctggaacaac caagtcagcc ccgcggataa catcagcgag ttcagcatcg cgcttggctg
   140761 caagcgccat ctcggggttt atcgtcacca catgggctcc caagcctaaa gcagcacgct
   140821 ccatcaatat gtatactgca tcatccatgc caatacagtc caccggcgat ccaagaactg
   140881 acgccctcat atagtctttg ccttcctccg cttgggcttt cattctttct gtcacgggct
   140941 tgtccaatat aggggaaagc gagatgctcg cagccgcctt cgcctgctct cttatggtcg
   141001 gcataatctc cctcaacctc gccctcgttt catccctaga tgcccacgca ccggcaatcg
   141061 ccgcgtagag ttgatcagct gacgccccca cttccactat gtacggttcc gacacgcgtt
   141121 ggcagtgcgc ccgaatctta ggatcgtagt ctattgcgac agcgggcacc cctgcatttg
   141181 cggcaaatat cagagagtgc agcctcattc ccaccaccat ctcagctcgg gatatcaacc
   141241 caagcacttc ctccggcacc atgtcacacg agataacagc aggacttttt tcgccggccc
   141301 tacattgatc tgcaattcgg tgagccacct caagatcgcg gtcaggctgg aacgccagga
   141361 aaatagggca tatgcccagc tccgatactg cacggtcgat ggctaacgca tattcagcct
   141421 cgcgaccatc aagccccggc catggcctca aacagaacat caccgtattc ggtgcttgaa
   141481 cagccatggc gtccggcggg tcgcagggaa caagcccaaa agcgggatcg gcaactagca
   141541 tgacctcagg cttcacaacg ccgacctcct gcagcagacg ggcagaggcc tcatcccgca
   141601 cagttatgac ggaggcccga ggaagcgtgc gcttcaccag ccatctcgaa aaagcacgat
   141661 tcaaagggcc aactccctgt gcgtacactg aaaccggccg acccatcatc cgcgcaaggg
   141721 caatgatgcc aagatagtat gggaccgatt tcttgcctgt tgcgtcctgc aagaggctgc
   141781 ccccgccgct tatgacgatg tcagaccggc gaatctcgcg aatgatagct ccaagcgacg
   141841 ccctaggcac cgcctctatg ccgtgtgccg cgctagtctc tgatggatcg ccagagagcg
   141901 ccacacactc tgccttcgga tctatttcct tgaggtgttg gacaaatgcg gcaagcaccg
   141961 cctcgtctcc gagattcccc attccatggt agccggagac caagacccgc atactgcact
   142021 cacctgcttt cccacgcacg acacttactc aagatcatcc ttcccggccc gcagcccgac
   142081 gtatctgggc aactacggcc ctatagacca cggttgcaac aagaccgatc gcaaacccga
   142141 gccccagtcc gataatgacc ctggccaatg tgaggcttat gggagtatgg atatgagcga
   142201 acgtgttcgt cagcgaaatc tggcctatga ggcccaaaat ggagatgggc aacagtagac
   142261 cagtctcccc caaagcaaca agagcgccag caagaacaaa agccgggtgc ccgatgagga
   142321 attccttagt tcggggcctt gcggggaaga tgttctccaa agcagccctc attttctgct
   142381 cccattgggg aaccgctacc ggaacagacg atgtattgcc agttctcgca atgtatatca
   142441 gccctgcaac acccagaatc gcaatgacaa tcacgtgcca cacgaggata ttggatctgt
   142501 tgagcctagc caatgaggct atgagcgatt cattgccccg tctcgcatac cgcttccaat
   142561 aagccactaa cacgaacaac attgggaaga tgtgcattgc ctttaccccc aaaaactgtt
   142621 cgcctttgac catgaacgcg cggctcgtaa ggcatgcggc aagcatgagc ccaccggcaa
   142681 ggcatagcat cgatgcggta aggaatttgg ttcctgaaga tcccagccag tctttgtttg
   142741 ccaagagcgc cccatcgtcg caagtatcgc aatccctggt tttcccggga atatcggggc
   142801 agaacagaac aaaagcagcg agcgagggaa acgcaatggc tgcagcgagc gcaacagatt
   142861 gcctcgccaa gagcggatat cccaatcccc aaagagcagc aaatccagcg gcaaacaccg
   142921 caacaagccc tatgcatatg gcaaacggca gatcccaaat ggcatcaagc aacactacac
   142981 atccagccgc aacacccaag actaccagaa gcgtcagaag aagcccagga tcaagttgat
   143041 cgaatggtgc agccgggcca agcgaaaagc cttggtccgt gattgcgcta accacttctg
   143101 ccaccaaaga ctggttaacc tcggggagac tgcgttcgtc aatgggcttc atcataggac
   143161 gcatgtagag cactcgtatg ttgcgctccc ggacagccct ggcatacctg tctctcatcg
   143221 cagctacaga aagatcgcga tcgacttccg ccctgctaat gctgtggact cgcactgtct
   143281 tgtaatcgag agcccgggcc agctggcgtc cgcctgcttg attctggaat tctatgtcgc
   143341 cataaactgt tcccaagcga tccagtccgc acactgtagc ggatacaaat cgcggataac
   143401 ccaggacttc agttccagcg aatatgacgc aatccggaat agcaccgaca gctgacaatg
   143461 tcatcacaga gtctatccac ttttccgaca cattggggaa gttggaaaat ctaacgacca
   143521 cacggagccc ggcagcctgc acttgcgccg tcttcgacgg gcggattccc agattgagct
   143581 tgcgtgcctc atcgacagga agagagactg cgtatcccgt tgcatctccc gccctgatca
   143641 cggagatgga aggcccatcg cacgtacgca aagtcaagcg gttcgcaaga aaagttgcgg
   143701 cgtccacatt gcacgggatg acaatagtat ccgaaggtga aaagacctcc atctctgcaa
   143761 ccgccaacag gatatccctg tacgggcttc cgttcgatcc cagtgcaagc gcctcagagc
   143821 ccctgatgat cacgatctcc ccagccgtct ccatcgagcc tagggtatcc tcctccaagg
   143881 cgaccgagac aataccctcc tcagcgaggc ctcgcaggca gtcctcgata ttgatgccaa
   143941 cgcggcgact gtaaagagcg atttcgctgt aatccatgca aagctcaaca acactgttag
   144001 ccttctcaac acgatttctt tgccaggcta tccaagcaga tgatactgaa gccaacacga
   144061 tcagcgcaat cagccaccca atagaccgag gtttcctgtg gcccatcaca gactgcctcc
   144121 ctccgaagtg cgacccagca gacggaccat gccttcgccc acttcaacgc tttctaactc
   144181 tatgtcaaag gggagatccg aaaggtcgat cccaatagct atcccatctt cgaacatgtc
   144241 cacaataaaa gctggaactt cactcttctc cacaatgagc ttgtcaacta cgtagactag
   144301 tttgactccg tctcctatgc taaaatgtcc ctgcgattca acgcccacac tgaagccgcc
   144361 aaggaaaagc cgtcccgcca attctgcgat tccggggtgg aaccgaacac cgaaggcgcg
   144421 aaggtaatcg tccctgtgac ctaagtagtc attgacatcg ctctcatcta taatgacctc
   144481 cagctgggaa ttgccgagag cacgagccca gcggctagat cccggttcga atcttcccag
   144541 agggattcga gcatcggacg agtcaagcga aattcgcctg atcacaaagc ccgaggacac
   144601 agcttcttca atatcaattg aaagcctgtc aaagtgtcca tataagagtt tcagagcagg
   144661 catcgctcgt gccgccacag aaatcgattc ggcagaagca aaaccgcttc ttactgcgga
   144721 ttccaccttt ttctcgacat agaaaggcac tgccacctct gtggcggcca ccaaaagaag
   144781 catgatgatg gcgatccgcg ggattctccg ggggctgacg cgacgagcca tgctactcac
   144841 tccctgcgtc tcggttagca gaggcagcgc tcaatctcag ttgcgcctcg atgaactcgt
   144901 cgatttctcc atccataacg gcgtccacat ttccagtttc caactctgtc ctgtgatcct
   144961 tgaccatggt atagggctgg aaaacatatg acctgatctg gcttccccag gctatctcgt
   145021 gctgttcgcc acgcatagcg gacaattcct tctccttctc ctccctctga agctccgcca
   145081 gtcgagccct gagcaccctc attgcagtct ccttgttgct gtgctgcgac cgttcgttct
   145141 ggcactgaac aacaatgcct gtgggaaggt gcgtaatcct cacagcactg tctgtcttgt
   145201 tgacgtgctg cccaccagct cctcctgaac gatacgtatc tatcttgagg tctgccgtgt
   145261 cgatctctac gtcctcttca gccatttcag gcactacgtc gactgaggca aaggatgtat
   145321 gcctacgtgc agaagcatcg aacggagata tccgcaccag cctgtggact cctttttcat
   145381 tcttcaggta tccataggcg ttgtgtccat gcatgatcaa tgttgcactc tttatgccgg
   145441 cctcctcgcc tggcagcaga tcggctacct caacggtata gccattgcgt tcagcccacc
   145501 ttgcatacat tcgcataagc atttcggccc agtcctggct ctcagtgccc ccagcgcccg
   145561 gatgtatcga catgatggca tttgcagaat catgaggccc tgaaagcaga tgggaaatct
   145621 ccaccttgtc cactgctgac tcaagagctt cgatctcctc aaagcactcc cctatcgcgt
   145681 cctcgtcatc ttcttcatct gcaagttcag ccagtatctt caggtcttca aatcttgaaa
   145741 cgatcgaatc ataagaggca atttccgcct gcaatctcga tatcttctgc atcagcgact
   145801 gcgctgaatc acgatcttcc cacacccccg gctcagcagc tcgagcctcg cattgagcaa
   145861 ggcgctccaa cttaccaggt atgtcaaaga taggtcctca tttcatcgat tctctcgcta
   145921 agcagctcta tgggttgttc aagctcagct cgcatatact catcaccctt atctgattta
   145981 cttctgggcg ccacagcact tcttgtattt cttgccgctg ccgcatgggc agggatcgtt
   146041 tcttccgatc ttcttgccat ctcttctcgc cggcgctttg gttgatgaat cagtgcctct
   146101 gttggtagat acagcatact tcgggcgacg gaggccagcg gctgcggcag ccgcacccgt
   146161 atcctgaggc gacgccgatt ccgactcgcc ctcaatgcgg attcggactc ggaatacgta
   146221 cctcacaaaa tcttcaccaa tgctcctcag tagatcgttg aacatcatgt atgattcccg
   146281 cttatactca acaaggggat ccagctgacc ataggcccta agccctattc catctcggag
   146341 catgtccata gcctgtaaat gatccaccca tttggaatct atcaagtaga gcatgatagc
   146401 acgttccagc tcacgcatgc gttcggcgcc tactgcagcc tccttttgct cgtatgcccg
   146461 catgaatata gtgagcacgg cttcgtgaag ctctgtccag gaatggaact cccgcgcttc
   146521 aagatcgggc ccggatctca cattgagtat gctggctatt tccatggaaa gccctcgcaa
   146581 gttccattcg ccgtgtccaa gcttctcatc ggcatacgct ctaaccaaac cttccaccat
   146641 cgtgcctacg agctctatgg cttggtccct caggtcggtt ccttcgagga tcgccgccct
   146701 cctggcatat atcgcctcgc gctgcttgtt catgacatcg tcgtattcga gaacttgccg
   146761 cctgatctcg aagttgcgtc cctccacctt ctgttgagcg cgctcgatcg cctttgtcag
   146821 ggtgggatgt tcgatcgcct catcctcggt ccagccgaaa cgctccatca ttccggagat
   146881 gtagtctcct ccgaagaggc gcatcaagtc gtcctcgaga gacacataga acctggaaga
   146941 gccgggatcg ccttgcctcc ctgatctccc gcgcagctga ttatcgattc tacggctctc
   147001 atgccgctca gtgccgatta catgaagtcc gccaagctct gggacgcccg gtcccagtac
   147061 aatatccgtt ccgcgtcccg ccatatttgt tgcaatagta actgcgccag gttgcccggc
   147121 aagttttatg atctcggctt ccttttcgtg attcttcgcg ttgagcacct gatgggggac
   147181 gcctcttttg gaaagcatat ccgaaagcaa ttcggatttc tcgattgaga cggtccctac
   147241 aagaagcggc tgcccagtgg catgcctctc gcaaatctca tcgaccacgg cctggaactt
   147301 cgctcgctca gtcttgtaga ccgcatcagg caggtcctcc ctgatcatgg gcttcgcagt
   147361 aggtatctgc accaccggca gattatatat ctttatgaac tcattctcct cagtgactgc
   147421 agtgcctgtc atgcctgcca gcttttggta catgcggaag tagttctgga aggttattgt
   147481 cgccaatgtc tggctctcgc gctggacatg aacgttctcc ttggcctcta tcgcctggtg
   147541 cagtccatcg ctgtaacgcc tcccaaacat gagccttccc gtgaattcat ctacgatgat
   147601 gacttcgttg tccttgacaa tgtaatctct atcccgccgc atcaaagcct tagctcttaa
   147661 agctgcattc agatggtgcg tcagctctat gttctcctcg tcatacaggt tgctcacgcc
   147721 tagccatctt tccacccgcg caacccctga ttcagtgatc gacaccgttt tggctttctc
   147781 gtccaccgtg tagtcttctt cctctttgag gctttgagca atctttgcaa agacgaagta
   147841 cttctccgtg gactcttctg cctgtccaga gatgatcaaa ggggttcgag cctcatcaat
   147901 gaggatcgag tcgacttcgt cgactatcgc gtagttatgt ccgcgctgga cacgcccatc
   147961 catcgaagta accatattgt cacgaagata atcgaatccg aactcgttgt tcgtcccgta
   148021 ggtgatatct gccatgtagg cgtccttgcg cgcagttccc tgttggtcat ggacaatcac
   148081 tcctacggtg aggcctaagg cattgtatat ggggcccatc cattcagcgt cgcgacgggc
   148141 cagataatcg ttgacagtga cgatgtgcac accccggtct tcaagggcat tgaggtaagt
   148201 tggcatggtg gccactaggg tcttgccttc gccagtcttc atctcggcaa ttttcccctc
   148261 gtgcagcact atgccgccca tcacttgcac atcgtatggg taaagtccta tggttcgccg
   148321 ggcgacctcg cggactgttg caaaagcctc tggaagaatc gagtccagtg gctcccctcg
   148381 ctctattctg cctttgaact ctccggtgcg ggcgcgcagc tgttcatctg tggcccgctg
   148441 ggcctcgggg gcgagagccg ataccgcatc taccctggtg cccagatccc tcagttcctt
   148501 ggcattcaca tcaaatatcc gcttgaaaag gtttaacacc catggccacc tcgcttgtat
   148561 ctttgccgac taagcaatta taccacatca catctcccgc catgttctgc tgcagcaagc
   148621 agtctgactc cgccagagac aagggagtgg cctccaagtg tcaccggcag cccggcctca
   148681 gcagccgcct tcacgaaccg cgcaacgcca ttatccgcca acctatcata gaggaaaaga
   148741 tcggacgcga atctctcctc ctgcgacaag tccgcccgca tgtggaagaa aagaactcgc
   148801 gtatcgatga atgctgcgtc acagcaccat gcaagatcag cgaagaaagc ctccggtcca
   148861 actgcttcta tgtgcttgcc gatgaggctc cgcgcgcccc caggcacgtc ccggccaaga
   148921 gctctcatgt tgcgctcctc agaaaacact ctcagcctgc aacgggtgca agtattcagg
   148981 tccattgtcg tagccgggga gactcgtccg aagaggccaa cctcggcaaa atctctgcct
   149041 aacacctccc tggcctcggc aagacgactc atggcaggct ctatcgggcc tgagcgtatt
   149101 gcatcgagta cagccgtctc ttcgggggca agctgaggga tgaccgacgc taacaccagc
   149161 aaatccgagg gcgtgtccac gtcgaaaacc gatccgactg aaggcggcaa cgccaccaga
   149221 tccattccgg cggcaccggc aatcataggc acagaattgt cattcgacgg aagatttagg
   149281 cgagcaatag cgtctgaact agcggttccg aacatgtcgg ctgagaagag gttgttggcc
   149341 acaatggcct cagggttatt cttgaccatt tccgcaaagt tcacaatttc cgggacagtc
   149401 atgaagaagc ccgaacctcc acccatgtag acaaatccca tggcggcccg ggcctgcaac
   149461 gcctgcacaa gccttgctcc gaaatggtag ttgtcacagc aacggtcgag ctcaacagat
   149521 actccgtcga tagcaccagc tcggtcggca agcgcaggtc cgtcagttat caacacgaca
   149581 tcatcgaata cccccgacga cgccaatttt ctgcagagag atatcgccag ctcctctctg
   149641 acccgctcaa cccatccgct gatttcgccc tgccgcctcc cgccttcgaa aaccagtgcc
   149701 accagtccgt ccacgatgac cacccccgtc acacgccaaa tgacttccaa atatatcggg
   149761 ggcgatgcaa tatgcaccgc cccgctacgc aggatccccg cgcagtttag aattcgggtt
   149821 ctatcaaacc gtagttcccg tctttgcgct tgtagaccac attcacatct tcagtggagt
   149881 catccatgaa aacgaaaaag tcgtgcccca agagttccat ctgcataatg gcctcttcaa
   149941 ccgacatggg tttcatcgca aatctcttaa ccctgactac tttgagctcg gagggttcag
   150001 acgcatccgc agcagcccca tgaccgacag cggattccac cagcctattg ccatcctgcc
   150061 ttagcttcct atttattcta gtcttgtgct ttcttatctg cctttcaatc ttgtccacag
   150121 cgccatcaat cgatgcatac atgtcaccgg ttcgttcttc accacggagc agcagaccat
   150181 cgacttgaat cgttacgtcc gctgtgtgaa tgcctcgttc aactgccatg gtaacctgag
   150241 catccacaat ctcgcgctcg aagtacttct ggagcttatc aaccttcttc tcagcatagg
   150301 cccgtagagc atctgtgaca gcaatattgc gacctacaac tgtgatctcc attcatacca
   150361 gccccttccg aactagttgt ggcgcacatc ggctgcttca gtacttcacc ctttgtggga
   150421 aaaatcctgc ggcagtcgga gaaatgcgtg tccttccaat gcagacgttc agccgtgaca
   150481 gcactcgaag ctctcacgcc tcgtgaccgc gtcgccggct cacttctcgg gaaacgaatt
   150541 tggtaacctc cccaactact agcatgatca aagccaatgc cagagagttg acgagctttt
   150601 ccccgtgcac atccacaaga tcaaagaagc cgcggatggg cgccatgtac acgactgcca
   150661 actgtagtat ggctgatgca acaaaagcca tgactagccg cctgttggta aacatcccca
   150721 ctttgaacaa cgaatcaagc tccgatctga aattgaaggc gtgaactagc tggcagaaac
   150781 tcatagtaag aagacacata gtccgagcct ccgcgagact tgccgcagac gtatcgagcg
   150841 gtccgggcca tccaagcccc atagcaaacg acgacaggcc cagcaacgct actgatatcc
   150901 catacacaac tagcctgctg acggccgcat gccccaaaac atcatcgttt cctctgcgag
   150961 gctttctcct catcaaatca ggagccggag gatcaactcc aagagccagc gctggcagac
   151021 cgtctgtcac caagttgagc cagagtatct gaataggcaa cagtggcctg ggaagcccga
   151081 gcatgatagc tgcaaatatt actataatct ctcccacgtt gcacgatacc agatactgca
   151141 ccgacttttt tatgttatca aagaccaccc gaccctcgcg aaccgcagtc actatcgtgg
   151201 cgaagttgtc gtctgtgatt atcatgtcag ccgcgctctt tgcgacatct gtgcctgtaa
   151261 tacccatagc cacgccaata tcggcacgtt tcaaggctgg agcgtcattg actccgtcac
   151321 cggtcatcgc tacgatatgt cccttgcgtt tccatgcgtc aacaatgcga accttgtgtt
   151381 caggcgcaac cctggcgtaa accctgactc ggtctacggc atcgtctaat tcctcattag
   151441 acatctgcga aagctcggcg ccggtgatag cgattgtgtc aggcgtcatg atccccacgt
   151501 ctcttgccac cgcaactgcc gtcaccttat gatccccggt gatcatgacc ggcgttatcc
   151561 cggcatcaaa gcattcttga acagcggttt ttgcttcagc ccgaggcgga tcctgcatgc
   151621 caattaagcc aagaaaaacg aggccattct cgatagtgtc tacgctgatc tttgggggca
   151681 gctcgtcgaa ttctctttga gccaccgcta acacccgaag ggcagatgag gccatctcgg
   151741 cgttgactct acggatctcc tcacgcaaag cctcagtaag cggaattatc tccgtcccat
   151801 catatactga agagcaaact ttaagtagtt catcaggcgc acccttagta caactgagat
   151861 aggcttcggg aagtcgatga atggtggtca tccgtttcct ttcagattca aatggcagct
   151921 cctctatcct aggcgcccac tgggtcaggt taactttgtc gactccggct ttgaggcccg
   151981 caacgactat cgccccctct gtgggatcgc cgatcatctc cagcctaacg ggcgagtcat
   152041 caagactttc ctcactccca aaagtttcta tctcgacaga tctcacccta gcatcattgc
   152101 acaacaccgc gctcgcaagc atccgcgtga ttgagggatc ctcagaaaca tctatctctg
   152161 tgcctgattc tatgaatgct ccctcagcgg aatatccgtc cccgctgacc tccagaaccc
   152221 tgccgggcac gtagaccctc ctaaccgtca tctgattggt ggtcaatgtc cccgtcttgt
   152281 cagagcagat tacatcggcg cttcccaatg tctctactgc aggaagatgt cttataattg
   152341 cgttcttggc cgccattctt tgaactccta tggaaagcac tattgttact atagcaggaa
   152401 ggccctcagg tatcgcagca actgccaggc tcactgctgt cagaaacatg tcgaaagcgg
   152461 cctctcctcg gaaaacaccg gcggcaaaaa ccccaacgca caacagaaca gctgcgattc
   152521 ccagcttctt cccaaccgct tcaagtttga cttggagagg ggtgggctcg tccttgcctt
   152581 cctgcagaag agcagcgatc ttccccatct cagtagacat tccagtggac actacaactg
   152641 caacgcctct tccgtacgac acagaactgc ccatgtaaac catgttcacc ctgtcgccta
   152701 gcggagcatc ttcgtccaac accgccgatg cacaaagctc aacaggttcc gactcccccg
   152761 taagcgacga ctcatccacc ttcaacatgc cggactcgat gagcctcgcg tctgcaggaa
   152821 cgctgtcacc agcctcgata aggattatgt cgcccggaac cagagtcgac gcgtcaataa
   152881 ccctcggctc accggaccta atgactctgg ccttaggtga tgccatcttt ttcagcgctt
   152941 caagcgcatt ctccgctcgt tgttcctgaa ttaccccgat gacggcgttt actatggcta
   153001 ttgcaatgat aactaaagca tcctcgactt cgcccacaac tccggacaca ccagccgata
   153061 ttaggagaag aatcaccaag aaatctttga actgcgccaa gaactgttgg aaaatcgtcc
   153121 gtcgcggcgg ctctgccaac atgtttgggc catactcttc aagcctggcc ctaacctcct
   153181 catccgaaag cccactttgc cgcgaggttc ttagttctac gagagcttcc ccataaacaa
   153241 ctgcgtgcca cggttttgcc gtatccggtg tcaacaatca tacctccata taaccatgtc
   153301 tatagataac attatgcccg ccaccgcgcc aaccgtctct tgagaaaaac tgatggcaac
   153361 ggagttgacg ggctcgctca tcctgactgg atcaacaaac cctcatgccg aaattgccaa
   153421 taccatgatg atgcctgcgt tcaaaagcaa tctgcgcgcc ccaagcgcgc tcacggctgg
   153481 atttggagtt aattatatat cctgagaagt cctgtgcgca accaatgtct taaccaaaac
   153541 cagtactggg ccaccgctag ccgctgatag accagctatg atctccaggc tcgggccgat
   153601 cgcaaaaggc cgcattgaca ggagccatct gatactggaa agagaatacc gcccacagtt
   153661 cattttccga tctgagttgc gcttcgacat gatgaactgc taaacctaat ctccgtgccc
   153721 ctcaaagaga gactcgaaag taaggcctgt aaccctcatg ctcggctgct tcccggagcc
   153781 tcaaaccaat ccctctcatc cgccactgac acgccaaatg tcagaacgta tactttcgcc
   153841 gccccggcca tcaggagagc cctggaacac ccgtcagcag tggcgccaga agtcagaacg
   153901 tcatctacga gaagcacatt gcgttgggaa acgagagctg gaaaagggca caagaaagcc
   153961 tgtatcacgt ttgctcggcg atcacgccta tccaaaaatg tctggctgcc cgcgcttcgc
   154021 tgacgttgga gggctttggc ctccaacgga atccccaaga gccgtcccac atcttctgca
   154081 agcagctgag cctggttgaa accgcgccta gccgccttct cagggtgaag cggaaccggg
   154141 actacacact ggcattcccc ggccatcccg tcgccgatgg ccacctccgc catcattcgg
   154201 gcaagcggcg caacgagttc cgttcgcgcc ctgtatttca gctcatgaat gtgatcccgc
   154261 gccgcgccat tatatactgc aacagccctt gcgcgctcga agtagtgccg agtcgtcgca
   154321 catcccgaac atacctgatt ctccgggttt gaagcaggtt cgcctcgtgt ttttggcccc
   154381 agcagcctga ggggagtacc acatagcgaa cagacggggg gaaggacata agcgatcctt
   154441 tccttgcact ggctgcacat tccacttcta tcagctatcc gcccacaaga cgcgcaacga
   154501 agctcccctc cgaacagcac tgtgtccaca aaatcccgaa agcgtgacag ctgaatcatg
   154561 cttcatacct cactcccagc atttaaaaag ccccgggacg ccgcatcccg gttcatttcg
   154621 tcaatcatct cccgcgcagc ctgaatctgc ctgttctccc ttgagcacgt gaaccacaca
   154681 ctccctgtcg gattcgatgc cgtcctccct acccgcccag ccatctgcac aagcgccctg
   154741 cagtcgaaga cagtctccca atccgcgtca gccactatta catcgacccc gtctactgta
   154801 atgccccttt ccatcaccga tgtgctgacc agaacttcac attcaccttc tgccaacatt
   154861 ctccttttgc tgtcgcgcat tgcatcgcct gaatggaccc actttgtcct cgcatctggc
   154921 acagtttgag atagatattc cgccacttct ggggcacgat ggcgtgtggg aacgaaaacc
   154981 aagaccgggc atcctcgttc gagactagct gtagcaagtt cggcaacgac aggttcctgg
   155041 ggcttgtccc ccatctcgat caccacaggc attggcagag gatgaccatg atgacgcgcc
   155101 gatatcttga aacagggcag agtacccgcc tttattctgg ctatgtgccc taaagacggc
   155161 gtggccgtga gcaccaccgt acgtccgcca aaggccaatg acctgtcgac cgaagcggca
   155221 agcgcttcag agccgtaata tgggaacgca tccgcttcat ctattatcac taggtcgaaa
   155281 actccgtaaa atcgcatcgc ctggtgggtg gtgagaagag ttatgtcggc tccgaaatac
   155341 cgttctgaag agcctccata gaagacgccg caccgagccg ctggaaaggc cgactgtatg
   155401 cgcggagaca gctctgccac tacgtctctg cgcggcgccg cgtatgccac acgcttgcct
   155461 gcctgcaggg cagcagcgat taggccgtac gagacttcgg tttttcctgc gccacatact
   155521 gcccacacaa tagcctcagg ggattcgtcc gtctcgtacc attccacaag ggcccgagca
   155581 gcatctacct gagcaggagt aagcgcgtgc ggcattacca tattcgcctt gtcggcaact
   155641 gacccgatga ttgaggtgcc aacgtaaggc gggttgacaa gatagatagg cgtacactct
   155701 ctaacaattc ccatattcct gcactccgaa cagactgcac aaatgtctct gccgcatctg
   155761 gcacatggaa gttgaagaaa aggccctctg gaaccgcatc tgttgcattt gaagccgccg
   155821 atgctggtta tgcccactcc tgggaggatt gtggcattgc cgtccaaaac catggcaccc
   155881 agcagatggt ccgcacggtc cagaccgcac agacctgcat cagccagagc ccgtttggcc
   155941 tcctcgtcta aaagcgcacg gcctcccagt tcgtctatgc atgccttcaa gacatcagac
   156001 ggcggcaagt ctaatccatg aacagacgaa tcaaatgaag acgccgacag gagcatcggc
   156061 cgcggaagac gagaccgacc gccgtatccg acagctgata gaacagactt ggctatgcgg
   156121 cgcccacgtt tcaatgcccg ctcgggcggg gcatcaatgg gaatcgatgc attcagctcg
   156181 cgcgccacaa gctctgcaat ggctacaggc aggggaggcg tgagaactac taggtcggtc
   156241 ggggatcgcg tgttcaagaa catccattcg aactcgggcg aacgcccgaa tccagcccaa
   156301 accttgggct cggagaatat caggtaggaa tggaacatgg cgtcggccca cctctggagg
   156361 tcatgatgcc atttagcttc tgcataggcc accattttcc tccattaagc ggcaaaatca
   156421 tcctggctaa caccatctgt tgctagcatg tctccttctc atactgtaga ctctgccaga
   156481 tctctaagtc tctcagccag cattgtcctt ctttttagca gggatgcatt ctttatgccg
   156541 attgcaagcg ccctcttggt tcctatcata accacagctt ccttcgctct ggtgaccgca
   156601 gtatagatga gattccgttg cagcataacg tagtgctgag tggtgacagg aatgaccacc
   156661 gccgtatatt cgctcccctg actcttgtgc actgacacgc agtaggaaag gactagttcg
   156721 tccaggtcat cttggagata cgtgacatta cggtcgcctt cagcttcagg atatctgact
   156781 acaacctcgc cttcctccgg atcgatccac acgatccggc ccatatcacc gttatacacc
   156841 cttcgctcat agttgttgcg aatctgcatt accttgtcac cacgcctgaa aaccgtagcc
   156901 ccgtatctta gctcggatac cccttgcctc gcagggttca gagcatctcg caggagtaca
   156961 tttagattgt cgacgccggt gacgcttcgc cgcataggcg agaggacctg aatgtcatca
   157021 acagggttac acttgagata cctgggaagc ctcgatttaa ccgtatcaac gacggcctga
   157081 gccacctctt caggatcttc cttgtccaca aagaagaagt cccgtgcgcc tctgaggtgg
   157141 ggaaattcgc cccggtttat tcggtgcgca tttgaaacga tcatgcttcc tggagcttgc
   157201 ctgaaaacct catccaagac gacgacctcc accacgccgg aggcaatgat gtctcgcaac
   157261 acgtttccag ggcccacaga tggaagctga tcgacatcgc caacgagaat cagcctagtt
   157321 ccaggtttaa cagccttcag catgttgttc atgagaagaa tatccaccat agacatttca
   157381 tccacaatca gcacatcaca ctcgagcggg ctcgataccc cccgtgcaaa acgccatccc
   157441 tcctcgccgg gttcaaactc caaaagccta tgtatagtct tcgcctgtct cccgctcgct
   157501 tccgaaagcc tcttcgcagc ccttccagta ggcgccgcca gcatcacacg gagtccccta
   157561 ccttcgaaaa ggtcaatcat cagctttagg gtagtagtct ttccggttcc agggcccccg
   157621 gtaagaacca tgactccaca ttgaaaagcc cggactattg cctgcttttg ctgtgctcca
   157681 agggttaatc ccgcctcagc ctgagcccga ccgagttctt catcggatag cgcatctaac
   157741 tgccacatcc tgccatgatt cagttcccaa aggcgtttcg caacaccacg ctcagaatga
   157801 tagaatgggc ccagccatac cgacgattcg cgctcgcaga atacctcgcc cgattcctcc
   157861 agggccccaa ggccctgctt aaccaagtcc tccgaaacgt ccagctcggc tgcgacctca
   157921 cgtataacat cctccgccac cgagtagcaa tggccatcat ctgacgcctt ggccagccag
   157981 tacttgattc ccgagttgac gcggtatggt gaatccttgt cgaccccgac ctccctcgct
   158041 atcttatcgg cggtcttaaa acctatcccg taaatgtcat cggcaagttg ataagggttt
   158101 ctactaacaa tcgctacagc atcgtctccg taacgcttgt aaatcttcgc agcatacgtg
   158161 gggctcaccc cgtgcgactg taggaagacc atcactttct caatagactt atgctcttcc
   158221 acggctttca caatctgcgc tgcacgtttc gggccaatac ccggcacttt caacagggcg
   158281 tcaggacagg actggatgac ttctaacgtc tccatggcaa aatgctctac gattctcttc
   158341 gcggttacag gtcctatgcc ggggatcaac ccggagccaa gatatttctc tatcccctca
   158401 acagtagccg gcgcaattac ttccatcttc gcgactcgga actggcgccc gtatgacctg
   158461 tgaacaaccc agtctccagt caacttcaca gcttggccag gcgtggctga gctgaatgga
   158521 ccgacggccg tcatcattcc gctatccgtg ctaatgcggg ccacagtata aagagtatcc
   158581 tcgtcgcgga aggtcaccct gtcgatgatt ccctctacaa gatccagctt cgttcacctc
   158641 accacggtgc caataccacg actggggagc gctcatgccc cccagtcgtg accgaatatt
   158701 gcaggcgcat gccttacatg cgcttacgca cctcttcaac catgtccaac gcctcccaag
   158761 gcagatcgag gtcgggcctt ccgaagtggc catatacgga aacctgcctg tatatagggc
   158821 gacgcagatc gaatttgcgt attatcgcct caggtcgaag gtcaaagacc tcacgcacta
   158881 tcctggttat ccgttcatct gggatagcgc ccgttccata agtgtcgacc gaaatcgata
   158941 cagggcgggc gactcctatc gcataggcaa tctgaacctc acatttgtcg gccacacccg
   159001 cggcaacgag gttctttgcc acatgcctcg cggcataagc tgcagacctg tccaccttgg
   159061 tggggtcctt gcctgacaga gcgcctcccc catgccttgc catgccacca tacgtatcaa
   159121 caatcaattt ccttccagtg aggcccgagt cagcagatgg tcctcccttt tcgaaccggc
   159181 ctgtaggatt tactaagatc cgggtatcgg catcgatcag gtcacttggg attacgggct
   159241 ttataacata ttcaataatt ccggaagtca gcgtttcctg ggaaacctca tcatcatgct
   159301 gagccgatac tatgatcgta tcgatccgaa ccggcctccc gttttcgtac tccacagtaa
   159361 cctgtgtttt tccatccggt cttaggtaat caactacacc ggtctttctg atctcagcaa
   159421 gccgcctggc aagattgtgc gacaacgtta tgggaagagg catgagctcg tcagtttcgc
   159481 gcgaggcata accgaagacc ataccctggt ccccagcgcc aattaggtcg cattcatccg
   159541 cacctcgtgt gccgccccca cacgagcttc taacctcgag cgccctgtca acaccaagac
   159601 tgatatccgg cgattgctgg tctatggacg taatgaccgc acacgtatca ccgtcaaatc
   159661 cgaattttgc tctggtgtac ccgatatcgc gtatggtttc ccgtgtaatc tcgggtatct
   159721 ccacgtagca gtggcaactt atctcgccca taaccaatac aagacctgtg gtagcgcata
   159781 cctcgcaggc cactctggcc cagggatcct tctcgaggat agcgtcgaga acagcatcag
   159841 cgatctggtc gcaaacctta tccggatggc cttcagtaac tgattctgat gtaaaaagac
   159901 gtttctcgct caccagcaaa ccctccaaca atgatgcatg gccaggtagc gcaaacgctg
   159961 gcatgacgct gaccggccct acagcatctt accatacttt agcctagcaa ggaaactgac
   160021 gcttctccat ccttttcgca agtcattcta aaggctggag ttcccggtct gcatgcct
//
LOCUS       DAOSYL010000002       136572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_72_length_136572_cov_7.585656, whole genome shotgun sequence.
ACCESSION   DAOSYL010000002 DAOSYL010000000
VERSION     DAOSYL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 136572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 136572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136572
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_136572_cov_7.585656"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1415)
                     /gene="coaBC"
                     /locus_tag="PLU15_00625"
     CDS             complement(219..1415)
                     /gene="coaBC"
                     /locus_tag="PLU15_00625"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOO29457.1"
                     /translation="MTGTCVLGVTGGIAAAKASLLASMLVKQGLDVHVIMTEAACEFV
                     APLTFRTLTANPVSVDMFAEPGEWNVKHVSLAEAADVFVVAPATANFIGKVASGIADD
                     LLTTTIMATRAPRVIAPAMNSGMYANPIVQRNIETLSALGYRFIGPESGRLACGDVGA
                     GRLAEPERILDFVLHVCAGTGRPSLAGLRVVVTAGPTREPIDPVRFITNRSSGKMGYA
                     IAGAAAVRGAKVTLVSGPVFLPAPEGCEMVRVETACQMRDATIDAAQEADVVVAAAAV
                     ADYMPASFSESKIKKSSGFGSLELSRTPDILAELGSAKGERVLVGFAAETDDLLSNAE
                     RKLSSKNLDMIVANMVGGEDSPFGADHNTVTILCPGAPPETFPAMSKLVLAHEILDRV
                     SAICHH"
     gene            complement(1547..1732)
                     /gene="rpoZ"
                     /locus_tag="PLU15_00630"
                     /pseudo
     CDS             complement(1547..1732)
                     /gene="rpoZ"
                     /locus_tag="PLU15_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            complement(1846..2496)
                     /gene="gmk"
                     /locus_tag="PLU15_00635"
     CDS             complement(1846..2496)
                     /gene="gmk"
                     /locus_tag="PLU15_00635"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOO29458.1"
                     /translation="MLKRLTEVPRIRGNLIVISGPSGSGKNSALAAVRSKVPDLVYSV
                     SATTRRPREGEIDGVHYFFLTREEFLAMKDAGAFLETAEFCGNLYGTPRSFVEGKLDA
                     GEDVIMDIEIRGADQVRAKMPDAVTIFLMPPSLAELEARLAKRGADSPEATRRRLRKA
                     IEEIPAVLKYDYVVLNYDLEQAAEQIRSIIVAERARVGRCDCEHFIRGFEMESQIF"
     gene            complement(2507..2773)
                     /locus_tag="PLU15_00640"
     CDS             complement(2507..2773)
                     /locus_tag="PLU15_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOO29459.1"
                     /translation="MDIKLVNIGFGNMVAANRLVAIVSPESAPIKRIIQEGRKRGMLI
                     DATYGRRTRAVIITDSGHVVLSAVQPETVAHRVEMKEADVDGRD"
     gene            complement(2784..3095)
                     /gene="mihF"
                     /locus_tag="PLU15_00645"
     CDS             complement(2784..3095)
                     /gene="mihF"
                     /locus_tag="PLU15_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HOO29460.1"
                     /translation="MALPELTDEQKREALKKAQEVRSKRAQIRAELKKGNMSLEEVLA
                     NADDEVIGKMRVAYLLESLPRIGKVRARQIMEEIGIDENRRVQGLGVRQKEALLNKLA
                     K"
     gene            complement(3143..4021)
                     /locus_tag="PLU15_00650"
     CDS             complement(3143..4021)
                     /locus_tag="PLU15_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOO29461.1"
                     /translation="MLMSMTGYGRAANSGPEGDVTVEIRSLNHRYFDVSLRMPREFSP
                     VEERVRSLVAERIARGRIEVSVSVDWHDGSRRTVALDTCLARAYAEGLARLAHELALA
                     PISSVDVLSGLPDVITLSEAKIDVDILWNMVQPALSDAIDQVVQMRKEEGARLERDIA
                     GRIEMIEMRVDEVGRRAPEVVDSYRAKLARRVSEMIGDVRVDEARLATEVALFADRCD
                     ITEETVRFKSHIRQFRDTISESGDCGKKLDFIIQEMNREANTIAAKSQDSFISALAVE
                     IKAELEKVREQVQNVE"
     gene            complement(4193..7015)
                     /locus_tag="PLU15_00655"
     CDS             complement(4193..7015)
                     /locus_tag="PLU15_00655"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOO29462.1"
                     /translation="MSELRAWHSVSAGVVERELETSASSGLTSKEARARRGAYGPNQI
                     RAGRRQSPLVILLNQFQDFMVLVLLGATAVSAFLGEYRDVVAILAIVVLNAVLGFVQE
                     FRAEKALEALRKLSAPKATVLRDGRATQIDAVDVVPGDVIILNPGDRIPADARLAESY
                     GVEVDESVLTGESRPVKKDASCIVRQSAPVVEHRNMLHAGTVVTRGRAIGLVVATGME
                     TEMGRIAGMIEDVSDGDTPLQRRLSHLGRWLVAGCLFLCALVAGLGVARGEPIELMFL
                     AGVSLAVAAIPEGLPAIVTVSLALGVQRMSARKAIVRKLSAVETLGCATVICADKTGT
                     LTQNQMTVVEIDLIGRNIQVTGSGYSPEGEFLEGGETIDPLNDGIFEDLLLCAALCND
                     ASLVRQSAEERTTGSMGRGRGRGYGYGPGRSWDVVGDPTEGALLAMAYKGGREIMQKV
                     RSFTRVAEIPFEPERRMMAVACSGPSGVFTFVKGAPQVVLELCEGRYVGGHVEGGIVL
                     RDRRFDGAVLARNSSMASKSMRVLAMASAPGNLIEQIRVGQSHALSGLTFLGMVGMVD
                     PPRPEAARSVALAKKAGIATLMITGDHAATAESIAKQVGIMESGRRILTGEELDAMSD
                     RQLEEVISEVAVFARVAPAHKLRVVRALRAQGHVVAVTGDGVNDAPAIREADIGIAMG
                     KNGTDVAREASSMVLSDDNYATIIAAIEEGRSIYDNIRKFIRYLLACNVGEVLTMLLA
                     VAGGLPLPLTPIQILWMNLMTDGLPAMALAADPTDPNTMQRAPRCPSESLFAHGLGLK
                     VLADGVFISICTIACYISAGAFLGHDLDTARTMAFSTLVMSQLVYVFRCRVEERSALE
                     SGRRPSNRFLLGAVGISVVLQVLGVHHHVGRAFLGTCELSVVDWVLVLFLSGWSTFLA
                     SAVRAVQRAIRRRCTVFRVNKAGQGT"
     gene            7142..9025
                     /locus_tag="PLU15_00660"
     CDS             7142..9025
                     /locus_tag="PLU15_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOO29463.1"
                     /translation="MQIDGITLYAVTREIERELTGTRIDKIQSPSHDCITLDLGRGRA
                     PSLLISASPTSSRICLTDSVPPSLPTPTAFCMLLRKHLVGGRVVSVRQDGLDRIIIMQ
                     IEGWADTDPSDNKLLIAELTGRLSNIILVRMDGRILDAAHRVDTSRNRYREITPGASY
                     VPPPPIEKADPFRVTAEEFRAIIQRQSAQIRARAEADAASKRRPSRRKDSVANLISSG
                     FAGIGPNHSREIAWRAGIDPDRSALELHADDIGLLWAQFEDFRRAVKVGDFLFEASFD
                     RHSGKPTACCVWGLSGLSGEYRVERFASASEMIDRCYNRAEERERLELTRSNITHKVE
                     ARLARSVRKLEAQRRDLAEAEDSSAFRRKGELLTANIHSLGRGPLREDRVRVVDYYDP
                     AAPTVEIEVDPSLSASENAQSFFARYSKAERARKAVTANIAATEEEVAYLESMLAMAT
                     IAETEETLNGITEELAALGFIQPRQGRGSARRAKKKHKERLPMPTSFRAASGDEIYVG
                     KNNLENDHLTTKLARPHDLWFHAKDIHGAHVVVRRRPNQEVPDETLRAAALLAAYYSQ
                     ARMSSNVAVDYTEARNVRKPRGARPGMVIYEAHKTIWVTPSREELSNALGFNGDTCPG
                     NED"
     gene            complement(8960..10252)
                     /locus_tag="PLU15_00665"
     CDS             complement(8960..10252)
                     /locus_tag="PLU15_00665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOO29464.1"
                     /translation="MGGAAQDSGAVSMMGNGGSKKADGTGRVDLAVRRAAALEVLLAV
                     LASCVICTGCAPDCTWLRFSEGRAAYPCSDGSRLEDVWQRTACESLQSRFTLAAAGDV
                     IFDRGVRAVCERRGADWPLSAVAPIFSSADAGFVNLETSLAVGGRPIPGKGIWFRSDP
                     KFAQELAKAGVNVVTVANNHVLDYDEAAFAETLAALADSSIAVCGGGFDLDEARSPAI
                     VSAGGVRIAFLGYSELADIFWTYSYPRSFRAGLDSPGVAPMDEKMIVQDIRKARAVAD
                     HVVVSYHWGEEYARMPNTRQMQIAHMSIDAGASLVLGHHPHVLQPVEVYNGGIIFYSL
                     GNFVFDQTKPDTVESMIARVTFSPSKIVSAQILPMRIEECRPRPLVGWAARTVLDRLI
                     RDSAWLGAVVAPTGGRGLILIPGTSVPVETEGVGEFLP"
     gene            complement(10230..11189)
                     /locus_tag="PLU15_00670"
     CDS             complement(10230..11189)
                     /locus_tag="PLU15_00670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO29465.1"
                     /translation="MQVLFFSPGEADDRTRLDLYLAKESVAPSRSYIQKLISDGRVTV
                     NGLVETRSSYRVRAGDAVEVRVPPPEPLCPGVPAPESIALDVIYEDDDLMVINKPRGM
                     VVHPGVGRSTGTLVNALLGRGTQLSTVGGELRPGIVHRLDLDTSGAIIVAKNDATHLA
                     LASQLASGDIDRNYIAIVRGNIREDCGVIDAPLGRHPVDRKKRAVRPDGRPAVTRFTV
                     LERFGDYTLVGIELETGRTHQIRVHMKYIGRPVAGDPVYGGVAGELGLEAQALHARSV
                     EFTHPVAGERVRIEAKKMPDDMRAALALLRSREPSGDGRCGAR"
     gene            complement(11193..11987)
                     /gene="lgt"
                     /locus_tag="PLU15_00675"
     CDS             complement(11193..11987)
                     /gene="lgt"
                     /locus_tag="PLU15_00675"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO29466.1"
                     /translation="MHPILFNVGKFPVYSWGTCLAVAFVVSALYAIWQGKKKEIDPDD
                     IIDLALYACIASILGARIFYVLLNITSYLDNLVSILWMRDGGLSFFGGLAGGVLAGVL
                     LAKKREIPLGKLADVVAPAIAIGYAIGRLGCLLNGCCYGKVAVDLPWALACGTDGAPR
                     HPTQIYAIIYSVIIFFILRAWEGRKRFEGEVMWAYIGLYSVARFIVEFWRVGVRDYFA
                     PLTATQMLCIVLAVLGFGMVYVKSKYPESVAEASTDGAVSKSSVRG"
     gene            complement(11987..12436)
                     /gene="lspA"
                     /locus_tag="PLU15_00680"
     CDS             complement(11987..12436)
                     /gene="lspA"
                     /locus_tag="PLU15_00680"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOO29467.1"
                     /translation="MSLFLTSAWVILLDQITKTIVTRQIAPWDNIECLGGLLQITNVH
                     NPGAAFGILQNKQILSIVAAIVAAVLILLFYPRIPRHRRSMRFGMALALGGALGNLID
                     RVKYGSVIDFIDVGFWPVFNLADSAIVVGVALILLGMARDPSVRRSN"
     gene            complement(12528..13094)
                     /locus_tag="PLU15_00685"
     CDS             complement(12528..13094)
                     /locus_tag="PLU15_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOO29468.1"
                     /translation="MEPEKIDSCKSRLIELKAQAESRVRDVEETAGFDTPESEWSGEL
                     SSYDNHTADSASALEMRSINLGLIHHGRDIVAQIESALERIADGSYGKCDQCGRDIPP
                     ERLDAIPWTTSCAQCKADSLSPSGRSGPRPIEEEVVRPPFGGRDPARAYDDPGIHGED
                     IWDALAEYGTANSPQDEEAGNSGDVDSF"
     gene            complement(13111..13410)
                     /locus_tag="PLU15_00690"
     CDS             complement(13111..13410)
                     /locus_tag="PLU15_00690"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HOO29469.1"
                     /translation="MRRKRRPGEVRALMSQIMQLSRRLEDSSVAEYVEFMRNPRRVIW
                     SNFLAGMARGFGIAIGASVLTALFLYALSVVISLNIPIIGNLIAKIVKIVKDSGV"
     gene            complement(13455..13955)
                     /locus_tag="PLU15_00695"
     CDS             complement(13455..13955)
                     /locus_tag="PLU15_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOO29470.1"
                     /translation="MLTPLEVHSKQFQTKFGRYNAAEVDDFLDLVGQCYDQLYRENAE
                     LKEKLRLLSEQAQYEPEERDEDIAETIKQTLLMAQKAAEDARRNAEEKASLIVENAER
                     EAAAVLDRTEERVRAQQKRLEDLYAQEAAARARFKAMLEAYLEMLEDTHRDAEGFVGG
                     VTRKYE"
     gene            complement(13969..14730)
                     /locus_tag="PLU15_00700"
     CDS             complement(13969..14730)
                     /locus_tag="PLU15_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOO29471.1"
                     /translation="MYDDSAVLRAAREAAQSGVPVRLGFIDPNEQDDAAEVLASIGDV
                     TPRFYGGYRGAARKLLVIMPSGYLDEVFNAGISYFGLRGVGLSLDGGSGSDAAIAELR
                     ALGIPRDHIGDVFYGGNEWQGVVLASVFGDGSNERELEGSGARLVELDPAEVVFPGVV
                     PKKIRATVASMRLDAVAGAGFPASRSRLAQEIKAGRFKVNGEIVQSPSVKVSEGDVVV
                     SRGRGRLVVEEVFGETKRGRVSLMLTRYSANAPFS"
     gene            complement(14776..15477)
                     /locus_tag="PLU15_00705"
     CDS             complement(14776..15477)
                     /locus_tag="PLU15_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOO29472.1"
                     /translation="MSSIADNLGILQDRIAAAVGRAGRDPSEITVVGVTKNVEAQRIA
                     EAVAVGIRDFGENRVQEAAKKIPLVEADVSWHLVGHLQRNKAKQAVELFSLIHSLDSA
                     RLAEELNRRCEMADCVIDLLLQVNVSGEDTKFGIPPEMARDALLEMAKYERLRVCGLM
                     TIAPYSDDPEDARPCFARARKLWEDLGKLSISNVRMDELSMGMSGDYEVAIEEGATII
                     RVGTAIFGPRRRELS"
     gene            complement(15566..16858)
                     /locus_tag="PLU15_00710"
     CDS             complement(15566..16858)
                     /locus_tag="PLU15_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOO29473.1"
                     /translation="MRAPEPRRARVEERTPAKRTQKSFAIAVIVVVVAVLSYSLYAMV
                     SPGLVIRFSESAVARHGSIELSSRGKAIIVRDERVVITPISGIIHFLVPDGEKVAAGT
                     AVAEVRDTAISSSVEANALRIEEELDKFVQEAALREGREKDRLLAIRVQIALKEEELK
                     AYVDRKDAKNANKVHSELEQLHKREAAAEAEVGRIVFDTQAAAAAIAEAKRQSDAAAG
                     RALAVLRSCDASLISAHLDGLEGIFNPRNPDLLLINPADYDAFPHVRAEGDLVAAGSA
                     VFREVQNFRTDLLVFAEFPGECVPAVGARVWVRFPRLALEAVPATVMGAMNRPDMGEG
                     TWAFHVALDRYATTLTSLRSEDIDFITGYVSGTVVPIEALTSKYGVTGVYLFRTGRFR
                     FKEVEVIGSNGTEAVVAGLSEGDRVKLGDGGRSARMPK"
     gene            complement(16930..17436)
                     /locus_tag="PLU15_00715"
     CDS             complement(16930..17436)
                     /locus_tag="PLU15_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HOO29474.1"
                     /translation="MQGCVSEDTTVFLKDSAGRILEVALVFFNAMLDSDAGGDDLGEY
                     KILGRSGWTDLLCVARRNLWEGEQLCHIRTRLGRILLTGEHELYVRRGPVDVVVAAND
                     VKAGDALLMLERPRLGKNAPPLGEEISFRTLGVLSATASTGFSGYVYQLQTGDGTFVA
                     DEFLVHGL"
     gene            complement(17956..18201)
                     /locus_tag="PLU15_00720"
     CDS             complement(17956..18201)
                     /locus_tag="PLU15_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOO29475.1"
                     /translation="MQRASELRVKEVVSVNDGKRLGFMFDVEIDMDTGKVISFVIPSG
                     GKVLGFLGRGGELVIEWDRIRKIGLDVILVDDAGLTL"
     gene            complement(18289..19029)
                     /locus_tag="PLU15_00725"
     CDS             complement(18289..19029)
                     /locus_tag="PLU15_00725"
                     /inference="COORDINATES: protein motif:HMM:NF021093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOO29476.1"
                     /translation="MPTTKKEIVARTITCGLVLFCFTLCAVVLAAQSDAAWGEARLAY
                     NTSNLIRLHVVAPADDSASSCLKLAVRDAILGETSSLLESAKDAREAEARIRANLGRI
                     QRMANEVVARAGFSHSVRVTLGMEQFPAISYGQISLPSGSYLALTAAIGPAAGSNWWC
                     ILFPPLCLVDVTEGCIIEARQDGNRPIGLEFATRSLLFAPTAAEKEAALYHASRATQR
                     ATLAQLNWELPEWICRLLGLSRFDQAKR"
     gene            complement(19056..19829)
                     /gene="sigG"
                     /locus_tag="PLU15_00730"
     CDS             complement(19056..19829)
                     /gene="sigG"
                     /locus_tag="PLU15_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOO29477.1"
                     /translation="MALNKVEICGVNTSKLPVLSSSKMRELLEAVRDGDKEARAKLIH
                     GNLRLVLSVIQRFTNRGEYVDDLFQVGCVGLIKAIDNFDLSQNVKFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLRDIAYRALQVRDKLMSETAREPTINEIAAALGIPSEEVVYAL
                     DAIQEPISLFEPIYHDGGDPIYVMDQVSDEKNTDANWLEGISIKEAMLRLSPREQTIL
                     KLRFFEGKTQMEVADEIGISQAQVSRLEKNALRQMRKSM"
     gene            complement(19994..20728)
                     /gene="sigE"
                     /locus_tag="PLU15_00735"
     CDS             complement(19994..20728)
                     /gene="sigE"
                     /locus_tag="PLU15_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOO29478.1"
                     /translation="MRKRLESIMWAIRLFIIKAIGWFVIVEDSQVRYIGSHEMLPPPL
                     SLDEEAFLMEKLKTGDICVRSELVERNLRLVVYIARKFESTGVGVEDLVSIGTIGLIK
                     AVNTFDPEKKIKLATYASRCIENEILMYLRKTNKIRSEVSFDEPLNVDWDGNELLLSD
                     VYGTEDDLVYQALEEEVDRRLLGEALSSLSGREKRIMELRFGLSDGSEKTQKQVADLL
                     GISQSYISRLEKRILTKLRRQMIRVE"
     gene            complement(20750..21637)
                     /locus_tag="PLU15_00740"
     CDS             complement(20750..21637)
                     /locus_tag="PLU15_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOO29479.1"
                     /translation="MIRIYLDSLLLLAGGTFVLDFLTLWAVGQISRRSTRASKLMRAS
                     VLSASSFVAIVILSDLGIIILSSPMSLTVALLAAGASLVIAFPGMDARELIVALFYRY
                     LLTVMAGGAATAAYSAAGGSRPLSFIASVATVMLVAEAGWGAVHRGMREGLFYVPISI
                     RFDRYSVSLTALIDTGNLLRDPISGVPAIIVEYSAIEPILPHTIKAMLEISDGDFAAA
                     AAAIAESPWSTRFRAVPYSSVGQTRGLMVGFRPSEVRVIDGKKEISTDRAIICVHNAS
                     LCSNGKYRALLNPEILTAG"
     gene            complement(22067..23125)
                     /gene="ftsZ"
                     /locus_tag="PLU15_00745"
     CDS             complement(22067..23125)
                     /gene="ftsZ"
                     /locus_tag="PLU15_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO29480.1"
                     /translation="MFELELEVDQLANIKVVGVGGGGSNAINRMISAGLKGVEFVALN
                     TDAQALRMSNAPRKIQIGEKLTKGLGAGANPEIGKKAAEESRDLLAETLDGADMVFIT
                     CGMGGGTGTGAAPVIAEIAKEQGALTVGVVTKPFSFEGRRRMAQADHGTEELKDKVDT
                     LIVIPNDRLLQVAESQTSMLDAFRIADDVLRQGVQGISDLITVPGLINLDFADVRTIM
                     TDAGSALMGIGIASGENRAAEAARSATASPLLEASINGAKGVLMNITGSSNMGLFEVN
                     EAAEIVSEAADPDALVIFGAVIDDSMGDELKVTVIATGFDVQQAEQPLKARTDRVQIK
                     PLAGSDDLDIPPFLRRAR"
     gene            complement(23150..24367)
                     /gene="ftsA"
                     /locus_tag="PLU15_00750"
     CDS             complement(23150..24367)
                     /gene="ftsA"
                     /locus_tag="PLU15_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOO29481.1"
                     /translation="MGKNDIKVGLDIGSNQIHVVVCERDEDYNLNIIGVGSGPSAGLR
                     RGVIVDMDSAAAAIADAVAHAEEMSGCAAVSVLASASGTHVSSIASRGVVAIGRSDRE
                     VTQEDVDSVLEAARVVAIPPDREVVHVLPREFVIDGCSGIRKPVGMTGIRLEVDASII
                     TGSATAIQNIHRSARRIGLEVEHVILQPIAAAEAVLTLAEKEQGALVIDMGGATTDIA
                     VCIEGGVHHVASIPVGGNHVTNDIALVMRLPVGYSEGLKLNYGSALPDMVGENESCGA
                     DTNISRKKLCQIIEARITETMELVGDELDRARYRDLLPAGVVVTGGASVMQGVVEVAQ
                     EVLGLPARLGVPVGVYGAEEALDNPQYATAVGLVKSVWSESAVIPSADGRRPKSGGFA
                     SEVRQWFRDIFSS"
     gene            complement(24540..25310)
                     /locus_tag="PLU15_00755"
     CDS             complement(24540..25310)
                     /locus_tag="PLU15_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOO29482.1"
                     /translation="MPDSSDMSDMPGLEEEPHAESRPAISFPAVVLLFLVLATVAFLN
                     SPYFLVRAVRVSGSNYLSDYEVLLIAGIPENTNMFLVPTGEIERKLLATPRIRTAKVY
                     RILPDTISIAIEERSTAGYLPYGGYFIDVDEEGYAIGISEAITDPDVPLIVGVAPTWV
                     EVGQPVNPKYEVSVGARVGAALLKQGIPNISEVDVSSCEDIIIRTSDGIKVFIGGARD
                     MESQVRVLDSILASVREQEMSADYIDLRVEAKPVIRSR"
     gene            complement(25392..26675)
                     /gene="murA"
                     /locus_tag="PLU15_00760"
     CDS             complement(25392..26675)
                     /gene="murA"
                     /locus_tag="PLU15_00760"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO29483.1"
                     /translation="METLRIVGGRELRGSIATPGAKNSCLKLLAMSIMASGKCVISNV
                     PRVTDVETMCQLLEALGARVSFTGRTEVTVDPTTIASAAPPDPLVRKMRASVQVLGPL
                     LARFGEVKMPYPGGCAIGSRPIDLHLRGLSAMGAQVSEEHGYIMVRASRLVGTDIHLD
                     FPSVGATENLMAAACLASGTTIIRNAAREPEIVDEQNFLTRLGADIRGAGTDTIKIVG
                     VSELGSTNYSVMPDRIQAGTYMIAAAATRGDVTITGVVPEHLDALAAKLAETGVAVIY
                     TEDCVRVAMGDRPGPIAVKSMPYPGFPTDLQAPIAAYLTVSRGTSTITENIFSNRFRY
                     VDELARMGAHIGVDGRTAIIRGVNELTGARVVAPDLRAGAALIIAGLMARGETTLDGM
                     EHVYRGYDDIQGKLAACGAEVEIVTDSASAAVAEQ"
     gene            complement(26717..27634)
                     /gene="murB"
                     /locus_tag="PLU15_00765"
     CDS             complement(26717..27634)
                     /gene="murB"
                     /locus_tag="PLU15_00765"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOO29484.1"
                     /translation="MTSMVTDEQARQLAKSFNGRIALNEPMKKYTSFQIGGPADMLVQ
                     PRDLQSLSVVLRWAWNEGLPLFVLGNGTNLLVADEGIRGVVVRIGPGLDSVSFDGERV
                     RAMAGVSMPALARACAERGLAGLEWAVGIPGSLGGAMAMNAGAYSHTVGDFTREVTTM
                     LLDGSISVKSCEEMQFAQRSSRLSHGDLIACEAVLVLEPGDPESIRCQMSEYMEDRRI
                     KQPLHLPSAGCVFQNPSGRGAGRYIDGAGLKGLRVGGAEISRVHANFIVNVGDATARD
                     VLILMNVARRTVYEKFGVELVPEIRVVGG"
     gene            complement(27612..29048)
                     /gene="murC"
                     /locus_tag="PLU15_00770"
     CDS             complement(27612..29048)
                     /gene="murC"
                     /locus_tag="PLU15_00770"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOO29485.1"
                     /translation="MSSMRVHFVGIGGSGMSPLARILIGHGAEVSGSDIRYSPHLEDL
                     AALGADVYQGHDPSRIDGVDLVVASAAVPQDDPELQAAHERGIRVVSRAELLGEIMRP
                     RRGIAVTGTHGKTTTSGMIAHILNEAGLDPTVAVGAGLPWTPSGGHAGEGEFMVVEAD
                     EAYGSFLKLDPEIAVVTNIDDDHRDYYETFEGIMEGFTQFLRRIKPGGFAVLCADDHN
                     VLRAAEGLDRRIIYYGISSEADYTSADYSQLGFGSRFSVMRRGACLGQIELSVPGMHN
                     MSNAIAAVAVCDQLGIDFSIISRGLAGYMGAERRCQVLAKTPEMTLVDDYAHHPVEIR
                     ATLAALRKAAEGRLVVVFQPQRYTRTKYLRDEFARSFSLADVIAITDIYYEGTGESPI
                     PGVSGRDLAERIAAYEGKPVTYLADRAALDDFLRNSTGPGDTVVTMGAGDIYRSSRRF
                     AEELARPEAGFRLSHDGRWKLDDEHGHR"
     gene            complement(29237..30397)
                     /gene="murG"
                     /locus_tag="PLU15_00775"
     CDS             complement(29237..30397)
                     /gene="murG"
                     /locus_tag="PLU15_00775"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOO29486.1"
                     /translation="MKSEKSAQKRFLLAGGGTGGHIYPAISIARELLSRYPDANILFV
                     GGKRGLEGDLVPKAGFEFQTITVSGFERRLSLDTFATAGRAVAGLFQSIAIVRKFRPS
                     VAVGTGGYVSGPVMLAAAMLGVPVVIHEQNVVPGATNRLLSRWAKTVAVSWEESKQYL
                     ARPERSVLTGNPIRPEIMSAQREESAASFGLDPRRPIVLAFGGSQGARRVNTAFIRAL
                     PSLSGKHDAQFILATGSDKFEESMALAAESGIRASISSDGIGRSDRGDIIIMPYIYNM
                     PQALACADLAICRAGAITLAELAARGVAAVLVPHPHVPDNVQEKNARAMEARGAARVI
                     LDSELEPEVLGRQIDSLLSSRPVLASMSRASRNLGRPDAAARVADCVVKAAR"
     gene            complement(30431..31537)
                     /gene="ftsW"
                     /locus_tag="PLU15_00780"
     CDS             complement(30431..31537)
                     /gene="ftsW"
                     /locus_tag="PLU15_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOO29487.1"
                     /translation="MLNDRGGLPDLVIFVATLALLSIGIVMVFSASSVTAYHELGDPY
                     YYLKRQSLWAIIGIAAMIMCMNIDYHVWERLAGLLMVLSLGALVLVLVPGVGIVVSGS
                     RRWLGVESIRVQPSEFAKLSLVVFLASYLSASQARTKSFFRGFLLPMMIIGACSVLVL
                     KEPDLGTAIGIAGVGWFMLFAGGSNMASLIATVAAAIPAVYLFARQDPVRLRRLTSFL
                     DPWADPLDSGFHIIQSLLALGSGGLVGVGLGLSRQKFHYLPEQHTDFIFAVIGEELGF
                     LGAASVVLLYMIFGLRGFRTSLNAPDSFGSLLAAGITIMVTLQAMMNIAVVSGMMPIT
                     GITLPLISSGGSSLVPMLAGIGILLNVSKHVNQR"
     gene            complement(31558..32523)
                     /gene="mraY"
                     /locus_tag="PLU15_00785"
     CDS             complement(31558..32523)
                     /gene="mraY"
                     /locus_tag="PLU15_00785"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOO29488.1"
                     /translation="MSEVVVRASIAAGIAFAVAMLIGPITIKNLRKLKAGQVVRDDGP
                     QSHLKKSGTPSMGGVIIIVSVLVGSAAASPSYAMLPWALFVTTGCGLVGMLDDYISIV
                     ARRSLGLRARQKILFQLVFGLILGAWAYSNPGIGSGIIIPFTHGHVVDLGMWYIPFVM
                     LVLVASTNAVNLSDGLDGLAAGTMAIASFAYVAVSSALGHGEASAFAGAVGGACLGFI
                     WWNAYPASVFMGDTGSLALGAALASMAVITRTELLLLVIGGIYVVEAMSDVIQVTYFK
                     LTGERVFRMAPIHHHFELKGVAEPKIVVRFWIVAAFLAALGFAAL"
     gene            complement(32523..33929)
                     /gene="murF"
                     /locus_tag="PLU15_00790"
     CDS             complement(32523..33929)
                     /gene="murF"
                     /locus_tag="PLU15_00790"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOO29489.1"
                     /translation="MLDYTLKDVAYAMGGRLAQGDPDQRVSSVVIDSRVAAPDALFFA
                     LPGERVDGHDFLVDAAAGGCCGAVVSRVPVPGQGLPAQFGLIVVDDVLEGLGAFAGRH
                     RMSIDPTVVGVTGSAGKTTTKDMTAAVLAAGFKVIATSGNRNNEIGLPLTLLEMTPEH
                     QIVVVEMAMRGPGEIAALSRIACPDVGIITNIGYAHVGLLGSKENIAAAKAELIEALP
                     PDGFAVLNGDDALVRRMAGLVLGRAWLYGLSEECSVRAESIEPEADSISFSLVIEGHR
                     VDGVRFSVPLPGLHNVYNALAALTVGWRLGLSPDVMATGLASFSPSPMRMEFASSPAG
                     YTVINDAYNANPASVKCAIDAALQYARGRRVILAFGDMLELGETSAVAHEDVGRYAAQ
                     AGVAGMVSVGGMAALAAGAAARSSVEAEACSSNSEALRVLGRMARPGDVVLVKGSRGM
                     QMEEIVGGLLKAPDSGEV"
     gene            complement(33922..35511)
                     /locus_tag="PLU15_00795"
     CDS             complement(33922..35511)
                     /locus_tag="PLU15_00795"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOO29490.1"
                     /translation="MPHNSLESLLQGVSHELSGDPSTEITSLSYDSRKVDGQGTLFAA
                     FVGARFDGHRFIDEAVRRGASAVLVSEPVQVPEGVSVVRVKDTRAALAEIARVFYGDP
                     SSELTAIGVTGTKGKTTTAFMISHILSESGRPSGLIGTMGYMAGKGLEPGPNTTPEAA
                     DVQAILYEMVRAGRTHVAMEASSHGIALGRVALVDWDVGVFTNIGHDHLDFHITIDNY
                     VDAKASLFRSLGKFGAREGKLWPKVAVINIDDPHHCAIFDAAQEAGAEIITYSALGRE
                     GLRGCRHLGAVDVVTGRKGTRYTVHWRESEGPSSKMESVGVCSPLIGAFNVSNALAAI
                     GAVLAVGVPLGEAARALSDFPGVPGRFETVDEGQDFAVVVDYAHTPDSLANVLATARD
                     ISDGRVICVFGCGGDRDRSKRPLMGAIAQRMADYSIVTSDNPRGEDPADIVREIVQGM
                     SEHEAGVEGKAGLEGREKWKIILDRGEAIREAVRCARRGDVVLIAGKGHETYQIFRDE
                     TVHFDDREEARAALRELRMDA"
     gene            complement(35517..37652)
                     /locus_tag="PLU15_00800"
     CDS             complement(35517..37652)
                     /locus_tag="PLU15_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO29491.1"
                     /translation="MRSRTSSRTGYRADATSAIIRKRIYSVLLLMAGLFVVVLGRLVW
                     VQIVKAPYFRELALEQRMRPVPVDPKRGTMYDRCMRELAVSISADSVYAIPAEVSEPA
                     KTAKALAHVLGMGEDALLAKLTATQQTVWLKRKVDHEAAMEVRRLSLPGIAVAESGQR
                     FYPKDTLAAHVIGIAGIDNQGLEGLEQYYDAELRGVRGQIVAERDATGREIPEGRHSY
                     VPPTDGNSLVLTIDEVIQYICERELEIAIAETGSTRGLILAMDPKTGEILAMAMRPTF
                     NPNSYGKYPSEFRRNFIVSDMLEPGSTFKVITAAAALEEGIVTASTPFYDPGYLKVED
                     RTIRCHKPGGHGSETFMEALENSCNPVFATLALRLGVEKFVQYARAFGLLEKTGVDYP
                     GEATGMMHQISKVGPVELATYGFGQGISVTPLQLLNAMCAIANGGALMRPMLVRKVIG
                     ADGALVEEHVPTKVRQVISSSTASELSRMLQSVIVNGSGQRGAVPGYAVAGKTGTAQK
                     PERGRYGDKRIASFMGYGPASDPKVAVLVILDEPSSDVKYGGVIAAPVFRAVMQDIFR
                     YMEIPPSYAVGMPQGTAGSGNSVFVPDVTGKSRAEAEAGLKEVGLLWRHEGIGDVVTN
                     QVPKAGSQVGAGTTVIVYFGHEELYNREDVEIQVPDVIGKTLKEAALVLGARGLGVDP
                     SGKGLVVRQTPAPGTHVKKGAIVTIFLEE"
     gene            complement(37680..38198)
                     /locus_tag="PLU15_00805"
     CDS             complement(37680..38198)
                     /locus_tag="PLU15_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOO29492.1"
                     /translation="MECTNARLGDAGRESVRRRNTRSFRLMAECVIVAVLAIVLLVMS
                     IAQRTEIMRACDRRSDVQARLDLLKNEWDRKSLELARVASLERVDAIARTELGMASPD
                     NMGIVVVDARPMLAEAEVAPVAAGTQGLSEPPTVLAGVATIMQQMAVAAVSNLIAGWF
                     AAPPAQLPSVLR"
     gene            complement(38201..39157)
                     /gene="rsmH"
                     /locus_tag="PLU15_00810"
     CDS             complement(38201..39157)
                     /gene="rsmH"
                     /locus_tag="PLU15_00810"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOO29493.1"
                     /translation="MSYTHRPVMLSEVLEYLDPSPGDVICDATCGGGGHSLDIARKIG
                     ESGVLIGIDLDPQALNAARQNLEGVPCRVILARGNYGDMAVILEEAGYPNPNGILFDF
                     GVSSHQLDTAERGFTYRAEAPLDMRMDPDGPVTAADILNTASEGELERIIREYGEERW
                     ASRIAEFIVERRKTAPLETSGDLVNLIYAAVPRGARRDGPHPARRTFQALRIAVNDEL
                     GSVERGILAAIDRVQPGGRIVAISYHSLEDRVVKEAFKRAERPCTCPPSLPVCVCGKK
                     KVLDVLTKKPIGPTDEEVRSNSRSRSAKLRAARKVLGTLGGE"
     gene            complement(39170..39610)
                     /gene="mraZ"
                     /locus_tag="PLU15_00815"
     CDS             complement(39170..39610)
                     /gene="mraZ"
                     /locus_tag="PLU15_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017687291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOO29494.1"
                     /translation="MFIGEHRHCLDDKGRVIIPAKFREKLDGRFILTRGLDVCLFAFT
                     MDEWRLQEEKLSALPMTMADGRAFSRLFFSGASECELDKQGRALVPAHLREYARIERD
                     IVIIGVSSRFEIWAAEVWEEYLSRVSTAYESIAEKLVSYGPNQQ"
     gene            complement(39773..40306)
                     /locus_tag="PLU15_00820"
     CDS             complement(39773..40306)
                     /locus_tag="PLU15_00820"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO29495.1"
                     /translation="MQQLERAVLRPLEERDLDRIAGWDSDSELAHMMGGVPISIEESR
                     GRYLKMLNDRNSLVMAIVDHSGRLIGDIELTEIAWRSGDAELSIKIGDPAYRDRGIGE
                     CAVREMLFIAFERLGLRRVYLRVCSDNLRAVRCYKKCGFRREGVVKRRLGPDDQVRVV
                     TLMTILRSDFMNMQAVS"
     gene            complement(40515..40775)
                     /locus_tag="PLU15_00825"
     CDS             complement(40515..40775)
                     /locus_tag="PLU15_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOO29496.1"
                     /translation="MEVLKVSAKSNPNSVAGALAGVIRERGGAEVQAIGAGAINQAVK
                     AVAIARGFVAPSGLDLVCIPAFVDVTIDGEERTAIKFIVQPR"
     gene            complement(40870..41661)
                     /locus_tag="PLU15_00830"
     CDS             complement(40870..41661)
                     /locus_tag="PLU15_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOO29497.1"
                     /translation="MRVAMLGDIIGRPGRRHTLRWIADLRSREELDFVVVNGENAAGG
                     IGLTPDVSLEIFDAGVDVITTGNHVWRNREMAGFLQTEKRVLRPANYPQGVPGNGWTI
                     VTKGALRLAVLNLQGRVFMQPIDCPFICADRAIEQIGSNADAIVVDFHAEATSEKQAL
                     GLYLDGRVSAVVGTHTHVQTSDERVLANGTGFITDLGMCGPVNSVIGMDPDTVIPKFI
                     TAMPSKFEVGKGPVIVCGVIVEIDTSTGKTKYVQRFLEELDDTIR"
     gene            complement(41764..42315)
                     /locus_tag="PLU15_00835"
     CDS             complement(41764..42315)
                     /locus_tag="PLU15_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29498.1"
                     /translation="MKKKVIAVKEVRTKEIDGSSASIGLLVSILVRYPEVCTVSYTPD
                     AKMLGFSFMVSRRLEDDEVEGFRSSVLDSISVYLDLLKFGETPTVEVCAVYLGSITQI
                     QVTRDVATLTGDEISLLIGLIIDAFDDDLVTDRSEDLEEEEMIVQEEFIANLLVDLKE
                     TTQEKRLIGFREGGRVIVYNRPA"
     gene            complement(42401..43930)
                     /gene="rny"
                     /locus_tag="PLU15_00840"
     CDS             complement(42401..43930)
                     /gene="rny"
                     /locus_tag="PLU15_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOO29499.1"
                     /translation="MQALIYVAVVALSAYLGYLARKKIAEAKISVAEDAAKNIIEEAE
                     REAEAKKREAVIEAKEEVHRMRSEFDRECRDRRNELQRVERRILQKEESLDRKSDQLE
                     RKESQIDRREKEIDRTQAEIEKIHQEQRQELERIAGLTTDQAREQLLREAEEEITHDV
                     AVMIKEAESQAREEAERRAREIVTQAIQRCAADQVAEGAVSVVALPNDEMKGRIIGRE
                     GRNIRALETLTGIDLIIDDTPEAVILSGFDPVRREVARIALEKLIQDGRIHPARIEEM
                     VERAQKEVDAVIREEGERATFEVGVHSIHPELVKLLGRLKYRTSYGQNVLQHSIEVSH
                     LAGIMASEIGADVAMAKRAGLLHDIGKATDHEMEGPHVTIGADLARKYRESPAVVNAI
                     AAHHGDEEPQTVEAVLVQAADAVSAARPGARRETLEAYIKRLEKLEQIADSFEGVDKA
                     YAIQAGREIRIMVKPDKIDDLASVKLARDIVKKIEQELEYPGQIKVTVIRETRSVDYA
                     K"
     gene            complement(44157..44774)
                     /locus_tag="PLU15_00845"
     CDS             complement(44157..44774)
                     /locus_tag="PLU15_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOO29500.1"
                     /translation="MNGGSGRLDLPGVSGQSGKPGDEHSPVARSSGQSLPWHSDSDVN
                     ASCSRAHSDARAQAYKILSVRARTEKELRDALSKKGHDSAVVDDIVGEMIRCGYIDDV
                     DAAHRWAAMCVDERRHGAKGIAMRLVRRGIDPDLADEAARSAYSAAGLEEFEVALELA
                     EKRVKDDDLCDDKARLRSQRRVAGFLARRGFRSDTIARVVRELFR"
     gene            complement(44774..45892)
                     /gene="recA"
                     /locus_tag="PLU15_00850"
     CDS             complement(44774..45892)
                     /gene="recA"
                     /locus_tag="PLU15_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOO29501.1"
                     /translation="MRKGRWDLSDREKALEVALTQIEKDFGKGSIMRLGESGGVMQVD
                     AIPTGAIALDIALGVGGMPRGRVAEIYGPESSGKTTVALHVIAEAQKLGGIAAFIDAE
                     HALDATYAKRLGVDIDNLLVSQPDTGEQALEITEALVRSGAVDVIVVDSVAALVPRSE
                     IEGEMGDSFVGLQARLMSQALRKLTGAISKSNTCVIFINQIREKVGIMYGNPEVTPGG
                     RALKFYSSVRLEIRRVESLKQGSEQIGNRTRGKVVKNKVAPPFKEAEFDIIYGEGISK
                     EGSLLDVGEAQGIIIRSGTWYSYGDLRLGQGKENCRQFLKSNPELAAEIEVKVRESVG
                     LPLAGSPASGSGAASDAKDGGKDADGSGKKDGQRKSKS"
     gene            complement(46058..46624)
                     /gene="thpR"
                     /locus_tag="PLU15_00855"
     CDS             complement(46058..46624)
                     /gene="thpR"
                     /locus_tag="PLU15_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOO29502.1"
                     /translation="MDTVRTFIAVPVAGPAAHSVADLIAEMRKVGASVKWVETRNLHL
                     TLKFLGDLPLSRIPALAGAVRKAVEGDAKFEMTLQGAGAFPDVARARTLWVGVDRGAQ
                     RLAGLAEAVDRATVDAGFAPADKAFRPHLTIGRARERSAGAGISRIISENADIYLGAV
                     DVKAIHICSSKLTPSGPIYTSLAEIPLE"
     gene            complement(46749..48017)
                     /locus_tag="PLU15_00860"
     CDS             complement(46749..48017)
                     /locus_tag="PLU15_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOO29503.1"
                     /translation="MKAEIISVGTELLLGQTVDTNAAHIARSLAGWGFSLYRKTTVGD
                     NRARLATVVREALERADVVITSGGLGPTEDDLTKETVAEVLGIHLVESPEVLSDIEGF
                     FASRGTRMTANNRKQALVPEPSSGTFFRNAVGTAPAVVFESGKKRVICLPGVPREMAY
                     LINSGVEPYLRAYLTRNGAGEVIVSRIIRTIGIGEASLEERILDLACEGVNPTVAVYA
                     SEGECQIRVTACAREEKEAWMLIEPVEKELEARLGRSIYGYDDDTLEGVVARRLISAG
                     ARLSVAESCTGGLICGMLTGVPGSSSFLERGIVTYSNSAKRDELGVDESTLRVHGAVS
                     RETAVEMACGVVRTARADLGLSVTGIAGPGGGSADKPVGLVYFCLADTEKTSVITARM
                     MFGGDRRSVRTRACKFGLDLLRRHLEDAWT"
     gene            complement(48096..49586)
                     /locus_tag="PLU15_00865"
     CDS             complement(48096..49586)
                     /locus_tag="PLU15_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO29504.1"
                     /translation="MIRDVLIGTGIAAFLFCIVYGYDVTPIIFAGGLYAAFELMMSGR
                     VGDRKFEVIQEAESGGRVSKLTFGDVGGQETAKRELVEALEFINNEEKAVKLGIRPLR
                     GILLSGPPGTGKTLLAKAAANYTDAVFISVAGSDFIEMYAGVGAKRVRDLFGTARSTA
                     RACKKKTAVIFIDEIDVLGGVRGTHQGHLEYDQTLNQLLVEMDGLTSASREGVRVLVI
                     GATNRADLLDPALLRPGRFDRLVDVDLPDREGRRHILEIHTRHKPLAEDVDIDKVAAE
                     TYGFSGAHLESLTNEAAIMALRAGRVRITMNDFSEAMEKVIMGERIERKPDEEELRRV
                     AVHELGHAVVSELLKPGSVAAVTVSSRGKALGYVRHAPRSDKYIETAAELEARISVLL
                     AGAMAEELILGERSTGASQDIQQAVETARKIVNCGLSDLGIISEEDIPRDQYHTAVTR
                     LVQAQEERAKSLLVPNAEFIRKAAGELMSVEKMNGDTFRAQLSQTA"
     gene            complement(49583..50185)
                     /locus_tag="PLU15_00870"
     CDS             complement(49583..50185)
                     /locus_tag="PLU15_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29505.1"
                     /translation="MKLPGINIKVVLASVAVAAVLLFGGREVFLRTSVAMPMDRAFAS
                     MSEIESYSFERSAYGAVVEVKLGNVEDLSETVRKVEAAVAKLPADGRLWVRISDTRDV
                     LLQDVYYRMHFDIEEAIATGSFSDLKGRADEKARQAGLSGCVVQVDADNVYVSLENGE
                     HRLYAVVSRASGAVQGGTVVRSDARWLSTCSPRGKAGVGR"
     gene            complement(50339..51028)
                     /gene="rpe"
                     /locus_tag="PLU15_00875"
     CDS             complement(50339..51028)
                     /gene="rpe"
                     /locus_tag="PLU15_00875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOO29506.1"
                     /translation="MIKIAPSILSADFGILAEEIRGIANDADMIHIDVMDGSFVPNIT
                     LGQPVVRRIRQATDLPFDVHLMIVNPEEHIDSFADAGADAITVHAEASRHLHRTLEGI
                     RQRGVRAGVALNPATPVCAIEHVLHMVDMVLVMTVNPGFGGQSMIVEALNKVEAIAAL
                     ARRNGLSFDIEVDGGINDETIGAAARAGANVFVAGSYVFGAENPREAIGVLRAAAIAA
                     AESSQRTQQMI"
     gene            complement(51163..52050)
                     /gene="rsgA"
                     /locus_tag="PLU15_00880"
     CDS             complement(51163..52050)
                     /gene="rsgA"
                     /locus_tag="PLU15_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOO29507.1"
                     /translation="MYDIGRIVRISGGLCWVLPDGEQETVPCAARGNVKRREEGVAVG
                     DRVRMLRQNGTGVVEEVLPRRTYLVRPTVANVDQAIVVAAAAQPDPNPCLLDRLLVMS
                     EYAGLDAVVCINKADLVTRDECESLLRLYSDIGYKTILASARTGLGLEALSAALAGKT
                     SVFAGPSGVGKSALLNMVQPGHDLVEGEISEKIGRGRHTTREVRLIPLDVGGFVADTP
                     GFSSLDITSIAPRELTHVFPEISRLASECKFVGCLHDKEPDCAVKEAVERGVVDPGRY
                     AHYLSFLEELREAEKRQYR"
     gene            complement(52043..54040)
                     /gene="pknB"
                     /locus_tag="PLU15_00885"
     CDS             complement(52043..54040)
                     /gene="pknB"
                     /locus_tag="PLU15_00885"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOO29508.1"
                     /translation="MTGEILGGRYSILDTIGEGGMAIVYRAKDMLLNRVVAIKVLRGQ
                     YASDSEFRERFRREAQSAAALSHPNIVNVYDVGEDSGSNYIVMEWVDGQTLNDIIMRD
                     GRLTVDVVADYSVQILDALDHAHRSGVIHRDIKPHNILITRDGRVKVTDFGIARAASA
                     SALTEAGQVIGTVNYTSPEQARGASAIAESDIYSLGVVMYEMLTGRLPFVGDTPVAVA
                     LKHVQEDALPLSVYNKAVPVEFERVVMKALAKDPQDRWHTARAFKNAILRAHPTEVKK
                     VPDGQQVEDLPTASGSEGAATPHSRITNGVDDDLNITRPFDKVGEAFELENDQETPKR
                     ADRQAKTWTTIIVVMLVVAGAVLGALYSFRQWIQVEEVVVPDVVNMTLPDAESLARRS
                     RLALDSSVKRYDNKVEYNRIIEQDPPAGAKRKVHSPIKVIVSLGPEMVTVPDLFNKSE
                     REARLELERSNLVLGAKSQDYHEDVRPGRIVDQEPEVGTYVPRGSTVNYTVSIGPPPP
                     PPEVPLVIGMTLERATDTLKRAGFAVGEVRKRISILYEDGTVIEQNPGPYQMAESGTT
                     IDLVVAQRSGFPSASPSPSDDGGGQIDMSYRRYDVTYQVQPGSEVQEIVIKVNDSYGE
                     RTSYGPKLHRPGDWIRHTVEVYGPGKIEVWVDGYPVKTENV"
     gene            complement(54045..55451)
                     /locus_tag="PLU15_00890"
     CDS             complement(54045..55451)
                     /locus_tag="PLU15_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO29509.1"
                     /translation="MNRRIRRLVQAWIAGCAILVVAATYYQAISADKLSSSQANPRLA
                     NLERDVIRGPILDRNGGVICDSVGPGLSTRVWRGPDSLASLVGYVNPVLGKSGLEAAY
                     DVWLSGRRGPYDVTGLLATLAGQPDHGCAVRLTIDANVQKACESALRGRRGAAIVINA
                     GTGELLAVASSPWYSMKGLELSWDNIVSRNDSPLVCRPINGMYPPGSTIKMAIAAIAL
                     DSGVTDPNEVYECEGSIRAGGSVVHDPTGAHGAIDIVDALAVSCNTTFVDLGLRLGPK
                     LYEELRRFFFDRSVPLDIPTPPANLDVRNADVSGDAIGQLAIGQGNLLVTPMHVAMMA
                     AAVANRGIMMRPYVVADISAVDGVPVMVASERALASVMTPSSADIVARGMRQAVKRGT
                     ARSAARWGYDIAGKTGTAQNPHGNPHAWFAGFGVNGNNRVAAAIILENAGSGGTAAAP
                     LGGDIIRVALDSMDRARR"
     gene            complement(55448..56767)
                     /locus_tag="PLU15_00895"
     CDS             complement(55448..56767)
                     /locus_tag="PLU15_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOO29510.1"
                     /translation="MMEDGSLPASAQGTARPIRETERSLLGIVMLMSLTAGFSLSLAR
                     NGTLTGEEAALCFVSSAPFAMTHLVFVAKGFDGDELILPLAGAATVMGYPVVYRLRPD
                     LAVRQLAFIWLGSAVMAVLAILQNQKKLEGWGVPALGGSIALLVLTLMVGEERGGARS
                     WLFLGDMGFQTSELAKITCILAGAEGLSKMHTADSMATARASAREIWECSQARRVIVM
                     WAAVMLLTVAQRDVGTAMVIYISCVAMAYVATGNRLLVLVSIGIGAAAVAVAGRVFPH
                     VGVRFSTWLNPWADAAGSGYQVTQSIYALASGGILGRGYGCGSPWLIPAAVTDMLFSA
                     SAEEIGLAGSIGLIAIYFLMVGHGYACAQRADSRYSALVAVGAANMIAVQTFVIIGGN
                     LGVLPLTGVTLPLASYGGSSMLTSLAIVGILLGSTRRLHSIDGGALS"
     gene            complement(56764..57570)
                     /locus_tag="PLU15_00900"
     CDS             complement(56764..57570)
                     /locus_tag="PLU15_00900"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOO29511.1"
                     /translation="MMRLEFVYASLDGVRDVMVGFATDIGLVRDRNEDAYFAADGCYA
                     VADGMGGHRAGEVASEIAIGVVSAECANVDLGSLDMAQCIEKANRSVIEASRRCDELE
                     GMGTTLTVAAFCEGTLRVGHVGDSRAYLIRGDDIIQLTDDHSVVGELLRSGMLTPEEA
                     ECHPQRHAITRALGFSESLEVDIVERQVEPGDVVLLCTDGLHDIVGDNEIAETIKRFH
                     DPREACMELVRLARARGGHDNITVVLVRVEESDVSSSSSSARACSGGLVI"
     gene            complement(57557..57748)
                     /locus_tag="PLU15_00905"
     CDS             complement(57557..57748)
                     /locus_tag="PLU15_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29512.1"
                     /translation="MVRVALAILRLACVASLYFFLYRVCRALVLDVSFDAGSDNLDDE
                     KMLEGQSAAVPDAGGDDAS"
     gene            complement(57756..58847)
                     /gene="rlmN"
                     /locus_tag="PLU15_00910"
     CDS             complement(57756..58847)
                     /gene="rlmN"
                     /locus_tag="PLU15_00910"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOO29513.1"
                     /translation="MIKTVDIKNMSFEECRGFFASLGEPAYRADQVFEWLHGKRTASY
                     DEMTNLPVSLRRTLAEVAPLRPPEVLAVHSSPGGATKYLIGLADGNAVETVLMEHDYG
                     LSLCVSSQVGCGMGCAFCASGLEGLVRNLTCGEMIDQILAVSNGIANESPNRRVSSVV
                     MMGSGEPLANYDNVLKFVRLINWKRGYGIGYRHITISTCGIVPGIRRLAQEGLPVTLS
                     VSLHAPDDHTRSALMKINQVYPVGQLIEACIDYANSTGRRVTYEYSLIGGVNDSRDHA
                     LRLARLLGGTLCHVNLIPLNPVPETGFARPSQEAVNVFACTLRSAGIETTIRREMGGD
                     IDAACGQLRRRWAGRPGASDPSKRRDEGG"
     gene            complement(59166..60107)
                     /locus_tag="PLU15_00915"
     CDS             complement(59166..60107)
                     /locus_tag="PLU15_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29514.1"
                     /translation="MEFLYFVADALATTVSLAAPLILTAIGGVFSERSGVVNIGLEGM
                     MLIGSFMGVLFSHLTGNAWIGFWMGALSGAAVAALHAWVCIKHGANQTVSGVAINILG
                     AALTGYLLRAIFNRAGQTEPVPGLAAWTIPFLKDVPILGDIFYRNMPPVYIAFIVVIV
                     AQIVLFKTPLGLRIRACGEHPEAADTVGVNVAGIRYLCVILSGFLAGMGGVTLSLGQL
                     SLFREGMSAGKGFIALAAVIFGRWTPVGAMWASLLFGLADGLQLTAQNWGFTAIPREF
                     LIMTPYIVTMAALAGIIGRSVPPAASGKPYVRRDGGA"
     gene            complement(60109..61206)
                     /locus_tag="PLU15_00920"
     CDS             complement(60109..61206)
                     /locus_tag="PLU15_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29515.1"
                     /translation="MSVESKRKAWWRQLIVPIIAVVLGLLIGAIFMLFTGANPLDAYG
                     ALLSGCFGSAVNLSETVVYITPLICTGLSIAVAFRAGLFNIGAEGQMIVGMLASAIIG
                     YKLAGLPRIIHLPLTIFFGMLAGALWAAVPGYLKAKFGVHEVVNSIMMNYIALYLSHY
                     LVNGPIKDPNLASPYSPEIADSAKLWRFVGGHGSEFRVNSGILIAIAAAILIYRLLYK
                     TTRGYEIRAVGLNAEAARYAGIDVSSAMARSMLIAGSMAGLAGAIQVCGIQYKFLDLF
                     AFEGFGFDGIAVALLAQGNPYGILAAASLFGVLNRGSQMMQLLARVPKETAGIIQASV
                     VFLVAAEGIVEGWLPVRRTASLEASGKEETQ"
     gene            complement(61203..62765)
                     /locus_tag="PLU15_00925"
     CDS             complement(61203..62765)
                     /locus_tag="PLU15_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29516.1"
                     /translation="MGRPQSVPVLEMRGITKQFPGVVANDNVDFKLEKGEIHALLGEN
                     GAGKTTLMKILYGLYEADEGMVLVNGKEISIKEPNDAISLGIGMVHQHFMLIPPFTVA
                     ENLVLGAEPVKNGLFMDMEKAIAKANEVSEAYGLAVDPNAKVVDISVGMQQRVEILKA
                     LLRGAEILVLDEPTAVLTPQEVTELFEIMRNLTEQGKSIIFISHKLKEVLDISDHVTV
                     LRRGKSIGTVATAETTQQELARMMVGRDVELVVEKGPAKPGKNILQVSELHVLDQRHL
                     PAVRGVSFSVRAGEIFGIAGVDGNGQSELVEAITGMRRPTSGRVVINGHDLTGARPRQ
                     VFESGVVHIPSDRHRHGLVLDFTIAENMILQTYYKPPFSKRGELDWETIHQEARRLIE
                     EFDVRTPSEKVAAGSLSGGNQQKVIVARELSRQPELIVAAQPTRGLDVGAIEFVHRKL
                     VEARDDGKAVLLVSLELDEILALADRIAVMYEGEIVGVVDSDTVTEEELGLMMAGAMR
                     GPKAETAGGGGR"
     gene            complement(62952..63974)
                     /locus_tag="PLU15_00930"
     CDS             complement(62952..63974)
                     /locus_tag="PLU15_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO29517.1"
                     /translation="MSSLKRVLVVFLAAGMLVGCAFNGFAAPAPKVGIVFDIGGLGDQ
                     SFNDSANVGLERLKKELGYGTRYVESNTPSDYEPNLASLAREGYDVVWGIGFLMADAV
                     QKVAKQFPNTKFGIIDHGYSDAEYKEIPNVLGILYKEHEGSFLVGVLAALTTKTNTVG
                     FVGGMDIPVIHRFEAGYKAGVWKTNPNINIIVNYSGAFDDPGKGKTIAESMFRRNADV
                     VFHAAGSTGIGVIRAAEDHGKWAIGVDSDQNHLAPNNVLNSMIKRVDVGVFEGTKMLL
                     KPGFTGRTVVLGLRENAVGLAPEKSNRASAAATAKAREWANIIARGEYVVPEFPAEAD
                     KLYKKK"
     gene            64501..65751
                     /locus_tag="PLU15_00935"
     CDS             64501..65751
                     /locus_tag="PLU15_00935"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOO29518.1"
                     /translation="MAGGQYEQVLARLREAARILNLEPGIFEILAEPERTLEVALPIE
                     MDDGSIKVFTGYRCQHSTVRGPAKGGVRYAMDVCMDEVKALSSWMTWKCAVAGIPYGG
                     GKGGIRVDPTQLSENELKRLTRRYVYAIYPIIGPEKDIPAPDMGTNAQIMGWFVDTYS
                     VLNGVYSPGVVTGKPVPIGGSIGRREATGRGVVFAVRELLLRLNKKPSEITCAVQGYG
                     NVGSVAATLAHEMGCKVVAVSDISGGHYNPGGLNIPHITKYVEENRVLEGYSEPGVSN
                     ISNADLLTLDVDLLIPAALQNQITEENAEDVRAKFIVEGANGPTTAEADKILAANGVL
                     VVPDILANAGGVICSYFEWVQNLQNFYWTEEEVNSRLDKIMTHSFAEVWDFAHEEDLS
                     LRTGAYILGVKRVADAIKMRGIFP"
     gene            complement(66309..66845)
                     /locus_tag="PLU15_00940"
     CDS             complement(66309..66845)
                     /locus_tag="PLU15_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO29519.1"
                     /translation="MLEELIISGFGGQGVMVIGQLLSYAGLLEGKNVSWIPSYGPEMR
                     GGTANCSVVISTDPIGSPIVSEPLSVIVMNRPSLDRFESALRPGGLLVYNSSLIDRAP
                     IRTDVRVIAVPVNDMAAELGSDRVGNMCALGAYLAATRVVSIDSIAESLKEVLPPRRH
                     DLIPLNLKALEAGRELIK"
     gene            complement(66845..67594)
                     /locus_tag="PLU15_00945"
     CDS             complement(66845..67594)
                     /locus_tag="PLU15_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO29520.1"
                     /translation="MKQVYSVPKSLQPRSTHYCPGCTHGIVHKLVAEAIDDLEIRDRA
                     IGVAPVGCAVFAYDYFDCDFQQAAHGRAPAVATGIKRAVPDSVVFTYQGDGDLASIGA
                     AEIVHAAMRGERITVIFVNNAIYGMTGGQMAPTTLVGQKATTAPFGRQKEHCGSPLRI
                     SEMLATIPGAAYIARVALSSPGNVAKAKTAIRKAFETQLAGEGFALVECLSTCPTNWG
                     MAPYEAMQWLEENMIPYYPLGEFRTPEGGAE"
     gene            complement(67591..68661)
                     /locus_tag="PLU15_00950"
     CDS             complement(67591..68661)
                     /locus_tag="PLU15_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOO29521.1"
                     /translation="MVERKLFKGNEAVAEAAVRAGCRYFFGYPITPQNEIPEYMARRL
                     PEVGGLFLQAESEVAAINMVYGAAGTGARVMTSSSSPGISLKQEGISYIAGAELPCLI
                     VNMVRGGPGLGGIQPAQSDYFQAVKGGGHGDYYTIVYAPASIQEMVDLTMQAFDVAET
                     YRMPVMILGDGVLGQMMEPVEFRDPVPRELPPKDWATTGAKDGRAPRLINSIYLKPDE
                     LFKHNLKIAERFEQAKKNEVMCEKANTDDADIVLVAYGTTARVCRSVMDRARAAGLKV
                     GLIRPITVSPFPYDDIAEVARTAKAFLTVEMSLGQMVEDVKVAVYGAAPVHFYGTTGG
                     VIPTAADIMARVEEIARAEVRK"
     gene            complement(68676..68882)
                     /locus_tag="PLU15_00955"
     CDS             complement(68676..68882)
                     /locus_tag="PLU15_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO29522.1"
                     /translation="MTKVVFDEEKCKGCGLCVQFCPKKAIIMAERINTKGYHPAVLAD
                     EENCISCGICARMCPDAVIEVFKP"
     gene            complement(68879..69967)
                     /gene="buk"
                     /locus_tag="PLU15_00960"
     CDS             complement(68879..69967)
                     /gene="buk"
                     /locus_tag="PLU15_00960"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOO29523.1"
                     /translation="MSDFRVLVINPGSTSTKVAVFEGDRSAASESLRHDADMIASYPR
                     IFDQFDMRKEAVFDFMERLPFPADTLSAVVGRGGVLPPMEGGVYIVDDVLVDALYEWG
                     WNHASSLGGIIAKEIADSLGIPSYIADPTTTDELDNIARMTGIPGIERRSVFHALNQK
                     AMARKAARDLGRKYEECNFIVVHLGGGISIGGHKRGRVVEVSNALDGEGPYSSERSGS
                     LPNTSLVRLCFSGRYTEAEMRRKITGQGGLVAYLGTNSLLDVEKKIEEGDDYARICYE
                     GMAYQVAKEIGRVACALSGDVDAIILTGGAANSKMLVSWIEERVKFIASVMVYPGEAE
                     MEALADAGLRVLRGLEEPKRMMLGGGGR"
     gene            complement(69985..70881)
                     /gene="ptb"
                     /locus_tag="PLU15_00965"
     CDS             complement(69985..70881)
                     /gene="ptb"
                     /locus_tag="PLU15_00965"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HOO29524.1"
                     /translation="MLAAFDEVVARAKKRPSAKVAVAVAQDREVLEAIQMAHGIGMGA
                     FVLVGDEKLIRPLASAVGLDLSELEIVHEPDARTAALHAVELVSSGQAGILMKGLIPT
                     ADFLRAVLDKEVGLRTGKLLSHVAVFKSPRYDRLIYMTDGAMVVAPTLQDKVQIIQNA
                     VGVAHKLDNEVPKVACIAAVEVVNPDMPETVEAAALAKMCDRNQIRGCIVDGPLGLDN
                     AVSEESARHKGVDGPVAGRADILLVPNIVAGNVIYKTLVYFGQVETAAILTGARAPIV
                     LTSRSDSPEARLKSLALAIATS"
     gene            complement(70929..72017)
                     /gene="buk"
                     /locus_tag="PLU15_00970"
     CDS             complement(70929..72017)
                     /gene="buk"
                     /locus_tag="PLU15_00970"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOO29525.1"
                     /translation="MGCIDRTFTVLVVNTGSTSTKIAVFCGCKLLFSDNIQHPPDELA
                     RYARIPEQLEYRKGVIQRSLASSGVALEQIDAIISRGGALKPVPGGVYLIDEAVVDDL
                     LNRPSTEHAANLAAPIARAFADSLGVPAYFADPISVDEYEPLARLSGLPEISRVSKLH
                     ALSIRSTAAKAAASLGKALEEANLVVAHLGSGISISPVKAGKMVDANGADDEGPFSPE
                     RSGSVPMSDLVTLCYSGQYDEGELIHLLTHGSGLFAHLGTTDAREVEKMILAGNTRAS
                     DVEQAMAYQISKEIGAMATVLCGRVDAIVLTGGLAAWDRLVGHITERCCFIAPVITYP
                     GENEMEALASSVMRVLAGEEKARVYASV"
     gene            complement(72172..74364)
                     /gene="pnp"
                     /locus_tag="PLU15_00975"
     CDS             complement(72172..74364)
                     /gene="pnp"
                     /locus_tag="PLU15_00975"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOO29526.1"
                     /translation="MHQVFQADIAGKRLAVETGYVAQQANGSVLCRYGDTVVLVTATA
                     SKEPREGIDFFPLLVDYEEKLYAVGKIPGGFIKREGRPGEAAILAARLIDRPIRPLFP
                     DGFRNDVQVVATVFSVEPGIDPALLAMNGASIALSISDIPFDGPIAGVKVGLVDGQFI
                     INPGVDESERSTLSLTVAGTEDAVLMVEAGANEVPEEQVLDAIIYGHETIKEIIQFQK
                     KIVAEVGVSKKEFVAFTPDPEIAEKVEEYAYDKLNQAVRNPDKVAREELTHAVREEVL
                     AHFAEIYGEEVPVRDINGVLDVLLKQIVRRMITQEQTRPDGRALDEIRKITCDVGLLP
                     RVHGSAMFRRGQTQVITACTLGAIGEEQILDDLGLEESKRYMHHYNFPPYSVGEVKPM
                     RGPGRREIGHGALAERALLPVIPDDEEFPYTIRVVSEVVESNGSSSMASVCGSTLSLM
                     DAGVPIKKPVAGVAMGLVKYGDDFAILTDIQGIEDALGDMDFKVAGTRDGITAVQMDI
                     KVRGISREILQAALEQARRGRLFIMDRMLETIPQPRAELSPYAPRMLTINIDPDKIRD
                     VIGPGGKMIRKIIDLTGTKIDIEDDGRVFVSAVDEAQAQKAINMILELVHDPEPGEVY
                     TGKVTRIMPFGAFVEILPGKEGLVHISQLAHERVATVEDVVQVGDDINVRVTEIDSQG
                     RINLSRKDLLPRPEGMPDSPPPRGDRRPPSGTRGGGAPRGRSGGRDRR"
     gene            complement(74450..74719)
                     /gene="rpsO"
                     /locus_tag="PLU15_00980"
     CDS             complement(74450..74719)
                     /gene="rpsO"
                     /locus_tag="PLU15_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOO29527.1"
                     /translation="MSLTTEEKKSIIEKYQLHESDTGSPEVQIAILTERINYLTEHLK
                     VHKKDHHSRRGLLKMVGKRRALLDYLKDSDIERYRALIEELGLRK"
     gene            complement(74829..75803)
                     /locus_tag="PLU15_00985"
     CDS             complement(74829..75803)
                     /locus_tag="PLU15_00985"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOO29528.1"
                     /translation="MERFFISTSERPPVTAQVFALGTFDGVHLGHMELIREAISLARD
                     KGEGPRSGVYTFDVHPLAVLDPSKAPPLLTSLDDRLKLIESAGIDVAVVQSFDAELAD
                     MSPESFVADILWRKLRARHVVVGFNYTFGRYGNAGASELEEMCARYGIGTTVVPPVTC
                     EGITVASTAVRTALAEGDVVLASRLLGRPYFLRGIVERGRGVGKSIGVPTANLRLPNG
                     HAIPAHGVYAARASWGDGQCAMAVCNIGVRPTFRDEGRGTTCEVHLVDVSQALYGEML
                     MVEFVARIRDEKRFASPSHLKAQIASDVEATRALIGNWSLHPISSVLE"
     gene            complement(75824..76747)
                     /gene="truB"
                     /locus_tag="PLU15_00990"
     CDS             complement(75824..76747)
                     /gene="truB"
                     /locus_tag="PLU15_00990"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOO29529.1"
                     /translation="MDGIIIINKPKGMTSHDVVVEVRRITGERSCGHTGTLDPDATGV
                     LVVCMGRATRLSRFMMETPKEYEAVIVFGISTDTGDASGKVVAVSEDVKVPRERVFEA
                     FQRFVGDIEQIPPMVSAVRHRGRRLYELAREGITVEREPRRVTIYEIEPASLEDWPEV
                     VRLGSQGSMRVVCSKGTYIRTLCEDICASIGIEGHMGALERTASSGFRLSEACTLNEL
                     RQAAAYGEFDNLVRPIVEGVGHMPKCVVSEDEASRIKHGTQIEWDPNRAEPVCGIGDY
                     IALLGADGALVAVGEVRLMDGRALIQPRVVL"
     gene            complement(76735..77721)
                     /locus_tag="PLU15_00995"
     CDS             complement(76735..77721)
                     /locus_tag="PLU15_00995"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOO29530.1"
                     /translation="MSAPEELLQAIVDAKSILITTHVAPDGDAIGSALALKIALDRMG
                     KGPRVGLSDPVPEFLRFLPEADSIETAECVGGPFDLALVLDCGSLDRVGKCENAVVSS
                     RKVANVDHHTTNTHFADINWVDSEAAATAQMAAGIIRDLGVEFDPDIAACLFAGLSTD
                     TGSFRYSNTSADTFRVAAALVDAGARPWEIAQNIYDTKPYKSLSALAEAILGIQFSSG
                     GLIASMEITAEMMERHGITESETEGFIGYARSIEGVEVAVAFREVEDGLIRIGFRSKE
                     KVDVAEIAAEYGGGGHVRASGCTVPGPLAEVKSNVISRLNRALAEAGYTWTE"
     gene            complement(77718..78095)
                     /gene="rbfA"
                     /locus_tag="PLU15_01000"
     CDS             complement(77718..78095)
                     /gene="rbfA"
                     /locus_tag="PLU15_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOO29531.1"
                     /translation="MSSYRPDRVKAAIRQEVSNILQRDLKDPRLGFATVTDVDISGDL
                     QHVKIYVSILGEEESRTETMKALESAKGYIRSEIGRRVPLRLTPEISFEYDESIERGA
                     RILKLIEEVQSGRNDEESGDMPK"
     gene            complement(78128..78394)
                     /locus_tag="PLU15_01005"
     CDS             complement(78128..78394)
                     /locus_tag="PLU15_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOO29532.1"
                     /translation="MRCVLLIEGSRSLKEKKRVIQGMMARIRHRFNVAVAEVDCQDIW
                     NKAQIGIVCVSNDSGHAHSILEAVARMIDLSPDVVLYSYEIEIL"
     gene            complement(78429..80999)
                     /gene="infB"
                     /locus_tag="PLU15_01010"
     CDS             complement(78429..80999)
                     /gene="infB"
                     /locus_tag="PLU15_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOO29533.1"
                     /translation="MGKVRVFELARDLNMQSKDLVDLLNELGVKAKNHMSSIDENAAR
                     YVMRKYGPDAPGAKADAQQKDRKSEESAQQAEPARKPEQSPPSAQGSARQEEKVQRQP
                     GGGRAYPQAASRPAGAGKPQGARQEGGAAPPYRDDRRDRGGNSGPVRDRSRLPGDASH
                     AGGRQNSERGLGASAQQQPRAPQGSPAKQQGKGRQRPSEGGRGRREDYVDDELGPRRA
                     ERKGDRRGRKKPPTSKPLEDARVLEHRVRTGDIEDIDEIAVKRGGRKREKAPKVRRIE
                     VGETISVRELAAKLEISASDCLRRLMKLGVMVRINDEIEGEVAQILASELGFEAELTS
                     EREERELVIAEIEDPPESLKPRPPVVTIMGHVDHGKTSLLDAIREANVTATEAGGITQ
                     HIGAYQVELDGRKITFLDTPGHEAFTAMRARGAHATDVAVLVVAADDGVMPQTVEAIN
                     HAKDAKVPIVVAVNKIDKPDANPERVATQLSERGLVPEKWGGDTVMVPVSALHKQGIE
                     DLLEMILLIADMSELKANPDRPAMGIVIEAKVDKGRGPVATVLIQKGTLRVGDYFLVG
                     QVHGRVRAMTNYKGEPVKEATPSMPVEVLGLGDVPLAGDVFQVVVDEREAKQVAARRA
                     ERRRIETIGEAPKKVSLEELFSQIQEGEVKELRVIIKADVQGSVEAVRQAIEQLSTDE
                     VRVDVIHGGVGAISESDIMLASASNAVVIGFNVRPDPVASMCAEDEKVDVRLYRVIYD
                     AIDDVKKALEGLLEPEYREVVYGQAEVRATFKVPKAGVVAGCYVTEGRVPRSASVRVV
                     RDGVVVHEGKIASLRRFKDDVREVAAGFECGIGIENWNDVREGDIIEAYGKEEVKREL
                     "
     gene            complement(81181..81447)
                     /locus_tag="PLU15_01015"
     CDS             complement(81181..81447)
                     /locus_tag="PLU15_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOO29534.1"
                     /translation="MAKARKVPQRTCVGCGAVRDKRDLVRVVRTPEGQVLLDPSGKRA
                     GRGAYLCPNRDCLAKAARGGKLSRALETDMPDEIIARLQEELSD"
     gene            complement(81502..82539)
                     /gene="nusA"
                     /locus_tag="PLU15_01020"
                     /pseudo
     CDS             complement(81502..82539)
                     /gene="nusA"
                     /locus_tag="PLU15_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
     gene            complement(82532..83011)
                     /gene="rimP"
                     /locus_tag="PLU15_01025"
     CDS             complement(82532..83011)
                     /gene="rimP"
                     /locus_tag="PLU15_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOO29535.1"
                     /translation="MDRRQIESRANAISQSAADSLGLEVVDTEFVKDSGTWYLRVYIE
                     KPGGVGIDDCADLSRLVGSELDREDFIPQSYILEVSSSGEKPIRRDEEYDRFEGRWAL
                     ITTYKEIDGRKRFEGVLRGNRGDSVLIEVDGVVVQVPRSSINTARLAVRLEEVFGDD"
     gene            complement(83216..86932)
                     /locus_tag="PLU15_01030"
     CDS             complement(83216..86932)
                     /locus_tag="PLU15_01030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOO29536.1"
                     /translation="MSTVFVLRPSSEKAQLKHIFKSAGMSVPLALYDSCSIDTICIDS
                     ESKHWTVRLAGAVFPGAECIDQICAAFRKVLGNAITVSVEPASGGPDDWTPSDEIIEK
                     YWPDVVERVCKNSPSVGVWLAQARCRADSVGVIVEVPGVVQVAKLKEKAGDRDIAQAF
                     SELCDHCPKVRIEVGEFEEPAVPVELDEPMAPLESVVSAKAERQGSSSRSPGRRRSRG
                     KPAEGAILGRMISDSPQSISELHEGQKKVVVAGQVFKFEERHTRNGFIITEFAITDKS
                     DSIMCKCFFRGDESRFHLESGQWVRVRGEVQYDHYAREIVLMASDIVPHQIEEKIDLA
                     SEKRVELHLHTKMSSMDSVCNVEDAVVRAASWGHPAIAITDHGVAQAFPDACNVARKH
                     GIKVIYGIEGYLVDDPSDENGHTYHVTLLAKNLEGLRDLYRLISLSHLEHFYRHPRIP
                     RSELEQARSNMLIGSACGAGELFRTILSGADDDEVAKVASFYDYLEIMPSGNNAWLVR
                     SGRLASIEEVEEISVRIYRLGKELGIPVVATGDVHFLDPQDEVYRRILMAGQGYEDAD
                     HQAPLYFRTTEEMLREFSFLGPDASYEVVVANTRQIADEVEAVRPVPDELATPEIEGA
                     DEEVRTRARARARELYGEPLPEIVNVRLERELDAICGNGYAVNYDIAAKLVHKSVSDG
                     YPVGSRGSVGSSFVASMCGITEVNPLPPHYLCSECKLSVFDHGHDVESGYDLPSANCP
                     RCGASMRNEGQNIPFETFLGFEGDKVPDIDLNFSSEYQSTVHKYAEEIFGADHVFRAG
                     TIATVAEKTAYGFVKAYCESRGIVKRSVEMDRLALGCTGVKRTTGQHPGGIMIVPGSR
                     DVHEFTPVQRPANDRDSDTITTHFDYKNIHECLLKLDLLGHDDPTMIKMLEDMTGVDA
                     STIPMDDPDTMKIFSGVESLGLDPETSGFVIGTVGIPEFGTKFVRGMLEQTRPTTFGE
                     LVRISGLSHGTDVWLNNAQELIESGQARLSEVIACRDDIMNRLISDGIAPRDAFRIME
                     RVRKGKMLADGDPELMREHGVPEWYIDSCNKIKYMFPKAHAVAYVMMAFRIAWFKVHW
                     PREFYAAYFSIRASDFDASIVGMSMEDIIATRNAIEEKNDATSRERNLATVLEAAQEG
                     MMRGIRFLPVHVCHSDAARFVIEEEGLRPPLITVPTLGDTAASSVIEARRERVFTSVH
                     DLWSRTKLTRTHIDTLEEIGATAGLPESDQLSLF"
     gene            complement(86961..87590)
                     /locus_tag="PLU15_01035"
     CDS             complement(86961..87590)
                     /locus_tag="PLU15_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO29537.1"
                     /translation="MRVAAIIPAYNEGPRIASVLDVVRASPLVDEIIVVDDGSDDDTA
                     EVARAHGANVIRLGENTGKGGAVARGLEATLGDVILFLDADLIGLTVDHVNALIEPVC
                     SGEADMTIGIFGNGRTATDLAQALTPWLSGQRAIKRSVLEGLELDISRFGVEAVLTRH
                     AKESGLKVVDVKLPRLTHVMKEEKMGTVKGMASRARMYWEVLKSIVDWD"
     gene            complement(87603..89342)
                     /locus_tag="PLU15_01040"
     CDS             complement(87603..89342)
                     /locus_tag="PLU15_01040"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO29538.1"
                     /translation="MRMSQMFAPTLREVPSEADIPSHQLMLRAGMVRKTVSGVYSYLH
                     LGCRVVRKIEKIVREEMDRQGAQEVLMPAIQSADIWRETGRWDDYGSEMFRLKDRNGR
                     EMCLGPTHEEIITVTVRDEIRSYRQLPKILYQIQTKYRDEIRPRFGVMRSREFIMKDA
                     YSFDRDNEGLDRSYEKMYEAYCRVFSRCGVDYRVVEADAGAIGGSQNHEFMVVSEVGE
                     DALVFCDACGYAANVERAESAKPAERVADTEKVRTGIRKISTPGMRTIEEVTGYLKVE
                     PRVLLKTLVYVADGTPVAAIVRGDRDLNEAKLKRVLGCRELELATPEVIERVSGAPVG
                     FTGPVGLRERIKIVADWEATVVEDAVVGANAVDAHLVGAEYGVDYTADIVADIRNVVA
                     GDACPRCGAELCGARGVEVGHLFKLGTKYSSALGAKYLDEGGVEHPIIMGCYGIGITR
                     TVASVIEEHHDDDGIIWPMSVAPYQAVVMPLNTSESAQVEAAERIYSELCKHGVEAVM
                     DDRDERPGVKFKDADLIGFPIRVIVGPKHLGDGEVEVSMRRDGTVELVKIENVAVRVK
                     EIVDRELAALMPE"
     gene            complement(89365..90444)
                     /gene="ispG"
                     /locus_tag="PLU15_01045"
                     /gene_synonym="gcpE"
     CDS             complement(89365..90444)
                     /gene="ispG"
                     /locus_tag="PLU15_01045"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOO29539.1"
                     /translation="MATREETRQVMAGSVPIGGGAPISVQSMTTADTRDADAAIKQIR
                     QLAAVGCEIVRVAVPDEEAADSLRSIRKASSVPLVADIHFNYKLALASIEAGVDKLRI
                     NPGNIGGADRVREVAHAAADRGIPIRIGVNAGSLPADLVSKYGRTPEAMVEAALRHVS
                     LLEREGFESIVLSLKASDVKRTTEAYRLIARRAPYPLHVGITEAGPPPGGLIKSAIGI
                     GMVLSEGLGDTIRVSLTGDPRDEVIAGREILASLGLRRFGPTIVSCPTCGRCRTDLAG
                     IVKEVKDGLSHLDAPLVVAIMGCEVNGPGEASEADVGLACGNGVGLIFSGGRVVRKVR
                     EAEMVGALVEAAESMAANRHEKSHG"
     gene            complement(90484..91524)
                     /gene="rseP"
                     /locus_tag="PLU15_01050"
     CDS             complement(90484..91524)
                     /gene="rseP"
                     /locus_tag="PLU15_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOO29540.1"
                     /translation="MYALAFLVVLGIVVLVHEYGHFAAARACGVKVYEFAIGFGPQLF
                     SWKRGETLYAVRALPLGGMCRLAGMDDSDLPEEQMEEDDPRSFLNRPIWQRILIVVAG
                     PLMNFVLAVVILSVLFSSYGVPMASVVEVFDGSPAEAAGFESGDKIVSIDGKPVASVA
                     GFINTVASSAGKKLIVEALRDGETIIIDVTPIRDAEENIGRVGMSLVEVYSKTGTGAA
                     IVGSLRYTWGIVDTTLASMGAALAGRVKPEVAGPVGIAGMSAQAARAGFPSLMMLVAL
                     ISTSLGLLNLMPIPFLDGGWVLFLIIEAVRGKPLSPQVEGFLRFIGLALLMLLLVYAT
                     VSDISRIMVQRM"
     gene            complement(91512..92666)
                     /gene="dxr"
                     /locus_tag="PLU15_01055"
     CDS             complement(91512..92666)
                     /gene="dxr"
                     /locus_tag="PLU15_01055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOO29541.1"
                     /translation="MTSICILGSTGSVGVQSLEVARSLGLKVSGLSAYSEVELLAEQA
                     RQFGPAMVGIGDVRAEARARALFSRMSTPPELFFGPEGVSQVAAAAEHDLVVNAIVGF
                     AGVWPTLAAIDAGHDVALANKETLVAAGSVIMDRARQEGVAIRPIDSEHSAIYQCIQG
                     QGREAVARIILTASGGPFIDRYDFADISVAEAISHPNWNMGPKISVDSATMINKALEV
                     VEARWLFDVDSEMIDIVVHRESVIHSMVEYCDGSIIAQLGTADMRTPIQYALTCPERV
                     EGLAAKLDLSSIGKLTFEKPDLSRFPGAGLGHEALRHKGVVPAVMSAANEEAVRLFLH
                     GDVRFVDIVDLVMEAMESSPRIEEPSLDDIVEADVWAREFVRGRCRSCTR"
     gene            complement(92670..93713)
                     /gene="ytvI"
                     /locus_tag="PLU15_01060"
     CDS             complement(92670..93713)
                     /gene="ytvI"
                     /locus_tag="PLU15_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOO29542.1"
                     /translation="MSNNAKAAISLGGLIVGTYLVIMYVLPLFRPFVIALFLAIMIEP
                     GVKLVQKNPKISRGVAVAIVLVVLGLVLGLLIALGVTEIAKEIDQLSRNMGGLSRSFS
                     EALDNLVGRTEDFFAYLPVPFVEALRENQDKLFQVVRTGVSGVASFLGGLPEFTLVFM
                     LSVISTFFISRDHPRFSENLLKALPRSWRKQARKVRGELLAGFVGYLRSRFVLMCMTG
                     IMTVIGFSIMGISYAWLLGLVCALLDLMPVVGPSLLILPWAGYHFVVGHFGFGLGLII
                     VLAVNSIVRQIAEGKVMGENLDLHPLVALVSIYVGIRLFGTKGFIAGPLAVIFIKAVY
                     QSVILPMFPFDEE"
     gene            complement(93748..94560)
                     /locus_tag="PLU15_01065"
     CDS             complement(93748..94560)
                     /locus_tag="PLU15_01065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOO29543.1"
                     /translation="MIRTRVLVGLIGAPIGIFVLWLGGVPLAIVGAVLAVVGGMEYAR
                     MCKTKAWTPSQPLISAGSALFVVDAVLTGGRFSGSLLSLVLALSLAGQVMKAKPTGSM
                     AVPALEVFGVVYCGWTLSRFCLIRGTFGDAGLGLSLLMMVLVWFNDMGAFFVGRAIGR
                     RKLAPSMSPNKTIEGSIGGFVWAVLASIAYKALGDVTGLWYTLSWPQAIIFALLVAAS
                     GQIGDLAESGMKRDAGVKDAGRMFPGHGGVLDRFDSFFFVSPIAYYYLRLML"
     gene            complement(94557..95288)
                     /gene="uppS"
                     /locus_tag="PLU15_01070"
     CDS             complement(94557..95288)
                     /gene="uppS"
                     /locus_tag="PLU15_01070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008885860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOO29544.1"
                     /translation="MLPKHIGIIMDGNGRWAQQQGKPRLYGHQVGANRAKDIVLAARD
                     RGIGYVTLFAFSTENWKRPISEVQGLFLLMEEFFRENADELAESGAKVNVIGDRSRLP
                     RSIRTVAANAERITANASRIIVNIALNYGGRWDVALALRRIAEDVAAGRLDASDIDED
                     LISSRLATAGQPDPDLIIRTGGESRISNFMLWQVAYAEIYVTQTLWPDFRDEDLDAAI
                     SFFLSRTRRYGGLKTAPHNGRGEGG"
     gene            complement(95418..95939)
                     /gene="frr"
                     /locus_tag="PLU15_01075"
     CDS             complement(95418..95939)
                     /gene="frr"
                     /locus_tag="PLU15_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOO29545.1"
                     /translation="MKEVVESTSRDFAGVRTGRANPALLDRIVVDYYDTPTPLTQIAS
                     VSAPEPRLLVVTPWDKSVLGKIEKAIQKSDIGLVPTNDGNVIRMAIPHLTEERRRELV
                     KLVRKMAEEKRISIRGIRRDAIEEFRVLEKDGIITEDELRRAQDEIQKLTDKYIADID
                     NMLADKEVEIMQV"
     gene            complement(95978..96697)
                     /gene="pyrH"
                     /locus_tag="PLU15_01080"
     CDS             complement(95978..96697)
                     /gene="pyrH"
                     /locus_tag="PLU15_01080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOO29546.1"
                     /translation="MERPKYSRVVLKLSGEALAGERGFGVDIDTVRWIAGQVSEAYAL
                     GTQLAIVVGGGNIWRGSEASRMGMDRSSADYAGMLATVINSVVLQEALEDSGIPTRVL
                     TAIEIREVAEPYIRRRAIRHLEKGRIVIFGAGTGNPFFSTDTAAALRAAEIEADVILM
                     AKRVDGVYDRDPLKNPEAKFYEKLSFIDVISRELGVMDSTAASLCMDNGIPIVVFNLL
                     TEGNIKRALMGEPVGTWIGGD"
     gene            complement(96823..97422)
                     /gene="tsf"
                     /locus_tag="PLU15_01085"
     CDS             complement(96823..97422)
                     /gene="tsf"
                     /locus_tag="PLU15_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOO29547.1"
                     /translation="MEITASMVKELRERTGAGMMDCKRALVETNGDFDNAVTLLREKG
                     LAAASKRAGRETAEGVVESYIHMGGRIGVLIEVNCETDFVAKTDQFRTLARDLAMQVA
                     ASRPLYVSRDEVPEDVIERERQIYRTQALNEGKPEKVADRIVEGKLEKFYQEVCLLDQ
                     PFIREPEKSVSDLVRETIGTLGENITVRRFARFERGEAL"
     gene            complement(97443..98243)
                     /gene="rpsB"
                     /locus_tag="PLU15_01090"
     CDS             complement(97443..98243)
                     /gene="rpsB"
                     /locus_tag="PLU15_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO29548.1"
                     /translation="MAVVTMKQLLEAGVHFGHQTRRWNPKMKPYIFTSRNGIYIIDLQ
                     QTVRKLDEAYDFVRETVQDGGTVLFVGTKKQARDTIAEEAQRCGMYWVNERWLGGMLT
                     NFATMRKRVEWMKKLEQMEEDGSFDALPKKEVQKLIAERTKLQKRLIGIRDMKQLPSA
                     VFVVDPRKERIAVAEARRLDIPIIAIVDTNCDPDEVDYVIPGNDDAIRAVRLLTSKIA
                     DAVIEGREGLDQALAEEEQDEGFSADGNNGEFQASAKSDDADEFEDAE"
     gene            complement(98533..99327)
                     /gene="codY"
                     /locus_tag="PLU15_01095"
     CDS             complement(98533..99327)
                     /gene="codY"
                     /locus_tag="PLU15_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOO29549.1"
                     /translation="MNSLLERTRKLGRLIQSSPGHAGRFEEIAHALAEAVSGQAFVVA
                     KNGKILGHSLPHLTDDDFEVLDSEILGNGYVPQKVNEEFLAIFETAANRRPSNGMRAF
                     CNEMLGGSSESCRLTVAPIYGGGERVGTLLLLKSDGELGENDIMLAEYGATFIGMEIY
                     RSRSEKAEEEARKRAAVQMALETLSYSEQEAVEHIFDELAGDEGLLVASKIADRVGIT
                     RSVIVNALRKFESAGVIESRSLGMKGTYIRVLNDKLFDELDRLRAR"
     gene            complement(99447..100841)
                     /gene="hslU"
                     /locus_tag="PLU15_01100"
     CDS             complement(99447..100841)
                     /gene="hslU"
                     /locus_tag="PLU15_01100"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOO29550.1"
                     /translation="MQDLTPRAIVRELDKYIVGQASAKKAVAIALRNRQRRLALAPEM
                     QDDIIPKNILMIGPTGVGKTEIARRLARLVNAPFVKVEATKFTEVGYVGRDVDSIVRE
                     LVETSVRMVKAEKMSGVQEQAKILGEERLLDALAPLPTRDRSQANPFAALWGMPVGQP
                     QDDRMFESKLASARARREETRERLRRGELEDVIIEIEIDDVQGGMIEIFSGAGVEEMG
                     INLQDLMGEMLPPRRRRRKLPVSEARKILITEEAAKLIDMDEVAEEAIARAEQSGIVF
                     IDEIDKIAVKGQGAGPDVSREGVQRDILPIVEGSTVQTKYGPVKTGHMLFIAAGAFHM
                     SKPSDLIPELQGRFPIRVELSSLTKDDFVRILTEPKNALTKQYVALLATDGVDLVFTD
                     DGVEEIARIADEVNSSSENIGARRLHTILEKVLEEVSFNAPEHSERVVVDADYVRSRL
                     EQLVTESDLSDYIL"
     gene            complement(100857..101387)
                     /gene="hslV"
                     /locus_tag="PLU15_01105"
     CDS             complement(100857..101387)
                     /gene="hslV"
                     /locus_tag="PLU15_01105"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOO29551.1"
                     /translation="MRMTGTTVVAVIRDGKCAMGSDGQVTLGDTVVKHGARKIRKLSD
                     WNVLAGFAGSASDSMALLERFEGKLSEYKGNLVRAASDLGREWRTDRVLRRLEAMMIV
                     TDGNRILLLSGNGEVLEPDDAITAVGSGGPYALAAGRALLRNTDLEAVEIVRKSLEIA
                     SEICIYTNGKINIEEL"
     gene            complement(101442..102344)
                     /gene="xerC"
                     /locus_tag="PLU15_01110"
     CDS             complement(101442..102344)
                     /gene="xerC"
                     /locus_tag="PLU15_01110"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOO29552.1"
                     /translation="MDHLDDFARYLEAERNLSPRTVDAYIRDVGQFISFCEKSGTWQG
                     IAGTGVDAYQLSMLRKYLATLQSRGLAHSSISRKMSAIRSYLKFLAREGVIESNPTVA
                     MSQVRRPRRLPRTLSQQEVERLISVCGDGSPLDIRDRAILELLYSSGIRLAELVGLDV
                     KDYNASSGTVRVFGKGAKERIAPVGQAAVRALDDYIANARPHLASNSEETALFLNRGG
                     GRLSGRSVERMMKKRLMEAGLPQTATPHSMRHSFATHLTDSGADLRAVQELLGHADIS
                     TTQIYTSVSKERLYRVYNKTHPRA"
     gene            complement(102465..103781)
                     /gene="trmFO"
                     /locus_tag="PLU15_01115"
     CDS             complement(102465..103781)
                     /gene="trmFO"
                     /locus_tag="PLU15_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOO29553.1"
                     /translation="MQNEVIVIGAGLAGSEAAWQASQMGADVKLYEMRPIVSTPAHST
                     GNFAELVCSNSLGSDREGTAPGLLKEELRALGSIIMRCADRTRVPAGQALAVDRGLFS
                     ALVTKELEAAPNIEVIREEVVRVPREGVVVIATGPLTSPAFSEEIASLLGTDHLYFFD
                     AMAPIISGHSVNWSRAYWASRYGRGDSDYANCAMNREEYELFWGNLIEAETAEMHEFE
                     SLQLFEGCMPIEEIARRGRDTLRYGPLKPVGLVDPRTGRRPYAVVQLRRENVPGSLFN
                     MVGFQTRLKQGEQSRVFRMIPGLEEAEFVRYGMMHRNTFVNGPEHLSATLQLKESERI
                     FMAGQITGVEGYMESVATGLVCGMNAGRLAMGKDPLTFPSKTAIGSLCSYVSTPCKGR
                     YQPMNVNFGLLPEPPRALKKNERRMWKVALARQSLHDFAGSLRGGC"
     gene            complement(103771..105897)
                     /gene="topA"
                     /locus_tag="PLU15_01120"
     CDS             complement(103771..105897)
                     /gene="topA"
                     /locus_tag="PLU15_01120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOO29554.1"
                     /translation="MGKTLVIVESPAKVKTLSKFLGRQYALKASVGHVRDLPKSQLGV
                     DVDNDYAPKYITIRGKGDVIRDLKESAKKADRILLATDPDREGEAISWHLAYILGVDP
                     ESDCRIEFNEITENAVKNAIKAPRPIDMGRVEAQQARRVLDRLVGYKLSPFLWKKVRP
                     GLSAGRVQSSALKIICDREREIEAFESEEYWTLGAELSPKPRGRSFEARFIPEEDKRL
                     SKEEVDAVIADLEGADYVVESINVRDQARKPAPPFITSTLQQEAFRKLGFSGKRTMSN
                     AQALYEGVEVAGEGHVALITYMRTDSTRVAQEAQAAARDYVRKTWGAKYVPKSPRVYA
                     SRKGAQDAHEAIRPTSVERTPESLKGSLGRDQLRLYTLIWSRFMASQMSDAVNEITSV
                     DISAGEHRFRATGQKVKFDGFTVIYTEGRDDESSKNDDKRQLPALEVGQRLDLVQLLP
                     EQHFTQPPPRYTEATLIKELEKHGIGRPSTYAPIIDTLRKRDYATLDKKRFVPTEVGF
                     AVCDLLSEYFPSVVDLEFTAKMEEKLDSIEENEADWVSVTDEFYKPFAQKLAEAEELA
                     EKVVIEDEPIGEACPECGAELVIKSGRYGKFIACPMYPKCTYRRSIVKKLDVKCPKCG
                     GDVVERRSKKGRTFYGCSNYPNCDFVAWNRPSKVSCPMCGSFTTVRQRKDEMIYKCAD
                     AECGHTWKEAADSGSGAQQGGTGVAE"
     gene            complement(106011..107159)
                     /locus_tag="PLU15_01125"
     CDS             complement(106011..107159)
                     /locus_tag="PLU15_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOO29555.1"
                     /translation="MDDKEPKLRWENVAEPEGKELAAWVFWNSVPGFGSRSFLRMLED
                     YGSAVEGLHAAKHGGKPPAYARRAQWNWAGRVHDPLCMGMTEIAKASSISARVIPCPS
                     RLYPAPLRNIPDPPIVLYVLGKGELTEVPWAAMVGSRQATAYGISIARRYGRMLASAG
                     IGVVSGLARGIDASAHRGAIEGGGPTVGVLGAGMGDDMTGRLKKLIREVAACGCVISP
                     FPMGWAASKDTFPARNRIISGMSEVCIVVEAAEKSGAKITADFANEHGKDVFAVPGDI
                     NRPTSVGANQLLSTHAEPLLDIGDVIDRIASRGLLDRTHGPTARISGGVLAPVDRAVL
                     ESLTVNTSFERIQTDLGLPAQELLVSLARLEFAGLVDREPGMRYSKRC"
     gene            complement(107152..108687)
                     /locus_tag="PLU15_01130"
     CDS             complement(107152..108687)
                     /locus_tag="PLU15_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOO29556.1"
                     /translation="MLSAARGCALQGITGMSVTVECDLARGLPSFDIVGLPDPTVRES
                     RERVRAAIRNSGYEFPLSRITVNLAPADVRKVGVGLDLPIAVSILAASGQSPHKGLEG
                     ITFIGELSLEGVIRPVRGVLPACAGAREAGSTVVVVPVENAGEAMCIPEVSVVAVRTL
                     QEVVRFLETGIVPNRDISTKHAHLGANLGPDFSQVIGQAATVRALEVAVAGGHNIIMV
                     GPPGAGKTLMASCVPSVLPAMDREEAIEVTSIQSIMGLVDGRGLAVSRPFRNPHHSIT
                     PAGMLGGGNPPMPGEVTLAHRGVLYLDEYCEYRQAVLQCLRSPMEDGFVVISRSHVQV
                     AFPSRFMLVASANPCPCGYLGDSERPCVCSGAQVQRYRRKLSGPVIDRIDIQIEAPRV
                     SRADLQGHSAGRSSDKIRANVSEARERQRRRFGNPIVTNSCMDPRNIEHAAMLSPIAG
                     ALLYEAVDKMALSARACHKLIKVARTIADLDASARIKDDHVLEALQYRALDSAEIARC
                     SHG"
     gene            complement(108709..109074)
                     /locus_tag="PLU15_01135"
     CDS             complement(108709..109074)
                     /locus_tag="PLU15_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOO29557.1"
                     /translation="MGSQDVGRAGEEFARSYVERLGYEILGCNMRFGRKEVDILAVDG
                     DTLVVIEVKTRLTWDYGMPEEQISAAKLRSLKQIASVVSAACGGATVRVDVISIRAFG
                     FGACGLRDANLSHIKNVIS"
     gene            complement(109176..109829)
                     /locus_tag="PLU15_01140"
     CDS             complement(109176..109829)
                     /locus_tag="PLU15_01140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOO29558.1"
                     /translation="MTGIMAEEVVRSDIAMFDDSLRNAGFHVIAGVDEAGRGPLAGPV
                     VAAVVVLREGFYLPGVDDSKRLSAKKRQAVLEEIMESALEVGVGVAWQDEIDEVNIRV
                     ASLRAMSRAVDQLSVVPNLVLVDGKDLPDLRLPAVALVRGDARSQSVAAASILAKVVR
                     DALMCELAIVYPEYGFERHKGYPTPEHLRNLAAFGPCALHRKTFRGVSQSADESVDT"
     gene            complement(109826..110668)
                     /gene="ylqF"
                     /locus_tag="PLU15_01145"
     CDS             complement(109826..110668)
                     /gene="ylqF"
                     /locus_tag="PLU15_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOO29559.1"
                     /translation="MGFSWYPGHMAKAVSQIKQSMKLIDVVIEILDARAPRSTENREF
                     AEAVGNKPRIVVLNKADLADPVATRAWVDCYTEAGICAVPTNCRTGEGVAEVIAAASA
                     AAKAGAGPDVQRGPSRVRFRTAVLGIPNVGKSSFINRAAKRARVKTGDRPGVTRAKQW
                     IVIDRNLEMLDTPGIMPPRVDDRGVWFALAALGCLDESIYDVEALATGLIARLDSVGL
                     AAYRSRYGVCEGCVDPHEALKHVARSRGCMMAGGELDMARAADILVRDFRSGKLGRAT
                     LEMP"
     gene            complement(110736..111299)
                     /gene="lepB"
                     /locus_tag="PLU15_01150"
     CDS             complement(110736..111299)
                     /gene="lepB"
                     /locus_tag="PLU15_01150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO29560.1"
                     /translation="MEYVNACIVAVVLAAFIMTFVARSFVVQGSSMEPTLYNGERLLV
                     NRFIYRFREPQRGDIIVFRYPQDPRQMFIKRVIGVPGDELMISGGMVYVNGTGLNEPY
                     IAEPSTREYGPALVPTKRVFVLGDNRNNSQDSTDDSVGMVPFGSIQGKAFVVYWPVDR
                     IRTLKNPTCLEAIGTPKSGMAAAPGMP"
     gene            complement(111543..111890)
                     /gene="rplS"
                     /locus_tag="PLU15_01155"
     CDS             complement(111543..111890)
                     /gene="rplS"
                     /locus_tag="PLU15_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOO29561.1"
                     /translation="MNIIDTIEREQMRSDLPDFGPGDTVRVHVRVVEGARERIQVFEG
                     VVIGRSGSGLSETFTVRKMSSGIGVERIFPVHSPKIDKVEVVRQGDVRKAKLYYIRGR
                     VGKRARIREKRQH"
     gene            complement(111987..112730)
                     /gene="trmD"
                     /locus_tag="PLU15_01160"
     CDS             complement(111987..112730)
                     /gene="trmD"
                     /locus_tag="PLU15_01160"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOO29562.1"
                     /translation="MEIDILTIFPGMFYGPLSESILGRAQEKGILTVRIHDIRDFSTS
                     RHRVVDDYPFGGGPGMVMKAEPIAAAVEHALAARVQAKESRSRVIMTTPAGSTFSQDM
                     AKGFASCDHLVIICGHYEGVDERVMSLVTDRVSIGDYVLTGGELPAMVIVDATARLLP
                     GVLGDEESASADSFWDGILDYPHYTRPRVWRGHEVPDVLLSGDHERIRKWRRKQALKV
                     TLEQRPDLLSQVELTDEDVELLKQVMEDG"
     gene            complement(112801..113325)
                     /gene="rimM"
                     /locus_tag="PLU15_01165"
     CDS             complement(112801..113325)
                     /gene="rimM"
                     /locus_tag="PLU15_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOO29563.1"
                     /translation="MTRSGDQLVLIGVIMGTHGLWGELRVRPENGDPRRFCGIDRVFL
                     GRENCADFQERKVISERAHGEASLVLLEGVEDVETGKLLKGFGLYLPVEELPELPEDE
                     FYAFDLVGLGVYAKTGERLGIVKEVIELPANDVFVVKPDSGREFLVPAVGDIVCEIDV
                     AERRMIIDDRPGLR"
     gene            complement(113322..113582)
                     /locus_tag="PLU15_01170"
     CDS             complement(113322..113582)
                     /locus_tag="PLU15_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOO29564.1"
                     /translation="MAGNKKNEGRLCQLVTFLARNLVDDPEEVRVREIESGDSVIFEV
                     SVAPDDMGKVIGRHGRVARAIRSVTKAAAIRDGKRVMVEIVD"
     gene            complement(113595..113909)
                     /gene="rpsP"
                     /locus_tag="PLU15_01175"
     CDS             complement(113595..113909)
                     /gene="rpsP"
                     /locus_tag="PLU15_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOO29565.1"
                     /translation="MAVRIRLTRTGAKKQPHYRLVVADSRSPRDGRFIEILGHYNPRM
                     DPPELVVDVDRAAEWIRRGAQPSETARALLVKAGLDMSGASGKGTQSAEADVAPVDAA
                     EN"
     gene            complement(113973..115310)
                     /gene="ffh"
                     /locus_tag="PLU15_01180"
     CDS             complement(113973..115310)
                     /gene="ffh"
                     /locus_tag="PLU15_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOO29566.1"
                     /translation="MFENLSARLQDVFGNLRNRGRLTEKDVDAALREVRMALLEADVN
                     FKVARDFTARVRERAVGEEVMKSLTPAQQVIKIVMSELTELMGGTAAKISFAPRPPTV
                     IMLVGLQGSGKTTSCAKLANLLRKQGHKPLLVAGDVYRPAAIKQLQVLGGQLGIEVFS
                     MGDKTDPVDIAAAAVERGKSMGYDVVILDTAGRLHVDDEMMEELGRIKSRVNPTEILL
                     VVDAMTGQDAVNVASMFNERLGIDGIIMTKLDGDARGGAALSVRAVTGKPIKFVTTGE
                     KLDAVEQFHPERMASRILGMGDVLTIIEKAQDAFDAEQAMKMEEKLRKQEFTLEDFLS
                     QLQDVRKMGPIDQLLGMIPGLGKSKALKGVAIDEKDLARVEAIVRSMTPAERHNPGII
                     DGSRKRRIAAGSGVRVQDVNALLKQFEQTRKMLKQFGDAAKRGGPPARMPFMP"
     gene            complement(115328..115795)
                     /locus_tag="PLU15_01185"
     CDS             complement(115328..115795)
                     /locus_tag="PLU15_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HOO29567.1"
                     /translation="MLDKMLRMGALLDVHGPLLTEHQREMCVLYYLNDYSLAEISELY
                     GVSRQAVHDTLARAERAMEGHEETFGLLRMAEERRKRVGELDSQLSDADFQLEMAMAS
                     LDEAERSAQRPSGPDLAGVRRNIEQTMEIIASARRLVASIDSGDGGSPGMEDD"
     gene            complement(116183..117139)
                     /gene="ftsY"
                     /locus_tag="PLU15_01190"
     CDS             complement(116183..117139)
                     /gene="ftsY"
                     /locus_tag="PLU15_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOO29568.1"
                     /translation="MSPSFFSNLWSGLAKTRDAIIDPIEALFSKKKVIDEETLEELEA
                     VLIQADLGVAATTAIINKMRESGSRGEQVDADGAVALIEQELISILGERTAPLARADE
                     PPTVVMVVGVNGTGKTTSIGKIASMLAHEGNRVTLAAADTFRAAAIEQLEVWGNRTGC
                     NVVRHQEGSDPAAVAFDAYQAARARGSDYLLVDTAGRLHTKSNLMEELKKIKRVISRE
                     CDEAPHEVLLVVDATTGQNAIAQARVFNEAVGVTGVVLTKLDGTARGGIVVAISQELN
                     IPVKYVGVGEGAEDLRPFSPERFVQGLLRADAGDDGRKGAKR"
     gene            complement(117158..120712)
                     /gene="smc"
                     /locus_tag="PLU15_01195"
     CDS             complement(117158..120712)
                     /gene="smc"
                     /locus_tag="PLU15_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOO29569.1"
                     /translation="MYLKKLSLFGFKSFASRVDLDFSPGLTGVVGPNGVGKSNIADAL
                     RWALGEQSPRILRGSRMQDVIFAGTESRKPLGYAEVTLVFDNSDDFLNLGFTEVTVTR
                     RLFRSGESEYVLNGVPCRLRDIIDLFSGTGLGKDSYSVVEQSKIDIILSDRSQDRRAI
                     FDEASGIMKYRARRREAERKLVEVRADQLRVGDLVAELGRQLPVLRNQAEEAERWREL
                     TERLTRLEIDLLCHDLKRLSLRRLDSASRIDDLERREHECAAESAQCEAVLESAKTRA
                     MQADLAVETLHVAAGQAAAASERESGRLKLAQERKRTLLERSQEIAAACEAEEGKHAR
                     LENEFALLQGQVDAAGLEGRDVMEQADASRRRLAEASKARADAEARVEEAKSDLFDAL
                     ALESDLRSRIGAIESSKRSNEARLARLIQQAQEKAVAQDSARRETDDLFSELERLRGD
                     LEKAEREAADAKVLYERAMEAAESVSERAMEAEAKVSSAAAAHSSIDALQRQYEGYGR
                     AVRALLAGDNGRAMGLAGTVSDLIRVPKEYEIAIEAALGPSVQNIVASTSEGAERAVE
                     FLKKNRAGRATFLPLDILKPSPISSAQLPVAEPGIVGLAADLVESAPIYRKVVEYLLG
                     RVVVVRDLGCGIRLAKSGLRCRMVTLDGDIISAGGAITGGEAPDRRGGLLARSRRLEE
                     LAAELESARTESASIHRLKDEAVAEAGALRLVWEENERNKATLESVLSNVVQKIRLAE
                     AELSRFADELETVELEAEIVRREIDALTEEAKSLAQQLSGSEARRKKLEESLEVLAES
                     AAGLKEDESREAERYSELAQEAATYRERLLGLKAALLKAEEQLMSHNEELARLRNEEN
                     RVCQEATDAAAQIEAIGEAAAALAQEYSKAQRLLDEGREERAKTAADVNEGELAARVA
                     RKRHQEVAEKLNGARIEGARIAAEYEACVERLASAYSISEEEGLARDVAIQSRAGVQA
                     EIAQLRAEITEIGPVNHTAIEESRKLAERHGFLETQLKDLEAAQASLDEVVRECELTC
                     EKRFLETFEAVRSEFADIFTDIFGGGTADLVLDDPDNPLECGIDIVCQPPGKKLSSLS
                     LMSRGERSLVAIALLFAIMRVNPSPVCLLDEIDSALDEANLIRFAQLLKTTSEDVQVI
                     IVTHRKRTMECADALFGVTMEESGVSKVFSIRTDQQVS"
     gene            complement(120802..121062)
                     /locus_tag="PLU15_01200"
     CDS             complement(120802..121062)
                     /locus_tag="PLU15_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOO29570.1"
                     /translation="MEVLKVSGQSNPKAVAGALAAIVRDQGSVQVNAVGAAAVNQAVK
                     AITIARGFVAPNGIDLVTVPAFSEIEIDGEERTAIKFIVMPR"
     gene            complement(121155..121889)
                     /gene="rnc"
                     /locus_tag="PLU15_01205"
     CDS             complement(121155..121889)
                     /gene="rnc"
                     /locus_tag="PLU15_01205"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOO29571.1"
                     /translation="MCATIEIDKVSAVLGLEFEDKSLLVRALTHTSYAAEHGQDRALS
                     NERLEFLGDAILKAVMAEQLMTLMEDADEGRLSKVSAQAVSGQALARAAVDLGVSDFI
                     MLGHGEESSGGRCRQRNLAGAMEAIIGAVYLDSGFDEARSFIIRALASEIHDAITGPE
                     NDHKTAFQEAVQARSLGQASYRTVKSEGPPHKPWFTVEALIADRVVGTGEGPSKRAAE
                     QEAAKDGLRMLENGESAWANCASDDE"
     gene            complement(121864..122121)
                     /gene="acpP"
                     /locus_tag="PLU15_01210"
     CDS             complement(121864..122121)
                     /gene="acpP"
                     /locus_tag="PLU15_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO29572.1"
                     /translation="MTEAEILAKVREIVSDKLGVAEEEICRESDFIDDLGADSLYLNE
                     IVYEIEEEFDLRVPDEDFDSMRTVGQAVDYIYNRVCDDRDR"
     gene            complement(122214..123221)
                     /gene="plsX"
                     /locus_tag="PLU15_01215"
     CDS             complement(122214..123221)
                     /gene="plsX"
                     /locus_tag="PLU15_01215"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOO29573.1"
                     /translation="MRIAVDVMGGDMGPAEMIPGALMARDQYGAQVIFVGDEETIKAH
                     LKENGRDESDIEIVHAADSISMDEDPLAAVRHKRDSSMVVAAKLVREKKAQAAVTSGS
                     TGALVAAGPLVVGRLKGASRPALATPIPTVGGHPCVILDIGANPECQAKNLLEFAIMG
                     SAYASALLGIDNPSIGLLSNGTEASKGTSVIVQANQMLSGSGLNFVGNIEARSIFYGE
                     ADVVVMDGFAGNVVLKLSEGLGSAIFDMVKEEMSKGCMVKAGALLVKPALKSVKRRMD
                     YSEYGGAPLLGLSGLLVKAHGSSNRKAFANAIRVTSELLDRGLVEKMSQSLSAMVDGE
                     A"
     gene            complement(123321..123503)
                     /gene="rpmF"
                     /locus_tag="PLU15_01220"
     CDS             complement(123321..123503)
                     /gene="rpmF"
                     /locus_tag="PLU15_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006697063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOO29574.1"
                     /translation="MPAVPKQKSGKSRTRKRRANWKAEAPTLSVCPQCKKPRLPHRAC
                     PECGYYGGRTVIDVSE"
     gene            complement(123723..124232)
                     /locus_tag="PLU15_01225"
     CDS             complement(123723..124232)
                     /locus_tag="PLU15_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOO29575.1"
                     /translation="MQIDVEGLREEKGSNMSIDMAEDFPSIDLIRSELVFVEPVRFVG
                     QATNTGRRIVVTGIAYGRARATCDRCLSEFELPLESSILETYYRSDEAPDSPDEDERE
                     YGEDNIVDIAPAIEQAFILLLPIRIVCDEECKGLCPQCGHNLNWGDCSCPPDESWGLF
                     QRAKIIHKE"
     gene            complement(124255..125466)
                     /locus_tag="PLU15_01230"
     CDS             complement(124255..125466)
                     /locus_tag="PLU15_01230"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOO29576.1"
                     /translation="MKILVINCGSSSLKYQLFDMDEEKCLARGGVERVGSPDAFMTHT
                     RIGHDKVKRDVDAPDHERALKYVFDALVDPETGVIGSLSDISAIGHRVVHGGERFASS
                     VMIDEEVMKALHECQDLAPLHNPPNIMGIEACRRLMPDLPMVGVFDTAFHQTMPKEAY
                     MYGLPYDMYVNHGVRRYGFHGTSHRYVSQRAAILMKRPIEELSLVTCHLGNGSSIAAV
                     KRGKSIDTSMGFTPLEGLVMGTRSGDLDPAVVSFLCEQLDKSASEIVLGYLNKNSGVL
                     GLSGGLSNDFRDLEKAAAEGHQLARLALDVFAYRVVKYIGAYAAAMGSIDGIVFTAGV
                     GENSSDMRRRICDRLQLFGIELDEKKNSARGVEQIISTPESRVTVMAIPTNEEIMIAR
                     DTAEIVSALGA"
     gene            complement(125569..126453)
                     /locus_tag="PLU15_01235"
     CDS             complement(125569..126453)
                     /locus_tag="PLU15_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOO29577.1"
                     /translation="MWHRFRLILLPLLAAFCVVLLLWACPGANASAVTLRKGDRGENV
                     YELQEQLALAGYLDGEADGVFGNATYAAVIRFQNDVGLTPDGIVGPNTWSSLAQKAQS
                     ASYSKYVVAKGDTMYAIAKKFGVTVEEIAAASGVLRPELIKPGQELKIPICASAASRG
                     TSGRANAELLHWDVAKTIFTARATIIDCATGLSFQVQRRGGHNHADAEPLTAADTAIL
                     KQIYGGKWSWSRRAVIVLVGDRRIAASINGMPHGGCLIKDNNFDGHFCVHFLGSRTHA
                     SALLDPDHQRMVNVAAGL"
     gene            126586..127818
                     /gene="ylbJ"
                     /locus_tag="PLU15_01240"
     CDS             126586..127818
                     /gene="ylbJ"
                     /locus_tag="PLU15_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HOO29578.1"
                     /translation="MKPSLEPNRRNAYLVAALASFITIAIIALPEQAFQASLDGLRLW
                     WNIVFPALLPFFIASDTLMGLGVVHFIGALLEPLMKPLFGVPGVGAFALAMGLASGYP
                     IGARITARLRREGLCSRVEAERLVSFTNTADPLFMVGAVAVGMFQLPGLGPILAASHY
                     VSAILLGFLLKSYGESDKRRKTEEYRREESLLSRAAHELYTARSQDNRSFGQIFGDAV
                     KDSVGTLLLVGGCIMIFSVVIRVLTVFHITDLLSAPLIAVLTPLGVPQDLVTSLVGGF
                     FEITIGTEMASRATASIAQKAIAASAIIAWSGLSVFAQVASMLFGTDIRLTPYFFARI
                     VHAVLAAIVTAIMLKIVPWAAQQTIAIPGLSSSPGFLATVGYSCTMLAKTAAIGASIM
                     AALALLSKPRAFGFRVKK"
     gene            complement(127828..128277)
                     /locus_tag="PLU15_01245"
     CDS             complement(127828..128277)
                     /locus_tag="PLU15_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29579.1"
                     /translation="MIKQSVVECLNRMETIIESSGKLPFSGKRAVDGVELLELIDRIR
                     IALPEEMRQTEALIAERSRLAAEDGVGIASAQASGADGEVFASAREEAEVLLMRAERE
                     AMEIRLGADDYAEATLASLQETLDRTSFVVRKGIEELRRRKDNPEAR"
     gene            complement(128282..128770)
                     /gene="coaD"
                     /locus_tag="PLU15_01250"
     CDS             complement(128282..128770)
                     /gene="coaD"
                     /locus_tag="PLU15_01250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOO29580.1"
                     /translation="MLTLAVCPGSFDPVTMGHLDIITRAASIFDNVIVAILENQAKDH
                     MFSLEERLEMLRVACAHLPNVQVDHFEGLLVDYVRACGAHVVIRGLRAVSDFEFELTM
                     ALMNRKLDPSIETMYIMTSMEYSFLSSSIVKEVAGSGGDIHGLVPEPLVERVSARCRR
                     KG"
     gene            complement(128771..129340)
                     /gene="rsmD"
                     /locus_tag="PLU15_01255"
     CDS             complement(128771..129340)
                     /gene="rsmD"
                     /locus_tag="PLU15_01255"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOO29581.1"
                     /translation="MRVIGGIAGGRRLATIRGRNIRPTSDRVREAIFNIIADRVEDCV
                     VLDLFAGVGSLGIEAISRGARHCVFVDKDHAACGVIKRNLDDLGFEEASTVVEADAFR
                     AIGAGRGPVSEKAPYDLVFVDPPYAMEASEKLLSLIENARILAGDGLVLVEHSSRDIA
                     PYGSSRMTMVRQERYGDTEVSFYMFSSEG"
     gene            complement(129350..129583)
                     /locus_tag="PLU15_01260"
     CDS             complement(129350..129583)
                     /locus_tag="PLU15_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29582.1"
                     /translation="MSRLAAMTDNSCDASSNTMLDDRGCESCIDTVESESFELLKAPR
                     SIRRLVEAWDSRCWSLSRTYWGRELQLRLGPIA"
     gene            complement(129598..131295)
                     /locus_tag="PLU15_01265"
     CDS             complement(129598..131295)
                     /locus_tag="PLU15_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOO29583.1"
                     /translation="MSGTKQSPASQMKTIDGAALVAAFESGVAWLGVNKSLVDSLNVF
                     PVPDGDTGTNMYLTVSAALKEAEKAGSASVGDVADAIALGSLMGARGNSGVILSQLLR
                     GFAKRLHGLASANPTDLAAAFQEASSVAYKAVMKPVEGTILTVARTGARGGVQAAKNG
                     ADILGVIDEALRQGEAALEKTPEMLPALKEAGVVDAGGKGLVIFVQGFMKGLVGEPVE
                     PAALVHAEAETALKVAREPMAVKQEIKYAYCTEFIIRGNGLDPEKIKSRLLAEIDGDS
                     MLVVGNENTVKVHMHSNVPGRILEIGIGYGSLHEIDIDNMEDQHEEFEEMSGAENGVF
                     PHSSEKEPYELKDVGIVAVAAGDGVEKILQSLGADVVITGGQTMNPSIQDIVDGIREA
                     HAKDVIILPNNGNVVLTARQAAEVPDIEARIHVVPSKTIPQGLAALLAMTPYTSVEDN
                     VAKMNEAIGKVVSAEITYAVRDSTFNGIEIRENDIIGIVDGDIQVVGEDVDEVVRKVF
                     TEMYSEEHEIATIIYGFSVTEEEAESLADSLAEEYPDIEFEVQYGGQPLYYYLISIE"
     gene            complement(131295..131672)
                     /locus_tag="PLU15_01270"
     CDS             complement(131295..131672)
                     /locus_tag="PLU15_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOO29584.1"
                     /translation="MGKEISTELGQITIADEVIAAIAGMAATECYGLVGMASRTFTDG
                     IAELLGMDNIARGVEVDVQGDNATIDLYIIVEYGTRITEVANNVMDKVKYILETTTGL
                     NVPEVNVTVHGVRVGAGGKERQK"
     gene            131855..132046
                     /gene="rpmB"
                     /locus_tag="PLU15_01275"
     CDS             131855..132046
                     /gene="rpmB"
                     /locus_tag="PLU15_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOO29585.1"
                     /translation="MARVCDICGKGPVSGNSRSHSEIKTKRRWYPNLHQVRAEIDGST
                     RRIKVCTRCLRSGKVTRAI"
     gene            complement(132166..133023)
                     /locus_tag="PLU15_01280"
     CDS             complement(132166..133023)
                     /locus_tag="PLU15_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010498286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO29586.1"
                     /translation="MLEGRVDLHCHTTKSDGTFTPTELVSEAARRGLAAVAITDHDCV
                     DGIGEALEAVEENCVDVVPGVEISTDVSDGEIHILGFFIDYEDEDLLKFLEAQRESRV
                     RRVERMLRKLAALGIDLSMEDVLEHAGDGAVGRPHIAAAMVKAGYVLSWDEAFAHYIG
                     LHAPAYESRSKLTPYEAIEAVLAAKGVPVLAHPGLSGKDDMIPSLVEHGLEGIEAIYP
                     QHTSGQQEHYQRIAREYGLLVTAGSDCHGPYSQSGVVLGSATTDYEVVLRLYERARRT
                     QAMICEKRT"
     gene            complement(133004..136126)
                     /gene="ileS"
                     /locus_tag="PLU15_01285"
     CDS             complement(133004..136126)
                     /gene="ileS"
                     /locus_tag="PLU15_01285"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOO29587.1"
                     /translation="MFKKVDSAMDFPRMEEEILEVWREEKIFEKSVDRREKGPRFVFY
                     EGPPTANGLPHPGHVLTRAMKDVVLRYKTMKGFYVPRKAGWDTHGLPVELEVEKELGI
                     SGKPQIEEYGVEEFVQRCKESVFKYESEWRRMTERLGFWIDMDDPYVTFHDQYIESVW
                     WAVKTIWDKGLMYHGYKVVPYCPRCGTALSSHEVAQGYKEVEETSIFVRFKVKGEENV
                     SLLAWTTTPWTLPSNVALAVSADYDYAYVERDGETLILAKDLVESRVKGDYRIVRVVK
                     GSELVGMEYVPLYTYENPEAPAYMVVEGDFVTLSEGTGIVHIAPAFGEDDMRVAQNNG
                     LPIIQLVDSQGRFDDRARDWSGMFVKDADPLIVQDLKARGLLFEEMDYTHSYPYCWRC
                     DTPLLYYARGSWFIRMSDLREQLLANNAKISWYPEYIRDGRFGNFLENAIDWAVSRER
                     YWGTPLPIWVCEECGHEHAVGSVEELKSMAKSLPDRLELHRPYIDQAVLACPECGGDS
                     KRVPEVMDCWFDSGSMPFAQWHYPFENAQAFSAAFPADFITEAVDQTRGWFYTLLSIS
                     TLLFDRPPFENCVVLGLVLDEAGLKMSKSKGNVVDVWKLFDDHGADAVRWYLYTVNAP
                     WNPTRFYEDGVIEAKRKFMGTLWNVYAFYVLYANIDEFDPSQYHVAPEDRSVIDRWLL
                     SKLSGLVGEVTEAMDCYDITAAARRIEQFADDLSNWYVRRCRRRYWASEMTIDKQAAY
                     LTLHETLTTIAKLIAPFTPFLAEEIFTNLEPGSGSVHMQDYPKADEWARDVDLERQMD
                     LARQVVFLGRAARNEVNIKNRQPLLKVVVGVTPEEKKDLRLVEDLVKDELNIKRLEYA
                     QDMSEFLSYRVKPRYDLLGPKHGKLMKAIAEGIAEADASQIAGKVLKGREFGLVLSDG
                     TEVSILPEEVSIQTVAAEGYYVQSEGGRAIALDTVITEELKLEGFARELVNKIQTTRK
                     EAGFDVSDRICTVISGGPDSMRALEVHRDYLMKETLSVSLAHGEPEGEYVREWDVNGE
                     KVAVGVRRAS"
     misc_binding    complement(136202..136463)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        31014 a        43114 c        31715 g        30729 t
ORIGIN      
        1 aatctcgagc gcctcttcga gttcgtcttc ggtgagcgcc cccacctcga gcaaataatc
       61 gccaattcgt ttgtggccgg gccgcgtcaa agggcatcag gcctccccat cagttgacta
      121 cgagtaacgt tctacgtaca ttttcgtatt ccttctacat tactccataa atgcagaagc
      181 ctcccttata tcaggaccaa ttatcctcaa tttgcctctt agtggtggca gatggcagaa
      241 accctatcca gaatctcgtg cgccagcacg agcttggaca ttgcaggaaa tgtctcgggc
      301 ggtgcgcccg ggcagaggat tgtgacagtg ttatgatcgg ccccgaaagg agagtcctca
      361 ccgccgacca tgttggcgac tatcatgtcg agattcttgg aggaaagttt tcgctcagca
      421 ttggagagca agtcatctgt ttcagcagca aaacccacga gtacgcgctc tccctttgca
      481 ctccccagct cggccaggat atcaggcgtg cgcgagagct ccaaagaacc gaagcccgat
      541 gactttttaa tcttgctctc gctgaaagaa gccggcatgt agtcggcaac ggccgcagcc
      601 gcaacgacca cgtccgcctc ttgcgcagca tcgatcgtcg catcccgcat ctggcaggcg
      661 gtttcgactc tgaccatctc acacccctcg ggcgccggaa ggaatacagg acccgaaacc
      721 aaagtcacct tcgctccccg cacagccgcc gcgcctgcga tcgcataccc cattttccct
      781 gatgacctgt tggtaatgaa ccggacggga tctatgggct ccctagttgg tccggcggtc
      841 acaaccaccc gcagtcccgc aagtgacggc cggcccgtcc ctgcacacac atggagaacg
      901 aaatcgagaa ttctctccgg ctccgcaagc ctgcccgcgc ccacatcgcc acaggccagc
      961 ctgcccgact cgggcccgat gaacctatag cccagcgccg aaagggtctc tatattccgc
     1021 tggacgatgg ggtttgcgta catgccagag ttcatggccg gcgctataac ccggggggca
     1081 cgcgtggcca tgatcgtcgt agtaagcaag tcatcagcta tcccagatgc gaccttcccg
     1141 atgaagttgg ccgttgccgg agccaccacg aaaacatcag cggcttccgc aagggatacg
     1201 tgcttcacgt tccactcgcc aggctcggca aacatgtcta cgcttacagg attggctgtg
     1261 agcgtcctga aggtcagagg cgcaacaaac tcgcatgctg cctccgtcat gatcacatgt
     1321 acatcaagcc cttgcttgac cagcatgctc gcaagaagcg aagctttggc cgccgctatc
     1381 cctccggtca cccccaacac acacgtgcca gtcacgggcg atcacactcc taaaaaagcg
     1441 gggcacgctc caaatagtca tcggtaggct tcaactgttc cctgccgcta ggttgttcca
     1501 cccagccgtc atatttcttg ggtatgccgg tttcgccagg gctaggccta taatacgtca
     1561 cctttccctc ggctatctcc cgcagcgcgc gggttacagg cttttcagga ccatactcta
     1621 ttacgtcctt ctgcgccgac attatcttcc ttgcccgtct ggccgccatt accaccagcg
     1681 tatacctgct gtcgctatga tccatcagtt cttccaatga gggtcgaatc actcgagtag
     1741 cctccttatg catgcggcgg cgccgccgcc gcacgtacct tgtaaaagcc cccttcatcc
     1801 gccatccggg tctgtggcgt cagggaggct caacaccata ttccctcaga atatctgcga
     1861 ttccatctca aaaccgcgga taaaatgctc acagtcgcat ctgcccaccc gggcccgctc
     1921 cgcgactata atagaccgga tctgctctgc ggcctgctcc aaatcatagt tgagaaccac
     1981 ataatcatat ttcagcacgg caggtatctc ctctatagcc ttcctaaggc gtcttcttgt
     2041 cgcctcaggc gaatcagcgc cccgctttgc cagccttgcc tctagttctg caagggacgg
     2101 cggcatcaga aagatggtaa ccgcatcagg catcttggcc ctcacttggt cagccccgcg
     2161 tatctcaatg tccataatca catcttcccc tgcatcaagc tttccctcga caaagcttcg
     2221 cggagtaccg taaaggtttc cacaaaactc agcagtctcc aaaaaggccc ctgcatcctt
     2281 catggcaagg aactcctcgc gcgtcaggaa aaagtagtgc acgccgtcta tctcgccctc
     2341 ccttgggcga cgtgtagtcg ccgaaacaga gtagaccaga tcaggaactt tactccttac
     2401 ggcggcaagc gctgagtttt tgcccgagcc cgaaggcccg gagatcacaa tgaggttgcc
     2461 acgaattctc ggaacctctg taagccgctt gagcatatcc gtacccctaa tcacgcccgt
     2521 caacatctgc ttccttcatc tccactcgat gagccacagt ttcaggctga acagcggaca
     2581 atactacatg gccgctgtct gtgatgatta ccgccctcgt cctgcgcccg taggtagcat
     2641 cgatcaacat gccgcgtttg cgcccttcct ggataatcct tttgataggc gcagactcag
     2701 gactcacgat cgccaccagc ctgtttgcgg ccaccatgtt tccgaagcct atgttgacaa
     2761 gcttaatatc cacggttccc cacctacttc gccagcttgt tcaggagggc ctccttctga
     2821 cggacgccaa gaccctggac ccttcggttc tcatctatgc cgatttcctc catgatctga
     2881 cgggccctaa ccttgcctat cctaggcagc gactccagca agtatgcaac gcgcatcttg
     2941 ccaatgacct cgtcatcagc gtttgcgaga acctcctcca aggacatgtt gccctttttc
     3001 agttccgccc tgatctgagc cctcttgctc cttacctcct gagctttctt gagcgcctca
     3061 cgtttctgtt catctgtcag ttccggcagt gccatttacg cgcacctcca ttaattgttg
     3121 gcttacggca gcgtcaagag ccttactcga cattctgcac ttgttcgcgt actttctcca
     3181 gttcggcctt gatctccact gccaaagccg aaatgaatga atcctgagat ttcgctgcga
     3241 tagtattagc ctcgcggttc atctcctgta ttataaaatc cagcttcttg ccgcagtccc
     3301 ctgactccga aattgtatca cggaactgcc ttatgtggct cttgaagcga accgtctcct
     3361 cagtaatatc gcacctgtca gcgaacagcg ctacctcagt cgcaagcctg gcctcgtcaa
     3421 cacggacgtc ccctatcatc tcgcttaccc ttcgagcaag cttcgccctg taagaatcga
     3481 ccacctcagg cgcgcgacgc ccaacctcgt ccaccctcat ctcaatcatt tctattctgc
     3541 ctgcgatatc cctttccagc cttgcccctt cttccttcct catctgcaca acctggtcga
     3601 tggcatcgga aagcgccggc tgaaccatat tccaaaggat atctacatca atcttcgcct
     3661 cagaaagggt tatcacatcg ggcagccccg agaggacgtc cactgatgat atcggggcaa
     3721 gagcaagctc atgggcgagc ctggccagac cctctgcgta ggcgcgcgca aggcacgtat
     3781 ctaaagcgac agttcgccga ctgccgtcgt gccaatctac cgacaccgag acttcgatcc
     3841 tgccgcgcgc aattctctcc gcaacaaggg atcttaccct ctcctcaacc ggagagaact
     3901 ccctcggcat ccggagcgag acatcgaagt atctgtggtt taaagatctg atctcaaccg
     3961 tgacatcgcc ttcgggaccg ctgtttgcag cccgcccata cccggtcata ctcatcagca
     4021 tatatcaaag ttccatcctt ttccgtctag cgtgcagctc attgtactac acaaacacca
     4081 gctgcatcaa gtattcactg caaaatgcca gcccaaacgg gatgctttta ggaacctagt
     4141 attccactca gacaccgaaa atcctgctag gatatgcaat atccctgcgg tttcatgtgc
     4201 cttggcccgc tttattcacc ctgaaaacag tacatcgccg tctgatggcc ctttgtaccg
     4261 ccctgaccgc agaagccagg aaagtagacc agccggagag gaaaaggacc aacacccaat
     4321 ccaccaccga caactcgcag gtccccaaga atgcacggcc cacgtggtga tggacgccta
     4381 acacttgaag aacgactgaa atcccgaccg caccaagcag aaatctgttt gaaggccgcc
     4441 tgcccgattc aagagccgac ctctcctcca ctcgacatcg aaagacatag accagctgcg
     4501 acatgaccaa agtggaaaaa gccattgtcc gtgccgtatc aagatcgtgt ccgagaaaag
     4561 cgccggccga tatgtagcag gcaatggtgc aaatgctgat gaagactccg tccgcgagca
     4621 ctttcagccc caaaccgtgg gcgaacaggc tctcactggg acatctaggg gctcgttgca
     4681 tcgtatttgg gtccgtgggg tcggcagcca gggccatagc aggcagcccg tcggtcatca
     4741 agttcatcca caggatctgt atgggcgtca gcggcaacgg aagcccgccc gctacagcca
     4801 gaagcatggt tagcacctcg cccacattgc acgccaagag gtatctgatg aacttgcgga
     4861 tattatcata gatcgacctg ccctcctcga tcgccgcgat tatcgtggcg tagttgtcgt
     4921 ctgacagcac catgctcgac gcctctctgg ccacgtcagt cccgtttttc cccattgcga
     4981 tccctatatc ggcctcgcgt atggccggag catcgttaac cccatcccct gtcacagcca
     5041 ccacgtgacc ttgcgctctg agagcccgaa ctacccggag tttgtgtgca ggcgccaccc
     5101 tggcaaagac agctacctcg gaaatcacct cttcaagttg ccgatcgctc atggcgtcca
     5161 gttcctcgcc tgtgagaatc cggcgcccgc tctccatgat tcccacctgc ttcgctattg
     5221 actctgcagt cgcagcatgg tccccggtga tcatcaacgt cgcgatcccc gctttcttag
     5281 ccagagcaac tgagcgtgcc gcctccggac gcggtggatc gaccatgccg accatcccca
     5341 ggaatgtgag gcccgaaaga gcatgagact gccccacccg gatctgctcg atgaggttcc
     5401 ccggcgccga ggccatggcg agcaccctca tcgacttcga tgccatggag gaattgcgtg
     5461 ccagcacggc accgtcaaac cggcggtcac gcaacacgat gccgccttcc acatgtcccc
     5521 ccacataacg gccttcgcac agttcgagga ctacctgggg cgctcccttc acgaaagtaa
     5581 ataccccgga aggcccactg caagccactg ccatcatccg ccgctcgggc tcgaacggga
     5641 tctctgcaac ccgtgtaaag ctgcgcacct tttgcataat ctcccggcca cccttgtatg
     5701 ccatggcaag cagagcgcct tcagtgggat ctccgacaac atcccagctc cgtccaggcc
     5761 catagccata accccgtccc cgtccgcgcc ccatggagcc ggtcgtcctc tcctcagcac
     5821 tctgccgcac gaggcttgca tcgttgcaca gagcagcaca aagaagcaag tcctcaaaaa
     5881 tcccatcatt taaagggtcg attgtttcgc cgccttccaa gaattcgccc tcaggggagt
     5941 accccgaccc tgtcacctga atattccgtc ctatcaaatc gatctccacc acagtcatct
     6001 ggttctgagt caaagtgcca gtcttgtcgg cgcagatcac ggtggcacaa cccaaagtct
     6061 ccaccgccga aagtttcctg acaatagcct tcctcgcgct catcctctgc acccccagag
     6121 cgaggctgac ggtgactatt gcaggcagcc cttcggggat cgccgccaca gccaagctca
     6181 ccccggcaag aaacatgagc tcaataggtt cccctctagc aaccccgagc ccggcaacga
     6241 gagcgcatag aaagagacat cccgccacca accacctgcc gagatgcgag agcctacgtt
     6301 ggagaggagt atcgccatca gatacatcct ctatcatccc ggctatcctc cccatctcag
     6361 tctccatccc cgtggcgaca actaagccta tcgcccgccc ccgcgtcacc acagtccctg
     6421 cgtgcagcat gttcctgtgc tccacaacgg gcgccgattg ccgcacaatg caggaagcat
     6481 ccttcttgac cggcctcgat tcgccagtca ggacagactc gtccacctct accccgtagc
     6541 tctctgcgag cctcgcatct gccggaattc tgtcgcccgg gtttaagatg atcacatctc
     6601 cgggaaccac atccaccgca tcgatctgcg tggcgcgtcc gtctctgagc accgtcgcct
     6661 tgggcgccga aagcttgcgc agagcttcaa gagctttttc tgccctgaat tcctggacaa
     6721 agccgagaac cgcgttgagg acgactattg cgagaatggc cacgacgtct ctgtactcgc
     6781 ccagaaaggc tgatacagcc gtggcaccga gaagcactag aaccatgaag tcctgaaact
     6841 ggtttaaaag aatcaccagc ggcgactgac gccttcccgc ccttatctga tttgggccgt
     6901 acgccccccg cctcgcccgc gcctccttag acgtgaggcc ggacgaggcc gaagtctcga
     6961 gctccctctc aaccacccca gcgctgacgg agtgccaagc gcgcaactcg gacattgcca
     7021 acaccacctc cacaccgtgc catcttccgc acgagcagtc tcgtccttta ttcctattgt
     7081 cctttgaccg ggttcataca cgggtgctat actgaaaact gcattgaaag gggcgcagat
     7141 catgcagata gatggaatta cactctatgc cgttactcgg gagatcgaaa gagagttgac
     7201 cggcaccaga atcgataaga tccagtcgcc atcgcacgac tgcatcacgc ttgatcttgg
     7261 gcgtggacgc gcaccgtcgc tcctgatatc cgcctcgccc acatctagca ggatatgcct
     7321 gacagattcg gtcccccctt ccctgccgac ccccaccgcc ttctgcatgc ttctgcgaaa
     7381 gcacttagtc ggcggcagag tcgtatctgt ccgccaggat gggctcgacc gaatcataat
     7441 tatgcagatt gaagggtggg cagacaccga tccttccgac aacaagctcc tcatagccga
     7501 gcttacaggt cgcttgtcca acatcatcct cgtccgtatg gacgggcgta ttctcgatgc
     7561 agcacaccga gtggacacat cacgcaacag atacagggaa atcacgccgg gagcatcata
     7621 cgttccgcct ccgcccatcg aaaaagctga tcccttccgg gtgacagccg aagagttccg
     7681 cgccatcatt caaaggcagt ccgcgcagat ccgcgcgcga gccgaggctg acgccgcgtc
     7741 caagcgtcgc ccaagcaggc gcaaggatag cgttgccaat ctcatctcat ctggctttgc
     7801 aggcattggg ccgaatcaca gcagagagat cgcttggcgg gcaggcatcg acccggaccg
     7861 ttcagccctc gagctccatg cggatgacat cggcctactg tgggcgcagt ttgaagattt
     7921 tcggcgagca gtcaaggtgg gcgacttcct tttcgaggca tccttcgacc gtcactccgg
     7981 caagcctaca gcctgctgtg tttggggact ttcagggctt tctggggagt atcgcgtaga
     8041 aagattcgca tccgcctccg agatgatcga cagatgctac aatcgcgccg aagaacgcga
     8101 aaggctggaa ttaaccagaa gcaacatcac gcacaaggta gaggccaggc tagcaaggtc
     8161 ggtgcgaaaa ctcgaggccc aacggcggga tctggcggag gctgaggatt catcggcctt
     8221 ccgcagaaaa ggcgaactcc taacagccaa catccactcg ttagggcgcg gcccattgag
     8281 agaagatcgc gtgcgcgtcg tggattacta tgaccccgca gcgcctaccg tcgagatcga
     8341 agtcgacccg agcctgtcag cgtccgagaa cgcacagtcc ttctttgcac gctactccaa
     8401 agcagagagg gcccgaaaag cggtgactgc caacattgcc gccaccgaag aggaggtggc
     8461 ttatctcgaa tcgatgctgg ccatggcgac aatagccgaa acagaagaga cattgaacgg
     8521 tatcactgag gaactcgccg ccctcggctt catacagccc aggcagggac gcgggagcgc
     8581 gcggagagcg aaaaagaaac acaaagagcg gctgccgatg cccaccagct tcagagccgc
     8641 atcaggcgat gaaatatacg tggggaagaa caacctcgag aacgatcatc tcacaacgaa
     8701 gctcgcccgc ccacatgacc tgtggttcca tgcaaaagac atacatggtg cccatgtagt
     8761 agtcaggcga aggccgaacc aagaagtgcc agatgagacg ctgcgcgccg ccgcgttgct
     8821 ggcggcctac tacagccaag cccggatgtc gtcgaacgtt gcagtcgact atacagaggc
     8881 tagaaatgtt cgcaagccgc ggggcgcacg gccgggaatg gtaatctatg aggcccacaa
     8941 gaccatatgg gtgacgccat caagggagga actctccaac gccctcggtt tcaacgggga
     9001 cacttgtccc gggaatgagg attaagcccc tccctccggt cggcgccacg accgcgccaa
     9061 gccatgccga atccctgata agcctgtcca gaacagtcct ggcggcccat cccaccagag
     9121 gacggggacg gcattcctct atcctcatgg gcagaatctg cgcagagacg attttgctgg
     9181 gcgaaaacgt cacgcgagct atcatgcttt ccacagtgtc gggctttgtc tgatcgaaca
     9241 cgaagttccc tagggagtag aatatgatcc cgccgttgta cacctccaca ggctgtagca
     9301 cgtggggatg atggcccagc accagactgg ctccggcatc gatcgacatg tgggcgatct
     9361 gcatctgcct tgtgttcggc atgcgcgcat actcctcgcc ccaatggtag gagacgacaa
     9421 catggtctgc cacggcccgt gccttccgta tgtcctggac gatcatcttc tcatccattg
     9481 gcgcaacccc gggactatcc aggcccgcac gaaaactcct tggataagaa taagtccaga
     9541 atatatccgc aagctccgag tagcccaaaa acgcaatcct caccccgcct gcgctgacaa
     9601 tcgcaggcga acgcgcctca tctaggtcga acccgccgcc gcacacagct atcgagctgt
     9661 ccgccaatgc agcaagagtc tcggcaaagg cggcttcatc gtaatctagc acatgattgt
     9721 tggcgacagt gactacgttc accccggcct tcgcaagctc ctgcgcaaac ttgggatcag
     9781 acctgaacca tatgcccttg cccgggatgg gtcttccgcc cacggcaaga ctggtctcaa
     9841 gattgacaaa ccccgcatct gccgaagaga atatgggggc aacagcactg agcggccaat
     9901 ccgcccctcg gcgttcgcag acagccctca ctcccctgtc gaatatgaca tcgccggcgg
     9961 ctgcaagagt gaaccgagat tgcagagact cgcaggccgt acgctgccag acatcctcga
    10021 gcctagatcc atcgctgcag ggataggccg cccgaccctc cgaaaagcgc aaccacgtac
    10081 aatcaggagc gcaccctgta cagatcacgc atgaagccag aacggccaaa agcacctcaa
    10141 gcgccgcggc ccgtcgaacc gccaaatcga cccggccggt tccgtccgcc tttttcgaac
    10201 ccccgtttcc catcatagaa accgccccgc tatcttgcgc cgcaccgccc atcccctgaa
    10261 ggctccctcg acctaagcag tgcgagagcg gcccgcatgt catcaggcat cttcttcgcc
    10321 tcgatcctga ccctctcccc agccacagga tgagtaaact ccacactgcg ggcgtgaaga
    10381 gcctgagcct caagacccag ttcgcccgca acacctccat agacaggatc acctgctaca
    10441 ggcctaccta tatacttcat gtggacccga atctggtgtg ttcgacctgt ctccagctca
    10501 atcccgacca gagtatagtc accaaacctc tccaaaacag taaacctagt caccgccggc
    10561 cttccgtcgg gcctcacagc tcgcttcttc ctgtctacgg gatggcgccc caaaggcgca
    10621 tcaatgaccc cgcagtcctc ccggatgttc ccgcgcacaa tagcaatgta attgcggtct
    10681 atgtcgcccg atgccagctg cgacgcgaga gcaagatgcg ttgcgtcgtt ctttgccacg
    10741 ataattgcgc ccgatgtgtc gaggtcaaga cggtggacta tcccaggcct gagctcccct
    10801 ccaaccgtcg acaactgagt gcccctaccg agcagagcat tgactagagt gccggtagac
    10861 ctgcccacac cgggatgaac gaccatcccc cggggtttgt tgatcaccat caggtcatca
    10921 tcctcataga tcacatcgag ggcgatcgac tcaggcgcgg gaactcccgg gcacaggggt
    10981 tcaggcggcg gaactctcac ttcgacagca tcgccagcac ggacgcgata agaagagcgt
    11041 gtttcgacca agccattaac agtgactcgt ccatccgaaa taagcttctg aatataggaa
    11101 cgcgaaggag caactgactc cttcgcgaga tacagatcga gccttgtgcg atcatcagcc
    11161 tccccgggac tgaagaacag aacctgcatc atctaccctc gcacagacga tttcgacact
    11221 gcgccgtcag tcgaagcctc ggcgaccgac tcgggatact tcgacttcac atataccatg
    11281 ccaaatccca ataccgcaag gacgatgcat agcatttggg tggccgtcaa cggagcaaaa
    11341 tagtcgcgaa ccccgactct ccagaactca actataaacc tagcaacaga gtaaagccct
    11401 atatacgccc acattacttc gccttcaaaa cgctttctgc cttcccaagc ccgaagaatg
    11461 aagaaaataa tgacactgta gattatcgcg tagatctgcg taggatgcct aggagctccg
    11521 tccgttccgc atgccaaagc ccatggaaga tccaccgcaa ccttcccgta gcagcatcca
    11581 ttgagaagac agcccagcct tcctatggca tagccaattg ctatagcggg cgcgaccacg
    11641 tcagccaatt tccccaaagg aatctcgcgc ttcttggcca aaaggacccc tgcgagcacc
    11701 ccgcctgcaa gcccgccaaa gaaggaaagc ccaccatcgc gcatccacaa gatgctgacg
    11761 agattgtcga gataactagt gatattgagg agcacataga aaatcctggc gcccagaatc
    11821 gacgcaatgc aggcgtacag cgcgaggtcg atgatatcat cgggatctat ctccttcttc
    11881 ttgccttgcc agattgcgta gagggcgctg acaacgaagg caacagccaa acatgtgccc
    11941 caagagtata cgggaaactt cccaacatta aacaggatcg gatgcatcag ttactcctcc
    12001 ttacactggg gtcgcgcgcc atacccaaaa ggatcagtgc gactcccaca acgatggcgc
    12061 tgtctgccag gttaaacacc ggccagaagc cgacgtcgat gaaatctatg acacttccat
    12121 acttcaccct gtcaataagg ttcccgagcg ccccgcccag cgccagcgcc attccaaacc
    12181 tcatcgaccg cctgtgcctg ggaattctgg gatagaaaag caatatcagc accgctgcga
    12241 caattgcggc aacaatcgac aatatctgct tgttctggag tattccaaac gccgcccccg
    12301 ggttgtgcac atttgtgatc tgcagcagcc cgccaaggca ctcgatattg tcccatggcg
    12361 ctatctgcct cgtcacaatc gtcttagtta tctggtcgag aaggataacc catgcacttg
    12421 tcaggaaaag cgacacatgc ctccacccca ataatctctc tcaagtctga actcaattct
    12481 agcacacgaa atgtgccccg taaacactct aacatgccac gcaactacta aaacgaatcc
    12541 acgtcgccag agttcccggc ctcttcatcc tggggactgt tggcagtgcc atactccgct
    12601 agcgcatccc agatatcctc tccgtggatg ccagggtcgt cgtaggcgcg ggcaggatcc
    12661 ctgccgccga acggaggcct cacaacttct tcctcgatag gcctaggacc gctccttccg
    12721 gaaggtgaca gagaatcggc tttgcactga gcgcaactgg tcgtccacgg tatcgcgtcg
    12781 aggcgctcgg gaggaatatc ccgaccgcac tggtcgcatt tgccataaga cccatccgcg
    12841 atacgctcca gtgcagattc tatttgggcg actatatccc gcccgtggtg tatcaagccg
    12901 aggttaatag atcgcatctc cagcgcagaa gcgctatctg ccgtgtgatt atcgtaactc
    12961 gaaagctcgc cgctccactc cgattctggc gtgtcgaaac ccgcagtctc ctcaacatcc
    13021 cgcaccctcg attccgcctg cgccttcagt tcaataagcc tgctcttgca cgaatcgatc
    13081 ttttcgggtt ccatctgcgc acctcccgcg tcatacgccg ctatccttca ctattttgac
    13141 gatcttggcg attaggttgc ctatgattgg aatattaagc gagatgacaa cggaaagtgc
    13201 atataggaat agcgcggtaa ggacggacgc ccctatagca atgccgaatc ccctagccat
    13261 tccagcaagg aagttcgacc aaatgacccg ccgcggattg cgcataaact cgacatactc
    13321 cgcaacgcta gaatcctcga ggcgccgaga gagctgcatg atttgggaca tgagggccct
    13381 gacttcgccc ggccgccgct tccttcgcac agatcacacc tcgcgaagga tatagtgccc
    13441 acgtccgaac ggttttactc gtattttctt gtaactcccc ccacgaaccc ctccgcatcc
    13501 cggtgcgtat cctctaacat ctcgagatag gcctcgagca ttgccttgaa cctggccctc
    13561 gcagcggcct cctgagcgta gagatcctcc aaacgtttct gctgagcacg gaccctctcc
    13621 tccgtccgat cgagcacggc agcagcttcc ctctcagcat tttccacaat gagcgaagct
    13681 ttttcctccg cgttgcgcct agcatcttca gctgcctttt gagccatcag aagagtctgt
    13741 ttaatcgttt cagctatgtc ctcatcgcgt tcttccggct catactgggc ctgctcggaa
    13801 aggagtcgca gcttctcctt gagttcagca ttttcgcggt acaactgatc gtagcactgt
    13861 cccaccagat cgagaaaatc atcgacttcc gctgcattgt aacgcccaaa tttcgtttgg
    13921 aactgcttag aatggacttc aagtggcgta agcattccga ccctcctctc aagagaatgg
    13981 cgcgtttgcc gaataacgtg tcaacatgag ggaaacacgc cctcgcttgg tttccccgaa
    14041 aacttcctcc acaacaagcc tgcctctccc gcgagagact acgacatccc cttcactaac
    14101 cttgacagaa ggagactgaa caatctcccc gtttaccttg aacctgcccg ccttgatctc
    14161 ctgggcaagc cttgacctcg atgccggaaa tccagcgcct gccaccgcgt ccagcctcat
    14221 cgaagccacc gtggccctga tcttcttcgg aacaaccccg ggaaacacca cctctgcggg
    14281 atccagttcc accagtctgg cgcctgatcc ctctagctct cgctcattgc tcccatcccc
    14341 gaagacagat gcgagcacca caccctgcca ctcgttgccc ccatagaaca cgtctccgat
    14401 gtggtcacgt ggaatgccca aggcccgaag ctcggcaatg gccgcatcag acccggatcc
    14461 cccatcaaga ctgagcccga cgccacgcag cccaaagtag gatatgcccg cattgaaaac
    14521 ctcgtcgaga tacccagaag gcataatcac aagcagctta cgcgccgcac ccctgtatcc
    14581 cccgtaaaat cgaggcgtca catccccaat tgacgcaagc acttcggccg catcgtcctg
    14641 ctcgttcgga tcaataaatc ccagccgcac aggaacacct gactgggctg cctcgcgcgc
    14701 ggcgcggagc acagcagaat cgtcgtacat gcaagcacct ccatacaacc ggctcgcagt
    14761 gggaaatgcc ggatatcacg ataactcgcg ccgcctaggc ccgaatatcg ccgtgcccac
    14821 cctgataatc gtcgcgccct cttctatggc cacctcgtaa tcaccggaca tccccatgga
    14881 cagctcgtcc attctcacgt tggaaatcga aagctttcct agatcctccc aaagctttcg
    14941 agcccgagca aaacatgggc gagcatcctc cggatcatca gaatatgggg caatagtcat
    15001 cagtccgcaa acccgcaggc gctcgtattt cgccatttca agcaaagcat cccgggccat
    15061 ttcaggcggg atgccgaact tcgtatcctc gccggagaca ttgacctgga gaaggagatc
    15121 tataacgcaa tcagccattt cacatctgcg attgagttcc tcagccaacc tagcgctatc
    15181 tagagaatgt atgagagaga aaagctccac agcctgctta gccttgttcc tttgaagatg
    15241 ccccaccaga tgccaactga catccgcttc cacaagcggg atcttctttg cagcctcctg
    15301 caccctgttt tccccgaaat cccgaatccc tacggccact gcctcagcaa tccgttgagc
    15361 ctcgacattc ttggttacac ccaccacagt aatctcagag gggtcgcgac ccgcgcgccc
    15421 cacagctgcg gcaatcctgt cttgaagtat cccgagatta tcggcaatcg acgacacaaa
    15481 acccacctca tcctaggcta cgtattctcc gaattctaac ctattcctcc ataccttccc
    15541 cacccagaca gggaatgtac cgccgctatt tcggcatccg cgcactacgg ccgccatcgc
    15601 cgagctttac cctatctcct tcgctcaggc cagcgaccac ggcttcggtc ccatttgaac
    15661 caatgacctc gacttccttg aacctgaacc ggccagtccg aaaaagataa actccagtca
    15721 ccccgtactt gcttgttaaa gcctcaatcg gaaccaccgt ccccgagacg tagccggtga
    15781 tgaagtcgat atcctccgac cgcagactcg tgagggttgt cgcatatcta tctagagcca
    15841 catggaacgc ccaagttcct tctcccatgt caggacggtt catagcgccc atgacagtgg
    15901 caggaacagc ctcgagggcc aaccgtggga accgaaccca tactcgcgcg ccgaccgcag
    15961 gtacgcattc cccaggaaac tcggcaaaga caaggagatc agtcctgaag ttctggactt
    16021 cgcggaagac tgcagaaccc gccgccacca gatccccctc agcccgaacg tgaggaaagg
    16081 catcatagtc agcgggatta attagaagaa gatcaggatt gcgcgggtta aatatccctt
    16141 caagcccatc caaatgggcg ctgatcagag aagcgtcgca gcttcgaagg acagccagcg
    16201 cgcgtcctgc cgccgcgtca gactggcgct ttgcttccgc tatggcagcg gcggctgcct
    16261 gagtgtcaaa gacgatccgc cccacctcgg cctcagccgc tgcctcccgc ttgtggagct
    16321 gctccagttc ggaatggacc ttgttcgcgt tcttagcatc ctttcgatcc acatacgcct
    16381 tgagttcctc ttccttgagc gcaatctgga cccttatagc gagcagacgg tctttttccc
    16441 tgccctcgcg cagagccgcc tcctgaacga atttgtccaa ctcttcctcg atgcgcagag
    16501 cgttcgcctc cacagatgac gaaatcgctg tatcacgcac ctcagcaaca gcagtgccgg
    16561 ccgcgacctt ctcgccatct ggtaccagaa aatggattat tccgctgatt ggagtgatta
    16621 cgacacgttc atccctgact attatggcct taccccgaga ggaaagctcg atcgaaccgt
    16681 ggcgggccac tgcagattcg gagaacctga tcactaggcc cggactgacc attgcgtaaa
    16741 gagaatagga aagcaccgcg accacaacaa cgatcacggc aattgcgaag gacttctgcg
    16801 tgcgcttagc gggcgtccgc tcttccaccc tagcccgacg cggttctgga gccctcatgc
    16861 cagctgatga cctccccgca gcccccaccg tcacgcagac ggcgggccga tgcatcggcc
    16921 ataattatat cacagcccat gcacgagaaa ctcatcagca acgaacgtgc catctccagt
    16981 ctgaagctga tacacatatc ccgaaaaacc ggtggacgca gtcgcggaaa ggactcccaa
    17041 ggttctgaaa ctaatctctt caccaagagg aggagcattc ttcccaagtc tcggacgttc
    17101 aagcatgaga agcgcgtctc cggccttgac atcgttcgcc gccacgacca cgtcgaccgg
    17161 gcccctcctc acgtatagct cgtgttcgcc agtcagcaaa atacggccaa gacgtgtgcg
    17221 gatgtggcag agctgctcac cctcccacag attgcgcctg gccacgcaaa gaagatcggt
    17281 ccaacccgat ctgcctaaga tcttgtattc cccaagatca tcgccgccag catcggaatc
    17341 gagcatggca ttgaagaaca caagagctac ttccagaatt cggccagccg agtccttgag
    17401 gaaaacagtt gtatcctcgg aaacacagcc ttgcatgtaa catcgcctcc agcaggcgaa
    17461 agtataacat cagtggcacc gtctttcaag gggcaatccc cctttactat caacatcctt
    17521 cgtatatatc ctgcccctag atggacccga tcaacatgta tgttatggat aatatcgggg
    17581 taataccctc attcccggtt cacgtaaggc gcacacgagc atacccagat caattatcgc
    17641 cttgtccggg catcgccacc tcagcctggg cttcccgttg ccgttcaccc agtatactca
    17701 gggtactttc gcataacggc attccccagg cgatacccgg acaagatttt gccttcatct
    17761 tcccgggtct gactcgctga cccttgcgag aaatggcgat agaccgttgg gcggtctcga
    17821 cagctccttc aagttttgat ttggaatcgg tctccaatac taatccaatc aagagcagta
    17881 ttcttgtaga tgctacgatc ccaacaaacg gccccaggat catgcattga agatggcgct
    17941 aaatggggac agcgatcaca gcgtcagtcc cgcgtcatcg acgaggatta catctaggcc
    18001 gatcttgcgt atccgatccc attctatgac cagctccccc ccgcgcccca aaaatcccag
    18061 aacttttccg ccgctgggta tcacaaagct gatgactttg cctgtgtcca tgtctatctc
    18121 cacgtcgaac atgaagccca gtctctttcc atcgttgacg ctcacgactt ccttgaccct
    18181 cagttcagat gcccgctgta tcacgtgcgc acctcctgaa aacgaaaact ggccctccac
    18241 atagtatgcg ggagccagct gcaaggtgct tgttccagtg gcctatcttc atctcttagc
    18301 ttgatcaaac ctgctcaggc cgaggagcct gcatatccac tccggcaatt cccagttgag
    18361 ttgcgcaagc gtagcacgct gagtagccct ggatgcatga tagagggccg cttccttttc
    18421 ggcagccgtt ggagcaaaca gaagagacct cgttgcgaac tcaaggccga taggcctgtt
    18481 gccatcttgc ctagcctcta ttatgcatcc ctctgtcacg tccaccaagc aaagaggggg
    18541 aaacagaatg caccaccagt ttgagccggc agccggccct atagccgctg taagcgccag
    18601 gtagcttcct gaaggcaacg atatctggcc gtacgatatc gcagggaact gctccatacc
    18661 caaggtaacg cggacggaat gtgagaatcc cgcccgtgca acaacttcat tcgccattcg
    18721 ttggatccga ccgaggttgg cccggatgcg cgcttcagcc tcacgggcat ccttcgcgct
    18781 ctccagcaag gaagaggtct cgccaagaat ggcgtctcga accgccagct tcaaacagct
    18841 gcttgcgctg tcatctgccg gtgccacgac gtgaagtctg atcagattgg acgtgttgta
    18901 ggctagcctg gcttcacccc aagccgcatc agattgggca gccagaacca cggcgcacag
    18961 cgtgaaacag aataacacta gtccacatgt tattgttctg gcgactattt cctttttcgt
    19021 agtcggcacc ccctcttggg cttatgagac ccgagttaca tcgattttcg catctgtcga
    19081 agcgcgtttt tttcgagcct tgacacctgt gcctgcgata tgcctatctc gtccgccact
    19141 tccatctggg tcttcccttc aaaaaacctt agcttgagta tggtctgttc cctcggcgac
    19201 agtctgagca tggcctcctt tatagaaatc ccctcaagcc agttcgcgtc ggtgttcttc
    19261 tcgtccgata cctggtccat gacgtatatg gggtcgccgc catcgtggta tattggctca
    19321 aacaaggata tcggctcctg tattgcatcg agtgcataga ctacctcttc acttggtatt
    19381 cctaaagctg cggcgatctc gtttatcgta ggttctcttg cagtttctga cattaacttg
    19441 tcgcggacct gcagggctct gtatgcaata tcgcgaagcg agcgggatac tctgatcggg
    19501 ttgttgtcac gcaggtagcg ccggatttcg cctattatca tcgggacggc atatgtggag
    19561 aatttcacgt tttgagacag gtcaaagtta tcgatggctt taatgagccc cacgcaacct
    19621 acctgaaaca gatcatcaac atattcgccc ctgttggtga atcgctgtat taccgagagc
    19681 accagccgca agttcccgtg tatcagtttg gcgcgagctt ccttgtcgcc atcgcgcacc
    19741 gcttcgagca gttcacgcat ttttgagctg gatagcaccg ggagtttcga ggtattcacg
    19801 ccgcagatct cgaccttgtt aagcgccata tcggaacaac tccacattct cacattcgtg
    19861 ttgctaacag aagtattgct ccgacagcca aggtttattc cgcccgcatg aacttaattg
    19921 gcgatatttt cacaaaaaat cgcccccacg cggatccgcg caggggcaag cacctggtaa
    19981 gaatggacta gggtcattct accctgatca tctgccttct caacttggta agaatcctct
    20041 tctccagcct agatatgtat gattgagaaa tgccaaggag atcagcaacc tgtttttgag
    20101 tcttttccga tccgtccgaa agcccgaatc tcaactccat tatccgcttc tccctccccg
    20161 acaagctcga gagcgcctcg ccaaggagtc tccggtccac ttcctcctcg agggcctgat
    20221 aaactagatc atcttcagtg ccgtagacat cggaaagcaa gagctcattc ccgtcccaat
    20281 ccacgttgag aggttcatcg aacgagactt cgctccgtat cttgttggtc ttgcgcagat
    20341 acatcagtat ctcattctct atgcatcgcg atgcgtaagt tgcgagcttg atcttttttt
    20401 cggggtcaaa tgtattaacc gctttgatca gtccgatcgt gcctatggag acaagatctt
    20461 ccacgccaac tcccgtgctt tcgaatttcc tcgctatgta gaccaccagg cgaagattgc
    20521 gttccacaag ttcacttctg acgcagatat ctccagtctt cagcttttcc atgagaaatg
    20581 cttcttcatc caggctcaga ggagggggaa gcatttcgtg actcccaata tagcggacct
    20641 gggagtcctc aacaattacg aaccaaccaa tggccttgat tatgaagagt cgaattgccc
    20701 acatgatgct ctctaaacgc tttctcatga acaagcctcc caggagcgct caacccgcgg
    20761 tgaggatctc ggggttgagc agcgcgcggt attttccgtt agaacacaag ctagcgttat
    20821 gcacgcagat aatggcccta tctgtcgaaa tctccttctt tccgtctatc accctaactt
    20881 cactcggccg aaacccaacc ataagccctc tggtctggcc cacagaagag taaggcactg
    20941 ccctgaaacg tgttgaccat ggagactccg caattgcggc agctgccgcc gcaaaatcgc
    21001 cgtcgctaat ctccaacata gccttgattg tatgaggcag gataggctca atagcggagt
    21061 attccacgat tattgccgga acgcccgata ttgggtctct cagaagattc ccggtgtcta
    21121 taagagctgt tagcgagact gaataccggt cgaaccttat cgagataggc acgtaaaaca
    21181 agccctcccg cattccgcga tggactgccc cccatcccgc ttcggccact aacatcaccg
    21241 tggcaacgga tgcaatgaaa gacaacggtc tgctgccgcc agcagcggaa taagccgccg
    21301 tagccgcacc acctgccata acggtgagga ggtatcggta gaagagggcg acgatgagct
    21361 cgcgcgcgtc cattccagga aaggcaatga ccaatgatgc gccagcggcc aagagtgcaa
    21421 cggtcagtga catgggcgat gacaggatta tgatcccgag atcggagaga atcacaatgg
    21481 ccacaaatga tgaagcagat agtacggaag ctcgcatcag ttttgaagcg cgtgttgaac
    21541 gcctgcttat ctgccccact gcccataaag tcaggaaatc cagaacaaat gttcctcccg
    21601 caagaaggag caaagaatcc aggtatatgc ggatcatact ctacgccccc tcattgtatg
    21661 attgtatgca gttgatcccc ggattcttct cacaatgtga atgtagaagt caaggaaggc
    21721 tctgtgtgtt gaagtgtaac actgcgctcc gtcgaaccgt gtcgggttgc gccgacagga
    21781 caaacgaatg attccggtat tacgcaaaac tgccttgccc accatgcact gaacaagctg
    21841 caggcagggc agttggacat cttgttcaat tcgtctgaaa tgatcataca gctggatatc
    21901 agaaggcctc tacttgaagc caagccctat agccaaacgc aaattggcac ggcttcggag
    21961 gcccgtccgc tcccggctcc tgtacagctg aacgcggggc gcccctgcct cctggcaaat
    22021 cagcgccccg ctacacgaac tctcctcatt tcaatctgct ccggttctac cttgcccgtc
    22081 tcaggaacgg aggaatatcc agatcgtcgc ttccagcgag aggcttgatc tggactctat
    22141 cagtcctggc cttcagaggc tgctccgcct gttgaacatc gaatccagta gcgataacgg
    22201 tcaccttaag ctcgtccccc attgaatcat caatgactgc gccaaagatg acaagcgcat
    22261 ccgggtcggc agcctccgaa actatctctg ctgcctcgtt cacctcaaat aagcccatat
    22321 ttgaagaccc agtaatgttc atgagaacgc ccttcgcccc gtttatggaa gcctccaaaa
    22381 gcgggctggc cgtggcggag cgagcggctt ctgcagcgcg gttttctccg cttgcaatgc
    22441 ctatccccat caatgcagaa cccgcgtcag tcatgatagt ccttacgtcc gcaaaatcga
    22501 ggtttatcag tcccggaacg gttatgaggt ctgaaatccc ctgcactcct tggcgtagca
    22561 cgtcgtccgc aatcctgaac gcatcgagca tcgatgtctg gctctctgca acctgcaaca
    22621 acctatcatt gggtatgaca ataagggtat cgaccttatc tttcagctct tccgttccgt
    22681 ggtccgcctg cgccattcgt cgcctgccct caaatgaaaa cggttttgtt acaacgccca
    22741 ctgtgagagc gccttgttcc ttagcgatct cggcaatcac cggggcagct ccggtacctg
    22801 tgcccccgcc cattccacac gtgatgaaca ccatgtctgc gccatccagc gtttctgcaa
    22861 gcaaatctcg gctctcttca gccgccttct taccaatctc gggattagcg ccagctccaa
    22921 gcccttttgt aagcttttcg cctatctgga tcttccgggg cgcattagac atgcgcaggg
    22981 cctgggcgtc agtatttaag gccacgaatt cgacaccttt taatcctgca cttatcatcc
    23041 tgttgatggc gttactcccg cccccgccaa ccccaaccac tttgatgttg gccaactggt
    23101 caacctcaag ctctagttca aacacctcgc atcccccttg ccgctcgcct cacgaggaaa
    23161 atatatctcg aaaccattgt cgcacttcgc tagcgaaacc tccggacttt ggccgccggc
    23221 cgtctgcaga gggaatcacc gcagattcac tccaaacaga tttgactaac cccaccgcag
    23281 tagcgtattg agggttgtcc aacgcttcct cagctccata tacccctaca ggaactccga
    23341 ggcgcgcagg aagccccaga acctcctggg caacctcgac aacaccctgc atcacagaag
    23401 cgccgcctgt cacgactaca cccgctggca gcagatccct gtatctcgcg cgatccagtt
    23461 catctccgac aagctccata gtttcggtaa ttctcgcctc aataatctga caaagcttct
    23521 tcctggatat gttcgtgtcc gctccgcatg attcgttttc gccaaccatg tcgggaaggg
    23581 ccgacccgta gttcagcttc aatccctctg aatagcccac gggcaatctc ataaccaacg
    23641 caatgtcgtt agtgacgtgg ttacccccca caggaataga tgcaacatga tgcacgccac
    23701 cctcaatgca gactgcgata tcggtggtcg cccctcccat atcgatcacc agggcgccct
    23761 gttccttctc agccagcgtc agaaccgcct cggcagcggc tatcggttga agtatgacat
    23821 gttcaacttc cagtccaatc cgcctcgcgc tcctgtgaat gttctgtatc gcagtagccg
    23881 acccagtgat aatgctggcg tcgacctcca accttattcc cgtcatcccc acaggcttgc
    23941 gtattcccga gcatccgtct atcacgaatt cccttggcag gacgtgcacc acttctctgt
    24001 ctggcggaat cgcaacgaca cgggcagcct ccaacacgct atcgacatcc tcctgggtta
    24061 cctcccgatc agacctaccg atagctacta ctcctctgct cgcaatagaa gagacatgcg
    24121 tgcctgacgc gctcgccagc actgaaacag ccgcgcaccc tgacatttct tcagcatgcg
    24181 ccacggcatc agcaatggcg gcagccgccg aatccatgtc aactatgact ccacggcgca
    24241 gtcctgcaga cggaccactg cctactccga taatgttcaa gttgtagtct tcatctcttt
    24301 cacagacgac cacgtgtatc tggttcgagc caatgtcaag ccctactttg atatcgtttt
    24361 tgcccaatac agttcccccc gccgactcca cctgaccctc tcgctttgtt gcatgattct
    24421 agaatcgtac ccacttaccc tctatcatag cgggagattt ggaagaacca caacccccct
    24481 cagtcgatct cctgacgtta gcaaagaaga cgccagtccc aaacgcatac tccgatcgct
    24541 tatctcgatc ggatcaccgg tttcgcttca actcgcaaat ctatgtaatc tgccgacatt
    24601 tcctgttctc taactgaagc caagatcgaa tcgagaaccc gcacctgtga ttccatgtcc
    24661 cgggcgccgc caatgaatac cttgattccg tctgatgttc tgattattat gtcttcacac
    24721 gaagagacgt ctacctcgga tatgttggga atcccctgtt tgagcagggc ggcgcctact
    24781 ctggcaccca cagacacctc gtatttgggg ttcaccggct gccctacctc aacccaagtc
    24841 ggagcaactc caacgatgag aggcacatcg ggatcggtga tcgcctcaga tatgcctatt
    24901 gcataccctt cttcatcgac gtctataaaa taacctccgt atgggagata cccggcagtg
    24961 ctgcgttcct ctattgcaat gctgatagta tcaggaagga tcctgtacac ttttgccgta
    25021 cgtatcctag gcgtggccaa gagttttctt tcgatctcac ctgtcggcac caaaaacatg
    25081 ttggtattct ctggaatacc tgcaatgaga aggacctcgt aatccgacag atagtttgat
    25141 cccgacactc taactgccct aaccagaaaa tacggggagt tgagaaaagc tacggttgcc
    25201 agaaccagaa acagcagcac aactgcggga aatgatatgg ccggccggga ctcggcgtgc
    25261 ggctcctcct ccaagccagg catatcagac atatcagaag agtcaggcaa cgcttccacc
    25321 cccctggcag caaccagcct gaccccatta taacacgatg tcaccagcgc aaagaatcac
    25381 ccaaagaaaa ttcattgctc ggccacagcc gcactggcag aatccgttac tatctccact
    25441 tcagcgccgc aagccgcaag cttcccttgg atatcatcat agcctcgata tacatgttcc
    25501 atgccatcca atgtcgtctc tcccctggcc atcagtccgg ctatgatcag cgcagctcca
    25561 gcccgcaaat ctggggccac aacccgcgct ccggtgagtt cgtttactcc tcggattatc
    25621 gccgttcgac cgtctacgcc tatatgcgct cccatcctcg caagttcgtc cacatatctg
    25681 aatctattgc tgaatatgtt ctcagttata gtagatgtgc ctcttgacac agttagatac
    25741 gccgcgatcg gagcctgaag gtctgtggga aatccaggat atggcatgct cttgacggca
    25801 ataggccctg gcctgtcgcc catagcaacc cgaacgcagt cttcggtata tatcacggct
    25861 acgccggttt ccgccaattt ggcggccagc gcgtccaggt gttcgggaac cacgcctgtg
    25921 atagtcacat caccccgcgt ggccgccgcg gcaatcatgt atgttccggc ctgtatcctg
    25981 tcgggcatta cggaataatt ggttgaaccc agttctgata cgcccacaat ctttatagtg
    26041 tcagttcccg ctcccctgat gtccgcccct agccgagtca ggaagttctg ctcgtctacg
    26101 atctccggct cccgcgccgc attcctgatt atcgttgttc cagacgccag acacgctgcc
    26161 gccattagat tctcagtcgc gcccacgctg ggaaaatcga gatgtatatc agtccccaca
    26221 agtcgggacg ctctgaccat tatgtaccca tgctcctccg aaacctgggc gcccatggcg
    26281 gaaaggccgc gaaggtgcag atctattggt cgggagccga tggcacaacc tcccgggtat
    26341 ggcatcttca cctctccgaa cctggcaaga aggggaccca acacctgtac ggatgcgcgc
    26401 atcttcctca ccaacggatc cgggggggcc gccgacgcga ttgtcgtcgg atcgactgtt
    26461 acctctgtgc ggcctgtaaa ggagactcta gcgcccaaag cctcgaggag ctgacacatt
    26521 gtctctacat cagtaacccg aggcacgtta gaaatcacgc atttcccaga cgccattatg
    26581 gacatggcaa gcagtttcag gcaggagttc tttgcgcccg gcgtcgctat gctgcctctc
    26641 agttcacgcc cgccgactat cctcaaagtc tccatctcag ccacctcgcg cgtcttaatc
    26701 tataacgaca ccaggcctaa ccaccgacta ctctgatctc aggaacaagt tcgactccga
    26761 atttctcata aacggtccgc ctcgccacat tcatcagtat caacacgtcc cgcgcagtcg
    26821 catcgcccac gttcactatg aagtttgcat gaactcggga tatctctgcc ccgcctacgc
    26881 gaagcccttt gagacccgct ccatcgatgt accgaccggc ccctctcccc gaaggattct
    26941 ggaatacaca cccggcgctc ggaaggtgca gaggctgctt tattctgcga tcctccatgt
    27001 attcgctcat ctggcaacgt atgctttccg gatcgcccgg ttccaacacg agcacggcct
    27061 cgcaggcgat gagatctccg tgggaaagcc gactcgacct ctgggcaaac tgcatctcct
    27121 cgcatgactt cactgaaatt gacccatcaa gcaacatggt ggtcacttcg cgagtaaagt
    27181 ctcccacagt gtgactgtac gcgccagcat tcattgccat tgccccgccg agtgaacccg
    27241 gtattcccac tgcccattcc agcccggcaa ggccccgctc ggcgcaggca cgggccaaag
    27301 ccggcatgct gacgcctgcc attgcgcgaa cccgttcgcc gtcaaaactg acgctgtcca
    27361 ggcccggacc tatcctcact actacgcccc gaattccttc gtccgcaact agaaggttcg
    27421 taccattgcc cagaacaaaa aggggcaatc cctcgttcca ggcccatcgc aataccacag
    27481 acaggctctg cagatcgcga ggctgcacaa gcatatcggc tggaccaccg atctgaaacg
    27541 acgtatattt cttcattggc tcgttcagcg caattctccc gttgaacgac ttggcaagct
    27601 gccttgcttg ttcatcggtg accatgctcg tcatcaagtt tccaccgccc atcatgcgat
    27661 agcctaaagc cagcctcagg acgagccagc tcctcggcga agcgccgtga cgatctgtag
    27721 atgtcgcccg ctcccatggt aacaactgtg tcgccagggc cagtcgaatt tctgagaaag
    27781 tcatccagag cagccctgtc ggcaagatag gtcacaggct ttccctcata cgccgcaatc
    27841 ctctctgcaa ggtctctccc ggaaactccc ggtataggcg actcacctgt gccctcatag
    27901 tagatatcgg taatggctat gacatcggca agactgaacg accgggcaaa ctcgtcgcga
    27961 agatacttcg ttcttgtgta gcgttgcggt tgaaatacga ccacaagccg tccctcggct
    28021 gccttccgca gagcggccag agttgccctg atttccacag gatggtgcgc ataatcgtcc
    28081 accaatgtca tctccggcgt tttggccagc acctggcatc tgcgctcagc acccatgtaa
    28141 ccggcaagcc ccctgcttat gatcgaaaaa tctattccca gctgatcgca gacggcaacg
    28201 gcagctatgg cattactcat gttatgcatt cccgggacgg aaagctcaat ctgcccgagg
    28261 catgctccgc ggcgcataac agaaaaacgc gatccgaagc cgagctgcga atagtcggcc
    28321 gaagtgtaat cggcctcgga agagatgcca tagtagatga ttcgccggtc aagtccctcg
    28381 gcggccctca acacgttgtg gtcgtcagcg cacagaaccg caaatccccc aggcttaatt
    28441 cttcggagaa attgggtaaa accctccatt attccttcaa atgtttcgta gtaatcccga
    28501 tgatcatcat cgatgttggt tacaacagcg atttccggat cgagttttag gaaggatcca
    28561 tacgcctcat ccgcctcgac taccatgaac tccccctcgc ctgcatggcc tccgctcgga
    28621 gtccatggca gaccggctcc tactgccacc gtgggatcga ggcccgcttc gttgagaata
    28681 tgcgcgatca tcccgcttgt cgtagtcttt ccgtgtgttc ctgttacggc tattccccgt
    28741 cgaggcctca tgatttcgcc caagagctct gctctcgaaa caacccttat tccacgctcg
    28801 tgcgcagctt gaagttcggg atcatcctga ggcactgcag ccgacgctac aaccaagtcg
    28861 accccgtcga tccgtgatgg atcatgaccc tgatatacat cagcgccaag agccgccaga
    28921 tcctcgagat gaggagagta gcgtatatcc gacccactca cctcggcccc atggccaatg
    28981 agtattctgg caagcggact catgccactg ccgcctattc ccacgaaatg aacgcgcatt
    29041 gaactcatat gacccgcacc tttcccgaca cgccacgctc ccgcggacga tgctattgta
    29101 tgccttctgc cccctcggag agcatgtcct gcccatagaa tgcatcttgc tgcggcgcgc
    29161 ccagatgctg aatgagtcga ccacgatgcc tccggacaaa tcacattcaa ggtttgatct
    29221 tatcgtaatg tgccgtctat cgcgccgcct ttaccacaca atctgccacc ctcgcagcag
    29281 catcgggtct tcccagattc cgcgaagccc tggacatgct ggccagtaca ggccttgacg
    29341 acaaaagcga gtctatttga cgtcccagaa cctccggttc aagctcgctg tcgagtatca
    29401 ctctggcagc acccctggct tccatggcgc gggcattttt ctcctgcaca ttgtcgggga
    29461 cgtgtggatg agggacgagc actgcagcca cgccccgtgc cgcaagttca gcaagggtga
    29521 tcgcccctgc ccggcaaatc gccaggtcgg cgcaggcaag ggcctggggc atattgtaaa
    29581 tgtatggcat aataataatg tccccacgat ccgacctgcc tattccgtca gaggaaatac
    29641 ttgccctaat gcctgattca gccgcaagcg ccatagattc ctcgaattta tcgctccctg
    29701 tagccagtat gaattgtgcg tcgtgcttcc ccgatagcga cggaagagct cggataaaag
    29761 ctgtatttac cctcctggcg ccttggctcc cgccaaacgc caacacaatt ggacgcctcg
    29821 gatccagccc aaacgatgcc gccgactcct ctcgttgcgc cgacattatt tccggacgaa
    29881 tcgggttccc ggtaaggact gaacgttcgg gccgagctag atactgtttc gactcttccc
    29941 agctcacggc cactgtcttc gcccatcgtg aaagcaagcg gttggtagca cccggaacca
    30001 cattctgctc atgtataaca acaggcaccc ctagcatcgc cgccgcaagc atcaccgggc
    30061 ccgaaacgta tccccctgtc cccacggcaa cgctgggcct gaatttcctc acgatagcta
    30121 tggattggaa aaggcctgcc accgcgcgtc ctgcggtagc gaaagtgtcg agtgagagcc
    30181 ttctttcgaa tcccgaaacg gtgatcgtct ggaactcgaa acccgccttg ggaacgagat
    30241 ccccttccag gccgcgcttt ccccctacaa acagaatatt ggcatcagga tatcgcgaaa
    30301 gcaactccct ggctatggag atggcggggt atatatggcc ccccgtgccg cctcccgcca
    30361 acaagaatct cttctgcgcc gatttctcac tcttcaacga gctacctccc ccgacctaga
    30421 gaaccctacc ctatctctgg ttgacatgct tcgatacatt gagcagtatt cctatgcccg
    30481 ccaacatcgg cacgagcgac gagccacccg aacttatcag cggcaacgtg attccggtaa
    30541 taggcatcat tccggaaacc acggcgatat tcatcatagc ctgcaaagtg accattattg
    30601 taatgccagc agcaagaagc gacccaaacg agtcaggggc attgagcgat gttcgaaaac
    30661 cccgaaggcc aaatatcatg taaaggagaa caacggaagc tgcccctaaa aaccccaatt
    30721 cttctccgat gacggcgaag atgaagtcgg tgtgttgctc gggaaggtaa tgaaacttct
    30781 gcctcgacag ccccagtcca acgccgacaa gccctccgga gcccagtgcg agaagagact
    30841 ggattatgtg gaatccggaa tcaagagggt cggcccacgg atcaagaaac gaagtcagtc
    30901 tcctgagcct tacagggtcc tgccttgcaa aaagatatac ggcgggaatc gccgcggcta
    30961 cggtggcgat cagcgatgcc atgttcgatc ccccggcaaa gagcatgaac catcccactc
    31021 ccgcgattcc aatcgctgtg ccaaggtccg gttctttcag aacaagaaca gagcaagccc
    31081 cgataatcat catcggcagc aagaaacccc gaaagaagct cttcgttcta gcctgggaag
    31141 ccgaaagata cgacgccagg aacacgacca atgacagctt tgcgaattcg gaaggctgca
    31201 cccgaatgga ctcgactccc aaccatcttc gagaccctga cacgacaatg ccaaccccgg
    31261 gcaccagaac gaggacaagc gctcccagtg aaagaaccat aagaaggcct gcaagcctct
    31321 cccatacatg gtaatctatg ttcatacaca taatcattgc tgcaattccg attatcgccc
    31381 agagggattg gcgcttcaga taataatagg ggtctccaag ctcgtgatag gccgttaccg
    31441 agctcgcgct gaagaccatc acaatcccaa tgctgagcaa agccagcgtt gcgacgaaga
    31501 tgacaagatc aggaagccct ccgcggtcgt ttaacatgcc aaaatcaccc cgtacgccta
    31561 aagcgccgca aatcctaaag ccgcaagaaa cgcagccact atccagaatc tcaccacaat
    31621 cttcggttcc gccactccct tcagctcgaa atggtggtgg ataggagcca tccgaaacac
    31681 acgctcgccg gtgagcttga agtaggttac ctgtatgaca tcggacatgg cttcaacaac
    31741 atatatcccg cctatcacga gcaagaggag ctccgtccga gtaatcaccg ccatggaagc
    31801 gagcgcagcg cctaatgcca atgatccggt atcgcccatg aaaacagatg ccgggtaagc
    31861 gttccaccag atgaacccga ggcacgcccc gcccacagcg cctgcgaagg cacttgcttc
    31921 tccgtggccg agcgccgatg atacagcgac atacgcgaat gaagcgattg ccatggtccc
    31981 tgcagcaagc ccatcgaggc cgtccgacag gttgacggca ttcgtcgagg caaccagaac
    32041 tagcatgaca aagggtatat accacattcc aagatcaacc acgtgtccat gagtaaacgg
    32101 gatgataatg ccagatccaa tccctggatt cgaataagcc catgccccta aaatcaaccc
    32161 gaacaccagc tgaaacagga tcttctgcct ggcacgcaag cccaaagaac gacgagcgac
    32221 gatcgaaatg taatcatcaa gcatacccac aagcccacag cccgttgtaa caaaaagcgc
    32281 ccatggcagc atagcataac tgggcgaagc cgctgctgaa cccacaagta cagagactat
    32341 gataatcacc ccgcccatgc tgggcgtgcc cgactttttc aggtgcgact ggggtccgtc
    32401 atcccgaaca acttgacccg ctttgagttt ccgaagattc tttatggtta tcggacctat
    32461 caacatggca acagcgaatg caatccctgc tgctatcgat gcgcgaacca caacttccga
    32521 catcatacct caccactgtc tggagccttc agaaggccgc ccacaatctc ttccatctgc
    32581 atgccccttg accccttgac caatacgacg tcccccggac gggccattct tccgagaact
    32641 cgcaacgcct ccgagttact gctacatgcc tctgcctcca cagaagaacg cgccgcagcc
    32701 ccagctgcaa gagcagccat tccgcccaca ctcaccattc cggcaacgcc tgcctgcgcc
    32761 gcatacctgc ccacatcctc atgggcaacc gcactggtct cgccaagctc gagcatgtca
    32821 ccaaaggcca gaatcactct acgccccctc gcatattgca acgcggcatc gattgcgcac
    32881 tttaccgatg caggattggc attgtaggca tcgtttatca cagtatagcc ggcaggagat
    32941 gacgcaaact ccattctcat cggggaaggg ctgaatgaag caaggcccgt tgccattacg
    33001 tcgggactga gccccaatcg ccagcccacg gtgagggctg ccagggcatt atacacatta
    33061 tggaggccgg gaagcggaac cgaaaaacgc actccgtcca ctctatgtcc ttcaataaca
    33121 aggctgaaac tgatcgagtc ggcctccggt tcgatactct ccgctctcac agagcattcc
    33181 tcagacaggc catagagcca ggcgcggcct aaaaccaacc ctgccatacg cctgacaaga
    33241 gcatcatcgc cgttgagaac cgcaaaccca tcgggaggga gcgcctctat cagctcggcc
    33301 tttgcggcag ctatgttctc cttcgatcct aaaaggccca catgcgcgta accgatattg
    33361 gttatgatgc ccacatctgg gcatgcaatc ctcgatagcg cagctatctc cccagggccg
    33421 cgcatcgcca tctcgaccac gactatctgg tgttcaggcg tcatttccag cagcgtcagc
    33481 ggcagaccga tttcgttatt cctattgccc gaggtggcaa tcaccttgaa tcccgccgcc
    33541 aacactgcgg ccgtcatatc cttcgttgtc gtcttcccag cgctgcccgt aacccccaca
    33601 acagttgggt caatgctcat cctgtgacgc cctgcaaagg ctccaaggcc ttcaagaaca
    33661 tcgtcgacta ctatgagccc aaattgagct ggcaaaccct gcccaggcac gggcaccctc
    33721 gacaccactg cgccacagca tccgccagca gctgcgtcta caaggaaatc atgtccatcg
    33781 actcgctcgc ctggtaaggc aaagaaaagc gcatccggtg ccgccactct gctgtcaatc
    33841 acgaccgagc tcaccctctg atccgggtct ccctgagcca ggcgcccccc catggcatag
    33901 gccacatctt tgagcgtgta atcaagcatc catcctcagc tccctcaagg ccgcacgagc
    33961 ttcttctctg tcatcaaaat gaaccgtctc gtcccggaag atctggtatg tctcatgccc
    34021 cttccctgca ataagcacta catcgccacg gcgggcacac cgcactgctt cgcgtatggc
    34081 ttccccgcgg tcgaggatta tcttccattt ttcccgcccc tccagccctg ccttgccttc
    34141 gacgccggcc tcatgctccg acatcccctg gacaatctcc ctgacaatgt ctgccgggtc
    34201 ttcaccgcgg gggttatcag acgttactat ggaatagtca gccatcctct gcgctatggc
    34261 gcccatcagc ggacgtttcg atcgatccct gtcgccgcca catccgaaga cgcatatgac
    34321 ccttccatcg gaaatatcac gggcggtcgc tagcacgttc gcgagggaat cgggggtgtg
    34381 agcataatcg acgacgaccg caaaatcctg gccctcgtca acagtttcga accttccggg
    34441 aacacccgga aaatccgaca acgcgcgagc agcctcccca aggggcaccc ccactgcaag
    34501 gacagcgccg atggcggcaa gcgcatttga aacattaaat gcccctatga gcggggaaca
    34561 cactcccaca gactccattt tcgaagacgg tccttcactt tcccgccaat ggaccgtgta
    34621 gcgtgtacct ttcctgccgg ttactacgtc aacagcgccc aaatgtcggc acccgcggag
    34681 gccttccctc cccaaagcag aataagtgat tatttcggcg ccagcctcct gtgcagcgtc
    34741 gaagatggcg caatgatgcg ggtcatcgat gtttatcaca gccaccttcg gccacagctt
    34801 gccctctctt gctccaaact tccccaagga gcgaaataat gacgccttgg cgtccacgta
    34861 gttgtcgatg gtaatgtgaa aatcgaggtg atcatggcct atattggtaa aaaccccaac
    34921 atcccaatcg accaacgcaa cccttccaag agctatccca tgggaagacg cttccatggc
    34981 cacatgtgtc cttcccgccc ttaccatttc atacaagata gcctgcacgt cggcagcctc
    35041 gggagtagtg ttgggcccgg gctccaaacc cttgccggcc atgtaaccca ttgtccctat
    35101 tagccctgag ggacgtccag actcgctcag gatgtgcgaa atcatgaagg cggtagtagt
    35161 cttgcccttg gtaccagtca cccctatcgc tgtgagttcg gacgacggat cgccataaaa
    35221 cactctagca atttctgcca aagcggccct cgtgtccttt actctgacaa cactcacgcc
    35281 ctcaggcacc tgtacaggtt ctgacacaag tactgcagaa gcgcctctcc tgaccgcctc
    35341 gtcgatgaag cggtgcccgt cgaatcgcgc cccgacgaat gccgcgaaca gagtcccctg
    35401 tccgtccacc tttcgagaat cgtatgagag gcttgttatc tcggtacttg gatctcctga
    35461 aagctcgtgc gaaacacctt gcagcaaact ttccaagctg ttatgtggca tacacatcat
    35521 tcctcaagga atatcgtaac gatcgcgccc tttttgacat gcgttcccgg agcgggcgtc
    35581 tgtcgaacta ccaggccctt tcctgacgga tccacgccaa ggccacgggc gcccaaaaca
    35641 agggccgcct cctttagagt tttcccaatt acgtccggta cctgtatctc aacgtcttca
    35701 cgattataca gttcctcatg gccaaagtaa acgatgaccg tggttcccgc cccaacttgc
    35761 gaaccagcct taggcacttg gttcgttacg acatcgccaa tcccctcgtg cctccacaga
    35821 agaccgactt ccttaagccc ggcctcagcc tcagcccgac tttttccggt cacgtcaggc
    35881 acgaaaacac tattgcccga ccctgctgtg ccctggggca ttccaacggc gtatgaaggc
    35941 ggaatctcca tgtatctaaa gatgtcctgc atcaccgcgc ggaagaccgg ggcagcaatc
    36001 actccaccgt atttcacatc cgagctgggc tcatcgagga tgacaagcac tgcgaccttg
    36061 gggtcgcttg ccgggccgta acccataaac gatgcgatcc tcttgtcacc gtaccgtcca
    36121 cgctcgggct tttgcgcagt acccgtcttc cctgccacag cataacctgg cacagccccg
    36181 cgttggccgg agccgttgac gattacagat tggagcatcc tcgaaagctc tgatgcggtg
    36241 gaagaagata tcacctgccg cacttttgtg ggaacatgct cctcaaccag agccccatca
    36301 gcgccgatta ccttcctcac caacatgggt ctcatcagcg ccccgccgtt ggctatggca
    36361 cacatcgcat tcagaagctg caatggcgtg acggatatgc cctggccgaa cccgtaggtc
    36421 gcaagttcca ccggtcctac cttcgatatc tggtgcatca tccctgtagc ttcccccggg
    36481 taatctactc ccgtcttctc gagtagtccg aacgcgcgag cgtactggac gaatttttcg
    36541 accccgagtc ttaacgcaag cgtggcgaag acggggttgc aggaattctc gagggcttcc
    36601 atgaatgtct ccgaaccatg cccacccggc ttgtggcatc taatggtccg gtcttcaacc
    36661 ttgagatatc ccggatcgta aaatggggtt gaggcggtaa cgatgccctc ctcgagggca
    36721 gcagcggcgg taataacctt gaacgtagat ccaggctcca acatatcact gactatgaag
    36781 ttgcgcctga actcgctcgg gtactttccg tagctgttcg ggttgaatgt gggcctcatc
    36841 gccatggcca ggatttctcc agttttcgga tccatggcaa ggatgagccc acgcgtggac
    36901 ccagtctccg ctatcgcgat ctcgagctct cgctcgcata tgtactgtat tacttcatcg
    36961 atagtcagaa cgaggctatt tccgtcagta gggggcacgt atgagtggcg gccttcggga
    37021 atctcgcgac cggtcgcatc tctttcggcc actatctggc ctcggacgcc cctaagctca
    37081 gcatcgtaat actgctccag cccttccaag ccctggttgt cgatcccggc gatcccgatt
    37141 acgtgagcgg caagcgtgtc ctttggatag aacctctgcc cgctttctgc cacggcaatt
    37201 ccgggcagtg agagccgacg aacctccata gccgcctcgt gatctacctt tcgctttagc
    37261 caaaccgtct gctgggtggc ggtgagcttc gcaagaagcg catcctcccc catacccaat
    37321 acgtgcgcga gcgcctttgc tgttttggct ggttcgctta cttcagcagg aattgcgtac
    37381 acggaatcgg cgcttatgct aaccgccagc tcccgcatgc acctgtcgta catcgtccct
    37441 ctcttaggat ccaccggaac cggtcgcatc ctctgctcaa gggcaagttc tctgaagtat
    37501 ggggctttca ctatctgcac ccaaacaagg cggcccaaaa caacaacaaa aagtccggcc
    37561 atcaaaagga ggaccgagta gatccttttc cttatgatgg cggacgtcgc gtcagcgcga
    37621 tatccagtgc gagaggaagt tcgagaccgc acctgcaaag cccccatcca agtgttatat
    37681 catctaagaa cagatggaag ctgggccggg ggagccgcga accaacccgc aataagattc
    37741 gataccgctg ctactgccat ttgctgcatt atcgttgcta cgcctgcaag cacagtcggc
    37801 ggttccgaca ggccttgagt tccagctgcc actggcgcca cttccgcctc cgcaagcatg
    37861 gggcgcgcat caacaaccac aattcccatg ttgtcagggc tcgccatgcc caactccgtg
    37921 cgggcgatag cgtccactct ttccagcgag gcaacccgag ccagctccaa cgatttcctg
    37981 tcccactcat tcttaagcaa atccagcctg gcctgaacat cagaccggcg atcgcatgcc
    38041 ctcattatct cagttctctg agctatcgac ataaccagaa ggacaattgc aagaacggcg
    38101 acgatgacgc actcggccat aagccggaaa gaccgcgtat tccttcgcct taccgattcg
    38161 cgtccggcat ccccaagtct tgcgttggtg cactccatgc ctattcaccc cctaatgtgc
    38221 ctagaacttt ccttgctgct ctcagcttgg cgcttctcga tcgtgaattg cttctgacct
    38281 cttcatccgt ggggccaatt ggttttttgg tcaagacatc caataccttc ttcttgccgc
    38341 atacgcatac aggcaatgac ggtggacacg tgcacggacg ctccgccctt ttgaaggcct
    38401 ccttcacaac cctgtcttca agggaatgat aggatatggc cactatcctg cctcctggtt
    38461 gaaccctgtc tatagctgcc aagatgcctc gttctaccga cccaagttcg tcattgactg
    38521 cgattctcaa agcctgaaac gttcttcgcg ccggatgtgg cccatctcgc ctcgccccgc
    38581 gcggaaccgc agcgtaaata aggttgacta agtccccgct cgtttcgagc ggcgcagtct
    38641 tgcgtcgctc gacaatgaat tctgcgattc gcgatgccca tcgctcctcg ccatattctc
    38701 ggatgatcct ctccagctcc ccctctgatg ccgtgttgag tatgtccgcg gcagtcaccg
    38761 ggccgtctgg atccattctc atgtctaagg gcgcctcggc cctgtatgta aagcctcttt
    38821 cagccgtatc gagctgatgc gaactgacgc cgaaatcaaa caggatcccg tttggattcg
    38881 ggtagccagc ctcctccaag atgacggcca tatcgccgta attcccccgc gccaatatca
    38941 cgcgacaggg gacgccctcc aggttctgcc tagctgcatt caaagcctga ggatcgagat
    39001 ctatgcctat gagaacccca gattcgccta tcttccgcgc tatatccagg gaatggcctc
    39061 caccgccaca ggtagcatcg catatcacat cacccggcga cggatcaaga tattctaaga
    39121 cctcagacaa catcactggt cggtgagtgt agctcatctg ccctctccgt tattgctgat
    39181 ttggcccgta tgaaacgagc ttctcggcaa ttgattcata agctgtggat acgcgagaca
    39241 ggtattcctc ccagacttct gccgcccata tttcgaatcg agaagatact ccgattatca
    39301 cgatgtcccg ctcgatcctg gcatattccc gcagatgcgc cgggaccaag gcccttcctt
    39361 gtttgtccag ttcacactcg cttgcacccg aaaagaacaa ccgcgagaag gcgcgcccat
    39421 ctgccatcgt cattggcaaa gcggagagct tctcttcctg aagccgccat tcatccattg
    39481 tgaaggcgaa aagacagaca tccaatccgc gagttagaat gaaccggcca tccaatttct
    39541 cgcggaactt ggcggggatt atcaccctcc ccttatcatc cagacaatgt ctgtgctccc
    39601 caatgaacat gttggccacc actttactcc actttgctcc acttggcata tgatttcgat
    39661 gccgaaaggc attctccttc aagggtgtag aaatattaag ccaactttgt tattcgggga
    39721 caaaaaaaag agggcctaag ccctctaatt tcgctctttc actaagacaa tactaagaca
    39781 cggcttgcat gttcatgaag tcggacctca agatcgtcat gagagtaaca accctgactt
    39841 ggtcatctgg accgagtctc cttttcacga ctccctcgcg gcgaaacccg catttcttgt
    39901 aacacctgac agcccgcaga ttgtcggagc acaccctgag atacaccctt cttaggccga
    39961 ggcgttcgaa ggcgatgaac aacatttccc tgacagcaca ttcgccaata cctcggtctc
    40021 gataggcagg atccccaatc tttatgctca gctcggcatc gccgcttcgc catgcaatct
    40081 ccgtgagttc gatgtcacca ataagccggc ctgaatgatc gacgatggcc ataaccaggc
    40141 tattccgatc gttcagcatc ttcagatatc taccacgtga ttcctcaatg cttatgggga
    40201 ccccgcccat catatgtgcg agctcagagt cactgtccca ccccgcaatc ctgtcaagat
    40261 cgcgctcctc gagcggtcgg agcacggccc tttccagttg ttgcatctac gaccccttct
    40321 tccggtacga ctccacctcc caacgcgctt cttggattcg ccttgcagca ggtatgctcc
    40381 ttcaggaaac tgtacccaag gtcggccgtc atagccatcg cgagcaacat gtgatatagg
    40441 tatagacaaa tatttattat agcgtcaaaa acaagaagca cgccgttccg accctggcgg
    40501 aggaaaagcc tagactaccg cggctgcacg ataaacttga tagctgtgcg ttcttcacca
    40561 tcaatcgtca cgtccacaaa ggcaggtatg cataccagat ccagcccgct cggtgctacg
    40621 aatcctcgag caatagccac tgccttaacc gcctgattaa tggcgcccgc tccgatagct
    40681 tgcacctccg cgcctccgcg ctcacgtatc acccctgcta gagctccagc cactgaatta
    40741 ggatttgact tggcagaaac ctttaggact tccatgacat tatcctcctg gttggctaaa
    40801 tcagcgtcta ctcctaactg atgtattagc cctttcttgg aaaactcctt ctgctcattg
    40861 gcacaagttt catcgaatgg tatcatcaag ttcctccaag aatcgttgca catacttcgt
    40921 tttcccagtg gaagtatcga tttctactat gaccccgcac acaatcaccg gccccttgcc
    40981 cacctcaaac ttggagggca tggcagttat gaacttgggg atcaccgtat ccggatccat
    41041 tccgataacc gagttgacgg ggccgcacat ccccagatcc gtgatgaatc cagtcccgtt
    41101 cgcaaggacc cgctcatcag atgtctgtac atgcgtatgc gtgccaacca ccgctgatac
    41161 tctgccatca agatagagcc cgagagcctg tttttcgctg gtggcttcgg cgtgaaaatc
    41221 cacaacaatg gcgtctgcgt tggagcctat ctgctcaata gctcgatcgg cgcagatgaa
    41281 agggcagtca ataggctgca tgaaaacgcg gccctgaagg ttaagcacgg cgaggcgcaa
    41341 ggcgcccttg gtcactatgg tccatccatt tccgggcaca ccttgtggat agttggccgg
    41401 acgcagcacg cgtttttcgg tctggaggaa cccagccatc tccctgttac gccagacgtg
    41461 atttcccgtg gttattacgt caacccccgc gtcgaatatc tccagtgaca catctggtgt
    41521 aaggccgatg cctcctgcag cattctcccc attaacaaca acgaaatcga gttcttcgcg
    41581 ggatctgaga tctgctatcc agcgaagcgt gtgcctccgt ccgggcctgc ctatgatatc
    41641 cccgagcatt gcaactctca acggcactcc tccgtttctt ctttagacga ataagggcag
    41701 gttcatctgt aaacctgccc gccttattcg aacatattct caagaggctc agtttccgcc
    41761 agatcacgcg ggccgattgt acactattac acggcccccc tcgcgaaacc cgattagcct
    41821 cttctcttga gtcgtctctt tcaaatcaac aaggagatta gcgatgaact cctcctggac
    41881 gatcatctct tcctcttcga ggtcttccga cctgtccgtt acgagatcat catcgaaagc
    41941 atctattatg agcccaatca gaagcgatat ctcatcgcct gtgagggttg caacgtccct
    42001 tgtcacctgt atctgggtga tgcttccaag atatacggcg cacacctcta ccgtgggcgt
    42061 ctccccgaac ttcagaaggt ccaggtaaac agagatgctg tcaagaacgc tactgcgaaa
    42121 gccttcaacc tcatcgtctt caaggcgcct cgacaccatg aacgagaacc cgagcatctt
    42181 tgcatctggc gtgtacgaaa ctgtgcagac ctctggatac cggacgagta tggacacgag
    42241 cagtccaatg ctggccgatg atccatcgat ttccttagtt cgcacctctt tcaccgctat
    42301 caccttcttc ttcatctctc cgctcctgta acgaacttct ctttcacaga agcgattcct
    42361 gctgaccatc ttacgcatac cggacttcga tagcccacaa ttacttggcg taatcgaccg
    42421 atcttgtttc gcgtattact gtcaccttaa tctggccggg atactccagt tcctgttcga
    42481 tctttttcac aatatcccgt gccagcttaa ccgatgcaag atcatctatc ttgtcgggct
    42541 tgaccatgat tcttatttcc cgaccggcct gtatcgcgta tgccttatcg actccttcga
    42601 acgagtcagc aatttgttcc aacttctcaa ggcgtttgat atacgcctca agagtttccc
    42661 gccgcgcgcc cggacgcgca gccgagacag catcagccgc ttgaacgagt accgcctcga
    42721 ccgtctgcgg ttcctcatcc ccatggtgtg ccgcaatggc attgaccacc gccggggatt
    42781 cacggtattt tcgcgccaga tctgcaccaa tagtaacatg aggcccttcc atctcgtggt
    42841 cagtagcctt ccctatgtca tgaagcaatc ctgcgcgctt cgccatggcg acgtcggctc
    42901 caatctctga agccattatc cctgcgagat gcgaaacctc tatagagtgt tgcaggacat
    42961 tctgcccgta gctcgttcgg tatttgagcc tgcccaaaag cttgaccaat tcaggatgga
    43021 tgctatgaac tccaacctca aaagttgcgc gttctccttc ctcccgaata acggcgtcca
    43081 cttccttctg tgcacgctcc accatctctt caatgcgggc aggatgaatt cggccatcct
    43141 gaatcagttt ttcaagggca atacgggcga cctcgcgcct caccgggtca aacccggaca
    43201 atatgaccgc ctcaggagta tcgtcaatga tgagatcgat ccctgtcaag gtttcaagtg
    43261 cccgaatgtt ccttccctca cgacctatta tgcgtccctt catctcgtcg ttgggaagag
    43321 cgactacgga cacggcgcct tctgcaacct gatccgccgc gcacctctgt atcgcctgtg
    43381 taacgatctc ccgggcgcgc ctttctgcct cctcccgagc ctgcgactca gcttctttga
    43441 tcatgacagc cacatcatga gttatctctt cctcagcttc tcgtaagagc tgttcacggg
    43501 cttggtcagt agtcaggcca gcgatgcgct cgagctcctg cctttgctcc tgatgaatct
    43561 tctctatttc agcctgagtt ctgtctattt ccttttcacg ccgatcgatc tggctttctt
    43621 ttctctccag ttgatcggac ttcctgtcga gcgactcctc tttctgaagt atacgcctct
    43681 ccacacgttg gagttcgttg cgtctgtcac ggcattcgcg atcgaactcg ctccgcattc
    43741 gatgcacttc ttctttggct tcgataacag cctcgcgctt cttggcttcg gcttccctct
    43801 cagcctcttc aattatgttc tttgcagcat cctccgcaac cgagatcttg gcctcggcaa
    43861 tcttcttccg cgcaaggtat cccagataag cggagagagc cactacagct acataaatca
    43921 atgcctgaat aaatgggcta atgctaatcc accccccttt accaaaccaa tgactattgt
    43981 tgaattgtcg gtctaatcac gcattataac tgaagccgag cggtaactcg gcttcaaaca
    44041 ggcggcggca agaacagcac gcctgcggcg atgcagttcc ggagcaccaa acttgagcat
    44101 ccggctccaa cgatgattct atatgtagtt tgcagctaat gtcaagctag atgccatcaa
    44161 cgaaatagct cgcgcacaac gcgcgcaatg gtatcggatc gaaatcctcg gcgggcaaga
    44221 aatccggcta cacgtctctg tgaacgcaag cgagccttat catcgcacag atcgtcatct
    44281 tttacgcgct tttcggccag ctccaaagct acttcaaact cctcaagccc tgcagctgaa
    44341 tatgccgatc gtgcagcctc atcagccaaa tcggggtcta ttccccggcg caccaaccgc
    44401 atagcgatcc ctttcgcccc atggcgcctc tcgtcgacgc acatcgcggc ccaccggtgg
    44461 gccgcgtcaa catcatcgat gtacccgcaa cggatcatct ctccaactat gtcgtcgacg
    44521 actgcggaat cgtggccctt cttagaaagg gcatctcgca gctccttctc tgtgcgagcg
    44581 cgcactgaca agatcttgta cgcttgggcc cgggcgtcgc tatgagcgcg actgcaagac
    44641 gcgttcacgt cggaatccga atgccaaggc aaagactggc cggatgatct cgcgaccggg
    44701 gagtgttcgt ctcctggctt gccagattgc ccggagacgc ctgggagatc cagccgccca
    44761 ctgcccccgt tcatcaactt tttgatttcc tctgtccgtc cttcttgccg ctgccatcag
    44821 cgtccttgcc gccatctttg gcgtcagaag ccgccccgct gccggacgcg ggtgaccccg
    44881 caaggggaag cccaacactc tccctcacct tgacctctat ttctgccgcc agttccgggt
    44941 tagacttgag aaactgccta caattctctt ttccctgccc aagcctgaga tcgccatagg
    45001 aataccatgt tccacttcgg atgatgatcc cctgggcctc acctacatca agcagagacc
    45061 cctctttcga gatcccttcg ccatagatta tgtcaaactc cgcttccttg aaaggcggcg
    45121 caactttgtt ttttaccacc tttccacgag tgcggtttcc gatctgttca gaaccttgct
    45181 tcagagactc aacccttctg atctcaaggc gtacggacga gtaaaacttg agggctcgac
    45241 cacccggggt aacttcagga ttgccgtaca ttatccccac tttttcacgg atctgattta
    45301 tgaatatcac acatgtattc gatttcgata ttgccccggt cagtttgcgc aaagcctgag
    45361 acatcaacct cgcctgcagc ccgacgaagg aatcgcccat ctcaccctct atctccgatc
    45421 gcggcaccaa tgccgccact gagtctacca caataacgtc tacggcaccg ctgcgaacca
    45481 atgcctcggt gatctccagt gcttgctctc ctgtatccgg ttgggaaacg agcaggttgt
    45541 cgatgtccac tccaagccgc ttggcgtatg tagcatcgag agcatgctct gcatcgataa
    45601 atgcagcgat ccctccaagc ttctgagcct ccgcgattac atggagcgcg acagtggtct
    45661 tgcccgatga ttcaggtcca tatatctcag cgaccctgcc gcgaggcatg ccgccaacgc
    45721 ctaacgcgat atcaagggct attgcgccag tcggaatcgc atccacctgc atcactccac
    45781 cggattcgcc caggcgcata atggacccct tcccgaagtc cttttcaatc tgagtcagcg
    45841 ccacctcaag cgccttttcc ctgtcactca aatcccatcg ccccttccgc atttcatccc
    45901 atttgcacgt ctaattcgat ttcgtttcat ttcacctgcg cccctgtgta aggagcgaca
    45961 cgatcaagag acgtcctgca tttctctctg agcaatgcgg ggacagccca tctagagcat
    46021 accacatttc acgcataacc gcacatatgt tcggtcgtca ctcaagaggg atttcggcca
    46081 gagacgtgta gatggggccc gacggcgtaa gcttgcttga acagatatgg atagccttga
    46141 catcgacggc gcccagatag atgtcggcat tctctgagat gatcctcgat ataccagcgc
    46201 ccgcagagcg ttcgcgtgct ctgcctatgg taaggtgagg cctgaacgcc ttgtcggcag
    46261 gagcaaatcc agcatccact gtagcccggt caacggcttc ggcaagccct gcaaggcgct
    46321 gagcgccacg gtcaactcct acccacagag tcctcgccct ggcgacatcg gggaaagccc
    46381 ccgctccctg caacgtcatt tcgaacttgg catcgccctc cactgccttc ctgacggctc
    46441 cagcaagcgc aggaatgcgc gacaacggca agtcgcccaa aaacttcagc gttaagtgga
    46501 gattacgtgt ttcaacccat ttgacagacg cgccaacctt gcgcatctcg gcaatgagat
    46561 ccgccaccga atgggctgca gggcccgcta cgggcacagc tatgaatgtt cggacagtat
    46621 ccaatgccgt cccccctttg cagtatcaga ttccagatta gagccgagaa tccttcaccc
    46681 gcgtcatcgc ccgtaagggc tattctccag caaaaagagg gtgatttgtg gatgcgggaa
    46741 cgttgcccct atgtccaagc atcctcaaga tgcctcctaa gcaaatcgag gccgaacttg
    46801 cacgctcgcg tcctcacaga acgccggtca ccaccgaaca tcattcgagc agtaatgaca
    46861 gaggtcttct cagtgtctgc caggcagaag tagacgaggc ccacaggctt gtcggcgctc
    46921 ccgccaccgg ggcctgctat ccccgttacc gaaaggccga ggtccgcccg tgccgtccga
    46981 accaccccac acgccatttc cacagccgtc tcacggctta cggcaccatg gacgcgcaat
    47041 gtcgattcgt ccactccaag ctcatctctc ttcgccgagt tgctgtatgt cacgattccc
    47101 ctctccagaa aggatgaaga tccgggcaca cctgtaagca tcccgcatat gagtcctcca
    47161 gtacacgact cagcgaccga aagccttgcg ccagcagata tgaggcgcct ggcgacaact
    47221 ccttcgaggg tgtcgtcgtc gtacccatat atggacctgc ccagcctagc ctcgagttcc
    47281 ttctctactg gctcaatcaa catccaagct tccttctctt ctctggcgca tgccgtgact
    47341 ctgatctgac attctccctc gcttgcatat acggctaccg tggggttaac tccctcgcaa
    47401 gcgagatcga gtatacgttc ttccagggaa gcctcgccaa tgcctatggt tcggattatc
    47461 ctggaaacga tgacttcacc agcgccgttt cgcgttaaat aggcgcggag atacggctcg
    47521 acgccggagt tgatgagata ggccatctcg cgcggaaccc ccggaagaca gatgaccctc
    47581 ttctttcccg attcgaacac cactgctggc gctgtgccca ccgcattgcg aaagaacgtg
    47641 cccgagctag gctcgggcac gagtgcttgc ttcctgttat tcgctgtcat gcgagtaccg
    47701 cgggaggcaa agaacccttc gatatccgag aggacctcgg gactctccac gagatggatt
    47761 ccgaggacct cagcaacggt ctcctttgtc aggtcgtctt cggtgggtcc gagtccaccg
    47821 ctagtgatca ccacatcagc ccgctcaagc gcttcgcgaa ccacagtggc gagcctcgcc
    47881 ctattatcgc cgacagtcgt ttttctgtat agggagaacc cccacccggc aagcgatctc
    47941 gcgatatgcg cggcgttggt gtccaccgtc tggcccaaga gtaattccgt tccgacggat
    48001 atgatctccg ctttcatgca aaaccctccg acaggtccga gcacaccgct gtcggcctga
    48061 cagagacggc cgaaagcgga ttcgtgccac cccaatcagg ccgtctgcga gagctgggcc
    48121 ctgaaagtat ccccattcat tttctccact gacatgagtt cgcctgcagc ctttctgata
    48181 aattccgcat tggggacgag caacgacttt gcccgttcct cctgagcctg aacaagcctg
    48241 gtcacggccg tatgatattg gtctcgaggg atgtcctcct cgcttatgat ccccagatcc
    48301 gacaggccac agttgacaat cttccttgca gtttccaccg cttgttggat gtcttgtgac
    48361 gccccagtgc ttcgctcccc cagaatcagc tcttccgcca tcgccccggc aagaagaacg
    48421 ctgattctgg cttcgagctc tgccgctgtt tcgatatatt tatcagacct cggagcatgt
    48481 ctcacgtatc ccagagcttt cccgcgcgag gacactgtta cagctgccac tgaccctggc
    48541 ttgagcagct cgctcacgac agcatggcca agctcgtgga ctgcgacgcg ccggagctct
    48601 tcctcatccg gctttcgctc tatcctttcc cccatgatca ctttttccat cgcttcagaa
    48661 aagtcattca ttgtgatccg gacccgcccg gccctgagcg ccatgatcgc cgcttcattg
    48721 gtcaagcttt caaggtgagc cccggagaat ccgtaggtct ccgcagccac cttgtcaatg
    48781 tcgacatcct cagcgagcgg cttgtgcctg gtgtgtatct ccagaatatg gcgtcttcct
    48841 tccctgtcag ggaggtccac atctaccagg cggtcgaatc tcccaggtct aagaagcgcg
    48901 ggatcgagga ggtccgcccg gtttgtcgcc ccgatcacca ggacccgtac accctcgcgg
    48961 gaagcagatg tcagcccgtc catctctaca aggagctggt tgagggtttg atcatactca
    49021 aggtgcccct gatgcgtgcc gcgtactccg cctaagacat ctatttcatc tatgaatatc
    49081 actgctgtct tcttcttgca cgcacgcgca gtggagcgcg cggttccaaa cagatcccgg
    49141 acgcgcttgg ctccaactcc tgcgtacatc tctatgaagt cagacccggc cactgaaatg
    49201 aacactgcgt ctgtgtagtt ggccgcggcc ttcgccagga gcgtcttgcc tgttccagga
    49261 ggaccgctta gcaaaatgcc tctgaggggt ctgatgccaa gcttgaccgc cttttcttcg
    49321 ttgtttatga actctaaggc ttcgactagc tctcgcttgg cagtctcctg acctccaaca
    49381 tctccaaaag taagcttgga taccctgccg ccagattctg cctcttgaat gacctcgaac
    49441 ttgcgatcgc caacccgccc tgacatcatc aactcaaatg cagcgtatag accgcccgca
    49501 aagatgatag gggtcacatc gtacccatac acgatacaga acaggaaggc agcgatgcct
    49561 gtgcctatca acacatctct tatcatctgc ccacgcctgc ctttccccgg ggcgaacagg
    49621 tagagagcca tcttgcgtcg gatcggacaa ccgttccgcc ctggactgct ccagacgcac
    49681 gcgatacaac tgcgtaaagc cggtgctcgc cgttttcaag gctgacataa acattgtctg
    49741 catccacttg aacaacacaa cctgatagcc cagcctgccg agctttctcg tcagcacgcc
    49801 cctttaggtc cgagaaagat ccagtggcta tcgcttcctc tatatcgaag tgcattctgt
    49861 agtacacatc ctgcaaaagc acgtcacgcg tgtccgatat cctcacccac agtctaccgt
    49921 cggcaggcag cttcgcgacg gccgcttcta ctttcctcac agtctctgaa aggtcttcca
    49981 cgttgccaag cttcacttcc actactgcgc cataagccga acgctcaaat gaatacgatt
    50041 cgatctcgga catagaagcg aaagccctat ccattggcat tgcaacagag gtcctgagaa
    50101 acacttctcg ccctccaaaa agaagaaccg cggccaccgc cacgcttgcc agcactactt
    50161 tgatatttat tcccggtaac ttcaaggccc cgcctccttt ctggcatatg gccttttagc
    50221 tgcacggatg attataacat acactctttg caatatctct gtaagtacgt ggaaacccct
    50281 ccgtacatct acagaggggc gcaagcaggt acagcgaaat tagccgctct tcaagcaatc
    50341 agatcatctg ctgggttctt tgcgagcttt ccgcagccgc aatggctgcc gcccggagga
    50401 caccgatggc ctcccgagga ttctccgcgc caaatacata gctgcccgca acaaagacgt
    50461 tcgccccagc ccgggcagca gcacctatag tctcgtcatt tattccccca tcaacctcga
    50521 tatcgaagct caagccattc ctgcgagcca gtgccgctat ggcctccacc ttgtttagag
    50581 cctcaactat catgctctgg cccccaaaac ctgggttcac agtcatgacg agcaccatgt
    50641 ccaccatgtg caagacatgc tctatggcgc agacaggcgt agcgggattg agggccacac
    50701 ccgccctgac gcctctctgc cttatccctt ccaaagtgcg atggaggtgg cgagaagcct
    50761 ctgcatgaac agttatcgca tctgcgccag catctgcaaa cgaatctatg tgttcttcgg
    50821 gattgacgat catgaggtgc acgtcgaaag gaagatctgt agcctgtcgg atacgcctta
    50881 cgaccggctg tccaagtgtg atgtttggca caaaactacc gtccattacg tcaatgtgga
    50941 tcatatcggc gtcattggct atccctctga tctcctcggc caggattcca aaatcagcgg
    51001 aaagaataga tggagctatc tttatcaaat ccaacacctg ctttcatcgt ccatgcctca
    51061 ggctttacgc ctcgcgtcca tcccccctgc aacgcgatac gcaacactgg cgcgccgccg
    51121 gctcaaagag ccagaagccc ccatacatca agggtgcatc aattatctat actgccgttt
    51181 ctccgcctcg cgaagctctt ccaggaaact gaggtaatgt gcgtaccgac ccggatctac
    51241 gacgccgcgc tctacagctt ccttcaccgc gcaatccggc tccttgtcgt ggaggcatcc
    51301 aacgaacttg cattccgatg caagacggga aatctcggga aacacgtggg taagctcgcg
    51361 aggcgcaatt gaggtaatgt ccaaagagga gaaaccagga gtgtcggcta caaaccctcc
    51421 cacatcgagc ggaatgagcc gcacttccct tgtggtatgc cgtcctcgcc cgatcttctc
    51481 gcttatctct ccttcgacaa ggtcgtgccc gggctgaacc atgttgagaa gcgcggattt
    51541 gcccaccccc gacggtccgg caaagaccga tgtttttccg gcaagtgcag cagagagagc
    51601 ctcaagccca aggccggtcc gggcgctggc gaggatggtt ttgtagccga tgtccgagta
    51661 tagacgaagc aacgactcgc actcgtctcg cgtaacaaga tccgctttgt ttatgcaaac
    51721 caccgcatca aggcccgcat actccgacat gaccaatagc ctgtccagaa gacacgggtt
    51781 agggtcgggc tgtgcagctg ccgcaacgac tattgcctgg tccacgttgg ccacggtcgg
    51841 cctaacaagg tatgtgcgtc ggggcaacac ttcctcaacc acgccagtgc cgttttgccg
    51901 cagcatccta acgcgatctc cgaccgccac accctcttcg cgccgcttca cattgccccg
    51961 cgcggcgcac ggaactgtct cctgttcacc gtcgggcaac acccaacaca gcccgccaga
    52021 aatccttacg atgcgaccga tatcatacat tttccgtctt cacagggtat ccgtctaccc
    52081 acacttcgat cttgccgggc ccgtaaactt ccacagtgtg ccgtatccaa tccccaggtc
    52141 tgtgcaattt ggggccatag cttgttcgct ccccgtaact atcattcacc ttgatcacta
    52201 tctcctggac ctcagacccc ggctgaacct gataggtcac atcataacgc cgatacgaca
    52261 tgtctatctg gccaccgcca tcgtccgatg gagaagggga agcagacgga aacccagatc
    52321 tctgagccac aacgaggtcg atcgtggttc cgctttccgc catctggtac ggcccagggt
    52381 tctgctctat gactgtgcca tcctcgtaga ggatgcttat cctcttcctt acctcgccca
    52441 ctgcaaagcc cgcccgcttc agagtatccg tggcccgctc cagagtcatt cctattacga
    52501 gcggaacctc aggcggaggc ggtggcgggc ctatggatac agtatagttc accgtcgagc
    52561 ccctcggcac gtatgtgccg acctccggct cctgatcgac tatgcgcccg ggcctcacat
    52621 cctcatgata atcttggctt ttggcgccaa gaactaagtt cgaacgttcc agttcaagcc
    52681 gggcttccct ctcggacttg ttaaagagat cgggaaccgt caccatttca ggcccaagac
    52741 ttactatcac cttgatcggc gaatggacct tccttttggc gcctgcaggc ggatcttgct
    52801 cgataatgcg attgtactcg actttgttgt cgtagcgctt taccgatgaa tccaaagcaa
    52861 gcctactccg acgcgccagg ctttcggcat ccggcaacgt catgttaaca acgtcaggaa
    52921 ctacgacttc ttccacctga atccattgac ggaaggaata gagcgcgccc agaacggcac
    52981 cggccacgac caacatgaca acgataatcg tggtccatgt ctttgcctgg cgatctgccc
    53041 gtttaggcgt ctcctgatcg ttttccaact cgaaagcctc cccaaccttg tcaaacggcc
    53101 tggtaatgtt gaggtcatca tctacgccat tggtaatgcg cgaatggggt gtagctgccc
    53161 cttcactgcc ggaagccgtc ggaagatcct caacttgttg gccatcgggc acctttttga
    53221 cctctgtggg atgggcacgc aatatggcat tcttgaatgc cctggccgta tgccatctgt
    53281 cttgcggatc cttggccaac gccttcatca ccactcgctc gaactccaca ggcaccgcct
    53341 tattgtacac cgaaaggggc aatgcgtctt cctgcacatg cttgagcgcc actgccactg
    53401 gagtatcccc aacaaatgga agcctacccg taagcatctc atacatcact acgccaagcg
    53461 agtatatgtc tgactcggca atggcggagg ctccacgggc ctgctccggc gaagtatagt
    53521 tgacagtgcc gatcacctgc cctgcctcgg taagagcact tgccgatgcg gcgcgcgcta
    53581 tcccgaagtc cgtcacctta acgcgaccat ccctcgttat tagaatgtta tgaggcttga
    53641 tgtcgcgatg gataacgccg ctgcggtgcg catgatccaa tgcatcaaga atctgaacgc
    53701 tgtaatctgc gactacatcc acggtaagcc gaccatcacg cataatgata tcgttgagcg
    53761 tctggccgtc cacccactcc atgacgatgt agttggaacc ggaatcctcc cctacgtcgt
    53821 atacgttgac tatgttaggg tgagacagag ccgccgcgct ctgggcctct cgtctaaacc
    53881 tctccctgaa ctcagaatcc gaggcgtact gtccccgcaa caccttgatg gcaactactc
    53941 tgttgagcaa catatccttc gcccgataca ctatggccat gccaccttcg ccgatggtgt
    54001 cgagtatgga atatcgtccg cccagaatct caccggtcat ccgctcacct cctagctctg
    54061 tccatagaat cgagggctac tctgataata tcgccgccca gcggcgccgc tgccgtcccg
    54121 cctgagccgg cgttttccaa gattatcgca gcagcaactc tgttattgcc gttcacgcca
    54181 aaaccagcaa accatgcatg aggatttcca tgcggattct gcgctgttcc ggtcttgccg
    54241 gcaatatcat atccccacct cgccgctgaa cgggccgtcc ctcgtttcac cgcttgcctc
    54301 atgcctctgg caactatatc agcggatgat ggcgtcatga cggatgcaag agccctttca
    54361 ctagcaacca tgaccgggac gccgtcaact gcagaaatat cggccacaac ataggggcgc
    54421 atcattattc ccctattggc aacggcagct gccatcattg ccacgtgcat gggagtcaca
    54481 agaagattcc cctgccctat ggccaactgc ccaatggcgt ctccgctgac atcagcattg
    54541 cgcacatcaa ggttcgcagg cggtgtcggg atatccaaag gtacgctcct atcaaagaag
    54601 aaacgtcgaa gctcctcata tagtttcgga cccagcctaa gcccgagatc aacaaaagtg
    54661 gtgttgcagg agacagccaa ggcatcaact atatcgatcg ccccatgagc gcccgttgga
    54721 tcgtgaacaa cagacccccc cgccctgata gaaccctcgc actcatagac ctcattaggg
    54781 tcggttaccc cgctgtcgag ggctatggct gcaatggcca tcttgattgt ggatccaggc
    54841 gggtacatcc cgttgattgg gcgacaaaca agaggcgaat cattccggga aacgatgttg
    54901 tcccacgaca gctccaaccc tttcatagaa taccacgggc tgctcgcaac agcgaggagc
    54961 tctccagtcc ccgcattgat cacaatcgca gctcctctgc gccctctcaa tgccgactcg
    55021 cacgccttct gcacattggc atcgatcgtc aaccgaaccg cacaaccatg atcgggctgg
    55081 ccggcaaggg tcgcaagcag acctgtcaca tcgtatggcc cacgcctgcc ggaaagccaa
    55141 acatcatatg cggcctccag ccccgacttg cccagcacgg gattgacata gcctacaagc
    55201 gacgcaagcg aatccggccc ccgccatact ctcgtcgaga gcccagggcc gaccgaatca
    55261 catatcacac caccgttacg gtccagaatc ggtccgcgaa tcacatcccg ctcaaggttg
    55321 gccaaccgcg gattagcctg acttgaggag agtttatcgg cgcttattgc ctgataatat
    55381 gttgccgcaa ccaccaaaat ggcacatccc gcaatccacg cctggacaag cctgcgtatc
    55441 ctcctgttca actcaaagcg cctccatcaa tggaatgcag gcgccgcgta gagccgagca
    55501 gaattccgac gatggccagg ctagtcagca tggagctccc cccgtaactc gccaagggaa
    55561 gcgtaacgcc tgtgaggggc aatacaccca gattcccccc gataatgaca aatgtctgca
    55621 cggctatcat gttggcagca cccaccgcaa ccagtgcaga gtatcttgaa tccgcccgct
    55681 gagcgcatgc ataaccgtgc cctaccatta aaaagtatat cgctatcaag ccgatcgatc
    55741 cagcaagccc tatctcctca gcagatgccg agaacaacat gtcggtaact gcagccggga
    55801 tcaaccaagg agacccgcat ccgtatcccc gcccaagtat cccgccactg gccagggcat
    55861 agatcgactg cgttacctga taacctgaac ctgctgcatc agcccacggg ttcagccacg
    55921 tggaaaatct gacgccaaca tgtgggaaga ccctccctgc caccgccaca gcagctgccc
    55981 caatcccaat cgaaactaga accaacaatc tgtttcccgt ggccacgtag gccattgcaa
    56041 cacaggaaat gtatatgacc atggcagtgc ccacatcgcg ctgcgccaca gtgagcaaca
    56101 tcacagccgc ccacatcact atcacccttc tagcctgaga acactcccat atctcccttg
    56161 cggacgctct ggcagtcgcc atggaatcgg cagtgtgcat cttcgacaac ccttccgcac
    56221 ccgccaatat acatgtgatc ttggcaagct cagacgtttg aaaccccata tcgcccaaga
    56281 acagccatga gcgagctcct ccgcgttcct ctcctaccat caaagtgagg accagcagag
    56341 caattgaccc gcccaaagcc ggaactcccc agccctccag ctttttttga ttctgaagaa
    56401 tagcaagaac agccataaca gccgatccaa gccatatgaa ggcaagctgc cgaactgcca
    56461 agtcaggcct gagcctgtat accacaggat aacccattac tgtcgccgca cctgcaagcg
    56521 gcagtattaa ctcatcgcca tcgaaaccct tagcaacaaa aaccagatga gtcatggcaa
    56581 atggcgctga tgacacgaaa cagagcgctg cttcctcgcc ggtcagcgtc ccattcctag
    56641 caagcgacaa agagaatcca gccgtcagcg acatgagcat gactatcccc aatagcgaac
    56701 gttccgtctc ccttatggga cgcgcagtgc cttgggcact cgctggcaga ctgccgtcct
    56761 ccatcatatc actagtcccc ctgaacacgc gcgggcagaa gacgaagacg atgagacatc
    56821 cgattcctcg actcggacaa ggactacagt tatgttgtca tgcccgccgc gcgcacgggc
    56881 taatctgaca agctccatgc atgcctccct agggtcatgg aacctcttga tcgtctcagc
    56941 tatctcatta tccccaacga tatcgtgaag gccatctgtg cacagcaaaa cgacatctcc
    57001 cggctcaact tgcctctcga cgatatctac ttcgaggctt tcagaaaagc caagtgccct
    57061 cgtaatcgca tggcgttgcg gatgacactc tgcctcttca ggcgtgagca ttccagatct
    57121 gagcagttcg cccaccacag agtggtcgtc ggtgagctga ataatgtcgt ccccgcgaat
    57181 caggtacgcg cgggaatcgc ccacatgccc tactctgagc gttccctcac aaaaagcagc
    57241 caccgtcaac gtggtgccca tgccttccag ttcgtcacag cgccgtgaag cctcaatcac
    57301 actgcggttg gccttctcaa tacactgagc catgtcgaga ctgccaaggt caacattggc
    57361 gcattccgcc gagacaaccc cgattgcaat ctcagatgcc acctctccgg ccctgtgccc
    57421 gcccatgccg tcggctacgg cataacatcc atccgcggca aaatatgcat cctcgttgcg
    57481 gtcgcgcacc agaccaatat cggtagcaaa cccaaccatc acgtccctca ccccatccag
    57541 gctagcatag acaaactcaa gacgcatcat cgccgccagc atcgggcaca gcggcagact
    57601 ggccttccaa catcttttca tcgtcaagat tatccgaacc ggcatcaaag gaaacgtcga
    57661 ggaccagagc cctacaaaca cgatatagaa agaagtagag cgaggccacg cacgcaagcc
    57721 taaggatcgc cagcgccaca cggaccattc aatctctacc ccccctcatc tcgccttttg
    57781 ctcggatcag acgcccctgg acgccctgcc cacctccgcc tcagctggcc acaggctgcg
    57841 tctatatcgc cgcccatctc acgcctgata gtggtttcga tcccagcgga acgaagtgtg
    57901 caggcgaaca cattgactgc ctcctgagag ggacgagcga accccgtctc cggcacggga
    57961 ttgagtggta tgaggttgac atggcacaaa gtgcccccca agaggcgcgc cagccgcagg
    58021 gcatggtccc tcgaatcatt aacaccgcct atcagagaat actcgtacgt cacgcgtctt
    58081 cccgtggaat tcgcataatc aatacatgcc tcgatcagct gccccactgg atagacctgg
    58141 tttatcttca tcaatgcaga ccgtgtgtga tcatccggcg catggagtga tacggaaagc
    58201 gtcacaggca gcccttcttg agcaagcctg cgtatgccag gcacgattcc gcatgtcgag
    58261 atggttatgt gcctgtagcc tatcccgtag cctctcttcc agttgataag tcgcacaaac
    58321 ttgagaacgt tatcgtaatt ggccaagggc tctccgcttc ccatcataac gaccgaactg
    58381 acgcgcctgt tgggactttc gttggcgatg ccattgctca cggccaatat ctggtctatc
    58441 atttcgccgc atgtcaggtt cctcacgagg ccctcaaggc ctgaagcaca aaaagcacac
    58501 cccataccgc agcctacctg agacgaaacg cacaagctca atccgtagtc atgctccatc
    58561 aagaccgtct caacagcatt gccatctgcc aggcctatca gatatttcgt ggcgcctccg
    58621 ggcgaggagt gcacggcaag gacctccgga ggacgcaagg gagcgacctc cgcaagcgtt
    58681 ctgcggagcg agacagggag atttgtcatc tcatcgtatg atgccgtccg cttgccatga
    58741 agccactcga aaacctggtc cgccctgtag gccggctccc cgagcgacgc aaaaaaccct
    58801 ctgcattcct caaatgacat gttctttata tccacagtct taatcattcc accaaatcac
    58861 aagaaatcct ctgtcggcgc actcccgccc tccgtaggct acccatgtgg caacctaggt
    58921 ggcgaacagc agcgcaggtt ccatcacatc cgctatcaat gaaggcttgt ctgttctcag
    58981 tgccattgca acgacacaaa ggcgacccta tcacgggagg ggtcgcctct caatgccatc
    59041 tgcgccatca cagccccatc tgaatggcgt cgacatgtcc acagcaatac tctattgcgc
    59101 aacttgaaac gcctatcacc aagatgcgtc caagccgtat cccgctccac cgccaagcaa
    59161 agtgatcagg cgcccccgtc acgcctgaca tagggtttcc cgcttgcagc tggcggaacc
    59221 gaccgcccga tgatgcccgc taatgccgcc atagtaacga tgtacggcgt catgatcaag
    59281 aactccctcg gaattgcagt aaatccccag ttctgtgctg tcagctgcaa tccgtctgcc
    59341 agaccaaaca ggagcgaagc ccacatggct cccacaggag tccatctgcc gaatatgacc
    59401 gccgccaaag ctatgaaccc cttgcccgcg ctcattccct ccctgaagag ggagagttgg
    59461 ccaagggata acgtgacgcc tcccatgcct gcaaggaatc cactgagtat cacgcacaag
    59521 tacctgatac cagcgacgtt cacgcctact gtgtccgcgg cctccgggtg ttcgccgcaa
    59581 gcccttatcc tgagccccag cggagttttg aaaagcacta tctgcgcgac tatgacgaca
    59641 atgaatgcga tatatacagg aggcatattc ctgtagaaga tatctccgag aatcggaaca
    59701 tctttcaaga agggaatggt ccatgccgca aggcccggaa caggttcagt ctgtccggct
    59761 ctgttgaata tggccctcag caggtagcct gttagagctg cccctagtat gttgatcgcc
    59821 acgcccgaca cggtctggtt tgcgccgtgc tttatgcata cccacgcatg caaagcggcc
    59881 accgcggctc cagacaaagc gcccatccaa aaccctatcc aggcattgcc ggtaagatgg
    59941 gagaagagaa cgcccatgaa agaaccgatc agcatcatgc cttccagccc tatgttgacc
    60001 acacccgacc tctctgaaaa cacaccacca atggccgtga gtatcagagg tgccgcgagg
    60061 gacaccgtag tagctaacgc gtcagccaca aaatatagga attccatatt attgcgtctc
    60121 ctccttcccg gaagcctcaa ggctggccgt ccgacgaacc gggagccaac cttcgacaat
    60181 tccttcagcg gccaccagga agactactga agcctggatg atgcctgcag tctcctttgg
    60241 aaccctggcc aacagctgca tcatctgtga gcctctgttg agcacgccaa agagcgaagc
    60301 cgccgctaaa atgccgtagg ggttgccctg cgcaagcaat gccacggcta ttccatcaaa
    60361 tccaaagccc tcaaacgcaa aaaggtcaag gaacttgtac tgaattccgc acacttgtat
    60421 agcacctgcc agcccagcca tggaacctgc aatcaacata gaccttgcca tagccgaaga
    60481 gacatcaatg cctgcatatc gcgccgcttc agcattgaga cccactgccc ttatctcata
    60541 cccccgcgtc gtcttgtaaa gcagacgata tatgagaatg gctgcagcga tcgcaatgag
    60601 aataccggag ttgaccctga attccgatcc atgcccgcca acgaatctcc atagcttagc
    60661 cgagtccgca atttcagggc tatacggcga cgccaaattc ggatccttta tcggaccatt
    60721 taccaagtag tgggataaat acagcgcgat gtagttcatc ataatgctgt tcactacctc
    60781 gtgtacaccg aacttggcct tgagataacc tggaactgca gcccagagcg ccccggccaa
    60841 cattccaaag aagatcgtga gtggaagatg gattatccgg ggcaaaccag ccagcttgta
    60901 gccgatgatt gccgaggcca acattcccac gatcatctgg ccttctgcgc cgatgttgaa
    60961 caatccggca cgaaatgcga ctgcaatgga caggccagtg catatgagag gcgtaatata
    61021 tacaaccgtc tccgaaaggt tcactgccga tccgaaacat ccgctgagca gtgcgccata
    61081 cgcatcgaga ggattcgcgc cagtgaataa catgaaaatc gccccaatta ggagtcccaa
    61141 gaccaccgca ataatgggga caatcagctg acgccaccac gccttccgtt tactctccac
    61201 gctcatctgc ctccgcctcc cgccgtctcg gccttggggc cgcgcatcgc tcctgccatc
    61261 atcaatccaa gctcttcttc ggttaccgta tcagaatcaa ctacccctac tatttcgcct
    61321 tcatacatca ctgcaattct gtcggccagg gccaggattt cgtcgagctc gagcgagacg
    61381 aggagaacgg cctttccgtc gtctcttgcc tctaccagct tcctatgcac gaattcgatc
    61441 gcgcccacat caagcccacg agtgggttga gcagctacta tgagctcagg ctgtcgcgaa
    61501 agctctctgg caactataac tttctgttgg tttcctcctg acaaagagcc cgccgccacc
    61561 ttttcgctcg gggttctcac gtcgaactcc tctatcagcc tgcgcgcctc ttgatgaatg
    61621 gtctcccagt caagttcccc acgtttgctg aatggaggtt tgtagtaagt ctgaaggatc
    61681 atgttttctg caatcgtgaa gtccagaaca agtccgtgtc gatgacggtc agacggtatg
    61741 tgaacgaccc cggactcaaa gacctggcgt gggcgagcgc cggtaagatc atgaccgtta
    61801 atgacaacgc gcccggatgt cggccgacgc atcccggtga tcgcctcaac gagctcgctc
    61861 tggccgtttc catctacgcc ggcaatcccg aagatttctc ctgcacgaac tgaaaacgag
    61921 actccacgaa ccgctggaag atgccgctga tcgagcacgt gcaactcgct cacttgcagt
    61981 atgtttttgc ccggctttgc gggccccttt tcaacgacga gttcaacatc gcggcccacc
    62041 atcatcctgg caagttcttg ttgagtcgtt tcggcggtcg caactgtgcc tatggacttg
    62101 ccccgtctga gcacagtcac atgatctgat atatccagca cctctttgag cttgtggctg
    62161 atgaaaatga tcgatttccc ctgttccgta aggttccgca tgatctcgaa aagctcagtt
    62221 acctcttggg gagtcaacac cgctgtaggt tcatcaagca ccagtatttc agcgccgcgc
    62281 agcagtgcct tgagtatttc caccctttgc tgcatgccca ctgaaatatc gacgacctta
    62341 gcgttggggt ccacagccaa gccataggct tcggatacct cgttggcctt tgctatggcc
    62401 ttctccatgt ccatgaaaag cccattcttt acgggctcag ctcctaacac aaggttttct
    62461 gcaacggtga aaggaggtat gagcatgaag tgttgatgca ccatgccaat gccaaggctg
    62521 atggcatcat taggctcctt gatggaaatc tctttcccat taacaagaac catcccctca
    62581 tcggcctcgt ataatccgta gaggatcttc atgagcgtcg tctttccggc gccattctct
    62641 ccaagcagcg cgtggatctc gcccttctcc aatttgaaat cgacattgtc gtttgcaacg
    62701 actccaggaa actgcttggt aataccgcgc atttcgagca ctgggacact ctggggcctt
    62761 cccataaaag tccctccagt aagatagcat tatgaagata gcacagttgg gcgccagacc
    62821 ctgaagcagc tggcgcccaa cctcagacta cattaggttc gcgctgtttc agcaatacct
    62881 tgattcacgg ttctctaatt gttcgaatca gataagaaaa ccgctaccga agatcgctgc
    62941 ctcgttctct tctacttctt cttgtaaagc ttatctgcct ccgcagggaa ctcgggaact
    63001 acgtactcgc ccctagcaat gatgtttgcc cattcgcgag ccttggccgt ggctgccgcg
    63061 gatgccctgt tagacttttc gggcgcaagc ccaactgcgt tttcgcgcaa gcccagaacg
    63121 accgtccgtc ccgtaaagcc cggcttcagc aacatcttgg tgccctcaaa aactccaacg
    63181 tccactctct ttatcattga gttcaagacg ttattcgggg ccagatggtt ctggtcggaa
    63241 tcgacaccta tggcccactt gccatgatcc tcggctgcgc ggataacccc gattccggtg
    63301 gaaccggcag cgtggaacac cacgtcggca tttctgcgga acatcgactc ggcaatggtc
    63361 ttgcccttgc caggatcgtc aaaggcgcca ctgtagttca ctatgatgtt tatgtttgga
    63421 ttggtcttcc atactccggc cttgtatcca gcttcaaatc tgtggattac cggaatatcc
    63481 atgccgccaa caaagcccac agtgttcgtc tttgtggtca gcgccgccag cactccgact
    63541 aggaaggagc cctcgtgctc tttgtacaat attccgagaa cgttggggat ctccttgtat
    63601 tctgcatcac tgtagccatg gtcaatgatt ccaaacttgg tattcgggaa ctgcttggct
    63661 accttctgaa cggcgtcagc catcaaaaag ccgatacccc aaacgacatc ataaccttct
    63721 ctggcaaggc tagcaaggtt aggctcatag tcagatggtg tgttcgattc gacatatcga
    63781 gtgccataac ccaactcttt cttgagacgc tcgaggccta cattggctga gtcattaaag
    63841 gactggtcac ctagaccgcc gatgtcaaat acaattccaa ctttgggcgc aggagctgca
    63901 aagccgttga atgcacaacc cacgagcatt cccgcagcca ggaaaacaac taggacacgt
    63961 ttgagagagc tcattcaata atcctcccct ttaaggtggt ttgaatctaa ggttaaactt
    64021 cgctatgtaa tggccaactc cttcccagta atgggctgag tttcatcgct caaccatgtc
    64081 aacgcggtca gcggttgatg cgaaatagcc tcatagtgtc ctctcttccc aactccaaac
    64141 aatcttgctc cgcgtcttgg cgcgtcaaac tccataccac gtaccttagc ttccgtacag
    64201 tcgtttgctt tcccgagtta ctcaactagc cacgtccata aggatgcgcc atccgaacta
    64261 acacaagctc tttctcgtca gcaactcgac ttgcgatgta tgcacactta catcgtacat
    64321 cgcccatgct caccaccgag gcagtcgcca tctaaaataa gattggtgcc cagtcctatc
    64381 ctgagtgtgt ggtcgatatg catcagtgaa taagtcgcca ctttatgcag caggagtggg
    64441 aaagaaaatg gagaattatt aaattataac cgcgatcata gtatatatgg aggtaataaa
    64501 atggcaggcg gccagtatga gcaggtacta gcgcggcttc gcgaggcagc tcgaatcctc
    64561 aatcttgagc ccggcatatt cgagatcctc gcagaaccag agcgcaccct cgaggttgcg
    64621 cttcccatcg aaatggacga cgggagcatc aaggtattta ctggatacag gtgccagcac
    64681 tccaccgtac gcggtcccgc aaaaggtgga gttcgttatg ccatggacgt atgcatggac
    64741 gaagtgaagg ctctctcttc gtggatgacc tggaaatgcg ctgtcgccgg aatcccttat
    64801 gggggcggca aaggcggcat tcgcgttgat cctacacaac tttcagagaa cgagctaaaa
    64861 cgattgacac gacgttatgt ctacgctatc taccccatta taggacctga aaaagacata
    64921 cccgctccgg acatgggaac aaacgcccag atcatgggct ggttcgtcga tacttattcc
    64981 gtccttaacg gagtctacag tccgggtgta gtcacaggca agccagtccc gatcggcggg
    65041 tcaattgggc gacgcgaagc cactggacgc ggtgtggtct tcgccgtccg cgagttgctg
    65101 cttcggctca ataagaagcc atctgagatc acatgtgcag tgcaaggcta cggaaatgtc
    65161 ggttcagttg ccgctacctt agcccacgaa atgggttgca aggtagtagc cgtgagcgac
    65221 attagcggcg gacactacaa cccgggagga ctcaacatac ctcacataac caagtatgtc
    65281 gaggagaata gagttcttga aggctacagc gaaccgggag tcagcaatat atcaaatgct
    65341 gaccttctca ccctcgacgt cgatcttctt atcccggccg cattgcagaa tcagatcact
    65401 gaggaaaacg cagaagacgt cagagcaaag tttatcgttg aaggcgcaaa cgggcccact
    65461 actgccgagg ccgacaagat cctcgcggca aacggcgtcc tcgttgtccc ggatatcctt
    65521 gcaaacgcag gcggagtcat ctgttcctac ttcgagtggg tgcagaatct ccagaacttc
    65581 tactggactg aggaagaagt caactcgagg ctagacaaga ttatgaccca tagcttcgca
    65641 gaagtctggg atttcgctca tgaagaagat ctcagtctcc gtacaggcgc ctatattctc
    65701 ggagtgaaaa gggtagcgga cgccatcaaa atgcggggca ttttcccgtg atcgcggccg
    65761 aaatgaccca aaactaacct gatcccgagg gacgccactg accggcgtcc ctttgccatg
    65821 atctaccgct gcactgtgcc gccacaacca tggcatgctg gccgcaaggc ctcgttcatt
    65881 tatgcccagc tacaagcttt ggcattctca acccgtagcc agcctgatgc caggcgctgc
    65941 gagcatgctc tacccatgtt gggtatgttt ctgcgaaatg ctccttcacc gtcaaatcac
    66001 agccggcccg atagcggcta cagatcctcc cgcctcaggc cggccatgtt tcaagcgtcg
    66061 tactacttgc atgctatatt gaccttcaac atcataccta acactgtatc gatagcgagc
    66121 accgtatttt gcgctacacc atgttcagtg ccgccttgat tgtcttgctg agctcgcaat
    66181 tgatcaccaa ttgccatctt tgccgcgcat cgagtttcag cggcgatgtg atgtgccact
    66241 attccaaaac aggctccgca caggcacaac agctcctcaa gaaatgcctg atccatgggc
    66301 atttgcgcct acttaatcag ttctcttcca gcttccaacg ctttgaggtt gagtggtata
    66361 aggtcatgcc tccttggagg cagtacctcc ttaagactct cagctatgga atcaattgat
    66421 accacgcgtg tcgcagcaag atacgcgcca agggcgcaca tgttgcccac ccgatcagat
    66481 ccaagctcgg cagccatgtc gttgactgga actgcaatca cccttacatc cgtgcgaatc
    66541 ggagctctat ctatcagaga actgttgtat acgagcagtc cccctggcct cagtgcggat
    66601 tcgaacctgt cgagtgaagg cctattcatg actatgaccg ataatggctc cgacacgata
    66661 ggcgaaccta tcggatctgt tgatatcaca accgagcagt tggcggtgcc accacgcatc
    66721 tcaggcccat atgaaggtat ccacgacacg tttttccctt cgagcaggcc tgcataggac
    66781 agcaactggc caataaccat tacgccctgc ccgccgaatc cagatatgat cagttcctca
    66841 agcattattc agccccccct tccggagttc ggaactctcc aaggggatag tatggtatca
    66901 tgttctcctc cagccattgc attgcctcgt atggcgccat tccccagttt gtcggacatg
    66961 tagacaagca ctccacaagg gcaaatccct cgccggccag ttgcgtctcg aatgccttcc
    67021 tgatagcagt ctttgccttt gcaacattcc ccggggatga aagtgcaacc ctggcgatgt
    67081 atgctgcacc gggaatcgtg gccaacatct ctgaaatccg aagtgggctg ccgcagtgct
    67141 ccttctgtct gccaaaaggc gcggtagtgg ctttctggcc caccaacgta gtcggagcca
    67201 tctggccgcc ggtcatgccg tatatggcgt tgttgacgaa tatcacagta atgcgctcgc
    67261 ctctcatcgc ggcatgaact atttcagcgg ctccaatcga agccaaatcg ccatcaccct
    67321 gatacgtgaa tacgaccgag tcgggcactg cccgctttat tcctgtagca acagccgggg
    67381 cacgcccatg tgccgcctgc tggaaatcgc agtcgaagta gtcgtatgcg aagactgcgc
    67441 accccaccgg cgccactcca atggcgcggt ccctgatctc gagatcgtcg atggcctccg
    67501 caaccagttt gtgcactatc ccatgggtgc aaccggggca gtaatgcgtc gaccttggtt
    67561 gcagtgactt tggaacagaa tatacttgct tcatttccgc acctccgccc tggcaatctc
    67621 ctcgacgcgc gccataatat cggcggcagt tggaatcacg ccgccggtag ttccgtaaaa
    67681 gtggactgga gcagcaccgt acaccgccac tttcacgtct tccaccatct gcccgaggct
    67741 catctccacc gtcaagaaag ccttggcagt gcgggcaacc tcggcaatgt catcgtacgg
    67801 gaacggagag accgtgatgg gacggatcag tccgaccttg agtccggcag cgcgagccct
    67861 gtccatgact gaccggcaca ccctagcagt ggtcccgtag gccactagca cgatgtcggc
    67921 atcatcagta ttcgccttct cgcacatcac ttcgttcttc ttcgcctgtt cgaacctttc
    67981 ggctatcttg aggttatgtt tgaacagctc atcgggtttc aggtagatag agtttatcag
    68041 acgtggggcg cgtccatcct ttgccccggt agtcgcccag tccttgggcg ggagttctct
    68101 tggcacagga tcccggaact ctactggttc catcatctga ccgagcaccc cgtctccaag
    68161 gatcatcacc ggcatacggt atgtttcggc cacatcgaat gcctgcatag tcaagtcaac
    68221 catttcctgt atcgatgcag gggcatatac gattgtgtaa taatcgccat gtccgccgcc
    68281 cttcactgcc tggaaatagt cgctctgagc cggctgaatt ccgcccagcc ccggtcctcc
    68341 ccggaccatg ttgactatga ggcacggcaa ctcggctccg gctatgtaag atattccttc
    68401 ctgcttgaga ctaatgccag ggctcgatga tgaagtcatt acgcgcgctc cggttccagc
    68461 ggccccataa accatgttta tggccgctac ttcactctca gcctgcagaa acaggccgcc
    68521 gacttcaggc aaccttcggg ccatgtactc agggatttcg ttctggggag ttatcggata
    68581 cccgaagaag tacctgcacc cagccctgac ggcagcctca gcaacggctt catttccttt
    68641 aaacaatttg cgttctacca tgcatactgc cctcctcagg gtttgaatac ttcgataaca
    68701 gcgtcaggac acattcgtgc gcaaatcccg caagaaatac agttctcctc atctgcgagg
    68761 acagcggggt gatagccttt ggtgtttatc ctctcggcca taattatcgc tttcttgggg
    68821 caaaactgca cacacaaccc acaacccttg cacttttcct cgtcaaatac aactttcgtc
    68881 atcggccgcc tcctcccaac atcattcgtt tgggttcctc aagaccccgg aggaccctga
    68941 ggcctgcatc ggccagcgcc tccatctctg cttcaccagg atacaccatc acgctggcaa
    69001 tgaatttgac tcgctcctca atccaggaca caagcatttt cgaattcgca gcgcctcctg
    69061 taaggatgat cgcatccaca tctcctgaaa gggcacaagc cacacggcct atctcctttg
    69121 ctacttgata ggccatacct tcatagcaaa tcctggcata atcatctcct tcctcaatct
    69181 tcttctcaac atccagcaac gaatttgttc caagatatgc cacaaggccg ccttgtccgg
    69241 ttatcttcct tctcatttcc gcctctgtgt atcttcctga gaagcagagc ctcaccaaag
    69301 acgtattggg aaggctaccg gaccgctcag atgaatatgg accttctcca tccaacgcgt
    69361 tgctgacctc gacaacgcgc ccgcgcttgt gtccgcctat gctgatgccc ccaccgaggt
    69421 gaaccactat gaagttacac tcctcatatt tccgccccaa atcgcgcgcg gctttgcgcg
    69481 ccatcgcctt ttgattgagt gcatggaaga cactccggcg ttcaatcccg ggaatgccag
    69541 tcatgcgggc aatgttgtca agctcgtcag ttgttgttgg gtcagcaata taagagggaa
    69601 tccccaaaga atcggctatt tccttggcta tgattccacc aaggcttgaa gcgtgattcc
    69661 atccccattc atacagggca tccaccagca catcatctac gatgtagact ccgccctcca
    69721 tgggaggcaa taccccacca cgtccaacca cggctgacag agtatctgca ggaaacggta
    69781 acctttccat gaagtcgaac accgcttcct ttcgcatatc aaactgatcg aagatgcggg
    69841 ggtaggaagc tatcatatcg gcatcatgtc tgagactttc ggacgccgct gaacgatctc
    69901 cttcaaagac ggctaccttc gttgaagtgg acccggggtt aatgacaaga accctgaaat
    69961 cgctcactcc aaccacctcc gttactatga cgttgcaata gccagcgcga gcgacttcag
    70021 cctggcttca ggagagtctg agcgcgacgt gagcacgatt ggagcacgag ctccggtcag
    70081 tatagccgcg gtctcaactt ggccgaagta aaccagggtt ttgtatatga cgtttcctgc
    70141 aacaatgttt ggaacaagca aaatgtctgc tctgccggcc acagggccat ccactccctt
    70201 gtggcgtgca gactcctcag agactgcatt gtcgaggcca agagggccat ctacaatgca
    70261 tccgcgtatc tggttcctat cgcacatctt ggccagcgct gccgcttcaa cagtctcagg
    70321 catgtctgga ttgaccacct cgacagcggc tatgcacgca accttgggca cttcgttgtc
    70381 gagcttgtgt gccaccccca ctgcgttctg aattatctgt actttgtctt gaagcgtggg
    70441 cgccactacc atagcaccat cggtcatgta aatcagccta tcgtacctcg gagatttgaa
    70501 gaccgccaca tggcttaata gcttcccggt ccggagacca acctccttat ctaaaacagc
    70561 cctaaggaaa tcggcagttg ggatcaggcc tttcatcaat atgcccgcct gcccggagct
    70621 gactagttca accgcgtgca gggcagcagt cctggcatca ggttcatgga caatttccaa
    70681 ttcagaaagg tcaaggccca ctgcggacgc cagcggtcgt atcaacttct catcccctac
    70741 gaggacgaac gcgcccatcc caatcccatg agccatttga atggcttcga gcacttcacg
    70801 atcttgtgca acagcaactg ccaccttggc tgaagggcgc ttcttcgcgc gcgctacaac
    70861 ctcatcgaaa gctgcaagca tcgacatctc ctccctcagg tatgtcttcg catcaaatcc
    70921 caatctgatc agactgatgc atatactctt gctttttcct ccccggcaag cactctcatg
    70981 acgctgctcg ctaaagcttc catctcgttc tcgccaggat aggtgattac gggcgctatg
    71041 aagcagcatc tctctgtgat gtgtccaacc agacgatccc aagctgcaag ccctccggtg
    71101 agaacaatcg cgtcaacacg cccacaaaga actgttgcca tggctcctat ttccttggat
    71161 atctggtagg ccatggcctg ctctacatct gacgcacgcg tatttcccgc aaggatcatc
    71221 ttctcgacct cgcgagcatc tgtagtccct agatgtgcaa aaagcccgct tccatgggtg
    71281 agtagatgaa tcaattcgcc ctcatcatac tgccccgaat aacacaaagt caccagatcg
    71341 ctcatgggca cagagcctga gcgctccggc gagaacgggc cttcatcatc cgccccattg
    71401 gcgtccacca ttttaccagc cttgaccggg gatatcgata tcccgctgcc aagatgggcg
    71461 accacaaggt ttgcctcttc cagcgccttg ccgagactcg cagcggcttt tgctgcagtg
    71521 cttcgtatgg aaagtgcatg cagtttggat acccgcgaga tctccggcaa tccggatagt
    71581 ctcgcaagag gctcatactc atctactgat attggatcgg caaaataagc ggggactccc
    71641 aatgagtcgg caaaggcacg ggcaatcgga gcggccagat tagcggcatg ctccgtggat
    71701 ggacggttga gaaggtcgtc taccacagct tcatctatca gatagactcc gccaggcacc
    71761 ggcttgaggg cgccaccgcg ggatatgatg gcgtctatct gctcgagcgc tacgccacta
    71821 ctagcgaggc tgcgctggat cacacctttg cgatattcga gttgctcagg gattctggca
    71881 tatcgcgcca gttcatcagg gggatgttgt atgttgtctg aaaagagcag tttgcagccg
    71941 cagaaaaccg caatcttggt ggatgtggaa cctgtgttta ccacgagaac tgtgaaagtc
    72001 cgatcaatgc atcccaacgg acccaccgcc ttcccaagtc gtacacagta ttgtatattc
    72061 tactttggta gccgagtccc ttcaaaaaac aaaaaagccg gagctattcc ggctatcttg
    72121 ctacaagcgc tgtatcctct tcacactatt cgcatcgaag tcatacgctc tttatctgcg
    72181 gtccctaccc ccgcttcttc cccgcggggc tccaccacca cgagttcccg atggcggtct
    72241 tctatctccg cgtgggggcg gagagtcagg catgccctca ggcctcggca gaagatcctt
    72301 gcgtgagagg tttatcctgc cttggctatc gatttccgtg actcgcacgt tgatatcgtc
    72361 gcccacttgc acgacatcct caacagtggc tactctctcg tgagcaagct gagagatgtg
    72421 cactagccct tccttgccgg gaagtatttc tacgaaggca ccaaaaggca tgatacgcgt
    72481 caccttgccg gtgtatacct cgcctggctc aggatcatgc accaactcca aaatcatgtt
    72541 tatggccttt tgggcctgtg cctcgtcgac tgccgacaca aaaacccttc catcgtcctc
    72601 tatatcgatc ttggtccccg taagatcgat gatcttccga atcatctttc cgccgggccc
    72661 gatcacatct cggatcttgt cgggatctat gtttatcgtc agcattcgtg gcgcatacgg
    72721 cgacaattcc gctcggggct gaggaatcgt ctcgagcatc ctgtccatga tgaaaagcct
    72781 tccgcggcgt gcctgctcaa gcgctgcctg gagtatttct ctggaaatgc cccgaacctt
    72841 gatatccatt tgaacggcgg tgattccgtc gcgcgtccct gccaccttga agtccatatc
    72901 cccaagggca tcctctatgc cctgtatgtc tgtgagtata gcgaagtcgt cgccgtattt
    72961 gacaagcccc atggctactc cagccactgg tttcttgatc ggcactcccg cgtccattag
    73021 tgaaagcgta cttccgcata ccgatgccat gctcgaagac ccgttagact ccacaacctc
    73081 cgaaacaact cttatagtgt acggaaattc ttcgtcgtca ggtatgacgg gcagcaaagc
    73141 ccgctctgca agagcgccat gccctatttc acgcctgcca gggccgcgca tcggctttac
    73201 ttcgcccaca ctgtatggcg ggaaattgta atggtgcata tacctcttag attcttctag
    73261 ccctagatcg tccaggatct gctcttcgcc tattgcaccc aaagtgcacg cggttatcac
    73321 ctgagtctgt ccacgcctaa acatcgcaga accatgaacc cgaggcaaaa gcccaacgtc
    73381 acacgtaatc ttgcgaatct catcaagagc tcttccgtcg ggacgggttt gctcctgcgt
    73441 gatcatcctg cgaactatct gtttgagcag aacatccaaa acaccgttga tgtcccgaac
    73501 gggaacctct tcaccgtata tttcggcgaa atgggccagc acttcttccc ttacagcatg
    73561 tgtcagctcc tcgcgagcta ccttgtcagg attgcgcact gcttgattga gcttgtcgta
    73621 cgcatactct tctaccttct ctgcgatctc cgggtctggc gtaaaagcca cgaactcctt
    73681 cttagaaacc ccaacctcag ctactatttt cttctggaat tggattatct ctttgatcgt
    73741 ttcgtggccg tatatgatcg catccagcac ctgctcttcg gggacttcat tggcccccgc
    73801 ctccaccatg agaacggcat cctcggttcc cgccactgtc agagagagtg ttgacctctc
    73861 tgactcgtca actccggggt tgataatgaa ctgcccatca acgagcccca ctttgacgcc
    73921 ggcaattggg ccgtcaaacg gtatgtcaga aatagagaga gcaattgacg cgccattcat
    73981 cgccaagagc gcaggatcaa tcccgggttc caccgaaaag actgtagcaa caacctgaac
    74041 gtcgttgcga aatccgtcgg ggaacaacgg cctgataggc ctgtcgatca gtcgcgccgc
    74101 aaggatagcc gcctctcccg gcctcccctc gcgcttgatg aacccgcctg ggatcttgcc
    74161 cacggcatag agtttctcct cgtaatccac gagaagcgga aagaagtcta tgccttctct
    74221 gggttccttg gacgctgttg cagtgacgag caccacagtg tcgccgtacc ggcacagaac
    74281 agatccatta gcctgctggg ctacgtagcc agtctcgact gccagtctct tcccagctat
    74341 gtccgcctgg aaaacttgat gcattaagtt gtttacctcc tacacattgc cttgttgcac
    74401 tcatgaacaa gagagcgggg cacaaatacc cgctctcatt cattctgcgc tacttgcgaa
    74461 gtccgagttc ttctatgagc gctctatacc tttcgatgtc gctatccttt aggtagtcta
    74521 gaagcgcccg acgtttacca accatcttaa gaaggccgcg cctagagtga tgatccttct
    74581 tgtggacttt caggtgttca gtaagatagt ttatcctttc tgtaaggatc gctatctgaa
    74641 cctctggaga acccgtatca gactcatgga gctgatactt ctcgataatg cttttctttt
    74701 cttcagttgt aaggctcaag ggctgttgca cctcctgttt ttcgtacgcc cttgccaaga
    74761 atgccgtcgg aggatcggca atcctagcca agggtcaagc aagcccgaat gatgcacgct
    74821 tgcggagttc attctagcac agatgatatt gggtgtaaac tccaattgcc tatgagagct
    74881 cgggtagcct cgacatccga cgcgatctgc gcctttaggt gagacgggct ggcaaaccgc
    74941 ttctcgtcac gaatcctagc cacaaattcc accattagca tttccccgta cagcgcctgc
    75001 gaaacatcga ccaaatgaac ctcgcacgtg gtccctcgac cctcgtctct gaatgtgggc
    75061 cgaacgccta tgttgcacac agccattgca cactggccat ctccccaaga tgctctggcc
    75121 gcataaactc catgagctgg aatagcatgc ccattcggaa gcctgaggtt ggcagtgggc
    75181 acacctatcg atttccccac accacggccg cgttcgacaa tccctctcag aaaataaggc
    75241 ctgccaagca gcctgctagc gagcactacg tctccctcgg cgagcgctgt tcgaacagcc
    75301 gtactcgcca ccgtaatgcc ttcgcaagtc acagggggaa ccacagttgt gccaattcca
    75361 tatctggcgc acatttcctc aagctcgctg gcaccggcgt tgccataacg accgaaagtg
    75421 tagttgaatc ccacaacgac atgccgagcg cggagtttgc gccacagaat atctgcaaca
    75481 aaggactccg gcgacatgtc tgccagttcc gcatcgaaag actggacaac cgcgacatct
    75541 atgcctgcgc tttcaataag cttcagcctg tcatctaatg atgtgagcag cggcggcgcc
    75601 ttcgaggggt caagcactgc aagaggatga acatcgaaag tgtatacgcc gcttcttggc
    75661 ccttcgcctt tgtcgcgcgc aagcgagatg gcctcgcgga tcagctccat atggcccaga
    75721 tggactccgt caaatgtccc taaggcaaac acctgagccg taacaggagg tctttctgat
    75781 gtggaaatga agaagcgctc catatgacac aacacctcag aaactacaac acaactcttg
    75841 gttggataag tgctcgtcca tccatcagac gaacctcgcc cacggcaaca agggccccat
    75901 cagcaccgag cagcgcgata tagtccccta ttccgcagac aggctctgcc cggttcggat
    75961 cccactctat ctgagtcccg tgcttgatac gcgaagcttc atcttctgaa accacgcatt
    76021 tcggcatgtg gcccacgcct tcaacgattg gcctgacaag attatcaaac tcgccatacg
    76081 cagctgcctg gcgaagctcg ttgagggtgc atgcttccga cagccgaaat ccggacgagg
    76141 ccgtacgttc aagcgccccc atgtgccctt ctatcccaat gctcgcacaa atgtcctcgc
    76201 acagcgttct tatgtaagtc cctttcgaac agaccacgcg catagagcct tgcgagccaa
    76261 gcctcacaac ttccggccaa tcttccaaag atgctggctc tatttcgtat atcgttactc
    76321 tccttggttc cctctcaaca gttatccctt cacgagcgag ctcataaagg cgccgcccac
    76381 ggtgacgcac tgccgaaacc atgggaggaa tctgctcaat atcgcctaca aagcgctgga
    76441 aagcctcgaa tactctttcc cgcggaacct tgacgtcctc acttacagca acgaccttac
    76501 ctgaagcgtc ccccgtgtca gtagatatgc cgaagacaat aaccgcctcg tattctttgg
    76561 gcgtctccat catgaacctt gaaagccggg tagcccttcc catacaaaca acgaggaccc
    76621 cggtggcatc agggtccaac gttccagtgt ggccgcatga gcgctcgccg gtgatccgcc
    76681 tcacttcaac aacaacatca tgtgatgtca tgcctttcgg cttgttgatg atgattattc
    76741 cgtccaagta tacccagcct ccgccaatgc ccggttcagc ctggaaatca cattcgattt
    76801 cacttctgca aggggacctg gaacagtgca ccctgaagcg cgcacatgcc ctccgccgcc
    76861 gtattccgca gcaatctcgg ccacgtccac cttctctttc gagcgaaatc cgattctgat
    76921 cagtccatcc tctacctctc gaaaagcaac agccacttct acgccctcaa ttgatcgagc
    76981 atagccgatg aatccttcgg tctcactctc cgtaatgcca tggcgttcca tcatctctgc
    77041 tgtgatctcc atggacgcga tcaggcctcc actcgagaac tgaatcccga ggattgcctc
    77101 ggccaaagcc gaaagggact tatagggttt ggtgtcatat atgttctgcg caatctccca
    77161 tggcctggcc cccgcgtcta caagggcagc agctacacga aacgtatccg cggaagtatt
    77221 cgaatacctg aatgaacccg tatcagtgga caaccctgcg aaaaggcagg ccgcaatatc
    77281 cgggtcgaac tccaccccaa gatcacggat aataccagct gccatctgag ctgtggcagc
    77341 tgcttccgaa tctacccagt tgatatcggc gaaatgagtg ttcgtggtgt ggtggtctac
    77401 attagcaacc ttcctgctgc ttactacggc gttttcacac tttcccaccc gatccaacga
    77461 gccgcagtcg agcactaaag ccaagtcgaa cggcccgccc acacattccg ccgtctctat
    77521 cgagtcagcc tccggcaaaa atctaagaaa ctcgggaacg ggatcagaaa ggccaacccg
    77581 cgggcctttt cccattctgt ccagcgctat cttgagtgcc agtgcagatc cgatagcatc
    77641 accatcagga gccacatggg tagtgatcag gatggacttt gcgtccacaa tggcctgcaa
    77701 cagttcttct ggagcgctca cttaggcatg tcaccgcttt cctcatcgtt ccgtccgctc
    77761 tggacctcct cgatcaattt gagtatgcga gctcctcttt caatggattc atcatactcg
    77821 aaactaattt cgggagttaa gcgcaacggc acgcgacgcc cgatctccga cctaatgtag
    77881 ccctttgcgc tttccagggc cttcatggtt tctgtgcggg attcttcttc gccaagtatg
    77941 ctgacgtata tcttcacatg ttgaaggtcc ccgcttatgt ccacatcagt cacagtcgca
    78001 aatcctagtc taggatcctt cagatcgcgc tgaagaatat tggaaacctc ttgacgtatt
    78061 gcggctttga cgcgatcagg ccgatagctc gacatcggac agcacctcca gagagtcctg
    78121 caagaactca gagtatttca atttcgtacg agtataacac gacatccgga gacagatcga
    78181 tcattctggc aactgcttcc aaaatgctgt gggcatgccc tgaatcgttg ctcacgcaga
    78241 cgatgcctat ctgcgcctta ttccatatat cctggcagtc tacttcagca acggctacat
    78301 tgaaccgatg tctgattcgg gccatcatcc cctggattac acgtttcttc tctttgagag
    78361 accggctgcc ttctatcaat agcacgcaac gcatggcgcc tatgaccacc gcataatcac
    78421 cccggcccct atagttcccg cttcacttct tccttcccgt aggcttcgat gatatcgcct
    78481 tcgcgaacat cattccagtt ttcgatgccg attccacatt caaagcccgc cgcaacctcg
    78541 cgaacatcat ccttgaagcg cctgagagac gcaatcttcc cttcatgaac aaccacgccg
    78601 tcgcgcacaa cgcgcaccga cgcactccgc ggcactctgc cctcagtaac atagcacccg
    78661 gctacaaccc cggccttggg caccttgaag gttgccctca cttctgcctg tccatacacg
    78721 acctcgcggt attcgggctc cagcaatccc tcgagggcct tcttcacgtc gtcaatggcg
    78781 tcgtatatga ccctgtaaag cctgacatcg accttctcgt cctctgcaca catggaagct
    78841 accggatcag gcctgacatt gaagccgatt acgactgcat tagatgcaga ggccaacatg
    78901 atgtcggact cgctgatagc tccgacgcct ccgtggatca catcgacacg cacctcatcg
    78961 gtggaaagct gctcgatcgc ctgccgaacc gcctcaacag atccctgaac atccgccttg
    79021 ataatcactc tgagttcttt gacttcccct tcttggatct gactgaacag ttcttcaaga
    79081 gataccttct tgggagcttc gccaatggtc tcaatgcgcc gccgttctgc ccgtctagcg
    79141 gcgacctgtt tagcctcgcg ctcatccaca acgacttgga acacgtcgcc cgccagcgga
    79201 acatcgccca agcccagaac ttcaactggc atggatggcg tggcctcctt cacaggctcg
    79261 cccttgtagt tcgtcatggc tctgactcta ccatgcactt ggccgacgag gaagtagtcg
    79321 cccaccctga gcgtcccctt ctggatcaac acagtagcga ccggtccacg tcccttgtca
    79381 accttggcct cgatgacgat tcccatggct ggcctgtcag gattagcctt cagttcactc
    79441 atgtccgcga tcagaaggat catctcgagg agatcctcaa tgccctgctt atgcaaggcc
    79501 gatacaggga ccatcactgt atccccgccc catttttccg gcacaagccc gcgttcggac
    79561 agctgcgtcg ccacccgctc aggattggca tcgggcttgt cgatcttgtt caccgcaacc
    79621 actatcggca ccttggcatc tttagcgtgg tttatcgcct cgacagtctg gggcattaca
    79681 ccgtcatctg cggctacgac cagcaccgca acgtcagtcg catgcgcgcc acgagcccgc
    79741 atggccgtaa aagcctcgtg cccaggcgta tccaagaagg ttatcttcct gccgtcaagt
    79801 tccacctggt atgcgcctat atgctgtgtt atcccgcctg cctccgttgc agtcacattg
    79861 gcctctcgta tggcgtcgag aagagaagtc ttgccatggt caacatggcc cattatggtt
    79921 accaccgggg gccttggttt caagctttcc ggaggatcct ctatctcagc tatgacaagt
    79981 tcgcgttcct ccctttcgga ggtaagctcc gcctcaaagc caagctctga cgcgagtatc
    80041 tgcgctactt cgccttcaat ctcgtcattg atccgaacca tcacgccaag cttcatgagt
    80101 ctgcgcagac aatcactggc cgatatctca agtttggcag cgagttcacg aacgctgata
    80161 gtctccccca cctcgatacg cctgaccttc ggcgccttct cgcgcttgcg gcctccccgc
    80221 ttcacggcga tctcgtctat gtcttcgata tcccctgtcc tcactcgatg ctcgagcacc
    80281 ctcgcatctt caagcggctt cgaagtaggc ggctttttgc ggccacgccg atctcccttg
    80341 cgctcggcac gtcgcggtcc tagttcatca tcaacgtaat cctcgcgccg accacgtcca
    80401 ccctcagatg ggcgttgcct acccttaccc tgttgtttag caggggaccc ttgtggagca
    80461 cgtggctgtt gctgagcgga tgcgccgagg ccacgttcac tgttctgacg cccgccagca
    80521 tgagacgcat ctccaggaag cctggaccgg tcgcgcacag ggccgctatt ccctccccga
    80581 tctctgcgat catcacgata tgggggggct gccccgccct cctgccgagc accctgaggc
    80641 ttgcccgcac cagctgggcg cgacgccgct tgaggatatg cccggccccc gccaggttga
    80701 cgttgaacct tctcctcttg cctagcgctc ccctgggcac tcgggggaga ttgctccggc
    80761 tttctggctg gctcagcctg ttgcgccgat tcctcagact tccgatcttt ctgctgggca
    80821 tcggctttgg cgcctggcgc gtcgggacca tacttgcgca tcacatatcg tgccgcgttc
    80881 tcatcgatag aactcatatg gttcttggcc ttgacgccca actcgttgag gagatcgaca
    80941 aggtctttgc tctgcatgtt caaatctcta gccagctcaa acactctcac ttttcccatt
    81001 ggcactcacc cccatcactc gcatcgggcc agattctcgc cgagcatacg ataaccccga
    81061 ggcgcacgca aggtccgtat cggctcctat catgaaattc cggccgcttg cgcgagaccg
    81121 cagaactcaa caacatttta caattcatgc cgcttatcat tgatcaaccc cacaagcaat
    81181 tcagtcagac agttcctcct ggagacgcgc gatgatttca tccggcatat ctgtttcaag
    81241 cgccctcgaa agcttgccgc ctcgagcggc tttggccaga caatcgcggt tcgggcacaa
    81301 atatgcgcca cgtcccgcgc gtttcccgct cgggtccaaa agcacttgac cttcgggcgt
    81361 acgcaccacc ctaacaagat cgcgcttatc ccgaacagcg ccgcaaccaa cgcacgtcct
    81421 ctgaggaacc tttctcgcct tggccataca accgcacccc taaaaatcta tctcgaattt
    81481 tggcatgctg accaaaccga cctgagactc gctcttaata tctatcttcc agcccgtaat
    81541 cttggcagcg aggcgcgcgt tctgtccttc cctaccgata gccaaagata gctggtaatc
    81601 ggggacaact acagtcgcaa ccttcgatat gaagtcgggc ctaacctcta gaaccttcgc
    81661 cgggctgaga gcgttagcaa tgaataccga ataatcctca ttccatggta tcacatctat
    81721 cttctccccg gcgagctccg aaactatggc ttgtatcctt gcccccctag ggcccacaca
    81781 ggcgcctaca gggtccacgt tcgggtcttg ggcagcaact gccaccttcg atcgtgcccc
    81841 tgcctcgcgc gctatcccct ttatctccac aactccctcg gcaatctccg gcacttccag
    81901 ctcaagaagt cgcctgagaa gacctggatg agttctcgac agcatcacaa gcggacctcg
    81961 acctgtcttc cgcacctcaa ctacgtaaca cttgagcctc atgcccggct cgtaagtttc
    82021 acccggaatc tgctcgtgcg gcggcaagct agcctccgtc ttcccaaggt ccactatgac
    82081 gttacgcgcg tccacacggc ccactgttcc ggtgaccaca tccccctcgc gttcagaata
    82141 ctcatcatag atgacgccgc gttccgcctc gcgtatgcgc tgaaccacca cttgcttcgc
    82201 ggtctgagca gcgattctcc caaactcctt cggggtaact tcgtcctcca ctacgtcgcc
    82261 cacctggtag tttggatcca actgcctcgc ttcttccagc gagatttctg tctttggatc
    82321 atccgcctct tcgaccacgt ttttccgcgc aagcacgaga atgtgtcctg tttccgggtc
    82381 tatattcacc ctcacatttt gagacgagcc ataatgcctc ttaaacgcgg acaccagagc
    82441 agcttccaaa gcctccagaa ggactgcttt gggaactccc cgctcccgct caagttcatc
    82501 gagagcctgg actatctcca agccttcacg ctcagtcatc accgaaaacc tcctcgaggc
    82561 ggacagccag ccttgctgtg ttgatgctgg aacgcggcac ttgtacaaca acgccgtcca
    82621 cctcaatcaa gacagaatcg ccacggttgc cccgcagcac accttcaaag cgcttacgcc
    82681 cgtctatttc cttatacgtc gttataagcg cccatcttcc ctcaaacctg tcgtattcct
    82741 catctctccg tatcggcttc tcgcctgagg acgatacctc caatatgtag ctttgcggga
    82801 tgaaatcctc acggtccaac tccgatccga ccagtcgaga cagatcggcg caatcgtcta
    82861 tcccaactcc ccctggcttt tctatataaa cacgcaaata ccaagtaccg gaatccttca
    82921 caaattcggt gtcgactacc tcgagcccta aggagtcagc agctgactga gatatagcgt
    82981 tcgcacggga ttcgatttgc cgacgatcca tagttggcct tccctcctaa tgagtaaaag
    83041 cgcagggctt ttgccctgca aggggacaga catgatgaaa gagtgggacg gacccactct
    83101 ccctgaccac acatcacaca tgcctgctga ggacaatcct cacacatacc aatatatcac
    83161 acgccaaaga tccatgcaag atcgcccaac ccgccaaccg cgcggctagc cccgctcaga
    83221 aaagggacaa ctgatccgac tcgggaaggc cagcagtagc ccctatttct tccagcgtat
    83281 ctatgtgcgt cctggtcaac ttcgtccgcg accaaaggtc gtgaaccgag gtgaacacgc
    83341 gttccctcct cgcctcaata acgctggaag ccgcagtatc gccaagcgta ggcacggtaa
    83401 tgaggggagg acgaagccct tcctcttcta tgacgaatcg agcagcatcg gaatgacata
    83461 cgtgcaccgg caaaaagcgg atgcctcgca tcattccttc ctgcgccgcc tcgagtacgg
    83521 tagccagatt cctctccctc gatgtagcat cgttcttctc ctctattgcg tttcttgtgg
    83581 cgataatatc ttccatgctc atgcccacaa tggacgcgtc gaaatcggaa gcccttatgc
    83641 tgaaatacgc tgcgtagaat tccctgggcc agtgcacttt aaaccatgct atcctaaacg
    83701 ccatcatcac atacgccact gcatgggctt tgggaaacat gtactttatt ttattgcagg
    83761 aatcgatata ccattccggc accccgtgtt cacgcatgag ctccgggtca ccatcggcta
    83821 gcatcttgcc ctttcttacc cgctccatga tcctaaaggc gtctcgaggc gcaatcccat
    83881 cacttatgag tctgttcatt atgtcgtcgc ggcacgcgat aacttctgaa agccgggcct
    83941 ggccagactc gatcaactcc tgcgcgttgt tgagccacac atctgtccca tgtgaaagtc
    84001 cacttatcct cacgagctct ccgaaggtcg taggcctcgt ctgctccagc atccctctga
    84061 caaatttcgt tccgaattcc ggaatgccaa cggttccaat tacgaacccg cttgtctcgg
    84121 gatcaagccc caaagactcg acaccggaaa aaatcttcat agtgtcagga tcgtccatgg
    84181 gaattgtcga agcatcaacc ccggtcatgt cctccagcat cttaatcatg gtcgggtcat
    84241 catggcccag caaatcgagc tttagaaggc actcgtgtat gttcttgtaa tcaaaatgcg
    84301 tagtgatagt gtcggagtct ctatcgttag caggacgttg cacaggcgta aactcgtgca
    84361 catcccggct gccggggact atcatgattc cgcccggatg ctgcccagtg gtgcgtttca
    84421 cgcctgtgca gcccagggcg agccgatcca tttccacact gcgtttgact attcccctcg
    84481 actcgcagta cgccttcaca aatccgtatg ccgttttttc agccacggtt gcgatcgtgc
    84541 cggcccgaaa cacatgatca gctccgaata tctcctcggc gtacttgtga accgtagact
    84601 gatactcgct tgagaagttg aggtcgatat cgggcacctt gtcgccttcg aacccgagga
    84661 acgtttcgaa gggaatattc tgtccttcat tccgcatcga tgcgccacag cgtggacagt
    84721 tggcagacgg cagatcatac cccgactcca catcatgccc gtgatcgaag acgctcagct
    84781 tgcattcgga acacagataa tgcggtggca acggattaac ctcggttatc ccgcacatgg
    84841 atgccacgaa agaagatccc accgatcccc tagacccgac tggatacccg tctgacaccg
    84901 acttgtgcac tagcttggcc gcgatatcgt agtttacggc gtatccattt ccgcagatcg
    84961 cgtccagttc cctttcaagg cgcacattga cgatttccgg gagcggctcg ccgtaaagct
    85021 cgcgagccct tgcgcgggca cgcgtcctta cctcttcatc tgccccctct atctcggggg
    85081 tagcaagttc gtcgggaaca gggcgaactg cctccacctc gtcagcaatc tggcgcgtgt
    85141 tagctacaac cacctcataa ctggcatcgg gccccagaaa tgagaattct cgaagcattt
    85201 cctcggttgt gcggaaatac aagggcgcct gatggtccgc atcctcgtag ccctggcccg
    85261 ccatgagaat cctgcgatag acctcgtctt gtggatcgag gaagtgaacg tcgccggtgg
    85321 ccacaacagg gattccaagc tccttgccga gcctgtatat gcgaaccgat atctcctcca
    85381 cctcttcgat gcttgcgagc cgcccggatc tgaccagcca agcgttgttt cccgaaggca
    85441 tgatctcaag gtagtcatag aaggacgcga ccttggccac ttcatcatca tccgcgcccg
    85501 agagaatggt cctaaagagc tctcccgctc cgcacgccga ccctattagc atgttcgacc
    85561 ttgcctgctc gagttcgctc ctgggaatcc tgggatggcg ataaaaatgt tccaggtggg
    85621 agagggatat caaccggtaa agatcacgga gaccttcgag gttcttagca agcaacgtga
    85681 catggtacgt atgtccgttt tcatcagagg ggtcatcgac aagatagccc tctatcccgt
    85741 atatcacctt tatgccatgc tttcgcgcca cattgcaggc atcgggaaaa gcttgtgcta
    85801 caccgtggtc tgttatggcg atagcaggat ggccccacga cgccgcgcgc accacggcat
    85861 cttcaacatt gcacaccgaa tccatcgaac tcatcttcgt atgcaaatgc aactccacac
    85921 gcttttcaga ggccaggtcg atcttttcct ctatctgatg gggaacaatg tcagacgcca
    85981 ttagaactat ctcacgagca tagtggtcgt attgaacctc tcctctgaca cgaacccatt
    86041 ggccgctctc cagatgaaac ctggactcat ccccgcggaa gaaacattta cacattatag
    86101 agtcggattt gtcggtaatg gcaaattccg taatgatgaa gccgtttcgc gtatgcctct
    86161 cctcgaattt gaagacttgt cccgcaacga caaccttctt ctgcccttca tgcagctcgg
    86221 aaatggactg cggagaatct gagatcattc tccccagtat ggcgccttct gcgggcttcc
    86281 ccctcgagcg tcgccgacca ggcgacctcg aactactccc ttgccgttcc gccttagcag
    86341 agacaacgct ttccagcggg gccatcggct catcgagctc gacaggaacg gcgggctctt
    86401 caaactcgcc gacttcgatt ctgacctttg ggcaatggtc acatagttcc gaaaacgctt
    86461 gagcaatatc gcggtcgcca gctttctcct tcagcttggc aacttgtaca acccctggaa
    86521 cttcgacaat gacacctacc gaatcggccc ggcatcgagc ctgagccagc caaacgccga
    86581 cgctcggaga gttcttgcac acccgttcga ccacatcagg ccagtacttc tctattatct
    86641 cgtcacttgg agtccaatcg tcagggccac ctgacgcggg ctcaacagag acagtgattg
    86701 catttcctag aacttttcga aaagcggcac atatctgatc gatgcactca gctccaggaa
    86761 aaacagcgcc ggccaggcgc actgtccaat gctttgactc cgaatctata cagatagtat
    86821 ctattgagca ggagtcatac aaggccagcg gaacactcat gccggcagat ttgaatatat
    86881 gtttgagttg agccttttca ctagatggac gaaggacaaa taccgtagac aacaactacc
    86941 ctccagcaat ccggcgggcc ctaatcccaa tcaacgattg atttgaggac ctcccagtac
    87001 attcgggcac gtgaggccat accctttaca gtgcccatct tctcttcttt catcacatga
    87061 gtcagtctcg gaagcttcac gtcaaccacc ttgagcccgg attccttggc atgtcgcgtc
    87121 agcacggcct caactccgaa tctggaaatg tcgagctcca aaccttccaa cacgctgcgc
    87181 ttgatagccc tctgccccga cagccaaggc gtcaaggcct gcgcaaggtc ggtggctgtg
    87241 cgtccattgc caaaaatccc gatcgtcata tccgcttccc cactgcaaac cggttcgatc
    87301 agggcgttca catgatcaac ggtcaaccca atgaggtcag catctagaaa gagaatcaca
    87361 tctcccagtg tagcctcgag tccccgcgct acagccccac ctttgcccgt attctcccca
    87421 agccttatga cattcgctcc atgggctctg gctacttcag cagtatcatc atcagagcca
    87481 tcatctacaa ctatgatctc gtccaccaga gggctcgcac gcacgacatc aagcactgac
    87541 gcaattctag gtccctcgtt gtacgcggga atgattgcgg ctacacgcat gcttgtcacc
    87601 tcctactcgg gcatgagcgc tgcaagttca cgatccacga tctctttgac acgcactgca
    87661 acattctcga tctttacaag ctcaacggtg ccgtcacgcc tcatgctcac ctcaacttcg
    87721 ccgtctccga ggtgtttggg ccctactatc acgcgaatag gaaatccgat caaatcggcg
    87781 tccttaaact tcacccccgg gcgttcgtct cgatcgtcca taacggcttc cactccatgc
    87841 ttgcacagtt cggaatatat ccgctctgcg gcttccactt gcgcgctttc cgaagtattc
    87901 agcggcataa ccacggcctg atatggagcc accgacatcg gccatattat gccatcgtca
    87961 tcatgatgct cctcaatgac ggaggcaacc gtgcgcgtga ttcctattcc atagcatccc
    88021 attattatcg gatgctcgac tccaccctca tcaaggtatt ttgcccccaa agcagaacta
    88081 tatttcgtcc caagcttaaa caggtggcca acctctacgc cacgcgcccc gcagagctcc
    88141 gctccacatc taggacaggc atcgccggcg accacatttc ttatatctgc aacgatatct
    88201 gctgtatagt ctacaccata ctctgcacct accaggtgcg catcgacagc attggcccca
    88261 accacggcat cctcaacaac agttgcctcc caatcggcaa cgatcttgat tcgctcccta
    88321 agcccaaccg gccctgtgaa cccaacaggc gcaccgctta cgcgctcaat cacttcaggc
    88381 gtggcaagct ccagctcacg acagcccaaa acccttttga gtttggcctc gttaagatcg
    88441 cggtccccgc gcactatggc agctacgggt gtaccgtctg ccacgtagac aagagttttc
    88501 agcagcaccc gaggctccac cttgagatag cctgtaacct cctcgatagt ccgcataccc
    88561 ggtgtcgata tcttccttat tccagtacga actttctcgg tgtcagcaac cctctctgcc
    88621 ggcttagccg attcagcgcg ctcaacgtta gccgcatatc cacacgcgtc gcagaaaacc
    88681 agggcatctt cgccaacctc cgacacaacc ataaactcat ggttctggct tccgccaatt
    88741 gctccggcgt cggcttcaac caccctatag tccacaccgc atctggaaaa caccctgcaa
    88801 taggcctcat acatcttctc gtaactgcgg tcaaggccct cattatctct gtcaaagctg
    88861 tatgcgtcct tcattataaa ctcgcgcgaa cgcattaccc caaaacgcgg cctgatctcg
    88921 tcccgatatt tcgtctggat ctgatagagg atcttcggca actgtcggta cgaccttatt
    88981 tcatccctca ccgttaccgt gattatctct tcatgcgtag gtccaaggca catttccctt
    89041 ccgtttcgat ccttaagacg gaacatctcg ctgccgtaat catcccatct gcccgtttcg
    89101 cgccatatgt cggcggactg aatggctggc atgagaacct cctgcgctcc ctgcctatcc
    89161 atctcctcac ggactatctt ttctatcttg cggaccacac gacaaccgag atgaaggtaa
    89221 gaataaaccc cagagacagt ctttcttacc atacccgccc ttagcatcag ctgatggctc
    89281 ggaatatccg cctcagacgg cacttcccgc aacgtaggcg caaacatctg cgacatcctc
    89341 atcgtaaacc aactcctcta gcagttaacc gtgtgacttc tcgtgccggt tcgccgccat
    89401 cgattcggcg gcctccacga gcgcccctac catctctgcc tcccggactt tgcgaaccac
    89461 cctcccgccc gagaagatca ggcctacccc gtttccacat gcaagcccaa catctgcttc
    89521 gctcgcctct ccgggcccgt tcacctcgca tcccattata gcaactacga gtggtgcgtc
    89581 caggtgcgac aagccatcct tgacttcctt cacaatgccc gcaagatcag ttctacacct
    89641 tccgcatgtc ggacacgaca ctatcgttgg tccgaatcgc ctcaggccaa ggctggcaag
    89701 gatctcacgg cccgcgatta cttcatcccg gggatcccca gtgagactca ccctgatcgt
    89761 atcccccaat ccttcagaaa gaaccatccc aataccgata gccgacttaa tgagaccgcc
    89821 ggggggcggg cctgcttcag tgattcccac atgaagagga tacggcgccc tacgcgcgat
    89881 caatcggtat gcttccgtcg tcctcttaac gtccgacgcc ttgagtgaaa ggacaatcga
    89941 ttcaaatcct tccctttcaa ggagactgac atggcggaga gcggcctcca ccatggcctc
    90001 gggagtgcgg ccatactttg acaccaagtc ggctgggagc gaacctgcat tcactcctat
    90061 gcgaatgggg atacccctgt ctgcggccgc atgagcgacc tctcgaaccc tgtctgcacc
    90121 cccgatgttg ccggggttga tgcgcaattt atcgacgccc gcctctatgc tggccaaagc
    90181 caacttgtag ttgaagtgta tgtcggccac caacgggacg ctagatgctt tgcgtatgct
    90241 ccgcaaggag tcggctgctt cctcatctgg gacagcaacc ctaacaatct cgcatcccac
    90301 agcggcaagc tgccggatct gcttgatggc agcatcagca tcacgcgtat cagctgtagt
    90361 cattgactgg acagaaatag gggcgccacc ccctatcgga acagatcctg ccattacctg
    90421 tcttgtctct tcgcgagtcg ccatctgaca aacaccgcct gatccggcag tcagccgcat
    90481 agtctacatg cgttgaacca taatcctcga gatatcgctc acagtagcat acacgagcaa
    90541 gagcatgagc aacgctagcc cgataaacct gagaaaaccc tctacttgag gagagagcgg
    90601 cttccccctc acagcttcga tgatcagaaa cagaacccat ccgccatcga gaaaaggaat
    90661 tggcatcaga ttgagcagcc ccaggcttgt agagatgagg gccacaagca tcatcaagct
    90721 cggaaaaccg gcccgggccg cctgcgccga catgccagct atccctacag ggcccgccac
    90781 ctcgggcttg acccttcccg caagcgccgc tcccatcgaa gccaaggtag tgtcgactat
    90841 cccccatgta tacctcaacg aaccaactat ggcagcgccc gtcccagtct tgctatagac
    90901 ctctacgagg ctcataccta cccgacctat attctcctct gcatcgcgga taggggtcac
    90961 atcgatgatg atcgtctccc catcgcgcaa tgcctccact atgagtttct ttcccgcgct
    91021 cgaagccacg gtgtttataa acccagccac actcgctacg ggctttccgt ctatgcttac
    91081 tatcttgtcg cccgactcaa accctgctgc ctccgccgga cttccatcga agacttcgac
    91141 aactgatgcc atggggacac cgtatgacga gaagagaacc gagagtatga ccacggcaag
    91201 gacgaagttc ataagtggcc ccgcgacaac tatgagtatt ctctgccata tagggcggtt
    91261 gaggaagctc ctgggatcat cctcctccat ctgctcctcc gggaggtccg aatcgtccat
    91321 acctgcaagc ctgcacatgc cccctaatgg gagggctcgc acggcataga gcgtctcccc
    91381 tctcttccaa gaaaacagct ggggtccgaa accaatggca aattcgtaaa ccttcacccc
    91441 acaagctctc gccgcggcga aatgaccata ctcgtggaca agcacgacaa ttccaagcac
    91501 caccaagaag gctagcgcgt acatgagcga caccttcctc tcacaaactc cctggcccat
    91561 acatccgctt caactatgtc atccaaagaa ggttcttcta ttcggggaga tgattccatc
    91621 gcttccatca cgaggtccac aatgtcgacg aaacgcacat caccgtggag aaataggcgc
    91681 acagcctcct catttgcggc cgacatgact gcaggaacca cgcctttatg cctcaaagct
    91741 tcatgtccca atcccgcccc aggaaacctg gaaagatcag gtttctcaaa ggtcagtttc
    91801 ccgatggagc tcaagtcaag cttggcagcc agcccttcga ccctttcggg gcatgtcagg
    91861 gcatactgta tcggagtgcg catgtctgcc gtccccagct gcgcgattat cgagccatcg
    91921 cagtactcca ccattgaatg gatgacgctt tcgcggtgga ccacgatatc tatcatctcc
    91981 gaatccacat cgaaaagcca gcgagcttct acgacctcca acgccttgtt gatcatcgtg
    92041 gccgaatcca cggaaatctt gggacccata ttccagttag gatgggatat ggcttccgcc
    92101 acagagatat cagcaaaatc atacctgtca atgaatgggc cgcctgacgc tgtaagaatg
    92161 atgcgtgcga cagcttccct gccctgtccc tggatgcatt ggtatatggc tgaatgctcg
    92221 ctgtctatcg gcctaatggc cactccctcc tgccgagccc gatccattat cacagaacct
    92281 gcagccacaa gtgtctcctt gttggcgagc gccacgtcgt gcccagcatc gatcgcagcc
    92341 aaggtaggcc atacccccgc aaaccccacg atggcattga ccactaaatc gtgttccgct
    92401 gccgccgcga cctgtgaaac gccttctggc ccaaaaaaca attccggcgg cgtactcatg
    92461 cgggagaata aagctctcgc ccgcgcttcg gcgcgcacat cgccaattcc aaccatggcg
    92521 ggaccgaact ggcgcgcctg ctcagctagg agctcgactt cggaataagc tgataagcca
    92581 ctcaccttca gccctagaga acgcgcaacc tcaagagatt gaacacccac cgatccggtg
    92641 gagccaagaa tgcaaataga tgtcatatct tactcctcat caaatggaaa cattggcagg
    92701 attaccgact gatatactgc tttgataaat atcactgcca gcggccctgc tataaagccc
    92761 tttgtgccaa acaggcgaat tcccacgtat atagacacga gagcgacgag cggatgaagg
    92821 tcgagattct cccccatcac ttttccctca gcaatctggc gcacaataga gttgaccgcc
    92881 aacactataa tcagacccaa cccaaaaccg aagtggccaa ctacgaaatg atacccggcc
    92941 catggcagaa tgagaagaga cgggcctacg accggcatca ggtcgaggag tgcacagacc
    93001 aaccccaaaa gccacgcata gctgatgccc atgatagaaa acccgataac cgtcattatg
    93061 ccagtcatgc acatcaatac aaatcttgat ctcagataac ccacaaaccc ggcaagcagt
    93121 tcgcctctga cctttcgcgc ttgctttcgc cacgatctcg gcaacgcctt cagaagattc
    93181 tcggaaaaac gcgggtgatc gcgggatatg aaaaacgtag agataacgct gagcatgaag
    93241 accagcgtga attcgggaag gccccccaga aaagatgcaa ctccagagac ccccgttcga
    93301 accacctgaa acagcttatc ctgattttcc cttagggctt ctacaaaagg aactggcaag
    93361 taggcgaaga aatcctcggt ccttcccaca aggttgtcca aggcctcgct gaacgaccgg
    93421 ctaaggccac ccatattccg cgatagctgg tcaatttcct ttgctatttc ggtaactccc
    93481 aaggcaatga gcagtccaag cacgagcccc aacactacaa gcactattgc aacggcaact
    93541 cccctggaga tcttggggtt cttttgtaca agcttgactc caggctctat catgatggcc
    93601 aagaaaagcg cgatcacgaa aggcctgaaa aggggaagca catacatgat caccagatat
    93661 gttcctacta ttagcccccc aagggatatc gcggccttgg cgttgttgct caccgagaca
    93721 cctccgcgca tgtgcgtctt cgacgaatta aagcatcaat ctcaggtaat agtatgcgat
    93781 cgggctgacg aagaagaagc tgtcaaatct gtccagcacc ccgccatggc ccgggaacat
    93841 cctccccgca tccttcacac ctgcatcgcg tttcatgcca gactcagcaa gatccccgat
    93901 ctggccggac gccgccacaa gaagcgcgaa aatgatagcc tgtggccaac tgagagtata
    93961 ccataagcca gtcacgtctc ccaaagcctt gtatgcgatt gacgcaagca cagcccagac
    94021 gaaaccacct atggagcctt caatagtctt gtttggcgac atgcttggag ccagcttacg
    94081 ccgtccgatc gctcttccaa caaagaatgc gcccatatca ttaaaccaca caagcaccat
    94141 catcagaagg gaaaggccta gcccagcatc gccaaaagtg ccccttatca gacagaagcg
    94201 gcttaaggtc catccacagt acacaacccc gaacacttca agggccggaa cggccatgga
    94261 acccgtaggc ttcgccttca tcacctgccc ggcaagggaa agagccaaaa ccaaggagag
    94321 caacgagccg ctgaacctgc caccggttaa aacggcatcg acaacaaaaa gggcagatcc
    94381 cgcagatatc agcggctgac ttggagtcca tgcctttgtc ttgcacatcc tggcgtattc
    94441 cataccgcct acgaccgcca aaacagcccc tacaatggct aagggcaccc cgcccaacca
    94501 caacacgaat atgccaatgg gcgcgcctat cagaccaacc aacaccctcg tcctgatcat
    94561 ccgccttcac ctcgaccgtt gtgaggcgcg gtctttagcc cgccatagcg tctcgtcctt
    94621 gacagaaaaa atgagatggc ggcatcgaga tcctcatcgc gaaaatccgg ccatagcgtc
    94681 tgggtcacgt atatttcagc atacgcgacc tgccacagca tgaagttgct gatcctgctc
    94741 tcaccgccag tgcggattat caagtccgga tctggctgac cagcggtggc aagccttgac
    94801 gaaatcagat cctcgtcaat gtccgacgcg tccagcctcc ctgcagcaac atcttccgcg
    94861 attcgacgaa gagccaaagc cacatcccat ctgcctccgt agttgagggc aatgttaaca
    94921 ataatcctgg aggcattcgc cgtgatcctc tcagcattag cggctactgt gcgaatcgac
    94981 ctcggaagtc ggctcctgtc gccgatgaca ttcaccttag cgccactctc agccagttca
    95041 tcagcgtttt ccctgaagaa ctcctccatc agcagaaaca agccctggac ttcagaaata
    95101 ggcctcttcc aattttcagt ggaaaatgca aacaaggtaa cgtaccctat tcccctgtcg
    95161 cgtgcggcaa gaacgatgtc cttagctcta ttcgccccga cttggtggcc atataggcgc
    95221 ggctttccct gttgctgagc ccacctgcca tttccgtcca tgattatccc tatatgcttt
    95281 ggaagcaaag gggttcctcc cttcggggcg attcaaggtg acatcgccca tcgcggcatc
    95341 acgccgagag aagattgaga tcatactata aaccccctcg aatcgagggg gttgtaagcc
    95401 ttagcaatgc acaaggctca gacctgcatt atctcgactt ccttatcggc caacatatta
    95461 tcgatgtctg caatatactt gtcagtcagt ttttgaattt cgtcctgagc tcgccgcagt
    95521 tcatcttcgg tgattatgcc gtctttctcg agaacacgaa actcctctat ggcgtcacgc
    95581 cttatccccc tgatgctgat tcgcttctcc tcagccatct tcctaacaag cttgaccaat
    95641 tcacgtctgc gttcctctgt aagatgcgga atagccatgc ggataacatt gccatcattc
    95701 gtaggaacca gaccgatatc agatttttga atcgctttct cgatcttacc caaaaccgat
    95761 ttatcccatg gcgttaccac gagcaaacgc ggttcaggcg ccgaaacgct agctatctgt
    95821 gtcagaggtg tcggagtgtc ataatagtct accactatcc tatccaggag ggctggattg
    95881 gcacgcccgg tgcggactcc agcaaaatct cgacttgtgg attcgaccac ttccttcatc
    95941 cgttcctctg ccagttctag ctcgtccttg tacacaccta gtccccccca atccacgtcc
    96001 caaccggctc acccatgagc gcccttttta tgttcccttc ggtgagcaag ttgaaaacaa
    96061 caatcggtat cccattgtcc atgcaaagcg aagctgccgt ggaatccatc acgccaagct
    96121 cgcgactgat gacatcaatg aatgataatt tctcgtaaaa ctttgcttct ggattcttca
    96181 gcggatcacg atcgtatact ccatcgaccc ttttcgccat caagatcaca tccgcctcta
    96241 tctcagccgc ccgcaaagcc gctgcggtgt cagtcgagaa aaacggattt ccagtacccg
    96301 ctccgaagat tacgatccgg cctttctcca gatgacgtat ggctctgcgc cgtatgtatg
    96361 gttcagccac ctcgcgtatc tctatggctg tcagcaccct cgtcggtatt cctgaatcct
    96421 caagggcttc ctgcagcact acggaattta tcactgtagc gagcatgccc gcatagtctg
    96481 cactgcttcg atccatgccc atccgacttg cctcagatcc ccgccagatg ttacctccgc
    96541 caacaactat agccaattgc gtacccaagg catacgcttc cgaaacttgg ccggctatcc
    96601 atcttacagt gtcgatatca acaccaaatc cccgttctcc tgcaagcgcc tccccggaca
    96661 gcttcaggac tacccggctg tatttgggcc tttccaagat tgtcactccc cgttggattc
    96721 tatgcgaacg ccaattttcc ttctaaatcc gtttcacaca ccacggcggc tatgcgaagg
    96781 agagaacacc ggacgtgttc tctcctcacc tgattgacct tatcaaagcg cctctccgcg
    96841 ttcgaatcgg gcaaatcttc tcactgtgat gttttcaccc aaagtcccta tcgtctcgcg
    96901 aacgaggtca ctcacggatt tttccggttc acggatgaag ggttgatcga gaaggcagac
    96961 ttcttgatag aacttctcca gcttgccctc tacgattctg tcagcaacct tctccggctt
    97021 gccctcgtta agcgcctgag ttcggtatat ttgacgttca cgttcgatca catcttccgg
    97081 cacttcgtcg cgtgatacgt acagcggcct tgaggcagcc acttgcatgg ccaaatcccg
    97141 cgccagggtg cgaaactgat cggtcttagc aacgaaatcc gtctcacaat tgacctcaat
    97201 cagcacgcct atcctgccac ccatgtggat gtaggactca actacaccct ccgcagtctc
    97261 cctcccggca cgctttgacg ccgcagcaag gcccttctcg cgaaggagtg ttacagcatt
    97321 gtcaaaatcc ccattggtct cgacaagcgc cctcttgcag tccatcatcc ccgcgcccgt
    97381 acgttcacga agctccttta ccatggatgc agtgatttcc atcagcagac gtccccccgc
    97441 gtttactcag cgtcctcgaa ttcgtcggca tcatccgact ttgcagacgc ttggaattcc
    97501 ccgttgttgc catccgcgct gaatccctca tcttgttctt cctcggcaag agcctgatcc
    97561 aagccttcac ggccttcgat aacagcatcg gcgatcttgg aagtaagaag ccttacagca
    97621 cgaatcgcat catcgttgcc tgggatcacg tagtccacct cgtcaggatc gcagttggta
    97681 tcgacgatcg caatgatcgg gatgtcgagc cttctggctt cagccactgc aatcctctcc
    97741 ttgcgaggat ccacaacaaa cacagcactg gggagttgct tcatgtcacg tattccaatc
    97801 agacgcttct gcaacttagt cctttcggca ataagctttt ggacttcctt tttgggcagc
    97861 gcgtcaaagg aaccgtcctc ttccatctgc tcaagcttct tcatccactc cacgcgtttg
    97921 cgcatggtgg cgaagttagt aagcatcccg cctagccagc gctcattcac ccaatacatt
    97981 ccacaccgct gagcttcttc agcgatcgta tcgcgtgcct gcttcttcgt gcccacgaaa
    98041 agcaccgttc cgccgtcctg tacggtttca cggacgaaat cataagcctc gtccagtttt
    98101 ctcacagtct gctggaggtc gatgatgtag atgccatttc gcgaagtgaa gatgtacggc
    98161 ttcattttag gattccagcg ccttgtctgg tgtccaaaat gtacgcctgc ttcgagcagt
    98221 tgcttcatag tcaccactgc catgccaatc acctcctccc gcagcggttt cccctccgac
    98281 ctcatcatcc cggagttcca ccagacgccc acctggcgcc cagcaccgcc aaccccgatc
    98341 ataaggtcgt gtgtagtcgc acagcggtta gtatatcaca cgctgaagcg gcctgcaatc
    98401 tcgccacaag ggcgcatcac acagtgcgcg agcaacctgt tgccttgcca ttttcgtttg
    98461 caacagcaac aacgtctcct tttgtcacgt aagcagccag gacttgaccc tcccacttac
    98521 tgcagcccaa acctagcgag cgcgcagcct atcaagttcg tcgaataatt tgtcattgag
    98581 gacccgtatg tatgtgccct tcatcccgag cgagcgcgat tcgatcacgc cggcactctc
    98641 gaacttgcgc agcgcattga cgattacaga cctagttatg cccactctgt cagcgatttt
    98701 ggaggccacg agaagccctt catctccggc aagttcgtca aagatgtgct cgaccgcctc
    98761 ctgttctgaa tacgagagcg tttcgagggc catctgcaca gctgcgcgct ttctcgcctc
    98821 ttcctcggcc ttctcacttc ttgacctgta gatttccatt cctatgaatg ttgcgccata
    98881 ttcagcgagc attatgtcgt tttcgcccag ttcgccgtcg gacttcaaaa gaagcaacgt
    98941 tccgactcgc tcacccccgc catatatcgg agcgacagtc aagcgacacg attctgacga
    99001 tccccccaac atctcattgc agaacgcgcg catcccatta ctgggcctgc gattcgcggc
    99061 tgtctcaaat atcgcgagaa actcttcatt caccttctgt ggcacgtagc cgttcccaag
    99121 gatttcggaa tccaatactt caaaatcatc gtcagtcagg tggggcagag aatggcccaa
    99181 tatcttccca tttttcgcaa caacaaaggc ctggcctgac acggcctctg caagcgcgtg
    99241 ggcaatctcc tcgaaacgtc ctgcatgtcc tggggaactc tggatcagcc tgcctagctt
    99301 ccgtgtgcgc tccagtagtg agttcacttt gagacctcct ctcaccaacc acggacgcat
    99361 gtccgtgcgc ttcagattag gagcaagtta tgctctttta tcttgtccaa acatttgcgt
    99421 ttcatcacac acgccatctg ccggcgctac agaatgtagt ccgaaagatc gctctcagta
    99481 accaactgct ccagcctgga ccgcacatag tcggcatcta ccacaaccct ctctgaatgc
    99541 tccggcgcgt tgaaagatac ctcctccagc accttttcga gaatcgtatg gagccttcgg
    99601 gcgcctatat tctcactgct ggaattgacc tcgtcggcaa ttctagctat ctcttcaacc
    99661 ccatcatccg taaacaccaa atcgaccccg tcagtagcaa gaagagccac gtattgcttg
    99721 gtcagagcgt tcttcggctc agtcagtatt ctcacaaaat catctttggt gaggcttgaa
    99781 agctccaccc ttattggaaa tctcccttgc agctccggaa tgagatcaga aggtttcgac
    99841 atgtgaaaag cgccagccgc aataaacaac atgtgccctg tcttcacagg cccgtatttc
    99901 gtctggacag tcgacccttc cactataggc aggatatctc tctgaacgcc ttcacgggac
    99961 acatcgggcc ctgcgccctg ccctttgacg gcgatcttat cgatctcgtc gatgaatact
   100021 atgccgctct gttccgcacg ggcgatagcc tcttcagcaa cctcatccat gtcaatgagc
   100081 ttcgcggcct cctcggtaat gaggattttt cgagcttcag aaacaggcaa ttttctgcgg
   100141 cggcggcgcg gaggcaacat ctcgcccatg aggtcctgaa ggtttatccc catctcttcc
   100201 actccggccc cggaaaagat ctctatcatc ccgccctgta cgtcatcgat ctcgatctca
   100261 atgatcacat cttccagttc gccccttctc aggcgttccc tcgtctcttc cctgcgcgcc
   100321 cgtgcgctgg ccagtttcga ctcaaacatc cggtcatcct gcggctgccc gacaggcatg
   100381 ccccagaggg cggcaaacgg gttggcctga gaacgatcgc gagtcggcaa cggcgctaag
   100441 gcatccagca gacgctcctc gcccagtatt ttcgcttgct cctgcacgcc cgacatcttc
   100501 tctgctttta ccatcctcac tgaagtctct acgagctccc gcacaatgct gtcgacatcg
   100561 cgtccaacat atccgacttc agtaaacttc gtcgcctcca ccttcacgaa aggagcgttg
   100621 acgagccgag ccaagcgtct ggctatttcg gttttcccta ctccagtggg gccgatcatc
   100681 agtatattct tgggtattat gtcgtcctgc atttccggag ccaacgccag cctgcgttgc
   100741 cggtttcgca gcgcaatggc cacggccttc ttagcggagg cctgccctac tatgtatttg
   100801 tcgagttcgc gcacgatcgc gcgaggagtt aggtcctgca ttacaacgcc tcccgactac
   100861 aattcctcta tgttgatctt tccgttcgtg tatatgcaaa tctcggatgc gatctccaac
   100921 gacttgcgga ctatctccac agcctcaagg tctgtattcc tgagaagagc ccttcccgca
   100981 gccaaggcat atgggcctcc ggagcccacg gcggtgatgg cgtcatccgg ttccaggacc
   101041 tcgccatttc ctgaaagtag cagtatcctg tttccgtcag tgactatcat catagcctcc
   101101 aaccttctca agacccggtc tgtgcgccac tcccggccaa ggtcgctcgc tgcccgaaca
   101161 aggttaccct tgtattcaga gagcttgcct tcaaagcgct caagaagcgc catagagtca
   101221 gaagccgaac cggcgaaacc agccagcaca ttccagtcag acagtttccg gatcttccgg
   101281 gccccgtgtt tgacgacggt atcccccaag gttacctggc cgtcggaacc catggcgcac
   101341 ttcccatcac gaatgacggc tacaacggtc gtgccagtca tccttatcga atctgattca
   101401 ttcattctgt ctgaagcctc ctaatgaact gaaaaaagaa cctatgccct cggatgggtc
   101461 ttgttgtata ccctgtatag cctctctttc gagacactcg tgtaaatctg cgtagtcgaa
   101521 atgtcggcgt gcccgagaag ctcctggacc gcgcggaggt ctgcccctga atcagtcaga
   101581 tgagttgcaa acgagtggcg cattgaatgg ggagtcgccg tctgcggaag tcctgcctcc
   101641 atcaagcgct ttttcatcat ccgctccact gaacgccctg agagcctccc cccgcctctg
   101701 ttcagaaaga gcgccgtctc ttcgctgttg gacgcaaggt gaggtcgggc attggctatg
   101761 tagtcatcaa gcgcccgcac cgccgcttgt cccacgggag ctatacgttc cttagcgccc
   101821 tttccgaaaa cgcgaacagt ccctgacgag gcattgtaat ctttaacatc cagtcccacc
   101881 agttctgcca atcttatccc agacgaatac aacagctcga ggatggcccg atccctgata
   101941 tcaagcgggg aaccgtcgcc acagacggat atcaggcgct ccacctcctg ctgcgacagt
   102001 gtccgaggca gcctcctagg gcgcctgacc tgcgacatgg cgacagtagg gttcgattcg
   102061 ataacgccct cccgcgcaag gaacttcaag tatgacctga tagcgctcat cttcctagat
   102121 atgctgctat gggcaagccc acgggattgg agtgtagcaa gatacttcct gagcatgctc
   102181 agctgataag catcgactcc tgtgcctgca atcccctgcc acgtcccgct cttttcgcag
   102241 aaggatatga attgcccaac atccctaatg tatgcatcca ccgtcctagg agacaagttg
   102301 cgttctgcct ccaggtaacg ggcaaaatcg tctaaatggt ccaaaagaat ccactccaca
   102361 gccgtcccgg caaaatcgcg ctccgcggac acggaacccg acatgacgga gaataggtta
   102421 ttctcgagcc tgggccggga ttgcaatact tgaggccagc gcgcctaaca gccgcccctc
   102481 aacgaacctg caaaatcatg caaagactgc ctcgccaaag caaccttcca catccgtctc
   102541 tcattcttct tgagcgctct cggaggctct gggagcagac caaagttgac attcatcggc
   102601 tgatacctgc ctttgcacgg agtagaaacg taagaacaga gcgaaccgat ggcagtcttg
   102661 ctcgggaacg tcaatggatc ctttcccatg gccaatcggc ccgcattcat cccgcacaca
   102721 agacccgtcg caacagattc catgtaaccc tccacccctg tgatctggcc cgccatgaag
   102781 atccgctcac tctctttgag ttgaagagtt gcgctcaggt gttcaggtcc gttcacaaag
   102841 gtgttcctgt gcatcatccc ataacggaca aactccgcct cttcgaggcc tggtatcatc
   102901 cggaaaacac gactctgctc cccttgttta agccttgtct gaaacccgac catgttaaag
   102961 agggagcccg gcacattctc tctgcgcaac tggacaacag cataaggtcg cctaccggtg
   103021 cgaggatcga caaggccaac cggtttcaaa gggccgtagc gcagagtatc acggccccgt
   103081 ctggcaatct cctcgattgg catacacccc tcaaacagct ggagagattc gaactcatgc
   103141 atctctgctg tctcagcttc aatcaaattt ccccaaaaaa gctcatattc ctccctgttc
   103201 atggcacagt tcgcgtaatc cgaatcaccc ctgccgtatc ttgatgccca atatgcgcgt
   103261 gaccaattta cagaatgacc gcttattatg ggagccatag cgtcaaaaaa gtaaaggtgg
   103321 tcggtgccga gcaacgatgc gatttcttcc gagaaagccg gggacgtgag ggggccagtg
   103381 gcaataacta ccacgccctc ccgaggcacc ctgacaactt cctcccggat cacctctata
   103441 ttcggcgccg cctcgagctc ctttgtaact agggcactga aaagccccct gtcaaccgcg
   103501 agagcctgtc ctgcaggaac acgtgtcctg tcggcgcaac gcattataat ggagcctaat
   103561 gcccgaagct cctctttgag caatccggga gcggtcccct ctcgatcaga cccgagagag
   103621 ttgctgcaga cgagttcggc gaaattgcca gtggaatgtg caggagttga cacgattggg
   103681 cgcatctcat acaacttgac atccgcgccc atctgcgaag cctgccaagc ggcttcactc
   103741 cctgcaaggc cggcgccgat aacgataacc tcattctgca accccggtcc ctccctgttg
   103801 tgcgccactg ccggagtctg cggcctcctt ccatgtatgc ccacattcgg catcagcgca
   103861 tttgtagatc atctcgtcct tcctctgcct cacagtggta aacgagccgc acatcgggca
   103921 actaaccttc gacgggcgat tccatgccac gaaatcgcaa tttggatagt tggaacaacc
   103981 atagaaagtt cgtcccttct tgctccgccg ttcgactaca tccccgccgc atttcgggca
   104041 cttcacgtcc agcttcttca ctatgctccg cctatacgtg catttaggat acatggggca
   104101 agcaatgaac ttgccataac gtccgctttt tatgactagc tcggcgccgc attcggggca
   104161 tgcctcgccg ataggctcgt cttcgatcac aaccttctcg gcgagttctt cagcttcggc
   104221 aagtttctgc gcaaatggct tatagaactc gtcagtgacg ctgacccaat cagcttcgtt
   104281 ctcctctatg ctgtctagct tttcctccat ctttgctgta aactccaagt ccaccacaga
   104341 aggaaaatac tcgctcaaga gatcgcaaac agcaaacccg acctcagttg ggacgaaacg
   104401 tttcttgtca agcgtcgcgt agtccctttt tcgcaaagta tcaattatcg gcgcataagt
   104461 gcttgggcgc ccaatgccgt gtttttccag ttccttgatc aaagtggcct cagtataccg
   104521 tggcggaggc tgcgtaaaat gctgctctgg gaggagctgc accagatcga gccgctgccc
   104581 aacttcgagg gcgggaagct ggcgcttgtc atcgttctta gaactttcat catcacggcc
   104641 ctccgtgtag atcacagtga atccgtcgaa cttcaccttc tggcctgttg cgcggaatct
   104701 gtgctcgccc gcagatatgt cgactgacgt gatctcgtta acagcatccg acatctggct
   104761 ggccatgaac cttgaccaga tgagagtata cagcctgagc tggtcacggc ctaggcttcc
   104821 cttgaggctt tctggcgtgc gctccacgga cgtaggcctg atagcctcgt gggcatcctg
   104881 tgcccctttt ctggaggcat aaactctcgg agatttcgga acatacttcg ctccccacgt
   104941 cttgcgcacg taatctctgg cagctgcctg cgcctcctgg gctaccctgg tggaatctgt
   105001 gcgcatgtac gtgatgagcg ccacatgccc ttctccggca acttcgacgc cttcataaag
   105061 cgcctgggca tttgacatag tgcgtttgcc tgaaaacccc agttttctga acgcctcttg
   105121 ctgcagcgtg cttgtgatga agggaggcgc cggctttctg gcctggtctc taacgtttat
   105181 cgactctact acgtaatcgg ctccctccaa atcagcaatg accgcatcta cctcttcctt
   105241 cgaaagacgc ttgtcttctt cgggaatgaa cctcgcctca aagctgcggc ctctgggctt
   105301 tggagaaagc tcggcaccga gagtccagta ctcctctgac tcgaaggcct ctatctcgcg
   105361 ctcgcgatca catattatct tcagagcaga tgactgaacg cgaccggccg aaagcccagg
   105421 gcgaaccttc ttccaaagga aagggctgag cttgtatcca acaagcctgt caagcacgcg
   105481 cctagcctgc tgcgcctcaa ctctgcccat gtcgattggg cgtggggcct ttatcgcgtt
   105541 cttgacagcg ttctcagtaa tctcgttaaa ctcgatccgg caatctgatt ccggatccac
   105601 gccaagtatg taggctagat gccacgaaat agcctctccc tcacgatcag ggtctgtggc
   105661 caagagtatc ctgtctgcct ttttcgcgga ctccttcagg tcgcgtatca catcgccttt
   105721 gccgcggatc gtaatgtatt ttggagcgta gtcattatcg acatccactc ccaactggct
   105781 cttaggcaag tctctcacgt gaccaactga cgccttcagt gcatactggc gcccaaggaa
   105841 ttttgataac gtctttactt tagctggcga ctccactatt acgagagtct ttcccatacg
   105901 ggcacctcca aagtctggat atcaaaaaca gtttaaccta ggcagaatgc caggtcaaca
   105961 tcgagaagcc ctggagcatg ccacatcagg cgccggccac aacacgtttt ctagcaccgt
   106021 ttgctgtatc gcatgccagg ttcccggtct accaggcccg cgaattccag ccgcgccaaa
   106081 gagaccaaaa gctcctgagc gggcaatccc aagtctgtct gaattctctc aaaacttgtg
   106141 ttcacagtca atgattccag cacagcgcga tccacaggtg caagaacgcc tcctgaaata
   106201 cgagccgtcg ggccatgggt tctgtcaaga agaccccttg atgcaatccg atcaatgacg
   106261 tcgccaatgt cgagcaatgg ttcggcatga gtcgaaagca gctggttagc tcctacagag
   106321 gtcggcctgt tgatatcgcc ggggacagcg aatacatcct tcccatgctc attggcgaag
   106381 tccgcggtga tcttggcacc gctcttctcg gccgcctcca ccactatgca cacctccgac
   106441 atgccgctta tgatcctatt tcgggcggga aaagtgtcct tgctagccgc ccatcccata
   106501 ggaaaaggcg atattacaca tccgcatgcg gccacctccc gtatcagctt tttgagcctc
   106561 cctgtcatat catcgcccat tcccgcgccg agaaccccta ccgtgggtcc tcctccctcg
   106621 atagcaccac ggtgggcaga ggcatctatt ccccgggcaa gcccgcttac tacaccaatg
   106681 ccagcagacg caagcatacg cccgtatctt ctggcaatcg agatgccata agcagtagct
   106741 tgcctggagc caaccattgc agcccaggga acctccgtca attccccttt ccccaacaca
   106801 tacaggacga tgggcggatc aggaatattg cgcaatggcg caggatacag ccgggacggg
   106861 cagggaatca cacgcgccga tatactcgag gccttggcta tctcggtcat ccccatgcag
   106921 agaggatcgt gaacccttcc cgcccaattc cattgggcgc gccgggcata agcaggcggt
   106981 ttcccgccat gttttgcggc atgaaggccc tcgacggccg atccgtagtc ctccagcatg
   107041 cgcaggaagc tccgcgaccc aaaacccggg accgaattcc agaaaaccca cgcagcaagt
   107101 tcctttccct caggctcagc tacattctcc catctcagct taggctcctt atcatccatg
   107161 tgaacacctc gctatctctg ccgaatcgag cgccctgtat tgaagcgctt caagcacgtg
   107221 atcgtctttg atccgggcag aagcgtccaa atccgcgatg gttctggcca cctttataag
   107281 cttgtggcag gcccttgctg aaagggccat cttgtcaacg gcttcataca gcaaggcccc
   107341 ggcaataggc gagagcattg ctgcgtgctc gatattgcga gggtccatgc acgaatttgt
   107401 tacaatgggg ttcccaaagc gccgcctctg cctttcccta gcttcgctca cattggcgcg
   107461 gatcttgtca gacgagcggc ccgcagaatg cccttgcaga tcggcgcggc tcacacgggg
   107521 tgcctctatc tgaatatcga tcctatcaat gacaggaccc gacaacttcc ttctgtacct
   107581 ttgaacctgt gccccgctac acacgcacgg acgctctgaa tccccaagat acccgcaagg
   107641 gcatggattg gccgacgcaa ccagcatgaa cctagaagga aaagcaacct gtacgtgaga
   107701 acgggagata acaacgaatc catcctccat aggactccgc aggcattgga gaactgcctg
   107761 gcggtactca cagtattcat ccaggtaaag cacacctctg tgcgccaatg taacctctcc
   107821 gggcattgga ggattccctc ctcccagcat ccctgcggga gtaatcgaat ggtggggatt
   107881 cctaaaaggc cggctgacgg caaggccccg cccatccacc aagcccatga tgctctgtat
   107941 cgacgtgacc tctattgcct cctcgcgatc catcgctggc agcacagatg gaacacacga
   108001 cgccatcagc gtcttgccgg caccaggcgg gccaaccatg atgatattgt ggccgcctgc
   108061 aacagcgacc tccaaagcgc gcacagtggc ggcttgcccg attacctgtg aaaagtcggg
   108121 ccctagattt gcacccaaat gcgcatgctt agttgagatg tcccgatttg ggactatgcc
   108181 agtctccaga aacctcacaa cctcttgaag agttcgcaca gccaccaccg agacctccgg
   108241 aatgcacata gcttcccccg cattttccac aggcactacc actacggtgc ttcctgcctc
   108301 acgcgccccg gcacatgccg gcaatactcc ccgaaccggc cttattaccc cctcaagaga
   108361 gagctcccct atgaaggtaa tcccttcgag tcctttatga ggggattggc ccgatgccgc
   108421 cagtattgaa acggctattg gaaggtcgag cccaacgccg acctttcgta catcagccgg
   108481 cgcaagattg acggtaatcc tggagagtgg aaactcataa ccagaattcc tgatcgcagc
   108541 cctcacacgt tcgcgagact ctctgactgt gggatcgggc aagcctacaa tgtcaaatga
   108601 aggcaggccg cgcgcaaggt cgcattcgac agtcacagac atgccggtta tgccctgtaa
   108661 tgcgcatcct ctcgccgcag agagcactga catctcctcc aaaaagcatc atgatatcac
   108721 gttcttgata tgtgatagat ttgcatctct aagtccgcag gcaccaaacc caaaagccct
   108781 tatcgagatc acatctacac gcacagtagc gccgccgcac gccgcagaaa ccaccgacgc
   108841 aatctgctta agggaacgga gtttggccgc acttatctgc tcttcaggca ttccataatc
   108901 ccaagtgagc ctcgtcttga cttcgattac caccaaggtg tcgccatcca ccgcgagaat
   108961 gtcgacttct tttctcccaa aacgcatatt gcagccaaga atctcatagc caaggcgctc
   109021 cacgtacgag cgagcaaact cctccccagc cctgcctaca tcctgtgaac ccatccacac
   109081 atctcctcaa ccactatgtt ctgtcccgtg tccgccattt cctcccacaa aagcaaaaat
   109141 cgccctttcg ggcgatactc accatttggg actagctatg tatctacaga ttcatcagct
   109201 gactgactca ccccgcgaaa agtcttcctg tgcagagcgc atggcccaaa agccgccagg
   109261 tttcgcaggt gttcgggagt gggataaccc ttgtgccgct caaaaccgta ttctggatat
   109321 acaatggcaa gttcgcacat cagtgcatcg cgaacgacct ttgccaatat ggacgccgct
   109381 gcaaccgact ggctccttgc atcccctcga accaaagcca ctgcaggcaa gcgcagatcc
   109441 ggaagatcct taccgtccac caaaaccaaa ttcggaacga cagacaactg atccaccgcc
   109501 cgcgacatcg cgcggagaga agccacccta atgttgactt catctatctc atcttgccac
   109561 gcgactccta ccccaacctc aagagcgctt tccatgatct cttctaggac tgcctgcctt
   109621 ttcttggcgg aaaggcgttt tgaatcatcc accccgggca ggtaaaatcc ttcacgcagc
   109681 acaactactg ccgccactac tggtccagca agtggccccc gtccggcctc atccaccccg
   109741 gcaatcacgt ggaaccccgc gtttctcagg gaatcatcga acatggcaat atcgctccta
   109801 acaacttctt cagccattat tccagtcatg gcatttccaa agttgcacga ccgagcttcc
   109861 ctgacctgaa atccctcacc aggatatccg cggctcgcgc catgtccaac tcgcctccag
   109921 ccatcatgca cccacggctc cgagcaacat gcttcagagc ctcatgggga tcgacgcacc
   109981 cctcacacac cccgtatcga ctacgatacg cggccaaacc cacgctgtcg agccgagcta
   110041 taagtcccgt agcaagggct tccacatcgt atatggactc atccaaacag ccgagagcgg
   110101 ccaaggcaaa ccaaacaccc ctatcatcga cgcgaggcgg catgatccca ggagtgtcaa
   110161 gcatctcgag attgcggtcg atcacgatcc actgcttagc ccgggtgacc ccggggcgat
   110221 cacccgtttt caccctcgct cgcttagcgg ccctatttat gaatgaagat ttccccacat
   110281 tgggaatgcc caacacagct gtgcggaatc gaacacgcga aggtcctctc tgaacgtccg
   110341 ggcccgctcc ggccttggcc gcggcagacg ccgcggcgat gacctccgct acaccttcgc
   110401 ctgttcggca attggtagga acggcgcaaa ttcccgcttc cgtgtagcaa tcgacccatg
   110461 ctctagtggc aactggatct gccagatccg ccttgttcag caccacgatc ctgggcttgt
   110521 tgccgacagc ctccgcaaac tccctgtttt ctgtagatct cggagcccgg gcatcaagaa
   110581 tctcgatgac tacgtcgatc agcttcatgc tctgcttgat ctgggatact gccttggcca
   110641 tgtgtcccgg ataccaacta aagcccattg caattcctcc aaaataaaat aacgccgggc
   110701 ccggagagca tccgtcccgg cgatgcgcat tgccatcaag gcatgcccgg cgccgcagcc
   110761 atgccggatt ttggagttcc gatcgcctcc aggcaagttg gattcttaag cgttctaatt
   110821 cgatccaccg gccaatacac cacaaacgcc ttcccttgga tgctgccaaa gggcaccatg
   110881 cctacagaat catcagtgct gtcttgacta ttgttcctat tgtcccctag tacgaacact
   110941 ctcttcgttg gaacaagggc cgggccgtac tccctagtag aaggctcggc tatatatggt
   111001 tcattcaaac ctgttccatt tacgtaaacc atgcccccgc tgatcataag ctcatctccg
   111061 gggacaccta tgacacgctt gatgaacatc tgtcgcgggt cttggggata ccgaaatacg
   111121 ataatgtccc cacgctgtgg ttcgcggaat ctgtagatga acctgttaac taggagcctc
   111181 tcgccattat acaaagtagg ctccatggag cttccttgaa ccacaaatga tctggcaacg
   111241 aaagtcatga tgaaagccgc cagaaccaca gcaacgatac atgcgttgac atactccatg
   111301 atcacggatc ccgtcgaacg cgtctcctca tgcccttcac aggggagaac ctcgtccagc
   111361 acggatccat tgccctggct ttgggaatcg acgtcgctcg cctcccgatc caggccagct
   111421 tcggtgtggc tcaaccgagg ttccattctt tttcggggag aattgctcat gcgttcataa
   111481 cctcctcgcg cgacccacgg ctagtcaaat cagccgccat gtcaagcgcg gcgagtctgt
   111541 cactagtgct gcctcttctc ccgaatccta gccctcttcc cgacgcgacc gcggatgtaa
   111601 tacagtttcg ccttacgcac atcgccttgc ctgactactt ctactttgtc tatcttaggg
   111661 gagtgaaccg gaaagatcct ctcaaccccg atcccagatg acatttttct cacagtaaaa
   111721 gtctcggaca ggccacttcc gcttctacct attacaacac cctcgaagac ctgtatgcgc
   111781 tcgcgggcgc cttccacaac gcgaacgtgc actctgactg tatccccagg gccgaagtca
   111841 gggaggtcgg aacgcatctg ctcgcgctcg attgtatcga tgatgttcac ttctatccca
   111901 ctcctcaacg ggttttcgac aaacgtcagt ataccataat gcaactgcgc tcatcaagga
   111961 agaccgactg ccaaccggaa ccgttattag ccatcctcca tgacttgctt gagcaactcc
   112021 acatcttcat cggtcagctc gacttgactg agaagatccg ggcgctgctc caatgtaacc
   112081 ttaagcgcct gttttcggcg ccatttcctt attctctcat ggtctccgga aagcaacacg
   112141 tcgggaactt catgcccacg ccaaaccctc ggtcgagtgt aatgaggata gtcgagaatc
   112201 ccatcccaaa acgaatcagc cgaggcgctt tcctcgtccc ccaagacgcc gggaagtagc
   112261 ctagccgtgg catcaacgat gaccatggca gggagctctc cgccagtgag cacgtagtcg
   112321 cctatggaca ccctgtccgt taccaggctc atcacacgct cgtcgacgcc ctcataatgc
   112381 ccacagatga ttaccagatg atcacaagac gcaaacccct tggccatgtc ttgcgagaat
   112441 gtcgagcctg cgggcgtagt catgatcacc cttgaccggc tctccttggc ctgcacgcgt
   112501 gcagcaaggg catgttcgac agctgcagca atcggctccg ctttcatgac cattccaggt
   112561 ccaccgccga acgggtaatc gtctacaaca cggtgccgac tggtagaaaa atcgcgaatg
   112621 tcgtgaattc ggacagtgag aatccccttt tcctgcgcgc gccctaggat gctttcagac
   112681 agtggcccat agaacatccc cggaaaaatc gtcaggatat cgatttccac gttccgaatc
   112741 acctacccat gctcaatcca tcccgcatcg acaagcgccg atgcaagatt actattggca
   112801 tcagcgcaaa ccaggcctat cgtctatgat catacgcctt tctgcgacat cgatctcgca
   112861 gacaatgtcc ccaaccgccg gcaccaagaa ttcccgcccc gaatcaggct ttaccacgaa
   112921 aacatcattg gccggcaatt cgattacttc cttcacgatg cccaggcgtt ctcctgtctt
   112981 agcatagacg ccgaggccga ccagatcaaa tgcgtagaat tcgtcttcag gcaactcagg
   113041 cagttcctcc acgggcagat aaaggccgaa acctttaagc agttttccgg tttccacatc
   113101 ctccacgcct tcgaggagca ccagcgaagc ctcgccatgg gcgcgctccg atatcacttt
   113161 gcgctcctga aaatcggcac agttctcgcg tccgaggaaa acgcgatcaa tgccacagaa
   113221 acgtctgggg tcgccatttt cggggcggac tctcagctca ccccaaagtc cgtgcgtccc
   113281 catgattacc cctatgagca ctagctgatc gccacttcga gtcaatctac gatttccacc
   113341 atcacacgct tgccatctct aatggcagcg gcctttgtaa cagaccggat tgcacgtgcc
   113401 acacgcccgt gtcttccaat taccttgccc atgtcgtcgg gagccacaga tacctcgaat
   113461 atgactgaat caccgctttc aatctcgcga acgcgcactt cttcaggatc atctacaagg
   113521 tttcttgcga gaaatgtcac gagttgacac agtctgcctt cgtttttctt gtttcctgcc
   113581 atctctatca ccggttagtt ctcagcagca tcgaccggcg ccacgtcggc ctctgcagac
   113641 tgagttcctt ttccggatgc tccagacatg tcgagacctg ccttgaccag taacgcgcga
   113701 gcggtctcag aaggctgagc tcctcgtcgt atccactccg cagccctgtc tacatcgaca
   113761 acgagctccg gcggatccat tctgggattg taatgcccca aaatctcgat gaatcttcca
   113821 tcccttggcg atcttgaatc agccactacc aacctgtaat gcggttgctt ttttgctccc
   113881 gtcctagtta gcctgatacg aacagccatt tccttatact gcacctccga atggttacta
   113941 ctgaacaaaa agcctaaacc gaactgacgc ccctatggca tgaacggcat ccgcgctggc
   114001 ggcccgccac gtttagccgc atctccgaac tgtttcagca ttttgcgagt ctgctcaaac
   114061 tgcttgagga gagcattgac gtcctgaacc ctgactccgc ttccagctgc gatcctccgc
   114121 tttcgagacc catctatgat gccaggattg tgcctttccg cgggagtcat cgatcttacg
   114181 atcgcctcca cccgcgcaag atccttttcg tcgatggcta cgcccttgag cgccttcgat
   114241 ttccccagcc cgggtatcat tcccaataat tgatcgattg gtcccatttt ccgaacgtcc
   114301 tgaagctgag aaaggaaatc ttcgagtgtg aattcctgct tgcgaagttt ttcttccatc
   114361 ttcatggcct gttcagcatc gaaagcgtct tgggcctttt ctattatcgt aagcacatcg
   114421 cccatgccaa ggatgcgcga ggccatccgc tccggatgga attgctcgac tgcgtcaagc
   114481 ttctctcctg tggttacgaa cttgatgggt tttcccgtaa ctgcacggac agaaagcgcc
   114541 gcaccgcccc gagcatctcc atccagtttc gtcatgatga taccgtcaat tcccaggcgc
   114601 tcgttgaaca tggaggccac attgaccgcg tcctgaccgg tcatggcgtc aaccaccagc
   114661 aggatttctg tggggttgac tctggacttg attctcccaa gttcctccat catctcatcg
   114721 tcaacatgaa gcctgccggc tgtgtcgagt attacgacgt cgtatcccat ggactttccg
   114781 cgttcaacag ccgcagccgc aatgtcaacc gggtcagtct tgtcacccat tgagaaaact
   114841 tcgatcccaa gttgacctcc gagcacctga agctgtttga ttgcggccgg tctatacaca
   114901 tcgcctgcca cgagcaaggg cttgtgcccc tgcttgcgca ggaggtttgc cagctttgcg
   114961 cagctagttg tcttgccgga tccctgcagg cctacaagca tgatcactgt cggaggacgg
   115021 ggcgcaaaac tgatctttgc cgccgtgcct cccataagtt cagtgagctc ggacatgacg
   115081 atcttgatga cctgttgagc cggagtgagg ctcttcatga cctcctctcc gaccgcgcgt
   115141 tcccgcaccc tagcggtgaa atcacgggcc accttgaagt tgacgtctgc ctcgagcaaa
   115201 gccatccgaa cttcacggag agctgcatcg acatcctttt cagttagtcg cccgcgattc
   115261 cgcaggttac cgaaaacgtc ctggagacgg gctgagaggt tctcgaacat tatgccttgc
   115321 ccctcctcta gtcatcttcc atgccagggc tgccgccatc tcctgaatcg atagatgcca
   115381 ccagccgccg ggccgaagca atgatctcca tagtctgctc gatgttccgg cgcacccctg
   115441 ccaaatcagg accgcttggg cgctgggcac tgcgctcggc ttcatcgagg cttgccatag
   115501 ccatttccag ctgaaaatcg gcatcagaca gctgggaatc tagttcgcca acgcgctttc
   115561 gcctctcttc agccattctg agaagaccga aggtctcctc atggccctcc atcgcacgct
   115621 ccgcacgggc aagcgtgtca tggaccgcct gtcgggatac cccataaagc tcggatatct
   115681 ccgcgagcga gtagtcgttc aagtagtaga gcacgcacat ttcccgctgg tgttcggtaa
   115741 gcagaggacc atgaacatca agaagagcgc ccatcctgag catcttgtcg agcatcggcc
   115801 ccgaccacct tgcaagtgtt aaggtctata cctttacacc acgcagtata cagggatgag
   115861 cgacccttgt caatatggac ttcgcgtatg gacctttgaa gctcgccaga gcatcaagca
   115921 gcatgctcac tgccgcagca caggattact cagcctgcag acattccgcc ttcaagcgtt
   115981 tcaaaccact tgtgcctata aggaccaaaa tcctgtgtac ggttacgcag acggacgcct
   116041 gcacaattcg gaaggaaagc ggccccgcag ggccgcaact tctaaacaag tctcaacacg
   116101 tttcctggca atccccggat ctgcattgcc cgcaattcgg agcctagctg ctcttttaga
   116161 ctaaggtatt cttgctagtt actcagcgtt tggcaccttt ccggccatca tcacctgcgt
   116221 ccgctctaag aagcccctga acaaaccgtt cgggactgaa aggtctgaga tcttccgccc
   116281 cttcacctac tcctacatat ttcaccggga tgttcaattc ctggctgatg gcaaccacga
   116341 tgccccctct ggctgttccg tccaacttcg tcaagaccac gccagtgact cccaccgcct
   116401 cattgaaaac ccgcgcctgc gctatggcat tctgtcctgt cgtagcgtca actacgagca
   116461 agacttcatg tggagcttca tcgcactccc gcgagatcac cctcttgatc ttcttcaact
   116521 cttccatgag gttgctcttg gtgtgaagcc tgccagcggt gtcaacaagc aggtagtcgg
   116581 agccacgggc ccgagcagcc tggtaggcat cgaaagccac agccgccgga tcggatccct
   116641 cctgatgtcg tactacattg cacccagtcc tgttacccca aacctccaac tgttctatgg
   116701 cagctgcacg aaaagtatca gctgcagcaa gcgtcactct gttcccctca tgagccaaca
   116761 tggacgctat cttccctatc gatgtagtct ttcccgtccc attgaccccg accaccatca
   116821 ctacggtcgg tggctcgtca gcccttgcaa gaggcgcggt cctttcgcca agtatcgaga
   116881 tgagctcctg ctctatcaag gccacggccc cgtcggcatc gacctgctct ccacgcgaac
   116941 cggattcccg cattttgttg attatcgcag tggtcgctgc aacgcccagg tctgcctgta
   117001 tgagaactgc ctccaattcc tcaagggtct cctcgtctat gaccttcttc ttggagaaca
   117061 aggcctcgat cggatcgata atggcatccc tcgtctttgc gagtccactc caaaggtttg
   117121 agaaaaatga cggtgacatt gacatacctc catgccccta gctgacctgc tgatccgttc
   117181 gaatcgagaa gaccttcgac actcccgact cttccattgt cacgccaaac agcgcgtcag
   117241 cacactccat ggtccgtttt ctgtgggtca caataattac ctgcacgtcc tcggatgtcg
   117301 tcttgagcaa ctgggcaaac cgtattaggt tagcctcgtc gagcgccgaa tcgatctcgt
   117361 caaggagaca gactgggctc ggattcactc tcatgatcgc aaacaacagg gcaatggcaa
   117421 ccagcgacct ttcgcccctt gacattagag aaaggctgga gagtttcttg cccggaggct
   117481 gacatactat atcgatccca cactcaagag gattgtcagg atcatcaaga acaaggtcgg
   117541 cggttccacc gccgaatatg tcggtgaata tgtcggcaaa ctccgatctg accgcctcga
   117601 acgtctcaag gaatctcttc tcacatgtaa gctcgcattc ccgaactacc tcatcgagtg
   117661 aagcctgcgc agcctccagg tccttcaatt gagtttccaa gaagccgtgc ctctcagcaa
   117721 gcttgcggct ttcctcaatg gcggtgtggt taaccggacc gatttcagtg atctcggcac
   117781 gcagttgcgc gatctctgcc tgaaccccgg ccctagactg aattgcaaca tctctggcaa
   117841 gtccttcctc ttcagatatg gaataggcag aagccagccg ttcaacacat gcctcgtatt
   117901 ccgctgcaat ccgcgcccct tctattctag ccccgttcag tttctcagcc acctcttggt
   117961 gccgtttccg ggccacacgc gctgccaact cgccctcatt gacatcagca gctgtcttcg
   118021 cccgctcctc acggccctcg tcgagcagac gctgggcttt agaatactcc tgcgcaagag
   118081 ccgcagccgc ttccccaata gcctcaatct gcgcagcagc gtcagttgct tcctgacaaa
   118141 ctctattctc ctcattccgg agcctagcaa gctcctcgtt atgggacatc agttgctcct
   118201 cggctttgag cagagcggcc ttaagcccca acagcctctc gcggtaagta gccgcctcct
   118261 gcgccagctc tgagtagcgc tctgcctctc gtgattcatc ttccttcaag ccggccgcgg
   118321 actcggctaa tacttccaat gactcttcta gtttctttcg acgggcctca gaccctgaca
   118381 actgttgagc aaggctcttc gcctcctcag tcaacgcatc aatctccctg cgtactatct
   118441 cagcttcaag ttccacagtc tcaagctcgt ctgcgaaccg agaaagctca gcctctgcca
   118501 atcttatttt ctgaacgaca ttgctcagga ccgactcgag agtcgcctta ttgcgttcat
   118561 tctcctccca caccaacctg agagcgccgg cttctgccac agcctcgtcc ttgagcctat
   118621 gtatactggc gctctctgtc ctcgcactct cgagttcagc agcaagctct tcaagccgtc
   118681 tggaccgcgc aagcagtccc ccgcgcctat caggtgcctc tccgcccgtt atggcgcctc
   118741 cggcggagat gatgtctccg tccaaagtca ccatgcgaca cctcagcccg cttttggcaa
   118801 gccggatgcc gcatcccaag tctctcacca cgactacgcg tccgagaagg tactcaacca
   118861 cctttcggta gatgggcgcc gattccacaa gatcagccgc aagcccgaca attccgggct
   118921 cagccacagg aagctgcgcc gaagaaatgg gggaaggctt caagatgtcc agcggaagaa
   118981 aggtagcgcg tccggcacgg tttttcttga gaaactccac agctcgctcc gccccttcag
   119041 aggtggatgc cactatgttc tggactgaag ggcccaatgc tgcttcaata gcgatctcgt
   119101 attccttcgg gacgcgaatt agatccgaaa cagtgcccgc aaggcccata gccctcccgt
   119161 tatcaccagc caaaagcgcg cgaactgcgc ggccgtatcc ctcatactgc ctctgtagcg
   119221 catctatgct ggagtgcgcg gcagctgccg acgaaacctt cgcctcggcc tccatggcac
   119281 gctccgaaac ggattccgca gcttccatgg cacgctcata taataccttg gcgtccgcag
   119341 cctccctctc cgccttttca agatcgcccc ggagccgttc taattccgag aaaagatcat
   119401 cggtctcacg cctggcgcta tcctgagcta cagccttctc ctgggcttgt tgtatcaaac
   119461 gcgccaaccg agcttcgttc gaccgcttcg acgactcaat agcgccaatg cgcgatctca
   119521 agtcagattc caaagccaaa gcgtcaaaaa ggtccgattt tgcctcctca acccttgctt
   119581 cagcgtcagc tcgagccttg gacgcctctg ccaaccgtct ccgcgatgca tcagcttgct
   119641 ccatgacatc gcgcccctca agacctgcgg catcaacctg accttgcaac agcgcaaatt
   119701 cattttccaa cctggcgtgt tttccctctt ccgcctcgca cgccgctgcg atctcctgag
   119761 aacgttccag gagagttcgc ttcctctcct gggcaagctt gagtctaccg ctttccctct
   119821 cagaagccgc agcagcctga cctgccgcaa catgtagcgt ctccacagcc aagtcagctt
   119881 gcattgccct agtttttgcc gactccagca cagcctcgca ttgggcggac tcggcggcac
   119941 actcatgttc tcgcctttca aggtcatcta ttcgtgacgc actgtcgagg cgacgaagcg
   120001 agagcctctt gagatcgtgg cagagaaggt ctatctccag tcgggtaagc ctttcggtaa
   120061 gctcgcgcca gcgttctgcc tcctccgcct ggttccgaag gacaggcagc tgcctaccga
   120121 gttcagcaac tagatctccg accctgagct ggtcagcgcg cacctccacc agctttcgtt
   120181 cagcttcccg cctccgtgca cgatacttca tgattccaga agcttcgtcg aaaatggccc
   120241 gtctgtcctg cgagcggtcg gacaatatga tgtcgatctt gctctgttct actacagagt
   120301 aagagtcttt tcccagacca gtcccggaga aaagatcgat tatgtccctg agccgacatg
   120361 gaactccgtt caatacatac tccgattctc cggatcggaa aagccttctt gtgaccgtga
   120421 cctcagtgaa ccccaggttc agaaagtcat ctgaattatc gaatacaaga gtaacctctg
   120481 catagccaag gggtttcctg ctttcagttc cagcgaagat aacgtcctgc attcgcgacc
   120541 cacgaagaat ccgcgggctt tgctcaccca aagcccatct gagggcatcg gcaatattgc
   120601 tctttccaac accattgggc ccgacaactc ccgtgagccc aggcgaaaaa tccaagtcca
   120661 ctctgctcgc aaatgacttg aagccgaaca gagagagctt tttcagatac aagttcggcc
   120721 ctcccatgac gtacactcag caaaagccgc gccaagcggc gcagcctttg ccgattgatc
   120781 tttcaaagtt agtattgccc gctaccgcgg catcacgatg aactttatag ccgtgcgttc
   120841 ctctccgtct atttcgatct cagaaaaggc aggcacggtc accaaatcga ttccgtttgg
   120901 cgccacgaaa ccccttgcaa tagttattgc ctttactgcc tggttcacag ccgctgcgcc
   120961 cacagcattc acctgcaccg aaccttggtc cctgacaatc gcggccagcg ccccagcaac
   121021 agcttttggg tttgactggc cagatacttt caaaacctcc aagtcgcact ccccccgaaa
   121081 cgtacatata tgccgacgca caagcgccga ctgtctccgc ttcgccattg tcgccaggca
   121141 caactggaga gttctcattc atcgtcagaa gcgcagttgg cccatgcaga ttcgccgttc
   121201 tcgagcattc tcagcccatc cttggctgct tcctgttctg cggcgcgttt gctcggacct
   121261 tctcccgttc caactaccct gtcggcaatc aacgcctcca ccgtgaacca gggtttgtga
   121321 ggcgggcctt cgctcttgac cgttctgtaa gatgcctggc ctaaggatct ggcctgcact
   121381 gcttcttgaa aagcagtctt gtggtcgttc tcaggaccgg taatagcatc atggatttcg
   121441 cttgccagtg cccgaatgat gaaagatctg gcctcgtcga agccggagtc gagatatacg
   121501 gcgccaataa tggcctccat agcgcctgcc aagtttctct gtcgacacct gccgccgctc
   121561 gattcttcgc catgccccaa cattataaaa tcggagactc caagatcgac ggcggcccgt
   121621 gccaaggcct gcccgctcac tgcctgagca gataccttcg acaacctgcc ctcgtctgca
   121681 tcttccatga gagtcatcag ctgctccgcc ataacggctt tgagaatcgc atcgccaaga
   121741 aactcaaggc gctcgtttga taaagcccga tcctggccat gctctgctgc ataagacgta
   121801 tgggtcaacg ccctaactag aagagatttg tcttcaaact cgaggccgag aacagccgaa
   121861 accttatcga tctcgatcgt cgcacacacg gttgtatata tagtcgacag cctgacctac
   121921 agttctcatg gaatcgaagt cttcgtcggg cacacgcagg tcgaattcct cttcaatttc
   121981 gtacacgatt tcgttgaggt acagcgagtc ggcgccaaga tcgtcaatga aatccgactc
   122041 gcgacagatc tcctcctctg caacaccaag cttgtcggag acgatctcac gaactttagc
   122101 aagtatttca gcttcagtca aaactgacca cctccatgca aggatattca atcccatctt
   122161 ataaagcaat taccttcatt tcaagcccgt acccaatacc attgcgtcca gtctcaagcc
   122221 tcgccgtcaa ccatggccga aagagactgg gacatcttct caacaagtcc tctatcaagc
   122281 agttcggaag tgactctgat agcgttggca aacgccttgc gattggagct tccatgagcc
   122341 ttgacaagca gaccggacag gcccaagaga ggagcgcccc cgtattctga ataatccatc
   122401 ctcctcttga cactctttag cgcaggtttc acgagaagag ctcctgcctt caccatgcag
   122461 cccttgctca tctcctcttt caccatgtcg aaaatggctg atccgagacc ctcagaaagc
   122521 ttgaggacga cattaccagc aaaaccgtcc attacaacga cgtcagcttc tccatagaag
   122581 atcgaacggg cttcgatatt gcccacgaag ttcagccccg acccggacag catctgattc
   122641 gcctggacaa tcaccgaagt tcccttgcta gcttcagtgc cgttcgacag caaaccgatg
   122701 ctgggattat ctattccgag aagcgccgaa gcataagctg atcccataat cgcgaactcc
   122761 aacagattct tcgcttgaca ctccggatta gcgccgatgt caaggataac gcaaggatga
   122821 cctccaacgg tcgggatagg cgttgccaga gctggcctcg atgccccctt aagccgccct
   122881 acaacgagag gcccggccgc aacgagagcc cctgtgctgc cagaagtgac cgctgcttgc
   122941 gcctttttct cacggacaag cttagctgct acgaccattg aggagtcgcg cttgtggcgc
   123001 acagccgcaa gagggtcctc gtccatggat atcgagtctg ccgcgtgcac gatctctatg
   123061 tcagattcat cccgtccgtt ctccttcagg tgcgccttta tcgtctcctc gtcgcccaca
   123121 aaaatcactt gggccccata ctgatcacgg gccatcaacg ctcccgggat catctcagcc
   123181 ggccccatgt cgcctcccat gacgtcaaca gctatccgca tatggataac cccccaaaaa
   123241 agctcaacaa aagctgaaag cgggttcagc cgaactacgc gcgtccgaat gaatccgctc
   123301 tgcaccctta ggttgaagaa ttactctgaa acatcaataa ctgtgcgacc cccgtaatat
   123361 ccgcactccg ggcatgccct atggggcagc ctgggcttct tgcactgtgg gcaaacggag
   123421 agggtcgggg cctcagcttt ccagtttgcg cgacgcttac gagtgcgcga cttgccggat
   123481 ttctgttttg gaacagcagg catattcttg gtttcgtcga ggcacaaacc gcgcgccccg
   123541 aggaaacctc tccacctcca ttcaatcatg attaagctat gatattgtac tactcttgga
   123601 cgaagcaggc aagttttctt cgccaggtct ccatactctc cgtgccgttc gagggcagga
   123661 gagtatcgcc aaccgtgtca gcatcaaagc cctaccacat ccagaacgaa acagtgttgt
   123721 tttcactctt tgtggataat ctttgccctc tgaaacaagc cccagctctc atcgggaggg
   123781 cacgaacaat cgccccagtt caagttgtgg ccacattgcg ggcacaaccc cttgcattct
   123841 tcgtcacaca ctatcctgat tggaagcaaa agaatgaagg cttgctcgat tgccggggct
   123901 atatcgacaa tattatcctc cccatactcc cgctcatctt cgtcggggga atcaggggcc
   123961 tcgtcggatc tgtagtaagt ctccagaatg gacgattcca aaggcaactc aaattccgat
   124021 aggcatctgt cacacgttgc acgcgcgcga ccgtatgcta tcccagttac aacgattcgg
   124081 cgtcccgtat tagtggcttg ccccacaaat ctgaccggtt caacaaacac gagctccgag
   124141 cgaatcagat caatcgaagg gaaatcctcc gccatgtcaa tcgacatatt ggaccccttt
   124201 tcctccctga gcccctcaac atctatctgc acctcaatac cacccctgcc gggatcaagc
   124261 tcccagcgcg ctaacgatct ccgcagtgtc gcgcgcgatc atgatctcct cgttggtagg
   124321 tatagccatg acagtcacgc gagactcggg cgttgatatg atctgctcca caccccttgc
   124381 actattcttt ttctcgtcca gttcaatccc gaacagctgc agccgatcac atattcttct
   124441 gcgcatgtcc gacgaattct cccccacccc agcagtgaat actattccat caatgctgcc
   124501 catagcagca gcgtaagctc caatgtattt taccacccta taagcaaaca catccagggc
   124561 cagtctggcc aattgatgcc cttccgcagc ggccttctca agatcccgga aatcgttgga
   124621 tagccctccc gaaaggccca gcactcctga gtttttgttg agatacccca acactatctc
   124681 gctcgcgctc ttgtccagtt gctcacagag gaaagatacg acggcagggt ccagatcgcc
   124741 agatctcgtt cccatgacca gcccttccaa gggagtaaaa cccatgcttg tatcgatgga
   124801 ctttccccgt ttcaccgctg ctatgctcga tccattccca agatggcatg tcaccaggga
   124861 gagttcttct ataggccttt tcatgaggat agcggcacgt tgtgagacgt atctatgtga
   124921 agtgccgtgg aaaccgtaac gcctcactcc atggttcaca tacatgtcat atggtaagcc
   124981 atacatatag gcctctttcg gcatggtctg atggaaggca gtatcaaaga ctcctaccat
   125041 tggcagatca ggcatgagcc tacggcaagc ctcaattccc attatgttag gcggattatg
   125101 cagaggtgcc aggtcctggc attcatgcaa agctttcatg acttcttcat ctatcatgac
   125161 cgaactggca aacctttcgc caccatggac taccctgtgt ccaatcgcag agatatcaga
   125221 caggctgcct attactccag tctccggatc tacaagggcg tcaaatacgt atttcaacgc
   125281 gcgctcatga tccggagcat ctacatcgcg ctttaccttg tcatgtccga tccgtgtatg
   125341 ggtcatgaac gcatccggcg acccgacccg ctccacgcct ccacgagcaa gacacttctc
   125401 ctcatccatg tcgaacagct gatacttcaa tgatgaactt ccacagttga ttaccaggat
   125461 cttcattaca ccgatgacct cctaaccttt gaaaaagaaa acatggcata caccatgtta
   125521 ggcaggcacg cccctgacga gttgacgcga gcataagggg gctctggcct acaaaccggc
   125581 cgcaacattg accatcctct gatgatcagg atccagcaaa gcgctggcat gggttcgact
   125641 gcccaagaaa tgaacacaga agtggccatc gaagttgttg tccttgataa ggcaacctcc
   125701 gtgcggcatc ccattgatcg aagcggcaat tcgtctgtcc ccgactaata cgataacagc
   125761 tcttctggac caactccatt ttccgccgta tatctgcttc agtatcgcag tatcagccgc
   125821 cgtcaacggt tcagcgtcag cgtgattatg tcctccacgc ctctgtacct gaaacgagag
   125881 ccctgtagca caatcaatga tagtagcccg ggcggtaaat atagtcttcg ccacatccca
   125941 atggagaagt tcggcgttgg ctctgcccga ggtgccgcgt gaggctgcag acgcgcaaat
   126001 tgggatcttc agttcctggc caggtttgat cagttcaggc cgcaagaccc cagaggccgc
   126061 cgctatctct tccacagtca ctccgaattt cttggcaata gcatacattg tgtcgccctt
   126121 ggccaccaca tacttgctgt atgatgccga ctgtgccttc tgtgcaagag agctccatgt
   126181 gttagggccg acgatgccgt ctggcgtcag accaacatca ttctgaaaac gaatcactgc
   126241 ggcataggtg gcgttgccaa aaaccccatc ggcttcaccg tcgagatagc ctgcaagcgc
   126301 cagttgctct tggagttcgt acacgttttc gcctcgatct ccctttcgca gcgtgacagc
   126361 agaagcgttt gcccccggac atgcccaaag aagcaataca acgcagaacg cggccaacag
   126421 aggcagcaaa atcaagcgaa accgatgcca cacccttctc gcctcccaaa ataacgcctt
   126481 acccgtattt ctaccgaacg ccgtaaaatc cttcagcata tgcaggagct ctggcaaata
   126541 tagatttgag gacgacccac agcacgaaag gaagtgcatg ctcctatgaa gccgagcttg
   126601 gaaccgaacc ggcgaaatgc atatctcgtc gccgcattgg cctcattcat cactatcgct
   126661 ataatcgctc tccctgaaca agcgtttcaa gcttccctag atggcctacg cctctggtgg
   126721 aacatagtgt tccctgccct tcttccattt ttcatcgctt cagacacctt gatgggcctc
   126781 ggcgtcgtgc acttcatcgg ggcactccta gagccgctca tgaagcccct attcggagtt
   126841 cctggagtag gagcattcgc cttggccatg gggctcgcat ccgggtatcc tataggcgca
   126901 cgcataacag caaggctccg aagagaaggt ctgtgttcca gggttgaagc cgaacgcctt
   126961 gtctctttca ccaataccgc tgatccgctt ttcatggtgg gcgccgtggc cgtgggaatg
   127021 ttccaacttc ccggtcttgg cccgattctc gcggcttctc actatgtttc agctatactc
   127081 cttggctttc tgcttaagtc ctatggagag agcgataaga ggagaaagac agaagaatac
   127141 cggagggaag agtcgctgct ctcccgtgca gcccatgaac tgtacactgc taggagtcaa
   127201 gacaacagat cattcgggca aatcttcgga gacgcagtga aggactccgt aggaacgctg
   127261 cttctcgttg gcgggtgcat aatgatattt tccgttgtga ttcgcgttct cacggtgttt
   127321 cacatcacag acttgctctc cgcgccactc atagctgtgc tcactccact cggcgtccca
   127381 caggatttgg tgacttcgct cgtaggcggt ttctttgaga ttacaatagg cacagaaatg
   127441 gcaagccgcg ccaccgccag cattgcccag aaggcaatag cagcgagcgc aataatcgcg
   127501 tggagcgggc tttccgtatt cgctcaggtt gcgagcatgc tctttggcac tgatattcga
   127561 ctcacgccat acttcttcgc cagaatagtg catgcagtac ttgctgccat agtgacagct
   127621 atcatgctca agatagtccc atgggccgcc cagcagacaa tagccattcc cggcctctcc
   127681 agctcgcccg gtttcttggc cacagtcggc tactcctgca caatgcttgc caaaaccgca
   127741 gccatagggg catccatcat ggcggcgcta gcgcttcttt ccaagccccg ggcattcggc
   127801 ttcagagtga aaaaataggc aatcgaatta cctagcctcg ggattgtcct tcctgcgcct
   127861 cagctcttct atgccttttc ttactacaaa cgatgttcta tccagagttt cttgaagaga
   127921 agcgagggta gcctcggcat aatcgtccgc tccaagcctg atctccatag cttcgcgttc
   127981 tgcacgcatc aacagcacct ccgcctcctc cctcgcgctg gcaaagacct ctccatcggc
   128041 gccagaagcc tgtgctgatg cgatcccaac gccatcctcg gcagccaacc ggcttctctc
   128101 ggctatcagg gcctctgtct gcctcatctc ttcaggcagc gcaatccgta tcctgtcgat
   128161 aagctcgaga agctctactc cgtcaacagc acgttttccc gaaaaaggca gctttccgct
   128221 ggattcgatt atcgtctcca tccgattcag gcattcaaca accgattgtt tgatcataat
   128281 cctacccctt cctcctacac ctggccgaca cgcgttccac caatggctca ggcaccaggc
   128341 cgtgaatgtc tcctccagag cctgctactt ctttgacaat gcttgagctg aggaatgagt
   128401 attccataga tgtcattatg tacatggttt ctatggacgg atcgagtttt ctgttcatca
   128461 aagccatagt cagctcgaat tcgaaatccg acactgcgcg aaggcctctt atgacgacat
   128521 gtgcaccaca agcccgcacg tagtcgacca aaagcccttc gaaatgatca acctgcacat
   128581 tggggagatg ggcacaagca accctcaaca tctccaacct ttcttccagg gagaacatat
   128641 ggtctttagc ctggttttcc aggattgcga caataacatt atcaaagatc gatgctgcac
   128701 gcgttataat gtcaaggtgt cccatagtta ccggatcgaa actgcctggg catactgcaa
   128761 gggtcaacag ctatccctcc gacgagaaca tatagaaact gacttcggta tctccgtagc
   128821 gctcctgcct caccattgtc atccttgagg atccatatgg agcgatatcc cgtgatgagt
   128881 gttctacgag aactaggcca tctcctgcga gaattcgcgc attctcgata agggacaaca
   128941 acttttcaga agcctccata gcatacggtg gatccacgaa taccagatca tatggcgcct
   129001 tctctgatac aggtccccta cccgcaccga tggcgcggaa cgcgtcggcc tctacaactg
   129061 tcgacgcctc ctcaaagccc aagtcatcca gatttctttt tatgacgcca caagcagcat
   129121 ggtccttgtc aacaaacacg cagtgccttg ctccgcgcga tatcgcctca ataccaaggc
   129181 ttcccacacc cgcgaaaaga tcgagtacga cacagtcctc aacgcgatcg gcaataatat
   129241 tgaagatcgc ctcgcgcaca cggtcagagg taggtctaat gttgcgcccc ctgatcgtag
   129301 ccaaccggcg acctcctgca atgcctccaa taacacgcat cttacttgac tatgctattg
   129361 ggccgagcct cagctgcagc tcccgacccc aatacgttct cgacaatgac cagcaccgtg
   129421 aatcccacgc ctcgacaagc ctacgaatgg acctaggcgc cttgagcaac tcgaagctct
   129481 ctgattccac cgtatcaatg cagctttcgc agccacgatc gtccaacatg gtgttggagg
   129541 aagcatcgca actgttgtcc gtcattgcag caagccgaga catggctcat ccccctttca
   129601 ctcaatagag ataaggtaat agtaaagcgg ttgaccgccg tattgcactt cgaactcgat
   129661 gtcagggtat tcctctgcca agctatccgc gagcgactcc gcttcttcct cagtaacact
   129721 gaacccgtag atgatagtgg cgatttcgtg ttcttcagag tacatttccg tgaaaacctt
   129781 gcggacgact tcgtccacgt cttcgccaac aacctgaatg tcaccgtcaa ctatgccaat
   129841 tatgtcgttt tcgcggatct ctattccatt gaacgtagag tcgcgcactg catacgtgat
   129901 ctcggcgctc actaccttgc cgatagcctc gttcatcttc gccacattgt cttccacaga
   129961 agtatacggc gtcatggcta agagcgcagc taagccttgg ggtattgtct tgctcggcac
   130021 cacgtgtatt ctcgcttcga tatcaggcac ctcggcagct tgtcgggcag tgagaaccac
   130081 attcccgtta ttaggaagaa tgatgacgtc tttggcatgg gcttcgcgta tcccatcaac
   130141 gatatcctga atgcttgggt tcattgtctg cccaccggtt atcacaacat cagcgccaag
   130201 gctctgcagt atcttctcta caccatcccc ggccgccacg gcaacgatgc ccacatcctt
   130261 cagctcgtag ggctcttttt cgctggaatg cgggaacact ccgttttccg caccgctcat
   130321 ttcctcgaac tcttcgtgct ggtcctccat gttatctatg tctatttcgt gaagcgagcc
   130381 atacccaatg ccgatctcga gaatccgtcc aggcacattg ctgtgcatat gcaccttaac
   130441 tgtgttttca ttcccgacga ccaacatgga atctccgtca atttcggcaa gaagccggct
   130501 ctttatcttc tcaggatcga ggccgtttcc acgaattatg aactcagtac agtaggcata
   130561 cttgatctct tgcttcacag ccataggctc tctggcaact ttcaacgccg tctcagcttc
   130621 agcatgcacc aaggcagcag gctctaccgg ctctcccacg aggcccttca taaacccttg
   130681 gacgaatata acaagcccct tgcctccggc atcaacaacg cctgcttcct tcagtgcggg
   130741 caacatctca ggcgtcttct cgagcgcagc ttcgccctgg cgcaatgcct cgtcgatcac
   130801 tccaaggatg tcagcgccgt tcttggctgc ttgaactcca ccgcgggcgc ctgtccgcgc
   130861 aacagtcaga atcgtgccct ccactggctt catgactgct ttgtaagcaa cactggaagc
   130921 ttcctggaaa gccgctgcca aatctgtcgg attggccgac gcaaggccat gaaggcgttt
   130981 ggcgaatccc cgaagcaatt gagagagtat gacccccgag ttgcctctgg cccccatgag
   131041 agaacctaaa gctatggcat cagccacgtc tcccactgat gccgaacctg ccttctccgc
   131101 ttcctttagc gcagcagaga ctgtaaggta catattagtt ccggtatcgc catctgggac
   131161 cggaaatacg ttcaatgagt cgaccaggct cttattcaca ccaagccatg ccacgccaga
   131221 ctcgaaggca gcaacaaggg ccgccccatc aatcgtcttc atctgcgagg ccggactctg
   131281 cttcgttcca gacattattt ctgccgttcc tttccgcctg cacccaccct gacaccatga
   131341 acagtaacgt tgacttcagg tacattgagg cctgtggtag tctccagaat gtactttact
   131401 ttatccatta cattgttggc cacctcagtg atacgggtgc cgtattctac aataatataa
   131461 agatcaatcg ttgcattgtc gccttgcaca tcgacctcaa ctcctcgggc tatgttgtcc
   131521 atccccagga gttctgcaat tccatcagta aaagtgcgag acgccattcc cactaaccca
   131581 tagcactcgg ttgcagccat acccgctatg gcggctataa cctcgtctgc tatggtgatc
   131641 tggccaagtt ctgtacttat ttcttttccc atcatgaaac ccccaatgct cacctgcaac
   131701 ctacgaatcg agctccacac gagtgaacat tttttctgta ttcttcgcgc ccactggata
   131761 ttccttctca gacccgctac aatcctcttg cgcagcccct ctgcgtatgc tatcatttag
   131821 tttgtgcgtt tcaggaactt gaggaggaat tattatggcc agagtatgcg atatatgtgg
   131881 caagggcccc gtttcaggaa actcccgcag ccactcggag atcaagacca agcgcagatg
   131941 gtatcctaat ctccatcaag ttagggcaga gattgatggc agcactcgcc ggataaaagt
   132001 ctgcaccagg tgcctcaggt cgggaaaggt aacccgggcc atctagcccc aacccctaga
   132061 ttatcaacaa aagcgcaatc atttgacgct ccccttacag gggggcgttt ttcatatgtt
   132121 tcccatcata gtggtagaca gcatggattg aatccatgct gtcgttcatg tcctcttctc
   132181 gcagatcata gcttgcgtac gcctggcacg ctcataaagt ctcagcacga cctcgtaatc
   132241 ggttgttgcg gatccgagaa caacccccga ctgtgagtag ggtccatggc aatctgaccc
   132301 cgcagtaacc aacaacccat attcacgcgc tattctctgg taatgttcct gctgccccga
   132361 agtgtgctgg ggatagatcg cttcgattcc ctcaagcccg tgttccacca aagaaggtat
   132421 catatcgtct tttcctgaaa ggcctggatg agccagaaca ggcactcctt tcgcagccag
   132481 aacggcctct atcgcctcat agggagtcaa tttggacctg ctttcatagg ctggcgcatg
   132541 aaggcctatg taatgcgcaa acgcctcatc ccaactgagc acgtagcccg ctttgaccat
   132601 tgcagccgct atatggggcc tgccgactgc gccatcaccc gcatgctcta acacgtcttc
   132661 catgctcaga tcgattccga gagccgcaag ctttctgagc atccgctcca cacgccgaac
   132721 cctgctctct cgttgtgctt ccaggaattt cagcaaatcc tcgtcttcgt agtctatgaa
   132781 aaaccccagt atgtggatct cgccatcact gacatcagtg gatatctcca cgccgggcac
   132841 aacatcaacg caattctcct caaccgcctc aagcgcttcc cctatgccgt ccacacaatc
   132901 atggtctgtt atggcaacgg cggcgaggcc gcggcgggcg gcctcgctaa ccaactcagt
   132961 gggcgtgaac gtaccgtctg actttgtagt atgacagtgc agatcaactc gcccttctaa
   133021 cacctacagc caccttctcg ccattgacgt cccattcacg cacgtactcc ccttcaggct
   133081 cgccatgggc aagcgaaacc gagagcgtct ccttcatgag ataatccctg tgaacctcca
   133141 gcgcgcgcat ggagtcgggc ccacccgaga tcactgtgca aatgcggtca gacacgtcaa
   133201 atcccgcctc cttgcgggtc gtctggatct tgtttactag ctctcttgcg aacccttcca
   133261 gcttgagctc ctcagtgatg actgtgtcga gggctatcgc tcgtccgccc tcggactgta
   133321 cgtagtatcc ctcggcagca acagtttgaa tggacacttc ttcaggaagg atggatacct
   133381 cagtcccatc gctcaagacc aatccaaact cgcgcccttt tagaaccttt ccagctatct
   133441 gcgaagcatc tgcttcggcg atgccttcgg caatcgcctt catcaacttg ccatgcttcg
   133501 gtcccagaag atcatatctc ggtttcactc tatacgaaag aaactcgctc atatcctgag
   133561 catactccag tctctttatg ttcagctcgt ccttgacaag atcttcaacc aacctgagat
   133621 cttttttctc ctctggagtg acacctacaa ctactttcaa caggggctgc ctgttcttga
   133681 tattcacctc gttgcgagcc gccctgccca agaacacaac ctgcctggcc agatccatct
   133741 gtctttccag atcgacatcg cgcgcccatt cgtctgcttt aggatagtcc tgcatgtgta
   133801 cgcttcctga gccaggttct agattggtga atatttcctc cgccaagaaa ggcgtgaacg
   133861 gcgcaatcag cttggcgatg gttgtcagag tctcgtggag cgtgagatat gcggcttgct
   133921 tgtcgatggt catctcgctt gcccaatatc gacgacgaca tctgcgcaca taccaattgg
   133981 atagatcgtc cgcgaactgc tcgattcgcc tcgcggccgc tgtgatgtca tagcagtcca
   134041 tcgcctctgt aacttcgccc acaagcccgg atagcttcga caggagccac ctatctatca
   134101 cagagcgatc ctcgggggca acatggtatt ggctaggatc aaattcatcg atgttcgcgt
   134161 atagcacgta gaacgcgtat acgttccata gcgtccccat gaatttgcgt ttggcctcga
   134221 taactccatc ctcgtagaat ctcgtcgggt tccatggcgc gttcactgtg tacagatacc
   134281 accttacggc atctgctcca tgatcatcaa agagcttcca tacatccacc acattgcctt
   134341 tgctcttgct catcttgagc cctgcctcat ccagaacaag cccgagcacg acgcagttct
   134401 cgaagggagg cctgtcaaac aggagcgtgg agatggacaa gagcgtgtag aaccatccgc
   134461 gggtctggtc caccgcttcg gttatgaaat ccgccggaaa ggctgcagag aaagcttgag
   134521 cattttcaaa cgggtagtgc cattgcgcaa acggcataga gccagaatca aaccaacagt
   134581 ccattacctc aggcacacgt ttggaatctc ctccacactc ggggcatgcc aaaactgcct
   134641 ggtcgatgta cggtctgtgc agctctaaac ggtcgggcaa cgatttcgcc atgctcttca
   134701 gctcttcaac actgccaacg gcgtgttcat ggccacactc ctcacatacc cagatcggca
   134761 acggcgttcc ccaatagcgc tcacggctga ccgcccaatc aatggcgttt tcaaggaagt
   134821 tgccgaatct gccatcccgt atatactcgg gataccaact gatcttggcg ttattagcca
   134881 atagctgctc gcgcaggtca gacatgcgta taaaccaaga gccccgcgcg taatagagca
   134941 gcggcgtatc acatcgccag caataggggt agctatgtgt atagtccatc tcttcaaaca
   135001 aaaggccgcg cgccttaaga tcttgcacaa tcagaggatc cgcatctttt acgaacatcc
   135061 cgctccaatc acgagccctg tcatcaaatc tgccttggga atccactagc tgtattatgg
   135121 gcaagccatt gttctgtgca acgcgcatgt cgtcttcgcc gaacgcaggt gctatgtgga
   135181 caattcccgt gccctctgaa agagtgacga aatcgccctc tacaaccatg taggcaggcg
   135241 cttcaggatt ctcatacgta taaagaggca cgtattccat gcccacaagc tcggacccct
   135301 tgaccacccg cacgattctg taatcgcctt tgaccctcga ctcgaccaaa tcctttgcga
   135361 gtatgagcgt ttccccatcc ctctcgacat aggcataatc atagtcagcc gacacagcca
   135421 atgccacgtt agacggcaaa gtccacggcg tagtcgtcca tgccagaagc gatacattct
   135481 cctcgccttt gaccttgaac ctcacgaaaa tggatgtctc ttcaacctct ttgtatcctt
   135541 gcgccacttc atggctggat agcgcagtgc cgcatcgggg acaatagggg accaccttgt
   135601 atccgtggta catgaggccc ttgtcccaga tagtcttcac agcccaccag acagattcga
   135661 tgtactgatc atggaaagtc acataagggt cgtccatgtc tatccaaaag cctagccttt
   135721 cagtcatgcg acgccattca ctttcgtatt tgaagaccga ctctttgcac cgctggacga
   135781 actcctctac cccatactcc tcaatctggg gcttgccact tatgcctaat tccttctcga
   135841 cttcgagctc aacaggcaaa ccatgagtat cccagcctgc cttgcgtggc acgtagaagc
   135901 ctttcatggt tttgtagcga agtacgacat ctttcatggc gcgcgtaaga acatgtcctg
   135961 gatggggcag accatttgct gttgggggcc cctcgtagaa gacgaaccga gggccttttt
   136021 ccctccggtc gacgctcttc tcgaatatct tttcttcccg ccaaacctcc agtatctctt
   136081 cttccattcg agggaagtcc atagccgaat cgactttctt gaacattcgc agacataacc
   136141 tccttgtctt ctgtgtcacg gacatgtagc agcccaaaac agaaaagata aagccccccg
   136201 tccaagggac gaggggttaa ctcgcggtac caccctaaat tagccgacac atggcctggt
   136261 ttcaccaggc ggcaacggaa ccgtcgcaga aatgtcggcc ctctcgttag ccccgataac
   136321 gcagggatgc ggcacagctt actcgttccc tttcagcctg caactcgggg gcgattttcc
   136381 cgacccgacc agcatggaga tcccaccatc ctccactctc tttggccgtc ccgatcgata
   136441 ctcttccccg tcatcgtatt tcgcctattc aactaggatc aatctaccac acagctgcat
   136501 acagcgtcaa ctcatggccg attcttgacg ggaaaacagg gttgccccgt ctgcgaccct
   136561 ccctgtctcc tc
//
LOCUS       DAOSYL010000003        98982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_149_length_98982_cov_7.338593, whole genome shotgun sequence.
ACCESSION   DAOSYL010000003 DAOSYL010000000
VERSION     DAOSYL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 98982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 98982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98982
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149_length_98982_cov_7.338593"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(736..>1483)
                     /locus_tag="PLU15_01290"
     CDS             complement(736..>1483)
                     /locus_tag="PLU15_01290"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO29588.1"
                     /translation="SLIILAMAFTGAGCTWFGPKVVVNGRVIATDANGKEIPIYRASV
                     TIGKVSTAVTDYDGRFQISGVKAGRQTVTVQKEGFQTLTKAVEVKADGSDIGDLEIVP
                     EEGTETIMIEPKWTLLPIGSEAIAELEAKGVLDDIPEGTVIAEATIDVTLEQDGGKRY
                     TAGARMSLERTASGVEVVDEGALIIMGIQYQAGAGYTFAVTGKVGSDLDRFKQKNIAV
                     SGSGRIEAIEFEGGKSYGVTISLHGFLEDR"
     assembly_gap    1484..1583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1612..2067)
                     /locus_tag="PLU15_01295"
     CDS             complement(1612..2067)
                     /locus_tag="PLU15_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO29589.1"
                     /translation="MAYCPACGAGCNPDDKFCKECGARLKILDSSEPERHMTAQEEVL
                     WEGKPYLKEIARNTNYVVTNQRILITTGLLGRKEEQIDLARVKDIRMTQGLSDRIMGI
                     GQIEIISTDPSTPEFRLVGVKDPQRVRDLIWEATNRRRKEMGIRHREIL"
     gene            complement(2627..4102)
                     /locus_tag="PLU15_01300"
     CDS             complement(2627..4102)
                     /locus_tag="PLU15_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO29590.1"
                     /translation="MEKPMDRNYKLFIDGKWVDGKEGKTFDTYCPANGELLSTCVEAG
                     PEDVDLAVKAAWKAYDSWKLTSPAQRSQILLEIADRIDANTEKLAMIETLDNGKPIRE
                     SATLDVPLGADHFRYFAGVARAEEDSAVMIDDQTLSIILREPIGVVGQIIPWNFPFLM
                     AAWKIAPALAAGDTIVIKPSSATPLSILEFAKIIQDVVPPGVVNVVTGRGSTTGQYML
                     EHPGFRKLAFTGSTEVGYNIARAAADKIIPATLELGGKSANIFFPDAPWEKAIEGVQI
                     GILFNQGQVCCAGSRVFVHEDIYDKFLAECVEKFNSIKVGLPWKKETVMGCQINEGQL
                     QKILKYIEIGKDEGAKVVCGGTRIEEGELARGPFMRPTILADVTNDMRVAQEEIFGPV
                     VCFIKFKDEDEVIKMANDSEYGLGGAVWTRDINRALRVARGVETGRMWINNYNNIPAH
                     APFGGYKKSGIGRETHRMMLDHYTQKKNILIHISEAKMGMY"
     gene            4415..6424
                     /locus_tag="PLU15_01305"
     CDS             4415..6424
                     /locus_tag="PLU15_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOO29591.1"
                     /translation="MASEYIPHVVAKAWEDLVNTGTVRVEALRPVVASSWTRSQQAGI
                     DPRDAVGKIVLSQAQLENVILDRSDLIEVARPFMSSLYEFVAGSGFVVIISDELGRVM
                     EVMGDENALRRGALVNLLPGSSWAEHHVGTNGIGTALAIAKPVQLSGEEHYCEKFHPW
                     TCSAAPIRNSNGSIIGALQMSGPSSKTHIHTLGMVVAAIEAIEAQMKINAKVRELTVL
                     NDNLNKMFQIASDGIVLVNNEGIIQRTNPVADRILGTTRPTSFMEATGAPSHLEKMIS
                     NGTAFDDVEMSVETPEGRVQCLVSGKAIYDEDGEIRGGVVSLNPTKRIAKLMGRYSRS
                     YATFTFDDIIGRDGNLRRAIKLGHIAAESDSNVLICGESGTGKEMFAQAIHNESRQAN
                     GPFVPINCGAIPRELVGSELFGYEEGAFTGAARGGRPGKFELASGGTLFLDEIGDMPL
                     EQQIALHRVLQDKMVTRIGGANMTEVDVRIICATNKNLQHEVAKGNFRQDLYYRLNVI
                     YILLPPLRERREDIPLLFDFFLSKAMSQLGVDISNVDPDVTGCLTSYDWPGNVRELQN
                     VVERMVNIATSGYIGLEHIPVEILNPSRSGNTSTQMGSPFPAAKPPTFEQQRAEIRRT
                     AEQRQREELIRALEKHDGNITRTAEEMGVSRNTIYRRMKKLDLQR"
     gene            6766..8337
                     /locus_tag="PLU15_01310"
     CDS             6766..8337
                     /locus_tag="PLU15_01310"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOO29592.1"
                     /translation="MRRKQVNILGFVLSENAGVAVVGIKTGLIMPTDDIVEVTMDAVE
                     DILEDGDIVCVTEAVVARSQNRYITCDELAEDVKAKLNIKDNGTVAVISPIVSRNRFA
                     LVLQAIARAVNKGRVIVQLTVPCDEVGNQVIDEEFANNRLRFKKVLRSLQEVRGNTPQ
                     MNVLIREIIAALKFQELGYSVNAIRKITGTGMADITLLDKDGRKVIAEVAFENLAGVA
                     QKAIGIAKDDKADIAMAVAVDLQAQEIAIVEATSFLEGKAKPEVYKYDERQAFYEATD
                     VITVAEIGERLFPHPITGIDYGRMYAATIESEGAQAEIVFTNNPLAVFNFGHIDGIII
                     GAVHERESLKNLFLSFGTKTPMLTIQDIGPEPWGVIGSNVSDLDKGILKLLPDNSDDV
                     CDKIKAAAWEKTGKDIEVLIFGDGAYKDPDTGIYELADPYPSIGCSKGLRSASLRQGT
                     KLKLLVETMFREGHSREEIYKALEEQQPSRETLGTTPRNITGILATMADLAAGSADAG
                     TPVVIIRNFPFKPAK"
     gene            8626..9027
                     /locus_tag="PLU15_01315"
     CDS             8626..9027
                     /locus_tag="PLU15_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOO29593.1"
                     /translation="MKGFAIDTFEKNQVVDITAQVESCLADQGPQDGICLVQTPHTTC
                     GIILNEHFDSTVSEDILDCLNRIAPEDADYSHKEGNATAHIKAALIGSSVILAIEDRR
                     LALGTWQRILLCEFDGPRQRKVWVRTLKQGT"
     gene            9133..10167
                     /locus_tag="PLU15_01320"
     CDS             9133..10167
                     /locus_tag="PLU15_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO29594.1"
                     /translation="MANFRIPDVRRLPAIASIFVILALIGSVCARPLSAAADSAITVT
                     FIDVGQGDAILVSTDDGRHMLVDGGPKAAGGKLVATLHSMGVEHLDVVVSTHPHEDHV
                     GGLIEVLDSFAVGKVLDSAKAHTSATYEAYLSKIYEKGIAFSAPRAGDSFDLGPVTIK
                     VRWPTEPLPQDINDSSLVLEVAYGKSRILLTGDISGHVERILADTGVLNHIHLLKVAH
                     HGSRTSTTQTFLAQTSPRLAVIQVGQGNDYGHPSNQVLTNLRSRDVLAYRTDEHGSVS
                     VKMRPDGKTFSVHIEQPGNDLRASSSSDVFHRSSCRYVNTIDASRIVMISDREQAMNA
                     GYRPCKLCNP"
     gene            10193..10438
                     /locus_tag="PLU15_01325"
     CDS             10193..10438
                     /locus_tag="PLU15_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HOO29595.1"
                     /translation="MKLVATLDRIENGIAILLVRPEESIQILWPVSALPEDAVEGAIL
                     DISVDVDKGKTQAAEERVQSLLDKLLGKGSQQSTEPI"
     gene            complement(10518..10781)
                     /locus_tag="PLU15_01330"
     CDS             complement(10518..10781)
                     /locus_tag="PLU15_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29596.1"
                     /translation="MEQEHKFSCECGKPYVEIIEDSFETLLEDYVVCSKCGRRIEVEG
                     EFAQALSAALGQLQDGLKKIADDMRPGERSKGGCGCGGHGHIV"
     gene            complement(10935..11720)
                     /locus_tag="PLU15_01335"
     CDS             complement(10935..11720)
                     /locus_tag="PLU15_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOO29597.1"
                     /translation="MYVAGVDVGSTAVKAVVLLDGRIAGTNVISTGTDQEKTAFQSLS
                     AAAQDAGVVDIEDISAMVATGYGRSMVKMADRTVTEITCHARGGRAFAEAAHVIVDIG
                     GQDSKVIRLDEDGMVADFVMNDKCAAGTGRFLEVMAERLEVPLSDFGKMWRATRKPAM
                     ISSTCTVFAESEVVSLLSHGVAREPIIRGLCDAVADRLAGMVHRVGLATEVVMTGGVS
                     RNDGVRLSLERKLGLKVSVPSMSQFAGAYGAAIIALELAEKGR"
     gene            complement(11727..13010)
                     /locus_tag="PLU15_01340"
     CDS             complement(11727..13010)
                     /locus_tag="PLU15_01340"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOO29598.1"
                     /translation="MGPRGTRSVGNSLQVRAFRRFARKPWCYGAAKQVVDIMERRGWK
                     PYQISAIRCVMDDFRQAYTGERPCIYTTAFVPTELVYGLGAVPFLPEVAAGYAAAFGF
                     AYDALGAAESMWYSADLCSFHRAGVGAGKLGVLPRPSAVIATAHLCDGGKKSLHRMSK
                     DAGCPFYLLDVPYDRSERGQKRLRDQVEHIAFDLPEKVPGISPEGMAEAIRVSNATLD
                     DYLEVCALRQHSPSPWRGGEALNYVVMFLGAWGNPWLAEFYKDLASHIRGIIASGEYP
                     CCSERHRLLWLNLRPYYKTGLLDYLELELETSVAFEEYNWPYWSELDPDDPFASVARK
                     MLANFGWGPIERRLAAVDSMIDDYSIDGVVQFAQWGCRQSNGGASMLADHLKRRGIPF
                     LNLGGDGVDVRNGSEAQAMTRLGAFVELIDAKKAR"
     gene            complement(13000..14112)
                     /locus_tag="PLU15_01345"
     CDS             complement(13000..14112)
                     /locus_tag="PLU15_01345"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOO29599.1"
                     /translation="MLLTSCSYIPLELPLAAGLPAKRLFVLDGAEVDADGLLPRDFCP
                     YARALMREYDPGDVLAIAGSCDAMRRAYDAIRYWGLAADVFYLDVPRTADEAAVDYFA
                     GELRQMARHIGVVGPAGQVEEQSLRLACEAMNKARAAMAEAAKSLEEGTLRASRLLQA
                     IMDVNELMGDVARWGMTWNREEGFGREVCDALTRAVRAEIEKALSVSVQPPALAGRVR
                     VGVSGTCLLDLSVVECIEEAGLEPVFIDSCMCQRVYDFSVDVNPSYDIYMELARSYLS
                     KPGCPRMFAGDERIARLGALAAMYGAKGLVYFAPKFCDLGYYDFPDVKRGLRESFGLP
                     SLLLEGEYGAGRTGQVLTRAVAFREMLEGRGEPNGA"
     gene            complement(14242..15099)
                     /locus_tag="PLU15_01350"
     CDS             complement(14242..15099)
                     /locus_tag="PLU15_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29600.1"
                     /translation="MIWDGAFGALLAGMIVAASLKGVTEMLLRTVGPVANYRRKPVAF
                     PGGLVVPFGAVLGPILVGALGGIHVDEPGRVGAYLLVIVGFGFAGFIDDMLGGHAQSG
                     FSGHFGALSQGSFTTGVVKVFLGGAVSLGAVELLAMAPVGIRDLMDAAVVALSANLIN
                     LLDRRPGRAIKGFLLGFAALSMWFWMKDGKAGLARMLSPAGSALGAAIAFFPADLGET
                     AMLGDTGSNALGAALGYCLLQMTVGWRTSALIVLVWLTLASERMSFGKVIEDNRVLRF
                     IDRLGREKE"
     gene            complement(15552..16250)
                     /locus_tag="PLU15_01355"
     CDS             complement(15552..16250)
                     /locus_tag="PLU15_01355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO29601.1"
                     /translation="MHPRVAIVPALNEEPSVGQSVAALLQCSAADCVIVVDDGSRDRT
                     SARAKEAGATVIRMAKTVGKGAAVATGIARARKMAPALLVFADADLGPSAAEMASVVS
                     AVVEGRGDMAVAGFPQPISSGGFGLVVGLAKRGVRQLAGLDLKWPLSGQRALLAEPFY
                     EVFAGDGAHMPRLDRGFGLEVGLAIDWARAGYTIVEVPTNMTHRETGKDLAGICHRAR
                     QFVEVLEALTMRRR"
     gene            complement(16250..17062)
                     /locus_tag="PLU15_01360"
     CDS             complement(16250..17062)
                     /locus_tag="PLU15_01360"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HOO29602.1"
                     /translation="MSLRYHVVSLAAVLVALGIGILLGAAISGGEGLAAQQRTLIDRL
                     DADFYRLSQDRDALAQELDAARRYAEESVPYMIRGSLVGKTVGIVSMPDVDRGDLESA
                     RDVCEGAGARVGAVVELADVALAGANSGQLSVWAQAITSSDMDRIKSLSTQGLAKIRM
                     AERCQFSVVLALSGPAEAKEAAFLQALSAAAKSAGTPLVVGWTRAAVLKVRSDVAAGK
                     FSSQLGKALPAAEVYGVGTAPGNVAMALALAGARGVYGQPPAPAALPAQEGR"
     gene            complement(17096..18286)
                     /gene="steA"
                     /locus_tag="PLU15_01365"
     CDS             complement(17096..18286)
                     /gene="steA"
                     /locus_tag="PLU15_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOO29603.1"
                     /translation="MGYDGVDGKQSIGAEDSAGNARVRIRGTARADRKTKNLISRLRP
                     GEIAVIAHADIDEVSGRALVEACPKMVINADRSITGRYRCLGPGILLDAGIPVLDGVG
                     PHLLDVVADGDALEIEGNEVFKEGQPIGVGVWVTRDSLEAAYIEAEENLDAELEKFVV
                     NTLQYAIKEKDIILGDIDIPPLETQIHGRHCLIAVRGPRYREDLDAVASYIREMAPVL
                     IGVDGGADALVERGFAPDIIIGDMDSVSDETLFLGAELVAHSYPDGRSPGAERLANLG
                     LSHKTFVCPGTSEDIAMLLAYEKGASLIVAVGTHSNMLDFLEKGRPGMGSTFLVRLKV
                     GSVLVDAKGVSQLYRGRLRIGHIIGILLAAVVPLAAIAFFSGSVADTARLIYLKIRVM
                     LGLL"
     gene            complement(18350..19159)
                     /gene="spo0A"
                     /locus_tag="PLU15_01370"
     CDS             complement(18350..19159)
                     /gene="spo0A"
                     /locus_tag="PLU15_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOO29604.1"
                     /translation="MSQGLVRLVMVDDNVDLVEVVTDFVSTQPDMSMCGVAFNGSEAI
                     SVIRDVAPDVILLDLVMPHLDGLGVIEYVNSTPLSPHPRILVITAMGLETTAAGVVAA
                     GADYYMAKPFDLEVLARRIRRLAALPPERPQELQSCASCPQSTDLQRSIYDVEVSKTL
                     HRMGVPANLKGYLYLKDAIIMALERGHGAGSMTYSVYPTIAEAHGTTPSRVERAMRHA
                     IETAWNRGDIDFINRIFGHTVDAEKGRPTNSAFIARVASKVRIDLAGQIIR"
     gene            complement(19224..19481)
                     /locus_tag="PLU15_01375"
     CDS             complement(19224..19481)
                     /locus_tag="PLU15_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO29605.1"
                     /translation="MPQDRPELDSSAALALEKLSPREREIIGMVALGLSNQAIGEKLF
                     ISDKTVKNYVTSIRRKLGVENRIQVALYAIRCGLVDPAIDQ"
     gene            complement(19630..20946)
                     /gene="spoIVB"
                     /locus_tag="PLU15_01380"
     CDS             complement(19630..20946)
                     /gene="spoIVB"
                     /locus_tag="PLU15_01380"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOO29606.1"
                     /translation="MRAYSSKRIAIAFAVIVGFLSLAAIGAAVARIYVSPIYIRVFEG
                     QTFSVDMGPLGRGAVVVDPPDSLLMDAAIGSKVEVTPTKAGRTRLEVRLFNAIPVKRL
                     VVDVVPQMRVIPGGHSIGILLASRGVRVMGMYPVVGSDGLRYMPARDGGIEVGDMIVS
                     VDGYEVHEPRQLETLVSEGALGGRPVRMGIVRDGREFDVTVLPAPADGGYRIGVYVRD
                     NAAGVGTLTFWHPGTMRYGALGHVVVDSQTGKEIPITDGRIVSARVSGIDLSGAGRPG
                     EKIGTFAGARDNVGVVEANTEVGIYGTLDAPLPNPYYADPIPVALAAAVHEGPAEILT
                     VVTGSSIERFEIEIVKVAKQSRIAGKGIVIRVVDPRLLELTGGIVQGMSGSPIIQDGK
                     LVGAVTHVFVSDPSRGYGVFAEHMLNVAGLMEGSERAAGWIVLLAA"
     gene            complement(21250..22740)
                     /locus_tag="PLU15_01385"
     CDS             complement(21250..22740)
                     /locus_tag="PLU15_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOO29607.1"
                     /translation="MTRRPKFAASKTARDLAVEVAYLCDQEGMYANAAWSQALSGKEM
                     DARDRRLAMEIAYGSIRRKKTLDWWIGHASTRPVDLMDPLARALLRTGVYQLLFMSRV
                     PPHAAVSESVESAARLGHRGLASFVNAVMRKITSAEFSPEFPDPKGDPVGYLSVSLSY
                     PEWLVQSWIGEYGVDVARAMLEAGNEAPTIVLRVNRLKIEREEFSRMLKSEGVLSEPG
                     HLCPEALILSSPIDGVEELPGYHDGLFSVQDESSMLVGHVIGVAELVCAANGKKPGAL
                     WAESGKEDESGVAEEAIEDGHWPLIIDACAAPGGKAAHMAELAGCGAKVVACDVALER
                     VKMIEDSVARLGLPSVEAICCDARELPDIYSGKADAVLVDAPCSGLGVLGRRPDARWR
                     RSPDDILELSELQEEILDAAARCVKPGGVLVYSTCTVGTRENRDQVQRFAVRHREFVP
                     SPIAPYMPSAAARAFCSDDGHMIELLPGISGTDGFFISRFYRKRDI"
     gene            complement(22733..23437)
                     /locus_tag="PLU15_01390"
     CDS             complement(22733..23437)
                     /locus_tag="PLU15_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOO29608.1"
                     /translation="MPFYFYYDPTYIIVIPALLLAIYAQAKVQGTFNRYMRVRSKTGF
                     TGSQVAQSILRSNGIHDVGVEPINTPLGDHFDPRSKKVRLSPAVYRGSSIAALAVAAH
                     ESGHAMQDKQGYAPLAFRQAILPVANFGSHMAFPLFILGMLMGYDKTGQFLMSLGIWL
                     FAGAVLFQLVTLPVEFNASKRALTLLADGGYITQDELPAARQVLQAAALTYVAASATA
                     ITQLLRLLILRGSQRD"
     gene            complement(23476..24459)
                     /gene="fmt"
                     /locus_tag="PLU15_01395"
     CDS             complement(23476..24459)
                     /gene="fmt"
                     /locus_tag="PLU15_01395"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOO29609.1"
                     /translation="MRIVFMGTPEFAVPALRALTDAGHEIICVVTQPDRPRGRGYKLA
                     SPPVKVEAERLGLPVVQPARVSDPEFVEGFAEMAPDVAVLAAFGQKISAELLSVPRHG
                     FINIHPSLLPKYRGAAPIERAMINGDQVTGVTIMYMDEGWDTGDIGICQDMPIPSDAD
                     RGWLECALAELGGELIVKFLRLIECGEAPRVPQDHSAATYARKLSSDEFEIDWRKSAR
                     EIGNLIRALSPAPGARTSFAGQSVRILRAHEIAPVLVGDGEAGETVCSGYPGMLTIGP
                     EGELIVFCGLNGCELMALDVVHPAGKRQMPAADFARGRRISHGDRFGSGMC"
     gene            complement(24463..24954)
                     /gene="def"
                     /locus_tag="PLU15_01400"
     CDS             complement(24463..24954)
                     /gene="def"
                     /locus_tag="PLU15_01400"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOO29610.1"
                     /translation="MLILEIRTEGDPVLRETAKPVKIIDGKMRDLARDMLETMYSAPG
                     VGLAAPQVGIPVRLIVIDAGDGPRTLFNPEIVWRRGEAVDDEGCLSVPGMIGTVTRSA
                     EVEVIAYDRDGKLVSVRGKGFLARILQHEIDHLDGVLYIDKAENVRPVEEECEESQCS
                     EGD"
     gene            complement(24975..27443)
                     /gene="priA"
                     /locus_tag="PLU15_01405"
     CDS             complement(24975..27443)
                     /gene="priA"
                     /locus_tag="PLU15_01405"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOO29611.1"
                     /translation="MDGRYAQVVVDVPARRVDKCYDYIIPDNLMDKIVPGSRVLVPFG
                     PRSVEGFVVARTEKPAVAKVKPITACVKSAPPVPEELLAVARWMADEYMCLLIEALRA
                     LVPSGTKLESSTIVRVCATDEDAEGAKISGAAKEVFDYLVECGGSARRDEIASSLEIA
                     DITRPISALTKAGLVETDTMWAPPRVRPRTVRAFRLASEEIDPQALALSLAPGSPARA
                     AVMRVFAEDIDKTGTKGLAIPRAELLERAGCSSSVVDALVRDGMLERMDVAIQRDPTA
                     GLQIEKSVPPVLTDDQEHAVNRITDVAENGGGVVLLHGVTGSGKTEVYIRAIQHVIDR
                     GKQGILLVPDISLTPQMVDVVMGRFGDRVALLHSALGAGERLDEWERARAGDADVVVG
                     ARSAVFAPVPSLGMIIVDEEHDSSYKQDDSPRYNARDVAIFRAKQVGATVVLGSATPA
                     LETYELSELGECERIELPSRIDHRPLPEIRVVDMRREMKSGNKSVFSNLLRSSVEEAL
                     DSGQQVMLFLNRRGFSTFAMCLECGQAISCPNCDVSLTYHARASEMVCHYCNWSQPVP
                     DVCPVCGGMNMKYSGTGTERIEEAVRKEFPRARVARMDVDTTRRKGAHRAILGAFRAG
                     RTDILIGTQMIAKGLDFPNVTLVGVISADTSLNLPDFRAAERTFQLISQVAGRSGRGS
                     EPGLVVVQTFNPEHYSIMAAAAHDYRAFYDAEIAARREMGYPPARRMARILVRGEDES
                     ATAKAAGKIRRACTGTEPVARGAVEVIGPAPAPIQRIAGKYRWHLLLFSSDADAIRAV
                     AAAGCQPRLAGVTISVDMDPSSIL"
     gene            complement(27452..27988)
                     /locus_tag="PLU15_01410"
     CDS             complement(27452..27988)
                     /locus_tag="PLU15_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOO29612.1"
                     /translation="MRFCHGIMGYGERRNHDLLKPDLRVSGVWEIDLDDLLARGISGI
                     ILDIDNTLAPWGSYDIPGPVREWIEEAKARGFHLCILTNNYGARADWFSRELDVGAVR
                     GWVKPWAWGYRRAMRKLGTDADSTVMVGDQLFTDIRGANRLGIYSVLVLPLSDRELPH
                     TRFKRTLERKILGDIDER"
     gene            complement(28195..28896)
                     /gene="sigK"
                     /locus_tag="PLU15_01415"
     CDS             complement(28195..28896)
                     /gene="sigK"
                     /locus_tag="PLU15_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOO29613.1"
                     /translation="MSSALVAFADLLTRGLMLLHSYVAGNGAFPPPLTEDEERQMLER
                     KEAGDEEARNILVERNLRLVAYVVKKYDVVGEDTDDLISIGAIGLIKAINTFDRQKKT
                     KLATYAARCIENEILMHLRSGRKNRGELSLYDPIGADKEGNEIILMDVLTNGGDTVAD
                     IVESKVEQERLVEKLAKLSPRERRVLELRFGMGEGRRQTQREISRALGISRSYVSRIE
                     KRALAKLMKEMSTPS"
     gene            complement(29003..30571)
                     /locus_tag="PLU15_01420"
     CDS             complement(29003..30571)
                     /locus_tag="PLU15_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO29614.1"
                     /translation="MAAKAVEQRSVDAVIRLARGPIFDRNMELLAGGTSIGLGGDLGE
                     DGAALAVAIFPQSISGPGDSLDKLERMIPGVLDGAPRVIRLPGYDRKEIVGMAKAVTG
                     IVPISIPSRWSGLASHVVGYLTGSQVGAEGIELAFERFLGGGGQGRDVVRAMCDSRGK
                     EIPGLGVRLAVAGEEPGWVRLSIDRRVQEAVERAFDRRAPGLAGAVVVMDPWTGQVMA
                     MASRPSYDQNDVSSSLARADAPLLNRAVYPYYPGSIFKVVVAAAALNEGARMPGSVYH
                     DSGAIQAGGHTFRCHAFERGGHGDITFADAMAYSCNTAFVDAALAVGPGPIVDAARAL
                     GIGSYEVAASTCLPNVSTGQTPPRQARMAAAELANFALGQSVVKVTPLEVATMMCAVA
                     NDGILRHPTLVLEAVHSASGRVMKMDWEAARALDSGVASKLRSMLSGVVSYGTAQDAL
                     GGLNAAGKTGTAQTGRVTETGQPIYNAWFAGFAPADHPKVVISVLVEGAESGADLAAP
                     IFGDICDEILGFFG"
     gene            complement(30865..31488)
                     /gene="udk"
                     /locus_tag="PLU15_01425"
     CDS             complement(30865..31488)
                     /gene="udk"
                     /locus_tag="PLU15_01425"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOO29615.1"
                     /translation="MSDEVLVVGIAGGSGSGKTTVVRSVSQAFGDAVAVLEHDAYYRD
                     QSHLSFEERLKTNYDHPFAFDTDLYISHIETLIEGKPVEKPVYSFESYTRTPQTERVD
                     PAPVIVLEGILILEDPRLRALMDIKAYVDADPDVRFIRRLMRDIKERGRTVDSVVEQY
                     LDVVRPMHLQFVEPNKRYADFIIPEGGFNQVAIELLIARLKSAISSD"
     gene            complement(31522..32580)
                     /gene="mltG"
                     /locus_tag="PLU15_01430"
     CDS             complement(31522..32580)
                     /gene="mltG"
                     /locus_tag="PLU15_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOO29616.1"
                     /translation="MPGTVQTRERARRRLAWPLVFLMALLVTAGAWAIWASRGVSPEE
                     GAEVRVRVVLPDGASASKIAAILKEDGLIRSSVMFDAMARFSGKDSSLKAGEYEFIQG
                     ESVASILDAIASGRVVTVRVTIPEGLTALQIADIFHERGLADREELLALVNNPPHDLA
                     EEFPFVADQSSLEGYLFPDTYEFANGVGALRIVRTMLRRFQHTAQAAFDHHPSRPMSL
                     TDRETLILASIVEREAKHPEERPIIARVFLNRLQKQMKLQSCATVQYALPVPKEHLLN
                     PDLEIDSPYNTYINMGLPPGPICNPGLASIEAVLNPEPGDYLYFVAASDGSHVFTKTY
                     SEHLRAKARIERDSRSSR"
     gene            complement(32910..33227)
                     /locus_tag="PLU15_01435"
     CDS             complement(32910..33227)
                     /locus_tag="PLU15_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOO29617.1"
                     /translation="MPDEVSIVTLVDENGEEQDFAIDDIVEVDETRYAILVPAECLDE
                     LEDEDVCEEAFIFRIEVDDDGEEYFTDIEDDEEYERVLEALEAFDDDFLDDDYGEDDD
                     EDM"
     gene            complement(33378..33797)
                     /gene="ruvX"
                     /locus_tag="PLU15_01440"
     CDS             complement(33378..33797)
                     /gene="ruvX"
                     /locus_tag="PLU15_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOO29618.1"
                     /translation="MVRIGIDLGESRIGVAVSDELGIAAMPHTVIESRGWEQDIIRIG
                     ELAKKVGACEIVVGMPRNMDGSYGPAAQRVEDFCERLERAVKLPVVTWDERLSTVEAS
                     RLLIEADLSRRRRRQTVDKTAAAIILQGYLDWLGIED"
     gene            complement(33809..33865)
                     /locus_tag="PLU15_01445"
     CDS             complement(33809..33865)
                     /locus_tag="PLU15_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29619.1"
                     /translation="MLCGYCVAHCKEFCIKVV"
     gene            complement(34372..34683)
                     /locus_tag="PLU15_01450"
     CDS             complement(34372..34683)
                     /locus_tag="PLU15_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO29620.1"
                     /translation="MQANISPDVGAALICEAVERGINFFDTAELYGVYEYLRRVLHRV
                     PREAIVISSRCYQYTYEGMRESLERALRDIGVDYIDIFGLHEQESRLTLNGHVDAIRY
                     L"
     gene            complement(34762..37065)
                     /locus_tag="PLU15_01455"
     CDS             complement(34762..37065)
                     /locus_tag="PLU15_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29621.1"
                     /translation="MSNHALASAIAAPNGERFEDFPGRRGVIVIIADGFSLRDALEHG
                     GPWLLSFLDSASIGMSNCRTADGGDRLLSAAATFGAGRRMSARESAGYGLGRDERING
                     ERAEDVYLRRTGAEAKRFSGEILCLGYPELEAMGKQSPYRGRPGLIGESLRLAGFAAA
                     AVGNSDVAGVQVRPGVLLVMDARGRVACGAVGAEIVEADPSAPFGISCDALAMAHATA
                     DALFRPNIAAVTVDFGDMNRLGRYLSNLSSEARVEQLGKCYGKLDEIFRALFEAVGPP
                     SDDLAYILFSPGEPSSLGPVAIAGWQYPRGMLSSDTTRREGLVANLDLAATILDGLGV
                     QEAWPHDGSPMIGIRAGEEQNVAERLMRMAESLQIAASSRVPVLSVFIGTVVIAVFSV
                     LAVALLGLRVPAVFVFFAKFLLLSVAAQPLLILVAPILRIQGIAGTLLFASLGSAIIS
                     LLLIRTTSSCMWASVGIAAASAVVIIADGLAGGTLAATSLLGHSAIVGARYYGIGNEL
                     MGVLAGSAAICLSALGEMWTGAERVVSRSGKATAGAAIGVGAISMLLGHPTIGANFGG
                     MVTAAAIAVFMGVMAFARSTLSRRAKVVAMVAIVVVAVGACAGMAWADAKGKRSSHIG
                     RAWRLLTDQTGADGVDADVGAVPEREDAHGATEAIDMGLGQLGVIAWRKLEMNMRLLR
                     YTAWTRVLLAFLIALTVLAGKPFGLFRRFRDDYPIVYVSVMGGLAGALVAMAVNDSGV
                     VACATVAMMPAFALLGYVVDEILAEGA"
     gene            complement(37151..37414)
                     /locus_tag="PLU15_01460"
     CDS             complement(37151..37414)
                     /locus_tag="PLU15_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOO29622.1"
                     /translation="MSGKWDETVKYPAVEAEQEVTPVKKALTEVTIALEEKGYNPVNQ
                     LVGYLLSGDPTYITSHRNARNTIRKVERDEIIEELVRSYLRKE"
     gene            complement(37452..40091)
                     /gene="alaS"
                     /locus_tag="PLU15_01465"
     CDS             complement(37452..40091)
                     /gene="alaS"
                     /locus_tag="PLU15_01465"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOO29623.1"
                     /translation="MKTDELRQAYLRFFESKGHKILPSAPLVPAGDPTLLLTAAGMVP
                     FKPYFLGTAVPEHTRVTTCQKCVRTGDIDNVGKTTRHGTFFEMLGNFSFGDYFKAEAI
                     PWAWEFVTDVLGLAPENLWVTIYLDDDEAFNIWRDVVGVSADRIIRKGKKDNFWEIGV
                     GPCGPCSEIYIDRGADVGCGSPECGLDCDCGRFIELWNLVFIQYHKDEDGNYTPLERP
                     GIDTGMGLERVAAVLQGAETIFDTDETRVVRDRAAQLACVEYGADHQSDVAIRVITDH
                     VRAAAFMVADGIFPTNEGRGYVLRRLLRRAARYGRLVGIEGSFLPQVADTVIDVMAAP
                     YPELARDRDRILKAIALEEDRFAAALEQGSVVLESIIAEVERDGGRMLPGEDAFKLYD
                     TYGFPFELTEEIASERGLQVDKAHFDEAMEAQRERARAARGENAYLDEKSAKYHDAIG
                     DLTTEFVGYHEFADTANIIAIIGKDGNSLAELSEDQDGETVMNKTPFYPTGGGQQADT
                     GLIVSSRGAVAEVCSVSRPFESVITHQVHMIRGTLKVGDRVIAYVDEDRRAATMRNHT
                     ATHLIQAALRQVLGEHVQQAGSYVDGERLRFDFSHFEALTVDELAQVELLANRWVMAD
                     IPVDVRMTTIDEAHRLGAIALFDEKYGDEVRMVGIGDISLELCGGTHVSRTGEIGLVK
                     IVSESGIAAGVRRIEAITGYAFLQRFNDMDREFQSLAEILKVPEDMMVERVEKLLDEL
                     KEKEREIARLRERLAVGGVDSLVAGRIMVDDIPVVAAPVELADPEALRNLADAVRDKL
                     GSGIVLLGSKAEEKVLFVATVSRDLVKQGFNAGSIVREAAKVAGGGGGGRPDMAQAGG
                     RDVSKLEAAIEAGVDVVRAHSAS"
     gene            complement(40498..40905)
                     /locus_tag="PLU15_01470"
     CDS             complement(40498..40905)
                     /locus_tag="PLU15_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOO29624.1"
                     /translation="MKAEIINVADLTMLAKSDTNHWVVMDSNKEGKSAAAATPMEMVL
                     FSLGGCLSMTVKSLLEKKRLDVGKMSMTIEAEREPQPPTVFTKIHASLRFEESGVTDG
                     DIEWAVNLARLKYCQVDVMLEKAVDIEIDWEIL"
     gene            complement(41050..42246)
                     /locus_tag="PLU15_01475"
     CDS             complement(41050..42246)
                     /locus_tag="PLU15_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO29625.1"
                     /translation="MRRRVGAHLHIISFSSWTVRDVDVDVASRWGAAIVRAIAIMTAV
                     VATLFVAYKARRAFVPFLIAIAISYLIRPLVMIIEARGAQRSAAIFTAYLVVTAVIGY
                     SLIFVIPPLAAQLDEIVRVFSDASGFGMDSGGSDTSLQEPTPDYKEGSKAEWRTGKGL
                     ETLVHEWGQKVTFLPERTTSRIAEAAVAGVESYIREFALAARRSVSILISQLGSIIVA
                     PVIAFYLTRDVDDIKERFASWLPQDSRDRIMGAISGIDEALAGWVRGQVIVCTFVGVA
                     TGGSLALLGVEYALPIGVLVGILEIIPYFGPLLGMVPAVLMAVQKSTLTAIIVTVAFT
                     LIQQVESAIVSPRIVGDCVGLHPLAVIGALLVGGALFGVMGILFAVPVAAVIFILGER
                     LLLTTD"
     gene            complement(42442..42588)
                     /locus_tag="PLU15_01480"
     CDS             complement(42442..42588)
                     /locus_tag="PLU15_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29626.1"
                     /translation="MVQKGGTEDSKVLEDLPHRQPYWGGCWDFDGAELISVWQGDIAR
                     GSCE"
     gene            complement(42701..43144)
                     /gene="nifU"
                     /locus_tag="PLU15_01485"
     CDS             complement(42701..43144)
                     /gene="nifU"
                     /locus_tag="PLU15_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOO29627.1"
                     /translation="MYSDKVMDHFSNPRNVGEMPNPDGVGQVGNPVCGDIMRIEIKVD
                     DGIITDIKFKTFGCGAAIATSSMATEMVKGKTLAEALEVSNRAVAEALDGLPPQKMHC
                     SNLAASAVHAAIKDYLERHKDLDLGIDPASIRIDEHDHDEVEGQE"
     gene            complement(43198..44421)
                     /gene="nifS"
                     /locus_tag="PLU15_01490"
     CDS             complement(43198..44421)
                     /gene="nifS"
                     /locus_tag="PLU15_01490"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOO29628.1"
                     /translation="MNRIYLDNAATTPVRREVFEAMVPYLTDKYGNPSSIHSYGREAR
                     ADLNRAREQVAAAIGAAPEEIIFTAGGSEADNLAIKGTALAMYGEKSHIITSAVEHHA
                     VLHSVESLKRLGFSVTILPVDSEGMVDPEDVKRAITPETCLISIMYANNEVGTIQPIE
                     EISRIAREAGVLFHTDAVQAVGHMPVDVKSLGVDMLSLSGHKFYGPKGVGALYARRGV
                     RLAPLIDGGAQERKRRAGTENMAGIVGIGMAIELAVSEMDEVSAKEERLRDKLIEGLT
                     QRIPDVRLNGHRRLRLPNNVNMSFLYVEGESLLLNLDMEGIAASSGSACTSGSLAPSH
                     VLMAMGIPHEVAHGSVRMTLGRYTEDADVERVLDVMPRIVEKLRAMSPIYVKTECGIK
                     EFCANAHGCDVAEKR"
     gene            complement(44414..44884)
                     /locus_tag="PLU15_01495"
     CDS             complement(44414..44884)
                     /locus_tag="PLU15_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO29629.1"
                     /translation="MKLSSRTEYGVRAMVELAEVHGSGPVALREVAEKQGISEKYLEQ
                     LFAALRKEGLIYSQRGAQGGYTLAKAPKHITVGDIVRALEGPIALCDCLADEEGEAIC
                     DRMGECAAHPLWVRLQDGITSILDSTTLQDMIAGDLRDSAKDIGGNKEEERPDE"
     gene            45703..48789
                     /locus_tag="PLU15_01500"
     CDS             45703..48789
                     /locus_tag="PLU15_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO29630.1"
                     /translation="MKRSKRLVIQRLALSAILLIFAATGPAHWGCPSFCLAEASSATA
                     NDDATIVGLADLRKCDEQGVPLLIDQVVTVEGAALIDTGKWHNAANYFAIVGPAQPVS
                     LSDWAKAVTPSDRVFGTLVYLPGTTVPQVKAGDFVRVTGKVSIKGYTTDYGTTVIVPS
                     SSEQIVILEHGSDIIGNLPATRLATDPTFAEAEPLEGQLVTVEGRLFGYDDEGITQGF
                     WLDGSRDGNIEDAEGMMQVKFYNYHGLDISHLGNGSYAKVTGVLVQGQDFPPYHGGYY
                     IRPTAQEFITDDPSRKLVSAESEMRAAKIRYNVGDPVHMSKKGIFQLSTMVSSTSYPF
                     VDRVHSHWNPDGSGVVFTMGKLKSARSTLENTETNLFLIDFRGGNEHSQLTFDKSPKE
                     FPRWSPDGSRIAYSALESPDELFGKWDIWISNASDNSDPIKVTDGLCYDTYPSWSPDG
                     KSLVFQSDRSGNWDIWRIDLDGSVNDGSAIRLTRSMSDDGCPDWSPDGSRIAFQSDRN
                     GNRFDIWIMSLESPENHESPEKNAFCLTGSLSGDSVCPRWSNDGTQIAFMSNHFGTWD
                     IWMADVATGDLWRVTDLEGAEKYPSFSHDGRRIVFAADLGQGWNLYAADLKRAKPAKF
                     TGSEGQAITPPQAPPEQPKAKPRGEYGIVSPQLALPAFVKPEGTLSIEVISPVQDSPT
                     SATSVEWKIRLFPKIGSSEASAISLTPHKAIALPDGRWSMEATIPEDTPINLYDLAVS
                     ALCGNTLLMEDRQPNAVSITTVDPEDSVFAIISDIHLNNPKSSGDDPDASLNTLLLEV
                     IEELNLIQPDFVVLLGDLVESNADTYHRDFDDMWNILDGYAKFPVFAVMGNHDGQRVG
                     EVDGFEYFQRHFGPLYHSFDWGGWHFALVNTYDHPAHPSDNGVIGREQLKWLDSDLHF
                     ASSAGQKIAVMLHHNPFDDRWIFMDEGREQLVEVLERHGARYVFAGHRHSDQLEQTEL
                     TKIITTRTAQAASRDMAGYRVIRIEDGYIASVHYMMPKSSKPLGTTNMGPEAEGRD"
     gene            48988..50745
                     /gene="iorA"
                     /locus_tag="PLU15_01505"
     CDS             48988..50745
                     /gene="iorA"
                     /locus_tag="PLU15_01505"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOO29631.1"
                     /translation="MKSLMTGNEAIARGAWEAGVRFATAYPGTPSTEILENIAGYDEI
                     RSQWSVNEKTALETALGGSIAGARTICAMKHVGLNVAADPLFSSAYMGVNAGLVIVTA
                     DDPGCHSSQNEQDNRNYAKAAKLPLIEPSNSQEALDFVKVAFELSEAYSTPVLFRITT
                     RLAHGKSVVTVGRRVDVKPREYVRDTSRFVLMPAQARARHGVLEDRLIQLEDWACTTR
                     LNRVEWGSRKLGIITSGIAYHYAHEVFGEDASYLKLGMSYPFPKKLVENFAAQVDRLV
                     VIEELDPFIEDAVRAMGIEAEGKAIIPRLGELDSAIIAEAFEKADSKSPPPEDVTGRP
                     PMFCAGCPHRGVFYELGKLPVTITGDIGCYTLGSLPPFAATETCICMGASISAGLGFQ
                     KVFNMLNPADPKRTIAVIGDSTFYHSGMTGLLDAVYNDVGLITIILDNRITAMTGHQE
                     NPGSGYNLSGQSAPQARPEEIAKALGVERVTTVNPYDLTEVKKALDNALADPHPSVIV
                     SRAPCALLKREAVRRPPYFVDQDACRKCKACLKVACPALYVEDGEVHIDNASCVGCGV
                     CAQVCRFGAINQTEKTGEN"
     gene            50745..51323
                     /locus_tag="PLU15_01510"
     CDS             50745..51323
                     /locus_tag="PLU15_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOO29632.1"
                     /translation="MANTTSIMLAGVGGQGTILASRILAQLLLDKGYDVKMSEVHGMA
                     QRGGSVTTQVRYGEQVDAPTIAPGQADFILSFEAMEAARTAHFLKPGGTIVANTRRIP
                     SLPILIGKEEYPDDIDERLAERGIDVRWVDAYSIAEELGNTRVENVVLLGAFSTVLPF
                     TEGAWKKAIAQVVPPRHVELNVVAFQRGSRLT"
     gene            51326..51643
                     /locus_tag="PLU15_01515"
     CDS             51326..51643
                     /locus_tag="PLU15_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOO29633.1"
                     /translation="METPIALTVAILGITGAASLIFGYLIGRKNRFDLINGYTDEKML
                     DAAKFARWMRLCYNIMGGVLIGASALLVATKAVLAIVIACTVIVTVVLAVMTLGARRY
                     YRI"
     gene            complement(51784..53145)
                     /locus_tag="PLU15_01520"
     CDS             complement(51784..53145)
                     /locus_tag="PLU15_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOO29634.1"
                     /translation="MISGPDLFDYAAEKRGGGDRVPLAARMRPRTIDEILGQDHILGP
                     GKLLRRAIEADKLGSIILYGPPGSGKTTIAEVIALTTKADFVRLNAVTAGVADIRQVV
                     ATARDRWKLQGVRTILFIDEIHRFNKAQQDALLPSVEDGTVTLIGATTENPFFDVNPP
                     LVSRSRIFKLEPLGVDDVRCLIARALDDSERGLGYLNISIDDDALDHIASFANGDART
                     ALNAIEVAALSTPAGEDGRIHITLEVAEDSIQHRALRYDGGGDMHYDVVSAFIKSMRG
                     SDPDAALHYLARMIESGEDPRFIARRLMIHASEDVGMADSQALLVASAALAAVEKVGL
                     PEARIILAHATIYIATAPKSNSVIRAIDAATDDVRTRSISDVPLHLRDASYRGASTLG
                     HGVGYKYPHDYPGAWVSQQYMPEGLEGRRYYEPGENPNEARIRQRMAEMKKGCDGSDD
                     RLR"
     assembly_gap    53297..53396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(53575..53756)
                     /gene="ssrS"
                     /locus_tag="PLU15_01525"
     ncRNA           complement(53575..53756)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLU15_01525"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(53876..55150)
                     /gene="hisS"
                     /locus_tag="PLU15_01530"
     CDS             complement(53876..55150)
                     /gene="hisS"
                     /locus_tag="PLU15_01530"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOO29635.1"
                     /translation="MDITAPRGTTDILPDEVGLWQHVEGLVRGAAERAGYREIRTPIF
                     EHTELFERGVGEATDIVEKEMYTFTDRGDRSITLRPEGTAPVVRAYVERGFASKPQPA
                     KLYYLGPMFRYERPQAGRYRQFHQFGLEIIGAPSPAADAEVISVPLEVFDSLGIRGVI
                     VNINSIGCPSCRPAYRELLRETYRPVLSKLCPSCVSRYDRNPLRLLDCKSEECRRALP
                     EPPMVLDILCPECRSHFDEVSRLLDKLNVEYVINPRLVRGLDYYTRTTFEYNVGGIGS
                     QDAIGGGGRYDGLVEEVGGPPTPAVGVACGLERCVLAMRASAGEKAYDRPIDIYFVSL
                     GEAARERAFELAFNMRSCGISADYDMLDRGLKAQMRFAAKVGARFVAIIGDEELATDM
                     VTLKAMATGDQELVLQAELASKVLEAGKAGME"
     gene            complement(55222..56421)
                     /locus_tag="PLU15_01535"
     CDS             complement(55222..56421)
                     /locus_tag="PLU15_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOO29636.1"
                     /translation="MISGMRKQAGTIVAVIAGVLALGMVIPMLFAGSNANAGSARLTV
                     AKVNSVKVSALDLALEFQQAYSRRVQYGGLKPEDIEELRSDALDALIEDALILDAAKK
                     AGYVANQVVVDSAYEADKAYFSDEREFVSALANWGFTKKSYKEYLGRQQIIGSYPSVA
                     KPVEVTEEEIKEEFDKIAVTQPNLDYEESKENIEQLIRYRKETANRQELVDELRKDAK
                     ITIYDPRVLAYRAMVEGDYDEAVKQYRKAIKQTPQDPYLQISLGKALAAAGKAKDSEK
                     AFESALKLGPDEPFVLIARADNLRESGQKDRARELYKKASDLAGEDATFSPIVHGLIR
                     DAYKEMGMDDDAAREEEMIAEIEAASRARWEAQLKEALEAAKEAKDADDAGEQVESTG
                     ESVSVEE"
     gene            complement(56485..57141)
                     /locus_tag="PLU15_01540"
     CDS             complement(56485..57141)
                     /locus_tag="PLU15_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO29637.1"
                     /translation="MADAGVLNVERLVLGPISTNCYIVWREGHEDAVVIDPGAFSPRI
                     QNILKQHGLECRAIINTHGHPDHIGGNGALRDATGAPVYVGALDADMLAEPDDVFHVL
                     ARSGGQGSSLVADHTLEGGNVLRVGEMEFEVLATPGHTPGGISLLLPDDLTLFTGDTL
                     FACGVGRTDFAGGDEEALWRSIFEVIFSLDERIRVYPGHGPDTTIAAEKAWHGRSRIR
                     "
     gene            complement(57182..57631)
                     /gene="dtd"
                     /locus_tag="PLU15_01545"
     CDS             complement(57182..57631)
                     /gene="dtd"
                     /locus_tag="PLU15_01545"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOO29638.1"
                     /translation="MRAVVQRVTEASVSVDGEIVGSTGHGLVVLLGISREDGSEDVEY
                     MVEKIPNLRIFDDDEGKLNLSLLDVGGGMLAISQFTLYGDCRRGRRPSFTDAAAPEHA
                     NVLYEEFVRGVRNMGVPVETGIFRAEMDVHLVNHGPVTILLDSGKAF"
     gene            complement(57720..59882)
                     /locus_tag="PLU15_01550"
     CDS             complement(57720..59882)
                     /locus_tag="PLU15_01550"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOO29639.1"
                     /translation="MVHGQNEPRTLEELLAKAGANRPDADLSVIERAWDYARDAHDGQ
                     VRESGEPYFDHPVAVAFILADMNLDPNTIAAGLLHDVIEDTGRSYEEISQVFGSEIAT
                     LVDGVTKLSRMDFKSREEQQAESLRKMFVAMAKDIRVILIKLADRLHNMQTLDFSPPD
                     KQRRVAGETLEIYAPLAHRLGMWRMKWELEDLSLRYLDTDAYYDLVEKVATKRQEREG
                     YIEEARETLEAALTEIGISAEIQGRPKHFYSIYRKMEKQGKDFSEIWDLMGLRVIVGT
                     LRECYAVLGAVHSIWKPIPGRFKDYVAMPKSNMYQSLHTTVIGPRGEPLEIQIRTWEM
                     HRTAEYGIAAHWRYKEGGGGGDFEDKITWLRQILEWQKETRNGTEFVETLKVDLFSDE
                     VYVFTPRGDVKTLPAGSTPIDFAYSVHTDVGHSCSGARVNGRMVPLNTKLATGDIVEI
                     ITSKSQAGPSRDWLAIAKTSKAKGKIRQWLKEEQREASVEQGKELVEKELKKLGLQVR
                     ANMKEERLLEVAQKFSYQSTDDLLASVGYGKLSAAQVVTKLVPERTADETPKPSPVKK
                     KAGSSHGVRVPGVDNLLVRFARCCNPVPGDPIVGYVTRGRGVSVHRKDCPNAMIIVNA
                     EKDRVVDVYWDTKDGESYPVEIEMEAYDAPGLLTGVINTAVEMKAHVSAVTARTLKNR
                     VAVINMTVEIHDVEHMDSVMERLRRISGVQSVRRAAIN"
     gene            complement(59967..61691)
                     /gene="recJ"
                     /locus_tag="PLU15_01555"
     CDS             complement(59967..61691)
                     /gene="recJ"
                     /locus_tag="PLU15_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOO29640.1"
                     /translation="MAPRRTIWELREGDSAAEKRLAATCGISPVLARVLANRGIVDEK
                     SVGAFLMPDPSEMHDPFAMPDMEQSAARLWAAVEIGEGIRVYGDYDVDGITSVALLVS
                     VLRSLGANVDYHIPNRLVEGYGLNREAIEQAAGDGVSLLVTVDCGVSAIEEVDYASEL
                     GIDVIITDHHEAGSILPSCVGVVDPKRHDSRYPFKELAGVGVAFKLAQAVWTLAGRPP
                     CAADPHKYLDIVALGTIADVVPLQGENRIIAKAGLELMGRSSNHGINALLDVSDLSGR
                     EIRAGHVSFTVGPRVNAAGRLGDPSLGARLLLTDSAQEAAELAAMLDEENRKRQEIEL
                     SILREAWVPVSKMNTQTTRAIVLGSDRWHSGVIGIVASRIAELTYRPTVLISFEGDVG
                     RGSARSIPAFNLYSALTECSDLLLKYGGHSQAAGLSIKAEDLDLFRERLNEIGHEWLT
                     EEDLVPRVLVDAELGEDEICIELAMELERLEPFGYGNPTPLFVTRNMHVLERRNVGPD
                     GKHLKLKLGRGGRVIDAIGFRMAGSHVDVSQGFSEVDVLYSLEVNEWNGRREPQLIVK
                     DLRKSQDA"
     gene            complement(61767..63515)
                     /locus_tag="PLU15_01560"
     CDS             complement(61767..63515)
                     /locus_tag="PLU15_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO29641.1"
                     /translation="MNIDALLSFAREINGELDLGRGVRIGETALVSRCVHVLDPHCPG
                     QAQVQCRLDAGQVFYQRSSTDEVVHIDIFHELADVDHRTAAEAIRRAVEAYAQQMSVL
                     PRPLLELGGPIWSIAGLADFIDVQTATGGGRVTGSLNFGQKESLKIAHRGHVHVAAFL
                     PPEMVGLVYYIVREVEASIVSSGVGLRRLEQIENASSSESSPVDLSPYSCSTDSYMRE
                     QIPYDGGNSLPDLMKQALEVCTDFGSVNSFKSFLECVAGPGRRENPYAMLEREFGDAE
                     GMLAKLRTLEIVEGEGAALVLTAKGRRLASFLKKFAREVENQVRRIIRRSARPRGTPL
                     ERSDFSKGMRAGNGPGIVRTVIPASEVDYRGNISVAETVRSWAVRCATEGSHSPIQRE
                     DIRIEQVRRPARVDVCLLIDASASMAGRRIQAAKHLARHLFLTCRDKVSVLTFQDRKV
                     TQHVLRARSIRALEDGLATVRPAGLTPLAAGIVEAVKLLGSRKGVPTMLVLLTDGIPT
                     MNYWTGDPARDALVAAEQIARNKLPFTCIGLAPNRGFLRKLTEIANGTLYIVDEFDRD
                     LLAKLVKDERERVQTW"
     gene            complement(63512..64342)
                     /locus_tag="PLU15_01565"
     CDS             complement(63512..64342)
                     /locus_tag="PLU15_01565"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29642.1"
                     /translation="MNAPGSWPGRLTAAALLAPQVEFPDDLVELLATLYVDFDIESIR
                     ALESAKSAARCRAALAGRSCVCLEDIRIILPLALRHRVDVSALANIMRYVDALQERAG
                     HGQGRPNATAGYGGLARSEADKSRVSKTASVADTCETLATWDTTRAPLDAANTSGRDR
                     NLGSEVATSKPNGADCGGPGLRGILHRIMERMKSEDRLVGKEGASADGGDRHCQAQGG
                     EEDADDVGVSGEGGRWGYAGGTRAKAGSASVSEVCPPNPARPITDIGPKLVFSEDDLA
                     "
     gene            complement(64535..65209)
                     /locus_tag="PLU15_01570"
                     /pseudo
     CDS             complement(64535..65209)
                     /locus_tag="PLU15_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
     gene            complement(65787..66386)
                     /locus_tag="PLU15_01575"
     CDS             complement(65787..66386)
                     /locus_tag="PLU15_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO29643.1"
                     /translation="MHNMAAIGFTEHGYRHAGLVSSIAHNVLERLVYPARTAELAGIA
                     GYLHDIGNSVSRDHHGQSSAFIAMSVLTRMGMDPDEVAIVMAAVGNHEEEYGHPVNEV
                     AAAVILADKSDVHRTRVSKRDIASFDIHDRVSYAAERSFLEVDAEQRLITLTVTIDTE
                     ICPVMDYFEIFLTRMLMCRRAAEVLDCEFKLEINGVCLL"
     gene            complement(66579..67361)
                     /locus_tag="PLU15_01580"
     CDS             complement(66579..67361)
                     /locus_tag="PLU15_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29644.1"
                     /translation="MTNKRWTALGALLILLVAIPFSAGASSTATAEEVAREYLMAFQE
                     GDWRRAEELADDAALSWVEYVGSREGKSDGIVSIAAVETANLGDSVRAKVYYTNSDGQ
                     LRVRYLKLRDIAGRFRVVDDKMLGGEWISLSYRKGLFPTAKEIGGVRVRVVGMLEVPP
                     EIKFDLIVENVSGPDNCLIYPSLEAFYVVEADGAIRGKYFAQPPAVVPEKPLPKGVAM
                     RTYAIMPFWSVDPKFSGRTIKSLSWTLFIPFGPIDQYAFDYI"
     gene            complement(67395..67730)
                     /gene="yajC"
                     /locus_tag="PLU15_01585"
     CDS             complement(67395..67730)
                     /gene="yajC"
                     /locus_tag="PLU15_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOO29645.1"
                     /translation="MEAQAGQAPSLLGALLPFVVLMLLFYFMIIRPQKKQQQQRDAMI
                     AGLRKGNRITTIGGIYGEIVDIKDDAVLLNVASQGERLIVRVAKWGVQDVLGKDNVAK
                     DKETEEEIS"
     gene            complement(67768..68874)
                     /gene="tgt"
                     /locus_tag="PLU15_01590"
     CDS             complement(67768..68874)
                     /gene="tgt"
                     /locus_tag="PLU15_01590"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOO29646.1"
                     /translation="MEFTIEKRSEETMARAGVLSTGHGDILTPVFMPVGTQASVKTVS
                     PDELSQIGAGIILSNTYHLYLRPGHELIAEAGGLHSFMSWDGAILTDSGGYQVFSLGP
                     LRTITERGVEFRSHIDGSPHMFTPERVMEIEMALGADIAMCFDECTPYPCDYEYARDS
                     AAMTARWAERCLEAHSREDQALFGIVQGSVYPDLRKRSAADISSLGFPGYGIGGLSVG
                     EPKDVMYAILDETVPCLPEDKPRYLMGVGSPDALLEGVARGVDMFDCVLPTRVARNGT
                     VFTREGRIIVRDAAYAEDFRPLDPGCDCYACRNFSRAYIRHLLKAGEVLGIRLTSIHN
                     LRHLVRMMEEIRSAIIKGTFSEYRKEQWRIWYNN"
     gene            complement(68944..69966)
                     /gene="queA"
                     /locus_tag="PLU15_01595"
     CDS             complement(68944..69966)
                     /gene="queA"
                     /locus_tag="PLU15_01595"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOO29647.1"
                     /translation="MRVEDFDYELPEELIAQTPIERRDASRLMVLRREDNSIEHRSFC
                     DLPEFLHSGDVLVLNDTRVIPARLWGTRSTGGKVELLLLQEIEADVWECLARPGKRAH
                     VGEKLSFGKGRMIGTVLDKTEYGGRVVSFEAIEGVDKVVDEIGEMPVPHYIKTDLKDA
                     ERYQTVYARERGSAAAPTAGLHFTPELLDEIASCGVVIVRVTLHVGLGTFRPVKTEVV
                     EEHVMHSEHYHVSKEAADAINEARASGGRVVAVGTTSVRTLETVARPDGTIAPGEGWT
                     SIFIYPGYTFRAVDAMVTNFHLPKSTLVMMVSAFADREFILSAYREAVAERYRFFSFG
                     DAMLIL"
     gene            complement(70102..70986)
                     /gene="era"
                     /locus_tag="PLU15_01600"
     CDS             complement(70102..70986)
                     /gene="era"
                     /locus_tag="PLU15_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOO29648.1"
                     /translation="MKSGFTAIVGKPNVGKSTLLNRLIGTKVSIVSDKRQTTRNRINA
                     IANLPDAQVIFIDTPGIHKPHHGLGKRMVQAARTAMEEVDLILFVTDATSPARDDDRT
                     VASYIRDLGTPAWLVINKKDLVDEETLAVSERACRALGEFDRVKILSAETGENLEGFI
                     GEIADAMPEGPMYYPPDMVVDRPEEFFIAELIREKLLELTREEVPHSIAVVVEEMIPR
                     EDRDIVDVSATVYVDRESQKGIVIGAGGSVLKEAGARARLEAERLLGSQINLKTWVKV
                     RRGWRDDEAILDRLGYSE"
     gene            complement(70987..71427)
                     /locus_tag="PLU15_01605"
     CDS             complement(70987..71427)
                     /locus_tag="PLU15_01605"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOO29649.1"
                     /translation="MSGSATGTDEARVLSADTEEMLVRAAREAREAAYVPYSGFRVGA
                     AVLAESGRIYAGCNVENASYGGTICAERTAFVKAISEGERGFLGVAVCGDTPTPISPC
                     GICRQFMAEFGLDIPIIMAGEGEARSTMTVRELLPAAFTSEDME"
     gene            complement(71430..72485)
                     /locus_tag="PLU15_01610"
     CDS             complement(71430..72485)
                     /locus_tag="PLU15_01610"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOO29650.1"
                     /translation="MRPRRWQILLIVFLVITAGVAFIRFWHEAKHIGRGASPGSFFRQ
                     DEADQPIRVDPLTGLPPLQDAEYFAAVIENAPAARPQRGLGAASIVYEMLAEGGITRF
                     LAFYSGRSSVEIGPIRSVRPYIVDIASGHGTVLAHCGGSQEALAIIAESNYPSVNELV
                     QGSAFYRDNTRRVPHNLYAKAESILDCARRMGYRTDFAESGYVFRKREARGNSNPIEV
                     TIRYSPGYSVQWEYDAKTSLWLRSINKKPHVDANTGQQIAAANVIIEVVPTRVLDDEG
                     RLSMELVGSGEVVALSAGEVMCGRWVRLSGTTDPAVYVAETGTPIELCPGNTWVQIVS
                     EANMVEINSGQGELGGG"
     gene            complement(72482..73600)
                     /locus_tag="PLU15_01615"
     CDS             complement(72482..73600)
                     /locus_tag="PLU15_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOO29651.1"
                     /translation="MTTLAYRRTAPNDQPVKWRKVRRPRVVRMGELDWLALLLLASIV
                     LLFDALVIGRYVGLIRLPGELTALDMARLGAMGYVDMQRTMLERDEFAGIGSAEPVLT
                     AAAQNLIAARNVEDIGRELAQAAADLHEAVQSDRLSSARGRVLELLSSDSGVQGYTGK
                     GAYILITPGASESQVQDRQNVLGAATREALSADPLIARSPTAIEVVIDQSGISFDTGD
                     LATQADLLAREIDSIHEEMDALREAAGLSELEGEGVIVLAYDAPSGYGFDEIVHQRDV
                     SEILDRLFAAGARGAQVGGERIISTSSIRCIGPIVLVNQRPVAVNPIRIYAVGNPYAL
                     ERALRPMASEFAKTGKRLEIKRESSLVLSAYHKGGLQW"
     gene            complement(73563..74621)
                     /locus_tag="PLU15_01620"
     CDS             complement(73563..74621)
                     /locus_tag="PLU15_01620"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOO29652.1"
                     /translation="MASKSKTETTKLVRRKRQWLTGIIIILLFVNILVLARAAELISF
                     PGEVSNTELLRRGAMSLADYYEAQAVKLGLDRNTAVRDALAKFKFEVSKANDAESIAY
                     IIGYYGRATGDVIEREQENRRREAVLGIVNSDPQVIAVTGQAMITVSAGQDGRIVVED
                     TAHVLSLSAIDAIQKSPVLTDLAAPIDIEIAGGKANVITPRTMQDRVRLLRDETDSLR
                     LSLDELRRAAGYSALSGSGILVSMYDAEGGFTSDEVVQDKDVRDAVNELYAAGAQGIE
                     VGGQRLVATSSIRSAGPQILVNQKPIPVNPVVIKAVGDPQVLESSLDLIKNSLKRWGI
                     RVEVERYDNISLSPYRAQ"
     gene            complement(74635..75012)
                     /locus_tag="PLU15_01625"
     CDS             complement(74635..75012)
                     /locus_tag="PLU15_01625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOO29653.1"
                     /translation="MKARNLRESFRYAGIGISQAFRSERNIRVHWIAAAAVGIVSILL
                     KLSVQEAGIIALAVAMVITAEMVNTAIEHCVDLVTSEYDPLAAEAKNVAAGAVLVSAV
                     GAAIVGAIILGPKLFSAILSRLR"
     gene            complement(75028..75576)
                     /gene="ybeY"
                     /locus_tag="PLU15_01630"
     CDS             complement(75028..75576)
                     /gene="ybeY"
                     /locus_tag="PLU15_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOO29654.1"
                     /translation="MPAEILNRQDKVEVTDELEAQVEEAVLIALQTVLGDDNYEVDVS
                     FVDDAEIARLNETYRGIQAPTDVLSFPLEDPDALIADDECAEITECPDWPNVIPPTEY
                     EGMDYEFAAEEDVLLGDIVISLERAESQAKDYGHSLSREVCYLTVHGVLHLSGYDHEN
                     DEDAARMRGLEESVMNRIGLPR"
     gene            complement(75610..77817)
                     /locus_tag="PLU15_01635"
     CDS             complement(75610..77817)
                     /locus_tag="PLU15_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO29655.1"
                     /translation="MAQLSRKESARRFGSSVKTFASSRIAVAVATVILGSLIVASALL
                     PQAVTLEEGQFAKRDIQAQRTIVNRVATEKLREEARKTYLKNAPSFPDNYEINQSCSY
                     MSEEAVESVFSAVDSARSEMPEEEDLAAVGRRAAAIIDDGEGISVSAEDLALLASMDE
                     AEREDARDIAVALVGSVMRENRITDSNLQEIAAGIPEALLEKGVAPEYVAPISRIVVS
                     AIRPNLSLSLAKVEKAAEEEADLVQPVYVQKGQNIIRRGEPATQETIEILRDLGLLGA
                     RGSILGWVGVISTTALAVGYVAIYLQNFPAHAPNEQGMLSLVAIAGLLVLLLASILAN
                     LTPDYAAYLVPAAFATMIISFLASLEMALVVNLSVAALVGVLLRGNMFAMLASLAAGA
                     AGAYSAHDLHERANMTRAVVAVGTAVAAVSVCYGLAFGEPEIVSRWYLGFVNGIISTV
                     MTLGSLPFFETLFGVSSSLRLLELSNPGHPLLRRLLLEAPGTYHHSILVGNLAETAAQ
                     AIEADALLVRVGALYHDCGKLKRPYFFVENQFAQANPHDKISPALSTLVITSHVKDGV
                     ELARQYKLPAPVISIIQQHHGTGLVLFFYARAAESERDLCIDEKDFRYPGPKPQTREA
                     AVVMLADSVEAAVRSMSNPSSWQVQNTVRKIIKDKLSDGQLDECDLTLRELSEIEQAF
                     ISVLSGVYHERVEYPELPPHEPGTDRELEEVKVAAQDGAGESSPEQGDNRVER"
     gene            complement(77853..78863)
                     /locus_tag="PLU15_01640"
     CDS             complement(77853..78863)
                     /locus_tag="PLU15_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO29656.1"
                     /translation="MSNPVVEARFCTNDNSEAAALFGNRDENLRLVEELLECKIVARA
                     NDVTVIGPEDSVSKVMRVLSDLQSAVRAGNRISGRETRYSVMLAQNERPETLCSVLRD
                     VVQVTARGKQVRPRTVGQKHYVDAMRRNDIVFCIGPAGTGKTYLAMAMAVSVLKAKNV
                     SRIILTRPAVEAGEKLGFLPGDLQEKVDPYLRPLYDALFDILGVEMFERYKEKGIIEI
                     APLAYMRGRTLDDAFIILDEAQNTTPEQMKMFLTRLGFSSKAVVTGDITQIDLPNRKF
                     SGLEDVQTVLAGVDGIEFVHLNDQDVVRHELVQKIVQAYERYDAERAKKEQLGVDLSR
                     GE"
     assembly_gap    78930..79029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(79075..80232)
                     /gene="yqfD"
                     /locus_tag="PLU15_01645"
     CDS             complement(79075..80232)
                     /gene="yqfD"
                     /locus_tag="PLU15_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOO29657.1"
                     /translation="MATSGLWDWVRGYVIIRVTGRMIEKFVNMAAARHIGLYDVTTLG
                     PGMITARVSVDDYRRLHPILRDCQCRATVDARVGAPFLIAALGRRKFLFIGLMLFVAI
                     LYVMSSILWTIEVVGANPHQEQQIRRILDDMGVNRWTLLSSIDCDTIRSEITKSVEGL
                     AWAGVETQGTALIVRVAEKILPDAPVFPIDLIASEDAIVVKLIVLAGDACVAEGQTVV
                     AGQVLVSGKSASGAGGDAAHARAIVEGRVWREGRAVVPLNVEERVRTGRKSIHYRVLW
                     GNLDFRLGRVGSFEAFDTKEYRRDYRPGVEFIRIERYELALTSTRISRSEAYALARAA
                     AEDSIAAKLNKDARIVSVSERVSDENVMPEAVEVILTVETVEDIARPRERQ"
     gene            complement(80234..80527)
                     /gene="yqfC"
                     /locus_tag="PLU15_01650"
     CDS             complement(80234..80527)
                     /gene="yqfC"
                     /locus_tag="PLU15_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HOO29658.1"
                     /translation="MREARSARIKERMSDALGVPKDVALDLPRLSLEGNARLVMENHK
                     GLIEYSADRLCIQTTVGQVSIVGRKLVLRGINRERIVVEGRFDSLEFLGWEVS"
     gene            complement(80643..81944)
                     /gene="purD"
                     /locus_tag="PLU15_01655"
     CDS             complement(80643..81944)
                     /gene="purD"
                     /locus_tag="PLU15_01655"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOO29659.1"
                     /translation="MSSLSTRPNILVVGSGGREHAIVWKLAASDRVGRVYSTPGNAGI
                     ERLGQCVPIDVADVEGLARFAQEADCHLVVVGPELPLSLGLADRLAAQGIAVFGPTAS
                     AARIESSKAYAKGLMERAGIPTARYGVFDDISKARQAAVAIASDAGRVVVKLDALAAG
                     KGVIVASGPEEAGAAVDSLAESAKASNCRLLVEECLEGYEASVIGITDGKDVVTLVPA
                     RDHKRAFDGDEGPNTGGMGAVAPAPGIDRAMLDWISNKVLLPAVRQMADEGDPFVGAL
                     FAGLMITDEGPKVLEFNCRFGDPETQAIMPLLESDLLDLVEAACHGRIGEMKADFVPG
                     YSTCVVLASNGYPGPYSTGVPVRVPSELPEGTVIFHAGTRISEGQLVSDGGRVLGVTA
                     VGSTQTESRRRAYDAVERIGFEGGYCRSDIGLDVRFTGGGL"
     gene            complement(82069..83622)
                     /gene="purH"
                     /locus_tag="PLU15_01660"
     CDS             complement(82069..83622)
                     /gene="purH"
                     /locus_tag="PLU15_01660"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOO29660.1"
                     /translation="MARGRALISVYDKEGIEEFARSLAELGFEIISTGGTAALLRKAG
                     VDVRDVSEITGFPEVLDGRVKTLHPRIHAGILAKPTREHLDALNRLDVTPIDIVVVNL
                     YPFERTVEGGAEMEEACENIDIGGPSMARAAAKNFERVTVVVNPRRYPEIISELREHG
                     EVSRATRAALAAEAFLHTAAYDAAIANYFAEQTEKAEDEGKSLGDHWVIGGTKAQALR
                     YGENPHQAAAFYKFSGGAPWGLAAAKQLGGKELSFNNLADATGAIGVVSEYDETAVVI
                     VKHANPCGVGVGKTPLEAYKAALDCDPVSAFGGIVAFNRSVGIEEAKLMSEIFLEVII
                     APEFSPEAVELLHGKKNLRLLEIAEEDLKPRAGWDIRPVLGGFLVQNSDSAKAVPLDL
                     SVVTKRAPNEEELAQLVNAWKTVAHVRSNAIVLWRGNGTVGVGAGQMNRVGAADIAIR
                     QAGEKARGSVMASDGFFPFGDTVRLAAEAGVRAVIQPGGSLRDSESIEAADANDIAMV
                     FTGVRHFKH"
     gene            complement(83653..84216)
                     /gene="purN"
                     /locus_tag="PLU15_01665"
     CDS             complement(83653..84216)
                     /gene="purN"
                     /locus_tag="PLU15_01665"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOO29661.1"
                     /translation="MRLVVLVSGRGSNLQAIFDAIEGGRLDAEVAAVISDSPDAYALK
                     RAEKAHVPGFCIERRDHSSRADFEAALAACAEAARPDLVVLAGFMRVLGPVFLDRFLG
                     RVINIHPSLLPSFPGLEAQRQALEAGVRYTGCTVHFVDYGVDSGPIIDQRVVPVLAGD
                     DVERLSARILEQEHDLLVDVIRRLGCK"
     gene            complement(84220..86697)
                     /gene="purF"
                     /locus_tag="PLU15_01670"
     CDS             complement(84220..86697)
                     /gene="purF"
                     /locus_tag="PLU15_01670"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOO29662.1"
                     /translation="MRHGALRAQEADKLKEECGVFGACFREDAAVAVYYGLVSLQHRG
                     QESAGIAVYDGSRIRVVKGAGLATEVFEGDAFKRLPGKTGIGHVRHGVCQDNDVSGAQ
                     PFVFRHGGGMMAVAHNGSLTNGRVLRERLGRLGVVFQSESDSEVIGSLIARYFSLGMV
                     GAIERAMEELEGAYSFVVMVDDTIYAVRDPYGFRPLIAGSGPAGWAVSSESCALDAVG
                     ITEKRDVGRGEIVVISHDGMKRIATRASRRSCPSATCIFEYIYFARPDSIIEGIGVAA
                     AREAFGAMLAREARFDADVVVAVPESGVTAALGYSDESGIPYRQGLTKNRYMGRTFIR
                     NSQMERELGVRLKLNAVERSCAGKRVVLVDDSIVRGTTGARLVDLMRKAGATRVDMAV
                     SSPPVKFPCTYGIDTASRRDLVAAVKSEDEIREMIGADALHYLSIDGMLEAIGGLASA
                     RDTGEPLEFCTACLDGRYPDAISCHGKCRPPKSLSYADSGVDIDEGERAVQLMKPAVK
                     STFTPGVIGDIGGFGGLFKPDFAGMKEPVLVSGTDGVGTKLKIAFAADRHDTIGIDAV
                     AMCVNDVLVSGASPLFFLDYIGCGRLDAGQIAEIVGGVAEGCRQAGCALIGGETAEMP
                     GFYPDGEYDIAGFAVGAVDRGRIIDGSKIRPGDRVIGLASSGLHSNGYSLARKALCEV
                     GGFLLSDIPEGMDRPLGDEMLEPTRIYVRPVLALIERVEVLGMSHITGGGLVQNPPRI
                     LPHGASMALSVDTWRRPPIFDVISRTAGVAADEMFRVFNMGIGFLVVVRPEKAREAVN
                     ILVDQGEDARIIGEIIPGDGRVEFQGV"
     gene            complement(86679..88946)
                     /gene="purL"
                     /locus_tag="PLU15_01675"
     CDS             complement(86679..88946)
                     /gene="purL"
                     /locus_tag="PLU15_01675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOO29663.1"
                     /translation="MAKTDSLKESIWRSMGLSDEEYQMIQDLLGREPNYVELGMFSVM
                     WSEHCSYKNSRAVLKGLPTSGPQVLQGPGENAGIVDVGDGLAVAFKIESHNHPSAVEP
                     YEGAATGVGGIIRDIFTMGARPIASLNSLWLGDLQSNKSRMLFDGIVSGISGYGNAVG
                     VPTVGGQTVFHQSYENNPLVNAMCVGVMRQDEIKRSAASGVGNSVMLIGSTTGRDGIH
                     GATFASADLTEEAVEKKSAVQVGDPFREKLLLEACLELVARGCVLGIQDMGAAGLTCS
                     SCEMAARGNVGMVIDIDKVPRREDGMTPYEVMLSESQERMLLVPVPGKEDEVKNVLEK
                     WDLLAEEIGKVTDDGFITLLEGGEIVAQVPAKALAENAPLYHRESVRPKYLDEVALYD
                     VGALEAPKDLGGALLQLLGSPNLASKKWVYRQYDQQVGAGTVVGPGSDAAVVRLPGTS
                     RGIALTCDCNSRYCYVNPRLGAAHAVAEAARNLACTGARPLAATNCQNYGNPYKPEVF
                     WQFKEGIEGMAEACIALNTPITGGNVSFYNEDGEMAIRPTPVVGMVGVIDDVEDCVAM
                     GFSQEGDVIVLLGTTRNEIGASEYLATVHGVEAGQVPELDLDVERRLCELLLAAADRG
                     ITRSAHDLSDGGLGVALAECCIAGSCGARVRLEANIAPDRFLFSESAGRAIISVDESD
                     LGALLDLADEMGVAAKAIGEVGGESLDITCVESACVPVQGDDRRAQEAEVVIALPVAK
                     LAQVYEEAIPCAMER"
     gene            complement(88930..89643)
                     /gene="purQ"
                     /locus_tag="PLU15_01680"
     CDS             complement(88930..89643)
                     /gene="purQ"
                     /locus_tag="PLU15_01680"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOO29664.1"
                     /translation="MKFGVIVFPGSNCDRDCRNAIEAAGFDAEFIWHRDTSVAGYDCL
                     VLPGGYSYGDYLRPGAIARFSPVMDSVVEYARDGRPVIGICNGFQILLEAGLLPGAMQ
                     RNVGTRFICDRVRVRIESTRTPFTSGCREGDVLTLPIAHADGNYYADPQTLAELEERG
                     QVVFRYCNELGDATPEANPNGSLNNIAGVSNEAGNVVGMMPHPERACEAILGGTDGRR
                     IFESVAEWVRGGVRVGQDG"
     gene            complement(89640..89969)
                     /gene="purS"
                     /locus_tag="PLU15_01685"
     CDS             complement(89640..89969)
                     /gene="purS"
                     /locus_tag="PLU15_01685"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOO29665.1"
                     /translation="MAKSLRPKHEDDAQTKVVLEVHGVKYVAYVDVQLKQSILDPQGE
                     AVRSALVQMGYGGVQKVRIGKRIRVDLEAANEAECRRIMDEICSQILANPTMETYEYE
                     IKEESGK"
     gene            complement(90006..90545)
                     /gene="purE"
                     /locus_tag="PLU15_01690"
     CDS             complement(90006..90545)
                     /gene="purE"
                     /locus_tag="PLU15_01690"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOO29666.1"
                     /translation="MSRSEKEPGATCARPRVGVIMGSDSDMGRMLKATEVLSALDIEW
                     EMRVISAHRTPDDAAEYAKSAEERGLSVLIAGAGMAAHLAGVIASHTVLPVIGVPLSG
                     GALGGFDALCSTVMMPSGVPVATVAIDGAANAALLAAEILAVSDGELRKRLLEHREAL
                     AHKVRLADAKAQAPQDGAE"
     gene            complement(90921..91577)
                     /locus_tag="PLU15_01695"
     CDS             complement(90921..91577)
                     /locus_tag="PLU15_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29667.1"
                     /translation="MDMSDLAALVVWIVVIFSYVLRIVARGFKQAEEEALKKARHLEA
                     SRDAQAEGRMEPSAPADGFAGSPEVPSTLQSYELESVEAEEDYDAWPLPKTRRSLAQE
                     AAEERAYGREVEMAEPIVTGSLGEAGDDASEWSERTIEPRATDVDDVILDLEIETSLE
                     ASRVFERELVQEWEADVVSPQLTSDGEAFGKQVVSGIIWATVLGPPRCRKRAGTTRIP
                     "
     gene            complement(91678..92679)
                     /gene="floA"
                     /locus_tag="PLU15_01700"
     CDS             complement(91678..92679)
                     /gene="floA"
                     /locus_tag="PLU15_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496556.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOO29668.1"
                     /translation="MAIGWIILIVVLVLLLVLLNFIPVGLWISAWAAGVNVGLLTLVG
                     MRLRRVPPVRIVLPMVKAAKGGIPVTVDKLEAHYLAGGNVDRVVDALIAAQRADINLT
                     FERAAAIDLAGRNVLEAVQMSVNPKVIETPVIAAVAKDGIELKAKARVTVRANIDRLV
                     GGAGEATIIARVGEGIVTTVGSAESHKEVLENPDQISRTVLNKGLDDGTAFEILSIDI
                     ADVDVGRNIGARLQMDQAEADKNIAQAKAAERRFAALALEQENKAKVQEMRAMVVEAE
                     AEVPRALADALRSGNLGVMDYYNLLNLKADTQMRDSISKATGAPGSSEGSGSDAGLK"
     gene            complement(92684..94015)
                     /locus_tag="PLU15_01705"
     CDS             complement(92684..94015)
                     /locus_tag="PLU15_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOO29669.1"
                     /translation="MRAKRGVIGVFVLVLIVVGLLVPAGKCAAAPKVYIVPLQGQIEH
                     GLASFIARGLREASDAGADVVIIDIDTPGGYVDSALKMRDAIISSEVRTIAYVDSRAW
                     SAGALIAIACDDIAMKMGSSMGAAETRPKEEKYISAVRAEFEATAELRGRDPAVAGAM
                     VDADVEIPGVIERGKILTLTATAAENLGFIEYKVKDLDDLLGLLGLSGAEIVDVKQTS
                     AEWFARLLTNQVAAEVLLTLGLVGLAVELVTPGFGIPGTIGIVSIGLFFGGRIMAGLA
                     GWEVIVLFLVGLTLLLLEVFVIPGFGVAGILGIVSVIASFVLSYPSPGEGAAALGIAI
                     GIAIAVVALVIFLLSRGGRKGRAPAKLVLDHTETPEEGYLAVETYESLYGAEGIVVTP
                     LKPVGIVDFDGRRVEAISEGPFVMGGARVRVIRIEGNKVFVRPITQMDQED"
     gene            complement(94130..94591)
                     /locus_tag="PLU15_01710"
     CDS             complement(94130..94591)
                     /locus_tag="PLU15_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOO29670.1"
                     /translation="MSIAVRLRDDLKIAMKSKDELKKSVIRLALAGLKNAELEKGAPL
                     SESEEVDVMARQAKMRRESIEEYEKAGRFDAADVERKEMEIIMSYLPRQLSEDELRQI
                     ISETIAQTGAEEPRDIGKVMGAVMPKLRGRADGKLVNSMVREMLDAQGGEE"
     gene            complement(94687..96027)
                     /locus_tag="PLU15_01715"
     CDS             complement(94687..96027)
                     /locus_tag="PLU15_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOO29671.1"
                     /translation="MTADNMDYVDVSSDHGSHPLGRRLTVAACVLCVVILVFALGGCW
                     DARDIDHIGFIMGMGVDESDDGKIVVWVQMALPSSGPEGAANTPAWLGSAAGNTMMDA
                     MSALRMKSAKTLFRGHVKVLVIGEKIARKGLTPILDYLARDSQFRYNSWVLVTSDPVG
                     KIFEVEADQENMASSYINLMMRHATLSSVAPQSRFRDLLSTAAEPGDQPVLARVSLVK
                     SEPEEEEGEQDEAQQGVWGQRTGNEGGKGKKGDVETKSDIEISGSAALHGDLVVGWLD
                     PVQTAMVLMARNRLKEYSFLHMVPGTEGDTVGVKVLHSRATIELPPRHKMTRTGLRGA
                     EVRIRVTGCAYIEEVMSEEQFVSIEADRNLSRGVSDQIEAEMESLIEIAQNRLGTDIF
                     GIGEAVRRRISSDAWEEEIAPIWRDIFKEIKIIPEVSLKVERQGTTMTSPRPQA"
     gene            complement(96014..97102)
                     /locus_tag="PLU15_01720"
     CDS             complement(96014..97102)
                     /locus_tag="PLU15_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOO29672.1"
                     /translation="MYYTQVVVLVAVIVFSTGFLAAARLLTPVAGTAAWISVLLGGVI
                     GLVIAWLVGSVSSAFPEQNLVKIAEDIFGKPFGKVIGLTFALYCTVVVVLGTREFVLA
                     ISISFLERTPFYVTTAVFFILASYAAYLGIEAIARVCFIFFGVVIISIAILGVFAIPV
                     LVPGRLLPLAGRGVIPIMRAALLTASYTGEAIVSLAFLRHLKDKRHVSRAVGTGVAIG
                     TAIMAMTVAYGIMMLGTGGISRVTYPAVETARMVGAGEFLERGEILFLALWFSVAILK
                     LASVFYAAITAIADLLNLDNYRRLILPFGIISMVLSSIPANVAQSFAAVEHFLKYTVW
                     YAIALPALLLIGARMLRHEKGGTPDDSR"
     gene            complement(97238..>98982)
                     /locus_tag="PLU15_01725"
     CDS             complement(97238..>98982)
                     /locus_tag="PLU15_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOO29673.1"
                     /translation="RGKRNLTRLRRLLKGEARRPRMVLGKTPVSLDEKKSGGGEGPDQ
                     HEANETPIPDSLDGIEKFLRERLGNESPDVILRRLVIWTSPELDALLFYIEGLTSRDV
                     INMMALRSVMLAERTTALDAGALDRENIATVISTHLVPSGQITVVRSMELAMEHVLTG
                     EGALFVEGSPECVLFEAKGWEHRGIEEPATEKVVRGPREGFAELLRANTALIRRRLRT
                     PDLRLDATIVGVRSKTDVVVAYVEGLTDPDLVDTVKERIDAICVDIMTDSGVLEELIE
                     DHTYSPFPQVQYTERPDRFVAALSEGLVGILVDGSPMALMIPANLASFFQSAEDYYER
                     FPFGGPLRTLRYIAGVIALIFPALYVAVATFHQEMIPTKLALAIAGSHVPVPFPSLVE
                     ALLMEVALELIREAAIRMPDPISQSLGFVGALLLGDAAVSAGLVSPIMVIVVAVTGLS
                     SLIIPHYPTGLAIRLLRFPLLFLSASLGLFGLIAGLIAIALHLGGLTSFGVPYLEPMM
                     KPKELVRDVIWRSPEYTFQRRPEYPSPQDQIRQKAFMRTWIPEVAEKARQDGEALADS
                     RDQSGEEENTKGRG"
BASE COUNT        22605 a        30784 c        23697 g        21596 t
ORIGIN      
        1 gacgaggtac gagcctctcc ccgggaccac ttcatcccca cgaagcatct tgatagtact
       61 ggtaataatg aacgaggccg acccccgttt tcacaggtct taggctatac taaaggaaaa
      121 gcatgtctta gtgcagagct acatgtgaaa ggggccggta ctatggagtt tttccaaatg
      181 ctcttgacac aattcacaag gtgctcaagc attttgccaa taggctgtgg ccttcgcgag
      241 gttcaacaga cttgatcagt ttcaagccct ggcccttgag gaattcgatg agttccacga
      301 atttcggatg ttcgctactg aaaaactcgc caagctaaat caacgcccgg ccgcaatcga
      361 aaagagccag caggttgatc acgatatgat cccagttaga tcagatgggc attcgcctta
      421 gagctcttga agagaacgcg gatagagcag gcgcaccatc cggcacagta taggctttcg
      481 tctatgccgc ctagctggtg cgctatccca ctccaaatca gccttgatta acagctccat
      541 cctggttcga cgcttgccgt aatcctgggg gaagccacat gagcccatat gatcaagatg
      601 tcgctcctct ccaggtcatt cgttagagtc aatgaggaac atgggcggca gccatggaca
      661 gatcaaatcc cctcactgcg aaaaaacatg catccaggag acagaagttg cacgttgcga
      721 ttacctcttc tgttgctagc gatcttccaa gaaaccgtgc aggcttatcg ttacaccgta
      781 ggatttgccg ccttcgaatt cgatggcctc tatcctgcca ctgccactga cggcaatatt
      841 cttctgcttg aacctgtcca gatcgcttcc taccttgccc gtcacggcaa atgtgtagcc
      901 tgcgccagcc tggtattgaa ttcccatgat gatgagggcg ccctcgtcta cgacttccac
      961 gcctgaggca gtgcgctcca ggctcatcct tgcgccggcc gtataacgtt ttccgccatc
     1021 ctgctccagc gtaacatcga tagttgcctc tgcaatgacg gtgccctccg gaatgtcatc
     1081 caacacgcct ttcgcttcca gctccgctat tgcctctgag cctatcggaa gcaatgtcca
     1141 cttcggctcg atcatgatcg tctccgtgcc ttcctccggc actatttcca gatcgccgat
     1201 atcagaaccg tccgccttga cctcgacagc cttagtaagc gtctgaaatc cttccttctg
     1261 gacagtaaca gtctgcctcc ccgccttaac gccagagatc tggaaacgcc catcataatc
     1321 cgtaacggct gtgctaacct ttccgatcgt cacagaagct ctgtatatag gaatttcctt
     1381 tccattggca tccgtggcaa ttacccttcc atttacgacg actttaggcc cgaaccacgt
     1441 acacccggcc cctgtaaatg ccattgcaag aattattaat gaannnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnacactca ccagtttgaa actggctttg actataagat
     1621 ttccctatgc ctgattccca tctccttgcg tctcctgttt gtcgcttccc atatcaggtc
     1681 gcggacgcgc tgcgggtcct ttacaccaac caagcgaaat tccggggttg atgggtctgt
     1741 ggatatgatc tctatctggc cgatccccat gatcctatct gaaagcccct gggtcattct
     1801 gatgtccttg acgcgggcca agtcgatctg ctcttccttc cggccgagca gacctgtggt
     1861 gatgaggatc ctctggtttg ttaccacgta attcgtgttt cgcgcgatct ccttgagata
     1921 tggcttgccc tcccatagca cctcctcttg ggcagtcatg tgcctttcag gttcagaaga
     1981 gtcaaggatc ttgaggcgtg caccgcattc cttgcaaaac ttatcatctg ggttgcagcc
     2041 tgcaccacat gcaggacaat atgccataac aaacgcctcc cttattgcaa cggcctttcc
     2101 catagccgat cagcccaatt gaaaaagtgc attaccaagc aagaactagt ctacagcaca
     2161 ggcatccact aggcaaggca atgtcctcta tcacaagcag cgtacatcag aacgtaccct
     2221 acctccacca gttcactcag gttccgcttc atgggcctct cgccaagaga cttcaccctc
     2281 aaacttcccc tataccatgc ccacgcatgg ccaactatga tgacatttac ggatgtcttg
     2341 cctcacgaca aaagcacata cagcccgata tcgtgccaca gcgtctagca gaccttctcg
     2401 aagtgggcac cgcccaagcg gagggaacgc ggaatcacat ccccgcttcc gatggaagac
     2461 ctcgatttca aacggaacct ctctttcgcg caacgctcag tccatacaat aatccaggcg
     2521 ggtcagcgtt cggcatctat aagaaagggc gcgccgtccc gccggggcat aaacccccgc
     2581 gagtcggcgc gcttcaatga gccaccaggc ccgatagttg cagaaactag tacattccca
     2641 tctttgcttc ggatatatgg atgagaatat tcttcttctg agtataatgg tcgagcatca
     2701 tccgatgcgt ctcacgcccg attccggact tcttgtaacc accgaatggg gcgtgcgccg
     2761 ggatattgtt gtagttgttt atccacatac gtcccgtctc aacacctctt gcaaccctaa
     2821 gggccctgtt gatatcgcgt gtccagaccg cgcccccaag gccgtactcg ctgtcgttgg
     2881 ccatcttgat gacctcgtcc tcatccttga atttgatgaa gcagactacc ggcccgaaga
     2941 tctcttcctg cgccactctc atgtcattgg tcacatcagc caagatcgtg ggacgcatga
     3001 acgggccccg ggcaagctct ccctcttcaa tgcgagtgcc gccacagacg accttggcgc
     3061 cctcgtcttt tccgatttca atgtacttca agatcttctg gagctgtccc tcgttgatct
     3121 gacagcccat aacggtctcc tttttccagg gcaagccgac cttgatgcta ttgaacttct
     3181 caacacactc cgccaggaac ttgtcataga tatcttcgtg cacgaatact ctcgaacctg
     3241 cgcagcacac ctgcccctga ttgaacagga ttccgatctg aacgccttcg atggccttct
     3301 cccagggggc atccgggaag aatatgttgg ccgactttcc gcccagctca agtgtggctg
     3361 gaatgatttt atccgctgcc gccctcgcga tattgtaccc aacttctgtg gaaccagtga
     3421 aagcgagttt gcggaaaccg ggatgctcca gcatgtactg gcctgtagtg gagccccgcc
     3481 ctgttactac attgacgact ccgggcggaa caacatcctg tatgatcttg gcaaattcga
     3541 gaatactgag tggcgtggct gatgacggct tgatcactat ggtgtctccg gctgccaagg
     3601 ccggcgcaat cttccacgca gccatcaaga acggaaagtt ccacggtatg atctgaccaa
     3661 caacccctat cggctcccgc agtatgatgc tgagggtctg gtcgtcaatc atgactgcgc
     3721 tatcctcttc agctcttgct acccctgcaa agtagcggaa gtgatccgcc cccaacggaa
     3781 cgtcgagcgt cgcgctctcg cgaataggct tgccgttgtc cagcgtttcg atcatggcca
     3841 gtttttcggt gtttgcatcg atgcggtctg caatctcaag aagaatctgc gaacgctgtg
     3901 cgggactggt cagcttccat gaatcgtacg ctttccaggc tgccttgaca gcaagatcca
     3961 cgtcctctgg ccctgcttcc acacaggttg aaaggagctc tccattggcc gggcaatagg
     4021 tgtcgaaggt cttcccttcc ttgccgtcaa cccacttccc gtcgataaac agcttgtaat
     4081 tcctgtccat cggcttttcc atggtctatc gtcccctcct tcttgttttg accccacggc
     4141 gccccaagaa ccatttttca gatgggcacc ggttgtcatc atgcgactaa taaacaatgc
     4201 aactttcatg ccatcaagtt ttacctgctc agaaaaggca cgttgttgag aagatgttac
     4261 atagctgtaa cttaaatggt ggtatacgtg acaaagtggt gacatatatg caacaatagg
     4321 aatgagagcc atgacatgca tcttgccgcc tcttgtgtag cgcccgcctg gcgggcgcat
     4381 gcggagacgc aaagacagaa agggggacac cctaatggcc agcgagtaca ttccccacgt
     4441 cgtggcgaaa gcgtgggagg atctggtcaa taccgggaca gtgagagttg aagcactcag
     4501 gcccgttgtg gcctcctcct ggaccaggtc ccaacaggct gggatagacc cgagggatgc
     4561 agtaggcaag attgtcctca gccaggccca gctggaaaat gtcatattgg accgctccga
     4621 tctgattgaa gttgccagac cgttcatgtc cagcctttac gagttcgttg caggttcagg
     4681 cttcgtcgtg atcatctccg atgaactcgg gcgtgtgatg gaggtcatgg gagacgaaaa
     4741 cgcccttcgc cgaggagcgc tagtcaacct tctgcccggc tcgagctggg cagaacatca
     4801 cgtgggcacc aatggaatag gcacagccct tgccatcgcc aagccagttc agttatcagg
     4861 agaagagcac tattgcgaga agttccatcc atggacatgc tctgcagcac cgatacgcaa
     4921 cagcaacgga tcgatcattg gcgcactcca gatgtccggc ccttcatcga aaacccacat
     4981 ccacactttg ggaatggtag tggcggcgat tgaagcaatt gaagcacaga tgaagatcaa
     5041 cgccaaagtc cgggagctca cagtcctaaa tgacaacctg aacaagatgt ttcagatagc
     5101 ctcagatggg atagtgcttg tcaacaatga aggcataatc cagcgaacca atccggtagc
     5161 agatcggatt ctcggcacta cccgcccaac ctcgttcatg gaggcaacag gcgcaccgag
     5221 ccatttggag aagatgatct caaacggcac cgccttcgat gatgtggaaa tgagcgtgga
     5281 gaccccagaa gggcgcgtgc aatgtttggt gagcggaaaa gccatctacg atgaagacgg
     5341 cgagatcaga gggggcgtcg tatcactcaa tccaaccaag aggattgcca agctcatggg
     5401 aaggtacagc cgttcgtacg ccaccttcac cttcgacgac attataggca gagacggcaa
     5461 tctccgccgc gctataaagt tgggacatat agcagcggaa agcgacagta atgtgctgat
     5521 atgcggcgaa agcgggacag gcaaggagat gtttgcccag gccattcaca acgagagcag
     5581 acaggcaaat ggcccatttg tgcccataaa ctgtggtgcc attccgcgcg agctggtagg
     5641 cagcgagcta ttcggttatg aggaaggcgc cttcacaggc gcagcccggg gcggacggcc
     5701 aggcaaattc gaacttgcgt caggcggcac tctcttcctg gacgaaatcg gcgatatgcc
     5761 ccttgaacag caaattgcgc ttcacagagt ccttcaggac aagatggtaa cccgcatagg
     5821 cggtgcgaac atgactgaag tagatgtaag gataatctgc gcaaccaaca aaaacctcca
     5881 acatgaagtc gccaaaggca acttcagaca agacctctac tacaggctga acgtgatata
     5941 cattcttctg ccgccgttga gagaacgccg agaagacata cccctgcttt tcgatttctt
     6001 cctttcgaaa gccatgtcac aattaggagt cgacatatca aatgtcgatc ccgatgtaac
     6061 aggctgtttg acctcatacg attggccagg caacgtaagg gagcttcaaa acgtggtgga
     6121 acgcatggtg aatatcgcca catccggata catcggtctc gaacacattc ctgtagaaat
     6181 actcaaccct agccgctccg gaaacacatc gacgcaaatg ggttcacctt ttcctgcggc
     6241 aaagcctcca actttcgagc aacagcgggc ggagattaga cgcactgccg agcagcgcca
     6301 gcgagaagaa ctgattcgag ccctcgaaaa acatgatggc aacataacac ggacagcaga
     6361 agaaatgggc gtctcgagga acacaattta tagaagaatg aaaaaactcg atctgcaaag
     6421 gtgagttgtt tcctaacccc atgtcgaccg cgtcatacca atggcattgt ccatgtttcg
     6481 cccggtcctt ggacgcaacc cgatgcaggt gccgtagagc acagctgatc aagatagcat
     6541 ctacgcaccg tcgcgaagac tagattccgc ttgtaatccc acaaacgtca tgggataata
     6601 aatattgctg ccaaagcctc taacgggccg aagtctggca gtagcccttc tcggcccaaa
     6661 gcccgtccgc tgcaaaaact gggcaggcct ttgggaaccg atttggatgg accagaaacg
     6721 aaagtccgca tattctcacc ggaccacgta tgggcaggtg aagatgtgcg gagaaagcag
     6781 gtgaacattt tgggcttcgt tctatcggag aatgctggcg ttgccgtcgt aggaatcaaa
     6841 acagggctga taatgcctac cgatgacata gtcgaagtca ccatggacgc agtcgaggat
     6901 attctggagg acggcgatat cgtatgcgtc accgaggcgg ttgtggccag atctcagaat
     6961 cgctacataa cctgcgatga gcttgcagaa gacgtgaagg ccaaacttaa cataaaagat
     7021 aacggcactg tcgccgtcat aagtcccatc gtcagccgca atcgtttcgc gttggttctt
     7081 caggccatcg cgcgggcggt aaacaaagga agagtcatag tacagctgac agtcccgtgc
     7141 gacgaagtgg ggaaccaagt aatagacgag gaattcgcca ataaccgtct tcgcttcaag
     7201 aaggtgctgc gcagcctcca agaggtcagg ggaaacaccc cacagatgaa tgttctcata
     7261 agagagatca tcgcggcgct caagtttcag gagctcggtt attcggtgaa cgccattcga
     7321 aagatcaccg gaacgggcat ggccgacata accttgctcg acaaggacgg ccgaaaagtc
     7381 atcgccgagg tcgctttcga gaacctcgca ggcgtcgccc agaaggctat aggaatagcc
     7441 aaagatgata aggccgacat cgccatggcg gtcgcagtgg acctccaggc tcaggagata
     7501 gccattgtcg aagccacatc atttttggag ggcaaagcaa agcctgaggt atataagtat
     7561 gacgagcgcc aggcgttcta cgaagcgacc gatgtgatta ctgttgcgga gatcggggag
     7621 agactcttcc cccatcccat caccgggata gactatggca gaatgtatgc tgcgacgata
     7681 gaatcagaag gagctcaagc agagatcgtt ttcaccaaca atcccttggc cgtcttcaac
     7741 ttcggccaca tagacgggat catcataggc gcagtccacg aacgcgagtc tctgaaaaac
     7801 ctctttctct cgttcggaac gaagactccc atgctcacga tacaggatat tgggccggag
     7861 ccatggggcg taatcggatc gaatgtgtca gacctcgaca aggggatact aaagctccta
     7921 cctgataatt ccgatgacgt gtgcgacaag atcaaggctg cggcttggga gaagactggc
     7981 aaggatattg aagttctgat ctttggagat ggagcctaca aggacccgga tacaggcatc
     8041 tacgaactgg ctgacccgta tccgtccatc ggatgcagca aggggcttcg cagcgcctca
     8101 ctgcggcagg gcacgaaact caaactcctt gtcgaaacga tgttcagaga aggccattcc
     8161 cgcgaggaga tatacaaggc cctcgaggaa cagcaaccgt cccgcgaaac ccttggcaca
     8221 acaccgcgca acatcaccgg gatactcgca accatggccg atcttgccgc cggctctgcc
     8281 gatgccggaa cgccggtggt aatcatacga aactttcctt ttaagcctgc caagtaggca
     8341 ttggaaccta gcagggcggc gtcgggccgc cctgctaggt tttgcgagga ttcacacaac
     8401 aatcgccgca tctccacggc gtgcctgatg tcgttgggag tgagcctatg aggagtctat
     8461 tcaccaccca gacgtgcaaa gaacaccgca tcaagcaaga gcttcccgtc atcttccgaa
     8521 ttcgctacag acgcagccta tacaaccatc aagcgcaaaa cttcgcgttc atgttattat
     8581 cttcttagga aagcgagggc aattacgaag gggagtgaat acttcgtgaa gggatttgct
     8641 atcgacacgt tcgagaagaa ccaagtggtc gatatcaccg cccaggtcga atcgtgcctt
     8701 gccgaccaag ggccacagga cggcatctgt ctagttcaga cgccacacac cacatgtgga
     8761 attatcctca acgaacactt cgattctact gtctccgagg acatactcga ctgtctcaac
     8821 cgcattgccc ccgaggacgc agattactca cataaggaag gcaacgcaac tgcccatatc
     8881 aaagccgctc tgatcggctc gtcagttatc ctcgcgatag aggatagacg ccttgcccta
     8941 ggaacatggc agcgcatcct tctatgtgag ttcgatgggc cgcgccagcg gaaagtgtgg
     9001 gtcaggacat tgaaacaagg cacatgagag tctaagaagg atcaccacgt gtgaggctgc
     9061 ccgcatccgg gactctcgga aacctctatc ccaattaacc acatcatgac cgtatctgaa
     9121 cggagggtgc ccatggctaa tttccgaatt ccagatgtca gacgattgcc agcaatcgcg
     9181 tctatcttcg ttatcttggc cctaatcgga tctgtctgcg ctcgacctct gtctgccgca
     9241 gccgatagtg caataaccgt gaccttcatc gacgtcggcc agggcgacgc gatccttgta
     9301 tccacagacg atggacgcca catgttggtt gacggcggac ccaaggcggc cggagggaag
     9361 ctggtagcga cgctccattc catgggagtc gaacacctcg atgtagtcgt atccacgcat
     9421 ccccatgagg atcatgtggg aggactcata gaagtgctgg actctttcgc agttggcaaa
     9481 gtactggact ccgccaaagc gcacacgtca gccacctacg aagcctacct cagcaagata
     9541 tacgagaaag gcatagcttt ctcggctcca agggcgggag attcatttga cctaggaccc
     9601 gttacaatca aggtccggtg gcccaccgag ccccttccgc aagacataaa cgattcctcc
     9661 ttagtcctgg aagtcgctta cggaaaatcc cgcatcctgc tcacaggcga catcagcggg
     9721 cacgtggagc ggatattggc tgacacagga gtcctaaacc acatccatct gctcaaagtt
     9781 gctcaccatg gcagcagaac gtccacaacc cagacctttc tggcgcagac gtctccaagg
     9841 cttgcagtga tacaggtggg gcagggcaac gactatgggc acccatcaaa ccaggtgctt
     9901 acaaacctga gatcacgcga tgtcctcgca tacagaacag acgagcatgg gtctgtgagc
     9961 gtcaagatga ggccggatgg aaaaacattc tccgtccata ttgaacagcc cggcaatgac
    10021 ctgcgagcgt catcttccag cgatgtgttt caccgaagtt catgccgata cgtgaatacg
    10081 atcgatgcat ccagaattgt catgatctca gaccgcgaac aagcgatgaa tgcaggttac
    10141 aggccgtgca agctctgtaa cccataggaa actcgtggtg aggtgatgtc ggatgaaact
    10201 tgtagccact ctggaccgga tcgagaatgg catcgcaatc cttctggtac gaccggagga
    10261 gtccatacag atcctttggc ccgtctctgc actcccagaa gacgccgttg aaggcgccat
    10321 actcgacatc tcagtggacg tcgacaaagg caagacccag gccgcagaag aacgcgttca
    10381 atcccttctc gacaagctct tgggcaaggg atctcagcaa tcaactgaac cgatatgagc
    10441 gcaacaaaaa aggggcgcac tgcaccaacg cgaatgcctc catacacgtg cgcccatcga
    10501 cctcgcgggg ataagcccta cacgatatgg ccatgcccac cgcagccaca tccgccttta
    10561 gatctttccc caggcctcat gtcatcagca atcttcttca ggccgtcctg aagctgacct
    10621 aaagcagctg acagcgcctg ggcaaactcg ccttcaacct ctattctccg tccacacttg
    10681 ctgcacacga cgtagtcttc gagcagagtc tcaaagctgt cttctataat ctcgacatat
    10741 ggcttgccgc attcgcatga gaatttatgc tcttgttcca ccttgcaaca actcccaaaa
    10801 caatttgagt catcggaacc atttcagcct acgcccacct gcgcgcgaaa agcttgccag
    10861 tcaatcccgg caaaactcca ctcgaggggg tgcggcgaat cacgcttcca gtttactgac
    10921 gcgctccgcc ctcttcatct ccccttctca gccaattcga gggcgattat cgcggcccca
    10981 tatgcccccg caaactgaga catagaagga accgacacct tcaagccaag cttgcgctcc
    11041 aaggaaagcc tcacaccgtc gttgcggcta acaccgccag tcattaccac ttccgtggcc
    11101 agccccactc ggtgcaccat gcccgccaga cggtctgcca cggcgtcaca cagcccccta
    11161 atgatcggct cccgggccac accgtgcgaa agcaagctca caacctccga ttccgcaaaa
    11221 accgtgcatg ttgaggagat catcgccggc ttcctcgttg cgcgccacat cttgccgaaa
    11281 tccgaaaggg gaacttccag cctttcggcc ataacctcga ggaaccggcc ggttccagca
    11341 gcacacttgt cattcataac gaagtcggca accatcccat cctcatcgag gcgtatgacc
    11401 ttggaatcct ggccgcctat atcgacgatt acgtgggcgg cctcggcaaa agcccttccg
    11461 cctctggcat ggcaggtgat ctcagtgacc gtcctatccg ccatcttcac catggaccgc
    11521 ccgtatcccg ttgcaaccat tgctgagata tcctcaatgt caaccacccc ggcatcctgc
    11581 gctgccgctg aaagagattg gaacgcggtc ttttcctggt cagtcccagt ggatatgaca
    11641 ttggttcccg caattctacc atccaaaagc accaccgcct taacagcagt ggagcccaca
    11701 tcgacgcctg ccacgtacac cctttgctac ctcgccttct tcgcgtctat aagctcgaca
    11761 aaggccccaa gacgggtcat cgcctgtgcc tcacttccat tgcgcacatc aaccccatcg
    11821 ccaccaagat tgagaaaggg gattcctctc ctcttcaagt gatctgcgag catagaagcg
    11881 ccgccattcg attgcctgca tccccactga gcaaactgga ccacaccatc aatggagtag
    11941 tcgtcaatca tactatcgac cgcagcgagc cgcctctcta tcggacccca tccgaagttc
    12001 gccaacatct tccgagcaac gctggcgaag gggtcatcag gatcgagctc gctccagtat
    12061 ggccagttgt attcctcaaa ggccactgaa gtttcgagtt ccagctcgag atagtctaaa
    12121 agaccagtct tataatatgg tctgaggttg agccaaagga gcctgtgcct ttcgctgcaa
    12181 cacggatatt cgccgctggc gataattccc cgaatatggc ttgccagatc cttgtagaac
    12241 tctgcaagcc agggattccc ccatgccccg aggaacatga ccacgtagtt tagggcttcc
    12301 cctcccctcc agggagacgg ggaatgctgc cgcagcgcac acacctccaa ataatcgtcg
    12361 agggtcgcat tggacacccg gattgcctcg gccatacctt ccggcgagat ccccggaacc
    12421 ttttccggaa gatcgaacgc aatatgctct acttgatccc gcagacgctt ctgtccccgc
    12481 tcagatcgat cgtatggcac atcaagtaga tagaacggac agccggcgtc cttgctcatg
    12541 cgatgaagag acttcttgcc cccgtcgcaa agatgtgctg tagcaatcac tgcagaggga
    12601 cgcggcagga cgcccaattt cccggcgcca actccggccc tgtggaaact gcacaagtcc
    12661 gcagaatacc acatcgactc ggctgcgccg agagcatcat aggcaaagcc gaaggctgcc
    12721 gcatagcccg cagcaacctc aggaagaaaa ggaaccgcgc caagcccgta cacgagctct
    12781 gtaggcacaa aagccgtcgt atatatgcac ggcctctctc cggtgtaggc ttggcgaaag
    12841 tcatccatca cgcaccgtat agcgcttatc tgataaggct tccagccgcg ccgctccata
    12901 atgtcaacca cctgtttagc cgcgccatag caccatggtt tcctggcaaa tcggcgaaat
    12961 gccctcacct gaagtgaatt gcccacgctc cgcgtgcctc taggccccat ttggctctcc
    13021 cctcccctcc aacatctccc gaaaggccac cgccctggtg agcacctgcc ccgtccttcc
    13081 cgcgccgtat tcgccctcca gaaggaggct gggcaacccg aaactttccc tcagccctcg
    13141 cttcacatcg ggaaaatcgt agtagcccaa atcgcagaac ttcggagcga agtacacaag
    13201 gcctttggcc ccatacatgg ccgcaagcgc gcccaaacgc gcaatacgtt cgtcgcctgc
    13261 gaacattcta ggacatccgg gttttgacag atatgacctc gccaactcca tgtatatatc
    13321 gtagctcggg ttaacatcga cagaaaagtc gtacaccctc tgacacatgc aggaatcgat
    13381 aaagactggc tccagtccgg cttcttcaat gcactctact acggacaaat cgagaagaca
    13441 cgttcccgac acgccgaccc gcacccgtcc agccaacgct gggggttgca ccgagactga
    13501 cagcgccttc tcgatctcgg cgcggaccgc tcgcgttaaa gcgtcacaaa cctctcggcc
    13561 aaacccttct tccctattcc aggtcattcc ccaacgcgcc acatccccca taagctcgtt
    13621 tacgtccata attgcctgca atagccggga agcgcgcaag gtgccttctt ccaaagactt
    13681 tgcagcctcc gccatcgcag ccctcgcctt attcatcgcc tcgcacgcaa ggcgcaagct
    13741 ctgctcctcc acctgccctg ctgggcctac caccccgatg tgcctcgcca tctgccgcag
    13801 ttcgccggca aagtagtcca cagcagcttc atctgctgtt cgcggcacat cgaggtaaaa
    13861 aacgtccgct gcaagacccc agtacctgat tgcatcgtat gcgcgcctca tcgcatcaca
    13921 agaccctgca atggccaaca cgtcaccagg gtcatattcc ctcatcaacg ccctggcata
    13981 tgggcagaaa tcccggggaa gaaggccgtc cgcatctact tcggccccgt ctaggacaaa
    14041 gagcctcttc gccggtagcc cggctgcaag aggcagttcc agcggtatgt agctgcacga
    14101 cgtcaacaac attctcattc ctccggcccg gttcgcacac cctagtacca tcccacacgg
    14161 cggcctctga ttctgctttt tgtcgtgttc tacgcaatgc ccggactttc ctgcctcgat
    14221 gcagccaggt tccaggaacg ctcattcttt ctccctcccc agccggtcaa tgaagcgaag
    14281 caccctgttg tcctctatga ctttgccgaa agacatccgt tccgatgcca acgtaagcca
    14341 cacgagaacg atcagagcgc tcgtccgcca cccgactgtc atctggagaa ggcagtaacc
    14401 aagggctgcg ccgagggcat tggacccagt gtctccaagc attgctgttt cgccaagatc
    14461 cgcagggaaa aaggcgatcg cggctccaag ggcagatccc gccgggctga gcattcgcgc
    14521 caaaccggcc ttcccgtcct tcatccaaaa ccacatgcta agggccgcaa accccagtag
    14581 aaaccctttt attgcgcgcc ctggccgcct atcaagcaag ttgatcaagt tggcagagag
    14641 tgctacgact gcggcgtcca tcaagtccct gattcccacg ggagccattg ccagcagctc
    14701 gacagcgcca agagaaaccg ctccgcccaa gaagaccttg accacccccg tagtgaagga
    14761 gccctgggac aatgcgccga aatgtccgct aaaccccgac tgggcgtgac ctcccagcat
    14821 gtcgtcgata aaaccggcga acccaaatcc gacgatcacc aggaggtagg ctccaactct
    14881 gcccggctcg tccacgtgga tcccgccaag tgctccaacg agaatgggtc ctagaacagc
    14941 tccgaatggc acaacaaggc cgcccgggaa ggctaccggt tttcgacgat agttggccac
    15001 aggtccaacg gttcgcaaga gcatttctgt gacgcccttc aaggacgcgg ccacgatcat
    15061 cccggccaac aacgccccga aggctccgtc ccatatcatc cgtgcctccg aagcaatcac
    15121 ctcccagcgt tcctagggct atctttagcc accgcccaaa cgaatatcac gacgagcacc
    15181 tccgcgctcg tgtcagatgg accgttcact ctgtccgaaa aaatgagaag ctcgccgcgg
    15241 tcgcgccccc acatggcgca aggcgcggcg agccacatgc agagtccccg caagaatggc
    15301 tccggctagc actggaaaac acccacgagc tcgtgggctg atctgtcttg actctaatca
    15361 ggcggggaac gagaatgggc atccaccgac gaaggtgtaa tgggtgcaag agatgcccca
    15421 gaaaaggccg gtgaataaaa gtatccaaac cttggagagc tcactacgcc acgccgacga
    15481 cgcaggcttc ttctggcgcg tccctacacg atgcatccac gcccgctcgg agcatgtcgt
    15541 ctgccacacg ctcagcggcg ccgcatcgtg agcgcctcaa gcacctctac gaattgacgc
    15601 gccctgtgac aaattccagc cagatccttc cctgtctccc tatgagtcat attcgtggga
    15661 acctcaacaa tcgtgtatcc cgcccttgcc caatctatcg ccaagccgac ctcgagtccg
    15721 aaccccctgt ccaacctggg catgtgggcg ccgtcccctg caaacacctc atagaacggc
    15781 tccgccagta gcgcccgctg cccggacaaa ggccacttga gatccaagcc tgccaattgc
    15841 ctgactccgc gctttgcaag cccgactacc aggccaaagc cgcctgaact gatgggctgc
    15901 ggaaatcctg ccaccgccat gtctcccctg ccttcaacca cagcagacac aacggacgcc
    15961 atctcggctg cgctcggtcc cagatccgcg tcggcaaaaa cgagaagcgc cggagccatc
    16021 ttccgcgctc tggcgatccc cgtagctaca gccgcgccct ttcccacagt tttcgccatc
    16081 cggatcaccg tcgccccagc ctcctttgcc ctagcagagg tgcgatcacg agatccatca
    16141 tccacaacga tcacacagtc cgcagcggag cattggagaa gcgcagccac gctctggccc
    16201 acactgggtt cttcgttgag cgcgggcact attgctaccc tcggatgcac taccttccct
    16261 cctgcgcggg cagggccgcc ggtgctggag gctgtccgta taccccgcgg gcacctgcaa
    16321 gcgccagcgc catcgccaca ttgccaggcg cagtcccaac accgtaaacc tcggctgcgg
    16381 gcaacgcctt tccaagctga ctcgagaact tgccggctgc cacgtccgac cggaccttga
    16441 gcacagcagc gcgcgtccat ccaactacca ggggcgttcc tgcactcttt gcggcagcag
    16501 acaaggcttg aagaaaggct gcttcctttg cctcagcagg ccctgacaga gccagcacca
    16561 cgctgaactg gcacctttcg gccatcctga tcttggcgag gccttgggtc gacaaactct
    16621 tgatcctatc catgtcagag gacgtgatag cctgagccca aacactcagc tgtccgctgt
    16681 tggcgcccgc taaagccaca tcggccaact ccacgaccgc cccgactcga gcccccgcac
    16741 cttcgcaaac atccctggca gattccagat cgcctcgatc gacgtctggc atggacacga
    16801 ttcccactgt cttgccgacc aaagacccac ggatcatgta tggcacgctc tcttccgcat
    16861 accttcgcgc ggcgtcaagt tcctgtgcta aggcgtcacg gtcttgtgac aacctgtaga
    16921 agtcggcatc gagcctgtca atcaaggtcc tctgttgcgc cgccaatcct tcaccgccag
    16981 atatggcagc cccaagcaga atccctatcc ctagagcgac gaggaccgcc gccaaagata
    17041 ccacgtgata ccgcagactc atcacggcaa tctccccatc cgatcggctt tcggttcaaa
    17101 gcaggcccaa catcacacgg attttgaggt agatcaacct cgccgtgtca gccacagatc
    17161 cagagaaaaa ggctatagca gctaggggca caaccgctgc caaaaggatc ccgattatgt
    17221 gccctatccg tagccttccc ctgtatagct ggctcactcc ctttgcgtcc accagtaccg
    17281 atccaacctt cagccgcaca agaaacgtgc tgcccatccc aggcctgcct ttctcgagga
    17341 aatcaagcat attggaatga gttcccaccg ccacgatgag ggaagctcct ttctcgtagg
    17401 caaggagcat ggctatatct tcgctcgtgc cgggacagac aaaagtcttg tgcgaaaggc
    17461 cgagattggc gaggcgctcg gcgccggggg atcggccgtc aggatacgaa tgggccacca
    17521 actccgcgcc taagaaaagc gtttcatcgg atactgaatc catgtctcct atgatgatat
    17581 ccggcgcgaa tcccctctct actagggcat cagcgcctcc gtcgacgccg atgaggacag
    17641 gggccatctc cctgatgtag gaagcaacgg cgtcgaggtc ttctctgtat ctagggcccc
    17701 tcaccgcgat caggcagtgt ctgccgtgaa tctgggtctc caacgggggg atgtctatgt
    17761 ccccgaggat tatgtccttc tccttgattg catactgaag agtattgacg acgaatttct
    17821 ccagctcggc atcgagattc tcctcggctt cgatgtaggc ggcctcaagg ctgtcccttg
    17881 tcacccacac cccgacgcct ataggctgcc cctctttgaa aacctcattt ccttctattt
    17941 ccagggcatc tccgtccgca accacgtcca aaagatgagg gccgacccca tccagcaccg
    18001 gaatgcccgc atccagcaga atcccaggac ccaggcacct gtatctgccg gtgatggaac
    18061 ggtcggcatt tatcaccatt ttagggcacg cttcaaccaa ggctcgtccc gacacttcat
    18121 ctatatcggc atgggctatc actgcaattt ccccgggtcg gagccgggaa attagattct
    18181 tcgttttacg gtcagctctg gctgtccctc tgatgcgaac acgggcgttt cccgcggaat
    18241 cctccgctcc aatgctctgt ttgccatcga ccccatcgta ccccatacac ttattgattc
    18301 cccgagctgc gtaggccatt cgccagagtc taggagcaca ataacacaat cagcggatga
    18361 tctgacctgc caggtctatc cttaccttac tggctacccg ggctatgaag gcagaattgg
    18421 ttggacgtcc cttctcagcg tcgacagtat ggccaaatat ccgattaatg aagtcgatgt
    18481 ccccgcgatt ccacgccgtt tcgatcgcat gcctcatggc ccgttcgacc ctggagggtg
    18541 ttgtgccatg agcttcggcg atcgtcgggt agactgagta ggtcatgctt cctgcgccgt
    18601 gccccctctc caacgccatg atgatcgcat ccttcagata aaggtatccc ttcaggtttg
    18661 caggcacgcc cattctatgc agagtcttgc taacctcgac atcgtagatg gaacgttgaa
    18721 ggtccgtgct ctgcgggcat gatgcgcagg attgaagctc ttgagggcgc tccggcggca
    18781 atgctgcgag ccgccggatc cgccgcgcca atacttcaag gtcaaatggt ttcgccatgt
    18841 aataatcggc tcccgccgcc actaccccgg cggcagtggt ctccaggccc atcgcggtga
    18901 ttaccaggat gcgaggatgg gggctcaggg gtgtgctgtt gacatactct atgaccccca
    18961 acccgtcaag gtgcggcatg acgagatcga gcagaattac atcgggcgca acgtccctta
    19021 tgacacttat cgcttcggag ccgttgaaag ctacaccgca catcgacatg tcgggctgag
    19081 tcgaaacgaa gtcggtaaca acttcgacga ggtcgacatt gtcgtcgacc atcaccaacc
    19141 gaaccaggcc ttgggacatt atccgcagcc tccatgaaca ttgatactgc cactttctac
    19201 agcgctgagc ccatctcctg ccttcactga tcaatcgctg gatccactaa accacatctt
    19261 atggcataca gcgcaacctg gatcctgttc tcaaccccga gcttcctgcg tatgcttgtt
    19321 acgtagttct tgacagtctt atcggatatg aagagttttt cacctatggc ctggttgctc
    19381 aatcctagag ccaccattcc tattatctcc cgctcacgag gagaaagttt ctccagcgcg
    19441 agcgcggcag atgagtccag ttctggtcta tcttgcggca caggcctcac ctctcaaccc
    19501 cgtcagggcg tgtacacaga cgaagcaaac ttgcttctcc ctctggcggt acataatatg
    19561 tggtgccaaa tctccctgtg tataggcttt ttgacggggt ctgcgattcg gacccacatc
    19621 gccgcctcct caagcggcca acaagacgat ccaacccgct gcgcgctcgc ttccttccat
    19681 caatccggca acgttcagca tgtgttcggc aaaaaccccg tatcctctcg aagggtcgga
    19741 aacgaagacg tgagtcacgg cccctacgag cttcccatcc tggattatag gggatccgct
    19801 cattccctgt actattccgc ctgtcagctc gaggagacgt gggtcaacca ccctgatgac
    19861 aatacctttc cccgcgatcc gactctgctt tgcaaccttc actatttcga tttcaaacct
    19921 ttctatggaa gacccggtga caacagtgag aatctcggca gggccttcat ggaccgccgc
    19981 agccagcgca accggaatcg gatccgcgta gtagggattg ggaagtggcg catccagtgt
    20041 cccgtatatc cctacttcgg tgttggcctc cacgaccccc acattgtccc gcgcgcccgc
    20101 gaatgtcccg atcttctccc caggtctccc tgcaccactc agatctatcc ctgatacgcg
    20161 cgctgagacg attctcccat ctgtaattgg gatctccttg cctgtctgcg aatctactac
    20221 gacatgtccc aaagccccgt acctcatcgt gcccgggtgc cagaaagtca gggtgcccac
    20281 gcctgccgca ttatctctca cataaacccc aattcgatag ccgccatcgg caggcgccgg
    20341 caagaccgtg acatcgaatt cccgcccatc ccgaacaatt cccatcctca caggcctacc
    20401 ccccaatgca ccttcgctca caagcgtctc cagctggcgc ggctcatgga cctcatatcc
    20461 atcgactgag acgatcatgt ccccgacctc gattcccccg tcgcgggccg gcatgtatct
    20521 gagtccgtct gagccaacaa ccgggtacat tcccatcacc ctcaccccgc ggcttgcgag
    20581 caggatgcct atggagtgtc ccccaggaat cacccgcatc tggggcacta cgtccacaac
    20641 taagcgtttt acggggatcg cattgaagag ccgtacctcc agccgcgttc tccccgcctt
    20701 cgtgggagtc acttccactt tcgatcctat ggcggcatcc atcagaagag agtctggagg
    20761 atcaacgact acggcccctc tgccaagagg gcccatgtcc acactgaatg tttggccttc
    20821 aaaaaccctg atgtaaatgg gagacacata gattctggct accgcagccc cgatggccgc
    20881 aagcgaaagg aaaccgacaa tgaccgcaaa agcgatcgct atgcgtttcg acgaatacgc
    20941 ccgcatcctt ccaccatctc gccgcgggcg tatctgacgc gcgcgtacgc gccggcccgc
    21001 gcgggtttaa cgtgtccggc acgagcaagg cgtattcgcg ctaaaagatg ctgtagttgg
    21061 caactagacc tgatttgaaa tcagactgaa gaagaaaccg attcccgtga cgcttagtgg
    21121 atcaagccac aacggcacaa gagtagttgc gaaatggagc atgcggcagc gggatgcacg
    21181 cccatgaaag gcgatacgaa cgtctgatag aaaaagctaa cacatcgaac aggaatttgc
    21241 gtcctattct caaatatccc gcttcctgta aaacctggat atgaagaatc catcggtgcc
    21301 ggatataccg ggcaggagct caatcatgtg cccgtcgtca gaacagaatg cacgggcagc
    21361 ggctgagggc atgtacgggg ctatcggcga cggcacgaac tcgcgatgac gcaccgcgaa
    21421 ccgctgaacc tggtcccgat tctcccgggt tccaacggtg cacgtgctgt acaccagcac
    21481 ccctccgggc tttacacacc tagcagccgc atcgagtatc tcttcctgca gctccgaaag
    21541 ctccaaaatg tcgtctgggc tcctgcgcca ccgtgcatcg ggtcggcggc ctagcacacc
    21601 caggcctgaa catggcgcat caacaagcac ggcatcagcc ttgcccgagt agatatctgg
    21661 aagctctcgt gcatcgcagc agatcgcctc cactgatggc agaccgagcc tggccacgct
    21721 gtcttctatc atcttcactc gttccaaagc tacatcgcat gccacaacct tcgcaccgca
    21781 tcccgccaac tcagccatgt gggcagcttt tcctccagga gcggcgcacg cgtctattat
    21841 caagggccaa tggccgtctt caatcgcttc ttcggccaca cccgactcat cttccttgcc
    21901 ggattctgcc catagcgcac ctggcttttt cccattcgcg gcacatacga gctccgcaac
    21961 gccaataacg tgccctacca acatagagct ttcgtcctgg acagaaaaga gcccatcgtg
    22021 gtacccgggc aactcctcga ccccatcgat gggacttgag agtataagcg cctcggggca
    22081 taaatggccc ggttcagaga gcacaccttc acttttgagc atgcgggaaa actcttcacg
    22141 ctcgatcttc agccggttga cgcggagcac tatagtcggc gcttcatttc ccgcctccag
    22201 cattgcccga gccacatcaa cgccatattc gcctatccat gactgcacaa gccactcggg
    22261 atatgacagg gagaccgaga gataacctac aggatcacct ttggggtcgg gaaactccgg
    22321 agaaaactct gcagacgtga tcttcctcat aacagcattc acgaaagaag caaggccgcg
    22381 atgacctaac cttgccgcgg actccacgga ttccgagact gcagcatgag gcggaaccct
    22441 cgacatgaag agaagctggt aaacgcctgt gcgcaacaaa gccctagcca gaggatccat
    22501 gagatctaca ggcctggttg acgcatgtcc gatccaccaa tccaaagttt tctttctcct
    22561 tatggatcca taagcgatct ccatggcaag cctgcggtca cgcgcgtcca tctctttccc
    22621 cgaaagcgcc tgtgaccagg cggcattcgc atacatgcct tcctggtcac agaggtacgc
    22681 aacttctaca gcaagatctc tggcagtttt cgaagccgca aactttggcc gtctagtcac
    22741 gctggcttcc tcgcagaatg agcaatctca acaattgagt gatagccgtc gccgacgccg
    22801 caacgtaggt aagagccgca gcttgaagca cctgcctggc cgccggcaat tcatcttggg
    22861 taatatagcc tccgtctgca agaagcgtca gcgctcgctt gcttgcgttg aactccacag
    22921 ggagcgttac cagttgaaag agtaccgccc cggcaaaaag ccatatcccg aggctcataa
    22981 ggaactgtcc ggtcttgtcg tatcccatca gcatgcccag tatgaacagg ggaaaggcca
    23041 tgtgggatcc gaaattggcc accggaagta tcgcctgcct gaaagcaaga ggggcatagc
    23101 cttgcttatc ctgcatagca tggccgcttt cgtgagccgc aacagccaaa gcagctatcg
    23161 acgatccgcg gtacaccgca ggagaaagcc tcaccttttt ggaccttgga tcgaaatggt
    23221 ctccaagagg cgtattgatg ggctcaacgc ccacatcgtg tattccattg ctcctcaaaa
    23281 tggactgcgc gacctggctt ccggtgaagc ccgtcttcga ccttacccgc atatagcggt
    23341 tgaaagttcc ctgcaccttg gcctgagcgt aaattgccag aagcaatgct ggtataacaa
    23401 taatataggt cggatcatag taaaagtaaa atggcaaccc agatcacccc tttcgcatcc
    23461 actctgatgc ctcattcaac acatgccaga gccgaacctg tcgccatggg atatcctgcg
    23521 gcccctggcg aaatccgctg ctggcatctg gcgtttgcca gccgggtgaa ccacatccaa
    23581 ggccatcagt tcgcagccat tcagtccgca aaagacaatg agctctcctt caggtcctat
    23641 tgttagcatg cccggatatc ctgagcaaac cgtttcaccg gcctcgccat ctccaaccaa
    23701 aaccggggca atctcgtgcg cccgcaggat gcgcacggac tgcccggcaa aagaggtgcg
    23761 agccccggga gcgggcgaca atgcccttat gaggttgcct atctcgcggg cgctcttccg
    23821 ccaatctatc tcgaattcat cagacgacag ttttcgggca tacgtagcgg cggaatgatc
    23881 ctgcggaacc cggggcgctt ccccacactc aattagacga agaaatttca caattagttc
    23941 cccgcccaac tccgcaaggg cgcattcaag ccaacctctg tcggcatccg aaggtatggg
    24001 catatcctgg caaatcccga tgtccccagt gtcccagcct tcgtccatat acatgatggt
    24061 gactccggta acctgatcac cgttgatcat tgcccgctct atgggagcag ctcccctgta
    24121 tttgggcaga agcgatggat ggatattgat gaatccatgc cgcggcaccg aaagaagctc
    24181 tgcgctgatc ttttgtccga acgcggctaa caccgcaacg tccggtgcca tctccgcaaa
    24241 gccctccacg aattcaggat cgctcaccct cgccggctgc acaacaggaa gccccaacct
    24301 ctctgcctcc accttcacag gcggagacgc caacttgtag cctcggcccc gaggcctgtc
    24361 aggctgggtc acaacgcata tgatctcatg tcctgcatct gtcagcgcac gaagcgcagg
    24421 aacagcaaac tccggagttc ccataaaaac tattcgcacc tgtcagtctc cctcactgca
    24481 ttgagattcc tcgcactctt cttcgacagg cctaacattc tctgccttgt cgatgtacaa
    24541 caccccatca agatgatcta tctcgtgttg gagaatgcgg gccaaaaagc cctttccacg
    24601 tacagacacg agctttccgt ccctgtcgta ggcaatgacc tctacctcag ccgatcttgt
    24661 gaccgttcca atcattcccg gcacagacaa acagccctca tcgtccaccg cttcgccccg
    24721 tcgccacacg atttcagggt tgaacaacgt gcggggaccg tcacctgcat caatcacgat
    24781 caacctaacg ggaatcccaa cctgcggggc cgcaagcccc actcctggag cgctatacat
    24841 cgtctcgagc atgtcgcggg caaggtcacg catcttgcca tcgattatct tgactggttt
    24901 cgcagtttcc cgaagcaccg ggtctccctc ggtgcggatc tccaaaatca acaatacggc
    24961 ctcccattcc tagctcacag aatacttgat ggatccatat ctacgcttat tgtaacccct
    25021 gccaacctag gctggcaacc tgcggcggca acagcgcgga tggcatcggc atcgcttgaa
    25081 aagagaagaa gatgccacct atatttccct gctatgcgtt gaataggtgc tggtgctggc
    25141 cctataacct caacagctcc cctcgccacc ggttccgtgc ctgtgcacgc cctccttatc
    25201 ttccctgcag ccttggctgt cgcagattca tcttcccctc tcactagtat gcgggccatg
    25261 cgccgcgcag gcgggtaacc catttcccgg cgtgcagcga tttcagcatc gtaaaatgca
    25321 cggtagtcgt gggcagcggc agccatgatg ctataatgct ccgggttgaa tgtctggacc
    25381 actaccagcc cgggttctga tccccttccc gatcttcctg caacctggga tatgagctgg
    25441 aaagtgcgct ccgcggcacg gaaatcggga aggtttaggc tggtgtctgc agagatgacc
    25501 ccaacaaggg tcacattggg gaaatcgagc cctttggcga tcatctgcgt tccgatgaga
    25561 atatcggtcc gtcccgcccg aaacgccccc aaaatcgccc ggtgcgcccc cttccgccta
    25621 gtcgtatcga catccatccg agcaaccctc gcgcggggaa attccttcct taccgcctct
    25681 tctatgcgct cagtcccagt cccagaatac ttcatgttca tcccgccgca cacaggacac
    25741 acgtcgggaa caggctgcga ccagttgcag tagtggcaaa ccatttccga tgcccttgca
    25801 tggtaagtca gggaaacatc gcaattcgga cacgaaatgg cctggccgca ctcgagacac
    25861 atcgcaaaag tggaaaaccc gcgcctgttc agaaagagca ttacttgctg tccggaatcg
    25921 agcgcctcct ccacactact ccgaagaagg ttgctgaata ccgacttgtt tcccgacttc
    25981 atctcccgcc tcatgtctac gacgcggatc tctggaagcg ggcggtgatc tatgcgcgat
    26041 ggcaactcta tccgttcaca ctcgcccaat tcggacaact catacgtttc aagggccgga
    26101 gtagccgaac ccaagacgac agtggcgccg acctgctttg ccctgaaaat cgctacgtcc
    26161 ctggcgttat agcgcgggga gtcgtcttgc ttatatgaag aatcgtgttc ctcgtcgaca
    26221 ataatcatgc ccagcgacgg cactggtgcg aacaccgctg atctggcgcc aactaccaca
    26281 tctgcatctc ctgcacgagc ccgctcccat tcatcaagcc gttccccagc gcccaacgca
    26341 ctgtggagca gagcgaccct gtcgccgaac cgccccatca caacgtcgac catctgcggg
    26401 gtgagcgata tgtcagggac tagcaggatg ccttgctttc ccctgtctat cacgtgctgg
    26461 atcgcgcgaa tatagacctc cgtcttgcca gacccggtca ccccatggag caaaaccacg
    26521 cctcccccgt tctccgcgac atcagttatc ctgtttacgg catgctcctg atcgtcagtg
    26581 agcacgggcg gcaccgattt ctctatttga aggcctgcag tgggatcccg ctgaatcgcc
    26641 acatccattc gttcgagcat cccgtcgcga acgagagcat caacaacaga tgacgaacat
    26701 cctgccctct ccagaagctc tgccctggga atcgccagtc ctttggtccc cgttttgtcg
    26761 atgtcctcgg cgaataccct cataacggct gcccgcgctg gactgcccgg agcaagactt
    26821 aacgccaaag cttgcgggtc tatctcctcg ctggccaaac gaaacgccct tacagtccga
    26881 ggacgaaccc tgggcggcgc ccacatagtg tccgtctcga ccaaaccggc ctttgtaaga
    26941 gcagatatag gtcttgtgat gtctgcaatc tccaatgaag aagctatctc gtctcttcgc
    27001 gcgctcccgc cgcattccac gaggtaatcg aagacttcct tggctgcacc tgaaatcttc
    27061 gccccttccg catcctcatc tgtcgcacac acgcgcacta ttgtggatga ttccagcttc
    27121 gtgcctgacg gcaccaaagc ccgaagcgcc tcgatcaaca gacacatgta ttcatccgcc
    27181 atccatctgg ccacggcgag caattcttcc gggacagggg gcgcactctt gacgcacgca
    27241 gttatcggct tcacctttgc cacggcaggt ttttccgttc tggcaacgac aaagccctca
    27301 acagatctgg gtccgaaggg caccaggacc cttgacccag gcacgatctt gtccattaag
    27361 ttgtcaggaa ttatatagtc gtaacacttg tccaccctgc gggcaggcac gtccacaacc
    27421 acctgagcat atcttccgtc caaggtggcc gtcacctctc atctatgtct ccaaggatct
    27481 ttctttcaag ggttcttttg aatcgggtgt gcggaagctc ccggtcactg aggggcaaga
    27541 ctaatacaga gtatatcccg agcctattcg cgccgcgaat gtcggtaaaa agctggtctc
    27601 caaccatcac cgtagaatca gcgtcagttc caagcttgcg catggcacgc ctgtagcccc
    27661 acgcccacgg cttaacccaa ccccgaacgg cgcccacatc gagttcgcgg ctgaaccaat
    27721 cggcgcgggc gccgtagttg ttggttagga tgcacaggtg gaagcccctg gccttggcct
    27781 cctcaatcca ctcgcgaact ggaccgggaa tgtcgtaact gccccaagga gccaaggtat
    27841 tatcgatatc cagaatgata cctgatattc cccttgcaag aagatcatcc aggtcgattt
    27901 cccatactcc ggatactcga aggtctggtt taagcaggtc atgattcctc ctctcgccat
    27961 agcccattat accatggcaa aacctcattc tgcgatgcgt acgcccacag cccgcacgga
    28021 cccatagacg tttatcgtcg ccccagaatg cgctacaggt gagtgcggcc tcagcgagag
    28081 gagtcgcatg tgctaattgg tggccgggca gccggctcgt tgcggaatct ccatggcaga
    28141 tcaggtgcca atcgagaact ggaataggcc gtatgggtgt cgccgactcg gatgctagct
    28201 gggtgtggac atctctttca tcagtttggc cagagcacgc ttctctatcc tcgacacata
    28261 agatctcgat atccccaatg cgcgcgaaat ctctctctgg gtctgacggc gcccttctcc
    28321 catgccgaat ctgagttcca acactctccg ttcccgtggc gacagcttcg caagcttctc
    28381 cacaagtctc tcctgttcca ccttagactc cacaatatcc gcaacggtgt cgcccccatt
    28441 ggtgagcacg tccatgagga ttatctcatt gccttccttg tcggcgccta ttgggtcata
    28501 tagcgacaat tctccccggt tctttcgtcc tgagcgcaaa tgcataagta tctcgttttc
    28561 aatgcaccgt gctgcgtatg tcgcaagttt cgtcttcttc tgcctatcga aggtgtttat
    28621 cgctttgatc aatccgatgg caccgatgga tatgaggtcg tcagtatctt cgccgaccac
    28681 gtcatacttc ttgactacgt acgcgactag tctcaggttg cgctcaacca gtatgttgcg
    28741 ggcctcttcg tccccggcct ctttcctttc cagcatctgg cgttcttcat cttctgtgag
    28801 cgggggcggg aaagcgccgt ttcccgcaac gtaagaatgg agaagcatga gccctcttgt
    28861 gagaaggtct gcaaaagcaa caagggcgct ggagatcaag acaggccacc tccgtccctc
    28921 aacgctttgt gatacagatt tagcttatga ggaaacgaga gattcggtgc acgtccgagg
    28981 tgccccgctc ctaacaacta ggctagccga aaaacccgag gatttcgtca caaatatctc
    29041 cgaatatggg ggctgcaaga tctgcacctg attccgcccc ttccaccagc accgaaatga
    29101 caactttggg atggtcggct ggcgcgaatc cggcaaacca agcattgtag atgggctgtc
    29161 ctgtctctgt aaccctgcct gtctgggcgg ttcctgtctt gccagccgca ttcagtcccc
    29221 ccagcgcatc ttgcgcggtc ccataggata ccacccctga taacatggag cgaagtttgg
    29281 aggcgacacc ggaatcgagc gcgcgggcag cctcccagtc catcttcatt accctgcctg
    29341 atgcggagtg cactgcctcg agcaccaaag tcggatgacg caagatcccg tcattagcca
    29401 cggcgcacat catcgtcgcg acctccagag gcgtcacctt aacaaccgat tgtcccaggg
    29461 cgaagttggc tagctctgca gcggccatgc gcgcctgtcg agggggagtc tggcctgtgg
    29521 acacatttgg aaggcatgtt gatgcagcaa cctcgtagct ccctattccc agggctctag
    29581 cggcgtccac gataggcccg gggcccacgg caagggctgc atcgacgaac gcggtattgc
    29641 aggagtatgc catggcatcc gcgaatgtga tgtcgccgtg acctccacgt tcgaaagcat
    29701 ggcatctgaa cgtgtgtccg cctgcctgta tcgcccctga atcatggtag acagatcccg
    29761 gcatcctcgc gccttcgttt aatgcggcag ccgcaaccac gaccttgaag attgagccag
    29821 ggtagtaggg ataaacagct ctattgagca atggcgcatc ggcgcgagcc agagagctcg
    29881 aaacgtcgtt ctgatcatac gacgggcggg atgccattgc cataacctgg ccggtccatg
    29941 ggtccataac caccactgcc ccggccagtc caggagcgcg cctgtcgaat gctcgctcta
    30001 ctgcctcctg aacgcgccgg tctatagaga gccttaccca accgggctcc tctcctgcca
    30061 cagccaatcg gactccaagg ccaggtattt ccttgcctct ggaatcgcac atcgcccgaa
    30121 ccacgtccct accctgcccg ccaccaccaa ggaagcgttc gaaggcgagt tctattccct
    30181 ctgcgccaac ctgtgacccc gtaagatagc caactacatg cgaggctagc ccagaccacc
    30241 ggctgggaat actgatgggg acaatgcctg taactgcctt cgccatgccg acaatctcct
    30301 tgcgatcgta tccgggaagc cggataactc gcggggctcc atcaagcacg cccgggatca
    30361 tgcgctcgag cttatccaaa ctgtctcccg gcccgctaat cgattgaggg aatatggcga
    30421 ctgccagagc agcgccgtct tcgcctaaat cgccacccaa tccaatagag gtgccaccag
    30481 caagtagctc catgtttctg tcgaagatcg gccctcttgc gagtcggatc acggcgtcta
    30541 cagacctctg ctccactgct tttgccgcca aaggccggcc tcttaccaat gaaatccagg
    30601 cgaggcgaac cagaagcaat ccaaagcaca caataacgag gccaattagg aaattgagcc
    30661 ttgaagcgtt tcgtctcgtt gcacccaccc cccatcctat cattccccca aaaccttcag
    30721 gaagcatcca tcacggctca gaggagagga tggtgaagag gctcgatgac tggccaaaag
    30781 gagttgggct cggtccctaa gctctcggag tggtcagatg ttctttgaga cttgcccaga
    30841 ctgatcaatc tgggcaaaag cccgtcaatc tgaactgatt gccgatttga gcctcgcgat
    30901 aagcaactct attgccactt gattgaaccc gccttcagga atgatgaagt ccgcatatct
    30961 cttattgggc tccacgaact gcaggtgcat aggacgaact acatctaggt attgctccac
    31021 cactgaatcc accgttctgc cccgctcctt gatatcgcgc atcaatcgtc tgatgaatct
    31081 cacatcgggg tcggcgtcca cataagcctt gatatccatt agggcgcgca accgagggtc
    31141 ttctagtatg agaatgcctt caagcactat gacgggcgca ggatcgactc gctcagtctg
    31201 tggggtgcgg gtataggatt caaaggaata cacgggtttt tctaccggtt tgccctctat
    31261 gagagtttcg atgtgtgata tgtaaagatc ggtatcgaac gcgaacggat ggtcataatt
    31321 ggtcttcaac ctctcttcga agctgaggtg gctctggtca cgatagtagg catcgtgttc
    31381 aaggactgct acggcatccc caaaagcttg cgagactgat ctgaccactg tggttttacc
    31441 tgaacctgat cctcccgcga tccccaccac tagtacttca tctgacattt ggaattcttg
    31501 caccgccttg tgttgcccct gtcatcgcga cgaccgcgag tctctctcga ttcttgcctt
    31561 cgcccggaga tgctcgctgt atgtcttggt gaatacgtgg ctgccatcgc ttgcagcaac
    31621 aaaatagagg taatcgccgg gctcgggatt gagcacggcc tcaatagatg ccaggcctgg
    31681 attgcagatc gggccgggcg gaagtcccat attaatgtag gtgttgtagg gcgaatcaat
    31741 ctccaaatcc gggttgagga ggtgctcttt cgggacggga agagcatact ggaccgtggc
    31801 gcagctctgc aacttcatct gtttctggag acggttaaga aacacccgcg ctataatcgg
    31861 cctctcttcc gggtgctttg cttcccgctc aacgatggac gccaaaatca acgtttccct
    31921 gtccgtcagg gacatgggac gtgatggatg atggtcaaac gcagcctgcg cagtatgttg
    31981 gaatcttctc aacatggttc gaacgatgcg gagagcgccc actccattgg caaactcgta
    32041 agtatcaggg aaaagatatc cttccaacga tgattggtcg gctacaaacg gaaattcctc
    32101 ggctaggtcg tgcggaggat tgttaaccag agcaaggagc tcttccctgt cagcaagtcc
    32161 gcgctcgtgg aaaatatcgg ctatctgcaa cgcggtcagg ccttccggta tcgttactct
    32221 gactgtgact actcttccgc tagctatggc atctagaatg ctggcaacgc tttcgccctg
    32281 tatgaactca tattccccgg ctttgagcga cgagtcctta ccagaaaacc gagccatggc
    32341 atcaaacatg acgctcgaac ggatgagtcc gtcttcttta aggattgcgg cgatcttaga
    32401 cgctgacgca ccgtccggca aaacgactct gactcgaact tcagcaccct cttcaggcga
    32461 aacacccctg gacgcccata tcgcccacgc gcctgctgtc acgagtaaag ccataagaaa
    32521 aaccaacggc cacgccaacc gcctacgggc tctttctctg gtctgaacag tacccggcat
    32581 tctgaaggcc ccccgtcccc tattatgtgc acccctggga ccaacaataa ttatagggtc
    32641 aaccgcgttg ttgtgcaagg aagctcggca tgatccgcaa caatatggcg actgattcct
    32701 ccgacttcaa tgggggcctt ttcagacaac cgaaaggtga ggcagattgg atgtctccga
    32761 aacggccttt tgcggcattt cgcatgcagg atacagtccc acgcaaaaca gcgacgaaac
    32821 gctgaaaaaa tggtgacgcc aggccgatta cctggcgtca ccatacttat tgctttcggc
    32881 tgccccaatt gcgcccgcgc tagaggcggc tacatgtctt cgtcgtcatc ctcaccgtag
    32941 tcatcatcaa gaaaatcgtc gtcgaaggct tccaaggctt cgaggaccct ctcatactcc
    33001 tcatcgtctt caatgtctgt gaagtactct tctccatcgt catcaacttc gattcggaat
    33061 ataaaggcct cctcgcagac atcctcgtcc tccagctcgt ccaggcactc tgccggcacg
    33121 agtatagcgt accgcgtctc gtccacctct acaatgtcat cgattgcgaa atcctgctcc
    33181 tcaccattct catcaactag cgtgacgatg ctcacctcgt ctggcatctc gatcactcca
    33241 atctagccct attttgttcg ccaagacagt actctaccca cacaaggaca actcgttcta
    33301 tccgcgattc ctgtttttcc tgtcctccag gtcaatgccg ccggaactgt ccggcgggcg
    33361 cagggctgac tcccaactta gtcctcaatg ccaagccagt cgagataacc ctggaggata
    33421 atcgcggcgg cagttttgtc aacggtctgc cgtcggcgtc tccgcgaaag atcggcctcg
    33481 atcagcagtc tagatgcctc tactgtgctc aaacgctcat cccacgtgac cacaggaagc
    33541 ttcaccgccc tctccagtcg ctcacagaaa tcttcaaccc gctgcgccgc cggtccgtag
    33601 cttccatcca tgttcctcgg catgcccaca actatttcac atgccccgac ctttttggcg
    33661 agttctccta tgcggataat atcctgctcc caaccgcgac tttcaattac agtatgaggc
    33721 atggctgcaa tgcctaattc gtcagagact gccactccta ttcgagattc accgagatcg
    33781 atcccgatac gaaccaactt gcaacctcct ataccacctt tatgcagaac tccttgcaat
    33841 gggcgacaca gtacccgcag agcacgcact tttcgggatc gaccacggcc cccgttcagc
    33901 gcccattctg agggctccat ggctgcatgc atctatacat gcgccgcatt tggcgcacca
    33961 catttctatg aggagccgct tttctcctgc aaggccacta aacatttctt gagagacttt
    34021 gcgtccggaa gcgatggcca gctcagcctc gagttctccc acgtttttga tcccgatcgc
    34081 caccaagtct atgtagtcgt gattcataac ccacgtaaac gcgtgtagga cctctctgta
    34141 aagatgtccg cctccaagag gtttcatgct gtatgttccc acaccagaaa ggctcgcagc
    34201 ttctatggcc ctctccatgt cacagacgct cccgtccatg atgcctatgc cttgcaagtt
    34261 gaggatgggg tgcaccacgt cgatctccgg catctccgaa acagcgtcca ccactttcac
    34321 tgcatgccta gagaccgata ctgccctgat caggccattt cccttcgcgt cttaaagata
    34381 ccggatcgcg tcaacatgcc cattgagcgt cagccggctt tcctgttcgt gaagaccaaa
    34441 tatgtcgata tagtctactc cgatatcccg caatgcccgc tccaggcttt cgcgcatgcc
    34501 ttcgtacgta tactgatagc atctcgatga gattactata gcctcccgcg gcactcggtg
    34561 caagactctg cgcaaatatt catagacacc atagagctcg gcagtgtcga agaaattgat
    34621 gccacgctcc acggcctcgc aaatgagggc tgcgcctacg tctgggctga tattagcctg
    34681 caatggcccc attgtcaatg ttccaaagca tagccctaga tacctctatg ccagttcgac
    34741 caaggattcc tctttccatg ttcaggcccc ttccgctagg atctcgtcta caacgtagcc
    34801 cagcagcgca aacgcaggca tcatagccac ggtagcgcac gccacaaccc cggaatcatt
    34861 cacagccatg gccacaagcg cgccagccaa tccgcccatg actgatacgt acacgattgg
    34921 gtaatcatcg cggaatcgtc gaaacaagcc aaaaggcttg cctgcgagca ccgtaagggc
    34981 gatgaggaag gccaacagta cccgtgtcca ggctgtatat cgtagcaggc gcatattcat
    35041 ttccagcttt cgccatgcaa ttacgcccag ctggcctagg cccatgtcaa tcgcttctgt
    35101 cgcaccatgg gcatcctcgc gttccggcac cgctccaaca tctgcatcaa ctccatctgc
    35161 gcctgtttga tcggtcagaa gccgccacgc tctgccaatg tgggacgaac gtttcccttt
    35221 ggcatcagcc caggccatac ctgcgcaagc cccgaccgcc acaacgacta tggcgaccat
    35281 cgctacgacc tttgcccggc gtgaaagagt gcttcttgca aacgccatca cgcccatgaa
    35341 aaccgctatt gcagcggcgg tgaccattcc tccgaagttg gcgcctatcg tcggatgccc
    35401 gagcagcatg gagatggccc ctacgccgat agctgcgccc gccgttgcct tacccgacct
    35461 ggagacaacg cgttccgctc cggtccacat ttcgcccagt gcggaaaggc aaatagcagc
    35521 tgagcccgcc agaacaccca tgagttcgtt gcctatccca tagtaccgcg cccccactat
    35581 tgcggaatgg cccagaaggg acgttgcagc aagggtgcct ccggcaagac catctgcgat
    35641 gatcactacg gccgatgccg cagcgatccc aacagatgcc cacatgcatg atgaagttgt
    35701 tctaatgagc aacaaagaga tgatggccga tcctaaagat gcaaacagga gagtaccagc
    35761 gatcccctga atcctcagga tcggggcaac cagaatgagc aatggctggg ccgccactga
    35821 cagtaacaaa aacttcgcaa agaacacaaa aacagcaggt acccgaagtc cgaggagagc
    35881 aactgcgagg acactgaaaa ccgcaattac aaccgttccg ataaacacgc taagtaccgg
    35941 aacccgcgac gaagccgcaa tctgcagcga ttctgccatg cgcatcagtc tctctgcaac
    36001 attttgttcc tctccagccc tgatgccgat cattggcgat ccatcatgag gccatgcctc
    36061 ctgaacaccc agaccgtcga ggattgtggc ggccagatcc agattggcaa caagtccctc
    36121 gcgacgagtt gtgtcgcttg acagcatacc ccggggatac tgccaacctg cgatcgccac
    36181 aggcccgagg gacgacggtt ctcccggaga gaacagtatg tatgcgaggt catcagaggg
    36241 cggtcccaca gcctcaaaga gggctctgaa aatctcatcg agttttccgt agcatttccc
    36301 aagctgctcg acgcgggcct ccgaggagag gtttgatagg tagcgcccga gccgattcat
    36361 gtcaccgaaa tccacagtca cggcagcgat attcggccga aaaagggcat ccgctgtcgc
    36421 atgcgccatg gccaaagcgt cgcaggaaat tccgaatgga gcgctagggt cggcttcgac
    36481 gatttctgcg ccgaccgcgc cgcaagcaac gcgtcccctt gcatccatga ccagaagaac
    36541 tccaggccgc acctgaaccc cggctacgtc ggaattcccg accgctgccg ccgcaaaccc
    36601 cgccaaacgg agggattcgc caatcagccc gggcctaccc cgataaggcg actgcttacc
    36661 catggcctcc agctccgggt atcccaggca gaggatctcc ccagaaaagc gcttcgcctc
    36721 cgcgcctgtg cgacgcagat agacatcttc agccctttca ccgttgatac gctcatctct
    36781 gcccagccca tagccagccg actcgcgggc gctcattcgg cgtccggcgc cgaaggtagc
    36841 tgctgccgaa aggagcctgt cacccccgtc tgccgttcgg cagttggaca tccctatgga
    36901 tgccgagtca aggaaggaca gaagccaagg gccaccatgc tccagggcat ccctcaatga
    36961 gaagccatcg gctattataa ctatgacacc gcgtcgaccc gggaaatctt cgaacctctc
    37021 cccgttcggc gccgctatgg cggacgccaa ggcatggttt gacactgcgc acaacaacgc
    37081 gacaaggata acaacgcagg ttccagtttt ggagacggaa aaaggcgccc agcgtggggg
    37141 cgccactcga tcattctttc ctgagataac tccgaaccag ctcctctata atctcatcac
    37201 gctccacctt gcgaatggtg ttgcgcgcgt ttctgtgact cgtaatatat gtcggatcgc
    37261 ccgaaaggag gtaacccacg agctggttca cggggttgta ccccttttcc tcaagcgcaa
    37321 tcgtcacttc ggtaagggct ttctttaccg gagtgacttc ctgttccgcc tcgacagcag
    37381 ggtacttcac tgtttcatcc catttaccac tcataaacca caccaccccg ggcagattta
    37441 gtctttcctc atcaggacgc agaatgcgcc cgaaccacat cgacgccggc ttcgatcgcc
    37501 gcctcaagct tagagacatc tcttcctcca gcttgggcca tatcgggcct gccacctccg
    37561 cctccaccag ctactttggc cgcttcgcga actatcgagc ccgcgttaaa gccttgtttc
    37621 acaagatcgc ggctgaccgt cgcaacgaaa agaaccttct cttccgcctt agatcccaac
    37681 agtacaatgc cggaacctag cttgtctcgc acagcgtctg ccagattgcg caaagcttcc
    37741 ggatcggcca gttcgacagg ggcagctaca acaggaatgt catcgaccat gattctgccg
    37801 gcaaccaacg aatccacgcc gccaacggcc aggcgctcac ggaggcgcgc gatctcccgc
    37861 tccttctcct tcaactcgtc caagagcttc tccacgcgct cgaccatcat atcctcagga
    37921 accttaagaa tctcagccaa gctctgaaac tctctatcca tatcgttgaa acgctggagg
    37981 aacgcgtaac cggttatcgc ttctattctg cgaactcctg ccgcaatacc gctttctgac
    38041 acgattttga cgaggccgat ctctcctgtt cgggacacat gcgtcccgcc gcacaattcc
    38101 agactgatat cgccgatacc caccatccgg acttcatcgc catatttctc atcaaagagt
    38161 gctattgcac caagcctatg ggcctcgtcg atagtagtca tcctcacgtc aacaggaatg
    38221 tccgccatga cccatcgatt tgccaaaagc tcgacttggg caagctcgtc tacggtgagt
    38281 gcctcaaaat gcgaaaagtc aaacctgagt cgctcgccgt ccacgtatga accggcctgc
    38341 tgcacgtgtt ctcccaggac ttgtctgagc gcagcctgga taagatgtgt ggccgtgtgg
    38401 tttcgcatgg tggcggctct gcgatcttca tcaacatacg caatgactct atcgcctacc
    38461 ttcagcgtgc cccttatcat atgaacctgg tgggtaatga cgctttcgaa cggtcggctg
    38521 acgctgcaca cctccgccac tgcacccctt gacgagacta tcaatcccgt atcagcctgc
    38581 tgtccaccac cggtcgggta gaacggggtc ttgttcatca ctgtctcgcc atcttggtcc
    38641 tcggagagct ccgcaaggct gttcccatct tttccgatta tggcaatgat attggccgtg
    38701 tccgcaaact catggtaccc cacaaattcc gttgtcaaat ctccgattgc gtcatgatac
    38761 ttcgcgctct tctcatcaag gtaggcattc tcgccccgag cggcacgcgc ccgctctctc
    38821 tgagcctcca ttgcctcatc gaagtgggcc ttgtcaactt ggaggccacg ctctgatgct
    38881 atctcctcgg tcagttcgaa cggaaaaccg tacgtgtcat aaagtttgaa agcatcctca
    38941 ccggggagca ttctaccccc atcccgttcg acctcagcaa taatcgattc caaaactaca
    39001 gacccttgtt caagcgctgc agcaaaccta tcttcctcga gagctatggc cttcagtatg
    39061 cgatcgcgat cgcgtgcaag ctccggatat ggggcggcca ttacatctat gaccgtatca
    39121 gcaacttgtg gaaggaagga tccctcgatt ccaacaagtc ttccatatct ggcagcccgt
    39181 ctgagaaggc ggcgcagaac atatccacgt ccctcgtttg tcgggaaaat cccatccgct
    39241 accatgaaag cggcggcacg aacgtggtca gtaatcaccc tgatcgccac gtcactctgg
    39301 tggtcggcac catattctac acaggccagc tgagccgctc gatctcgcac cacgcgagtc
    39361 tcatcggtgt cgaaaatcgt ctcagccccc tgaagcacgg ccgcgaccct ttcaagtccc
    39421 attccagtgt cgattcccgg ccgctcaagt ggagtatagt ttccatcttc gtctttatgg
    39481 tattggatga agacgaggtt ccacagttca atgaatctgc cacagtcgca gtcaagaccg
    39541 cattcaggag atccgcaccc cacatcggcg ccacggtcaa tgtatatttc ggaacagggg
    39601 ccacatggcc caacccctat ctcccagaag ttatccttct tgcctttcct gataatgcga
    39661 tctgcagaaa cacctaccac atcgcgccag atattaaagg cttcgtcatc atcgaggtat
    39721 atggttaccc aaaggttttc aggggctaaa cccaagacat ccgtgacgaa ctcccacgcc
    39781 cacggtattg cctcggcttt gaagtagtca ccaaatgaga agttgcccag catctcaaag
    39841 aaagtgccgt gacgggtagt cttccccaca ttgtctatgt cgccggttcg cacacacttc
    39901 tggcacgttg tgacccgtgt gtgctcgggc actgcagtgc ccagaaaata cggcttaaac
    39961 ggcaccatcc ccgcggctgt taggagcagg gtcggatcgc ccgccggaac gagcggcgct
    40021 gacggcagaa ttttgtgccc ttttgattca aaaaacctga gatacgcttg ccttagttca
    40081 tcggttttca catattacac cccatctaga aagcccctgc cgcatagccg ggatgcttct
    40141 gcaaactctc gtaagtctac tcaagtacat tatcataacg ccgctgctcg cacaatacgg
    40201 catacggctc cgctcccatc ctccacatgc acaggcttcg cgctatgaat aactgaaatg
    40261 gccactgtgc catggcagat ttcaggcttc ctcatgcgtt aggcatacgg aatccaatct
    40321 ccacaggacg aagctggatt tccacttcaa atcgccaaaa acgattcata aaccactccg
    40381 gtacgaagaa gaccacttta cccgcaaaac gccaggccca ccacgtcaac ggctgcccaa
    40441 aaggctaaca attcaagccg aaacgaggtg ggcccctttc tcgggaaagg cgcgctgcta
    40501 cagaatctcc cagtcgatct caatgtctac cgctttctcc aacatcacat cgacttgaca
    40561 gtatttcaac ctggcaaggt taaccgccca ttctatgtcc ccatcggtga caccgctctc
    40621 ctcaaacctg agcgaagcat ggattttggt gaacacagtc ggtggctggg gttcccgttc
    40681 agcctcgatt gtcatggaca tcttccccac atcaagcctc ttcttctcga gcaaggattt
    40741 tacggtcata gagagacaac caccgagaga aaacaagacc atttccatgg gagtagcggc
    40801 agcggcgctc ttcccttcct tattcgaatc catgacaacc cagtgattcg tgtcagactt
    40861 ggcaagcata gtaaggtccg caacattgat tatctctgct ttcatcatct gttcctccct
    40921 gcgcatcatg caactgaatc atcacaatcg tcaggcattc agtccaagcc cttctattct
    40981 acctgttgcc cttcgtatcc ttcgcgccct gaccgggtta aggtccgaat ccttgcgaga
    41041 cgggtaagct caatctgttg tcaacaggag cctttctccc agtatgaata tgacagcggc
    41101 tactggcact gcaaaaagta tccccattac gccaaaaagg gcacctccca cgagcagcgc
    41161 acctataacg gccaggggat gcaaacctac acaatcgccc acaatcctag gggagactat
    41221 cgccgactcc acctgttgga tcaatgtgaa tgccactgtc actatgatcg ctgtgagggt
    41281 agatttctga acagccatga gaacagccgg taccatgcca aggagcgggc cgaagtaagg
    41341 tattatctca agaatcccaa ccagaacccc tatgggcagc gcatattcga cgccaagcaa
    41401 ggccagtgaa ccccctgtgg ccacgcccac aaaggtgcag acgattacct gcccccttac
    41461 ccaaccagcc aatgcttcat ctatcccgga gatggcaccc attatcctgt cgcgtgaatc
    41521 ttggggaagc caagatgcga aacgttcttt gatgtcatcc acgtcccgag tgagatagaa
    41581 agcgatcaca ggagcaacta tgatgcttcc cagctgcgaa atgaggatgg atacactgcg
    41641 ccttgcagcc aaggcaaact cccgaatata tgactcgacc ccggccacag cggcttccgc
    41701 tatgcgtgaa gtggttcgct caggcaggaa tgtgaccttc tgtccccatt catgaaccaa
    41761 cgtctccaag cctttgccgg ttcgccattc tgccttcgaa ccttccttat aatccggagt
    41821 tggttcctgt aagctcgtgt ctgatccacc ggagtccatt ccgaaaccgc tcgcgtccga
    41881 aaaaaccctc acaatctcgt ccagttgcgc cgccagaggc ggaataacga atatcagcga
    41941 gtaccctatt acagcagtca ccaccaaata agccgtgaag atagctgcag atctttgggc
    42001 tccccgcgcc tcaataatca tcacaagcgg tcttatgaga taagatatgg caattgcgat
    42061 cagaaacggc acgaaggcgc gtcgcgcctt gtaggcaacg aacaaggtcg ccactaccgc
    42121 agtcataatt gctatggcgc gcactatcgc cgcgccccag cgcgatgcca catccacatc
    42181 cacatccctc acagtccacg aggagaaact gattatatgc aggtgcgcac cgactctacg
    42241 ccgcaccaag aactcctaca gacggcaaat agaccggcca cggcaggcaa cgcccgtgac
    42301 cggtcctccc tgaccatcgc tggctagctc tcacagtcac ctgtgacgcg ccgccctaca
    42361 gactgcatcg cctccactac tggctcggcc atagtccgaa cttgtctgga aactctagta
    42421 cgcctcatcc ccaacggagt ttcactcgca acttcctcgc gcaatgtctc cctgccagac
    42481 cgagatgagt tcagcgccat caaaatccca acacccgccc caataaggct gcctgtgagg
    42541 aagatcttcc aggaccttcg aatcttcggt ccctcccttc tgcaccacgt attattccct
    42601 ccgaaggagg agagtacccg cggcgcagcc agattcgccg gctcgggacc gaccatgtcc
    42661 aggcaattgc ataccacacg tcactttatc gaaaatgcca ttattcctgc ccttcgacct
    42721 cgtcatggtc atgttcgtca attcgtatgg atgcaggatc gatccctaag tcaagatctt
    42781 tatgtctctc aaggtagtcc ttgatagccg catgcaccgc gcttgccgcg aggttggaac
    42841 aatgcatctt ctgcggaggc aatccatcga gagcctcggc cacagcccgg ttggaaacct
    42901 caagggcctc agctaaggtc ttacccttga ccatctccgt agccatgcta gacgtggcaa
    42961 tggccgcccc gcatccaaag gtcttgaact ttatatcagt tatgattccg tcatctacct
    43021 tgatctcgat cctcataatg tccccgcaaa ccggatttcc tacctgtcca actccatcgg
    43081 ggttgggcat ctcgcccacg ttccgcggat tgctgaaatg atccatcacc ttgtcgctgt
    43141 acacttagtg cacctcccca aagctgcggg gacagccccg ccgaactcaa accatatcta
    43201 gcgtttttcc gccacatcgc aaccatgggc gttagcacag aattccttga tcccacactc
    43261 cgtcttcaca taaataggcg acattgcgcg aagcttctcc acgatgcgag gcatcacatc
    43321 gagcaccctt tcgacgtcgg catcctcggt gtagcgccca agggtcatcc tgactgaacc
    43381 atgggcaact tcgtgaggta tccccatggc catcaaaacg tgcgatggag caagagaacc
    43441 cgatgtgcac gccgatccac ttgatgcggc aataccctcc atgtcgaggt tgagtaagag
    43501 cgattcacct tctacgtaga gaaagctcat gttcacgttg ttaggtagcc tcaatctgcg
    43561 atggccattg agtctcacat cgggaatgcg ctgcgtgaga ccttctataa gcttgtcgcg
    43621 gagcctctcc tccttggccg acacctcatc catttcgctg acggcaagct cgatggccat
    43681 gccgatcccg acgattccag ccatgttttc agtccccgca cggcgcttgc gttcctgtgc
    43741 tcctccgtcg attagaggtg ccaacctcac gcccctcctt gcgtagagag cgcccactcc
    43801 cttgggcccg taaaatttgt gtccggaaag ggaaagcata tccacgccga gggactttac
    43861 gtccaccggc atgtggccga cagcctggac cgcatcagtg tggaacaaga ccccggcctc
    43921 ccgcgcaatc cgcgagatct cctctatggg ctggatcgtg cccacctcgt tgttggcata
    43981 cattatgctg ataaggcagg tttcaggggt tatcgcgcgc ttcacgtctt cagggtcgac
    44041 catgccctcg gagtcaaccg gaagtatggt cactgaaaaa cccagtctct tgaggctttc
    44101 gacagaatgc agcactgcat gatgctccac agccgaggtt atgatgtggg acttctcgcc
    44161 gtacatggcg agagccgtgc cctttatggc gaggttatca gcctcagatc cgccagccgt
    44221 gaagatgatt tcctccggag cggcgccgat cgctgccgcc acctgctccc tggcacggtt
    44281 cagatcagcc ctggcctcgc gcccgtatga gtgtatactc gaagggtttc catacttgtc
    44341 ggtaaggtag gggaccatgg cctcgaaaac ctcgcgcctt accggggtcg tggcggcatt
    44401 gtcaaggtaa atcctattca tcaggccttt cctcctcttt gttgccccca atgtctttcg
    44461 ccgaatccct caggtctccg gcgatcatgt cctgcagcgt agttgaatcc agaatcgatg
    44521 ttattccatc ctgaagcctc acccacagcg gatgcgctgc gcattctccc atcctgtcgc
    44581 aaatagcctc gccctcttcg tccgcgaggc aatcgcacag agctatggga ccctcgagag
    44641 cccggactat atcgcctact gttatgtgct tcggtgcctt tgccagagtg tatccgcctt
    44701 gggctcctcg ttgactgtag atcaagcctt ccttgcgaag agccgcaaaa agctgttcaa
    44761 gatatttctc tgatatgccc tgtttctcgg ctacctctct cagcgccacg ggaccgctcc
    44821 cgtggacctc ggcaagctcc accatggccc gaactccgta ttcagtgcgc gaactaagct
    44881 tcatcgaatg tcacgctacg gaggcccccg ctctcaggtg cggggggaag tagcgttttc
    44941 ccctcctttc gcacaggatt ggccgatgcc gagatccatg tgccatccaa cttgttgcgc
    45001 aggtcaaaac agcctgtgtt acgtcttccg aaaacaaagc cctcctcata ctcagcctta
    45061 tcaaatgaag gcgcctatat atttagtcaa tcaatccaag tcaatccccg cttttgggct
    45121 tggggtggtc gaccgtatca cccccgtcac cgcctgacat agcctgcaga ttcgaccgcc
    45181 accgaatctt gcgtgctgtt cacatgtgaa cactgcttct gcctggttcg gcataatccc
    45241 tactgaatca gtagggattt gctactgaca gtatacgccc gcatagtata tgtgtcaata
    45301 gccaacggcc ttgtttttct gaacttgccc acaacatcaa cccaagacct caccaaaaac
    45361 aaacattgag cgtcttcctc attttgaccc attgtccacc aggaacgggg ctgaatcgaa
    45421 tcacatcaac ttgcttagtg caacgctcga agtaactata gagtcgacac atcgtcccat
    45481 tgctgaccaa gcctgcacgc tcagcagctt tgcaagacac cataccctgt ttggacattg
    45541 accggaatgg ctgtccttga tatgctcaac ttggcagcta tgccatacgc ttatatcttc
    45601 gagagtcgca caaacgccaa gctatcggct cagccaggcg tctgtgcgca ggcatgcatc
    45661 gccatgcaca cgccaacaca agcaagacgg gaggaatgtg gcatgaaacg ttctaaacga
    45721 ctagtcattc aaaggctggc actttcggca atattgctca tctttgccgc tacaggacca
    45781 gcccattggg gctgtccaag cttttgcctg gcagaagcgt cgtcggccac tgccaacgac
    45841 gacgccacaa tcgtcggctt ggccgatctt cgcaaatgcg atgaacaggg ggtacccctt
    45901 ctcatcgacc aagtagtcac ggttgaagga gcagcgctta tcgatacagg caagtggcac
    45961 aacgccgcga actacttcgc gatcgtaggg ccagctcaac ccgtatcgct ctcagattgg
    46021 gccaaagctg tcacaccatc tgatcgcgtt ttcggcacgc tcgtgtatct ccccggaacc
    46081 accgttcccc aggtaaaagc gggcgatttt gttcgtgtta caggaaaggt gtctatcaaa
    46141 ggatacacca cagattatgg caccacggta attgtgccct ccagttccga gcagatagtc
    46201 atactcgaac acggctccga catcataggg aatctgccgg caactcggct cgccaccgat
    46261 ccaaccttcg ctgaagccga acccctcgaa ggccagctcg ttaccgtcga aggaaggctc
    46321 ttcggctatg atgacgaggg aatcacccaa ggcttttggc tcgacggaag ccgcgacgga
    46381 aacatagagg acgccgaggg catgatgcag gtcaaattct acaactatca tggtctcgac
    46441 atatcccatc ttggaaacgg ctcctacgca aaagtaacag gcgtgctcgt tcaggggcag
    46501 gatttcccgc cataccacgg cggctactac atccgcccca ctgctcagga attcatcacc
    46561 gacgatccca gtaggaagct ggtctcggcg gaatcagaaa tgagagccgc aaagatcagg
    46621 tacaatgttg gcgaccctgt ccacatgtca aaaaaaggca tctttcaact gagtaccatg
    46681 gtctctagca cctcataccc attcgtagac cgagttcatt ctcactggaa tccagatggc
    46741 agcggcgtcg tgttcaccat gggaaaactg aagagtgctc ggagcactct ggagaataca
    46801 gagacaaatc tatttctcat cgattttcgg ggcgggaatg agcattccca gctcaccttc
    46861 gacaaaagcc ccaaggaatt cccgcgatgg tctcctgacg gaagcagaat agcctatagc
    46921 gcgttagaaa gtcccgacga actgtttggc aagtgggaca tttggatatc gaacgcgtca
    46981 gacaattcag atcccataaa agtgactgat gggctatgct atgatacgta tccaagctgg
    47041 tcgcctgatg gaaagagcct cgtattccag tccgaccgca gcggcaactg ggacatatgg
    47101 cgaatagacc tcgacggcag tgtcaatgat ggtagcgcaa tccgtctcac ccgatcaatg
    47161 tcggatgatg gctgccctga ctggtcccct gatggttcaa ggattgcgtt ccaatcagac
    47221 aggaatggca acagattcga catatggatc atgtctcttg agagtccgga gaatcacgag
    47281 agtcctgaga agaacgcttt ctgcctgacc ggatcgctct cgggcgactc tgtttgccca
    47341 cgctggtcca atgacggcac tcaaatagca ttcatgtcaa atcacttcgg aacatgggac
    47401 atatggatgg ccgacgtagc cacaggggat ctgtggcgcg tgactgatct tgagggcgct
    47461 gagaagtatc cctcattctc ccacgacgga cgacgcatag tattcgccgc tgatctggga
    47521 caaggctgga acctttacgc agccgatctc aagagggcca agccggccaa attcacagga
    47581 tccgagggcc aggcaataac gcctccccag gctccgcctg agcagccaaa ggcaaagccg
    47641 agaggggaat atggaatagt ctctccccag cttgccctgc ccgccttcgt aaagccggaa
    47701 gggacgctct ccatagaagt gatttctccg gttcaagata gtccgacatc cgccactagc
    47761 gtggaatgga aaatacggct tttccccaag atcggttcgt ctgaggcttc cgcaatctct
    47821 ctcaccccac acaaggccat agccttgccg gatggcaggt ggagcatgga ggcaacaatc
    47881 cctgaggata cgccgatcaa cctgtatgac ctggcagtgt ccgcattgtg cggaaacaca
    47941 ctgcttatgg aggacagaca gccgaacgcg gtgagcataa ctacagtcga tcccgaagac
    48001 tctgttttcg cgatcatctc cgatatccat ctgaacaatc cgaaatcatc cggcgacgac
    48061 cccgacgctt ctctaaacac cctgcttctt gaggttattg aggaactcaa ccttatccag
    48121 ccggatttcg tggttcttct tggagacctc gtggaaagca acgccgacac ataccatcgc
    48181 gacttcgacg atatgtggaa tattctcgac ggttacgcca aattccccgt ctttgccgtg
    48241 atgggaaacc atgacgggca gcgcgttgga gaagtggacg gattcgagta ctttcagcgc
    48301 catttcggcc ccctatatca ttcctttgat tggggcgggt ggcactttgc cttggtaaac
    48361 acgtacgacc atccagcaca tccgtcagac aacggggtca taggacgcga gcaactaaaa
    48421 tggctcgata gcgacttgca ctttgcgtcg tctgcagggc agaagatcgc agtcatgctc
    48481 caccataacc cattcgacga taggtggatc ttcatggatg aaggcaggga gcagcttgtg
    48541 gaggttttag aaagacacgg agcaaggtat gtcttcgccg gccacaggca ttcagaccag
    48601 ctcgagcaga cggagctaac aaagataata actactcgca cagcccaggc agcaagccgc
    48661 gacatggcag ggtaccgagt catcagaatc gaggatggct acatcgcatc tgtccactac
    48721 atgatgccaa agtcctcgaa gcctcttgga accacgaata tggggccgga ggcagaaggt
    48781 agagattgaa tatatcggcc tgggcatgga ggacctgatt tgcaagcctc cgtgccctgg
    48841 ccgatagggc catgatgatc tggttcccct cctttcaacc aaggtggctt gcaatcatgc
    48901 agacgcacaa accccatatc atgggaggag tttgggcggc atgtgtggaa aacttgtatg
    48961 gtccacgata atgcgggagg gatacgcttg aaaagtctga tgactggaaa cgaagcgata
    49021 gcccggggag cctgggaggc tggcgtcagg ttcgccaccg cctatcccgg cacccccagt
    49081 acggaaatcc tggagaacat cgcaggctac gacgaaatcc gctcccagtg gtcggtgaac
    49141 gaaaaaactg ctcttgagac agctttaggc ggctccatcg ctggcgcaag aaccatttgc
    49201 gcgatgaagc acgtcggtct gaacgttgcc gccgaccctc tcttctcttc ggcatacatg
    49261 ggagtcaacg cagggctcgt cattgtcacc gcagatgacc caggttgcca cagttcacag
    49321 aatgaacagg acaataggaa ctatgcaaaa gctgccaagc ttccgctcat cgagccatcc
    49381 aacagccaag aggcactgga tttcgtaaag gttgcattcg agttgagcga ggcctacagc
    49441 acaccggtct tgttcagaat aaccacaagg ctagcccatg gcaaatcggt cgtgaccgtt
    49501 ggccgccgtg tggacgtcaa accgcgagaa tacgtccgag acacgtccag atttgttcta
    49561 atgccggcac aggcgagggc ccgccatggt gtccttgaag accgcctgat tcaactggag
    49621 gattgggcat gcaccacccg gctgaatcga gtggagtggg gttctcgaaa actcggcatc
    49681 atcacgagcg gaatagctta ccactacgcg cacgaggtgt tcggcgagga tgcttcctac
    49741 ttgaagctgg gcatgagtta tccattccca aaaaagctag tggagaactt cgccgcacaa
    49801 gttgatcgcc tggtcgtcat cgaagaactc gatccgttca ttgaggacgc cgttcgcgcc
    49861 atgggaatcg aagctgaagg taaagccatc attccccgcc ttggcgaact tgattccgct
    49921 atcatagccg aagcattcga aaaggccgac agtaaaagtc cgccgcctga agatgtcact
    49981 ggaaggcccc cgatgttctg cgccggttgt ccgcatcgcg gcgtcttcta cgagcttgga
    50041 aagcttccag tgacgataac aggtgacatc gggtgctata ctctgggttc gctgcccccg
    50101 tttgcagcca ccgaaacgtg catatgcatg ggcgcaagca tatcggcggg gctaggattc
    50161 cagaaggttt tcaacatgct gaacccggcc gatcccaaga gaacgattgc cgtaatcggc
    50221 gactccacct tctatcactc aggcatgacc ggtcttctcg atgcggtcta taacgacgtc
    50281 ggactcataa ctatcatcct cgataaccgg attaccgcaa tgaccggcca tcaggagaat
    50341 cctggctctg gctataatct ttcgggccag agcgctcccc aagcaaggcc tgaagagatc
    50401 gccaaggcct taggtgtcga acgggtcacc actgtcaacc catatgatct tacagaggtg
    50461 aagaaggcac tcgataacgc gcttgcagac cctcatccat cagtgattgt gtcccgagcg
    50521 ccgtgcgccc ttctgaagcg tgaagcggtc agaaggccgc cttacttcgt cgaccaggat
    50581 gcatgccgaa aatgtaaggc atgcctcaag gtagcctgtc cggcgctgta cgtggaagac
    50641 ggagaggtgc acatcgataa tgcatcctgc gtcgggtgcg gcgtttgtgc ccaagtatgc
    50701 agatttggcg cgatcaatca aacagagaaa acgggtgaga actgatggct aacactacta
    50761 gcattatgtt ggcgggagtc ggcgggcaag gcacgatatt ggcaagccga atccttgcgc
    50821 agctcctcct cgataaaggc tacgatgtta agatgtcgga agttcatggc atggctcagc
    50881 gtggcggaag cgtcaccacg caggtgcgct acggtgagca agtcgacgcg ccgaccatag
    50941 cacccggaca ggccgatttc atactgtctt tcgaggctat ggaagcggca aggacggcgc
    51001 acttcctcaa gcccggagga acaatagttg ccaacacgag aaggattccc tcactcccga
    51061 tactcatcgg caaggaagag tatccggatg atatcgacga gcgccttgca gaacgtggaa
    51121 tcgatgtccg ttgggtagat gcgtattcca tcgccgaaga actggggaat acacgcgtgg
    51181 aaaacgtcgt ccttctcggg gcattttcca cagttctgcc atttactgaa ggcgcgtgga
    51241 aaaaggccat agcccaagtt gtgccgccac gccatgtgga gctcaatgta gttgcttttc
    51301 agagaggaag caggctgacg taaagatgga gacaccgatt gcgctcaccg tggccatttt
    51361 ggggattaca ggggcagctt cgcttatctt cggatatctc ataggccgaa agaacaggtt
    51421 cgacttgatc aacggataca ctgacgagaa gatgctcgat gccgcaaagt tcgctcgatg
    51481 gatgcgacta tgctacaaca tcatgggagg agtgctgatt ggagcatccg ccctgcttgt
    51541 tgccacaaag gcagttcttg caattgtgat tgcatgtacc gtgatagtaa cagtcgtgct
    51601 tgcagtaatg acacttggcg cacgaaggta ctaccgaata tgagtgctcg tgccatacgc
    51661 ccccggctag ggcgcaagct gcggcctggg ggcgttgttc tgtcgctttc ctcgccttgc
    51721 ccgcctcttc gcagatactg cattctatcc cgccaagcta gtgatgatga actaccagga
    51781 ggtttacctc aggcggtcat cggagccatc gcaccccttc ttcatctcgg ccatgcgctg
    51841 cctgattcgc gcttcattgg gattctcccc tggttcgtag tatctgcgcc cctcaagccc
    51901 ctccggcatg tattgctggc ttacccaggc cccaggatag tcatggggat acttgtagcc
    51961 tacgccatgg ccgagggtgg atgcaccgcg atatgaggcg tcccgcagat gaagcggaac
    52021 gtctgatatg gatctggtcc tcacgtcatc cgtggcagca tcaatcgccc ggatgacgga
    52081 gttgctcttg ggagccgtag ctatgtagat cgtggcatgg gccagtatta tccgagcctc
    52141 gggaagacct accttctcca ccgcggcaag agcagcggac gcaaccagca acgcctggga
    52201 atccgccatg cccacatcct cgctggcgtg gatcatcaga cgtcgagcaa taaaccgagg
    52261 gtcttcgcct gattcgatca tcctggcaag gtaatgcaga gcagcgtcgg gatccgaccc
    52321 ccgcatgctc tttatgaagg cagacaccac gtcgtaatgc atatcgcccc cgccatcgta
    52381 tcggagagcc ctatgctgta tggagtcttc agccacctcc aatgttatgt ggatccttcc
    52441 atcctcgccc gctggcgtag agagtgcagc aacctctatc gcattcaatg ccgttcgggc
    52501 atcgccgttg gcaaaactgg cgatgtgatc tagggcatca tcatctatgg atatgttgag
    52561 ataaccgagc ccacgctctg aatcgtccag agcgcgcgca ataaggcagc gcacatcgtc
    52621 gacgcccaaa ggctcaagct tgaatatgcg agagcgcgag accaacggcg ggttgacgtc
    52681 gaagaatgga ttctcagtgg tagcgccgat aagcgtgaca gtgccgtctt caactgacgg
    52741 caagagggcg tcctgttgtg ccttgttgaa cctatggatc tcgtcgatga acagaatcgt
    52801 acgaacaccc tgaagcttcc atctgtcccg agcggttgcc acaacctgcc tgatgtcggc
    52861 aaccccggca gtcaccgcat taagccttac gaagtccgcc ttggtcgtaa gtgcaatcac
    52921 ctcggcgatc gtggtcttgc ccgagcctgg cgggccataa agtattatcg atcctagctt
    52981 gtcggcctct atcgccctcc gcagaagctt ccctggcccg agaatgtggt cttgtccaag
    53041 tatttcatca atggtgcgag gcctcatcct agccgcaagg gggacgcgat cgccccctcc
    53101 tcgcttttcg gccgcatagt caaatagatc agggcctgaa atcatctggc tcacctcggg
    53161 tactgttcaa tgtagcaact tggcatgctc gcccactggc gcaactccat catacatcat
    53221 agaaggcaaa ggtggacaac gaggcaatcg gccggccttc tgcatatatg ttgaggagca
    53281 atagatccga ccccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaact
    53401 atgacgttgt acagaggaga agagtagtct gaagaccaga gcacactctt gcgctttacg
    53461 ccgattctgt tcagcgaaaa acaacgatat ggacggatag caagccaagc gttttcaatc
    53521 gcagaatatg ctgtcagaaa agcccaacag gcttaagctg cccattgggc aaaaaaggac
    53581 cccaccatgc cgtagccgtg gccgtttctc ccccgcgccc gcgaggtggg tgccctccca
    53641 tcacgtttgc gttccacgtt ttcacgcggc ccacacgccg gtgacagagt ccaggctccc
    53701 attgatgatg tgttggaagt aaacagccag agtgcttacg tgcacagcag ggtaatagct
    53761 ccaatcgatg tcaagtacat gatatcacac gacctgcggc gtttcaacaa aaccgatcct
    53821 gccaaaaatt gaggcaattg gatggaatgc gagccgatac ctgcgcgtta gcagcttact
    53881 ccatacccgc tttgccagcc tccaaaacct tcgaggccag ctcggcttgg agaacaagct
    53941 cttgatcgcc tgtggccatg gctttgagcg tcaccatatc cgtcgccagt tcctcgtcgc
    54001 ctatgatcgc cacgaaacgt gcccccactt tcgctgcaaa acgcatctga gccttgagcc
    54061 cgcggtcgag catgtcgtaa tctgcagaga tcccgcaaga acgcatgttg aaggccagct
    54121 cgaatgccct ctcgcgagcg gcctcaccca aggacacaaa atagatgtct atcggccgat
    54181 catacgcttt ttcgccggcc gaggcgcgca tggcaagtac gcatctctca agcccacaag
    54241 caacgcccac cgcaggggtt ggaggtcctc ctacctcttc taccaaccca tcgtaacggc
    54301 cgccgccgcc tatagcgtcc tgggacccga ttcctccaac attgtactca aacgtagttc
    54361 tcgtatagta gtctagtccc ctaacgagcc tcgggtttat cacgtattcc acgttgagct
    54421 tgtccaaaag gcggctaacc tcatcaaaat gactgcggca ttccggacac aggatatcca
    54481 ataccatagg cggctccggg agcgcacggc ggcattcctc cgacttgcag tcaaggagac
    54541 gcaaaggatt cctatcgtat cgcgaaacac aagacggaca cagtttgctc aaaactggcc
    54601 tatacgtctc cctcaaaagc tccctatacg ccggcctgca cgaagggcag cctatggagt
    54661 tgatgttcac aatcactccc cggattccca gagaatcgaa tacctcgagc ggaaccgaaa
    54721 tgacttctgc atctgctgca gggctcggcg ctccaatgat ctccagacca aactggtgaa
    54781 actgacggta tcttcctgcc tgaggccgtt cgtatctgaa catcggccca aggtagtaaa
    54841 gtttcgcagg ttgcggctta ctggcaaacc cacgctctac atatgctcgc acaacaggtg
    54901 ccgtgccctc aggcctgaga gtgattgagc ggtctcccct atcagtgaat gtgtacattt
    54961 ccttctcaac tatgtctgtg gcttcgccca cacctctctc gaagagttct gtgtgttcga
    55021 atatgggagt gcgtatctcc ctgtagccag ccctttcagc cgcaccccgc acgagccctt
    55081 caacgtgttg ccaaagccca acttcgtctg gaagaatatc agtagtgcct ctcggcgctg
    55141 taatatccac gcaaacgccc cccagtgcag attatgaaaa tgggcgggcc caagcccgcc
    55201 ctaagatatc caagatcaca attactcttc aacagataca gactctccgg tgctttcaac
    55261 ctgctctccg gcatcatccg cgtccttcgc ttccttagcc gcctccaatg cctccttaag
    55321 ctgagcctcc caacgggccc tggaagccgc ctcaatctcg gcaatcattt cttcctcccg
    55381 agccgcgtca tcatccatgc ccatctcttt gtaggcatcc cggatcagcc catgcactat
    55441 aggagagaac gtggcgtctt cgcctgccaa gtccgaagcc ttcttgtaca gctcacgtgc
    55501 acgatccttc tgccctgatt ccctcaggtt atctgcccgt gcaatgagca caaaaggttc
    55561 gtcgggccca agcttgagag cgctttcgaa cgccttttcg gaatccttag ccttacctgc
    55621 cgctgctagc gccttaccta gcgatatctg gagatatgga tcctgcggcg tctgcttgat
    55681 ggctttcctg tactgcttca ccgcctcatc gtaatcccct tcgaccatgg cgcggtatgc
    55741 aagcaccctc ggatcatata tagttatctt tgcatctttg cgaagctcat cgacgagctc
    55801 ctgcctattt gccgtctctt tgcggtatct gatcagctgc tcaatgtttt ccttcgattc
    55861 ctcataatcg agattaggct gagttaccgc aatcttgtcg aattcctctt tgatctcttc
    55921 ctcggtcact tcgacaggct ttgctacaga gggataactc ccaataatct gctgtcgacc
    55981 aaggtattcc ttgtagctct tcttggtaaa cccccagttt gccagagctg acacgaattc
    56041 ccgttcgtct gaaaaatagg ccttgtcagc ctcgtaggcc gaatctacca ctacctgatt
    56101 cgccacatac cctgccttct tagctgcatc gagtatgagc gcgtcttcta ttagggcatc
    56161 caaagcatca gaccgcaact cttctatatc ctcaggcttt aggccgccgt attgcacacg
    56221 acgcgagtag gcctgctgga attcgagtgc aagatcgagc gctgacacct ttaccgaatt
    56281 aaccttggct acggtaagcc tggcgctccc ggcgttggca ttggaacccg caaaaagcat
    56341 gggaattacc atcccaagcg ctaaaacacc cgctatgacg gcaacaattg tgccggcttg
    56401 cttacgcatc cctgagatca agataataac cctcccagct aagctcaata gcctacgtca
    56461 ttgtatcgca cgaagattgc agtgtcaacg aatgcgcgac cgtccatgcc acgccttttc
    56521 agctgctata gttgtgtctg ggccatgccc tggatatact cgaatcctct cgtcgaggct
    56581 gaatatgacc tcaaagatag aacgccagag agcctcttca tcaccgcctg caaaatctgt
    56641 ccgtccgact ccgcacgcaa agagagtatc tcccgtgaaa agcgtcaggt cgtcggggag
    56701 caacaggctg attcctcccg gagtatgccc tggagtcgcc aacacttcga actccatctc
    56761 gcccacgcgc aacacatttc cgccctcaag agtatggtcc gctacgagcg aagatccctg
    56821 tcctcccgac cgcgccaaca cgtgaaaaac atcgtctggt tcggctagca tgtccgcatc
    56881 gagagcgcct acgtatacag gagctcctgt cgcatcccgt aacgccccat tgcccccaat
    56941 gtggtcggga tgcccgtgcg tattgattat tgcccggcac tcgagaccat gctgcttcag
    57001 aatattctgg atgcgtgggg aaaaagcccc gggatcgatc actactgcgt cctcatggcc
    57061 ctcacgccaa acaatgtaac agttggttga tatcggccca agcacgagcc tttctacgtt
    57121 gagcacaccc gcatccgcca aataaaaccc gcctccttgc agtctcagtc tgtaaccaaa
    57181 ttcaaaaggc cttgccgctg tcaagcaaaa tcgtcacagg cccgtgatta acaaggtgta
    57241 catccatctc agccctgaat ataccagttt caaccggaac gcccatgttt cttactcctc
    57301 tgacgaattc ctcgtagagg acattcgcat gttcaggcgc agcggcatcc gtgaaactgg
    57361 gccgtcgccc acgtctgcag tctccgtaca atgtaaactg ggatatggca agcatgcctc
    57421 ctccaacatc cagaagtgaa aggttgagct tgccttcatc gtcgtcaaaa atcctcaggt
    57481 tcgggatctt ctctaccatg tattcaacat cttccgagcc atcctctctc gaaatgccca
    57541 gaagaaccac aaggccatgg cctgtgcttc caacaatctc accatctaca ctgaccgaag
    57601 cctcggtcac cctctgaacc acagctctca gcgataagca cctccacgtt gaaccagcca
    57661 cccaatatta tcacaactat cagggctatg gcgacagctc gaacatcaaa cgacgcccgt
    57721 cagttgatcg ccgccctgcg cacactttgg acccctgaaa tccgtctgag cctctccatc
    57781 acagaatcca tgtgctccac gtcatgtatc tccacagtca tgtttatcac agctaccctg
    57841 tttttgaggg tcctggcagt aactgcgctt acatgggcct tcatctccac agcggtgttg
    57901 attacacctg tgagaagacc gggagcgtcg taagcttcca tctcgatctc gacagggtat
    57961 gattcgccgt cctttgtatc ccaatacaca tcgacaacac ggtctttttc agcattaact
    58021 ataatcatgg cattaggaca atctttcctg tgaaccgaaa ccccacgacc gcgggttaca
    58081 tatcccacta tgggatcgcc gggcaccggg ttgcaacacc ttgcgaacct aaccagcaag
    58141 ttgtctaccc caggaacccg aactccatgg gaagacccgg cctttttctt gactggcgag
    58201 ggcttgggcg tctcatctgc agtccgctca ggaacgagtt tggtgacaac ttgggccgcc
    58261 gacagcttgc cataacctac acttgcaagc aggtcatcgg tactctgata actgaacttc
    58321 tgcgccacct cgagcaatcg ctcttccttc atgttcgcac gcacttggag cccgagcttt
    58381 ttcagttcct tctccacaag ctccttaccc tgttccaccg atgcttctcg ttgctcttcc
    58441 ttaagccatt gcctgatctt tcccttggct ttcgatgttt tggctatggc cagccagtct
    58501 ctactgggac ctgcttgcga cttgctggta atgatctcca caatgtcgcc agttgccagc
    58561 ttagtgttga gcggaaccat cctcccgttc actcgcgctc ccgagcatga gtgtcccaca
    58621 tcagtgtgga cggaatatgc aaagtcgatc ggggttgacc cagcaggcag cgtcttcaca
    58681 tcaccgcgcg gggtaaagac gtatacctcg tccgagaaga ggtcgacctt gagcgtctca
    58741 acgaactccg tgccgtttct cgtctctttc tgccactcga ggatctgcct gagccaggtg
    58801 atcttatctt cgaaatcgcc ccctcccccg ccctccttat agcgccagtg ggcagcgatt
    58861 ccgtattcgg cagttctgtg catctcccac gtgcgtatct ggatctccag aggctcgccc
    58921 ctagggccga taacagtcgt atggagggat tgatacatat tcgacttggg catggcaaca
    58981 tagtccttga aacgtccggg aattggcttc cagatgctgt ggactgctcc aagcaccgca
    59041 taacattcac gaagtgtgcc tactattacc cgaagcccca tgagatccca gatttcgctg
    59101 aaatccttgc cttgcttttc catcttccgg tatatgctgt agaagtgttt cggacgtccc
    59161 tggatctcag cggaaatccc gatctctgtg agcgcagcct cgagcgtctc gcgagcctct
    59221 tcaatgtatc cttccctctc ctgcctcttc gtagcgacct tctctaccag atcgtagtag
    59281 gcatcggtat caagatacct gagggatagg tcctccagtt cccatttcat cctccacatt
    59341 ccaagccgat gcgctaaagg agcatatatc tccagcgtct caccggccac ccgtctctgc
    59401 ttgtcaggag gggagaaatc caaagtctgc atgttgtgga gcctgtccgc cagtttgatc
    59461 aggatgaccc gaatgtcctt ggccatggcc acgaacatct tcctcaggct ctcagcctgc
    59521 tgttcctcgc gactcttgaa atccatccgc gacaatttgg ttaccccgtc cacaagcgta
    59581 gcaatctccg aaccaaacac ctggctaatc tcctcgtagc tccttcccgt gtcctctatc
    59641 acgtcatgga gcaaacccgc agctatggtg ttgggatcca aattcatgtc agcgaggatg
    59701 aacgccactg cgaccgggtg gtcaaagtat ggctcgccag actcgcgcac ctgcccgtcg
    59761 tgggcgtcac gagcataatc ccacgcgcgc tctatgacgc taagatcagc atccggacgg
    59821 tttgctccgg ccttggcaag caattcttcc aatgtgcgcg gctcattctg accatggacc
    59881 atccccgccg cccccctttc aaaacctcgg agaggattct ttcttagact acggcactgc
    59941 aagcgcggcc acaaactgcg acgccattat gcatcctggg actttctcag gtctttcacg
    60001 atcaattgcg gctctctgcg accattccac tcattcacct cgagggaata cagaacatcc
    60061 acttctgaaa aaccctgaga cacgtctaca tggctgcctg ccatgcgaaa accgatagca
    60121 tctatcactc gtccgccccg tcccagcttt agcttcaaat gttttccgtc gggccctacg
    60181 ttcctccgct ccagaacatg catgttcctt gtgacaaaga gtggagtcgg attaccgtat
    60241 ccaaagggtt ccagacgttc cagttccatc gcgagctcga tacagatctc gtcttcgcca
    60301 agctctgcat cgacaagcac ccttggaacg agatcctcct ctgtaagcca ttcatgtcct
    60361 atctcgttga gccgttctct gaacaagtcc aaatcttccg cctttattga caatcccgcc
    60421 gcctgtgaat gtcctccgta tttgagcaac agatcagaac attccgtcag agccgaatac
    60481 agattgaatg caggtatact tctggctgat cccctgccca cgtctccctc aaaggatatg
    60541 aggaccgtag gcctgtacgt cagctcagct attctcgacg caacgattcc aataacccca
    60601 ctgtgccatc ggtcagagcc caacacgatt gcgcgagtag tctgtgtatt catcttgctc
    60661 acaggcaccc aggcctcccg caagatggag agctcgatct cttgcctttt gcggttttct
    60721 tcgtcaagca tagcggcgag ctctgcagcc tcctgggccg aatcggtcag gagcaacctg
    60781 gctcccaggc ttggatcgcc aagccttccg gctgcattca ccctaggacc caccgtaaaa
    60841 ctgacgtgcc ctgcccgaat ctcccggcct gaaagatccg atacatccaa aagggcattg
    60901 atgccgtgat ttgaagatcg ccccataagc tccagcccag ccttggctat tatcctgttc
    60961 tccccctgta acggcaccac atctgcaatg gtcccgagag ccacgatatc gagatacttg
    61021 tgtgggtctg ccgcgcacgg cggccttccc gcaagggtcc atacagcctg agcaagtttg
    61081 aacgccacgc cgactccggc cagttccttg aagggatacc gggaatcgtg gcgctttggg
    61141 tctacaacac caacacacga aggcaggata cttccagctt cgtggtggtc ggtgatgatc
    61201 acgtctatgc ccagctcaga ggcataatcg acctcttcaa tagcactgac accgcagtct
    61261 acggtcacca acagcgacac gccgtcgcca gcagcctgct ctattgcttc tctgttaagc
    61321 ccgtatcctt ccacaagtcg attgggaatg tggtagtcca cattcgcccc caaagatctc
    61381 aagacggaaa caagcagcgc aacagaggtt atcccgtcta catcgtaatc cccgtatacc
    61441 cgtattccct caccaatttc cacggcagcc caaagcctag cagccgattg ctccatgtca
    61501 ggcatagcga aaggatcatg catttccgaa gggtcaggca ttaggaaagc cccaacagac
    61561 ttctcatcaa caatgcccct gttcgccaaa acacgggcaa gaacgggcga gatgccgcat
    61621 gttgccgcta gccttttttc tgctgccgag tctccttcac gaagctccca tatcgttctt
    61681 ctaggggcca tagatttctc caccttaatc aggaaccatt cgacttttgt ccaagttccc
    61741 cttctagtca tgtacatgat tcaatactac cacgtttgaa ccctttcgcg ctcgtccttt
    61801 accagctttg ccagcaagtc gcggtcgaac tcatcaacaa tgtacaaggt cccgttggcg
    61861 atctcggtga gcttccgcag aaaccctcga ttgggagcaa gacctatgca ggtgaacggc
    61921 agcttgttcc tggcaatctg ttccgccgcc accagtgcat ctctagcagg gtcccccgtc
    61981 caatagttca tcgtgggtat gccatctgta agcaagacga gcatggtcgg taccccttta
    62041 cgcgacccca aaagcttcac agcctccaca attccagctg cgagcggagt cagccctgca
    62101 ggcctcaccg ttgccaaccc gtcttcaagc gccctgatcg accttgcccg caatacatgc
    62161 tgagttacct tcctgtcctg aaaagtgagg acactcacct tgtcgcggca agtcaagaag
    62221 aggtggcggg cgaggtgttt tgcagcctgt atccgcctcc cggccatact tgcgctggca
    62281 tctatcagca gacacacatc aaccctggcc ggacgtctga cctgctctat acggatatcc
    62341 tccctttgga tcgggctgtg gctcccttcc gttgcgcaac gtacagccca actccgcacc
    62401 gtttcagcaa cggagatatt gccccggtaa tcgacctcag acgcgggaat cacggtccgt
    62461 acaatacccg gtccgtttcc agctctcatg cccttggaga aatcgctccg ctccaggggc
    62521 gtgccacgcg gcctggcgct ccgacggatg atgcgacgga cctggttctc cacctctctg
    62581 gcgaatttct tgagaaacga cgcaagacgc ctgcccttag ctgtaaggac aagcgcagcg
    62641 ccttctccct ccacgatttc caacgttctc agtttcgcca gcattccctc tgcgtcaccg
    62701 aattcccgtt ccaacatggc atagggattc tctcgcctgc ccggaccggc cacgcattcc
    62761 agaaatgatt taaaactgtt cactgagccg aaatcagtgc aaacctccaa agcttgcttc
    62821 atcagatccg gcaggctgtt tcctccatca tacgggattt gctcacgcat gtaagaatcg
    62881 gtcgaacacg aataaggaga aagatcgacc ggcgaagact cggaagagga agcattctct
    62941 atctgctcca atcggcgcag gccaacaccg ctcgaaacga ttgatgcttc aacctcacgg
    63001 acaatgtagt agaccaatcc taccatctca ggcgggagga atgccgcaac gtgcacgtgc
    63061 ccgcggtggg caatcttgag tgactccttc tgtccgaaat tgaggctgcc tgtgacgcgc
    63121 ccgccacctg tcgcggtctg aacatcgatg aagtccgcta gaccagcgat agaccaaatc
    63181 ggtccgccta attccagcag gggcctaggg agtacagaca tctgctgagc ataagcctca
    63241 accgcccgcc ttatcgcttc cgccgccgta cgatggtcca cgtctgccaa ctcgtgaaaa
    63301 atgtctatat gaactacttc atccgtcgaa gaccgctggt agaaaacctg tcccgcatcg
    63361 aggcggcatt ggacctgagc ctgccccgga caatgcgggt ctagcacgtg gacacacctt
    63421 gataccaaag ccgtctcgcc tattctcaca ccacgcccaa gatctagctc accgttgatc
    63481 tcacgggcaa agctaagaag agcatcgatg ttcaagccag atcatcctct gagaacacaa
    63541 gcttcgggcc aatatcggtt atgggccgcg cggggtttgg agggcagacc tcgctcaccg
    63601 aagccgaccc ggctttagca cgggttcccc ctgcataacc ccaacgccct ccttcacctg
    63661 atactccaac atcgtctgcg tcctcctcgc caccctgggc ctgacaatgc ctatcccctc
    63721 cgtctgcgct agcgccttcc tttccgacca ggcgatcttc ggatttcatc ctttccataa
    63781 tccgatggag aatgcctctc aagccaggcc ccccgcaatc tgctccattc ggcttcgatg
    63841 ttgccacctc actgcctaga tttcgatcgc gcccggaagt gttggccgca tcaagcggag
    63901 cacgtgttgt atcccacgtc gccaaggtct cacacgtatc tgcgacagac gccgtcttag
    63961 ataccctcga cttgtcggcc tccgaccgcg caagccctcc gtagcctgcc gtagcattgg
    64021 gacgtccctg cccatggcca gccctttctt gcagtgcatc gacatacctc attatgttcg
    64081 ccagagctga cacgtccact cggtgccgaa gcgctaaggg taggataatg cgaatgtcct
    64141 ccaggcaaac gcagcttcgt ccggccaagg ccgcgcggca ccgggccgcg ctcttcgccg
    64201 actcgagcgc ccttatactc tctatgtcga agtccacgta gagagtggcc aagagctcta
    64261 caagatcatc agggaactct acttgcggcg ccaagagggc cgctgcggtc aaacgccccg
    64321 gccaactgcc gggggcattc atgttgcaga cgcccgcttg acctgtcgcc agctctatcc
    64381 gcccgcccaa gcccgacgga ttaagtcttc ttgacgcatc agtccgctcg tgggttccaa
    64441 ttgcgtcgca ttcctcccat ggcctgcatc ttctcatcgc tccctgtgtc gcgaagtcat
    64501 cgcattccag aatacgacgt atcattgaag cctggctcgt tctgcccatt tccaccgata
    64561 agtcgaacct gtccgcaagc tgccttctta cgtcctcaag gggcccggga tcctcatcag
    64621 gattagatgc agcccagacg ctgatctcca cgggcatcga caccactgga agaccggcct
    64681 cctcgatctg aattctgcct ggtctcgtcc ccatcacatc aagcagcaca tcggtgagtt
    64741 cgggcgaagt gtcggcaagt cggttcacct cgtctacgaa gagaatgccc cgattcgact
    64801 tggggatagt ccccagaagg agagcggcct gaggtttctc ccggtccatc acttgggcga
    64861 gatctatgct cccgacgaca gttcccagtt tggccgaatg ggatatctcg cggaaaggca
    64921 taggtattcg ttccgaagcg agcgcttcca gttcagacaa agggaggtcg cgatggtctg
    64981 ggcagtgagg cgcccatgtc cgacaattct gttcacatgc ggctatccgc tctattgtcg
    65041 ggaggatagt agcggcggat ctgaatatcg tggtcttccc tgtgcctctt acgccctctg
    65101 cgtgcacatg catgggacgt cctgcaagcg aggagacgat ggaaatctcg actgcggcaa
    65161 agagcagggc attgccctca tgtttcacaa gatcgtcgta cgtgcgcaaa acatacccct
    65221 ccgcatcagt gctatacctg aggtagtatt gcaccatgcg tggtctttga ttccagttct
    65281 cctcgaggat gactctagag atatccggag aaatctcgac gaagtgagaa cttatgctcg
    65341 tgtttcgcgc atgacatgtt ccacccaaag gctcactcaa tagctacgtg ctcaaaggtt
    65401 atcggctaca agttagaccg gcaacgtggg ccattttacg ccatcagaag acactgctca
    65461 ttgagaaacg cctggcttgc ggggcagtcg ggggccatcg aagccaaaag ccccgttttg
    65521 tagagtctct cgggcgaaag cctacaaaac ctagcacggc cagacgtgaa agcatttcgc
    65581 gggaacatat cccgcaagga catcactgac gttttccgga gggcaccaca gggactatct
    65641 gatggacgcg tcacttgcca tagaaacaac gactcaccaa taggcctctg gcaactcgca
    65701 agacacacct caacccaatt tgactgaatc gtccagacta caggcaatgc agctacagaa
    65761 tcgcgcagaa tcctgctgat attcgtctac agtaagcaaa ctccgttgat ctccagtttg
    65821 aattcgcaat caagaacctc ggcagcgcgt cggcacatca acatgcgagt aagaaagatc
    65881 tcgaaatagt ccataactgg gcatatctca gtgtcgatag tcaccgtaag cgttatcaga
    65941 cgctgttccg catcgacttc gagaaatgaa cgctccgcag catatgacac cctgtcatgt
    66001 atgtcgaagc ttgcgatgtc gcgcttgctc actcgggttc tgtggacatc gctcttgtcg
    66061 gccagtataa ccgctgctgc gacctcgttg acagggtgac catattcctc ctcgtgattg
    66121 cccaccgcag ccatcacaat cgcgacttca tctggatcca ttcccattcg ggtcaacacg
    66181 ctcatcgcta tgaatgcgct gctctgtcca tgatggtccc gtgagacgga attacctata
    66241 tcgtgaagat aacccgctat tcccgcaagt tccgcagtgc gtgcagggta aaccagcctc
    66301 tccagaacgt tatgagcaat gcttgatacc aaaccggcat gtctgtagcc atgctctgtg
    66361 aatcctatcg ccgccatgtt atgcatggcg caccttatga acgcatctat ctcaggatct
    66421 tgttttacat cttttagagt gattttcttc tccaactcct gtatagcgcc tcccttcctt
    66481 ttcttctaca atccgacttt atcccgatgc cgaagtgtgc tcaaattata aaacggccgc
    66541 gccaagccga cgcggcccgg atatgcattc gcttaaaact agatgtaatc gaatgcgtac
    66601 tggtctatgg gtccgaaggg gataaagaga gtccatgaaa ggctctttat ggttctgccc
    66661 gaaaacttcg gatctacact ccagaacggc attatcgcgt aggtgcgcat cgccacacct
    66721 ttcggaagtg gcttctcagg tacaacagcc gggggctgcg caaagtattt tccccggatt
    66781 gcgccatcgg cttccactac atagaatgcc tccaatgaag ggtagatcaa gcagttgtcg
    66841 gggccggaca cattctccac tatgagatcg aacttgatct caggcggtac ctccagcatt
    66901 ccaacaaccc gaactcgcac tccccctatc tccttggccg tgggaaacag cccttttcga
    66961 taggaaagcg atatccactc tccgccaagc atcttatcgt ccacgaccct gaagcgtccg
    67021 gcaatgtcgc gcagcttgag gtatctgact ctcaactgac cgtcagaatt cgtgtagtag
    67081 accttagcac ggacactatc acctaggttt gcggtttcca cggccgcaat agaaactatg
    67141 ccgtcactct tgccctcgcg agacccaaca tattccaccc acgagagagc tgcgtcgtcc
    67201 gcaagttcct cagcacgccg ccagtcccct tcctggaaag ccatgagata ctcacgtgcc
    67261 acctcttcag ccgtggcagt gctggaagct ccagccgaga agggaatcgc cacaagcaag
    67321 atgagcaaag cgcccaacgc cgtccatctc ttgttagtca ttatgtagag gcctcctcaa
    67381 aatcgccctt cgtcctatga aatctcctct tccgtttcct tatccttggc cacgttatcc
    67441 ttgcccagca catcttggac tccccatttg gccactcgca caatcaaccg ttcgccctgg
    67501 ctggcgacat tgagcagcac tgcatcgtcc ttgatgtcga ctatctctcc gtatattccg
    67561 ccaattgttg ttatcctatt tcccttgcgc aatcccgcaa tcatcgcatc acgctgctgt
    67621 tgctgcttct tctgtggccg gattatcatg aagtagaaga gcaacatcag tacgacgaac
    67681 gggagtaatg ctcctaacag gcttggggcc tgccctgctt gtgcttccat acgctgctta
    67741 cctccatttt cccattcagg ttgtgtccta gttgttatac cagatccgcc attgttcctt
    67801 cctgtattcc gaaaaagtgc ccttaattat cgctgaacga atttcttcca tcatcctcac
    67861 aaggtgcctg aggttgtgta tggatgtgag tctgataccc agcacctcgc cggcttttag
    67921 aaggtggcgt atgtaggctc tggaaaaatt cctgcacgca tagcaatcgc agcctggatc
    67981 gagtggccga aaatcctcag cataggccgc atctctaact attatccggc cttcacgagt
    68041 gaaaaccgtg ccatttctcg caaccctggt cggcaagacg cagtcgaaca tgtccactcc
    68101 gcgggcaacc ccctcgagca atgcgtcggg cgatcccact cccataagat acctgggttt
    68161 gtcctcaggg aggcagggca ccgtctcatc caaaatggcg tacatcacat ctttcggctc
    68221 ccctacagag agcccaccga tcccgtaacc gggaaatccc aaagacgata tgtcagcggc
    68281 agagcgcttt ctcaggtccg gatagacaga tccctgaact atcccgaaga gggcctggtc
    68341 ctctctggaa tgagcctcaa ggcatctttc ggcccagcga gcagtcatcg ccgctgaatc
    68401 cctggcgtac tcgtaatcac acgggtatgg cgtgcattcg tcgaaacaca tagcgatatc
    68461 ggcgcccaaa gccatctcga tctccatgac ccgttccgga gtaaacatgt ggggagagcc
    68521 gtcaatgtgg gaacggaact ccacaccgcg ctcagtaatg gttcgaaggg gcccgagcga
    68581 gaatacctgg tatcctccgc tatccgtgag gatggctcca tcccacgaca tgaacgaatg
    68641 tagtccccct gcctccgcta tcaactcgtg accagggcgc aagtacagat gatacgtgtt
    68701 ggacaagatg atcccggcgc ctatctggga aagctcgtca ggagaaaccg tcttcactga
    68761 ggcctgcgtg cccactggca tgaacacagg cgtgagtata tccccatgcc cagtggagag
    68821 cactcccgct ctcgccatag tctcctcaga tcgcttctca atagtaaatt ccatagccgt
    68881 aataagcatc actccataaa cactagactg catttgtgcc cgctaaccga tgcaagacag
    68941 gcatcacaag atgagcatag catcgccaaa tgaaaaaaag cgatagcgtt cagcgacggc
    69001 ctcgcgatac gcgctgagta tgaactccct gtcggcaaag gctgatacca tcatcaccag
    69061 agtcgactta ggaagatgaa agttcgtaac catggcgtcg actgcgcgaa aggtgtatcc
    69121 gggatatatg aatatgcttg tccacccctc gcccggggcg atggtcccgt caggccgggc
    69181 aacagtctcc aacgttctaa cgctcgttgt tccaaccgcc actactcggc ctcccgaagc
    69241 tcttgcctca ttaatggcat ctgcagcttc cttcgataca tgatagtgct ctgagtgcat
    69301 tacgtgctcc tcgacaacct ctgtctttac aggacggaaa gtaccaagcc ccacatggag
    69361 agtgacacgg acgattacga ctccgcagct tgcgatctcg tccaaaagct ccggcgtaaa
    69421 atgaagccct gctgttggcg ccgcagcaga acccctctcg cgggcgtaca cagtctgata
    69481 cctctcagcg tccttcaggt cagtctttat gtaatggggc acgggcatct cccctatctc
    69541 atcaaccacc ttgtcaacgc cctcgatcgc ctcaaatgac accacgcgtc ccccatattc
    69601 cgtcttatca agaacagtgc cgatcatgcg gcccttgcca aatgacagtt tttcgccgac
    69661 atgagcacgc ttacccggtc tcgccaggca ttcccacaca tctgcctcta tctcctggag
    69721 cagcaagagt tctactttgc cgcctgtgct tctcgtgccc cagagcctgg ccgggattac
    69781 ccttgtatcg ttcaggacca agacatcgcc tgaatgcagg aattctggaa ggtcgcagaa
    69841 gctcctgtgc tcaattgagt tgtcctctct tctcaacacc atgagccggg acgcatccct
    69901 ccgctctatc ggtgtctgtg caatgagttc ttcaggaagc tcatagtcga aatcctctac
    69961 cctcatatgt atatcccttc tccggcccat ctgtggcagc ctcaaatcag gacatttgaa
    70021 caggccaatc cgaccgcagc tgcaagctgt ctacagccgc aacactcggc gtttcccgag
    70081 cttgaaacgc cgttagcaac cttactccga ataccccagc ctgtcaagga tagcctcgtc
    70141 atctcgccat ccacgtctca ctttgaccca tgtcttcaag ttgatctgcg aaccaagcag
    70201 gcgctcagcc tcaagcctgg cacgtgcgcc cgcctcctta agcacgctac cgccggcccc
    70261 aatcacaatg cctttctgag attcacggtc aacgtatacg gtcgcactta catcaacgat
    70321 atcccgatct tccctgggga tcatctcctc aactaccacc gctatggaat gaggcacctc
    70381 ctcgcgggtc aactcgagaa gcttctcgcg aatgagctca gcgatgaaaa actcctccgg
    70441 acggtctaca accatatcgg gaggatagta catgggccct tcaggcattg catcggcgat
    70501 ctcgccaatg aagccctcaa gattctctcc tgtttccgca gagaggatct tgacgcggtc
    70561 aaactcgcca agcgcacggc aggcgcgctc tgaaaccgcc aacgtctcct catctacaag
    70621 gtctttcttg tttatgacga gccatgcagg ggtcccaaga tcccttatgt acgacgcaac
    70681 agtcctgtcg tcatctcggg caggagatgt ggcatcagtc acaaagagta tgagatctac
    70741 ctcctccata gctgttcttg cggcttggac catccttttc ccaaggccgt gatggggttt
    70801 gtgtattcca ggcgtgtcaa tgaagattac ctgcgcgtca gggagattgg ctatagcatt
    70861 tatccggttt cgagttgttt gacgcttatc agaaactatc gagactttgg tcccgatcag
    70921 acgattcaga agagtcgact tgcctacatt aggtttgcct actattgctg taaatcccga
    70981 cttcaactac tccatatcct ccgatgtgaa ggccgctgga agcagctcac gcacggtcat
    71041 agttgaacgc gcttcgcctt ctccggccat gattattgga atatcgagac caaactccgc
    71101 catgaactgg cgacagatcc cgcacgggct gatcggtgta ggcgtgtctc cacacaccgc
    71161 aactccaaga aatcccctct ccccttcaga aatcgccttc acaaaagcgg tccgctctgc
    71221 acatatggtt ccgccgtacg aagcgttctc cacattgcag cccgcatata tccgtccgct
    71281 ttcagcgagg actgcggccc ctactctgaa gcccgaatat ggaacatagg ccgcttcccg
    71341 cgcctcgcgt gcggcccgga caagcatttc ttcagtatct gctgacagaa cgcgcgcctc
    71401 atctgtgcct gtggcactgc ctgacacttt tatccacccc cgagttcacc ttgcccagaa
    71461 ttgatttcaa ccatgttcgc ctccgacacg atctgtaccc acgtattccc agggcacagc
    71521 tctatcggcg tccctgtttc cgcaacataa accgcaggat cagtcgtgcc tgacagacgg
    71581 acccaccggc cgcacatcac ttcccccgca ctaagcgcca ccacctctcc ggaaccaaca
    71641 agttccatag agagcctacc ttcgtcgtcg aggactctag ttggcactac ctcaatgatg
    71701 acgtttgccg cagcaatctg ctgtccggta ttggcatcca catgaggctt cttgttgatt
    71761 gaacgcagcc ataaactcgt ttttgcatcg tattcccatt gcactgagta tccaggcgaa
    71821 taccttatgg tcacttcaat cggattgcta ttaccgcgag cttcccgctt tcggaataca
    71881 tatcctgatt ccgcaaaatc cgttctataa cccattcgcc gagcacaatc cagaattgac
    71941 tcggccttcg catacagatt atgtggaacg cgcctggtgt tgtcgcggta gaacgcactt
    72001 ccctgaacaa gctcgttgac agatgggtaa ttcgactcgg caataatggc aagagcttcc
    72061 tggctgcctc cacaatgggc caataccgtt ccgtggccag acgcgatgtc cacaatgtac
    72121 gggcggacgc ttcttatggg gccaatctcg acagatgagc gacccgagta gaaggcaagg
    72181 aagcgcgtaa tgcctccctc tgccaacatc tcatagacta tcgaggcggc tccaagacca
    72241 cgctggggcc ttgcagccgg cgcattttcg atgaccgcgg caaagtactc tgcatcttgc
    72301 aacggcggca agcctgtcag aggatcgacc cgaataggtt gatctgcttc gtcctggcga
    72361 aaaaacgacc cgggcgatgc gccccttccg atatgcttcg cctcatgcca aaaccttata
    72421 aacgctaccc cggcagtgat gacgagaaaa acgataagca agatttgcca tcggcggggc
    72481 ctcaccactg aaggcctccc ttatgatacg ccgaaaggac caggcttgac tcgcgcttta
    72541 tctcgagccg ctttccggtt ttggcaaatt ccgacgccat cggcctaaga gcccgctcaa
    72601 gcgcgtatgg gtttccaaca gcgtatatac gtattgggtt gactgcaacc ggccgctggt
    72661 ttaccagaac tatcggacct atgcatctta ttgaagaggt agagattatt cgctcgcccc
    72721 caacttgggc accacgggcg cctgccgcaa aaagcctatc cagaatctct gaaacatccc
    72781 tctgatggac gatctcgtca aaaccgtagc ctgaaggagc atcataagcc aagactatca
    72841 cgccttcacc ctccaactca gaaagtccag cggcctctct aagggcgtcc atttcttcat
    72901 ggatggagtc aatctcacgg gcaagcaaat ctgcttgggt tgcgagatcg cccgtatcaa
    72961 aggagattcc ggactggtcg atcacgacct cgatggccgt aggcgacctg gcaatcaaag
    73021 gatccgcgct cagagcttcg cgagtggcgg ctcccaggac gttctgccta tcttggactt
    73081 gcgattcgga tgccccgggg gttatcagga tgtacgcacc cttccctgtg tagccttgaa
    73141 ccccactgtc cgaactgagc aactccagaa cccgccctct cgcgctggaa aggcgatccg
    73201 actgaacggc ctcgtgaaga tcagcagcag cctgtgcaag ctcacgcccg atatcctcaa
    73261 cgttgcgagc agcaatcaaa ttctgggctg cagcggtcag gaccggctct gcactcccaa
    73321 tccccgcaaa ctcgtccctt tcaagcattg tcctttgcat gtccacgtaa cccatggcac
    73381 caagcctggc catgtcgagc gccgtcaact ccccaggcag tcttatgagt ccaacatatc
    73441 gtcctatgac aagagcgtca aacaaaagca caatcgacgc aagcaaaagc agggcaagcc
    73501 aatcgagttc gcccattctg acaactctcg gccttcttac ctttcgccac ttgaccggtt
    73561 gatcattggg cgcggtacgg cgataagcta atgttgtcat accgctccac ttccactctg
    73621 atcccccatc gcttgaggga gttttttatc agatcgaggc tgctctcaag cacctgcgga
    73681 tctcctacag ccttgatcac aactgggttg accggtatcg gcttctggtt tacaaggatc
    73741 tgcggacctg cagaacggat cgacgacgtg gccacgagcc tctgcccgcc gacctcaatc
    73801 ccttgcgctc ctgcagcata cagctcgttc acagcgtcgc gaacatcctt atcctgcact
    73861 acttcgtcac ttgtaaaccc accttcagca tcgtacatcg acacgagaat acccgaacct
    73921 gacagcgccg aatatcccgc agcgcgcctc aactcatcca gagaaagcct gagggagtca
    73981 gtctcgtcgc gcaataggcg caccctatct tgcatcgttc gtggggtaat gacgtttgcc
    74041 ttgccacccg cgatttcgat gtctatcggc gcggctagat cagttaagac tgggctcttc
    74101 tgaattgcgt ctattgccga aagcgatagg acgtgagcgg tatcctcaac tacgatcctt
    74161 ccatcctgtc ctgcagagac ggttatcatc gcctgacccg taaccgcaat cacctgcggg
    74221 tcggaattga ctatgccgag taccgcctcg cgcctacggt tctcctgctc acgctctatg
    74281 acgtcgcccg ttgcccttcc gtagtatccg atgatgtacg cgatggactc cgcgtcattc
    74341 gccttcgata cctcgaactt gaacttggca agagcgtccc tcacagccgt attcctatca
    74401 agaccaagct tcaccgcttg agcctcgtag taatcggcaa gggacatggc cccacggcga
    74461 agcaattccg tattcgagac ctcacccgga aagctaatga gctctgcagc acgggcaagt
    74521 accagaatat tgacgaagag aagaattatt attattcctg taagccattg tctcttcctc
    74581 cgaaccaact tggtcgtttc agtcttcgat ttggaagcca tgtaaacgct cacctcatct
    74641 caaccgtgac aagattgcac taaaaagctt agggccgagt atgatagctc ccactatggc
    74701 cgctccaaca gccgacacca acacggcgcc ggcagcgaca ttctttgcct cagccgcaag
    74761 cggatcatat tcgctggtga ccagatccac gcagtgctca atagcagtat tcaccatctc
    74821 cgcggtaata accattgcaa cagctaaagc gattatgccc gcctcctgga cactcagctt
    74881 aagaaggatt gagactattc caactgctgc agctgcgatc caatgcacgc gaatattccg
    74941 ttcgctcctg aacgcctgtg aaatccctat cccggcatac ctaaagctct cgcgtaaatt
    75001 gcgcgctttc aaagcatccg cccccactta gcgcggcagg cctatccggt tcatcaccga
    75061 ttcctctaac cctctcatgc gcgccgcgtc ctcatcattt tcgtgatcgt agcccgaaag
    75121 atgcagtacg ccgtggactg tgaggtaaca gacttccctg gagagagaat gtccgtagtc
    75181 tttagcctgg ctctcggcgc gttccagcga tatgactata tcgcctaaga gaacgtcttc
    75241 ttctgccgcg aattcgtaat ccatcccctc gtattcagta ggaggaatga cgttgggcca
    75301 atcagggcac tccgttattt cagcacactc gtcgtccgca atcagcgcgt ctggatcctc
    75361 caggggaaag gaaaggacat ccgttggagc ctgtatcccc ctgtaagttt cgttcaatcg
    75421 ggcaatttct gcgtcatcaa caaagctcac atctacttca tagttgtcgt ctcctaagac
    75481 tgtctgtagc gctatcaaaa ccgcctcctc cacctgggcc tctagttcat ctgtgacttc
    75541 gactttgtct tgcctgttca agatctcagc aggcatttcc gtcccctccg catgcttttt
    75601 cggagcactt catctctcga ccctattatc tccctgttcg ggcgaggact cacccgcccc
    75661 gtcctgtgcc gccaccttca cctcttccag ctctcgatcc gtccccggct cgtgcggagg
    75721 caactcaggg tattcgacgc gctcgtggta tactccactt agtacactga taaaagcttg
    75781 ttcaatctcg ctcagctctc tcagagtgag atcgcactca tcaagctgac cgtcggagag
    75841 cttgtccttg ataatctttc tcacggtatt ttggacttgc cacgaagatg gattggacat
    75901 cgaacgcaca gccgcttcca ctgaatccgc aagcatcacg acggctgcct cccgcgtctg
    75961 aggctttggc cccggatacc tgaaatcctt ttcatcaatg caaaggtccc gttcgctctc
    76021 ggcagctcta gcatagaaga acaaaaccag cccggtaccg tggtgttgct gaataatgct
    76081 gatgactggc gcaggcaact tgtactgcct tgccaactcg acgccgtcct tcacatgcga
    76141 cgttatcacc aacgtggaaa gggccggact tatcttgtcg tgcggattgg cctgagcaaa
    76201 ctggttctcc acgaagaaat acgggcgctt caatttcccg caatcatggt aaagagcacc
    76261 cactcgcact agcagcgcat cggcttcaat ggcttgagct gccgtttcag ccaggtttcc
    76321 cacgagaatc gaatggtgat aagttccggg ggcttccaaa agaagccttc tcagaagcgg
    76381 atgaccagga tttgaaagtt caaggagacg aagggaggat gataccccga acaacgtctc
    76441 aaaaaacgga agcgatccca gcgtcatcac ggtcgatatt ataccattca caaatcccaa
    76501 ataccacctg gacactattt ccggctcacc aaatgccagc ccataacaga ctgagactgc
    76561 ggcaaccgct gttcctaccg ccacgaccgc tcgagtcata ttcgccctct catgaaggtc
    76621 atgcgcggag tacgctcctg ccgctccagc tgccagtgaa gccagcatgg caaacatgtt
    76681 gcccctgagt aacactccta caagggctgc cacagacaag ttgacaacaa gggccatctc
    76741 taaagatgcg agaaacgaaa tgatcatggt ggcaaaggca gctggcacca aataggccgc
    76801 gtagtcagga gtcaagttcg caagaatgct ggcaagcagt aaaaccaaga gccccgctat
    76861 ggcaaccagc gacagcattc cctgttcatt gggcgcgtgc gcaggaaaat tctggagata
    76921 aatcgctacg taccccacag cgagcgctgt agtggaaatc actcccaccc agccaagtat
    76981 cgacccacgg gctccgagca ggcccagatc tctcaaaatc tctatcgtct cttgcgttgc
    77041 cggctcgccg cgcctgataa tattctgccc tttctgaaca tagacgggct gaacaagatc
    77101 agcctcttcc tccgcagctt tctccacttt tgcaaggctt agagagaggt tgggccgaat
    77161 cgcagaaacg actatccgcg aaatgggcgc cacgtattca ggcgcaacac ccttttccag
    77221 caacgcctcg ggaatacccg ccgcaatctc ctgcagattg gaatcggtta ttcggttctc
    77281 ccgcattaca ctgccgacaa gcgcaaccgc aatatcccgg gcgtcctcgc gttcagcctc
    77341 atccattgat gccaaaagag caaggtcctc tgcagacacc gaaatgccct cgccatcatc
    77401 gattatggcg gccgcacggc gaccgaccgc cgccaggtct tcttcctcag gcatctcgct
    77461 gcgcgccgaa tccaccgcgg agaagacaga ctctaccgct tcctcggaca tgtaagagca
    77521 tgactgattt atctcgtagt tgtccgggaa actcggagca ttcttcaagt atgtcttccg
    77581 agcctcttcc ctcagcttct cagtagcaac acgattcaca attgtccttt gggcctgaat
    77641 atcacgtttc gcaaactgcc cctcttccag cgtaaccgcc tggggtaaca gcgccgatgc
    77701 gacaatgaga gaccccaaaa tcaccgtcgc cacagcaact gcaatcctac tgctcgcgaa
    77761 ggtcttaacg cttgagccaa atcgcctcgc gctctctttt ctagacagct gtgccaacgg
    77821 catcacccct tctcattccg tctccgccat ctttactctc cccgggacag gtcgacaccg
    77881 agttgctcct tcttagccct ctcagcatcg tatctctcat aggcctgaac tatcttttgt
    77941 accagctcat gtctgacaac gtcctggtcg ttcaggtgga cgaactctat gccatccact
    78001 ccggcaagca ccgtctgaac atcttcgagg ccggaaaact tcctgttggg gaggtctatc
    78061 tgagtaatat caccagtaac cactgccttt gaagagaacc ccagacgagt caggaacatc
    78121 ttcatctgct ccggcgtggt attctgagcc tcgtcaagta tgatgaacgc gtcatccaat
    78181 gtcctcccgc gcatgtacgc aagtggcgcg atctcaatta tgcccttttc cttgtagcgc
    78241 tcaaacattt cgacaccgag tatgtcaaag agggcatcat atagcggccg caagtaaggg
    78301 tccacctttt cctgcaagtc gccggggagg aacccgagct tctcccccgc ctccacagca
    78361 gggcgcgtaa gaataattct gctgacgttc ttggccttca aaaccgaaac agccatggcc
    78421 atcgccagat aagtcttgcc ggttcctgcc gggccaatgc agaagactat gtcattcctc
    78481 ctcattgcgt ccacgtaatg cttctggcct acagttcgcg ggcgaacctg ctttccgcgg
    78541 gcagtaacct gcaccacatc gcgcaataca gaacagagtg tttcaggtct ttcgttctga
    78601 gcaagcatca cagagtacct ggtttcccgg ccggaaatgc gattgcccgc gcgtacagct
    78661 gactgtaagt cggagaggac ccgcattacc ttgctgacag aatcctccgg ccctattaca
    78721 gtcacatcat tggcacgggc tactattttg cattcgagaa gttcctcgac tagccttaga
    78781 ttctcgtccc ggttaccgaa aagtgcagct gcctctgaat tatcattggt gcagaaccta
    78841 gcttcaacaa ccggattgct caaatgagat tactccctcc ttctcacagt tgatgcgaaa
    78901 tagcaatagc cttgctagaa aacatcatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    78961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    79021 nnnnnnnnnt catcgtcata tagttccagc tataacatgc aacgtctcga ccacctactg
    79081 gcgttcccgc ggtctggcta tgtcttctac agtttccaca gtaagaatga cctcgacagc
    79141 ctctggcatt acattctcgt cagataccct ttcgctcaca gatacaatcc tcgcatcttt
    79201 gttgagcttg gctgcgatcg aatcttcagc agcagcacgg gcaagcgcat atgcctcgct
    79261 gcgacttatg cgcgttgagg taagtgcaag ctcgtatcgc tcaattctaa taaattctac
    79321 gccaggacgg tagtcccttc tatactcctt ggtatcgaaa gcttcaaaac tgccaaccct
    79381 gcccagacga aaatcgaggt tgccccataa aacccggtaa tgtattgact tgcgcccggt
    79441 cctcacgcgc tcttccacgt tgagcgggac tactgcgcgg ccctcgcgcc acacgcggcc
    79501 ttcgacgata gccctggcat gtgccgcatc accacccgca cccgacgcgc tttttcctga
    79561 tacaagcacc tggcctgcca cgaccgtctg cccctcagct acacacgcat cgcctgcaag
    79621 gacaatcagt ttcacaacta tggcatcttc tgacgcaatc agatcgattg ggaaaacggg
    79681 agcatccggc agaatctttt cagccacgcg cacgatcagg gcagtacctt gagtctccac
    79741 gcctgcccaa gccaggcctt ctactgattt cgtgatttcg gacctgatgg tgtcgcagtc
    79801 gatagatgac agaagagtcc atctattaac gcccatatca tcgagaatgc ggcgaatctg
    79861 ctgttcctgg tggggattgg cgccaacaac ctctatcgtc cacagaatcg aagacatcac
    79921 atagagaatc gcaacgaaga gcatcagacc gatgaagagg aatttcctcc tgcccaacgc
    79981 tgcaatcaag aacggggctc caacccgcgc atccacggtg gctcggcact ggcaatcccg
    80041 aagaatcgga tgcaggcgcc tgtaatcatc aacgctcacc ctggcggtga tcatccccgg
    80101 acccagagtg gtcacatcat acagccctat gtggcgagcc gccgccatat taacgaattt
    80161 ctcgatcatc ctgcctgtga ccctgattat aacatatcca cgaacccagt cccacaggcc
    80221 ggatgttgcc atctcaggac acctcccacc ctaggaactc aagactgtca aagcgtcctt
    80281 caaccacaat cctctccctg tttattcctc tcaggaccag cttgcgaccg acaattgata
    80341 cttggccgac agtagtctgt atgcataggc gatcagcaga atactcgata aggcccttgt
    80401 ggttttccat cacgagcctg gcattgccct ccaaactgag ccgaggcaaa tcgagggcta
    80461 cgtccttcgg cacgccaagc gcgtcagaca ttcgttcctt tatgcgggcc gaacgcgctt
    80521 cacgcatgtg aatacctccg ctattcacgg agcggctcag gcaagccgtc ccgtccaggt
    80581 tttctgagtg actcgcaaga agtgccttcg tctagcacgg acgatgcaaa cttgagaatt
    80641 gcctagaggc ccccaccggt aaaacggacg tccaggccga tgtcggatcg gcaataaccc
    80701 ccttcaaacc ctatgcgctc caccgcatcg tatgcgcgcc gacgcgactc agtttgggta
    80761 gatccgacag cagtgactcc caacacccta ccgccgtccg agacaagctg gccctcggag
    80821 atcctcgtgc ctgcgtggaa aattaccgtg ccttcaggaa gctcgctagg cactcgaaca
    80881 ggcaccccag tgctataagg acctggatat ccattactgg ccaacactac acaggtggaa
    80941 tagccaggta caaagtctgc cttcatttct ccgatgcgac cgtgacatgc agcttccaca
    81001 aggtcaagca gatcactttc gagaagaggc ataatggctt gggtctcagg atcgccgaac
    81061 ctgcaattga actcgagcac ctttggccct tcatccgtta tcatcagccc ggcaaagagg
    81121 gcgcccacaa atggatcgcc ctcatccgcc atctgcctga cagccggaag cagcacctta
    81181 ttgctgatcc aatcgagcat tgcccggtcg atgccgggag caggcgccac ggcgcccatg
    81241 ccccccgtat tcggtccctc atcaccgtca aaagcccgct tgtgatctct ggcaggcacg
    81301 agcgtcacga catctttgcc gtcagttatg cctataacac tagcctcgta gccctcaagg
    81361 cactcctcta caagcagccg gcagttgctt gctttcgcag attcagccaa agaatccacg
    81421 gctgctcccg cttcctctgg gcctgaggcg actatcacgc ctttgccggc agcaagcgca
    81481 tccagcttca caactaccct tccagcgtcg gatgcgatcg caaccgcagc ctgcctcgcc
    81541 ttggagatat cgtcgaagac tccatagcga gcagtaggta tgccagcccg ctccatcaag
    81601 cccttggcat aggcctttga tgattcaatc ctggcagcgg acgctgtagg gccaaacact
    81661 gcaatcccct gggcagcgag cctatcggca aggccgaggc ttaaaggcag ttcaggccct
    81721 actacgacca aatggcaatc ggcttcctga gcaaatcttg ccagcccctc tacgtcagca
    81781 acatcaatcg ggacacactg ccccagccgt tctatgcctg cattgcctgg cgttgagtat
    81841 acgcgcccta ctctatcgct tgccgccagc ttccatacta ttgcgtgttc ccgcccacca
    81901 gaaccaacaa ccaagatgtt ggggcgggtc gacagacttg acatagacgc accctccgtt
    81961 ccgagtagtc ttcagataat ctccataatc acgtggtcct tctcgaccgt tcctcctaag
    82021 caaacaccag atatccccgg catacggtgc cctacgccga gaaattgcct agtgcttgaa
    82081 atgcctgacc cccgtaaaga ccatcgcgat atcgttcgca tcagcggcct caattgattc
    82141 actgtcccgc aaagatccgc ccggctgtat cactgcgcga acccctgcct cggccgccag
    82201 ccttacagtg tctccgaatg ggaaaaagcc atcggatgcc atgaccgaac cgcgggcctt
    82261 ctcgcctgct tgcctgatcg ctatatctgc ggcccccact ctgttcatct gccctgcgcc
    82321 taccccaacc gttccgtttc cgcgccagag aacgatagca ttggatcgca catgagcaac
    82381 ggtcttccac gcattgacga gctgagcaag ttcttcttcg tttggggccc gcttcgtaac
    82441 gaccgaaaga tcgagcggaa ccgccttcgc gctgtccgaa ttctggacca gaaaaccccc
    82501 gagcacggga cgaatgtccc atcccgcacg cggtttcaag tcctcttccg cgatttcgag
    82561 gaggcgcagg ttcttcttgc catgcagcag ctcaactgcc tcgggcgaaa actccggggc
    82621 gattatgact tcgaggaata tctctgacat cagcttcgcc tcttcgatgc ccacacttcg
    82681 gttgaaagcc acgatcccgc caaacgccga caccggatcg caatcaagag ctgccttgta
    82741 ggcttccaga ggagtctttc ctacccctac cccacacgga ttggcgtgct taacgatgac
    82801 cacagccgtc tcatcatact cgctcacaac acctatggca cctgtggcgt ctgcaaggtt
    82861 gttaaatgac agttctttcc cgccaagctg ttttgcagct gccaagcccc acggcgcgcc
    82921 accggaaaac ttgtaaaacg ctgctgcctg atgcgggttt tctccatatc tcagagcctg
    82981 cgccttggtg cccccgatca cccagtggtc tccgagggat ttcccttcgt cctctgcttt
    83041 ctctgtctgt tccgcaaagt aattggctat ggctgcatcg taggccgcgg tgtggaggaa
    83101 ggcctctgcc gccaaggccg ccctggtggc cctggagact tcgccgtgct cacgcagttc
    83161 cgagattatc tccggatacc gtctaggatt taccacaaca gtcactctct cgaagttctt
    83221 ggcagccgcg cgcgccatcg aaggcccgcc tatgtctatg ttctcgcaag cctcctccat
    83281 ctcagcgccg ccttccaccg tccgctcaaa tggatagaga ttgactacga ctatgtctat
    83341 gggagtcaca tcaagcctgt tcaaggcgtc aagatgctcg cgggtgggct ttgcaagaat
    83401 ccctgcatga attcttggat gaagagtctt gacacgcccg tcaaggacct ccggaaaccc
    83461 cgtgatttct gagacatccc tcacatccac cccggccttg cggagcagcg cggcagtgcc
    83521 cccggtagag attatctcga accccagctc cgccaggctc ctagcaaact cttcaattcc
    83581 ttccttgtcg taaacgctta tcaacgcccg gcccctcgcc accggacatc tctcctttct
    83641 tgcgccctga cgctatttgc atccaaggcg tctgatgaca tcgaccagta ggtcatgttc
    83701 ctgctcgagt atcctcgctg aaagcctctc cacatcgtca ccggccaata ccggcaccac
    83761 tctctgatcg atgatcggtc ctgaatccac cccgtaatcg acgaaatgga ctgtacagcc
    83821 tgtgtagcgc acgccggcct ccaaagcctg acgctgagcc tcaaggcccg gaaatgacgg
    83881 caacaatgac gggtggatgt tgatcaccct gcccaaaaac ctgtctagga acacgggtcc
    83941 gagcactctc atgaagcctg caaggactac caggtcaggt cgggcggcct ctgcacatgc
    84001 cgccaaggcc gcctcaaaat ccgcccgcga cgaatgatct cgcctctcaa tgcagaaccc
    84061 gggaacatgc gccttctcgg cgcgcttcag ggcatatgcg tctggcgagt cggagatcac
    84121 agccgctacc tccgcatcca gccttccccc ctcgatcgca tcgaagatgg cctggaggtt
    84181 ggagcccctc cccgaaacca gcacgaccaa gcgcaattct tacaccccct gaaattccac
    84241 tcgcccgtct ccgggaatta tctcccctat aatccgggca tcctcgccct gatccacaag
    84301 gatgttgact gcttcccgcg ctttttcggg acgcactacc actagaaatc cgatccccat
    84361 attgaaaacc ctaaacatct cgtccgccgc gacccctgcc gtccggctta tgacatcgaa
    84421 tatcggaggc cgacgccacg tgtcgactga cagcgccatt gaggctccgt gaggcagaat
    84481 cctaggtgga ttctgaacaa gcccgcctcc ggttatatgg gacatgccaa gcacctccac
    84541 acgctcgatc aaagcaagta ctggcctgac atatatcctg gtgggctcga gcatctcatc
    84601 gcctagcgga cggtccatac cttccgggat atcggaaaga aggaaccctc ctacttcgca
    84661 taaggccttg cgcgcgagag aatacccgtt ggagtggagt cccgacgaag ccaaaccgat
    84721 cactctgtcc ccaggacgaa tcttcgaccc gtcgattatc cgtccgcggt ccacagcgcc
    84781 aacggcaaac ccggctatgt cgtattctcc atccgggtag aatcccggca tctcggccgt
    84841 ctcgccgcct atgagggcgc atcccgcctg cctgcagcct tcagcaaccc ccccgacaat
    84901 ttcggctatc tgccccgcat ccagccttcc acagccgatg tagtcgagga agaacagggg
    84961 gctagccccc gacaccagaa catcgttgac acacatcgcc accgcatcga tgccaatggt
    85021 gtcatggcgg tcagctgcga aggcgatctt tagcttcgtt cccaccccgt cggtgccaga
    85081 aaccagaaca ggctccttca tacctgcaaa atcaggtttg aagaggcctc caaaacctcc
    85141 gatatccccg atgaccccag gggtgaacgt gctcttcact gcgggcttca tgagctgcac
    85201 cgcccgctct ccctcatcga tatcgacacc tgaatcagca tacgacaagc tctttggggg
    85261 cctgcatttc ccatgacagg agatggcgtc gggatatcgc ccatccaggc acgcggtaca
    85321 gaattccaac ggctcaccag tatcgcgcgc cgacgccagg ccaccaatgg cttccagcat
    85381 tccatcgatg gacagataat gcagcgcatc ggcgcctatc atctcgcgga tctcgtcttc
    85441 actcttaaca gcagccacaa gatcccgcct gctcgccgtg tcaatcccat acgtgcacgg
    85501 gaacttgacg ggcggtgacg agaccgccat gtctacccgc gtggcgccgg ctttccgcat
    85561 caagtcgaca aggcgggctc cggtagtgcc ccgcactatc gagtcatcca ccaacaccac
    85621 gcgcttccca gcgcacgatc tttccaccgc gttcagcttg agcctcacgc ccaactcccg
    85681 ttccatctgg gagttcctga tgaaggttcg ccccatatag cggttcttcg taagtccttg
    85741 gcggtacggg attccgctct catccgaata tcccagtgcc gcagtgacgc ctgactctgg
    85801 aactgcaacg acgacatcgg catcgaaccg ggcttcccgg gccaacatgg cgccaaaggc
    85861 ctcgcgggct gcggcaacgc caataccttc aatgatggaa tcaggtcggg cgaagtagat
    85921 gtactcaaat atgcaggtgg cagagggaca gctcctccta gaggctcgag tggcgatacg
    85981 tttcattccg tcatgggaga tgactacgat ctcccctctg ccgacatcgc gcttctcggt
    86041 tatcccaacg gcatcgagag cacacgactc ggaagacaca gcccatcctg cagggcctga
    86101 ccctgcaatc aacggcctga acccataggg atcgcggacc gcatatatgg tgtcatcgac
    86161 catcaccacg aaggagtagg ccccttccaa ttcctccata gcacgttcga tcgcgcccac
    86221 catgcccagg gaaaagtagc gggctatcaa ggaacctatc acttccgaat cagattcgct
    86281 ttgaaagacc acacccaacc ttcccagcct ctcccgcaat acacgcccgt tggtgaggct
    86341 tccgttatgg gcaacagcca tcatcccgcc tccgtgacga aagacaaagg gctgcgcgcc
    86401 tgagacgtcg ttgtcctggc acacgccatg cctcacatgg cctatgcccg tctttcctgg
    86461 aagcctcttg aacgcgtcgc cttcgaaaac ctctgtggca aggccagctc ctttcacaac
    86521 ccggatacgg gatccatcgt acacggcaat ccctgcactc tcctgccccc tgtgctgaag
    86581 ggaaacgagt ccatagtaga ctgcaaccgc ggcatcttca cgaaaacagg caccaaatac
    86641 gccgcattct tccttcagtt tgtccgcttc ctgcgctctc agcgctccat ggcgcacggt
    86701 atcgcctcct cgtaaacctg ggccaatttg gcgacgggca gagcgatgac aacttccgcc
    86761 tcctgggccc gccggtcatc gccttgcaca ggaacacaag cagattcaac gcacgtgata
    86821 tccagagact caccgcccac ctcgcctatg gctttcgcgg caacgcccat ctcgtccgcc
    86881 aggtcgagca gggcccccag atcgctctca tccacactga taatggctct tccagcgctt
    86941 tcactgaata ggaacctatc gggagcaatg ttcgcctcca gcctcaccct agcgccacaa
    87001 gaccctgcga tgcagcattc ggccagggca acccctaggc caccatctga aagatcatgg
    87061 gcagacctgg taatccccct gtccgccgcc gccagaagga gctcacacag acgcctctca
    87121 acgtcaagat caagttcagg cacttgacca gcctctactc cgtggaccgt tgcaagatac
    87181 tccgacgctc ctatctcgtt gcgcgtcgtt cccaggagaa caatcacgtc cccttcttgg
    87241 ctgaatccca ttgctacgca atcctcgaca tcatcaatga cgcctaccat gccaacaaca
    87301 ggagtgggac gtatcgccat ttcgccgtct tcgttataga aactcacgtt gcccccggta
    87361 atcggcgtat tcagagcgat gcatgcctcc gccatccctt caatgccctc tttgaactgc
    87421 cagaaaactt cgggcttata cgggttgccg tagttctggc agttagttgc agccagtgga
    87481 cgggccccgg tacaggccaa gttccgagca gcctcggcaa ctgcgtgggc agcgccgaga
    87541 cgaggattga cataacagta tctggagttg cagtcgcagg tgagagctat gcctcgggac
    87601 gtgccgggaa gacgcactac tgcggcatcc gatccgggcc cgactactgt accagcgcca
    87661 acctgctggt catactgcct gtaaacccat ttcttgctcg ccaaatttgg tgagcccaga
    87721 agctgaagca gagctccgcc cagatccttc ggggcctcca aagcgccgac atcatacaat
    87781 gccacttcat cgaggtactt ggggcgcacg gactccctat ggtacagcgg tgcgttttca
    87841 gcgagcgcct tggcgggaac ctgcgctact atttctccgc cttccaagag agttatgaat
    87901 ccatcatccg ttaccttgcc tatctcctca gccagcaggt cccacttctc cagcacgttc
    87961 ttgacttcgt cttctttgcc cggcacaggc actaagagca tgcgttcctg agactcggaa
    88021 agcatcactt cgtatggagt catcccatct tcgcggcgcg ggactttatc gatatcgatg
    88081 accataccca cattgcctcg cgcggccatt tcacacgagg aacatgtaag tcctgccgcc
    88141 cccatgtctt ggattcccag gacacagccg cgggcaacca actccaggca tgcctcgaga
    88201 aggagcttct ccctgaacgg gtcgcctact tggaccgccg acttcttttc cacggcctct
    88261 tcagttaggt cggcagatgc gaaggtagct ccgtgaatcc catctcggcc cgtggtagat
    88321 ccgattagca tcaccgagtt gcccacaccg ctggccgcgc tgcgctttat ctcatcctgt
    88381 ctcatcacgc cgacgcacat ggcgttgaca aggggattat tctcgtagga ctgatggaat
    88441 actgtctgcc cgcccactgt gggaacccct acggcatttc catacccgga tatgcccgaa
    88501 acgatgccat cgaaaagcat gcgagactta ttagactgca gatcgccgag ccacagcgag
    88561 ttgagggagg ctatcggcct agcccccatg gtgaatatgt cccttattat accccccacc
    88621 ccggtagcag ccccttcgta gggttccacc gcggacggat gattgtggct ttcgatctta
    88681 aaggctacag cgagtccatc acctacatcg actatacccg cattttcccc tggaccctga
    88741 aggacttggg ggcctgaagt aggaaggccc ttcaatacgg ccctggaatt cttgtaggag
    88801 cagtgttcag accacattac ggagaacatt cccaactcca cataattagg ttctcttccc
    88861 aagaggtcct gaatcatctg atactcctca tccgacaaac ccatcgatcg ccaaatagac
    88921 tctttaaggc tatccgtctt ggccaaccct tacaccccca cgcacccatt ctgcgactga
    88981 ttcgaatatc cgcctgccat cagtcccgcc caagatcgcc tcgcatgccc tctcaggatg
    89041 aggcatcatt cccactacgt ttccagcctc gttgctcact ccggctatgt tgttgaggct
    89101 tccgttagga ttagcctcag gagtggcatc gcccagctca ttgcagtatc tgaagacgac
    89161 ctgtccacgt tcttcaagct cggcaagggt ttgaggatcg gcgtagtaat tgccgtcggc
    89221 atgggctatg ggcagtgtca gcacgtcgcc ctctctacag cccgatgtga atggagtcct
    89281 agtcgactcg atgcggaccc ttacacgatc acagatgaac ctagttccca catttctctg
    89341 catggcacca ggcaacaatc ctgcctcgag gagaatctga aaaccattgc atattccgat
    89401 caccggcctc ccgtcgcgtg catattccac caccgaatcc atgaccggcg agaaacgcgc
    89461 gattgcgccc ggtcgcagat aatctccgta tgagtaaccc ccgggaagca ccaggcaatc
    89521 gtatcctgcg accgacgtgt ctcgatgcca gatgaattcg gcatcaaagc ccgcagcctc
    89581 gattgcgttt cggcaatccc ggtcgcagtt ggagcctggg aacactatga ctccgaactt
    89641 catttcccag actcctcctt gatctcatat tcgtacgtct ccatcgtagg gttcgccagg
    89701 atctgcgaac agatctcgtc cataatccgc ctgcattcgg cctcatttgc agcctcaagg
    89761 tcaacgcgga ttctctttcc aattcgcact ttctgcactc caccataacc catttgaact
    89821 agagcagacc ttaccgcctc tccctgcggg tcgagtatag actgtttgag ctgaacatca
    89881 acgtaagcca cgtacttcac tccatgcacc tccaaaacga cttttgtttg cgcatcatct
    89941 tcatgcttcg gtctaagtga tttggccatc tgagccctgc actatccctg ccgcgcacag
    90001 agaattcact ccgccccgtc ctgtggggct tgggctttgg catcagccag tctgaccttg
    90061 tgtgccaaag cttccctgtg ctccagcaag cgcttgcgaa gctctccgtc cgaaacggcg
    90121 agaatctctg ccgcgagaag cgcggcgttg gcggctccgt caattgccac agtcgcaacg
    90181 ggcacacctg acggcatcat caccgtcgaa cagagcgcat caaagcctcc cagggcaccc
    90241 ccggacagcg gcactccgat tactggcagc acagtatgag aggcaatcac tcccgccagg
    90301 tgtgcagcca ttccggctcc agcaatcagc acagagagtc ctcgttcctc ggcactcttg
    90361 gcatattctg ccgcgtcatc aggcgtccta tgagccgata tcactctcat ctcccactct
    90421 atgtcgagag cggaaaggac ttcggttgcc ttcaacattc tgcccatgtc agaatcgctg
    90481 cccatgatca caccgactcg gggccgtgca caagtcgccc ccggctcctt ttcactacgt
    90541 gaaatggctt gtccaagtga tctgcgggac acctcactac ccatcattgc catcccttac
    90601 cacaacctcc accttcattg tcgtattctc aaaaagttta gccccggcgt taggttatga
    90661 taacgaaacc taaccgccgg ggcttttatc cctcaaggtg tagcgccact caggacgcct
    90721 gcgccgctcg gaccaagccc tccgcaaaaa agagcagagg cggaacccta ggcgcacttt
    90781 cgcaaaagag cgtattcaat tcatctctac actaatacct taacccacgc cgcggggcgt
    90841 gtcaactcaa tacctgcccg cacgtcgcag gtgcatgcaa aaatcgaagg cttcagcatc
    90901 cccttaactg cgagggagca tcatgggatg cgcgtcgttc cggcccgctt tctgcatctg
    90961 gggggcccaa ggaccgtagc ccagatgatc ccggaaacga cctgttttcc gaaagcctct
    91021 ccatctgatg taagctgggg tgatacgaca tccgcttccc actcctggac aagttcgcgt
    91081 tcgaacaccc tacttgcttc caggctcgtt tcgatttcca gatcgaggat tacatcatct
    91141 acatcggtgg cccgcggttc tatcgtcctc tccgaccact ccgaagcatc gtcccccgcc
    91201 tctcccagag aacccgtcac gatgggctca gccatctcca cttctcgtcc ataagcccgt
    91261 tcttcggcag cctcctgcgc caatgaacgt ctcgttttcg gcagcggcca cgcatcgtaa
    91321 tcttcttccg cttccacaga ttccagttca tacgactgca gagtcgatgg gacttcagga
    91381 gatcccgcaa atccatcagc aggcgccgac ggctccatgc ggccctcagc ctgagcatcc
    91441 ctggaagctt caagatgtcg agccttcttg agagcttcct cctccgcctg cttgaaccct
    91501 ctagcgacaa tccgcagcac gtaagagaag ataaccacta tccatacaac cagcgctgcc
    91561 aggtcgctca tatccatacg gccaacacct ccatccccgc tccgcggcgc acagccaaac
    91621 aaaagatgct ttcacgctgc cgtatcgccc ggaccttgcc gtacgcatca ggcagcacta
    91681 cttcaagcca gcatccgaac cagagccctc agaagaacct ggagcaccag tcgctttcga
    91741 gattgaatcc cgcatctggg tgtcggcctt gagattgagc aggttgtagt agtccatcac
    91801 cccaagatta cctgagcgca atgcgtcggc aagtgcccgc ggcacctccg cctctgcctc
    91861 cacaaccatc gccctcatct cctgaacctt ggccttgttc tcctgctcga gggcgagagc
    91921 tgcaaacctg cgctccgcgg ccttggcttg agcgatgttc ttgtcggcct ccgcctggtc
    91981 catctggagc cgagccccga tattccttcc cacatccaca tcggcaatgt cgatcgaaag
    92041 aatctcaaaa gccgttccgt catccaaacc cttgttcagc accgttctgg agatctggtc
    92101 tggattctcc agcacttcct tgtgactctc tgcagagccc acagtcgtga ctatcccctc
    92161 gccaaccctg gcgatgatag tcgcctcgcc ggcgccgccc acgagtctgt cgatgttcgc
    92221 tcgcaccgtc acccgagcct tggccttgag ctcaattcca tcctttgcaa ctgcggcaat
    92281 caccggggtc tcgatcacct tcgggttcac actcatctgc accgcttcaa gcacattgcg
    92341 gcctgcaagg tctatggccg ccgccctttc gaaggtcagg tttatatcgg ccctttgcgc
    92401 cgcgatcaga gcatcgacaa cacgatccac gtttccgccg gcaaggtaat gcgcttcaag
    92461 cttgtcgacg gtcaccggaa tgccaccctt tgctgccttg accattggca acacgatcct
    92521 gaccggcgga acgcgacgca gtctcattcc gacgagagtg agaagcccca cgtttactcc
    92581 tgctgcccat gccgaaatcc aaagccccac gggaatgaag ttgagcagca ccagcagaag
    92641 aacgaggaca acaatcaaaa ttatccagcc tatagccatg caatcaatcc tcctgatcca
    92701 tttgggtaat aggcctcacg aataccttgt tgccttctat gcgaatcacc cggacgcggg
    92761 ctcctcccat aacgaagggg ccttcggata tggcctcaac cctgcgaccg tcgaaatcca
    92821 ctatacctac aggtttgagc ggggtaacaa ctatgccctc agcgccgtaa agcgattcgt
    92881 acgtctccac ggctaagtat ccttcctccg gcgtttcagt gtgatcaagg accaatttcg
    92941 ccggcgcacg accctttctt cctccacggg agagcaagaa tatcaccaac gccacaacgg
    93001 ctatggctat gcctatcgct atccctagag ctgcagcgcc ttcgccaggg gatgggtagc
    93061 taagtacaaa gctggcaatc acgcttacaa tgccgagaat gcctgccacg ccaaaccctg
    93121 gtattacaaa cacctcaagg agaagcaagg tcaggccgac cagaaacaga acaatgactt
    93181 cccatccggc aaggcccgcc attatcctgc ccccgaagaa aagaccgatg gagacgattc
    93241 ctatggttcc cggaatccca aaccccggcg tcaccagctc tacagccaga cctacaaggc
    93301 ccagcgtgag aaggacctct gcagcaacct gattcgtgag aagcctggcg aaccattccg
    93361 ctgaagtctg cttcacatcg acgatctcag cgccagacag gcccaatagt ccgagaagat
    93421 catctaggtc cttcactttg tattcgataa agccaagatt ctccgcagca gtggctgtga
    93481 gggtaagtat cttaccgcgc tcgatcacgc cgggaatctc gacgtccgca tctaccatcg
    93541 ccccggctac agccggatcc ctgccccgaa gctccgctgt tgcctcaaat tccgcccgca
    93601 ccgcagagat gtacttttcc tctttgggtc tggtctcagc agcgcccatc gaactcccca
    93661 ttttcatggc aatatcatca caggcaatcg cgatgagagc gcctgcagac cacgcgcgcg
    93721 aatcgacata cgctatagtc ctaacttcag aagagatgat ggcatcgcgc atcttaagcg
    93781 cggaatcaac ataaccgcca ggcgtgtcaa tgtcgattat cacgacatcc gctcccgcat
    93841 cggaagcttc tcgtagccct cgcgctatga aggacgccaa tccgtgctcg atctgcccct
    93901 gaagtggaac gatatatact ttaggagctg cggcgcactt gccagcagga accagtaacc
    93961 ccacgacaat cagcacaaga acgaaaaccc ctattacgcc tcttttcgct ctcaagcagg
    94021 agtccctccc ctctcttatg tcaaatggaa taatgccgat cccaagcagc gtcaaaagcc
    94081 gggcaacagc gcagtcgccc tcgcggctca ggcgcgggtc tgcatgaagc tattcctcgc
    94141 cgccttgggc gtcaagcatc tctctgacca tactgtttac cagcttcccg tcagcacgcc
    94201 ctctcagttt aggcatgact gcgcccatga cttttccgat atcccgcggt tcctctgcgc
    94261 cggtctgagc gatcgtttcc gagatgatct gcctcagctc atcctctgat agttgtcgcg
    94321 gaaggtacga catgattatt tccatttctt ttcgttccac gtctgcagca tcgaacctgc
    94381 ccgccttttc atactcttct atcgactcgc gcctcatctt cgcctgcctc gccattacat
    94441 cgacttcttc agactcagac aacggcgcgc ctttctcaag ctctgcattc ttcaacccgg
    94501 ccaaggccaa ccgtatcacg gattttttca gttcatcttt ggatttcatc gcgatcttaa
    94561 gatcgtccct caatcgcact gcaatcgaca ttccaatcac catcccaaaa tgcatcgaca
    94621 tcttgattat accattcgcc agttctcctt gaaacagaag aagcctcgcc gatctcgatc
    94681 aagccattac gcttgcggtc ggggtgaagt catcgttgtg ccttgccttt ccactttcag
    94741 gcttacctcg ggaattatct tgatttcctt gaatatgtcg cgccagatcg gcgcgatttc
    94801 ttcttcccac gcatcgctgg aaatgcgcct cctcactgct tccccaattc caaaaatgtc
    94861 cgtcccaagc cgattctgcg caatctctat caatgattcc atttccgcct caatctggtc
    94921 cgaaacgcca cgactcaagt tccgatccgc ctctatcgaa acaaactgct cctcggacat
    94981 cacttcttct atgtatgcac aacctgttac ccgtatccgg acttccgccc cccgcaagcc
    95041 cgtcctggtc atcttatgtc tcggcggaag ctctatcgtg gcccgagaat gcaatacctt
    95101 cacgccgaca gtgtctcctt ctgttcctgg caccatatgt agaaatgaat attccttcag
    95161 tctgtttcta gccatgagaa ccatggcagt ctgcacggga tcaagccaac ccacgaccaa
    95221 gtccccatgg agggcagcgc tcccgcttat ctcgatgtcg cttttggttt caacatctcc
    95281 ctttttaccc tttccgcctt catttccagt ccgctgtccc caaacaccct gctgcgcctc
    95341 gtcctgctca ccctcttcct cctcaggttc acttttcacc agactgactc tggcaagaac
    95401 aggctgatct cccggttccg cagccgtgct caacaaatct ctgaacctcg attgcggagc
    95461 gaccgacgaa agagttgcat gacgcatcat caggttgatg taagatgaag ccatgttctc
    95521 ctggtctgcc tcgacttcga atatcttgcc gacagggtca gatgtcacca atacccacga
    95581 gttatagcga aactgggagt ccctggccag ataatccagg ataggcgtca agcccttcct
    95641 tgcgatcttc tccccaataa ccagcacctt tacatgaccg cgaaacagcg tctttgccga
    95701 tttcattctc aacgcagaca tggcgtccat cattgtgttt ccggcagcag agccaagcca
    95761 agcaggggta ttggccgctc cctccggacc cgaggacgga agagccatct ggacccagac
    95821 aacgattttg ccgtcatccg actcgtccac tcccattccc atgatgaacc caatatggtc
    95881 gatatcgcgc gcatcccaac aaccgccaag agcgaaaacc agaatgacga cgcagagcac
    95941 gcatgctgcg actgtgagac gtctgcccaa tggatgcgag ccgtggtcgc tcgaaacatc
    96001 gacataatcc atgttatctg ctgtcatctg gtgtaccacc cttctcgtgc cgcaacatgc
    96061 gagcgccgat cagcagcaag gcgggaagcg caatagcata ccagacagtg tacttcaaga
    96121 aatgctccac tgctgcaaat gactgagcta catttgcagg gatagagctc agcaccatcg
    96181 atataattcc gaatggaagg atcaatcgcc tgtagttgtc caggttgagc aggtctgcaa
    96241 tggccgttat ggctgcatag aatactgaag ccagcttcag gatggcaacg gaaaaccaga
    96301 gcgccaggaa gagaatctct cctctttcca gaaactcccc tgctccaacc attcgagcgg
    96361 tctctaccgc ggggtaagtg acgcgtgata tcccccctgt gcccagcatc ataatgccgt
    96421 atgccactgt cattgccatt atggccgtcc ctattgccac acccgttccc actgccctgg
    96481 atacatgacg tttgtccttc aaatgcctga gaaatgcgag cgatacgatg gcctcgcctg
    96541 tataggaggc cgtaagcaag gctgccctca tgatcggaat cacgcccctg cccgccaacg
    96601 gcaagagacg ccccggaacc aacacaggaa tagcgaaaac ccccaaaatg gcgatggata
    96661 tgataaccac tccgaagaag atgaaacaaa cgcgggctat cgcctcgatg ccgaggtagg
    96721 cggcgtagct cgccaggata aagaagacag cggtagttac gtagaatggc gttctttcca
    96781 gaaacgagat agatatagcg agcacgaatt cccgtgtacc aagcacgacc acgacagtgc
    96841 aatagagtgc gaaggtgagg ccgattacct tgccgaatgg tttgccgaat atgtcttctg
    96901 cgatctttac cagattctgt tcaggaaagg ccgatgagac cgatcccacc agccatgcaa
    96961 ttaccaaccc tattacgccg ccgagcagga ccgatatcca tgctgccgtg cctgctacag
    97021 gcgtcaacaa cctggcggct gcaagaaacc ccgtggaaaa cacaatgacc gcaacgagca
    97081 caaccacctg cgtgtagtaa atctcatcct ttgcacttcc gcctattgtc catgcacctc
    97141 tcgtccgtta tgtctaggtt tcatcattgc catagcgact gccaccagcg cggcactccc
    97201 gaccggaggt gccacccttg ctgacgcgat cgccttatca gccccgcccc ttcgtattct
    97261 cctcctcccc agactgatct cgggaatccg caagcgcctc cccatcctgt cgcgccttct
    97321 cggccacctc tggaatccat gtccgcatga aagctttctg gcgtatctga tcctgtggcg
    97381 acggatactc aggacgcctt tgaaacgtgt actcgggcga acgccatatg acgtcgcgta
    97441 ccaactcctt cggcttcatc attggctcca gatacggaac gccgaaagat gtcaggcccc
    97501 caaggtgcaa ggcaatcgcg attagcccgg cgatcagccc aaaaagcccg agactggccg
    97561 aaagaaaaag gagcggaaag cgcagaagcc tgatggccaa acctgtagga tagtgcggga
    97621 ttatcaacga cgacaggccc gtgacggcaa ctacgatcac cattatgggg ctgacaaggc
    97681 cagccgaaac cgcggcgtcg ccaagcaaca aggcccctac aaaaccgaga gactgggaga
    97741 tcgggtcagg catgcgtata gcggcctctc ttatgagctc gagcgccact tccatgagaa
    97801 gcgcctctac cagtgaagga aaaggcaccg gaacgtgcga accggcgatc gcaagggcaa
    97861 gtttggtggg aatcatttcc tgatgaaagg tggccaccgc aacatagaga gcaggaaaga
    97921 tcagagctat tacgcctgca atataccgca acgtcctcag gggtccaccg aaggggaacc
    97981 tctcatagta gtcttcggct gactgaaaaa agctggcaag attcgcggga atcatgagag
    98041 ccataggcga tccgtcaact agaattccga cgagcccttc tgagagggct gcgacaaaac
    98101 ggtcaggcct ctcagtatac tgcacctggg gaaatggaga ataggtgtgg tcctctatga
    98161 gttcctccaa aacgcccgaa tcagtcatga tgtcaacgca tattgcatca atccgttcct
    98221 tcactgtatc caccaggtca gggtctgtga ggccttcaac gtaggcaact acgacatcag
    98281 tcttggatct cactcccact atagttgcat ccaatctaag gtcaggtgtt ctcagtcttc
    98341 ttcttatcaa ggcagtgttg gccctgagca gctctgcgaa accctctctc gggccgcgca
    98401 caaccttctc agtagcgggt tcctctatcc cccgatgctc ccaacccttg gcttcaaaga
    98461 gcacgcattc aggacttccc tcaacaaaca aggcaccctc gccagtcagc acgtgctcca
    98521 tcgccaattc catgcttcga acgacggtga tctggcccga cggcaccaag tgcgttgaga
    98581 tgacagtggc aatgttctcc cggtcgagcg ccccggcatc caaggctgtg gtcctttctg
    98641 ccagcatcac cgatcttagg gccatcatgt tgatcacatc gcgcgaagtc agaccctcta
    98701 tatagaaaag caaggcatcg agttcaggag atgtccatat caccaatcgg cgcaggatta
    98761 cgtcaggact ctcgttgcct aatctttcac ggaggaactt ctcgatcccg tccaggctgt
    98821 cggggatcgg ggtttcgttg gcctcatgtt ggtctgggcc ttcaccaccg ccagatttct
    98881 tctcatcaag cgataccggc gtcttgccaa gaaccatgcg aggacgcctg gcctcccctt
    98941 tcagcagcct gcgtagacgt gtcaaattgc gcttcccccg aa
//
LOCUS       DAOSYL010000004        85823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_207_length_85823_cov_7.782763, whole genome shotgun sequence.
ACCESSION   DAOSYL010000004 DAOSYL010000000
VERSION     DAOSYL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 85823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207_length_85823_cov_7.782763"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1917
                     /locus_tag="PLU15_01730"
     CDS             361..1917
                     /locus_tag="PLU15_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO29674.1"
                     /translation="MATAIKFAEDHADLTEEIASALWGYAEIGLAEYKSYVKARSVLA
                     EAGFEIIDSAADIPTCLVAQWGSGNPVIGIYEDIDALPGVGHGCGHNLNTAAGIVAAM
                     AMKHAMETHDIQGTIKVFINPAEEVWDVAPLVAAAGYYDDVDALISFHAGTENTSEYG
                     STMAMDHVEYKFKGQPAHAAAAPEKGISALDAVEIMNVAVNYLREHLIQEMRIHYVIT
                     DGGAAPNIVPATAASRYFIRGPEYPDVAYARQRIDDCAKAAALATGAQLEIGFSSGIY
                     NKVPNKTLALKAIDAIDFVGTPKFNEDDIAKMAALGIAGVPSGAITEPTGSQSFGSDP
                     IGDVTWKTPATTIGIATWVPGTAGHSVEAALQSGSVYGFKGAAAASKALAVLGLELMT
                     NPDSLAAIVAEYEDRMKDMPPYEGKAMIPEVAYPEAPGVVVSASKGCVLLTSAETAFE
                     EAEGDVIAVKSASGEVFGEFLVGAKPLDAAAEYSFALSGKIEPGQRLKIFYTAAGTNS
                     TWFYGYVHAE"
     gene            complement(2097..3944)
                     /locus_tag="PLU15_01735"
     CDS             complement(2097..3944)
                     /locus_tag="PLU15_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOO29675.1"
                     /translation="MQKHAIWHVPYDAMAYPISDDRLEIVLRAARGDLRRVEAVYTDR
                     YTPHARTSSTRLWIAGSDGLFDYWRGSMKLQYGRFMYYFELQDDEKRICYGEGGFSDE
                     PPQDEPWTWCFQYPCIWYHGGMDDPPKWAQDAVVYQIFVDRFANGDRANDPPGVSRWG
                     TLPTPRSFSGGDLAGIIEKLDYISSLGVSCLYLTPIFLSSSNHKYDTIDYYHIDPHFG
                     NEDTARHLVDQCHARGIRVVLDAVFNHSGYDFFAFRDVVKKGEDSPYWKWFNIHGYPV
                     RTRPKPNYETFGPGIWQMPKFRTDNPEVRDYLISAAEHWTRTLDIDGWRLDVADDVDQ
                     GFWREFRTRMRQIKPDILIVGEILHDASPFLRGDQMDSVMNYPWRELCLDYFARRRIG
                     TADFADGITATAMHYRRQTQYAMWNLLGSHDRQRFLTACRGDTARLMLATTFQFTYTG
                     TPYIYYGDEIGLKGGEDPDCRKCMPWEEDLWDLDLLEHHRALARLRAEHPVLRRGDFA
                     SLIADGPDSPYVYARWDDDACIVVALNNTEEPQTVNTKLVAERMRKTHELEQWAGMPM
                     KQIFSLHGDNTGQGRSDNTQASMSAVGENCGRHVVGLPGMGAAVFQVQS"
     gene            complement(3982..4839)
                     /locus_tag="PLU15_01740"
     CDS             complement(3982..4839)
                     /locus_tag="PLU15_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29676.1"
                     /translation="MYKGFLEPAYLWRHIVIWLCIAFAILPVLWIISASFDPSNTIIG
                     QKLIPSNPSLENYRKLFNDPTHPFGRWMLNTLKVSFITSALVVIMTSLAAYSFSRFRF
                     RGRKTGLFSILLIQMFPQMLAMVAIYLLLLWIGEHFPTFGLNTHGGLILVYLGGAMGV
                     NVWLMKGYFDTIPTSLEEAATIDGATRFQCFYKVILPLSRPILVVIFFLQFIGTYSEY
                     VLAKVLLSSNDVLTLAVGLQSFIGDQYAKRWGVFSAAAIIGSAPILTLFALLQNELVS
                     GLTRGAVKE"
     gene            complement(4832..6409)
                     /gene="malF"
                     /locus_tag="PLU15_01745"
     CDS             complement(4832..6409)
                     /gene="malF"
                     /locus_tag="PLU15_01745"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HOO29677.1"
                     /translation="MKVATRRAPVDIPIPGLIAKVALLGILNALAIWAIFVLIGESAY
                     WIAVMLAAGCALIDWVFLTPRLFPWRYLIPGLIFMAAMVVYPICYTVAVAFTNYGTGH
                     LLTKEQVIENYLSRATLQEDAPEYPAAMYKSHDGRFAVVLIDPKGEKLLGVDGEVLPL
                     GEVQLPVEYADSDGVPESIGPYQRLGTLELFQHMSAVEALQFHFEDFILKMRNVDRFA
                     TYKPQYSYDSNLDALIDTTTGKVYTATQGTFLADDGEALDVGYRAPVGWSNFARLIRS
                     PQVSGPFFRVFVWTFEWAALSVVTTFILGLLLAVLLNDQKMKFKGFYRVLLVIPYAMP
                     AFISALIWRGLLNAEVGLINDMLQFLFNVRVPWLQDPFWAKVALIIVNLWLGFPYMML
                     VSLGSLQSIPDELYEASRVDGATGWQQFWHVTFPLLLVSVAPLLVSSFAFNFNNFGVI
                     YLVTQGRPAIPGAQTPAGATDILITYTYRLAFESGSGTDYGLASAVTILIFLIIGTLS
                     WFNFRFTGALEEVHENV"
     gene            complement(6519..7724)
                     /gene="malE"
                     /locus_tag="PLU15_01750"
     CDS             complement(6519..7724)
                     /gene="malE"
                     /locus_tag="PLU15_01750"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF007011.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HOO29678.1"
                     /translation="MKRSLAIAMCISMLLGLATCASASQEGKLLIWADGTRAPVMEDL
                     GKKFTAKYKVPVEVQELGFGDIRDQLGVAGPAGKGPDIIIGAHDWLGELVTNGLIEPI
                     DLQAKEKDFLKVSVEAFTWGSTLYGVPYAVEAVGLFYNTKLVPNPPKTWDDLVAICKK
                     LTDKDKKQYGFLLPIPDPYHTFPIMSAAGGYVFGVNKDGTLNPLDVGLNNAGAVRGLS
                     LLDKLVEDGLMPQSMDYNTMMGLFKDGKVGMMLTGPWALADLRAAKVPYGFTTIPKID
                     GKDPRPFVGVQGFMISSFSKNKMLAKAFLNEYVATADTMMALFKQDPRPPAYLATARA
                     VSSDADIRATGDSAAAGIPMPAIPEMSAVWTAWANAIELTFNQKLEPKKALDDATAQI
                     ISAIKASKK"
     gene            8104..8676
                     /locus_tag="PLU15_01755"
                     /pseudo
     CDS             8104..8676
                     /locus_tag="PLU15_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(9451..10056)
                     /locus_tag="PLU15_01760"
     CDS             complement(9451..10056)
                     /locus_tag="PLU15_01760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO29679.1"
                     /translation="MRIYLVRHGQTVRGREETFRGRMDAPLDDMGRKQAAAVATALRD
                     VKLVAVYTSPLSRSVDTARAIAVSHGINIIVRDALTDIDVGEWEGLSTDRIRSDYGRL
                     YLDWQTGPDSFTFPGGESLSDVSDRAMAAMDKIVSSHEHDDELAIVSHRVLTKLILLN
                     MLHAPVSSFWKLSQDPCCINVVDYTEAGYVVESVNCISHLA"
     gene            complement(10053..11438)
                     /locus_tag="PLU15_01765"
     CDS             complement(10053..11438)
                     /locus_tag="PLU15_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOO29680.1"
                     /translation="MTENMDALNGFFHRFHRAKLNPSQVLVTGFAAVIIIGAILLSLP
                     AASANGKSIRFIDAIFTATSATCVTGLVVLDTATSFSLFGQIVIIILIQIGGLGFMAL
                     ATAIALFLGRKITLRGRILLQESLNQFSIAGLVRLTRYLLLTTFIVEGVGAIILSLRF
                     SSIMPTSKAIYYGIFHSISAFCNAGFDLFGSITGPFSSLTYWASDPTVVLVIGSLIII
                     GGLGFPVIVELVGHRRGNRLSLHARLVLTVTLWLLAIGTAATFGLEFSNPDTLGKMSP
                     AGKVLGAVFQGITPRTAGFNTLDISKMRSSTLFIFVILMFIGASPSSTGGGIKTTTMG
                     VIIAAILSTIRGSDDVEINDRQISQSLVFRAITIASLALALVTGVTLVLSATEEASFL
                     QVLFEAASAFGTVGLTTGITPNLTDFGRVLIMITMFFGRLGPLTVMLALAQRQKPLAN
                     VHLPEEKVLIG"
     gene            11699..13003
                     /locus_tag="PLU15_01770"
     CDS             11699..13003
                     /locus_tag="PLU15_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOO29681.1"
                     /translation="MDDPSSSTYLFAFICLVLSGAFSASEAAILSSDEARLKSRYSSD
                     PRLRSILAVNKSPAPVMSSILLGNTLVNAMFSALAAFIIHSIVPGDAGLADAIATAAA
                     TVMLLVFAEVSPKLVSGGNPEAVALSASSWLLGLARALAPVSSVLTALSGHLVACLPQ
                     PDTAEEDFGELRFLAAVEYGEEAGAISGLEKEMIWGAMETHDLLAKDIMTPRPRIVAA
                     SEDQSTAEALDLMLKYGFSRIPVYADSIDNVTGILNMKDIALLISCSPNDWRERLDLM
                     PCVQLAVRPYFTHERKRVSDLLIEMRLKGIHTAIVVDGHDGVSGLVTLEDALEVIVGD
                     IQDEYDEETPELVMSEAGCCHVVGSVRLADLAEAFDVELPTEEFDSVADFIMACLDRV
                     PSEGDAYSLVGPRLTLEVVEMDGPRIKRAIVRLSEMETGRHG"
     gene            12996..14084
                     /locus_tag="PLU15_01775"
     CDS             12996..14084
                     /locus_tag="PLU15_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOO29682.1"
                     /translation="MGELDLLVSLMIRTILFVGSVFGVGFFAASESAFLSMNKWAIDK
                     LAEEGDKNALIMRRLTEDSAKTVSAVLVGTNVLSTLSSVLAVSILLLVGKSGTRAIAI
                     ASIVTTAIILIFSELTPKTYAAANPTATALKVAVPLSWAITLLGPAASFLAWVPSQIA
                     ALFRRKSNAGNESPDEIIRAALDAAEDDGSVVPTETEVIHGVLDSKRKFVRDIMIPID
                     RTVALDYDSTLEDFMEAYLKERYSRMPIIASFCGKVLGIAHVKDVMKEIVAGGDPAAK
                     KVQSVANTACLVSADAPALDAFNEIRSRKAHLAVVVQRGIAVGIVTIEDFLEEIVGDI
                     PEDASGHVVKSTYGTMALAVTVEGAENA"
     gene            14127..14573
                     /locus_tag="PLU15_01780"
     CDS             14127..14573
                     /locus_tag="PLU15_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29683.1"
                     /translation="MQRCKRAVNTVIKTWCPLSRDDRTFIARLIVAEALCGLCKELGC
                     DTGICVFSKKEAEHSIANVPSELSDIGPMYAAFVLFSEAFYFGENGLAVLFPALRRPE
                     WLRSRYIFFRSRIPERALLETDRIAMEYTLYASGDRLNVEAPGGLI"
     gene            14574..14966
                     /locus_tag="PLU15_01785"
     CDS             14574..14966
                     /locus_tag="PLU15_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29684.1"
                     /translation="MRLCLAPTVLEETSANGVIVADVTIGELVYDGKLLDALEELDAP
                     VRFIVEEGMFAERLRNLLWTLPRSSRHVVELGIGLSNITPTGYIGADECIAGTCHFGI
                     GDNRFYGGRNASPIHLDVVVDRPRIKRY"
     gene            15205..15281
                     /locus_tag="PLU15_01790"
     tRNA            15205..15281
                     /locus_tag="PLU15_01790"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15239..15241,aa:His,seq:gtg)
     gene            15373..15449
                     /locus_tag="PLU15_01795"
     tRNA            15373..15449
                     /locus_tag="PLU15_01795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15407..15409,aa:Arg,seq:tcg)
     gene            15626..17128
                     /gene="tig"
                     /locus_tag="PLU15_01800"
     CDS             15626..17128
                     /gene="tig"
                     /locus_tag="PLU15_01800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOO29685.1"
                     /translation="MYKASVVDNDQGLVTLEITVSEDEVRRALGSAVRRLGQRYTVPG
                     FRKGKAPRLILENYVGREIIYDEAIRDFVPKAYEAALEETNLIPLEDPAFEGLDDADL
                     DSGEPLTFKARMTVKPEVKLGDYKSIKLDREKREVTEADIDRVLENLRDQRGEFVSTE
                     RTTAQAGDLVTVSIEAMVDGERLEEISVDDTQVPVGMGYLMEGIDEEIEGMTVGEPKS
                     VTAVLPEGFYIEELAGHDVTFNIIVKDIKVKQLPELDDEFAKDFGETKTLEELRNDIK
                     HSMMHHFLDEAKKDLGERALDALVTTSEVEPPKLLVEQEAYIAFDKLQETVEKEGLEW
                     EEYLEAKGQSEDDVYQEMLELAPASVKKALVIEALAKEENLFPTTDEVSIAVAQVMSY
                     AGAQSREILQKAIEDPEMRRVTTKALMREKVLAYLANTCDADPESAICQECAAREAEE
                     AKENAEKAQAESGTESRDEGIEQTSEADEPGSDEEMDGEAVAEETSSPEP"
     gene            17366..17959
                     /gene="clpP"
                     /locus_tag="PLU15_01805"
     CDS             17366..17959
                     /gene="clpP"
                     /locus_tag="PLU15_01805"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOO29686.1"
                     /translation="MTLIPMVVEQTSRGERAYDIYSRLLKDRIIFVGGPIDDSMANLV
                     IAQMLFLQGEDPEKDINLYINSPGGVVTAGLAIYDTMQYVKPDVSTVCVGLAASAAAV
                     LLAAGAKGKRYALPYSRIMVHQPHGGTQGQATDIEIQAKEILRIKGITNEILSRHTGQ
                     PIERIDRDTDRDFYMSAQEAKEYGIIDSIFEGKAAEA"
     gene            17975..19240
                     /gene="clpX"
                     /locus_tag="PLU15_01810"
     CDS             17975..19240
                     /gene="clpX"
                     /locus_tag="PLU15_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOO29687.1"
                     /translation="MFKYGDDKGQLRCSFCGKAQEQVRRLIAGPGVYICDECIELCNE
                     IIEEESGISPDIDFSEIPKPHEIRAILDEYVIGQDDAKKTLSVAVYNHYKRISAAPHV
                     DDVELQKSNILLLGPTGCGKTLLAQTLAKILNVPFAISDATSLTEAGYVGEDVENILL
                     KLIQNADYDIERAEKGIVYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFIQLDTTNILFICGGAFDGLDSIIENRIGRKTLGFGAEVKPKVDK
                     NIGDILRQVMPEDLLKYGLIPEFIGRLPVVVPLDALDEESLVRILTEPRNALVKQYRK
                     LFELDGVELEFTDDSLEAVARKAMERNTGARGLRSIMEHIMLDIMYDIPTRTDVKKVV
                     ITKETVADDGKPTMVLAEKKPARKKEETA"
     gene            19379..21121
                     /gene="lonB"
                     /locus_tag="PLU15_01815"
     CDS             19379..21121
                     /gene="lonB"
                     /locus_tag="PLU15_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOO29688.1"
                     /translation="MSNLGVIGLINLFFGIVIGLYFWNLLKVQQGSKTAVDRDSRREM
                     DKLREMRSLSLTRPLTELTRPKRFEDVVGQKDALLALKAALCGPNPQHVILYGPPGVG
                     KTAAARVVLEEAKKSPLSPFRADAVFIEIDANTARFDDRGIADPLIGSVHDPIYQGAG
                     PLGIAGVPQPKPGAVTKAHGGVLFIDEIGELHPIQMNKLLKVLEDRKVFLESAYYSPD
                     DRNIPMYIHDIFKNGLPADFRLVGATTRPAHDIPPAIRSRCLEIYFKALTPEEVGIIA
                     SQAVDKMNMRVEDGVIQIVTKYAQNGRDAVNMVQLAAGVAITEGRKMVMRSDMEWVVE
                     SGQYSPRPERKVPDAPQIGIVNGLAVWGPGMGSVIQVEASAVPAEAGTGSFIVTGIVE
                     EEEQGDGSKILRRRSMARSSVDNVSTVLRNRHALQVGDYHIHVNFPGGAPVDGPSAGI
                     AMATAVFSAARQIPIDNRVAMTGELSVHGKVKPVGGIVAKVEAAVSAGATRVLIPAEN
                     WNERFADRADVQVIAVEDIDEVLRIAASCNAAETESEATCTPWPEVAAAASCRRGDAP
                     GGTGSMPPTVQITG"
     gene            21775..24255
                     /gene="lon"
                     /locus_tag="PLU15_01820"
     CDS             21775..24255
                     /gene="lon"
                     /locus_tag="PLU15_01820"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOO29689.1"
                     /translation="MARADKLSHKDRENVAQSLPLLPLRGVVVFPNTTLPLEVGREKS
                     VAALEEAMSCGKLIVLAAQKVAKVNEPSPDDIYTVGTVSAIKQLVKLPDGTIRVVAEG
                     VERAVIHEYVQSDPCYMVQVELMPTVEVCDREIEALARNALREFEQFVKLSKKMPVEI
                     MLAIGGTDEPGRLADDIAAHMPLSVEDKQELLSCFSVRTRLERLAVILRRELEILELE
                     RKIHLRVRKQMEKTQKEYYLREQMRAIQKELGDRDDPSSEAAELRQKIEEANFPPEVL
                     EKAVRELDRLESMPPMAAESVVVRNYLDWLLAVPWGVMTEDRLDIERAEAILDEDHWG
                     LDKVKDRILEFLAVRQLSDELRGPILCLVGPPGVGKTSLGKSVARALNRNFVRFSLGG
                     VRDEAEIRGHRRTYVGAMPGKIVQLLKQAGSMNPVILLDEIDKMSADFRGDPSSALLE
                     VLDPEQNSEFGDHYMEVTVDLSNVLFITTANNLYSIPRPLQDRMEIITLPGYTEEDKV
                     QIATHFLIRKQLSENGLSEENVVIQEDAVREIIRCYTRESGVRSLERQIGAICRKIAR
                     EVVAGAEGPFVVGRADLQEYLGVPQYRYGVADLDSKVGVAVGVAWTETGGDTLDIEVS
                     AMKGSGKLILTGKLGDVMRESAQAGYTYVRSRARELGIPEDFYEKMDVHIHVPEGAIP
                     KDGPSAGITMATALASALSGVPVRNDVAMTGEITLRGRVLPVGGLKEKTLAAHRAGIR
                     TMIIPDDNKKDLEELPENVAGDMTFIEVSHMDEVLAIALLSAKDDEKSSFGADYAVGE
                     AGDEQGGTMRDVPLPHVVPNTPQPPAVC"
     gene            24622..25389
                     /locus_tag="PLU15_01825"
     CDS             24622..25389
                     /locus_tag="PLU15_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO29690.1"
                     /translation="MTSTEPMKPAGALYIGIASQLKNDIVRGAFPDNRLPTEEELSQR
                     FGVSRNTIREALSMLEMDGLITRRQGVGTFIERDKVHVGSRTIGFTESMRLAGRNPGT
                     LMMSFDWEPADQHMSSMLDMRVGEAMGVLKRVRSLDGVPAYYQIERLPAHVIGEDFDP
                     DAMGESLFEYLNKQRDIEVGGSEFSLRATVPNPVIAGMLGMDIGLPLLMIEDLYRSRE
                     DKPLLWSVNYYRSDLYCFRAVERNGTFEMLTVQEEKI"
     gene            25407..26297
                     /locus_tag="PLU15_01830"
     CDS             25407..26297
                     /locus_tag="PLU15_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOO29691.1"
                     /translation="MQQDFRYPKTAEDVTAEMLAMPPEELEKVSRYPLYIHKTRDDLY
                     KWIARLMADELKENNAKGEPTRWVLPIGPKGQYPLLAKICNEERISWKNVWAFHMDEY
                     LDWQGRPISYDSPFSFRRYADEHLYKLIDDELLPPKEQIIFPDVYDIDAFTAKIDEVG
                     GVDSTFAGFGYRGHLAFNESPCNRWQKISADEFAAGKTRVVHLLDDTIIALSQRMLGG
                     HSDPIPQMAITIGMADILRSSKIYLLTDGAGWKQWMLRVFLLTTERDPRYAVTLCHGH
                     NGVIVGVDAASAAPITLGIV"
     gene            26323..27285
                     /locus_tag="PLU15_01835"
     CDS             26323..27285
                     /locus_tag="PLU15_01835"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOO29692.1"
                     /translation="MTRHTKKTAPTITVVGSFTVDMMMRIPRLPVYGETLLGSSFLQA
                     PGGKGANQAVACARLGANVNYVGVLGMDSMAEAALELFRQEGIGTECLFRTDEAATGV
                     GFVLQFPDGNNVCLTDMAANQHLSADMVDRAASLIEESDAVLTCLEIPTDAALRSMEL
                     ARKHGVCSILNPAPAIAGIEKLKGHVDVLTPNHVEAAALLNIDPDAATSADAVAIART
                     LREEGFPAVIITCGEDGAVIADGCGEMKVLGAKVQAVDTTGAGDTFNAALAVAIAEGK
                     PLAEAVMFANCAGALACMKVGCVAAIPYRDEVEERFRSTYSAMN"
     gene            27323..28798
                     /locus_tag="PLU15_01840"
     CDS             27323..28798
                     /locus_tag="PLU15_01840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO29693.1"
                     /translation="MRQICKRFGGTLALDNVDFAVSKGEVHALIGENGAGKSTLIKIL
                     SGIYSADSGTIAVDGETVEIHSPQDAQRLGIATVHQELNLAPDLTVAENVYLGRLPTK
                     RGSIIDWSALKNEAELAFEQLGVADQIDPMAPVRSLSISKQQLVEIAKAFSAGARLII
                     MDEPTSSLPESEVQHLFDVVRTLNRKGVSFIYISHRLEEVLAITDSMTVLRDGRLVGS
                     CKTAETSYDDIVRMMVGRELTERFPKREPSIGETVLEVEGLADAGRFRDISFQVRAGE
                     ILGISGLMGSGRTELAQAIFGVVPPEEGSIRLNGKELNVRCAEDGIEVGIYLVPEDRK
                     RHGLVQTMSVRSNTVLSILSRLSGAFGVVDKNKEEQVVTKAVRDFNVKTASIDQNVSS
                     LSGGNQQKVVFAKAVCSGPKVLILDEPTRGVDVGAKAEIYKLVQDFASSGGAVVFISS
                     EMPEILGMCDRILVMSEGRIVGEFAGSEATQEEILKCALAK"
     gene            28819..29775
                     /gene="rbsC"
                     /locus_tag="PLU15_01845"
     CDS             28819..29775
                     /gene="rbsC"
                     /locus_tag="PLU15_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007440333.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOO29694.1"
                     /translation="MMRRGVMQKYRILFVFVGICILLSVLTPVFLSVNNIINVIRQVS
                     INGILAAGMTMVIISGGIDLSVGSVAAICGAIVAGTQLKLGLVPAMLLSVATGALLGL
                     INGLVITKGKVAPFVATLGMTTVARGLTLIYTGGRPIYNLTDAFRVLGAGYVGPIPVP
                     VLILALVIAVVHFVMSSTVFGREVYALGGNEEAARYSGINVERRRMLVYISMGLLSAV
                     TGIVLTSRLGSADPTAGVGFETDAIAAVVIGGTSMSGGEGSVIGSLIGALIIGVMNNG
                     LNLLNVSPYYQQIFKGLIVVLAVLMDSASRRKRQAAALKVGR"
     gene            29885..30823
                     /locus_tag="PLU15_01850"
     CDS             29885..30823
                     /locus_tag="PLU15_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO29695.1"
                     /translation="MRFSTKRTTQVVIGIIMVALCMVMNVGGAAVAAAAKPRTVAVAF
                     CTLNIPFFVEMENGIRAAAAKNNVKLIVTDAQDSAAKQLSQVEDLLVQKPDLLLITPV
                     DSDVLVPAVEAAYKAKVPVITIDRSVNSDKVVSFIGVDDVEGGRMAGRYVVERLKGKG
                     NVVMLEGVMGSSAQRDRAQGFEEVIGKNKGIKMLAKQTANFNRGEAMTVMENWLQSFR
                     TIDAVFAQNDEMALGAIEAIRAAGRQNEIIVVGFDADPGAKQAVKEGKMAATIEGSPY
                     KLGVMGIQAAVDCLAGKKLPKQILIPLDLITKDTFK"
     gene            31255..32577
                     /locus_tag="PLU15_01855"
     CDS             31255..32577
                     /locus_tag="PLU15_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOO29696.1"
                     /translation="MEKTKVIIMGAAGRDFHNFNVYFRDNERYEVVAFTATQIPDIAG
                     RMYPAELAGKLYPNGIPIYPEDQLEELIVKHDIDQAIFAYSDLSHVDIMHKASAVMAA
                     GADFRLMGPNTTMLKSRVPIVAIVAVRTGVGKSQTTRYVSQALIDAGLKVVAIRHPMP
                     YGDLAKQAVQRFATYEDLDIHECTIEEREEYEPHIDRGVIVYAGVDYGRILEEAEKEA
                     DVILWDGGNNDFSFYKPDLTITLVDPHRAGHEVLYHPGETNVRLADVVIINKVDTAKP
                     EDVETVRKNVQRINPRAQIIDAASPVTVEDPEMIRGKRVLVVEDGPTLTHGGMGYGAG
                     VVAARQFGAGELVDPRPFAVGSIVDTLERFHHLEPLLPAMGYSKKQIDELETTINRSD
                     AEVVLIGTPIDLRKVLKIDKPAVRVRYELEQESGPDLRDIVCSKFAKE"
     gene            32730..33968
                     /locus_tag="PLU15_01860"
     CDS             32730..33968
                     /locus_tag="PLU15_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29697.1"
                     /translation="MRRSSCATRHKAFPCVLAFTMAVFFCPPWQAAGVSAANDSLVLK
                     VALDSESLPKLTATLSHKAARFSSTVKSSTEGTHEFKLEAKMAEDISHLRQSATLSIS
                     GLLAAQGDASCWRQLSSTTGVVVDRKASPWRPEGKLSITVREYPLNAARDYSEGTVRV
                     KAGRTWSQPYAVALKAEATAGHKEMPGNPKWTANYWESSATAQSPLGSECKGSITVSA
                     KGRAYPEAEHKSYTRASCKLEGVWTPSKVHSIDCSLTAAATVREKDRSCDKTDTAISA
                     RWSIEPSRHWSADIKGSLSSSIEPNRADAGRPVRATGGVTLTCVPADIWRLTAAGDLS
                     LGHTLGENEDGDEEIVGIQSGQSGKLKMGLSFAAKLGPVNGVTLDGKLNLKRESEHAK
                     DRWEHSTWAPSAEIQASMKF"
     gene            34075..36162
                     /gene="fusA"
                     /locus_tag="PLU15_01865"
     CDS             34075..36162
                     /gene="fusA"
                     /locus_tag="PLU15_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO29698.1"
                     /translation="MKKYKVDQLRNLAVVSHGGAGKTTLAEAMLYLSGGVDRFGKVDE
                     GTSTMDYDPDEVKRKISINASVAPVEWKDHKINFIDTPGYSDFVGEVVGALRVADSVL
                     VAVDAVSGVEVGTELMWRRVEEYDLPRMIVISRMDRENANFNKAVESLTEIFGNRVAV
                     AQIPIGEGDKFRGVVDLIKMKAYVWEDASGKKFVETDIPADLADIAEEHREKLIEFAA
                     ESDEELLDKYLEGEALTEDDIKAGLAKAVLSRSAYPVFCISGMKLIGIQPLMDAIISL
                     CPSPVAVGPVKGIVPNSEDTIERKPEETEPFSALVFKTMADPFVGKITMFRVYSGVVK
                     SDSQVYNASRDKTERFGQIFLTKGKQQVAVSEVGPGDIAGVAKLADTTTGDTLADKDN
                     PIILPKIQFPSSVLSMAVKPKSKGDEDKISQGLARLMEEDPTLKYDKDAESSELVVSG
                     MGEVHLDIMADRLKRKFGVEVTLEEPKIPYRETIKNTVKVEGKHKKQTGGRGQYGHVW
                     IEMSPLPAGSGFEFEDKVFGGAVPRQFIPAVEKGLREALPEGGVLAGFPLIDIKCSLY
                     DGSSHPVDSSEMAFKIAAQLALKKGCQDANPIILEPILKVEVTVPEEYMGDVMGDFNK
                     KRGRILGMEPEGRYQVVKALVPMSEMAKYSIDLRAMTGGRATFTTEFSHYEEAPANIA
                     ARVIEEAKKEKEA"
     misc_binding    36215..36553
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            36644..39304
                     /locus_tag="PLU15_01870"
     CDS             36644..39304
                     /locus_tag="PLU15_01870"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOO29699.1"
                     /translation="MGTTEISTTYNPKEIEEKTYQFWLERGYFHAEPDPDPEREHFVI
                     LMPPPNITGTLHIGHALDVTIPDILIRWKRMQGANVCWIPGTDHASIATEAKVVASLA
                     KEGLSKTDIGRERFLEIAWEWKEKYGGEIVEQQKKLGASCDWDRKRFTMDPVCSKAVR
                     EVFVRLFEKGLIYRGDRMINWCPNCRTSLSDVEVEHEDVESALWHLRYPLADGSGHVV
                     VATTRPETMLGDTAVAVNPKDPRYAKLVGKTLILPIMDREIPIVADAHVDPEFGTGAV
                     KVTPAHDPDDFEIGERHGLASVKVIGPDGIMTEEAGKYAGMTREECRRQVVEELKSAG
                     LLEKIESYEHAVGHCQRCGTAVEPMVSRQWFVKMKPLAEPAIEAAKDGRVRFVPERFT
                     KTYLNWMENVRDWCISRQLWWGHRIPVWYCTSCDHMFASREDATSCPKCGGQVEQDPD
                     VLDTWFSSALWSFSTMGWPEETPELKSWHPTSVLVTGYDIIFFWVARMIFMSLEFMGE
                     EPFSDVLLHGLIRNPDGSKMSRSKGTGVNPLEIIEEYGADTLRFTLITGNTPGNDIRW
                     RPEKVEASRNFCNKIWNASRFVMMNLEDFTLPEGKVMPEDLELNLADRWIISRMNRVA
                     ADITEQLGRYDMGEAARTIYDFVWGEYCDWYIEIAKRRLYGEDDPVGRRTAQYVLWKV
                     LDGSLKMLHPFMPFITEGIWQHLPGSGESIMIEPWPTADKDEIDAVAEAEMAVLMETV
                     RAIRNIRAEFNVEPGKKVDAIFHADGEAFDTINVNVDIVAHLAGLASVTVEHVSAAKP
                     EKAAAAVVPGIEVYLPLAGMVDIGKETNRLRREEENLLREVESARAKLSNEGFVTKAP
                     AEVVQKQRDRLGEYEEQLVKIRARIAQLEG"
     gene            39308..40117
                     /gene="nth"
                     /locus_tag="PLU15_01875"
     CDS             39308..40117
                     /gene="nth"
                     /locus_tag="PLU15_01875"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO29700.1"
                     /translation="MMSDRHTDEVGVPMDVAGGEGWVRAPSSVSSEIVRRVDEALRHM
                     YGPKMLAPNGDPVGELIHIMLTQNTSDANSDRTYAALRSAYSSWEAVAAASPSEIAEV
                     IRMGGLADIKAARIIEVLSRIEADYGCISLDAIDSMNDDEAFDYLTSLPGVGPKTAAC
                     VLLFALGRPVFPVDTHVHRVANRIGLVDTKQPAKTQEELGAVVPEDIVYQLHMNMVAH
                     GRRTCRARRPKCEECALRDECAWAIAASCKAGDKGVSEESVAERVAEGGRA"
     gene            40184..41497
                     /locus_tag="PLU15_01880"
     CDS             40184..41497
                     /locus_tag="PLU15_01880"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOO29701.1"
                     /translation="MDYEAAVDYLRSLSRFGIKPGLERTEALLQSMGNPERSMAHVHI
                     AGTNGKGSVSVMVESALEEAGHVSGLLTSPHLQRYTERIKVGGREISQQQLAELVGEM
                     APVIAKLSENENIGTPTEFEALTAACFEWFMRSGVEIAVVETGLGGTYDSTNVVTPEV
                     SVITHIAMDHMDRLGNTLSEIASNKAGIIKPGVPVVVSPQDPEAMAVIVAKANEVGAE
                     VTVVGMDVPYEPIHMDLDGTTVDVDAPVLGRLRVHTPLVGRHQVTNCATAVAALDVLE
                     GLKGGTISPAAVCSGIGKACHPGRFEVVRKSPYVVILDGAHNLDGAASLAATLHEIAY
                     GLRPRVMVFGTSRDKPVREMLAALVPEVDVVIATAAVGARTGAHDPEFVASVAREMGV
                     EAHAVASACEAVQAGARTASAIGAKSLLVAGSLYLVGEVRGIWRD"
     gene            41500..42234
                     /locus_tag="PLU15_01885"
     CDS             41500..42234
                     /locus_tag="PLU15_01885"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOO29702.1"
                     /translation="MTQGWAEMEKFRKARVTVLVGAFGSGKTELALNFAKRLNAAGRN
                     AVLVDIDILKPMFRSRDKRNDLSELGVRVVSSVTDYEDSDVPALSPAIYGVIEDRSSD
                     VVVDVGGDDDGARALGRFREALEEAGYEMLYVVNTRRPFSSTPEEIVSMVLAVEEASR
                     LRCTALVSNTNLGSESFVGLVEEGLDITLQASQTLGVPVRFAMIQKSVADSQRNELEK
                     IVQVYGVPVFEMDRSILPPWEGIAQS"
     gene            42319..42918
                     /locus_tag="PLU15_01890"
     CDS             42319..42918
                     /locus_tag="PLU15_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOO29703.1"
                     /translation="MPAKRSSSGNALSWILAVVLISVAAVGVGWLIGQQVLGWINPNR
                     SSVSEEDSGDTGGYSYYPWSGSDGAGSASQQPQQTPAPNINLPTSSLPESSAVEPPTY
                     GAESSSVPSSVPDGIGSTQGTVSSPVAAEFYKVRVGSFGTKEAAAATARELEAQGYPI
                     FVTGGGPWSVQVGAFSLRENAIVLGDILANQGYDVTIVE"
     gene            43160..43543
                     /locus_tag="PLU15_01895"
     CDS             43160..43543
                     /locus_tag="PLU15_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOO29704.1"
                     /translation="MKLADIRELIGAEVLVGEDKLSTVEIEMACGSDLISDVLAFCHD
                     KTLLCTGLVSAQVVRTAEIAGLSTIIFVRGKRPDESVIEMGKAAGIPMLATDLPMFET
                     CGLLYGAGVGGICLGADGSRRGDVG"
     gene            43636..44043
                     /locus_tag="PLU15_01900"
     CDS             43636..44043
                     /locus_tag="PLU15_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29705.1"
                     /translation="MSFRVDRYDFAAAGEAASKIKQVLRQLGMAPELVRRAAVAAYEA
                     EMNLVIHSEGGQILLRVDTEKVTLVACDGGPGMPDVSLAMREGYSTAPDEIREMGFGA
                     GMGLPNMKRCSDSFSIESEVGRGTTVTAAIRIA"
     gene            44096..45436
                     /locus_tag="PLU15_01905"
     CDS             44096..45436
                     /locus_tag="PLU15_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOO29706.1"
                     /translation="MGMEGRFHSVKLLEEKCKGCTNCIKRCPTEAIRVREGKAVINAA
                     RCIDCGECVRTCENHAKTIIANSIDDLKNYDYTIALPAPALYSQLGRASGPDEVVWAL
                     QEIGFDAVCDVSFGADIVTECTRRHLISHPKPRPVISAACPAVVRLIQVRYPNLISHV
                     ARVESPMYAAARVARVEAAEKLGIAGDRIGVFFISPCAAKVTAVIAPLGSEFSYVDGA
                     ISISDIYGELMTELGRGRSARPMHRGSNLGFAWANSSGELKALGLHNVLAVDGIHNVL
                     GLLEEVDNGRLAGVDYIEALACPAGCVGGPATVENPFVARARMIEISKAVEADSAEAL
                     SEARRLVDERGTDFFALQGELTALSGLEIDPRLEVAIAKMSELERVLARLPGLDCGAC
                     GSPTCRAHAEDVVCGNASEVDCVFKLREAMEEVAEELLRLAKTDLPSLQRRNDK"
     gene            45433..45777
                     /locus_tag="PLU15_01910"
     CDS             45433..45777
                     /locus_tag="PLU15_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOO29707.1"
                     /translation="MTVREIAAVLGMKCVAGGVGLDREVSSGYVSDLLSDVMANASDG
                     GIWVTSQVHQNVVAVALLLDLAAVVIAGGLEMNEDAIARAEAEGMPILSTKMSAFETV
                     GRLYELGVRGDS"
     gene            45780..46523
                     /locus_tag="PLU15_01915"
     CDS             45780..46523
                     /locus_tag="PLU15_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO29708.1"
                     /translation="MVQGFAADLHIHTFLSPCAGREMNPKAIVGRCLDLGLDIIASAD
                     HNSAANVEALAAAAEGTGLTVICGMEVCTKEEVHLLTLLPDLSAMHEWELIVAPHRAT
                     GRNIPEIFGRQWICSPRTGDITEETALLSAPTTLSVDEVVDGVDALGGISVPCHIDRR
                     AFSIVAQLGFVPDHLDIPVLEISRTITATEARAKFPATQGRVLLSSSDAHTLEEIGLG
                     CSLFWLEEPTFAELILALRGEAGRYVQVE"
     gene            46600..47127
                     /locus_tag="PLU15_01920"
     CDS             46600..47127
                     /locus_tag="PLU15_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29709.1"
                     /translation="MHILDIAQNSIAAGAELVEIDVIEDVANDKMIIRVADDGRGMPA
                     DVVAQIRDPFVTSRTTRRVGLGIPLLAAAAERCGGGVDIASVPGGGTTVTAIFEYSHV
                     DRAPLGNMAETLLALVVCNPNLDFVYNHVRGDNSFHFDTREIRLELGSIPLSDPEVVV
                     FIRDFVAEGERRLDA"
     gene            47124..47612
                     /locus_tag="PLU15_01925"
     CDS             47124..47612
                     /locus_tag="PLU15_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOO29710.1"
                     /translation="MRQFGIVKRVCGESVEVLVSRPAACKGCGACQMANNRPHTVIAR
                     NDASAEVGDTVALELPGQDLMRAVLLAYGVPLVMFLAGLGVGALAARSFGWTIDSSLA
                     SAIGGILFLAAGYYGIHLYDRSLGTEAFASVAVAVVDSNNPECASIHSGAGRVEDTEE
                     TE"
     gene            47609..48274
                     /locus_tag="PLU15_01930"
     CDS             47609..48274
                     /locus_tag="PLU15_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOO29711.1"
                     /translation="MKQEKIPDVVVRRLPLYLRAVEDFDRREHAIVSSSQLGEWTGLT
                     PAQVRKDLAFFGEFGKQGIGYNVKFLHEQLREILGLTTDVRVGLVGLGNLGRALLHHN
                     VDARIDGSAVEGDPARWVPESLRIVAAFDVDPQKVGITYAQVPVYHVDEFAAKVRELD
                     LKIVVIAVTPESAGAVASECVKAGVKAILNFAPVKLDVPEDVKVHSADLTLELQSLAF
                     YTR"
     assembly_gap    48306..48405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48876..50219
                     /locus_tag="PLU15_01935"
     CDS             48876..50219
                     /locus_tag="PLU15_01935"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOO29712.1"
                     /translation="MELNQELMIGSVYARDAIHSLYGGQRGLRISTPKAAPVVLLFAR
                     KSCDFKVRHGVDCGSPEGEHSCALPEDELPIAHTASAELAPYVSDPEEGWRDDGFYHF
                     RGEKHVQFLRGNRAVRDHARDGKLLFLFEETTRSEGRQAWRFIGSMECAGYYFAQASA
                     GRMAIMFRLAPVHAIDRLRKNSHELVLHSVLAERCALDFLNSRHGAIAASAESTRPGH
                     HSNAWKWSSDCALIQRYALDRAFGFCESCGKPSPFSDPDGEPYLEPHYMLRPYDSGVD
                     PVHGIAACCPECHRMIHDAWNGRSHDVALARKIALLERSVDRGDFLAVTAAVIRNAEG
                     KVLLTQRGHGALAGKWEFPGGKIEPGETLEACLAREIREELNLDIADIRPLVSSFYQY
                     DDSAVRLFGLSANAVGNQLELREHISKAWVWPHELESYDLASADLPIAAVAARLL"
     assembly_gap    50294..50393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            50531..51382
                     /locus_tag="PLU15_01940"
     CDS             50531..51382
                     /locus_tag="PLU15_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOO29713.1"
                     /translation="MFKIVEKCTLAPGVHQYEVEAPLVAKGARPGNFVVVRTSETGER
                     IPLTIAGHNRETGTITLVVQEVGKSTMEMGMLEVGDYFKDVAGPLGQATRIEKVGTVV
                     AVAGGIGVAPVLPQIRAHKAIGNHVISIVGARTRDLLVLRDEVEKFSDEVIWATDDGS
                     LGYHGFVSGALEELIGSGQKIDEVIAIGPVRMMQAVADVTRKHNIPTTVSLNAIMVDG
                     TGMCGCCRVTVDGEVKFSCVDGPEFDGHKVDFDELVSRQGFFRDKEQIARERFEADRG
                     GCKCHAK"
     gene            51397..52854
                     /gene="gltA"
                     /locus_tag="PLU15_01945"
     CDS             51397..52854
                     /gene="gltA"
                     /locus_tag="PLU15_01945"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOO29714.1"
                     /translation="MPKQSPEARRTNFSEVALGLDPELARREAERCIQCKTRPCTTGC
                     PVEVDIPEFVALIAEGKYDEAAAKIKEKNSLPAICGRVCPQESQCESLCTLGRKYEPV
                     AIGALERFAADWASKRAVEKREACAGDVDSDLQCGCEQKVCELDSRGCAPTSDSAASA
                     ARVAVIGTGPAGLTCAADLALKGYKVDMFESLHDTGGVLRYGIPEFRLPKSIVDEEVE
                     YVRSLGVRIFTNVVVGLTLDLREMLESGQYQAAFIATGAGLPYFLGIPGENLNGVYSA
                     NEFLTRVNLMKAYMFPQYDTPVNIGSKVAVVGAGNVAMDSARTSLRLGAEEVKIVYRR
                     SRAEMPARLEEIENAEEEGVEMMLLANPVRIMSDGCGKVSGMECIRMELGEPDASGRR
                     RPVPVEGSEFILDVDTVIVAVGQGPNPLLTERTKGLELNRKGNIEVRDECGATSIPGV
                     WAGGDIVTGAATVISAMGAGKRAARGIDEWIRSKF"
     gene            52876..54546
                     /locus_tag="PLU15_01950"
     CDS             52876..54546
                     /locus_tag="PLU15_01950"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOO29715.1"
                     /translation="MLSDIEIAQSTKLRPIIDVAKEVGLTEDEIEYYGKYKAKVSLDV
                     FDRLKDRPNGKLIYTTAITATPAGEGKTCMAVGLTQALGKLNKKVMVCLREPSLGPTF
                     GIKGGAAGGGYSQVLPMEDINLHFTGDIHAVSTAHNLLAAMLDNHIHQGNDLNIDIHR
                     VAWRRVMDMNERQLRNIVCGLGGKANGTPRESGFDISVASEIMAVLCLASDMEDLKAR
                     LGRLVVAYTRDGQPVTAADLKAVGAMAVLLKDAIKPNLVQTVEGQPAFVHGGPFANIA
                     HGNNSVIATRMALRLCDYVVTEGGFAADLGAEKFFDIVHRFTGLTPDVAVLVASVRAL
                     NMHGGVPKSKVAETNLDALEKGCANLDKHIENLKKFGLPTVVAINRFPTDTPEELELV
                     KKHCDALGVRSAISDVVARGGDGGIELAQEVLEAVENDQNNFKPIYDPNMPIRDKIEI
                     LATEIYGADGVVYTPSAEKEINRLARLGFDKMPICVAKTQHSLTDDPNLKGAPVGWKL
                     TVRDVRVSAGAGFLVILTGDIMTMPGLPKAPAAENVDIDENGTIVGLF"
     gene            55291..55425
                     /locus_tag="PLU15_01955"
     CDS             55291..55425
                     /locus_tag="PLU15_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29716.1"
                     /translation="MTVGDVIKAAPDPGPFGCGFGSWGMLLILLAIIVLAAFLPRFCW
                     "
     gene            55751..56113
                     /locus_tag="PLU15_01960"
     CDS             55751..56113
                     /locus_tag="PLU15_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOO29717.1"
                     /translation="MKSLDDLKKIKERAQAMTELREGNEEVRIVIGMGTCGIAAGARD
                     TLAAVVDEINTRNLRHVAVTQTGCIGMCEYEPIVDVIRQGEPKVTYGRVTPEKARQII
                     ASHVVNGQVVGDLVISSK"
     gene            56242..56757
                     /gene="nuoE"
                     /locus_tag="PLU15_01965"
     CDS             56242..56757
                     /gene="nuoE"
                     /locus_tag="PLU15_01965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOO29718.1"
                     /translation="MLKMRGPVGPEGPGVVCECDDVFLTEVQRVVSVYKERPEDLVQA
                     LHDVQSLRNYLPREALRVVARTLDVPESRVYGVATFYSMFSTSPRGRHIIRICESAPC
                     HVAGAAEIINSLLNELGVEMGGTTSDGIFTVETSSCLGVCGVAPAIMIDDTVYGNLVS
                     EDLPAVLRRYR"
     gene            56795..58468
                     /gene="nuoF"
                     /locus_tag="PLU15_01970"
     CDS             56795..58468
                     /gene="nuoF"
                     /locus_tag="PLU15_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOO29719.1"
                     /translation="MEFYRAHVLVCGGFPCVAAGCRAVRGEIERSVKEHGLEREVRIV
                     ETGCLGPCDLGPIVVVYPEGTVYSRVSVDDVSEIVGEHLMKGRPVRRLEYTERLPSVS
                     QADHKRPDYFGIQERIVLENVGLIDPDSIEEYIARDGYLGAGMALTSMSPDEVIGAIK
                     ASGLRGRGGAAFPTGVKLGFTARVEADQKYIICNADEGEPGTFKDRLILEGDPHKVIE
                     GMIILGYSVGATMGYIYIRGEYYLSIERIERAIAEARRYGLLGHDLFGSGFSFDIEVK
                     KGAGAYVCGEETALIESMEGKRGEPRQKPPYPGTCGLWGKPTVVNNVETIANVPPIVR
                     NGADWFRQFGTPTSPGTKVYTLTGDVNNKGLIEVPMGITLREVIYGVGGGIPGGRRFK
                     MAQTGGTAGGCLSETHLDIPMDYESLQETGSALGSGALLIIDDSHCVVDIACALLRFF
                     EHESCGQCTPCREGTRRLHDIFRKTRELAATEKDLKLARELAGVMQASSLCALGQSVA
                     VPLFTIMDHYADEIEAHLEGRCPAGVCRPNPANADESAYETLQAGNGIR"
     gene            58488..60218
                     /locus_tag="PLU15_01975"
     CDS             58488..60218
                     /locus_tag="PLU15_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOO29720.1"
                     /translation="MSDITLTIDGKSVCASPEMTILEAARTVGIKIPTLCWHEDLGSP
                     SVCRVCVVEIEGQNTLQPACSYPVSQGMTVRTNTPRVRNARRMAIELLLAHHPDDCLS
                     CQRNLKCELQQLAADFGIREIRFERVSRKIPRDESTPSLVRDADKCINCRRCIEACEK
                     IQGVAVLSTVNRGFDSVVLPAFGDDLDSVVCVLCGQCTLACPTGAIVERDDTGRVWDA
                     LADPNMHVVVQTAPAIRASLGEELGLPAGSIVTGKLVAALRKIGFDRVFDTDFAADLT
                     IMEEGHELLDRIQKGNAPLPLLTSCSPGWINFVERFYPDLLPHVSTCKSPQQMFGAVA
                     KTYYAERADIDPARIFVVSIMPCTAKKYECQRPEMRSSGFQDVDAVLTTRELGRMIRE
                     IGIDFDSLPEEEYDAPMGISTGAGAIFGATGGVMEAALRTVYAVVTGRELSEDGLEFH
                     DVRGLEGIKEAEVDLEGATVRVAIAHGLGNARKVMETIRTGEAPWQFVEIMCCPGGCV
                     GGGGQPIGTVMSSRAERGQALYEVDREMPLRESHKNPAVLALYQEFLDWPLSERSHEL
                     LHTHYTPKKS"
     gene            60422..61762
                     /gene="rsxC"
                     /locus_tag="PLU15_01980"
     CDS             60422..61762
                     /gene="rsxC"
                     /locus_tag="PLU15_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOO29721.1"
                     /translation="MPKGSFRGGVHPPENKERTATLRTVVARRPSQVAIALHQGSNCP
                     LTPTVAKGDYVRMGQKVADTDEKWGVPAHSPVSGKVVSIGLVRTASGVEDQAIFIEPD
                     GEDALDESCVPVDNVFSRSPEEIIRIIRDAGIVGMGGAEFPTHIKLSLPPGANVDTLL
                     LNGAECEPYLTADHRLMVERPRDIIRGAQIMMRAIGVSRTLIGIEDNKPDAIRAMGDA
                     ASSAAGVEVVAVKTRYPQGSEKHLIKSVLGREVPPGGLPFTVGVVVSNVGTCAAVSNR
                     FDRGLPLIERIVTVTGSAVAKPANLVAKIGTSASDLIEECGGFVGQPGKIVFGGPMMG
                     MAVSSVDVPIGKGTSGILVMSREESEDLKEMPCIKCGQCVDACPMRLEPVFLHQWSNA
                     GLWDEAEAYHAMDCIECGVCTYVCPSRRNLVQAIKRAKFEISARRRAAKERGSK"
     gene            61762..62742
                     /locus_tag="PLU15_01985"
     CDS             61762..62742
                     /locus_tag="PLU15_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOO29722.1"
                     /translation="MGNTKLIVTPAPHIRSGDSTPAIMRDVVIALLPAAISAVYFFGA
                     QAAVSMVCSVAGAVGAEYIFEKIAKKPVTIGDWSAAVTGLLIAMVLPPHVPVWIPVIA
                     GFFAIGVVKLLFGGLGHNVFNPALAGRAFAIAAWPALTTSGYLWPASAEGAIWAVRGF
                     DAMSTATPLTLWKMGEIGGASMSKFYQSLFVGNVAGSLGETSALALLIGAVYLLCRGR
                     ISLRIPLSYILTVAVITGLYGQDPLFHVLSGGLILGAFFMATDYVTSPINPKGQIIFG
                     IGCGILTALIRMFGGYPEGVCYSILLMNAAVPLIDRLTRPKRFGEVTSRA"
     gene            62735..63322
                     /locus_tag="PLU15_01990"
     CDS             62735..63322
                     /locus_tag="PLU15_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOO29723.1"
                     /translation="MRDVLKLGLVLMVICAVSGGLLAYVHGVTSEIIDERKAQEVADA
                     MRVVLPAAESFEKLSDDELASIKADPKFVDVDEVYEGAHDGSLVGIVAKVQSPGYGGK
                     ISLLVGMDPDLTVTGLQVLGHSETPGLGANIAKPEFISEFIGRGGSHPLAVDKDGGEI
                     SAISGATISSRGMVNGVNLVRDLVNALGIAGGVGR"
     gene            63319..63945
                     /locus_tag="PLU15_01995"
     CDS             63319..63945
                     /locus_tag="PLU15_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOO29724.1"
                     /translation="MSPLKAFTNGIYKENPIFKLLLGMCPTIAVSLAVSNGIGMGLAA
                     TFVLVGSNLIISLLRKFIPSEIRIPCFIVVIATFVTIVDLSMEAYLPQLHQVLGVFVP
                     LIVVNCIILARAEAFASKNGPVASIMDGLGMGLGFTLALVVISLIREPLGTGVIFAAA
                     DLGFDGIKLFPAEYAASVVTQPVGGFITLGLLLGLVNLITDKKKDKQG"
     gene            63961..64557
                     /gene="rsxA"
                     /locus_tag="PLU15_02000"
     CDS             63961..64557
                     /gene="rsxA"
                     /locus_tag="PLU15_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOO29725.1"
                     /translation="MRQLLVILFSQAFVNNFMLSRILGTCPFIGVSQQVETATGMSLA
                     VVFVMTVASAITWLVQNFILNPLNMAYLQTVAFILVIAALVQLIEMVIKKSSPSLYRA
                     LGIYLPLITTNCAVLGVAIINYQANYNLLESAVSGAAGGLGWALAIILFAAIRERQRL
                     APIPRAMEGFPIALVTTGLLAMAFLGFSGFDVARFMGL"
     gene            64606..65442
                     /locus_tag="PLU15_02005"
     CDS             64606..65442
                     /locus_tag="PLU15_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOO29726.1"
                     /translation="MSSLNALLSMGAMGLIFGISLGAASKKLAVEQDPRIDQIMEVLP
                     GANCGGCGFAGCSALAGAIVSGDAPVTGCPVGKSAVAEKIAKIMGVECATTEPKHARV
                     LCRGSRKNAAELYEYRGLQDCRSAQLTGGGAKVCTYGCLGFGSCVAACQFGAMSMGPD
                     GLPVVDDSLCTGCGKCVGVCPRGLIELRAAGESVDVVCKSHARGPVVRQACSVGCIGC
                     GLCARNCPEKAIEIVNNLAVIDQAKCTRCGICIEKCPQKCIVDSAGVEGITPAKKDEA
                     AV"
     gene            65727..66302
                     /locus_tag="PLU15_02010"
     CDS             65727..66302
                     /locus_tag="PLU15_02010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOO29727.1"
                     /translation="MSRLILASASPRRSQLLHGIGLDFEVIPSNVVEEEVNGADPADL
                     VMRLAYAKAADVAGGIDHGLVIGADTIVVADGHVLGKPVDRDDAIRMLHKLSGKEHQV
                     YSGVSVIDAGSGNSATEYEMTNVKFRTVSDEKIARYVDTGEPMGKAGAYAIQGIGGLL
                     VESIRGDYNCVVGLPLGRLALMLESFGYVVF"
     gene            66355..67074
                     /gene="radC"
                     /locus_tag="PLU15_02015"
     CDS             66355..67074
                     /gene="radC"
                     /locus_tag="PLU15_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOO29728.1"
                     /translation="MKQVDPGRCGMGLSIREMPPSERPMERLLELGPEALSTAELIAV
                     IIRCGRPGESAVDVASRVLSECGTARKLATAGVCELSSIPGIGPVRAAQIVAAIELGR
                     RVVVSEGEPRLSISSAKDVVDLLMPSMRYLEKEEFRTIFLDTRNRVIESATISIGTLN
                     SSIVHPREVFRAAIRAGSAGVILAHNHPSGDPAPSPEDIAITKRLVRAGALIGIEVLD
                     HVIIGDNLYISFKEKGYMNFG"
     gene            67109..68149
                     /locus_tag="PLU15_02020"
     CDS             67109..68149
                     /locus_tag="PLU15_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO29729.1"
                     /translation="MACASLFGGLARDMGIDLGTANTLVYVRSKGIVLQEPSVIAIDR
                     DSQEILAVGYDAKKMVGRTPASIVAVRPMRDGVIADLDVTVTMLRHFIEKASKRHGVF
                     KPRVVIGVPSGVTQVERRVIHDSAIQAGAKEAFLIEEPMAAAIGAGLPVEDATGNMVV
                     DIGGGTTEVAVISLGGIVAAKSITTAGDKMDEAIVNYMKRNYNLMIGERTAEEIKKTV
                     GSAYPSQQEETMEARGRDIVNGLPKTVTISNTEIQQALADPVGEIVNAVRMTLEKTPP
                     ELSADVIDKGIVMTGGGSMLQGLDRLLCDVTGMPVYLTEDPLTCVARGAGNVVEHLTT
                     YRRVLVSSGRLK"
     gene            68171..69022
                     /gene="mreC"
                     /locus_tag="PLU15_02025"
     CDS             68171..69022
                     /gene="mreC"
                     /locus_tag="PLU15_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOO29730.1"
                     /translation="MNQQPLSKRRIVVAAICIILLVCLIYFTAKDRDRLSVAEKALRE
                     IFSPIARGASKVSREISSWASYLISIGETKAENEKLREELDALRYENLQMEEALRENE
                     RLRDLLEFKESLGGRAVAARISYRDPGNWLYSVAIDKGSDHGISSGKPVVTARGVVGR
                     VTSVTRNTSTVILTVDSRSAVGGIDFRSRDLVLVEGLGDGSGLLHVRPLGADNDVQPG
                     DIIITSGLGGIYPKGYVLGDVISVAPGKYGVSKSAFARPRVDFDRLEEVLVLIDPIRG
                     AIGEMQE"
     gene            69019..69534
                     /gene="mreD"
                     /locus_tag="PLU15_02030"
     CDS             69019..69534
                     /gene="mreD"
                     /locus_tag="PLU15_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOO29731.1"
                     /translation="MKRLNRLHIALGMFVALLLQAAVFPVLFPRWVVPDLVFCFSAVM
                     SIIAGTGPGVVLAAIGGLCQDALSGSFMGASAGANMAAAWIIGLVEPQLFKENLITPV
                     VIVGAGTVIREVIYTFVIWSFGAGIDWARAFGTVIPWVAVLNCCAGALLHYWIYLRPR
                     DRERLRSVSSV"
     gene            69568..71370
                     /gene="mrdA"
                     /locus_tag="PLU15_02035"
     CDS             69568..71370
                     /gene="mrdA"
                     /locus_tag="PLU15_02035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOO29732.1"
                     /translation="MMNRTRTQSERRLRVFQIFALCLFLVLVGRLWQLQIIKGEYYKS
                     RATANRLALVPISAPRGIITDRRGEVFATSRMAYTISVVPQEFSDREAEVRLLSKLLE
                     MPVEEIEAKIAGQTEGRYGLPYMPTRLIEDVPPYMLVRVSEHKVELPGVIIEEEPYRS
                     YPNGSLAGPIIGYVGLINQEELARLGEKGYQSRDRIGKSGVEREFEEYLRGQDGVTEI
                     EVDSLSRPVATVGARAPVAGSTLVMTIDAKVQQSAEDALRSQLETLQSGKYKHATAGA
                     AVALDPRTGEIIAMATEPGYDPGWFVPRISDEQWRQVSTGVSGLFNRAISGAYPPGST
                     FKPFTAIAALEAGVIDLDDRILCSPSVSSRYYRKRCSIWSRGRSHGYQNLTEGMINSC
                     NIVFYELGARLSVDQMASAAKQFGLSQPTGLRFTPVEATGAVPDSSVRDFMPGELASY
                     AIGQQVTVTPLQMAVAYGGIATRGVMYKPHLIRQVIAPDGTVQTQSVPEVMRTAVLSD
                     RTWDYLHHSLSEVTRIGTASSAFRNFGIPVAGKTGTAEVPPGDPHAWFVGWAPADDPE
                     IVVAVMIERGGGGGASAAPVARKIMEAYFAPTGE"
     gene            71451..72131
                     /gene="minC"
                     /locus_tag="PLU15_02040"
     CDS             71451..72131
                     /gene="minC"
                     /locus_tag="PLU15_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOO29733.1"
                     /translation="MIFKGTRDGLRLVIDADENIEELKGRLRRRLNEAEGFYSGARVR
                     LDTRGAVVPPEWIEEVRMILEEYGLSLSEEKPGQRRPRQHAAGAGRASAKKVLDDGGP
                     ESGEPIVRGDTILVRRTLRSGQAVSFDGNVVVMGDVNPGSEVLATGDIVVFGSLRGMA
                     HAGSAGNAEACVVALRLIPTQLRIADKITRAPDDETQVPQGPEVAYLREGSIVIEPWT
                     THTGTVLV"
     gene            72131..72982
                     /gene="minD"
                     /locus_tag="PLU15_02045"
     CDS             72131..72982
                     /gene="minD"
                     /locus_tag="PLU15_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOO29734.1"
                     /translation="MSGTAIVVTSGKGGVGKTTAAANIGAGLAMSGPTVALVDADIGL
                     RNLDLVLGLENRIVYDLVNVVEGGCDVQKALIRHKHIENLSLLPAAQSRDKTAVTPEQ
                     MKALTLGLKESFDFVILDCPAGIEQGFKNAIAGADRAIVVTTPEMSASLTADISGVVT
                     PEMSAVRDADRIIGMLEAEEVKDPWLIVNRIRPDMVKRHDMMNVDDLIDFLKVELLGV
                     VPDDESIIISTNRGIPAVMDEKSRAGRAFLDIASRIMGNDVPIAATLEPDDWFSRFAK
                     WLGISRG"
     gene            72988..73269
                     /gene="minE"
                     /locus_tag="PLU15_02050"
     CDS             72988..73269
                     /gene="minE"
                     /locus_tag="PLU15_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOO29735.1"
                     /translation="MMDFLARLFGRVETETSRECAKNRLRLVLVQDRATLSPRVMEQL
                     KSELIEVISKYLDIDEEDMRVELDTSDSSVALVASIPVKEVKRESVGAV"
     gene            73338..74456
                     /gene="rodA"
                     /locus_tag="PLU15_02055"
     CDS             73338..74456
                     /gene="rodA"
                     /locus_tag="PLU15_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOO29736.1"
                     /translation="MLIDRRLLSNLDHALLVAVVIAVLFGCAMIYSATRWNTRLTAGN
                     PLLYVKKQLAAAALGAAASLVVLSVDYHYLERFAKWIYLASLLLLASVFVFGKRTKGA
                     IGWIKIGPVAIQPCEYAKIGVVIALAAYLAQQDDLSDLRSFIVPALIVAAPMALVVAQ
                     NDLGSALVLAPVFFAMMYAAGADAKLLLAIVGVILLVIAPFSYFFLLQTHQQMRIKVF
                     FNPGLDPRGYGYNVMQSVIAIGSGGLLGKGYGQSTQARLNFLPEHHTDFIFSVIAEEL
                     GFFGATAALAVLFFIVHRGYSVAQEARDKFGALLAVGLTSVMLVHIFVNAGMTMNVSP
                     CTGIPLPFFSAGGSALMAMTISIAIVQNVYMRRKKIIF"
     gene            74561..75481
                     /locus_tag="PLU15_02060"
     CDS             74561..75481
                     /locus_tag="PLU15_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOO29737.1"
                     /translation="MKRALPREVPGILGMTRVFGIEFDVDPLVVVFAALYAFAGNLWQ
                     WMVVFASLLVHEAAHAVTAAGFGFAVARISVTPIGAVASLEGDIQLRPEAEAAVAMSG
                     PMTSLVLAAMGYLLLIYGNADRGKIEFFVGANIVLAMFNLIPAFPLDGGRVLRALMTE
                     RLGVASATRKAVGMSRVIGIGIAVLGVAGVAAGYFNFLVPALGLFILIAAERERQSAV
                     CAGLKSVMGKRVSLSKRGALPVRLLVAAPEMTACDLLRKVTSSDFTVVSVIGAGGREI
                     GRLSELELLEGISMLGPDAEVREVLAGRKD"
     gene            75497..77302
                     /locus_tag="PLU15_02065"
     CDS             75497..77302
                     /locus_tag="PLU15_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOO29738.1"
                     /translation="MIDKKVLYEEILPSVSQPARYVGNELNSVRRSPKEVEASILLAF
                     PDVYEVGMSYIGFKILYEIVNNIPEFVAERAFAPWPDMEAQMRLREIPLYGLETYEPA
                     KSFDVIGFTLQYELTYTNVLAMLELAGVPLEADARHEGDPIVIAGGPCASNPEPMAAF
                     FDLLVIGDGEEAIVDLMSEVARAKREGAKRADIVNRLAVIDGVYCPRLKNPVSRRVVT
                     DLDEAEFPRKFVVPFLEAVHDRVALEVMRGCTRGCRFCQAGMIYRPVRERGLDNLCSL
                     AKDLVKSTGYDEISLLSLSSADYTQVEELSRKLVQEYADKGVAVSLPSLRVDSFSLEL
                     AKELEKSRRTGLTFAPEAGTQRMRDVINKGVTEEDLMSAAADAFESGWDLIKLYFMIG
                     LPYEEDEDLDGIADLSRRVLELGRNMAGKRNKAGRVRVNVSVSSFVPKPHTPFQWHAQ
                     NSREELMRKQNYLKERMRVRGLTVSFHNVEASFLEAAFARGGRELAPAIARAAALGCR
                     FDGWSDQFRPDLWAQAFKDVGIDPSAWANKSYAYDEPLPWDHIDMGVSKRFLVREAER
                     AARAELTPDCRTGACAACGACADGVKTRLAGEVWR"
     gene            77310..78029
                     /locus_tag="PLU15_02070"
     CDS             77310..78029
                     /locus_tag="PLU15_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOO29739.1"
                     /translation="MVLRVRITKGEEARFISHLGFMRAFERAIRRAEIPVAYSEGYHP
                     LPKFSFATALGVGISSEAEYVDIQIDPDGPKISVEEAFDALDRNMPPGIAVVAACWYL
                     GKPALASLIEYASYRASGCGALHEAAEEFMAMDASLGTITTKSGEKEEDIRKLVYSIS
                     MTDDESLEFICACGGRAHLRPDNLLVHLSSIAGIECDVKRSRIHRTGLFVVEDGELMS
                     PMDYKCKDLVIAKGWMPRELS"
     gene            78030..79745
                     /locus_tag="PLU15_02075"
                     /pseudo
     CDS             78030..79745
                     /locus_tag="PLU15_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
     assembly_gap    80000..80099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            80737..81051
                     /gene="rplU"
                     /locus_tag="PLU15_02080"
     CDS             80737..81051
                     /gene="rplU"
                     /locus_tag="PLU15_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOO29740.1"
                     /translation="MYAIIETGGKQYRVEPGQVVRVEKLDAEVGDSIEFDRVLMVSGD
                     DGIKVGSPVVDGARVKGAILEHGKAKKIYVFKYKAKKRVRTKTGHRQPFTAVKIESIS
                     VA"
     gene            81089..81436
                     /locus_tag="PLU15_02085"
     CDS             81089..81436
                     /locus_tag="PLU15_02085"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOO29741.1"
                     /translation="MVKLHVRRVEGHVVRFALHGHAECGEYGYDLVCAATSALTIAAA
                     NGLFEHAGVSRAVRYRSGYLDCVILNCPSAEVRMRADAVLETMISGIRAIALEYPESV
                     SVVDIGGSVSARY"
     assembly_gap    81459..81558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            81607..81927
                     /gene="rpmA"
                     /locus_tag="PLU15_02090"
     CDS             81607..81927
                     /gene="rpmA"
                     /locus_tag="PLU15_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOO29742.1"
                     /translation="MIRMDLQLFAKKKGVGSSRNGRDSRSQRLGVKKFGGQYVSAGNI
                     IVRQCGTKVHPGINVGMGKDYTLFALADGYVQFEHNGKRSKKVSVVPELPVAEEPAHV
                     VESM"
     gene            82021..84732
                     /locus_tag="PLU15_02095"
     CDS             82021..84732
                     /locus_tag="PLU15_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOO29743.1"
                     /translation="MGKIPVWARVLIVAIIIVAVGWKTISNITTEYLWYQDLGYVSVF
                     WKCLEARVAVAAAIFAVTFVFVAANLLSMKSASKAMPRRSALFLATIAGVLTAGFSTD
                     LWMEFQQFVHATPFGKADPIFGQDVGFYVFRYPFLKEVYKGAIWVVGLSATFVILVYL
                     FSGSLIRFSREEVEEPAIGSRRRRPRRGRLLLEQADPGTKLHIGILLAIVFCVVAFGY
                     WLDMRGLVYSERGVGIGASYADVHGALPGMRLMLFMSLAAAVIMIISAISQTRVKTLA
                     WMAATVVIMGVFSLLAVNIYPNLVQRLYVTPNELRAEREYISHNIDFTRSAFGLDSIE
                     EKEYVVDQNLSWTDVNSARATLDSIRLWDWEPLLATYGQLQGMRLYYQFNDVDVDRYL
                     IDGNLVQVMLAARELDVESLPPQAQTWVNRRLKYTHGYGVCASPANEIGPDGLPTFLL
                     RDIPPKGSDSLEIERPEIYFGELTRDWVVVNTKTEEFDYPVGDTNKSTRHMGDSGVRI
                     SNPLRRAMFATKFSDARLLLSSDITSDSRILFDREIGQRARKIVPFLSYDSDPYIVVA
                     DGRLIWILDGLTTSDRYPFSERYVGGINYIRNSVKVTIDAYTGQTDFYLIDRNDPVAV
                     TYAKIFPGLFKEADEMPQSIREHVRYPEDLFTIQAKMYGMYHMRDPEVFYNKEDLWAI
                     PNELYGGSEREVSPYYIRLVLPGESDMGFVLFVPFTPSRKDNMVAWLAVNSDMDDFGR
                     MVAYKFGKQKVIFGPMQIEAQIDQDAEISKMLSLWNQSGSTVIRGNLLVYPIGGGLLY
                     VEPLFLAAQTSQLPQLKRVVLCDGAKVAIGSDLWSALDAMLSGHGEGATGSFLDSDEL
                     GNTRELAHQALKLYQQARDRLKDGDLAGYDSIQRELEDLLESMATEE"
     gene            84750..85733
                     /locus_tag="PLU15_02100"
     CDS             84750..85733
                     /locus_tag="PLU15_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOO29744.1"
                     /translation="MVGLAELDLKTNWKDGICEITGFDSFNLHSSCHCGQAFRWRTSG
                     SRGQLEEGVVRGKLLRLEQRDDALIIHPPVDDETVRIAIDYFRLAVPHSPIEEAIAAT
                     DEIMFEAVERNRGLRIVAQEPWECLVSYIISARNSIPQISRTVERIAEAWGAPVFDVP
                     TSHDGGSRDGLVDAAGYEASGKKVWYAFPGPREMCCATVDELAACKTGFRARSVCEAA
                     NRVASGDIDLDGIASLDYPLAKEELIRLRGVGEKVADCVLLFSMGKYEAFPVDVWIER
                     AVRHLYFDGEEISHREIRKWAAGKFGEVAGPAQEYLFAYAREAIPEKLKKG"
BASE COUNT        19560 a        20299 c        25981 g        19583 t
ORIGIN      
        1 ccaccccaaa tggctcgccg ctcaactgcc gttttcctta attggaagca ctagaggtta
       61 tagcacggca gcgataaacc caaacgcaat gagttgcagc atgatcaagg aaaactaata
      121 aaacacagca caattatgtc ctgcataact atacgcttgc attgtatgat gatgcgatat
      181 ataatggtcg gcataaccat tcatcacaca tactgaaagg ggattcgatc taggatgtcg
      241 catcaaacca aaaaagcgtt aactacatgc tttgtatcag tttgtctcgt cctttgcctt
      301 gcatttggcc ttctagtgcc tatgccgacc atggctgcag atgagcaagg cggagatgtt
      361 atggcaacgg cgatcaaatt cgcggaagac catgctgatc ttactgagga aatcgccagt
      421 gcactgtggg ggtacgccga gatagggctt gcggagtaca agtcgtatgt caaggcccga
      481 agtgttctgg ctgaagccgg atttgagatc atcgattcag ctgcggatat cccgacgtgc
      541 ctagttgccc aatggggctc aggcaatcct gtaatcggta tctatgagga tattgacgct
      601 ctgccaggcg ttggccatgg atgcggacac aacctgaaca ccgcagccgg tatcgtggcg
      661 gctatggcta tgaagcatgc catggagaca cacgacatac agggcacgat aaaggtgttt
      721 atcaaccccg cagaggaagt atgggatgtt gctcccctcg ttgccgctgc aggatactat
      781 gatgacgtcg acgcgttgat cagttttcac gcaggaacag aaaatacgtc tgagtacgga
      841 agtacgatgg ctatggacca tgtggaatac aaattcaagg ggcagcctgc tcatgctgct
      901 gctgctcctg agaaaggcat cagcgctctc gatgccgtgg agataatgaa cgtggcagtc
      961 aattacctca gagaacacct gattcaggag atgaggatac attacgtcat aacagatggt
     1021 ggcgccgctc ccaatatagt gcctgccact gccgccagcc gctacttcat ccgtgggccc
     1081 gaatatcctg acgtggctta cgctcggcag cgcatagacg attgcgcgaa agcggccgct
     1141 cttgccacag gcgcccagct cgagatagga tttagttcag gcatatacaa caaggttccc
     1201 aacaagacct tagccctcaa ggccatagat gcgatagact tcgtagggac gcctaagttc
     1261 aatgaggacg acatagctaa gatggcagcc ctcggcatcg ccggcgtgcc atccggcgct
     1321 atcacggagc ctacaggaag tcagagtttc ggttctgatc caataggcga cgtaacttgg
     1381 aagactcctg ccactaccat agggattgct acgtgggttc cgggaactgc aggccactcg
     1441 gtggaggcgg ctctgcagag tggatcggtt tatggtttca agggagcagc agctgcatcc
     1501 aaagcccttg cagtcctggg actggaactg atgacgaacc cggattcact ggctgccatc
     1561 gttgcggaat acgaggatag gatgaaggac atgcccccat acgagggcaa ggcaatgata
     1621 ccggaagtag cttatccgga ggcgcctgga gttgtggtat cagcttcaaa gggctgtgta
     1681 ctgctgacat ctgctgaaac agcgttcgaa gaagccgaag gtgacgtcat agcagtcaag
     1741 tcggcttccg gagaagtgtt tggcgagttt ctcgttgggg ccaaacccct ggacgcagca
     1801 gccgagtatt cctttgctct cagcggaaag atcgagcctg gccagaggct gaagatcttt
     1861 tatactgcag ccggaaccaa cagcacatgg ttctacggct atgttcacgc agaataagct
     1921 gacacaggcc taagacgatg gtcgtcgcct acaccaaaaa ggcggcagct gactggctcc
     1981 caagttgttc agctgccgcc ttatcaacag actgcgagtg cgtatgggtt aaaaaccacc
     2041 gcacacgcct atacgcagtc tacatgtgcg ggcagttttt gctatgcttt ccctgactaa
     2101 gattgaactt gaaaaacggc tgcccccata cctggcaggc caaccacatg ccggccacaa
     2161 ttctcgccta ccgccgacat cgatgcctgc gtattgtcgg atctgccttg accagtattg
     2221 tcgccgtgca ggctgaagat ttgcttcatc ggcatgccgg cccactgctc tagttcgtgt
     2281 gtctttctca tgcgctctgc gacaagcttc gtatttaccg tctgaggctc ttctgtgttg
     2341 ttgagcgcga cgacgatgca cgcgtcatca tcccatctag catagacgta aggactgtcc
     2401 ggcccgtctg caatgagtga tgcgaagtct ccacgcctga ggaccggatg ttcggcccgg
     2461 agccgggcta gggcccgatg atgctcgagg agatcgaggt cccataagtc ttcctcccaa
     2521 ggcatgcatt ttctgcagtc cggatcctct ccgcctttga gcccgatttc atccccatag
     2581 tagatgtagg gagttcctgt atacgtgaac tggaacgtgg tagccagcat cagcctcgca
     2641 gtatctcccc ggcatgcggt gaggaaccgc tgtcgatcgt gggaccccag aaggttccac
     2701 atggcatact gtgtttgtct gcggtagtgc atggctgtgg cggttatgcc gtctgcaaaa
     2761 tcggcagtcc cgattcgtcg gcgggcgaag taatcgagac acagctctct ccaggggtag
     2821 ttcatgaccg agtccatctg gtccccgcgg agaaatggcg aggcatcatg gaggatttcg
     2881 cccactatta ggatgtcggg ctttatttgc cgcatccgcg tcctgaactc gcgccagaac
     2941 ccctggtcta catcgtccgc cacatcaagc cgccaaccgt cgatgtcgag tgtgcgcgtc
     3001 cagtgctcag ccgctgagat gaggtaatcg cggacttcgg gattgtcggt ccggaatttc
     3061 ggcatctgcc atatcccggg accaaatgtt tcgtagttcg gctttggacg tgtgcgcacc
     3121 ggatagccgt ggatattgaa ccacttccag tatggcgagt cttcgccttt tttcaccacg
     3181 tcgcgaaatg cgaaaaagtc gtagccggag tggttgaaga cagcatcaag aacgacgcgg
     3241 attccccggg cgtggcactg atccacaaga tgtcgcgccg tatcctcgtt tccgaaatgc
     3301 ggatcgatgt gataatagtc aatggtgtcg tacttgtgat tcgacgacga aaggaagata
     3361 ggggtgaggt agaggcacga cacccccaaa gaggatatgt agtcgagctt ttcgatgatt
     3421 ccagccaggt ctcccccgga aaaggagcgc ggtgtgggca gcgttcccca tctggatact
     3481 ccgggcgggt catttgcccg gtcgccattg gcgaatctat ccacaaagat ctgatacacc
     3541 actgcgtcct gagcccactt cgggggatca tccatgcctc catgatacca gatgcacggg
     3601 tattggaaac accatgtcca tggctcgtcc tgtggcggtt cgtccgaaaa accgccttca
     3661 ccgtagcaga tgcgtttttc atcatcttga agctcgaaat agtacatgaa gcgcccatat
     3721 tgaagcttca tgcttcctct ccagtaatcg aagagtccat cagatcctgc gatccagaga
     3781 cgagtactgc tagtgcgggc atgtggcgtg tatctgtcgg tgtatactgc ctcaactcgc
     3841 cgcagatcgc ctctggctgc acggagcacg atttcaagcc ggtcatcact gattggatat
     3901 gccattgcat cgtaaggcac atgccatatt gcgtgtttct gcaaccttta gtatccctcg
     3961 tttcttggtg tttgcaattg attattcctt gaccgcgccg cgcgtcagcc ccgataccag
     4021 ctcgttttgg agaagcgcaa agagcgtaag gattggagca gaacctatga ttgcggcagc
     4081 ggaaaacacc ccccaccttt ttgcatactg atcgccaatg aacgattgga gtcctactgc
     4141 cagcgtaagg acgtcgtttg acgacaacag gacctttgcc aggacatact ctgaataggt
     4201 cccaatgaac tggaggaaga agataaccac aagaataggg cgggacagcg gcagtattac
     4261 cttgtagaaa cattggaacc gtgttgcccc gtctattgtc gccgcttcct ccagcgacgt
     4321 gggtatggtg tcgaagtagc ctttcatgag ccacacgtta acccccatgg cgccgcccaa
     4381 atacaccaga atgagccctc catgggtatt gaggccgaac gtcggaaaat gctcgccgat
     4441 ccagagcagg agtaagtaga tagcgaccat cgccagcatc tgcggaaaca tctgaatcag
     4501 caggatcgag aagagcccgg tctttctccc gcggaaacga aatcgggaga aggaataggc
     4561 ggccaacgac gtcatgatga ccaccagggc cgacgtgata aatgagacct ttaaagtgtt
     4621 tagcatccac cgtccgaacg ggtgagtggg gtcattaaat agctttctat agttctccag
     4681 tgatggattg ctcggtataa gcttctgccc gattatggtg tttgaagggt cgaacgaagc
     4741 cgatattatc caaagcaccg gcagaatggc gaatgctatg cacagccata tgactatgtg
     4801 gcgccacaag taggccggtt ctaagaaccc cttatacatt ctcgtgcacc tcctcgaggg
     4861 cgcctgtgaa gcggaagttg aaccagctca gtgtgccgat tattaggaag atcaatatgg
     4921 ttactgctga agcaagcccg tagtcggttc cagaaccgct ttcaaaagcc agacggtaag
     4981 tgtaagtaat cagtatgtcg gttgcccctg caggagtctg cgcgccggga atagccggtc
     5041 ttccctgggt gaccaggtat atcacaccaa aattgttgaa attgaaagcg aaactcgaca
     5101 caagcaaggg cgctacgctt acgagcagga gcgggaatgt gacatgccag aactgctgcc
     5161 agccggtggc gccgtctacc cgggaagcct catagagctc gtccgggatg ctctgaagtg
     5221 aaccgaggct gactagcatc atgtacggga atccaagcca aaggttgact attatcagtg
     5281 cgaccttggc ccagaatgga tcctgcagcc acggcactct cacgttgaaa aggaactgga
     5341 gcatgtcatt gatgagcccc acttccgcgt tgaggaggcc tctccagatg agcgcggata
     5401 tgaaggcggg catcgcatag gggataacca gcagaactct gtagaatcct ttgaacttca
     5461 ttttctggtc attgagaagg acagcgagga gaaggccgag gataaacgtg gttaccacag
     5521 aaagcgccgc ccattcaaaa gtccatacga acaccctgaa aaacgggcct gacacctggg
     5581 gacttcggat taacctcgca aaattgctcc acccgaccgg cgcacgatag cctacgtcca
     5641 aagcttcccc gtcgtctgcc agaaaagtgc cttgcgtcgc tgtataaact tttccggtgg
     5701 tcgtgtcaat gagggcatcc agattgctat cgtaggaata ttgaggctta tatgtggcaa
     5761 accgatccac gttcctcatc ttgagtataa agtcctcgaa gtggaactgg agagcctcta
     5821 ctgcactcat gtgctggaag agttcgagcg tgccgaggcg ctgatagggg cctatgcttt
     5881 ccggaacgcc atctgaatcc gcgtattcaa cgggcagttg gacctcgcca agcgggagaa
     5941 cttcaccgtc tacgccgagc aacttctctc ccttagggtc gatgaggacg actgcgaatc
     6001 tgccgtcatg gcttttgtac attgcagcag ggtactccgg cgcatcttcc tgcagggtgg
     6061 cccgggagag gtagttctcg attacctgtt cttttgtgag gaggtggccg gtgccgtagt
     6121 ttgtgaaggc cacagccacc gtgtaacata tgggatacac caccatggct gccatgaaga
     6181 tgaggcctgg aatcaagtag cgccatggga acaggcgcgg agtcaggaaa acccaatcga
     6241 tcagagcgca tcccgctgca agcatgacag ctatccaata ggcgctttcg cctatcagaa
     6301 cgaatatggc ccagatggcc aatgcgttga gtattcccag gagtgcgacc tttgcaatca
     6361 atcctggaat cggaatatcg acgggcgcgc gtcttgtcgc gactttcaag tcatttcacc
     6421 ctccctgagc tagcgcaagg gcgcgcgccc ttgtcgaact gtcggcatga cgggcggcgc
     6481 ccccacgaaa tcaggcatcg cgtgaacatt gtgcaaacct atttcttgct agccttgatg
     6541 gcagagatga tctgtgccgt tgcgtcatca agagctttct tcggttcgag cttctggttg
     6601 aacgtgagtt ctatggcgtt ggcccatgcc gtccacactg cggacatttc cggtattgcc
     6661 ggcataggta tgccagcggc tgcgctatcg cccgtagccc ttatgtctgc gtcagacgac
     6721 acggctcttg ccgtcgcaag atacgccggc ggacggggat cctgcttgaa cagggccatc
     6781 atagtgtcgg cagtagccac gtattcgttg aggaaggctt tggccagcat cttgttcttg
     6841 ctgaacgagc tgatcatgaa gccttgaact ccgacaaacg ggcggggatc ctttccgtct
     6901 atctttggta tggtggtgaa cccgtaaggg acctttgctg cgcgaaggtc ggccagggcc
     6961 catggccctg tgagcatcat gcctactttg ccatctttga agaggcccat cattgtgttg
     7021 taatccatac tttgcggcat cagtccgtcc tcaacaagct tgtcgaggag gctgaggcct
     7081 ctgactgcgc ccgcattatt gagccccacg tcgagcgggt tgagggtgcc atccttgttc
     7141 accccaaata cgtatccgcc ggcggcgctc atgatcggga atgtgtggta tgggtcgggt
     7201 atgggaagaa ggaatccgta ttgcttctta tctttgtcgg taagcttttt gcagattgcc
     7261 accaagtcgt cccaggtctt tggcggattg ggaacgagct ttgtgttgta gaagagcccc
     7321 actgcctcca cagcgtaagg aactccgtac aatgtactgc cccatgtgaa ggcttcaacc
     7381 gagactttga ggaaatcttt ctctttggcc tgcaggtcga tgggctcgat aaggccgttg
     7441 gttacgagct ctcccagcca gtcatgggca cctatgatga tatcggggcc tttgcctgcc
     7501 ggtccggcca cgccaagctg gtcgcggatg tcgccaaagc ccagttcttg aacttccaca
     7561 ggcactttgt acttggctgt aaacttttta cctaggtcct ccatgacagg ggctcgcgtt
     7621 ccatctgccc atatgagaag tttgccttct tgagatgccg acgcgcacgt agccaatcct
     7681 agcagcattg agatgcacat ggcaattgca agagatcttt tcatcttcgc ggctgcgcac
     7741 tccaagcact cgttgcgcct gaagaggcgc ctttcccccc tccattttta atgacaagca
     7801 tgctccccgt gatgctgact gggtcgtatg tttatgccat ggatgctgcc tatctatacc
     7861 tgtagtgcag tgtttgggga acacttgata aattctccgg aagaatcaca aatcctgcat
     7921 tgataaaaca ggatgggatt tgggctattg ccctgggagg ttagcgtatt gcccatgggc
     7981 cgatgggtgc tgcccggtat ttacgaacct atcggaccct ggcacatgtg ctttgggtcc
     8041 atggtcaaac ccatgtccgt gcgcatatgg tgcactagac tctaaccagg agagtgatgg
     8101 gcaatgacag atgaagaaag ggaagaggtc gaaacgcgcc aggcgccggc ttgtccgggc
     8161 ggatttctat atgagatcag ggctggcgac acattctacg ctctggcccg acgatacggg
     8221 acaactgtag aggctataac ggcagctaat cccggcgtag acccgcgaag gctgcagatc
     8281 gggcagctta tctgtatacc cgtagctgtc ccgacgccgc cgacgccccc cgtgcctccg
     8341 gttctctgtc ctggtgggac gccttatgtc atacggcaag gcgatactct atacaggata
     8401 gctcagaggt atgggaccac cgttgatgcg ttgctccgcg caaatccagg aatcgatccc
     8461 atgaacctcc agattgggca ggttatatgt attcccgtgc cgattcccgt tcctcctccg
     8521 acggtttgtc cgggaggagt cttctacact atcagagcag gagacacgct atacacgatc
     8581 gcagctgcgt acggaacgac agtccaggca atactggcgg ccaaccccgg cctcgatccg
     8641 atgaggctcc aggtaggcca ggtaatatgc gtgcctctgc ctgctgtggc gcccagaacg
     8701 tgtacgctcg ccctcgctcc gtctgtgaca ggccctgtaa caacggcagg aggcgccctg
     8761 tggatgagaa ctgatgcatc aggtaggact gagcttctgg ttatcatcac taatgtgccg
     8821 tcacctacaa gccttggagc ggctacttac acggccctgt ttacttgggg tgggtattca
     8881 ttccaggtgc ctatgacaga ggcgctgggc accgggatct gggctggcac tgcagttcag
     8941 cagttgcctg cggccttttt ctcaaccggc agcgtaaacg tgtggccagg tccagtcctt
     9001 ggcggactcg tggcaaactg caagtaagag tcgggcgcgt gggtaacgat gccgacagag
     9061 ggtcggaaag cttgccattt aaggcaggct ttccggcatt tacgcggcat gtttgttggg
     9121 cctatggaat gaaaaaagtc caagcagctt tgaaaaagta gttgagcgcg ttcaaacggc
     9181 tatcgcatgg gcaccgcctg gggaaagttg cccctgtagg aatgtgtata tagggcaaag
     9241 cctagttcga gattgctttg agcaccatag aaggtacgta cggttcggtg aggagatggc
     9301 gccgggctga tcgcatggcg ccaccatatt aaatgtcgtg gcgacaatgc tccgcgagat
     9361 tactcacaca atcaggcagg tgcacagcag cgatgtcgcc cataatgaac aggttgttgt
     9421 gtggttgatc tacgacttcg gcagcacagc tcatgccagg tgggaaatgc aatttacgga
     9481 ttccacaaca taacctgcct cggtgtaatc caccacgttt atgcaacatg gatcctgaga
     9541 cagcttccaa aaactcgata caggggcatg caacatattg agcaaaatga gcttagtcag
     9601 cacgcggtgg gagacgattg ccaactcatc gtcatgctcg tgtgatgaga cgattttgtc
     9661 cattgcagcc atggcacggt cagatacgtc tgacagagac tcgccaccgg ggaaggtaaa
     9721 tgaatcgggg cctgtttgcc agtccagata gagtcttccg tagtctgacc ttatccggtc
     9781 ggtggaaagc ccttcccatt ccccgacatc tatgtctgtg agcgcgtcgc gaacgatgat
     9841 gtttatgcca tgtgagacag ctatcgccct tgcagtgtcg acggaacgcg acagagggct
     9901 cgtatagacc gcaacgagtt tgacatcgcg aagcgccgta gcgaccgccg cagcttgttt
     9961 tctgcccatg tcatcgaggg gcgcatccat gcgtccacgg aaggtctctt cgcgcccgcg
    10021 gacggtttgc ccgtgacgaa caagatatat tctcatccga ttaacacctt ctcttctggg
    10081 agatggacat tggccaaggg cttttgccgt tgagccaaag ctaacatcac agtgaggggg
    10141 ccgagacgcc caaaaaacat ggttatcatt ataagcactc tgccgaagtc agtaaggtta
    10201 ggcgttatgc ccgttgtcag accgactgtg ccgaaggccg acgcggcttc gaacaacacc
    10261 tgaaggaacg aagcctcttc tgttgcggag agtaccaaag tgacgcctgt taccaaggcc
    10321 agtgcgagac tggcgatggt gatggccctg aacaccaggc tttgggatat ctgcctgtcg
    10381 ttgatctcca catcgtctga accacgaata gtggagagta tggctgcgat gatcactccc
    10441 attgttgtgg tttttatgcc tccgccggtg gaactgggag aggctcctat aaacatgaga
    10501 attacgaaaa tgaacaaggt cgagcttctc atcttgctga tgtcaagggt gttaaagcca
    10561 gcggtccgcg gggttatgcc ttgaaagact gcgccaagga ctttgcctgc cggagacatt
    10621 tttccgagtg tgtcggggtt gctgaactcc aacccgaatg tggcggccgt gccgatggcg
    10681 agcagccaga gtgtgactgt caagaccagt ctggcatgga gcgacagcct gttgccccgt
    10741 cggtgaccaa caagttcgac tatgactgga aagcccaggc ctccaatgat tatcaacgat
    10801 cctatgacga gaacgactgt gggatctgat gcccagtatg tgaggctgga gaaaggcccg
    10861 gttatagagc caaaaagatc aaaacctgca ttgcagaacg ccgatattga atggaatatt
    10921 ccgtagtata ttgcctttga tgtgggcatg atcgacgaaa acctaaggct cagaatgatc
    10981 gccccgacac cctccacgat gaatgtggtc aagaggaggt atctggtgag gcgcaccagc
    11041 cctgcaatgc taaactggtt gagggattct tgcagaagaa tcctcccccg cagcgtaatc
    11101 tttcggccca aaaacaaggc gattgctgtg gccagcgcca tgaatcccaa tccgcctatt
    11161 tgtatcagga tgatgattac tatctgccca aaaagcgaaa aagatgtggc tgtatcgagc
    11221 accacgaggc ctgtaacgca agtggcggat gtagcggtga aaatggcgtc aataaaccgg
    11281 atgctttttc cgttggctga tgctgcagga agcgaaagca gaattgcccc gatgattatc
    11341 acggcagcga aacccgtgac cagaacctgt gatggattga gtttagcacg atgaaacctg
    11401 tggaagaatc cgttgagcgc atccatgttt tcagtcatac ccgctttcac tctcctgaat
    11461 gcaaacaatc ctatcatcgg ggtgttcgcg tgtcaattgt atcaaattga cggccgtttt
    11521 accctcgcga actgcgtaac caggtgcaag ctggacgcag aatcggaccc aatccagctc
    11581 atggcttggt gcacttagtg ggtgtggcac gtagagagtg attttatggt gttgaaacaa
    11641 gattgagaag agtataaaat atgtcatact acttatcgac actgagaggt gatgatgggt
    11701 ggacgaccca agtagcagta cctacttgtt tgcctttatc tgcttggtgt tgtctggagc
    11761 tttttccgcg agcgaggctg cgattctctc atccgatgag gcaaggctca agagtcgata
    11821 ttcatccgac ccccgactcc gttcgattct tgctgtgaat aagagccctg ctcctgtcat
    11881 gtcgtctatt cttcttggca atactctggt aaacgcaatg ttctctgcac ttgcggcatt
    11941 cataattcac tctattgtac cgggcgatgc tgggctcgct gacgcaattg cgacggcagc
    12001 ggctacggtg atgcttctcg tattcgcaga ggtaagccct aaattggtaa gtggaggcaa
    12061 cccagaagcg gttgcacttt cggcttcatc ctggctgtta ggtttggctc gtgcgctggc
    12121 gccggtatct agcgtgctga cagcattgtc ggggcatttg gtggcatgcc ttccccaacc
    12181 ggacactgcg gaggaggatt tcggggaact gcgatttctg gctgcagttg aatacggtga
    12241 agaggctggc gccatatccg gtctggaaaa ggagatgata tggggggcca tggaaaccca
    12301 tgacctcttg gccaaagaca taatgactcc gaggccgcgt attgtggcag cgagtgagga
    12361 tcagagcacc gcagaggcac ttgacttaat gctgaaatac ggtttttccc gcattccggt
    12421 gtacgcggat tccatcgata atgtgactgg aatactcaac atgaaggata ttgctctgct
    12481 cataagctgc tcccccaatg actggcgtga gagattggac ctcatgccat gcgtgcagct
    12541 ggcagtgaga ccctacttta cccatgaaag aaagagagtc tccgatttgc tgatcgaaat
    12601 gcgtctgaag ggcatacata cggcgattgt tgtggacggc catgatggcg tatctggcct
    12661 ggtcacgctc gaagacgctc tcgaggttat agttggggac attcaagatg aatatgacga
    12721 ggagactccg gaactggtga tgtcggaagc agggtgttgc catgtcgttg gaagtgttag
    12781 gctggctgat cttgcagagg cctttgatgt ggagttgcct acggaggaat ttgattccgt
    12841 agccgacttc attatggcat gcctcgacag ggtgccttcc gagggggacg cctactctct
    12901 tgtagggcca agattgactt tggaggttgt ggagatggac ggcccgcgta taaaaagggc
    12961 catagtgcgt ctatcggaga tggagacggg acgccatggg tgagttggac ttattggttt
    13021 ctttgatgat ccggactatt ctgtttgttg gttcagtgtt tggtgtgggc ttctttgcgg
    13081 cgtctgagag cgcgttcttg agcatgaata agtgggcaat tgacaagctt gcggaggaag
    13141 gcgacaagaa cgcgttgatc atgcgcaggc ttacagagga ttccgccaag acagtatcag
    13201 ctgtactggt cggaacgaat gtcctgtcaa cactctcatc tgtcctggcg gtttccattc
    13261 tgttgcttgt agggaaatca gggacgaggg cgatagctat tgcatcgata gtgacaactg
    13321 cgattatact gattttctct gagctgactc cgaagacata tgcggcagca aatcccacag
    13381 ccactgccct gaaggtcgct gttccgttat cctgggcaat tactctgctc ggccctgcgg
    13441 catcattcct tgcttgggtt ccctcgcaga tcgctgcctt gtttaggcga aaatcgaatg
    13501 ctggcaatga gtctcctgat gagatcattc gagcggctct tgatgctgct gaggacgacg
    13561 ggtcagttgt tccgactgag accgaggtga tccatggagt tttggattcc aaaaggaagt
    13621 ttgttcgcga catcatgatc ccaatcgatc ggactgtcgc tttggattac gattccactt
    13681 tggaggattt catggaggcc tacctgaaag aacgctacag ccggatgccg ataatcgctt
    13741 cattctgcgg taaagtgctt ggcattgcac atgtgaagga tgttatgaag gagattgtag
    13801 cgggcggcga tccggctgca aagaaggttc agtcggtggc aaacaccgcc tgtttggtgt
    13861 ctgccgatgc tccggcactc gacgctttca acgagatacg gagccgcaag gctcatctgg
    13921 ccgtagtggt ccagcgcgga atcgctgtgg gcattgtgac catagaggat ttcctcgaag
    13981 agatagtggg cgatattccg gaagatgcat ccggccatgt cgtgaagtcg acctatggca
    14041 caatggccct ggctgtgaca gtagaaggcg ctgagaacgc ctaaagctag ccagaactat
    14101 cgcacaaatc agttttggaa gcgtccatgc aaagatgtaa gcgtgctgtg aatacggtga
    14161 tcaagacctg gtgtccctta tcccgagatg accggacttt cattgccaga ctgattgttg
    14221 ctgaagcctt gtgtgggcta tgcaaggaac taggatgtga cacggggata tgcgtgtttt
    14281 cgaagaaaga agccgagcat tcgatagcaa acgttccatc tgaactgtca gacattgggc
    14341 ctatgtatgc ggccttcgtg ctgttctccg aagccttcta tttcggtgag aacggtcttg
    14401 ccgtactttt cccagcgctc agacgtcccg aatggctgcg ttctcggtat atattctttc
    14461 gatcgcggat ccctgagaga gcgcttctcg aaactgatcg aatagctatg gaatacacat
    14521 tgtatgcaag cggagacagg ctaaacgttg aggcgccagg gggactgata tagatgcgac
    14581 tttgcctggc gccaactgtt ttggaagaga cttcagccaa cggcgttata gtagctgatg
    14641 tgacaattgg ggagctcgtg tacgacggca aactgttgga tgccttggaa gagctagatg
    14701 cgcctgttcg atttatagtg gaagagggca tgtttgccga acgcctcagg aacctgctat
    14761 ggacattacc ccgatccagt cgacacgtgg ttgagctggg aataggactg tcaaatatca
    14821 ctccaacggg ttacatcgga gctgacgagt gcattgcagg cacatgccat tttggaatcg
    14881 gcgacaatag gttttatgga ggaaggaatg cctcgcccat acacctagat gtagttgtgg
    14941 atcggccccg tatcaagcgc tattgactgt gttgtgtttg agacttggca agccgcttac
    15001 agtctggagc aaagctgaag gctttttggg agtcacttgt tgcctcataa gaagtgacat
    15061 tgaacacgga agttcgctct agcgaagcaa ttgtggtcat aggactgtgc ttggcacaga
    15121 attgtgtttg gcaaaacgcg taacaaagtc gtgcgggacc cttgaccatg gggtacgcct
    15181 atgataaaat tcttaagcaa cgtggtggat gtagctcagt tggctagagc atcagattgt
    15241 ggctctgagg gtcgtgggtt caagtcccat catccacccc atcaattcga ttgtgaggcc
    15301 ctgggatatc cagggtctat tgctttacat gcgctttggg ttatgctata ctttgtcgcg
    15361 ggcatgaagc gggcgcccgt agctcagttg gacagagcga tggacttcga atccataggc
    15421 cgcaggttcg agccctgccg ggcgcgccat ttttgtttgc cagagtgtaa ccggtgcata
    15481 tcggggattg gcattgatgc cccggtggtg gcttgaacag ccgctcgccg gggcatacta
    15541 ttgtgaatta aagttttcaa aggaatcaac ttacgcatcg tcgaataggt tacccgaagg
    15601 atgctcggga cagcgaggtg tactcatgta taaagccagt gtagtcgata atgatcaagg
    15661 attagtgact ctggagataa ccgtaagcga agacgaggtt cgtcgcgcgc ttggaagcgc
    15721 ggtccgcaga ctggggcaac gatatacggt cccgggtttc cggaagggta aagcgccccg
    15781 cctaatattg gagaactacg ttggcagaga gatcatatac gatgaagcta tccgtgattt
    15841 cgtgccgaaa gcctatgagg ctgctctcga ggagacaaat ctgattcctc tggaggatcc
    15901 ggcatttgag ggccttgacg atgctgacct cgattcaggc gagccgttaa cgttcaaggc
    15961 gagaatgacg gtgaagcccg aggtaaagct gggagattac aagtctatta agctcgaccg
    16021 ggagaagcgc gaggtgactg aagccgatat cgaccgcgtg cttgagaatc tgcgtgatca
    16081 gcgaggcgaa tttgtttcta cagagcgtac cacagctcaa gctggggacc ttgtgaccgt
    16141 ctcaatcgaa gccatggttg atggggagcg tttggaagag atctcagttg atgacacgca
    16201 agttcccgtg ggtatgggtt acctcatgga aggcatagat gaagagatcg agggcatgac
    16261 ggttggcgaa cccaaatctg tcactgctgt tctccctgaa ggtttttata tcgaggaact
    16321 cgcaggccat gatgttacgt tcaacataat agtaaaagac atcaaggtca agcaactgcc
    16381 tgagttggat gacgagtttg ccaaggattt tggagagacg aaaacccttg aagagctgcg
    16441 caatgatatc aagcattcca tgatgcacca tttccttgat gaggccaaga aggatttggg
    16501 tgaacgcgcg ctcgacgcgt tggtgacgac ctcagaagtc gaacctccca agctcctggt
    16561 agagcaagag gcctatatcg catttgacaa gctgcaggag acagtggaaa aggaggggct
    16621 cgaatgggag gaatacctgg aggcaaaggg tcaatcggaa gatgacgtgt atcaggagat
    16681 gctcgagttg gcgccggcct ctgtcaagaa agcattggta attgaggctt tggccaaaga
    16741 ggaaaacctt ttccctacta ctgacgaagt gagcattgcc gttgctcagg taatgagtta
    16801 tgcgggtgct caaagccgtg agatattgca gaaggcgata gaagatcccg aaatgcgtag
    16861 ggttacgact aaagcactga tgcgtgaaaa agtcttggca tatttagcca atacatgcga
    16921 cgcggatccg gagtctgcta tatgccagga atgtgctgca cgcgaagcgg aagaagcgaa
    16981 ggaaaacgct gagaaggctc aggccgagtc tgggaccgag tcgcgtgacg agggcataga
    17041 gcagaccagt gaagctgatg agccgggttc agatgaggaa atggacggag aagccgtagc
    17101 ggaagaaacg tcgtcaccag agccataggc gcaaatcggg aaatagccga tcagcgcttc
    17161 aggaccgctt ccgatgcggg cgatatggcc aagtcttcga aggcagttgc acgcatagag
    17221 ttacatggag tattattcgc ggcctaaagc agcagaataa tctggactct gctggatggc
    17281 tcagcacacg ttcctggcaa gagttgcata ccccaccttt gcctctcgct gaggggcgtg
    17341 gattgaaacc agagaggtga tggacatgac cctcatacct atggttgttg aacagactag
    17401 ccgtggtgag cgagcatatg atatttactc caggcttctg aaagacagga tcatcttcgt
    17461 tgggggcccg atagatgaca gtatggcgaa tcttgtcata gcgcagatgc ttttcctgca
    17521 gggcgaagat cctgagaagg atatcaatct ttacatcaat agcccaggcg gagtagtcac
    17581 tgctgggctg gcgatatatg atacaatgca gtacgtgaag cctgatgttt ccacggtatg
    17641 cgtcgggctt gcggcgagcg cggcagccgt actccttgct gcgggcgcga agggcaagcg
    17701 ttacgcactg ccctattcca ggataatggt tcatcagcct catggaggca cacagggtca
    17761 ggccacagac atagagatac aggccaagga gatcctgcga attaaaggaa ttaccaacga
    17821 gattctctcg cgccacactg ggcagccaat cgaacggatc gaccgcgaca ccgatcgcga
    17881 cttttacatg tcagcccagg aagctaagga atatggaatc attgacagca ttttcgaggg
    17941 caaggctgca gaggcttagc cgaggggtgg tatcatgttc aagtacggtg atgataaggg
    18001 tcagctaagg tgctcctttt gtggcaaggc ccaggagcag gtaagaaggc tcatcgctgg
    18061 tcctggcgtt tacatctgcg atgagtgcat tgagctgtgc aatgagatca tcgaggaaga
    18121 gtcgggaatc agtcctgaca tcgatttcag cgagatccca aagccgcacg aaatacgcgc
    18181 cattcttgat gaatacgtga tagggcaaga tgacgctaag aagacgctct cggttgccgt
    18241 gtacaaccat tataagagga tcagcgctgc accccatgtc gacgacgtgg agctgcagaa
    18301 aagcaacatc ttgctgcttg gccccacggg ctgcggcaaa acattgctcg cgcaaaccct
    18361 tgcgaagata ctcaacgtgc cttttgccat atcagatgcc acttctctta cggaggctgg
    18421 ctatgtgggc gaggatgtcg agaacatttt gctcaagctg atccagaacg ccgactacga
    18481 tatcgagcgc gcggaaaagg gaattgttta cattgacgag gtagataaga tagctagaaa
    18541 gtcggagaac ccgtccataa caagggatgt gtcgggtgaa ggggtccagc aggccctcct
    18601 caaaatactt gaggggacag tggcgagcgt tccccctcaa gggggtcgaa agcacccaca
    18661 tcaggagttt atccagcttg acacgacgaa tatcctcttc atatgcggcg gcgcatttga
    18721 cggtctcgat agcataatcg agaaccgcat cggtcgaaag actttgggat ttggcgctga
    18781 ggtaaagcca aaggtcgaca agaacatcgg cgatatcctc cgtcaagtta tgcccgagga
    18841 tcttctgaag tatggtctga tccctgagtt catcggcagg cttcccgtgg tcgttcccct
    18901 cgacgccctt gatgaggagt cgttggttcg gatactgact gagccgcgga atgcgctggt
    18961 gaagcagtat aggaagctgt tcgagttgga cggcgtggag ctcgagttca ccgacgattc
    19021 gctggaggcc gtggctcgca aggccatgga gaggaataca ggcgccagag gattgaggtc
    19081 gatcatggag cacattatgc ttgacataat gtacgacata cctactcgca cagatgtgaa
    19141 gaaggtcgtc ataaccaagg agaccgtggc tgacgacggc aagccaacca tggtgttggc
    19201 cgagaagaag ccggcgcgca agaaggaaga gacggcgtag ggacggtttt cctggggaat
    19261 gcgcgagttg acaagacctt tatagacgtt gctgataatc ccggcgtggc gcatggcgtc
    19321 cgccgggata gttgttgttg gccggggcat gctacaccgt ggacaggagg gatgttcggt
    19381 gagcaacctc ggcgtgattg ggttgataaa cctctttttc ggaatcgtga tcggtcttta
    19441 cttttggaat ctcctcaagg tccagcaggg atcgaaaact gcggttgacc gcgattctcg
    19501 ccgtgaaatg gataagctgc gcgaaatgcg gagcctcagc cttaccaggc cgcttacgga
    19561 gttgactagg cccaagagat ttgaagacgt ggtcggacag aaggatgcgc tccttgctct
    19621 aaaggcggcg ctatgcgggc ccaatcccca acatgtgatc ctctacggtc ctcccggcgt
    19681 cggcaagacg gcggctgctc gggttgtgct tgaggaggcc aagaagagcc cgctttcgcc
    19741 tttccgggcc gatgctgttt tcattgaaat tgatgccaat acagcccgtt tcgatgaccg
    19801 tggaatcgca gatccattga tcggatccgt gcacgaccct atctatcagg gtgcgggtcc
    19861 tctgggaata gcaggggtac cccagcccaa gccgggcgcc gtaaccaagg ctcacggggg
    19921 tgtgcttttc atagacgaga tcggagaact ccacccgatt cagatgaaca agcttcttaa
    19981 ggtgttggaa gacaggaagg tcttcctgga gtcagcgtac tacagccctg atgatcgcaa
    20041 tatccccatg tacattcacg acatattcaa gaacgggctg cctgctgact ttcggcttgt
    20101 tggggcaact actcgccctg cccacgatat ccctcccgcc attcgatcga ggtgcctgga
    20161 gatatacttc aaggcgttga cccctgagga agtcggcatc atagcgtcgc aagctgttga
    20221 caagatgaac atgcgtgtgg aagacggtgt tattcagata gtgaccaagt atgcgcagaa
    20281 cggcagggac gctgtgaata tggttcagct cgccgccggc gtcgccatca ctgagggccg
    20341 aaaaatggtg atgcggtctg acatggaatg ggtggtagaa agcgggcagt attcgccaag
    20401 gccggaacgg aaggtgcccg atgctccgca gataggcatc gtcaacggac tcgccgtttg
    20461 gggcccggga atgggatcgg tgatccaggt cgaggcctct gctgtgcctg cagaggccgg
    20521 gacgggaagt ttcattgtga ccggaatcgt ggaggaagaa gaacaggggg acggctcgaa
    20581 gatcctacgc cgtcgcagca tggcgcggag ttcggtagac aacgtgtcga ccgtcctgcg
    20641 aaaccggcat gcgctgcaag tcggggacta ccacatacat gtcaatttcc ccggaggggc
    20701 gccagtagat ggcccgtcgg cgggaattgc catggctact gccgtgttct ccgctgcaag
    20761 acagataccc atcgacaaca gggttgcgat gaccggtgag ctttcggtgc acgggaaggt
    20821 gaagccggtg gggggtattg tcgccaaagt tgaggccgca gtgagcgcgg gggctacccg
    20881 tgtgctgatc cctgcggaga actggaacga gaggtttgcg gaccgtgcag atgtgcaggt
    20941 aatcgcagtg gaggacatcg atgaggtttt gagaatagcg gcttcctgca atgcagctga
    21001 gaccgaatct gaagcgacat gcacaccttg gcccgaggtt gctgccgcag ctagttgccg
    21061 cagaggggat gcgccgggag gaacgggttc gatgcctccc acagtgcaga ttacagggtg
    21121 agtgtgtctg tgctgctcaa gatgggtgcc gggcgggcat gccaagcttt gcggttatga
    21181 ctgtcgccgc ctcgattgtc cgcgataggt gggcctgggg cggtgacagc cttttgcatt
    21241 gtacatgtgg tagagtatat gcgaaatctg aaagcatcgg ggttgagcgg acaggctgaa
    21301 aacagctagg tgctggcaaa tgggatagaa ttggcacgcc aagtggccag ctgaagagtt
    21361 tcagctaagt caagtcttgt ccccacgcgg gtgggggtgt ttcgccaact ttgcaaatca
    21421 aggttgccca ccagtctcgg agtattatgg tgacgcagat cgctccccta atccccaata
    21481 tggtatgatt acattacggt cctaatcctt tctccagcgg caaaacaaag ctggcgtgtt
    21541 aacctgtggc tttttgggtg gatatgttgc aatccttact ctctcatgct cccagagtgg
    21601 gctaatctcc aactcgcctg tatccttttc gaatattccg cttatctcca tgaaaagggc
    21661 agtttttcac ttgcccagtt ctgccacgta ttgcatttac cgaggcgctt ttcttgaagg
    21721 aatgcaaaga ggcaaggaga ataggtagaa tgcatgtagg aggtgcttaa tcccttggct
    21781 agagcggaca agttatcaca taaagataga gaaaacgttg cacagagtct gccgcttctt
    21841 cctctcaggg gagttgtggt ctttcctaat actacccttc cgcttgaggt tggaagggag
    21901 aagtcggtgg ccgcgctcga agaggcgatg tcatgcggga agctgattgt ccttgccgct
    21961 cagaaggtcg ctaaggttaa tgagccatct cccgacgaca tctacacggt aggcacagtg
    22021 tcagcgataa agcagttagt aaagttgcct gatggcacta tacgcgttgt tgcggaaggc
    22081 gtcgagcggg ccgttatcca tgaatatgta caatccgatc catgttacat ggttcaagtc
    22141 gaactaatgc cgactgtgga agtatgcgac cgcgagatcg aggctcttgc ccgcaacgca
    22201 ttgcgtgaat ttgagcaatt cgtcaaactt tcaaagaaga tgccggtaga gataatgctg
    22261 gctatcgggg gaacggacga gcccggcagg ctggcggacg atatagcggc tcatatgccg
    22321 ctcagcgtcg aggacaagca ggagcttctc tcgtgcttta gcgttcgaac acggcttgag
    22381 aggcttgcag tgatcctcag acgagaactt gagatcctgg agctcgagcg caagatccat
    22441 ttgcgagtca gaaagcagat ggagaagacc cagaaggaat attaccttcg agagcaaatg
    22501 cgcgctatac agaaagaatt gggtgaccga gacgacccca gctctgaagc cgcagagctc
    22561 aggcagaaga ttgaggaggc caacttccct cccgaagtgc ttgagaaggc cgttcgcgag
    22621 cttgataggc tggagagtat gcctccgatg gcggccgagt ctgtcgtggt ccgaaactac
    22681 ctcgactggc tcctcgctgt tccgtggggt gtaatgacag aggataggct cgatattgag
    22741 cgggctgaag ccatcctcga tgaggatcat tggggccttg acaaggtcaa agatagaatt
    22801 ttggaatttc tcgctgttcg gcagctgtct gacgagttgc gcggacctat tctctgcctc
    22861 gtgggcccgc ctggtgtggg caaaacctca ctgggcaagt ctgttgccag agccctcaac
    22921 aggaacttcg tcaggttttc tcttggcggg gtcagggatg aggcagagat acgtggccat
    22981 aggcgcactt atgtaggggc gatgccgggc aaaatagttc agcttctcaa gcaagcgggc
    23041 agcatgaacc cggtgattct cctcgacgag atcgacaaga tgtctgccga ttttcgcgga
    23101 gatccgtcat ctgccttgct ggaggtgctc gatcccgagc aaaactcgga gtttggcgac
    23161 cactacatgg aggtcacggt ggatttgtcc aacgtgctct tcatcacgac cgccaacaac
    23221 ctgtattcca tcccccgccc tcttcaggac aggatggaga ttataacgct tccgggctat
    23281 acggaagagg acaaggtgca gattgcaacg catttcctca tccgcaagca gttgtcggag
    23341 aacggtcttt cggaagagaa tgtcgttatc caggaagatg cggtgcgcga gatcatccgc
    23401 tgttatacgc gggaatctgg cgtgcgaagt ctcgaacgcc agataggagc gatttgccgc
    23461 aagattgcca gagaagtagt ggcgggggca gaagggcctt tcgtcgttgg ccgcgcagat
    23521 cttcaggagt atctcggtgt gcctcagtat cgttacggcg ttgccgacct tgacagcaag
    23581 gttggcgtgg cggtaggcgt cgcctggact gagactggcg gcgacaccct ggacatcgaa
    23641 gtttcggcca tgaagggcag cggtaagctg atcctcaccg gaaaactggg cgacgttatg
    23701 cgtgagtcag cccaggccgg atatacttat gtcaggtcgc gcgctcgtga acttggcatc
    23761 ccagaggatt tctacgagaa gatggacgtc catatccatg ttccggaagg cgcaattccc
    23821 aaagacgggc cgtctgcagg cataaccatg gccactgctt tggcctcggc tctctcgggc
    23881 gtccctgtga gaaatgacgt ggccatgacg ggcgagataa cgctgcgtgg gagagtattg
    23941 ccagtgggcg ggcttaagga gaagacacta gctgcccata gagcaggcat tcgtacgatg
    24001 ataatccctg acgataacaa gaaggatctc gaggagttgc cggaaaatgt tgctggcgac
    24061 atgacattca ttgaagtctc ccatatggat gaggtgctgg caatcgccct tctctctgct
    24121 aaggacgatg aaaagtcatc attcggcgcc gactacgcag tgggcgaagc aggggacgag
    24181 cagggtggaa ccatgagaga cgtgccattg cctcacgttg ttcccaatac gcctcaaccg
    24241 ccggctgtgt gctgaattgg gcgaaggccc aacatagatt agattaatga tacgatgcga
    24301 actagggcgc cccgacgggg cgccctgggc ttattgtagg acgtatatag cgtccgcagt
    24361 tgctattacc aacaaggaag gcgctcgatg tgcatacaaa aggcaggcct tatgctgcat
    24421 ggaagggcaa ttttacgtgt gcattgcggt gcgtaatcgg gcaggaacaa tggtccaaca
    24481 agataggagg tgctgatatg ccgacaacac gcagtataaa ccatttttcc actgttgaca
    24541 ttgggacaaa ttgagtgtat gcttttcctg agttgttgaa caactatgtc gagcaagtac
    24601 tgaagagagg ttgaatgact catgacatct actgagccta tgaaacctgc cggcgctctt
    24661 tacataggca tagcgtccca actcaaaaat gatatagttc gcggggcctt ccccgacaat
    24721 cgcttgccta cagaggagga gctatcacag cgcttcggtg tgagccgaaa caccattcgc
    24781 gaggctctta gcatgttgga gatggatggc ctcattacta ggcgacaggg tgtggggacc
    24841 tttatagagc gtgataaagt ccatgtgggc tccaggacta ttggctttac cgagagcatg
    24901 agacttgcgg gacgcaatcc aggcaccttg atgatgtcct tcgattggga gcctgctgat
    24961 cagcatatgt ccagcatgtt agatatgagg gtcggagagg ccatgggagt gcttaagagg
    25021 gtgagatcgc tagacggagt gcctgcttat taccagatag agagattgcc ggcgcacgtg
    25081 ataggcgaag acttcgatcc tgacgccatg ggcgaatcac tatttgaata cttgaataag
    25141 cagagagaca tagaggttgg cgggtctgaa ttcagccttc gcgcaactgt tcccaatccg
    25201 gtgatcgccg gaatgcttgg aatggacatt ggacttcctc ttttgatgat agaagatctt
    25261 taccgttcta gagaagataa gccgctgctg tggtcggtga actactacag aagcgattta
    25321 tactgtttcc gagctgttga acgcaacgga acctttgaga tgcttacggt gcaggaagag
    25381 aagatatgac actgggagga atactgatgc agcaagattt caggtacccc aagacggcag
    25441 aagatgtaac agcagagatg ctggccatgc ctcctgaaga gcttgagaag gtctcgcgct
    25501 atccgctgta cattcacaag actcgcgatg atctttacaa gtggattgct aggttgatgg
    25561 ccgatgaact caaggagaat aacgccaaag gcgaacccac ccgctgggtt cttcctatag
    25621 gccctaaggg tcaatacccc ttgcttgcca agatctgcaa cgaggagcgt atcagttgga
    25681 agaacgtgtg ggcattccac atggacgagt acctcgattg gcagggccgt cccatttctt
    25741 acgatagtcc ctttagtttc aggcgctatg ctgatgaaca cttgtacaag ctgattgatg
    25801 atgaattgct ccctccaaaa gaacagatca tatttcctga cgtttacgat atagacgcct
    25861 ttaccgccaa gattgatgag gtgggcggtg tagacagcac ctttgccggc ttcggttatc
    25921 gcggacatct tgcctttaac gagtcgccct gtaacagatg gcagaagatt tcggcagacg
    25981 aattcgccgc cgggaaaacc cgtgtggttc atctgctcga tgacaccatc atcgcgcttt
    26041 cccagcgcat gttgggcgga cattcggatc ccattccgca gatggcgatc actatcggga
    26101 tggccgatat tctgagatcc agcaagatat atctgcttac tgatggggcc ggatggaagc
    26161 agtggatgtt gcgcgtgttc ctgcttacta ccgaacgcga tccacgctac gcagtaaccc
    26221 tgtgccacgg gcataatggg gttatcgtgg gagtagatgc tgcaagcgca gccccgatta
    26281 ccctgggtat cgtgtgattt tgccattgag gagatattga tcatgaccag gcacacaaag
    26341 aaaactgcgc ccacgattac cgtggttggc agctttactg tagacatgat gatgagaata
    26401 cccagattgc ctgtgtatgg agaaaccctt cttggctcca gctttttgca ggcgccaggc
    26461 gggaaaggcg ctaaccaggc agtggcctgc gcgcgtcttg gtgccaacgt caactatgta
    26521 ggcgtgcttg gcatggactc aatggctgag gcagcattgg aactgttcag acaggagggc
    26581 atcggcaccg aatgcctatt tcgcactgac gaggcggcga ccggtgtcgg cttcgtgctc
    26641 cagtttcctg acggaaacaa tgtatgcctc actgacatgg cagccaatca gcatctcagt
    26701 gctgatatgg ttgaccgggc cgcatcattg attgaagaaa gcgacgcagt tcttacgtgc
    26761 ctggagattc ctactgatgc ggcccttagg tcaatggagc tggctcggaa acacggtgtg
    26821 tgcagcatac tcaatccggc tcccgctatt gcgggaattg agaagctcaa ggggcacgtg
    26881 gatgtgctta cccccaacca tgtggaagca gccgctctct tgaatataga tccagacgct
    26941 gcgacctcgg cggatgcagt ggccatagcg cgtacactta gagaagaagg cttccctgcg
    27001 gtgatcatca cttgcggaga agatggtgcc gtaatcgccg acggctgcgg tgaaatgaaa
    27061 gttttaggcg caaaggtgca agctgttgac actacaggcg ctggcgacac cttcaacgct
    27121 gccttggcag ttgcgatcgc tgaagggaag ccgcttgctg aagcggtgat gtttgccaat
    27181 tgtgctggcg cattagcctg catgaaagtt ggatgtgttg cagctatacc ttacagagat
    27241 gaagttgagg agcggtttag atcgacctac tctgcgatga actagggggc tgttcggatt
    27301 gtccgatgaa tacgtgttgg aaatgaggca gatctgcaag cgctttggcg gcacgcttgc
    27361 ccttgacaac gtcgactttg cggtgagcaa aggcgaggtg cacgccctca tcggggaaaa
    27421 cggcgctggt aaaagcaccc tcatcaagat actcagtggc atttactctg ctgactctgg
    27481 aactattgcc gttgatggcg agacggttga gatacattcc cctcaagatg cacagcgact
    27541 cggaatagcc acagtgcatc aggaattgaa tcttgcccct gacttgacag tggcagaaaa
    27601 cgtgtacctt ggcaggctgc caacgaagag aggctcgatc atagattggt ctgcattgaa
    27661 aaacgaggcg gaactcgcct ttgagcagct gggcgtggct gaccagatcg acccgatggc
    27721 gccagtgcgc agcctaagca tttccaaaca acagcttgtg gagatagcca aagctttctc
    27781 cgctggagcg cgtcttatta tcatggatga acccacatcg tccctgcctg aaagtgaagt
    27841 tcagcatctg tttgatgttg tcagaaccct aaaccgcaag ggggtcagct tcatctacat
    27901 ctcccaccgc ctcgaagaag tattggccat taccgattcc atgactgtgc tgcgcgacgg
    27961 tagattggtc ggatcgtgca agactgccga gacatcctat gacgacatcg tcaggatgat
    28021 ggtggggcga gagcttacag aacgttttcc taagagagaa ccgagtatag gtgagactgt
    28081 gctcgaagtg gaaggccttg ctgatgctgg acgttttcga gacatatcat tccaggtgcg
    28141 cgccggagaa atcttgggta tttcaggatt gatgggctcg ggccgcacag aactggcaca
    28201 ggcaatattc ggcgtagtcc ctcctgaaga gggaagcatt cgtctcaatg gcaaggaact
    28261 caatgttcgc tgtgctgaag atggcataga ggtcgggata tacctcgtgc cggaagacag
    28321 aaaacgtcat ggtctagtgc agacgatgtc tgtcaggtcc aatactgttc tctcgattct
    28381 gagcaggctg tcgggggcgt ttggggtagt agacaaaaac aaggaagaac aggttgtaac
    28441 gaaggcagtt cgagacttta acgtgaagac agcttcgata gaccagaacg tatcgagcct
    28501 gagcggaggc aatcagcaga aggttgtatt tgctaaggct gtgtgcagcg gaccgaaggt
    28561 actcatactc gatgaaccca cgcgtggtgt tgatgtgggc gcaaaggctg agatatacaa
    28621 gttagttcag gatttcgctt cttcgggagg ggccgtggtg ttcatatcgt cggagatgcc
    28681 tgagattctt ggcatgtgcg acagaatact tgtgatgtct gagggtcgaa tagtaggtga
    28741 atttgcggga tccgaagcaa cgcaagaaga gattctcaaa tgcgctctcg cgaagtgacg
    28801 acagaatacg gggtgtaaat gatgcgacgc ggagtaatgc agaaataccg gattctattc
    28861 gtgtttgtag ggatatgcat cctgcttagt gtgctgaccc ctgtcttcct ttcagtgaac
    28921 aacataataa acgtgatcag gcaggtatca ataaacggga tactggcggc agggatgaca
    28981 atggtgatca tctcaggagg cattgacctt tctgtcggat ctgtggctgc catttgcggg
    29041 gctattgttg cgggaacgca gcttaagctg gggcttgtac ccgcaatgct gcttagtgtt
    29101 gctacaggcg cattgctcgg cctgattaac gggcttgtga ttaccaaagg caaggtggcg
    29161 ccgttcgttg ccacactggg aatgacgacg gtcgccagag ggcttaccct catctatacc
    29221 ggaggacgtc caatatacaa cctcacagat gccttcagag tgctaggagc aggttatgtt
    29281 ggcccgatac cagttcccgt tctcatactg gcactcgtaa ttgcagtagt gcatttcgtg
    29341 atgtcgtcta cggttttcgg acgtgaagtc tatgcgctcg ggggcaatga ggaagcggct
    29401 cgatactcag gaatcaacgt agaacgcagg cgtatgctgg tctatatctc catgggcttg
    29461 ctgtcagcgg tcacaggaat agtccttaca tcccgcttgg gctctgctga tcccacggcc
    29521 ggagtcggtt ttgaaactga cgccatcgcg gctgtcgtta taggtggaac aagtatgtcg
    29581 ggaggagagg gctccgttat cggatctctc attggagccc tgatcatcgg cgtcatgaac
    29641 aacggattga accttttgaa tgtgtcgcca tactatcagc agatattcaa aggcctcata
    29701 gtagtccttg cggttctcat ggattcggct tcgagacgca aacgtcaggc ggctgctttg
    29761 aaggttggca ggtagtgtca gaatgggagg gatgaagagc tcagattaaa ccataatgta
    29821 gagtcgattc cacaggctgt tttgtctagt cactcaccaa tcaagttttg gaggtgccaa
    29881 cgagatgagg ttttcaacga agcgtacaac acaggtcgtc atcggcatta tcatggtggc
    29941 gttgtgcatg gttatgaacg taggcggcgc agcggtagca gccgcggcca aacctcgaac
    30001 ggttgcagta gcgttctgca ccctgaacat accgttcttc gtggaaatgg agaatggtat
    30061 tcgcgcggca gcggcgaaga acaacgtgaa gctcatcgtc actgatgcgc aggatagtgc
    30121 ggcaaagcag ctttcgcagg tggaggacct gcttgttcag aagccggatc tgctcctgat
    30181 tacccctgtt gactcagatg tcctggttcc tgcagttgag gctgcataca aagccaaggt
    30241 gcctgtgatc acaatcgaca gaagcgtcaa ctcggacaag gtagtctcgt tcataggcgt
    30301 agatgatgtt gagggagggc gcatggcagg ccgctacgta gtggagaggc tcaaaggcaa
    30361 gggcaacgtg gtaatgctcg agggcgtcat gggcagctct gcccagcgcg accgcgcgca
    30421 aggctttgag gaggtcattg gaaagaacaa ggggatcaag atgctcgcca agcagaccgc
    30481 caacttcaat aggggcgagg ccatgaccgt gatggagaac tggctgcagt cgttccgaac
    30541 gattgatgca gtgtttgccc agaacgatga gatggctttg ggcgccatcg aggcaatacg
    30601 ggccgcaggt cggcagaatg aaatcatcgt cgttggattc gacgctgatc ccggtgccaa
    30661 gcaggccgtc aaagaaggca agatggcggc cactattgaa ggctcgccat acaaacttgg
    30721 cgtaatgggc attcaggccg cagtggattg ccttgccggc aagaagcttc caaagcaaat
    30781 attgattccg cttgacctga ttacgaagga tacgttcaaa tagctgtaac tgcatgttca
    30841 taggaaccta gattagtcag cgccctgggg ctgtgcatga tgtgattagc acagccccag
    30901 ggcgcatttc tttgtggcag cacaattgac tcgcatttcc cataaagcgt gaactggtcc
    30961 gatatcgccg aagttgagat gcttgtcgat ttccggcggc atatatgaca atcattgaag
    31021 gagttgatgc cggagaagtc gaatactcat gggacgaata tgcaacgttg cataaactat
    31081 ctgcggcgtg atttctggcg gccatgtagg ttcctgattc gccattgttt cgtatccatg
    31141 accccaattg ccccctagat gggggtgtgc tgacgacttg gtgggcaata tactgaggaa
    31201 gcagtctttc ccacgcaggt gggatgcgcc ttgaaaggac gggatgtgta agacatggag
    31261 aaaacaaaag tgatcatcat gggcgctgcg ggacgcgatt tccacaactt caatgtctac
    31321 ttccgcgaca acgagagata tgaagttgtg gctttcacgg ccacgcagat tccggacatt
    31381 gccggcagaa tgtaccctgc agagcttgca ggaaagctgt atcccaatgg aattcccatc
    31441 taccccgagg accagctcga agagttgatt gtaaagcacg acatcgatca ggcgatcttc
    31501 gcctacagcg atctctcgca tgtcgacatt atgcacaagg cttctgctgt gatggcggcg
    31561 ggagcggatt tccggctgat ggggccgaac actaccatgc tcaagtcgag agttccgatt
    31621 gtcgccattg ttgcggttcg caccggggtc ggtaagagcc agacgacacg ttacgtgagc
    31681 caggcgctga tcgatgcagg ccttaaagtg gtggcgatca ggcaccccat gccctatggc
    31741 gaccttgcca aacaggcagt ccagcgcttc gccacttatg aggatctcga catccatgag
    31801 tgcaccattg aggagcgcga ggaatacgag ccgcatatcg accgaggcgt gatcgtgtat
    31861 gcgggcgtcg actacggcag gatcttggag gaggccgaaa aggaggctga cgtcatcctt
    31921 tgggacggtg gaaacaacga cttctccttc tacaagcctg acctcaccat taccttggtc
    31981 gacccacacc gggcggggca cgaggttctg taccatcctg gcgagaccaa tgtccgcctt
    32041 gctgatgtgg tgataatcaa caaggttgac actgccaagc cggaagatgt cgaaacggtc
    32101 cgcaagaacg tgcagaggat caacccgagg gctcagatca tagatgcggc ttcgcctgta
    32161 acagtggaag atcctgagat gatccgcggt aagcgtgtgt tggtagtaga ggacggtccg
    32221 acgctcaccc atggcggaat gggttatggc gcgggagtgg tagcggcacg ccagttcggt
    32281 gccggcgagc ttgtcgaccc gaggccgttt gccgtaggca gcatcgtcga cactcttgag
    32341 aggttccatc atctcgagcc gttgctccct gcaatgggat acagcaaaaa gcagatagat
    32401 gagcttgaga ccaccatcaa cagatctgat gccgaagtgg tgctgattgg cactccgatc
    32461 gatctccgga aggttctcaa gatagacaag cctgccgtga gagtcaggta cgagctcgaa
    32521 caggagtccg gccccgacct cagggatatt gtgtgttcca agttcgctaa ggagtagatg
    32581 tttgggcggg ccccggccaa aatggcatgc cggggccgta caagctatgg cagatatgga
    32641 agcaagccca catggtatca catagtttac agtgtagcct taaaatggta taatcctgtc
    32701 agaatcaaca ctgacaggag ggacggttta tgcgtcggag ctcttgcgcc accaggcata
    32761 aagctttccc atgtgtgctg gcatttacga tggcggtgtt cttctgcccc ccatggcagg
    32821 cggcgggcgt gtctgccgcc aatgattccc ttgtgctcaa agtcgcgctc gactcggaat
    32881 ccttgccgaa gctgactgcc accttgtccc acaaagccgc tcgcttctct tcgacagtaa
    32941 aatcatccac agaaggaacg catgaattca aattggaggc caaaatggct gaagatatat
    33001 cgcatctccg gcagtctgca actttgagca tctcaggcct ccttgctgcg cagggggatg
    33061 cttcctgttg gcgtcagctt tcctccacta ccggcgttgt tgtcgatagg aaagcgagtc
    33121 cgtggcggcc tgaggggaag ctctccataa ccgtgagaga atatccgctc aatgcggcca
    33181 gggactacag cgaaggcaca gtgagggtca aggctgggcg cacatggtcg caaccgtatg
    33241 ctgttgcatt gaaagcggaa gcgaccgcgg gccacaaaga gatgccgggc aatcctaagt
    33301 ggactgcgaa ctactgggaa tcgtctgcaa ccgcgcaatc acctctgggt tcggaatgca
    33361 aaggttcaat cacagtatca gccaaaggcc gggcataccc cgaagccgag cacaaatcat
    33421 atacccgcgc gtcatgcaaa cttgagggtg tttggacccc gtctaaggta cattcgatcg
    33481 actgcagcct cacagcggcg gctacggtcc gggagaagga cagatcttgt gataagaccg
    33541 acacagcgat ttcggctaga tggtcgattg agccttctcg acattggagt gcagacatca
    33601 aagggtcgct ttcatcgtcg attgagccca atagggccga tgccggcagg ccggtgcggg
    33661 caaccggagg agttaccctg acttgcgtgc cagcggacat ctggaggctg actgccgcag
    33721 gagatctgtc attaggccat accttaggcg aaaacgagga tggggacgag gagatcgtcg
    33781 gcattcagtc ggggcaaagc ggcaagctta agatggggct ttcatttgca gcaaagttgg
    33841 gtcccgtaaa cggagtaact ttggacggaa aactcaacct gaagcgcgag agtgagcacg
    33901 caaaagatcg atgggaacac agcacatggg ccccttcggc ggagattcag gcgtctatga
    33961 agttctaagg ctattgcctc agtgctgggt taagtgtaag atactatagt gagtatctgt
    34021 tgcaaacagg cagttaattg cagggctgcg ataaggctta agggaggaga tcgcttgaaa
    34081 aaatataaag tggatcaact gcgcaacctt gccgttgtat cgcatggcgg cgccggcaaa
    34141 acgactcttg ccgaggccat gctatatttg agcggcggtg ttgataggtt cggcaaggta
    34201 gatgaaggaa cttccacgat ggactacgat cccgacgagg tcaagcggaa aatctccatt
    34261 aatgcctcgg tagctcctgt cgaatggaag gatcacaaga tcaacttcat agacactccc
    34321 ggctactccg attttgtggg tgaggttgtg ggcgcgttgc gcgtggccga ttccgtcttg
    34381 gtggctgtag atgcagtctc cggagtcgaa gtcggtacgg agctgatgtg gaggcgcgtt
    34441 gaggaatacg acctcccgcg catgatagtg atcagccgta tggatcggga gaacgccaac
    34501 tttaacaagg ccgtcgaatc tctgacggaa atctttggca acagggttgc tgttgcgcag
    34561 attcccatag gggaaggcga taagttccgc ggggttgttg acctcatcaa aatgaaggct
    34621 tatgtgtggg aggatgcatc gggaaagaag tttgttgaga cggatatccc cgcagaccta
    34681 gccgacatag cagaggaaca ccgcgaaaag ctgatcgagt ttgctgctga aagtgatgag
    34741 gagctcctcg acaagtatct ggagggcgag gctctaactg aggacgacat caaggcagga
    34801 cttgccaagg ccgtgctttc tcgctcggct tatcccgtgt tttgcatatc cggcatgaaa
    34861 ctgattggga ttcagcctct gatggatgcg ataatttcgc tctgtccgtc gccagtggcg
    34921 gttggaccgg tgaagggcat tgtgccgaac tcagaagaca cgatagagcg caagccggag
    34981 gagaccgagc ccttctcggc attggtcttc aagacgatgg cggacccgtt cgtcggcaag
    35041 atcaccatgt tccgtgtgta ttcaggggtt gtgaagtcgg actcgcaagt ttacaacgcg
    35101 agccgcgata aaacggagcg gttcgggcag atattcctta ccaagggcaa gcagcaagtg
    35161 gctgtctccg aagtgggccc tggcgacatt gcaggcgtag cgaagctggc tgatacgact
    35221 acaggggata cgctggccga caaggacaat ccgatcatac ttccaaaaat tcagttcccc
    35281 agttcggttc tgtcgatggc tgtgaagccc aaaagcaagg gcgacgagga caagataagc
    35341 cagggcctcg cgaggcttat ggaggaagat cccacgctca agtacgacaa ggatgcggaa
    35401 tcttcggagc ttgtcgtttc gggaatgggt gaagttcacc tcgacatcat ggccgacagg
    35461 ctcaagagga agttcggcgt agaggtgacc ctcgaagaac cgaagatccc gtacagagag
    35521 acgatcaaga acaccgtgaa ggtcgaaggg aagcataaga agcagaccgg cggtcgtggc
    35581 cagtatggac atgtatggat tgagatgtcg cccctgccgg cgggaagcgg gttcgagttc
    35641 gaagacaagg tgtttggagg cgcggtgccg agacagttca ttcctgcggt ggagaagggc
    35701 ttgagagagg ctcttcccga gggaggggtg ttggccggtt tcccattgat cgacattaag
    35761 tgctcgctct atgatggttc gagccatcct gtcgactctt ctgaaatggc attcaagatc
    35821 gctgcccagt tggcgctcaa gaagggatgt caagatgcga acccgataat cctcgagcct
    35881 atactgaagg tagaggtaac ggtgcctgag gaatacatgg gcgatgtcat gggcgacttc
    35941 aacaagaagc gcggacggat cctcggtatg gagcccgaag gcagatacca ggtggtaaag
    36001 gctctcgtgc caatgtcgga gatggccaaa tactctatcg acctcagggc catgacgggc
    36061 gggcgcgcca cgtttactac ggagttctcc cactatgagg aggctccggc gaacatcgcg
    36121 gcccgcgtta ttgaagaggc gaaaaaggag aaggaagcgt agcaatctgc ctgcgcggca
    36181 gagtgtgcgc atgctatact tgaaaatgat aatggcaaac gcgatgaagg ggaggagtag
    36241 tgcggttaag ctgcaagaga gggatgccca ccgggtggaa ggcgtcctgg cctctgtccg
    36301 cgcgaaggtc gccccggagc ggttggcgtg aaccaggttt gctgcagcct gagtgaggca
    36361 tgcaggcaaa agtagcgtca atcgggactg cccgttacag cttgaaagat cgaagtgagt
    36421 atctgagccg gctcgcccat gacggcaaaa gggctatacg gccctggtcg gtcattctat
    36481 cggctgcatg ccggagggat gcttgctttg aacaaaggtg gtaccgcgaa ggaacgcctc
    36541 cttcgccctt tggtgggcgg ggaggcgtct gtttttcaaa atgtgtcgga cacatcctgc
    36601 gacgcctgca ggcatgtccg cggctacagg aggaaagaag ctcatgggca caacagagat
    36661 atcgactact tacaacccga aagagattga ggaaaagact taccagttct ggctcgagcg
    36721 tggctatttc catgcagagc ccgacccgga tcctgagagg gagcatttcg ttatcttaat
    36781 gcccccgccc aatatcacgg ggacgctcca catagggcat gccctcgacg tcacgatccc
    36841 ggacattctg attagatgga agcggatgca gggggccaac gtctgctgga taccgggcac
    36901 agaccatgct agcatcgcca ctgaggccaa ggtcgtggcg agccttgcaa aggaaggcct
    36961 ctccaagacg gacataggcc gtgagcgctt tctcgagatc gcgtgggagt ggaaggagaa
    37021 gtatggtgga gagatagtgg agcagcagaa gaagctgggc gcgtcgtgcg attgggacag
    37081 gaaacgtttc accatggacc ctgtatgctc caaggctgtg cgcgaggtat tcgtgaggct
    37141 tttcgagaag gggctcatct accggggcga tcggatgatc aactggtgtc cgaattgcag
    37201 aacctcgcta tcggatgtag aggtggagca cgaagacgtt gaatcggccc tgtggcatct
    37261 gcgctatccg ctggctgatg gatccgggca cgtggtagtt gccacgacgc gccctgaaac
    37321 tatgctgggc gataccgcgg tcgctgtgaa cccgaaggac ccgaggtacg ccaagctcgt
    37381 cgggaaaacc ctcatcttgc ccattatgga cagggagatc ccgatcgttg ccgatgccca
    37441 cgtagacccg gagtttggaa ccggggccgt gaaagtcact ccagcgcatg accctgatga
    37501 ctttgaaatc ggcgaaaggc acggactagc ctcggtgaag gtcataggcc ctgatggtat
    37561 catgactgag gaggcaggga agtatgccgg catgacccgg gaagagtgca ggcgccaagt
    37621 ggtggaggaa ttgaagagcg cgggcctgct ggagaagatc gaaagctacg agcatgcggt
    37681 cggccattgc cagcggtgcg gcactgcggt agaacccatg gtgtcgaggc agtggtttgt
    37741 gaagatgaaa cccctggccg aaccggcgat tgaggcggcg aaagacggaa gagtgagatt
    37801 cgttccggaa aggttcacca aaacctatct caactggatg gagaacgtgc gcgattggtg
    37861 tatatcccgt cagctttggt ggggccatcg aatacccgtc tggtactgca ccagctgcga
    37921 ccacatgttc gcctccaggg aagacgccac ttcatgcccg aaatgcggcg gacaagtgga
    37981 gcaggaccct gatgtgctcg acacctggtt cagctcggcg ctgtggtctt tttcgaccat
    38041 gggatggcct gaagaaactc cggagcttaa aagctggcat ccaacctcag tgttggtgac
    38101 agggtacgac atcatcttct tctgggttgc taggatgatc tttatgagcc tggagttcat
    38161 gggcgaggag ccattttccg atgtgctctt gcacgggctg atcaggaatc ccgatggttc
    38221 caagatgagc aggtcgaagg ggacaggtgt caacccgctc gagatcatcg aagagtatgg
    38281 tgccgacacg ctcagattta ctctgatcac agggaatacc cctggaaacg atattaggtg
    38341 gcgtccggag aaagtggagg cttcccgcaa tttctgcaac aagatatgga atgcgagcag
    38401 gttcgtgatg atgaacctcg aggacttcac tttgcccgag ggcaaagtga tgccggaaga
    38461 tctggagttg aaccttgctg acaggtggat catatcgcgc atgaacagag tggctgccga
    38521 catcactgag caactcggaa gatacgacat gggcgaagcg gcgcggacta tatacgattt
    38581 cgtatggggc gagtattgcg actggtatat cgagatagct aagcgccgcc tgtacggaga
    38641 agacgatcca gtcggcaggc gtacagccca gtacgttttg tggaaggtgt tggacggttc
    38701 actcaagatg ctccatccct ttatgccatt catcaccgag ggtatttggc agcatttgcc
    38761 cggctcaggc gagagcataa tgatagagcc ctggcctaca gcggataaag acgagatcga
    38821 cgctgtggcc gaagccgaga tggccgtttt gatggagacg gtgagagcca tcaggaacat
    38881 cagggctgag ttcaacgttg aaccaggcaa gaaggtggac gcaatattcc acgcagacgg
    38941 tgaggcgttc gacactatca acgtcaatgt ggacatcgtt gcccaccttg cgggacttgc
    39001 cagcgtgact gtggagcatg tgtctgcggc caaaccagag aaagctgctg cagcggttgt
    39061 gccagggata gaagtctatc ttccccttgc ggggatggtc gacatcggca aggagaccaa
    39121 caggttgcgc cgcgaggagg agaacctcct gcgcgaggtg gaatccgccc gcgcgaagct
    39181 ctccaatgaa ggcttcgtta caaaggctcc tgccgaagta gttcagaagc aacgcgacag
    39241 actcggggag tatgaggagc agttggtcaa gatcagagct agaatagctc agctagaggg
    39301 ataagtgatg atgtctgaca ggcacactga cgaggtcggg gtccccatgg acgttgccgg
    39361 gggcgaaggc tgggtccgtg ctccctccag cgtctcttcg gagattgtga ggcgcgtgga
    39421 cgaagctctt agacacatgt atggaccgaa gatgctggcg ccaaatggcg atcccgtcgg
    39481 cgagctgatc cacatcatgc ttacgcagaa tacttcggac gcgaattccg accggactta
    39541 cgctgcattg cgaagcgcct attcctcatg ggaggctgtg gctgccgcat ccccgtctga
    39601 gatcgcagaa gtgatccgta tgggcgggct tgcggacata aaggccgcgc gcataatcga
    39661 agtcctttcc aggatagagg cggactacgg ctgcattagc ttggatgcca tcgattccat
    39721 gaatgatgac gaggctttcg attaccttac ttcgcttccg ggtgtagggc cgaagaccgc
    39781 cgcgtgcgta ctcttatttg ccttggggag gccagtcttt ccagtagaca cccacgtgca
    39841 cagggttgca aaccgaattg gccttgttga tactaaacag cctgccaaga cacaggaaga
    39901 actgggcgcc gttgtgccgg aggacatcgt ctaccagctg cacatgaaca tggtggccca
    39961 tggaaggcga acatgccgcg ctaggcgtcc aaagtgcgaa gagtgcgcct tgcgggatga
    40021 gtgcgcgtgg gcgatagctg caagctgtaa agcaggagat aaaggcgttt cggaagaatc
    40081 agtcgccgag cgtgtggccg aaggcggaag agcttgaaga gggtttgctc caatgctcat
    40141 gtattcttcg agagttgttt tatgaatgtg aggcatggag tagatggatt atgaagcagc
    40201 cgttgactat ctgagatccc tttcgagatt tgggatcaag ccaggattgg aacgcactga
    40261 ggccttgctt cagtcgatgg ggaacccgga gcgaagcatg gctcacgtgc acatagcggg
    40321 aacaaacggc aagggttctg tttcggtgat ggtcgaatcc gcgctggaag aggccggaca
    40381 tgtttccgga ttgctcacat cgcctcacct gcagcgctac acggagcgga taaaggttgg
    40441 gggcagggag atttcccagc agcagctcgc tgaattagtt ggagagatgg ctcccgtcat
    40501 cgccaagctg agcgagaatg agaacatagg gactcctacg gaattcgagg cgctgactgc
    40561 agcttgtttt gagtggttca tgcgctccgg tgtggagata gccgttgttg aaacaggcct
    40621 aggcggtacc tatgactcga caaacgtggt gacccctgaa gtgagcgtga ttacccacat
    40681 agcaatggac cacatggatc gactgggcaa caccctttct gagatcgctt ccaacaaggc
    40741 cgggatcatc aagccggggg tgcctgtagt cgtgtcgcca caggatcccg aggctatggc
    40801 cgttattgtg gccaaggcga acgaagtggg ggccgaggtt acggttgtcg gaatggatgt
    40861 cccttacgag cctatccata tggatctaga cggcacgact gtggatgtgg atgctcctgt
    40921 cctcggtcgt ttgcgtgttc acactcccct tgtcggacgt catcaagtga ctaactgtgc
    40981 aacggcagta gctgctctag atgttcttga aggactgaaa ggcgggacta tctcacctgc
    41041 cgcggtttgc agcggaatag ggaaggcgtg ccatcccgga cgattcgaag tcgtccgcaa
    41101 gtcgccatat gtcgtgattc tggatggtgc tcataatctg gatggggcgg ccagccttgc
    41161 ggcgacgctt cacgagattg cgtatgggct gaggccgagg gtgatggtgt ttggcacgtc
    41221 ccgcgacaag cctgtccgcg agatgctggc agcacttgtg cccgaggtgg atgtggttat
    41281 cgccactgcg gcggttggcg ctaggacagg ggcgcatgat cctgagttcg tggctagcgt
    41341 tgccagagag atgggggtcg aggcgcatgc agtcgcttcc gcctgcgagg ctgtgcaggc
    41401 cggagctcgg actgcatctg cgattggggc aaagtcattg ctggtggcag ggtcgttgta
    41461 tctggtgggc gaagtaaggg ggatatggag ggactagatt tgacgcaggg gtgggcagag
    41521 atggagaagt tccgaaaggc ccgcgttact gtgctcgtgg gggcctttgg atcgggaaag
    41581 accgaactcg cgctcaactt cgcgaagcgg ctgaacgccg cgggacgaaa tgcggttctc
    41641 gttgatatcg atattttaaa gccgatgttt cgttccaggg acaaaagaaa tgacctatca
    41701 gagctgggag tgcgtgtggt atcgagcgtc actgattatg aggattccga tgttcctgca
    41761 ctttcgcctg ctatatacgg ggttatagag gacaggtcat cagacgtcgt ggtcgatgtg
    41821 ggcggtgacg atgatggcgc gcgggcgctt gggaggtttc gcgaggcgct cgaagaagct
    41881 gggtatgaaa tgttgtatgt ggtcaatacg cgcaggccct tctcgtcaac tccggaggag
    41941 atagtctcca tggtgctggc ggttgaggag gcgtccagac tccgatgcac tgcattggtg
    42001 tcaaacacta atctgggttc ggagagcttt gttggactgg tggaggaagg actcgatatt
    42061 acgctgcagg catcacaaac cttgggagtg ccagtgcggt ttgcgatgat ccaaaagagt
    42121 gttgctgatt cgcaaaggaa tgagttagag aagatcgtgc aggtctacgg ggtgcctgtg
    42181 ttcgaaatgg atcgatcaat tctgcctcct tgggaaggaa ttgcacagtc atgagtggta
    42241 ttacacaaga atagaagtat ctactgaggc ggggccatgc cccgtaccgg atgaatcgag
    42301 caaaggaagg gatatcacat gccggcgaag cgatcgtcct caggcaatgc tctgtcatgg
    42361 atccttgcag tggtcttgat aagcgtggcc gcggtcgggg ttggctggct catcgggcag
    42421 caagtgctgg gctggattaa cccgaaccga agctcagtat ctgaagaaga ctcaggagat
    42481 actggaggtt acagctatta tccatggagc gggtcggatg gagcaggaag tgcgtcccag
    42541 cagccacagc agacgcctgc tcccaacatc aatctgccca caagcagtct gcccgaaagc
    42601 agcgcggttg agccccctac atacggggca gaaagctcat cagtacccag ttctgttccg
    42661 gatggcattg gttcgacaca aggcacggtt tccagtcccg ttgctgctga gttttacaag
    42721 gtacgtgtgg gttctttcgg cacaaaggag gctgcggcgg ccaccgccag ggagctcgaa
    42781 gctcaaggtt atccgatatt cgtcacaggt ggtggtccat ggtctgtcca agttggagcg
    42841 ttttcgctta gggagaatgc cattgtgcta ggcgacatcc ttgcaaatca gggctacgac
    42901 gttacaatag ttgaatagct gtgcgcatgc catttccagg gctaatgaaa caggcgttgg
    42961 cgcgtggagc gtccgccatg ggcggcaacg atcggttatg cgcggtagtt tgcggagcga
    43021 tcagcaataa tgatacaggc ccggtccggg cgcaaaaagt gtgggccggg ccaggcgctt
    43081 ttcggatgag tgtcgggtcc acagtcttga atacgccgct ccttgagtag gagcgtagag
    43141 tgaaacgagg gagcgaggct tgaagcttgc agacatccgg gagcttatag gggcggaggt
    43201 ccttgttggg gaggacaagc tttccactgt tgagatcgaa atggcgtgcg gctcggacct
    43261 catcagtgac gtgctggcat tttgccatga caagacgctt ctttgcacgg gactcgtcag
    43321 cgcgcaggtt gttcgcacgg cggagatcgc tgggttgtcc acgataatat tcgttcgggg
    43381 gaaaaggcct gatgagagtg tcatagagat gggcaaggct gccggaatcc caatgcttgc
    43441 aacggatctt ccgatgtttg aaacttgtgg cttgctatat ggcgctggcg ttggcggcat
    43501 ctgccttggc gccgatggga gcaggcgagg agatgtcgga tagtgtatcg cggtggcaat
    43561 gatggacaag ccattgatcg ggctggtgct gctttttccg gggcagccag tcctcactcg
    43621 gaaccggtgc tagagatgag cttccgagtc gaccgatacg atttcgctgc agccggcgag
    43681 gcggccagca agatcaaaca ggttctgagg caattgggca tggcacccga gttggtacgc
    43741 cgtgcggcag tggcagcgta cgaggcagag atgaacctag tcatacactc ggagggcgga
    43801 cagatccttc tccgtgtgga cacggagaag gtgacgcttg tcgcttgcga tggaggaccg
    43861 ggaatgcccg atgtttcctt ggcaatgcgc gaaggctact caacagcgcc ggatgagata
    43921 agggagatgg ggtttggggc cggaatgggg cttccgaaca tgaagaggtg ttctgattcg
    43981 ttttcaatag aatctgaggt gggacgcggg acgactgtga ccgcagcaat caggatagcc
    44041 taaagaagca gagcagccaa aaccgtggca gttcgcgttc catggggagg tttaaatggg
    44101 catggagggc cggttccatt ctgtgaaact cctcgaggaa aaatgcaaag gctgcaccaa
    44161 ctgcattaag cgttgcccga ctgaggcaat cagagttcgg gaaggaaaag ctgtaatcaa
    44221 cgctgcccga tgcatcgatt gcggagagtg cgtgagaacc tgcgaaaacc acgccaagac
    44281 cattatcgct aactccatcg acgacctcaa aaactacgat tatactattg ctctaccggc
    44341 gccggctctg tactcgcaac taggccgggc ctcaggccct gacgaggtgg tatgggctct
    44401 tcaggagatc ggctttgacg cagtgtgcga cgtatccttt ggcgcagata tcgttaccga
    44461 gtgtacgcgc agacacctaa tatcccatcc taagccccgg ccggtgattt ccgcggcgtg
    44521 tccggccgta gtccgcttga ttcaggtgag ataccccaac ctcatttccc acgtagcccg
    44581 agttgagagc cctatgtacg ctgcggcccg tgtagcccga gttgaagccg ccgagaagct
    44641 cggcatagcg ggcgatcgca tcggcgtgtt tttcatctct ccttgtgcgg ccaaggtaac
    44701 agcggttatt gccccattgg gatcagagtt ttcgtacgtg gacggtgcga tttcgatatc
    44761 agacatatac ggcgaactca tgactgagct aggacgagga cgcagcgccc ggcccatgca
    44821 cagagggtcg aaccttgggt ttgcatgggc aaacagcagt ggagaactga aggcgttggg
    44881 gcttcataac gtcttggctg tggacggaat acacaatgtt ctgggactcc ttgaagaggt
    44941 agacaacggc aggttggcag gggtggacta catcgaagcc ttggcatgtc ctgcggggtg
    45001 tgttggaggt ccggctacag tcgagaaccc cttcgttgcg cgggcgcgta tgatcgagat
    45061 ctcaaaagct gtggaggctg attccgctga agctctctcc gaggcaaggc ggctggtaga
    45121 tgagcgcggg acagatttct tcgctctcca gggcgagctt acggccctct caggtcttga
    45181 gatcgatccg cgcctcgaag tagccattgc caaaatgagt gagctggagc gagttttggc
    45241 tcggcttccg ggcttagatt gcggtgcctg cgggtcgcca acttgccgtg cccatgcaga
    45301 agatgttgtg tgcgggaatg catctgaagt agattgcgtg ttcaagctga gagaggccat
    45361 ggaagaggtg gccgaggagc tcctcagatt ggccaagacg gatcttccgt cgctgcagag
    45421 gaggaacgac aagtgactgt tcgggagata gctgccgttt tgggaatgaa atgcgtggcg
    45481 ggaggcgtag ggctggacag ggaggtctca tccggatacg tttcagattt gctcagcgat
    45541 gtcatggcga atgcaagtga tggcgggata tgggttactt cacaagttca ccagaatgtt
    45601 gttgctgttg cgctcctgct cgatctcgca gctgtcgtga tcgcaggggg gcttgagatg
    45661 aatgaggatg ccattgccag ggcagaggcg gaaggcatgc caatcctgtc caccaagatg
    45721 tcggcgttcg agacagtcgg cagactatac gagctgggcg tgaggggaga ttcctaggga
    45781 tggttcaggg cttcgccgcc gatctgcaca ttcacacttt cctatcgcca tgcgcgggca
    45841 gggagatgaa cccgaaggca atcgtggggc ggtgcttgga cctagggctc gacataattg
    45901 cctctgcaga tcacaactca gccgccaacg ttgaggcttt ggctgcggcg gcagagggga
    45961 ctggacttac cgtcatctgt ggcatggagg tttgcacaaa ggaagaggtt catctgttga
    46021 cattgctgcc agatttgtct gccatgcatg aatgggagct gatagttgca ccccatagag
    46081 ctaccggcag gaacatcccg gaaatattcg gaaggcagtg gatttgttcg ccgcgcactg
    46141 gagatatcac agaggagacc gcgctcttgt ccgccccaac aacgctatcc gtggacgaag
    46201 tcgtggatgg cgttgacgcc ttggggggca tttcggttcc atgccatatc gacaggcgtg
    46261 ctttcagcat cgtagcccag ctaggatttg tcccggacca cctcgacatt cccgtactgg
    46321 agatatccag gacgattacg gcaaccgagg ccagagcgaa gtttcctgcg acgcaaggcc
    46381 gcgtcctgct ctcttcctcc gatgcccaca ctttggaaga aatcggattg ggatgctcgc
    46441 tcttttggct ggaagaaccg acatttgctg aattaattct tgctcttcgc ggagaggcag
    46501 gaagatacgt gcaagtggag taaaggcgat tgcttatagc ggattttccg tgctgggcag
    46561 agtatacaga aatggcggtg tggtatgagg gagctttcaa tgcacatact cgatatagcg
    46621 caaaactcga tagctgccgg cgcagagctt gttgagatcg atgttatcga ggatgtggcc
    46681 aacgacaaga tgattatcag ggttgccgat gatggccggg gaatgcctgc cgacgtcgtg
    46741 gcgcagatcc gcgatccatt cgttacatcg aggactaccc gccgagttgg actggggatt
    46801 ccgctattgg ctgcagctgc tgaaagatgc ggtggcggag tggacatagc gagcgtgcct
    46861 ggcggtggca cgactgtaac agctattttt gagtattctc acgtcgaccg ggcgcccttg
    46921 ggcaacatgg cagaaaccct tcttgcattg gtagtatgca acccaaacct ggatttcgtg
    46981 tataatcatg ttcgaggaga taattcattt cattttgata cccgagagat tcgattggag
    47041 ttgggcagca tccctctgtc ggacccggag gtcgtagtgt ttattcgtga tttcgtagca
    47101 gagggcgaaa ggaggctgga tgcatgaggc agtttggcat tgtgaagcgg gtttgcggag
    47161 aatccgtgga agtcctggtc agccgtccgg cagcctgtaa gggctgcgga gcttgccaga
    47221 tggcgaataa tcgtccccat accgtgattg cgagaaacga tgcctcggcg gaagtaggcg
    47281 acacagtggc attggaactt cccggacaag atctcatgcg cgcagtcctt ctcgcctatg
    47341 gagtgccatt ggtcatgttc cttgccgggc tgggagtggg tgcgcttgca gcccggagct
    47401 tcggatggac gatagactcg tctcttgctt ccgctatagg gggaattctc tttctagctg
    47461 ccggctacta cggaattcac ctctacgatc gttctcttgg aacggaagct tttgcatctg
    47521 ttgccgtggc tgtggtggat tcgaacaacc ctgagtgtgc gtcgattcat tcaggggctg
    47581 ggcgggttga ggatacggag gagaccgaat gaagcaggag aagataccag atgtagttgt
    47641 aaggaggctt ccactgtacc tacgcgcagt ggaagacttt gatcgcaggg aacatgcaat
    47701 cgtctcatct agtcagctag gggagtggac agggttgact ccggcgcagg ttagaaagga
    47761 ccttgcgttt ttcggagaat ttggcaagca aggcattggt tacaatgtaa agttcttgca
    47821 tgaacaacta agggagatac ttggccttac cacagatgtt cgcgtgggcc tggttggcct
    47881 gggaaatctg ggccgcgcct tgctccatca taatgtagat gctcgcatcg acggctcagc
    47941 cgtagaaggc gaccctgccc gttgggtccc tgaaagcctg cgtattgtcg ccgctttcga
    48001 tgtggaccca caaaaagtgg gtattaccta tgcccaggtc ccagtctacc acgtagatga
    48061 gtttgctgcg aaagtccgcg agctcgacct gaagattgta gttattgcag ttacgcctga
    48121 gagcgcggga gctgttgcta gcgaatgcgt gaaagcgggg gtcaaggcta tcctgaattt
    48181 tgcccctgta aagcttgatg tgcctgaaga tgtaaaggtc cattcggcgg acctcacgtt
    48241 ggagctacag agccttgcgt tttatactcg ctgagcatgt gcgaggaatg tcgcaaggcc
    48301 agcgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaataa gcagttggct
    48421 gtcaaaccat gtgaaagccg ggcggccgcc cggctttctg catggtcggc gacagctgca
    48481 actgcgggac gggaagcttg agtgccacga ataggtgtta gactgtgctg agagcccatg
    48541 tatgtgcaat aatcggtgct cttgcattaa ggttgtcact ttacaccata cacacgaaag
    48601 ggtaggcaat gcggggatag ccttcttcgt attccatgcc gatcatggga tgaggataac
    48661 gccatcatat cggcgggatc agccaaggcc ttgcacttgt tgatcgaata tgttctatag
    48721 gaaatgacgt catggcagga tgcggctgag ctacgcctat cgacggagta gtcgctctcc
    48781 aggggcgaag ggcggtcgaa atgcgcattt cccctaagcc aagggtcttt gtgcgctcaa
    48841 tttcatccca gtctatctat ggtggtggtt ttaacatgga gttgaaccaa gagctgatga
    48901 tcggatcagt ctatgccaga gatgccatac actccctata tggcggtcaa cgaggcttga
    48961 ggatcagcac cccaaaggca gcaccggttg ttcttttgtt tgcaagaaaa agttgcgact
    49021 ttaaggtgcg gcacggcgtc gactgcggct ctcctgaagg agaacacagc tgcgccctgc
    49081 cagaagatga attgccgatc gcccatactg cctcagccga gctggcgcca tacgtttcag
    49141 atccggagga aggatggcgt gatgatggct tctaccattt tcgaggcgag aagcacgttc
    49201 agttccttcg cggtaatcgc gcagtgcgtg accatgctcg tgacggaaag ctgctctttc
    49261 tttttgaaga gactacccgc tcggaaggga gacaggcgtg gagattcatc ggttcgatgg
    49321 aatgtgcagg ctattacttc gcgcaggcgt ccgccgggag gatggcgatc atgttccggc
    49381 ttgctcctgt acacgcgatc gatcgccttc ggaagaattc tcacgaattg gtgctgcatt
    49441 ctgtgttggc agagcgctgc gcgttggatt tcctcaatag ccgccatggc gctattgctg
    49501 cgagcgctga gagcactcgt cccggacatc acagcaatgc ctggaaatgg tcgtcagact
    49561 gcgccctgat tcagcggtat gcgctcgatc gcgctttcgg cttctgtgag agttgcggta
    49621 agccgtcgcc gttttcggac cctgatggtg aaccatatct cgagccccat tacatgcttc
    49681 gaccgtatga ttccggggtg gatcctgtgc atggcatcgc tgcatgctgc cccgaatgtc
    49741 accggatgat ccatgatgcc tggaatgggc ggagtcatga tgtggccctg gcccgcaaga
    49801 tagcactgct tgaacgctca gttgacagag gcgacttcct ggcggttaca gctgcggtaa
    49861 ttaggaacgc agaagggaag gtattgctta ctcagcgagg gcatggcgca cttgccggca
    49921 aatgggagtt tcctggcgga aagatcgagc caggggagac cttggaggct tgtttggcca
    49981 gggaaatcag ggaggaattg aacctggaca ttgcagatat tcgacccctt gtgtcctcgt
    50041 tctatcagta tgatgactct gccgtgcgcc ttttcgggct gtctgccaac gcagttggaa
    50101 accagcttga gctgcgggaa catatcagca aagcgtgggt atggccccat gaattggaat
    50161 cgtatgacct tgcctcggca gatttgccca tagcggctgt tgcggccagg ttattgtgac
    50221 cgtcaagtct ctgtgcccat tgcgctgtag agatgtgcac gcgtgccatg ctcttgagcg
    50281 ggcgttcgaa tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgcgaa
    50401 cttcagttgg ttgaccatgc atgcagccaa ggatattatt agtgctggag cagagtagga
    50461 attgccgcgc ttgcgatgtg ggactatgat ggcagcacag tcagggttgt tgttatgtgg
    50521 gggtgaacgg atgttcaaga ttgttgaaaa gtgcactctc gcgccgggcg tgcaccagta
    50581 tgaagttgag gcgccgctgg ttgctaaagg cgcgcgcccc ggcaatttcg tcgtggtcag
    50641 aacgagcgaa acaggggaaa ggattcccct gacaattgca ggccacaatc gagagacagg
    50701 cacgatcacg ctcgtcgtgc aggaagtggg aaagagcacc atggaaatgg gtatgctcga
    50761 ggtaggcgac tattttaagg atgtagcagg ccctcttggc caggcaaccc gcatcgagaa
    50821 ggtgggaact gtggtggctg tggccggagg gataggggtt gcaccggtcc ttccacagat
    50881 cagggcgcat aaggccatag gcaatcatgt catatccatt gtaggcgcgc gcactcgtga
    50941 tctgctcgtt ctcagagatg aagtggaaaa gttcagtgac gaagtgattt gggccactga
    51001 tgatgggagc cttggatacc atgggtttgt cagtggagcg ctcgaagagt tgatcggttc
    51061 ggggcagaag atcgatgaag taatcgcaat cgggcctgtc aggatgatgc aggctgtagc
    51121 agatgtaact aggaaacaca acatacccac aactgtcagc ctcaatgcga tcatggttga
    51181 tggaaccggg atgtgtggat gctgtagagt cacagtagat ggtgaagtca agttttcgtg
    51241 tgttgatgga ccggagttcg acggacacaa agtggacttc gacgaactcg tatcgaggca
    51301 gggtttcttc cgcgacaagg agcaaattgc aagagagcgc ttcgaggcag ataggggtgg
    51361 gtgcaagtgt cacgcaaaat agtcgacaga actgagatgc cgaagcagtc gccggaggca
    51421 aggcggacga atttcagcga agtggcgctc ggactcgacc cggaattggc aaggcgtgaa
    51481 gctgaacgat gcatccagtg caagactagg ccatgcacga cagggtgccc tgttgaagtt
    51541 gacattccag agttcgtcgc gcttattgcc gaaggtaagt atgacgaggc tgctgcgaag
    51601 ataaaggaga agaacagcct gcccgcgata tgcgggaggg tatgccccca ggagagtcaa
    51661 tgcgagagcc tgtgcacgct ggggcgcaag tatgaacctg tggccatcgg agcgctggaa
    51721 cgttttgctg cagattgggc cagcaagcga gcggttgaga agcgcgaagc ctgtgcaggc
    51781 gatgttgact ccgatttgca gtgcgggtgt gaacagaaag tatgcgagct ggactcccgc
    51841 ggatgcgctc ctacaagcga ttctgcggct tcagcggcgc gtgtggcggt gataggtaca
    51901 ggccctgcag ggctcacctg tgctgcagac cttgccctca agggatacaa ggtagatatg
    51961 tttgagtctt tgcacgacac cggaggtgtg ttgcgatacg gcattccaga gttcaggctt
    52021 ccaaagtcca tcgtcgacga ggaagttgaa tacgtgcgca gcctgggcgt acgaatcttc
    52081 accaacgtag ttgtagggct gactttggat ttgcgcgaaa tgctggagtc tggccaatac
    52141 caggctgcgt tcattgctac tggtgcaggc ctcccgtatt tcctcggcat ccccggagag
    52201 aacctgaacg gggtttattc cgctaacgag ttccttacca gggtcaacct gatgaaagcc
    52261 tacatgtttc cccaatacga tactcctgtc aatattggat cgaaggttgc cgtggtgggc
    52321 gcaggcaatg ttgccatgga ttcggcccgc acttctcttc gtcttggcgc cgaggaagtc
    52381 aagatagtct atcgccgctc tcgggctgag atgccggcaa ggcttgagga gatcgagaac
    52441 gccgaagagg aaggcgtgga gatgatgctc cttgccaatc ctgtgcgcat catgtcagac
    52501 ggctgcggta aagtttcagg gatggagtgt atccgtatgg aactgggtga gccggacgca
    52561 agcggcagga ggcgcccggt gccggtagag gggtcggaat tcattctcga tgtggatacg
    52621 gttattgtgg ccgtgggtca ggggcccaat cctctcttga ctgaacgcac caagggtttg
    52681 gaacttaacc gaaaagggaa cattgaggtg cgtgacgaat gcggagcaac aagcatcccc
    52741 ggcgtgtggg caggtggtga catagtcaca ggcgctgcaa cggtcatatc cgctatggga
    52801 gcgggaaagc gcgccgctag aggaatcgat gaatggatcc ggtctaagtt ttaatggtgg
    52861 aggtgtagct tagaagtgtt gagcgatatc gagatagcgc agtccactaa gctcaggccc
    52921 ataattgacg ttgcgaaaga ggtgggtctg acagaggacg agatcgagta ttatggtaaa
    52981 tataaggcca aggtttccct tgacgtgttc gatcggctga aggaccgtcc gaatggcaaa
    53041 cttatttaca ctacagcgat aacagcgacg ccggcaggcg agggcaagac atgcatggct
    53101 gtgggcttga cccaggcctt ggggaagttg aacaagaagg taatggtttg cttgcgtgag
    53161 ccttcgctgg gtccgacctt cgggatcaag ggaggagctg ccggtggcgg ctattcacag
    53221 gtactcccaa tggaggatat caacctgcat tttacaggag acatccacgc agtaagcact
    53281 gcccataacc tgcttgccgc catgctggac aaccacattc accagggaaa tgatctgaat
    53341 atcgatatcc acagggttgc ttggcgtcgt gtgatggaca tgaacgagag gcagctgcgg
    53401 aatatcgttt gtggtttggg tggcaaggcc aatgggactc cacgggagtc tgggttcgat
    53461 atctctgttg cttctgagat aatggctgtg ctttgtctgg cctctgatat ggaggacctc
    53521 aaggctcggc tcggccggct cgtcgtggcc tacacaaggg acggacagcc ggtgaccgcc
    53581 gcagatctca aggcagtggg ggcaatggct gtcctgctca aggatgcaat caagccgaac
    53641 ttggttcaga cagtcgaagg ccaaccagca ttcgtccacg gcggtccttt tgcgaacatc
    53701 gcccatggca acaactcggt aattgccacg cgcatggcgc tcagactttg tgattacgtg
    53761 gtcaccgaag gtggattcgc tgcagatctt ggcgctgaga agttcttcga tatagtgcac
    53821 agatttacag ggctcacacc ggatgtggcg gttcttgtag cctctgttcg cgcccttaac
    53881 atgcatggcg gcgttccaaa gtcgaaagtc gcagagacga atcttgatgc tctcgagaag
    53941 ggttgtgcca accttgacaa gcacattgag aacctgaaaa aattcgggct tcccacagtc
    54001 gtggcgataa acagattccc tacagatacc ccagaggagc tagagctcgt gaagaagcat
    54061 tgcgatgcct tgggtgttcg cagcgcgata tcggatgttg tggcgagggg cggggacggc
    54121 ggaatcgagt tggcccaaga ggttcttgaa gctgtcgaga atgaccagaa caatttcaag
    54181 cccatctatg atccaaacat gcctattcgt gacaagatcg agatactggc gacggagata
    54241 tatggcgctg atggggtcgt gtacactcca tcagcggaaa aggagatcaa ccgcctagcc
    54301 agattggggt tcgataaaat gcccatctgc gttgctaaaa cgcagcattc gctcaccgat
    54361 gatccgaacc tcaagggagc ccctgttgga tggaagctaa ccgttaggga cgtgcgggtt
    54421 tctgcgggcg caggcttctt ggtgatcctt accggcgaca tcatgacgat gcccgggttg
    54481 cccaaggcgc cggctgcgga gaacgtagac atagacgaga atggaacaat agtaggtctc
    54541 ttttagacgc cccgtggctg tacatttgtg ccagcctaca taaactggag cagcatggga
    54601 aggcgcgctc tcaggcagat tcctgggagc gcgccttccc atgcccgatt gcgggagatt
    54661 gccatgttca agatgtatta ttaccgtgtg ctcagtcaaa cgaggtacgg gactgctttg
    54721 gggtgaagat gaggctgatg tcgtgagctg tcggtatgta tccagattac cttgttgctt
    54781 gccacgaaaa cgactcacca gtgggctatc ctggttacgg tgccgctttc aggaggcaat
    54841 cgggcgggtg tgggatagaa cctttggtcg tctcagacga cgtagcttta cacgttaaag
    54901 acgggcactc ctcatatggg gaatgagcat cagatgcaga tagtctagtg ccggcggcat
    54961 cttcgatcag cttatcgtcc cctatgatcg tgttcagctg ggtattgccc aggttttttg
    55021 ccatgtgcgt gacgcgcaac tgacgccaat ttcgtcgtgg agcgacacaa aaagccttat
    55081 tctcatacta tatatcagtc tgcgggcata gcccgcggat ggaaaacttt cacgggagga
    55141 gcgattgcgg tggaaagcgc agtattcgcc ggcggtttca agggtttctt cgcctggaac
    55201 ctagcattga ttcttctagg cataatagtg atcgcagtgt tctggttccc ttggtaattc
    55261 aaggcatggc ctaaaaagga ggacacgcac atgacagttg gcgatgttat aaaggctgct
    55321 ccagacccag ggccttttgg atgtgggttt ggatcatggg gcatgctcct aatcctgctt
    55381 gccataatcg tattggcagc cttcctgcca agattctgct ggtagaacga ggcagtctct
    55441 gcagtcaacc gtgccgattt tggcacggtt gacccccctg ctttcctgca aaggccacat
    55501 ttcctcccgg ataaagattc tgcagattag tgcccaggcc atcgttcgtc acagggactt
    55561 ttgagttgcc cacacaatta agccggtcat agctgggtaa accctaattg gtgatgggcg
    55621 gtgtgttatg gatgcaagtt gacgcatcgg acgcatcttt ggatcatgga tgcgaagcat
    55681 tgttctgcaa tgattcatac tgtatactca tggtgtggtc actcaacatt tgaaggggag
    55741 tgctggcaat atgaagtcgc ttgatgacct caagaagatc aaggaacgcg ctcaggcaat
    55801 gacggagttg cgtgagggca acgaggaggt aaggatcgtt attggcatgg gcacctgcgg
    55861 tatcgcggcc ggcgcgcgcg atacgctcgc agcggtcgta gatgagatca acacgcgcaa
    55921 ccttcggcat gtggcagtga cgcagacggg ttgcatcggg atgtgtgaat acgagccgat
    55981 cgtggatgta atcaggcaag gtgagccaaa ggtgacatac gggcgtgtta cgccggagaa
    56041 ggctcgccag attatcgcca gtcatgtagt gaatgggcag gtcgtaggcg acctcgtgat
    56101 atcctccaaa taggcaaggg ctttagcgat gggtgccgat agggagagcc tcacgaggta
    56161 tttagttgcg gaactttgtg aaaaacagca caatcttgaa acgcccgatg ttagtaagga
    56221 cagaagggga ggggtccgag catgctcaaa atgcgcggtc ctgttggccc ggaagggcct
    56281 ggtgttgttt gtgagtgcga cgacgtcttc ttaaccgaag tgcagagggt tgtatctgtc
    56341 tacaaggaac gaccggaaga cctagtacaa gctcttcatg atgttcaatc gctccgaaat
    56401 taccttccac gcgaggcttt gcgtgtagta gcgcgcaccc tcgacgtgcc cgaaagccga
    56461 gtttatggag tagctacatt ttactcaatg ttctctacaa gtcctagggg acgccacata
    56521 atcaggatct gcgagtcggc tccgtgccac gtagcggggg ctgctgagat aatcaattcg
    56581 ctcttgaacg aactcggagt ggagatgggg ggcactacat ctgacgggat atttactgtg
    56641 gagacgtcgt cctgtttggg tgtatgtggt gttgcacctg ccatcatgat tgacgacaca
    56701 gtctacggta atcttgtctc ggaagatctg cctgcagttc tgcgccgtta tcgttaggtc
    56761 ccggttttcc gaacgaagct tgggggggat ccacatggag ttctaccgag cgcatgtcct
    56821 ggtgtgcggc ggattcccgt gtgtcgccgc tggttgccgg gcggtgcgtg gtgaaatcga
    56881 acgatcggtc aaggagcatg gtcttgagcg cgaggtccgt attgttgaga cgggctgctt
    56941 aggtccgtgc gaccttgggc cgatcgttgt cgtgtacccg gaaggaaccg tatattcccg
    57001 cgtcagcgtg gatgatgtaa gtgagatcgt aggcgaacat ctgatgaagg ggaggcctgt
    57061 caggcgtctt gaatacacag agcgtcttcc ttcggtatcg caggctgatc acaagcgccc
    57121 cgactacttt gggatccaag aaaggatagt ccttgagaat gtggggctga tcgacccaga
    57181 cagcatcgag gagtatatcg cccgtgatgg gtatctgggc gcgggtatgg ctcttacatc
    57241 tatgtcgccc gatgaagtca ttggcgctat aaaggcttca ggccttcgcg gccgtggagg
    57301 cgcggctttc cccaccggcg tgaaactggg attcactgcg cgggtggagg ctgatcagaa
    57361 atacataata tgtaatgccg atgaaggaga gcccggaaca ttcaaggacc gcctcatact
    57421 cgagggtgac ccgcacaagg tgatcgaagg catgatcatc ttggggtact cagttggcgc
    57481 taccatgggg tacatataca tcagaggcga atactatctc tccatagagc gcatagagcg
    57541 cgcgatcgcg gaggcgcgca ggtatgggct cttaggtcat gacctattcg gctccggctt
    57601 cagtttcgat attgaagtga agaagggcgc cggggcgtac gtgtgtggcg aggagactgc
    57661 gctgatcgag tcgatggagg gcaagcgggg tgaacctagg cagaagcctc cttatccggg
    57721 aacctgtggt ctgtggggca agccgaccgt agtcaacaat gtagagacca tagccaacgt
    57781 cccgcctata gttcgcaacg gggccgattg gttcagacaa ttcggaaccc ctactagccc
    57841 cggcacaaag gtatacaccc ttacaggaga tgttaacaac aagggactga tagaagtccc
    57901 catgggtatt acgctccgtg aggtcatata cggggtgggt ggcggaatcc ctgggggacg
    57961 tcgtttcaag atggctcaga ctggcggaac agcagggggc tgtctgtcag agacgcacct
    58021 tgacatccct atggattacg agagtctcca ggagacaggg tccgctctcg gctccggtgc
    58081 gctcctgata atcgacgaca gccattgcgt ggtggacata gcctgcgcgt tgttgcgctt
    58141 ttttgagcat gagtcgtgcg ggcaatgcac gccttgcaga gagggcacac gacgactgca
    58201 tgacatcttc cgcaagacgc gggagctggc cgcaactgaa aaggatctaa agctggcaag
    58261 ggagcttgca ggggtaatgc aagcctcttc cctttgcgcg ttgggccaat cggttgcagt
    58321 tccgctcttt actatcatgg atcactatgc ggatgagatc gaagctcacc ttgaggggcg
    58381 ttgccctgcg ggcgtatgca ggccaaatcc tgcaaacgca gacgagtcgg catacgagac
    58441 gctccaggcg ggcaacggga tcaggtgaga ggaggcttga cggggaaatg tctgacatta
    58501 ctttgaccat cgatggcaag tctgtctgcg cgtcgcctga gatgacaatc ctcgaggccg
    58561 cgcgcactgt gggaatcaag atcccaacgc tctgttggca tgaagacctg ggaagcccgt
    58621 cggtctgccg cgtctgcgtg gtggagatag agggccagaa cacgctacag ccagcctgct
    58681 cgtaccctgt aagccagggt atgacagtgc gcaccaatac gccccgggtc agaaatgcca
    58741 gacgcatggc catcgaattg ctgcttgcgc accatcctga cgactgcctt tcatgccagc
    58801 ggaacctcaa atgtgagctg caacagcttg cggccgattt tgggataagg gagataaggt
    58861 tcgagcgtgt ttcaaggaag atccccagag acgaatctac tccttcgctg gtgcgagatg
    58921 ccgataagtg cattaactgc agaaggtgca tcgaagcttg tgagaagatt cagggcgtgg
    58981 ccgtgttgtc cactgtcaac cgcggatttg actcggtggt gctgcccgct ttcggtgatg
    59041 atctcgattc tgttgtctgc gtgctttgcg gacagtgtac cttggcctgc cctaccgggg
    59101 ccatagtgga acgggacgat acgggaaggg tctgggatgc gttggctgac cccaatatgc
    59161 acgtcgtggt ccaaactgct cctgcaatca gggcttcttt gggggaggaa ctcggactgc
    59221 ctgctggctc tatagtcact ggcaagttgg tggctgccct caggaagatc ggttttgaca
    59281 gggtatttga tactgacttt gctgctgacc tcacaatcat ggaagaaggg catgagctcc
    59341 tggatcgaat acagaagggc aatgctccgt tgccgctttt gacttcgtgc agccctggct
    59401 ggatcaactt tgttgaacgt ttctaccctg acctgcttcc gcacgtgtcc acgtgcaaat
    59461 ccccacagca gatgtttggg gccgtggcca agacatacta tgccgagcgt gctgacatcg
    59521 accctgctcg aatcttcgtc gtgtcgataa tgccttgcac tgcgaaaaag tatgaatgcc
    59581 agaggccgga gatgagatcg agcgggtttc aagatgtaga cgcggtcctt accactcggg
    59641 aactgggcag gatgatcagg gagatcggca tagatttcga ttcgctgcct gaagaggagt
    59701 atgatgcccc tatggggatt tccacaggtg ccggggccat attcggcgcc actggcgggg
    59761 ttatggaagc agcgctgcgc acggtgtatg ccgtggtgac tgggcgagag ctttccgaag
    59821 atggtctgga attccatgat gtaaggggcc ttgagggtat caaggaggct gaggttgacc
    59881 ttgaaggcgc caccgtgagg gttgcgatcg ctcacgggct cggcaatgcg agaaaggtaa
    59941 tggagacgat caggacgggt gaagctccgt ggcagtttgt ggagataatg tgctgcccgg
    60001 gtgggtgcgt cggaggcggc gggcagccga tcggaactgt gatgtcgagc cgggctgaga
    60061 gaggccaagc tctatacgag gttgacaggg agatgccctt gagggagtct cacaagaatc
    60121 ctgcggtgct cgccctatat caggaattcc tggactggcc actctcggag cgcagccatg
    60181 agctgctgca tacgcattat actccgaaga aatcctgaag agttcggttt tcttgcacaa
    60241 gcttgtttcg tggtgtacaa ttagaccggt tttaggcacc gtggctaaag tgctgttggg
    60301 gtgcgtaaag ccggtctatt caccggacag gctttgttat tttatgcctc acgtgaggct
    60361 ttttgtgctg tgaagttcca agtccatagg caggtttgta gaagcaaaag gagtgaggtg
    60421 catgcccaag ggatcgttcc gagggggagt ccatcctccg gagaacaaag agagaacggc
    60481 cacattgcgc actgttgttg ccaggcggcc gtctcaagta gcaatagcgc ttcatcaggg
    60541 ctccaactgt cccttaacgc ctacggtcgc caaaggagat tacgttcgca tggggcagaa
    60601 ggttgccgat actgatgaaa aatggggggt accagcgcat tctcccgtct caggcaaggt
    60661 tgtctccatc ggcctggtgc gaaccgctag cggcgtcgag gaccaggcca tatttattga
    60721 acctgacgga gaggatgcat tggacgaatc gtgtgtgcct gtcgacaatg tcttttcccg
    60781 gtctcctgaa gagattatca ggataattcg ggatgctggc atagtcggaa tgggaggggc
    60841 ggagtttcca acccatatta agctttcgct gcctccgggg gctaatgtgg atactctgct
    60901 tttgaacggc gctgagtgtg aaccatacct cacagccgac cacaggctga tggtggagcg
    60961 tccgcgcgac attatcaggg gagcacagat catgatgcgt gccatcggtg tttctaggac
    61021 gctcatagga atagaagaca acaagcctga tgcgattcgc gccatggggg acgctgcttc
    61081 aagcgctgca ggcgtagaag tcgtggctgt aaagactaga tatcctcaag ggtctgaaaa
    61141 gcacctcata aagtcggtgt taggtcggga ggtcccacct ggaggcctac cgtttactgt
    61201 gggagtagtt gtcagcaatg ttggcacttg tgcggcggtg tcgaatcgct tcgatcgtgg
    61261 actcccactg attgagagaa tcgtgactgt gaccggatcc gctgtggcca agcctgcgaa
    61321 tttggttgcc aagataggga cttctgccag cgaccttata gaggaatgcg gcggttttgt
    61381 tggccaacct ggaaagatcg tttttggagg cccaatgatg ggcatggcag tgtcgtcggt
    61441 tgatgttccc atcggcaagg gcacatcggg catcttggtc atgagcaggg aagagtcgga
    61501 agacctaaag gaaatgccat gtatcaaatg cggccaatgc gtcgacgcat gtcccatgcg
    61561 tttggagccg gtgttccttc accaatggag caatgcgggc ctgtgggacg aagcagaggc
    61621 ttaccatgct atggattgca tcgaatgtgg tgtgtgcact tatgtatgcc catcccggcg
    61681 caacttggtg caggcgatca agcgagccaa gttcgagatc tcggctcggc gtcgggccgc
    61741 taaggaacgg gggagtaagt gatgggaaac actaagctca tagtgacgcc ggcgccgcac
    61801 ataagatccg gggatagcac gcctgcaata atgcgtgacg tggtgattgc tttgttgccg
    61861 gcagcgatct ctgcagtcta cttctttggt gcgcaagcag ccgtatctat ggtctgcagt
    61921 gttgctggag cggtgggggc cgaatacatc tttgagaaga tcgcaaaaaa gccggtgacg
    61981 ataggggact ggagcgctgc tgtaacaggc ctcttgatcg cgatggttct tccgccgcac
    62041 gtgcctgttt ggataccggt cattgcagga ttcttcgcca taggcgtcgt taagctgctt
    62101 tttggagggc taggccataa tgtattcaac ccggctctcg caggccgtgc cttcgcgatt
    62161 gccgcttggc cagctttgac cactagtggt tatctctggc ctgcatctgc agaaggcgcc
    62221 atctgggctg tgaggggctt tgacgccatg agcaccgcaa ccccgctcac actgtggaaa
    62281 atgggggaaa taggcggagc ttcgatgtcg aagttctatc aatcgctatt tgtgggcaac
    62341 gtagcgggat cattgggcga aacatctgcg ctggcgctgc ttattggcgc ggtttatctt
    62401 ctctgtagag ggcgcatatc cctgagaata cctctttcct atatattgac agtagccgta
    62461 atcacgggcc tatacggtca agacccgctc ttccacgtgc tatcaggcgg gctgatcctt
    62521 ggagcgttct tcatggccac tgactacgtt acatctccga tcaatccaaa gggccagata
    62581 atcttcggta tcggatgcgg gatcctgacg gcgttgataa ggatgttcgg cgggtatcct
    62641 gagggtgtct gttattcgat cctgctcatg aatgcggcag tgcccctgat cgacaggctc
    62701 actcgcccta agcggtttgg ggaggtgacc agccgtgcgt gacgtgctca agctgggact
    62761 tgtgttgatg gtcatatgcg cggtttcggg cgggcttctc gcctacgtgc atggtgtgac
    62821 cagtgagata attgatgaac gaaaggcgca agaggttgcg gacgcaatgc gcgtcgtgct
    62881 tcccgctgcc gagagctttg agaagctcag tgatgacgag cttgcgtcta tcaaggctga
    62941 cccgaagttc gttgacgttg acgaggtata cgagggagct cacgacggca gccttgttgg
    63001 aatagtagcc aaagtgcagt cgcctggata cggaggcaag atcagccttc tcgtgggcat
    63061 ggatccggac ctcaccgtca caggactgca agttcttggg catagtgaga ctccgggact
    63121 tggcgcgaat atcgccaagc ctgagttcat atctgagttt ataggcagag gcggctctca
    63181 tccgcttgct gtcgacaagg acggcggcga gatctccgcc atatcgggag cgactatttc
    63241 gtcgagggga atggtgaacg gcgtcaatct ggtccgcgat cttgtaaatg ccctcggcat
    63301 agctggaggt gttggcagat gagtcctctt aaggccttca ccaacggaat atacaaggag
    63361 aacccaatat tcaaacttct gttaggaatg tgtcctacga tagcggtctc ccttgcagtg
    63421 tcaaacggaa taggtatggg ccttgctgca acattcgtcc ttgtggggtc gaatttgata
    63481 atatcgcttc tgcggaagtt cataccttcc gagattcgga taccttgctt catcgtggtg
    63541 atcgcgactt ttgtcacgat agtcgacctg agcatggaag cctacttgcc ccagctccac
    63601 caggtgcttg gcgtgttcgt tccgttgatc gtggtcaact gcatcattct ggcaagggct
    63661 gaggcttttg cgtctaagaa cgggcccgtg gcatcgatca tggatgggct cggcatgggg
    63721 ctcgggttta cgctcgccct cgtggtgatc tcacttatca gggagcctct gggaacgggc
    63781 gtcatattcg cggcggctga cttggggttt gacggaataa agctgtttcc cgccgagtat
    63841 gcggcgtctg tggtgacaca gcccgttgga ggcttcatca ctctcggtct cttgcttggc
    63901 ctagtcaatc tgatcacgga taagaaaaag gacaagcaag gctgaaaggg gtgtttgtag
    63961 gtgcgtcagc tgctggtaat cttgtttagc caggcttttg tgaacaactt catgctctcg
    64021 aggatcctgg ggacatgccc gttcataggc gtatcccagc aggtagagac tgcaacaggt
    64081 atgagcctcg ctgtggtgtt cgttatgact gtggcgtctg ctattacatg gctggttcag
    64141 aatttcatcc ttaacccgct taacatggca tatctccaga cagttgcatt catattggtc
    64201 attgcggccc ttgtgcaact catagagatg gttataaaga aatccagccc ttctttgtac
    64261 cgggctttgg gcatatacct cccgctcatc actacgaact gtgcggtgtt gggtgtggcg
    64321 atcatcaact accaggccaa ctacaatctg ttggaatctg cagtgagcgg cgccgccgga
    64381 gggctcgggt gggctcttgc gattattctt tttgcggcca ttagggaaag gcagagattg
    64441 gcgccgatac cccgggccat ggagggattc cccatagccc ttgtaactac tgggctgctc
    64501 gccatggctt ttctgggctt ttcggggttt gatgttgcca ggttcatggg gttgtaggcg
    64561 cgacgtttcg actcatggtt tcgctcgtag atgggggtga catagatgag cagtcttaat
    64621 gcgttgttga gcatgggggc tatggggctg atcttcggga tttcgctcgg ggctgcatcg
    64681 aagaagctag cagtagagca ggatccgagg atcgatcaga taatggaagt gctgcctggc
    64741 gcaaactgcg ggggatgcgg atttgcgggg tgttctgcgc tcgcgggcgc tattgtgtcg
    64801 ggcgatgcgc ctgtaacagg ttgtcctgtt ggaaagagcg cggtggcaga gaagattgca
    64861 aagataatgg gagtagagtg cgcaacgact gagcccaagc atgcaagagt cttgtgtcgc
    64921 gggagtcgca aaaacgcagc ggagctctat gaatacaggg gacttcagga ttgccgatcc
    64981 gcccagctca cagggggagg cgccaaggta tgcacctatg gctgtctcgg ctttggctcg
    65041 tgtgttgcag catgccagtt tggagcgatg tcgatggggc ctgacgggct tcccgtagtc
    65101 gatgattcgc tctgcactgg atgcggaaag tgcgtgggtg tgtgcccgcg agggctgatc
    65161 gagcttcgtg ctgcggggga gtctgtggac gtggtgtgca aatctcacgc gcgcggcccc
    65221 gtggtgcggc aggcgtgttc tgtggggtgc atcgggtgtg gactatgtgc caggaattgt
    65281 cctgagaagg ccatagagat cgtgaacaat ctcgccgtta tcgaccaggc caaatgcacc
    65341 agatgtggaa tttgcatcga gaaatgtcct cagaagtgca ttgtggattc tgctggtgtc
    65401 gaaggaatta cgcccgcaaa gaaagatgag gccgcagtat aggcgatcgc tgtttgtgtt
    65461 tgccaggacc agcctctgtg ctcaagtacg gattgatcag tgttttgatt gacgcggcct
    65521 cggtcaaagg cttgtccatg ggcggcgcaa aagttgtgga cgggcttcca tctatgggag
    65581 cccgttctcg tcattccagg tcttgatcga tgcgacattc ttgtaaacgt agaattcaaa
    65641 tggtagactt aataaaggta aagagggccc tttgggcggc gccgcctttg gcatcgctct
    65701 tgtcgggcgc ggggagtttt gatgtcgtga gtaggctgat tttggcatct gcttcgccaa
    65761 gacgttctca gcttttgcac gggattgggt tagatttcga ggtcatcccg agcaacgttg
    65821 tggaggaaga agtgaacggt gccgaccctg cagatctcgt tatgcgactt gcgtatgcca
    65881 aggctgctga cgtggcaggc gggatcgatc atgggctcgt gatcggtgcc gataccatag
    65941 tcgtcgcgga cgggcatgtc cttggaaagc cggtggaccg ggatgatgct atccgcatgt
    66001 tgcacaaact ctcagggaag gaacaccagg tatactcggg cgtttcggtg atagatgccg
    66061 gaagcggcaa ctcggcaact gagtatgaga tgacaaatgt caagtttagg actgtttcgg
    66121 atgagaaaat agcaagatat gtcgataccg gcgaacccat gggcaaggct ggcgcttatg
    66181 ccatccaggg aattggaggt ttgctcgtgg aatcgatcag gggcgactac aactgcgtag
    66241 ttgggcttcc attgggccgg cttgcgctga tgcttgagag ttttggttat gtagtttttt
    66301 agttgcgcct cgccatggca acgcttgcac acaaaatgca ggtgaggtgc gataatgaaa
    66361 caggttgacc ctggacggtg cgggatgggc ctcagcatca gggagatgcc tcccagcgaa
    66421 aggccgatgg aaaggttgct cgagcttggg ccggaggccc tttccactgc ggagctgata
    66481 gctgtgataa tcaggtgcgg gcgcccgggg gaaagcgcag tcgatgttgc ttcgcgggtt
    66541 ctctcagaat gcggtacggc gagaaaacta gcgacagcag gcgtatgcga gctatcgagc
    66601 ataccaggaa taggacccgt tcgagcggct cagatagttg cagccatcga acttggcaga
    66661 agggtcgtag tgtcggaagg ggagccaagg ctttctatat ccagcgcgaa agatgtagtg
    66721 gacctgctca tgccgtcaat gcgttatctc gaaaaagagg agtttcgaac catcttcctc
    66781 gatacgagaa atcgtgtgat tgaaagtgcg accatatcca taggcacgct gaactcttcc
    66841 atagttcatc cgcgcgaggt tttccgagca gctattcgcg caggcagtgc aggtgtgatc
    66901 ctggcccata atcatccaag cggtgatcct gccccgagcc ctgaggatat tgcaattaca
    66961 aagaggttgg tgcgcgctgg cgctttgatc gggatagaag tgctggatca cgtgataata
    67021 ggggacaatc tgtacatcag tttcaaggaa aaaggataca tgaatttcgg ctaattaaga
    67081 atcttgccac agaagggagc cattcgttat ggcgtgcgct tcgctctttg gcggcctggc
    67141 aagggacatg ggcattgacc ttggcaccgc taacacactt gtatatgtaa gaagcaaagg
    67201 catagtgctt caggaacctt ccgtgatcgc catcgaccgc gactctcagg agatcctggc
    67261 cgtgggttat gacgctaaga agatggtggg aagaacgcct gcaagcattg ttgcggtgag
    67321 gccgatgagg gacggcgtaa tagccgatct cgacgtgact gtcactatgt tgaggcactt
    67381 tattgaaaag gcctcaaagc ggcacggggt cttcaagcct cgggtggtaa taggggttcc
    67441 atcaggtgtt acgcaggttg aacgccgagt gatacatgac tcagcaatac aggcgggagc
    67501 gaaggaagca ttccttatcg aagagccgat ggctgcagct atcggcgccg ggctccctgt
    67561 ggaagacgcc accggcaaca tggttgtgga tatcggcggc ggaaccacgg aggttgcagt
    67621 tatatcccta ggcggcattg tggcggcaaa gtccattaca actgctggag acaagatgga
    67681 tgaagccatc gtgaactaca tgaaacgcaa ctacaatctg atgatagggg aacggactgc
    67741 agaggagata aagaagactg tggggtcggc ctacccttcg cagcaggaag agactatgga
    67801 agcgcgcgga cgcgacatcg tcaatgggct tcccaagact gtaaccattt ccaacacaga
    67861 gatacagcaa gcgctcgccg atccggtggg tgagattgtg aatgccgtgc gcatgacttt
    67921 ggagaaaact ccccctgaac tgtcggcgga cgtgatcgat aagggcatcg tcatgacagg
    67981 cggcggatct atgctgcagg ggttggatag gctgctctgt gacgtcacgg gcatgcccgt
    68041 ttaccttact gaggatccgc tgacatgtgt cgcgcgggga gcaggcaacg tcgtggagca
    68101 ccttacaacc tatagaaggg tccttgtatc gtcagggaga ctcaagtaag ggcccagagg
    68161 gggcgctccc atgaaccagc aacctttgtc aaaaagaaga atcgttgttg cggcaatctg
    68221 catcatcctc ttggtgtgct tgatatactt cactgcaaag gaccgcgaca ggctctctgt
    68281 tgcggagaag gcgttgcgcg aaatattctc ccctatagct cgaggggcaa gtaaggtgag
    68341 cagggagatc tcatcatggg cttcgtatct tatttcgata ggcgagacga aggccgagaa
    68401 tgagaagctt cgggaggagc ttgacgcgtt gcgctacgag aacttgcaga tggaggaggc
    68461 cttgcgagag aacgaaaggc tcagggactt gctggagttc aaggagagtc tagggggacg
    68521 cgccgtcgcc gccaggataa gctatcgtga cccaggaaac tggctttact ctgtggcaat
    68581 cgacaagggt tcagaccatg gaatttcaag cgggaaaccc gtagtcactg ctcgcggggt
    68641 tgttggaaga gtcacgtctg tcacccggaa tacgtctaca gtgattctga ctgtggattc
    68701 ccggagtgct gttggtggca tcgatttcag atcacgtgac ctcgtgctgg ttgaggggtt
    68761 gggcgacggt tcaggtttgc tgcacgttcg tcctttgggc gcagacaatg atgtgcagcc
    68821 aggggacata atcatcacct ctggccttgg tggcatatat cctaaaggct acgtgctcgg
    68881 tgatgtaatc tcggtggcgc ccgggaaata tggtgtgtcg aaatcggcgt ttgcgcggcc
    68941 tcgagtcgac tttgacaggc tcgaagaggt cttggtcttg atcgatccga taaggggagc
    69001 tattggggag atgcaggaat gaaaaggttg aacagactgc atattgcttt aggcatgttc
    69061 gttgcattgc tgttgcaggc tgcggtcttt cctgtgcttt tccccaggtg ggtagtgccc
    69121 gacctggtgt tttgtttttc ggcagtaatg tccatcatag cgggaactgg gcctggcgtc
    69181 gttctcgcag ctatcggcgg actatgccaa gatgcgctca gcggatcgtt catgggcgca
    69241 agcgctggtg cgaacatggc cgcggcatgg attatcggct tggttgagcc ccagcttttt
    69301 aaggagaatt tgattacgcc agtggtcatc gtgggcgcgg gaacggtgat ccgtgaggtg
    69361 atttacacat tcgttatctg gtcatttggc gcaggcatcg attgggccag agcgtttgga
    69421 acggtgattc cgtgggttgc ggtgctcaat tgttgcgcgg gcgccctgct ccactactgg
    69481 atctaccttc gtccacggga ccgagagcgt ttacgatcgg tttcgtctgt ctagactttt
    69541 ggcgtgaagc ttttccgggg ttgatgtatg atgaatcgaa cacgaacgca gagtgagcgc
    69601 cgactccggg tattccagat ctttgcctta tgtctgttcc tggtcctcgt gggcaggctg
    69661 tggcaactgc agataataaa gggagaatat tacaagagca gagctacggc taataggctc
    69721 gcacttgtgc cgatatctgc gccgcgaggg ataatcactg ataggcgcgg ggaagttttc
    69781 gccaccagca ggatggcata taccatctcc gttgttccgc aagagttttc ggatcgagag
    69841 gcggaggtgc ggcttctctc taaacttttg gaaatgcctg ttgaggagat cgaagcgaag
    69901 atagccgggc agactgaggg ccggtatggt ctgccttata tgcctacgcg cctcatcgag
    69961 gatgtgcccc cctacatgct tgtaagggtc tccgagcaca aagttgagtt gcccggggtt
    70021 ataatcgagg aagaaccgta tcggagctat cccaatggat ccttggccgg gcccatcatc
    70081 gggtacgtcg gtctgatcaa tcaggaagaa cttgctcgtc ttggcgaaaa gggctatcag
    70141 tcccgtgacc gtattggcaa gagtggtgtg gagcgggagt ttgaggaata cctacgtggg
    70201 caagatggag tcactgaaat cgaggttgac tcgctttctc gacctgttgc gacggtaggc
    70261 gccagggctc cggtggccgg ctctaccctg gtgatgacga tcgatgccaa agtgcaacag
    70321 tcggctgagg atgctctacg gtcgcagctt gagacgttgc agtctggcaa atacaaacac
    70381 gctacggcgg gcgccgcggt agcgctggat ccgcgcacag gagagataat agccatggca
    70441 actgagccag gctacgaccc gggctggttt gttcctagaa tctccgatga acagtggcgt
    70501 caggtgagca ctggcgtgtc ggggcttttc aaccgtgcga tatcaggcgc ttatccgccc
    70561 ggctccacgt ttaagccttt cactgccata gctgcgctcg aggcaggggt gatagatctc
    70621 gacgacagga ttctctgctc cccctctgtc tcctcgcgct actacaggaa gaggtgctct
    70681 atttggtcaa ggggccgttc gcacgggtat cagaatctta ctgagggtat gatcaattcc
    70741 tgcaacattg tcttctacga gctgggagca agactctcgg tggatcagat ggcttcggct
    70801 gcaaagcaat tcgggctttc ccagccgact ggcttgcgat ttacgcctgt ggaggctacc
    70861 ggagctgttc cagattccag tgttcgcgat ttcatgccgg gcgaacttgc aagctacgcc
    70921 attggccagc aggtgactgt tactccgctg cagatggctg tcgcctatgg tggaattgct
    70981 actcgaggcg tgatgtacaa gcctcatctt atcaggcaag tgatagcgcc tgatgggaca
    71041 gtacagacgc agtctgttcc tgaggtaatg cggacggctg tcctttctga cagaacgtgg
    71101 gactatcttc atcacagcct cagcgaagtg acgcgaatag gcacggctag ttcggctttt
    71161 agaaattttg gcatacccgt tgcaggcaag actgggactg cggaggttcc cccgggagat
    71221 ccacacgcat ggtttgtcgg gtgggcgcca gccgatgatc cggagatagt tgttgctgtt
    71281 atgatagagc gtggcggagg tggcggggcg tctgcagcgc ctgttgccag aaagatcatg
    71341 gaagcctatt tcgccccgac gggcgagtag gcaggaagga aatgcattcc tattggagaa
    71401 tcaaacatgg ataggctgaa tcggcggtgg ttgcggtgaa ggctcaggaa gtgattttta
    71461 agggaacaag ggacgggctt cgcctcgtta tagacgcaga tgagaatata gaagaactca
    71521 agggcagact tcgccgaagg cttaacgaag ctgagggttt ttactcaggt gccagggtcc
    71581 ggttggatac gcgcggggct gttgtcccgc ctgagtggat cgaggaggtc aggatgatct
    71641 tggaggagta cgggttgtcc ctgagtgagg agaagcctgg gcaacgaagg ccgcggcaac
    71701 atgccgccgg cgcgggtcgg gcttcggcta agaaggttct cgatgacggt ggcccagaga
    71761 gcggagaacc gatcgtgcgc ggcgacacga ttctcgtccg aagaactctc agatcagggc
    71821 aggcagtgtc gttcgatggg aatgtcgtcg tgatgggcga tgtcaatccg ggctccgaag
    71881 tcttggcaac tggcgacata gtggttttcg ggtcgttgcg agggatggcg catgcgggct
    71941 cggccggaaa cgccgaagcc tgtgtagttg ctctgcgttt gatcccgact cagctccgga
    72001 tagctgacaa aataacgagg gcgcccgatg acgaaacgca agtgccgcag ggtccggaag
    72061 tagcttatct gcgcgagggg tcgatcgtga tagaaccctg gacaactcat acggggacgg
    72121 tgttagtttg atgagcggga ctgctatagt agtgacttcc gggaaaggcg gtgttggcaa
    72181 gaccacagca gcggccaaca tcggcgctgg cctggcgatg tcggggccca ctgtagcact
    72241 ggtcgatgcg gatatcgggc ttcgcaatct cgatctcgtg ctagggctcg aaaatagaat
    72301 agtatacgac ctagtcaatg tagttgaggg tggttgcgat gttcagaaag cgctaatacg
    72361 tcataaacat attgaaaatc tctctcttct gccggctgcg cagtcaagag acaaaacagc
    72421 agtcacgcct gaacagatga aggctctcac cttggggctt aaggagagtt tcgattttgt
    72481 gatattggat tgtccggcag gtatcgagca ggggttcaaa aatgcgattg ccggggctga
    72541 ccgcgcgatc gtggtgacta ccccggagat gtctgcgtcc ctcaccgcag acatctccgg
    72601 ggtagttacc ccggagatgt ctgcggtgag ggacgcagac agaattatcg gaatgttgga
    72661 agcggaagag gttaaggacc catggctcat tgtcaacagg atccgccccg atatggtcaa
    72721 aagacacgac atgatgaacg ttgacgacct catagatttc ctcaaggttg aactcttggg
    72781 cgttgttccg gatgacgaat cgatcataat ctctaccaac agggggattc cggcggtaat
    72841 ggatgagaag tcccgtgctg gacgagcttt tctcgatatt gcatctcgga taatgggcaa
    72901 tgatgtgccg attgcggcga cgctcgaacc cgatgattgg ttttctcgat tcgccaagtg
    72961 gctagggata tcaagggggt aggcgccatg atggactttc tcgcgcggct gttcggaagg
    73021 gtggaaaccg agacaagcag ggaatgtgct aagaataggc tgaggcttgt actggtgcag
    73081 gatcgcgcta ccctctcacc gcgcgttatg gagcagctca aaagcgaact gattgaagtt
    73141 attagcaagt atttggacat agatgaagag gacatgcgcg tcgaactaga cacgtctgat
    73201 tcatccgttg ccttggtggc gagcattcca gtcaaagagg ttaagcgcga gtcggttgga
    73261 gcggtctagc ttgactttgc attatggccc cttactggac caggtttgtt attcgtgctc
    73321 tttcaggagg ccgtagtatg ctcattgata ggaggcttct tagcaatctc gatcatgcat
    73381 tgcttgttgc agtagtcatt gcagttctat ttggttgtgc catgatatac agcgcaacaa
    73441 gatggaatac gcgcttgacg gcgggaaacc cgcttcttta tgtgaagaag caattggctg
    73501 ctgcggcgtt gggggctgct gcgtcattgg tcgtcctcag tgtggactat cactacttgg
    73561 aacggtttgc caagtggatc tacctggcta gtctattgct gcttgcaagt gtttttgttt
    73621 ttgggaagcg gactaagggt gcgataggtt ggattaagat aggacccgtg gccatacagc
    73681 catgtgaata cgccaagata ggggtggtta tagcccttgc tgcgtacctt gctcagcagg
    73741 atgatctttc cgatttgcgg agtttcattg tgccagctct gatcgtggcg gcgccgatgg
    73801 cgcttgtggt tgcgcaaaac gacttgggtt ctgcactcgt tcttgccccc gtgtttttcg
    73861 caatgatgta tgcggctgga gctgatgcga aactgttgtt ggcgattgtc ggggttattc
    73921 ttctggtgat agctccgttt tcctattttt ttctgctgca aactcatcaa cagatgagga
    73981 tcaaggtatt cttcaatcct gggcttgatc cgcgcggata tggatacaat gtgatgcagt
    74041 cagtgatagc gatcggatcg ggtgggctat taggaaaggg gtatggccag agcactcagg
    74101 ctcggctcaa tttcctgccg gagcaccaca ccgactttat attctccgtg atcgccgagg
    74161 aattagggtt tttcggggcg actgcggcgc tggcagtcct ctttttcata gtgcacagag
    74221 gttatagcgt ggcccaggag gcgcgcgaca agttcggcgc tcttctggcc gttgggctaa
    74281 cctcggttat gctagttcat attttcgtaa atgcgggaat gactatgaat gtgtcgccgt
    74341 gcactggaat accgttgcca tttttcagcg caggcgggag tgcattgatg gccatgacga
    74401 tttcaatcgc catagtgcag aacgtataca tgaggaggaa gaagatcatc ttctagcgtg
    74461 gctgagacgg caatataatc tcatatgggc attcgaggct gcaggctctg ggggcttgcc
    74521 gctttctctg ctctgatctg gggcactggg ggtagggcag gtgaagcgcg cgctgccgcg
    74581 ggaggttccg gggattctgg gaatgacacg tgtgttcgga atcgaattcg atgtagaccc
    74641 gctcgttgtc gtctttgcag ctttatacgc gttcgcaggg aatctgtggc aatggatggt
    74701 cgtatttgcg tcgttgcttg ttcatgaggc agcgcacgct gtcaccgcgg caggctttgg
    74761 attcgcagtg gcgcgaatca gcgtaacacc gatcggggcc gtcgcctcgc ttgagggaga
    74821 tattcagctc cgaccagagg ccgaagcggc tgtagccatg tccgggccca tgacttctct
    74881 agttcttgct gccatgggtt atcttctcct gatttatggt aatgctgata gggggaagat
    74941 cgagtttttt gtgggcgcca acatcgtttt ggccatgttc aacctgattc cggcatttcc
    75001 actcgatggc ggacgggttc tgagagccct gatgactgag aggttggggg ttgcgtctgc
    75061 cacgcgcaag gcggttggca tgtcgcgtgt tattgggatt ggaattgcag ttttgggcgt
    75121 ggcgggcgtt gccgcgggat attttaattt tttggtgccg gcgttgggac tgttcattct
    75181 gatagcagct gagcgtgaga ggcagtctgc agtatgtgct gggctcaaat ccgtgatggg
    75241 caagcgcgtt tcattgagca agcgaggcgc gttaccagtg agattgctgg tggcagctcc
    75301 cgagatgacc gcttgcgacc tgcttcgcaa agtgacgtca tccgacttca ctgttgtgtc
    75361 ggttattggt gcaggtggcc gcgagatcgg acgcctttcc gaactagagc tgctggaggg
    75421 catatcgatg ttggggcccg atgctgaggt tcgtgaagtc ctggccggcc gtaaagattg
    75481 aggagaagcg ttttttatga ttgacaagaa ggttttgtac gaggaaatcc tgccgtcggt
    75541 gtcgcagccg gcaaggtacg tcggcaacga gctaaactcg gtccgccgta gtccgaaaga
    75601 ggttgaggcc agtatcttgc tggcttttcc ggacgtttac gaggtgggaa tgtcgtatat
    75661 agggttcaag atcctgtacg aaatagtcaa caacataccc gaattcgtcg cggagcgcgc
    75721 ttttgctcca tggcctgata tggaggcgca aatgcgcttg cgcgaaattc ccctttacgg
    75781 cctcgaaacc tacgagccag cgaagtcctt cgacgtgatc gggtttaccc ttcagtatga
    75841 actgacatac accaatgtgc tggcaatgct cgagttggct ggtgtgccgc ttgaggctga
    75901 tgcccggcac gaaggcgacc cgatcgtgat tgcaggtggg ccatgtgcgt ccaatcccga
    75961 acccatggcg gcattttttg atttgcttgt gatcggcgat ggggaagagg cgattgtgga
    76021 cttgatgtcc gaggttgcca gggcaaaaag agagggggcg aaacgcgctg acatagtaaa
    76081 taggcttgcg gtgatagatg gtgtctattg cccgcgcctg aagaaccctg taagccgcag
    76141 ggtggtgacg gatcttgacg aggccgagtt tccaagaaag tttgtggtgc cttttctcga
    76201 agctgtacac gaccgggttg ctctggaagt gatgaggggt tgcacaagag ggtgcagatt
    76261 ttgccaagcc ggcatgatat accggccagt gcgggaacgc ggactcgaca atctctgctc
    76321 gctggcgaaa gatcttgtca aaagcacggg ctatgacgag atatctttgc tttcgctttc
    76381 aagcgcggat tacactcagg ttgaggagtt gagcagaaag ctcgttcagg aatacgcaga
    76441 caagggagta gcggtttccc tgccttcttt gcgtgtggat tcgttttctt tggagcttgc
    76501 caaggagctg gagaagtcgc gaaggacggg cctgactttt gcgcctgagg ccggaaccca
    76561 aaggatgcgg gatgtgatca acaagggcgt taccgaagag gatctgatgt ctgccgctgc
    76621 agacgccttt gaatcgggat gggatctcat caagctttat ttcatgatag ggctgccgta
    76681 tgaggaggac gaggatctgg atggcatagc agatctctct cgtcgtgttt tggagcttgg
    76741 acggaatatg gcggggaagc gaaacaaggc gggcagggtc cgagtaaacg tgagcgtatc
    76801 gtcgtttgtt cctaaaccac ataccccttt tcagtggcac gcgcagaatt cccgggagga
    76861 attgatgaga aagcagaatt acctgaagga acgaatgcgc gtgcgcggct tgacagtctc
    76921 gtttcacaat gtggaggcaa gcttcctgga agcggcgttt gcgagagggg gcagagagct
    76981 tgctccggcg atagcgcgcg cggcggcgct cggatgcagg tttgacggat ggtcagacca
    77041 gttccgccct gacctatggg cgcaagcttt taaagatgtt ggcattgacc cttccgcatg
    77101 ggccaataaa tcctatgcat atgatgagcc gctgccatgg gatcacatcg acatgggagt
    77161 ctcgaaaaga tttctcgtgc gggaagcaga acgtgccgcc agggctgaac ttacccccga
    77221 ttgccgcact ggtgcttgcg cagcctgtgg cgcctgcgcg gatggcgtca agacgcgtct
    77281 tgccggcgag gtatggaggt gaacggggta tggtgctgag ggttcgcatt acaaaaggcg
    77341 aagaggcgcg gttcatctca caccttggtt tcatgagagc ttttgagcgc gccataaggc
    77401 gtgctgagat tccggttgca tattctgaag gctaccaccc tttgcccaag ttctcatttg
    77461 ccaccgctct tggggtcgga atttccagcg aagcggaata tgttgacatt cagatagatc
    77521 ctgacggtcc caagatcagc gttgaggagg ccttcgacgc gctggacagg aacatgcctc
    77581 cggggattgc ggtcgtggcg gcctgctggt atctgggtaa acctgcactc gcatcactta
    77641 ttgaatacgc ttcctatagg gcgagtggat gtggcgcctt gcatgaagcc gcagaagagt
    77701 ttatggccat ggatgcctcc ctagggacta tcaccacgaa gagcggggaa aaagaggagg
    77761 atatcaggaa gctcgtttat tcaatatcga tgacagatga tgagagtttg gagtttattt
    77821 gtgcgtgtgg cgggcgcgct catctcaggc ctgataacct tcttgtgcac ctaagttcga
    77881 tcgccgggat tgaatgtgat gtcaagcggt cccggatcca tcggactggt ttgtttgttg
    77941 ttgaggatgg ggaactcatg tcccctatgg attataaatg caaagatttg gtcatcgcta
    78001 agggttggat gcccagggag ttgagttgaa tgtccagaga aattgttgtc aactccgaca
    78061 atggcgaaac aagggccgcc atcctggagg atggaaagct tgtggatctt tttgtggaga
    78121 gatcagttca tccccggtat gcggggaacg tctacaaagg agtagtcgag aatgttcttc
    78181 cggggatgca ggcggctttc gtcaatatag gtttggagcg caacgcgttt ttgtatgtag
    78241 atgatgcctt agcagggcat aatggccgca attcccgcgt agtacgggag gcggatggag
    78301 aagagatttc ggttccgagg aagaagtcgc gatcgattaa ggacatctta aagcctggtc
    78361 aggagatcat ggttcaggtt acgaaagaac ccataggcgc caagggcgca agggtagtga
    78421 cggatgtcac tcttccagga aggtatgttg tgctcatgcc cacggtggac tatgttggtg
    78481 tgtccagacg catagaagaa gagggcgagc gtgacagact gcggaaaatc gctcaggcct
    78541 cgaaaccgcg tcgaatgggc gtgattgtga ggacggttgc cgaagggaag agccaggagg
    78601 agatcgcctc cgatattcag tttcttgtga aattatggag gcgaatacag ggcaggaaca
    78661 ggagggcgca gggcgccacg ttgcttcacc aagagtacga cttggctttc aggttggttc
    78721 gcgatcattt tgctgcggat gtaaccaggt ttgtggtgga tgatgctaaa gagcataaga
    78781 aagtcctcga tctgtcgagg atgttttccg agtccatgcg ggaccgaatt cacctgtata
    78841 cggggcagga accgatcttc gatgtgtatg ggctcgagga agagatagcg cggaccttga
    78901 ggcgcaaggt gtggcttgca tgtggaggat atattgtcat agacaacaca gaggcgctca
    78961 cggtcataga tgtgaatacg ggaaaataca ttggcacgac atgtcttgcg gacacggtac
    79021 ttaagactaa catagaagct gctgaggaaa tcgcaaggca ggtcaggttg agaaacattg
    79081 gcgggataat agtcatcgac ttcattgata tggatgatga ttcccatcgg cgactggtca
    79141 tagagaaact cgaggacgct ctctcgcgcg acaaaacaaa ggccacagtt cttggcttta
    79201 ctcacctagg cttggtggag atgactagaa aaaaggtgca gcaaggattg gctgagtcga
    79261 tgatgaaagt ctgtcctacg tgcgacggga gagggcggat cctctccgaa gagacacttt
    79321 catttcgcgc tatgcgtgct attaagaagg aagccctcac gactgatcag ccagctatgc
    79381 ttgttttgct ccacccgtcg gttgccgcca tgctcatagg cgctggtggc tctaaccttt
    79441 ctgcgctaga agaggaaaca ggaaaagcca tatacgtgaa gggcagcttc gatcagaagg
    79501 tggaggacat agtagttgcc gccgtcggat cgagggaaga cgtagagaag aaggcattgc
    79561 ctgtgtcagc cggcgatagg ttggaagtgg tcattgaaga acctcatgtg agcaatagtc
    79621 gagacggaat tgccaggctt gaggggtatg tcatagatgt ggagggcgca ggtcgccttg
    79681 tcggcgagaa agttcgcata gaggtcacaa aggttttcaa gacgtatgcg agggcaagat
    79741 tgatcaatga agacgccgtc gagcatgggc aggataagga aggccagaat gcggtagtgg
    79801 ctgagaacgg cgacaagcgc gaggataagg agaaatcggc caagaaggct tctaatgaag
    79861 atcacgtgaa agacgagcaa aaagccaggc aaaacagtaa gaaagacagc aaacaagatg
    79921 ccaggaagag taacaaacaa gacagcaagc aagatagcaa acagaaaagt ggtcaaagcg
    79981 atggcagccc gaagggcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    80041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    80101 ggagtgcgtg cgaaggatga tccagaggca agttttggca gtacgcggat gccgaaaaca
    80161 agtagcacat gttccttgaa ggatatccca acgcaaatgt gtgcagttcg gagctggacg
    80221 tgtggttcgc cgcttcattt ctggaaatgc ctttgactga caacagaaac cgtctactat
    80281 gggttgggga aattatccgc gaggctttgt tcgggcaata cagattctac gatagagaac
    80341 ttcgcggcgg ctaaaccaaa gagggctacg acaagcaatt tgagaatgcg acttcttcga
    80401 gtaacccgac gagcgcaagg gttacggcgg agggaattac gtgggccacg gcgatcagca
    80461 tctatggcca gctgaagacg gtacaggcct gcggggtgcg gggcttatat cttttctagc
    80521 aagcagcgca ggggcaagtg ctattggatg aggtgtgctg tttgacagcc gcagcagcat
    80581 atgctatatt ttaaccgctg tgggcgactc tgcgtgtcgc ctcaagccgc tcggggaagg
    80641 cttccaaatg gagtgatcgc caacttggtg gcacgcctgc cttctgtttt tctcttttat
    80701 cccggcgagc ccagagtgcg aggaggtaaa aggatagtgt atgcaattat cgaaaccggc
    80761 ggaaaacagt accgtgttga gccggggcag gtcgtccgtg tcgagaagct ggacgctgag
    80821 gtcggcgaca gtatcgaatt cgaccgtgtg cttatggtgt caggcgacga tggaatcaag
    80881 gtaggtagtc cagttgttga cggtgcaagg gtcaagggcg ccattcttga gcatggtaag
    80941 gccaagaaga tctatgtttt caaatacaag gctaaaaaac gagtccgcac gaaaacagga
    81001 cataggcagc cgtttacagc ggtaaagatc gaatcgatct cggtggcgta gtttgctctg
    81061 atgagcgcgg tgcaagaagg tttttgccgt ggttaagctt catgttcggc gtgtggaggg
    81121 ccatgtggtt cggtttgccc tgcatgggca tgcggaatgc ggagaatatg gctatgattt
    81181 ggtatgtgct gctacgtcag ctttgacgat tgccgctgca aatgggcttt ttgagcatgc
    81241 aggtgtgtct cgtgccgtaa ggtatcgttc cggatacctg gattgcgtga ttttgaattg
    81301 cccatctgcg gaggtgcgta tgcgagccga tgccgttttg gagaccatga tttctgggat
    81361 tcgagccata gcgctagagt atccagaaag tgtttccgtc gttgacatcg gaggttcggt
    81421 ctcggcgcga tattgaggag aaagatgcgc cttggcgtnn nnnnnnnnnn nnnnnnnnnn
    81481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    81541 nnnnnnnnnn nnnnnnnngg cgtgtgagtt aaggcgaaat gaaacggaat ccgggaggtg
    81601 tttccaatga tcagaatgga ccttcagcta tttgcgaaga agaagggagt cggaagctcc
    81661 cgaaacggtc gtgactcccg ttcgcagagg ctcggagtta agaagtttgg cggccaatac
    81721 gtttccgccg gcaacatcat tgtcaggcag tgtgggacta aggttcaccc tggtatcaat
    81781 gtggggatgg gtaaggacta taccctgttt gctctggcag acggttatgt tcagttcgag
    81841 cacaacggaa agcgaagcaa gaaggtttca gtcgttcctg agctgcctgt tgccgaggag
    81901 ccagcgcatg tcgttgaatc tatgtagttg gtgattggag gctgaagggc tcgcatcagg
    81961 tgtgcttgat gtgggccctt ttgttatcgc tgtgctactc cttctgagag ggtgaggttg
    82021 atgggcaaga taccagtctg ggctagagtc ttgatagtgg ccattattat cgtggcagtc
    82081 gggtggaaga cgatttctaa tataacgact gagtatctgt ggtaccagga cttggggtat
    82141 gtgagtgtat tctggaagtg cctggaagcg agggttgctg tcgcagcagc gatatttgcc
    82201 gtgacatttg tctttgttgc tgcaaatctt ctgtcaatga aatcggcgtc gaaagctatg
    82261 ccaagacgat cggcactttt cctggctaca attgcgggag tgttgactgc cgggttttct
    82321 acagatttgt ggatggaatt ccagcagttt gtgcatgcta cgccgtttgg gaaagcagac
    82381 ccgattttcg gccaagatgt aggcttctac gttttccgat atcccttcct taaagaagtc
    82441 tacaaaggag caatatgggt tgtgggcttg agcgctacct ttgtcattct tgtctacctt
    82501 ttttccggat cgctgatcag atttagccga gaagaagtgg aagaacccgc aataggcagc
    82561 cgccgcagga ggcccaggcg aggaaggctt ctcttggaac aggctgatcc cggcacgaag
    82621 cttcacatag gcatacttct tgccatcgtt ttttgtgttg tggccttcgg ttattggctt
    82681 gacatgcggg gattggtcta ttccgagagg ggagttggca taggcgcaag ctatgctgat
    82741 gtacatggcg ctctaccagg catgcggctt atgctcttca tgtccctcgc agctgcagta
    82801 atcatgataa tctctgccat atcacagact cgtgtaaaga cattggcatg gatggctgct
    82861 acggtagtga tcatgggagt cttttcgcta ctggctgtaa atatctatcc caatctagtc
    82921 cagcgcctct atgtgacgcc caacgagttg agggcagaaa gggagtacat ctcgcacaac
    82981 atcgacttca ccagaagtgc ttttggtctc gacagtatcg aagagaagga atacgttgtg
    83041 gatcagaacc tctcctggac cgacgttaac tcggcacggg ccactctcga cagcataagg
    83101 ctttgggact gggagccatt gcttgcaact tacgggcaac ttcagggcat gcggctttac
    83161 taccagttta acgatgtgga cgttgatagg taccttattg acggcaacct tgtccaggtt
    83221 atgctggctg caagagaact cgacgtggaa agtctgccgc ctcaggcgca gacatgggtc
    83281 aacaggcgtc tcaagtacac gcatggctac ggcgtgtgtg cgagtcctgc gaatgagata
    83341 ggccctgacg gacttcccac attcctattg cgagatatac cgcctaaagg ttctgatagc
    83401 ctcgagattg agcggcctga gatatacttc ggtgaactta ctcgcgattg ggttgtcgtc
    83461 aatacgaaga ctgaagagtt tgactatccg gtaggtgata ccaataagtc tacccggcac
    83521 atgggtgact ccggtgtccg catttccaat cctctgcggc gcgcgatgtt cgccacgaag
    83581 ttttccgacg ctaggctgct gttgtcgtct gatatcactt ctgacagcag gatattattt
    83641 gatagggaga ttgggcagcg tgctagaaaa atagtcccat tcctcagcta tgacagtgat
    83701 ccatacatcg tagttgctga cggtaggctg atatggattt tagatggcct taccacgtca
    83761 gaccgatatc ctttttccga aagatatgtg ggaggcatca actacatcag gaattccgtg
    83821 aaggtcacga ttgatgccta taccggccag acggatttct atctcatcga cagaaatgac
    83881 ccggtggcgg ttacgtacgc aaagatattc ccgggactgt ttaaggaagc cgatgaaatg
    83941 ccgcaaagca tacgtgaaca cgtgcgttat ccagaagacc tcttcacgat tcaggccaag
    84001 atgtacggga tgtaccatat gagggatccc gaggtgttct acaacaagga agacctctgg
    84061 gcaattccaa atgagcttta cggtggttct gaacgcgaag tgagccctta ctatatccga
    84121 ttggtgcttc caggtgagtc cgatatgggc ttcgtgcttt tcgtgccgtt cacgccttca
    84181 cggaaggaca acatggttgc atggctcgcg gtaaacagcg atatggatga tttcggccgg
    84241 atggtggcgt acaagttcgg aaagcaaaag gtcatatttg gcccaatgca gatagaggcc
    84301 cagatagacc aggacgcaga gatctcaaag atgctgtcgc tgtggaatca gagcgggtca
    84361 actgttattc ggggcaacct gttggtgtat cctattggcg gcggattgct gtacgtggaa
    84421 ccgctatttc tggcagctca gacaagccag ctgccccagt tgaagcgcgt ggtgctatgc
    84481 gacggagcca aggtcgccat aggaagcgat ctgtggagcg ctcttgacgc gatgctttcc
    84541 gggcatggcg aaggagcgac aggtagtttt ctcgattcgg atgagcttgg caatacaagg
    84601 gaactggctc atcaggccct caaactctac cagcaggctc gcgatcgcct caaagatggc
    84661 gacctggcag gttatgacag catacagcgg gagctagagg acttgcttga gtctatggca
    84721 accgaagaat aggcagggag cgagggactg tggtaggctt ggcggaattg gatttaaaga
    84781 ctaactggaa agatggcatc tgtgaaataa ctgggtttga cagcttcaac ttgcattctt
    84841 cgtgccattg tggccaagca tttcgttggc gcacatctgg atcgagaggg cagttggaag
    84901 aaggcgtggt tcgtgggaag ttgcttcgtc ttgaacaacg cgatgatgcg ctcataatcc
    84961 atccgccagt cgatgatgag acggtcagaa ttgccatcga ctattttcgc ctcgccgttc
    85021 cgcatagtcc gatagaagag gctatagctg ccacagacga gatcatgttc gaggcggttg
    85081 agaggaatcg gggtcttcga atcgttgctc aagagccttg ggaatgcctg gtgtcctaca
    85141 ttatatcggc aaggaatagc atacctcaga tctcgcgtac agtggagagg atagcggaag
    85201 catggggtgc gcccgtattt gacgttccga cttcgcatga tggcgggagc cgagatggat
    85261 tagtggatgc ggccggctat gaggccagtg ggaagaaggt gtggtacgcc ttccccggtc
    85321 cgcgcgagat gtgctgcgcc acagtggatg agctggcggc atgcaagact ggtttcaggg
    85381 cgcggtcagt ctgcgaagcg gccaacagag tagcgtcggg cgacatcgat cttgacggaa
    85441 tcgcatcgtt ggactatcct cttgccaagg aagagttgat ccgactgcgc ggtgtgggcg
    85501 agaaggttgc agactgtgtt ctactctttt ctatgggaaa gtatgaagcg ttcccggtgg
    85561 atgtatggat cgaacgagca gttaggcacc tgtatttcga cggtgaggaa atcagccacc
    85621 gtgagattcg caagtgggct gcaggcaagt tcggagaggt tgccggacct gcccaagaat
    85681 atctgtttgc ctatgcccgt gaggcgatac ctgagaaact caagaagggg taggcggagt
    85741 ggctgaccgg atagatagtc gaaaaagtga ttttcccccc ttgcatgcct cgaggtcgta
    85801 cgttataata attaagcgcc ggg
//
LOCUS       DAOSYL010000005        62139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_378_length_62139_cov_8.656723, whole genome shotgun sequence.
ACCESSION   DAOSYL010000005 DAOSYL010000000
VERSION     DAOSYL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 62139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62139
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_378_length_62139_cov_8.656723"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1611)
                     /locus_tag="PLU15_02105"
     CDS             complement(265..1611)
                     /locus_tag="PLU15_02105"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO29745.1"
                     /translation="MKELELCDSLVERARKMGADDAEVFYIKSRQLGAVFEKNDLQVP
                     KGDDYDGLGIRVLLYGKGGAKQGFAATNMLSPDSLEETLLAAIAIANASPDDPNHWMP
                     EPSTIEPVPGRYDESAETMSLEDAVEQGLALVNSARAFDPRVTLDSAQYMVDITEKAI
                     VNSKGIRLVEKDSVFTCLAMGFARDEKEVSSFDLEVAVTCRKDEIDPTAIGRKLAEKV
                     VASLGATTVPSFKGDVIITPYAGYDLIAEPISFSCNAQNVQNGRSKWAGMLGTEVMSP
                     VISVTDDPKLPKAAGSTSFDREGVPPAALQLVSEGTLNHYLYNCYTAKTDGVASNGRA
                     AGSDHTVPGISTTNFVVASGKHSLDEMIAGCSKGLLVSRFSGNVDPVSGDFSGLVKGG
                     HYIEAGKIVCPVKEVMISGNIYDLLRQVVDVSREYVDVGGSRMPYIQIEGCSITGK"
     gene            complement(1628..3004)
                     /locus_tag="PLU15_02110"
     CDS             complement(1628..3004)
                     /locus_tag="PLU15_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO29746.1"
                     /translation="MLDLLKSVISGCNDYAEIRYHKRIQDIIEARNGALTKVEHRTLA
                     GAGIRVLADGGWGFVSTNDVTESGLKKALSEAQAAARAGAAVRKDKAQLAAVEPAQGE
                     LVYYDATEEPDIAEKIRALLEAEKRLRERSPLIVGSITRYTQYEDEKYIVTSDGSAVH
                     VLDNKPSFHVFATAGRDGQLETAIKSAGVTGSWADLMRKGDLNTLVDEAVDLAVKKLD
                     AEYAKGGQYIVILDPGLVGVLSHEAIGHTVEADFVMSGSVANGKIGKEVASELVTMVD
                     DGTVAGAAGMVLVDDEGVAGKRTVIIDHGILRDYLHSRESAAAFGMEPKGNARAFTYR
                     DEPIIRMTNTFILPGEDNLDEMIAGIDDGFFLKELGQGGQADATAEFMFGVAEAYRIV
                     DGKVGEMVKGITISGQAFDVLESVDAVSSDFEMAMGAGHCGKFQAAKVDAGGPYLRCR
                     VTVGGRHE"
     gene            3723..4199
                     /locus_tag="PLU15_02115"
     CDS             3723..4199
                     /locus_tag="PLU15_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO29747.1"
                     /translation="MADKAEVLNDVLVKLFNKILSIEEEAITKAAALGVTMSEIHVIE
                     AIGTDEPKSMSEVASTLGVTMGTLTASVNRLVHKGHVSRFRPEEDRRVVLVTLTEDGR
                     LAHSIHERFHRRMINGILQDLDEESQDAIIVAAEKLYEFFERVKPEDLLLDNDSRQ"
     gene            4380..5369
                     /locus_tag="PLU15_02120"
     CDS             4380..5369
                     /locus_tag="PLU15_02120"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOO29748.1"
                     /translation="MYTKILGTGSALPKQRVTNEDLAMLVDTSNEWITSRTGISERWI
                     STGESASYLAAEAAAKALADADVEPSQIDLVISATVSPDNFTPTVSCEAQKALGLSRA
                     TCFDLWAGCTGFIFSLMTADALVKSGLFSHALVIGVEVLSKLVDWQDRSTCVLFGDGA
                     GAVVLSSSSQPGLVTHVSGSDGTRGDVLKIAGIPLANPWMAQHVGESETGSQSVICMN
                     GQEVFKFAVKTMESSIRRVVELSGSSLHDISYIIPHQANNRIIESVAGRMKIPREKFF
                     TNLDHRGNTSSASIPMALDEMNRAGLLLPGMHLVLVGFGGGLTWGASMVRWTR"
     gene            5454..5693
                     /gene="acpP"
                     /locus_tag="PLU15_02125"
     CDS             5454..5693
                     /gene="acpP"
                     /locus_tag="PLU15_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014658851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO29749.1"
                     /translation="MTLDELKAIISDQLEVSPEEISTGTRFVQDLGADSLDMVELIMS
                     IEEKLGVRIEQQEVKDMVTVGDVVSYIEAHKQAVK"
     gene            5730..6704
                     /locus_tag="PLU15_02130"
     CDS             5730..6704
                     /locus_tag="PLU15_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOO29750.1"
                     /translation="MPVRRLGEKLTRLFGIEYPVLQGGMAWVADARLAAAVSNAGGLG
                     IIASAHLSPDELREEIELARSFAGSHPLGVNIMLASPNAMDVARVCREKKVEVVTTGA
                     GHPGELIGEMKDAGILVIPLVASTAQAKRAVAAGADAVIAEGYEAGGHIGELTTMALT
                     PQVVDSVDVPVVAAGGIADGRGMAAAFCLGASGVQVGTAFLAARECTVHSNYKRKVLQ
                     AKDSDTQVTGRSLGHPVRVLKNRFSREFARMEREGADPDKITAYGMGRLAAAVRDGDV
                     EWGSVMAGQIAGLVTAERTAEEIVRGLVEDAARVLESLRVCQKGECCP"
     gene            6701..7774
                     /gene="fabD"
                     /locus_tag="PLU15_02135"
     CDS             6701..7774
                     /gene="fabD"
                     /locus_tag="PLU15_02135"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOO29751.1"
                     /translation="MKIAYVFPGQGAQYPGMAKEIYDQFASARQVFKTASRTLGYSME
                     RLCFEEGDRLNLTEYTQPALVTASCAVLAAMHESLNLSADTAAGLSLGEYSALVCAGA
                     VDFADAVMLVSKRGRFMQEACPAGAGTMATVLGLDRDCVIDICLDVQKAGAGIVEPAN
                     FNCPGQIVISGETAAVERAAELAQSRGARRVIRLNVSGPFHSSLLSAVGTRLRSELAQ
                     VDITSPTIPVYSNVAARPSMDPDEIRELLVRQVASPVLWEQTVANMAEAGIDVFIEVG
                     PGRTLTGLVKKTVPHAVAMNVEDLSSLEALREFLAEHASVHLPSQVAQVAAAALKTEP
                     ASLRKVSYATQPELVSSSKRAYR"
     gene            7771..8538
                     /gene="fabG"
                     /locus_tag="PLU15_02140"
     CDS             7771..8538
                     /gene="fabG"
                     /locus_tag="PLU15_02140"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOO29752.1"
                     /translation="MTAPRLDNRVALVTGGARGIGLAIAQRLAADGARVAINYCSSDP
                     QVVDKAVMSIESAAAGAGQKVRAYRADVSKHEEVGHMFEQAMSELGRLDIVVANSGIT
                     RDDLAVRMESQAFMDVIETNLLGAFLCAKEAARAMLRQRSGRIVLVSSIVGLDGNAGQ
                     ANYAASKAGLVGLAKSLARELGSRGITVNVVAPGFIDTDMTRALPEKMRDSCIARAAL
                     RRIGSPQEVADAVAFLASEEASYITGQVLRVDGGLVL"
     gene            8589..9827
                     /gene="fabF"
                     /locus_tag="PLU15_02145"
     CDS             8589..9827
                     /gene="fabF"
                     /locus_tag="PLU15_02145"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOO29753.1"
                     /translation="MRRRVVVTGMGAVTPVGCGWRPFWEAISSGQSGIRRIERFDPST
                     CPAKIAGEVREFDPDRYLDRKDRKRMDKFAQYAAVAASQAMEDAGFAAGGAPAPERLG
                     VAFSSGVGGMETFEEQHKRLIERGPEWVSPFFVPMMIANMAAGNIAIMFNAKGPSECQ
                     TTACAASSHSIADAYRLIAEGRADAMIAGGAEAAITPLAMAGFANMQALSRSEDPQAA
                     PCPFDRRRDGFVMGEGSGALILEELDSALARGARIYAEIIGCGFSTDAHHITSPPPGG
                     EGAARAMQEALHDASLAPGDIDYINAHGTGTPYNDLYETQAIKAVFGDDAYRLAVSST
                     KSMTGHLLGAAGAVEAIACVGAITEGFVPPTINYLEPDPECDLDYVANEGRKMEVRRA
                     MSNSFGFGGHNCCIVFTRWE"
     gene            9862..10362
                     /gene="fabZ"
                     /locus_tag="PLU15_02150"
     CDS             9862..10362
                     /gene="fabZ"
                     /locus_tag="PLU15_02150"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOO29754.1"
                     /translation="MGETGQLRSLDDALALLPHKDPFVFVDEIVYLEPGVKAVGIKRV
                     RPDEFWVPGHFPDRPTMPGVLIIEAMAQVGAVALLAKDVDGVRKNPKESAKGRRLLPL
                     FAGVDKVRFRRPVRPGDSLALETELLGTWGNFGRARSIAQLTDGSPVAEAELMFALGD
                     LDGGDL"
     gene            10749..11513
                     /locus_tag="PLU15_02155"
     CDS             10749..11513
                     /locus_tag="PLU15_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOO29755.1"
                     /translation="MHSVHSPDSDATMDEMCEAAIDAGLRGICFTEHVDLDPHYCSYG
                     SYDYAETMKDTARCREKYGDRLIIKSGVEITYLTEYADDVRQFLTEHQFDYVLGSIHI
                     IDHVFVGSEEHFIGKTEQEAYEPYWREMIAMMESGLIKRVGHMDYIKNHRPKEYGPFD
                     LKKWEDYIRHILHLVVENGATLEVNTSAIRRGFPEPYPSWDILELYREVGGTQVTIGS
                     DAHKPDDIGQYFADVAKRLDEMGFVVCGEEIFSCRV"
     gene            11627..12160
                     /locus_tag="PLU15_02160"
                     /pseudo
     CDS             11627..12160
                     /locus_tag="PLU15_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
     gene            12741..13910
                     /locus_tag="PLU15_02165"
     CDS             12741..13910
                     /locus_tag="PLU15_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO29756.1"
                     /translation="MSTSEYGFDYADLRESMVEEFCELVRIDAESGQEAELASVVAAK
                     LENLGFDVTQDDAGSKFGGNAGNVIGRFPGVPGLEPILLSAHLDRVTPGKGIVPSIDG
                     DRIVSGGDTVLAADDLGGVVAILAGIRMARAACKRLPPLEVVFTVSEEKGLRGARCLD
                     YSQIQSKTGYIFDASSPVGVVVVQSPTHISVDATITGRAAHAAVNPEGGISAIRVAAE
                     AIGKFPFGRVDDRTTANIGIISGGTATNIVCEKVSFKGEIRSLDTQRALDLAKEVAEQ
                     IKTTAGGYEASAEVTTTVDYYGYTIPRDAQVVRRVEKALAMSGREAVLDSTMGGSDGN
                     IFNTKGIESVVIGMGAMDVHSTKESLPISELVAAGELARNIILLASGMQDASLAD"
     assembly_gap    14407..14506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15036..16022
                     /locus_tag="PLU15_02170"
     CDS             15036..16022
                     /locus_tag="PLU15_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO29757.1"
                     /translation="MFKARLLCPGLLMLVVIALTIPMSFFEYVPWMEGDMASRVMEPV
                     LVCEEIIYKEIGEMSLGFDIYRSSEISASPHMAPVVVYVHGGAWVVGDKRSGLRLAWP
                     MLEALCSKGFVFVSTDYRLANTYTKFPAPVEDVRDTVRYLKKNADQLGIDSERIALMG
                     VSAGGHLALMAALAPDDSLGGDPKLCTYSGRVRAVVSWCGPTDLRIEQHRTPIREILV
                     AELVGQPFQNDSYLYSAASPAAYVSSASPPILLLHSEEDSLVPYGQAQCMYEQAMVVG
                     ADVMLISIENSGHVYKKIDDREIQPELEDILKLIADYIERQLGLAFGPCEHR"
     gene            16450..16755
                     /locus_tag="PLU15_02175"
     CDS             16450..16755
                     /locus_tag="PLU15_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOO29758.1"
                     /translation="MACEKQDRRSGQPEAKRVGVAAIIIQDRLNAAPAVNKILSEYGE
                     IIVGRMGIPYTDRHVSVIALIVDGDTDAIGAMTGKLGSIPGVKSRVTLVPSDNESGE"
     gene            16748..17959
                     /locus_tag="PLU15_02180"
     CDS             16748..17959
                     /locus_tag="PLU15_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO29759.1"
                     /translation="MNDADFTEVLGMAVSHPEELGLNEVIALLNPPGPAQEMALWQAA
                     HSVRVSVMGMCVHMRAIIEFSNYCRQDCLYCGLRRSNNRISRYRMSPDEIVEAAIEAH
                     SIHPFGTFVLQSGEDPGYAADQLAVAVRRIAETTGSAVTLSIGQMPDEDYRVLREAGA
                     DRFLLKFETSDERLFRQLKPTTSLEHRLGCLRSLRRMGYQVGSGIILGLPGQDLSSVA
                     ADLLLCRDEDYEMVSIGPFIPHPDTPLGNGESGVAGGSPKAGFDTNGTEASTGAAVLA
                     SVRTTIDAVAVARLLVPRAHMPATTALAVMGGNDASWKEIAPSPRLTDARSLALVSGA
                     NVLMLDVTPAKYREFYTIYPGKSGADGDVISDFVRQAKEDIDALNMTICPGKGDSPKM
                     PWGRGGKGVDR"
     gene            17949..19337
                     /gene="hydG"
                     /locus_tag="PLU15_02185"
     CDS             17949..19337
                     /gene="hydG"
                     /locus_tag="PLU15_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOO29760.1"
                     /translation="MTGRTECTFIDDEKIRGALEEGARYSPAAVRDVLAKARELQGLE
                     LEELAALLWVEDLALEEEIYAVARKIKETIYGKRLVFFAPLYVSDYCVNNCRYCAYKR
                     DNRQPRRRLSMDEVRQEVLAIEDMGHKRIALEAGEDPVNCPVDYITEVMETIYDTHSG
                     NGSIRRINVNIAATTVDDYRKLKDAGIGTYVLFQETYHRDTYVAVHDGPKSDYDWHAT
                     AMDRAIEGGIDDVGIGVLYGLYDWRYETVAMLAHAHHLDETYGVGPHTISFPRLRPAA
                     GVSLENFPSLVSDNDFKKIVACLRLAVPYTGMILSTRESPEFREEVIALGISQISAGS
                     CTGVGEYSRRKAHDTPQFEVSDRRDLDEIIRDLLPSGYVPSFCTACYRSGRTGEAFMS
                     LAKSSHIHEFCQPNAILTFEEYLCDYASPATVKAAEPAIRAALAEVDPKLRDECEKRI
                     ERIQGGERDLYF"
     assembly_gap    19372..19471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19533..20756
                     /gene="hydF"
                     /locus_tag="PLU15_02190"
     CDS             19533..20756
                     /gene="hydF"
                     /locus_tag="PLU15_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOO29761.1"
                     /translation="MSFDKAPRSNRIHIAILGRMNSGKSSLINTLADQDVAVVSPMAG
                     TTTDIVYKSMEIHGTGPVVFIDTPGVDDDSLLSEARVARTMRAINKADMAVVVVDAKL
                     GEGEPEAQLYSCLERKRVPYIVAVNKTDLCDADCVLCDGAGASGEEDAPRVCVSARTG
                     RGKEELLGAISRLAQSVGGLGEGPPIVGDLVSCGDMVVLVIPADAQAPKGRLILPQVQ
                     TIRDVLDHEGIAVAVRIDQLRQLFADKDFLPRLVVTDSQAFGEVAPVVPESVPMTSFS
                     ILFARHKGDFELLVEGARAIEGLEPGDVVLISEACTHHPIEDDIGTVKIPRLLNAKVG
                     GELRYEWQRGGDFPEDLDRFKLVINCGGCMLNRREMCSRLEAVADAAVPVTNYGMAIA
                     ACLGILPRALRPFAL"
     gene            21198..21620
                     /locus_tag="PLU15_02195"
     CDS             21198..21620
                     /locus_tag="PLU15_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29762.1"
                     /translation="MAISPRLLEIRSCLVHDYEPLIDYGAWRVAAICSSERNAVDGVK
                     SLERHNETDEVFLLLAGRAFLIIGEGDASVETFHAVAMEPLKAYNVKRGVWHSCVLDT
                     DSVVLVVENRDTTHLNSEFLDLPSEICHQLPDICGEIV"
     gene            22043..22933
                     /locus_tag="PLU15_02200"
     CDS             22043..22933
                     /locus_tag="PLU15_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29763.1"
                     /translation="MSRRRIAIVLVCFFILSLGLVVGSPAYCGETLASTTYYQGGKRF
                     QVDVDLGANWEMTLTVYRIELSGKPRKLWSYTGDHIELEMVRDVDGDGFVEVLAMEYG
                     GNTYACPILFRVDKNEKVRQIPIDLGKMYEKPNYMLITRAASFIDLDDDGVDELIAWV
                     PQYWMPYYANSDMVYASIICKAKDNRYVAAAGDYASVYRFLISELRNEIVTYGTDILE
                     LDVGPYLQNCCLLLLYRSLVGEMTQGLQEFDTLFPHALKVIDPVADKWFADTWKDFMK
                     RRVDLLTQATRDFGGIAQKR"
     gene            23137..23940
                     /locus_tag="PLU15_02205"
     CDS             23137..23940
                     /locus_tag="PLU15_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29764.1"
                     /translation="MERSRIYGAFERVSRKWWFYVLVVMALGIPPYASTGVAPEQIAS
                     LVQEVLSQGLTAYSALMPMLHIAVAALVVLVGIYGNRVGRLFSAFMGLHYIFIAIAQN
                     TAVTPTFGYAVISSNVLLFMVIGIMWLWDAAIGKTDYTLRGPHPWWTYWVLPLAVLAF
                     WGPDVPWSFHLRYLVTSSSVGAFCLMTPIYLSVLSFCPRYNLPAVRLTSFVGILMAIF
                     NFMIGLSLCIGGNASLGIYQVFIHLPLAALSTYSFISSFRAGRARQPCS"
     gene            24019..24507
                     /locus_tag="PLU15_02210"
     CDS             24019..24507
                     /locus_tag="PLU15_02210"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOO29765.1"
                     /translation="MEPFASLDHERYMREALSEAEKAFERGDRPIGAVIVHDGEIIAR
                     GSNRFFSESSYIAHAELNALVSAAPYLYDHGRECVIYTTVEPCVMCLGAIIHANIRNI
                     AFGMHDNYIQARKIIDAVEYVDRRVHNYLGGILEKECVELYRRYSEREAQLMLTGTQN
                     SW"
     gene            25004..25819
                     /locus_tag="PLU15_02215"
     CDS             25004..25819
                     /locus_tag="PLU15_02215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO29766.1"
                     /translation="MRRKARERDDSLCETQYEGFFSEFYDLLHSSYNADLPTYLDLAE
                     EYGDPILELGSGTGRLLIPLARAGYRVTGLDCAEDMFALCRKKLEAENGATRERVTLV
                     NGDMRDFDLDMKFNMVLAACNTILHCTTIDDLLAVLARARRHLSQKGVFVVDFAIPNI
                     LRMLQTNGAEARFEMVHPVSGTRIIDTYVAGYDFTKQMETYEARIEEWDESTMLRWSE
                     VKTERAFYFPREIELALRCSGFEIVKTWKGMRGGPLVETSQDMVYVCRLARQG"
     gene            complement(25871..26353)
                     /locus_tag="PLU15_02220"
     CDS             complement(25871..26353)
                     /locus_tag="PLU15_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29767.1"
                     /translation="MRVESLTVRICDAEIKHALQNMELPQGLKVVDVKIASGMITLQL
                     ALPYNIKFHPVICLSNPTVQGAVVALRISGPLGLSLPDIVLSSIMPVLPPGVDYLGRG
                     LILIDLLQIYEGAFRSLDLEGISIEPCCITLHVACAEFDLESLPKAASLLQTITIDES
                     "
     gene            26594..27751
                     /locus_tag="PLU15_02225"
     CDS             26594..27751
                     /locus_tag="PLU15_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOO29768.1"
                     /translation="MAYAILCLQEWKPFRRICRIKPVMCAMTGGIMVKRIAKAILIVV
                     AVALLALVAFIVVIAATDYRPCDIEPVEVENRREWVTESGAELSVTTFNIGYCGLDKH
                     TDFFMDKGTMSRSISREQTEINVDRVSQFLQEADSDFYLVQEVDRKGKRSYNIDQYKR
                     FIRDFPEYSSSFATNYKVAWVPIPVTHQYGNIYSGIATYSTRRVDSAERFSLPGQYPW
                     LKQLFMLDRCLLESRLPVDNGKELVLVNLHLSAYDQGGTIRAQQLAFVKEYAVREWGK
                     GNYVILGGDWNHLLSATPKEKKASYGDAWPDWLQLLPDDFVLEGFEWAMDEEVASNRC
                     VDRPYNPATTVVQTIDGFLVSPNVEIVAVQGIDLGFENSDHNPVTAVFKLK"
     gene            28293..28832
                     /locus_tag="PLU15_02230"
     CDS             28293..28832
                     /locus_tag="PLU15_02230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO29769.1"
                     /translation="MKRVLNKVKVATVAAVLAPLLFGLFCPYSTVAATQISEKWIGKS
                     SPDFVLRTLDSKKIALGDDPKSVYVLDFWAVGCGPCKRLAPHLQTLHSKYAPKGVTVV
                     GVNVGDSPSAISKYAKENKLTYTMAIGDARTVDAFGVYAFPTVVIIDRKGVIRDVYVG
                     YLPGWEQKMENTIKQILGD"
     gene            28837..29001
                     /locus_tag="PLU15_02235"
     CDS             28837..29001
                     /locus_tag="PLU15_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29770.1"
                     /translation="MAERSGAGPTRQVPVWALILTAGVGLMAVGARLGDFEMIFLNAK
                     LICLSCIGIE"
     gene            29005..29820
                     /locus_tag="PLU15_02240"
     CDS             29005..29820
                     /locus_tag="PLU15_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO29771.1"
                     /translation="MAIRKRKSSARSIVQALATIAANSGFPQMKICPYPGLNCYACPL
                     ASFACPIGSLQKFIATRQFPYAMTGFFATVGAVVGRAACGWVCPFGFLQDLLAKLGPK
                     RKLLRSQRGNWIRYAVLIALVGVGTYAVGQPLFCKICPAGTLEGGIPQVLTKPALRVL
                     IGPRYWVKIGLLAVMIAASILIKRPFCRFACPLGAIWSPFNKASLMRLEVDEGACTQC
                     GACKNVCPVELEVYRDPNSDACIRCLECTRCDAVSVSWSRKSTSAGKACVGGL"
     gene            30172..30603
                     /locus_tag="PLU15_02245"
     CDS             30172..30603
                     /locus_tag="PLU15_02245"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO29772.1"
                     /translation="MLAFIVIYLPDWITIVLRPVPSGKGPFLTGLYSLGRWGCVLLIA
                     MAVQDVHADEVGLGDLGSLASRAILMIIFLFGSYAWGVPCTESDLDTYVVLPENADMR
                     EIDAVKLIRKAIHDKKTMPVDVIVSKENESNRWKIAPTIER"
     gene            31568..32209
                     /locus_tag="PLU15_02250"
     CDS             31568..32209
                     /locus_tag="PLU15_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOO29773.1"
                     /translation="MAKGLKDPVSGLTHLAGAIMSVIGLVVLLRAGIANEDLWQIVSF
                     SIFGASLILLYSASAAYHLLNVPSSVELVLRKLDHIMIFVLIAGTYTPVCLGPLRGAT
                     GYVILTIIWALAIAGVFFKVFYLNAPRWLYTGIYLAMGWLIIFATGPLVRAVGLHILS
                     WLLIGGVFYSIGAVIYGLKKPDPFPGRFGFHEIFHIFILLGSLSHFRFIHLLA"
     gene            32352..33734
                     /locus_tag="PLU15_02255"
     CDS             32352..33734
                     /locus_tag="PLU15_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29774.1"
                     /translation="MRFADLIATAVRRCRARLLESFLIVLGIALGVAVVAAFSGLIGT
                     VGKEYRQFSDSIAFREICIESTSGRSGGSYSTDRPAFALSRTGEAPYELSLHDVDRIK
                     SRVSPDVYVYAYEDFFQHLRPGDDLSVGSSLDDSIPKSSENPEKGPVPRNVRVIAATE
                     EVFSAYGAEFREGIGFSRPDVASGERCMVLGSRLAERFFGDAPAVGKKMIWQNEWMDG
                     PTGELELSPSGHVYTVIGVMERIEPPVSAGKAGESPDDIDFSIDGSAYIPITSAPGME
                     GPNPKVHEIHAVSALSKDAARAMDEVRQAIETEYDGRLDASSGMEYMKESMRELRSFA
                     TGILVLASAGLVIASINILNLMLARVLRRTREIGISAALGAGRREIFRESLAESLVLG
                     ISGGVIGLPLSYGLVKLMSRIMEGLDVSVGLTGVLAGALSCIVVSVIFGLYPAYLAAC
                     IIPADALRGD"
     gene            33739..35187
                     /locus_tag="PLU15_02260"
     CDS             33739..35187
                     /locus_tag="PLU15_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29775.1"
                     /translation="MTSHLFKSAWINMSRRPMQSILTIIQVALAVACMVSVASYRMNV
                     STAVDRFKSCTDDIVIAMGGMEKRTEGGYMRNMYNIFDDDDIAQLNAEVSLVESVTVY
                     EDMWDIEADVDGTLYRIGLGALVGPGYQDVVGFDMIEGNFITDVDMKAQSRVVVISEA
                     LAKILFGEGPYVGRTLGIMPVWARRSFANEDGAMPVAPTEYHVIGVYSNSKTSTSAMS
                     SYTGMHPAILCPLTSDPNQIYSSGQGWLCMDEEYPYGTLMIKAAPGKAAAVRDRIVAM
                     VSGRPSPDSLSGSSGSMVVMGGGSAEFDGPGSDGEGSDWYSPPDEESGASVIFERTDD
                     LEQMIGSSMTKMTLLLGGATLIAIIVSALGIQSAMMVNVTERTREIGLRRVLGASRRV
                     VVVQFTVDAMILAAVGGGFGGLLSFALYPYLMNSVFSSMGPGWLLDVSARPVGWAILA
                     GIVLAAAVGALFGAIPAAQAARVQPADIVREL"
     gene            35205..36530
                     /locus_tag="PLU15_02265"
     CDS             35205..36530
                     /locus_tag="PLU15_02265"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO29776.1"
                     /translation="MAAGTPETEREYEPIVTSPARTSSSKTRRDMGMLIFIIAVLVGA
                     VALAARFLIPKEKPYLLSNYTFAHVSTADFTDSVSAPGTVAPATTVDVRADVSGVVAK
                     LSAALGDEVNVGDPICVLQSPELVTRRDEAVRQVVAAEDALAKAKLDAAQSEERVRRE
                     IVVAEQAVSDALNRREDMEKLFEAGAVSRVQVDEARAAESKARMALDNARLDHDATIR
                     SNALSIDTATRQVGVARNALRAAEDCISQLTVCSPIKGKVLELSVVLGATASQGGVVA
                     QVADMESIVVKAKVASTDVRRVTVGQPASINLGGRKVAGVVRHVAPKAEASGQGATVE
                     VTIDFDERPGDVPPNSAAFVDIEVQRRTDVPSLPRGAYLASSRELFVYVIEGSKAVQR
                     DVRFGSIFGNRIEVVDGLSVGESVITSSYEEFKDRREIKVAPEGGRESQ"
     gene            36527..37237
                     /locus_tag="PLU15_02270"
     CDS             36527..37237
                     /locus_tag="PLU15_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29777.1"
                     /translation="MNEDRQAQAFIQLEDVCKVYAMGDVSVAALGGISLEIPRGEYVA
                     IMGPSGSGKSTLLNIMGGLDVATSGKYLLEGVEVTRLPDAELARIRCRHFGFIFQSYN
                     LFPELTAIENVIVPMMYAGVAPADRTQRAAELLDSLGMGHRLRHLPSQLSGGEQQRVA
                     IARALANDPDVIFADEPTGNLATAQGEEILDILEGLNKRGVTIVIVTHSENVADRAER
                     LITMRDGRVDSDQTAARV"
     gene            38047..39126
                     /locus_tag="PLU15_02275"
     CDS             38047..39126
                     /locus_tag="PLU15_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOO29778.1"
                     /translation="MDNFPTWGRYYLPADALIRRHIESSLAWTFEARGYEEIVPPLLV
                     GLDMAVLDSSGSTFTNGLAGAEKPYAVLARDGSFFALRSDMTVPVAALAVRHLLPSES
                     PVRLYYSGSVFRPQKAGNGKPEERRQFGAELIGTDAADSVKADCEILAMAAEGLINTG
                     VRDFCIGLSDAKLFLAMLDAARVEDDAKDAIWSALASRDYVRYGELVSSLDDGGDPQK
                     ALGIDLLKRLLALESTEESLDALRRAGVCDAASELIRILDVLEERGLGKCFRLDLSIV
                     REFRYYTGVVFEGYAVGCANAVIGGGRYDKLLARFGSSAPAAGLAVDVDAVVSALQAV
                     EGGERRWNRYLWPNTRPCAKVRSRH"
     gene            39066..39815
                     /gene="hisG"
                     /locus_tag="PLU15_02280"
     CDS             39066..39815
                     /gene="hisG"
                     /locus_tag="PLU15_02280"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOO29779.1"
                     /translation="MEQVSVAQHAPVRESEVAPLNLGNCLSIALPKGRLLQPSLDALA
                     EAGLQVPSMDEIDRRLIYTTGDGSMRFILARPADVPAYVEHGAADLGITGKDALLEGD
                     WGVCELVDLGFGRCRLVVAALQGAIAPDGCGSEGAVCKLPLPRSGHTLRVATKFPAIA
                     RRHFAPKRDLCVEIIKLNGAVEIAPIAGLSDVIVDIVDTGRTLADNGLVPIEEVAQLS
                     ARLIANRASLKLKSAVIHQIASALCDRSRSI"
     gene            39914..41212
                     /gene="hisD"
                     /locus_tag="PLU15_02285"
     CDS             39914..41212
                     /gene="hisD"
                     /locus_tag="PLU15_02285"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOO29780.1"
                     /translation="MDTPIGIKTIAACDYLQQRIRRGPEDFEEVEQKVRPIIEDVRRR
                     GDQAVLDYTAQFDGADFVPESIETTSADIDHAYSVVDQGVVEALRKAADNIRCFHERQ
                     VQQTWACILDDGAIMGQAIRPIERVGIYAPGGAAAYPSSVLMCAVPARIAGVQSVIVC
                     TPPGPGGVANPYVLVAADIVGVDKVFKVGGCQAIAAMAFGTESVCAVDKIVGPGNAYV
                     TAAKRLVFGYVDIDMLAGPSELVVLADMSADPRFVAADMLSQAEHGPDSEAILVTTRG
                     EVAASVVREIAVQVESLSRRDTIIRSLSEHGLIIIADDLDQAVDVVNRYAPEHLEVMV
                     DDPHALLSRLRNAGSILLGPLTPVAATDYAAGPNHVLPTGGTARSGSPLSVRDFVKIQ
                     NITSLTRVALDDMMDAAETIAAVEGLDAHAAALGARRVGL"
     gene            41209..42387
                     /gene="hisC"
                     /locus_tag="PLU15_02290"
     CDS             41209..42387
                     /gene="hisC"
                     /locus_tag="PLU15_02290"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO29781.1"
                     /translation="MIASVAGRGGANERDLGSVAEFARPELQGLRACVPDWHGVRVKL
                     DSNESPFDLPACIKENALRKLREIPFNRYPQGLAAKLRETIAFRDGVSETQVTLGNGL
                     DEVIYIILQAFGPGRKVIIQKPSFTTYTYAALSASADIRAVPLDEGLHLSTAGLIEEA
                     NAAESPCILIICNPHNPTGGRFMEEDIVDVLEATNSMVIVDEAYVEFSGGSVLPLVAE
                     YDRLIVLRTLSKAFGLAGVRVGYSVSSSEIAAELDRVRQPFNLDSLALVVAQTALEND
                     SYCREAVIRIVEERERLLGRLGEIHGVTCFPSCTNFILFRTTMPDATMVRDALRRYGV
                     SVRVFPDEPMLRGCLRVSCGTKEEDDAFLEALEKVLAEANQTSAGLRDDDGKRIEGGE
                     "
     gene            42389..43003
                     /gene="hisB"
                     /locus_tag="PLU15_02295"
     CDS             42389..43003
                     /gene="hisB"
                     /locus_tag="PLU15_02295"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOO29782.1"
                     /translation="MPKPRLAEVHRKTSETSVELRLILDAARDHGCSGRHQANISTCC
                     GFFDHMLRLLAFHAQFSLDLHATGDIEVDYHHLVEDVGICIGEAFCEALGDKQGIARF
                     GSALVPMDDALSMAVVDLSGRPFLHFNVPMPCAKVGDFDTELVEEFMRALASNGKFTL
                     HVDLLHGWNTHHIIESVFKAVALALRQAAAAGDTSSIPSTKGVL"
     gene            43005..43694
                     /gene="hisA"
                     /locus_tag="PLU15_02300"
                     /pseudo
     CDS             43005..43694
                     /gene="hisA"
                     /locus_tag="PLU15_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
     gene            43710..44576
                     /gene="hisF"
                     /locus_tag="PLU15_02305"
     CDS             43710..44576
                     /gene="hisF"
                     /locus_tag="PLU15_02305"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOO29783.1"
                     /translation="MRAAGSRRIIPCLDTKDGRVVKGVNFENLRDAGDPVGLAEIYES
                     QGADELMLLDLSATAEGRRTALDLVGRVASAVSIPLSAGGGITSLDDVGRVLDAGASK
                     VCINSAAVRNPQLLQHAAKAFGVDRIVSAIDASAIAEPFLDAGNRHGDRDVNGIVSIE
                     VDSKSDGNGDGCGRWVVCTFGGKQRTDLDLIEWARTVERLGAGEILLTSVDRDGSTDG
                     YDLRQLRAVTQAVGIPVIASGGAGTPEHFRDAFVEGGADAALAASVFHFGTLSVGDVK
                     RHLKREGVDVRL"
     gene            44573..45286
                     /gene="hisIE"
                     /locus_tag="PLU15_02310"
     CDS             44573..45286
                     /gene="hisIE"
                     /locus_tag="PLU15_02310"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOO29784.1"
                     /translation="MIERLESDDGESLEGWIDTLKFGADGLIPAVIQESVTGQVLMVG
                     YMNRQSIEMTLREGRTCFWSRSRQRLWTKGETSGHFQKVRAVFCDCDRDCLLIEVDQT
                     GEACHLGRKSCFFTQLWGSADAAGTGFSLDKLYETVEDRKASQPEGSYTAKLIRSGQD
                     RVLKKVAEEAGEVMLASKNDDREEIIREMADLWFHSIVVLAYHDIPPQAILDELADRH
                     SNAWKAESLCTERKEGPGY"
     gene            complement(45327..46841)
                     /locus_tag="PLU15_02315"
     CDS             complement(45327..46841)
                     /locus_tag="PLU15_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO29785.1"
                     /translation="MSQTAQHKSQQRQTAFVTVPFSRRAADICICVVMLLLALAPCTF
                     AADSSLGASHADESLALTMEDVMRLAESSSPSVATAKRAVELAILQLQAAEAQKRPSI
                     TVHAAPLQIGIGPLSVQSVVEEDSKPMGKGETSMYALSQAGGDVRIPLGGGSLSLSAT
                     ASFAFSDPALSGKSAWNGSASLSLPILGASSPGEAPSSTSSAQSQLRSAQFQLDQSIR
                     QARASAQAAYFNLLAAEDRVAAADRGLSRARTHAAFVEGRCSAGNASELDALEAKAGA
                     DRAEAAAQSARHNLAIASISFNRAIGRDLDTLVHLAPAGEYVRWPEDLDLDTCTRAAL
                     ETRPEIPMAEQDVADAEAALAQAIAAKRPDVFFQGQASSGGHWRVGLEVSALLTRDYA
                     AEIAIESAKGRVEQAKANAVETRDSILLEVVEAFYNLREAESSVELARLAVAIATATL
                     DIREEQCRIGAVAYAEVSEAIDAVRKAESDLAYQLAACFMAKSRLLQSVGSDNI"
     gene            complement(46838..47986)
                     /locus_tag="PLU15_02320"
     CDS             complement(46838..47986)
                     /locus_tag="PLU15_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO29786.1"
                     /translation="MTIKPLSKSLRLKNPAHARHFRQSPLAVTMAVLALLALSGSGGL
                     CAVAQPEPLELTLDEARSYAIDHSYDYAMAVLEFEASEIQFQMAEADALIRPSVIASK
                     RAAGARRDARRALVAKEQSLMLDVDEAYYNLVTAQERANIIHASEEQARESLRIVRLR
                     FEAGLASKMDVMSAEIALEQAVTSTLSAKNHLELAQLALKRTLGMDLDTPVIAIDSAQ
                     PIAGDVDYEAALALALKNRPEIVKARETLEICELEAKFADNEYTPELTKRQLNNALAQ
                     ARLAAGQAERGVRVEARQMYLSLEESHRAISIASAATKQAEENYRIMKLRYEHGMEIA
                     NSLLGAQLSLTEARLSELQSIMSYNIARLHFDAWANYPAEEAEPGDPR"
     gene            complement(48339..49517)
                     /locus_tag="PLU15_02325"
     CDS             complement(48339..49517)
                     /locus_tag="PLU15_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO29787.1"
                     /translation="MAKIYLDNAATSFPKPPQVWEAMQQYMLNIGASPGRGGYSLSLD
                     AGRLTLNARIAAARLFRAPSEEHVVFTPNVTYALNCAIHGLLRPEDHVVTTSMEHNSV
                     IRPLCHAKDQNRVEVSIVGCDSLGRLDPDDIRKAIRPNTKMIVMTGASNVVGTILPVA
                     DVGRIAAEAGAFFVVDAAQTAGVIDLDFGALGADILAFTGHKALYGPPGTGGMIVSDR
                     AAAEMLPLAQGGTGSKSDEERQPGFLPDKFEPGTPNTVGIAGLAAGMQFVQKTGPDRI
                     RRHEAELASRFVQQAVDIGPKVRVLGPWGSGEASADDAVATVSISFEDGCIDPGRLAY
                     ELDAEFGVMVRSGLHCAPLAHKTVGTFPEGTVRFSFGWFNTIADVDRAATALAELASE
                     "
     gene            complement(49553..50533)
                     /locus_tag="PLU15_02330"
     CDS             complement(49553..50533)
                     /locus_tag="PLU15_02330"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOO29788.1"
                     /translation="MDIKQLSIFLAVANHGSFTEAAVSLFMSQPTISVQIAALERELG
                     VTLFERKSHGVTLTPAGQVLKPYAADILAMRDRAAEAIDHYKSDFSGTLTLLASSVPA
                     GYVIPEFVAEFLAEYPKVFIQLRHADSQEVWDRILNYDADLGIVGSIGNEASIESFPI
                     IEDEVILIAPPKSKYAAWGDIIDIESLLAEPIVCREPGSGTQVTFDKALQEHGIDPRG
                     LNIRARLESAEAIKAAVAADAGLGLGIVSARAAAAEIAERKLKAVRIKGIDLHRQLYM
                     INHKRKVLSPTALAFRDFVLERLLHPRESTADELEMTRKRHMMQKERDYS"
     gene            50831..51931
                     /gene="yedE"
                     /locus_tag="PLU15_02335"
     CDS             50831..51931
                     /gene="yedE"
                     /locus_tag="PLU15_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOO29789.1"
                     /translation="MNQRRVVIVAGAIVGALSIILVATGNPGNMGICVACFIRDIAGA
                     LGLHRAPIVQYIRPEIPGFIIGSMIAAMAAGEFKTRGGSSPLLRFVLGFFVMIASLVF
                     LGCPLRMVLRLAGGDLNALLGIAGFAFGIWIGTLFLKRGFTLGRSTAMHKGNGAVMPL
                     ISIALIALLVAAPAFIFFSETGPGSMHAPLILALLAGIIVGVVAQRSRLCLAGGIRDL
                     ILIRDPHLFNGFVAIFAVAALLNLLTGRFQFGFDGQPVAHTDGLWNFLSMSVVGLGAV
                     LLGGCPLRQCILSGEGDGDAAIAFLGMLVGAAFAHNFGLASSPAGVTGAGKAATLIGL
                     AVMLAIGYFGAVNAVDALSTVGGGKKTAGGSR"
     gene            51944..52174
                     /locus_tag="PLU15_02340"
     CDS             51944..52174
                     /locus_tag="PLU15_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOO29790.1"
                     /translation="MATQVDARGLSCPQPVVLTKKAMDSITSGEIEVLVDNPAARENV
                     ARFARNAGCSVEIGEERDGSYVISISKAGRKS"
     gene            52137..52679
                     /locus_tag="PLU15_02345"
     CDS             52137..52679
                     /locus_tag="PLU15_02345"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOO29791.1"
                     /translation="MSFRFPRQDVRAKQNNVEPPHEAESYESRAGASGPASCVAGCSF
                     GVGRGVEGAPKREKEAAESAEEVELLVTFPSVHHALKLERMIKTCACGQDGSGRSVDL
                     MPVPRRISSSCGLAASVCVTMGAGSTSGNGVARLGSPGTVCRSLVEELKAAGVEYQGI
                     YLPLPGGEFQLLTDGEEAGM"
     gene            53190..55028
                     /gene="pepF"
                     /locus_tag="PLU15_02350"
     CDS             53190..55028
                     /gene="pepF"
                     /locus_tag="PLU15_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002089927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOO29792.1"
                     /translation="MELRIEIGGGSGLNRRPLRSEVPVEETWRLEDVFGSDEEWEAAL
                     KSLDAGIPAVAQYMGRLKDGPRLMAECFEAAENLIRRAAPVGLYAYLRLAEDGSNPAN
                     QAMMGRSAAMQAQIGAALAFIEPEVLSLPDGTVERYLKEEPRLHPFRRRLERILDDKP
                     YVLTFETEAALAMLGEVMNAPQMLYERAKSSDMHFDSVVDSEGKEIPVSFATYEVMLE
                     KSPDVTLRRNAYASFTEGLKKYRNVLGGTFGTEIKKNVVMAKMRGFDSATHMFLHQQE
                     VPIDVYENLLNIIQEECAPHMRRYVGLRKSALGLDKILYCDIEAPLDPEFDPEISYEE
                     GARLIIDAVQVMGAEYAEIVQMALEDRWVDRVDNVGKSTGAFCAPAYDSHPYVLTTWG
                     GHMRSVFTLAHELGHAVHSSLTARNQRLVNTEVSRVFVESPSTFNEMLLARHILSKTD
                     DKRMRRYVLMQLLGTYYHDFVRHMLEAELLRRVYALAEKGQPITASVLSELQGEILDG
                     YWQGLVEVDEGARLTWMRQPHYYMGLYPYTYSAGLACSTAVAQALDEGNTQEVAGRWV
                     HVLKSGGTQKPLDLMRTAGVDLEDAGTIRGVVDYVGSIVDQVEETM"
     assembly_gap    55036..55135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            55171..56985
                     /gene="pepF"
                     /locus_tag="PLU15_02355"
     CDS             55171..56985
                     /gene="pepF"
                     /locus_tag="PLU15_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOO29793.1"
                     /translation="MAERLARSEVPVDETWRLDDMFQSMEAWEAELAAVERIIPQVAE
                     FQGRLGDGASVLRKCYETVELLARRFEKISTYAYLRFSEDGTNPVGQELMGRTMAAQA
                     RLDSAIAFMRPEILSLPDGTLEGYFSEEPGLETFRRRLELILSDRPYVMSAETETAIA
                     ALREVLDAPKMVYEWTKTSDISFEPAVDSEGHEVPVSFATYEEILEVSPDAALRRSAY
                     TSFSRGLARYQNALAGTFAAEVKKNVALARIRGYESATHMFLHQQEVPVEVYHNLLDV
                     IEEGLAPHMRRYVDLRRRVLGLDKIMYCDIEAPLDPDFMPEISYEEGARLIIDSVAVM
                     GAEYADIIRVALEDRWIDRADNIGKSTGAFCTSTYDVHPYILATWGESMRSVFTIAHE
                     LGHAVQSVLASSNQRFVNVEPSLVFIEAPSTLNELLLAEHILESADDFRMRRWVLMQL
                     LGTYYHNFVRHLLEAELVKRIYAHSEAGRPITAALLSAEKGDILECYWDGVVEIDDSA
                     KLTWMRQPHYYAGLYSYTYSAGLTCATVVAQSVREEGSRAAERWVNVLKQGGSMAPLE
                     LMKTAGVDLEDAATIGDAVDYVGRIVDEVDRGFGGQPH"
     gene            complement(57084..58907)
                     /locus_tag="PLU15_02360"
     CDS             complement(57084..58907)
                     /locus_tag="PLU15_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO29794.1"
                     /translation="MRGIGVDFGTANSMLATFDGKSAEALLADGHGHPSLVWYQKRRQ
                     PRVGTEAKDAYSSLLGDPDHAFIKSVKRRLASDDTVTLATRTISTETATEVIFNFLLK
                     EAKISPEQDNRFVIAIPNSLTRDQRKEIMRAAKAAGFSPSFLVSEPLAALVGYLEREP
                     IDASECRRVVVADWGAGNVEITLVIMTDDCLVEVASCSLDDVAGDDIDALLAEEALKR
                     FMKRNGLSRQKLEEAMPGLAPEEALLSAAEQAKIALSSDSETQIAKAGFCSVDGVDYD
                     IEESFSKAEFEDLIRPIVDRVLQATEEMLASAGAKPSDVDLCLLIGESTNLPPVVEGF
                     GRLMTPDRIVRPSASSTLIAEGAAIMAYRNCIPRLVSDISLLLADGSEHELIPAGTAM
                     TPKEESEAPEEFICVDPRQGKIVTAVRANPSVLSSVSDICDRSEHLVELEIDYVQTYR
                     VYNLPRLFVEAGFDETCMLRIDFSSSEAKVEEGLEVEAASPRAALFNWSKNDDGKLDS
                     PSADACRVIPSPTTCESISNILPITAGKRRIWASVPVELLIELEPEIRDVRLMEKYLT
                     PIQRDEYLNSLSSIPAYSGHHEPADSACESDGEPESKSSNS"
     gene            59366..60970
                     /gene="murJ"
                     /locus_tag="PLU15_02365"
     CDS             59366..60970
                     /gene="murJ"
                     /locus_tag="PLU15_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOO29795.1"
                     /translation="MSGDASKTLAKSAGIVALGSVLSKILGFLRESALASEFGASYAT
                     DAYLMAMIIPTLMLFGVGPAVTTTLIPVFTDLEKRKGRDAAFKSVSCIINACIVVAAA
                     VTVAGLVFAEPLVSFVAPGFSGETYELTVDLTRLLFPIAIFTVITHCITGVLHALGRF
                     AVPAMTGLVQNVIIITSILVFGPKYGITAVAVGTALGAASMFLVQLPALFSSGFRYHF
                     ALDWHDQGLRQVGKLIGPIVLGSVAGQAGTVIIRILASRLPTGSITYLNYSQRLVGLP
                     VGVFGTALVTVLYPTFARMHSEGTREDFAKTFRRSVGVVFFTLVPMAVGLAVLATPVV
                     RLAFERRAFTPEATAATAAALVYACVGIPAMSLSDLSSKAFYAMQDTITPVLVNMGAV
                     GVNVALSLALVEPMGHTGLALAGACQPIASFLMLQAVLRYREKKWTRKARTDGVADNY
                     SLAGSIAKSVVSVVVMWFAVSYFDAWITEVFPGMGTGRQIVRLAASVGVGAVVYFAMA
                     AMLKSQELSFAFSTAKKRVKERKTAV"
     gene            61162..62037
                     /locus_tag="PLU15_02370"
     CDS             61162..62037
                     /locus_tag="PLU15_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOO29796.1"
                     /translation="MRVHLDCIACTLRQALEAARLATADIDVQERIIRRSLETLADYD
                     LYRTPAELGSAVHELVKECSGNPDPYRNVKKATIEMAHAIYPGLKRFVEEQDDRLLVA
                     LEVSAVGNLLDAGVYGDLRTVDLEETLKEELSRGFAVCDIDFLRADLLGATKVLIIGD
                     NAGETVLDRILISEIKRACGKEDKVNVFYGVRSAPMINDATAEDAVASGLDHDATIVD
                     TGCCTPGLVLRNATPDFLKLYEEADVVISKGQGNYETLSDSAGRTIHFVLKAKCRAVA
                     EDTEVNIGEYVLLRR"
BASE COUNT        13565 a        15302 c        18520 g        14452 t
ORIGIN      
        1 tacggggcca gtgggctttg gtaactgtgt tctgaatata tgagccgccg cgtcttaagg
       61 agtggtgcag atagagtctg aggtacgcag gttgaactgc agccgtgaaa tactcaccgg
      121 gcttttgagg aaagcccggt gatatgggaa ccgagaatta ccgcgccgga gaaatatcaa
      181 atcaggaaag ggcagggggc gaaattgccc cctcttattc tattttgcct gctattggcg
      241 cccggcgggc tttgctatcc gcagctactt gcctgttata gagcacccct caatctggat
      301 gtaaggcatt cttgagccgc caacatcgac gtattcacgc gatacgtcca cgacctgtct
      361 gaggaggtcg tagatgttgc ctgagatcat gacttctttg accggacaca cgattttgcc
      421 cgcttctatg taatgccctc cctttaccag gcccgagaag tcaccgctca cagggtcaac
      481 attccctgag aacctgctca ccagcagtcc cttggaacag cccgctatca tctcgtccag
      541 tgagtgtttg ccggatgcaa cgacgaagtt cgtcgtggaa atgcccggaa cagtatggtc
      601 gctgcctgct gcgcgcccat tggaggcgac gccatctgtt ttagcagtat agcagttata
      661 gaggtaatgg ttgagcgtgc cttcgcttac gagctgcaaa gcagcgggcg gcaccccttc
      721 gcggtcaaat gaggtggacc ctgccgcttt gggaagcttg ggatcatcag tcactgaaat
      781 caccggcgac attacctcgg tgcccaacat gcccgcccat ttggatctcc cgttctgcac
      841 attctgggca ttgcatgaga acgatatggg ttcggctatt agatcgtagc ctgcgtaggg
      901 agtgatgatc acgtcccctt tgaaactcgg cacggtagtg gccccgagcg aagcgacgac
      961 tttctcggcc agtttgcgtc ctatggctgt aggatcgatt tcgtccttcc tgcacgtgac
     1021 cgcaacttcc aaatcgaaag atgacacttc tttttcgtcc ctggcaaacc ccatagctag
     1081 gcaggtgaag acagaatctt tttcaacgag cctgatgccc ttggagttga caatggcttt
     1141 ctcagtgatg tccaccatat actgcgctga atccaaagta actctgggat cgaaggcacg
     1201 agctgagttc acaagcgcta acccctgctc tactgcgtct tcaaggctca tggtttcagc
     1261 agattcgtcg tatcggccgg gaaccggctc gatcgtggac ggctcaggca tccagtgatt
     1321 gggatcatca ggcgacgcat tcgctatcgc tatcgcggcc aggagcgtct cctcaagcga
     1381 gtcgggggag agcatgttcg tggcggcaaa gccttgtttt gctccgccct tgccgtaaag
     1441 gagtacgcgt atgcccaggc cgtcatagtc gtcgcccttg ggtacctgca ggtcgttttt
     1501 ttcgaatact gccccaagtt gtctggactt gatgtagaac acttctgcat cgtcggcacc
     1561 catcttgcgc gcacgttcta ccaggctgtc acagagctcg agttccttca atgtttcgct
     1621 ccctcctcta ctcatggcgt cctccgacgg ttacacgaca acgcagataa ggccctccag
     1681 cgtccacctt ggcagcttgg aatttgccgc agtgtcctgc gcccatcgcc atctcaaaat
     1741 cggaggatac tgcatcgacg ctctcaagaa catcaaatgc ctggccggag atggtgatgc
     1801 ccttgaccat ctctccaacc ttcccgtcca ctattcggta ggcctcggcg acgccgaaca
     1861 tgaactctgc cgtggcgtcg gcctggccgc cctgaccaag ttccttaaga aagaacccgt
     1921 cgtctatccc ggcaatcatc tcgtctaagt tgtcctcgcc gggtagtatg aaagtgttag
     1981 tcattctgat tatgggctca tcgcgataag tgaaggcccg cgcattgccc tttggctcca
     2041 ttccgaaggc tgctgctgat tcgcgggaat gcaggtaatc tctgaggatg ccatggtcga
     2101 tgatgaccgt gcgctttccg gccacaccct catcatccac caaaaccatt ccggcggcgc
     2161 cggctacagt cccgtcgtcc accatggtca ccagctccga ggctacttcc ttcccgatct
     2221 tgccattggc aaccgaaccg ctcatcacga aatcggcttc tacggtgtgg cctatggcct
     2281 catgcgatag gactccaacg agccccgggt cgagtatgac tatgtactgt ccgcctttgg
     2341 catactccgc atcgagcttc tttacggcca ggtccacagc ttcatccaca agtgtgttga
     2401 ggtcgccctt gcgcatcaaa tcagcccagg agcctgtaac gccagcgctc ttgatcgccg
     2461 tttcaagctg gccatcgcgc ccggcagtgg cgaacacatg gaagctgggc ttgttatcga
     2521 gcacatggac ggcggacccg tctgaggtga ctatgtactt ctcgtcttca tactgtgtat
     2581 accttgtaat gctgcctaca atgagaggag atcgctcgcg aaggcgcttt tcggcctcaa
     2641 ggagtgcgcg aatcttctct gcgatgtcgg gctcctcggt tgcatcgtag tagacgagct
     2701 ctccttgggc aggttcaaca gcagccagtt gagctttgtc tttccggact gccgctccgg
     2761 cccgggctgc ggcttgggct tcagatagag cctttttcag ccccgactcg gtcacgtcgt
     2821 tagtggagac aaatccccat cctccgtccg ctaaaactct gatacctgcc cccgccaggg
     2881 tcctatgctc cacctttgtg agcgcgccat ttcgcgcttc gatgatatcc tgaatgcgct
     2941 tgtgatatct gatttctgcg tagtcgttgc agcctgagat gactgacttt aacaaatcta
     3001 gcattacatc accctccttg aataagtact gtcttcggtt cggggcgcaa tagctgggtg
     3061 aacagggtcg aaccgaagat gctgggaagt gccgagtgct ttcagagatg cccgaaaacc
     3121 atgtcacatc gccacaccac gacgttgccg caaaatggtc agtgcaggga ggcgcgttca
     3181 ctggggaggc tgcgcctgcg ccggtctagc cggcgataca tcacctgtcc ggtaaagagc
     3241 gggagttctg cgggtttgac aatgcgtcct ctaactccgt taaggtatgt atcatcgtga
     3301 tttccggtaa tgtcaggttt tcatctttcc ctttcagcca gatgctgtcc gtgcccgcct
     3361 ctatgcaagg gagtacatct attttgatat tatctcctac atagacgcaa tctcaaatat
     3421 caacattaac tcttttttgg cctctgtcct tcgagttttc ggaaagaaga ttcacttcat
     3481 aataacgtct gtagagtctc aattgtttgc cggctgagat ggttttgaca acatgccaga
     3541 actgcttgag gaaagcgagc gcgcactctt aagctttttt tgcccacgag cggcttgtag
     3601 aagcatccta cagcgtcgcg cctgcccctc ctgagaagaa tccctgatgc tcttcctgca
     3661 atggccggat gtacggtaag atggtgttga aagatgcact tggaatgagg aggggcgggt
     3721 gaatggctga caaggctgaa gttttaaacg atgtactggt aaagctgttc aacaagatat
     3781 tgtcgatcga ggaagaggcg ataacgaagg cagccgcttt aggggttacg atgtctgaaa
     3841 tccatgtcat cgaggcaata ggcacggatg agcccaagtc gatgtcggaa gtcgcctcaa
     3901 ccttaggcgt aacaatgggc acgctaactg cttctgtcaa cagattggtt cacaaaggcc
     3961 atgtctcgcg gttccgtccg gaggaagatc ggcgagttgt tcttgtgacc ctcaccgagg
     4021 atggtcgtct tgctcattca atccatgaga ggtttcatag gcggatgatc aatgggatat
     4081 tgcaggatct tgatgaggag agccaagatg ctatcatagt ggctgctgag aagctatacg
     4141 agtttttcga acgggtgaag cctgaagact tgctgctgga taacgattcc cggcagtgac
     4201 cccaatgttc tgtccccaag acattttgcc cccatcaatt gcacttgaca acgtcttgct
     4261 ttggccttaa actttgaata acaaacaatt tgaagcacaa agtaaagaac gcggcatgct
     4321 gctttctggt cttgggcgaa cctccaaggc aggagcggca ggcctcgggg gcatgcaaga
     4381 tgtacacaaa gatcctggga accggaagcg cacttccgaa acaacgcgta accaatgagg
     4441 atctggctat gctcgtcgat acgtcgaacg agtggataac gtcacgcacc ggcatatcgg
     4501 agcgatggat atccactgga gagagcgcgt catacctggc tgcagaagcg gcagccaagg
     4561 ctcttgcaga cgctgatgtg gagccttctc aaatagacct ggtgatttcg gccacagtat
     4621 cacctgacaa cttcactccg actgtgtcgt gcgaggcgca gaaggccttg ggcctttcgc
     4681 gggcgacatg ctttgacttg tgggcgggat gcaccgggtt tatcttttct ctaatgacag
     4741 ccgatgcgct ggtgaaatcg ggcctctttt cgcatgcgct cgtgatcggc gttgaagttc
     4801 tttcaaaact ggtcgactgg caagatcgta gcacatgcgt cctcttcgga gacggggcgg
     4861 gagcagtggt gttaagctct tccagccagc caggccttgt gactcatgta tccggctctg
     4921 atggaacccg aggcgacgtt ctcaagatag ccggtatccc actggctaat ccgtggatgg
     4981 ctcaacatgt tggggagtcg gagacaggct cccagagcgt gatctgcatg aatggccagg
     5041 aagtcttcaa gtttgcagtc aaaaccatgg aatcgtctat acgccgcgta gtggagctgt
     5101 caggctctag ccttcacgac atttcctaca tcattcccca ccaggccaac aacagaatca
     5161 tagaatcggt tgcgggcagg atgaaaatcc cccgcgaaaa attcttcaca aacctcgatc
     5221 acagagggaa tacgtcctcc gccagcattc cgatggccct ggatgagatg aatcgagcgg
     5281 gcctgctctt gcccggaatg catctggtgc ttgtggggtt tggcggaggt ctgacgtggg
     5341 gcgcaagtat ggttcgatgg acgcggtaat gataaggctc cgttgaaggg gcgacaatac
     5401 cttgacataa cgagaaagaa gagagagccg caaggctgag gagggttact tacatgacat
     5461 tggatgaatt gaaggcgatc atctccgatc agctggaggt atccccggaa gaaatatcga
     5521 ctggaaccag attcgttcag gatctaggag ccgactccct cgacatggtg gaactgatca
     5581 tgtcgatcga ggagaagctt ggggtccgca tagagcagca ggaagtgaag gacatggtta
     5641 ccgtgggaga tgtggtgtcg tacatcgagg cgcacaagca ggctgtgaag taggcgggcc
     5701 ggatatggta tgaggtgggt gatgtaagga tgcctgtgag gcgcctggga gagaagctga
     5761 cgcgtctttt tggcatagag tatccggtgc tgcagggcgg aatggcttgg gtggccgatg
     5821 cacggctggc ggctgcggtg tcgaacgccg gaggacttgg gattattgca tcggctcact
     5881 tgtcccccga tgagcttcgg gaagagatcg aattggctag aagcttcgcc ggcagtcatc
     5941 ctctgggagt caacataatg ctggcaagcc cgaatgctat ggatgtcgcg agggtgtgcc
     6001 gtgagaagaa agtcgaggtc gtgacgaccg gtgctggcca tccaggcgag ttgatagggg
     6061 aaatgaagga tgcgggcatc ctggtcattc cgttggtggc ttcaacggct caggctaaaa
     6121 gagcagtggc tgcgggcgct gatgccgtga tagcagaggg ctacgaggcc ggagggcata
     6181 taggcgagtt gaccactatg gctttgacac ctcaggtagt cgactcggtc gatgtgccgg
     6241 tggtggctgc aggaggtatc gccgatggcc gtggcatggc tgccgccttt tgcctgggcg
     6301 cttcaggggt ccaggtaggc accgcattcc ttgcggctcg cgaatgcact gtccatagca
     6361 actacaagcg caaggtgctg caggccaagg actcagacac tcaggttaca gggcgcagcc
     6421 tggggcatcc tgtgcgcgtg ctgaagaacc gattctcgcg cgaatttgcc cgcatggagc
     6481 gcgaaggcgc agatcccgac aaaataacgg cttatggcat gggcagactt gctgccgctg
     6541 tccgtgatgg agacgtggaa tggggctcgg ttatggccgg ccagatcgcg gggcttgtaa
     6601 ctgcggaacg aacggctgag gagatcgtgc gcggtctcgt ggaagatgcc gcgcgtgtcc
     6661 tggaatcgtt gcgagtatgc caaaaagggg agtgttgccc ttgaagatag cttacgtctt
     6721 tcccggacag ggagcacagt atccggggat ggccaaagaa atctacgatc agttcgcgtc
     6781 ggcccgtcaa gtcttcaaga ctgccagcag aactctgggc tacagcatgg agcgcctgtg
     6841 ctttgaggaa ggcgacagac tgaacctgac tgagtatact cagcctgcac tggtcactgc
     6901 gagctgtgcg gttctggcgg ctatgcacga gtccttgaat ctttcggcgg atactgcagc
     6961 agggctaagc ctgggtgagt attctgcgct cgtttgtgca ggggcagttg attttgccga
     7021 cgctgtgatg cttgtcagca agcgcggacg gttcatgcag gaagcctgtc cggccggcgc
     7081 cggtacaatg gccacagttc tcggactcga ccgcgactgt gtgatagaca tatgccttga
     7141 cgtacagaag gcaggcgcag gcatcgttga acctgccaat ttcaattgcc ctgggcagat
     7201 cgtcatctcc ggcgagacgg cagcggtgga acgcgcggct gagcttgctc agtcgagagg
     7261 cgctcgccgg gtgataagac tgaatgtatc agggcctttc cattcgagcc tgctttctgc
     7321 ggtgggaacg cggctccgca gcgaacttgc gcaggtggat atcacaagcc ctacaatccc
     7381 agtttactcc aatgtagccg cgagaccttc aatggacccg gatgaaatcc gggagttgct
     7441 tgtgaggcag gtggccagtc ccgtactgtg ggagcagact gttgccaaca tggctgaagc
     7501 tggaatagac gttttcatcg aggttggccc gggcagaacg cttacaggcc ttgtgaagaa
     7561 gactgtgccc catgcggtgg caatgaacgt ggaagacttg tcttcccttg aggcgctcag
     7621 agagtttttg gccgagcatg catccgtgca tcttccctct caggtagcgc aggttgcggc
     7681 tgcagcactt aaaacggagc ccgcgtcctt gcgaaaggtt tcgtacgcaa cccaacccga
     7741 gctcgtgtct tcttcgaaga gggcatatcg atgactgcgc cgagactgga caacagagta
     7801 gcccttgtaa caggaggggc ccgggggata ggccttgcca ttgcccaacg gcttgccgct
     7861 gacggcgctc gcgttgccat caattattgt tcctcagacc ctcaagtggt tgacaaggcc
     7921 gtcatgtcta ttgaaagcgc agcggccggg gctgggcaga aagtgcgggc ctatagggca
     7981 gatgtgtcca aacatgaaga ggttgggcac atgtttgagc aagcaatgtc cgagttgggc
     8041 cgtctcgata tcgtggtagc caattcgggg ataacccgcg acgacttggc tgtgcggatg
     8101 gaatctcagg ccttcatgga cgtgatcgag acgaatcttc tcggcgcttt tctctgtgcc
     8161 aaagaggcgg cccgggccat gttgcggcag cgatcaggca ggatcgtgct ggtctcgtca
     8221 attgtcggac tggatggaaa tgcgggacag gcaaactatg ctgcgagtaa ggccgggctt
     8281 gtggggctgg caaagtcgct cgctcgcgaa ctcgggtccc gcgggatcac cgtgaacgta
     8341 gtcgcccctg ggttcatcga caccgacatg acgagggccc ttccagagaa gatgcgcgat
     8401 tcgtgcatcg cccgggctgc tctccgcagg atcgggtcgc ctcaggaagt ggccgatgcc
     8461 gtagcgttcc tcgcttctga agaggccagc tacatcacgg gccaggttct tcgagtagac
     8521 ggagggctcg ttttgtaact tgatatttct cgggtgccca gtatgcgggc gtaattgagg
     8581 tgtttgttat gagaagacga gttgtggtta ccggaatggg ggcagtgacg cccgtgggat
     8641 gcggatggcg tcctttctgg gaggccatca gttccggaca atcaggcatt cgtcggattg
     8701 agcggttcga tccgagcacg tgccctgcta agattgcagg ggaggtgcgc gagtttgatc
     8761 cagaccggta tcttgaccgc aaggatagaa agcgaatgga caagtttgcg caatatgctg
     8821 cagttgctgc cagtcaggcc atggaggatg cgggttttgc ggcaggggga gcgccggccc
     8881 ctgagcggct gggtgtggcc ttcagttcgg gcgtgggagg tatggagaca ttcgaagagc
     8941 agcataaaag gctcatcgaa cgtggaccag agtgggttag cccattcttc gtgcctatga
     9001 tgatcgccaa catggcggcc ggcaatattg ccatcatgtt taacgcaaaa ggtccaagcg
     9061 aatgtcagac aactgcctgc gcggcgtcgt cgcacagcat tgccgacgcc tacaggctca
     9121 tagcagaggg acgggctgat gccatgatag cagggggcgc cgaagctgcg atcactccgc
     9181 ttgccatggc tggatttgcc aacatgcagg ccctatcccg aagtgaggat cctcaagctg
     9241 cgccatgtcc tttcgatcgg cggcgcgacg gattcgtgat gggcgaagga tctggcgcat
     9301 taatactgga ggagcttgac tctgcattgg cccgaggggc taggatatat gctgagatca
     9361 taggttgcgg gttttccaca gatgcgcacc atattaccag tcctcctcct ggaggggagg
     9421 gagcggctcg cgccatgcaa gaggcgcttc atgacgcgtc attggcgccc ggcgatatcg
     9481 actacatcaa cgcccacggc acagggacgc cgtacaatga tctttatgaa acccaagcta
     9541 tcaaggcagt cttcggagac gatgcctatc ggctcgcggt gagctcgacg aagtccatga
     9601 ccgggcacct gttgggcgcg gcaggcgcag tggaggctat tgcctgtgta ggcgcgatca
     9661 cagaaggctt tgtgccgccg acgatcaact acctggagcc cgaccccgaa tgcgacctcg
     9721 attacgtggc aaacgaagga cgaaagatgg aggttcgcag agccatgagc aattcctttg
     9781 gcttcggcgg gcacaactgc tgcatagttt ttacccgatg ggaataggca gaatcggatg
     9841 aatcggacgg aggcgaagga aatgggtgag acgggccagt tgcgttcgct agatgatgcg
     9901 cttgcattac tcccccataa agacccattt gtatttgttg acgagatcgt ctatttggag
     9961 cccggagtca aggctgtagg gatcaaaagg gtcaggccgg acgaattttg ggtgcctggg
    10021 cattttccag atcggccgac catgccaggg gtgttgataa tagaggccat ggcgcaggtg
    10081 ggtgcagtgg cgctgttggc taaagacgta gacggtgtta ggaagaaccc caaagagagc
    10141 gcgaaggggc ggcgcctgtt gcccttgttt gcgggagtgg acaaagtcag gttccgccga
    10201 ccggtccgtc cgggagattc actcgctctg gagacggaat tgctgggcac atggggcaat
    10261 ttcggccgtg cgcgttccat cgcacagctg acagacggca gccctgttgc agaggcagaa
    10321 ctcatgtttg cactgggaga tttggatgga ggagatctat aagggtcccg tgctattctg
    10381 cgttctgatg gatgtgccat ttggattttc gcttacaaga ttgatggaga gggcattgcg
    10441 tagcatttgc tgagagccac cgccggttgg gcgagaagag tagagaccag cttgggctcg
    10501 acgcttgttt ggagtgtgaa ttcagattcc cgtgactcgt cgacagagaa gataaggcag
    10561 tttcctattg cgttggcctg ttgtactttt cggacaggct ctggccgcgc aaagatccgc
    10621 atattcgaag agagagtgta tagtctgtcc cctcgaagag gcaccccttt cccgcaaagt
    10681 ctgtgctata ctaaaggctg tgctacagaa agagaggtgc cctattagaa gaattgatcg
    10741 attgtcatat gcactctgta cattcacccg attctgacgc cacgatggac gaaatgtgtg
    10801 aggctgcgat cgacgcagga ctacgcggaa tatgtttcac agaacatgtc gatttggacc
    10861 cgcactattg ctcctatggc agttatgact acgctgaaac tatgaaagat accgcgagat
    10921 gcagagaaaa atacggtgac cgcctgataa tcaagtcggg agtggagatc acctatctga
    10981 ctgagtacgc cgatgatgtc cgacagttcc ttacggagca ccagttcgat tatgtcctcg
    11041 gttcgataca cataatcgac cacgtcttcg taggaagcga agaacatttt atcggcaaga
    11101 ctgagcagga agcctatgag ccttattggc gagagatgat tgccatgatg gaaagcggcc
    11161 tcatcaagag agtaggccac atggattata taaagaatca caggcctaaa gagtatggtc
    11221 ctttcgattt gaaaaaatgg gaggattaca tcaggcatat actccatttg gtcgtggaaa
    11281 acggagctac tctcgaggtc aatacgtcag ccataagaag aggttttccg gaaccctatc
    11341 ccagctggga catactcgag ctctaccggg aggtcggggg aacgcaggtg acgataggat
    11401 cggatgcaca caaacctgac gacattggac agtatttcgc cgatgttgcc aaacgcctag
    11461 atgaaatggg ttttgtcgtt tgtggagaag agatcttctc ctgtagggtc tgacaacagc
    11521 atatgtaccc atagcgccgg cgcagactgt gggcggcacg catatggtgc agtggactct
    11581 atccagggga gtgatctcaa tgccagatga tagggaagag tcaggaatgc gccaggcgcc
    11641 gcagtgccct ggcgggttcc tataccagat cagatctggt gataccctct acgcgcttgc
    11701 gggccgttac gggacaacag tagaagctat aacaagggcc aacccaggta tagaccctaa
    11761 cagcctccgc gttgggcagc ttatctgcat cccggttgcc gcggctccgc ccggaccgcc
    11821 tgcaggggct tgtccagctg gatccacgcc ttatgtaatc cgctcgggag acactttcta
    11881 cagtattgct cggcgcttta acaccaccgt tgctgctctt actgcagcca acccgcgcgt
    11941 aaacccgaac aacctccgtg tcggccagtt ggtatgcatc ccaactgctc cgccgccaac
    12001 tgcggcttgt ccgccgggtt ctacgcccta cgcaattcgc tcgggcgata cgttttacag
    12061 gattgcccaa agggcgggaa ttagtgtaag cgctctagtt gcggccaacc cgggcgtcga
    12121 tcccaataga ctccgcgtgg gccaggtcat atgcattcca ggcgcaccgc ctcctagacg
    12181 tgtttcgtgc acgatgaatc ttctggcgcc agccgggagt cctgcgcccg gtgccacggg
    12241 tagattgtgg atagacacga acgctgcagg caacttgcag ataacagtgg caggcgtgga
    12301 tctccctgct cctgccacct taggagccaa catctacact gcagtatttt ccgcggacgg
    12361 ggtgcggttt tccgttccta tggtcgctac ggtcgagggt cggtggaccg ggactatggt
    12421 tcagccgcct tcaactgcat tgctgacgcg cgggcgcgtg gacatatatc caggccctgt
    12481 tttaagtggc ctattggcaa attgcaggta gaagcctaaa ccagcctgcg atgcgtgaga
    12541 tggtaagagc atcggcccgc ctctcgccct ttgcgccctg agcgcggagg gtgcaggggt
    12601 gggccggcgc gttaaggtca gcatgtggac ggaggagtcg gtatggcgcg agcagaatcc
    12661 ttcggggcaa gcgaagtact gaaagaggtc aggccgcgcg ttgaagcgcg gggatcgaat
    12721 ttggaaagtg gtgagtctgt atgtcgactt cagaatacgg ctttgactac gctgatctgc
    12781 gcgaatcgat ggtggaggag ttctgtgaac tcgtgaggat cgacgcggaa tcaggtcaag
    12841 aggcggagct agccagtgta gtggctgcga aactggagaa cttggggttt gatgtaacgc
    12901 aggatgatgc cgggagcaag ttcgggggaa acgcaggaaa cgttattgga cgttttccag
    12961 gcgtgccagg ccttgagcct attctgcttt ccgcccacct tgacagggtg actccgggca
    13021 agggcattgt gccgtcaatc gacggcgatc gaattgtgtc ggggggagat actgttctgg
    13081 cggcagacga ccttggcgga gtggttgcga ttcttgccgg tataagaatg gcgcgcgcag
    13141 catgcaagag attaccgccg ttggaagtag tatttactgt gagcgaggaa aaaggcctca
    13201 gaggggccag atgcctcgat tactctcaga tacaatcgaa aacgggctac atcttcgatg
    13261 cctcgagccc ggtaggcgtg gttgtcgttc aaagccctac gcacatcagt gttgatgcaa
    13321 cgattacggg tagagcagcc catgcagcgg taaatcccga aggcggcata agcgcgatac
    13381 gcgtggccgc tgaggctatc ggcaagttcc cgtttggacg agtcgatgac cgtacgactg
    13441 cgaacatcgg tataatctcg ggcggaacgg ccacgaacat cgtttgcgag aaggtctcgt
    13501 tcaaaggaga aatacgcagt ttggatacgc agcgcgcact cgatcttgcg aaggaggtag
    13561 cggagcagat aaagactact gccggaggat atgaggcttc ggcggaggtc acaactacgg
    13621 tggactacta cgggtacacc ataccccgcg atgcccaagt ggtgaggcga gtcgaaaagg
    13681 cgttggctat gtcaggccgg gaggctgttt tagattcgac catgggcggc agcgatggga
    13741 acatattcaa caccaagggc atagagagtg ttgtcattgg catgggggcc atggatgttc
    13801 attcgacaaa ggaaagtctg cccatttccg agctggtggc cgccggcgag ctggccagga
    13861 acattatact gctggcgtcg ggcatgcagg atgcatcttt agccgactga gcagctttta
    13921 ttggcgctcg gggcacgttt ttcgggggaa agctcagaag cagcctattg acacgcattg
    13981 gactcgtttt tgtgagttcg ggccggttta tgcattgagc ttgtccacaa aaaggtatcc
    14041 gctggttcgc ctcaatggga tgtgtctcga agttggccag gttcgttgac aacccccgct
    14101 tctcttcgca gcagaccttg gccatcgcca cacacttgtt gtccgggcct caaagggcag
    14161 cggagcattt cagcgctctt tgcgcgactg ttgaatcgcg ctcgccatcc ggcgtaaggg
    14221 cttcagccgc taggatttgg tgagtatcgc ggctatgtta ggcggtgggt gtgcaaggcg
    14281 ggcaagggaa tagccattgc aaacactcac cgccgttgcc tacagatgca gcatatatcc
    14341 gcagctcaga ccttggttgt ttgatccggg caagatactt cttccaagct taggcgctaa
    14401 ccatctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg agaggaccgc
    14521 ggcgcgtatg caacggcacg acagatcttg ctgcggtctg ccataacagg cacgccctgt
    14581 tggaaaagtt taagagcagc cctgtgttgc gaagattccc gccgaactgt ctgatgatcg
    14641 cgtctacgat gaatacggcg cattaacggc cacgcgaaag cagaacatct ccttcactga
    14701 cctagtcccc tactcctttc ttccatgcat cttcatatat ctcggggcca ttcgggcaga
    14761 atccgatcgc tcgggatcat ggaattcacc tgctcattgc atgttttgac tatactgcac
    14821 aacctttggc tctggtgcat tagcccctct cttcaaccgc tttatctctg acccgcccgc
    14881 tatctgccga gtgataacac gaacatgtca atgaatagat ctgatatcgg cgtcaaataa
    14941 cgtggcgggc ggcattgttc tcgcgggggc tgctgatgac cgtggcggcg aagctgttca
    15001 attgcacgcc gcgatcgaga gggatgaaag gcataatgtt caaagccagg ctcctctgtc
    15061 ccggtctgct catgctggta gttatcgccc ttacaattcc gatgtcgttc ttcgagtatg
    15121 tgccatggat ggagggggac atggcatcgc gggtaatgga gccagtattg gtctgcgaag
    15181 agatcatcta caaagagatt ggcgaaatgt cattggggtt cgacatctac agaagcagcg
    15241 agatatcggc cagccctcac atggcgccgg tggtggtata tgtccacggc ggcgcctggg
    15301 tggttgggga caaacggagc ggactccggc ttgcctggcc catgttggaa gcgctctgca
    15361 gcaagggttt cgtcttcgta agcaccgatt accgtcttgc aaacacctac accaaattcc
    15421 ctgcccctgt tgaggacgta agagacaccg tgcgctatct caagaaaaac gccgatcaac
    15481 tcgggatcga ttccgagagg atagctctca tgggcgtatc ggccggcgga caccttgcgc
    15541 tcatggcggc gttggcgccg gacgatagtc tcggaggcga tcccaagcta tgcacgtatt
    15601 ccggcagggt gcgtgctgtt gtatcgtggt gcggtccgac agatttgcgt atcgaacagc
    15661 accgcacgcc tatccgcgaa attctggttg cagagcttgt gggtcaaccc ttccagaacg
    15721 attcgtatct gtatagtgca gcgagtccag cagcgtatgt ctccagcgca agtcccccaa
    15781 ttctcctttt gcattccgag gaggatagcc ttgtccccta tgggcaggcg caatgtatgt
    15841 acgaacaggc catggttgtg ggagccgatg tgatgctgat cagtatcgag aattcgggac
    15901 atgtatacaa gaagatagat gacagggaaa tccagccgga gctcgaagat atactcaagt
    15961 tgattgccga ctacatagaa cggcagctgg ggttagcatt cggcccctgc gagcatcggt
    16021 agcttcaaac ggagcaggca ttctgctgtc agacgaaaag gaattcttcc tggcggcgcg
    16081 agctgccctg tgatacaatg gttttgggta aagccataag gtttggctga gctattgaca
    16141 caactgaata ggtatgcctg cgggtagggt taacgcctca acctagggct ggtgattttt
    16201 gtgtgtgcct gtccgaagct tttggggaat gcttaaaacg ggttcctgaa gctagggggc
    16261 gcgctatcca tctgtgatat ccgatctcaa gcaccaagga tcattagccc gtcctcgcga
    16321 cgggtttttt gattggtgca cacctagcgg agtctggcaa cgagccgctg atcaacgttc
    16381 ccttcccgaa ggtattacct gagaaaacgt cagatcttat ctgacagtcc caaaggaggt
    16441 tcaactgaaa tggcgtgtga aaagcaagat aggagatccg gccagccaga ggccaagaga
    16501 gtgggtgtag ccgccatcat aattcaggac cggctgaatg cggcgcccgc tgtaaacaag
    16561 atcctgagcg aatacggcga gatcatagtg ggacgcatgg gcattcccta tacagacagg
    16621 cacgtctctg tgattgccct catcgttgac ggcgacacag atgcgatcgg ggccatgacc
    16681 gggaaacttg gctcgatccc cggggtcaaa agtcgcgtca cattggtccc ttccgacaac
    16741 gagagtggtg aatgacgcag atttcacaga ggttttgggg atggccgtgt cccatcctga
    16801 agagcttggc ctcaacgagg tgatagccct tttgaaccct ccgggacctg ctcaggagat
    16861 ggcgctgtgg caggcagccc attcggtcag ggtttccgtg atgggcatgt gtgtacacat
    16921 gagggcgata atcgagttct caaattactg ccgccaggat tgcctctatt gcggcctccg
    16981 ccggagcaac aataggattt cgcggtacag gatgagcccg gacgagattg tggaggccgc
    17041 aatagaagct cacagcatcc acccattcgg cacattcgtg ctgcagtcgg gggaggatcc
    17101 gggttatgct gcagatcaac tagcggttgc ggttcgaagg attgcggaaa ctaccggatc
    17161 cgcggttact ctcagcattg ggcagatgcc cgatgaggat taccgggttc tcagggaagc
    17221 gggggccgac cgatttctct taaagttcga gaccagcgat gaacggcttt tccgacaact
    17281 gaagcccacc acctcgttag agcatcgcct tggatgtcta agatccctga ggaggatggg
    17341 gtatcaagta ggatccggca ttatcctcgg ccttcctggg caggatctct cttctgttgc
    17401 ggccgatctc ttgctctgcc gggatgagga ttacgaaatg gtcagcatag ggccgttcat
    17461 cccgcatccc gacactccgc tgggcaatgg ggaaagcggt gtcgccggtg gcagcccaaa
    17521 agcgggcttt gacaccaacg ggacggaggc gtcgacaggg gcggcagtgt tagcctcagt
    17581 gcgaacaacc atagatgccg tagctgtggc ccgtcttctc gttccgcgtg cccatatgcc
    17641 tgccaccacg gctcttgcgg ttatgggcgg gaatgatgct tcgtggaaag agattgcgcc
    17701 atctcctcga ctgacagatg cccggagtct ggcccttgta tctggtgcaa atgtgttgat
    17761 gttggatgtg actccagcga agtatcgcga gttctacacg atctaccctg ggaaatctgg
    17821 agccgatggt gatgtgattt cggatttcgt gaggcaggca aaagaggata ttgacgcttt
    17881 gaatatgacg atttgccctg gcaagggcga cagtccgaaa atgccttggg gaaggggagg
    17941 taaaggtgtt gacaggtaga accgagtgca ccttcataga cgatgagaag atccgcggag
    18001 ccctcgagga aggggcaagg tacagtccag cggcggtcag agatgtcttg gcgaaggcgc
    18061 gagaattgca gggcttggaa cttgaggagc tggcagcctt gctctgggtc gaagacctgg
    18121 ccctcgaaga agagatttac gccgttgcta gaaaaatcaa agagaccatt tatgggaaaa
    18181 gactagtttt tttcgcccct ctgtatgtga gcgactattg tgtgaataat tgcagatact
    18241 gtgcctacaa gagggacaat cggcagccta ggcgaagatt gagtatggat gaggtcagac
    18301 aggaggtcct tgccattgag gacatgggcc acaagcgcat cgctctggag gccggcgagg
    18361 acccggtcaa ttgtcccgtc gattacatca ccgaggtgat ggagaccata tacgacacgc
    18421 attcagggaa cgggagcatt cgccggataa atgtgaacat tgccgctaca acagtggatg
    18481 actaccggaa gctgaaggac gccggaatcg ggacttacgt actgttccag gagacgtacc
    18541 accgcgatac gtatgtggct gtccatgatg gaccaaagtc cgactatgat tggcacgcta
    18601 cggccatgga cagggcgata gaaggcggaa tcgatgacgt gggaataggc gtgctctacg
    18661 gcttgtatga ctggcgctac gagaccgtcg ccatgcttgc tcatgcccac catcttgatg
    18721 agacatacgg ggttggaccg cataccattt cattcccgcg actgaggcct gcagcaggcg
    18781 tgtcgctcga gaattttcca agcctcgtct ccgataacga cttcaagaag atagtggcgt
    18841 gcctgaggct ggctgtgcca tatacaggga tgatcctgtc gacaagggag tcgccggagt
    18901 tccgcgagga agtgatagct ctggggatct cgcagataag cgccggttct tgcactggcg
    18961 taggcgagta tagcaggcgc aaggcgcatg atactcccca gttcgaggtg agcgaccgtc
    19021 gtgatctgga tgagataatc agggaccttt tgccgtcagg ttacgtaccc agtttctgca
    19081 ctgcatgtta caggtcgggt aggacggggg aggcgttcat gtctctggcg aaaagcagcc
    19141 acatacatga attctgccag cccaacgcaa tactgacgtt tgaggaatac ttgtgcgatt
    19201 atgcatcgcc tgctactgtg aaagcggctg agcctgcgat ccgcgcggcc ctggcagaag
    19261 tggatccgaa gctacgcgac gaatgtgaaa agagaattga gcggatacaa ggtggcgaaa
    19321 gagatctcta cttctaaata aatctcgcta tatgcagcaa cgcgggagtg annnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcctcaagc gttcgcgcat gcacatcagt
    19501 ctagcgccgc agaggcggac tggaggacgg aaatgagttt cgacaaggct cccagatcca
    19561 atcggataca cattgctatc ctcgggcgaa tgaactcggg caagtcgagc cttattaaca
    19621 ccttagcaga tcaggacgtt gccgtggttt cgccgatggc cggaacgact acggatatcg
    19681 tctacaagtc gatggagata catgggaccg gcccggtcgt gttcatcgat acccccggag
    19741 tagatgatga cagccttctc tcagaagcga gggttgccag gaccatgagg gcgatcaaca
    19801 aggccgatat ggccgtagtt gtggtcgatg ccaagttggg cgaaggggag cctgaggccc
    19861 agctatattc ctgtctcgag cgcaagaggg ttccctatat tgtggccgtg aataagacgg
    19921 acctttgtga tgccgactgc gttttatgtg acggtgcggg cgcctccggg gaagaggatg
    19981 cgcctcgtgt gtgtgtaagt gctaggacgg gaaggggaaa agaggaactg ctaggtgcca
    20041 taagccggtt ggctcaatcc gtaggcggac tgggcgaggg gccccccata gtaggcgatc
    20101 ttgtaagctg tggagacatg gtggttctgg tcattccggc agatgcccaa gccccgaagg
    20161 gcaggttgat tctgcctcag gtccaaacca taagagatgt tttagatcac gaggggattg
    20221 cggtcgccgt aagaattgac caattgaggc agctcttcgc cgataaggat ttcctgccga
    20281 gattggttgt gactgattcc caagccttcg gggaggtagc ccccgtagtc ccggaatccg
    20341 ttcccatgac atctttttcg attctctttg cacgccataa gggtgacttc gaacttctgg
    20401 tcgaaggcgc tcgcgccata gaaggccttg agcctggcga tgtagtgctc atatccgagg
    20461 cgtgtactca tcatcccatc gaagacgaca tagggactgt gaagatccca cgccttctca
    20521 atgcgaaagt cggcggagag ctgagatatg aatggcaacg cggaggcgat tttccggagg
    20581 atttggacag attcaagctt gtcattaact gtggcgggtg tatgcttaac aggagagaga
    20641 tgtgttcgcg ccttgaggcc gtggcagacg ccgctgttcc agtcacaaac tatggaatgg
    20701 caatcgcagc gtgtctgggg atcttgccca gggccttgcg gcctttcgca ctctgaaatg
    20761 aaccgtctgc ataagcccgg tgatccgcga gtacctcgag gcctaaggag cagagatagc
    20821 ggactgtgcg gccgatcccc aagtcgaatc tttattgccc gatacttgaa ttaggtgtgt
    20881 cgacgcccca actgcattgg tcataatgct tcagccttgc ccacaccatg cccaaaaagg
    20941 tgagacaaca aggcgaggga cgtcgacact tcacattatc taagggactt ttctgcagac
    21001 gggaggtgta tttggccgac aaggtcggct ccaagacgca aggctccggc ttggtcctgc
    21061 gataccaagc cggagcttta catcggaaga agagagttga acctcccgcc aaccgcattc
    21121 tcttccttga taggctgacc gttgtaggaa tataatagtt atacttagcc gtgaacaacc
    21181 atccggaggt gggcgttttg gcgattagtc caagactgct tgagatacgg agttgccttg
    21241 tgcatgacta cgaaccgttg atagattacg gtgcatggcg tgtggccgct atatgctcct
    21301 ccgagcgcaa cgcagttgac ggggtgaagt cccttgaaag gcataacgag acagacgaag
    21361 tctttttgct cttggcaggc agggcctttc tcattatagg cgagggagac gcaagtgtcg
    21421 aaacgtttca tgctgtggcg atggagccgc tcaaggcata caacgtgaag cgaggcgtct
    21481 ggcattcgtg tgtgctcgac acagattctg tagtgcttgt tgttgagaat cgcgatacta
    21541 cccacctgaa ctcggagttt ctcgatcttc cgtccgaaat atgccatcaa ttgcccgaca
    21601 tctgcggtga gatagtttag agcctcggca tgttccgcgt caggctgccg ctttgccagt
    21661 tatgcaaatc tcaagccaca tcggaaaaac ctgagccggt gaacgccaat aacctcaaaa
    21721 gacagaggag tttcggacat ctagtgcgaa acttacatgc agaagcatat cctcatcctg
    21781 cctgtgctgg tagctcgcga cctattccca aactaagtca tgcgggcgac tccgagccga
    21841 ttcttatcat gatcaatctc ttggttttta tagatcgagc gtgattacaa aatgaattca
    21901 agcgtcgagt cacaccctta cacctccttc tttgctgatt ctcgggaggg ccgttaagtt
    21961 agctgaaaag aagatggtga cttggaaatg atgtatcatt ttgtgccaat gggctgtaat
    22021 tgtcgagatg aggtgatagt caatgtccag aagaagaata gcaatcgtcc ttgtatgttt
    22081 tttcattctg tcgctcgggc ttgtagtggg aagcccggca tactgcggcg aaaccttggc
    22141 aagcaccaca tattatcagg gcggcaagag atttcaagtc gatgttgacc tgggggcaaa
    22201 ttgggagatg accctcaccg tctaccggat tgagctgagc ggaaaaccgc gcaagttgtg
    22261 gagttatacg ggtgaccaca tagaattgga aatggtgcgg gatgttgacg gggatgggtt
    22321 cgttgaagtg ttggcgatgg agtacggcgg gaatacctac gcctgcccga tcctgttccg
    22381 ggtggataag aacgagaagg tgcggcagat accgatcgat ctagggaaga tgtatgagaa
    22441 accgaattac atgttgatta ccagagcagc ctcgttcata gatctggatg atgatggagt
    22501 ggatgagctt atcgcctggg ttccacagta ttggatgccg tattacgcca atagcgatat
    22561 ggtttatgcg tcgatcatat gcaaagcgaa ggacaaccgt tatgtggcgg ctgccggcga
    22621 ttatgcttca gtataccggt tcctcataag cgaattgaga aatgaaatag ttacatacgg
    22681 aactgacatc ttggaactag acgtcgggcc atacctccaa aattgctgtt tgctgttgct
    22741 ctatcgatcc cttgttggcg agatgacgca gggactccag gaattcgata ccctgtttcc
    22801 acatgcattg aaggtcatcg accctgttgc tgacaagtgg tttgccgata catggaagga
    22861 cttcatgaag cgcagagtgg atctgctgac gcaggcaacc agggatttcg gggggattgc
    22921 gcagaaacgc tgaattagtg cgggccgatt ggagcatgaa cgagcagccc cactactggg
    22981 attgcatggt aatggcgcca accgacacct gacgaatggc caactattca ttgaaggtat
    23041 attgctgacg aagatggaag tatcacacgc gtgtctgttg cggaaggact aatgcctaaa
    23101 gcccaggttg tgtgaccggg gaggtgctca aagcgaatgg agcggagcag gatatatggc
    23161 gctttcgagc gcgtgagtag aaaatggtgg ttctacgtct tggtcgttat ggccctgggg
    23221 attccgcctt atgcatccac gggagtagcc cctgaacaga tagccagttt agttcaagaa
    23281 gttctttctc aggggttgac ggcatatagc gctctcatgc ccatgctgca cattgctgtt
    23341 gcagctctgg tcgtgttggt gggcatttac ggaaacaggg tgggaagact cttcagcgct
    23401 tttatggggc ttcactatat attcatagcc attgcgcaga acactgccgt aactcctact
    23461 ttcggatatg ccgttatcag cagcaatgtc cttttgttca tggtaatcgg gataatgtgg
    23521 ttgtgggatg cagcgatcgg gaagaccgac tacacgctgc gagggccgca tccgtggtgg
    23581 acgtattggg ttctgcctct ggcagtcttg gcgttttggg gacctgatgt cccgtggagt
    23641 ttccatcttc ggtacctagt gacgtccagt tctgtcggag cgttctgcct gatgacgcct
    23701 atctaccttt ctgtcttgag cttctgtcct aggtataatc ttcctgcggt tcgacttaca
    23761 agctttgtcg gaatactcat ggccatattc aatttcatga taggcttgtc tctttgcata
    23821 ggcggcaatg caagcctcgg gatataccag gtattcatac atctgccgct ggcggcactg
    23881 tcgacatatt ctttcataag ctcgtttcgg gcgggtcgcg cgaggcaacc atgttcgtag
    23941 cgaggatcca attgatcttc agaatgagcg tgggggagcc ctcgagttaa ttcgttaaga
    24001 ggtggaggga attacgaagt ggagcctttt gcgtctcttg accatgagag gtacatgcgg
    24061 gaggccctat ctgaggcaga aaaggccttt gagcggggcg acagaccgat aggcgccgtt
    24121 attgtgcatg acggtgagat aatcgccaga ggttctaata ggttcttcag cgagagcagc
    24181 tatattgccc atgccgagct taacgcatta gtatcagcag caccctacct gtatgaccat
    24241 ggacgagagt gcgtcattta caccactgtt gagccatgcg taatgtgtct tggcgctatt
    24301 atacatgcca acatcaggaa tattgcgttc gggatgcacg acaactacat ccaagcccgt
    24361 aagatcatcg atgcggttga atacgtagac aggagagtcc ataattacct cggcggcata
    24421 ctcgaaaagg aatgtgtgga gctttacagg cgctactccg aaagggaagc acagctaatg
    24481 ctgacgggaa cgcagaactc ctggtaggtt tgaaaagtgc tcagaggagg gatagctaga
    24541 ttcgtcagac aaatggatat tgacaggtga gcgccatggg ggcaaagact ccagatagcg
    24601 tgaatcgcga aaacgtgcag gaatctgcat catcggcagc ctgggttttg agtagactcc
    24661 gatcgcgcga gacggaggcc gtttctggca gccggttact gcggaggctc gccccttgga
    24721 ggttcgtcct gcacacctgc taagcgactt caaattggtt cttgcgagag tcttatgagt
    24781 aagaccatgc ggcaaggctg ttttacgaga gcccatcgac gcatgaaatg tgaggttggc
    24841 tgatctcgct cgtgagaagg atttgctgtt cgtgactggt aaagctgaag tcgtgtacat
    24901 tctgactggc cagggttatt cattggtgtt gagcgttgaa acccaaggcc agttcctgtc
    24961 tgcgctaggc aagttatgat atttggaggg ggattggctt atcatgcgtc gaaaagctcg
    25021 agaacgagac gattctctat gtgaaacgca gtacgagggg ttcttttcag agttctatga
    25081 tttgctgcac tcatcctaca atgctgatct gcccacatac ttggacttgg ctgaggaata
    25141 tggcgatccc attttagagc taggatccgg aaccgggaga ctgttgattc ctcttgccag
    25201 ggctggatac agggtgaccg gactcgattg tgctgaagac atgttcgcac tctgcaggaa
    25261 gaagcttgag gcggaaaacg gggctacacg ggaacgagtg actcttgtaa atggagacat
    25321 gagggatttc gatctcgaca tgaagttcaa catggttttg gcggcgtgca atacgatact
    25381 tcactgcacc accattgatg atcttctggc tgtgcttgcc agagcccgtc ggcatctaag
    25441 ccaaaaagga gtcttcgtag tcgattttgc catacctaat atcctcagga tgcttcagac
    25501 aaatggcgcc gaggccaggt ttgaaatggt ccatcctgtg agcggcactc gcataatcga
    25561 tacatatgtc gcaggctacg atttcaccaa acagatggag acgtacgagg cgcgcattga
    25621 ggaatgggac gaaagcacga tgctgagatg gagcgaggtc aagactgaac gggccttcta
    25681 ttttccccgc gagatagagc ttgccctcag gtgcagtggt tttgagatag tcaaaacatg
    25741 gaaaggtatg cgcggaggtc cgttggtgga gaccagtcaa gacatggttt acgtgtgcag
    25801 attggctcgg cagggttagt cgagaggatc tagccatgtt acaggcgcca gaggccaagc
    25861 atgtttagat tcatgattcg tctatcgtga ttgtctggag caacgaagcc gctttgggta
    25921 gcgactcgag atcgaattct gcacacgcga catgcagagt gatacagcag ggttcgatgc
    25981 tgatgccttc cagatcgagc gaacggaagg cgccctcgta tatctggagc agatcgatga
    26041 ggatgagccc ccgtccgagg tagtcaacgc cgggcggcag aacgggcatg atagaactga
    26101 ggactatgtc gggaaggctg agtcctagtg gcccggagat tctgagggcg acgacggccc
    26161 cttggaccgt cgggttcgag aggcagatta ccggatggaa ctttatattg tacggcagtg
    26221 ccaactggag cgtaatcatg cccgatgcga tttttacatc gactaccttc agcccttgag
    26281 gcagctccat gttttgcagg gcgtgtttga tttctgcatc gcatattctc actgtcaagg
    26341 attccactct cattcatgtc tcacccaccc ttccagaagc ccctcggcca ctcattctta
    26401 taggcgtcga ttggggagta tgcgatagca gacgattcag gcaactggga gcgaagcttg
    26461 caattgcttg gcgaatccgg tcgaactttc acattaggtt acagaattct tcgatactgc
    26521 gtgtgcttcc tcctttattc cattcctgca ggaagtatct tagctgattg ctccgtcagc
    26581 aggccacctc ggaatggctt atgccattct atgcttgcag gagtggaagc catttaggcg
    26641 aatctgcaga atcaagccgg tgatgtgcgc gatgacggga gggatcatgg tgaagagaat
    26701 tgccaaggcg atactgattg tcgtagccgt ggctttgctc gcactcgtcg cgttcatagt
    26761 ggttatagcc gccacagact acagaccttg tgacattgag ccagttgaag tcgaaaaccg
    26821 ccgtgaatgg gtgaccgaat cgggtgcaga gttgagcgtg accacgttca acataggcta
    26881 ttgcggactg gacaagcaca ccgacttttt catggacaaa ggcacgatgt cgcggtctat
    26941 aagccgggag cagacagaga tcaacgtcga cagagtctca cagttccttc aggaggccga
    27001 ttccgatttc taccttgtgc aggaagtaga ccggaaggga aagaggagct acaatatcga
    27061 tcaatacaag agattcattc gcgactttcc cgaatacagc agcagtttcg ctacgaatta
    27121 caaagttgca tgggtgccga tccccgtcac ccaccagtac ggcaacatct actccgggat
    27181 tgccacatac tccacgcgcc gggtagactc tgccgaaaga ttctcattgc ccgggcagta
    27241 tccttggctg aaacaactct ttatgcttga tcgttgtctt ttggaaagca ggcttcctgt
    27301 cgataacggg aaggaattag tcctcgtcaa tttgcacctg tccgcatatg atcagggcgg
    27361 gaccataaga gcccagcagc tcgcgttcgt aaaggagtac gctgtgcgag agtggggcaa
    27421 aggaaactac gtgattttag ggggagactg gaaccaccta ctatcggcca ctcccaagga
    27481 gaagaaggcg tcatacggag atgcctggcc tgattggctc cagcttttgc ccgacgattt
    27541 tgtcctggag gggttcgagt gggccatgga tgaggaagtc gccagcaaca gatgtgtgga
    27601 tcgaccctac aacccggcta ccacggttgt gcagacgatc gacggttttc ttgtgtcgcc
    27661 caatgtggag attgtagccg tgcaaggcat tgatttgggg tttgaaaact cagatcataa
    27721 ccctgttact gccgtattta agctgaaata acagatcgtt gggagcgcat gttgtgtaaa
    27781 actgcatcga ggggcttggt tgccccgtcc cgtgccttgc aagcttcgcc tgctctgacg
    27841 gctgtgttga agcggatgtt atgggtgtct ggttgacgca ttctttcgaa cacccccgag
    27901 ggcacgtgca tgtcccattt tccagatgat atcaccaagg cgaatctgga gaagccgaaa
    27961 gccgccggag caacaatggc cagaggtcct tcaacagagg ctgcgcaggc cctcgtcgat
    28021 cattgcagaa ggacagggag ggaaaggact tctgttcggg catttgcaga gcccggcaca
    28081 aagaggcact tgttgttgtt caatatggtt tttggggctt cggcatatct gccgctacgt
    28141 tgctctggtt cgagttctga gtgccaaata aatgtggttt gatttgtagg gagaatggcc
    28201 aatatcggct gatagatgag acggctcatt cagtgcagtg cctggtttca gctaagaagt
    28261 attgcacatt agcaaatttg gagtgattct gtatgaaaag agtgctcaac aaggtcaaag
    28321 tggcaacagt ggcggcggtt cttgcgccac ttttatttgg actcttctgc ccctattcca
    28381 cggttgcagc aacacagatc tccgaaaaat ggataggaaa atcatctccg gatttcgtgc
    28441 tgagaacatt ggacagcaag aagattgcac tcggcgatga cccgaaatcc gtctatgtgc
    28501 tggatttctg ggctgtaggg tgcggaccct gtaagaggct cgcccctcac cttcagactt
    28561 tacattcaaa gtatgcacca aagggagtta cggtagtagg ggtaaacgtg ggggacagtc
    28621 ctagcgcgat aagcaagtac gcaaaagaga acaagctcac ttatacgatg gcaattggag
    28681 atgcgaggac ggtggatgca tttggagtct acgcatttcc cacagtcgtc atcattgacc
    28741 gcaagggcgt aatcagagac gtgtatgtag gatacctccc tggctgggag cagaagatgg
    28801 agaacactat caaacagata ttgggggact agcgctgtgg cggagagatc tggagctggt
    28861 ccaacccgcc aagttccagt atgggctttg attctgacgg cgggcgtggg gctgatggcg
    28921 gttggcgcaa gactgggcga cttcgagatg atatttctga acgcaaaact aatctgcctt
    28981 tcgtgcatag ggattgagtg atagatggct atacgaaaac ggaaatcgtc ggcccgttcc
    29041 atcgtgcagg cgctggcgac gattgctgca aattcaggct tcccacagat gaagatctgc
    29101 ccgtaccccg ggctgaactg ttatgcctgc cccttggcat cctttgcgtg tcctattggg
    29161 tcgcttcaga agttcatcgc cacaaggcag ttcccttatg ccatgaccgg gttttttgcg
    29221 acggtaggag ctgtagtggg gcgggctgct tgcgggtggg tatgcccctt tggatttctc
    29281 caggatctcc tggcaaaatt ggggccgaag cggaaacttc ttcgatctca gcgagggaat
    29341 tggatcagat acgcggtctt gatcgccttg gtcggagtgg gaacatacgc agtcgggcag
    29401 ccgctgtttt gcaagatatg tccggcgggg acactagaag gcggaatacc gcaggtgctt
    29461 acgaaacccg cgctaagagt gctcatcgga ccgcgctact gggtgaagat cggccttttg
    29521 gctgtgatga tcgcggcttc gatattgatc aaaaggccgt tctgccgatt tgcatgtccg
    29581 ttgggggcca tctggtcgcc atttaacaag gcgagcttga tgcgcttgga ggtggacgag
    29641 ggagcttgca ctcagtgtgg agcatgcaag aacgtttgcc ctgttgaact tgaggtttac
    29701 cgagatccga attcggacgc gtgcatcagg tgcctggagt gcacaaggtg tgatgcggtg
    29761 agcgtgagct ggagccggaa gtctaccagc gcaggcaaag cttgcgttgg cggtctgtag
    29821 gggatcccgc acgcaatcga gtgcttcgca tcttctagga ttggcaagac gctctggagg
    29881 aggagtggct gtccgtcatg gaggcgtgcg gatggcgttt cacggagcgg ggctttttac
    29941 acattcatca gctttgagca aaacaatgca gtctatagaa ccgagcatag agcgctatcg
    30001 aagcccgacg cagacgagta tttcggtctc ttccaggatg caggctggga gcacgtggcg
    30061 tcctattcga atacccacta tttcaggtgt ctgcctgagg catgcgagtt cccggaaatc
    30121 cattctgata gggattagtt gcgggacaag tatcggcgca gggactggat aatgctggct
    30181 tttattgtga tatacttgcc tgattggatc accattgtct taagaccggt cccatcaggg
    30241 aaaggcccat ttctgaccgg gctctattcg ttaggaagat ggggttgcgt cctcttgatc
    30301 gccatggccg tacaggatgt acatgctgat gaggtcggac taggcgactt aggttcgtta
    30361 gcgtctcgag caatcctgat gatcatattc ctgtttggct cctatgcatg gggtgtcccg
    30421 tgcacggaat cagacttgga cacctatgtg gtgctgcctg agaacgcaga catgagagag
    30481 attgatgcgg tgaagttgat tcgcaaagcc attcatgaca aaaagacaat gcccgtggat
    30541 gtgatagtaa gcaaagagaa cgagtctaac cgctggaaaa ttgcccccac tattgaacgg
    30601 tagtttgtgt aggagagaat agtgctgtat ggatgacagc acagtctagg gactgataag
    30661 tcagtcagtc gaagaaagcg tcgacgaaat catcgaggct atcatcgaag aaagcagatc
    30721 agagcttgtt ggcgatacca ggttatggaa cactgcgaat aattcgcagt gttttggcat
    30781 gacactacga atatttcgcg gatagcatta cagttagaga cagttaatgg aaggtaatac
    30841 ctttcggggc gccgaatccc catatttatc cacacagcag gccagtacaa tagcatgaat
    30901 aggtaatatc cgggtcggta tggaaaatac aaaccatata aagtcattcc aattcatatt
    30961 atgtaaactt cacttctaac gctacgattt tttcgtagca taagaaaaaa tactacgaca
    31021 aaatcgcact gtcgcccttg tcttgtccga ttcccgccta atgacgaccg acagggatca
    31081 tttcatgcgc gcaggcaccg atttggatag gtgctattcg gtacttgtcc tcgttttcaa
    31141 agagcgccac acaactggcc gggttttgtt cagcaaacca aaaactaaag atgatcttga
    31201 tcccaatgcc atatggccac ctacagccgt tccattggca actcgagcta tttcggtttt
    31261 ggcattctgc tctgttcaca agataagtgc ctcgagctac cgttgtcaat caccatcatc
    31321 cacagcaaag acgcgtgttt gacgctggga taagtgaata agatgtgccg gcagatacgt
    31381 tatggagttg tggtccacaa tttcgtaagg cgcaggcagc ttcagccatc cggtctcacg
    31441 acttctgtgg tagtatcagg tggtgggttt cacttaggtc attaataatc atcgagggtc
    31501 ttagtgaaat ctgtgggtag ggatgatggg ctttcccctg ctcaaccact gtagggaggg
    31561 aaatagattg gctaagggat tgaaggatcc ggttagcggg ctgactcatc ttgcaggtgc
    31621 gatcatgtct gttataggcc ttgttgtact gttgcgagca ggtattgcca atgaagatct
    31681 gtggcagatt gtgtcgtttt caatattcgg tgccagcctg atattgcttt actccgccag
    31741 cgctgcatat catttgctca atgtcccgtc cagtgtggaa cttgtattgc gcaagctcga
    31801 tcacataatg atcttcgttc tcatagccgg cacatacact ccggtctgtc tcggtccgct
    31861 gcgtggcgct acgggctatg tcatactgac gataatctgg gctttggcta ttgccggcgt
    31921 gttcttcaag gtattttatc tcaacgctcc gaggtggttg tataccggca tatacttggc
    31981 aatgggctgg cttataatct ttgctacagg ccctttggtc cgtgcagtag gccttcacat
    32041 tctcagttgg ctccttatcg ggggagtctt ctactctatc ggggcagtga tctacgggct
    32101 caagaagccg gatccgttcc cgggccggtt cggctttcat gagatattcc acatcttcat
    32161 cctcctcggc agcctgagtc attttcgttt tatccatttg cttgcgtagc gtatctttct
    32221 tgctgccttg gtcttctctt tttttctgaa ggccaagttg cgggtattat ggcaattagg
    32281 ttacaagctg tatttctgct atgattagtt gagatgagtc tttcctcatt tacacatcgg
    32341 gaggtggccg tatgaggttc gctgatctta ttgccacagc tgtgcggcgg tgtcgcgcaa
    32401 ggctcctgga gtcgttcctc attgtgctgg gcattgctct cggagttgca gtggtggctg
    32461 ctttttcagg actcatcggc acagtaggaa aagagtaccg ccagttcagt gattccatag
    32521 cattcagaga gatctgtatc gagtcaacgt cagggcgaag cggtggaagc tactccactg
    32581 ataggccggc gtttgccctg tcgcggactg gcgaggcgcc gtacgaacta tctctacatg
    32641 atgtggatcg catcaagtct agggtttctc ctgacgtgta cgtctacgca tatgaggact
    32701 tttttcaaca tctgcggcct ggggacgatc tctcggtcgg ttcatccctg gacgattcta
    32761 ttccgaaatc ttcagaaaac ccggaaaagg gccctgtgcc gcgaaatgtt cgagtgattg
    32821 ctgccacgga agaggtgttt tccgcctacg gcgccgaatt ccgggaaggc atagggttct
    32881 cccgccctga tgtagcttcc ggcgaaaggt gcatggttct cggcagcagg ctggctgaga
    32941 ggttctttgg agatgcacct gctgtaggga agaagatgat atggcagaac gaatggatgg
    33001 atggccccac aggtgaactt gagctttcgc cttccgggca tgtctatact gtgatcggag
    33061 ttatggaacg gattgagccc ccggtttcag ctgggaaggc aggcgaatct ccagatgaca
    33121 ttgatttctc catcgatgga agtgcctata ttcccataac ttcagcccct ggcatggagg
    33181 gccccaatcc gaaagtccac gagatccatg ctgtatcagc cttatccaaa gatgctgcac
    33241 gagctatgga tgaggtgagg caggcgatag agacggaata cgatgggcgt cttgacgcga
    33301 gctcggggat ggaatacatg aaagagagta tgcgcgagct gcgaagcttt gccactggca
    33361 ttctggtttt ggcttcggcg ggattggtca tagcatcgat caacattctc aacctaatgc
    33421 ttgcacgggt tttgcggcgt actcgggaga tcggtatttc ggctgccctg ggcgcaggcc
    33481 ggcgtgagat attccgagag agtctggcgg agtcgctggt gctcgggata tcaggaggcg
    33541 taataggcct tccgctctct tacggcctgg taaagctgat gtcccgcatt atggaggggc
    33601 tcgatgtgtc cgtcggcctt acaggggttt tagccggggc tttgtcctgc atcgtagtaa
    33661 gcgtgatttt cggactctat ccagcctacc tcgctgcctg tataatcccg gcggacgccc
    33721 tgagggggga ttaggccgat gacttcgcat ctgttcaaat ccgcgtggat caatatgagt
    33781 cgacgcccaa tgcagtctat tctgacgatc attcaagtgg ctcttgcagt agcctgcatg
    33841 gtttcggtgg caagctacag gatgaatgtg tcgacagcgg tcgaccgttt caagtcttgt
    33901 actgatgaca tagtgattgc catgggcggg atggaaaagc gcactgaggg cgggtatatg
    33961 agaaacatgt acaatatttt cgatgacgac gacattgccc aactcaatgc tgaagtttcc
    34021 ctcgtggagt ctgtaactgt ttacgaggat atgtgggaca ttgaggctga tgtggatgga
    34081 acgttgtatc gaattggctt aggtgcgtta gtcgggcctg gctatcaaga cgtggtcggc
    34141 ttcgacatga tcgaaggcaa cttcataacc gatgtggata tgaaggcgca gtcccgcgtg
    34201 gtcgttatct cggaagccct cgcgaagatt ctgtttgggg agggccccta cgtaggcaga
    34261 acgcttggca ttatgccagt ctgggcgcga cggagttttg ctaatgaaga cggggccatg
    34321 cccgtggcac ccactgaata ccatgttata ggcgtataca gcaattctaa gacgtctacg
    34381 tcagcgatgt cgagctatac tggcatgcat ccagccatcc tctgtccgct cacgtccgat
    34441 cccaatcaga tttacagttc aggccagggc tggttatgca tggacgaaga gtatccatac
    34501 ggcacattga tgatcaaggc cgcccccggc aaagctgcag cagttcgtga ccgtatcgtt
    34561 gccatggtgt caggccgtcc gagtcccgat tcattgagcg gaagtagtgg gagtatggtg
    34621 gtcatgggag gcggaagcgc tgaatttgac gggccagggt cagatggcga aggctccgat
    34681 tggtattcgc cccctgatga agagagcggc gcgtcggtga tattcgagag aactgatgac
    34741 ttggagcaga tgataggctc atctatgact aagatgacgt tgttgctcgg cggcgctacg
    34801 cttattgcga taatcgtgag cgctctgggc attcagagtg ctatgatggt aaatgtgact
    34861 gaacgcaccc gcgagatcgg cttaaggcgc gttcttggcg cctcccgccg cgttgtggtg
    34921 gtccagttca ccgttgacgc catgatactc gcggcagtag gaggcggatt cggagggctt
    34981 ctgtcatttg ccctgtatcc atacctaatg aattctgtct tttccagtat gggcccggga
    35041 tggcttttgg acgtgagtgc gaggcctgtg ggatgggcca tattagccgg gattgttctt
    35101 gccgccgcag tgggtgccct ctttggggcg atacctgcgg cacaggctgc ccgtgttcag
    35161 cctgcagata tagtgagaga gctttgattc ggggaggtat tcttatggct gcaggtacgc
    35221 cggaaaccga aagggagtat gagcccatag tcaccagtcc ggcgcggact tcatcgagca
    35281 agacccgccg cgacatgggc atgctcatat tcatcatagc cgtgttggtt ggcgcggttg
    35341 ccctggctgc acggtttctc atacccaaag aaaaacccta cctcctctcg aactacacat
    35401 ttgcccatgt cagcacggct gatttcactg actctgtgag cgcccccgga accgtagcgc
    35461 cggctaccac agtggacgtg cgtgctgacg tttctggggt ggttgccaag ctcagcgccg
    35521 ctttaggtga tgaggtgaat gtgggcgacc ccatatgcgt tcttcagtcg ccagagcttg
    35581 ttacaagacg tgacgaggca gtgcgccagg tggttgctgc tgaggatgca cttgccaagg
    35641 caaagctcga tgctgctcaa tcggaggaac gggtgaggag agagatcgtc gtagccgagc
    35701 aggcggtttc agatgcactt aatcgacgtg aggatatgga gaagctgttc gaggcgggcg
    35761 ctgtgtcccg agtccaggtg gacgaggcgc gggccgcgga aagcaaagcg cgtatggcgc
    35821 tggataacgc gcggttggat cacgatgcca ctattaggtc gaacgccctc tcgatcgaca
    35881 cggccacaag gcaggttggc gtggcacgga atgctttgcg cgctgccgag gattgtattt
    35941 cacagctcac agtctgctcg cctattaaag gcaaggtgtt ggaactgagt gtcgttttag
    36001 gcgcaacagc gagccaaggg ggagtggtcg cccaggtggc ggatatggag agcatagtgg
    36061 taaaggccaa agttgcctcc acagatgtcc gccgggtgac ggtagggcag cctgcttcga
    36121 taaacctggg aggcagaaag gtggctggag tagttcgcca tgtggcgccc aaggccgaag
    36181 cgagcggaca gggagcgact gttgaagtga caatcgattt tgatgaacgg ccgggcgacg
    36241 tgcccccaaa cagtgctgca ttcgtcgata tagaggtgca gagacgtaca gatgtgccgt
    36301 ccttgccccg cggcgcttac ctggccagca gcagagaact gttcgtatac gtaatcgaag
    36361 gttccaaagc ggtccaacgg gacgtgagat tcggttcaat cttcggcaac cgaatcgaag
    36421 tagtagacgg gctgtcggtg ggcgagagcg tgatcacctc atcctatgag gagttcaagg
    36481 ataggcggga gataaaagtt gcgcctgagg gaggacggga aagccaatga acgaggatag
    36541 acaggctcag gctttcatac agcttgagga cgtgtgcaaa gtatacgcga tgggggatgt
    36601 cagcgttgcg gcgctgggag gaatttcact cgaaatcccc cgtggggagt atgtagcgat
    36661 aatgggaccg tccggttcgg ggaagtccac tctgctcaac atcatgggcg gtttggacgt
    36721 ggcaacatca ggtaaatatc tgctagaggg cgtcgaggta acgcgtctgc ccgatgctga
    36781 gcttgccagg atcaggtgcc gtcacttcgg attcatcttt caaagttaca acctctttcc
    36841 ggaactcaca gcaatcgaaa acgtgatagt ccccatgatg tacgcaggcg ttgcgccggc
    36901 tgatcggacg caacgggctg cagagctcct tgattcgctg gggatgggcc accggcttcg
    36961 ccatctccca agtcagcttt caggcggaga acagcagcgc gttgcgattg caagggcgct
    37021 tgccaacgac cccgacgtga tattcgccga cgagcctact ggaaacctgg ccacagcgca
    37081 gggcgaggag atattggata ttcttgaagg gctcaacaaa agaggggtta ccatagtgat
    37141 agtgactcac agcgagaacg ttgccgaccg cgcagaacga ctcataacca tgcgcgatgg
    37201 gcgagtagat tcagaccaga cggcggctcg ggtgtaggtc gggcgcagca ggccttgcct
    37261 gactgacaac ctctcatcat ttgacccgca actcaaaaca gagctcaaat cggtctgcgt
    37321 aacgtcgtct ctttagtcgg ccggactgcc agtctctcct gagatccgca aatggcgccc
    37381 attctgccag cggccataca tattctcccc gaaaagagga ttgcagatat ccaacggttg
    37441 cttttgtcac aatgagagtc ccacagcaag tgtggataag ccactgcgcg attctcttta
    37501 gttcagatct tggccgcctg ctcagatcaa gatgctcttc gcaagttgcg cagaagcaca
    37561 agatgggggg agggactttt tcctattgca taatctcccc tgaggtgcta tcatctaacg
    37621 taatatacaa atacacacgt taaagcgatg atcgggaaga gtaggcgcgg acctttcggg
    37681 cagcgagccg gggacggtgc gagcccggtc cgaaagagcg actgaaccac acccgggagt
    37741 gcgggctgaa cgcgggcgcg aaagcgttct gctattaggt tccgacggag tctttcaccg
    37801 ttacaggaaa ggggatataa ggcagcgctg cttctgtcgc ctgttgcgcc gctgtccgta
    37861 tccttgatga gtgggttcga ggcgccagct tcgggctaat tagggtggga ccacgggaat
    37921 atcatgcttc tcgtcccttt gcgggacggg gagcattttt tctttagtgc gcactgtgtc
    37981 cgcccagtcc aacttgtctt cgcaatgata tcgtcgacaa aacagcagac aggagcgtga
    38041 tcgattgtgg acaactttcc gacttgggga agatactatc tccccgctga tgccctcatc
    38101 agacgacata ttgagagcag tctggcgtgg acgttcgaag cccggggcta cgaagagata
    38161 gtcccgccgc tgctggtggg gttggatatg gctgtactcg actcgagtgg ttcgaccttt
    38221 accaatggcc tggctggggc agagaagcca tacgcagtcc ttgcaaggga tggcagcttc
    38281 tttgccctgc ggtctgacat gactgtccct gttgcagctc tggcagtgcg ccacctcttg
    38341 ccatcggaaa gtcctgtccg cctctattac tcaggcagcg tctttcgccc ccagaaggcg
    38401 ggaaatggaa aaccggagga gcggcgccag tttggcgctg agctgatcgg gacagacgct
    38461 gcagacagcg tcaaagccga ttgtgagatt cttgccatgg ctgccgaagg gctaatcaac
    38521 acaggagtgc gggacttttg catcggcctg agcgacgcta agctcttctt ggccatgctc
    38581 gatgcagcac gggtcgagga tgatgcgaaa gatgccatct ggtcagcgct ggcatcccga
    38641 gactatgtcc gctatggcga gctcgtatcg tccctcgatg acggcggcga tcctcagaaa
    38701 gccctcggaa tcgatcttct caagcgcttg cttgccctag agtcaacaga ggagtcgctc
    38761 gatgcgctca ggagggcagg ggtctgcgat gcagcttccg agcttatcag gatacttgat
    38821 gtcttggaag agcggggttt gggcaagtgc tttcgtctcg acctgtccat agtccgggaa
    38881 tttcggtact acaccggagt cgtgttcgag ggatatgccg ttgggtgcgc aaatgccgtg
    38941 atcggcggtg gacggtacga caaactgctg gcgcgatttg ggtcgtctgc gcccgcagcg
    39001 ggtttggctg tggatgtgga cgccgtggtg tcggctttgc aagcagtaga aggaggtgag
    39061 cgtcgatgga acaggtatct gtggcccaac acgcgcccgt gcgcgaaagt gaggtcgcgc
    39121 cactaaatct agggaactgt ctcagcatcg cgcttcccaa aggccgattg ctccagccat
    39181 cgctggatgc cctggcggaa gcgggacttc aagttccaag catggatgag atagacagaa
    39241 gactcatcta cacgacgggt gacggcagta tgcggttcat tcttgcccgc cctgcggatg
    39301 tgccagcata tgtcgaacat ggcgctgccg acctagggat aaccggaaag gatgcgttgc
    39361 tcgaaggcga ttggggcgtt tgcgaactcg tcgacttagg ttttgggaga tgccgcttgg
    39421 ttgttgcagc gcttcagggg gcgattgccc cagatggctg cggctctgag ggggccgtct
    39481 gcaagctgcc tttgccgcgt agtggccaca ctctacgggt agccaccaag tttccggcca
    39541 ttgccagacg ccactttgcg ccgaaacggg atctatgcgt cgagatcatc aagctcaatg
    39601 gggctgtgga gattgcgcct attgcggggc tttccgacgt aatcgtggac atagtggaca
    39661 ccggccgtac attggccgac aacgggcttg tgccaataga ggaggtagcg cagttatcag
    39721 cgaggctcat tgcgaatcgc gcgagcctca agctgaaatc cgcagtcatc catcagattg
    39781 cgtcggcgct ttgtgatcga agccgcagca tctgacggac ttaggcatcg agcagacaga
    39841 tggattgctg taaagctgag gcgaggttta tggccattaa aacatgccaa tgccaggagg
    39901 tgttgaggcg aagatggata cgccaatcgg gataaagact attgcagctt gcgactactt
    39961 gcagcaacga atccgtcgag ggcctgagga cttcgaagag gttgagcaga aggttcgccc
    40021 catcattgaa gacgtccgaa gacgcggtga ccaggctgtg ctcgactaca ccgcgcagtt
    40081 cgacggtgcg gattttgtgc cagaaagcat tgaaactacg agcgccgaca ttgatcacgc
    40141 ctactctgtg gtggatcagg gcgttgtaga ggctctccgg aaggcggccg acaatattcg
    40201 gtgtttccac gagcggcagg tacagcagac ttgggcgtgc attctggatg acggcgccat
    40261 catggggcag gcaatccgac caatagaacg ggttggcatc tacgcgcctg gcggcgccgc
    40321 ggcctacccg tcctctgtgc tcatgtgcgc ggtacctgcc cggatagccg gggtgcagag
    40381 cgtgattgtc tgcactccac ctggtcccgg tggcgttgcc aacccctatg tgctcgtggc
    40441 ggctgacatc gttggcgtcg acaaggtgtt caaggttggg ggatgtcagg ccatcgcggc
    40501 gatggctttt ggcaccgagt ccgtttgtgc agtagacaaa atcgtgggac cgggcaatgc
    40561 ttatgtgact gcagccaaaa ggttggtctt tggctatgtg gacatcgata tgctcgccgg
    40621 accgagcgaa cttgtcgtgc tagcagacat gtccgccgac ccgcgcttcg tggcggcgga
    40681 catgctttct caggctgagc acggtcctga ctcggaagcc atattggtga caaccagagg
    40741 cgaggttgcc gcgagtgttg tccgcgaaat agccgttcag gttgagtccc tgtctcgtcg
    40801 cgatacgatt atacgttctt taagcgaaca cggccttatc ataatcgccg acgatctcga
    40861 ccaggctgta gacgtggtaa accgctatgc ccctgaacac cttgaagtga tggtggacga
    40921 tccccatgcc cttctcagtc ggctgcgcaa cgccggcagc atactgctag gtccgctcac
    40981 gccggtggcg gccaccgact acgccgccgg accgaaccat gtattgccca caggtggcac
    41041 agctcgcagt ggatcgcctc tttcagttcg ggattttgtg aagatacaga acatcacatc
    41101 gcttacgcgg gttgcactag acgatatgat ggatgccgcc gagaccatcg ctgcagtcga
    41161 agggctggat gctcacgcag ctgcccttgg ggcaaggagg gtaggattat gatagcctca
    41221 gttgccggga gaggaggcgc caatgaacgg gatttgggtt cggtggccga gttcgccagg
    41281 cctgaacttc aagggcttcg agcttgcgtg cctgattggc atggcgtcag agtcaaactt
    41341 gacagcaacg aaagcccctt cgatctccct gcatgcataa aggaaaatgc cctgaggaag
    41401 ttgcgggaga tcccgttcaa taggtaccct caagggctgg cggcgaagct cagggagacg
    41461 attgcgttcc gcgacggggt cagcgagaca caggtgaccc tcggcaacgg gctggacgag
    41521 gtaatctaca ttatcctgca ggcttttgga ccaggcagga aagtaataat ccagaagccc
    41581 tcttttacta cgtataccta tgcggctctt tccgctagcg ctgatatccg ggcagttccc
    41641 ctcgacgagg gactgcactt gagcacagcg gggcttatcg aagaggcgaa tgctgccgag
    41701 tcgccttgta tcttgatcat atgcaaccct cacaacccta ccggagggcg gtttatggag
    41761 gaagacatcg tggatgtgct cgaagctacg aattccatgg tcatagtcga cgaggcatac
    41821 gtggagtttt cgggcggaag cgttctcccg ttggttgctg agtatgatcg actgatcgtg
    41881 ctgcggacgt tgtcaaaggc tttcgggttg gccggggtgc gtgtcgggta ctccgtgtcg
    41941 agctctgaga tcgctgccga gttggaccgg gtcaggcagc cgttcaacct cgactcgctt
    42001 gccttggttg ttgcccagac cgctctggaa aacgacagct attgccggga agcggtcatc
    42061 cgcattgtcg aagagcgcga gcggcttttg ggccgccttg gagaaattca cggagtgacc
    42121 tgtttcccgt cttgtacgaa tttcattctg tttaggacaa cgatgccaga tgcaacgatg
    42181 gtcagagatg ctcttcgtcg gtatggcgta tctgttcgcg tgtttcccga tgagcccatg
    42241 cttcgcgggt gcctgagggt gagctgtggg acaaaggaag aggacgacgc attcctggag
    42301 gctttggaga aggtgttggc agaagcaaac caaacgtccg ccggtcttcg tgacgacgac
    42361 ggaaaaagga ttgagggtgg tgagtaggat gcccaaaccg agactggccg aagtgcaccg
    42421 caagacatcg gagacatcag tcgagctgag gttgatcctc gacgctgcca gagaccatgg
    42481 ctgctctggc cgtcatcagg cgaacatctc aacttgctgc ggattctttg atcacatgct
    42541 caggctgctg gcatttcacg cccagtttag cttggatctt catgccacgg gagacatcga
    42601 agtcgactat caccatcttg tggaagatgt gggcatctgt atcggcgagg cattctgcga
    42661 agcgctaggc gacaagcaag gcattgcgcg ctttggcagc gcccttgtgc caatggatga
    42721 cgctttgagc atggccgtcg tggatctgtc ggggcgtcca ttccttcatt tcaatgtgcc
    42781 catgccatgc gcgaaggttg gcgatttcga tacagagctg gtggaagagt tcatgcgggc
    42841 tttggcaagt aacgggaagt tcacattgca tgtagacctg ctccacgggt ggaacacgca
    42901 ccatatcata gaatctgtct tcaaggcggt ggctcttgca ttgaggcagg ccgcggcagc
    42961 aggagacacg tcttcgattc cgtccacgaa aggggtgttg taggatgcta gtgcttccgg
    43021 ctatagacat ccacaacgga aagtgtgttc gcctgtttca gggagacttt gcgaaagtca
    43081 ccgagtattc cgatgacccg tgccaaactg cccggcgatg ggcgaacatg ggcgctcaga
    43141 tgctgcacgt ggtcgattta gatggagccc ggcaaggtat gccggtaaac ttggaggttg
    43201 tgcgcgacat catagcccac actggtcttc ctgtacaagt gggcggaggt ttcagaactc
    43261 cgaaggacgt cgaatcggcc cttgaggcta aagcggcgcg agtcatccta gggactgcgg
    43321 cctgtagcga cccggcaatg ctccgcgacc ttgttaggag atttggcgag gataggatcg
    43381 tggtgagtat agattccaac tgcggtgcag ttatgacaga tggatgggtg cgagcctccg
    43441 gcatcgctcc ttcagaactt gtcgagcgcg ccctggactc tgggatccag acagtcatct
    43501 atactgatgt gtctcgagac ggcactttgg ccggggttaa tgttaattcc atcgctcagc
    43561 tgttgtcggc aggcgcaaac gttatagttg caggcggagt cagttcgatc caggatcttc
    43621 gtcagctgaa aggcctggaa agccagggag tgagcggagt catcatcggc cgcgctctgt
    43681 acactggcgc gatcagattc cgagatgcgt tgagagcggc aggaagcagg agaatcattc
    43741 cttgccttga cacgaaggac ggccgcgtgg tgaagggggt taacttcgag aatctgaggg
    43801 atgcaggcga ccccgttgga ttggctgaga tctatgagag ccagggagct gacgagctga
    43861 tgttgctcga tctgtctgct actgctgagg gacgcaggac tgccctggat cttgtgggcc
    43921 gggtcgcgtc tgccgtctcg attccccttt cagcgggcgg gggcatcaca agcctcgatg
    43981 atgtcgggcg agttctcgat gccggcgcgt caaaggtgtg catcaactct gcagcggtgc
    44041 gaaaccctca gttgctccag catgcggcga aggccttcgg ggttgaccgg atcgtctccg
    44101 ccatagatgc atccgccatt gcggagccat ttttggatgc aggcaacaga catggcgatc
    44161 gtgatgtcaa tggcatcgta agcatcgagg ttgacagcaa gagcgatggt aatggtgacg
    44221 gctgtggcag gtgggttgtg tgcacattcg ggggcaagca acgcacggat ctcgatttga
    44281 ttgaatgggc tcgcaccgtt gagcggctgg gggcaggcga aatcctcctc acgtctgtgg
    44341 atagagacgg gtcgacggac ggctacgact tgcggcagct gcgggccgtg actcaagcgg
    44401 tgggcattcc tgtgatcgcg tcgggcggtg caggaacacc tgagcatttc cgcgatgcgt
    44461 tcgtggaagg cggagctgat gctgcccttg cagcctcggt cttccacttc ggaacgctga
    44521 gcgtgggaga tgtaaaaaga cacctcaagc gggaaggagt tgacgtcagg ttatgattga
    44581 acgattggaa tcggatgatg gcgaaagcct ggaaggatgg atagacacgc tcaaattcgg
    44641 tgcagatgga ctgattcccg ctgtcattca ggagagtgtg actggccagg tgctgatggt
    44701 cggatacatg aatcgccagt ccatcgaaat gacgctccgt gaaggccgga catgtttttg
    44761 gagtaggagc cgccagaggc tgtggaccaa gggagagact tccggacatt tccagaaagt
    44821 gcgggcggtc ttctgcgact gcgaccgcga ctgcttgctc atagaggttg accagacggg
    44881 cgaggcttgt cacttgggcc gaaagtcctg tttctttacg cagttatggg gcagtgcaga
    44941 tgctgctggg accgggttca gtctggataa gctctacgaa acagtggagg atagaaaggc
    45001 aagccaaccg gaaggttcgt acacggccaa gttgatccgc tccgggcaag accgagtgct
    45061 gaaaaaagtg gctgaggagg ccggcgaagt gatgctggct tccaagaacg acgacagaga
    45121 ggagatcatc agggagatgg cggacttgtg gttccattcc atcgtcgttt tggcatacca
    45181 cgatattcct ccgcaggcga tcctggacga gttggccgat cgtcacagta atgcgtggaa
    45241 agcggaaagc ttgtgcacag aaagaaaaga gggccctgga tactgaggca agagcgtccg
    45301 cgggccctgc acatggacgg gaaagcttag atgttgtcgg agcctacgga ctgcaatagc
    45361 ctggactttg ccatgaagca tgcagccagt tgataggcga gatcggactc ggctttgcgc
    45421 actgcgtcta tagcttctga gacttctgca tacgcgactg cgccaatacg gcattgctcc
    45481 tctcttatgt ccaatgttgc cgtggcgatg gcgacagcca aacgcgccag ctcgaccgat
    45541 gactctgctt cgcgaagatt gtagaatgct tcaactactt caaggagtat ggaatccctt
    45601 gtttcgacgg catttgcttt agcctgttcc actcggcctt ttgcggactc gatggcgatc
    45661 tctgcagcgt aatccctagt aagcagtgcg gagacctcta ggcccacgcg ccagtgtccc
    45721 ccggatgacg cttgcccttg gaaaaagaca tccgggcgtt tggccgcaat cgcttgagcg
    45781 agggcagcct cggcatctgc gacatcttgc tctgccatgg gaatctcggg cctcgtttcg
    45841 agcgccgccc gcgtacaggt gtcgagatcc aggtcttcag gccatctcac atactctccg
    45901 gccggcgcga ggtgcactag agtatccagg tctctgccta tagcacggtt aaagctgatg
    45961 gaagcaatag ctagattgtg ccgggcagat tgagctgccg cttcggccct gtcggcgccg
    46021 gcctttgctt ctagtgcatc aagttcgctt gcgttccccg cagaacatcg cccctccacg
    46081 aaagccgcat gagtgcgggc acgcgagaga ccgcggtcag cggcggcgac tcggtcttcc
    46141 gctgcaagga gattgaagta tgcggcctgc gctgaagcac gcgcttggcg tatcgactgg
    46201 tcgagctgga actgagcact ccgcaattga gactgggcag agcttgttga gcttggggcc
    46261 tcgcccgggg agctagcgcc gagtatgggc agggacaggc ttgcactgcc gttccaggcg
    46321 cttttgcccg ataaggccgg gtctgaaaat gcaaaggatg cggtggccga caatgaaagg
    46381 ctcccgccac ccagcggaat ccgcacgtct ccgccagctt gcgaaagcgc atacatggac
    46441 gtctcgcctt ttcccatcgg tttgctgtct tcttcgacga cactctggac acttagcggc
    46501 ccaatcccta tttgcagagg tgcggcatgg accgttatag aaggtcgctt ttgagcttct
    46561 gcagcctgga gctgaagtat tgcaagctcc acagcgcgct tggccgtagc gaccgatggc
    46621 gaggaggatt cggccaggcg cattacatct tccatggtca gagccagact ctcgtcggcg
    46681 tgtgatgcgc ctagagaaga atccgcagca aaggtgcatg gtgcaagggc gaggagtaac
    46741 atcacaacgc agatacagat gtctgcagct ctacggctga acggaaccgt gacaaaagcc
    46801 gtctgccgct gctggctttt gtgttgagca gtctggctca tcggggatca ccaggctcag
    46861 cctcttcggc cggatagttt gcccaggcat caaagtgcag tcgggcgatg ttgtagctca
    46921 ttatcgattg aagctcggag agcctggcct cggtgaggct caactgcgct cccaagagtg
    46981 aattggcgat ttccatgccg tgctcgtaac ggagcttcat tattctgtag ttttcctctg
    47041 cctgtttggt cgcggctgag gcgattgaaa tcgccctgtg ggattcctca agcgacagat
    47101 acatctgccg ggcttcaact cgcactccgc gctcagcctg gcctgcggcg agcctggcct
    47161 gtgccagagc attattgagc tgccgcttgg tcagttcggg agtgtattcg ttatctgcga
    47221 acttcgcttc gagctcgcag atctccaggg tttctcgggc cttgacgatc tccgggcgat
    47281 tcttcaaagc aagggcgagt gcggcttcgt agtcaacatc tccggcaatt ggctgggctg
    47341 aatcgatcgc aatgacaggt gtgtcaaggt ccatgcctaa ggtgcgcttt agggccagtt
    47401 gtgccagttc aagatggttt ttggcagaca acgtcgaggt tactgcctgc tccagagcga
    47461 tttcagctga catgacatcc atcttgcttg ccaggccggc ttcgaatcta agacgaacga
    47521 ttcgcaggct ttccctggcc tgttcttcgg aggcatgtat tatgttggca cgctcttgcg
    47581 ctgtcacaag attgtagtag gcctcgtcta catcgagcat gagggattgt tcttttgcga
    47641 caagggcacg tctagcgtcc cgtctggcgc cggctgcgcg tttggaggcg atcacggatg
    47701 gccttatgag ggcatctgcc tctgccatct ggaactggat ttcggaagct tcgaactcga
    47761 gcactgccat ggcataatca tagctgtggt caatcgcgta gctgcgcgct tcatccaggg
    47821 tcaattccaa gggctcaggc tgagctactg cgcatagccc gccggatccg gagagggcga
    47881 gaagcgccag aacggccatg gttacggcca gaggtgactg tcggaaatgc cgggcgtgag
    47941 cgggattttt caacctcaaa cttttggaaa gcggtttgat tgtcattatt ccaaagtcct
    48001 cctaacggga atcacttggg tctgataata ggtagttcgc cgtacggccg gttcctcctt
    48061 cggaggcaca tgaagggtgt atgtgcgaga ggcaggtagg ctttgggctg ttttatccgt
    48121 tcaaatcgat gatggatgca ggcggcacag acgtgattca tctgcaggat gtgagcatac
    48181 gggaaatgga actgactgag aaaccttgca aaggaaattg actacggtgg agcgccagct
    48241 taccccatag ggtgatcgct ggctgtccgc cgggagccgc cataagccaa cgcatgagca
    48301 cgcagcaact atatgatagc ctgtggctgt tgcctcgctc actctgatgc aagttccgcc
    48361 aatgccgtgg ctgccctgtc gacgtcagcg atggtattga accatccgaa actgaaccgg
    48421 accgtgcctt cagggaaggt gcccacagtc ttgtgcgcta ggggagcgca gtgaagtccc
    48481 gaccttacca tcactccaaa ttccgcatcg agctcgtagg cgagtcttcc cgggtcgatg
    48541 cacccgtctt caaagctgat tgacacggtg gcaactgcgt catcggcaga cgcctcaccc
    48601 gagccccacg gtccgagaac gcggactttc ggcccaatgt cgaccgcctg ttgaacgaat
    48661 cgactggcca attcggcttc gtgacgcctt atgcggtcag gaccggtttt ttgaacgaat
    48721 tgcatgcccg cggccagccc ggctattccg acggtattcg gagttcccgg ttcgaactta
    48781 tccggtagaa atccgggttg tctctcttca tcagacttgc ttccggtgcc tccctgagcg
    48841 agtgggagca tttccgcggc tgccctgtcg ctcacgatca ttccacctgt tcctggcggg
    48901 ccgtatagtg ccttgtggcc tgtgaaagcc aagatgtcgg caccgagggc cccgaaatcg
    48961 aggtcgataa cgccggcggt ttgcgctgca tccacaacga agaaagctcc tgcttcggcc
    49021 gcgattcgac ctacatcggc aactggcaag attgtcccga cgacgttgga agctccagtc
    49081 atcactatca tctttgtgtt cggtctgatc gctttcctga tatcatctgg gtcaagtctg
    49141 cctagggaat cgcacccgac tatcgatact tcgacgcggt tttgatcctt tgcatggcaa
    49201 agaggcctga ttaccgagtt atgctccatg ctggtcgtta ccacgtggtc ttcagggcgg
    49261 agcaagccgt ggatggcgca gtttagggcg tatgttacgt tgggggtgaa gacgacgtgc
    49321 tcctctgaag gggcgcgaaa gagccttgcc gccgcaatgc gcgcattcag agtcagacgc
    49381 cctgcatcaa ggctgaggga gtagcctccc cgcccggggc ttgctccgat gttgagcatg
    49441 tactgttgca tcgcttccca tacctgaggc ggcttgggga acgaagttgc agcgttgtcc
    49501 agataaatct ttgccatggt tgaagacttg atggagcgac aatcgtctat attcaactgt
    49561 agtccctttc tttctgcatc atgtgtcgtt ttcgcgtcat ttccaattcg tccgcagtcg
    49621 attctcttgg atgcaagagc ctctccaaga cgaaatctcg gaaggccaga gcagtaggtg
    49681 ataggacttt tcgcttgtgg ttgatcatgt aaagctgccg atggaggtcg atgcccttta
    49741 tgcgcacggc tttcagcttt ctttccgcta tctccgcagc cgcggcccgc gctgacacga
    49801 tgccaagccc caatcccgcg tctgctgcaa ctgccgcctt gattgcctct gcgctttcaa
    49861 ggcgggctct gatattgagt ccgcgggggt ctatgccgtg ctcctgcagt gccttatcga
    49921 atgtgacctg ggtgccagac cccggctcgc ggcagacgat gggttcggca agcaacgatt
    49981 ctatgtcaat tatatctccc caagccgcgt atttgctttt tggaggagcg atcaatatca
    50041 cttcgtcttc tatgatggga aatgattcta tgctagcctc gttgccaatt gatccgacga
    50101 tgcctagatc tgcatcgtaa ttgagaattc tgtcccacac ctcttgcgaa tccgcatggc
    50161 gcaactggat gaagactttc gggtactcgg ccaagaattc ggccacgaac tctgggatca
    50221 cataccctgc tggaacactg ctggccagca atgttagggt tcccgagaag tccgacttgt
    50281 aatggtcgat ggcttccgct gctcgatcgc gcatggcgag gatatcggcg gcgtaaggct
    50341 tcagcacctg acctgcagga gtaagtgtca ctccatggct tttcctttcg aaaagtgtga
    50401 cccctagttc gcgctcgagt gctgcaatct gcacgctgat ggtcggctgc gacatgaaaa
    50461 gggatactgc ggcctcggta aaggaaccgt ggttggcgac ggcaagaaag atagagagtt
    50521 gctttatgtc catgatgtca attcactctc cggcttcaag gtaagcatgg ccaacggcaa
    50581 tcgttgacgg cagggaaaaa cacgcgtccc atcgcgggcc gcttgccccc ttatgcaagt
    50641 atctcgcggc acaccgccat tgtcaacatg atattagata gcgtaattca attgatacga
    50701 attattaata gtctgtattg tgaacgcctg gagatggtca tatacttcaa ttggctccac
    50761 attgtgcgag actacatttt ctccatttta tagttggagt ccatgttcta agggaggtca
    50821 ggtcgtcgtt atgaaccaac gtcgtgtcgt aatagttgcc ggcgctatcg tgggagcgct
    50881 cagtatcatt ctggtggcaa cgggcaatcc gggcaatatg gggatatgtg ttgcgtgctt
    50941 tatacgtgac atcgcgggag ctctgggatt gcaccgcgct ccgatagttc agtatattcg
    51001 ccccgagatt ccgggcttca tcatcggatc gatgatcgct gcaatggctg caggggagtt
    51061 caagaccagg ggcggatcat ctccgcttct ccggtttgtt ctcggctttt ttgtaatgat
    51121 agcgtcattg gtattcttgg gatgcccgct caggatggtt ttgcgcctgg caggcggcga
    51181 cctcaatgcg cttttgggca ttgcgggttt tgcattcggc atatggatag gaacactctt
    51241 cctcaagcgc ggctttactt tgggaagaag cacagcgatg cataagggaa acggggctgt
    51301 aatgccgttg atcagcattg cgctgatcgc gcttttagtg gccgctcctg cgttcatctt
    51361 cttcagtgag accggtcctg gctcgatgca cgcaccgctc atactggcgc tgcttgctgg
    51421 aatcatcgtt ggggtggtag cacagcgttc gcgcctctgc ttggcagggg gaatcagaga
    51481 cttgatattg atacgcgatc cacacctatt caatggtttt gtggccatat tcgcagtagc
    51541 cgcactcctc aacctcctca caggcaggtt ccagttcgga tttgacggac agcctgtggc
    51601 ccatactgat ggcctgtgga atttcctcag catgtcagtg gtgggactcg gtgcagtgct
    51661 cctcggcggg tgcccgcttc gtcaatgcat actctctggc gaaggtgacg gagatgctgc
    51721 gattgcattc ctcgggatgc ttgtgggggc ggcatttgcg cacaacttcg gcctggcgtc
    51781 ttcgccagcg ggggtgaccg gcgcaggtaa ggctgctacc ttgatcggac ttgcggttat
    51841 gctggcaatc ggttattttg gggcagtaaa cgctgttgac gcattgagta cagtcggtgg
    51901 cggcaagaag actgctggcg gcagcagata ggggggatgg accatggcaa cacaagtaga
    51961 cgctaggggg ctttcttgtc ctcaacctgt agttcttact aagaaggcta tggattcgat
    52021 cacatcagga gaaatcgaag tgctcgtgga caaccctgct gccagggaga atgtcgcaag
    52081 atttgcgagg aatgctggat gttcggtgga gattggtgag gagagggatg gctcgtatgt
    52141 catttcgatt tccaaggcag gacgtaagag ctaagcagaa taacgttgag ccgccccatg
    52201 aggctgaatc ttatgagagc agggccggtg cctccggccc tgccagttgc gttgcgggat
    52261 gttcgtttgg ggtcggaaga ggagtcgagg gagcgccgaa aagggaaaag gaagcggctg
    52321 agtcggcaga agaagtggag ctgcttgtta cattccccag tgtacatcat gcgctcaaat
    52381 tggagcggat gatcaaaaca tgtgcgtgtg ggcaggacgg ctcgggacgg tcagtcgatc
    52441 tgatgcctgt tccacgccgg ataagttcca gctgtggtct ggctgccagc gtttgcgtga
    52501 ccatgggcgc ggggagtacg tccgggaatg gtgttgcccg attgggcagc ccgggtactg
    52561 tttgccgttc tttggtggag gagttgaagg cggctggcgt agaataccag ggcatatacc
    52621 tgcctctgcc tggcggcgag tttcagcttc tgaccgatgg tgaagaggcg gggatgtagc
    52681 gaacccggga tagaccaggc ggacggcgag atgagatgat agtttcgccg tttattctga
    52741 tttacctaaa aaccgtccca ggcccaatat gggaagtcct ttgcctggcc tgcgctgcga
    52801 agttcacctt gcttgcagcg tttcatctgg ccagctggtg cgggcattaa tagattgatg
    52861 ttgctttaaa tgccaacttg cacccatctc ctataaatcc agtctgattt gacgcatgta
    52921 cacatgcgtg ctcaaccaat tcatctcccg tttcactcaa gttggtttgc ccatgcagtt
    52981 ggacgcgttt tcgaatatgg gagcgagagc tggtgaatgt taggggagcg cggatgcgcc
    53041 cagtctcccc gattgcctac tctggaggtt ctgattttcc caaatcgctg ctgagctgag
    53101 ctcggaaagt ggcgagcaaa ccacggaatt tgaagcaaac acgggcaggg aaatcattga
    53161 ggtctgagga atagtaacaa gaacaatata tggaactgcg cattgaaatt ggaggtggct
    53221 ccgggttgaa taggagacca ttgcgttcgg aggttccggt agaggaaacc tggagattgg
    53281 aagacgtgtt cggatcggat gaggaatggg aggccgcgct gaaatcgttg gacgccggga
    53341 ttccggctgt tgcccaatac atgggaaggc ttaaggatgg accgaggctg atggcggagt
    53401 gctttgaggc tgcagaaaac ctgatcagac gtgctgcgcc agttgggctc tacgcttacc
    53461 tccgtcttgc ggaagacggt tcaaatccgg ccaatcaagc gatgatgggc cgttcagcgg
    53521 cgatgcaggc ccagatcggg gcagcgctag catttattga gcccgaggtt ctgagcctgc
    53581 cggacgggac cgtagagcga tatcttaaag aggagccgag gttgcacccg ttccggcgcc
    53641 gtctcgagcg tatccttgac gacaagcctt acgttctgac ctttgagacc gaagcggccc
    53701 ttgcaatgtt gggcgaggtc atgaacgccc cgcagatgct gtacgagcgg gccaagagct
    53761 ctgacatgca cttcgattcc gtagttgatt ctgaaggaaa agagattccg gtttcgttcg
    53821 ccacctacga ggtgatgctc gagaagtcgc cagatgtcac gctcaggcgg aatgcctacg
    53881 cttcgtttac cgaaggattg aagaaatacc gtaacgtgct gggaggcaca tttgggacgg
    53941 agatcaaaaa gaacgttgtc atggcgaaga tgagagggtt cgattcggct actcacatgt
    54001 ttttgcacca gcaagaggtg ccgatcgatg tgtatgagaa tctgctcaat attatccaag
    54061 aggaatgtgc gccgcacatg cggagatacg tggggcttcg caagtctgcg ctaggcctcg
    54121 ataaaatact ctattgcgac atagaagcac cactcgaccc cgagttcgac cctgagatca
    54181 gctacgaaga aggtgcgagg ctcattatcg atgctgtcca ggtaatgggg gctgaatacg
    54241 cggagatcgt gcagatggcc ctcgaggaca ggtgggtaga tagggtagac aatgtaggca
    54301 aatcgacggg ggctttctgc gcgcctgctt acgacagcca cccctacgtc cttactactt
    54361 ggggtgggca catgcgttcg gtctttactc tcgctcacga gcttgggcac gcggtccaca
    54421 gctctctcac agctcgcaac cagaggcttg tcaatacgga agtatctagg gtttttgttg
    54481 agtcgccttc cacgttcaat gaaatgctgc tggcgcgcca catattgagc aagacggatg
    54541 acaaaaggat gcgccgctat gtcttgatgc agcttttagg gacctattat cacgatttcg
    54601 tccgccatat gctcgaggca gagctgctta ggcgcgtata tgcccttgcc gaaaagggcc
    54661 aacctattac cgcctcggtc ctttccgagc tgcagggcga gattctggat ggttactggc
    54721 agggactggt cgaggtagac gaaggggccc gcctcacttg gatgcgccag ccccactact
    54781 acatggggtt gtacccctac acgtactcgg cagggcttgc ctgttccacc gctgttgcgc
    54841 aggctctcga tgaggggaat actcaagaag tggcaggccg ttgggtgcat gtgctgaagt
    54901 cgggaggcac ccaaaaaccg ctcgatctga tgaggacggc aggggtcgac cttgaggatg
    54961 caggtactat caggggcgtt gtagattatg ttggaagcat cgtggatcaa gtggaggaga
    55021 ctatgtagac atttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgct
    55141 catgaccacg ttggaagcgg aggtgtgcat atggctgaaa gactggcgcg ttcggaggtg
    55201 cccgtcgatg agacatggag gctcgatgac atgtttcaat cgatggaagc atgggaagcc
    55261 gagctggcag cggttgagcg gattattcca caagtcgcag agttccaggg gagactgggg
    55321 gatggcgcaa gcgttctccg aaagtgttac gagacggtgg agttgcttgc aaggcggttt
    55381 gagaagatct ctacctatgc ctacttgcga ttctcggaag atgggacgaa tcctgtaggc
    55441 caggaactga tgggacgaac gatggcagcg caagctaggc tcgattctgc gatcgcgttc
    55501 atgcgccccg aaatactcag tctgccggat ggaaccctcg aaggctattt ctcggaagaa
    55561 ccaggccttg agacgttcag aaggcgtctg gagctaatcc tctccgacag gccatatgtt
    55621 atgtcagctg aaaccgagac tgccatcgca gcacttaggg aggttctcga cgctccaaag
    55681 atggtgtacg aatggacaaa gacgtcggac atctcatttg aacctgccgt agacagcgag
    55741 ggacatgagg ttcctgtctc ctttgcaacg tatgaagaga tactcgaggt ctcgcccgat
    55801 gctgcgttga ggcgcagcgc gtatacttct ttctcgcgcg gtctggcgcg ctatcagaat
    55861 gccttggccg gcacgttcgc agcagaggta aagaagaatg tggcgctggc gaggatcagg
    55921 ggctacgagt cggcaaccca tatgtttctg catcagcagg aggtgccggt ggaggtatac
    55981 cacaatctgc tcgatgtgat tgaggaaggt cttgcgccgc atatgagacg gtatgtagac
    56041 cttcgaaggc gcgtgctggg gctggacaag ataatgtact gcgatatcga ggcacccctt
    56101 gaccctgact tcatgcctga gatcagctat gaagagggag ctaggctcat catagattct
    56161 gttgcggtca tgggagcgga gtatgcggat atcattcggg tcgctctgga ggacaggtgg
    56221 atcgatcgtg cggataatat cggcaagtcg accggggctt tctgtacgtc tacgtacgac
    56281 gtgcaccctt atatccttgc aacatggggc gagagcatgc gttcagtctt caccatcgct
    56341 catgagcttg gccatgcagt gcagtcggtt ttggcaagtt caaaccagcg gttcgtaaac
    56401 gtcgaaccgt ccctggtttt catagaagcg ccatcgacct tgaatgaact gcttctcgcg
    56461 gagcacatcc tggagagtgc cgatgacttt cgaatgcgcc gttgggtgct tatgcagctt
    56521 ctagggactt attatcacaa cttcgtccgc catctgttgg aagcggagct tgtaaagcgc
    56581 atctacgcgc attccgaggc gggtcggccg ataacggcag cgttgttatc agcggaaaag
    56641 ggcgatattc tggagtgcta ctgggatggg gttgtagaga tagacgatag tgcaaagctg
    56701 acatggatgc ggcagcccca ctattacgcc ggtctttact cgtacactta ttctgcaggt
    56761 ctgacctgcg caactgttgt ggcgcagtct gtgcgcgagg agggctcgcg agctgctgaa
    56821 agatgggtga atgtgctgaa acaagggggc tcaatggcac ctttggaatt gatgaaaacg
    56881 gccggcgtcg atctggagga cgcggctact attggcgatg cagtggacta tgtggggcga
    56941 attgtggacg aggtggacag aggttttgga ggacagcctc actgagccgc tcagcttcgt
    57001 caaggccatg acgggatagg gggcggatat tgaagaagga agcgccgcga cggtgcagaa
    57061 accggtcgcg ggctccctag ctattagcta ttgctggact tgctttcggg ctcgccgtct
    57121 gactcgcaag cagagtccgc tggctcgtgg tgaccactgt aggctggaat agacgaaagc
    57181 gaattgagat actcgtcccg ctgaattggg gtgaggtatt tttccatgag gcgaacgtcg
    57241 cgaatctccg gttctaactc gatgaggagc tcgacgggaa cactcgccca tattcttctt
    57301 ttgcctgccg taatagggag aatattcgaa atagactcac atgtagtcgg cgaagggatg
    57361 actctgcagg cgtctgctga aggcgagtcg agtttgccat catcattctt gctccagttg
    57421 aacaatgctg ccctggggct tgcggcctca acttcgagtc cttcttcgac cttcgcctca
    57481 ctggacgaaa agtcaattcg gagcatgcaa gtttcatcaa atcccgcttc cacaaagaga
    57541 cgggggaggt tgtacacgcg ataagtttgc acataatcga tttcaagttc aacgaggtgt
    57601 tccgacctgt cgcatatgtc tgacacagat gacaggacag aagggttggc gcgaacagcg
    57661 gtcactatct tcccctgcct aggatccacg cagatgaact cctctggcgc ttccgactct
    57721 tctttgggcg tcattgctgt gcccgcaggg atcagttcgt gctctgagcc gtcagccaaa
    57781 agaagggata tgtcagagac cagcctgggt atgcagttgc gataggccat gatcgctgcc
    57841 ccttcggcga tcagggttga ggaagcggaa ggcctcacta tgcgatccgg ggtcatcagg
    57901 cgtccaaagc cctctacaac cgggggcaga tttgtgcttt ctccgatgag caggcagaga
    57961 tctacgtcgg atggcttggc gcctgccgat gcgagcatct cttctgtcgc ctgaaggaca
    58021 cggtcgacaa tgggcctgat caaatcctca aactcggctt tggagaaaga ctcttcgata
    58081 tcgtagtcaa ccccgtccac gctgcagaac ccggcctttg cgatctgtgt ctcgctatcc
    58141 gagctgagag cgattttcgc ctgttctgcc gcgcttagca gtgcctcttc aggagccaaa
    58201 ccgggcatcg cttcctccag tttctgacga gataggccgt tgcgcttcat aaaacgtttg
    58261 agcgcttctt ctgcgaggag ggcatcgata tcatcgccgg ctacatcgtc gagggagcaa
    58321 cttgctactt cgacgaggca atcatcggtc attatgacca aggttatttc cacattcccg
    58381 gcgccccagt ccgccacgac gactctgcgg cattcggatg catcgatggg ctcgcgttcc
    58441 agatatccga cgagcgcagc gaggggctcg gacacgagga agctgggact aaaacctgca
    58501 gccttggctg ccctcatgat ctctttgcgt tgatcccgag tgagagagtt cgggattgct
    58561 atgacaaatc tattatcttg ctcgggcgag attttcgctt ctttcagcaa gaagttgaat
    58621 atcacttcag ttgctgtttc tgtggatatc gtgcgcgttg ccagagtgac ggtgtcgtct
    58681 gatgcaagac gcctcttgac ggatttgatg aatgcatggt cagggtctcc gagaagggac
    58741 gaatacgcgt ccttcgcttc ggttcccaca cgcggttgcc tgcgcttttg ataccaaaca
    58801 agcgacggat gcccgtggcc gtctgcgagc agggcttcag cacttttccc atcaaacgtt
    58861 gccaacatag aattagctgt tccaaaatct actcctatac cacgcatcag cacagttcca
    58921 cctcttaaca tgtttgctgc ccatgaaagg gtttctgggc gtatattact atagtaccat
    58981 caatatccga aataagaaac ccggtccaag aaagcaatac ataatcggct tttgtcctag
    59041 cggatggaat tgaccggaat ttgcgggacg ggtcctaaga acgccagttg agggccggta
    59101 aaagtgaaga atcgatgaag cggggcaggc actggcagtg ttctcgtgcc aggaaaacat
    59161 ggatgaagga tgttgttgtg attctcatgt ggtagactat tgtctagctg aagttgctag
    59221 tggcagtcgc gaagacgaag ctctgcgggg agggcgaaag cgcttcctag cctcgctgcg
    59281 cggcgttatc acatctgcgg gagcgactgc agacttatgg gtgcatgtca ctcaaataca
    59341 tcatcacggc taggaagtgt tagtattgtc gggcgatgct agcaagactt tggccaagtc
    59401 ggcagggatt gtggcccttg gctctgtact cagcaagata cttggctttc tgcgagagag
    59461 cgctctcgca tcggaattcg gcgccagcta tgcgacggac gcatatctga tggccatgat
    59521 cataccgacg ttgatgctgt ttggtgtggg acctgcagtc accaccacat tgatacctgt
    59581 ctttaccgac ttggagaagc gcaaggggcg cgatgcggca ttcaagtcag tcagttgcat
    59641 cataaatgcc tgcatcgtag tggcagctgc tgtgaccgtt gccggattgg tgtttgccga
    59701 gccgttggta agcttcgttg cgcctggatt ttcaggggag acttacgaac tgacagtgga
    59761 cttgaccagg ctgttgtttc caatagccat ctttacggtt atcacccact gcataaccgg
    59821 ggtgctgcac gcactgggca ggtttgcggt gccggcaatg accggcctgg tgcagaatgt
    59881 gataataatc acttcgatat tggtgttcgg accgaagtat gggataacgg cggtggctgt
    59941 tggaacggcg ctcggggctg catcgatgtt tctggtgcag ttgccagcgt tattctcatc
    60001 tgggttcaga tatcatttcg cgctcgactg gcatgatcaa gggcttcgcc aggtgggcaa
    60061 gttgataggc ccgatcgtcc tcggctctgt tgccggacaa gctggcacgg tgattatacg
    60121 gatattggca tcgaggctcc ctacagggtc tattacgtat ctcaattact ctcagcgcct
    60181 tgtgggcttg cctgtaggcg ttttcgggac tgcgctggtt acggtcttgt acccgacttt
    60241 cgcgcggatg cattctgagg ggactagaga ggactttgcg aagacattcc gccgatctgt
    60301 gggcgtagta ttctttacgc ttgtgcccat ggcggtcggg cttgcggtcc ttgcaactcc
    60361 cgtggtccgc ctggctttcg aacggcgggc gttcacgcct gaggccactg ccgccacagc
    60421 tgcagcactg gtctatgctt gcgtggggat accggctatg agcctgtctg atctgtcatc
    60481 gaaggctttt tatgccatgc aagacacaat cacgcctgtg ctggtcaata tgggggctgt
    60541 tggggtcaat gtggcactca gtttggcgct tgtagagcct atgggccata cgggattggc
    60601 tttggccggc gcatgccagc cgatagcttc ctttttgatg cttcaagctg ttttgagata
    60661 tcgcgagaag aaatggacga ggaaggctag aacagatggt gtcgctgaca attacagcct
    60721 ggccgggtca atcgccaagt ccgttgtgtc tgttgttgtg atgtggtttg ctgtatcgta
    60781 ttttgatgct tggataacag aggtgtttcc gggtatgggg acagggcggc agatagtgcg
    60841 gctggctgcg tcagtcggcg ttggagcagt tgtctacttt gcgatggccg cgatgcttaa
    60901 aagccaggaa ctctcatttg ccttcagcac cgcaaaaaag agggtcaagg agaggaaaac
    60961 agcagtgtag gcaggggtaa tcttgatgct ggctatggct tttgccagtc ggatattgcc
    61021 cggaggggcc tattaatcca gtgtattatg ttgtgttgag tgcatcagtc gtacccatgg
    61081 gctgcgcttt ttgccccatg gcgcgactcg gtctgcagat gctaggcgca gctgatttgg
    61141 ggcaattgac ggaggtcgga tatgcgagtt catctagatt gtattgcttg tactttgagg
    61201 caggctctag aggcggcccg cctcgctacc gctgatatcg atgttcagga aaggataatc
    61261 cgcagatccc ttgagacact tgcggattat gacctatata ggaccccggc cgagctgggc
    61321 agtgcagttc atgagcttgt caaggagtgt tcgggcaacc cggatcctta ccggaatgtc
    61381 aagaaagcta ccatcgaaat ggcccatgcc atttaccctg ggctgaaaag gtttgttgag
    61441 gagcaggacg acagattgct cgtggcattg gaagtttcgg cggtggggaa tctgttggat
    61501 gcgggggtat atggggatct gcgcactgtt gatctggaag agacgctcaa agaggaactg
    61561 agcaggggct ttgccgtgtg tgatattgac tttcttcgcg ccgatttgct tggcgcaacc
    61621 aaggtgctga tcattgggga caatgcaggt gaaacagtgc tcgatcggat tctgatatct
    61681 gagatcaaaa gggcttgcgg aaaagaggat aaggtgaatg tgttctatgg agtccgcagc
    61741 gcccctatga taaatgatgc aacagcggaa gatgctgtcg catcagggct ggatcatgat
    61801 gcaactattg tggatactgg ctgctgcaca cctggactcg ttcttagaaa tgccacgccg
    61861 gactttttga agctgtatga ggaagcggat gttgtgatca gtaagggtca gggaaactat
    61921 gagacgctca gcgattccgc agggcgcact attcacttcg tgctgaaggc gaagtgcagg
    61981 gcagtggccg aggataccga ggtaaacata ggcgagtatg tgctgcttcg gcggtgagca
    62041 attctatttt tgacctgttg ctttccacct gagtttgcgg gcagtttggg actgttaatc
    62101 tgagaggccg atgcttttag catcggcctc tgccacatg
//
LOCUS       DAOSYL010000006        50657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_561_length_50657_cov_7.683016, whole genome shotgun sequence.
ACCESSION   DAOSYL010000006 DAOSYL010000000
VERSION     DAOSYL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 50657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50657
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_561_length_50657_cov_7.683016"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLU15_02375"
     CDS             complement(<1..228)
                     /locus_tag="PLU15_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29797.1"
                     /translation="MYKEACRHFATEVLCAEPEPDAVEYLDMAFRLNVLTAAAGKESP
                     NVATRRRPPDETVGRWHSIISYESCAAPKTYS"
     gene            947..1528
                     /locus_tag="PLU15_02380"
     CDS             947..1528
                     /locus_tag="PLU15_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29798.1"
                     /translation="MKRPTRFIIAMVVLLVPLTLTGCGMSPDKTVAKFLDAFKAADSR
                     EMSKYVLGGNTSNLEDFDDQDPEAAEVALSVLSKLTYSIQKPAIDGKTATVPVNITSI
                     DMPRLMANLLPQMFGAALASAFSEDGGESVEELSMRLTLNAINDPNVPMTTTRVHFPL
                     TKERGKWLIMPEDELGVSLSNAVTGNFADLFGD"
     gene            2606..3280
                     /locus_tag="PLU15_02385"
     CDS             2606..3280
                     /locus_tag="PLU15_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29799.1"
                     /translation="MRSPRSSYTFLILPLALGIMLTTTGCFMGTETIDVEYLPGFQAE
                     RLFYNEQPSIAVNKIWDERPQKDRVGEGYNMYGSKVETWITRQTPTDILEAALIKQLE
                     SMGFVVVMTSGWDLAADSIPEYITSDFVAGGRLKAFWVESRPGLVTVSVNSRVTFDLV
                     IADVETGKVIWAGQFSGSDQPETIIRLDSDMRDSLSRSLTEAVNSAFQDDSVRRAFSN
                     ALQVRF"
     gene            3360..3845
                     /locus_tag="PLU15_02390"
     CDS             3360..3845
                     /locus_tag="PLU15_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29800.1"
                     /translation="MKKSCVILVALLVILCSYASSSVEASTVIGLKNGAGAGILGLEI
                     ENEFDSGLAVGIVGGLAAVLNPVTLDPIAGVSAAGTVRYYIATSGNVRPFGGGFGGIV
                     AISDGTTNITIPLFGVTGGAELRLGKFRITGELGYGGTVLESEGFIGALASGVSLGYI
                     F"
     gene            complement(4184..>4609)
                     /locus_tag="PLU15_02395"
     CDS             complement(4184..>4609)
                     /locus_tag="PLU15_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29801.1"
                     /translation="YEDYLRLPDEERYYYEVFGGCRFRTTGQSILKTKSCEAHALKDN
                     AYVLRSSGLDTGESTHPGLASDLAALWGRRVFSVSPEESLSDSSACIVRSSDFGRPIR
                     LRYFLRVKRADPVQFDLCRDTAVFGFFVSALGFLRVHWG"
     assembly_gap    4610..4709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4768..4941)
                     /locus_tag="PLU15_02400"
     CDS             complement(4768..4941)
                     /locus_tag="PLU15_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO29802.1"
                     /translation="MSQHTLVTVQELADMLQLSVATIWRYTRTEQILYVQLLNRQYCY
                     DIEKVMDHLADRV"
     gene            complement(5241..5756)
                     /locus_tag="PLU15_02405"
     CDS             complement(5241..5756)
                     /locus_tag="PLU15_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29803.1"
                     /translation="MASCLTGESRGGRVVGVALILFMMTAASGCGGNKPSSDPRSTPN
                     GTLSELEKAIRKEDITAILRCYTDPYYVTNDYETTEITHDIARIAYAIIFAAYDYSTW
                     SLADRNISMSGSEAFASCTQKMYFEGEWMSAPAMYRMCIVGSRWYIYEEIVVDSGYDF
                     SPSVRMKDLAK"
     gene            complement(5852..7246)
                     /locus_tag="PLU15_02410"
     CDS             complement(5852..7246)
                     /locus_tag="PLU15_02410"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOO29804.1"
                     /translation="MARVLGFCMRTEGCTVGRSGSGREAFGSRVGFVLSTAGAAIGLG
                     NLWRFPWLCGENGGGAFLLIYLGIMFLVGIGLFMCEVTLGRATRRCSVGAFSEIRKSW
                     GWAGVLGPFTSFLELCFYSVVGGWVICYFLHALAGFGNMTPSESAQLFADLTSDPFLP
                     IAFHGVFIGATMIICLKGVRGGIERFSMMMTPLLFVLVFVLVVHALALPGSREGIRFY
                     LVPDFSKVTAATFRDALGQVFFSLSIGTGSILTYGSYLGSKENIPMLSVAVSLMDTAI
                     AFMAGLIIFPVAFSYGFEPTAGMGLTFITLPAVLGEMPAGNLFGALFFFLFLAAALTS
                     SISYLETIVACLIEAKGWNRGLAVTLSGVLVFVVGCVSSLSLGLLSGFTVGGLSLFGL
                     LDWLAVVVLQPLGGMILAIFVGWVWGKNNVLCEVTNGGEIRFRPGSAWVDVVLKLIAP
                     ILIVVIILAGLGLI"
     gene            7636..8781
                     /locus_tag="PLU15_02415"
     CDS             7636..8781
                     /locus_tag="PLU15_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO29805.1"
                     /translation="MRLNRRLITALLCLILMSLCSIAAAQRETEIRIGVYEPMTGAMA
                     AGGQMTMEGIQLAHEQRPTVLGLPVKVILVDNRSDKVEAATAVTRLIQKDKVCAIIGS
                     YGSALSMSGGPVAQAAKVPVMGCSPTNPLVTLGNDYYFRACFIDPFQGRVMAQFAYEK
                     LGARTAAIIQDIAQDYAVGLANFFKQAFVEMTGNPKSIVAFTSYQTGDRDYTAQLTYV
                     ANAKPDVVFAPGYYADAAVLITQARSLGLKMPFLGGDTFEAPEFIQIGGKNVEGVYFS
                     THYSADAPVTPASEKFVALYKAKYKIAPNTFAALGYDCYNLVLDAIERAGSSDPKAIR
                     DAMAKTKNFMGATGIITLDKNGDATKSAVVLVVKDGQFKLVDVVHPR"
     gene            8897..9790
                     /locus_tag="PLU15_02420"
     CDS             8897..9790
                     /locus_tag="PLU15_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO29806.1"
                     /translation="MTVEILLQNIANGITLGSLYALLAIGYTMVYGILGLINFAHGEI
                     FMVSTYFAFYGTAVFALPWQVAFVLAIVLTALLGVVIERAAYRPLRDAPRISVLISAI
                     GVSFLMANLGLVIFGGRPKTFYRPPIFQTPIELGGVKVVALFFVIPLLTAILLVILNY
                     IVYRTKTGLAMRAAQWDFDTARLMGVDVDRAISITFAIGSALAAVGGIMWGLRFAEIN
                     PNMGLFPGLKCFIAAVFGGIGNISGAVLGGFILGFGEIMLVALLPGLAGYRDAFAFIV
                     LILILLVRPTGLLGDKQRVKI"
     gene            9790..10866
                     /locus_tag="PLU15_02425"
     CDS             9790..10866
                     /locus_tag="PLU15_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO29807.1"
                     /translation="MAGKSILKPILKIAAVCAALVLFFLLLWWGQEHWDAYAVRILNL
                     CGIYIAITLGLNLCNGFTGQFSLGIAGFMAVGAYTTALLTMAPEIKQAVYFIEPLIYP
                     LNSIQWGFFPALLMAAVVSGIVGFLVGAPAIRFRGDYLAITTLGFAEIIRVVFTNIVP
                     ITNGALGLKGIPPHTDLVWSWGFALICIAIMARLMKSSFGRALKAIRDDEIAAEAMGV
                     NLFSHKCLAFVLSAMMAGVGGGLLGNLITTIDPNMFRFPLTFTVLMMAVLGGLGSITG
                     TVISAVLVTVAMEALRALEEPRVIFGLALPGIPGMRMVVFSIALMIVILFFPKGFMGD
                     REISLDRAKRFATRLCRTNTSRQG"
     gene            10873..11673
                     /locus_tag="PLU15_02430"
     CDS             10873..11673
                     /locus_tag="PLU15_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29808.1"
                     /translation="MSKNTVPALVCENVTMKFGGLTAVSDFNLRVPEGALYGLIGPNG
                     AGKTTVFNIVSGVLRPSAGAIKLFGDDITGLSPDRIAYRGIARTFQNIRLFQNLTVLD
                     NVLVGLHLRREVNLAQALLALPQHRAEEARMAQTATQLLEEVGLDDFSDYRANALPYG
                     QQRRLEVARALATKPKVLLLDEPAAGMNPAETENLMRLITDIREEFGVTILLIEHEMK
                     VVMGICERMTVLDHGEIIAEGTPAEIQRNERVIKAYLGGGAVVGNKGS"
     gene            11651..12367
                     /locus_tag="PLU15_02435"
     CDS             11651..12367
                     /locus_tag="PLU15_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29809.1"
                     /translation="MLEIKDLEVYYDGIHAIKGVSLTVPQGTLVTLVGANGAGKSTLL
                     RTICGLKKPSSGRLLYEGHNLLDMGVTQIVKAGITMVPEGRQIFPDLTVIENLHLGAY
                     SRTDQDEIARDRERVFNLFPRLKERAAQKGGTLSGGEQQMLAIARGLMAKPSLLLMDE
                     PSLGLAPILVEEVFRVISEIHSEGVTVLLIEQNAQAALAIADYGYVMESGTIVLQGNG
                     ADLLNDEAVRRAYLGQDVTA"
     assembly_gap    12677..12776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12902..13264)
                     /locus_tag="PLU15_02440"
     CDS             complement(12902..13264)
                     /locus_tag="PLU15_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29810.1"
                     /translation="MGMMTASSRLNWAERQSKVAGLDESVNMGRAYEIGFEVFGKFCY
                     GVYGVHCWASGVADDRLHLDVPDALIDLRGVPLTITLQNCILRPLSPGSVDSGTQFLL
                     WRDSIARSHLDMSPEKTE"
     gene            complement(13212..16862)
                     /locus_tag="PLU15_02445"
     CDS             complement(13212..16862)
                     /locus_tag="PLU15_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO29811.1"
                     /translation="MSEWTPSQRRAVFDTDRSMVVAAGAGSGKTRVLVGRYLYLLESG
                     LADLDEIVAITFTEKAATEMKGRVWSTIVQRQAEAEACGEDAGRWRDLSRRLASQARI
                     STIDAFCSRLVRDYPLEAGVDPHFTVLDPLDASRLIDQAAMEAIDALIREGDENMARL
                     IADFGVSRVAKGIAYVYGKMRTTGRPMQEFARISSEKIRRCAEESKDGLAEEWDDIVD
                     TLAAGGADVGLLCALDWDSPEFIEEFLKIHSTAQPVRSVVTAKELDKIAKQVREKAAN
                     FVYADMMVSTVETVVRACEVLDETYLWLRGNGASMDFAGLELAARDLLRDTDAGERVR
                     KQIKFVMVDEYQDTNSLQHEIVMELAGDPPGNRLFIVGDVKQSIYGFRGAEVEVFKGA
                     MDDAQMDGGYAGMEDLGQNFRSIDPLVQLTNYVFSPALCDIWTSDATAFRSMPASAHP
                     RTELHVITYGKSELGRASLAEADLVASRIAQMVADGEQLVCEHDRETGELVGTRPVRY
                     KDIAVLLKTTSRLENFEAALERYEVPYYVVSGRGFYGRPEVVWVVNMLKAIDDPCDDL
                     SLAAVLRHPVFGLSDESLLRLALAGGLAHAFCDMDVAVDGWEANERKKLGRARECMES
                     LRRLAGRLSPGGMIQAVLDATQFEPYLAMTADGLKAIGNVHKLAEMAREASRSATSIR
                     DFIDMIERAAIDDAKEAEASLVEEEADVVTVMTVHKSKGLEFPVVIVPELNRGTGSWN
                     TPIFEFAADMGIAVPISPEIRYRDTSFGSLVADELACAENEESMRCLYVASTRASDYL
                     VLTSTLEASANLMSLDGAPEWLMPYGQAVVGSFFAAAGGDVASGCGDAEEVFEAVVAL
                     GPGSEALVHVRMLPLAGYMWDKGAEEAGKGPYVSGLALEPAQEAATVPTGSAVLRAES
                     VADQFRLVGPVKVAPDQDDPVQVSVSALMCLDRCPRWYVYEYVLGLSRVQGSLVAPSV
                     GMEMEMRLNAAQRGSVVHSVCQWARNENEAMTLLKKQLGAYGLRGRAFVESEEELWPL
                     IRNYLDSPEASAEGRREQAFLLDLGAAVVSGIADYIAVDEFMVGTVVDLKTNRLDDEG
                     IDLAARDYRTQMDAYALAVHRGLGAKRVTAVLNFLHPNVRVALEYGQEEFAEAEERLI
                     RLAARTADCRLESTKPEWSRHCRVCYYSRMCWPERDDVDAVQPLDAWHEVEPEVGDDD
                     GFLEAELGGEAE"
     gene            complement(16855..20082)
                     /locus_tag="PLU15_02450"
     CDS             complement(16855..20082)
                     /locus_tag="PLU15_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO29812.1"
                     /translation="MELRTCPCSSNYLGQAVDAVKAGRFGRFALVLRGSQEAAGARRA
                     ILTQVDAGAVFAVPVYTFDQLATVLMRRLDIEPKGSVSEIQRELLVSAALAQCKIAGE
                     LGPLAQGAERDGVARSLAELFAVMDGAGLDPERLEKALITGSGGGMEMVSNFGHDAVD
                     NLSPCINANRIPSAHAADVLSVWRRYCELLDFTGRAERGRLYSAIAQSLRSAEGRSRH
                     GLESVRVAGYAWIGGLEAVLLSALAEASVDVEIELRCPPGHGGYALDAMEEAVSPARV
                     VGVELPARQTPPRLIAAAGPRREACAVAREIKRLIVDEGVRPAQVCVASADSEGFVPM
                     FLEALDEFQVPATVSRKRLMIESSLVLDCLEVARLLGAIGRPFDIMPLVASPYLGLGP
                     RRAGQIARAINLKGLELPLAGWEAKLEKCDPSGDCDGQRYAALLAEAAEQAEDVRRAS
                     GSSGLAGYVDGFRAALARLGMPDRIFALPGDPMLGAEEWMAWATFHSLLLEISNARDV
                     WDASREPTASSMAGWTDFYSLLSFAAGRRSFALNRPSPNGVEVMGLDRAASCDREFLF
                     VSGLGEQMLPRRRRRPWMLQESDVQALARAGVSLEPMGNGAEADRHVFNTLVHSRAER
                     IYLSYATAKEDGSLARRSFFVDEHLRSLGVSDADEHEITQTVPASSVFPMRWDDVASK
                     REARMRSLWSMGRVNAAGMGSMPAEPKPEAGACLWLRNRDVAFAKLAEVWLRRQVAEI
                     AEEDGYGHFRGCIGCDELLEKMAATVFDVRPWSANSLNEYIKCPFSFFCQSVLGIEPP
                     DEVGDDVDARDKGTCLHEIVKEFISRHIGEPLDPNKADQYCEEITRIASDVVDRTPSE
                     RSGVPASVWDAYFAGIIAASRDFVIKELEFTEATGGVWRPIHLEWPFGSSVRQLAVGE
                     GRVPVTGRIDRIDAGPGGALAIYDYKSGKTVPSARNIKEVADIQLGLYALAAESLLKA
                     EVAGVWYYQVPSLGRTEGVWRAGYHQHFNAGRKRAGKLDDDQWRAFAELVEETVERCD
                     RGARQGMFSPQPSEEACRYCSFPDVCRVVELDAGDAFGKGGGQDV"
     gene            complement(20261..21205)
                     /locus_tag="PLU15_02455"
     CDS             complement(20261..21205)
                     /locus_tag="PLU15_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOO29813.1"
                     /translation="MKEKLRVRAVAMVVLVAAIVFGSTVCIGAAEVPASGRLRVGIIS
                     FTGSFNYQFVQYASNCLSDTLVGIKRFEVVEREHIERILSEQRFQVSGAVNPDTAVRL
                     GELLGIDYAFVGSLDRCECKSEFWTDKDGKRHYYYAGSAEASIRIIDMSTGAILSSFR
                     VTGTGSDNSSTSAACMNAIRSCFGSSLQTKIASAFPIVGRVLEFRSKKEVYIYTDEEG
                     VLTRGSDFRIFRPNVISVGDPGMQHQFMSADEVGVVRIEAVHGTVGRGKVVESSGEIM
                     AGDYAVEIQGYMTAGEAITGILGVVALIAMLALLSSLQ"
     gene            complement(21462..21779)
                     /locus_tag="PLU15_02460"
     CDS             complement(21462..21779)
                     /locus_tag="PLU15_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29814.1"
                     /translation="MCSTLSWAAQGVADEEKLGELMAELIELTFDFEDAIGNVNNLVE
                     AQNVSRAEWVRESIDEQSDRAWGLAALSDQYTDDGDIMEGQVFGFLGAWARSTLSGLR
                     ALD"
     gene            complement(22582..23070)
                     /locus_tag="PLU15_02465"
     CDS             complement(22582..23070)
                     /locus_tag="PLU15_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29815.1"
                     /translation="MQTENREKPMPGPRRGKAGSVLGWTVLLIVLVIAVFAVAGELIF
                     RHGAVPLPAGAKLQHISSVRLRDLDGDFEVVAIDVGKVHGVQPALEISISFWSMMADF
                     EISLVGPNDDVMWREEYNAKMARVSTPKISFETAARGQWRLILSGRARNLMLSVTWKI
                     DE"
     gene            23506..24051
                     /locus_tag="PLU15_02470"
     CDS             23506..24051
                     /locus_tag="PLU15_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOO29816.1"
                     /translation="MIEPGRALAISIDFQDKILATVEDRDEIIAQATSFVKVARLAGM
                     PVLRLEQNPDRLGTTTDLMRDALGDSPVFPKMTFSALRSPEIESAVRQSQAEQLILLG
                     VETHICVAQTALDAMAIGYEVYVLADASSSVKSFDRKTAIERMRHAGAVISTAQAAAF
                     EIVGAAGTPLFRQAKSLLKEM"
     gene            24199..24723
                     /locus_tag="PLU15_02475"
     CDS             24199..24723
                     /locus_tag="PLU15_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOO29817.1"
                     /translation="MARGAMFGAITAVLAHISFPVPFSPVPVSGQSFGAMLAGLLLSP
                     KAAAASQLVYLLAGAVGLPVYAGGHAGIGVLLGPTGGYLWGMAAGAYVSSWIVKMHIS
                     KGNGLTWQKCAIASAIGGVAVVYIFGVVQLSLATQMPLPRAFVVGALPYLPGDLLKAA
                     FAGTLGNRLATYRI"
     gene            complement(24854..>25260)
                     /locus_tag="PLU15_02480"
     CDS             complement(24854..>25260)
                     /locus_tag="PLU15_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOO29818.1"
                     /translation="ERTVSTIVGAKTSAGARRLIVGESMVCRGNWLAYPGHKHDEDVL
                     GEETRLEEVCHFRFRPERGFGMGRVYSPKRRFDQSYTIENGDTLAIPFGYHTFAVAPG
                     YDAYCLWALGGERRQLLLREDERHAWVRHIGR"
     assembly_gap    25261..25360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25361..25891)
                     /locus_tag="PLU15_02485"
     CDS             complement(<25361..25891)
                     /locus_tag="PLU15_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOO29819.1"
                     /translation="MSLKKTMPDRACSVTGVREIASPTDPQLGLAYVHLGMVSVEADE
                     DRAFNFGPLETVAVILCGVAEVAWEDEDGVERRETIGGRRDVFGGPPWAFFAHPFTTF
                     EVKGLSPFVEMALVRAEQPEPGPDCLYECGCVEGGCETMEMRAGAEETVCGRELRLDC
                     GVEIISPDEVVCRKLGE"
     gene            26027..27691
                     /locus_tag="PLU15_02490"
     CDS             26027..27691
                     /locus_tag="PLU15_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOO29820.1"
                     /translation="MKRKRITTIIVSTLLIFVASSVWANSEDDAWRKKITEFYAYDTA
                     QPFEISFTLLNDNQWYLHYDVSYKSEGDTVYAHYFVPVTAKSQPCPCTIGLHGVFSDS
                     EEQFWMMADFLAKRGIAALAPSLPQHHRRAKGKQFVSGQRFVVGTPETIRDNLRQAVV
                     DQRRAVDWLSMRPEINPSAISIAGVSMGGIVGSLTYKIEPRIRNGVFVVAGSGVDGIV
                     DNSDLSVVSAFRQAASVKLLNPQKFIDTLQIVDPINIPDLYPRPVLMMNGTSDAIITP
                     AEALRLYNTLGKAEQIWTNGSHYYPAYAGEYLTASFLASHMPKSYGDTPPGSVLVASP
                     GYRFIEKVQPPVAGSNRLWVDLKVQIAENSKELQHKVAVPLVNRMVPVIVASRETYQR
                     IERDIINLPAFIYIMESDDASQFAAALAYIAALDLTANPLVYYAAPSQGTSCTLTQFT
                     AVQVESARHILASILSVKSSGATPVSVPKGLGSLIAQYPNHAQAGIALGKMIRYENLG
                     PLPWLPTWQDQGVRWSLGPEEPDQPKNIRREDKAGIAPWTNRSNNV"
     gene            complement(27896..28423)
                     /locus_tag="PLU15_02495"
     CDS             complement(27896..28423)
                     /locus_tag="PLU15_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOO29821.1"
                     /translation="MKNKLTQKEQSLLSDQLNHEKLCIQKYNNYAQQAEDPQLKQLFS
                     QYASQEQEHYDTINQLLQGNQMIISAGEGGTEMIQGGPGVGRYSMEVGTIGQHASQTA
                     NHGMTGLRNQNDATLVMDMLATEKFVSSTYDTGVFESANSNVRQALQHIQQEEQQHGE
                     GLFNYLSEHGVYNPQ"
     gene            complement(28547..28705)
                     /locus_tag="PLU15_02500"
     CDS             complement(28547..28705)
                     /locus_tag="PLU15_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29822.1"
                     /translation="MILMIGDVKIEITRKESNNLEETVRRDSVAKGAWEARRAYESAA
                     ISYIAGFR"
     gene            29033..30331
                     /locus_tag="PLU15_02505"
     CDS             29033..30331
                     /locus_tag="PLU15_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOO29823.1"
                     /translation="MAIDTPNWIKSSVIYEAFPRNHSKSGTFAGLCHDLERISRLHAD
                     VLWLMPIHPIGAANRKGSLGSPYAIRDYRAINPDLGDEQSFHELINKAHALGMKVIID
                     VVFNHTSRDSVLLAQHPEWFLQDDHGNPATKVPDWWDVYDLDYSQQGLWDYQIETLRK
                     WVQMGVDGFRCDVASLVPLDFWLTARKELSRGRKENLIWLAESVDKSFIKILRDTGWT
                     AHSDPELHAAFDLTYDYDGFDYLKGYIQGQCRLDAYLNHLYIQETLYPAHAVKMRFIE
                     NHDNPRAAEMIRDKGSLMNWTVFYTLLPGATLVYAGQEIASAHYPDLFGADPIDWANG
                     DYEFTAFMENALELSKKIKGTCKHFSVSQLVDGVVKIVWRGSGSSYVALINLAGKYGE
                     VCLDELKECHAEVCLGRREKAQEHGRCRLANTPLVVRTPA"
     gene            30610..31188
                     /locus_tag="PLU15_02510"
     CDS             30610..31188
                     /locus_tag="PLU15_02510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO29824.1"
                     /translation="MLRGNRVVLREFRKEDAGRIQEWIGNENITKYLGFMMFPQTLEE
                     TEAYIEQQLRKEHVPSEGQFVIALADDPQLEYVGAVGLHQIDWRNRNCELGIVIGREE
                     MLGQGLGGEAIDLALGFAFDFLNMHKVNLTVFEYNERAHRCYLKCGFRDEGRVRQQRY
                     YDGHYWDMILMGITKDEYVQARRENTTNVCYI"
     gene            complement(31680..32186)
                     /locus_tag="PLU15_02515"
     CDS             complement(31680..32186)
                     /locus_tag="PLU15_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29825.1"
                     /translation="MIGLVSIIIFWAAIVPLITLIHNLGHAAAALALVPEHGVVVRLG
                     SDPKISLVRRGRLEVLLQPWSGWVGRWGWTAGVVEARRAHFVWIMLAGPLASILAALL
                     FGVVKSMFAAGPLFMRDLAQAGMVAAIIQFAASILPVTYPSWLIGYSDMWSDGLRLWR
                     CLAGKTTE"
     gene            complement(32297..33355)
                     /gene="ltaE"
                     /locus_tag="PLU15_02520"
     CDS             complement(32297..33355)
                     /gene="ltaE"
                     /locus_tag="PLU15_02520"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOO29826.1"
                     /translation="MEWIELRSDTLTLPTEEMRDAMRNAKVGDDVWGEDPTVIRLEEL
                     AAKKVGKEAALFLPSGTMGNLVAVMTHCSRGDEIILEAESHIYYYEVGGLSAVAGAVP
                     RLVLGNSGVLDPDDVRAAIRESNIHYAPTTCLAIENTHNRGGGTVTDLTILAALHDVA
                     KDRGLSIHMDGARIFNAAIALGVDASQIACHVDSVMFCLSKGLSAPVGSMLAGDADFI
                     ARARKNRKMVGGGMRQAGVLAAAGIVALEKMVDRLAEDHANARALAEALVDVPGIEID
                     LSTVETNMVVFDLSRLGIGSNEFAAELSAEGVRVSTRPPHGLRAVTNRHFTADDVERA
                     AQAVRTVCCRHAAPCSGA"
     gene            33664..35214
                     /locus_tag="PLU15_02525"
     CDS             33664..35214
                     /locus_tag="PLU15_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOO29827.1"
                     /translation="MGDNDRLRELKAQREKVIQGGGPKRIEKQHASGKLTARERIERL
                     LDPGSFTELDMFVEHRCQEFGMPEQELPGEGVVTGYGTIDGRKVFIFAQDFTVMGGSL
                     GEMHAKKICKVMDLAMKVGAPVIGINDSGGARIQEGVDALSGYGQIFYRNTIASGVVP
                     QISVIMGPCAGGAVYSPALTDFIFMVQGANMFITGPQVIKTVTGEEVSADELGGPTTH
                     NTVSGVAHFMYDTEDDCFLAIRKLVSYLPSNNLEDPPVQALENASSAEIIPELREIVP
                     EESNRPYSMHDVILRFVDDGDFFEVHQHYARNIIVGFARLGGRPIGIIANQPQVAAGC
                     LDINASDKAARFIRFCDAFNIPIINLVDVPGFLPGVNQETGGIIRHGAKLLYAYSEAT
                     VPKITLIVRKAYGGAYLAMCSRDLGADVVYAWPDAEIAVMGPDGAANIIFRKEITAAQ
                     DPEAARTEKIAEYRSKFANPYAAAKRGYVDDVIDPAETRRRLMDALDMLSTKRETRPP
                     KKHGNIPV"
     gene            35230..35646
                     /locus_tag="PLU15_02530"
     CDS             35230..35646
                     /locus_tag="PLU15_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOO29828.1"
                     /translation="MTVGEELILGLQTLVLGMVVTFIGLAILQVIMILMGRASDRKRA
                     PAGYTVGDRAETEIRDEITASDDEELTHRGSEFEGRAEDYSEEQVAAIAAVMAILMEE
                     GAAPVHVKSVTQVGPVAANLWGLAGRQDLMSGRSRA"
     gene            35696..36094
                     /locus_tag="PLU15_02535"
     CDS             35696..36094
                     /locus_tag="PLU15_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOO29829.1"
                     /translation="MRRFRVQVNGTTYEVEVEEIGGMGTPAAAQPAPAAPTPSAPVSA
                     GPAKPAASKKPAASGRGTSVTAPMPGTIIDIKVKVGDKVEPRTVVAMLEAMKMENEIF
                     AGRSGVVTHIAVEKGASVNTGDLLVSIDPA"
     gene            36111..37241
                     /locus_tag="PLU15_02540"
     CDS             36111..37241
                     /locus_tag="PLU15_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOO29830.1"
                     /translation="MQEAVVEFLYSTGFAVLTWKELVMFAIAGCLMYLAIVKKFEPLL
                     LLPISFGMLLANLPAAGLMEGPQDGIVGGLLYYLYQGVKLGIYPPLIFLGVGAMTDFG
                     PLLANPSTIFLGAAAQFGIFITFLGALLLGFSPAQAGSIGIIGGADGPTAIFVTSRLA
                     PELLGPIAIAAYSYMALVPIIQPPIMRALTRKEDRQIVMEQLRPVSKKEKILFPIIVT
                     VVVGLMLPSAAALVGMLMLGNLFRESGVVDRLSKTAQNELINIVTIFLGLTVGATASA
                     ESFLTWQTISIILLGLVAFSGGTAGGVLLGNLMCRLTKGKVNPLIGAAGVSAVPMAAR
                     VVQTVAQDENPGNFLLMHAMGPNVAGVIGSAIVAGVLLSRFG"
     assembly_gap    37392..37491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37492..37871)
                     /locus_tag="PLU15_02545"
     CDS             complement(<37492..37871)
                     /locus_tag="PLU15_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29831.1"
                     /translation="MLHDFYTFVENIAKTVAMRIDGHDWHRELLEQMNPRTLSAAFVQ
                     RHSCASRQVPYVFRNIYGFQPDADRTLELVCDMHKTVSALNSDLDCHVREIDRITRWK
                     PEEPLATSGERREHPPSVWPCIGR"
     gene            complement(37929..38357)
                     /locus_tag="PLU15_02550"
     CDS             complement(37929..38357)
                     /locus_tag="PLU15_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29832.1"
                     /translation="MGVSGVLPQFAPQLVDELQLLLGGVTMVDDGIHGWNVPEIREAV
                     FWWSEERAELVMADAMERARRIADLLGTRYSAPAVTLYGSLAEGCILVEGFRGDYWEM
                     YVMADEIADPLSMRGKEGSAFQTRGLPGDSGPNSQGTRES"
     assembly_gap    38565..38664
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(38805..39512)
                     /locus_tag="PLU15_02555"
     CDS             complement(38805..39512)
                     /locus_tag="PLU15_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29833.1"
                     /translation="MQAKRLRKLNVSMIVAVTLVVVTAGACSAQVGGGGNSVGWVFLR
                     VDDLNESLVDAEFNQLPGGMLTWGGYGTVGVGNGKWSIGGWYGGGEAISKPTAGAEPK
                     IATLAFNYGGILVEYSAVDSPKAHVGLGTVLGGGMASLTTKRGMISDFDEALGAASSA
                     SVWRPYLLAQPQASIAFPLSALIDITITGGYSLLYSPTGWMDGMTFKDSFDGPMKSAG
                     LPFVQLSFSFGPPPLGI"
     gene            complement(39528..39929)
                     /gene="mce"
                     /locus_tag="PLU15_02560"
     CDS             complement(39528..39929)
                     /gene="mce"
                     /locus_tag="PLU15_02560"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOO29834.1"
                     /translation="MVKSVDHIGIAVADLEEALKLYTETFGLELQGTEVVEEQKVKVA
                     FLPIGDTEIELLESTDPEGPIAKFIEKRGQGIQHIALRVDDIDAALDKMRAEGIRLID
                     EKPRYGAGGARIAFLHPKATGGVLIELCERK"
     gene            complement(39950..41014)
                     /gene="meaB"
                     /locus_tag="PLU15_02565"
     CDS             complement(39950..41014)
                     /gene="meaB"
                     /locus_tag="PLU15_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOO29835.1"
                     /translation="MGRTEVVSRALSGDIRAVSRLISLIEDCVPGFEGLYSEIYPFTG
                     KAHVIGITGPPGSGKSTLVDKMAAVCRERGKTVGIVAVDPTSPFTGGALLGDRLRMQR
                     HALDDGVFIRSMGTRGQLGGISRATSGAVDVLDAAGYDYVIIETVGVGQSEVEIASIA
                     DTVVLVQVPGLGDDIQVIKAGVMEIGDLFAVNKKDRPGVERVVAEIRIALELSSVASR
                     GGGGTSRIAGSAGHHGVSALGAAGSEREETVDEEAARPCWHPPILQTLAETGEGVGEL
                     FDACDEHMRYLAGTGELDDRRYRRARSETMQIASGRIWRGLTDDRGAFAAAEELAWKV
                     ARREIDPYSAGNELYEMIVK"
     gene            complement(41017..41442)
                     /locus_tag="PLU15_02570"
     CDS             complement(41017..41442)
                     /locus_tag="PLU15_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOO29836.1"
                     /translation="MGDSMGTDTGRKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVI
                     YTGLRQTPEQIVAAAIQEDVDVIAMSILSGAHNVLFPKVMDLLREKGVDDILVVGGGI
                     IPDEDIPALKECGIADIFTPGTSTSETVKFIEENVKRRS"
     gene            complement(41455..43095)
                     /locus_tag="PLU15_02575"
     CDS             complement(41455..43095)
                     /locus_tag="PLU15_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOO29837.1"
                     /translation="MDRWKSTTYGKTCARFPERREKFEGTSGIEVQPVYTPLDTEGID
                     YLEDLGFPGEYPYTRGVQPTMYRGRLWTMRQYAGFATAEESNERYRYLLEQGQTGLSV
                     AFDLPTQIGYDSDHELALGEVGKVGVAIDSLADMEILFNEIPLDKVSTSMTINAPAAV
                     LLAMYIAVAEKQGVAPEKLNGTIQNDILKEYAARGTYIFPPKPSMRLITNTFEYCSAN
                     VPNWNTISISGYHIREAGATAVQELAFTLADGIAYVQAAIDAGLDVDEFAPRLSFFFA
                     AHNDLFEEVAKFRAARRLWARIMKERFGAKKEKSMRLRFHTQTGGSTLTAQQPDNNIV
                     RVALQALAAVLGGTQSLHTNSRDEALALPSQESVMIALRTQQIIAYESGVADTIDPLA
                     GSYYVEALTTQIEEAAADYIRRIDEMGGAPMAIERGFIQSEIEESAYRYQMEIESGDR
                     IVVGVNEFYIEEEPPKGLLKVDPAVADLQCKKLADVKASRNGEAVEKSLEALKAAAEG
                     TDNLVPYILDAVREYATLGEICDTLREVFGEYKPSGAY"
     assembly_gap    43693..43792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<43793..44029)
                     /locus_tag="PLU15_02580"
     CDS             complement(<43793..44029)
                     /locus_tag="PLU15_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29838.1"
                     /translation="MFPILRSMWMLRDKQVWIPMPGTNQKMSVVGAIDIRTGAYACFT
                     VVSPLLLFFRYHEMLWPLKLESNRWISLVSIKLHA"
     gene            44435..46543
                     /locus_tag="PLU15_02585"
     CDS             44435..46543
                     /locus_tag="PLU15_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29839.1"
                     /translation="MKHQLRTHLATALATLCLLAVSAGALGSSVTLLDGTTYDADLVG
                     FFTFDGVDHFVFRLTDGYLMACPRSDVMLIQVSKSSMGSTQVGQQVPPPAIAIPSTPI
                     SGPLFELSGIWSADLNGFGGEIRIEQSGTTLSGSVFGDTLANGRVSPDGSVAFERIVG
                     SGNQVYRGTVSRDASGNLIIEGTFDCNITGGKGLPWHAVLTKPSAGSPSAQLPTTQPA
                     VPPALEPSYLQAEREPNYNIASANPIAVNTNVTGAISTDGDIDMYKVTVTTHGTFHVT
                     CTQPADFFMAILNEAGSQIAGDDLGSSGTTMVLNADVFEPGNYYILVRGYSGNIKSDT
                     PYTLRAEFREARDAFEPNDNVNLASAVVSGRQFDSFIYRPGDIDYYSVSVSGPATIKV
                     TSTQPADYYLSIVDSSGTKELYGGDLGSKGTNMLLEGDVYDPGTYYVVMRGYNKDIYE
                     MHTPYSMTITVTHGGTDRFEPNDTPGQAAVPPLGQTFAAFISRPGDLDYYKVDIAGPS
                     QVVAESDQPVDLHLSLIDRDGKTVLLGDDYGHPGNKMVLTHDIFQPGTYYVLMRGYNT
                     NIYSMHTPYNMKITVKPALYDPAEPNNSANEAKSIQLGQPVTGVIATGTDEDWWQFTA
                     PLLGKVRITCTQTADFRLRVVDATGKEITNQDLGNKGTLMDLTLQLTPLTRYYVVVQP
                     YSSSCYNMFEAYQLIVGSAI"
     gene            complement(46680..49499)
                     /locus_tag="PLU15_02590"
     CDS             complement(46680..49499)
                     /locus_tag="PLU15_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO29840.1"
                     /translation="MKANGGRTDRMDIILHGKVESVGSDTGLGPERRGKKKGQPGRGD
                     VGGTPDKKVRGLRQVDFAITGMTCAACAARVERALANEAGVAHAAVNLAAETARVQYD
                     PDQTSPDRLFKAIEAAGYGAREAARDAEEDARRTEERKQQEINNWRLFYIGAAFSIPL
                     VVGMIADIFGIEALMFLIDPFVGFVLATPVVFVPGSRFYISAARTVAHGGANMDVLVA
                     LGTGAAYILSVVHTFIVSGPVYYEAAAVVITLVLLGKNLEHVAKGKTSEAIRKLAALS
                     PKTASVLQADGTEIQVPVDEVEVGDILIVRPGERVPVDGVIIEGFSAIDESMLTGESI
                     PVDRQAGDAITGGTVNGHGMLKMRATRVGSETALAQIIRMVEEAQGSKAPIQRLADVV
                     SYYFVPAVLVVAVVTFAGWMAAGIGASQALVNATAVLVIACPCALGLATPTAIMVATG
                     RGAELGILIRGGEHLERLSSVKAVVLDKTGTITAGVPSLEEVEVFAPHGREEALRLAA
                     SAERASEHPLGRAIVEAAEQAGIDIPMPDEFRTVPGRGIVATVEGRQVAVGTLRFLDE
                     LGVSVDREGLAAERRFGSGLVAGTAEETNGAGSEVPVTSLIAAGAVGASVARMQDEGR
                     TAVVQAVDGAVAAVYGIADQVRPGSVEAIKGIQDLGIDVYMITGDNARTAAAIAERVG
                     IPKPNVLAEVLPEDKAREVERLQEAGLAVAMVGDGINDAPALATADVGVAMSTGTDVA
                     MEAADITIVGNLGKLLDAIKLAKKTITIIKENLFWAFVYNTIGIPLAAFGVLPPVYAG
                     AAMSLSSVSVVSNSLRLRGFGKERPRGISRTEVKGVDNMPYGRKSMSDSKENMVAGPG
                     STEKSAAAAGMVGDYSEALLNVAGMSCGHCKAAVEGALMGVVGVESAEVDLEAKSARV
                     VYDRNAAKPADLVRAVEDAGYEVLR"
     gene            complement(49879..50169)
                     /locus_tag="PLU15_02595"
     CDS             complement(49879..50169)
                     /locus_tag="PLU15_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29841.1"
                     /translation="MPGHGIGLGIGAPGHGIVGHPGVGMPGYGISGEGIIGSGAGAGH
                     GSATGGHGIQGPGSGTTGAGMRTPGQDMFSGGRINESTESKKKACRNSSLLI"
BASE COUNT        11758 a        15427 c        12238 g        10634 t
ORIGIN      
        1 tgagtacgtt ttcggtgctg cacacgactc atatgagatt attgagtgcc atctccccac
       61 agtttcgtcc ggtgggcgcc gcctagtagc cacattaggg gactccttgc cggcggcagc
      121 cgttagcacg ttcaaccgaa atgccatatc aagatactcg acggcatcag gttctggttc
      181 tgcacagagg acttctgtcg cgaaatgtct acaagcttcc ttgtacacca acctccaact
      241 tacaaaccat atgaagcggc caccactaga ttacacgcat gcgcaagaga tgctcgcatc
      301 aacaactgcc gtcccgaaga cgggcttgcc cagcttacaa aatcccttct tctctgacac
      361 gcgcacacat aagcctgatg tgttcgattt ccccgtcagc acagccaacg ctgcgcaagg
      421 ttctgccaca gcggacaagg gccgccagtt tcttgcttca tcgcgggttt cagatgataa
      481 cgaaggccaa aaagaatgat tagcgtagat agatatgtga gaccaaaaaa ggagtaataa
      541 accatgttct cccgcaaagc catggtgtca ggcctgcttg ttctggtctg cacctaagcg
      601 gcctcaacag aagatacccc atggctgcag acggccgaag ttcgaaacga agggagcgaa
      661 atacatgatc cggtcgagac aacggatcct tctatacgca aacacctgag ccatcagcct
      721 ggcagcggcc tcgaaacact cgaccgcagc atcgcccata tccttgaagg cgccaaccgc
      781 tccaagtatg ggtggaccat aacaatcgag gctaaacgct aattcgctac tagctcaaac
      841 accctgcaac ccaatggctg ggaattcaga acataattga agctgtcagc agcttctgac
      901 catgagacga tcagtaccta tacaaaacat gcaaggagtg tgactgatga aaagaccaac
      961 caggttcatt atcgccatgg ttgtcttgct ggtacccctg accctcactg gatgcggcat
     1021 gtctcctgat aagacagtgg ccaagttcct cgatgcattc aaagcggcag actcaaggga
     1081 aatgagcaaa tacgtgttgg gaggaaacac ctcaaaccta gaagacttcg atgaccaaga
     1141 ccccgaagca gctgaagtgg ccttatccgt cttgagcaag cttacttatt cgatccaaaa
     1201 gcccgcgata gacggaaaga ctgctaccgt gcctgtgaac ataacctcaa tcgacatgcc
     1261 caggctcatg gccaacctct tgccacagat gtttggtgca gcactagcat ccgcctttag
     1321 cgaagacggc ggtgaatcag tggaggaatt gagcatgcgg ctcaccttga acgccatcaa
     1381 tgaccctaat gttccgatga ccaccacacg tgtgcatttt ccacttacca aagaacgcgg
     1441 caaatggctg attatgcccg aggacgaact aggcgtttcc ctctcaaacg cagttacagg
     1501 gaacttcgca gacttgttcg gggactagaa ggccccgaat gatgtcatct tcaggatcga
     1561 ctgtcaagta gtgcggaacc tgcatcctct aagtaactcc caataagttt ttgcgttctc
     1621 ggttagcctg ccatatgatg tctgctaggc ataaacacgt ctttgcacct atgtcgcagt
     1681 gcggtaattg gttcgcattt gtgcatcgac aacgtataca tcttggcctt agccataaac
     1741 gcccgcagca ttcagccgcg ctgggatatg atccccaggg caaagccagg gtagatggct
     1801 cctggttcgc cgctcgcggg caattatgtt ctttagcgac gcgcatacac tcccgaccgt
     1861 gagatccgtg tgagcgctcc gacagtctat gcattcaaca cactcatatg aaagggcaac
     1921 ttggggctgc cttcaaggaa tgtgtctatt atttagtcgt ttttcgggcg cgggtgtcca
     1981 agttttagac tggtctgtaa aacctgctcg cccccgcggc caaccttggc cggctccacg
     2041 ccgcacggcg cttccctagg cttctaccga aggccaaact tacaagtaaa ggccttagca
     2101 gtcccctggc caattctcaa acagcggcaa tacaaaaact caaaggacct tcacaaccag
     2161 ataaggcgtg ccgtctatgc atttctcctt gatgatccca ctcctttcac cgggtatgca
     2221 gacaagattg ggctggataa cgttcgtatc cgatggtgtc atcttgaagc aagggcaaga
     2281 acttgactac gcaaggcaat agcatgcttt ctcgaaatct caggaaggta tactggtgtt
     2341 ttgggataat agtttgtttt atgcgccaca cattgcatcc tatctgccaa tctcaaatgc
     2401 caaaatacga tcattgcatc gtagtcacaa ttgcacttga aaaagggggt ggttgtttgc
     2461 ccgccatgta ctggattatc gcaggcgtag cgctcatcct aagcctactc agcctgaccg
     2521 gtatagtagg ttacgcctgg ccaattgtca tagctgctat cttcgtggtt gtctgcctag
     2581 cactgaaatc aaggaggaaa tcggaatgag atctccacgt agcagttaca cttttctaat
     2641 acttccgctg gctcttggca tcatgctgac taccaccgga tgctttatgg gcactgaaac
     2701 aattgatgtc gaataccttc cgggcttcca ggcggaaagg ctgttctaca atgagcagcc
     2761 ttcaatagca gtgaacaaga tctgggacga acgccctcaa aaagatcgag tgggagaagg
     2821 ctacaacatg tatggcagca aggtggaaac ctggatcacg cgccaaactc ccactgatat
     2881 cctcgaggca gcacttatta agcagttgga gtcgatggga tttgtggtgg tcatgacatc
     2941 cggatgggat ctagccgccg actcaatccc ggagtatatc acttctgact tcgttgccgg
     3001 aggcagactg aaagcattct gggtggaatc aagacctggg ctcgtcactg tatcggtcaa
     3061 ttcgcgggtt acatttgatc tggtaatcgc tgatgtcgag acaggcaagg ttatctgggc
     3121 aggccagttt agcggttcgg accagccaga aaccatcatc aggctagaca gcgacatgag
     3181 ggactcattg agccgatcac taaccgaggc tgtaaacagt gcatttcagg acgacagcgt
     3241 tcgtagagcg ttctccaatg ctctacaggt gagattctaa gggcccagcc aagtcttagc
     3301 agaaaaccgg ctgtctaggg ctaggttgag cgactaattg agtaagagag gaaagtagca
     3361 tgaaaaaatc gtgtgtgatc ctcgttgccc tattggttat tctctgcagt tatgcaagct
     3421 cctcggttga agcgagcacg gtaatcggac tcaagaacgg agccggggcc ggaatactcg
     3481 gtctcgaaat agagaacgag ttcgacagcg gattggccgt cgggattgta ggaggcctgg
     3541 cagcagtttt gaacccggta acacttgacc caatagctgg tgtatcggcc gccggtaccg
     3601 tcaggtacta catagcaacg tctggcaatg ttaggccctt cggcggcgga ttcggaggca
     3661 tagtcgcaat ttcagacggc acaacaaaca tcacaatccc cttattcggt gtcactggcg
     3721 gagcagaact gcggctcggc aaattcagaa ttacgggtga actcggctac ggtggtacgg
     3781 tgctggaatc agaaggcttc attggcgctc tagcatctgg agtatcgcta ggctacattt
     3841 tctagtgctg cactaagaaa tgcgtcatgg aacgtgagat ccatcgtgca aaagctgcat
     3901 gcagtcagtg cctgatcact actgatgagg caaccatgga gttcgcttgc aaccccgtat
     3961 gcggccggaa tcaccatccg gccgcgtatc gaggctaccg gagacgactg aacctgtttg
     4021 cagtccacga gaaatcagat ccacatgggc actccatatc acgctcacga aaagtacacg
     4081 cgtattcgcc catctccttt gaattggctc attactttcg ctttgcatag agtgattgga
     4141 cggaatgctc aaacgagcat gggctgatgg ctagagaaaa cgcttacccc caatggacac
     4201 gcaagaatcc taaagcagaa acaaagaagc cgaacaccgc tgtatctcga cagagatcga
     4261 attggaccgg atctgcccgt ttgacccgta agaagtatct tagcctgatt gggcggccaa
     4321 agtctgaact ccggacaatg caggcgctgg aatcgctcag agactcttcg gggcttacac
     4381 taaacactct ccgcccccac agagccgcaa gatcagatgc aagcccaggg tgggtggact
     4441 ctcccgtgtc aagacctgaa ctgcgcagca catacgcatt atctttcaat gcatgtgctt
     4501 cacatgattt cgtcttcagg atcgactgtc cagtagtacg gaacctgcat cctccgaaga
     4561 cctcatagta atagcgctct tcatcaggca accggaggta gtcttcatan nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaagacctca tagtaatagc gctcttcatc
     4741 aggcaaccgg aggtagtctt cataagtcta gactctgtct gcaaggtgat ccataacctt
     4801 ctcaatatcg taacagtact gcctgttgag cagctgcaca tacagaattt gctcggtgcg
     4861 ggtgtaccgc catattgtcg ccaccgaaag ctgcagcata tctgccagct cctgaacggt
     4921 taccaatgta tgttggctca tggcaatacc tcctcaatct gagcatcagc cacaagacaa
     4981 tagatgtcca gtgactagtt ggccaagggc ttgacgacta tcgaaaacca cgccatagat
     5041 acaagcggcg cagcagagat gatggattca tccaagcccc ggcctccggg cagaacttgg
     5101 ccctttgcca tgtgacagcg cctacctgcc agaggttttc gctgcattat ctctccggct
     5161 cggcaaatat ctcgagatgc aggaaacccc gttggacacg gtaccgtcgg tgtcgctcgg
     5221 gccctgtacc gatcaacttc ctactttgcc aagtccttca tcctcacact tggactaaag
     5281 tcatatccgg aatccaccac tatttcttcg tagatgtacc accttgatcc cacaatgcac
     5341 atgcggtaca tcgcaggagc agacatccat tcaccttcaa agtacatctt ctgtgtacac
     5401 gaggcgaacg cttcagagcc gctcattgag atattcctgt ccgccaacga ccatgtcgaa
     5461 tagtcgtatg ctgcaaatat gattgcatag gcgatgcgcg caatatcatg ggttatctcc
     5521 gttgtttcat agtcattggt gacataatac gggtcagtat aacatctaag tatggcagtg
     5581 atgtcctcct ttcggatagc cttctccagt tcacttaagg tcccatttgg tgtgctcctt
     5641 ggatctgacg acggcttgtt tccgccgcaa ccgctggcag ccgtcatcat gaacagaatc
     5701 agcgccactc caacaaccct gcccccccga ctttctccag tcaagcatga agccattaac
     5761 ctccatccat gtttttggcg aatttcctcc tactctaata catgcactga aatggaaccg
     5821 ttgccctttt tctctttaaa ggccgacaat atcagataag gcccaacccc gccaggataa
     5881 tcacgactat gagaatcgga gcaatcaact tgaggactac gtcaacccag gcacttcccg
     5941 gcctgaacct tatctctccc ccgtttgtca cctcgcaaag cacgttgttc ttcccccaca
     6001 cccagcccac aaagatcgcc aggatcatcc cgcccaaggg ctgaagcacg acgactgcca
     6061 accagtcaag caacccgaac aagctgagcc caccaacggt aaacccgctg agcaagccca
     6121 gcgaaagcga agagacgcat cccacaacaa agaccagaac tcccgacaaa gtcacagcca
     6181 gccccctgtt ccatcccttc gcctctataa ggcacgccac tatcgtctcg agatacgaga
     6241 tggacgacgt gagggcagcc gccaaaaaca agaagaaaaa aagggccccg aaaagattcc
     6301 ccgccggcat ttccccaagt accgcaggca atgtgatgaa ggtcaggccc atgcctgcgg
     6361 tcggctcgaa accgtacgag aacgctacgg ggaatatgat gaggccagcc atgaaagcaa
     6421 tagcagtatc catcagcgaa acggctaccg acagcatcgg tatgttctcc ttgctcccca
     6481 aatagctgcc ataggtaagg atagaccccg tgccaatact gagcgagaag aagacttggc
     6541 ccaatgcatc cctgaaagtc gctgcagtta ccttagagaa gtcaggaacc agatagaacc
     6601 tgatcccctc ccggcttccc ggaagcgcca gagcgtgaac taccagcacg aacacgagaa
     6661 cgaagagaag cggcgtcatc atcatcgaga atctctcaat accgcccctc acccctttaa
     6721 ggcagatgat catcgtagca ccgatgaaaa ccccatggaa agcaattggc aagaacggat
     6781 ctgaagtaag gtccgcaaaa agctgagcgg attcactcgg cgtcatgttc ccaaagccag
     6841 caagcgcatg gagaaaatag cagatgaccc aacccccaac cacgctgtaa aagcatagct
     6901 caagaaagga tgtaaaagga cccagcaccc ccgcccatcc ccatgacttg cgaatctcac
     6961 tgaaagcccc cacgctacag cggcgtgtgg cccgtccaag agtcacctcg cacatgaaaa
     7021 gcccaattcc caccaggaac atgatcccaa ggtagattaa aaggaacgcc cctccgccat
     7081 tctccccgca cagccatgga aatcgccaca ggtttcccag cccgattgct gcccctgccg
     7141 tggaaagcac aaacccaacc ctgctgccga aggcctcccg ccccgagccc gatcgaccga
     7201 cagtgcatcc ctctgtcctc atgcaaaacc ccagcacccg cgccaccccc gcatcccgct
     7261 cccactacgc cacccgtttc ggcagtcccc agatcacatg gtgcatttcc cctccgtccc
     7321 tacattccat gcaactcccc tgagcatgta tgccagttac cttacaggtc ggccccgaaa
     7381 atcgatgcag ggccgaaaca tgggggcaga acatgccgcc ttgcccccgc aagctgccca
     7441 atctgaccaa cccctctcgc ttcatctcgt ttctccccgt aatttccatt atgtatggtt
     7501 tctatatcct cccctttccc catacactgt actgcatata aacatatgtg ccacatgtcc
     7561 ttgaagctat cccgttgagg ttgagatcag gacacgcctc aaggacagcc tgatctgaat
     7621 aaggaggttt cccatatgag actgaatcga cgactgataa cggcgctcct ctgcctgatc
     7681 ctcatgagcc tctgcagcat cgctgccgcg cagcgggaaa cggagatcag aatcggcgtc
     7741 tacgaaccca tgaccggggc catggcagct ggcggccaga tgacaatgga aggcatccag
     7801 ctcgcccacg aacagaggcc tacggtctta ggactcccgg tcaaagtgat cctagtcgac
     7861 aaccgcagcg acaaagttga agccgccaca gcggtcaccc ggctcatcca gaaagacaag
     7921 gtttgcgcaa ttatcggcag ctacggaagc gccctctcca tgtcgggcgg gcccgtggcc
     7981 caggcggcaa aggtcccggt catgggatgc tcgcctacaa accccttggt tacacttggc
     8041 aatgactact acttccgcgc ctgcttcatc gacccattcc agggccgcgt catggcgcag
     8101 ttcgcctacg aaaagcttgg cgcccgtacc gcagccatca tccaggacat cgcccaagac
     8161 tacgcagttg gccttgccaa cttctttaag caagcctttg tcgagatgac tgggaacccc
     8221 aaatcgatag tcgccttcac ctcatatcaa acaggcgacc gcgactacac tgcgcagctg
     8281 acctacgtgg ccaacgcaaa acccgatgta gtcttcgccc ctggctacta cgccgacgca
     8341 gcagtcctca taacccaggc ccgctcgcta gggcttaaga tgcccttcct cggcggagat
     8401 acttttgagg cccccgaatt catccagata ggcggaaaga acgtcgaagg cgtctacttc
     8461 agcacccatt acagcgcgga cgcccccgta accccggcct cagagaaatt cgtagccctg
     8521 tacaaggcaa aatacaagat cgccccgaac accttcgccg cattgggata cgactgctac
     8581 aacctggtcc tcgacgccat cgagcgcgcg ggctcatcag accccaaagc catccgcgat
     8641 gccatggcca aaaccaagaa tttcatgggc gcaacaggca tcatcaccct cgataagaac
     8701 ggcgatgcca cgaagagcgc agtagttctg gtggtcaagg atggccagtt caagctggtc
     8761 gatgtcgtgc acccgcgtta atcagaataa cctttgctga ggaaaggagc ccggatagga
     8821 caggatttcg cccaggtagg ggccgacctg gcttcgcaca acacgcggcc caacgcctga
     8881 tgggaggttg aagacgatga ctgtggagat cttactccag aatattgcga acggaataac
     8941 cctcggcagt ctctacgcat tgctagccat aggctacacc atggtctatg gcatcttagg
     9001 gctcatcaat ttcgcccacg gcgagatctt catggtcagc acctacttcg ccttctatgg
     9061 gacggccgtt tttgccctcc catggcaggt ggcattcgtg ctagccatcg tcctcacagc
     9121 ccttttggga gtcgtcattg aacgcgccgc ctacaggccg ctcagagacg cgccccggat
     9181 ctccgtcctg atctccgcca taggcgtctc cttcctcatg gccaacttag ggcttgtcat
     9241 attcggcggg aggccgaaga ccttctatcg gcctcccatc ttccagaccc caatcgaact
     9301 gggaggagtc aaagtagtag cgctcttctt cgtcatcccg ctgctcacag ccatcctcct
     9361 cgtcatcctc aactacatcg tatacaggac aaaaacaggc cttgccatga gggccgccca
     9421 gtgggatttc gacacagccc gcctcatggg cgtggacgtg gaccgtgcga tctccatcac
     9481 cttcgcgatc ggctccgcct tagcagcagt gggcgggatc atgtggggcc tcaggtttgc
     9541 agagataaac ccgaacatgg gcctcttccc aggactgaag tgcttcatag ccgcagtatt
     9601 tgggggcatc ggcaacataa gcggagcagt gctcggcgga ttcatactcg gcttcggaga
     9661 gatcatgcta gtagccctac ttccagggct cgccggatac agggacgcct tcgccttcat
     9721 cgtgctcatt ctgattctcc tggtcaggcc gacgggccta ctcggcgaca agcaaagggt
     9781 gaaaatataa tggcaggaaa gagcattttg aagccaatac tgaagatagc ggcggtctgc
     9841 gcagccctcg tcctcttctt cctacttctc tggtgggggc aagagcattg ggacgcctac
     9901 gccgttcgga tactcaatct ttgcgggatc tacatcgcca taaccttagg cctcaacctc
     9961 tgcaacgggt ttacaggcca gttttccttg gggatcgctg gtttcatggc agtcggcgcg
    10021 tacaccacag cgctccttac aatggcgcca gagataaagc aggcagtcta cttcatcgag
    10081 ccactaatat acccgctcaa ctcgattcag tggggctttt tccccgccct tctcatggca
    10141 gccgtcgtct caggaatcgt gggctttctc gtgggcgccc cggccatacg cttccgcggg
    10201 gactacctgg ccatcaccac cttgggcttt gccgagatca tccgcgtcgt ttttacgaac
    10261 atcgtaccaa ttaccaacgg cgccctgggg cttaaaggga ttcccccaca cacagacctc
    10321 gtctggagct ggggtttcgc tctgatctgt atcgcaatca tggcaagact catgaaatcg
    10381 agcttcgggc gggcgctcaa agccatccgg gatgatgaga tcgccgctga agccatgggg
    10441 gtcaacctct tctcccacaa atgcctcgca ttcgtattgt cggccatgat ggcaggcgta
    10501 ggcggaggac tactcggaaa cctcataacc accatcgacc cgaacatgtt caggtttccg
    10561 ctcaccttca cagtcctcat gatggcagtc ctcggggggt tggggagcat caccggcaca
    10621 gtaatctccg ccgtcctcgt cacagtggct atggaggcat tgcgggcgct cgaagaaccc
    10681 cgggtcatct tcggcctcgc gctcccggga attccgggga tgcgaatggt ggtcttctca
    10741 atcgccctga tgatagtcat cctcttcttt cccaagggat tcatgggcga cagagagatc
    10801 tcgctcgatc gggcaaagag atttgcaacg cggttgtgcc gtacaaatac gagcagacag
    10861 gggtaatgcg aaatgtccaa gaacacggtt cccgcactag tctgcgaaaa cgtaacaatg
    10921 aagttcggcg ggctcacagc ggtgagcgac ttcaatctgc gcgtgccgga aggagccctc
    10981 tacggattga tcggccccaa cggcgccggc aagacaaccg tcttcaatat cgtgtcaggc
    11041 gttctccgcc catctgccgg ggcaatcaag ctcttcggcg acgacatcac agggctcagc
    11101 ccggatcgga tcgcctatcg cggcatagcc cggactttcc agaacatcag gctcttccag
    11161 aacctcacag tcctcgataa cgtacttgtc ggcctccatc tcaggcgcga ggtcaatctc
    11221 gcacaggccc tacttgccct gccccagcac agggctgagg aagcccgaat ggcccagacc
    11281 gcaacccaac tgctcgagga agtcggcctg gacgacttct ccgactaccg cgccaacgcg
    11341 cttccatatg gccagcagcg ccgactggaa gtcgcccggg ccctggccac aaaacccaaa
    11401 gtccttctct tggatgaacc tgcagccgga atgaaccccg cggaaacaga aaaccttatg
    11461 cgcctcatca ccgatatccg ggaagagttt ggcgtaacaa tactcttgat agaacacgag
    11521 atgaaagtgg ttatgggaat ctgcgagcgc atgacagtcc tcgatcacgg cgagatcatc
    11581 gccgaaggca ccccggcgga gatccagcga aacgagaggg taatcaaagc ttatctgggg
    11641 ggcggagcag ttgttggaaa taaaggatct tgaggtctac tacgacggaa tccatgccat
    11701 caagggcgtc tctctgaccg tgccccaagg gactctcgtc accttggtag gcgctaacgg
    11761 cgcaggcaaa agcaccctgc tcagaacgat ctgcggatta aaaaagccct catccggccg
    11821 gctcctctac gaggggcata acctgctcga catgggcgtg acccagatag tcaaagcagg
    11881 cataaccatg gtccctgaag ggagacagat ctttcccgac ctcacagtca ttgaaaacct
    11941 ccaccttggt gcctattccc gaactgacca agatgagatc gcccgcgatc gcgagcgcgt
    12001 tttcaacctt ttcccgcggc tgaaagagcg cgctgcccag aagggcggga ctctgtcagg
    12061 cggcgaacag cagatgcttg caatagcccg gggcctcatg gcaaaaccat cgctcctgct
    12121 catggatgaa ccatcactcg gcctcgcccc catcctcgtg gaagaagtct tccgcgtgat
    12181 ttcggagata cactccgagg gcgtcaccgt gctccttata gagcagaacg cccaggccgc
    12241 cctggccatc gccgactacg gctatgtgat ggaatcagga accatcgtgc ttcagggaaa
    12301 tggggccgac ctactcaacg acgaagcggt tcggcgtgca tacttagggc aggatgtaac
    12361 ggcgtaggcg aggtccggat cgagtagggg aatctaccca attccgtctg gctctggccg
    12421 tgttccagac agaataaggc catgtttccg ctccgagatc cgctgcttcg tgctcgcctc
    12481 cactcattgt attcctattg agctccgctt ggcacggatt atgcgatcag taggtccaca
    12541 aaaaggcacc ctcagccacc tttcgcagga tatgtctaaa acctagtctc ctcgccagac
    12601 ccgctcggac ccgcggccag gatgaaacgc ctcgatgacc tggccaacaa ggtgatgtcc
    12661 gccagttcca gaggccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatga
    12781 aacgcctcga tgacctggcc aacaaggtga tgtccgccag ttccagaggc cctggtttca
    12841 ctggtcgaac aggcagacac aacggtgttg tcattcaccg gttaccgtgc aacgacattg
    12901 cctactccgt cttctcaggc gacatgtcta ggtgcgagcg cgcaatcgaa tctctccaaa
    12961 gcaaaaactg ggttccgctg tcgacagaac ccggggacaa aggccgcaat atgcagtttt
    13021 gtaatgtgat ggtcaatggt accccacgca gatcgatcag cgcatcgggc acatccaggt
    13081 ggagcctgtc gtctgccacg ccagaagccc aacaatgtac cccataaacg ccatagcaga
    13141 acttgccaaa gacttcaaag ccgatttcgt atgccctgcc catgttgacg gactcgtcta
    13201 accccgcaac cttactctgc ctctccgccc aattcagcct cgaggaagcc gtcatcatcc
    13261 ccaacctcag gctccacctc atgccacgca tctaaaggct gcaccgcgtc tacgtcgtcc
    13321 ctttcaggcc agcacatcct agagtagtaa cagaccctac agtggcggct ccactcaggc
    13381 ttcgtcgact ccaatctgca atccgccgtt ctcgcggcaa ggcgaatcag tctctcctct
    13441 gcctcagcaa attcctcctg gccgtattca agtgccacac gcacgttggg atggagaaaa
    13501 ttcagaaccg cagtgacccg tttcgccccc aaccccctgt gaactgcgag ggcatacgca
    13561 tccatctggg tgcggtagtc acgggcggcc agatcgatgc cctcgtcatc gagcctgttc
    13621 gtcttgaggt caacaacggt gcccaccatg aactcatcaa cagctatgta gtctgcaatc
    13681 cccgacacaa ccgcggcgcc caggtcgagc aggaacgcct gctccctcct gccttctgcc
    13741 gacgcttcag ggctatcaag gtaattgcgt atcaagggcc agagctcttc ttcggattcc
    13801 acaaacgccc gacccctaag cccgtacgcc cctagctgct tcttgaggag agtcatagcc
    13861 tcattttcat tccgtgccca ctgacataca gagtggacca ccgacccgcg ctgagccgca
    13921 ttgagcctca tctccatctc catcccgacc gatggcgcga cgaggctccc ctgaaccctt
    13981 gaaaggccaa gcacatactc gtagacgtac caacggggac acctgtcaag gcacatcaaa
    14041 gccgaaaccg acacctgaac gggatcatcc tgatcgggag caacctttac agggccaacc
    14101 agcctgaact gatctgcgac cgactctgct cgcagcactg ctgagcctgt tggcactgtt
    14161 gccgcctcct gggcgggctc taacgccagc cccgaaacat aaggcccctt tccggcctct
    14221 tctgccccct tgtcccacat gtagcctgca agaggaagca tcctcacatg caccaacgct
    14281 tcgctgccag gcccaagagc cacaacagcc tcgaatacct cctcagcatc tccacatcct
    14341 gaggccacat ctcctcccgc agccgcgaag aaggacccca caacagcctg gccatatggc
    14401 atcaaccatt caggagctcc atccagcgac ataagattgg cagacgcctc aagggtagat
    14461 gttagcacca gatagtcgga ggcacgggtg gaggccacat agaggcatct catgctctct
    14521 tcattctcag cgcaggccag ttcgtccgcg accagacttc caaatgacgt gtcacggtat
    14581 cggatctcag gcgagatcgg cacggcaata cccatgtctg cagcaaactc gaagatgggc
    14641 gtgttccaac tcccagttcc tcggttaagc tcgggaacga ttacgaccgg gaactccaaa
    14701 cccttggatt tgtggaccgt catcaccgtc acaacatctg cttcttcttc cacgagcgac
    14761 gcctcagcct ccttggcgtc atctatggct gcacgctcga tcatgtctat gaaatccctg
    14821 atcgacgtag ccgaccggga agcttccctg gccatttcag ccaatttatg gacattgcct
    14881 atggccttga gtccgtcagc cgtcatggca agatatggct cgaattgggt ggcatcgagc
    14941 actgcttgaa tcatcccgcc agggctcaac ctgccggcaa gccgcctcaa gctttccatg
    15001 cactctctcg cccgcccgag cttcttcctt tcgtttgcct cccacccgtc aaccgccaca
    15061 tccatatcgc aaaacgcgtg agcaaggccg cctgcaagag ctagacgcaa gagagactcg
    15121 tcggaaagcc cgaagaccgg atgtctgagc accgccgcaa gagacaggtc atcacatggg
    15181 tcgtcgatag ccttgagcat gtttactacc cagactacct ccggacggcc atagaacccc
    15241 ctaccggaga cgacgtaata cggcacctca taccgctcca aagcggcctc gaaattctcc
    15301 agcctgctag tggttttgag cagtacggca atatccttgt atctcaccgg ccgcgttccc
    15361 acaagctctc cggtttccct gtcgtgctcg catacgagct gctccccatc ggccaccatc
    15421 tgcgcgattc gcgatgccac aagatcagcc tctgccagtg atgcgcgtcc gagctctgat
    15481 ttgccgtagg taataacgtg aagctccgtc cggggatgag ccgaggcggg catacttcga
    15541 aacgccgtgg cgtcactagt ccagatatcg cacaaggctg ggctaaaaac gtaattagtg
    15601 agctgcacca aaggatcgat tgagcggaag ttctgtccca gatcctccat tcccgcatac
    15661 ccgccatcca tctgcgcatc gtccatcgcg cccttgaaaa cctccacttc cgccccgcgg
    15721 aaaccatata tcgactgctt aacatctccc actatgaaca gccggtttcc aggcggatcc
    15781 cccgcaagct ccatcactat ctcgtgctgc agactattcg tgtcttggta ctcatctacc
    15841 atcacgaact tgatctgctt cctcacccgt tcgccggcat cggtatcacg cagcagatcc
    15901 cgcgcagcca gctccagtcc ggcaaaatcc atggacgctc cgttgccccg caaccacaag
    15961 tacgtctcat ccaacacctc gcacgcccta acaacagtct ccacagtcga caccatcata
    16021 tcggcgtaaa cgaaattcgc agccttctcc cgcacctgct tggcgatttt atcgagctcc
    16081 ttcgcggtca ctacgctccg caccggctgc gctgtggaat ggatcttcag gaactcctcg
    16141 atgaattcgg gcgaatccca atccagtgcg caaagcaggc ccacatcggc accgcccgcg
    16201 gcaagcgtgt ccactatgtc gtcccactct tcagccaggc catccttgga ttcctctgca
    16261 caccgtctaa tcttctccga gctgatccgg gcaaactcct gcataggcct gcccgttgtg
    16321 cgcattttcc catagacata cgcaattccc ttcgccaccc tcgacacccc gaaatccgcg
    16381 atgagcctgg ccatgttttc atcgccctcg cggatcagcg catcgatggc ctccatagcc
    16441 gcctggtcta tgaggcgcga ggcatcgagc ggatctaaaa ccgtgaagtg agggtcaacc
    16501 ccggcttcca gcggatagtc ccgcacaagc cgggagcaaa aagcatctat cgtgcttatc
    16561 ctggcctgac ttgcaagcct gcgggaaagg tcgcgccaac gcccagcgtc ctcaccacag
    16621 gcctctgcct cagcctggcg ttggacaatg gttgaccaga cccgcccctt catctcggtg
    16681 gccgcttttt ccgtaaacgt aatcgccacg atctcatcca agtcagccaa gccagattcg
    16741 aggagataga ggtatctgcc cactaatacc cttgtcttgc cggagcctgc ccccgcggcc
    16801 acaaccattg atctgtcagt atcaaaaacg gctctacgct gagatggagt ccactcagac
    16861 atcttgccca ccccctttgc caaacgcatc gccagcatcg agctccacaa ccctgcaaac
    16921 atcagggaag ctgcaatacc tgcatgcttc ttcagaaggc tggggggaaa acatgccctg
    16981 ccgcgcaccc ctgtcgcacc tttcaacagt ctcttccacc agttccgcga aggcgcgcca
    17041 ctggtcatcg tcgagcttgc cggcgcgctt ccgtccagca ttgaagtgct ggtggtaacc
    17101 tgccctccaa acgccttcag ttctccctaa ggagggaact tggtagtacc acacccctgc
    17161 aacctccgct ttcagcagac tctcggctgc caatgcataa agcccgagct gaatgtcggc
    17221 aacctccttg atattcctcg cactgggcac tgtcttccct gatttgtaat cgtatatggc
    17281 aagagcgccg cctggtcccg catctatcct gtcgatccgg cctgtaacgg gcacccgacc
    17341 ctcccccaca gccaactgcc ggacagatga cccgaatggc cattcaagat gtattgggcg
    17401 ccatacccct cccgtagcct cagtaaactc caactccttg attacaaagt cgcgagacgc
    17461 cgcgatgatc ccagcaaagt aggcatccca gacagacgcg gggaccccgg agcgttcgga
    17521 aggagtgcgg tccaccacgt cagaggctat cctagtaatc tcttcacagt actgatcggc
    17581 cttgtttgga tccagcggct cgcctatgtg gcgactgata aattccttga cgatctcgtg
    17641 cagacatgtg cctttgtccc ttgcgtccac atcgtcgccg acctcatccg gcggctcgat
    17701 tcctagcacg ctttggcaga agaaactgaa cgggcacttt atgtattcgt tgagcgagtt
    17761 tgcagaccag ggccgcacgt caaaaacagt ggcggccatt ttttcgagaa gctcatcaca
    17821 tccaatgcac ccgcggaaat ggccataccc atcctcctcc gctatttcag caacctgccg
    17881 cctgagccac acttcagcaa gcttggcaaa ggccacgtcg cggttccgca accataaaca
    17941 tgccccggct tcgggcttgg gctcagcagg catcgacccc atcccggcgg cattcacccg
    18001 ccccatactc caaagagacc tcatcctcgc ctcccgcttg ctcgccacat cgtcccatcg
    18061 catgggaaag accgaactgg caggcaccgt ctgggttatt tcgtgctcat cggcatctga
    18121 aacgccgagg cttctcaggt gctcgtctac aaagaacgag cgtcgggcca gcgaaccatc
    18181 ctccttggcc gtagcatagc tcaagtagat cctctccgct cgcgagtgca ccagcgtgtt
    18241 gaatacatgc ctgtcggcct ctgctccgtt gcccatcggc tcaagggaca cgcccgccct
    18301 tgccaaagcc tgaacatcgc tctcctgaag catccacggc ctgcgcctgc gccgaggcag
    18361 catctgctca cccaaacctg atacaaacaa aaactcccga tcacacgaag ccgcgcggtc
    18421 caggcccatc acctctaccc cattaggcga aggcctgttg agggcgaacg atcttctgcc
    18481 tgcagcgaaa gaaaggaggg agtagaaatc ggtccaaccg gccatagaac tcgcagtcgg
    18541 ttcacgtgat gcatcccaaa catcccgagc attggatatc tccaaaagca acgaatggaa
    18601 cgtagcccat gccatccatt cttcagcccc aagcattgga tcaccaggca acgcaaagat
    18661 ccggtccggc atgcccaaac gcgcaagggc agccctaaag ccatcaacat aaccggccag
    18721 tcccgagctt ccgctcgccc ttctcacatc ttcagcttgc tctgcagcct ccgccaaaag
    18781 cgccgcgtac cgctgcccat cgcaatctcc cgaaggatca catttttcga gctttgcctc
    18841 ccagcctgca aggggcagtt ccaatccttt gaggttaata gcgcgggcaa tctgcccggc
    18901 cctgcgcgga ccgagcccta ggtaggggct ggccacaagc ggcataatat cgaacgggcg
    18961 cccgatcgca ccgagcagcc gggcgacttc aagacagtcc agtaccaggc tggactcgat
    19021 catcaaacgt ttgcgcgaga cagtggccgg cacctggaat tcatcgaggg cctcaaggaa
    19081 catgggcaca aagccctcgg aatccgcgct cgccacacat acttgtgcag ggcggactcc
    19141 ttcgtccact atgagccttt taatctcccg cgcaaccgca cacgcctcac gcctcggccc
    19201 cgcagcagca atgagacgcg gcggcgtctg cctagcggga agctccacac caaccactcg
    19261 ggcagggctc accgcctcct ccatggcatc gagagcatac ccaccgtgac ctggtggaca
    19321 acgcagctct atctcgacgt cgacgctcgc ctcggccagg gcagagagga gaaccgcctc
    19381 cagccccccg atccacgcgt acccggccac tcggacagac tcaaggccat gtcggctcct
    19441 gccttcggca gagcgaagag actgggcaat agcggagtac aagcgccctc gctcagctcg
    19501 gcccgtgaaa tcaagaagct cgcaatacct tctccagacg cttagaacgt cggcagcatg
    19561 cgcactcggg atccggttcg cattgatgca cggactgaga ttgtcgacgg catcatggcc
    19621 aaagttggag accatctcca tccccccgcc ggagccggtg atgagagctt tttccagtct
    19681 ttcaggatcg agccccgcgc cgtccatcac agcgaaaagt tctgccaggg atcgggccac
    19741 cccatccctc tccgccccct gtgccaacgg tcccaactcg cctgcgatct tgcattgagc
    19801 aagcgccgcc gagaccaaaa gttcacgttg gatctcgctg acactgccct tcggttcaat
    19861 atcgagccga cgcatgagga ctgttgccag ttgatcgaag gtgtagacgg gtacggcgaa
    19921 aaccgccccg gcgtctacct gcgtaagaat cgctcgccga gcccccgcgg cttcctgcga
    19981 cccccggaga acaagcgcaa atcttccaaa tcggcccgcc ttgacggcat caaccgcttg
    20041 gcccaggtaa ttcgatgaac aaggacatgt cctcaattcc actgcaccgc ctctgatatt
    20101 ctgctcctac tattcgccag catccccaca taacctctgt acttccccga ttccggcagc
    20161 taggagaagg ttacgccgac tgcggacaag tcggctggtt tcaaggatga gttggcagaa
    20221 gaggcccttt tgcctttgtc agcaggaccg ccaaggtccg tcactgaagg cttgaaagca
    20281 gcgccaacat ggctatcaga gccaccaccc ctaaaatccc cgttatcgcc tctcctgcag
    20341 tcatgtatcc ctgaatctcg acggcgtagt cgccagccat tatctcccct gacgactcca
    20401 caaccttgcc cctgcctaca gtcccgtgaa cggcctcaat tctcaccacg cccacctcgt
    20461 cagcggacat gaactgatgc tgcattcccg gatcgcctac ggaaatcaca ttggggcgga
    20521 atatccgaaa gtcacttcct cgggtaagca caccctcctc gtccgtgtaa atatagacct
    20581 ctttcttgct ccggaactcg agcaccctcc cgacaatggg aaaagcggaa gcgatcttcg
    20641 tctgcaagga agagccaaaa cacgatctta tcgcattcat acacgcagcc gacgtagatg
    20701 aattatcgct gcctgtgcca gtcacccgaa aactcgaaag aatggcccct gtagacatgt
    20761 cgatgatcct gatggaagcc tctgcagatc cagcgtaata gtaatgcctc ttgccgtcct
    20821 tatccgtcca aaactcgctc ttgcattcgc atctgtcgag cgacccgacg aatgcataat
    20881 caatgccgag caactcgccg agccggaccg cagtatcagg atttacagca cctgacacct
    20941 gaaacctttg ttcggagagt atgcgctcta tgtgctccct ctcaacaacc tcgaacctct
    21001 tgatgcccac cagcgtgtcg gacagacaat tcgatgcata ctgaacgaac tgataattga
    21061 aagaacccgt gaaactgata atccccaccc tcaggcggcc gctagcaggc acctccgccg
    21121 ccccgatgca aaccgtggag ccaaagacta ttgccgccac caaaacaacc atggcaacag
    21181 ccctcaccct gagcttctcc ttcatttcac gcacctcctc agataacgca atggcattgg
    21241 cctgcccgtc gtcagccctc cacacttgtc ttttgaaacc atccctcagc ccggcaacac
    21301 cgcaaggcgt ccggaactat tcccaaatgc tatctatcat gaacctgaat ccagtggcca
    21361 caattagaga gtgtcacatg tctcagcgct cgtcagcagg gccgtaagag cccacattcc
    21421 aggcgttgca atgtccgcgt atccagcacc aatgaggctg cctaatccag ggcacgcagc
    21481 cctgacagag tgctccgcgc ccacgctccg agaaacccga atacctgccc ctccatgatg
    21541 tccccgtcgt cggtgtactg atccgaaagc gccgccaaac cccaagccct gtcggactgc
    21601 tcgtctattg actcccgcac ccactctgcc cgggatacgt tttgcgcctc cacgaggtta
    21661 ttcacattgc caatagcatc ctcaaaatcg aacgtgagct cgattagctc cgccatgagc
    21721 tctcctaact tctcttcgtc ggctactccc tgcgccgccc acgacaaagt tgaacacaca
    21781 agcattactc cgacaacaat tgccgcacag tctgttccag gcacctacga ggcttcaaac
    21841 gatcccctcc gcgtccattt atatgcctat gttacatatt acccacctaa gcgcccatac
    21901 ctccatagat tcgcaacggc cagtcagcga tcgaccagcc tcgccgggtg ccatccggcc
    21961 tgctttacca cacgaggttt tgtttgcaag caagtctcga taagttcctg cgcgatcccc
    22021 gagtttgcat tgcccgcaat tgagagcttg accacttgat tgggccgaaa cgccatttgc
    22081 cgattgccca gggtctcgcg cctacaaact gaagcagcat caatgatgaa agttacggtt
    22141 cagacaggac agatgaagtg aggtgattga atggatatca tacgcctttt ggagtgaggt
    22201 gagaggtcct cgccatttgc ccacagcaca atccgccaag ctccttccgt tgattccgca
    22261 atcgctttta cccgcgtttt ttctccttct cccacgtctc cttctcgatg cactgatgct
    22321 ccatcgtaaa cctgcgggag aggatagcaa attaccgcca gtcagtgcat cgatgtaatc
    22381 tcgattctca aaacttgtct ccctaaacct gtcacggacg agtttagtcc tgatctcgct
    22441 gtagtcatat gtcaactcct gcaccccttt ttcgaggccg tattgagccc gcccgattct
    22501 gcttttttgc tgcagacggg cctctcctgt attgggcata gtccttcgcg gaaataacgc
    22561 cttcacaata tcttcccgaa gtcactcatc gatcttccac gtcacgctca gcatgaggtt
    22621 ccttgcccgt ccggagagga tcagacgcca ctggccgcgg gcagctgtct caaacgatat
    22681 ctttggggtg ctcaccctgg ccatcttcgc attgtattcc tcccgccaca tcacatcgtc
    22741 atttggccca acgagggaga tctcgaaatc cgccatcata gaccaaaaac tgatactaat
    22801 ctcgagagcg ggctgcaccc cgtgcacctt gccgacatca atagctacga cttcaaaatc
    22861 cccatcgaga tcccgcagcc tcacagatga tatgtgctgt agctttgcac cagcgggcaa
    22921 cggaaccgct ccatgccgga agatcagctc gcctgcgacc gcaaagactg caataacgag
    22981 tactatcagc aacaccgtcc agcccaacac agaccctgct ttgccccttc ggggccccgg
    23041 cataggtttt tcgcgattct ccgtctgcat tttttacccc tcccgtggat ctatacggca
    23101 aaacctcatc catccacata tcgaccggca atgcccaaag acaaccaggt gccgccatgc
    23161 ctgtctccat aaacacaccc ggcccaaccc agaatcgaag atccccaata tcagccgccg
    23221 accccttagc cgaatgggca gcgaaatcgc aagcctcgta tgaccgccaa gtgcccgcta
    23281 ccgctggctt ctcatcccat tatgcacccg taatggttcc acatgactca attttatcct
    23341 ttgcgatctt tctcctcctt actatcttat gtgtaccccc gaatcccgct cacttctaac
    23401 cagtccattg gcgattccaa cgcactcctt cgaaggagag gagtcctcgg cgccgaatac
    23461 ctgtgcacgg gggtgttaga gttggatttg aaatggaggc ctcgaatgat agaacccggt
    23521 agagctttgg caatctccat cgacttccag gacaaaattc tggccacagt cgaagaccgc
    23581 gatgagatca ttgcccaggc taccagtttt gtgaaagtgg cccgcctagc cggcatgccc
    23641 gttctgcgct tggagcagaa tcccgatcgt ttgggcacca ccaccgatct catgcgggat
    23701 gctcttgggg attcacctgt ctttccaaag atgaccttta gcgctctcag atcccctgag
    23761 atcgaatcag ctgtccgcca aagtcaagct gagcaattga tccttcttgg tgtggagacc
    23821 catatctgcg tggctcagac cgctctcgac gcaatggcca tcggatacga ggtgtacgtc
    23881 ctagcagatg cctcatcctc agtaaaatca ttcgacagga agaccgcaat cgaacggatg
    23941 cgccacgcgg gtgctgtgat atctacagca caagcagcgg cgttcgaaat agtgggggcc
    24001 gcgggaacgc ctctgttcag gcaggcaaaa tctcttttaa aagagatgta agcagtagcg
    24061 ctcggttgat tcagctagtc gcatcgggat agaatgggaa acgggggcag gctcgtccct
    24121 caccacctta ttgcactact catcaaggca tgtgatagat ctatgcaatc tatggggaaa
    24181 agctatgaga taaaatcatt ggccagaggc gctatgttcg gcgccatcac agcagtactg
    24241 gcccacatct cctttccagt cccattcagc cctgttccgg tctcgggaca atccttcgga
    24301 gcaatgttgg ccggcctgct gctttcaccc aaagccgcgg cagcaagcca gctcgtttac
    24361 ttgctcgcag gcgcagtggg gctgcccgtc tatgcaggag gccacgcagg catcggggtg
    24421 cttctagggc ccacaggagg gtacctttgg ggaatggcgg caggcgccta tgtctcctcg
    24481 tggattgtta agatgcacat ttcaaaaggg aacgggctga catggcaaaa atgtgccatc
    24541 gcctcagcaa tcggcggtgt ggcagtcgtc tacatcttcg gagtcgtcca gctgagcctg
    24601 gcaacacaaa tgcctctgcc gcgtgccttc gtcgtcggcg cgcttccata cctcccaggc
    24661 gacctgctaa aggcggcctt cgcaggcaca ttgggaaacc gtctggcaac ctacagaata
    24721 tagtctgttt accgcagcca ccccataaaa acaatcctat taccgggtgc agacccgaac
    24781 catctcatcc gtgcccatcc actaaacatc gatgactctc agctcggcag ggcgcccctg
    24841 ccaatccagt acgctatctt ccgatatgac ggacccatgc atgtcgctcg tcctctcgca
    24901 acaagagctg ccgcctctcg ccgccaagcg cccacaaaca gtacgcatca tatcccggtg
    24961 ccacagcaaa agtgtggtat ccaaatggaa tagccaacgt atctccgttt tcaatcgtgt
    25021 acgactggtc gaaccgacgc ttcggagagt agacccggcc cataccgaat cccctctcgg
    25081 gcctgaagcg aaagtggcaa acctcctcca gcctcgtctc ctcgccaaga acatcctcat
    25141 catgcttgtg ccctggatat gcaagccagt tgccccggca taccatagat tcccccacaa
    25201 tcagccgtcg tgcccctgca gaagtctttg cacccacaat ggtgctgaca gtccgctccc
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcccccagc ttcctgcaga
    25381 caacctcgtc aggcgagatt atctccacgc cacagtctaa cctgagctct cgtccgcaaa
    25441 cagtttcctc agctcccgct cgcatctcca tggtttcaca accaccctca acacatccgc
    25501 attcgtacag acagtcaggc ccaggctcag gctgctccgc cctcacaagc gccatctcca
    25561 caaacggaga taatcccttc acttcgaacg ttgtgaacgg atgggcaaag aatgcccacg
    25621 gagggccgcc gaagacatcc cttctgccgc caatcgtctc acgccgctcg acaccatcct
    25681 cgtcttccca ggccacctcg gcaacaccgc acaagatgac cgctacagtt tcaagaggac
    25741 cgaaattgaa ggccctgtcc tcgtcagcct ccacactgac cattccgaga tgaacgtagg
    25801 ccagccccag ctggggatct gtaggacttg cgatctccct gaccccagtg acgctacatg
    25861 ctctatcagg catcgtcttc ttaagcgaca cgtgcgcacc tcctcgtttc cattacgggc
    25921 aacgccaaga tattcttcgt acagatggcc tttactcctc gtcagggcgg agttgtatta
    25981 taatagaaca aacccctcaa acctaacgaa agggtagagt cctgcaatga aaagaaaacg
    26041 gatcaccacg attatagtat caactttgct gatctttgtg gcaagctccg tctgggcaaa
    26101 ttctgaagac gacgcctggc gcaagaagat cacagaattc tacgcgtacg atacagcgca
    26161 gccctttgaa atctccttca cgttgctcaa cgacaatcaa tggtatctcc attacgatgt
    26221 gtcgtacaag agcgaaggcg acacagtcta cgcccactat ttcgttccag taaccgctaa
    26281 atcccagcct tgtccatgta ccatagggtt acacggggtc ttctccgatt ccgaagaaca
    26341 gttttggatg atggcggact tcctggcaaa gcggggcatc gcggccctcg cacccagtct
    26401 gccacagcac cataggagag ccaagggaaa acagttcgta tccggccaaa gattcgtagt
    26461 agggactccg gaaaccatcc gggacaacct gaggcaagca gtggtcgacc agcggcgcgc
    26521 ggtggactgg ctcagcatgc gtccggagat caatccatcg gcaatctcca tcgcgggggt
    26581 cagcatgggc ggaatagtcg gttccctcac ttacaagata gagccgagaa ttcgcaacgg
    26641 cgttttcgtt gtcgctggat cgggtgtgga cggcatcgtc gacaacagcg acctatcagt
    26701 cgtctcagcc ttccgccagg cagccagtgt gaagcttttg aatccccaga agttcatcga
    26761 caccctccag atagtggatc ccataaacat ccccgacctg tatcccaggc cagtgctgat
    26821 gatgaacggg acatcggatg cgatcataac tccggctgaa gcgcttcgtc tctataacac
    26881 tttgggcaaa gcagaacaga tctggaccaa tggaagccac tattatccgg cctatgctgg
    26941 cgaatatctc acagcctcat tcctggcatc gcacatgccc aaatcgtatg gggatactcc
    27001 gcccgggtca gtccttgtag ccagcccggg ttatagattc atcgaaaaag tccaaccccc
    27061 cgttgcaggc agcaaccgcc tgtgggttga ccttaaagtt cagattgccg aaaacagcaa
    27121 ggaacttcag cacaaagtgg ctgtgcccct agtgaacagg atggttccgg tgatcgtggc
    27181 aagccgcgag acttatcagc gaatagaaag ggacataatc aatctgccgg cattcatcta
    27241 tatcatggaa tctgatgatg cctctcaatt cgcggcggct ctcgcctata tcgccgcgct
    27301 cgatctgacc gccaatccac tggtgtacta tgcagctccc tcccaaggca ctagttgcac
    27361 actgacccag ttcacagcgg ttcaggttga gtcggcaaga cacatactcg cctcgatcct
    27421 gtcggtcaag tcgagtgggg caacgcccgt gtctgtgcct aaaggacttg gatcattaat
    27481 tgctcaatat cctaaccatg ctcaagcagg cattgccctt ggcaagatga tccgctacga
    27541 gaacctagga ccgctgccgt ggctcccaac ctggcaagac caaggcgtta ggtggtctct
    27601 agggccagag gagccggacc aaccaaaaaa catacgccgt gaggacaagg ccgggatcgc
    27661 accctggaca aatagaagta acaacgtgta acctcccaca cgcaatcaag gagagggcgc
    27721 cggtcgccca accaacgccc tccccctgat tgcgcccccc caggcctgtt acccgcaacg
    27781 cgcacccgac tcagcctctc ggtcctcgct tcgtctgccc tcctcccaaa ggcatcgttg
    27841 tccttggttg cgcatggcaa cgtctgtggc cgcgttcggc aatccggcgt tcaccctatt
    27901 gcggattgta cactccgtgc tcactcaaat agttgaagag tccttcgcca tgttgctgtt
    27961 cttcttgctg gatatgttgg agagcctggc gcacgttgga gtttgcggac tcgaagaccc
    28021 ccgtatcata cgtactcgaa acgaatttct ccgtagccaa catgtccatg accaatgtgg
    28081 catcattctg gttccgcagg cccgtcattc catggttggc agtttgactc gcatgttggc
    28141 caatagtccc gacttccatt gaataccggc ccactcccgg accgccctga atcatctctg
    28201 tcccgccctc gcctgcgctg ataatcatct ggttcccctg gagcaactgg tttatggtgt
    28261 catagtgctc ctgctcctgg gacgcatact ggctgaacaa ttgcttaagc tgtggatcct
    28321 cagcctgctg agcgtagttg ttgtatttct ggatgcacag tttctcgtgg tttaactggt
    28381 cactgagcaa agattgctcc ttttgcgtga gcttgttctt caaatacacc acctcaccca
    28441 ccagttttgc acaggcaaga gtggtttatg cgttagaagg cggcacaccg agcataatcg
    28501 gggaaacagt gcggcgggcg ccacgtattc tgcagagcgc ctctcattac ctaaatcctg
    28561 caatataact gatggccgcg ctctcataag cccgccgagc ctcccaggcg cccttagcta
    28621 ctgaatcgcg tctgaccgtt tcttccagat tattactctc tttacgtgtg atctcaatct
    28681 tcacatcacc gatcatgagt atcatctctg cccctccaac tctgagcacc acatttatca
    28741 atcttattca taccgttgtt aatatcataa aggtacttat ggacaatgtc aatatctctt
    28801 ttgataatcg ttagccctaa cgttgctctt tcatctgcga acatgatatg accttcatgc
    28861 aaccggcaca tgccaggtgc aacaaggaaa tgggcctaag gaggattgca gtcatgccag
    28921 gcgaaatgat atgcactagt tcctcaggca aaggccaacg cgaaaccatg attcgcatgc
    28981 ctgaaggccc taccagctca gctgagagat taccacacag gaggtcgcat caatggccat
    29041 tgatactccg aattggatca agagctcagt catatacgag gcatttcctc gaaaccactc
    29101 gaaaagcgga acattcgccg gactctgtca cgacctcgag agaatatcac gcctacatgc
    29161 tgacgttctc tggctaatgc ccatccatcc cataggcgca gccaaccgaa aggggagcct
    29221 aggcagtcct tatgcgatca gggactaccg cgccatcaac cccgacctcg gtgatgagca
    29281 gtcctttcac gaactcatca acaaagcaca tgccttgggc atgaaggtaa taattgatgt
    29341 tgttttcaac catacctccc gcgatagcgt tctcctcgct caacatcctg agtggtttct
    29401 ccaggatgac catgggaacc cggccaccaa ggtcccggat tggtgggatg tatacgatct
    29461 tgactacagc caacagggcc tttgggatta ccaaatagag acactccgca agtgggtgca
    29521 gatgggcgtg gatggtttcc gctgcgatgt agcgtcactg gtgcccctcg atttctggct
    29581 cacggcccgc aaagagcttt cgcgcgggcg gaaggagaac ctgatatggc ttgcagaaag
    29641 cgtcgacaag tccttcatca agatcctgcg cgataccgga tggacggccc attccgaccc
    29701 cgagcttcat gctgcattcg atctcacata cgactatgac ggatttgatt atctcaaggg
    29761 gtacatccaa ggccagtgtc gactcgacgc ctacctcaac catctttaca tccaggaaac
    29821 cttatacccg gcacacgcag tcaaaatgcg attcatcgag aaccacgaca acccgcgagc
    29881 cgcggagatg atcagggaca agggatcctt aatgaactgg acagtcttct atacgctgct
    29941 tcccggcgca accctggttt acgccgggca ggaaatagct tccgcacact atcccgacct
    30001 tttcggagct gatcccatag actgggcaaa cggcgactac gaattcacgg ccttcatgga
    30061 aaacgcactt gagctgtcca agaagatcaa aggcacttgc aaacattttt ccgtctccca
    30121 gctcgtggac ggggtagtca agatagtctg gcgtggaagc ggttcttcct acgtcgccct
    30181 gatcaatctg gcaggcaagt atggagaagt ctgccttgat gaactgaagg aatgccacgc
    30241 agaggtgtgc ttaggcagaa gggaaaaggc gcaagaacac gggcgctgcc gactcgcgaa
    30301 tacgccactg gttgttagaa cgcctgcctg aagcgttccc ttgaggcaag ctttgatgga
    30361 gcgcctaacg gctccaaaga actgagcatc aatcgggcaa ggcaggcctc ggcatcgtaa
    30421 gcgatgttga caaactactg gtcggtgcag aatcaggtcg tgtaggatcc gtctcttttc
    30481 gagtctggca gcccatactt tctctgccaa agcagcctgc agctaggccg accgcacaat
    30541 caatatcgag acagacgcgg tgtacgaaac gcacccccct tcagcaaaca cagctaggag
    30601 gcgaaatcga tgctccgtgg caacagagtc gttcttcgcg aatttcgcaa agaggatgca
    30661 gggcgcatcc aggaatggat aggtaatgag aacataacga agtatctggg attcatgatg
    30721 ttcccgcaaa cattggaaga gaccgaagcc tacatcgagc agcagctccg caaggagcac
    30781 gtgccgagcg agggccagtt tgtgatagcc ctcgccgatg acccccagct ggagtacgta
    30841 ggcgcagtgg gcttgcacca aatagactgg cgaaaccgca actgcgagct tggaattgta
    30901 ataggccggg aagaaatgtt gggacagggc ttaggcggtg aagccatcga tcttgccctc
    30961 ggtttcgcct tcgacttcct caacatgcac aaagtcaacc tcactgtatt cgaatacaac
    31021 gaacgtgccc atcggtgcta cctcaagtgc gggttccggg acgaaggccg cgtacgccag
    31081 caacgctact acgacgggca ctactgggac atgattctca tgggcatcac gaaagacgag
    31141 tatgttcagg cacgccgcga gaataccaca aacgtctgct atatctaaga agtagcataa
    31201 gacggacagc ttcttcagct aacggcctca gttaatgacc ttatgcagag aaaatgatgt
    31261 ccttttatag aagcaatagc cacaggccca tttcggctcc cacgcaacaa gagcaactct
    31321 caaccagcat ttgagcagtg ccaatgtgat ggggctgcgc tgcactatct gaagttcaaa
    31381 cctgaggtcc cataaaggcc ttcggcgctg cctgatccgt gccggcgcag gagtcaatcg
    31441 gaggctgatt cgtccttccc atgcacctag cagccgcact aaaaccatgt ggaagcatca
    31501 tgttgcaaca cgaacgcaag agcaacttca tggatgagcg tgaaaagtgc gcagcaaccg
    31561 caacaagaac gattcacacc gacccggcca tttgcgatct cttcctccaa atcaggatcg
    31621 tcaccgccgc tcgttcccct cagccgcctc catctcctgc caggctggag gcgccgcggc
    31681 tattcagttg tcttccctgc aaggcaacgc cataggcgaa ggccgtcact ccacatgtca
    31741 gaatacccta tgagccaaga cggatacgtc acgggcaata tcgaagcagc gaactgtatt
    31801 atcgccgcaa ccatcccggc ctgcgccagg tcgcgcatga acaggggccc cgcagcgaac
    31861 atgctcttca ctacgccaaa aagcaatgcc gcaaggatcg atgccaaagg accggccaac
    31921 atgatccaaa caaagtgcgc ccgcctagcc tccacaacac ctgcagtcca tccccagcgc
    31981 ccgacccatc cgctccaagg ctgaagcaac acctccagcc tcccccgcct gaccagcgaa
    32041 atcttgggat ccgaccccaa acggaccaca acaccatgtt cagggacgag agccaaggcg
    32101 gctgcagcat ggccaagatt atggatgagg gtgatcagcg gcactatagc agcccagaaa
    32161 atgatgatgg atacaagccc gatcatctcg atgtccctcc ccaggccaga atgatcacca
    32221 cattctattc gccgggcgtc ttgcatatca tgacccatcc ttcgctgcgg actgcccgaa
    32281 agcagactca aacgggctat gcgccgctgc acggagcggc atgcctgcag cagacagtcc
    32341 ttactgcctg agcagcccgc tcaacatcgt cagctgtgaa atgcctgttg gtcacggccc
    32401 tcagaccatg gggcggcctg gtgctgacgc ggaccccctc cgcgctcagt tcggcagcga
    32461 actcgttaga tccgatcccc aagcgcgaaa gatcgaagac caccatatta gtctctacag
    32521 tcgaaagatc gatttcaatc cccggcacgt ccaccaaagc ttccgccaac gcgcgggcat
    32581 ttgcatgatc ctcggcaagc ctgtccacca tcttctctag cgccactatc ccggccgccg
    32641 caagcacacc ggcttgcctc atccctccgc caaccatctt ccggttcttc ctcgctcggg
    32701 cgatgaagtc agcgtccccc gcaagcatgg agcctacagg cgccgacaag cctttggaaa
    32761 ggcagaacat gaccgaatcc acgtgacagg cgatctggga tgcatctacc ccaagcgcga
    32821 tagcggcatt gaaaatcctg gcaccgtcca tgtgtatcga aagcccgcga tccttcgcaa
    32881 catcgtggag agctgcaagg atggttagat cagtcacagt ccctcctccg cgattgtgtg
    32941 tattctcaat ggcaaggcac gttgttggag cgtaatggat attcgattcg cggatcgcag
    33001 cccttacatc gtctggatcg agcacaccgc tattacccaa gacaagccga ggcaccgctc
    33061 cggccacagc cgacaagccc cccacttcat agtagtagat gtggctttcc gcctcgagaa
    33121 tgatctcatc ccccctgctg caatgtgtca tgacagccac gagattaccc atcgtcccag
    33181 acggcaagaa cagggccgcc tcctttccca ccttcttcgc agccagctct tcaaggcgga
    33241 ttacggtggg atcctcgccc caaacatcat ctccgacctt ggcattgcgc atagcgtctc
    33301 tcatctcttc agttgggagc gtaagagtgt cgctcctaag ctcaatccat tccatcttcc
    33361 atgccaccct tcatatagcg tcttacaaag ctcttcattt acctggaatg atacttcata
    33421 cgccgtccca ttcctctatt acactcgaaa gtcaattccc atcttttatg ttttagaggg
    33481 tgccaatatt acatcggatt ttccatccaa cccacccagc tcctagcccc aaacgccatc
    33541 taagtgtata atgtaagcgg tatccatatg tcgccgcgca catgcgcggt ggcttctcat
    33601 gtatcagctg tatcacgccg agatacaacg gcctgttcga acaatgatgc gaggtgagtt
    33661 tatgtgggcg ataacgacag gctccgcgag ttgaaggccc aaagagaaaa agtcattcag
    33721 ggaggcggtc caaagaggat cgagaagcag catgctagcg gcaaattgac cgcaagggag
    33781 cgaattgagc ggcttctgga tccgggcagt ttcactgaac tggacatgtt cgtggagcac
    33841 aggtgccagg aatttggaat gcctgagcaa gaactgccgg gcgaaggggt tgtaaccggc
    33901 tatggaacta tagacggtcg caaagtcttc attttcgctc aagacttcac ggtaatgggc
    33961 ggatccctcg gggagatgca cgcaaagaaa atctgcaaag tcatggatct ggcaatgaaa
    34021 gtcggagcac cggtcatcgg catcaacgat tcgggaggag cgaggatcca ggagggcgtc
    34081 gacgccctgt cagggtatgg gcagatcttc tacagaaaca ccatagcctc aggcgttgta
    34141 cctcagatct ccgtgatcat ggggccgtgc gccggcggtg cagtttactc ccccgctctg
    34201 acagacttca tattcatggt tcaaggcgca aacatgttta tcaccggtcc acaggtaatc
    34261 aagacagtga cgggagaaga ggtcagcgca gacgaacttg gcggccccac tactcacaat
    34321 accgtgagcg gcgtggcgca ttttatgtat gacactgaag atgactgctt cttggctatc
    34381 cgaaaactcg tctcctatct gccgtcgaac aatctggaag atccccccgt tcaagccctg
    34441 gaaaatgcat cgtcagccga gatcatacct gaactcaggg agattgtgcc tgaagagtca
    34501 aacagacctt atagcatgca cgatgtgata ctccgctttg ttgacgatgg cgacttcttc
    34561 gaggttcacc agcattatgc caggaacata atcgtgggtt tcgcacgcct aggagggcgg
    34621 ccaatcggca tcatcgcgaa tcagccgcaa gtagctgcag gctgccttga catcaatgcg
    34681 tccgacaaag cggcgcggtt tatcaggttc tgcgacgcat tcaacatccc cataatcaac
    34741 ctagttgacg ttcccgggtt cctccctgga gtcaaccagg agaccggggg aatcatacgt
    34801 cacggagcca agcttctcta tgcttactcc gaagccacag ttcccaagat cacattgata
    34861 gtccgcaagg cctacggcgg agcatatctc gccatgtgca gccgcgacct cggggcagat
    34921 gtggtctacg cgtggcctga tgctgaaata gctgttatgg gcccagacgg ggcagcaaac
    34981 atcatcttcc gcaaggagat tacagcagcc caagatcctg aggctgccag gaccgaaaaa
    35041 atagccgagt acaggtcgaa gtttgccaac ccgtatgccg cggccaaacg tggatatgtg
    35101 gatgatgtga tcgatccggc agaaacccgc cgacggctta tggatgcatt ggacatgctc
    35161 tcaactaagc gggagactcg acctcccaag aagcacggga acatccctgt atgattgggg
    35221 gaagattcta tgactgtagg tgaagaacta atcctcggcc tgcaaaccct ggttctggga
    35281 atggtggtca ccttcatcgg tctggccatc cttcaggtca taatgatcct aatgggacgc
    35341 gcctcagatc gcaagcgcgc acccgcggga tacacagtcg gggacagggc tgaaaccgag
    35401 atacgcgacg aaatcacagc gtcagacgat gaggagctaa cacatcgcgg ttccgaattt
    35461 gaagggcgcg cagaagacta ttcagaagag caggttgccg cgatcgcggc tgtaatggct
    35521 atactgatgg aagaaggagc agccccggtc catgtaaaat ccgttacaca ggtaggccca
    35581 gtcgccgcga acctctgggg gcttgccggc aggcaagatc tgatgtccgg acgatccagg
    35641 gcataacggg gatctaaata ccaagttcaa tccgatttcg agggggattg ttcaagtgag
    35701 aaggtttcgg gttcaagtca acggaaccac ttatgaagtc gaagtagaag agatcggcgg
    35761 aatgggaacg ccggcggccg cccaacccgc tccagcagcg ccgactccca gcgcccctgt
    35821 gtccgcgggc cctgcaaagc ctgcagcttc caaaaagccg gcggcatcag gtcgcggcac
    35881 atcagtcacc gcacccatgc caggcacgat aatcgacatc aaggtcaagg tgggcgacaa
    35941 agtcgaaccg cggactgttg tggcgatgct ggaagctatg aagatggaaa atgagatttt
    36001 tgcgggcaga tcaggcgtcg taacgcatat agcagtagag aagggggcct cggtcaacac
    36061 cggagatctc ctagtatcca tagacccggc ctaaaggagg gcgactttca gtgcaagaag
    36121 cagtcgtcga gttcctatac agtacgggct ttgctgtcct gacatggaaa gaactcgtca
    36181 tgttcgctat cgcgggctgc ctcatgtacc tggcgatagt aaagaagttt gagccgctgc
    36241 tccttcttcc gatctctttt ggaatgctct tagcaaacct gccggctgct gggctcatgg
    36301 agggtccgca agacggcatt gttggaggat tgctctacta tctttaccag ggcgtcaagt
    36361 taggcatata cccgccactc atatttctgg gcgtaggcgc aatgaccgac ttcggtcccc
    36421 tgcttgccaa tccgtctacg atcttcctgg gagccgccgc tcagttcgga atattcatca
    36481 cgtttctcgg cgcactgctc ctagggtttt cgccagcgca agcggggtcg atcggcatca
    36541 tcggcggcgc agacggccca acagctatat tcgtcacgag caggctagca ccggaacttc
    36601 tgggcccgat agcaatagct gcctactcct acatggcctt agtgccgata atccagcctc
    36661 ctattatgcg tgcccttaca cgcaaagagg atcgtcaaat cgtgatggag cagcttcgtc
    36721 ccgtatccaa gaaggagaag atactcttcc caatcatagt gactgtcgtt gtaggactga
    36781 tgctgccatc ggctgcagcc ttggtgggaa tgctcatgct gggcaacctg ttccgcgaat
    36841 ccggcgttgt agacaggctt tcaaagacgg cccagaacga gctgatcaac atagtcacca
    36901 tcttcttggg gctcactgta ggagcaacag ccagtgccga aagctttttg acatggcaga
    36961 ctataagcat aattctcttg gggcttgtgg cgttctcagg gggcacagca ggcggagtgt
    37021 tgctgggaaa ccttatgtgc cgacttacta agggcaaggt caacccgctt atcggagcag
    37081 ccggcgtatc agccgttccc atggcggccc gcgttgtgca gacagtggcc caagatgaaa
    37141 acccaggcaa cttcctgcta atgcacgcca tggggcccaa cgtagcagga gtcataggtt
    37201 ctgcaatagt cgcgggagtt ctgctgtcta ggtttggata gacgagttga catccgtgat
    37261 gagcaggtga ttggataatc gcaccggcat gtgccaatgt tcagatgggc agacagaact
    37321 cgtcatcccc cgttcctccc ggccaataca aggccagact gacgggggat gttctcttct
    37381 ctctccggag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcccggcca
    37501 atacaaggcc agactgacgg gggatgttct cttctctctc cggaggtggc caaaggctct
    37561 tctggcttcc accgtgttat gcggtcgatc tcacgtacgt gacagtccag gtcagagttg
    37621 agcgccgaca cagtcttgtg catatcgcag accaactcta gcgtccgatc tgcatccggt
    37681 tgaaatccgt agatgttcct gaacacgtac ggaacctgtc gagaagcgca gctgtgccgc
    37741 tggacaaaag cggcggacag agtccgggga ttcatctgct ccagcaactc tctgtgccag
    37801 tcatgcccat caatcctcat agccacggtc ttggcgatgt tctccacgaa cgtgtagaaa
    37861 tcgtggagca tcgatccgag tatgcgcaat gcctacaagc ggaaaccgcc acaggataga
    37921 tgaggtgact aagattctct cgttccttgg ctattcgggc ctgaatcacc aggtagtcct
    37981 ctggtctgaa atgcagatcc ctctttcccg cgcatgctca aagggtcggc tatctcgtca
    38041 gccatgacgt acatttccca gtaatcgccc ctgaaccctt caactaggat gcaaccttct
    38101 gcgagggatc catacagcgt gaccgcaggg gccgaatagc gagttcctag aagatcagca
    38161 atacgacgcg ccctttccat tgcatcagcc ataacaagct cggcgcgttc ctccgaccac
    38221 caaaataccg cctcccttat ttcaggcaca ttccatccat gaatgccgtc atcgaccatg
    38281 gtcacacccc ccaacaaaag ctgtagttca tccacaagct gtggcgcaaa ctgcggaagc
    38341 actccactca cgcccactca gccaaatccg tgctcccgca agcaggttgt ccccaccatc
    38401 gaagcaattt ctgcatcgaa aagcggcctc gcagagatta cgcccacgta tcctgcgacg
    38461 agatacgtaa cgttgccgct actatgccag gtttgtggga gaacatcgct ttgcccaaat
    38521 caccgcagcc tcaggcattt gcgaatgcga gcagctgcag caacnnnnnn nnnnnnnnnn
    38581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38641 nnnnnnnnnn nnnnnnnnnn nnnnacactc tggaaggcta gatccatcca cccgagcgcc
    38701 tttcgcaaaa aagcaagtcc cctgcctcgt gatgcatcac cagggcaggg gactgcaact
    38761 gctccagccg cgctcgattc acttggaaat cgcaggcttt gccgctatat tcccagaggc
    38821 ggcggcccga agctgaagga tagctgcaca aagggcagtc ccgcgctctt catagggcca
    38881 tcgaatgaat ccttgaaggt catcccatcc atccatccgg tcgggctgta caagagagag
    38941 tatcctccag tgatcgtaat atcgattagc gccgacagcg ggaaagctat ggatgcctga
    39001 ggctgggcta gaagataagg ccgccagacc gaagcagagc tggccgcgcc cagcgcctcg
    39061 tcaaagtcgg agatcatgcc gcgctttgtg gtaagcgacg ccattccccc tccaagcact
    39121 gtacctaatc cgacatgggc tttcggagaa tccacggcac tgtattcaac caagattccc
    39181 ccgtaattga acgcaagagt agctatcttc ggctctgctc cagcagtagg cttcgaaatg
    39241 gcctcgcctc caccatacca tccgcctatg gaccattttc cgtttccgac accaactgtt
    39301 ccatagccgc cccacgtgag catgccgccg ggaagctggt tgaattcggc atcaaccaga
    39361 gattcattga gatcatcaac cctgaggaaa acccatccga ctgagttccc accgccgccc
    39421 acctgcgcag aacaggcacc cgcagtcaca acgaccaggg ttacggcaac aatcatagat
    39481 acgttcagtt ttcgaagcct ctttgcttgc atagcgtcta cctcacttta cttgcgttcg
    39541 cagagctcta taaggactcc acctgtagct ttcggatgca ggaaagcgat ccgcgctccg
    39601 cctgctccat agcgaggctt ctcgtcgatg agcctgatcc cttcagcccg catcttatcg
    39661 agcgcagcgt cgatgtcgtc cactctcaag gctatgtgct gaattccctg tccccgtttt
    39721 tctatgaact tggcgatcgg accctcagga tcggtcgact caagaagttc tatctccgta
    39781 tcgcctatag gtagaaaagc aactttgacc ttctgctctt ccaccacttc agttccttgg
    39841 agctcaagtc cgaacgtttc ggtatacagt ttgagggcct cttcaagatc tgccaccgca
    39901 attcctatat ggtccaccga tttgaccaaa tcagtcccct cccgcgaatc tatttgacga
    39961 tcatctcata aagctcattg cctgctgaat aagggtcgat ctcccttcgg gcaaccttcc
    40021 acgccagttc ctctgcagca gcgaatgcac cacgatcgtc tgtgagcccg cgccaaatcc
    40081 ttcccgatgc aatctgcatc gtctccgaac gtgcacgcct gtatctccga tcgtccaact
    40141 caccggttcc ggccagataa cgcatatgct catcgcaggc gtcgaaaagt tcgcctaccc
    40201 cttcacccgt ctcagccaat gtctggagga tcggaggatg ccaacagggc ctcgctgcct
    40261 cctcgtccac tgtctcttct ctttcagagc ccgccgctcc gagcgccgaa actccgtgat
    40321 gccccgcgga tccagcgatt ctgctcgttc cgccgcctcc cctagacgca acggaactga
    40381 gctccagagc tatccgaatc tcagctacca ctctttccac acccgggcgg tcttttttgt
    40441 tcacggcgaa cagatcaccg atctccatta cgccggcctt tatgacctga atatcatcgc
    40501 ccaacccagg cacttgcacc agcacgacgg tgtcagcgat cgaggctatc tccacctcgg
    40561 attggcccac acccacagtc tcgatgatta cgtaatcata cccggcagca tccagcacgt
    40621 ccaccgcgcc agacgtagca cgcgatatac cgccaagctg gcccctggtg cccatgcttc
    40681 tgatgaacac tccatcatct aaggcatggc gctgcattcg aagacgatcg cccaaaagcg
    40741 ccccgcctgt gaacgggcta gtagggtcga ccgccactat tcccacagtt tttccgcgtt
    40801 ccctgcacac cgccgccatc ttgtcgacga gtgtgctctt tccggatccg ggcggaccgg
    40861 taatccctat tacgtgcgcc ttccctgtaa acgggtagat ctccgaatac aggccctcga
    40921 atcctggaac acaatcttca atgagggata tgagccggga cacggcccgg atatccccgc
    40981 taagggcccg gctcaccact tccgttcggc ccatgcttac gaccgcctct tcacgttctc
    41041 ttctatgaac ttaaccgttt ccgatgtact tgtgccagga gtgaatatgt ccgctatgcc
    41101 acattctttg agggcaggga tgtcctcatc cgggatgatg cccccgccca cgacgagaat
    41161 atcgtcgaca cccttctcac gcagcaggtc catgaccttc gggaaaagga cattgtgtgc
    41221 acccgaaagt atcgacatgg cgatcacatc gacgtcttcc tgaatagcag ccgccacgat
    41281 ctgctccggc gtctgcctga gccctgtgta tatcacttcc atgcctgcgt cacgaagggc
    41341 acgtgccaca actttggcac ctctgtcatg cccatcgaga cctggctttg cgaccaaaac
    41401 cctaatcttc ctgccagtat cagtacccat gctgtcgccc attctccctt cctgctagta
    41461 ggcccctgag ggcttgtatt ctccgaatac ttcgcgcaaa gtgtcgcata tctctcccaa
    41521 tgtggcgtat tctctgactg cgtcaagaat atacggcacg agattgtcag ttccttcggc
    41581 cgctgccttg agcgcttcaa ggctcttttc cacagcctcg ccattccttg aagctttcac
    41641 atccgccagt tttttgcact gcagatcggc aacggcgggg tctaccttga gaaggccctt
    41701 cggcggctcc tcctctatgt agaattcgtt gacgccgaca acaatccggt ctcccgactc
    41761 tatctccatc tggtaccgat aagcgctctc ttctatctcg ctctggatga accctcgctc
    41821 gattgccata ggcgctccgc ccatctcgtc gatccggcgg atgtaatcgg cggctgcctc
    41881 ctcaatctga gtggtaagag cctctacata gtaagagcct gcaaggggat caatggtgtc
    41941 ggcgacaccg ctctcgtatg cgataatctg ctgcgtgcgc aaggcaatca tcaccgattc
    42001 ctggcttgga agcgctaggg cttcatctcg ggagttggtg tgcagcgact gagtgcctcc
    42061 gagaacagct gcaagcgcct gcaatgccac cctgacaatg ttgttgtcag gctgctgcgc
    42121 cgttagagtg gacccgcccg tctgggtatg gaaccggagc ctcatagact tttccttctt
    42181 ggctccgaac ctttccttca tgatcctcgc ccacagcctt ctcgccgctc tgaacttggc
    42241 aacttcctcg aaaaggtcgt tatgggctgc aaagaagaac gacaacctag gagcgaattc
    42301 gtccacatca agacccgcgt ctatagccgc ctgaacatag gcgattccgt cagcgagcgt
    42361 gaaggcgagt tcctgtactg ctgtggcccc tgcctctctg atatggtagc cacttatgct
    42421 gatggtgttc cagtttggaa cgtttgcaga acaatactcg aatgtgttgg ttatgaggcg
    42481 cattgacggt ttcggtggga aaatgtaggt gccgcgggcg gcatattcct tgaggatgtc
    42541 attctgaata gttccgttaa gcttctccgg cgccacgccc tgtttttccg cgactgcgat
    42601 atacatggcc aaaaggactg cagccggcgc gttgatggtc atggaggtgg aaaccttgtc
    42661 tagcggtatc tcgttgaaaa gaatctccat atccgcaaga gaatctatcg ccaccccaac
    42721 tttcccaact tcccccaatg ccagctcgtg gtcggaatcg tatcctatct gagttgggag
    42781 gtcgaacgca actgaaagcc cggtctgccc ttgttccagc aggtacctgt agcgttcgtt
    42841 cgattcctcc gccgtggcaa agccggcata ttgccgcatt gtccacaagc gcccccgata
    42901 catcgtgggt tgaactccac gcgtgtatgg gtattctccc ggaaatccaa ggtcctcaag
    42961 atagtcgatg ccctcagtat ccaacggtgt gtaaacaggc tggacctcga tgccagaagt
    43021 cccttcgaac ttctcccgtc gttcagggaa cctggcgcag gttttgccgt aggtcgtgct
    43081 cttccaacga tccagctcgc ccttgagatt gctttggttc aattcaacta tccctccaac
    43141 atatacatgc acgagccgcc tcaatcgctg catagctaaa gagacgtacc cttgttttat
    43201 attccacatc atccctcatt cacctgctag actatgacag ggttggagtg tcaagaccag
    43261 agtaagaatg tgaataaagc acagttcaga aatgcaatat ttcgtcagga gatcctgctc
    43321 catcctaccc cgtcgtgttg tcccttttgc attgtgcatc gctttcgctt tgacgcttcc
    43381 atgccagttg caaaccatcc cccaacaatg gcattagctt ctttcgcatg agtataggag
    43441 ttgccaagtt gaagttgccg cgtatgccgg ccccttggct ggcaaagcca gcctgctctt
    43501 ctcgcataat ccataccagt gcgcgcaggt gtttgtcatt gtcgaatgac gcaatagacg
    43561 atcgcgtatc gcctccgcga gcattgaggt tgctgagttg ttggacagtt tgcattgtca
    43621 gaggccagga tcacctaagt aagtctcaac aacttcctga gtgatcctcg agtgcagttt
    43681 aggctttggc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcatgga
    43801 gtttgataga cactaaagaa atccacctgt tgctttcgag tttcaagggc cacagcattt
    43861 cgtgataacg aaagaagagc aacaaaggcg agacgaccgt aaagcaggca tatgcgccag
    43921 tgcggatgtc tatcgccccg actacgctca tcttctggtt cgtgccgggc atcggaatcc
    43981 acacctgctt atcgcgcagc atccacatgg atcttagaat cgggaacata cgcacgccac
    44041 cttcgtcatc ataatcatga agaacatggt tgcctgccgc cagcgagctg aggactgtgc
    44101 ttatagcact cgtcttgtcc cttgcccggg gatcatatcc cggcttggct gagtgtcgag
    44161 ggcagttgtg cctgtgaccc aaacccttca aggaaacata cattgtcgat gcgcagattc
    44221 agaccccgta ttggactgcg gcatagacgc gaaagcgcac cgcagtcaag atggaacatg
    44281 catactcgag tcccgaattg tgcaacatct tgccaggcca ccggcccaaa tagcgctcga
    44341 gaatttccgt cccaaacagg ctattaccta tgcaggaaag tgcattttgt agcagaacta
    44401 ctattctatc aattgcaaga ggggaggctt atccgtgaaa caccaactac gcactcacct
    44461 tgccacagcg ctggcaacgc tgtgcttgtt ggcagtatcc gctggcgctc taggctcatc
    44521 cgttactttg ctagatggaa ccacgtacga tgcagatcta gtaggtttct tcacctttga
    44581 tggagtggac catttcgtct tccgactgac cgacggatat ctcatggcct gtcctcgatc
    44641 tgacgtgatg ctgatacaag tgagcaaatc atccatgggc agcacccagg ttgggcagca
    44701 ggtaccgccc ccagctattg ccatcccttc gacgccgata tcgggtccat tgttcgaact
    44761 ctccggcata tggagcgctg acctcaacgg attcggcggt gagatcagga tcgaacagag
    44821 cggtacaaca ctctcgggta gcgtcttcgg agacaccttg gcaaacggca gagtgtcacc
    44881 tgacggctca gtcgccttcg aacgaatagt cggcagcggc aatcaggtgt atcgtggcac
    44941 ggtctcgcgg gatgcatcag gaaacttgat catagaagga accttcgact gcaatatcac
    45001 aggcggaaaa ggtcttccct ggcatgcagt gttaacgaag ccttccgcgg ggtcaccatc
    45061 ggctcagctt ccgacaacac aacccgcagt cccgccagcg ctcgagccaa gctacttgca
    45121 agcagaacgt gaacccaact ataatatcgc aagcgcaaat ccaatagccg ttaatacaaa
    45181 tgtcacaggc gccatctcca cagatggtga tattgacatg tacaaggtta cggtgaccac
    45241 acatggaact ttccacgtga catgtacaca gcccgcagat ttcttcatgg caatactgaa
    45301 tgaagcggga agtcagatcg ccggagatga tctgggaagc agcgggacaa caatggtgct
    45361 caatgccgac gtttttgaac caggcaacta ctatatactc gtacgcggat actcaggcaa
    45421 catcaagtct gatacccctt acacccttag ggcagagttt cgggaagccc gtgatgcctt
    45481 cgagcccaac gacaacgtta acctggcatc ggcagtcgtc tccggaaggc agttcgattc
    45541 cttcatctat aggcccgggg acatcgatta ctacagcgtc agtgtttctg gacccgccac
    45601 catcaaagtg acgtctacac agccggccga ctactacctc tctatcgtgg acagttccgg
    45661 cacaaaggag ctctacggcg gcgacttagg aagcaaagga actaacatgc ttcttgaagg
    45721 ggacgtatac gacccgggaa cctactacgt agtgatgcgc gggtacaaca aggacatata
    45781 cgaaatgcac actccctaca gcatgaccat aaccgttacg cacggaggta ctgacagatt
    45841 cgagccgaac gacacgcctg ggcaggccgc agtgccgcct cttgggcaaa ccttcgcagc
    45901 gttcatatcc cgcccgggcg acctagatta ctacaaagtg gacatcgctg gaccatctca
    45961 ggttgtggca gaatctgacc aaccagttga cttgcatctc tccctcattg accgcgacgg
    46021 caagacagta ctcctcggcg acgactacgg ccatccaggg aacaagatgg tcctgaccca
    46081 tgacatattc cagcctggaa cgtattacgt attgatgcgg ggatataaca ccaacatata
    46141 cagcatgcac accccttaca acatgaagat cacagtcaag cctgcactgt acgaccccgc
    46201 agaacccaat aattccgcca atgaggccaa gagcattcag ctaggacagc cggtaaccgg
    46261 ggtgatcgca acaggcactg atgaggattg gtggcagttc actgcacccc tcttgggcaa
    46321 ggtgcggata acctgcacac agacggccga cttcagactc agagtcgtgg atgcaacagg
    46381 aaaggagata accaatcagg acctgggcaa caagggaact ctcatggacc tcacgttgca
    46441 gctcacacca ctgaccaggt attacgtagt tgtgcagcca tacagcagca gttgctataa
    46501 catgtttgaa gcttatcaac tcattgtagg ctcagctatc tagtcagctg tcaacagaag
    46561 aagatgagat gaacgatgaa gccgccgcat ccgcggcggc cttccttttt gtttgccgca
    46621 tgctccctgc atggctcccc gacatacccc atcgtgcatc caagctgtcc gggacagagc
    46681 tacctcagca cctcgtaccc agcatcctcc accgcacgaa ccaagtctgc aggctttgcc
    46741 gcgttcctgt cgtatacgac cctggcggac ttagcctcaa gatcaacctc tgccgactcg
    46801 actcctacta cacccataag agcaccctcc acagcggctt tgcaatgccc gcaactcatt
    46861 ccagccacgt tgagcagagc ctcactgtaa tcgcctacca ttccggcagc ggcagcggat
    46921 ttctccgtgg atcccggtcc ggccaccatg ttctccttgc tatctgacat gctcttcctg
    46981 ccatagggca tattatctac ccctttcacc tctgtcctgg aaatccctcg aggcctctct
    47041 tttccgaacc ctctcaatcg caatgaattc gaaactaccg ataccgagct caacgacatc
    47101 gcagccccgg catacactgg cggaagaact ccgaaagccg caagagggat cccaatggta
    47161 ttgtagacaa aggcccagaa aagattctcc ttaattatgg taatagtctt cttagccagc
    47221 ttaatggcat cgaggagctt gcccaaatta cccacaatgg taatatccgc agcttccatg
    47281 gcaacatcag tccctgtact catggcaacc cctacatccg cagtggcaag ggcaggggca
    47341 tcatttatcc catcacccac catggcaacg gcaagcccag cctcctgcag ccgttcaacc
    47401 tcgcgcgcct tgtcctcagg aagaacctca gccaggacgt tgggtttggg tattccaaca
    47461 cgttcggcga tagctgccgc tgtgcgcgcg ttgtcaccgg taatcatata tacgtctatg
    47521 ccgaggtcct gaatcccctt gatggcctcc accgaacccg gccgtacttg atcggcgatt
    47581 ccatagaccg cagcgaccgc tccgtccacc gcctgaacta cggcggtccg cccctcatct
    47641 tgcattctgg caacagaggc ccccacagcg cctgcggcaa tcaaactcgt aaccgggact
    47701 tctgaaccag ccccattcgt ctcctcagcc gtccccgcca ccagcccgct cccgaacctg
    47761 cgctcggctg caagcccttc acggtccacg gatacgccaa gttcatctaa aaatcgaagc
    47821 gttccgacgg caacctgtct tccttccaca gttgcgacga tgccgcgccc cggaaccgtc
    47881 ctaaactcgt cgggcatcgg aatatctatc cctgcctgct cagccgcttc tactatcgcc
    47941 ctgccgagcg gatgctctga agcccgttca gcagaagcgg cgagtcgaag cgcctcttca
    48001 cgcccgtgag gtgcaaagac ttcgacttct tcaagcgacg gcacgcccgc tgtaatcgtc
    48061 ccagtcttgt cgaggactac tgccttcact gagctaagcc tttccagatg ctctccccca
    48121 cgtatcaaga tacccagctc ggcgccacgc ccagtggcta ccatgatagc cgtcggggtc
    48181 gcgagcccga gcgcacaagg acacgcgatg accagcacag ccgtggcgtt caccaatgcc
    48241 tgagacgcgc ctattcctgc agccatccat cctgcaaacg taaccacagc cacaaccaga
    48301 accgcgggga cgaaatagta tgacaccaca tccgccaaac gctgtatcgg cgccttactt
    48361 ccctgcgctt cttccaccat gcggataatc tgagcaagcg cagtctccga gcccacccgg
    48421 gtcgcccgca tcttgagcat cccgtggccg ttgacggtgc ctcctgttat ggcgtcgccc
    48481 gcctgcctgt ccacggggat gctctcgcct gtgagcatcg actcgtctat agcggaaaag
    48541 ccttcgatta tcacgccgtc tacgggcacg cgttcacccg gcctgactat caggatgtcg
    48601 ccgacttcca cttcatccac gggaacctga atttcggtcc cgtcagcctg cagcaccgag
    48661 gcggtcttag gagacaaagc ggcgagtttc cgtatggcct cgctcgtctt gcccttcgcc
    48721 acatgctcca ggttctttcc cagcagcacc aaggtgatga ctactgcagc ggcctcgtaa
    48781 tagacggggc ctgaaacgat gaaggtgtgg accacgctga ggatgtaagc agcgcccgtg
    48841 cctaaggcta caagaacgtc catgttggct ccgccatggg caactgtcct cgcggcgctg
    48901 atatagaagc gggatccagg cacgaacacc actggcgtgg ccaagacgaa gcccacgaag
    48961 gggtcgatga gaaacatcaa cgcctctatc ccgaaaatgt cggctatcat ccccacaacc
    49021 aacggaatgc tgaatgcagc gcctatgtag aacagtcgcc agttgttgat ctcctgttgc
    49081 tttctttcct ctgtgcgcct agcatcctct tcagcatcgc gtgctgcctc cctcgcacca
    49141 tatcccgcag cctctatcgc cttaaagagc cgatccgggc tcgtctgatc aggatcatac
    49201 tgcacccttg cagtctcggc cgccagatta acggcggcat gtgccacccc ggcctcattt
    49261 gccagcgcac gctcgactcg agcggcacat gcggcacacg tcatccccgt tatggcgaag
    49321 tctacctggc gcaggcctct taccttctta tcaggcgtgc cccctacatc cccccgcccg
    49381 ggttgccctt ttttctttcc acgcctctcc ggccccaacc ccgtatccga gcccacgctc
    49441 tccaccttgc cgtgcagtat tatatccatt cgatccgttc tgccgccatt tgccttcaca
    49501 tctatcaccc ccatctggtc tcagttcaca ggcgttttcg ccaccatgct acattcgtca
    49561 ttacaaagag tcaacagatc ctctgcaaga gcctcttccc caaatcagcc cggcatgtgg
    49621 gcattatggc ctgctgccat ttcacatagt gcataaggca ccggaactag ctgcactcat
    49681 cagcaatata ctgccactgg acgactggac gatccaaaat cgaacctgac tgagatgatt
    49741 gaggtgatat cacgtgctgc cttccagaga gttttttaac atggtgcagg aagcgtacaa
    49801 cgatgaagtt gaagccgtag atcgctatac aaggatggca caaatcgctc caaccgaagc
    49861 aattcgccgc ggggtacttc aaataagcag agatgagttg cggcacgctt tcttcttcga
    49921 ttcagtgctc tcgttgatac gtccccctga gaacatatct tgtccagggg ttcgcatgcc
    49981 tgctccggtt gtgcccgagc ccggaccttg gataccgtgt ccgcctgtgg cggagccatg
    50041 gcccgcgccg gccccggagc cgataatgcc ctcaccggaa attccgtacc ctggcatgcc
    50101 aacgccagga tggcctacaa tgccatgccc cggggcgcca atgcccagtc ctattccgtg
    50161 ccctggcatg cctgcacacc cttggtatgg gatgccgacc atgccatgtc cgggtatgcc
    50221 aatgcctagc ccgatgcctt ggccccaaat acctattccg ggaacaccta caatgcccgg
    50281 accaatagcg cctccacccg agactccggg aatgccgtgt ccgccgtact acactcctcc
    50341 acaaccacat gtcccgtcga caccgggtcc gggaccaatt ccaccggtgc cggaattccc
    50401 tgagccgccc ccaatgccgc ctaagggacc atgtctcggt tgtatgccag agaacgcgga
    50461 cgagtagatc tgctatcacc actaaccaat tgggagcacc agctaaatag ttctgcaaga
    50521 gtaacgggct ggggatgatg gcaaaagcga tagccagtgt ccgaaaatca atgcaagagc
    50581 gacgttcaaa cgaaagagca gggacatcga gtagggtggc gccaggtaat cagcctggcg
    50641 tcatccctct cacagaa
//
LOCUS       DAOSYL010000007        49546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_575_length_49546_cov_7.466933, whole genome shotgun sequence.
ACCESSION   DAOSYL010000007 DAOSYL010000000
VERSION     DAOSYL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 49546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_575_length_49546_cov_7.466933"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(634..936)
                     /locus_tag="PLU15_02600"
     CDS             complement(634..936)
                     /locus_tag="PLU15_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HOO29842.1"
                     /translation="MAVRFKKSKKIGKYGRINLSKSGVGASIGVPGFRVSVSPDGKIR
                     RTVSIPGTGIYDTKVVGSAKPEAHKAKQAPKRACQACGRAAGQKDVFCRYCGARLL"
     gene            1537..2133
                     /locus_tag="PLU15_02605"
     CDS             1537..2133
                     /locus_tag="PLU15_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO29843.1"
                     /translation="MYTDLSDEQLIELHLSGDPRGMEAIYDRYFDRIYRLAYSKLGNT
                     PDAEDVTSAVFMKLCRSLGTFRGDSKLSTWIYTVSNNAITDFLRKKRPALSLDADMMM
                     DDGDSVPREIKDDSRGPEELACEDDFARYVMSKLEGLPPHQRTVIELRYFMELSYQEI
                     ADQLGIELGTVKSRLNRAIGALRSMCSSDSEVNANAMR"
     gene            2120..2917
                     /locus_tag="PLU15_02610"
     CDS             2120..2917
                     /locus_tag="PLU15_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29844.1"
                     /translation="MQCDDLGHKNKRIDASTPTGFSHECFEDDATCEKDLAIIEALHS
                     GIAATPEFRASLKQEFMAEARADAARRAESAAGMNKPASEKPRKKSGSKVRPLYARYA
                     AVAAAAAAIVIISIFTTNWLKPARPDVTQLADAPSRRNAASIEERQDELAYGEGNIRG
                     MLAVTSDIKAMGFAPQAGDSAAAVEVSLKSALTDDSLKELSAIANQAGGSVASSGLDA
                     VASMSIPSEVLDHVLSNLDASLGIVDVSRPVEINTAKTCITLYFTAD"
     gene            complement(2904..4868)
                     /locus_tag="PLU15_02615"
     CDS             complement(2904..4868)
                     /locus_tag="PLU15_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOO29845.1"
                     /translation="MARPSRKNGEYERALPPTLLALGMAAVLIVVGAVGGAIWMQLRV
                     PPQLCAVLAEAGGGSGTQFGENDVAALVRYVQKCSSAGFPDVPLTRIQKIIGERSSVA
                     AVEANQEVEAELAREAHEAVLRQNGGEWGSKFHRLRIDRIVDGLSHVAGDDALGYKIA
                     MLDTPQINAFSTADGRIYLTRGLVTTSSDDEIALVIAHEMHHIRSGHWINWWALSAGD
                     GSFDADTENPQEGGAMAEAALALVKMGTKAFSGEASTSYEQEYEADAMAILHAAECGY
                     CVRRIFRILTRLPEMPVTSHPTSSDRIEGASEVLESMGDPSWIAVHNPAEVARLALEN
                     AIAALRGDRPGICAQVVGLDEVASICARTLKTLEGVEDEMRAYNWLGHGWPYADGSRM
                     VVRTALFTPVLCAVDVGVETTLGHSSAGTPGVLGGRLWLVRRDGVWVVVLGQAFSKAR
                     PVSSHWSMVGDELGNAWRWVGRGGWLASGSSAQVVWDGNNGDAGAARGVLEAAARWRD
                     TSIIDFGEHLSTYAKGRIEYVNNANLEDEGGASKDTGAFAGGSSGCDCKQSTASVDGG
                     HDRMNSGAWQGDQEDGSLEAIGWAQFLSGRKAQNWAWSVRCDVHSRTLATVTFSSAIS
                     LDEVPITSNFTRLTMVLEDGEWKVASVSLQ"
     assembly_gap    4910..5009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5013..5504)
                     /locus_tag="PLU15_02620"
     CDS             complement(5013..5504)
                     /locus_tag="PLU15_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO29846.1"
                     /translation="MEAIDAIRGRRSVRKYRPDPVPREIIEEIVDCGRLAPSARNDQP
                     WEFVVVTEQSIREEIAAVATYGKFIADAPVCLVAFSRQTPRFVEDTCAAFQNMLVAAY
                     AHGLGTCWIAGHNTPYEKEIARICKAPGDYRLVALSPLGYPDEECTVHKRELQEVLHW
                     ESF"
     gene            complement(5630..6565)
                     /locus_tag="PLU15_02625"
     CDS             complement(5630..6565)
                     /locus_tag="PLU15_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOO29847.1"
                     /translation="MKNLWIVKDEVTDALNQERPVLAFESTVLTHGLPYPVSRDLALR
                     VESIAREEGCVPATMGIIDGVVHVGMTPEEIDRLAADGAASRAVKASGHDIAFVVASG
                     MSAGVTVSGTLAIAERAGVRVFATGGIGGVHRGASETFDISHDLVSIARSPVVTVCAG
                     AKAILDLPKTMEYLETVGVCVLGYRTDKLPAFYSADSGIPLSWRVESADEVADAFWAR
                     EELGIHGGLLVAAPIPAEKELSSEEIDAAIEDAVALADERGVRGKALTPYLLARLAET
                     TGGRSVEANLGLLENNARVGAEIARAISCKLRERG"
     gene            6917..7492
                     /locus_tag="PLU15_02630"
     CDS             6917..7492
                     /locus_tag="PLU15_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29848.1"
                     /translation="MKTKPFLATVLAGLLLTAGALAYAKTTPPKTADAEAEGLTIEGS
                     GSLQAPAAKLVAEVKILKAVDDLGLSKEQVKSLAKLAHNLDDEVTKGIDAIVKQLEIE
                     KNELLAGPAGDAVHRAESEIADEKAAIGKALSDAQKEAVKILSPAQRSKAMKMIQEIE
                     IPKEARKRLMADGPKMLKALSDLLDQKLATM"
     assembly_gap    7953..8052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8181..9176
                     /locus_tag="PLU15_02635"
     CDS             8181..9176
                     /locus_tag="PLU15_02635"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOO29849.1"
                     /translation="MLYIPNDSNDPRFNLAFEEYVLKNLDMDDPYLLLWRNAPTVVVG
                     RFQNTPDEINADYVEEHGVHVVRRMTGGGAVYHDLGNLNFSFIERKTSEGIDMKLFAR
                     RIAQALQGVGLDVEVSGRNDLTIDGRKFSGNAQYIYHDRVLHHGTILYSSDLDNVQAA
                     LNVKADKIASKGVKSVKSRVTNLIDYMPVPLSIEEFQKSLLLQLFQGAPVRTRVLTNA
                     ELEEINELVAAKYAKWEWNFGRSPAYNVSNRGRFGCGEVAIYLWVDEGIIRSCRIHGD
                     FFTARDIHDLESGLTGIECSKETLSKAYQDLNVANYLEGLGLDEFLKLTTSTKAV"
     gene            complement(9374..10009)
                     /gene="deoC"
                     /locus_tag="PLU15_02640"
     CDS             complement(9374..10009)
                     /gene="deoC"
                     /locus_tag="PLU15_02640"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020278358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOO29850.1"
                     /translation="MIDHTLLKPDATWDQVVTLCEEAALYGFASVCVNPCYCGFAYEL
                     LEGTDVAVCTVIGFPLGAATSMVKAVEVRDAIASGASEVDMVMNIGALRGGELDCVLR
                     DIESVVDAAGGRALVKVIIETGLLTDEEKVRACLIAKRAGADFVKTSTGFGHGGATVE
                     DVALMRRTVGQSMGVKASGGIRDAATARAMIAAGANRIGASAGVKIVTENA"
     gene            10534..11805
                     /locus_tag="PLU15_02645"
     CDS             10534..11805
                     /locus_tag="PLU15_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOO29851.1"
                     /translation="MGQSGYSSCFSGVKASDTRILKSINLLAVLKVLRENSSVSRADI
                     ARITELTPATVSSIVSLLITAGIAKETGYGESTGGRPPVMVEFNPKAFYLAGVDLGVS
                     KIISVVTDLEGNIVSKTRLELDAHQGVDSIMASLFEATHETLEQVRDVRDRIAGLGLS
                     VPGLIDAERGISVFAPNIPGWSDIPIVKVFEDEFAIPCWVENDARAMAIGEAMFGAGR
                     GHDNILCINVGRGIGAGIIMNGEIHRGKQGGAGELGHMTIDPNGPVCPCGNHGCLEAM
                     AAGPAIAAAAIRAVSTGSSTLIRDIVDGKIEAITAEVVSQAAAQGDLLATRLIQEAGR
                     YLGIGIANAVNLLSPDIVIIGGGVARAGDILFDEVRATVQKRAFTAMVNLPPILPSAQ
                     GEEASSVGAAALVFEEMLAVGSVANLMGESS"
     gene            complement(11878..13065)
                     /locus_tag="PLU15_02650"
     CDS             complement(11878..13065)
                     /locus_tag="PLU15_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOO29852.1"
                     /translation="MSEMTECMGDEVVGGEIKVKLARARKYMCERGLDVMILRRFDNF
                     AWITAGGDNRCAGATDIGVASVLITHDDQWVLTSNVEARRFCEEALAHIWAEGIFRMR
                     EHPWYGEAEALGEAAKGFVDGTSLLSYGGRVGADVPLAGAEFVGPELVQLRYPFTKGE
                     ADRYVKVAHGVAEVVERAAREVRRGQTEREIAGRVASDLFAFGYFPTVLLVGADWRVE
                     RYRHPLPTMNLVDKYCMIIVCAHCGGLTVALTRSVHIGEPSPKLRRRHEAAADVCAAM
                     NARTRAGTRWADVMEAMKEEYRAQGFEDEWKRHHQGGPIGYQDREFFAMPQEVGGPDR
                     GVIMPLQAVAWNPSVPGAKSEDSFLVMEDETRVVTVGEGWWPMIEVKIDGVTLQRPDI
                     LVI"
     gene            complement(13062..13883)
                     /locus_tag="PLU15_02655"
     CDS             complement(13062..13883)
                     /locus_tag="PLU15_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29853.1"
                     /translation="MKPKGVSKILVQVLAVLLAIAYLIPVYMTVVTSLKAPEEINLMT
                     AWELPSKPNWESYSTAFKQFAPYLKNSFILAISATLISAVMGSLNGYVLCKYPIPGTN
                     IIMPLIVFGMFIPYQSILVPLFQFMQKTKLYGGLPGLILVHVVYGLPITTLLFRSFYE
                     EIPDELLGAASIDGADFFGIFRYLIMPLSGPPFLVVCIWQFTQIWNEFLFAVTLARPD
                     AQPITVALANLAGGQAVSWNLPMAGSILAALPTVLIYVLLGRYFVRGLLAGALKG"
     gene            complement(13890..15002)
                     /locus_tag="PLU15_02660"
     CDS             complement(13890..15002)
                     /locus_tag="PLU15_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29854.1"
                     /translation="MQSRRDQLTAALIILPSIVLLAVFVYGFIAQTVRVSMTDWGQEA
                     AMSLDPEIHHVGLANYRELFGGFLNMRFRQDITNMFFFTLLFVSLSLLVGLLLATLID
                     QLIWGEAFFRTVFLYPMSLSLVVTGTIWRWLLQPRGGINVLPTLIGLPAGDFLWLSSR
                     EQILIFDWSHIFHYICIAAAVVLAVFAYGHWRRGERKELRAKSIICAVLIGISASGIL
                     TRVKLLDYPEAHGFNRALWGVIVAAVWQMSGYTMALYLAGLRTIPDELREAARVDGAS
                     KFQIYRHVDLPLLKPITVSAIVILGHIALKIFDLIFSMAGPDHVPTSVPAITMFLKTF
                     RGNELAVGSAIGVILLILVSLLIIPYLVNSFKTGKE"
     gene            complement(15090..16316)
                     /locus_tag="PLU15_02665"
     CDS             complement(15090..16316)
                     /locus_tag="PLU15_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO29855.1"
                     /translation="MCAAIVLMFSSAADAAVRGKLEIFSWWAGDEGPALEALISKYEK
                     MYPGATVINSTVTGGSGVNAKAVLKTRMLGGDPPDTFQVHAGQELIGTWVVANRMEDL
                     TWLYEQEGWNKVFPADLLNLLSTDKGIWSVPVNIHRSNVLYFVPGNLAKWGVGVPNTW
                     DEFLEIAPKLKAKGVIPLSVGENWTATHLWESVALSVLGPVKWEQLWNGKLSWRSPEV
                     IRVWELFGEILKYTNTDAPSLSWQQATDMVVKGEAAFNIMGDWASGYMTTTLKLKPGT
                     GFGWAASPGTKGIFMMLSDSFGLPVGCKNKENAIAWLKMLGSRDGQDTFNPLKGSISA
                     RTDSDLTKYDAYLQSAARDWRTNKIVGSLAHGVVANERFMNDFATVMDIFLSTKNAQA
                     AANAAQAVAIQAGIGK"
     gene            complement(16462..17730)
                     /gene="galK"
                     /locus_tag="PLU15_02670"
     CDS             complement(16462..17730)
                     /gene="galK"
                     /locus_tag="PLU15_02670"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOO29856.1"
                     /translation="MAVDSGWLLDQFRQEFGEFADGEVHIARAPGRVNLIGEHTDYSG
                     GFVLPVAIGHDVAIAFSARTDRNVSLYSCDYHQRTQFSLDEILPSADAGWSNYFRGVA
                     WALEGCEYKGKVELKFSGNEGELRLCGLDAVISGDVPEGAGLSSSAAFEVASAISLLA
                     AAGIDVALINATQSRCGLELRRWIALACQHAENSFVGVNCGIMDQMASCMGRADHAVY
                     LDCRNLRHEEVELGLGRAEAALVIYDTGIRRGLVDSEYNHRRQEVELGTRMISGALRR
                     DDIRTLRDIDIALFDEIKGILPSIVARRCEHVIAENERVQGGVEALKRGDLGAFGDLM
                     WQSHESLRDLYGVSCPELDMAVEIAWKTDGVLGARMTGAGFGGCTVSLVHLEALPHLM
                     EALPDAPMRYGAVQPKTFVVDVVDGARIEE"
     gene            complement(17717..18766)
                     /gene="galT"
                     /locus_tag="PLU15_02675"
     CDS             complement(17717..18766)
                     /gene="galT"
                     /locus_tag="PLU15_02675"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOO29857.1"
                     /translation="MRWNPVLKQWVITATHRQERTYKPPEDFCPLCPTRDTSFPTEVP
                     DYSYEIVVFENKFPSFRHVPPEPAVEGTGVLRVQPAQGICEVVLYTDEHHTTFADLDI
                     DHITNLIAVWTDRYSELGSIDYVDYVLIFENKGDAVGVTLHHPHGQIYAFPYIPPIPA
                     KELESAEEYRDATGRCLFCDILEQELDDGRRVIAGNSHFAAVVPFYARYPYEVHVTSK
                     SHRSCLPDLDGDERRSLAEILKSVTRGYDHLFGFSLPYMMIMHQAPTAKGDWSHCHFH
                     IEFYPLHRTATKIKYLAGCESGGGTFINDTLPEETAEELRTAIERATSADAALACSCE
                     TTEGGVRGGRAGGGR"
     gene            complement(18699..18905)
                     /locus_tag="PLU15_02680"
     CDS             complement(18699..18905)
                     /locus_tag="PLU15_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29858.1"
                     /translation="MIKKKIRKDDGRYLIYYSFPLNNGKGGDEYLAIRADDEGGVTDS
                     VGDAVEPCAQAMGHNCNPQAGKDV"
     gene            complement(19674..20834)
                     /locus_tag="PLU15_02685"
     CDS             complement(19674..20834)
                     /locus_tag="PLU15_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO29859.1"
                     /translation="MGQMNCTTMDIPDDFVARAKRDLADVWEGRSIEPVILLAPENPA
                     LPDYSVAERMEDPEKDLEYQLASAHWDLACLRAGFCNIPSIWPYMGVGVIPSAYGCEI
                     RITPGEDPWTIPITDDPDDVYRLKKPDLARSGLAAKVLDRIGYFSEMTRGTVPIRLTD
                     VQSPMDTATQILNYEELLIAMYTAPDAVHFLLEMVTDSLIEFVRMQEARAVNFFGHTH
                     VNLWRPRGIYVSDDVTAVVSPQHYREFMKAPNERLSRAFGGISIHCCRGYEQNLMEVA
                     NLEGFMSFDADSHYNSIDSIGSALTAVDGCPNWGGAGEEKPLREDERRSRGIWHAWAE
                     NADEAIYRVRAARRWGFGMILMLKCGGVAEGVEAAREIHRATEGGVWLTDED"
     gene            complement(20984..21838)
                     /locus_tag="PLU15_02690"
     CDS             complement(20984..21838)
                     /locus_tag="PLU15_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO29860.1"
                     /translation="MAGLVETLQDMGLSLNEAKAYQALLRYGAANGYEVSKRSGITRS
                     VVYGVLDRLLEKGFVLQVDSDPVMYAPLPPSQLLKEYRELYDRNLDNLERSLKKMESG
                     VSEESYILGVAGYEDTIRKARELIRGTKHEVVVSAWGSDCVPLKEELEAAEGAGRTVI
                     IFCHTKVPFSVGTIYEYGLAEDIIKEKWPNRRIIVATDCRNVLVGDIRHDENLGIVTT
                     NPMIVQMAVEHVILDILHLAQLKAGIGDLAEQIQSGADYLRVIEDQHAKLDLGGAVLP
                     TPVPLPSD"
     gene            21974..22114
                     /locus_tag="PLU15_02695"
     CDS             21974..22114
                     /locus_tag="PLU15_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29861.1"
                     /translation="MWRTRFASIFAYNHALVFRIPAILLSFKEIAREPQNVELRNGGL
                     SK"
     gene            22111..23388
                     /locus_tag="PLU15_02700"
     CDS             22111..23388
                     /locus_tag="PLU15_02700"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOO29862.1"
                     /translation="MTCLDDILADGALTRVLRSFKEATGIAARIVKATGAPAIASRTW
                     EDCAFCRLVRSSEEGRNRCSMSYAYAARQAAYLGDLYVFQCHAGLVCWAAPLVSDGTL
                     LGAVICGQVIMWEPDEYLIRDSVERLRDLDIPLPQLEAAITQLEQVTTRRVQAAAELL
                     FAVSTYIVRSEDLALEQRNEIYRQQRLLGEAIQERKRIEEQFSIRGLYSPQQERELLS
                     AVRSGQRTEAKRMLNEILADIFLSQPDRPDIIKARLLELAVFLARAAVEAGADPERIL
                     GLNYQSIGDLSRCETFEEVCFWIVRVMDEFMDAVQDTPDLPSGQVQEAIRFMQSNLAS
                     KLSLADIASSVHISPSHLSHLFKEETGMSVMDCLTALRISEAKHLLAMPQLTVAQVSA
                     AVGFQDPAHFSRSFKRMEGISPTAYRQSMISAG"
     gene            23498..24115
                     /locus_tag="PLU15_02705"
     CDS             23498..24115
                     /locus_tag="PLU15_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOO29863.1"
                     /translation="MREALESGNAPKVKALAEQALAEGRSATEILNEGLIAAMSVVGV
                     KFKNNEIYVPEVMVAARAMTAGLNVIRPVLVETGAPSEGKVVIGTVKGDLHDIGKNLV
                     KMMFEGAGFEVVDLGVDVSADAFVDAVRNERPDILGLSALLTTTMPAMKTTIDALEEA
                     GIRDQVKVIVGGAPVTDHYAREIKADGYAADASSAVDLGRRLISA"
     gene            24128..25105
                     /locus_tag="PLU15_02710"
     CDS             24128..25105
                     /locus_tag="PLU15_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO29864.1"
                     /translation="MKLIELAMSGKRRLVAPLLGYPGAKLTGSSLKQNEFNPALHAKT
                     MDRIAARFAPDAVFFMMDLSLEAGALGLPVRYPLYESPSVEEHPIREVSDLDALRVLD
                     PLDDARLAGYIKTMELMKRSISVPCAAYVAGPFTLAGLLMGASDIAMATITDPELVHE
                     IVRFAQDICIRYSRALTEAGADMIVVLEPTATFLSPRSFATFCGTYVREVFEAPGMAD
                     MMVLHICGDTTHILREMCATGAHGLSLDAPVNMRAAAGVVDPDVVLIGNVDPVGVMLY
                     RDAAGVAQAVRELLDTMSGVENFILSTGCDLPYETPHENIDAFMSEGRR"
     gene            25109..27079
                     /locus_tag="PLU15_02715"
     CDS             25109..27079
                     /locus_tag="PLU15_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOO29865.1"
                     /translation="MASIILSTEKMKIPANPGDTISSALGRAGVHVYAPCAGRGVCGK
                     CKVVVEGGLGISAPTEIELAHLSANEIASGARLACQATILGDADITVLSRVAPGNQNA
                     SILTDSKWLDNQVASANARVEAKTVSVDPTPSARSDWERISLAFGLPKYCQIADGILS
                     ECPEPASIPTLKLAQSVGEAMAEATSPAQSAGTSSSSIVPCADNGIALTGVFIDGKLA
                     DVFAATAKPTRRGESGGRHPLGIALDIGTTTLVGYLVNLATNTVIGVKGTVNPQTAHG
                     ADLISRIAYSQSKEGLETLRLEVIEAVNELANGLLADADASPDDVYLVGAVGNTCMHH
                     LFCGISPSRLARLPYAPAVLESAPCSPAEVGLACANPRGKFFFLPNIAGFVGSDALAV
                     ALVSGMSKSSAPTLAIDIGTNGEIMLAANGRILACSTAAGPAFEGVNISCGMIAAPGA
                     IDGVRMGKDDIECQVIGDASPRGICGSGLISLIAVLREAGMISETGAFDPDRVDPGSP
                     LRSRLIKDQAGLQFVLAKSGSQGPDVVLTQRDVRELQLAKAAIRAGVEILLAEIGIEA
                     EELSQIVLAGAFGTYLDTEAATAIGLLPDAGDARLVSLGNAAGQGVISALVSAKAYRE
                     AQNLAKTIEHVELGASPLFMEAFTDSMLLSRG"
     gene            27148..27912
                     /locus_tag="PLU15_02720"
     CDS             27148..27912
                     /locus_tag="PLU15_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29866.1"
                     /translation="MDDFLLKQTVLTLKPEPVPAEDVLRSLGMSDVIVKLEADNCLPP
                     DISNDVALLATLAAIDEANRAVIDGLVVPRVVATTLPIASIADKGIFLDSIAENSSAF
                     IPCSGLLFEGASHVMLAIATIGPLLERQVKAEFDTGDPLAGIVLDAMGTISLQRLASR
                     IRQTCAQRADECGMETGPRIAPGCEAMPLDAQSALFSLLDADAIGVTLSDSLLMTPLK
                     STSLVLPLGPSLPDHLTRFNMCDTCPHKNRCANIIL"
     gene            complement(28067..28810)
                     /locus_tag="PLU15_02725"
     CDS             complement(28067..28810)
                     /locus_tag="PLU15_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO29867.1"
                     /translation="MKVCDCHCHVGEFYDQINKKHVSYSPAELVKCMHEADVDLAIVS
                     NISAVDAPMQANFELAEWVEEYRELVPIAWTTPEVTKVGEVRQLLALGFRGLKFHPTA
                     GGYRADSNEVDRYLRICAQEGKPALFHCAADESSAPERYMALAERNPDVWIILAHMNL
                     FGPAEAAIRVAEAYPNICLDTSWARPEDVKDAILRIGAERILWGTDAPLGGECHYRRD
                     RVRAALESKLDEGELKSVMWGNAARLFSL"
     assembly_gap    29080..29179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29443..30648
                     /locus_tag="PLU15_02730"
     CDS             29443..30648
                     /locus_tag="PLU15_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29868.1"
                     /translation="MKVKRLFLSVVWSALILSLAIIPSMAADPLVVWCEPQKMGTIEP
                     LADQWSKKTGIPVKVEPVGVLETANKVQLAGPMGKGPDVFCSVSGSLGMLVVTGTVAP
                     VNPKTFDLSNFMKVSVDAATFNGKLYGVPYDISTVAMIYNKKIWPEPPKTFNEMIDRS
                     RELRKRGKFGILWPMESFYFSYAIMAGYGGYIFAPTESGWDTKDIGLANPGSVKAIKL
                     LKTLRDEGIIPVGTDHVVAPARFSEGNAAAIIDGSWALPGIKQAGIDYGVAPLPKLDN
                     DKYPAPFVSLKWWHISSYSQRKTDAANLISYLTTKEAMYKSFKAAEGIPPRLDVLQMP
                     DVKSMPEVAGYGAQANYGSILPDIPEVNPVWAPMDSALEIAIRGDKSVEAALSDAVKI
                     IRQNIAELK"
     gene            30806..32488
                     /locus_tag="PLU15_02735"
     CDS             30806..32488
                     /locus_tag="PLU15_02735"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOO29869.1"
                     /translation="MEKILMMTGGGFHPFHLCSSIAKDFLEQTGIFTVEITEDRDKFL
                     DLSNYSAVIVYTQGGELTAKQENGLLTYVRSGGGIVGIHSAADSWTNNKGYMEMIGAQ
                     FREHGPMTDITVTYSKEADAVIPRADKSFVIHDEFYMLKPHAEEALKPFMYGSWHQAQ
                     EILGYVRDYGKGKVLYTALGHDERAFNVPQFQELIIKGLMYVIGKISDETIRFGIVGY
                     GPLYGMGKYHADSINNTTGLITTAICDKDPARLRAAKNELGDIPTFTDASEMAQSGLV
                     DAAVVAVPPNAHAEVVLALIESGLHVVSEKPFAITTAEVDQMIEAAQCRDVVLSVFHN
                     RHWDSDIVTLRQIMDLGLIGEVYSVECNMVSYQMPYPGWRSQKEVSGGLLYDMGVHQF
                     EKAFQIAAYNWGQDCRSRKAFVYGNRLKKAWHHVNVEDYWRAYVKFESGLEMQVVQSN
                     LCAAPKPLWVVLGTRGAAVIESWDWEKPAIVNTYVNNRLTRVEYPIIPPCSIPDYFDR
                     LAEKYYRNLADHVLMDLPLIITPEWARNPVAAIELSEKASLENRGLEAEFIF"
     gene            32702..33799
                     /locus_tag="PLU15_02740"
     CDS             32702..33799
                     /locus_tag="PLU15_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO29870.1"
                     /translation="MFHDISYYKPDLHDCINRVRDFFSLEPGAPQALIYTFPSVLTLR
                     GPALNQYDFEHDMQSYLDDYLEYQAQLMEIRLGIEDDWILSVVPYMGIGEFSAFVAGE
                     IEFGEDTSWAAPVINEKSDLETLCLDPNNKWFRKLNEATRYMVRKIAPYRIPLGRGYY
                     SPLDLAWALRGEAIYMDFYEDPQFVHNLMEFALSATEWFARAQAAEIFAPDVVHELSA
                     WHCGPNRVAVSEDISSLISPSHYAQYGAPYTQRLFDSFGIGEVHCHSAGPHVVPEFLK
                     LKRVRQIQIVADPNTRRPVEILADLMDKNPELFGKSPDVPVIAVDASPQEAVDYYDLS
                     RQTRIVFSVVTENAEEARHAVDIFRAKQKQC"
     gene            33985..35280
                     /locus_tag="PLU15_02745"
     CDS             33985..35280
                     /locus_tag="PLU15_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29871.1"
                     /translation="MATANPHPQVAGILSALLAGAGQAYNGQRTKGIAYMAVEAVFLV
                     LLFTVLRDPLYGLVTLNPVGRFVDSRHILLAGIIAAFVVAIYAWFHIANIVDAVRTAS
                     SIRKRHALPDDMQRIHPEYRTPAGTIAPYLYIAPSLLVAFFVIVIPLAFGIALAFTNY
                     NLYHCPPAGRFDWIGLENFRRLLKPGSLWKDQLDMVLVWNIVYALLGTVLAFLCGLAL
                     SLMLENRHIRFKGAFRTILMLPWAVPATVSIMVFFGLFNTTFGPINEILKNIGLKAVP
                     WFQDRTWARVSVVLTHVWISTPFNLSVISAALQSIPEEIYEAATVDGASPWQSFSKIT
                     FPLLMSVVAPILVLTLAGNFNNFGIIYLLTGGGPAVAGSKGAGATDILMTWVYDLGFQ
                     QLQWSIASALAVLVFIFVVVFSLINFRLSGVLRQLREEE"
     gene            35296..36162
                     /locus_tag="PLU15_02750"
     CDS             35296..36162
                     /locus_tag="PLU15_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29872.1"
                     /translation="MSANTATGKNARKAKRTVGTVLIYAGLIIACIIVLYPLLWVLSC
                     SFKPTAGLVGSSLIPSNPTLANYRKIFTDPNVNFSRWFFNTLKISVVSALLTLVLTTP
                     AAYAFSRMRFPGRRQTLIGFMICQMFPGFMAIVALYTLLGWAGLIDTHLGLIILYAGG
                     AIPFSIWLLKGYFDSVPVEIEESAMIDGATKFQAFTRILLPLAKPILYVVGLINFLGP
                     YAEYLLAQVVITSGSKWTLAMGMRSLTVSQFATDWPIFSAVSVLTAVPIVIVFLSAEK
                     YIVSGLTRGAIK"
     gene            36280..37485
                     /locus_tag="PLU15_02755"
     CDS             36280..37485
                     /locus_tag="PLU15_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29873.1"
                     /translation="MKVKRGFLAVVCGALILSLATIPSMAADPLVVWCEPQKMGTIEP
                     LAAQWSKQTGIPVKVEPVGVLETANKVQLAGPMGKGPDVFCCLSGQLGMLVVTGTVAP
                     VDPKVFDLSNFMKVGVDAATFDGKLYGVPYDISSVAMIYNKKIWPEAPKTFDEMIARS
                     RDLQKKGMFGILWPLENFYFTYAIMAGYGGYVFAPTKSGWNTKDIGLANPGSVKAIKL
                     LKTLRDEGLIPVGTDHVVAPAKFSEGKAAAIIDGSWALPGIKQAGIDYGVAPIPRLDN
                     GTYPAPFVSLKWWHVSSYSQHKAEAAKLIAYLTTKDAMYKSFKAAEGIPPRLDVLEMP
                     DVKVMPEVAGYGAQANFGSILPDIPEMNPVWVPMNNAIEIALRGDKSVEAALADAVKI
                     IEQNIAELK"
     gene            37647..38744
                     /locus_tag="PLU15_02760"
     CDS             37647..38744
                     /locus_tag="PLU15_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO29874.1"
                     /translation="MTHQFAVPFSDPKPDFEGFRQVLEGKRDANRVHYAELFADREIV
                     EAILADYMGLTPVPSPEDDFEEYWRQQIEYWYKMGYDYIRVAGGLEIPRAKHRIAEDT
                     AALSRGERGWIEQGIGEIASWADFEEYPWQTVEDMDFSAYEFVAKNLPEGMKMLVCPS
                     SGVFEISSEYMLGFEGMSMMLYEDPELVEAVFLRVGQMLFDFYRNAVTIDNVAGIFQG
                     DDLGFKTSTFLSPTHLRKLVFPWHKRYAELAHERGQVYWLHSCGNLQNVIDDLIDDVG
                     IDAIHSFQDEIMPVADFKDEYGHRVGVLGGIDIDKLCRLDEADLRSYVRTTIDRCMPR
                     GWALGSGNSIANYVPVRSFLIMMDEGAKWRG"
     gene            38942..40117
                     /locus_tag="PLU15_02765"
     CDS             38942..40117
                     /locus_tag="PLU15_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOO29875.1"
                     /translation="MNTGKKVVLSLQHTIAMFGATVLVPYLTGINPSTALLTAGLGTL
                     LFHLITGGQIPIFLGSSFAFIVVIQTVAEKFGMAYAMGGIVVAGGVYVLVAILVKLIG
                     YDFFKFLFPPVVTGSVITVIGLTLAPTAIQMASSNWPIALAVFAIAAISTLAFKGFMQ
                     MLPILIAVVSGYVISLIAGIVDFAPIADAAWFGVPELTFPKFSWEAIAIIAPVAIVTL
                     IEHWGDINANGAVVGKDFVKEPGLWRTILGDGLATMLAGALGGPANTTYSENTATLSL
                     TGVYDPQILRGAAVFAIIIAFCGKLGGIIQSIPEPVMGGISILLFGMIASVGISTMFS
                     GADMNSTRNRIIAAAVLVFGISKVVVPVGPFEFEGMSLATMTGAILNLVLPQDRPDE"
     gene            40696..41958
                     /locus_tag="PLU15_02770"
     CDS             40696..41958
                     /locus_tag="PLU15_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO29876.1"
                     /translation="MKRVIALILALAALVAVSSPAFGAVTLEVVWMGWPKNLVDKQLE
                     DFRRANPDISLDIQLVPFNQLFQTLEVRLASSRPPDVFIVDGPMTASYAERGYLMPLD
                     SHLSSKELSAWFPASVEACRYRGKLYTLPYATSSVGLYFNKDIFREHNVKLPPEDPGH
                     RLTWEQVVDIARRLTIDTDGDGQPDIWGLIIEQCDRPYQIVPLAESKGGRPLSPDGTI
                     STGYVNSAAFVEAASFYWRLFNDWKVSPKGILQAPIAREYFGNGKAAMMLGAEWNIAQ
                     LRNFPNYEFGLSPHPYFAAGREVTPTGGWHVGISSKTSKKDAALRFAKYITGHDAVAT
                     WAKLFGNAPARRDVYHSLNEMFSDLMWQVFIYEMENTASPRPVTAGYLEYESALKEAF
                     SAIQYGADPAATMNNVAKQIDRELRKYR"
     gene            41977..42846
                     /locus_tag="PLU15_02775"
     CDS             41977..42846
                     /locus_tag="PLU15_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29877.1"
                     /translation="MTYRDSKAAFGFLAPALVFLCFFRLWPAAKSVAESLRGWSSAGA
                     KTFVGLANYRYLFREDPAFWASVRVTLKYAAIASPITIAASLGLAFLLRGGGKRSSFL
                     RTLCFLPSAISLSVVAIVWGLILDPYYGLANSILGKLGIAPQPFLSSPDQALACLIFI
                     TVWRNAGYWMMFFLAGMEAVPAEVYEASALDGASCMQQAWRITLPLLARTMSFVLVAN
                     TTSNFVTFAPVFILTQGGPIGSTNVLMYESYKSAFVYLDTGRAMAISSLTLLGVLALS
                     AMQLKLTRAQFEH"
     gene            42987..43922
                     /locus_tag="PLU15_02780"
     CDS             42987..43922
                     /locus_tag="PLU15_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29878.1"
                     /translation="MRSRKSDKLAGPRAGRTISFRRNRDGVDFVPQLSTGKALASISK
                     EILMLAVAIIYLVPLWWVVASSLRPAGSLYQYATPISWRTFFPDELGIANFVNIFAEL
                     DFGQAMSNTLLVSTITVALGLLVNSMAGFALAKFDFPCKRLVFIVVLITFMAPFDTIV
                     IPLHQVISDLGMINTRAALILPAVGNGLVIFMFKQFYQEIPSELLDAARADGASWPRI
                     FWQVVMPISMPAVATAAVMMFLFQWNSLFWPLIAAFSPKYRTVQVGVATQISVDQTHW
                     ANLFASAVMGSLPPIILFLSLQKRYLKGISSLGND"
     gene            complement(44283..45059)
                     /locus_tag="PLU15_02785"
     CDS             complement(44283..45059)
                     /locus_tag="PLU15_02785"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOO29879.1"
                     /translation="MKDDFALWQEYGKTNSSAVREQLIVKYVHLVKHIVSRMPQAAIP
                     GVDYDDLIGYGVLGLIDAIERFDMSRGVKFETYAMTRIRGSIIDNLRRMDWAPRSVRH
                     KARQIECAMADAESVTGRPATDEEIADRLKVDIDTLHRWTWEISMASYSSLDELIAVD
                     DEHNVATLLDFVSDDGSPDPEMILEKDELYDALADAIDHLPEREGLVIKLYYFEEMTI
                     KEIARVLGVSESRVSQLHTKAMMRIRVEIMESRGCQCQPR"
     gene            complement(45066..45737)
                     /locus_tag="PLU15_02790"
     CDS             complement(45066..45737)
                     /locus_tag="PLU15_02790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO29880.1"
                     /translation="MDFPPLKPNARIEVRIKGYDESPKFATKVEGVSKSGYWIYAPYT
                     DDWRSMIRKGDEIEVAYPYRGAIYHYHSEVIGESGRRVKLLEISEPLFVRREQRREFV
                     RVPILLDVQYAPDCDDNGEVQWESGVAVDLSGGGTCIFLADPPSWLKCGETLNLILPI
                     GPDGGPFKVPAEIVRIEESDRPEQPGVSVGLSFIGITDREETAIVRFVFDRQRELIRK
                     AIDPE"
     gene            complement(45748..46659)
                     /locus_tag="PLU15_02795"
     CDS             complement(45748..46659)
                     /locus_tag="PLU15_02795"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOO29881.1"
                     /translation="MSDQAEELREAASRVQGKESRRKVARGESDDTTRVVAVTSGKGG
                     VGKTSFVVNLAMLLADFGMRVTILDADLGLANVDVLLGLAARHNLHHVIKGTLSLEDI
                     IVTGPRDVQIIPGASGLRDIADMAPEDRKRLIESLSRAVRGRDLLIIDTAAGMSRTVL
                     DFVLSAQEAIVITAPEPTAMTDAYAMIKVISRENPNAIVRIVVNMCHSRKEAENVVEQ
                     LSLLSDRFLDFTFDALGYIPVDPSVSQAIRQRQPFVLAYPYSPASAAMESIARRLRGM
                     PQVNEGQAGVGQFIKRLLGVTAPDYLL"
     gene            complement(46707..47954)
                     /gene="flhF"
                     /locus_tag="PLU15_02800"
     CDS             complement(46707..47954)
                     /gene="flhF"
                     /locus_tag="PLU15_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOO29882.1"
                     /translation="MKEALDKVRADLGPEAVILDARQCRARGFAKVLSFFGMNRKPAF
                     EVLAAIDGDEAVWAPKFSVALERAEQNEAEIRSVSALSQSDGEPLPEGDARRREPDKG
                     VSEESGQPGRGDFTPDRQGIGFVTESQGVDATLNVIARESAVRARVSSLPPEMAAVYQ
                     FLVEEDVSEKTAFRLANAVASECGNIPAESDEILRWAFDEVAKMVDVSGPIEIVRGRR
                     KVVAFVGPTGVGKTTTIAKIAAGAALVEKAKVALITADTYRIAAIEQIKTYAEIIGVP
                     WEVVYSPRDMEMALVKRSDAELVLIDTAGRSPNSPTRMYELKSMMDAALPEEIHLVLS
                     ATTRRQDLDNLVDRFAQVGFDKVILTKLDESSTLGAIFNVYARSGRPFSYFADGQSVP
                     EDIHPATCSEIAEMISSHWRKTV"
     gene            complement(47965..>49546)
                     /locus_tag="PLU15_02805"
     CDS             complement(47965..>49546)
                     /locus_tag="PLU15_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOO29883.1"
                     /translation="EEEARMRRRKIQQEADFYGAMDGASKFVRGDAIAGIIIILVNIA
                     GGIIVGMVRFGLSGGEAFQRYALLTVGGGLASQIPSLLISTAMGIVVTRTASESDLGQ
                     EFTSQMFRQPRALAITSILLLILALMPGLPKIPFLLLAFGTGFLAYRVTKEIHEEEAA
                     ARMRDVVPPKPKTDAEDVMALLRVEPMELNFGYALIPLVDVDQGGDLKDRVAAVRRQI
                     ALELGIVVPSVRIRDSIQARPNEYVVKIKGVEVARGEVLSDCYMAMNPGTATEEIEGT
                     HTREPAFGLPAIWIPAARKAEAEMLGYTVVDPASVIATHLSEIIKANAPELLSRQDVQ
                     NLIDNVKKTAPAVVDELIPAQMTVGEVQRVLQNLVRERISIRDLVTILEALGDAAGTY
                     RDIDTLTEYVRSRMARSICSQYAQPEGLYVVTLDPSLEQLLFDSLDVTEHGTYLAPDP
                     KVYRALISSLVAESERMASGGLQPIVLTSSGLRPHFRRLIERAVPQTVVLSYDEIAPE
                     VKVNAVGMVSLGNAGEEV"
BASE COUNT        11515 a        14532 c        12285 g        10914 t
ORIGIN      
        1 gtcgcttttc gggcgcgggt gtcaaagttt tagactggtc tgtaaaacct gctcgccccc
       61 gcggccagct ccacgccgca cggcgcttcc ctaggcttct accgaaggcc aaacttacaa
      121 gtaaagacct tagcagtccc ccggctaagt cgcagacacc ggcagtacaa actcgagggg
      181 cgtgcagaga tttgttgcgg tctaattagg aattagacat ccctgttcat tttcgcacat
      241 ttcaaggtcg caactgtcca atttccagtc gcttttcccg accacgaaaa tggcactgcc
      301 tcctaaagtc cgcctctacc gcaccacgtg tgcgcaaagc cctaaagata gatcctcagg
      361 catggtccgg aaaagcgaac ccaaacccaa aaaatgcaca cgtcatccag tatccgcatg
      421 cactcgaagg tgtatgcaga cgaccgcaca ggttccttcc agcatttcca agcagctcag
      481 gggaaatgcc ttgggccaat tgagcggcag aagcctgaaa tacgaggaac gcaaacccga
      541 aagtcgcaca agaacttggc aagactcgca ccttgccctg acaaactcca ccatatgcct
      601 tcaaaggcaa ccgccctcgc aggcaacaca gccctacaac agcctcgctc cacagtaccg
      661 acagaaaaca tccttctgtc cggcagctcg cccacacgcc tggcacgccc gtttgggcgc
      721 ctgctttgcc ttatgcgcct cgggttttgc tgagcccaca accttcgtat catagattcc
      781 cgtgcctgga atgctcacag tgcgcctgat cttcccatcc gggctgacac tgacccgaaa
      841 acccgggaca ccaatagaag cgccgacccc cgatttgctc aggtttatcc tgccgtattt
      901 gccgattttc ttcgactttt tgaacctgac cgccactaca ctccctccta gccaaaaatc
      961 ccggctatta gcaggcgcct cagcttgcgc caacaccgct tcgcttcaaa atcccgcccg
     1021 cctttcgcct tgccttcccc tctataatta tagaatgaac gcaaaagccc ttcagcaatc
     1081 ctgcacaaac ctctcaccgt gtttcaacct aaaggagaat aagcccgatg gccaacacta
     1141 ggacgcagcc aacctaatga gcattgctac cggaaacaag ttctacactc cgtacctgct
     1201 ttacaatgtt caggccgaaa cctctgtctt catggcatgt cccctttgcc aaaaccccaa
     1261 agatcagacg ctcggatttc cctccgcccc gcatttggaa cttcctcttt caagccgcgt
     1321 catatcggtt gagaaggagt atgcccggag cgaaaggcct aggcctgaac cgtcgcctat
     1381 ctcccccgga aacttgccct tcctacctgc cagcaatcat ttggctgagg caggcaggaa
     1441 caccagggcg gatgccgtag tactattctg ttggggattg cggattttat cgccaccccc
     1501 agcaggaatc gacaggcatc ctaaggggtg agccaggtgt atacggatct ctctgatgag
     1561 cagcttatag agcttcacct ttcgggcgac ccgagaggaa tggaagcgat ctacgataga
     1621 tacttcgatc gcatatacag gctggcctac tcgaagctgg gaaatacccc cgacgccgaa
     1681 gacgtaacat ccgccgtttt tatgaaacta tgcagatccc tcggcacgtt caggggtgac
     1741 tcaaagctct cgacgtggat atacactgta tcgaacaatg cgataaccga ctttctgaga
     1801 aaaaagcgtc cggctctgtc gctggacgca gacatgatga tggatgacgg tgactctgtg
     1861 ccgcgcgaga tcaaagacga ctcccgcgga cccgaagaac tggcgtgcga ggatgatttc
     1921 gcccgctatg taatgagcaa acttgagggg cttccgcccc atcagcgtac agtcattgag
     1981 ctcaggtatt tcatggaact gagctatcaa gagatcgcag accaactcgg gatcgaactg
     2041 ggaacagtga agtccaggtt gaatagggca attggcgccc tgcgatccat gtgttcctct
     2101 gacagtgagg tgaatgcaaa tgcaatgcga tgatctgggc cataagaaca agagaataga
     2161 tgcatccact ccgacgggct tttcgcacga gtgcttcgaa gacgacgcca catgcgaaaa
     2221 ggacctcgct atcatcgagg cccttcactc cggtatagcg gcaacccccg aatttcgggc
     2281 cagcctcaaa caggagttca tggcagaggc cagagccgat gcagccaggc gtgcagagag
     2341 cgcagccgga atgaacaagc ctgcatcgga aaagccccgt aaaaagagcg gcagcaaggt
     2401 caggccgctt tatgcgagat atgcagctgt agccgcagcc gcagccgcca tcgtaatcat
     2461 atcgattttc accacgaact ggctgaagcc cgcgcgccct gacgtgacac agcttgcaga
     2521 tgccccgtcc cggagaaacg ctgcctccat agaagaaagg caagatgaat tggcatacgg
     2581 cgaagggaac attcgaggca tgctagccgt gacgtccgat atcaaggcga tgggctttgc
     2641 gcctcaggca ggcgacagcg cagcggcggt agaagtcagc ctcaaatcag cgttgacaga
     2701 tgactcgctc aaagagcttt cagctattgc caaccaagca ggcgggtcag tggcttcatc
     2761 cggtctagat gcagttgcat ccatgagcat cccctctgag gtgctcgacc atgtcctgtc
     2821 gaacctcgat gcaagcctcg gaatagtgga cgtgtcaagg cctgttgaga tcaacaccgc
     2881 aaaaacatgt atcaccctgt atttcactgc agactgacag acgccacctt ccactcgccg
     2941 tcctctagaa ccatcgtgag cctcgtgaag ttgctcgtta tgggcacttc atcgaggctt
     3001 attgccgacg aaaaggtgac cgtcgccaaa gtacgactgt gcacatcaca cctgacgctc
     3061 cacgcccagt tttgggcctt ccgtccgctt aaaaactgcg cccagcctat cgcttcaagg
     3121 ctcccatctt cctgatcacc ttgccatgca ccagagttca ttcggtcatg accgccatcg
     3181 accgatgccg tactctgttt gcaatcacag ccagaacttc ccccggcaaa agccccagta
     3241 tccttgctcg ctccaccttc atcctccaaa ttggcgttat tcacatactc tatcctgccc
     3301 tttgcataag tcgaaaggtg ctccccgaag tcgataatgc tcgtatcccg ccagcgcgca
     3361 gcggcctcca acacgccccg ggccgcgccc gcatctccat tattgccatc ccaaaccacc
     3421 tgcgcagaac tgcccgaagc taaccaccct cctctaccta cccaacgcca ggcgttgccc
     3481 agttcatccc cgaccatcga ccagtgggaa gagaccggac gcgccttcga gaatgcctgg
     3541 cccaatacga ccacccatac tccgtcccta cgaaccagcc acagtctgcc tcccagtact
     3601 ccgggggttc ccgcagaaga atggcccaga gtcgtctcaa ccccgacatc gaccgcacac
     3661 aacaccggcg tgaaaagggc ggtgcgaacc accatcctgc tgccgtcggc atacggccac
     3721 ccatgaccca gccagttgta ggccctcatc tcatcctcca cgccttccag cgtcttcaaa
     3781 gtccttgcac aaattgaagc cacctcgtcc aagcccacaa cttgcgcaca tattcccggt
     3841 ctatcccctc gcaaggcggc aatggcgttt tccaaagcaa ggcgtgcaac ctcagccggg
     3901 ttgtgcaccg caatccatga aggatccccc atagactcga gaacctcact cgccccctct
     3961 atcctgtcgg acgaagtagg atggctcgtg acaggcatct cgggcagacg cgtcaggatc
     4021 cggaatatcc ttcgcacgca atagccgcac tcggccgcat gcaatatcgc catggcgtca
     4081 gcttcgtatt cctgctcata cgatgtggac gcctcacctg aaaacgcctt tgtccccatc
     4141 tttaccaagg ccagtgcagc ctcggccatc gcgccgcctt cctgcgggtt ttccgtgtca
     4201 gcatcaaagc tgccgtctcc cgcactcagc gcccaccagt tgatccagtg gcctgaacgt
     4261 atgtgatgca tttcgtgggc gatgacgagg gcaatctcgt catccgaaga cgtagtgacc
     4321 aagcctcggg taaggtatat cctcccgtcc gcggtagaaa aggcattgat ctgtggagtg
     4381 tcgagcatgg cgattttgta tcccaaagca tcatcacccg ccacatgaga cagtccgtcg
     4441 actattctat ctattctgag ccgatggaac tttgagcccc attctccccc gttttgccgg
     4501 agaaccgcct cgtgagcctc ccttgccaat tcggcctcga cctcctggtt tgcctctaca
     4561 gccgccacgc tggaacgctc gcctataatc ttttggatcc gtgtgagcgg gacatccgga
     4621 aagcccgccg acgagcattt ctgtacatag cgcaccagcg cagccacatc gttctcgcca
     4681 aactgagtcc cgctgcctcc gcccgcctcg gcgagcacag cgcaaagctg tggaggaacc
     4741 cgaagctgca tccatattgc gccgcccact gcacccacaa ctattagaac cgcggccatg
     4801 cccagcgcca gcagtgtcgg cggcaacgcc ctttcatatt ccccgttttt cctagaaggc
     4861 cttgccactg ccgatatttc cgctcaacct ccaacttcgc gtccgccccn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccctagaagc tctcccagtg cagcacctct
     5041 tgaagctcgc gcttgtgaac cgtgcattcc tcatccgggt agccaagggg agagagtgcc
     5101 accagacggt agtctccagg ggccttgcat atgcgcgcta tctccttctc atacggcgtg
     5161 ttgtgccccg caatccagca cgtgcccaaa ccatgggcgt aggcggcaac cagcatgttc
     5221 tgaaaggccg cgcacgtatc ctccacaaag cgcggagtct ggcgagaaaa tgccacaaga
     5281 catacagggg catcggctat gaacttcccg tatgtagcta cagctgctat ctcttccctg
     5341 atcgactgct cagtcactac aacgaattcc catggctgat cattccgggc actcggcgcc
     5401 aacctgccgc aatccacaat ctcctctatg atctctcggg gcacaggatc aggccgatac
     5461 ttgcgcacgc ttcgccttcc ccggattgca tctatagctt ccattttatc tcctcccgca
     5521 aatagtctcg tgcaggccaa acgtgccggg ttcacggcac acacttccca cacaacaggg
     5581 cctcggcccg cctgcaatgt cccacttctt ggagtcgaag tgccgcaagc tatccccgct
     5641 cgcggagctt gcacgatata gccctggcta tctccgctcc aacccgggca ttgttctcga
     5701 gcaatcccag gtttgcctcg accgaccttc ccccggtcgt ctccgcaagc cgggcaagaa
     5761 ggtaaggcgt aagggccttc ccacgcacac cccgctcatc ggcgagcgcc acggcatctt
     5821 caatagccgc atcgatctct tcggaggaaa gctccttctc cgccggaata ggagctgcca
     5881 ccaagagccc gccatggatt cccaattcct ctctagccca aaaagcatcc gcaacttcgt
     5941 cggcggactc gactcgccaa gagagcggaa tcccagaatc ggcagagtag aacgccggaa
     6001 gtttatcagt tctgtatccc agcacgcaca ctcccacagt ctccaggtat tccatggttt
     6061 tgggcagatc aagtatcgcc ttcgcgcctg cgcacacagt aaccacgggc gaacgggcaa
     6121 tgctgacaag atcgtgggaa atgtcgaagg tctcggacgc gccccgatgg acgcctccaa
     6181 ttccgccagt tgcaaacact cgtaccccgg cgcgctcggc gatggcaagc gtgccggaga
     6241 cggtgacccc tgcagacatc ccagaagcaa cgacaaatgc tatgtcgtgt ccagatgcct
     6301 tgacagccct agaggcagca ccgtcagccg ccaggcggtc tatctcttcc ggtgtcattc
     6361 caacatgcac aacgccgtct ataataccca tagttgcagg aacacacccc tcctcgcggg
     6421 caatcgactc cacccgcaag gccaagtccc tgctcacagg ataaggaagc ccatgagtca
     6481 gaacagtcga ctcgaaagcc aacacaggcc tctcctgatt gagcgcatct gttacttcat
     6541 ccttgacaat ccataagttt ttcataaccc tgcccccatg caaccgattt gtgcggcagt
     6601 ggatgccgca gtctgacact ggccccattc ccacctttag gcgcttcaac acgtttcgca
     6661 aggttccttc tcaacaggaa ctcataaact tcgcggaccc gttctttgcc tgctattccc
     6721 ctcatcactt gcaccaggca acttccaagc cgtcccccac aacaggcgcc cgcggaaagt
     6781 cttatccgtc cctgtctttc caagccctgc ccgagttgga ctgaagagca tcgcaaccca
     6841 tgcattcccc tgaactatgc cgcccatctt tcgtttatca ttactgacga tactactttc
     6901 tcagggaggt tgcaccatga aaaccaagcc attcctcgcc acagtcctgg ccgggctatt
     6961 gctcacagca ggcgcactag cgtacgcaaa aaccactccg cccaagactg cagacgctga
     7021 agcggaggga ctaacaatcg aaggctccgg gtcactccag gcacctgcag cgaaactcgt
     7081 tgcggaagtc aagatcctca aggccgtgga tgatctaggc ctttcaaagg aacaggtgaa
     7141 gtccttggcc aaactcgccc acaacctaga cgacgaggtt accaagggca tagatgcaat
     7201 cgtcaaacag ctggaaatcg agaagaacga actgctggca ggtcctgcgg gggacgcggt
     7261 tcacagagcc gaaagcgaga tcgcagacga aaaggctgcc ataggcaaag cgctttcaga
     7321 tgctcagaag gaagctgtaa agatactctc ccccgcacaa cgcagcaagg caatgaagat
     7381 gattcaggag atcgagatcc ccaaagaggc gcgcaaacgc ctcatggcag acgggccaaa
     7441 gatgttgaag gccctatccg atctgctcga tcagaaactg gctactatgt agacggattc
     7501 cacaacgcat acgaccccgg ccgggcgccg atttcaggcc ttcttaggct tgaatcgggc
     7561 ccggcacgac tccctcactc cgctctagcc tgagctcggg cttatccggt atggaatact
     7621 tgtcccatga agtcttcgct attgaatcta tatggtatga tcccaatttc cgatctgggt
     7681 agacttgtaa ccatagctgc taaagaggca ttttgtcccg cactctgact cgagctatgc
     7741 ggtctgcgaa atgctcgtgg tattcgagta atcagtatct ttggccccct cattcgcgcg
     7801 tgcgcctcaa caaaagcctc acgccaacct tccgcagacc tgagattggc actttccgaa
     7861 gtccatagta tcattagact tgcaacgccg aggcctgcac gcgtcgcgct ccgcacccta
     7921 cgtttcgcgt cctgcgctct cggcagcata tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nncacgcgtc gcgctccgca ccctacgttt cgcgtcctgc gctctcggca
     8101 gcatataacg tgccaagctc gtaggcgaac aaacacagtc gagcggtcgg cagacaggca
     8161 agctgcttag gaggatagct atgctctaca taccaaacga ttcgaatgat ccccgattca
     8221 acctggcatt tgaagaatac gtactgaaaa acctcgatat ggatgatccc taccttctcc
     8281 tctggcgcaa tgcccctaca gttgtcgtag gccgctttca gaacacgccc gatgaaatca
     8341 atgccgacta cgtagaggag cacggagtac acgtagtgag gcgaatgact ggcggcggag
     8401 cggtctacca cgatctgggc aacctcaact tcagtttcat cgagcgcaag acctcagagg
     8461 gaatagacat gaaactgttt gcccgcagga tagcccaggc ccttcaggga gtcggcctgg
     8521 atgtggaagt aagcgggaga aacgatctga ccattgacgg acggaagttc tccgggaacg
     8581 cccagtatat ctaccacgac agagtgctcc accacggaac aatcctatac agctcagacc
     8641 tggataacgt gcaggcagcc ctcaacgtca aagcagacaa gatcgcctct aaaggcgtca
     8701 agtcagtcaa aagcagagta acaaatctca tagattacat gcccgttccc ctttcgatcg
     8761 aggaatttca gaaatccctt ctgctccagt tgttccaggg cgcccctgtt cgaacgcgag
     8821 tcctgacaaa tgcggagcta gaggagataa acgagctcgt ggcggcaaaa tatgccaaat
     8881 gggagtggaa cttcgggaga tcgcccgcct acaacgtaag caacagagga agatttggct
     8941 gtggggaggt agccatatac ctttgggttg atgaagggat aatccgatct tgcaggattc
     9001 acggagattt ctttaccgca agagacatac atgacctcga gtcggggctg acggggatcg
     9061 aatgctctaa agaaaccctt agcaaggcat accaagacct caatgtcgca aattacctgg
     9121 aaggtctcgg cctcgacgag ttcctgaagc tgaccacaag caccaaagca gtctgatgat
     9181 cgacttcagc ttgccttccc ctgcacacag ttacgggcgg caccaaagcg ccgcccgtaa
     9241 cacctccgaa tgcaaaaact ctcttgcttt ggcagatcca tgtcagcgca gagagaacgg
     9301 caacatcctc aagtcgcaga gcgtcgcgcc gcctcgttga cagcctcttt tccaagccgg
     9361 agctctatcc aggctacgca ttctcagtga ctatcttcac tccagcactc gcacctatcc
     9421 tgttggcgcc cgcggcaatc atggcccggg cagtagcggc gtcacgaatc cctccggatg
     9481 ccttaacccc catgctctgt cccacagtcc gcctcatcag ggccacatct tccacagtcg
     9541 ctccgccatg cccgaaccca gtggatgtct tgacgaaatc agcccctgcg cgctttgcga
     9601 tgaggcatgc cctgaccttc tcttcatcag tcaaaaggcc cgtctcgatt atcactttca
     9661 caagagcgcg gcctcctgcc gcgtccacca ccgattcgat atcccgcaaa acacagtcga
     9721 gttcgccacc acggagagcg ccgatattca tgaccatgtc aacctctgat gcgccactcg
     9781 cgatggcgtc cctgacttct accgccttga ccatggatgt agcggcacct aagggaaaac
     9841 cgatgaccgt acatacggct acatcagtcc cttccagaag ttcgtatgca aaaccgcagt
     9901 agcacgggtt tacacaaaca gacgcaaacc cgtataaggc ggcttcttca cacagcgtta
     9961 ctacctgatc ccatgtggca tcaggtttga gcagagtatg gtcgatcatg gacgcaagct
    10021 tcgcgtcgac tccttgcggc cgccccgtca ccttagccct gaccgcctcg acgcgccccc
    10081 tagcccgctt gaagccttct cccgcactgc tcacttcgga agagactttc ttgatgagtc
    10141 cgtcaatatc catctgttgc acctccatgc acagtcgatc ccgatcagtt tcaccttctg
    10201 ctactattct ccctcaagtt tgacatccct gcgccccgct tgatagacgg cgtggcccca
    10261 cacgacctgt acgcgtgtga agacaaagtc gcgcagctgt atccctaatg tcgggccata
    10321 tgttcgcatt ctaaacaaag tttgaaggat tgtcccaaaa cgcggagaat agcaccctga
    10381 gtaagagctc ggcaaaaccc gcttctcctc gtcatcatct gtgttatact attgcttgga
    10441 gaatttcggg tctggttttt acgcctacca ttgtttaata cgcaaagatt ttagcatgat
    10501 tcagagcaca agagtaaagg tggacgatgg gctatgggac aatccggcta cagttcgtgc
    10561 tttagcggcg tcaaagccag cgatacgcgg atacttaaga gcattaacct ccttgcagtc
    10621 ctaaaagttc tcagagaaaa ctcgtcggtc tcccgcgccg acattgctcg catcaccgag
    10681 ctcaccccgg ccacagtttc aagcatagtg tcgcttctca taaccgcggg aatagccaag
    10741 gaaacagggt atggcgagtc caccggggga cgtcctcccg tgatggtaga gttcaacccc
    10801 aaggcattct acctagccgg ggtggatctc ggagtgagta agataatctc agtcgttacc
    10861 gatcttgagg gcaatattgt gtccaagacg cgtctagagc tcgacgccca tcagggtgtg
    10921 gactcgatca tggccagcct ctttgaagca acgcatgaaa cattggagca agttagagat
    10981 gtgcgcgaca ggattgcagg actggggctg tctgtcccgg gcctgataga tgctgagcgc
    11041 ggtattagcg tatttgcgcc caacatcccc ggctggagcg atattcccat cgtcaaggtc
    11101 tttgaagatg aattcgccat cccgtgttgg gtggagaatg acgcgcgcgc gatggccatc
    11161 ggcgaggcca tgttcggagc aggccgggga cacgacaaca tcctctgcat aaacgttgga
    11221 agaggcatcg gcgccggcat catcatgaac ggcgagatcc accggggcaa gcaagggggc
    11281 gcaggcgagc tggggcacat gaccatcgac cccaacgggc cagtctgccc gtgcggcaac
    11341 cacggctgcc ttgaggcgat ggccgccgga cccgccattg cagccgcagc catcagggct
    11401 gtttctaccg ggtcgagcac gctcatacgc gacatcgtag acggcaagat agaggccata
    11461 accgccgagg tcgtctccca agccgccgca caaggcgacc tgttggcgac gagactcatt
    11521 caggaggcgg gcaggtattt ggggatcgga atagctaacg ccgtcaacct gctgagcccc
    11581 gacatagtca tcatcggagg aggagtcgcc cgggcaggag acattctctt cgatgaagtt
    11641 agggccactg tacaaaaacg cgcattcact gccatggtca acttgccccc catactcccc
    11701 tcggcccagg gcgaggaagc aagctcagtg ggggcagcag cgctggtgtt tgaagagatg
    11761 ttggcggttg ggagcgttgc aaacctcatg ggggaaagca gctgaacgtg gaagaatgcg
    11821 tcaacggtaa gggaacacgg ccccgcaatc cgcgcactct gcttgaactc cttatgccta
    11881 gatcacgagt atatcaggcc tctgcaacgt caccccgtcg attttcacct caatcatcgg
    11941 ccaccaaccc tcaccaacag taaccaccct ggtctcgtcc tccatcacaa ggaacgaatc
    12001 ctccgattta gccccgggga cagaagggtt ccacgccact gcctgcagcg gcattattac
    12061 gccacgatca gggcccccga cttcctgagg catggcaaaa aactccctgt cctggtagcc
    12121 gatgggccct ccttgatgat ggcgcttcca ctcatcttca aaaccttgag cccgatattc
    12181 ctccttcatg gcctccatca catccgccca tcgtgtgcct gcccgcgtac gggcattcat
    12241 agcagcacac acatctgccg cagcctcgtg ccgccttcgg agcttggggc tgggttcgcc
    12301 gatatggacc gacctagtga gggcgacagt caggccgcca caatgggcgc aaacgattat
    12361 catacaatac ttgtctacaa ggttcattgt gggaagaggg tgcctgtacc gctccacacg
    12421 ccaatctgcg cccacaagca acacagtggg gaaatagccg aaagcgaaaa gatccgacgc
    12481 cactcggcct gctatttcgc gctctgtttg gcccctgcgc acctccctgg ccgcccgctc
    12541 caccacttct gccacaccat gcgccacctt cacgtaccta tcggcttcgc cttttgtaaa
    12601 aggataacgc agctgcacga gctcagggcc tacaaactcg gcgccggcaa gaggaacatc
    12661 cgccccgaca cggccaccgt aactgagcag actggtccca tctacaaagc cctttgccgc
    12721 ctcacctagg gcttccgcct cgccgtacca cggatgctca cgcatgcgga aaatgccttc
    12781 tgcccaaata tgggcgagcg cttcctcgca aaatcgcctt gcttcaacgt tcgacgtcaa
    12841 cacccattgg tcgtcatggg tgatgaggac agacgctacc ccgatgtccg ttgctcccgc
    12901 acacctgttg tctcccccag cggttatcca cgcgaaattg tcgaagcgcc gcaaaatcat
    12961 cacatcgagt ccgcgttcac acatgtactt cctggctcga gcaagtttga cctttatttc
    13021 accacccacg acctcatccc ccatgcactc cgtcatctcg ctcatccctt caaggctccc
    13081 gcaagcaatc ctcggacgaa atatcggccg agcagaacat agatgagcac cgttggaagc
    13141 gcagcaagga tcgatccagc cataggcagg ttccacgaca cagcctgacc gccagctaga
    13201 ttcgccagag ccaccgttat gggctgtgca tccgggcgcg ccaaggtcac agcgaataga
    13261 aactcgttcc atatctgcgt aaactgccag atgcaaacga cgagaaacgg cgggccagag
    13321 agcggcatta tcaagtatct gaagatcccg aagaagtccg ccccatctat gctggctgcg
    13381 cccagcagtt cgtcagggat ctcttcgtag aagctcctaa aaagcagggt agtaatggga
    13441 agaccataga ctacatgcac tagtatcaag cctggtagcc ccccgtacag tttcgtcttc
    13501 tgcatgaact gaaagagggg cacaagtatg ctctgataag gaatgaacat gccgaacact
    13561 atcagcggca ttatgatatt ggtccccgga atcggatact tgcatagcac ataaccgttg
    13621 agagaaccca tgacagctga aatcagcgtt gcagaaatgg cgaggatgaa actgtttttg
    13681 agataagggg cgaactgctt aaatgctgtg gagtagctct cccagttggg cttcgacggg
    13741 agctcccatg ccgtcatcaa gtttatctcc tcaggcgctt tcaggcttgt gactaccgtc
    13801 atgtagacgg ggatcagata agcaatcgcc agaagaaccg cgagaacctg cactagtatt
    13861 ttcgacactc ccttgggttt catcgcttat cactcctttc ccgtcttgaa tgaattgacc
    13921 agataaggaa tgatcagcaa ggaaacgaga atcaacagga tcactccgat agcggaaccg
    13981 acagcaagct cgttcccgcg gaaagtctta aggaacatgg ttatcgcagg cacagacgtc
    14041 ggcacatgat cggggcctgc catggagaag atcaaatcga aaatcttcag ggctatgtgt
    14101 cctagaatga ctatggcgct tacggtgatc ggcttgagca gcggcaaatc cacgtgcctg
    14161 tagatctgga acttgcttgc tccgtcaacc cgggcagctt cgcgaagctc gtcgggtata
    14221 gtgcggagcc cggcaaggta caacgccatt gtatagcctg acatctgcca tacagcagcg
    14281 acgataacac cccaaagcgc cctgttgaaa ccgtgtgcct cgggataatc caggagcttg
    14341 acccgggtca gaatcccgct tgccgaaatc ccgatgagca cggcacatat gatggacttc
    14401 gcgcggagct cttttcgttc tccacggcgc cagtgcccgt aagcgaatac cgccagaaca
    14461 accgcggccg ctatgcagat gtagtggaaa atgtgggacc aatcgaagat aaggatctgc
    14521 tcgcggctgg aaagccatag aaagtcgcct gcgggcaatc cgatcaaggt aggcaacaca
    14581 tttatgcccc ctctcggctg gagcagccag cgccagatcg tccccgtaac taccagcgac
    14641 aatgacatcg ggtacaggaa aaccgttcgg aagaacgcct caccccagat gagctgatcg
    14701 ataagcgtag ccaaaagcaa tcccacaagc aaggacaggc taacgaagag caatgtgaag
    14761 aagaacatgt ttgtaatgtc ctgcctgaac cgcatgttca aaaagccccc gaacagctcg
    14821 cgatagttcg ccagtcccac gtgatggatc tcaggatcca gcgacatcgc cgcttcctgg
    14881 ccccaatccg tcatcgacac gcgcacagtc tgagctatga atccgtacac aaatactgcc
    14941 aagagcacta tagacggcag gataatgagg gccgccgtca actggtctct tcgtgattgc
    15001 atgcccttca ccgtccttct tcttgcaggc gcagggcccg tcgccctcag gcggcgggcc
    15061 ttgcgcctgt tctatgtaga acaaccttcc tacttcccta ttccggcctg aatcgccact
    15121 gcctgggcag cgtttgctgc agcctgagcg ttcttcgtgc tcaggaagat gtccatgacc
    15181 gtggcaaagt cattcatgaa cctctcattg gccacgactc cgtgagccaa agagccgacg
    15241 atcttattcg tcctccagtc gcgcgcagcg ctttgcagat atgcatcgta tttcgtcaga
    15301 tccgagtcgg ttctggcact gatcgagccc ttaagcgggt tgaaggtgtc ctgcccgtca
    15361 cggcttccga gcatcttcag ccatgcaatc gcattctcct tgttcttgca accaaccggg
    15421 aggccaaacg aatcagagag catcatgaat atgcccttgg tgcccgggga tgccgcccac
    15481 ccaaacccgg ttccgggttt gagcttgagg gtagttgtca tgtatcccga agcccagtct
    15541 cccatgatgt tgaacgcggc ctcacccttg accaccatat cagtggcctg ctgccatgag
    15601 agggatggcg catcggtatt agtgtacttc aagatttcgc cgaacagttc ccacacccgg
    15661 atgacttcag ggcttcgcca ggagagtttg ccgttccaca gctgctccca tttgacaggg
    15721 cctagcaccg atagggctac actttcccac aggtgagtgg cggtccagtt ctcgcccact
    15781 gacagcggaa taactccttt tgccttgagc tttggcgcga tctccaagaa ctcatcccat
    15841 gtatttggca cgccaacgcc ccatttggca agattgccgg gaacgaagta caacacgttt
    15901 gaacggtgga tgttcacagg caccgaccat attcccttgt cggtgctgag cagattgagc
    15961 aagtccgccg ggaatacctt gttccagccc tcctgctcat acagccacgt aagatcctcc
    16021 atcctgttgg ccacaaccca agtgccgatc aattcctggc ccgcgtggac ctggaacgta
    16081 tccggcggat cgccaccgag catcctggtc ttaagaacgg ccttggcatt gactcccgat
    16141 cccccggtca ccgtggagtt gatgacagtc gctcccggat acatcttctc atacttgctt
    16201 atcagcgctt caagagcagg gccttcgtct ccagcccacc atgagaagat ctcgagtttc
    16261 ccacggacgg ccgcatctgc ggctgaggaa aacatcagaa cgatcgccgc gcacatcgca
    16321 gcaagcttga accatcttga tgtcctcatt catatccctc cgtctttaat tgatacagct
    16381 ggcaaatcca actacaccaa acgccgcatt atcatcaata acgcacgtac cctcccggca
    16441 ccacctccat gacagatcca gtcattcctc gatccttgcc ccatccacca catccactac
    16501 aaatgttttg ggttgcacgg ctccgtaacg catcggagcg tcaggaagcg cttccatcag
    16561 gtgaggcaag gcttccaagt gcacgagact cacagtgcat cctccaaagc cggccccggt
    16621 cattcgcgct ccgaggactc catcggtctt ccaggcaatt tcaaccgcca tgtccagttc
    16681 gggacagcta accccataca aatccctcag actctcgtga gactgccaca tcaaatctcc
    16741 gaaagctcca agatcacccc gttttagcgc ctccacgccg ccttgaactc tctcgttctc
    16801 ggctatcacg tgttcgcagc ggcgggccac tatagagggg agaatcccct ttatctcgtc
    16861 aaatagggca atgtcgatat ctcgaagggt ccggatgtcg tcgcgcctca atgcccccga
    16921 tatcatcctt gtgcccagct ctacctcttg cctccggtga ttgtactctg agtcgacgag
    16981 cccgcgcctt atgcccgtgt cgtagattac cagcgccgcc tcggcccggc cgagccccag
    17041 ttctacctct tcatgccgga ggtttctgca atccaagtag actgcgtgat ccgcccgtcc
    17101 catgcaggat gccatctggt ccatgattcc gcagttaacc cctacaaagc tgttttcagc
    17161 gtgctggcag gccaaggcta tccaacgcct aagctcgagt ccgcatcggg attgggtagc
    17221 gttgataagg gcaacatcga ttcctgccgc agcaagaagg gaaatggcgg aagccacctc
    17281 gaaagccgcc gatgaactga gccctgcccc ttcgggcaca tcgcctgata tgactgcatc
    17341 aagtccgcaa agcctgagct cgccctcgtt gcccgagaac ttcagttcca cttttccctt
    17401 gtattcgcat ccctcaagag cccatgctac ccctcggaag tagttgctcc acccggcatc
    17461 tgccgaagga aggatttcgt ccagcgaaaa ctgggtcctt tgatgataat cacacgaata
    17521 gaggctcaca ttcctgtcgg tgcgcgcact gaacgcgatg gcgacatcgt gtcctatggc
    17581 cacgggtaac acaaatccgc ctgaataatc agtatgttca ccgatgaggt ttacccttcc
    17641 aggcgcccgg gcgatatgaa cctcgccgtc tgcaaactca ccgaactctt gtcggaactg
    17701 gtcaagaagc cacccgctat ctaccgccac cagctctgcc ccctctgacg cctccttccg
    17761 tcgtttcaca agaacaggcc aaagccgcat cagcagaagt tgcgcgttcg atagctgtac
    17821 gcagttcttc agctgtctcc tctggaagcg tgtcgtttat gaaagtgccc cctcccgact
    17881 cgcaacccgc caggtatttg attttcgtgg cagttcgatg aagggggtag aactctatat
    17941 ggaaatggca atggctccag tcgcctttcg cggtcggagc ttggtgcatg atcatcatgt
    18001 aaggaaggga gaatccaaag aggtgatcgt aaccgcgcgt cactgatttc aagatttcag
    18061 caagcgatct cctctcatcg ccgtcaagat cgggcaagca tgacctgtgg cttttcgatg
    18121 taacgtggac ctcatatgga tatcgggcgt agaaaggcac gacagccgcg aaatgcgagt
    18181 tgccggcgat cacccgcctc ccgtcatcaa gctcctgctc aagaatgtcg caaaaaaggc
    18241 accttccggt agcgtcgcgg tactcttcgg ccgactcgag ctctttcgca ggtatcggcg
    18301 ggatatatgg gaacgcatag atctgcccat ggggatgatg gagggtcact cctacagcat
    18361 cccccttgtt ctcgaatatg aggacataat ctacgtaatc gatcgatccg agctcgctat
    18421 atcggtctgt ccatactgca ataaggtttg tgatgtggtc aatatcgagg tctgcaaatg
    18481 tggtgtgatg ttcgtctgtg tagaggacta cttcgcaaat gccctgtgcg ggttggactc
    18541 tgaggacgcc ggtgccttcc actgccggtt caggcggaac atgccggaaa gaagggaact
    18601 tgttttcaaa aaccacaatc tcgtagctat aatcaggcac ttccgttgga aatgatgtat
    18661 cccgtgtagg gcacaacgga cagaagtctt cgggaggctt atacgtcctt tcctgcctgt
    18721 gggttgcagt tatgacccat tgcttgagca cagggttcca ccgcatctcc gacactgtcc
    18781 gtcacccctc cctcatcatc tgcccgaata gctagatact catccccgcc tttcccgttg
    18841 tttaggggga acgagtaata tatgagatat ctgccgtcat ccttgcgtat tttcttctta
    18901 atcattgtcc gtgctccctc atgaaatctt cactataatt tagcttctaa acaaagatca
    18961 caatcaattc cgtttgctca tcccttgttt ccggtaattt ccgagaccat tcgaggcttg
    19021 tacaatcctc ttttagcagt atagttctat gccagtcata gaattcctgc acattaacaa
    19081 taatcactcc tgcgaacgcg atggaccatc tctcccgtat cgcccggaga tttgcatagg
    19141 tcgctaacaa agtccttgcc agtctgtgct gtgttttcca tgtatatgca ctatgcccgc
    19201 cagaaggccc tcttttcggc cttaacatgg caatccgact cagccgggac agaaggggat
    19261 tgttcttgaa ggggttggtg caaataggcg catcgcacac gtgaagctgc cagtttgccg
    19321 agacctctat gtcatcttag ccggtcgctg gctaggatct tgctgccata gccacctcga
    19381 aggtttacct tctccctgcg aaaggggatt ccacaagccc tgcccgcatc ttcatgattc
    19441 gacattctct ggtatccgaa tacgaaccca agccaatggc tttacagccc atccgacgag
    19501 tcctcgatat ggcgatagcc gcccattttg agtgtttggg gcagcatgcc tcgctctgct
    19561 cgatcttgca tcttgcccac tgcttccgaa gactggacgc gcccatgcaa acagatcctt
    19621 agatcaacga gctagaatac aggctcggcc gacgctgccc caaacaaagg gggtcaatcc
    19681 tcatcagtca accacactcc tccctctgtg gcccgatgaa tctccctggc agcctccacg
    19741 ccttccgcca cgccgccgca cttgagcatg agaatcatcc caaatcccca gcgccgggca
    19801 gcccggaccc tgtagatggc ttcatctgca ttctctgccc aagcatgcca aatgcccctg
    19861 gatcgcctct cgtcttcacg caacggcttt tcctcgcccg ccccgcccca atttgggcag
    19921 ccgtccacag cagtcagagc gcttcctatg gaatcgatgc tgttgtagtg gctatcagcg
    19981 tcaaagctca tgaagccctc gaggttcgcc acttccatga gattttgctc gtaccccctg
    20041 cagcaatgga tggatatgcc tccgaaagcg cgggagagac gctcgttagg ggctttcatg
    20101 aactcgcggt aatgctgggg cgataccacg gctgtcacat cgtcgctcac atagatgcct
    20161 cgtggccgcc aaaggttgac atgggtatgg ccgaagaaat tgaccgccct cgcttcctgc
    20221 atccggacga actcgatcag tgaatcggtc accatttcga gaaggaagtg aactgcatct
    20281 ggagcggtgt acatcgctat caggagttcc tcgtaattta ggatctgagt cgcagtatcc
    20341 ataggagatt gcacgtctgt gagcctaatc ggcacagtgc cccgggtcat ctcggagaaa
    20401 tagccgatcc tgtcgagcac cttggcggca agtcccgatc gcgccaagtc aggctttttc
    20461 agccgataca catcgtcggg gtcatctgtg atagggatgg tccacgggtc ttctccggga
    20521 gttatcctta tctcacaacc gtatgctgag gggattacgc ccacgcccat gtacggccat
    20581 atcgatggaa tattgcaaaa gcctgcccga aggcaggcga gatcccaatg agcggaagcc
    20641 aactggtatt ccagatcctt ttccggatct tccatcctct cggccaccga gtagtcgggc
    20701 agcgctggat tctccggggc aagcagaatc acaggctcta tgcttcttcc ttcccacaca
    20761 tcagcaagat cccttttggc ccgagccaca aagtcatcgg ggatgtccat agtagtgcag
    20821 ttcatttggc ccatctacac ctccgcgatg cttcaccctg tcccccacag gaccaggtgt
    20881 ctgtcaagca gactgaatat aattcgcgag aagtaccttg gcctaaccgg gcggctaggc
    20941 tctgaattgc gggcaatgca gacccaaact tatcgaggtc tgctcagtca gatggcaatg
    21001 gaaccggcgt gggcagcacc gccccaccaa gatcgagctt ggcatgctga tcttctatga
    21061 ccctgaggta atctgcaccg ctctgaattt gctcggcaag atccccgatc cctgccttga
    21121 gctgtgctaa atggaggatg tcaaggatca cgtgttcgac tgccatctgc acaatcatag
    21181 gatttgtcgt tacaatacca aggttctcat catgcctgat atcacccacc agcacattcc
    21241 tgcaatcggt tgccacaata atgcgccggt tcggccattt ctccttgatg atgtcctctg
    21301 caagcccata ctcgtatatg gttcctaccg agaatggaac cttcgtgtgg cagaatatga
    21361 tcacggtccg tcccgcgccc tccgcagcct ccagctcctc cttgagagga acacagtcgc
    21421 tcccccaagc agagacaacg acctcatgct tcgtcccgcg gataagctca cgggccttgc
    21481 ggatagtgtc ctcatatccg gccacgccca gtatgtagct ttcctccgac acgccggact
    21541 ccatcttctt gagagaacgt tccaggttgt cgaggtttct atcatagagt tccctgtatt
    21601 ccttcaacag ctgagaaggg ggcaacggag cgtacatcac cggatcggaa tcgacttgca
    21661 gaacgaatcc tttctcgaga agcctgtcga ggacgccata aacgacagac ctcgtgatcc
    21721 ccgaccgctt gctcacttca taaccgttgg cagcgccata gcgcagaagc gcttgatacg
    21781 ctttggcttc attaagggac aagcccatgt cctgcagggt ttcaacaagt ccagccaatt
    21841 tgctcttgcc tccagacgaa aaatggggta gtagtaatat tgactactac tatcattcta
    21901 ctggccgcgc ggacgcaatg tcaacgactt cgacgcaatc gccacgaagc catccgagaa
    21961 ggatggagaa tctatgtggc ggacccggtt tgccagcatt ttcgcataca atcatgcatt
    22021 ggtcttccgc attcccgcta ttcttctttc gttcaaggag attgcacggg agccgcagaa
    22081 cgttgaactg agaaacggag gtttgagcaa gtgacctgtc tcgacgacat cttggccgat
    22141 ggcgcgctca cccgcgtcct ccgatccttc aaagaagcca cggggatagc ggcgcgcatc
    22201 gtaaaagcca ccggggcgcc agccatagcc tcccggacat gggaagactg cgccttctgc
    22261 cgcctggtcc gttcctccga ggaaggccgt aatcgctgtt cgatgtcgta tgcatatgcc
    22321 gcaaggcaag ccgcatacct cggagatctc tacgtcttcc agtgccatgc cgggctcgtc
    22381 tgctgggccg cgccgctggt gagcgacggt accctgctag gcgcggtcat ctgcgggcag
    22441 gtcatcatgt gggagccaga cgaatatctg attcgcgatt ccgtcgaacg cctccgcgat
    22501 cttgatattc ctcttcctca acttgaagcc gccattaccc agcttgagca ggtaacaacc
    22561 cgccgcgtcc aggccgctgc ggaactgctc ttcgcagtat ccacttatat agtgcgctcc
    22621 gaggacctag cactcgaaca gagaaacgag atatacaggc aacagcggct cctcggtgaa
    22681 gccatccagg aacgcaagcg cattgaagag cagttttcca tccgcggcct ctattcgcct
    22741 cagcaagaaa gggagctcct atcggcagtc cgaagcgggc agcgaacgga ggcaaagcgg
    22801 atgctcaatg agatcctggc cgatattttc ctgagtcaac ctgaccgccc ggacatcatc
    22861 aaagcccgtc tcctcgaact tgccgtcttc ctcgcccgcg ccgcagtgga agcaggcgcc
    22921 gaccccgaac gaatactcgg cctcaactac cagtccatcg gagatctgtc ccgttgcgag
    22981 actttcgaag aggtgtgttt ctggatcgtg cgggtaatgg acgagttcat ggatgccgtc
    23041 caagatactc ctgatttgcc ctcagggcaa gtacaggagg ccattaggtt catgcaaagc
    23101 aacctcgctt ccaaactgtc tttggccgac atagcatcat cagtccatat cagcccctcc
    23161 catctgagcc atctctttaa ggaggaaaca ggcatgtcgg taatggactg cctcacagcc
    23221 ctccgcatta gcgaagccaa acacctcctg gccatgccgc agctcacagt ggcacaggtt
    23281 tctgccgcag tggggtttca agaccccgcc catttcagcc gctcgttcaa gcggatggaa
    23341 ggaatctcgc caacagccta tcgccaaagc atgatttcag caggataatc catgaaacca
    23401 caagaccgtc catggacttc atttgccatt cctctaaaat gggagttggt gaactatctg
    23461 acacaagggg gttagggcat tgcatgatta cttagaaatg agagaagctt tggaaagtgg
    23521 aaatgcgccc aaggtaaagg cccttgcaga gcaggccctt gcagaaggcc gaagcgcaac
    23581 tgagatccta aacgaaggat tgatcgccgc catgagtgtg gttggcgtca agttcaagaa
    23641 caacgagatt tacgttcctg aagttatggt ggctgccaga gccatgaccg caggtctcaa
    23701 cgtgatcagg cccgtgctcg ttgagaccgg ggcgccgtct gagggcaaag tcgtaatagg
    23761 tacagtcaaa ggagaccttc atgacatcgg caaaaatctg gtcaagatga tgtttgaggg
    23821 agcaggcttc gaagtcgtag atcttggggt cgacgtttcc gcggacgcat ttgtcgacgc
    23881 agtcagaaac gaacggcctg atatactagg gttgtcagcc ctgctgacaa cgactatgcc
    23941 agctatgaag accacgatcg atgcgctgga ggaagcgggg atcagggacc aggtaaaggt
    24001 gatcgtaggc ggcgcgccgg tcacagacca ctatgccagg gagataaaag cagatggtta
    24061 cgcagcagac gcaagctcgg cagtggatct cggccgccgg ctgatctccg cataggggga
    24121 cacgatcatg aaactgatag aactggcaat gtcaggcaaa cgaaggctcg tcgccccgct
    24181 cttgggctac cccggagcaa agctgactgg ctcctcactc aagcagaacg aattcaatcc
    24241 cgcgctccat gccaaaacca tggatagaat cgcggcgcgg ttcgctcccg acgcagtttt
    24301 cttcatgatg gacctgtcgc ttgaagcagg cgcgcttggt ttgccggtga gataccctct
    24361 ctacgagtcg ccttctgtag aggaacaccc aatccgggaa gtgtccgacc ttgacgcgct
    24421 tcgcgtcctc gacccgcttg acgacgcaag gcttgcaggt tacatcaaga ccatggagct
    24481 gatgaagaga agcatatccg tgccgtgtgc ggcttacgtg gccggaccgt tcaccctggc
    24541 gggattgctc atgggcgcat ccgatattgc aatggcgacc atcaccgatc ccgaattggt
    24601 ccatgaaata gtcaggttcg cccaggacat atgcatccgc tattcacgcg cgctcaccga
    24661 ggcgggagca gatatgatag tagtgctcga gcctactgcc acattcctct ctccccggtc
    24721 gttcgccaca ttctgcggca cgtacgtccg ggaggtgttc gaagcgcctg gcatggccga
    24781 catgatggtt cttcacatat gcggagacac aacgcacatc cttcgcgaga tgtgcgctac
    24841 cggagcccac gggctgagcc tcgatgctcc tgtgaacatg cgtgcagctg caggcgttgt
    24901 agatcctgat gtcgttctca tcgggaatgt ggaccctgtc ggagttatgc tgtatcgaga
    24961 tgcagccggg gtcgcgcaag ccgttcgcga actcctcgat acaatgtcag gcgtcgagaa
    25021 cttcattttg agtaccggat gcgatctgcc gtatgagacg ccccacgaaa acatcgacgc
    25081 attcatgagt gaaggaagac gataagaatt ggcctctatt atcctgtcta cggaaaagat
    25141 gaagattcca gctaaccccg gggatactat ctcctcggcg ctcggtcgtg caggtgtgca
    25201 cgtatacgcg ccctgcgcgg gtcggggcgt ctgcggcaag tgcaaggtcg tagttgaagg
    25261 agggctcgga atatccgcgc ccaccgagat cgagctcgcg catctttcgg ccaacgaaat
    25321 cgcctcaggc gcgaggctcg cctgccaggc tactatcttg ggggatgcgg atataaccgt
    25381 cttgtcccga gtcgcgccgg gcaatcaaaa cgcctccata ctgacggaca gcaaatggct
    25441 ggacaatcag gttgcgtcag ccaatgcgcg cgttgaagcg aaaaccgtat ccgttgaccc
    25501 tacgccctct gcccgctcag actgggagcg gatctcacta gcttttgggt tgccaaagta
    25561 ttgccaaatc gccgacggga ttttatctga gtgcccagag ccagcctcaa tcccgaccct
    25621 caaacttgcc cagtctgtcg gagaagcaat ggccgaagcc acgagtcctg ctcagtcggc
    25681 aggcacatcc tcctcatcca tcgtcccctg cgccgacaac ggcatcgccc tgaccggcgt
    25741 cttcatcgac ggaaaactgg cagatgtttt cgctgcgaca gccaagccaa cacggcgtgg
    25801 tgaaagtggc gggcggcatc ctctcggcat cgcgctggac atcggcacta ccaccttagt
    25861 ggggtacttg gtgaacttgg cgacaaacac cgtaatcggc gtcaaaggaa ccgtcaaccc
    25921 gcagaccgcc cacggcgccg acctgatctc ccgcatcgcc tactcgcagt cgaaagaggg
    25981 gcttgagact ttaaggctag aggtcatcga ggccgtgaac gagcttgcca acggcctttt
    26041 ggctgacgca gacgcgtcgc ctgacgacgt ctacttagtg ggggccgtcg ggaacacatg
    26101 catgcaccat ctgttctgcg gtatctcccc gagtaggctg gcaaggcttc cttacgctcc
    26161 ggcagttctg gaatccgccc catgctcgcc tgcggaagta ggccttgcgt gcgccaatcc
    26221 cagaggcaag ttcttctttt tgcccaacat cgccgggttc gtaggatccg acgctctggc
    26281 ggtggcactc gtttcaggca tgagtaagag ctcagccccg acacttgcta tcgatatagg
    26341 cacaaacggc gagatcatgc tcgcagcgaa cggaagaatc ctcgcgtgct cgactgctgc
    26401 agggcccgcc ttcgagggag tcaacatatc ctgtggaatg atcgcggccc cgggggcaat
    26461 agacggtgtg cgcatgggca aagatgacat cgagtgccaa gtaatcggag acgcgagccc
    26521 tcgggggata tgtggttcgg gattgataag cctcatcgca gtccttcgcg aagccgggat
    26581 gatctcggaa acaggggctt tcgacccgga cagagttgat ccgggaagtc ctcttagaag
    26641 ccgccttatc aaggaccaag ccggcctcca attcgtgttg gccaaatcgg ggtcacaagg
    26701 cccagatgta gtcctgaccc agcgcgatgt gcgcgagctg cagttggcca aggccgccat
    26761 tagggcagga gtcgaaatac ttcttgcaga aatcgggata gaagcggaag agctttcgca
    26821 gatagtcctt gccggagctt tcggaaccta tcttgatacc gaagccgcca ctgccatcgg
    26881 tttgcttcct gatgcgggcg atgcccgtct ggtctctttg ggaaacgccg caggccaagg
    26941 cgtaatttca gcattggtaa gcgctaaagc atacagggag gcgcaaaacc tggcaaaaac
    27001 gatcgagcat gtggaactcg gagccagccc cctatttatg gaggcattca ccgactccat
    27061 gcttctttcc agaggataac agctggggct cagggcagag ccaagcgctc acacgagccc
    27121 cgcagaacag acggaggcat cgatcgaatg gatgatttcc tcttgaaaca gacggtactg
    27181 actttaaaac ccgaaccggt tcctgcggag gatgtcctca ggtccttggg aatgtccgac
    27241 gtaatcgtca agctcgaggc tgacaactgc ctgcctccgg atattagcaa cgatgtcgcc
    27301 ttgttagcca cgctagccgc catcgatgag gctaacaggg ccgtcatcga cggcctcgtc
    27361 gtcccgcgcg tagtggccac gactctcccg atagcctcca tagccgacaa aggcatcttc
    27421 cttgactcaa tcgccgagaa tagcagcgcg ttcatcccct gcagtggcct gctgttcgaa
    27481 ggtgcaagcc atgtgatgct agcgatagct accatagggc ccctcctcga gcgccaagtg
    27541 aaagcggagt tcgacactgg cgacccgctt gcgggaatcg tcctcgatgc catggggact
    27601 atctccctcc aacgccttgc atcgcgtatc cgccaaacct gcgcccagcg cgcagacgaa
    27661 tgcggaatgg agacaggtcc tcgtatagcg cccggatgcg aggctatgcc tttagatgcg
    27721 cagtcggccc tcttctctct gcttgacgcc gacgccattg gcgtaaccct cagcgattca
    27781 ctcttgatga cgccgctcaa atcgacttcg ttggtcttgc cattagggcc aagcctcccc
    27841 gaccatctca caagattcaa catgtgcgat acatgtcctc acaaaaacag atgcgcaaac
    27901 ataatccttt gatgctaatt cgaatcagtg ccgatcagtg accttaccct atctcaaatt
    27961 ttctcaccta ttaccagcct gcccaggcag gtgacgacaa ttggacaccc ggttatccac
    28021 aataaggcgg cgcctcttaa gaacgctcgg aaactggcaa atgtacttat agcgagaaca
    28081 gtcttgctgc attcccccac atcactgact tgagttcgcc ttcgtccagt ttcgactcga
    28141 gcgcagccct gacccggtcc cgcctgtagt ggcattcccc gccaagcgga gcgtctgtcc
    28201 cccaaagaat cctctcagcc ccgatccgca aaatcgcgtc cttcacatct tcaggcctag
    28261 cccaacttgt atcgaggcag atgttagggt aggcctcagc cacacggata gctgcttccg
    28321 caggtccaaa taggttcatg tgggcaagta tgatccacac atcgggattg cgttcagcaa
    28381 gtgccatgta gcgttcgggc gcagaggact cgtcagcagc acagtgaaac agagctggtt
    28441 ttccctcttg ggcacagatt cggagatacc tgtcaacctc gttcgaatcg gcccgatacc
    28501 ctcctgcagt cggatgaaac ttcagaccgc gaaaccccaa cgcaagaagt tgtcgcactt
    28561 cacctacctt cgtcacttca ggagtagtcc acgcaattgg caccaactcc cgatactctt
    28621 cgacccactc agccaactcg aaattagcct gcataggagc gtcaacagct gagatattcg
    28681 atacaatcgc gagatcgacg tccgcctcat gcatgcattt aacgagctcg gcaggggaat
    28741 acgagacatg tttcttgttt atctggtcat agaattcgcc gacatggcaa tggcaatcgc
    28801 agactttcat acaatccctc cagttcaggc cgccgtgacg gacatgcctt tctattacag
    28861 ccttcgggtt ttcaatggtg ctattgacat cgaatggata tgctaatatt attatagtag
    28921 tcgttaggat gactactagg catccatgca gcttaaatcc gtaggcggca taatccctgg
    28981 ctcaaactca ggtccgagca agcggaatgc tctcatgctc cacgggatct gttgttgcaa
    29041 agcccgattc gagcaagtat atggatcgga tccacaatgn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnnnnn nnnnnnnnna cactaatata cacctttcct tcactgctag cccttttcgg
    29221 accgatcctc aaccggtgca atttcgagca cgacatgaaa tcatatctca actcatcatc
    29281 ctgtttttgt cgaccgaaaa cacattgtct ccgttctcat gcggggcgca atctgataaa
    29341 gccaatttgt tgctgcgagc aagccatact ggcgcacttg ccacccacgg gcgtggttac
    29401 gagtttcact caatactgaa accacagagg aggaaagtta gcatgaaggt taaacgtctg
    29461 tttctctccg tcgtttggag tgcgttgatt ctatcactcg ccatcatccc gtctatggcc
    29521 gcagatccct tggtagtctg gtgcgagccg cagaagatgg gaactatcga accccttgct
    29581 gatcagtgga gcaaaaagac aggcatacca gtgaaggttg aacccgttgg cgtcctggaa
    29641 accgccaaca aggtgcagtt agctgggcca atgggcaaag ggcctgacgt gttctgcagc
    29701 gtttcgggtt cgctcggcat gttagtcgtc acaggaacag ttgccccagt aaaccctaaa
    29761 accttcgatc tgtccaactt catgaaggta agcgtcgatg ctgccacatt caacggcaag
    29821 ctgtacggag tgccctacga catctccacc gtagcaatga tatacaacaa gaagatttgg
    29881 cctgaaccgc ccaaaacctt caacgagatg atcgacagat cccgcgagct tcggaaaagg
    29941 ggcaaattcg gaatcctgtg gccaatggaa agcttctact tctcctatgc aataatggca
    30001 ggatacggcg ggtacatctt tgcgcccacc gagtcaggat gggatacgaa agacattggc
    30061 cttgccaacc caggctctgt taaagccatc aagcttctga agacgctcag agatgaaggg
    30121 atcatcccgg tcggaaccga ccacgtcgtg gcccctgcaa gattcagtga aggcaacgct
    30181 gcagccatca tcgatggctc ctgggcactg cccggaatca agcaggccgg tatcgattac
    30241 ggtgtagctc cgcttccaaa gcttgacaat gacaagtatc cagcgccgtt tgtaagcctg
    30301 aaatggtggc acataagctc gtatagccag cgcaagactg atgctgcaaa cctcatctcc
    30361 tatctaacaa ctaaggaagc catgtataag tccttcaagg ccgctgaagg tatccctccg
    30421 cggcttgacg tcctccagat gcccgatgta aagtcaatgc ccgaagtggc aggctatggc
    30481 gcgcaagcca actacggttc gatactacct gacataccgg aagtcaatcc cgtatgggca
    30541 ccgatggaca gcgcacttga gatcgcaata cgcggcgata agagtgtgga agcagctctt
    30601 tccgatgcgg ttaagatcat cagacaaaac atagctgagc tcaaatagag ccgggcagtt
    30661 accgaacaga atttgacaca acccgcccga tgcgctggca tctgtaaatc cgtcagagca
    30721 gcaaaaacag atgccggcgc atcgggccta actacccgct tagtcctatc agaaggccgc
    30781 agtatcttcg gaaggagtat gctatatgga aaaaatactt atgatgaccg gtggaggatt
    30841 tcacccgttt cacctatgca gcagcatagc aaaagacttt ttggagcaaa cagggatatt
    30901 tacagttgaa atcactgagg atcgagataa gttcctagat ttgtcaaatt acagtgccgt
    30961 catagtctat actcagggcg gtgagctaac cgccaaacag gaaaacggcc tgctcactta
    31021 tgttcgaagt ggtggcggca tcgtaggtat ccactcggct gccgattctt ggaccaataa
    31081 taaaggctat atggaaatga tcggcgctca gtttagagag catggtccga tgacggatat
    31141 caccgtcact tacagcaaag aagctgatgc cgttatccct agagctgata aaagctttgt
    31201 cattcatgac gaattctaca tgcttaagcc gcatgccgaa gaagcgctaa agccattcat
    31261 gtatggtagc tggcatcaag cacaagagat cctgggctat gttcgcgact acggaaaagg
    31321 gaaagtgctt tatactgcac taggacatga tgagagagct ttcaacgttc cacagtttca
    31381 agaactgatt atcaagggtc ttatgtatgt tattggcaag ataagcgacg aaacaattcg
    31441 ttttggcatt gttggttatg gacccctata tggaatgggc aagtatcatg cagatagcat
    31501 caacaatact acagggttga taacaacagc tatatgcgat aaagaccctg cccgcctacg
    31561 agcggccaaa aatgagctcg gtgatatccc cacatttacg gatgcctcgg aaatggccca
    31621 aagcggcctt gttgatgccg cagtagttgc agtccccccc aatgcgcatg ccgaggttgt
    31681 gttggctttg atagagagcg ggctccatgt agtgtcggag aaaccttttg cgataacaac
    31741 agctgaagtt gatcagatga tagaagctgc ccaatgcagg gatgtagttc tctccgtctt
    31801 ccataaccgc cactgggatt cggatatcgt cacgctcagg cagattatgg acctaggctt
    31861 gatcggcgaa gtgtactccg ttgagtgcaa tatggtctca tatcaaatgc catatccagg
    31921 ttggcgaagt cagaaggaag tttccggggg tttgctgtac gatatgggag ttcaccaatt
    31981 tgaaaaagcg ttccagatag ctgcgtacaa ttggggacaa gactgccgaa gtcgcaaagc
    32041 ttttgtttat ggcaaccgtc tcaagaaagc ttggcatcat gttaacgtcg aagactactg
    32101 gagagcatat gtaaaattcg aatccggtct ggagatgcag gttgttcaat caaatctctg
    32161 cgcggcacca aagcccttgt gggttgtact tggaacccgg ggagcagccg tcatagaaag
    32221 ttgggattgg gagaaacctg caatcgtcaa tacatacgtg aacaacaggc ttacgagagt
    32281 tgaatatccg attattccgc cttgcagtat tcctgattac ttcgacaggc tggctgaaaa
    32341 atactatcga aaccttgcag accatgtgct tatggatctg cctctgatca tcactcctga
    32401 gtgggcaaga aatcccgttg cagctattga gctttcagag aaagcttctc tggaaaacag
    32461 ggggctcgaa gcggaattca ttttctaagg ccaatacaga ttttcatgga tattcttcca
    32521 tttcggctca tgattccaca tacatgctca ggttacagac gctgcctcct aattggcgat
    32581 attgacatcg gggatgtata cgaatattat tatagtagtt ggtgtcatga ctactaagta
    32641 accgataagg agacatgtct tcctgagcac gatcttaagc cattacaagc ggggtgttca
    32701 catgtttcac gacatctcgt actacaaacc cgacctccat gactgcataa accgggttcg
    32761 cgattttttc agcctggagc ctggagcacc ccaggcgctg atatacacct tcccctcagt
    32821 gctgaccctt cgcggacctg ccctaaacca gtacgacttc gagcacgaca tgcaatcata
    32881 tctcgacgat tatctcgagt accaggcaca gctaatggag atccgcctcg ggatcgaaga
    32941 cgactggata ttgtcggtcg tcccctacat gggcatcggg gagttcagcg ccttcgtagc
    33001 cggcgagata gagtttggcg aggatacgag ctgggctgct cctgttatca acgaaaagtc
    33061 tgatcttgaa acgctttgcc ttgatcctaa caataagtgg ttcagaaagc ttaacgaggc
    33121 tacccgttac atggttcgca agatagcgcc ttaccgaatt ccgcttggcc gcggttacta
    33181 ttcccccctc gaccttgcct gggccctcag gggcgaagcc atctacatgg atttctacga
    33241 agacccccag ttcgtccaca acttgatgga atttgctcta tcagcaacag aatggtttgc
    33301 aagggctcaa gcggccgaga tctttgcgcc ggacgtggtc catgagcttt cggcatggca
    33361 ttgcggaccc aatagggttg cagtttccga agatatctcg agcctcatct ctccaagcca
    33421 ttacgcacaa tatggggcgc cttacacgca gaggctcttt gactcctttg gcataggcga
    33481 ggtacattgc cattcagccg ggccacatgt agtccccgag tttctgaagc tcaagagagt
    33541 gcggcagatc cagatcgtcg ccgatccaaa cacccgacgc ccggtggaaa tcctcgctga
    33601 cctgatggat aagaatcctg aattattcgg caaatcacct gatgttcctg tgatagcagt
    33661 tgacgcctcg ccacaggagg ccgtcgacta ctacgatctg tctcgacaaa cccgcatcgt
    33721 tttttctgtg gtaactgaaa acgcagaaga agcgcgacat gcggttgaca tattcagggc
    33781 gaaacagaag caatgttgat cggttattag aggttaccgg ggcatcatcg tcgaaaggaa
    33841 cagccacaac acgcaaggtc cacaaaaggc aaaggcatct acaaccatag cccatgtgaa
    33901 acaccgttgc ccaagcccca acgccgttca acttatcagc ttgccctgtc atttcaagtt
    33961 caaagcatac ggagggactt ctcaatggcc acagctaacc cacatcctca agtggccggg
    34021 atcctctcgg ccctgctggc cggcgctggg caggcataca acggacagcg cacgaagggt
    34081 attgcctata tggcggtgga ggccgtcttt ctcgtgctcc ttttcaccgt actacgcgac
    34141 cctctctatg ggctcgtaac tctcaaccct gtaggaaggt tcgttgattc gaggcacatc
    34201 cttctggcag ggatcattgc agcattcgtt gttgctatct acgcatggtt tcacatagca
    34261 aatatcgtag acgctgtgag aaccgcatca tccatcagaa aacgccatgc gctccccgac
    34321 gacatgcagc gcatacaccc cgaataccgg acccccgctg gcacgatagc gccctatttg
    34381 tacatcgcgc cctcgctctt ggtggccttc ttcgtaatcg tgatacccct tgcgttcggg
    34441 attgcgctcg ctttcacaaa ttacaatctg taccattgcc ctcccgcagg cagattcgat
    34501 tggattggcc tcgagaattt tcgcagactg ctcaagcccg gatctctgtg gaaagaccag
    34561 ctcgacatgg tccttgtatg gaacatagtc tacgcgctat tgggcacggt tctcgcgttc
    34621 ctctgcgggc tagccctatc cctcatgctc gagaaccgac acatacgatt caaaggcgcc
    34681 tttcgcacca tactgatgct tccgtgggca gttcccgcca cagtatcgat catggtgttc
    34741 ttcggcctgt tcaatacgac gtttggcccg ataaacgaaa tcctgaaaaa catcgggctc
    34801 aaggccgtgc cgtggtttca agatcgcaca tgggctcggg tgagcgtggt gctgacccat
    34861 gtatggatat cgactccatt taacctgtcg gtgatatcag ccgcactcca aagcatcccg
    34921 gaggagattt acgaggcagc cacggtcgac ggagcatcac cttggcaatc gtttagcaag
    34981 ataaccttcc ccctgctcat gtcggtagtt gctccgatac tagttctcac actggcaggc
    35041 aactttaaca acttcggcat catatacctt ttgaccggcg gcgggcctgc agtggcggga
    35101 agcaaaggcg caggagccac cgacatactc atgacatggg tctacgatct tggtttccag
    35161 caactccaat ggtcgattgc gtccgcactc gcagtattgg tcttcatatt cgtagttgtc
    35221 ttcagcttga tcaatttcag actctccggc gtgctgcgcc agctcagaga ggaagaatga
    35281 ggggaggcga atcagatgtc cgcaaacaca gctacaggga agaacgcgag aaaggcaaag
    35341 cgcactgtgg gcacggttct gatatatgca gggctcatca tagcctgcat catagtgctc
    35401 tacccccttc tctgggttct gtcgtgctca ttcaaaccca cagccgggct tgtgggatca
    35461 agcttgatcc catctaatcc cacattggca aactacagga agatattcac cgaccccaac
    35521 gtcaacttca gccgctggtt tttcaacacg ctcaagatat ccgttgtcag cgcattgttg
    35581 acgctcgttc tgacaacacc tgccgcgtat gccttttcgc gcatgcgctt tccgggcaga
    35641 cgccaaaccc tgatagggtt catgatatgc caaatgtttc ctgggttcat ggcgattgtc
    35701 gccctctaca cccttctggg ctgggctggg ctaatagata cccatctggg attgataatc
    35761 ctttacgctg ggggtgcaat acccttctcc atctggctgc tcaaaggcta ttttgactca
    35821 gtacccgtgg aaattgagga gtctgcgatg atcgacggag ccacgaagtt tcaggcgttc
    35881 accagaattc tcttgccgtt ggccaaacca atcctctacg tcgtcggact catcaatttc
    35941 ctcggaccat acgctgagta tctgcttgca caggtggtga taacttcagg aagcaagtgg
    36001 acactggcaa tgggaatgag gagcctaacg gtatcgcaat tcgctacgga ctggccaata
    36061 ttctccgcag tgtcggtgtt gacggcagtc ccgatcgtca tcgtgttcct ctcggcagag
    36121 aaatacatcg tctcaggcct cacccgggga gcgataaagt aggcatgaca tagccgacgc
    36181 agtgtatcga atcagattga cctgcttccc ccaagcaggg acgcgaattg ttggctcaaa
    36241 tatctttgag atccaaaacc ataaaggagg aaaggtagaa tgaaggttaa gcgtgggttt
    36301 ctcgccgtcg tttgtggcgc gttgattcta tctctcgcca ccatcccgtc gatggcggca
    36361 gatccccttg tagtatggtg tgagccacaa aaaatgggaa ccatagagcc tcttgcagcc
    36421 cagtggagca aacagacggg cattcccgtg aaggttgaac ccgtaggcgt tctcgaaacc
    36481 gccaacaaag tgcagttggc cgggccgatg ggcaaagggc cagatgtgtt ctgctgtctt
    36541 tctggccagc tgggcatgtt agtggtcaca gggaccgtgg ccccagtcga tcccaaggtc
    36601 ttcgatctgt ccaacttcat gaaagtaggc gttgatgcag ccaccttcga tggcaaactg
    36661 tacggcgtgc cgtacgacat ttccagtgtg gcaatgatct acaacaaaaa gatctggcct
    36721 gaagcaccca aaacctttga cgagatgatc gccaggtccc gcgacttgca aaagaagggc
    36781 atgttcggaa tcttgtggcc cctggagaac ttctacttca cttatgcgat aatggcgggc
    36841 tatggcgggt atgtctttgc ccccactaaa tcaggatgga acacgaagga tatcggactt
    36901 gccaacccag gatctgtgaa agcgatcaag ctgctcaaga cgctcagaga cgaagggctc
    36961 atccctgtcg gcaccgacca cgtggttgct cctgccaagt tcagtgaggg aaaggcagcc
    37021 gccatcatcg acggctcctg ggctctgccc ggaatcaagc aggctggcat cgattatgga
    37081 gtggcgccca ttccgaggct cgacaatggc acgtatccgg cgccgtttgt gagcctcaag
    37141 tggtggcacg tgagctccta cagccagcac aaagcagagg ctgcgaagct cattgcttat
    37201 cttacaacga aggacgcaat gtacaagtcc ttcaaggccg ccgagggcat ccctccgcga
    37261 ctcgacgttc tcgaaatgcc cgatgtcaag gtgatgcctg aagtcgcagg ctatggcgcg
    37321 caggcgaact tcggatcgat cctgccggac ataccggaaa tgaatcctgt atgggtgccg
    37381 atgaacaacg caatcgagat cgccctgcgc ggagacaaga gtgtagaggc agcccttgct
    37441 gatgcagtaa agatcatcga gcagaacata gccgagctca agtaggagta aaagtcggcg
    37501 gccacgcgcc gcaagatgca gtataaagca actggttgct agagacatga ttcaatatga
    37561 gctgacgggg cccgcccggc gccagatcca gacagacgcc agagggcggg cccatctgcc
    37621 catttcgtct gaggagggat acgaacatga cccaccaatt tgcagtgccc ttttccgatc
    37681 ccaaacccga ttttgaagga ttccgacaag tgcttgaggg aaagcgggac gcaaaccgag
    37741 ttcactatgc tgaactcttc gccgaccgcg agatagttga ggcaatcctc gcggactaca
    37801 tggggctcac ccccgtcccc tccccagaag acgactttga agagtattgg cgccagcaga
    37861 tagagtattg gtataagatg gggtatgact acattcgtgt cgctggcggc ctagagatac
    37921 cacgggccaa gcaccgaata gccgaagaca ctgcagccct ttcgagagga gaacggggct
    37981 ggatcgaaca gggaatcggt gaaatagcct cctgggccga ctttgaggag tatccctggc
    38041 aaactgtgga ggacatggac ttttcggcct acgaattcgt tgcaaaaaac ttgcccgaag
    38101 ggatgaagat gttagtctgc ccctcgagcg gtgtattcga gatatcgtca gaatacatgc
    38161 tgggattcga aggcatgagc atgatgctgt atgaagatcc ggagctcgta gaagccgtgt
    38221 ttcttcgtgt cggacaaatg ctgttcgatt tctaccgcaa cgcagtcaca atagacaatg
    38281 tggctggcat cttccaagga gatgacctgg ggtttaagac ctcgacattc ctctccccca
    38341 cacatctgcg caagctcgtc ttcccctggc acaagcgcta tgccgagctg gcccacgaac
    38401 gcgggcaggt atactggctc cattcctgcg gaaacttgca gaacgtgata gacgacctca
    38461 tcgacgatgt aggtatagat gcaatacact ccttccaaga cgaaatcatg cctgttgccg
    38521 acttcaagga cgagtatggg caccgggtgg gggtcctcgg gggaatagac attgacaaac
    38581 tgtgccgttt ggacgaagca gacctcagaa gctacgtgcg aaccacgata gacaggtgca
    38641 tgcccagagg ttgggctctc ggctcaggca attccatagc caattatgtg cctgttcgca
    38701 gcttccttat aatgatggac gagggggcga aatggagagg ataaggcact tgagcgcgac
    38761 gccatcgggc agcagaaact gaagcaacac accttcttgg agatctgcga ttagctcgat
    38821 aactttatcg atttccacat catgtcgctt gctgcgggca tgcctttgtg ctacagttgt
    38881 gtaagcatat ttctcaccaa ataaacgagt ccggagagac tcgatttgag gaggcaaaca
    38941 agtgaatacc ggcaaaaaag tagtactctc actgcaacac actatcgcaa tgttcggagc
    39001 aaccgtccta gttccgtatc tgaccgggat caaccccagc accgccctgc tcaccgcggg
    39061 actcggaact ctcctgtttc acctgatcac aggtggccaa atccccatct tcctcgggtc
    39121 ttccttcgca ttcatcgtag tcatccagac tgtagcggag aaattcggca tggcctacgc
    39181 aatgggcggc atcgtggtcg ccggcggcgt gtacgtgctc gtcgcgatcc tcgtgaaact
    39241 gataggatat gatttcttca agttcctgtt cccccctgta gtcactggaa gcgtcatcac
    39301 cgtgatcggg ctgacactgg cccctacagc aatccaaatg gccagctcaa attggcccat
    39361 cgctctggcg gtatttgcaa tcgcggccat ctcgaccctt gcattcaagg gattcatgca
    39421 gatgcttccg atacttattg cggtcgtgtc aggctacgtc atctcgctca tcgcaggaat
    39481 agtcgacttc gccccgatcg ccgatgcagc atggtttggc gtgcccgagc tcacattccc
    39541 gaagtttagc tgggaagcca ttgcaatcat tgcacctgtg gcgatcgtaa ccctcatcga
    39601 acactggggc gacataaacg ctaacggtgc agttgtaggc aaagacttcg tcaaagaacc
    39661 cggcctatgg cgaaccattc tcggcgacgg tcttgcaacc atgctcgccg gtgctcttgg
    39721 cggaccggcg aacacgacat acagcgagaa cactgccact ctgagcctta ctggggtgta
    39781 cgatccccag atacttcgag gcgcagccgt cttcgccatc atcatcgcct tctgcggcaa
    39841 gctcggaggg atcattcagt ccatacccga gccagtgatg ggcggcataa gcattctgtt
    39901 gttcgggatg atcgcgtctg tgggaatctc caccatgttc tccggagccg acatgaactc
    39961 tacaaggaac cggataatcg ccgcagctgt gctggtattt ggcatatcca aagtagtcgt
    40021 tcccgtcggt ccgttcgagt tcgagggaat gagcctggct acaatgactg gtgctattct
    40081 caacctcgtc ctcccccaag acaggcccga cgagtagata gctgcaactt agacccaata
    40141 tgaccggagg catgtcattc gatatggttc ggacggcgca tgcaaatggc atcgcgccgt
    40201 ccccagatat tcatacctga aagaaagctg gcacagtaca ggcccgagca atgagtcacc
    40261 atgtctctgg ataactcgtg attagtttct gcgctatatc caaatcgacc aatctttgcg
    40321 aatggccgat ttgcccatca atgtcttcca tgaaaactac ccgcactcaa aacagaaccc
    40381 gtcgcccttg cagtcagcgc accttcggtt cctggtccgg gcataaccga gctagagcct
    40441 gaagtagtgg catacattag gcgcaagggt tttgggatgc agttgtcaac agcaacatgt
    40501 tgctttcgaa tattctaggc cagacaggga ttgacgaggt ctgatttctt tagagaaggc
    40561 gtgcagcttg cccgatctgc cataaactgt gccgtattcg ctaaaaacag gatggcagac
    40621 cattcgcttg cagtccattc cgctttcatg tacacttgtc catgaacacg ctcagctgga
    40681 caggaggagt aatgattgaa aagagtcatt gcgctcattc tcgcgctagc agcgctggtc
    40741 gccgtctcat cgccagcctt tggagctgtt accttggaag tcgtatggat gggatggcca
    40801 aagaatctcg tcgataagca gcttgaagat ttcaggcggg ccaatcccga cattagcctt
    40861 gatattcagc tggtcccgtt caaccagcta tttcagacgc tcgaagtccg ccttgcatct
    40921 tcccgcccgc cagatgtgtt catcgtcgac ggaccaatga ccgcatctta cgcagagcga
    40981 ggctacctaa tgccgctcga cagccatttg agctccaagg agctatcggc atggtttccc
    41041 gcatctgtcg aggcatgccg atacagggga aagctctaca ctcttcccta cgccacctct
    41101 tcagtggggc tatacttcaa caaagacatc ttccgcgagc ataatgtgaa gctcccccct
    41161 gaagatccag gccacaggct tacctgggag caagtagttg atatcgcccg aaggctgacc
    41221 atagacaccg atggcgacgg gcagcctgat atctggggac ttattatcga acaatgcgac
    41281 aggccatacc agatcgttcc actcgcagaa tcgaaggggg gccgcccgct ctcgccagat
    41341 gggaccatat ctactggcta cgtcaactct gcggccttcg tggaggctgc gtccttctac
    41401 tggaggctct ttaatgactg gaaagtgagc cctaaaggaa tccttcaggc ccccatcgcc
    41461 cgggagtatt tcggcaatgg caaagcagct atgatgctcg gggcagagtg gaatatagct
    41521 caactgcgca acttcccgaa ctacgaattt gggctctccc cccaccctta ttttgcagca
    41581 ggacgtgaag taactccaac aggcgggtgg catgtgggca tcagctccaa aacatcgaag
    41641 aaagacgcag ccctcagatt tgcaaaatac ataaccggtc acgatgcagt cgctacatgg
    41701 gcgaaactct tcggcaacgc gccagcccga cgcgacgtat accacagctt gaacgagatg
    41761 ttctctgatc ttatgtggca ggtgttcatc tatgagatgg aaaacaccgc gtctcccaga
    41821 cctgtaaccg cgggttatct cgagtacgaa tccgctttga aagaggcttt cagcgcaatc
    41881 cagtacgggg cagaccccgc ggccacgatg aacaatgtcg ccaagcagat agacagagag
    41941 ctgagaaaat atcgttgatc ggacggacct ggactcatga catatcgtga ttctaaagcc
    42001 gcatttggtt ttcttgcccc tgccctggtc ttcctctgtt ttttcagact atggccggcc
    42061 gcaaaatcgg tggctgagag cctccgaggc tggagcagcg caggggcaaa aacctttgtg
    42121 ggcctcgcca actacagata tctgtttcga gaagatcctg cattctgggc ttcagtgcga
    42181 gttacactca agtatgctgc aatcgcaagc cccattacca tcgcagcatc gctgggacta
    42241 gctttccttc tacgtggagg cgggaaacgt tcatcatttc tccggacact ctgctttctt
    42301 ccctcagcaa tatccctttc cgtggtagca attgtctggg gcctcatact cgacccctac
    42361 tacggactgg caaacagcat cctgggtaaa cttggcatag caccgcagcc atttttgtcc
    42421 tctcccgacc aagcgctggc atgcttgata tttatcactg tatggaggaa cgcaggctac
    42481 tggatgatgt tcttcctagc cggcatggag gccgttccgg cagaagtata tgaggcatct
    42541 gctttagacg gcgcttcatg catgcaacaa gcatggagga tcaccctccc gctcctggcg
    42601 cgaaccatgt cattcgtgct cgtggcaaat accacgtcta atttcgtcac atttgctccc
    42661 gttttcatcc taacccaagg cggaccaatt ggatccacca atgtgctgat gtacgaatct
    42721 tataagagcg ctttcgtcta tctcgataca gggcgggcaa tggcaatctc gtcactgacc
    42781 cttctggggg ttcttgccct ttccgccatg cagttaaagt tgacacgggc ccaatttgaa
    42841 cactgagcga ctcgcaagaa gcatcttggc ccattgagcc acaatgcccg aactaccggc
    42901 aacgcaaact tgacaattgc acagaggctt gtcaagaatt actcagcggc agcaaagggt
    42961 ttatgccata atcggagttg atttgaatgc gtagcagaaa aagcgataag cttgctggac
    43021 cacgtgccgg aaggaccatt tcttttaggc gcaacagaga tggcgtggac tttgtgcccc
    43081 aactgagcac aggaaaggca ttggcgtcaa tctccaaaga aatcctgatg ctggctgtag
    43141 ccatcatcta tcttgtgcct ctgtggtggg ttgtcgcatc atcattgagg ccggcaggtt
    43201 ctctttatca gtacgccacg ccgatctcat ggcgaacctt cttccctgat gagctcggca
    43261 ttgcgaactt cgtcaatatc tttgccgaac tcgacttcgg acaagccatg tcaaacacac
    43321 tacttgtatc caccataacc gttgcgctcg gcctcctcgt caactcaatg gccggattcg
    43381 cccttgccaa gtttgacttc ccatgcaaac ggttggtgtt cattgtcgtt ctgatcacgt
    43441 tcatggcacc gttcgacacg atcgtaatac cgctccatca ggtaatcagc gatctgggaa
    43501 tgataaacac ccgcgcagct ctgatactcc cagccgtagg caatggactt gtcatattca
    43561 tgttcaagca attctatcaa gagatacctt ccgaattgct agatgccgca cgcgctgacg
    43621 gagcatcatg gccgcgaatc ttctggcagg tcgtaatgcc aatctcgatg cctgctgtcg
    43681 ccactgccgc agtgatgatg ttcttgtttc aatggaattc actcttttgg ccgttgatcg
    43741 cggcattctc ccctaagtat cgaacagttc aggttggagt agccacgcaa atctctgtcg
    43801 atcagaccca ttgggcaaat ctctttgcgt cagctgtgat gggaagtctt ccgccaataa
    43861 tcctgttcct atcgctccaa aaacgctacc tgaagggcat ctccagtttg ggcaacgact
    43921 gagcagcccc caaactcgca tttcgcgtcc acatggcatg gcctggtcaa cggctttctg
    43981 aatacagtga ggaataagga gtcggggagc atcataaaag gaaagacgcg gccccaatcg
    44041 cagaccgcgc cttccccgct cagatggtta ttctgttttg ttcgaacatc gctgcccacg
    44101 tatattattg acatgggcat cagaatctat tcacagcttt gactgccgga ctatagactt
    44161 ttagattgcc aaacagatcc ccccataccg caagcccttt ctagattggc atgggtgcag
    44221 ccggtcgatt gttgttgttg ccggctccgc aggcgccggc ctccttctcg cccttcccgg
    44281 cattatctgg gctgacattg gcaaccccgg ctctccatta tttcaactct aatcctcatc
    44341 atggcctttg tatgaagctg ggatactcgc gattctgaca cgccaagaac cctggctatc
    44401 tcctttatcg tcatctcctc aaagtagtac agcttgatca caagaccctc tcgctcaggc
    44461 aggtggtcga tggcatcagc aagggcgtca tagagctcat ccttttccag gatcatctca
    44521 ggatccgggc tgccatcgtc agacacgaag tcaaggagcg tagccacgtt gtgttcatca
    44581 tcaacggcga taagctcgtc gagggatgaa tatgacgcca tgctgatctc ccacgtccac
    44641 ctatggaggg tatcaatatc taccttcagc ctgtccgcaa tctcctcatc agtcgcaggc
    44701 cgtcccgtaa cgctttcggc atcggccatg gcgcactcta tctgcctggc tttgtgacga
    44761 acagatcgtg gcgcccaatc catccttctc aggttgtcaa tgatgctccc ccgtatcctg
    44821 gtcatggcat aggtctcgaa cttgactcct cgggacatgt cgaatctctc tatcgcatct
    44881 atgagaccga gcactccata tcctatgaga tcatcgtagt ctacgcctgg tatggcggcc
    44941 tgaggcatgc gtgaaacaat gtgcttcacc agatggacgt acttcacgat aagctgctcc
    45001 cgcactgcgc tactattcgt cttaccgtat tcctgccaca gcgcgaagtc atctttcatg
    45061 ctgtatcact ccgggtcaat ggcttttctg ataagctctc tttgcctatc aaaaacaaac
    45121 cttactatcg ctgtctcttc tctatccgta atcccgatga agctcaaacc aacgctgacc
    45181 ccgggctgtt caggcctgtc tgattcctca attcttacaa tctcagcagg cactttgaaa
    45241 gggccgccgt ctggtccaat cggcaagatg aggttgagtg tctcaccaca tttcaaccat
    45301 gacggaggat ctgcaaggaa aatgcatgtc ccaccaccac ttaggtctac agctacaccg
    45361 ctctcccatt gaacctcacc attgtcgtcg cagtccggcg cgtactggac atcgagcaat
    45421 attgggaccc tcacgaactc ccgtcgctgc tcgcgcctga caaacaacgg ctccgatatc
    45481 tccagcagct ttactctgcg tcccgattcg cctattactt ctgagtggta atgatagatc
    45541 gcgcctctat aaggatatgc aacttcaatc tcgtcgcctt tgcggatcat agatcgccaa
    45601 tcatccgtat acggagcata tatccaatat cctgacttcg agaccccttc taccttggtg
    45661 gcaaatttgg gcgactcatc atagcccttg atccttactt ctattctcgc atttggtttg
    45721 agaggaggaa aatccatggc aaccccctca caatagatag tccggagctg taacgcctaa
    45781 caatctcttg atgaactgcc ccactccggc ctggccttcg ttcacttggg gcatgccccg
    45841 cagccgtcgc gcgattgatt ccatcgctgc cgaagcaggg ctgtacgggt aagccaacac
    45901 aaacggttga cgctgtctta tggcctgact gaccgaaggg tcaaccggaa tgtaccctag
    45961 cgcatcaaat gtgaaatcga gaaatctgtc tgataagaga ctgagctgtt cgacaacatt
    46021 ttcagcctct tttcgactgt ggcacatgtt gaccacaata cggacaatgg cattgggatt
    46081 ctctcgggat atcaccttga tcatcgcata agcgtctgtc atggccgttg gctcaggagc
    46141 tgtaatgacg atcgcctctt gggctgacag aacaaagtcg agcacagtcc tggacatgcc
    46201 ggccgcggta tcgatgatga gcaggtcgcg tccgcgcact gctctggaaa gcgactctat
    46261 gaggcgcttg cgatcttccg gcgccatgtc cgcgatgtca cgcagtcctg atgcgccagg
    46321 tattatctgc acatccctcg ggcccgttac aatgatgtct tccaatgaca atgttccctt
    46381 gatcacgtgg tgcagattgt ggcgtgcagc cagccccagc agaacatcga cattggcgag
    46441 cccaagatca gcatcgagta ttgtcacgcg catgccgaag tcggcgagga gcatggcaag
    46501 gttcacaacg aagctggttt ttccgacgcc gccttttcct gatgtaaccg ccacaactct
    46561 cgttgtatca tctgattccc cgcgcgcgac ttttcgcctg ctttcctttc cctgcactcg
    46621 gctggctgcc tctctcagtt cttcggcctg atccgacatc ttgtcaactc ctccgtgtac
    46681 ctctcacctg agctcacacc ccatgctcat acggttttcc gccaatggga agagatcatc
    46741 tctgcaatct ctgagcaggt ggcagggtgg atatcttctg gcacgctttg cccatcggca
    46801 aagtatgaaa agggcctgcc cgacctggca tagacattga aaatcgcccc cagtgtcgaa
    46861 ctctcatcca atttcgtaag gatcaccttg tcgaacccca cctgggcgaa ccggtccacg
    46921 agattgtcca gatcttgcct tcttgtcgtc gccgacaaga ccagatgaat ctcttcaggc
    46981 aaagcggcat ccatcatgct cttgagctcg tacatccgtg taggactatt tgggctcctg
    47041 cctgcagtgt ctatcagcac tagctccgca tcggagcgct tcaccaaagc catctccatg
    47101 tctctgggac tgtagacgac ttcccacgga accccgatga tctccgcata tgtctttatc
    47161 tgttctattg cagctatcct gtatgtatcc gcagttatca gagccacctt cgccttctca
    47221 accaacgcag cgcccgcggc tatcttcgct atggtcgtgg tctttccgac ccccgtaggc
    47281 cctacaaatg ccaccacctt ccggcggcct ctcactatct ctatagggcc cgatacatca
    47341 accatcttgg cgacttcgtc aaaagcccat ctcagaatct cgtcgctttc agcggggata
    47401 ttgccgcact ccgacgcaac tgcattggca agcctgaacg cggttttctc ggaaacatcc
    47461 tcctcgacaa gaaactgata aaccgcggcc atctccgggg gcaggcttga cactctggcc
    47521 ctcaccgctg actcccgtgc gatcacgttg agtgttgcat ccactccttg agattcagta
    47581 acaaagccga ttccctgccg atccggcgta aaatctcctc gccccggctg ccccgattct
    47641 tcagatacgc ctttgtccgg ctcgcgccgc ctggcgtctc cctcaggcaa aggctcgcca
    47701 tccgactgag ataaagcgct cactgaccgg atctccgctt cattctgctc agctctctcc
    47761 agcgcaaccg aaaacttagg cgcccagaca gcctcatcac catctatagc agccagcact
    47821 tcgaatgccg gcttccggtt catgccgaaa aaactgagca ccttggcaaa tcctcgagcc
    47881 ctacactgcc tagcatcgag tatcaccgcc tccggcccaa ggtcagctct taccttgtcc
    47941 agggcctctt tcatcgttga accctcatac ctcttcaccc gcattgccta ggctcaccat
    48001 cccaactgca tttaccttca cttccggcgc aatctcgtca tacgaaagga ccaccgtctg
    48061 cggcacagct ctctctatta gtcgcctgaa atggggacgc aggccgctcg acgtgaggac
    48121 gatcggctga agtccgcctg atgccatcct ctcgctttcg gcaacaaggc tcgagataag
    48181 cgcccggtat accttcggat ccggagctag gtaagtgccg tgctcagtaa cgtccagcga
    48241 atcgaagagc agctgctcta atgacgggtc caatgtaaca acgtacaatc cctcaggctg
    48301 tgcatactgg ctacatatgg accttgccat tctcgatctg acatactccg ttagggtatc
    48361 gatgtctcta tatgtccctg cggcatcgcc gagggcctcc aagatcgtca caaggtcgcg
    48421 aatggagatc ctttctctca ccaagttctg aagtactcgc tgcacctctc ccacggtcat
    48481 ctgtgccggt atcagctcat ctaccaccgc gggagccgtc ttcttcacat tgtcaatgag
    48541 gttctgcaca tcctgtctcg acagcagctc aggcgcattg gccttgatga tctcagaaag
    48601 gtgcgtcgct attaccgagg cagggtcaac aacggtatag cccagcatct ccgcctcggc
    48661 ctttctggca gccggtatcc atatcgccgg aagcccgaaa gcgggttccc gtgtgtgtgt
    48721 gccctctatc tcctctgttg cagtccccgg gttcatcgcc atgtagcaat cagagagcac
    48781 ctcacctctg gcaacctcta cgcctttaat cttgaccaca tattcatttg gcctagcttg
    48841 tatgctgtca cgtattctca cagacggaac cacgattcca agctccagcg caatctggcg
    48901 gcgcaccgct gccacccggt ctttgagatc gcctccctgg tccacatcta ccaggggtat
    48961 aagagcatag ccgaagttga gctccatggg ctcaactcgc agcaacgcca taacatcctc
    49021 cgcatcagtc ttgggcttcg gcggcacaac atccctcatt cgcgcagccg cctcttcctc
    49081 atggatctcc tttgtcaccc tataggcaag aaagcctgtt ccaaaagcca aaagaagaaa
    49141 cggaattttc ggcaacccgg gcattagcgc gagaatcaac aacaatatcg atgtgatggc
    49201 caacgccctt ggttgcctaa acatctgaga agtaaactcc tgcccgagat ctgactcaga
    49261 cgcagtccta gtaacgacga tccccatggc cgtggagatg agaagcgacg gtatctggct
    49321 cgccagtccg cctcctactg taagcagagc gtaacgctga aatgcttcac ctccagagag
    49381 cccaaaccga accattccta cgattatccc cccggcaatg ttgaccagga tgatgatgat
    49441 cccggctatg gcatcgcccc taacgaactt gctcgcacca tccatggcgc catagaaatc
    49501 agcctcctgc tgaatctttc tcctccgcat cctggcctct tcctcg
//
LOCUS       DAOSYL010000008        48296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_597_length_48296_cov_7.913124, whole genome shotgun sequence.
ACCESSION   DAOSYL010000008 DAOSYL010000000
VERSION     DAOSYL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 48296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48296
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_597_length_48296_cov_7.913124"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..341)
                     /locus_tag="PLU15_02810"
     CDS             complement(105..341)
                     /locus_tag="PLU15_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29884.1"
                     /translation="MTALLIAVIVCTCASHAALASEWDPPEITGSAEWQIDYLPGEQK
                     VAANGLSLKVNLDGYFIDSIGYNLDFGGRLDMLS"
     gene            complement(422..1348)
                     /locus_tag="PLU15_02815"
     CDS             complement(422..1348)
                     /locus_tag="PLU15_02815"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOO29885.1"
                     /translation="MTSQSFPGSGRSNVLSKHAARSSQRVDLSRLGICRGKVGALAGI
                     AIVLFAAVACAALLCAGAVAAAGLQVRGDLTAKQILDSIEGKGSLAGNGRAVVEMVVE
                     RGKSQKLNRVEVMRSDDGNGTIKQLVEFLAPADVRGMKFLSITAPGEEDQMWLYLPAV
                     GSERRIAGSAASGKFMGTDFTFEEISASAKTWEPYKPERMPDETVDGQKCYVLRLMPE
                     SGNLPYGAVTIWIWQENVLPLRIEFFNNSKKLQKVMTFSDLKKNKSGEWQPHTIVLQD
                     ILGGSLTTVRILEMDDRPVPDEVFTLRYLRKR"
     gene            complement(1376..3763)
                     /locus_tag="PLU15_02820"
     CDS             complement(1376..3763)
                     /locus_tag="PLU15_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO29886.1"
                     /translation="MSFIAGFVCRRPWVVIAAVIAITCISILGTMRIRVSSGLREFYA
                     QTDPQIKSMDLIEETFGGSEYIMIAFPSEQVFTVRGLRALSALSEGLEEIEGIARVRS
                     ITNIAEVTGTPWGLEVSQTLEDMPETEEEVEAFRVRIEESSEISGALLSGDGDHVLTL
                     VGLSPGADAGRVAKSVQEATGRLAPDFSPHYAGGPVLAGAADEFLKTDIKKLLPVCTI
                     VIVVVLFASFRTVAGVVLPLATVMLSVVWTMGLMGFADIPFTQITSALPVVLLATGSA
                     YGIHIMHRYYENHESGADGSSAASSAVESVGVAIVLAGITTIAGYGSNAASSVVRIRE
                     FGLLAGFGVSAGLVIALTFVPAVLSIFRPAPRKVALEGSGCPGGGCPPVVARIGRYGS
                     TVNDSSGCASKEHPVGAGEVERSWRDSLFAALSRLVARRGWATGLIAAALAVFALAGV
                     ARIQVDTSPVTFFPSDSVERRDFDLIRSEFGGVDTVQIMVGGDILDPKTLSAIERVHG
                     EFESVGGYGRALSIVNVLKQSSRALHDNDKAWERLPSTRKEAAQYVLLVSMSGDVGLD
                     EMITIDNRKARIEVMADTVDNSTNKASRLQSAVEIVKANLSGVSTISSLSITGMPFLE
                     QSMGEMVMSSQKMSLLFAAIAVAFIVYMSFGRSWDSFLCLLPVALTILANFGMMGWAK
                     ISLNLATALVSSIAVGIGIDYSVHVYSRFRQGIMEGYSPEDAMRATIANTGRAVALNA
                     LAVAAGFAVMLLSEFPPLQQFGALIAFTMAVSAGGALMILPASLLAVARRRSLAP"
     gene            4078..4347
                     /locus_tag="PLU15_02825"
     CDS             4078..4347
                     /locus_tag="PLU15_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29887.1"
                     /translation="MNQFTLHLDKEFLDAVERTVAEACEEMRRSSRRNSAGANWAFFA
                     KSAWDEAIEEAIGHVLDQRAGSVLGLMRPASQQVARSRDRTLNRL"
     gene            complement(4420..5250)
                     /locus_tag="PLU15_02830"
     CDS             complement(4420..5250)
                     /locus_tag="PLU15_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29888.1"
                     /translation="MKAARNIPMTVLVNFLLIVGLVGMAAPFVWMLSTSFKGTKDIFR
                     YPPQWIPQEPTLDNYRKLFRTVNIGRPLMNTVITAVSITGLSLIICSMAGYAFAKFEF
                     PGKNVLFMGVLGTLMVPGQITMIPVFLLLKKLGLLNTYSGLVLPGIASAFGIFFMRQF
                     MVTLPNELLEAARIDGAGEFFIFNRIVLPLCKPALATLGILNFTGSWNNFLWPLIIST
                     DESMYTLPVAVANLAGQYATEYGLQMAGSVMVIMPIVIFFLFAQQWFMEGVAMTGMKG
                     "
     gene            complement(5300..6181)
                     /locus_tag="PLU15_02835"
     CDS             complement(5300..6181)
                     /locus_tag="PLU15_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29889.1"
                     /translation="MQRNAKVAYMFLAPALVLMMVFVVIPVVASFVISLTNFDIFALA
                     DWSNAKFIGFDNYVRLFQDELFWKALSNTLYFVAVGVPLAIVLSLILAVLLNSPFNHA
                     KDLFRVGYYLPSITETVAIAVVWRWVLNPRYGILKRALEIFGLPSPNWLGDPRWAMPA
                     IIMMAVWKGLGHNALIFLAGLQSIPSTVYEAAQIDGANGWEQFRFITLPLLAPTTFFV
                     AIMTLIGYLQLFAEPYTLTDGGPLDATLSIVLYMYRHGFKFFNLGYASAMAYVLFAMI
                     FVATLLSTRSRAEEVTY"
     gene            complement(6381..7595)
                     /locus_tag="PLU15_02840"
     CDS             complement(6381..7595)
                     /locus_tag="PLU15_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO29890.1"
                     /translation="MRRAASMILCGALLLGIMVSSAAAAPVRLTVWAMGAEAQKIGEM
                     TAKFEQANPDIRVTVQAIPWSAAHDKIITAVAGGTGPDVAQMGTTWMAEFGSIGVFEE
                     LSRYMSKSKVVDKSQFFSGSYGTCVVGGKVYGVPWYVDTRALFYRKDLLAQKGFKQPP
                     QTWDELRAAAAALAADGKGYGMALSTNNYQEFLPFVWQNGGRILDEKGRPQVTEPEFV
                     EALDFYAKLFHDKIAPIDSGGSDLFQEFAKGTMPMYFSGPWMINLTREQTPEIDGKWS
                     VALCPKKKTRTSFVGGCNLVVFKDSRNKDAAWRFVEFMSDPRTQLEWFRVSGCLPAVK
                     KSWDARDLAGNPMMAVFREQLDDARAPENVPQWAQIEVAVQRRVQEACYGKKTAMEAA
                     RALEADIKAIMR"
     gene            complement(7649..10747)
                     /locus_tag="PLU15_02845"
     CDS             complement(7649..10747)
                     /locus_tag="PLU15_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOO29891.1"
                     /translation="MKWSESKGKRALNASICALALAVVFAFSCHWPANAAGIDRTYLF
                     GMDTEEEIAKFDNDNTGARLELTREVVRQGFGAMKVTPNGKALETKVAVDIDGVTLNE
                     WIGKDVLAVHVYIPEDMAIAPNSFFLGMADLSDGWTWVDGVFPKVEVVPGWNDVVYKL
                     SDGMKKVKEGGHYKLYFSFFAVDEGGAKIPLTESFFLDGIWLETLDGDASASSGAPVG
                     DWLWAMDDEAELAGYENDNTGAKFELDAGIVCQGLRSVAVIPDGKAIETKLALDLTGE
                     RLERWIGEHEVVLNAYLPPENEFNPTMFFLGMADLSNGWDWVGGIFAKVEPGPGWNRI
                     TYPLPDEMSNLKPDGKYKLYFAWAHVDEAGKKLPLREKFYIDGIGLAEARVSRDDLIQ
                     RIPEATRLEVAAMLELDDEELIEAVARKTFDYFWREANPANGLIRDRSTKDSPCSIAA
                     VGFGLSAIPVGIERGWITRDEGYERVLVTLRTFASGGVEGKNGFYYHFVDMSEGRRFG
                     DCEISSIDTAIFLAGALTAGQYFAGTEAEALANKVYAAADWEWMMNEGDTLSMGWKPE
                     IGFLSARWNSFNEGLLAYVLAIGSPTHPISASAWDNIFRPVNENYICLPQETLFVYQY
                     PAAWIDFRNKEDSYANYFNNAVTATRMNRLFAVLRRFNYSTYDMDIWGISACDGPAGY
                     KPYGASEGNHDGTVAPYASIASMPFTPELSMAAIRAMLEREGGLVWGEYGFVSGFNVD
                     QNWYSDQYVGIDQGIILLMLENYRSELIWKHFMAHPAIEEAMERIGFVKSDSDYAVTP
                     AYLDAWERMRLAPSEKKAVAKRAATPVVIDGDLSEWKGLEGYVVDEDMNVPAGGIEKV
                     DKKKQVLNSTFYVQYDEEYLYIAANVADEYIVINIKPEDKAGYYRTDSVEFYIDPSRA
                     GSGAGLMKLAILPFDTEGNVQAVRHEDTKPGPIAKTNPRIRVASSRTERGYAIEAAIP
                     LADLGIKAEPGTKLGFCHTVHNSNDREAKIGQYVRTNVIAWNNLPEIWANPDLWGELV
                     FE"
     gene            complement(10761..13163)
                     /locus_tag="PLU15_02850"
     CDS             complement(10761..13163)
                     /locus_tag="PLU15_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOO29892.1"
                     /translation="MRRFESKYGYFDETAREYVITTPHTPRPWVNVICPGDWGLVLSQ
                     TGGGYSWRTHASLNAITRWSQDIVRDNWGKYIYARDDNSGEIWSLSWMPARRKPDRYR
                     VRHGIGYSVIESENSGISSELTVTVPPGEPLEVWLVELSNDGSEDRELSLFTYFEWCL
                     GASPDWHREFHRTFIETSIEPDASTMWARKRLWEIPNDRGEAWNRPWEYSAFHASSLD
                     AAGFEGDKEAFLGMYGDLARPAAVERGALSNSHGKWTDAIGSIHVKIKLPAGEKRRVS
                     FLLGAVEAGREDDARAQIAQYRDPGEAADAMRRASEFWCDLLKPYEVRTPDEAFNIMN
                     NTWLPYQAISGRMWGRTGYYQAGGAYGFRDQLQDSQVFLPLDPSETAKQIKLHGAHQY
                     VDGHVDHWWHPISEIGGGGLYSDDLLWLPFVTIGYLKETGDYALLDEEVPYRDGEPES
                     IWQHCIRAIDLSLSRRSERGLPLMLGGDWNDGMNAVGTEGRGESVWVAEFLCTILPDI
                     AHVARRRGEEEAASRYEQAWRELADAVNSHGWSAEGGWYIRAICDDGTLIGDPGCKYG
                     KIFLNAQVWAIMSGIATGKRAESAWRAVVKHLSVDSGPVLFAPAYGEPDERIGYLTRY
                     APGVRENGGRYTHAATWAMIAAAIMGDSSEAWQMYSRICPVRCGSSPDVYKAEPYVTP
                     GNVAGPDSDRYCEGGWTWYTGSATWLFRVGSEWLLGVRPEWDGLRIDPCIPAHWDGFS
                     MRRNFRGATYEIHVKNPDHVSSGVFRMIMDGRKIDGCLLPIVGDGRVHRVEITLGKSN
                     KD"
     gene            complement(13219..14244)
                     /locus_tag="PLU15_02855"
     CDS             complement(13219..14244)
                     /locus_tag="PLU15_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO29893.1"
                     /translation="MSPTIRDIAEAAGVSTAAVSYVINDKPGVGEETRRRVLKIMREM
                     NYRPNPQARGLAARRTGMIGLMIPDITDPFYVNIVRGVEAKANELGYTLNLCTTHGDA
                     ERERDVAELFASGGVDGVIMMTYKLTPSELKELSEGRAPVVAIDNPEAAGWVPSVLVD
                     NIALGRTATSYLTELGHTRIGFIHGVEGSIASMRRYEGYVQALKSQGAEFSPELVEYG
                     GFTHAGGVEAARVLLSRPQRPTAIFAANDQMALGAMSAAMELGLSVPSDLSIIGVDDI
                     EAAALVVPGLTTLKQPTCEIGACAVGILADLIRSPEGDDEAGASDVGQRVFEARLIVR
                     GSCAPRQ"
     gene            14843..16468
                     /locus_tag="PLU15_02860"
     CDS             14843..16468
                     /locus_tag="PLU15_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29894.1"
                     /translation="MNMSNTRFNRIAKISIAVIALLTIPVRGFAESKQSSPGIVHARV
                     EADVTGDGINDAILLVGYQSELGGLFCRSHEVVVEDGATQKKATLDLGDLGAGYPGSL
                     SVGELDGAYGSEIVVAIPTGGSGGITNHFIVSARDGNVRMLVDAEELARGPKLDTRSL
                     DHFTIELVDKSKGDRFLLGLIKPDESDEFDPYSEYYSESGKLLQPIDICIDDVSAAEL
                     APIDGKSGLCEFVTYQKVWAIYHANSVGMIRTAWRYVDGELKIASIDVTPVLVPDAYT
                     EYLESIDHSDPADAVEKAISDYKLRFWNAPPAFRELAFAEFRKFHLAIAQAEAESLER
                     IAGIVDAKDSNDVLAAYARLPHIAADKNVSHAYKRAGLTTVYAGEGLWTVQPRAGFYA
                     EQFGTMLTPSVARFVELDDVERNEPWLVDSALVVSMKELSRRTAGWEAYLESNPYSLF
                     ADEARIRFNAAARALLLGTNNTPHHGSDGSIRPEAISALAYHAKAYPGTRSGKAAEDV
                     LQLVKKNANVWDNTVEKEIERVVEAAIYPDERL"
     gene            complement(16641..17969)
                     /locus_tag="PLU15_02865"
     CDS             complement(16641..17969)
                     /locus_tag="PLU15_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO29895.1"
                     /translation="MSNKLRACLLLLTVLFLSGLVMDVSSAGPTKIVFWYSVGGKVAE
                     TTRVLVDRFNATHPDVYVDAIYQGSYDDAMNKLKASIQGKSTPNVMQIYEIGSKFMID
                     SNAIVPVQKYIDKENLDVSSFERNILAYYEINDQLYSMPFNVSTPILYYNKNMFREAG
                     LDPGNPPVTFDDVARYAKILTVRDSAGRVSRPGMALAVYGWFFEQFLATSNLLYANNN
                     NGRDGVATAVAFANPQAASIIDWWVGMVREGVCANLGRKTADTQKAFISGQCAMTIDS
                     TAVLATILSGVDGKFEVGTCYLPRQAGAKGGVIIGGGSLWLLKATSAQEAASWEFIKW
                     MSSPEQQAFWHINTGYFPITKKAYDIPAVKQHQAKYPQFTTAVSQLRDSPITRATQGA
                     LLGVFTQAREIVEDAIEQVLAGRATSLEALKKAAQQIDAAIGRYNMTVGR"
     gene            complement(18104..18916)
                     /locus_tag="PLU15_02870"
     CDS             complement(18104..18916)
                     /locus_tag="PLU15_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29896.1"
                     /translation="MWQKAICYVFLTLAGVVVFFPIYYAIATSFFTPAESLSYPPTLF
                     PSKITLVSYEEALRRAPVFRFILNSFIVSASVMFGQLITASLAAYSFSFLEYKGRQVL
                     FYIVLSTMMVPWEVTIISNYLTIKALNWTDTYLGLSVPFMASAFGIFLLRQGFMTIPR
                     ELKDAAEIDGCSDLRFFTTIVLPLSRPMMATLAVYTFLNTYNQYLWPLLVTNVDRMRT
                     VQIGLAMLQWDESVAWNSTMAGVVMVLVPTAILMLFGHKQLVSGLTSGALKG"
     gene            complement(18961..19827)
                     /locus_tag="PLU15_02875"
     CDS             complement(18961..19827)
                     /locus_tag="PLU15_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29897.1"
                     /translation="MRKFVQGFLYLVPAIVVFVAFVFYPLANSIKLSLYQTDLFGKGQ
                     VFVGVQQYKDIFASGDFGRSLKITFLYALYTVIPGIVISLLLALMANWRLAGIRVYRM
                     IFSSPIVVAVASASIIWAMIFSPAQGMLRYILSLFGRPPVNWLADPKWALLSVSMVAV
                     WRGLGFNTILLLSGIQSVSEELYESAKLDGAGPWTMFWKMTLPMISPVLMFLLVVSTI
                     NALQSFGEINILTQGGPAGSTKVVVYSIYREAFFNYNFGFAAAEAMVLFLIVLILTII
                     QFGLMERQVFYQ"
     gene            complement(20394..21269)
                     /locus_tag="PLU15_02880"
     CDS             complement(20394..21269)
                     /locus_tag="PLU15_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO29898.1"
                     /translation="MKKESSRIHSFFRSGKGDSPAKKLLIHLILIMASVIAVYPFLRV
                     VAISLRPGSQLLSTDLSLIPSNATLENYRTLIFEKDFTIWLWNSLVVSLTTVIVGIVL
                     SASAAYAFSRWDFPGRKQGLVFLLTTQMLPPSMLLLPIYVMVVRLQLFNTYTGMILAH
                     SVGAVPFSIWLLKGYYDTIPRELEEAAFVDGASRLEAFYKIIIPLSTPALGVCGLFSF
                     MTSWSEFMMARVMLQKADLFTWPLGFQKLLDQFTTQWGVFSAASVLVAVPAMLLFMYS
                     SKWLISGLTLGGVKE"
     gene            complement(21266..22291)
                     /locus_tag="PLU15_02885"
     CDS             complement(21266..22291)
                     /locus_tag="PLU15_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO29899.1"
                     /translation="MIPLTWEIASRGLTEAGRILVMLAVGVAVIEALAYLILHKWLKL
                     KNALPFMLMLPAFIAVVALVIYPFLFNLQLAFSNMSMYRFKEFSVGLSYGIQNFLDVF
                     RAPVLQNVTFAQLLLRTVVWTAVNVFFHVLGGLFLAILLNRPLRGKAIYRTLLTIPWA
                     IPQVIAALAWKNEFHYQYGFINVLLQKLGFAPVQWLSDPKWAFVAVIIVNIWLGIPFM
                     MVIILGGLQSISPDYYDAAQIDGASGFQQFRAITMPLLKPVLTPAIILGVVWTFNNFN
                     VIYLITKGGPMEGTDILVTSLYKAVFEFYRYGFGAAYSTVILIILLIFTWIYLKVTKG
                     MGEVADA"
     gene            complement(22368..23630)
                     /locus_tag="PLU15_02890"
     CDS             complement(22368..23630)
                     /locus_tag="PLU15_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO29900.1"
                     /translation="MKRAIAFILTGVLFFALAVPMGALAQKKVTFTLWTKEGEVTGGV
                     LEDIVELSKEFAKSRPGLTIEVVSYGVEELRQNFQAAAFAGQGPDLLWTVSDHAGPFV
                     AMKIIKPVDNIFGKDFLKEFVRPGLECVELDGKTWGVPISVGNHLMLLYNKNLLKTPP
                     KNTDEMIKVGKELTRDLNKDGVPDQYGLVYNLNEPFFFAPWLGGFGGWPLDGTKPTLN
                     TPAMVNALQFVHDLKFVHKIVPVECDYDAADALFKEGKAAMLINGDWSLTSYQTPEVR
                     KKVDLGVARIPMVSQTGLWPTPMTSGIYFMLPDYLTGEKLQIAKEFVEFISSDKVQLR
                     FLEKHMRLPATASALKNPKIASDPLLKGSADQMVVGKPMPTVPEMRAAWDAIRPNQEA
                     VMAGKMSPKNAAKAMQDACEKLIREMTK"
     gene            complement(23826..25067)
                     /locus_tag="PLU15_02895"
     CDS             complement(23826..25067)
                     /locus_tag="PLU15_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO29901.1"
                     /translation="MISSDKCECNSNSESFEGAEAVAHRSGKSDTAQVPDGSRVAGRA
                     CDKRARRVTIREIAEAAGVSVSTVSHVLNEYGDISLDTERRVRQVMKEMNYYPSALAR
                     RLVKKRSYLFQLLIFSAEGLHHPFFYEVICGVTDEAEKAGYELLLSVKDSGDRRWRDS
                     LKRCYESKVEGLIVMGSLPGPELFEEIGVGGIPTVFIDIPYEGPRATYVSSDNVCGAR
                     MATEHLISLGHRNIAFLDGYDTGLPVQPLLHGGPAVDGCGTDSATPMWGISEARRAGY
                     AEALDAHDIIFDPDLILYGDYTQEGARRAVFQMLQERPDVTAIFAISDVMAIGAMQAV
                     RESGRKVARDVAVVGYDDIESASYVRPALSTVKQQGEDMGRSAVRQVLRLIGNPAEAP
                     AKVILPVELVVRESCGAGRGR"
     gene            complement(25680..26867)
                     /locus_tag="PLU15_02900"
     CDS             complement(25680..26867)
                     /locus_tag="PLU15_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29902.1"
                     /translation="MWTDFVNIWRKEMVTTVRDRKGMTQAILVPLIIAVFYASFTPLL
                     NKAMFERSQKPLIIPAKGVAYVDSELLDTMREFGITLEEYEGDLEAVIRRGDKESGLI
                     FEPGFAENVAEERPAQIRLVTNGTAGGIFGGGFSDSRLQLAVSVFNQKAAAQRVAVRG
                     IDPSALVPVVLETEDVATPEQEGALWASFYLPILVAVVLAQGGLFVAIDVTAGEKERG
                     TLDALLVTPASDWAILLGKMAAVFVIGLIPLLLSVTAFWVVSNMMPSVAAVSQQGLPV
                     FVLWGSLLIGIPMALVMSVAQMIVSVRARTFRDAQSAASPLVFAMMIPGFVAAFAPPK
                     ALWPCLIPLYGPFAALSRMAQAGKLQWDSLALATIGSLVLAVILMTVAVKLFDRERLL
                     YGA"
     gene            complement(26875..27717)
                     /locus_tag="PLU15_02905"
     CDS             complement(26875..27717)
                     /locus_tag="PLU15_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO29903.1"
                     /translation="MIEVRDLVKHYGDVRAVDGVNFVCSDGQITGLLGPNGAGKTTTL
                     RMICGLIRPTRGTVVVDGHDVIQDPASVRRVLGVQTDSSGVYPRLTPREHLRYYGAFY
                     GIAGDRLEQRIQEVIDALDMGEFADRRAEGFSRGQRQKVVLGRALVHDPKNIIMDEPT
                     TGLDVMAVRETRSMIRALRDKGHCVLFTTHYMDEAARLCDRIAVIIRGRIAAFGSPRQ
                     LMEQTGRDSLEDAFVELAGGGEGLLVSGDAGNGDNDDQTIVANGLDGCTEDSVRVGAA
                     VDRR"
     gene            complement(27911..29323)
                     /locus_tag="PLU15_02910"
     CDS             complement(27911..29323)
                     /locus_tag="PLU15_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HOO29904.1"
                     /translation="MRTKAKGVLLIVVVVALCAPRICSANMYPGSECHGTPDSSVDLN
                     NIEEIIRELASEKYMGRLPGTQGNWDAVEYVADYFRRLGLVCPESLDGYLQPYVQPVR
                     MFRRAPELQIVDAVGNLVSNFDYVEQYAPAPMQGWMVSKEVVGEGVVLERLSHLDDME
                     DGKILLIPLPISESAADMHILYNRLASEDEDPPEFAGIVLETDIAEIGYFPVYGLISQ
                     DVEFQDRDAHLSSGPVIFKCDVDVFSRLVEASGCGLRIRMDWDFAVEDVIAANVVAVL
                     LGPDGEVDEDHVIIGAHIDHMGSNFNGTYNPGAMDNASGMAAMMEIARLLAETAHRPR
                     RAVVFIAFNGEEEGMWGSRYYSNNPCYPLDEAVMINIDNVGSKYDVPLVIEEYAMMLT
                     SLVYHLYGIALQHGIDSEMGSGSMCDNASFALAGIEAVSLIHPDYSYGFHGPADTPDR
                     VDARRVAEIVDIILNYLYSY"
     gene            complement(29998..30789)
                     /locus_tag="PLU15_02915"
     CDS             complement(29998..30789)
                     /locus_tag="PLU15_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOO29905.1"
                     /translation="MAEEIRCLLVDLDGTIYLGDRLIDGALRLKDVAEEKGIQVFFMT
                     NNSSRSVNFYSEKLTRLGWPVGTDSILSSQEATALELSRRGVSSVYSVGTESLKAELE
                     RYGMTVHAGYDESAEYVVVGFDTTLTYEKLVIAGRHIQRGVRVIATHPDTVCPVGKTE
                     FIPDCGSIIACLKTAYEQMTVEVMGKPEAGMVKLVESRTPFRGKQIGVVGDRLYTDMK
                     FAKNWGMRSVLVLSGETSRDDITEENKPDIVVDHVGVLADMLLME"
     gene            complement(31054..32406)
                     /locus_tag="PLU15_02920"
     CDS             complement(31054..32406)
                     /locus_tag="PLU15_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO29906.1"
                     /translation="MVSVNGDSPSMPEGQKRSSRLINRTLMTFLVAMIFANIASEMGQ
                     PLMSLYVQSLGANVQQVGLLFTIASVVPLAFQILGGFVSDSIGRLQAIAIGSVAGIVG
                     YALHVVAPTWQWIILAQSISSLAGCFVAPSFRAFIAEQSSEEMRARCFALTDSIYMIV
                     GVVGPPIGGIVSEKYGYRAMFATAGVLYAVATIIRFAMAKSAQNRTGPVMERMTFPKL
                     KKDIAFMMSSVFAGGILTWLFISDGVFDVAYSTSYRFEPLYLGNIVGLSNRQIGLLSS
                     LFSGAMMILLPFGGWFADRFGERAGLVSGHALHAIGYLILLLFAREHWHFALIWILYG
                     IGAALLSPAYNSLISKVVPLELHGTAFGLFSTSLGLISLPAPYIGGLMWDNIGPKAPF
                     IVPMAASAIMIPVLWSKLDPGKAEDCGRGGIAENEGELSDSSGGDVSGDSSTPQDELS
                     "
     gene            complement(32749..33819)
                     /gene="thiL"
                     /locus_tag="PLU15_02925"
     CDS             complement(32749..33819)
                     /gene="thiL"
                     /locus_tag="PLU15_02925"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOO29907.1"
                     /translation="MEISKIGEFGLISRIDDGCVNDPSSVVQGIGDDAAVVRVSPGCM
                     LLICTDMLVEDVHFIRCAAPWTHIGYKAVAVNLSDIAAMGGEPKHCVVSIALPPSERV
                     ENVDRLYEGMKCLLAKDRVNLVGGDMVESSILAISVTMVGQADEKKIKYRYGARPGDV
                     VMVTGDLGASAAGLHLLEAELKSGTWTINSAPDMEEQPHQEDPRSTALRAHFLPVPRL
                     EESRTLVGLGPGCVTAMIDVSDGLASEVGHICDRSGTGALIYSDLVPVSESARMIADE
                     AGIDPLEWALFGGEDFELLFTVSKGCVRDVEDAFEAAGLCRVTAIGEITEPSLRRSIV
                     IAGGERVDLARDGYVHFRQAFR"
     gene            complement(34584..36251)
                     /locus_tag="PLU15_02930"
     CDS             complement(34584..36251)
                     /locus_tag="PLU15_02930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO29908.1"
                     /translation="MLYKEKYGVGEMKVQNDEKELLLKNGQILAIDEPNLQGDAILVR
                     NGRIAYVGAEEGAFSRCHGRPEVIDLEGRAVAPGFVESHMHPSILAGMMLDVDCTYPS
                     VKSVADIVDKIAERVDETPKGGWVRGWGYDDSKLEERVHPTRWDLDKVSPDNPVYIRR
                     TCVHMGVANSLALRLSGITADTPDPEGGRIDRDPATGEPTGLLMDRAQDLIAIPPYTD
                     QEVERGFALALGQLPKWGITTVHDMLVDADAFATYQRLAESHSLPARVRVWLEGITFG
                     SRDAGLIPFVLGSGLKSGYGNDMLKFMGVKFILDGSIGGCSAAVEEDYAGRKGEKGVL
                     YVERDELSPLVTECVKNGIRVSIHAIGERAIELAISSLEDAAREVSVESVRAMRNRID
                     HCVLPTRDHLKRIRDLGLVIEASTSFMYALGDSWISNLGYERACRTFPLRSAMDIGIR
                     VAGNSDSPVCDGSPLLGMYAAVTRKTSSGMTLGNSEKMTVDEVLRSYTAIGAYAGCEE
                     NLCGSISPGKFGDLVVLSENPRSVPPEHLKDIDVEMTFVGGVKVFAK"
     gene            36474..36725
                     /locus_tag="PLU15_02935"
     CDS             36474..36725
                     /locus_tag="PLU15_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29909.1"
                     /translation="MKRLSRLLALFLVVLLSLSLVGCGKKEEPEEDILDNDFEETETW
                     DYDEDDTESTDFDSDSWDFDWEDTTDEEDDDYDWGGSFE"
     gene            36928..37824
                     /locus_tag="PLU15_02940"
     CDS             36928..37824
                     /locus_tag="PLU15_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO29910.1"
                     /translation="MNCANGYANGNETLRLLTSRASCRDFSDKKIPQETINAVLNAGI
                     HAPTGGNLQPYSIIKVENHDMRIRLAEFCGDQGFIAKAPVNLVFCIDWHRLERWASLE
                     DAPFSATQSFRHFWISFQDTVICAQNICTAADAVGLGSCYIGTVLECLRELRELLELP
                     NGVLPVVLLAIGYPRERPMPRRKLGADVVVHDEVYRDLDAEELLRAYENKYEGFALEA
                     TEERVAILEEVCKAVHGDEFAVECLERVREAGLINAAQYRFGLHYRADKLPDGNEDLV
                     RATEEAGFNWFKRADMFVDDKD"
     gene            complement(38144..39148)
                     /locus_tag="PLU15_02945"
     CDS             complement(38144..39148)
                     /locus_tag="PLU15_02945"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOO29911.1"
                     /translation="MSKGSAEALLRVDNLTKHYPITQGIIRQKQVGAVRAVDGISFEV
                     RRGETLGLVGESGCGKSTTGRLLMRLEEPTEGSIRFDGCDWLSMGKEDLRRARKDMQI
                     VFQDPFSSLNPRMTVGEMVGEPLLVHGIAKGAELERRTAELLGVVGLDRSHAHRYPHE
                     FSGGQRQRIGVARALALNPKLIILDEPVSALDVSVQAQVINLLQDLQQEFGLTYIFIA
                     HDLAVVRHISNRVAVMYLGKIVELAQKDELCSSPLHPYTEALLSAVPIPDPKLRGGRQ
                     RIILEGDVPSPVDPPSGCRFHTRCRLAQDICSEKEPVLEDVGGGHMCACWVRGRTEVA
                     "
     gene            complement(39145..40086)
                     /locus_tag="PLU15_02950"
     CDS             complement(39145..40086)
                     /locus_tag="PLU15_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29912.1"
                     /translation="MTEDGEVTAVDDVSFDVWPGETLGIVGESGCGKSVTSLSVMRLV
                     QSPPGVIASGQVMFGGRDLLSIPEHDMRKIRGNEISMIFQEPMTSLNPVFTVGDQIME
                     AILLHQAVDKRTARKLAIEMLRKVGIPSPERRVDEYPHQMSGGMRQRVMIAMAIACNP
                     KLLIADEPTTALDVTIQAQILDLIAQLKSDSGASVMLITHDLGVIAETADRVVVMYAS
                     QIVETGDVMTIFEKPVHPYTAGLLRSVPKLNEETERLHPIDGVVPSPLRMPEGCRFAP
                     RCPNAQPACRREMPPLRRIAPGHEVRCIRAEKEADQK"
     gene            complement(40155..41573)
                     /locus_tag="PLU15_02955"
     CDS             complement(40155..41573)
                     /locus_tag="PLU15_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOO29913.1"
                     /translation="MKADLTIEERLAAVPEYTRFVTVDELYARAKAVAEARPDIAKLA
                     IVGKSTAGSEIPMVSIGDGPVSILIFACPHPNEPIGAMMTYFLMEELLKDEDLRAGRT
                     WHIMPCVDPDGTRMNEGWFAGPFNIRNYAHDFYRPRPQDQVEWTFPIEYKTLKWDTPK
                     PETQALMKAIETTRPDVMYSLHNAGFGGAYYYISSGLSKEDYDELHRIPIDRGLPLSL
                     GEPEVPWAVELYPGVYESCPITASYDYYEQFAPGDPAQYITAGAGSDEWAKTVCDPFS
                     IVTEVPYFVSAKIGDETPIGRTRREVVLEGIDRGRRIYELLAKDLTATEGLITRPEGK
                     LLRGAVETFVDSELKSIPSQERWARETSGMDQPATVAQEVDSIYVGVFYKMIVASMLA
                     RAIAGELGAESQEECCDGGYGCSCAPKRQILRSVLYDLNAHIDRWATDIEENLAYEVV
                     PIKDLIEVQYGALLVVLNAKGF"
     gene            complement(41659..42555)
                     /locus_tag="PLU15_02960"
     CDS             complement(41659..42555)
                     /locus_tag="PLU15_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29914.1"
                     /translation="MSKERERIEETEGTIRSEFAHVVQVFFRHKLGVLGLVMLVAFVA
                     IAIFAPVVAPCDPYEMDLANPFLPPGSPGHILGTDNFGRDMLSRLIYGSRISLLIGIV
                     VVSISSVLGSLLGMVAGYYGGWVDALVMRLVEVFYSFPFLILVIAVMAILGPSIFNVM
                     WVLGVVSWPLYARLVRAQVMALKSEDFIKAASALGCSDARIMFRHILPNVLTPVIVSA
                     TLGIPSAILSSASLGFLGFGVQPPTPEWGAMVSEAKDFMLINSNMIIWPGLCIFLVVL
                     SFNFVGDALRDALDPRLAKQLN"
     gene            complement(42548..43495)
                     /locus_tag="PLU15_02965"
     CDS             complement(42548..43495)
                     /locus_tag="PLU15_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29915.1"
                     /translation="MTRYIVRRLLATVPVIIGITFIVFAMVSFAPGDPIELMLGSETV
                     NPEYAERIRIELGLDRPWYEQYLRYMSGLLRGDMGRSIVYNRPVAGQVKERFANTAIL
                     AVSSAIFALLISIPLGVLAAAKRRTWIDYTATTLAMLGVSLPGFYLGLLLILLFGVRL
                     GWLPITGMGAAGSGAWEYIKHLILPSITLGSGMAAILTRLTRSSVLEALSQDFVRTAR
                     AKGVREKTVLYKHALRNALLPVITTFGLQFGSLLGGAVITETIFSWPGIGMLAVNAVK
                     QRDIPVIQGTTLVFALSFVLITLIVDIVYVFIDPRIRYE"
     gene            complement(43629..45209)
                     /locus_tag="PLU15_02970"
     CDS             complement(43629..45209)
                     /locus_tag="PLU15_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO29916.1"
                     /translation="MSKRTVLAIVAALLLVSASTVVLGAGPTKGGTLKIAASSIQQLD
                     PYKTAANDETNLCGLVFDPLVVIGKDSFEPKPHLAQSWEMPDENTWVFHIRKGVYFQE
                     GNEVFKKGAKREVTADDVVYSINRFINVSTAFTLGPIKSVKALDRYTVEIKTPEPSPF
                     LVSDPNRLPSVGIVPREAIEKLGEDGFARKPIGSGPFQLKSFSPDKGAVLERNPRYWL
                     PVYLDKVEFVVIPDPTVQVMAVTSGQVDIITYLVNMDAMPTISKNRKLAAVRGRGGSY
                     RGLGFNVTVSPFDEFAVRDAISKAMNIDAAFNAVVAPHGQRAYGQCPPWVAHGYDPSL
                     KSLWKYDPKEAVAILNKAGFRDTNGDGILDRNGQPLKVEIKTVPGSQVRVLTILATQL
                     RQIGIDASVVQQDTAVWVSDIMGGKSGVFFDFSFAGTTGLHSLFHSEAIGKTNGHFYA
                     DSTVDSLLDQALRTTDMKKLSSLWKQAQRKIMEDRASIPLYFEWGYSVVNKKVNDYVS
                     PWGGLHLVTLENNVWISK"
     gene            complement(45617..46354)
                     /locus_tag="PLU15_02975"
     CDS             complement(45617..46354)
                     /locus_tag="PLU15_02975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOO29917.1"
                     /translation="MLCHRLIALDLDGTVLTDDKRIADSTKLWVGKAADAGVIVTFAT
                     GRGRQDALSFVRELDLDSPMVFLNGAEIWGGPGQLLERHFMDTEDIYRLHQLTAETGC
                     CFWGYTVETLIQNDWSNDMLHQEWMKYGMYCDDVDLMAQLRDYVDSWGTVEVTCTSDA
                     NMEVTARGITKARGVRRICEQIGIEMEAVMAVGDGLNDLDLIEGAGLGVAMGNAAEEL
                     KRAADFVTESNEDDGVVLAIRRCIFGM"
     gene            46584..47489
                     /locus_tag="PLU15_02980"
     CDS             46584..47489
                     /locus_tag="PLU15_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO29918.1"
                     /translation="MTTSALILVLVSAVIHAGWNLATKKAEGGAAYVFLMAAGSALCW
                     FPLAAWLLMTGNVTTPGAGASPFSVVVAISVSGAIHTAYFLSLQAGYGCGDLSLVYPL
                     ARGTGPLLSTLAAMLLYGERPSGLALAGALLIGVGAFFLAGAEEPQATPSDDGSSKAI
                     VYGLLTGVLIAGYTMWDKYALDVVGVKPAFLSSAANCVRAIGMAPFFLLSKKRRAEVS
                     TAMRTQWKPALIVSISSPLSYLLVLTALATSPVSYVAPAREISILLGTLAGTRMLEEG
                     DPSSRKKRLWASVAMAAGVVALAIG"
     gene            47980..>48296
                     /locus_tag="PLU15_02985"
     CDS             47980..>48296
                     /locus_tag="PLU15_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29919.1"
                     /translation="MQIPWKSIVIVIFLVLAGLGFYASGPLARADTVTQVIRESVWIE
                     APNRFEIQGKNECTGFAVAYVLRHFEIEVTGKEIYDAVRFKLPSGRVLPRGPINELRR
                     HGI"
BASE COUNT        11598 a        14136 c        11617 g        10945 t
ORIGIN      
        1 taatcaagtc cgcagtttcc caactgacca cctatctgcc agcccgcata ttgaaaggcc
       61 ccggctgcgc accgatataa gcttggccca atgcagcttc cgccctacga gagcatatca
      121 agccgtccgc caaaatcaag gttatacccg atagaatcaa tgaagtagcc atcaaggttg
      181 actttgagac tgagtccgtt tgccgcaact ttttgttctc ctggcaaata gtcaatctgc
      241 cactcggcac tcccggttat ctccggcgga tcccattccg acgccaaagc cgcatgagag
      301 gcacacgtgc agacgatcac tgctatcaaa agagccgtca tcaacttcac cgcgccaaca
      361 gcaagtcaca ctctctcaac ctgtttcacc caacgcctca acagcccact ggtcacaagc
      421 ttcacctctt ccttagatat ctcaaagtaa atacctcatc cggcacaggc ctatcatcca
      481 tctcgagtat ccgaacggta gttaggctac ctcccaaaat gtcctgaagg actatggtat
      541 gcggctgcca ttctccgctc ttgttcttct tcagatcgga aaaggtcatc actttctgga
      601 gcttctttga attgttgaaa aactctatcc tcagcggcaa gacattctcc tgccagatcc
      661 agatcgttac agctccatac ggcaagtttc cgctttctgg catcaaccgt agcacatagc
      721 acttctgccc atccacagtc tcatccggca tccgctcggg cttgtaaggc tcccacgttt
      781 tcgcgcttgc agagatttcc tcaaaggtga agtccgttcc catgaatttg cctgatgcag
      841 cactgcctgc aattcgtctt tcgctgccca ccgcagggag gtaaagccac atctgatcct
      901 cttctccggg agccgtaatg ctcagaaact tcatccccct cacatcagcg ggcgctagga
      961 actccacaag ctgcttgatt gtgccgttgc catcatccga cctcatcact tctacccggt
     1021 tcaacttctg gctcttgccc ctctcaacaa ccatctccac cacggcgcgg ccattgccgg
     1081 caagagatcc cttcccttca atagagtcca ggatctgctt ggccgtaaga tctcctctaa
     1141 cctgcaagcc agccgctgcg acagcccccg cacacaatag agcagcgcac gctactgccg
     1201 caaaaagcac gattgcgatt ccggccaagg ctcccacctt gccccggcaa atgcccagcc
     1261 gggacaggtc aacccgctgc gaggaccgtg ccgcatgttt agagagaaca ttactcctgc
     1321 cacttccggg gaaactctgg gatgtcaaat caatgccctc ctttaaagaa cagacttacg
     1381 gtgctaagct gcgcctccgc gcaacagcta gaaggctggc gggcaagatc atcaaagctc
     1441 cgcccgcgga taccgccata gtaaaggcta tcaaagctcc gaactgctga aggggcggaa
     1501 actcggatag caacatgacc gcaaaacctg ctgcaactgc taacgcgttc agagcaacag
     1561 cccttcctgt attggctata gtagcgcgca tagcgtcctc aggactatac ccctccatga
     1621 tcccttgcct gaaacgggaa tagacgtgta cgctatagtc gattccaatt cccactgcaa
     1681 tagagctcac caaggcagtc gccaggttta gagagatttt agcccatccc atcattccga
     1741 aattcgccag aatggtaagg gccactggaa gcaggcaaag gaaggaatcc catgaccgcc
     1801 cgaagctcat gtagacgatg aacgcgacgg ctattgccgc aaacaacaaa ctcattttct
     1861 gactcgacat gaccatctcc cccatgctct gctcgagaaa tggcataccg gttatggaaa
     1921 ggctcgaaat agtcgacact cccgacagat tcgccttgac aatctccacc gccgattgga
     1981 gcctactcgc tttgttcgtc gagttatcta cagtatccgc catcacctca atcctggcct
     2041 tgcgattgtc gatggtgatc atttcatcta gccctacatc tcctgacatc gacaccaaca
     2101 gcacgtattg ggcagcctct tttcgtgtgg aaggcagcct ctcccaggcc ttgtcattgt
     2161 cgtgcaacgc tcgggatgac tgtttgagaa cgttcacgat tgacagagct cggccgtacc
     2221 cgcccaccga ctcaaactct ccatggacgc gctcgatcgc actcaaggtc ttgggatcca
     2281 ggatatcgcc gccaaccatt atctgtacag tgtcaacgcc gccgaattcc gatcgaatca
     2341 gatcgaagtc gcgcctttcg acgctgtcgg atgggaagaa tgtgactggg cttgtatcaa
     2401 cctggatgcg ggcaacacct gcaagggcaa aaaccgcaag tgctgcggca atcagcccgg
     2461 ttgcccaccc tcggcgtgcc accaaccggc tgagtgctgc gaacaatgag tccctccagg
     2521 accgctcaac ttctccagcc cccactggat gctctttgga agcgcaaccg ctgctatcgt
     2581 ttaccgtaga gccatatcgc cctattcggg cgaccacagg gggacagccg cctccagggc
     2641 acccactccc ctccagcgca accttgcgag gggcgggtcg aaagatggac aggactgccg
     2701 gaacgaaagt caatgctatc accaacccgg cgctcacgcc gaaccctgca agaagcccga
     2761 attcgcgaat cctcacaacg ctgctggcgg cattagagcc gtagcctgca atcgtggtaa
     2821 tgcctgccag cacaatagca acaccgaccg attccacagc agaactcgca gccgaactgc
     2881 cgtcggcgcc actttcgtgg ttttcgtagt atctgtgcat aatgtgtatt ccgtacgcgc
     2941 ttcctgtggc aagaaggacc accggaaggg ctgacgtgat ctgtgtgaac gggatgtcgg
     3001 caaaccccat gaggcccatg gtccatacaa cgctcagcat cacagtggca agcggcaaca
     3061 ccacccctgc cacagtgcgg aagctcgcaa agagtacgac gacgatcact attgtacaca
     3121 ctggaagaag cttctttatg tctgtcttga ggaattcgtc tgcagcaccg gcaagaaccg
     3181 gcccgccggc ataatgaggg gaaaagtccg gtgccaatct tccagtagcc tcctgcaccg
     3241 acttggcgac ccgaccggca tctgccccag gactgagccc gacaagggtg aggacatggt
     3301 ctccatctcc ggaaagcaag gcgcccgata tttcgctgct ctcttcaatt cgcaccctaa
     3361 aggcttccac ctcttcctcg gtttcaggca tgtcctcgag tgtctgcgag acttccaggc
     3421 cccacggcgt acctgtaacc tctgcgatgt tggtaatcga cctcaccctt gctattcctt
     3481 caatctcctc cagtccctcg gagagtgcgg acaaagcccg aaggcctctt acagtgaaca
     3541 cctgttcaga agggaaggcg atcatgatgt actcggatcc gccgaaagtc tcttctatca
     3601 ggtccatcga tttgatttgc gggtctgtct gcgcatagaa ctcacgtagt ccggaactga
     3661 ctctgatgcg cattgtacct aggattgata tgcacgttat ggcgattaca gcggcaataa
     3721 caacccacgg tcggcggcac acgaatcctg caatgaaact cattatctca ctccatccta
     3781 gtaacccctt tgtcagggtt ttgaagcatt gcgttggtta accgccctat cattatgact
     3841 gactagtcag tcataatgat agggttgttt cacctctctg tcaatacaca gatatgcacg
     3901 tgcagtcggc gcaattacca ttatccaaga aggacattca cagccgctga gagaattcta
     3961 atggagatga aacggggcgc agcgcagtcg ctggaagcct acggctccct gtagcccgtg
     4021 cccttggata atgcaaacga cgatttccat gctgcaacag agaaaggtgg aacatccgtg
     4081 aaccagttta cgctgcactt ggacaaggag tttctcgatg ccgtcgaacg caccgtggcg
     4141 gaagcctgcg aagagatgcg gagatcttcc cggaggaaca gcgccggggc gaactgggca
     4201 ttctttgcga agtctgcctg ggatgaagcg atcgaagaag ctattggcca tgttcttgac
     4261 caaagagcag gcagcgttct gggattgatg cgcccggcct ctcaacaagt tgctcgcagt
     4321 cgggatcgga cattgaaccg gctctgaaga taggggttga gcgtcgtgct tagcgtgaga
     4381 caattgcacc aagtcccgac tgctcaacct caaccagttc tagcccttca ttcccgtcat
     4441 cgcaacccct tccatgaacc actgctgcgc aaacaagaag aatatcacga taggcattat
     4501 caccatcaca gagcctgcca tctgcaatcc gtattcggtc gcgtattgcc ctgccagatt
     4561 ggcaacggca acaggcaatg tatacatgct ctcatcggtc gatatgatca acggccacag
     4621 gaagttgttc catgagccag tgaaattgag gattcccagg gtcgcaaggg ccggtttgca
     4681 cagaggcaat actatgcggt tgaatatgaa aaactccccc gccccgtcta tccgcgccgc
     4741 ctccaaaagc tcatttggca gcgtcaccat gaattgtctc atgaaaaata tcccgaaagc
     4801 gctggctatg ccgggcagaa caaggcccga ataggtgttt aggagcccta gtttcttcaa
     4861 gaggaggaag acaggtatca tggtgatctg ccctggaacc atcaacgttc cgagaacgcc
     4921 catgaaaagc acattcttcc cgggaaactc gaacttggca aaagcgtatc ccgccatcga
     4981 gcagataatc agagaaagtc cagtgattga tacggcagta atcaccgtat tcatcaaggg
     5041 tctgccgatg ttgaccgttc tgaacagctt tctgtaattg tcgagtgtgg gctcttgggg
     5101 tatccattgg ggagggtacc ggaagatgtc cttcgtccct ttgaacgacg tcgacaacat
     5161 ccacacgaaa ggcgcggcca tgcccacaag ccccacaatg agcaagaagt tgacgaggac
     5221 cgtcatggga atgtttcgtg ctgccttcat ccccgattcc ctccattcct gtcctttaac
     5281 gattcaaaca gctccctcat cagtaagtca cctcttcggc tcttgacctc gtcgatagca
     5341 gtgtggcaac gaatatcatg gcaaacagaa cgtaagccat ggcagaggca tatccaaggt
     5401 tgaagaactt aaacccgtgc ctgtacatgt aaagcactat ggacagggtt gcatccaatg
     5461 gcccaccgtc tgtaagggtg taaggttcgg caaagagctg caggtagccg attagagtca
     5521 ttatcgccac aaaaaacgtg gtaggtgcca atagcggcaa tgtgatgaac ctgaactgct
     5581 cccagccgtt tgccccatcg atctgcgctg cttcatatac cgttgacggg atgctctgaa
     5641 ggccggcgag aaatatcaga gcgttgtgac ccagtccttt ccacaccgcc atcatgatta
     5701 ttgcaggcat cgcccatcgt ggatccccga gccagttggg cgaaggcaac ccgaagatct
     5761 ccagggcacg tttgagaatt ccatacctcg gattgaggac ccatctccac accacggcga
     5821 tggccacagt ctcagtaatg ctaggaaggt aatatcccac cctaaacaag tccttcgcgt
     5881 ggttgaatgg agagttgaga aggactgcga gaataagcga caacactatt gcgaggggaa
     5941 ctcccactgc cacgaagtaa agggtgttgc tcaaagcctt ccaaaacagt tcgtcctgga
     6001 aaagccggac atagttgtcg aacccgatga acttagcatt gctccaatcg gccagcgcaa
     6061 agatgtcgaa attggtcagg cttatcacga aagaagcaac caccggtatt acgacaaaca
     6121 ccatcatcaa taccagagca ggcgccaaga acatgtatgc caccttagca tttcgctgca
     6181 tggcaacgac ctccaaacgc cgcacatgcc ttgccagcca gcaatcggct tcacgcagcg
     6241 cggctaataa cgaaaaccaa aaccggatcc aaaattcaag atcagggccg gatcgcctgc
     6301 gccgagaggt tcatgcccct cggcgcaggc tacgcgtggg tgagcgtggc ggcgagttcc
     6361 gcctcacttg cccacgtgat ctacctcatg atcgccttta tatccgcttc gagagcacgg
     6421 gcagcttcca tggcagtctt cttgccatag cacgcctcct gcaccctcct ttgtacagcg
     6481 acctctatct gggcccattg cggcacgttc tccggcgcac gggcgtcatc cagctgctcc
     6541 cggaagaccg ccatcatggg atttcccgct aggtctctag catcccacga ctttttcacc
     6601 gcaggaaggc atcctgaaac cctgaaccat tccagctgag tcctgggatc gctcataaac
     6661 tccacgaaac gccacgcggc atccttgttc cgcgagtctt tgaatactac aagattgcac
     6721 ccgcccacaa atgacgttct cgtcttcttt ttgggacata gcgctaccga ccacttgcca
     6781 tcaatctccg gcgtctgttc acgagtgaga ttgatcatcc atggtccgct gaaatacatt
     6841 ggcatagtgc ccttcgcgaa ctcctgaaaa agatcgctgc cgccgctgtc gatcggggcg
     6901 atcttgtcgt ggaagagctt agcgtagaag tccaacgctt ccacgaactc tggttccgtc
     6961 acctgcggtc ggcccttctc atccagaatc cgtccgccgt tctgccatac gaacggcaag
     7021 aactcttggt aattattggt tgaaagggcc atgccgtatc cctttccgtc cgcagccaga
     7081 gccgccgcgg ccgccctcag ttcgtcccag gtctgagggg gttgcttgaa gcctttctgc
     7141 gcaagcaagt ccttcctgta aaacagggcc ctagtatcta cgtaccaagg cacaccgtac
     7201 actttcccgc ccacaacgca cgtgccgtaa gatcctgaga agaattggct cttgtctact
     7261 acctttgatt tcgacatata ccgggacagc tcttcgaata ctcctatgga gccgaactcc
     7321 gccatccacg tggtgcccat ctgtgcaaca tctggcccgg ttccgcctgc caccgcagtg
     7381 atgatcttgt catgagctgc gctccacggt atggcctgaa ctgtcacgcg aatgtcggga
     7441 ttggcctgct caaacttggc ggtcatctcc ccgatctttt gagcctccgc acccattgcc
     7501 caaactgtca gtctgacggg ggctgcagca gcggatgaca ccatgatccc aaggagcaac
     7561 gcgccacaca gaatcattga tgccgctctt cgcatattca tgtccctcct tgatttatac
     7621 agtcgacgcc gctctcctca atctatcgct actcgaatac gagttctccc cacaggtccg
     7681 ggttggccca gatctcggga aggttgttcc acgcaatcac gttagttctc acatactgcc
     7741 ctatcttggc ttccctgtca ttactattgt gcactgtatg gcagaacccg agtttcgtcc
     7801 ctggttcagc ctttatcccc agatcagcaa ggggtatcgc cgcctctatt gcgtatcccc
     7861 gctccgtccg gctcgaggcc acacggatcc gggggttcgt tttggcgatt gggccgggct
     7921 tggtgtcctc atgccgcaca gcctgaacat tgccttccgt gtcaaaaggc aggatggcga
     7981 gtttcatcaa tccagcacca gatccagccc ttgaaggatc tatatagaac tcgactgagt
     8041 ctgtacggta gtagcccgcc ttatcctcgg gtttgatgtt gattacgatg tattcgtctg
     8101 ccacatttgc ggcaatgtac aggtattcct cgtcatactg cacatagaaa gtcgaattga
     8161 gcacctgctt cttcttgtcg accttctcta tgccaccggc gggaacattc atgtcttcat
     8221 ccaccacgta gccttcgagg cctttccatt ccgaaagatc cccgtctata acgactggcg
     8281 tggcggcccg cttcgccacc gccttctttt ccgaaggagc gaggcgcatc ctctcccatg
     8341 catcaaggta tgccggggta actgcgtaat ccgaatcgct tttcacgaat ccgattctct
     8401 ccatggcctc ttcgatggca ggatgggcca tgaagtgctt ccagatcaac tccgaacggt
     8461 agttctccag catcagcaag atgatccctt ggtctatccc cacatactgg tcggaatacc
     8521 agttctggtc cacgttgaat cccgacacga agccgtactc gccccacacc agcccgcctt
     8581 cacgttctag catggccctg attgcagcca tagaaagctc aggcgtaaac ggcatcgatg
     8641 cgatggacgc gtacggagcc acggtgccat cgtggtttcc ttcggatgca ccataaggct
     8701 tgtaccctgc aggtccgtca catgcggaga ttccccatat gtccatgtcg taagttgaat
     8761 agttaaacct gcgcaacacg gcaaacaagc ggttcatcct tgtggccgtc accgcattgt
     8821 tgaagtagtt tgcgtaactg tcctctttgt tgcggaaatc gatccatgca gcagggtatt
     8881 ggtagacgaa aagcgtctcc tgaggaagac agatgtagtt ttcgtttacc ggacggaaga
     8941 tgttatccca tgctgaagcc gatataggat gcgtgggaga gcctatcgcc agaacatagg
     9001 cgagtaagcc ttcgttgaac gagttccatc ttgccgagag gaacccgatc tccggtttcc
     9061 agcccatcga cagggtgtcg ccttcgttca tcatccactc ccagtccgcg gccgcgtaaa
     9121 ccttgttcgc caaagcttct gcctcggttc ctgcaaaata ctgtccggcg gtaagagcac
     9181 ccgcaagaaa gatcgccgtg tctatcgaag agatctcgca gtctccgaac cgtcgtcctt
     9241 cggacatgtc gacgaagtgg tagtagaatc cgttcttgcc ctcaacccct ccactggcga
     9301 acgttctcaa ggtgacgagc acgcgttcat atccttcatc cctggtgatc caacctcgct
     9361 ctatccccac tggaatcgcc gacaagccaa agcctacagc cgcaatgctg cacggcgaat
     9421 ccttggtgct tctgtcccga atcagcccgt tagccgggtt tgcctcgcgc cagaaataat
     9481 cgaaggtctt ccgcgctacc gcctctataa gctcttcatc atccagttct agcattgctg
     9541 ctacttccaa cctcgttgcc tcgggaatcc gctggataag gtcatctcgg gagactctgg
     9601 cctctgcgag ccctatgccg tctatgtaga acttctccct cagcggaagc ttcttccctg
     9661 cctcatctac gtgagcccag gcaaaataga gcttgtattt gccatcaggt ttgaggttgc
     9721 tcatttcatc gggaagagga taggttatgc gattccaacc cgggcccggt tcgaccttgg
     9781 caaatatgcc ccccacccaa tcccaaccat ttgagagatc cgccatgcca aggaagaaca
     9841 tggtgggatt gaattcgttc tctggaggca ggtaagcatt gagaaccact tcatgctcgc
     9901 caatccatct ctcaagcctc tctccggtca ggtcgagagc gagctttgtt tcgatagcct
     9961 ttccgtcagg aatgaccgca acagaacgga gtccctggca gactattcct gcatctagtt
    10021 cgaacttggc cccagtgtta tcgttctcgt atcctgccaa ctcagcctcg tcatccatag
    10081 cccaaagcca atcccccacg ggagctcccg acgaagcgga agcatcacca tcgagggtct
    10141 ccagccagat cccgtccaag aagaacgact ctgtcagcgg aatcttggcg cccccttcgt
    10201 cgacggcaaa gaaggagaaa tacagcttgt aatggccgcc ttccttgacc ttcttcatcc
    10261 cgtcagaaag cttgtagact acgtcattcc aacctggcac aacctccact ttgggaaaga
    10321 ctccgtccac ccacgtccat ccatccgaca gatccgccat gcccaagaag aaagaattgg
    10381 gcgcaatcgc catgtcctct gggatgtaaa cgtggaccgc cagcacatct ttgcctatcc
    10441 actcgttgag cgtcactccg tcgatatcga cagcgacctt agtctccaga gccttgccgt
    10501 tgggagtcac tttcatagcc ccaaaaccct gtctcacgac ttcccgagtg agctcgagcc
    10561 tggcaccggt attgtcatta tcgaactttg cgatctcctc ttcagtatcc attccaaaca
    10621 gatatgtccg atcgatcccg gctgcgttcg ccggccagtg acatgaaaag gcgaacacga
    10681 cagccaatgc aagagcgcag atcgacgcat tcaacgcgcg cttgccctta ctctcagacc
    10741 acttcatacg agccgcctcc ctaatcctta tttgactttc caagggtgat ctcgacacga
    10801 tgaaccctgc cgtcgcctac aatcggcaac aggcagccgt cgatcttgcg tccgtccatg
    10861 atcatcctaa agactccgga cgaaacgtga tcggggtttt tcacgtgaat ctcatacgta
    10921 gcgcccctga aattccgtct catggagaac ccgtcccaat gcgcgggaat acaagggtcg
    10981 attctgagcc catcccattc gggacggacg ccgaggagcc actctgagcc tacacggaac
    11041 agccatgttg ccgaaccagt gtaccaggtc catcccccct cgcagtacct atctgagtcc
    11101 ggcccggcaa cattgcctgg agtaacgtac ggttccgcct tgtacacatc tggcgacgat
    11161 ccgcacctca ccgggcagat cctgctatac atctgccatg cctccgatga atctcccata
    11221 atagccgcag ctatcatggc ccacgtggca gcatgggtat aacggccgcc gttctcccga
    11281 actccaggcg catacctggt caagtagcct attctctcgt ccggttcgcc gtaggctggc
    11341 gcaaaaagca cagggccgga atccactgaa aggtgtttga ccactgcccg ccaggcagac
    11401 tcggcgcgtt tgcctgtagc aataccactc attatcgccc agacctgggc attcaagaat
    11461 atcttcccgt atttgcaacc aggatcgcct atgagcgtgc cgtcatcgca tatggcgcgg
    11521 atgtaccatc ccccctcagc gctccagccg tggctgttaa ccgcgtcagc aagctcgcgc
    11581 catgcctgct cataacggct agccgcttcc tcttcaccgc gacggcgcgc aacatgcgca
    11641 atatccggaa gaatggtaca gagaaactcc gccacccaca cactctctcc cctgccctcg
    11701 gttcctacgg cattcattcc gtcattccag tcgccaccaa gcatgagcgg aagccctctc
    11761 tcgcttcggc gcgacaggct gaggtcgata gcgcggatac agtgttgcca aatactctca
    11821 ggctctccgt cgcggtatgg gacctcctca tcgagcaagg catagtcccc tgtctccttt
    11881 agatagccaa tagtaacaaa aggaagccaa agaaggtcgt cggaatagag gccgccgccg
    11941 cctatttcag agatcggatg ccaccaatgg tccacatggc cgtcaacata ctggtgcgcc
    12001 ccgtgcagct ttatctgttt tgccgtttca gaaggatcga gtgggaggaa aacctgactg
    12061 tcttggagct gatcacggaa tccataagcc ccacctgcct gataataccc ggtcctaccc
    12121 cacatccgcc cggagattgc ctgataggga agccaggtat tgttcatgat gttaaatgcc
    12181 tcgtccggcg tcctaacttc gtatggtttg agaagatcgc accaaaactc cgaggcgcgc
    12241 cgcatggcgt ctgccgcctc acccggatca cggtattgtg cgatctgagc gcgggcgtca
    12301 tcttcacggc ccgcctccac agcaccgagc aggaaggaca cacgcctctt ttcaccggcg
    12361 ggcagcttga ttttcacatg gatcgacccg attgcatcgg tccatttgcc atgtgaattg
    12421 gacaaagcgc cacgctccac cgctgcgggc cttgccaaat ccccatacat tccgagaaaa
    12481 gcctctttgt cgccttcgaa accagcagca tcgaggctcg atgcatggaa cgccgaatac
    12541 tcccacggcc tgttccaagc ctctccccta tcattgggga tctcccacag tctcttgcgt
    12601 gcccacatgg tgctcgcatc cggttcgata gaggtctcga tgaaggtcct gtgaaactca
    12661 cgatgccagt cgggcgatgc gcccaaacac cattcgaaat acgtgaaaag cgagagctca
    12721 cggtcttcac tgccgtcatt cgaaagttcg acaagccaca cttccagagg ctcgcctggc
    12781 ggcacggtga ccgtgagctc acttgaaata cccgaattct cggattctat gacagaataa
    12841 ccaattccat gccttacgcg atatcggtcg ggcttgcgcc ttgccggcat ccacgaaagg
    12901 gaccaaatct cgcctgagtt atcatcacgc gcgtatatgt actttcccca attgtcgcgc
    12961 acaatgtctt gagaccagcg ggtgatggcg ttgagcgacg catgcgtgcg ccagctgtaa
    13021 ccgccgccgg tttgggaaag gacaaggccc cagtctcccg gacatatcac gttcacccag
    13081 ggccttgggg tatggggagt cgttatcacg tattctcgcg ccgtttcatc gaagtagccg
    13141 tatttcgatt cgaatctcct catatgactt tcagccgcct ctcatcgctt ctgtatgtaa
    13201 aggttcccgc tgtcaatcct attgccgggg agcacatgat ccgcgaacaa tcaacctagc
    13261 ctcgaatacc cgttgaccaa cgtctgaagc gcctgcctcg tcatcgcctt cgggactacg
    13321 gatcagatcg gcaaggatcc cgacggcaca cgccccaatc tcgcacgtag gctgtttcaa
    13381 cgttgtgagg ccagggacca cgagagccgc cgcctcaata tcatcgaccc cgattatgga
    13441 tagatccgaa ggcaccgaaa gaccaagctc cattgcagcg ctcatcgctc cgagcgccat
    13501 ctgatcgttt gcggcaaaaa tggctgtagg ccgctgtgga cgggacagga gtacgcgcgc
    13561 tgcttcgact ccccctgcat gcgtgaaccc cccatattca accaattcag ggctaaactc
    13621 agctccctgc gacttcagcg cctgcacata gccttcataa cgccgcatag acgcaatcga
    13681 cccttcaacg ccatggatga atcctatccg tgtgtggcct agttctgtca gataggatgt
    13741 agcggtccgc cccagcgcaa tattgtcgac gagtactgat ggcacccacc ctgcagcctc
    13801 ggggttatcg atggccacta cgggggcccg cccctcgctt agctccttaa gctcagaggg
    13861 cgtgagcttg tatgtcatca tgataacccc gtccactcct ccgctggcaa agagctctgc
    13921 cacgtcacgt tcgcgttcgg catctccgtg ggtggtgcac agattcaagg tgtacccgag
    13981 ctcgttggct ttagcctcca cgccgcggac gatgttgaca tagaatgggt cggtaatgtc
    14041 ggggatcatg agcccaatca ttcccgtgcg tcgggccgcg agcccccttg cctgcgggtt
    14101 aggcctgtag ttcatctcgc gcattatctt gagcaccctg cgacgagtct cctcgcccac
    14161 accgggctta tcgttgatga cgtaagagac tgccgccgtc gatacgccag ctgcctcggc
    14221 aatgtcacgg atcgtgggag acataaccaa gccaccttcc aaaatcaaga ttgggtagcc
    14281 gaaccgcgaa aatcctcgca atcgtatctt caaggaacga ataattctcc acttaaccga
    14341 ttaagcacga gttccaatag aaggcgggca ttcattcccg ccgctcgccc cggctctgtg
    14401 ccagggacat cctaccaaat ccagccatcc tagcatactc gagcatgcag ttaaagcagc
    14461 ctcgtgttgt cttggacaac cttctgagga caacttaacc ggttaaggtt agaatagcaa
    14521 acatagagtt gcatgtcaat actggtcaag gaaaaccttg ctataggaat gggaggaaca
    14581 tgttgaaagg tcctgtcgac agatccgtgc aaatgctcta ttttagtgat tattgcttga
    14641 ctgggagtgt tcttagaatt cgagatcgcc tacacgccta aaccgcccaa gcccagccga
    14701 gatttaaaag cctcctccgc ccgttattgg gcaggttgct tttccataac ataccctgca
    14761 ttttgcatgc aattgtcgat ataatctaat tgcagcgtgc tttgcggccg cggcaaaatc
    14821 ttctatgtcg gagtgagaaa agatgaatat gagcaatacc cgattcaacc gaatcgcaaa
    14881 gatatccata gccgtaatcg ccctcctaac catacctgtg agaggattcg ccgagagcaa
    14941 acagtcatcg ccaggcatcg tccatgctcg tgttgaagca gatgtcacag gcgacggcat
    15001 aaatgacgcc attctccttg taggttatca gtctgaactt gggggtctgt tctgccgttc
    15061 gcatgaagtc gtagtagaag acggcgcaac ccaaaagaag gcaacccttg acctaggcga
    15121 tctgggcgcc gggtatcccg gttcgctctc agtgggcgag ctcgacgggg cctacggttc
    15181 cgagatcgtc gtagcaatcc caaccggcgg ttcgggaggg atcaccaatc acttcatcgt
    15241 atccgcgaga gacggtaatg tgcgtatgct cgtcgacgcg gaggagcttg cccgcgggcc
    15301 taagcttgat acccgcagtc tggaccactt tactatagaa cttgtcgaca aatccaaggg
    15361 cgatcgattt ctcctcggac tcatcaagcc agatgagtct gacgagttcg atccgtacag
    15421 tgagtattac tcagaatccg gaaaactgct tcagcctata gacatttgta ttgatgatgt
    15481 ctccgcagca gagctcgcgc cgatcgacgg caagagcgga ctgtgcgagt ttgtgacata
    15541 tcagaaggta tgggcaatct accatgccaa ctctgtaggg atgattcgca ccgcttggag
    15601 atatgtcgac ggcgagctca agatcgcctc aatcgacgtt actccggtcc tcgtgcccga
    15661 cgcatacact gagtacctcg aatccataga ccactcagat cctgctgacg ctgtggagaa
    15721 agccatttct gattacaagc tcaggttctg gaacgctcct ccggcattcc gagaactagc
    15781 cttcgcagaa tttaggaagt tccacttggc aatagcacaa gcagaagctg aatcactgga
    15841 gcgcatagcg ggaattgtcg atgcgaagga ctcaaacgac gtgctcgccg cctacgcccg
    15901 tcttccccac atcgccgcag acaagaacgt gtcacacgca tacaaacgtg ccggccttac
    15961 aacggtttac gctggcgaag ggctgtggac tgtacagccc cgtgccggat tctacgcaga
    16021 acagttcggg acgatgctaa ctccgtcggt cgcgagattc gtcgagctcg atgacgtgga
    16081 aaggaacgag ccatggcttg tcgacagcgc tctcgtagtc tccatgaagg agctgagtcg
    16141 tagaacagcc gggtgggagg catatctcga gagcaatcca tactcacttt ttgcagacga
    16201 agcaaggatc cgattcaacg cggctgcaag agcgttgctg ctgggaacga acaacactcc
    16261 ccatcatggc agcgacggat cgattcgccc cgaggcaata agcgcacttg cctaccacgc
    16321 aaaagcctat cctggcacgc ggtcaggaaa ggcggcagaa gacgtactgc agcttgtgaa
    16381 gaagaacgcc aatgtctggg acaacactgt cgaaaaggaa atcgagcgcg tcgttgaagc
    16441 cgcgatatac ccggacgaac ggctatagga accactgctc aatagccaaa acaaggcacg
    16501 attgagggca acccacagct gggttgccct tgcttttgcg tgcccgatcg aacgttttga
    16561 agtcgtctag agcacaaatt ggccagactc aaagcacgaa cactgttgca agaagaacca
    16621 tgtgccaagg cgcccagtga ctaccggcct acagtcatgt tgtatcgacc tatagctgca
    16681 tcaatctgct gggctgcttt cttcagagcc tcgagtgaag tggccctgcc agcaagaacc
    16741 tgctctatag cgtcctccac tatttcccga gcctgagtaa agacgccaag cagcgcaccc
    16801 tgtgtagccc tggttattgg gctatcacgc aactgactaa ccgccgtagt gaactgaggg
    16861 tatttggcct ggtgctgctt gaccgctggt atgtcgtatg ccttctttgt aatcgggaag
    16921 tacccagtgt tgatgtgcca aaatgcctgc tgttcggggg agctcatcca tttgatgaac
    16981 tcccatgatg ctgcctcttg cgcagaagta gcctttagaa gccaaagtga gccgccgcca
    17041 atgatcaccc cgccctttgc ccctgcttgc cttgggagat aacaagtgcc cacctcgaac
    17101 ttgccgtcta caccggatag tatcgtggcc agcacagctg tggagtcaat agtcatggca
    17161 cactgcccgg atataaaggc tttttgggta tcggctgtct tccttcccag attagcacat
    17221 acgccctccc gaaccattcc aacccaccaa tctataatcg aggcagcctg agggttggca
    17281 aatgccactg ctgtagcaac gccgtcccgg ccgttgttat tattggcgta gagtagattg
    17341 gatgtggcga ggaactgctc gaagaaccag ccgtaaactg caagcgccat tcctggccgg
    17401 gaaaccctgc ccgccgagtc acgcacggtt aagatcttgg cgtaccgagc cacatcatcg
    17461 aaggtaaccg gggggtttcc gggatccaaa ccagcttcac ggaacatatt cttgttatag
    17521 taaagaatag gagtcgatac attgaacggc atagagtaca gttggtcatt gatctcgtaa
    17581 taggctagta tattcctttc gaaggaagaa acatcgaggt tttctttgtc aatatatttc
    17641 tgaacaggca cgatcgcatt tgagtcgatc atgaacttgc tgccgatttc gtatatttgc
    17701 atgacattcg gagtagactt gccctgtatg ctcgctttca gtttgttcat ggcatcgtca
    17761 taactcccct gatatatcgc atccacatac acatcagggt gagtagcatt gaaccggtcc
    17821 accaacacac gcgtagtctc ggcgacctta ccgcctacgg aataccagaa gacgatcttt
    17881 gtaggacccg cggagctgac atccatcaca agcccggaga gaaacaacac ggtcaacagg
    17941 agtagacatg ctctcagctt attggacatc cttaatcctc ccccacatta ttcggtcggg
    18001 tcgaaaccac aggctgcaca ccctaacgtt tagccaatcc tccgccgcat atcaccgcct
    18061 tccccagaca tttcttctca catgcaggcc tcagattcgt catctatcct ttgagtgccc
    18121 cagacgtaag cccgcttacc agctgtttgt gaccgaatag cattaatatg gctgtaggaa
    18181 ccaggaccat cacgacgccc gccatcgtac tgttccaggc taccgactca tcccactgca
    18241 gcatggcaag gccaatctgg acagtgcgca ttctatcaac attggtaacc agaagcggcc
    18301 aaagatattg gttataggta ttaagaaatg tgtagacggc caatgtggcc atcatgggcc
    18361 tagacaacgg cagcacaatt gtggtaaaga accgcagatc gctgcatcca tcaatctctg
    18421 cagcatcttt caactctcgc ggtatcgtca tgaacccttg tctgaggagg aatatgccaa
    18481 atgccgatgc catgaacggc actgaaagac ccaggtaagt atcagtccag tttagtgcct
    18541 tgatagtcaa gtagttcgat attatcgtca cttcccaagg aaccatcatc gtggatagaa
    18601 ctatgtagaa aagaacctga cggcccttgt attccaagaa actgaaagag tatgcggcca
    18661 agcttgccgt aataagctgc ccaaacatga ctgaggccga tactatgaag ctattgagaa
    18721 tgaatctgaa tacaggggcc cgcctcaagg cctcctcata gctgaccagc gtaatcttgg
    18781 atgggaaaag cgtgggtgga tatgacaagc tctcagctgg agtgaaaaag ctcgttgcaa
    18841 tagcatagta aatcgggaaa aagaccacta ccccagcaag cgtaaggaaa acgtaacata
    18901 tggccttttg ccacatcgcc cttttcctgt gttgattggc agtaacgcgg cgcgtcgttg
    18961 tcattgatag aacacctgcc tttccatcag cccgaactgt atgatggtga ggatcaaaac
    19021 tatcaggaat aggaccatgg cctcagcagc tgcaaaaccg aagttgtagt tgaagaaggc
    19081 ctctcggtat atagagtaaa ccaccacttt ggtggaacct gccgggccac cctgggtcaa
    19141 tatgtttatc tccccgaacg actggagggc atttatggta gacacaacca acagaaacat
    19201 caagaccggg gagatcatcg gcaatgtcat cttccaaaac atcgtccatg gcccagcccc
    19261 atcaagcttt gcactctcat atagttcctc tgacacagac tgtatcccgc ttaggagtag
    19321 gattgtgttg aagcccaatc cgcgccagac tgccaccatc gaaactgata gcaaggccca
    19381 tttaggatct gcaagccaat tgacaggcgg gcgtccaaag aggctaagga tgtatctgag
    19441 catgccttgg gccggactga atatcattgc ccagattatg gaggccgaag ccaccgctac
    19501 aacaattgga gacgagaata tcattcgata gacccgaatg ccggcaagtc gccaatttgc
    19561 cattagcgcc aaaagcagcg aaatcacgat tcccggtatg acggtgtaca gggcatacag
    19621 gaaagtgatc ttgagactcc ggccaaaatc gccgctggca aagatgtcct tgtactgctg
    19681 cacacccacg aacacctgtc ctttaccaaa caaatccgtt tggtaaaggc tgagcttaat
    19741 gctgttggcc aacgggtaga agacgaaagc aacaaacaca acaatcgcag gaactaggta
    19801 aaggaacccc tgcacaaact tcctcaattc acggtgtttc ctgctagcac ggtccatctg
    19861 caataccgca aacgcccctt ccacatagga ttacacgtcc tgccttgctg cgcatgtttg
    19921 acgaaggcat cgaaaagagc aacatcacgc ttgctgcccg aaactacgtg tctggacatg
    19981 cagtacggaa gtagattcgc taccgccctt gttattcctt ttgtcctata tgctatttcc
    20041 attgtcacta tatagatgaa ggcgtctacc attaattcga taaaaaccta aagaaacggt
    20101 tcccacggag atgtggcact gaacatgcgt atgggtgtca tggcaaactt aaatgcagtc
    20161 aaggttgatc agatgtagtc tccatctgtg acccacgaca taaagcgcat gagacttccg
    20221 ccggtacttc ataaagtgca cccatgctca gttttaatac catggcagcc aggctgagct
    20281 tcatttgaag ctacacctgg ctgccatact gccccaatgg acattggagc tatcaaggtt
    20341 tttcgtcttc ccatctgtac tgcatcagta tccacgatga cccccagata tcgttactcc
    20401 ttcacccctc caagcgtgag ccctgatatg agccatttcg aggaatacat aaacagcaac
    20461 atagccggca cggctaccaa caccgaagcc gctgagaaga ctccccactg ggtagtgaac
    20521 tgatcaagaa gcttctggaa acccaatggc cacgtaaaca ggtcggcctt ctgcagcatg
    20581 actctggcca tcatgaattc gctccacgag gtcatgaaac tgaacagccc gcaaacaccc
    20641 aatgccggag tagatagagg gatgattatc ttgtagaagg cttcaaggcg actggcgccg
    20701 tcaacgaagg ccgcttcctc gagttccctg ggaatagtat cgtaatatcc cttgagcagc
    20761 cagatcgaaa acggcacagc gcccacagag tgcgcgagta tcattcccgt ataggtgttg
    20821 aacagctgaa gcctcacaac catcacatag ataggaagca ataacatgct cggggggagc
    20881 atctgcgttg tcaggagaaa gacaaggcct tgttttcgcc caggaaaatc ccatctcgaa
    20941 aaagcgtagg cggcactggc cgatagcacg atgccaacaa tcacagtggt aagcgagaca
    21001 acaaggctgt tccaaagcca gatcgtaaaa tccttctcaa atatgagtgt acggtagttc
    21061 tccaatgtcg cattggacgg tatcagcgaa agatcagtcg agagcaactg cgaccccggt
    21121 cgcaacgaaa tggccaccac tcgcagaaac ggatatacgg cgatcactga cgccattatc
    21181 aagattagat gaatcaagag tttctttgcg ggactgtctc ccttcccgct cctgaaaaag
    21241 ctgtgtatcc tcgaactttc ctttttcatg cgtccgcaac ctcccccatc cctttggtca
    21301 ccttcaggta tatccacgtg aagataagga ggatgatgag aatcacagtg gagtaggcag
    21361 ccccaaaacc atatctataa aactcgaaaa ctgccttgta aagcgaagtg accagaatat
    21421 cagttccctc cataggcccg cccttggtta tgagataaat gacgttgaaa ttattgaagg
    21481 tccaaactac cccgagaata atcgcaggcg tcaaaacggg tttgagcagc ggcatagtga
    21541 tcgccctgaa ttgctgaaac cctgatgctc catctatctg agcagcatca tagtagtccg
    21601 gcgatatact ctggagtccg cccagtatta tcaccatcat gaatgggatg cccaaccaga
    21661 tattgactat tatgactgca acaaaagccc atttgggatc cgacaaccac tgaactggag
    21721 cgaatcctaa tttctgtagc aagacattta taaacccgta ttgataatgg aattcgttct
    21781 tccaggcaag ggcggcaatg acctggggaa tcgcccaagg tattgtgagc aaagtgcggt
    21841 atatcgcctt gccgcggagc ggtctgttca gaaggatcgc cagaaagagc ccccctagaa
    21901 catggaagaa gacgttcacc gctgtccaca ccaccgtccg aaggagcagt tgggcaaagg
    21961 tcacattttg aagcaccggt gccctgaaca catcaagaaa attctggatg ccatacgaaa
    22021 ggccaaccga gaactccttg aatctgtaca tgctcatgtt gctgaacgcg agctgaagat
    22081 tgaataggaa tgggtatatg accagtgcaa ccacggcaat gaatgccggg agcatcagca
    22141 tgaaaggcaa cgcgttcttt agcttcagcc atttgtgcaa aatcagatac gcaagcgctt
    22201 caatgactgc gaccccgacg gccagcatca cgaggatcct tcccgcttct gtcagcccac
    22261 gggacgcaat ttcccaagtc aaagggatca cgtcaatcgc ctcctgggga aattggtccg
    22321 ggctgtgccg aaaaaacaac ccggaccgtt cgcttcatgg tttccgtcta tttcgtcatt
    22381 tctcgaatca acttctcgca agcatcctgc attgccttgg cagcgttctt aggcgacatc
    22441 ttgcccgcca tgaccgcttc ttgattaggc ctgatggcgt cccaagccgc cctcatttca
    22501 gggacggttg gcattggctt gccaacaacc atctgatccg cagagccttt gagaagcgga
    22561 tcagaggcta tcttcgggtt cttgagcgcc gacgcggtag ctgggagacg catgtgtttt
    22621 tcgaggaaac ggagttgaac cttgtcagat gagatgaact ccacaaactc ctttgcgatc
    22681 tggagtttct cgcccgtcag gtaatcgggg agcatgaagt atattcccga tgtcatgggc
    22741 gtcggccaga gccctgtctg gcttaccatt ggtatgcggg ccacaccaag atcaaccttt
    22801 ttcctaacct caggcgtctg gtaactcgtg agtgaccagt caccattgat caacatggcg
    22861 gccttgcctt ccttaaatag tgcatccgct gcatcatagt cgcattccac aggcacaatc
    22921 ttgtggacga atttcagatc gtgcacgaac tgaagagcat tgaccatcgc cggagtgttc
    22981 agcgtgggct ttgtgccatc gaggggccag cctccgaacc ctccaagcca tggagcaaag
    23041 aaaaacggct cgttcagatt gtaaacgagt ccgtactgat cgggcacgcc gtccttgttc
    23101 aggtcgcgcg tcagttcctt gccgaccttg atcatctcgt cggtattctt aggaggcgtt
    23161 ttaagcagat tcttgttgta cagaagcatc agatggttcc caacgctaat ggggactccc
    23221 cacgtcttgc cgtcaagctc cacgcattca agccctggcc ttacaaactc cttcaagaaa
    23281 tccttcccaa agatgttgtc gacaggtttg ataattttca tggcgacaaa tgggccagca
    23341 tggtccgaca cagtccaaag aagatccggc ccctgcccgg caaaggcagc agcctggaag
    23401 ttctgcctca attcctcgac gccataactg accacttcaa tggtgagccc gggacgggat
    23461 ttagcaaact cctttgacag ctcgacaata tcctccagca cccctcctgt cacttcacct
    23521 tccttggtcc aaagagtgaa cgtgaccttc ttctgagcca aagcgcccat cggcaccgct
    23581 agagcgaaga acaggacacc cgtcaatatg aatgcaatcg cccgcttcat cattggttac
    23641 ctccttccaa gttcgaccgt cgcacggtcg gagacaacat cggacccttc aaacctcgcc
    23701 gtactcgagt caacattaat cgctcacaga caacatagcc cataccaaac cggattcctc
    23761 aatctcgaga cttctgaagt cctttaccac ctcctgatcg acgagtagca gggccaacgc
    23821 caacttcacc ggccccgccc cgcaccacaa gactctctaa ccaccaactc gactggaaga
    23881 ataacctttg caggcgcctc agcagggtta cctatgaggc gcaaaacctg tcggacagca
    23941 ctcctcccca tatcctctcc ctgttgcttg acggtggaaa gtgcaggccg tacataggag
    24001 gcagactcta tgtcatcgta accgactact gccacgtccc tggcgacctt ccgccccgac
    24061 tcgcgcacag cttgcatggc gccaatagcc atcacatcgc taatagcaaa gattgccgtg
    24121 acatccggac gctcctgaag catctgaaac acggcacgtc tcgccccttc ctgagtatag
    24181 tcgccgtaaa gtatcaagtc cggatcgaag atgatgtcgt gggcatccaa agcctcagca
    24241 taccctgcgc ggcgtgcttc ggagatcccc cacattggcg tagcagaatc agttccgcat
    24301 ccatctacgg caggtcctcc atgcaatagc ggctgaacag gcaatcccgt gtcatatccg
    24361 tcgagaaaag ctatgttgcg atggcctaag gaaatgaggt gttcggtcgc catccgcgca
    24421 ccacatacat tgtcagagga tacataagta gcacggggcc cttcatacgg aatatcaatg
    24481 aatacggtgg ggattccgcc gaccccgatc tcctcgaaca gctcagggcc gggcagcgat
    24541 cccatgacga taagaccttc gaccttcgat tcatagcatc gcttgaggga atcgcgccat
    24601 cgcctgtcgc ccgaatcctt gacgctgagc aacagctcgt atcctgcctt ctcggcttcg
    24661 tccgttactc cgcatataac ttcatagaaa aagggatggt ggaggccttc tgctgagaaa
    24721 atgagcaatt ggaagaggta gcttcgtttc tttaccagtc gcctggcaag cgcactcgga
    24781 tagtaattca tctctttcat gacttgccga acacggcgtt ccgtatcgag gctaatatcc
    24841 ccatattcgt tcaagacatg ggatactgtg ctcacagata cgcctgcagc ttcagcaatc
    24901 tcccttatgg tgacacgtct tgcgcgtttg tcgcatgctc ggccggcaac acgtgagccg
    24961 tccgggacct gggccgtatc cgactttcca ctccgatgcg ctacagcctc tgcgccttca
    25021 aaactctcgc tattggaatt gcactcacat ttgtctgagc taatcatggc cgcctcccat
    25081 gctcccgaag gtctgtagta acattcttca agcaacgtgc gcaatacgac ggtcaaaccg
    25141 ggaacccaga cccgccgaac atacttgcgc gcaagtggcg ggtagtgcat gtgtgtataa
    25201 atggctggca tttgtattga taaatcgcta aaatagcatt tactataacg ttatagtcag
    25261 taagacaata gtacaatatg gttccgctta tgtcaataga accctgcgat aaaccagcat
    25321 gacataatct gcctagtttt gcgatcggga tgcaagcctt tgcataacac cgtttcctat
    25381 gggtaatgtt caagcagtgt aagaggatcg tgcaggatga ttcgaatgag gaaacaagcg
    25441 ggaagaagtt gtttggagcg atactcaggc gttcgggata gccggggacg aaatgcttat
    25501 ctgagtatac tttcgcaaac aaacaactac tcaactgatc atgaaggccg tttacatccc
    25561 cagtattgag catctcgtgg gaatactcta acggctgata tttgcctggc gcaggccaac
    25621 cggggaaagc cgaggctacg aagggctcga gaatgacgca acaccttatc aaaacttagc
    25681 taggccccgt aaagcagcct ttctctgtca aaaagcttga ccgccacggt catcaagatc
    25741 actgccaaga caaggctgcc aatagtcgca agcgcaagcg aatcccactg caatttgccg
    25801 gcttgcgcca tcctgctcag agcggcaaac gggccgtata gagggatcag acacggccat
    25861 agggcttttg gaggagcaaa agcagcgacg aatccgggaa tcatcatggc aaacacgagc
    25921 ggacttgcag ccgattgcgc atcacggaag gttcttgctc gaacgcttac tatcatctgt
    25981 gcaacactca ttaccaatgc catagggatg cctatgagca acgaacccca gagcacgaaa
    26041 acgggcaagc cttgctgcga tacagcggcc acagacggca tcatgttgct tacgacccag
    26101 aatgccgtca cgctgagcaa aagaggaatg agccctataa caaaaaccgc cgccatcttg
    26161 ccaaggagaa tcgcccaatc agatgcaggc gtgacaagca aagcatctag agttcccctc
    26221 tccttctcgc ccgcagtcac atctattgct acaaacaagc cgccctgggc caacaccacc
    26281 gccacaagta tcggaaggta gaaagaggcc caaagcgccc cctcctgctc cggagtcgca
    26341 acatcctctg tctccaaaac caccggcaca agagctgatg gatctatccc ccgaactgcc
    26401 acacgctgtg cagccgcctt ctggttgaaa acgctcactg ccagctgaag acggctatcg
    26461 gaaaacccac ctccaaagat gccgcctgcc gttccgtttg tcacaagtct gatctgggca
    26521 ggcctctctt cagctacatt ttcggcaaat cctggctcga atatcaatcc cgattccttg
    26581 tcgccgcggc gtatgaccgc ctcgagatct ccctcatact cctcgagtgt aatgccgaat
    26641 tcccgcatcg tgtccagaag ctcactatcc acatacgcaa ccccttttgc cggaataatc
    26701 aggggcttct ggctcctctc gaacatcgcc ttattcaaca gtggcgtaaa actggcatag
    26761 aagaccgcaa tgatcagcgg cacgagtatg gcctgggtca ttccctttcg gtcacgtact
    26821 gtagtaacca tctccttgcg ccaaatgttc acaaaatccg tccacatgct ctcctcacct
    26881 ccggtccacc gcagcaccga cccgaacaga atcctctgtg catccgtcaa ggccattagc
    26941 cacgatcgtt tgatcatcgt tgtcgccgtt tcccgcatct ccgctcacca gcagcccttc
    27001 gccgcccccg gcaagttcga cgaaggcgtc ttcgagcgag tcccggcctg tctgctccat
    27061 cagttggcgc gggctgccga acgcggctat cctgccgcgg atgattacag cgatcctgtc
    27121 acacaatctt gcggcttcat ccatgtaatg agtggtaaag agcacgcaat ggcccttgtc
    27181 tctaagagcc cgaatcatgc tacgggtctc ccgcacagcc atcacgtcaa ggccagtggt
    27241 gggctcatcc atgatgatgt ttttggggtc atgcacgagg gcacggccga gaactacctt
    27301 ttgcctctgc ccgcggctaa aaccctcggc cctcctgtca gcgaattctc ccatgtccaa
    27361 ggcgtcaatg acttcttgaa tcctctgctc cagcctatct ccggcaattc cgtagaacgc
    27421 accatagtac ctgagatgct ctctcggagt gagtcgcggg taaacgccgc tcgaatccgt
    27481 ttgcactcct aagaccctgc gaaccgacgc agggtcctgt atcacgtcat gtccgtcaac
    27541 gacaacagtg ccacgcgtcg gacgaatcag cccacatatc atcctaagcg tggtcgtctt
    27601 ccctgccccg ttcgggccca gcaatcctgt tatctggcca tcagaacaga cgaaattcac
    27661 gccgtcaaca gcccttacat ctccgtagtg cttgacgagg tccctcactt cgatcaaacg
    27721 attccaccct ttcgacgtcc gccttcctat ttattccatt taaaggtttt cccctacacg
    27781 attatggcgt gccaccgtct actgcataac aaagtatgaa caaggcacta acacaattgc
    27841 cagaatcata ccacgtatgt cggggtaagt ctattggaga gaactccatt gcagcagcaa
    27901 gttgccaaaa tcagtagctg tataaatagt tgagtatgat gtcgacgatc tcagcaactc
    27961 gtctagcatc aacacggtcc ggcgtatcag caggcccgtg gaagccgtaa ctgtagtcag
    28021 ggtgaatgag gcttacagcc tcaatgccgg caagcgcaaa cgaggcgttg tcgcacattg
    28081 aaccgcttcc catctctgag tcgattccgt gctgcagggc tatgccataa agatggtaga
    28141 caagactagt gagcatcatg gcgtattcct cgatgactag gggaacatcg tatttcgatc
    28201 cgacattgtc gatgtttatc atcactgctt catccagagg atagcacggg ttgttcgagt
    28261 aatacctcga tccccacatc ccctcttcct ccccgttgaa ggcaatgaag accaccgccc
    28321 gcctcgggcg atgcgcagtt tccgcaagca gcctggctat ttccatcata gccgccatcc
    28381 ctgaggcatt gtccatggcc cccgggttat acgtgccgtt gaagttgctg cccatatggt
    28441 cgatatgggc accgatgata acgtggtctt cgtccacctc tccatcaggc cccaaaagca
    28501 cagcaacgac attagcggca ataacatcct ccaccgcgaa atcccaatcc atcctgatgc
    28561 gcagcccaca tccgctcgct tcaaccagcc gcgagaagac gtcaacatcg cacttgaaga
    28621 tcacaggtcc cgacgagaga tgggcatcac ggtcctgaaa ctcgacgtcc tgcgatatca
    28681 gaccgtatac ggggaaataa ccgatctcag caatgtctgt ctccaggaca atgccggcaa
    28741 attccggcgg atcttcatct tctgatgcga ggcgattata tagaatgtgc atatctgcgg
    28801 cgctctccga tatgggcagc ggaatgagca ggatcttccc gtcttccata tcatcaagat
    28861 ggctcagtct ctcgaggaca actccttccc ccacaacctc cttagatacc atccaacctt
    28921 gcataggcgc tggggcgtat tgttctacat aatcaaagtt cgataccaga ttgccaactg
    28981 cgtctacgat ctgaagctcc ggagcacgcc gaaacatccg gaccggctgg acatatggct
    29041 gcagataacc atcgaggctt tcagggcaaa caagcccgag tcggcggaaa tagtcggcta
    29101 cgtattctac tgcatcccaa ttcccttggg taccgggaag acgacccata tacttttcag
    29161 aagcgagttc tcggatgatc tcctctatgt tgtttaaatc tacgctggaa tcaggcgtac
    29221 catggcattc agaaccaggg tacatattgg ctgagcaaat tcggggcgca cagagcgcca
    29281 cgactacaac gataagcagc acccctttgg ctttggttcg cacggatagt caccccctcc
    29341 gataagccag actttttgct gcgacgaaac aagaccttac gacctgtatt aattgccact
    29401 tcttctttca ttgtccagat ccttctcagt aagtagagtc agttgcagtc tgccgatgga
    29461 tcgctaaagc tctcaaacgt tcgcacgggt atctataata taccagcttg gcaagatcct
    29521 tgcgctgcct ccatcccatg agaccgctga ctcgttgtaa gggacgccct gaagcatgtc
    29581 acgaagggct ctgattggct catagtggat caggaaaagc acttgtcttg gagatggcat
    29641 atcgcagacc ccgcgctact tgggtgtggt accgtggagc ggccctacag ctattacact
    29701 cttcgtacaa agaaccccgc tcgccgagtt gccagcatcc tatttcatcc cgacatgaca
    29761 gcacacgccc gattaggccg caagggatct ggtaaccaca gcagattcag agcatgtgca
    29821 cagtcccgtc tgcgaaggga ccgcagcatc cggccttgcg gctttgcctg gattgatctt
    29881 gagcctgtaa acacgcaacg cctggtagaa tacgagcttt gaaggatgta aatggcgcaa
    29941 agcaaatcgc aactgaaaac cggggggcgg atcgtatccg cccctccttc cctttggtta
    30001 ttccatcaat agcatgtccg ccaatacgcc tacgtggtcc acgactatgt cgggcttgtt
    30061 ctcttcggtg atgtcatctc ggctagtttc ccccgaaagc actagcactg agcgcatgcc
    30121 ccagtttttg gcgaacttca tatctgtgta aagcctatcg ccaacaaccc ctatctgttt
    30181 gccccggaac ggggtccgcg actcgaccaa cttcaccatt ccagcttcag gcttccccat
    30241 gacctccacc gtcatctgct cgtaggcggt tttgaggcaa gcgattatcg acccgcaatc
    30301 cggtatgaac tcggtttttc ctaccgggca cacagtgtcg ggatgcgtag caatgacacg
    30361 caccccccgt tgtatgtgcc tgccggcaat cactagtttc tcgtatgtaa gcgtagtatc
    30421 gaaccccacc acaacgtact cagcagattc atcatagcct gcatgaactg tcataccata
    30481 gcgttccaac tctgccttga ggctttcggt gcccaccgaa tacacgctcg acactcctcg
    30541 ccggctcaat tccaaggctg ttgcctcctg cgagctgaga atcgaatctg tgcctacggg
    30601 ccagcctagc ctagtcagct tttccgagta gaaattcaca gaccttgaag agttgttcgt
    30661 cataaagaat acttgtatgc ccttttcctc cgccacatct ttcaaccgca aagcgccgtc
    30721 aatgagtctg tccccaaggt aaatcgtccc gtcaaggtcg accaaaaggc acctgatctc
    30781 ttccgccact caggatatac accccttaca caattgatct cagcatccca tcgatgtccc
    30841 gtattccaga atttgagcgc cataatgaaa aagcctgcca tcttcggcac caaacgacga
    30901 tcaggccgta tgcgccaaca tgacagacct ctcctgctct cccggtccgc gccattatcc
    30961 tttttaaaat aggcatgatt cggcctaaat caccaacatg tttgtctttt tctatacact
    31021 gcccgcaact cctctattgc tgccctaaag gaatcaactc agttcgtcct gaggagtaga
    31081 cgaatcccct gaaacgtctc cacctgaaga atcagaaagt tccccctcat tctccgcaat
    31141 tccacctcgg ccacaatcct cagctttgcc cggatcaagc ttactccaca gaaccggaat
    31201 cattatcgcg ctcgcagcca tggggacgat aaatggcgcc tttggaccga tgttatccca
    31261 catgaggcct cctatgtatg gcgcaggaag cgatatcagt cccaggctcg ttgaaaacaa
    31321 gccaaatgcg gttccgtgca gttcgagtgg gacaactttt gaaatcaagg agttgtaggc
    31381 ggggctgagc agcgcagccc cgattccgta cagaatccag ataagcgcga aatgccaatg
    31441 ttctctggca aacagcagca gaatcagata ccctatcgcg tgtaaggcat gcccagatac
    31501 caatcctgct cgctcgccga atctgtccgc aaaccatccg ccaaaaggca acaatatcat
    31561 catcgcccca gagaacaacg agctcaagag tccgatctgc ctgttgctca gacccactat
    31621 gtttccgagg tagagcggct caaacctata tgacgtgctg taggccacat cgaagacccc
    31681 atcagagatg aagagccacg tgagtatgcc tccagcgaat acagacgaca tcataaacgc
    31741 gatgtctttc ttgagtttcg gaaaggtcat cctctccatc acaggacctg tcctgttttg
    31801 agcggatttg gccatggcaa accgtatgat tgtcgcgact gcgtaaagca ctcctgccgt
    31861 tgcgaacatc gcgcggtacc cgtacttctc cgacacaatg cccccgatag gcggtccaac
    31921 aacccctact atcatgtata tcgagtccgt aagagcgaaa caccttgcac gcatctcctc
    31981 tgacgattgc tcggcgataa acgctcgaaa gcttggggcc acaaaacagc ctgcaagaga
    32041 agatatgctc tgcgccagga taatccactg ccacgttggc gccactacgt gaagcgcata
    32101 ccctacaatc cctgcaacgc tgccaattgc aatcgcctga agacgcccaa ttgaatctga
    32161 cacaaatccg ccgagaatct gaaatgccaa tggcacgaca gatgcaatcg taaacaagag
    32221 ccccacctgc tgcacgttgg cgccaagcga ctgcacatac agcgacatga ggggctgccc
    32281 catctcagat gcaatatttg cgaaaatcat ggctacgaga aatgtcatca gggttcgatt
    32341 aatcagacga gacgagcgct tctgcccttc gggcatcgat ggggaatccc cattaactga
    32401 aaccaattga ttatcccccg acgatctagc acttgagaga ggatcactgg ccgcgcagac
    32461 gcccaaatgg catcttcgcg tgatcacatg cccatagtat catcacttaa gccagtttgt
    32521 cgatgtttgc cagtctgcgc ctgatggcga accaattccc tgctgcacca tttctccgtc
    32581 atttcacaga cgcatctcgg ctctgccggg cagaaaaata gcacgaggcc atcctcgtac
    32641 agcccaatga ctcgttcagc gtcgtgctct cccctatctg cgctgattcg gatgatgtct
    32701 aaaacacccc gagacaagcc gacctcagta caatgctcca cgacaaattt atctaaaagc
    32761 ctgcctaaaa tggacgtacc cgtcccgggc caagtccacc ctctcgccgc ctgcaataac
    32821 aatggaccgt ctcagagaag gctcagttat ttcgccgata gcggtaacac ggcaaaggcc
    32881 agctgcctcg aatgcatctt ctacatctcg tacacaaccc ttggagacgg tgaaaaggag
    32941 ttcgaaatcc tcgcctccaa aaagagccca ctcgagtgga tcaattcctg cctcgtccgc
    33001 aatcatcctg gcggactcgg atactggcac gagatcagaa tagatcaagg cgccggtccc
    33061 actccgatcg catatgtgcc ccacttcgct ggccagtccg tcactgacat cgatcatagc
    33121 cgtgacgcaa ccagggccaa ggcctactag ggttctcgat tcttccagcc tcggaaccgg
    33181 caggaaatga gcgcgcaaag cagttgaacg ggggtcttct tggtgaggct gctcttccat
    33241 gtccggcgcc gaattgatag tccatgttcc gcttttcagc tcggcctcga gaagatgcag
    33301 gcccgctgct gacgcgccca ggtccccggt gaccatcact acatcgccgg gccttgcgcc
    33361 atagcggtac tttatcttct tctcatctgc ctggcccacc atggtcacgc ttatcgcaag
    33421 gatgctggat tcaaccatgt cgcccccgac gagattgact cggtccttcg caagcaggca
    33481 cttcattccc tcatatagcc ggtccacatt ctccaccctc tccgatggcg gaagagcgat
    33541 cgacactacg caatgcttag gctcgccgcc catggcggca atatcgctca ggtttacagc
    33601 cacagccttg tatcctatat gtgtccaggg agcagcgcat cttatgaaat ggacatcctc
    33661 tacaagcata tcagtgcata taagaagcat gcatccgggg gaaaccctca cgacagccgc
    33721 atcgtctccg attccctgaa ctactgagga cggatcattc acgcatccat catcgatccg
    33781 ggatatcagg ccgaactctc ctatcttcga aatctccatc gaaatctgct cctttgcagc
    33841 gctccatagc aaccatcaca cacaggcatc caagtgggta gagagtgcac tcgggggaat
    33901 acggctcatc aattttccac agatagaact atagcatttc tgtctcctga tcatgaccat
    33961 atcggacctg tgtataaacc aagaaggcga acatgattcc tgtagccagt ataataccgc
    34021 cgcgcttcga atgctctccc cacccacccc ctgcccggga cttatcgcca gtgccctggt
    34081 catacaaatc agtccaggaa atgtcggcat ttaacataga tcctttgcag ccggctatag
    34141 cccatatgcg atggtttgca gcaacaggag tgttctcagg cttcgcgctt tttcatgcgc
    34201 atgggcgctg tgatcctgcg tcgtctggcg gcatgaaact gggctccaac taacaggcct
    34261 cactcaatct gaacaaagcc ggtaatggga taccgggccc gccaggacaa agttggggct
    34321 ttgaaggtta ttttccaagc tgtagcaggc aataacggtc ttccagcctg gacaggcaca
    34381 tgctcctatg ccggaaacag gcttcggttt gatcacccga caatcccctt ccttcaggca
    34441 tggtgagtgt cacggcccgg aggcatgcaa tgtgacaagg acctggcgcc gccccgggca
    34501 aggcctgtgc agcccccgcg gggtcagacg ccatcctctg ccggcatgct ctctcccctg
    34561 atccggttca ggtaggagca agattacttg gcaaagactt tgactcctcc gacgaatgtc
    34621 atttcaacat ctatatcctt caggtgctcg ggaggaaccg acctagggtt ttccgacaag
    34681 acaactaaat cgccaaactt gccagggcta atgctgccac aaagattctc ctcacagcct
    34741 gcataggcac ctattgccgt atacgaacgg agaacttcat ccactgtcat cttctctgaa
    34801 ttaccgagtg tcataccgct cgaagtcttc cttgtcaccg ccgcatacat ccccagaaga
    34861 ggactgccat cgcacaccgg cgaatcagaa ttgccggcca cccgaatgcc tatgtccata
    34921 gcgctcctta gagggaatgt gcggcatgcc ctctcgtagc cgaggttcga tatccagctg
    34981 tcgcccaaag catacatgaa agacgtggac gcctcgatca ccagcccaag atctcggatc
    35041 ctcttcaagt ggtctcgagt aggcaatacg caatgatcta tcctgtttcg catagctcgc
    35101 acactctcta cggagacctc ccgcgcggca tcctccaaag aggatatagc aagctctatg
    35161 gccctttcac ctattgcatg gatcgatacc ctgatgccat ttttcacaca ctctgtcaca
    35221 aggggtgaaa gctcatccct ttcaacatag agaacccctt tttcaccctt tcggcctgca
    35281 taatcctctt ccacagctgc cgaacacccg cctatgctgc catccaggat gaattttacg
    35341 cccatgaact tcaacatgtc gtttccatac ccgctcttga gtccggatcc taaaacaaag
    35401 gggataagcc ccgcatcccg cgacccgaaa gtgatgcctt caagccacac gcggacccga
    35461 gcaggcaagc tatggctttc agctagtctt tggtaggttg cgaacgcgtc cgcatcgacg
    35521 agcatgtcgt ggactgttgt tatcccccac ttcggcaact gcccaagagc gagtgcgaat
    35581 ccgcgttcca cttcctgatc ggtgtacggc ggtatggcta tcagatcttg tgcacgatcc
    35641 atcaacaaac cagtgggttc accagtggcc ggatcgcggt cgatccgccc tccctcagga
    35701 tcaggcgtat ccgccgttat tccagacagc ctcagcgcga gactgttggc aacgcccatg
    35761 tgtacacaag tcctccttat atagaccggg ttgtcaggcg aaaccttgtc cagatcccat
    35821 ctagtcggat gcacgcgttc ctccagttta ctgtcgtcgt atccccaccc acgaacccat
    35881 ccgcccttcg gggtctcatc tacgcgttct gcaatcttat cgacaatatc ggccacagat
    35941 ttcacagaag ggtatgtgca atccacatcg agcatcattc cggccaatat ggacggatgc
    36001 atgtgtgatt ccacaaagcc cggcgcaacg gcccggcctt caaggtcaat aacctctggt
    36061 ctaccatggc atcgcgaaaa tgccccttct tccgcgccaa cgtaagctat tctgccgttg
    36121 cgaacgagta ttgcgtcgcc ctggaggttc ggttcgtcaa tagctagaat ttgtccgttt
    36181 ttcagcaata gctctttctc atcgttttgc actttcatct ctccaacacc atacttttcc
    36241 ttgtaaagca cgcgactcca tgctttatta taatccaatt cctctgttgc agagactggc
    36301 catcctgctt ttagcggcta aaatgctcat ctttctgacc ttccagacaa aaccctaggt
    36361 aatatgtaaa cttagctctt cacaaataag tccatagtag tattattagt tatagagtag
    36421 gaggctactc ctaatttcga ctcattacac atgtctggaa agggaagatt gtcatgaaaa
    36481 ggctgagtag gctgctcgca ttgttcctgg tagtcctgct gtcgttgtcg ctcgtcggct
    36541 gcggcaagaa agaagagccc gaagaggaca tcctggacaa cgattttgag gaaacggaaa
    36601 cctgggacta cgacgaagac gacactgagt ccacagattt cgacagtgac tcttgggatt
    36661 tcgactggga agatacgacc gacgaagaag acgatgatta cgattggggc ggatcgttcg
    36721 agtagttcgt ccggtaaaaa catactacgg tcttatgttg ccgtaatgca gttggataac
    36781 tgactagatc tattcgattt aggggcgtcg gaaggcgccc ctaaaacctt aactgggatc
    36841 aaatgatgcg cagccttcca cgcggctttc agcatatctt ttgctacgcc cgcgggaaca
    36901 atagtatggc tttgaggagg taggcacatg aactgcgcca atggctatgc gaatggcaac
    36961 gaaacccttc gtctgcttac gtcgagggcg agctgccgcg atttttctga caagaagata
    37021 ccgcaagaga cgattaatgc agtgctcaat gccggaatcc atgcgccaac cggcggcaat
    37081 ctacagccat attccataat aaaggtcgaa aatcatgaca tgcgcatccg cctggccgaa
    37141 ttctgcggcg atcagggttt cattgccaaa gccccggtca acctagtatt ctgcattgat
    37201 tggcaccgac tagaacggtg ggcatccttg gaggacgctc cgttctccgc cacacagtcg
    37261 ttccgccact tttggatctc atttcaggat actgtcattt gtgctcaaaa catctgcacc
    37321 gccgccgacg ccgtcgggct cggctcgtgt tacattggaa ccgtcctgga atgccttcgt
    37381 gaacttcgcg aactgctcga actcccaaat ggagtgcttc ccgtagtgtt gctggctatt
    37441 gggtatcccc gagaacgacc aatgccccgc aggaagttgg gggcagacgt tgtagtgcat
    37501 gatgaggtct accgggatct ggatgctgaa gaattgctgc gggcctatga aaacaagtac
    37561 gaagggtttg cccttgaagc caccgaagag cgagtagcaa ttctggagga agtatgcaaa
    37621 gcagtgcacg gcgatgaatt tgctgttgaa tgcctggaac gagtccggga agctggactg
    37681 ataaatgcag cgcaatacag gtttggcctg cactaccgtg cagacaaact gcctgatggg
    37741 aatgaggacc ttgtccgggc cactgaggaa gcagggttca actggttcaa gcgtgctgac
    37801 atgttcgtag atgacaagga ctgatattag gactctcgcg tcacatgcta gttgtgagat
    37861 ccaagtcctt tcaccggcag cccacagcct ggcataggtc gtgggctacg gttcgagcgc
    37921 ctaagaatcc ccaagtatgc gagtgtcaat tgcagcaacc tccgaaggct ggcatattga
    37981 cggcgacgcc ggcgcgtcgc ttcttttccc cagtcctcag agtggggcga cccgtcgaca
    38041 ggtcattctt gcagaagcga cacgcccaac taacggccaa tccacatgag agctttcatg
    38101 ggattggcga aactccgccg gaaacctcag cggcatattc acatcatgca acctctgtgc
    38161 ggcctcgcac ccagcaggcg cacatatgtc ctccgccaac atcctcgagc accggttcct
    38221 tttcgctgca tatgtcctga gccaaccgac atctcgtatg gaacctgcag ccgctcgggg
    38281 ggtcaaccgg gctaggaacg tctccttcaa gtattatcct ctgtctcccg cccctcagct
    38341 tcgggtcggg tatcggcacc gcagatagca gcgcctccgt atacggatgc agagggcttg
    38401 aacatagttc atccttctgc gccagctcta caatctttcc caggtacatc acggccactc
    38461 tgttgcttat gtgccgaact acagcaaggt cgtgggcgat aaagatatac gtcagcccga
    38521 actcttgctg cagatcctgg agcaagttga tgacctgcgc ctgaacggaa acgtccagag
    38581 ccgatacggg ttcgtccagg attatcagct tgggattgag tgcgagcgcc ctcgcaacgc
    38641 cgattcgctg gcgttgcccg ccggaaaact cgtgcggata cctgtgtgca tgcgatctgt
    38701 caagcccgac aactcccaaa agctctgcag tcctccgctc caattcggcg ccttttgcaa
    38761 tcccgtgaac cagcaagggc tcgccaacca tctcacccac ggtcatccga gggttgagag
    38821 atgaaaatgg atcctggaag actatctgca tatccttgcg cgctctccgg agatcttcct
    38881 tgcccattga aagccaatca cagccgtcga aacggatcga tccttccgta ggctcctcga
    38941 gccgcatcag cagccgcccg gtggttgact tgccacatcc gctctcgcct acaaggccca
    39001 aagtctcgcc ccttcgaact tcgaaggata ttccatcaac cgcccggact gcgcctactt
    39061 gcttctggcg aattatcccc tgagtaatcg gatagtgctt tgtcagatta tctacgcgga
    39121 gcagagcttc agctgatcct ttgctcactt ctggtccgcc tccttttctg ccctgataca
    39181 ccttacctcg tgcccaggag cgattctcct caagggaggc atttcgcgcc tgcaggccgg
    39241 ttgggcatta ggacatctag gcgcaaaccg gcacccttcc ggcattcgca acggactcgg
    39301 gacgacgcca tcgataggat ggagccgttc tgtctcttca ttcagcttgg gaactgaacg
    39361 aagcagacct gcggtgtagg gatgcaccgg tttctcgaat atcgtcatca catcgcctgt
    39421 ctccactatc tgggatgcgt acatcactac taccctgtca gccgtttcgg caatcacgcc
    39481 cagatcgtgg gtgatgagca tcaccgacgc tccgctatcc gatttcaact gcgctatgag
    39541 gtccaggatc tgcgcctgaa tcgtcacgtc cagggcagtt gtgggttcat ctgctatcaa
    39601 cagcttggga ttacaggcaa ttgccatggc gatcatgacc ctctgcctca ttccgccgct
    39661 catctggtgc ggatactcgt ccacacgccg ctcgggcgaa ggaattccca ccttccgcaa
    39721 catctctata gcgagttttc ttgcagtacg cttgtctacc gcttggtgaa gcagtattgc
    39781 ctccataatc tggtcaccca cggtgaatac ggggttgagg cttgtcatcg gctcctgaaa
    39841 tatcatggat atctcgtttc ctctgatctt ccgcatatca tgttctggta tggataacaa
    39901 atctcgtcct ccgaacatca cctgcccgga agcgatcacc ccgggaggac tctgaacaag
    39961 tcgcattacc gaaagggatg tcacgctctt gccgcatccg ctctcgccga ctatgcctaa
    40021 ggtctcgcct ggccatacat caaagctcac gtcatcgacg gctgttacct ctccgtcttc
    40081 agtcatgaag cgagtgatca gatttctgac tgataacaac ggttcgtgca gttctggcac
    40141 tcttgtgccc ccccttagaa tcctttcgca ttgagaacta caagaagcgc tccatattga
    40201 acctcgatca gatcctttat ggggaccact tcgtatgcaa gattctcttc tatatcagtg
    40261 gcccatctgt caatatgggc attgaggtcg tacagaacgg accgcaggat ctgcctttta
    40321 ggcgcacagc tgcatccata tcccccatcg cagcattcct cctgactctc cgcaccaagt
    40381 tcgcccgcta tggctcgagc caacatgctg gcgactatca tcttgtagaa cacgcccaca
    40441 tagatcgagt caacctcctg agcaactgta gcaggctgat ccatcccgga agtctcccgc
    40501 gcccaccgct cttggcttgg aatggacttc agttccgaat cgacgaatgt ttcgactgca
    40561 cctcgaagga gctttccctc aggccttgtt atcagacctt cagtcgcggt taggtccttg
    40621 gcaagcaact cgtatatgcg cctaccccta tctatgccct ccagaaccac ctctcgtctc
    40681 gttcgtccaa taggagtctc gtcccctatt ttggcagaca cgaaatacgg cacttcagtg
    40741 acgatcgaaa acggatcaca tactgtcttg gcccactcat cgctgccagc tccagccgtg
    40801 atatactgtg ccgggtcgcc aggcgcgaat tgctcgtagt aatcatagga agccgttatc
    40861 gggcacgatt cgtacactcc cggatagagt tcaaccgccc aaggcacttc aggctcaccc
    40921 aatgagagcg gaaggccgcg atcgatcgga attctgtgga gttcgtcgta gtcttccttg
    40981 ctcagcccgc tcgatatata gtaataagct cctccgaagc cggcgttgtg cagcgagtac
    41041 atcacgtccg gacgtgtcgt ctctattgcc ttcatcaagg cctgcgtctc aggtttgggc
    41101 gtatcccatt tgagagtctt gtattctatt gggaaagtcc actccacctg atcctgaggc
    41161 cgcggcctat agaaatcgtg cgcgtagttt ctgatgttga atgggcctgc aaaccatcct
    41221 tcgttcatac gcgtcccgtc aggatcgaca cacggcatga tatgccacgt gcgacccgca
    41281 cgcaggtcct catccttcag gagctcttcc attaggaagt aagtcatcat cgccccgatg
    41341 ggttcgttcg ggtgcggaca ggcaaagatc aatatgctca caggcccgtc gcctatggag
    41401 accataggta tctcgctgcc ggctgtagat tttccgacaa tggccagttt tgcaatgtca
    41461 ggacgggcct ccgccaccgc cttggcgcgt gcataaagct cgtcaactgt cacaaatcgc
    41521 gtgtattcag ggactgcagc caatcgttct tctatcgtca agtctgcttt catttgcaaa
    41581 cctcccaagc tacatatagt gctagtgctt tcgcatgccg taaccttcgc ggcattggtg
    41641 ctatccactc gactgcgttc agttcagctg tttggcaagg cgcggatcga gagcgtcccg
    41701 caatgcatcg cccacgaagt tgaagctgag aacaaccaga aaaatgcaga ggcccggcca
    41761 gataatcatg ttggagttga tcaacataaa atccttggcc tcgctcacca tcgcacccca
    41821 ctcaggggtt ggcggctgaa ctccaaaccc cagaaaaccc agagatgcag aggaaagtat
    41881 cgcgctgggt attccaaggg ttgccgagac tatcacgggc gtcagcacgt tggggagtat
    41941 gtgcctgaac atgatcctgg cgtcgctaca tcctaacgcg ctcgcagctt taatgaaatc
    42001 ttcggattta agggccatta cctgggccct caccaaacgt gcgtacagcg gccagcttac
    42061 aacgcccaac acccacatga cattgaatat gctggggccc aaaatagcca tgaccgctat
    42121 cacgagaatg agaaacggaa atgaatagaa cacctcaacc agacgcatta ccaatgcgtc
    42181 aacccatccg ccgtagtaac ctgcaaccat gccaagcaac gacccgagaa cactcgatat
    42241 cgacacaact acaatgccga taagaagaga tattcttgag ccgtatatga gccgtgacag
    42301 catgtctcgc ccaaagttgt cggtgccgag gatgtgtcct ggcgagcccg gcggcaggaa
    42361 tggattcgca aggtccatct catatgggtc acatggcgca acgaccggcg caaagatggc
    42421 tatggccacg aatgccacga gcatgaccag tcccaaaacg ccgagcttat gcctgaagaa
    42481 cacttgcacg acatgggcaa actcagacct tatcgttccc tctgtttcct caattctttc
    42541 tctttcttta ctcatatcta atcctcggat ctatgaatac gtatactata tcgacgatga
    42601 gtgtaatcag cacaaagctg agagcgaaaa caagggttgt cccctggatt accggaatgt
    42661 ctcgctgttt gactgcgttc acagccaaca tgcctatgcc cggccacgaa aagatcgtct
    42721 ctgttatcac agctcctccg agcaatgatc cgaattgcag cccgaaggtg gtaattacgg
    42781 gcaacagcgc attgcggaga gcgtgcttgt aaaggaccgt cttctcacgg acccctttgg
    42841 ccctggcagt acggacgaag tcttgtgata gcgcttcaag cacggacgat cttgtgagac
    42901 gcgtcagaat cgcggccatt ccactgccca acgttataga tggcagtatc aagtgtttga
    42961 tatactccca agcgcccgat cccgcagcgc ccattcctgt gatgggaagc catccgagcc
    43021 tcactccaaa aagaagaatc agcagaagtc caaggtaaaa ccctggaagc gagactccta
    43081 gcattgcaag ggtcgtcgct gtgtaatcta tccaagtgcg cctctttgca gcggccaaca
    43141 cgcccaacgg gatcgagatc agtagcgcaa atatcgcgga cgatacggca agaatggccg
    43201 tgtttgcaaa cctctctttg acctgccctg ctaccggacg attgtagact atcgatcttc
    43261 ccatatcgcc acggagcaat ccactcatat atcgcaaata ctgctcgtac catggcctgt
    43321 cgaggcccaa ttctatccga attcgctcgg cgtattccgg atttacagtc tccgatccga
    43381 gcataagctc gatcgggtcg ccgggagcga aactgaccat cgcaaacact atgaaggtga
    43441 tgccgattat aaccggcact gtcgccaaaa gacgacgtac aatatatctg gtcaactctg
    43501 accttccccc ctaacttcac aggggagggc gctacatttc aaggctgcgt cgccaagcaa
    43561 cgtatccctc cccatcgaag cttcctgggt tcatcttccc gtacgaatcg atctgtcagg
    43621 aatgaacgct actttgaaat ccagacgttg ttctcgagtg tcactaggtg gagaccgccc
    43681 cacggcgaca cgtagtcatt gactttcttg tttacaaccg agtatcccca ttcgaagtag
    43741 agcggtatcg aggccctgtc ctccatgatc ttgcgttgcg cctgcttcca gaggctcgac
    43801 agcttcttca tgtcagtggt ccttagcgcc tgatcgagca gggagtctac agtggaatcg
    43861 gcatagaagt ggccattcgt cttgccaatc gcctcactgt ggaagagcga atgcagacca
    43921 gtggttcctg cgaaactgaa gtcgaagaac acgccggact tcccgcccat gatgtcagac
    43981 acccaaacgg ccgtatcctg ctgcactact gatgcatcta tcccgatctg cctgagctgt
    44041 gtagcgagga ttgtgagcac cctcacttgc gaaccgggga cagtctttat ctccactttc
    44101 aagggctgcc catttcggtc aagtattcca tcgccatttg tgtctcggaa tccggcctta
    44161 ttcagtattg ctacggcttc cttagggtca tatttccaca ggctcttcag acttggatca
    44221 tagccatggg caacccaggg agggcactgc ccatatgccc gctggccatg gggagctacc
    44281 actgcgttga acgcggcatc aatgttcatg gccttcgaaa tcgcatcgcg caccgcgaat
    44341 tcatcgaaag gcgaaacagt cacgttaaaa ccaagacccc tatatgagcc gccacgacct
    44401 ctcacagcag ccagcttgcg gttcttcgaa atcgtgggca tggcgtccat gttcacaaga
    44461 tacgtgatta tgtccacttg ccccgaagta acggccatta cctgaaccgt cggatcaggt
    44521 atgacgacga attcaacttt atcgagatat accggcaacc agtagcgagg attccgttca
    44581 agaacagcgc ctttatcggg tgaaaagctc ttgagctgga atggccctga gcctatgggc
    44641 tttctggcaa agccatcttc gccaagcttc tcgattgcct cacgaggcac tatgcccaca
    44701 cttggcaatc ggttgggatc gctgaccaga aacggcgacg gctcaggcgt cttgatctcg
    44761 acagtataac gatcgagtgc cttgacactc ttgattgggc caagggtgaa agcagtacta
    44821 acgttgatga aacgattaat ggaatacacg acgtcgtccg ccgtcacttc acgtttggcg
    44881 ccctttttga aaacctcatt gccctcctgg aagtacacgc ctttgcgaat atggaaaacc
    44941 caagtgttct catcaggcat ttcccaactt tgcgcaaggt ggggtttcgg ctcgaaacta
    45001 tctttgccga taaccacgag gggatcgaag acgaggccgc acagattagt ttcgtcattg
    45061 gcggcagtct tgtatggatc aagctgttgg atggatgacg ccgcgatctt gagagtgccg
    45121 cccttagtcg gaccggctcc caaaaccacg gttgatgcag acaccaatag aagtgctgcc
    45181 acgattgcta gaactgtcct tttcgacatg tcctgttgcc cccttgctgc atctaatgat
    45241 aaatcgttaa tagatattag tctgtaatta tacgtgtgcg agctagataa gtcaaacaga
    45301 aaattattca caataattgt acaaagaatg cttataataa tggggcacgg cccaacgcac
    45361 atcgtatgtg tctggacaag cctcgataga gtttcttgat aatgctgggg cgctctttgc
    45421 ggcttcctag ggaacaccat aggtcgccgg ctagcgatgt cactgcgtga tacacttccg
    45481 ctaggccctc aggttagagg ctttcaagcg ccatcatcag ttttatagac tgtcgtctgg
    45541 gaaacaatac aagaatgaaa tttggaagcc aatcgggggg gatattcggt cgccattgtc
    45601 ccttcgccaa tagcaattac atgccaaaga tacaacggcg aatcgccaga accacaccgt
    45661 catcctcgtt ggattcagta acaaagtcgg cagcccgctt cagctcttct gcggcattgc
    45721 ccattgccac gcccaatcct gcgccttcaa tcaagtccag atcgttgaga ccatctccaa
    45781 cagccatgac cgcctccatc tcgatgccta tctgttcgca tattctcctg actcctcggg
    45841 ctttcgtgat tccccgagca gtgacttcca tgttcgcatc agacgtgcag gttacctcga
    45901 ccgtccccca gctatcaacg tagtcgcgta attgggccat taagtcgaca tcgtcgcagt
    45961 acatgccgta cttcatccac tcttgatgaa gcatgtcgtt actccagtca ttctgtatta
    46021 gggtctccac ggtgtatccc caaaagcagc atccagtctc cgccgtcagt tggtgtaacc
    46081 ggtaaatgtc ctcagtatcc atgaagtgcc tttccaacag ctgtccaggt ccaccccaaa
    46141 tctcagcccc attcaagaac accatcggcg aatcgagatc gagctcacgc acgaatgaaa
    46201 gcgcgtcttg ccttcccctc cccgtggcaa acgtgacgat gaccccggca tctgcggctt
    46261 ttccgaccca taacttcgta ctgtccgcaa ttcttttgtc gtctgtgagc accgtaccat
    46321 cgagatcgag ggctataagt ctgtggcaga gcatattcca actcatcctc cttgcagtct
    46381 catgcagaac gcagtgcatc cgggaaaccc atcctcagac acttcccaac tccatcataa
    46441 ccaggccctg cgctgcatgt agactatatc acttccgact ttagaggcaa agtgctgcca
    46501 tgcccccggc accacttcat ccgcctgacg caagcaggga ttgaatcggt aaaagagaat
    46561 attcgtcgga aggaggtgaa gcagtgacaa cttccgcact tatcttggta ctcgtttcgg
    46621 ctgtgatcca cgcaggctgg aatctggcga ctaagaaagc tgaaggcggc gcggcatacg
    46681 tgtttctgat ggctgccggg tcggcattgt gttggtttcc cttggcagcg tggctgttga
    46741 tgaccgggaa cgtgaccacg ccaggagcag gagcatcccc tttctctgta gtcgtcgcaa
    46801 tcagtgtcag tggagcaatc catacagcct atttcctgtc tctacaagcg ggatacggat
    46861 gtggggatct atccctcgtc tatccgctgg cgcgcgggac aggcccgctg ttatccacgt
    46921 tggcggcgat gcttttgtat ggagagcgcc catcaggatt agcgttggca ggcgcattgc
    46981 tcataggagt aggcgcattc tttcttgcag gcgccgagga gccgcaggct acgccttctg
    47041 atgacggcag cagcaaagca atagtgtacg ggctcctcac cggggttttg atcgcaggtt
    47101 acactatgtg ggacaagtac gctcttgatg tggtcggcgt caaaccggcg tttctgtcgt
    47161 cggctgccaa ttgtgtgcgt gccataggaa tggcgccgtt tttcctatta tcgaagaagc
    47221 gtcgagctga agtgagcacc gccatgcgca ctcaatggaa acctgccctc atagtctcca
    47281 tatcatcacc cctatcgtat cttttggtcc tcacggccct ggcaacatcg cctgtaagct
    47341 acgtcgcccc ggcacgggaa atcagcatcc tcctgggcac acttgccggg actcggatgc
    47401 tcgaggaagg agaccccagc tcaagaaaaa aacgcttgtg ggcatctgtt gcaatggcgg
    47461 caggcgtggt agccttggcc attggatgag agcaactaga tgtcatgtta agtcaggcac
    47521 gaatcgcata ctccacatcc cgcatgaaaa ggtaatatat attgaagcca acccgatctg
    47581 taaccggcga gagagctgat agccccgctg gctttggaag tgacattgtg gcggtttttc
    47641 gacatggcaa cgggttcggg ctccatttcg acatgtgccc gatcatgatc tgctatagca
    47701 tgccatctta agccacacca tggaatatac atggctgcat aatgatcgtt ttgggcaggg
    47761 atttaagggc gcatgcccgg ggcctgacct gttgacccaa attgagctcg cgtctgaaaa
    47821 aatggcatag ggtaggcagc gaatagtctt cttcttggca agtcacaaga agtgtcccgg
    47881 ctttcattgg gcaactgggg cctgaaagaa ccctcaagga gcatcgcaaa atctatatgc
    47941 agagactgag atgggcaaag atggggaggt gcattcgatt tgcaaattcc gtggaagagc
    48001 atcgtcatcg tcatattcct tgtccttgct ggtcttgggt tttatgcaag cggtccccta
    48061 gcacgagctg atactgtgac gcaggttatc cgtgaatccg tctggatcga agcgcccaac
    48121 cgatttgaaa ttcagggcaa gaacgagtgc acagggtttg cagtcgcgta tgtgctccgc
    48181 cacttcgaaa tcgaggtaac cggcaaggaa atctacgacg ctgtcaggtt caagctcccg
    48241 tctggcaggg tcttgccgag gggacccatt aatgagcttc gtcgacacgg catcca
//
LOCUS       DAOSYL010000009        46864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_635_length_46864_cov_8.420005, whole genome shotgun sequence.
ACCESSION   DAOSYL010000009 DAOSYL010000000
VERSION     DAOSYL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 46864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46864
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_635_length_46864_cov_8.420005"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1074
                     /locus_tag="PLU15_02990"
     CDS             271..1074
                     /locus_tag="PLU15_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOO29920.1"
                     /translation="MLPTRLLSVQRSMALMAVALIVAVPGLAALSESDAADGPSRVVT
                     FHPRTGYTEDVLEGAGVSRPARTSAVAYVATGWVTKHIVTRKVPVERPIERIIDPKMD
                     RGVSRVVHEGSDGEMMQAVLVKRGENGVISETVISERLWTRPQPKVIATGSREPLRVL
                     LTSRGDYTYRKVLTMTATGYEPSPRSCGRYADGYTAIGMKAAPGVVAVDPKVIPLRTR
                     LYVEGYGPAVAADVGSAIKGNRIDLFFETLDEALRYGRRRVNVYILEDR"
     assembly_gap    1170..1269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1270..1708
                     /locus_tag="PLU15_02995"
     CDS             <1270..1708
                     /locus_tag="PLU15_02995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HOO29921.1"
                     /translation="LTLMVQLEVAQKLTAKPGSRAYGELSVKTQYFTEPVLLFEVPPT
                     AFDPPPSVRSAVVQMTMRSAPPVEADADMMFSIVSAGYGKRRKTLRNALLASHLFAGD
                     RDAVEEVLSCASVDGGRRAETLSLEEFAAISRAYVAYRAGENG"
     gene            1705..2178
                     /locus_tag="PLU15_03000"
     CDS             1705..2178
                     /locus_tag="PLU15_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HOO29922.1"
                     /translation="MKYFISPTKGRLTFEETFDDIMAFVHDMPDEEYKLIIGTDSQVN
                     GDTCFVSAIVIHRVGRGARYYYCREHETIGTGLRQRIFYEASKSLDLASQVAHKLAEN
                     GYADVNIEIHLDVGVNGKTKELIRDIVGMISGTGFSARIKPDSYGASKVADKHTR"
     gene            2473..3426
                     /gene="yabG"
                     /locus_tag="PLU15_03005"
     CDS             2473..3426
                     /gene="yabG"
                     /locus_tag="PLU15_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOO29923.1"
                     /translation="MANRNRIREGDIVTRKSYGGDVFFQVIRVDSYAVGDATATLKGL
                     NMRLLADAPISDLSIVEERQLLDFKRKVMRESAEHLRTVRVRRITEEQARYKKERRRS
                     RGSAALAEDIFDLPGRVLHIDGDADYLRDCMKYYKELGVPVVGEHVPPQDQTAAVGPL
                     LDEHSPDVLVLTGHDALKKKNSDRASMSSYWNSEAYVNAVRVARQHEMDKDGLVIIAG
                     ACQSFYEALMEAGANFASSPGRVLIHCLDPVLLAEKVVNTPVEEIIRIEEAIENTITK
                     RPGVGGIQTRGKMRTSMPKTPMGVFGAGANRDNQSARNYMP"
     gene            4032..4310
                     /locus_tag="PLU15_03010"
     CDS             4032..4310
                     /locus_tag="PLU15_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOO29924.1"
                     /translation="MAGNNVLDRIREDLDSFVGKPVKLRANQGRKKILEAEGVLEQTY
                     PRVFVVRIDEDPRSVKRVSYTYADVLTSTVEVRVKNKGMETRIGCAGS"
     gene            4563..5582
                     /locus_tag="PLU15_03015"
     CDS             4563..5582
                     /locus_tag="PLU15_03015"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOO29925.1"
                     /translation="MTHEYRGVIHVHSTFSDGRGDIDQIMLEANHADVDFLILTDHNT
                     IEAKLRGYEGWHDCCLLVVGEEITPKTKADHYLALDIPCAIDPSDDPAKNIAAVRKAG
                     GIGIIAHPTGGAVVKGEYLEYPWTDWDAGPFDGIEIWNHTYDITGKAKNIFELIFYVL
                     FPKAAKAGPIQDVLDTWDRYSLSQSRRIIGIGGTDSHGLLSRYSDDFKTVCTYILAKE
                     AFNYDAAHDIALVYEALRAGRCFVGADKVAKASGFKCEVVCGDNAVQMGGRACLSRGA
                     CLNVHAPEPGLIRIIHNGDAILEEKGVDLEAALESPGVYRVEVKRRVYGRYVPWIYGN
                     HFFLA"
     gene            5549..7048
                     /locus_tag="PLU15_03020"
     CDS             5549..7048
                     /locus_tag="PLU15_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOO29926.1"
                     /translation="MDIWQPLFPRMSTTKVQARLTCLGFDPGPCDGKYGPFTRDAVRQ
                     CQAAYRLVADGVLGPEIKRLLSQDLPSCRVTVRAVEGQSLSGVARALGTTVEALVEGN
                     RRKRYEDVFPGERLMVHKRAVGAFMYPLAGAEGGTVDLDITFELPWTFALFPMFAIAD
                     TGEVRSVMSPSPLAVGLTERENGRVDSIRVSRSLRGRKQGAEPLAIPVAKKSHADYGS
                     PVGSSVQFLPGARESAAAAKTVRRPGGTQFFAMLSGPADINCGTLALLRSGRAAIVST
                     TCSIAREAAASGAAGVAVNIPGLGDDECSMYSRFVRCLSRECHRLGMKLAVIIPAPSW
                     QADGQVHFQGSSAPERRLPWPNGYDVELLGSLADLVLLDIADPSAFWDSIVLACRHVP
                     RWKLAAVLELRAYRQEPDGHVPMSQEELRAFMARHVVRGGRDEFSRVPYLSYRARGVV
                     RRIWQEDAESLGKKLHAVNRLNILGVVFKGARDAREQVLDEVCRRFIIM"
     gene            7110..8720
                     /locus_tag="PLU15_03025"
     CDS             7110..8720
                     /locus_tag="PLU15_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO29927.1"
                     /translation="MGIQLEHRQLSITRVAAQGMAEISLQRSVALLQDHPDISDDSAH
                     SILWAQGVPIVASVEPMDGQIKVTGYVQSTVFYVAKGAPDFVGKAVMSEPRFEAVIPA
                     PGATVSDRVKVDLTLSQLEVETTGTRGLLLAATIMASARALRDEVVEAAVSAEATGGS
                     RISVHSEEINVTSIHESFSEKVEFSEILSIPDEKAACLGSESACGAAGTARVIGIQVG
                     DGKVTLDAEISVEIASTVVRGVPALNILRFERVPIHASFDVPAAEKGMTALTQLALSH
                     VSAILLSDAEVSIDGILDVDVDLVSSERLSVIVDIDSETQEIVDIDTAGITVEKLVGE
                     GMIDLDLHDSVALQALMRRYSFTGMEDVEGSIGLLGLSDIETTDDEVSLRGLLTARAI
                     LSDVDEDEEGQFPINFALDIPIEFSDSAEVPGVRAGDRAEPRSSTHTVVVGKSAPNRL
                     DVEGSLRVDVEVYRPVELQVVTSAETISPVTLDPYAMTFYVVGPGDTLARIARRYGVP
                     PDRIAAANGMSVTEEVTPGQKVYIPTRR"
     gene            9066..11531
                     /locus_tag="PLU15_03030"
     CDS             9066..11531
                     /locus_tag="PLU15_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO29928.1"
                     /translation="MDASKFINSVMSEYDYRDQVVHVETIPPREARYAQLSPPVAPPI
                     SSSLKEMGISELYTHQAEAVSRARAKQDIVVVTSTASGKTLCYNIPVIEALMADSSAT
                     ALYIFPTKALAQDQQRGLVRFVELEPDLPIRSGTYDGDTPDSTRRKLRENANVILTNP
                     DMLHQGILPSHPSWRRFFAGLRYVVIDEIHAYRGVFGSNVANVVHRLRRVCAHYGSDP
                     TFICCSATIANPGELAGAISGKDVVVIDNDGAPRGPRKFVFWNPPRLENTIQRRSSNS
                     EAERIMVKLIESKVPTITFVRARVVAELIYKYAVESLQRNSPSLAKKIKPYRGGYLPS
                     ERREIERQLFSGELLGVVSTNALELGIDIGSLDASIIVGYPGTIASTWQQAGRAGRKG
                     EESLSVFIAHDLPIDQYLMGHPEYFFDRSPEHAVVNPANPYILVGHLRAAAMELPISP
                     LELADFGEYAPALIELLEEKGEVLRTRLGWRWIGRGFPANDVKLRNMSENTYTIVDTS
                     SDHGNRVIGTIDELSAFEQVHPEAVYMHEGETYLVSDLNLVEKVAYIHKADVDYFTQS
                     VTETRVQIEEKEQNKMWRKSEVEFGDVTVTNLTYMFRKIKFYERDSIGYGKVNLPENN
                     LPTTAAWIMLPESAAKLVASLGRIPAEGLIGIGNAASAVIPLFAMCDSMDIGTAVDSS
                     NTGVPTLFIYDRHQGGVGFAQKAYKMIDEVMEACLNLIQNCPCEDGCPSCVGSPIPPY
                     AQNDPDATSRGRIPDKDTALIILHNLLEKEPYVPAGPPKWAEGSDNADGACAGYSEED
                     FRIDVKPLPDGVERRIRKMINSR"
     gene            11965..12708
                     /locus_tag="PLU15_03035"
     CDS             11965..12708
                     /locus_tag="PLU15_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO29929.1"
                     /translation="MKRLLALACLCSLVVVAFAAPGVGAAKPVLKVGTSADFPPFEFQ
                     DEKTGAYLGFDLDLIRAIGDAIGMDVQIVNTAWDGLIPGLITGNYDCIVSAMTITDER
                     LKAVNFSDPYFSAGQVVVTMAKDTKISSIADLEGKKISVQIGTTGDLVASEIKGATVK
                     RFNLAPDAVQEVRNGAAHACIIDLAVAAEIAKEYKDIKYGAPFTMEYYGIAIKKNNTA
                     LLKNINRGLAMIKASGKYDEIYNKWFAGN"
     gene            12882..13517
                     /locus_tag="PLU15_03040"
     CDS             12882..13517
                     /locus_tag="PLU15_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOO29930.1"
                     /translation="MPALLAGAKMTIELAVISVAIGFVIGSLVGIARVSQNRALRVIA
                     GIYVDFIRGTPLLVQLFLVYFGLPTLIGRPVPPFIAAISAMGINSGAYVAEIVRAGIQ
                     SIDRGQTEAARSLGLTSSQTMQYIIFPQAFRRIIPPLGNEFIAMLKDSSLVSVIALED
                     LLRKGQVVITRTFRPFEVYTVVALIYLVMTFVISKLVSWSERKLKAVGQID"
     gene            13510..14232
                     /locus_tag="PLU15_03045"
     CDS             13510..14232
                     /locus_tag="PLU15_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOO29931.1"
                     /translation="MIKTVGLRKSFGHNEVLHGIDATINPHEVVVVVGPSGSGKSTFL
                     RCLNLLEKPTAGEIYFDGQLITAPGVNVNEIRREMGMVFQSFNLFPHLTVLDNIALAP
                     KIVRHVSKDEAEETARDLLRKVGIADKESAYPQELSGGQQQRVAIARALAMKPMAMLF
                     DEPTSALDPEMIGEVLDVMKALAVEGMTMVVVSHEMGFAREVADRVLFMDAGLIVEEG
                     PPAQVLGDPKNPRTKAFLGKVL"
     gene            15082..15843
                     /locus_tag="PLU15_03050"
     CDS             15082..15843
                     /locus_tag="PLU15_03050"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOO29932.1"
                     /translation="MKGRHFGSRLSLLAVICAFCLTTSGCNMGDYTFVDLPVRVAWEP
                     DQLIRVIDSEKVFGHRLGRGDGLRVWLDLGPLPCDLEPESIERATLKLPCQSFSRVAG
                     AVTFEIYMVKWTYDSGMGEWPDIPEFLHSSELPNDVQPISSARLSHDVGWETQHSNGY
                     VYSSQYIDTEVKYLSFDITEAIKAWVSGTPNAGLRIKQKSPARPDGPVWSLLMAPDKR
                     LKDIHKALDELPKSANGPSDQPIAIVCIKKAGEAS"
     gene            15952..16716
                     /locus_tag="PLU15_03055"
     CDS             15952..16716
                     /locus_tag="PLU15_03055"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO29933.1"
                     /translation="MEQAKRVFVRTKTADRASGATERASSEGHKRRWKCDWAAPRNRL
                     KSCPRGMRLSQRPDSGKGVILTLARIGAWFVLIGLILGVVSGGLFVGPFGGYIKKPPR
                     APLDSEHGDGLLPVKPSPGYRAPNFVGRLPDGTIISLDDCAGMPIILVFFRITSAPSI
                     AQMPAVDRLYRERQDRCCVIAVAVFEREDDVGRYMAANDLDIPMIADIRGEIAQAYDI
                     TALPTTFIIDGQRIIKDLRIGAMTYAELERALKPLY"
     gene            complement(16700..17584)
                     /locus_tag="PLU15_03060"
     CDS             complement(16700..17584)
                     /locus_tag="PLU15_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO29934.1"
                     /translation="MTGSLVFEFFRRIATPGLDRVVKIITDLGSISFYMIAIPIIYWC
                     IDKSVGFNLGMVTSISSCINVGLKFVFKVPRPYVAYPHLDAPNFLLDTGTGWSFPSGH
                     AQGTSTFWAYSAWHVKRAWFTALSVIVVAVVGLTRIYATVHYPKDVLVGTALGLIIVQ
                     VYMLVEESLANREKKTSVNTAVAASIVIPGVIFALSSLSFELARESASLLGFTMGLGV
                     GYALEKEYVKYDVRASFFIQVIKVVFGLAVLMGIRYGLKAILPEGSFWQMLRYAVMAL
                     WGTYGAPLVFKAMFSKEA"
     gene            18262..19161
                     /locus_tag="PLU15_03065"
     CDS             18262..19161
                     /locus_tag="PLU15_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOO29935.1"
                     /translation="MILSLGDVMIDAYFFTPNSRYPRTDTVGCVELAAGGSAANFAVW
                     VARYGNASGLIGRVGDDFLGHALVKELESEGVQPFLVFDPECATGRVGVIISEDGERD
                     MICDRRANTRLSPDDIPEEVIRQAEWLHISGYAFFEPGPARAARRAMETSLAAGIPVS
                     VDPAAYSFINAVGRSDFLKLCAGATVILPNLDEGRAMTGFSDPLDIANSLVEYFPVVA
                     LKLGADGCLGVSRHEPEPIRLPPIPTEVVDTTGAGDAFGAGFVMAYSHGEGLAASLAR
                     GNQLGATAVSRVGARSREALDLA"
     gene            complement(19153..19593)
                     /locus_tag="PLU15_03070"
     CDS             complement(19153..19593)
                     /locus_tag="PLU15_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO29936.1"
                     /translation="MGIFNPALAIAAGSGIAMAFQGVVNSTAAKTIGLWETTFVVLAS
                     GALLAGAGLAAGLNSGGLARLGAAPWWSLLGGPVGPLITIAVAYGIRKLGAVNATTAI
                     IVGQIGMAAIIDHMGWFGVRPIAFSPIKILGLTLLTVGARILLS"
     gene            19813..21423
                     /locus_tag="PLU15_03075"
     CDS             19813..21423
                     /locus_tag="PLU15_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO29937.1"
                     /translation="MRIRKSLLWFACFMTVLLIASTGFAAEKLAKKQILSFNVGTEPS
                     TLDPAKSTGIPEAIIELNCFEGLTRMDENNVPQPAIAESWTISKDGLVYTFKLRKTKW
                     SNGQPVTAHDFEWAWKRALAPETASEYAYQLWYIKNGRPYNEGKLKDPSKVGVRAVND
                     YTLQVVLENPTPYFLQLCNFPTLMPVYRKVVEADPEGWFQNPKNYIGNGPYKMVSWQH
                     NSKIELVPNPHYWNRKKVGMERLDLYLVDDIQTALSMFETGQLDWFDAPPAAEIERLE
                     KQGLLHKSEYIGTYYYMFNVTKKPLNDVRVRKALTMAIDRQTLIDRVVRGGQLPALAY
                     VPYGLGDATPDKDFRKVGGDYFKEDLAAAKKLLAEAGYPDGKGFPTLTILYNTSENHK
                     KIAEAIQEMWKKNLGIDVRLTNQEWQVYLDSRTQLNYEVARAGWIGDYLDPMTFLDMW
                     VTGGGNNDTGWGSKKYDELIYAAQAELNTAKRAKMLHDAETILMTEMPIMPIYFYVNL
                     TVIKPWIKGAFRSPLGFIDFSTAYVLEH"
     gene            21534..22463
                     /locus_tag="PLU15_03080"
     CDS             21534..22463
                     /locus_tag="PLU15_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29938.1"
                     /translation="MGSYVLRRFISVILVIFVVATATFFLMHAIPGGPFKREKALPPA
                     IQRNIEERYKLNDPLWKQYVDYIGNLLRGDLGPSYKYLGRSVNDIIREGFPISAHLGL
                     VSVVFALLVGVPCGIISALKQNQWQDNFIMAFAIIGVSVPSFVMATLLMYVFAVKLQW
                     LPAAMWGTPKHVILPMLALAGFPTAFFARLMRSSTLDVLSQDYIRTARSKGLSWGAVV
                     YKHVIKNAILPVVTYLGPLIAGIITGSFVVENIFAIPGLGRYYVTSIYNRDYTTIMGV
                     TIFYSALLVMLNFLVDIAYCWIDPRIKLTDAKE"
     gene            22470..23384
                     /locus_tag="PLU15_03085"
     CDS             22470..23384
                     /locus_tag="PLU15_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO29939.1"
                     /translation="MEYSAEMFKPINKDIDSAEAIVRPSVTYWQDAWRRLKKNRMAMV
                     GLWTIVAILVIAIFGPMVSPYSYSDQNLEEQNQYCSSKHWLGTDDLGRDLLTRIMYGA
                     RISLTVGFLASLLNLTVGVIYGGISGFYGGKIDNIMMRIVEVLNAIPMLLYVILLMVI
                     LEPGLQNILIALGLVYWLGMARIVRGQVLSLKEQDYVLAARSIGASNWQIITKHLIPN
                     AMGPIIVTATLNIPSAIFTEAFLSFIGLGVNAPMASWGVLASDALQSFRSYPWQLFFP
                     AIAISITMFAFNFLGDGLRDALDPKQRK"
     gene            23415..24425
                     /locus_tag="PLU15_03090"
     CDS             23415..24425
                     /locus_tag="PLU15_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO29940.1"
                     /translation="MDRLLEVRNVSTSFFTYSGEVKAVRDVSFHVDTGEAIAIVGESG
                     CGKSVTALSIMQLVAPPGKITDGEIFFNGRDLLKLSENQMQHVRGNEIGMIFQDPMTS
                     LNPVLTIQTQIVESIMLHQHVTREQAVKRCVELLHMVGIPSPERRLGSYPHEFSGGMR
                     QRVMIAMALACNPMLLIADEPTTALDVTIQAQIISLMAELKKKLNMSIILITHDLGVV
                     AGLAQRVLVMYAGKVIETGTVRDIYYHAQHPYTWGLLKSVPRLDAEEKKKLVPILGQP
                     PDLISPPTGCPFAPRCDYAMSICANEPPDYTKLSDHHKVACWLQYPLAPTYDRVERGG
                     VA"
     gene            24425..25396
                     /locus_tag="PLU15_03095"
     CDS             24425..25396
                     /locus_tag="PLU15_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO29941.1"
                     /translation="MEPLVEVKNLKKYFGVGGGTFKSKEGTLKAVDDISFSIPRGETL
                     GLVGESGCGKTTAGRTICRLYEPTAGEVIFDGIPIFSLGPKEILPIRRKMQMIFQDPY
                     ASLNPRMTVGDIIGEPIDIHNLASSKEERLDMIHELLHTVGLSPEHATRFPHEFSGGQ
                     RQRIGVARALAVEPSFIICDEPISALDVSIQAQVINMLEELQEERDLTYLFIAHDLAM
                     VKHTSHRVAVMYLGKIVELADSVELYKNPLHPYTKALMSAIPIPDPDVEAKRERIILE
                     GDVPSPIDPPPGCRFRSRCSYAMKVCGEEEPELRDFGGHHIACHLVK"
     gene            25598..26830
                     /locus_tag="PLU15_03100"
     CDS             25598..26830
                     /locus_tag="PLU15_03100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOO29942.1"
                     /translation="MPKRISSIYARAISLSTVIILAMAAFACSAQTGVPAQAKVEFYS
                     EAPAPMIEANAAILEDVATGQVLFSMNPDERLAPASITKVMTMLIVMEHAQEGKVSLD
                     DTVVVSREASLMGGSQVYLKEKEEVPIRELLAATAIRSANDASYALAEAIAGSADAFV
                     ELMNARAAELGMSNTHFANPEGLDDPGHYTTARDIAIMSRELLKHPEILELTSTWIGK
                     MRGGTYDLFNTNRLIKVYEGADGLKTGQTDTAGWCLVGTAVRGETRLLSVVLGAASDE
                     KRVGETSKLLDYGFQKFERTRFLDAGAPVGTIRVPSAARQDVTAVAAEDLVLLCPRGF
                     SSETEVHFEPLGQELSAPIKKGQPLGRAVAVMPSGEELGSTLAVAESDVPRANAVVRA
                     WRAVVRFFAELFGGRTRA"
     gene            26863..27852
                     /gene="ispE"
                     /locus_tag="PLU15_03105"
     CDS             26863..27852
                     /gene="ispE"
                     /locus_tag="PLU15_03105"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOO29943.1"
                     /translation="MRVVESKAWAKINLAFDVVGLRPDGYHDIETVMHRVELADEIRV
                     ERLEAGAVSEYEIRVDVFPQVAPAGTENIAYRAAMAFFEAAGERGAVSIGIGKKIPVA
                     AGLGGGSADAAAVLASMNRLWDVPLAEDELYHVARSVGADVPFCLMCHGDPPQAWAAM
                     ACGIGDDLTPLPPLPGVAVVLAKPSVSLLAGEMYGMWDARRARAMGGRGNARARALAN
                     WMRSQGGSALKGDAVSGSPVAQVVHLIGNELEPVAAERCPEVLEVKAALLELGAMAAV
                     MSGSGPTVFGLFESDDSAHSAARSLRKRAFSLSGRGPLDVIVTHAGPRIPPGS"
     gene            27919..28683
                     /locus_tag="PLU15_03110"
     CDS             27919..28683
                     /locus_tag="PLU15_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO29944.1"
                     /translation="MRVDVAILAGADNDGRLAEVSDAKYEAVVDINGKPMVEWVLDGV
                     LGAKSVDRVVVVGPSDALQPIVDRKNAGVRVQVVERRGNMIANLMAGLDEISDEKKAV
                     VIAGDAAFITSESIDGFVEMCERDPAEVHYAVVARDVIEEKFPGVKRTYIKLADATVT
                     GGNVFMVDAPVVRKNRGIIEKALDMRKNPLALGSLLGLPIILKYLFGRLSIADVEKRA
                     WDKLALVGRGVIVPYAEIGVDVDKPSDLELALQMLA"
     gene            28746..29126
                     /locus_tag="PLU15_03115"
     CDS             28746..29126
                     /locus_tag="PLU15_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOO29945.1"
                     /translation="MLKKVVDTDRAPAAIGPYSQGIVAGQFLFVSGQTPLDPATEKLV
                     DGGIKEQTKRALDNIVGILEAAGTGLDKVVKVTVYLEDMNDFAAMNEVYASYFGSEQP
                     ARCAIEVSRLPKDAKVEIDAIAIL"
     gene            29242..29499
                     /gene="spoVG"
                     /locus_tag="PLU15_03120"
     CDS             29242..29499
                     /gene="spoVG"
                     /locus_tag="PLU15_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOO29946.1"
                     /translation="MNITDVRVRKVNTAGKMRAIASITFDDAFVVRDIRVIEGQKGLF
                     VAMPSRKAPDGQFRDIAHPVTQEARDMIEDAIISVYNEQGD"
     gene            29963..30036
                     /locus_tag="PLU15_03125"
     tRNA            29963..30036
                     /locus_tag="PLU15_03125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29995..29997,aa:Gln,seq:ttg)
     assembly_gap    30101..30200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30418..30762)
                     /locus_tag="PLU15_03130"
     CDS             complement(30418..30762)
                     /locus_tag="PLU15_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOO29947.1"
                     /translation="MPKARRSNRTLVPQARQALDQMKYEVANELNIPTHLIEGDYWGN
                     LSARDCGSVGGNMVRRMIEAAQASLAATTMAGVKQGFQEVIPAQNLTAQEGNLSGPST
                     FPTSGNLPGTSF"
     gene            30952..32295
                     /gene="glmU"
                     /locus_tag="PLU15_03135"
     CDS             30952..32295
                     /gene="glmU"
                     /locus_tag="PLU15_03135"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOO29948.1"
                     /translation="MASFVGLVLAAGHGKRMKSALPKPLHPVAGIPMIDHVVGSLVRS
                     GASRVVVVVGHGGELIEEHLAGRAEIVWQREQLGTGHAVLAAGPAFDGYDGNILIVPA
                     DVPLLTSESLQQLVEEQSGSASDATILSMCVDDPTDYGRVVRDSKGNVAAIVEHCDAT
                     EEIRAIREVNTGVFCFDSRTLFNALPRISTDNKQGEYYLTDVIAIMISQGLSVRAVAL
                     KDASEGLGINSRVELSKAEQIMGDRKRREVMESGVTLIDPATTFIDASVEIGSDTVIM
                     PFTIIAGNSKIGGSCNIGPYVYIRDSEVGSGCNIGPFSYLRPGTSLADRVKVGDFVEV
                     KKSTVGEGSKIPHLSYIGDTVMGSHVNIGAGTITCNYDGETKHTTIIEDGAFVGANSN
                     LVAPVKVGSDAYIATGSTVTSHVPAGALAIARARQENKDGWVAKRRAARLSKEEK"
     gene            32298..33245
                     /locus_tag="PLU15_03140"
     CDS             32298..33245
                     /locus_tag="PLU15_03140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOO29949.1"
                     /translation="MDSCHRKLKIFACNSNPELSKAIAANLETDIVKAEVGRFSDGEI
                     SVQIFESIRGADVFVIQSLCAPVHDNIMELLIVIDALKRASARSITAVVPYYGYARQD
                     RKSQPREPITAKLLANLITAAGASRVLTIDLHAPQIQGFFDIPVDHLKAAPILAQHLE
                     SRGLKGCVIVSPDVGGVTRARGIAEHLDSTLAIVDKRRPRPNVSEVMNIIGDVRGKPA
                     VIVDDLVDTAGTLCQAADALIEAGATEVRACCTHGVLSGPAIERLANSQISELVITDT
                     IPLSKEKQIDKISILSVAPLFAEAIRRIYLELSVSILFD"
     gene            33267..33830
                     /gene="pth"
                     /locus_tag="PLU15_03145"
     CDS             33267..33830
                     /gene="pth"
                     /locus_tag="PLU15_03145"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOO29950.1"
                     /translation="MFIIAGLGNPGTQYECSRHNIGFMVADRLALELGAGKFRRAPHC
                     LSLVAKGTIAGTDTLIVKPQTYMNNSGIAVARATRQYGAGPESLIVVYDDMDLEVGRI
                     RIRAKGSAGGHKGMGSIIEHMGTENIARVRVGIGRPPPWEDSVHHVLGTFARDELPII
                     SDAVINAVSAILCIVEDGIETAQTRFN"
     gene            33882..37499
                     /gene="mfd"
                     /locus_tag="PLU15_03150"
     CDS             33882..37499
                     /gene="mfd"
                     /locus_tag="PLU15_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOO29951.1"
                     /translation="MTGIVDLLLDSTAFTEALRRVERGSRATHIVGLSGSQKSLFISG
                     LFCHARRTVFVVTATQHDAESLSGDIATMIGSDRVFVFPAVDVMPYEETPMSADLSAA
                     RAKAMAALARGEKCVVLAPARALGRRMVPVEIFRGAMFDIRVGQRCDLDGLVYMLAAS
                     GYEILGKVDGRGQASVRGGIVDVFPNDADRPVRIEFFGDEIESIRVFDPDTQLSQEML
                     DEATITPAREFMYDRERLERARMSIREELDRVGAGAAGKGRSVSADSAQAPSMLSPSS
                     MVMAHTRNQLRERVMHHLERFETGAYFDNDEQYLPFFYSERVMVADWAEDALFVLDEP
                     VRLKEALDGYESDIRETYATLLEADMVLPSQADSYMNAAEIIDAIKRRQIVSMSLLAR
                     APEGSCGAGPGGALAGYGDHIIAQTKQAEIFAGRIDDFIHEIKNFRKRGYRVVCMAST
                     EERCNRLAELLEGKGISPICASTLDVVPEAGTASVVRGELSSGFKCPDIKLLVLTEAE
                     IYGRQKKRRRFADVEEGARIASYTDLSPGDYVVHVNHGVGKYGGVQTLEAAGVQRDYL
                     LVQYAGEDKVYVPTDQIALLQKYIGMEDSPPRLNRLGGTEWTRTKAKARESVQDMAED
                     LLRLYAARESVKGYAFSPDTVWQQEFEDLFPYEETPDQWRAIVDVKRDMEKPMPMDRL
                     LCGDVGFGKTEVALRAAFKAAVDGKQVAVLVPTTILAQQHYNTFSERFHGFPMKVAIL
                     SRFQSEAEQKQVIRGLKMGSVDVVIGTHRLLSQDVKFRDLGLVVVDEEQRFGVAQKER
                     LKELRKEVDVLTLTATPIPRTLHMAMTGVRDMSLMETPPENRFPIRTYVVEKNDSLVR
                     EVILREIDRNGQVYFVHNRVHDIEEVAGRLRRLVPEASIGVAHGQMPEERLERVMLQF
                     MEHEFDILVCTVIIESGIDIPNVNTIIIDDADNFGLAQLYQLRGRVGRTNRVAYAYLL
                     FNRDAALSEIAEKRLAAIREFTSLGSGYKIAMRDLEIRGAGNILGPEQHGHIAAVGFE
                     MYCRLLEETIKELRGEKKEPEVPPTVIDLPVDAYVPDSYVPDSRQKIEVYKKINGIES
                     LEDASDISKEFIDRFGPLPPPVENLIAIAALKGLARDLGAVNVSSERQSVVVGFADGV
                     SYSASDLMRIARPFRGRVALAVGRTQRLRVKTHGMSEREILHTLIGILSEMKQGVAVN
                     Q"
     gene            37536..38093
                     /locus_tag="PLU15_03155"
     CDS             37536..38093
                     /locus_tag="PLU15_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HOO29952.1"
                     /translation="MRPTGIVRRIDELGRIVVPKELRRSLRIHEGDSVEIYVDPKGSV
                     VLKKYSPVGQLKVVATDMAESLSASSGSVALICDRDVVIAASGPLKEELIDRRVGRAV
                     EKAMAEKQALMVHFSGGGDASSILANDGPDSVAMISAAIAPIIADGDPVGAVVIGTTA
                     SDRTVGDLEESLAITAAGYLARQVE"
     gene            38136..39764
                     /locus_tag="PLU15_03160"
                     /pseudo
     CDS             38136..39764
                     /locus_tag="PLU15_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            39795..40685
                     /gene="mazG"
                     /locus_tag="PLU15_03165"
     CDS             39795..40685
                     /gene="mazG"
                     /locus_tag="PLU15_03165"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOO29953.1"
                     /translation="MADIEEIDRLQRLEAAQRTVKSDAASGAEVLGESQCRYPADKLV
                     EIMSRLRGEDGCPWDRKQTSSSLKPYVIEEAYEVVEAIDRQDPNLLCEELGDLLLQVV
                     FHCQIAREEGAFDFNDVVEAICAKLVRRHPHVFGDVKADDAGTVLRNWERIKQKEKES
                     SNGAPASILDEVPRYLPALMQAVKIQDKASRVGFDWECPEDAAAKVWEETEELREAMA
                     DGEMAKVEEELGDLLFAVVNVARLMKIDPEEALRRTNSKFASRFKFIEHRADENGVAL
                     EEMTLAEMDAIWDEAKEKGL"
     gene            40804..41115
                     /gene="yabP"
                     /locus_tag="PLU15_03170"
     CDS             40804..41115
                     /gene="yabP"
                     /locus_tag="PLU15_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOO29954.1"
                     /translation="MAAKGPDLSDEARRKRPGLQHRVVLVDRESMTVDGVANVESFDD
                     EEIILETNAGALTVTGHDLHIRQLNLEDGTIEIEGYVETCDYFDESPKKGRGLLGKLL
                     R"
     gene            41363..42247
                     /locus_tag="PLU15_03175"
     CDS             41363..42247
                     /locus_tag="PLU15_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOO29955.1"
                     /translation="MSRKPKRSAPKRRKPTTQKKSSMGLGLRIVTGILGVIISPVVFA
                     VSIFRPSMKMPKRWPISLTVLIIILAVYSWAGVKYVDQLSVAALDILGDYSSARLEVR
                     DMPFAETINFEGLRNGIDPCLVAAVISQESGFQTDAVSWRGARGLMQLMPDTWQEFMP
                     SAQCSGDHAPPACGNMCIYNPDANIHVGTAYLRYLIDRYDGDIIAALASYNAGMSAVD
                     DAQADLDSIQNDIPPYPETQGHVGNVVQAWTSFRSGLAEWRVRAVERAARARNVTSWI
                     AVGLLFLFMIWAAVRYPE"
     gene            42282..42791
                     /locus_tag="PLU15_03180"
     CDS             42282..42791
                     /locus_tag="PLU15_03180"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HOO29956.1"
                     /translation="MEGLNWQIRTVLASLAAGMLMGALYDLCAFFARVFRMQKGAMAL
                     VDLFYWLLSSAMTFSILMRVSRGEVRVYVLAGVAIGFWVYTLTGRLLAAPVFTIIEWT
                     ARRIFRSLRRAWNWARRPVSRTNRQISGVLMKGFSSTRRKLSQAAKFRRGAEDASEKR
                     GRAAREVNE"
     gene            42805..43131
                     /locus_tag="PLU15_03185"
     CDS             42805..43131
                     /locus_tag="PLU15_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOO29957.1"
                     /translation="MSSKLTVVLTIVLVVCVGLCYAGGRARLLALRKETECLEAECEE
                     WQSRIRDLESEIEEAQTDEYVERIAREKLGLVKPGETLYIVAEPDASGFTPVAKRKDR
                     PAEIGD"
     gene            43272..43670
                     /locus_tag="PLU15_03190"
     CDS             43272..43670
                     /locus_tag="PLU15_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO29958.1"
                     /translation="MSSIEVGSIVEGRVTGITGFGAFVDIGGGRTGLVHISEIADEYV
                     HDVNDYLKMNDAVRVKVISTDNGRIGLSIKQTDPNYQPRGGRRDRAYQQSFEDKLSKF
                     LKESDERHQDLKKHMDSKRGGRGTRLTRTM"
     gene            43823..45559
                     /locus_tag="PLU15_03195"
     CDS             43823..45559
                     /locus_tag="PLU15_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOO29959.1"
                     /translation="MSKKLGKSIEGGHGRTAEMREEHQDFVKPRAIDRAVVAAQLGRD
                     LIGSIARIPRRCSYGQPQVVSTYPVIWHNGGLGRVVKPKPFPTVYWLTCPHVREAISV
                     LESEGMIGEAAELIERDEDFRKAHECANTRYAAQRMALIGEDDLEFLEKEAPQMLRVL
                     RDTGIGGVARFAGVKCLHMHVADYMAGNNNPVGDMAVSRLRQAGVWLECDGGRCVPAR
                     VAAINAGSNSTKVLVADVVSRPNWSAGSDSSVCSKIMKAYGDSSVVGVPRVFGVCMDA
                     RITGLGHGLGETGRLSEAGRAATVEAISDFMGLSRSLGADRVWVTATAAARAAEDSEA
                     LIRQVKEACEVRLEVVSPEFEAELSFLGVVAGAGSAAAVGSVAPSVAIDPRSLLIVDS
                     GGMSTEFTRLDSCTGEVRSISLPLGAVSLTDEFLCSDPPSRGEIEQMRGRIRFCLEEA
                     REFVHGLPMDGEDGGILSTIVVVGGSAVTLASIGLELETLDPDMVHGYALHREELEEA
                     FLGLCSLACAERMQVKGMIQPERARVMPAGAAIILEVMDLAGAAEVVVSAAGILDGMA
                     ACIGLGRCGSKL"
     gene            45556..45735
                     /locus_tag="PLU15_03200"
     CDS             45556..45735
                     /locus_tag="PLU15_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29960.1"
                     /translation="MKSELGAISVFDGSAGGGFGNPLQATCVAQGARSWYTFLEVICD
                     NKVEAGVAERQTQGT"
     gene            45700..45785
                     /locus_tag="PLU15_03205"
     tRNA            45700..45785
                     /locus_tag="PLU15_03205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45733..45735,aa:Leu,seq:taa)
     gene            complement(46106..46477)
                     /locus_tag="PLU15_03210"
     CDS             complement(46106..46477)
                     /locus_tag="PLU15_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29961.1"
                     /translation="MEIREHAIIPLGYGKYVRSDKIIALDPIEDERGPGRRTRVYVDQ
                     LPGPLIASRTENSILASIVRTPTEVLEATAALELLQDLLDDIEQIGPMLRKSIKEEAG
                     LDLEELERKIREILTHELNAE"
BASE COUNT        10744 a        11127 c        14058 g        10735 t
ORIGIN      
        1 ggaccatggc gatggggccg aaccaccagg aatacagagg gcgttttaga gcttctccgg
       61 gaagcgggtg tagcaaaggg tgaataattg cttcctaaac gtgcgataac ttaaggtaga
      121 gcattggata cagaggctca gtttgacaat ttgccgcaca gtgtgtagta tgtgaaataa
      181 caaaactcca aaccagcttt tttcgtcccg tcaagcctaa gcaaggtgat ctgacgggcg
      241 ttacacatac actggatagg aggatttaac gtgctgccga ctaggctttt aagcgtgcag
      301 cggagtatgg ctctcatggc cgtagccctt attgtagcag ttccggggct tgcggctctg
      361 agcgaatcgg atgctgcaga cggaccgagt cgggttgtca catttcaccc ccgaacgggc
      421 tacacggaag atgtccttga aggagccgga gtttcaagac cagcgcgaac gtcagcagtc
      481 gcgtatgttg cgacaggttg ggtcacgaag cacattgtga ctaggaaagt gcccgtagaa
      541 aggccgatag agcgcattat cgatccgaag atggatcgcg gcgtcagtag ggttgtccat
      601 gagggatcgg atggcgagat gatgcaggca gtattggtca aaaggggcga gaacggggtc
      661 ataagcgaga ctgtcataag cgaaaggctt tggaccagac cgcaaccgaa agtgatagcg
      721 accggttctc gagaaccatt gagggttctg ttgacttccc gtggagacta cacttacaga
      781 aaggtcttga ccatgaccgc tactggctat gagccatctc cgagatcctg cggaaggtac
      841 gcggacggct acacggctat cggcatgaag gccgcgccgg gcgtggtagc ggtggatccg
      901 aaagtcatac ctctaagaac tagactatac gtagaggggt atgggcctgc agtggcggct
      961 gatgtagggt ccgccatcaa aggaaacagg atcgacctat tcttcgagac attggacgaa
     1021 gccctaagat acggacgcag acgggtgaat gtgtacatcc tggaagacag gtaaataaga
     1081 gtaaaaaacg ttgccggccc gtatgggccg gttttccaca tattcttcca ggctgtaatg
     1141 tggtagaata caagtatgag aggtttaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnng ctaaccctaa tggtgcaatt ggaggtcgcc cagaagctca cggctaagcc
     1321 tggttcgaga gcctatggcg agctttccgt gaagactcag tatttcactg aacccgtttt
     1381 gctctttgaa gtgcctccaa cagcgttcga tccgcctccc tcggttcgct ctgctgtggt
     1441 ccagatgact atgaggtcgg cgcctcctgt tgaggcagat gcggacatga tgttctccat
     1501 cgtgagcgcg gggtatggca aacgccgtaa aactctcagg aatgcactgc ttgcatccca
     1561 tttgtttgca ggagacagag atgctgttga agaagtcctt tcatgcgcgt ctgtcgatgg
     1621 aggaaggcgt gcagagaccc tctccttaga ggaatttgcg gcgatatcgc gtgcatacgt
     1681 cgcatacagg gcaggtgaaa acggttgaaa tacttcataa gtcctactaa aggacgcctt
     1741 acattcgaag agacattcga tgacatcatg gcgtttgtcc atgacatgcc ggatgaagaa
     1801 tacaagctga tcattggaac ggattcccag gtgaacggtg acacctgctt tgtcagcgcg
     1861 atagtgatcc acagggtcgg acgtggggcc cgctattact actgtcgcga gcacgagact
     1921 attggcacag gcctcagaca acggatcttt tatgaggcgt caaagagcct cgaccttgcc
     1981 agccaggttg cccacaagct tgcggaaaac ggatatgccg atgtcaacat cgagatacat
     2041 ttggatgttg gcgttaatgg caagacgaag gagcttatcc gcgacatagt cgggatgatc
     2101 agcggaaccg ggttttccgc ccgtataaag ccagattcct atggtgcctc gaaggtggcc
     2161 gacaagcaca cccggtaggc tgctttccga tccgcgtcca tgtccccatg ttttggcaca
     2221 ctctctgaac agccaaggct gtatcagcgc tcttccatgt tctccaaaac cttcgattat
     2281 gcctggggat tgtatgcttg caagcgctaa actctgtgca tctcggcacg tttctcctta
     2341 cccttatccc acatatcctg aacgggaact gaagatcgtt gtttaaacag ggtacgctta
     2401 tggcacgctt ttgcgatggg cgaggtccat agcgaagatt cgatcagttc ctggaaaagg
     2461 agggaagggc ccttggctaa cagaaacaga attcgagaag gcgacatagt cacccggaag
     2521 tcatacgggg gcgacgtctt ctttcaggta attcgggttg actcatatgc cgtgggcgat
     2581 gcaaccgcaa ccctgaaagg gttgaatatg cgcctccttg cagacgctcc aatatcggat
     2641 ttgtctattg tagaagaacg tcaactactc gatttcaagc ggaaggtgat gcgtgaatcg
     2701 gcagaacatc tcagaacggt gcgtgtgcgg aggataaccg aggaacaggc gagatacaag
     2761 aaggaacgca ggcgatcgag agggtcagct gctttggccg aggatatctt tgaccttccg
     2821 ggcagggtgc ttcatataga tggggatgcc gattacctta gggattgcat gaaatactat
     2881 aaggaactag gagttcctgt cgtgggagaa catgttcccc ctcaagatca gactgcggcc
     2941 gttggtccat tgctcgatga gcattcacct gatgtgctgg ttcttactgg ccacgatgct
     3001 cttaaaaaaa agaattctga tcgcgcttca atgagcagtt actggaattc ggaagcatac
     3061 gtgaacgcag ttcgtgtggc cagacaacac gagatggaca aagatggcct tgtgatcatt
     3121 gcaggggcat gccagtcgtt ttatgaggcc ttgatggagg cgggtgccaa cttcgcaagt
     3181 tcacccggga gggtgttgat ccactgcctc gatccagtcc tattggcgga gaaagtggtg
     3241 aacacgcctg tggaggagat catcaggata gaagaggcca tagaaaatac aataaccaag
     3301 cggcctggcg tgggtgggat tcagactagg gggaaaatgc gcacgagcat gcccaagacg
     3361 cccatggggg tttttggggc aggcgcgaac agggacaatc aatcagctag aaactacatg
     3421 ccttgacgtt tgcacggaat agccagagga atctaacgtt gactgatgtg agtcaggcag
     3481 cgcatgagga agctccagag aaaatctatg tctggagctt gcttgttcgc ccggcaagtc
     3541 tgttgagtcg actgactgga agaagtcccg ctacccaacg ggtgtacatc catgcaaggc
     3601 ctcacggaat cggcatcatc tttaccttgt gcccgccgac gccaaagcaa agggctttac
     3661 atgctcgaga aaggcaaggg tgtgatttac agagatgctt tgcgcaacga acttggacgt
     3721 atcggaacag cgaggacagg tgcaacaggg agctgagcca aaccgctctt agtagcggat
     3781 aaacttcgca gcatcttttt cgagctcggg gtaagagagg ttttggcaac tgcgcccctg
     3841 atcgccggca tgtcccattg cagccccaac agcttcataa tactgatttc cagctacgtt
     3901 caatttcgta gccacaaaac ggaccttgac attaagccaa gccgtttgat atactatttc
     3961 actttacttg actttatacc tcatccgtga tagaatgaca aaagctgagg cttgaaagga
     4021 aggtgaaggc gttggcgggc aataatgtcc tggacaggat cagagaagat cttgattctt
     4081 ttgtaggcaa gcctgttaag cttcgcgcga accagggccg caaaaaaatc cttgaggccg
     4141 aaggcgtatt ggaacaaact tatcctaggg ttttcgttgt gcgtatagat gaggacccgc
     4201 ggagcgtgaa acgggtatca tatacttacg ctgatgtgct cacatctacc gtcgaagtaa
     4261 gggttaagaa caagggcatg gaaaccagga tagggtgtgc aggatcataa ctgcctttgc
     4321 tgatcacgtc taggctgacg tttgaacgca agaatgctgc acatgtcctg ataagggcag
     4381 aaaacgcgca tgccggctca cctgttggtg tctccgctcc ggtaggggcg tgccggtttc
     4441 aatgataacc tcctttccgt gtatttatgg aaggaggttt gctctttttt cacggaaatc
     4501 gctcctgtgg aatatagata gtctgcggta tacagcgagt ctgctgcaga ggagcgattg
     4561 aggtgaccca cgaatatcgt ggcgtaatcc acgtccatag cactttttcg gatggacgcg
     4621 gcgacatcga ccagatcatg ctggaggcaa atcatgccga tgtcgacttc ctgattctta
     4681 ccgaccataa cactatagaa gcaaagctca ggggatacga agggtggcat gactgttgcc
     4741 tcctcgtcgt tggcgaggaa attactccca agacaaaagc cgaccattac ctggcccttg
     4801 atatcccctg cgccattgat ccatccgatg acccggctaa gaacattgca gcagttcgga
     4861 aagcgggggg tatcggcatc attgcccatc ctacgggcgg tgctgtcgtg aagggcgagt
     4921 atttagagta cccgtggacg gattgggacg caggcccgtt tgacggaatc gagatatgga
     4981 accacaccta tgacattacg ggaaaagcaa agaacatctt tgagctcatt ttctacgttc
     5041 tgtttccaaa ggccgcaaaa gccggaccaa tacaggacgt ccttgataca tgggaccgct
     5101 acagccttag ccaatctcgg aggatcatcg ggataggagg aactgatagc catggattgc
     5161 tcagccgcta ctctgatgat ttcaaaacgg tctgcacgta cattctagca aaagaggcct
     5221 tcaactatga cgctgcgcac gacatcgccc tagtgtatga ggcgcttcgg gccggccgtt
     5281 gcttcgtggg ggcggacaag gtggcgaagg ccagcggatt caaatgtgag gtagtgtgcg
     5341 gcgacaatgc tgttcagatg ggcgggaggg cgtgcctttc tcgtggagcc tgcctcaatg
     5401 ttcatgcgcc agagccgggg ctgatccgca tcatccataa cggcgatgcg attttggaag
     5461 aaaagggcgt ggacctcgag gcggcattgg aatcgcctgg agtatatcgt gtcgaagtca
     5521 agcgtcgcgt ttatgggaga tacgtgccat ggatatatgg caaccacttt ttcctcgcat
     5581 gagcaccaca aaggttcaag ccaggctgac gtgcctggga tttgaccccg gcccctgcga
     5641 cggaaagtat gggcccttta ccagggacgc ggtgcgccaa tgccaggcag cctacaggct
     5701 cgtggccgac ggcgtcctgg ggcccgagat taaaaggctg ctcagccagg atttgccttc
     5761 atgccgggtt acggtgaggg ctgtagaagg ccagtcccta tcgggtgtag ctcgagctct
     5821 gggaaccacc gttgaggctt tggtagaggg caaccggcgc aaacgctatg aagatgtgtt
     5881 tccaggcgaa aggctcatgg ttcacaaacg agctgtaggc gcgttcatgt atcctttggc
     5941 tggtgccgag gggggcactg tggatctgga catcactttc gaactgccgt ggacttttgc
     6001 cctattcccc atgttcgcca tcgccgacac cggcgaggtg cgctcggtta tgagtccttc
     6061 gccgctcgcg gtgggcctta ccgaacgaga aaatggccgc gtggactcta tcagggtatc
     6121 caggtctttg cgcgggagga agcagggggc agagccttta gctattccag ttgccaagaa
     6181 aagtcacgca gattatggat cgcccgtagg atcatccgtt cagtttctac ccggagcccg
     6241 tgagtctgca gccgccgcga agacggtgcg gcgccccggg ggcactcagt tctttgccat
     6301 gctttcgggc cccgctgata tcaactgtgg aaccttggca ttgctcagaa gcggccgggc
     6361 ggccatagtt tccaccactt gctccattgc cagggaagca gccgcatcgg gtgcggcggg
     6421 ggtagccgta aacatccctg ggctcggcga cgatgagtgt tccatgtatt cgcggtttgt
     6481 tcgttgtctt tcgcgggaat gccacaggct agggatgaag cttgcggtaa tcatccctgc
     6541 gccgtcttgg caggctgatg gccaagtcca ttttcagggc tcttctgcgc ccgaaaggcg
     6601 cttgccgtgg cctaatggat acgatgtaga gctgctcggg tccctcgcag atctggtatt
     6661 gctcgatatt gcggacccat ctgcgttttg ggacagtatt gtgttggcat gtaggcatgt
     6721 ccccagatgg aaactggcgg ctgtgctgga gcttcgggca tatcggcagg aaccggacgg
     6781 acatgtgccc atgagccaag aggagctaag ggcgttcatg gccagacacg tagtgagagg
     6841 cggaagggat gagtttagcc gggtcccgta tctatcctac cgcgctcgag gcgttgtgag
     6901 gcgcatatgg caggaggatg cagagagcct cgggaaaaaa ctgcacgcag tcaacaggct
     6961 taacatactg ggcgtcgttt tcaagggcgc ccgggatgcc agggagcaag tccttgatga
     7021 ggtgtgccgg aggttcatta tcatgtgaca tgtagcatat actctggcag agggcggact
     7081 gacccgccat cgccaggggg gaggaagaag tgggaattca gctcgagcac aggcagctct
     7141 cgattacgag ggtagctgcg caaggcatgg ctgagatcag cctacagcga tctgtagcat
     7201 tacttcagga ccatccggac atatctgatg attctgccca ttcgatcctg tgggcccagg
     7261 gcgtgccgat tgtggcctca gtggaaccca tggacgggca gatcaaagtc acaggctacg
     7321 tgcaatccac cgtgttctat gtggccaaag gcgccccgga ttttgtcggg aaggccgtca
     7381 tgtccgagcc gaggtttgaa gcggtgatac cggcgccggg agccaccgtc tcggacaggg
     7441 ttaaagtgga tcttacccta tcgcagttgg aggtggaaac caccggcaca cgcgggctcc
     7501 tcctggctgc cactatcatg gcctcagcaa gagctttgcg ggacgaggtt gttgaggccg
     7561 ctgtgtccgc agaggctaca ggcgggtcac gcatatctgt ccattccgaa gagataaatg
     7621 taaccagcat acatgagtcg ttttcggaga aggtcgagtt ttccgagatc cttagtatcc
     7681 cagatgaaaa agcggcttgc ctggggagcg aatcggcatg tggcgcggca ggaactgcca
     7741 gagtaatcgg gatacaggtt ggagacggga aggtaactct tgacgctgag atttctgtgg
     7801 aaattgcttc gaccgttgtc agaggggttc cggctctcaa catactccga tttgagcgcg
     7861 tccccataca tgcgtctttc gatgttcccg ctgctgagaa gggaatgact gccctaaccc
     7921 agctggcgct ctcccatgtt tccgcgatcc tcctttccga tgctgaagtg agtatcgatg
     7981 gcatcctcga tgtagacgtg gacttggtct catcagaacg tctgtcggtc atcgttgaca
     8041 tcgactctga aacccaggag atagtggata tcgatacggc ggggattaca gtggagaagc
     8101 ttgtgggaga gggcatgatc gatctagatc tgcacgattc ggttgcgctt caagcgctca
     8161 tgaggcgata ttcgttcacc ggaatggagg atgttgaagg cagcatcgga ttactggggc
     8221 tgtccgatat agagaccacc gatgatgagg tttcgctgag gggacttctc actgccaggg
     8281 caatcttgag cgacgtggac gaggatgaag agggacaatt cccgatcaac ttcgcgctcg
     8341 atatccccat agagttttcg gattctgcgg aagttccggg ggtcagagcg ggagacaggg
     8401 cggagccaag gtcctcgaca cacaccgtgg tagtgggaaa atcggcgcca aacaggttgg
     8461 atgtggaggg ctcgctccgt gtagacgtgg aagtctatcg acctgtcgaa ctgcaagtgg
     8521 tgacttcggc cgagaccatt agtcctgtga cccttgatcc ttacgcgatg actttctacg
     8581 tggttggacc cggagatacc cttgcccgga tagcaaggag atacggggtt cctccagacc
     8641 gtatagcggc tgcaaatgga atgtcagtta ctgaggaggt tactcctggg cagaaggtct
     8701 atatccccac cagacggtag aagcgcacgt ccgccgctga gggcctcgat gccccgacga
     8761 tgcaatacat caatgcatcg aagaagggga agaggccctc agcctattgc aggtccggta
     8821 tgcccgcgaa tcggacggtg gctctgatgt cctttgcaat ggcgcatata ctctgaaatt
     8881 tgcgtgctaa aagcgagctg gaggaatcgg ggctgatgta gaagaagaat actatcgcct
     8941 gcagaacagg ttacacgcaa ggcctgggcg tgatacttcc aggcgtctga atggtgctat
     9001 atagcccgct gacgaacgtg cagaattcga cggccggcta tgcgtgggat atggagggac
     9061 aggctttgga cgcaagcaag tttatcaact cagtgatgtc cgagtatgat tatcgagacc
     9121 aggtggtgca cgtagagacc attccgccga gggaggctcg ctatgctcag ttgtcgccgc
     9181 ctgttgcgcc tcccatatcc tccagcctga aggagatggg catatcggag ctttataccc
     9241 atcaggctga ggcagtctcc cgcgccaggg cgaaacagga catagtagtg gtcacgtcta
     9301 cagcaagcgg gaagactctt tgctacaata tcccggtcat agaggctctt atggcagata
     9361 gcagcgcaac agccctgtac atctttccca ccaaagcctt ggcgcaggac cagcagaggg
     9421 ggcttgtgcg ctttgtagag cttgaacccg atcttcccat caggtcggga acatatgatg
     9481 gcgacactcc ggattctaca aggcgcaaac tgcgggagaa tgccaacgtg atcctcacaa
     9541 accccgacat gctgcatcaa ggcattcttc cgtcccatcc aagttggcgt cggttctttg
     9601 cgggcctgcg atatgtggtc atagacgaga ttcatgcata caggggcgtg tttggatcca
     9661 acgtggccaa tgtagtgcat aggcttcgca gagtgtgcgc tcattacggg tctgatccta
     9721 cgtttatatg ctgttctgcg actatagcca accccggcga gctcgcaggc gcgatctccg
     9781 gcaaggatgt tgtggtgatc gacaatgatg gggcaccaag ggggccgcgg aagttcgtgt
     9841 tttggaatcc gccgcggctg gaaaacacaa ttcagcggcg aagttccaat tcagaggctg
     9901 agcggatcat ggtgaaactg attgagtcga aagttcccac gataaccttt gtaagggcgc
     9961 gggtcgtcgc cgagctgata tacaagtacg ccgttgagag tctgcagcgc aactcgccgt
    10021 cgcttgcgaa gaagatcaag ccatacaggg gaggatacct tcctagtgaa cgaagggaga
    10081 tcgaaaggca gcttttctcg ggagaactgt tgggagtggt gagcactaat gctctggaac
    10141 ttgggatcga tatcggttcc ctcgacgcga gcatcatagt aggctatccc ggaacgatag
    10201 cgtccacatg gcagcaggct gggcgcgctg ggcgaaaggg cgaagaatca ctctcggtat
    10261 tcatcgccca cgatctgcct atcgaccaat accttatggg gcacccggaa tactttttcg
    10321 accgttctcc cgagcatgca gtcgttaatc ccgcaaaccc ctacattctc gtgggccatc
    10381 ttcgtgccgc ggcgatggaa ctccctatca gtccgctgga acttgcggat tttggagagt
    10441 atgcgccagc cctcatagag ctgcttgaag agaagggaga ggttctcagg actagactgg
    10501 gctggcgttg gattggccgg ggctttccgg ccaatgatgt gaagctccgt aatatgtcgg
    10561 agaatactta caccatagtg gatacctcat ctgaccatgg gaaccgggtt atcggcacca
    10621 tcgacgagct gagcgcattc gaacaagtcc acccggaggc cgtgtacatg catgaaggtg
    10681 aaacatatct agtgtcagac ctcaatcttg tggagaaggt ggcctacatc cacaaggcgg
    10741 atgtggatta tttcacccaa tcggtgacgg agacgcgcgt ccagatcgag gagaaagagc
    10801 agaataagat gtggaggaag agcgaagtcg agttcggtga cgtaacggtc accaatctga
    10861 catatatgtt tcggaagatc aagttctacg agcgcgacag tatagggtat ggcaaggtca
    10921 acttgccaga gaacaacctt cccaccactg cggcatggat catgctccct gaaagtgccg
    10981 cgaagctcgt ggcgagcctc ggcagaattc cggcggaggg cctaatagga atagggaatg
    11041 ctgcatctgc tgttatccct ctatttgcaa tgtgcgattc catggacata ggcacggcgg
    11101 tggattcctc gaacacgggc gtgcccacgt tgttcatata cgataggcac cagggtggag
    11161 tggggtttgc gcagaaggcc tacaagatga ttgacgaggt tatggaagcc tgcctaaacc
    11221 tgatacagaa ttgcccgtgt gaagatggat gtccttcgtg tgtaggttca cccataccac
    11281 cgtatgccca aaacgatccg gatgcaacat cgagagggcg cattcccgac aaagatacgg
    11341 ccctcatcat cctccataac ttgctcgaga aagagccgta cgtcccggca ggtccgccaa
    11401 aatgggccga aggctccgat aacgcggatg gcgcctgcgc gggctacagc gaggaagact
    11461 tcagaataga tgttaagccc ttgcccgacg gtgtcgagcg gcggatcagg aagatgatca
    11521 acagcagatg acgcccgagg ttggccttga aggctcgatc atgtagttgg tttgccatga
    11581 tatcttaggc gacgaggatg agccggttga atcgggcaac tcgaatatgt tgcacgcgtt
    11641 gtgcggataa cggacacaag atctgacgaa ttcatgaaac gacactcccc atgcccatct
    11701 cctgccacaa ttgatccgaa agagcgatgc ttatgcaaga cccgaatatt ataaatatac
    11761 ctcaaataac tacgaaaaac tgaaggcgga tgctgcatcg atcggctgat gtgaaaacga
    11821 gataacccac atatacgtgg ggatattacg ctcattacga atcgggcgcc ctgaaattct
    11881 gacgcgtgct attgacaata ggcctccgag cagtgtatac tcatgcagca gatacgaatt
    11941 atggtggagg agaggtcagc tgtcatgaaa agactgttag cccttgcatg tttatgttcg
    12001 ttggttgtgg tggcatttgc agcacctggt gttggagcgg ccaagccagt tctcaaggtg
    12061 ggaactagcg ccgacttccc gccttttgag tttcaggacg agaagaccgg ggcatatctg
    12121 ggattcgatc tcgatctgat cagggcgatt ggggacgcga tcggaatgga cgttcagatt
    12181 gtgaacactg cttgggacgg cttgatacct ggacttatca ctgggaatta cgattgcatt
    12241 gtctcagcaa tgaccattac tgacgagcgt ctcaaggccg tcaacttttc ggacccgtat
    12301 ttcagcgccg ggcaggttgt agtcaccatg gccaaggata ccaagatatc gtcaattgcc
    12361 gaccttgagg gcaagaagat atcggttcag ataggaacca ctggggattt ggtggcatca
    12421 gagattaagg gggcaacagt gaagcggttc aacctggctc ctgatgcagt tcaggaagtg
    12481 aggaatggcg cggcccatgc ctgcataatc gatttggcag tggccgctga gattgccaag
    12541 gaatacaaag acataaagta tggcgcgcct ttcacaatgg aatactacgg aatcgccatc
    12601 aagaaaaaca ataccgcttt gcttaaaaac attaatcggg gattggcaat gatcaaggct
    12661 tccggaaagt acgacgagat atacaacaag tggtttgccg gcaactagtg gcaggctaat
    12721 gccttgcgtg gaagatattg aggccgtgcg cgcttggaga gatgacctct ccagcagctc
    12781 acggcccatt ttaggcgcat ttggttctca agcgatagat tagaagatga cactttgggg
    12841 gagttgctgt ggctctcgat ttttctataa taccgggtat catgcctgcc ttgctggccg
    12901 gagccaaaat gaccatagag cttgcagtga tatcagttgc gatcggattt gttataggct
    12961 cattagttgg aatagcgcgg gtcagtcaga acagggcgct cagggttatt gcaggcatat
    13021 acgtagactt cattcgaggg acgcctctct tggtccagct gttcctcgtc tattttggat
    13081 tgccgacact cataggtcgg cccgtgcccc ccttcatagc tgcgatatcg gccatgggca
    13141 taaacagcgg tgcatacgtg gctgagatag tgagggcagg aatacagtcg atagacagag
    13201 ggcagacaga ggcggccaga tctttaggac tgaccagttc gcagacgatg caatacatca
    13261 ttttcccaca agcgtttcga cggataatcc ccccattggg gaacgaattc attgcaatgc
    13321 tgaaggactc ttcgcttgta tccgtgattg cactagaaga tcttcttcga aaagggcagg
    13381 ttgtcattac cagaacattc aggccgttcg aggtttatac cgtagtggct ctcatatacc
    13441 tcgtcatgac atttgtcatt tccaagcttg tcagctggtc agaaagaaaa ctgaaggcgg
    13501 ttgggcaaat tgattaaaac tgtcgggctc agaaaatcat tcggacataa cgaggtgctg
    13561 catgggattg acgcaacaat taacccacac gaagtggtgg ttgttgtggg gccgtcaggt
    13621 tccggcaaga gcacctttct gcgttgtctc aacctgttgg aaaagccgac cgcaggggag
    13681 atatatttcg acggccagct cataaccgca cccggagtga atgtcaacga gattcgccgg
    13741 gagatgggga tggtgttcca aagctttaac ctcttcccgc acctcaccgt gcttgacaac
    13801 atcgcgcttg cccctaaaat agtgaggcat gtctcgaaag acgaggcgga agagacagct
    13861 cgcgatctgc ttcgcaaggt ggggatcgcc gacaaggagt ctgcctatcc gcaggagctg
    13921 tcaggcggac agcagcagcg cgtggctatt gcccgcgccc ttgctatgaa acccatggct
    13981 atgctctttg acgagcccac gtctgcgcta gacccagaga tgataggaga ggtcctagat
    14041 gttatgaaag ccctcgccgt agaaggtatg accatggttg tggtcagcca tgaaatgggt
    14101 tttgctcgag aagtggcgga cagggttctt ttcatggatg ccgggttgat cgtggaagaa
    14161 ggaccacccg ctcaggtgct gggcgatccg aagaatccga ggaccaaagc gttcctggga
    14221 aaagtattgt aggagtgctt tgctctttcg ggggtttgtg aggtacgggc catgccattt
    14281 tttctcctac gacaggggta aacgcaggag gaaaagaggg catggcccat tgaatttcca
    14341 ctgctttgcg actgcaggct gcaatccaac cgatcgcttg gcacttgggt ccgatatgga
    14401 tccaagccgg ggccatgcgt tcagccacgg ccccggtaac ggcggtgaat tatctgcctg
    14461 ccgatgttgg gccgaaggcc attgtcagag cttcagtgaa attgccgtgt aggttctttt
    14521 ccaaagtccc cggaactgcc agatgtagcc tggtgcttct tccttcccca tccctggccg
    14581 ggtgcattcc aacgcagacc ccatctttag ctgcagcctt tcacgtgatg caaggataaa
    14641 aacgtggtac acgggcacat atctccctat ccggatttcg atggagcaca tcgatacttg
    14701 agattgacat tactcaagcc gtgaaggcct taatgtcggg ggcatcctcg aagagtgtgg
    14761 cgacagcgac tgccaaggtc tgcatagtca aagcgggaag ggggcgcccc tagtgttgca
    14821 gggtcagctg tgccgggaag tcgctgatgg ttgtgttggg ctcatagtga gacaggacta
    14881 ctgatgtgca tgccttgtgg ggcaggtgtt gtatgctctt tctgctttcg tgtggcccaa
    14941 catgaaggac gggccacgcc atccttcagt gaaatatgag gcatctatgg caggataaat
    15001 agtatggtat gtcaaagcac tgggaaagtg atacaatccg gacattgtag gtattgacca
    15061 atgctacagg gaggttattg aatgaaggga aggcatttcg gttcgcgcct cagcttgcta
    15121 gctgttattt gcgcgttttg cctgacgacg agcgggtgca atatgggaga ttacaccttc
    15181 gtagaccttc ctgtcagggt tgcttgggag ccagatcagc ttatcagagt catcgactcg
    15241 gaaaaagtat tcggtcacag gttggggagg ggcgacgggc tgagagtgtg gctcgacctt
    15301 ggtcctctgc cctgtgatct tgagccagag tccattgaaa gagccacatt gaagttgccc
    15361 tgccaatcct tttcgagagt ggcaggtgcc gtcacatttg agatatacat ggtcaaatgg
    15421 acgtatgatt cgggaatggg cgaatggccc gacattcctg agttcttaca ctccagcgaa
    15481 cttcctaacg atgtacaacc catctccagc gccaggcttt ctcatgacgt agggtgggag
    15541 acgcaacatt cgaacggata tgtctatagc tcccaataca tagatactga agtcaagtat
    15601 ctcagtttcg atattaccga agcgataaag gcgtgggttt ccggcactcc caatgcgggc
    15661 cttaggatta agcagaagtc tccggcgcgc ccggatggac ctgtatggag tctcttgatg
    15721 gccccggata aacgtttgaa ggatattcat aaggcattag atgagcttcc gaagagtgcg
    15781 aatggcccgt ctgaccagcc gatagcaata gtgtgcataa agaaagccgg agaagcttcc
    15841 tgaatcacag agcacctagt tatgtccgat ataatgctgt aaaaaaggcc agagacagtt
    15901 gtaatagtgg cctagcctcc gttcgtacac attttagcgg agggatctgc aatggaacaa
    15961 gcgaaacggg ttttcgtgag gactaagact gccgacaggg cttcgggtgc taccgaacgg
    16021 gcgagctcgg aagggcacaa gcggagatgg aagtgcgatt gggctgcgcc tcgaaatagg
    16081 ctgaaaagtt gtccccgagg catgaggctt tcccagcgcc ctgatagcgg aaagggagtc
    16141 atcctcacat tggccagaat aggcgcttgg tttgtgctga tcgggttgat acttggagtt
    16201 gtatcaggcg ggctatttgt ggggcccttc ggaggctata tcaagaagcc accgcgtgcg
    16261 cccctcgatt cggagcacgg cgatggctta ctccctgtaa agccctcccc gggttatcgt
    16321 gcgcctaatt tcgtaggcag gctgcctgat ggaactataa tatccctcga cgattgcgca
    16381 ggcatgccca taatactagt cttcttcaga atcacgtctg ccccgtccat tgcccagatg
    16441 ccggctgtgg accgacttta ccgggagcgg caggaccgat gttgcgtcat agcagtcgcc
    16501 gtcttcgaaa gggaggatga cgtaggccgc tacatggcag caaacgatct cgacattccc
    16561 atgatcgccg atatccgagg cgaaatagct caagcctacg acatcaccgc gcttccgacc
    16621 actttcatca tagatgggca gaggataata aaggatctca gaataggcgc aatgacctat
    16681 gcggagttgg aacgagccct caagcctctt tactgaacat ggctttgaac actaggggtg
    16741 ctccatatgt gccccacagc gccatcacag cgtagcgcag catctgccag aaagaaccct
    16801 cgggtaatat cgctttgaga ccgtaacgta tccccattag aactgccagg ccaaatacga
    16861 ctttgatcac ttgaatgaaa aaactcgccc ttacatcgta cttcacatat tccttttcga
    16921 gagcgtatcc aactccgagg cccatggtga atccaagaag gcttgcggac tcgcgggcca
    16981 gttcgaagct gagggatgag agggcgaaga tcacgcctgg aattactata gacgcggcga
    17041 cagcggtgtt gacagacgtc ttcttctccc gattggccaa ggactcctcg acgagcatgt
    17101 aaacctgaac aatgatgagt cccagagccg tgcctacaag tacgtctttg gggtagtgca
    17161 ctgttgcata gatccgtgtg agacccacta ctgcgactac aatgacgctg agcgcggtga
    17221 accaggcgcg cttgacatgc caggcagagt aagcccagaa ggtggacgtt ccctgcgcgt
    17281 ggccgcttgg gaaagaccat ccggttccgg tgtcgagcag gaagttcggg gcatcgagat
    17341 gagggtatgc aacataaggt cgaggcactt tgaagacaaa cttgaggccc acgttgatac
    17401 acgaggagat ggaggtcacc atgcccaagt tgaatcctac agacttgtca atacaccagt
    17461 agataatggg tatcgcgatc atgtagaagg aaatggagcc cagatcggtt atgatcttta
    17521 ctaccctgtc gaggccggga gttgcgattc tcctaaagaa ttcaaaaact agagagcctg
    17581 tcaagattga ccacctccgg ctcagaattt cgccaagggg gagccgaatc ctctgagatg
    17641 tgcatccata ggatgtgtcc caacatatgt gtggcggaag gttcacatga atggtaaaat
    17701 atagttgcaa atgcttctag ggatctgcca atgagggcct tatctttccg cacacggttt
    17761 gacgacgaga aaattggcgc cataagcgca acgtgaaccg aattctatgg accattaggg
    17821 tattcgcgac ctttgctgtc tgacgcgcgg acttctccat gcgatgttgc aagacatgga
    17881 ttgtccaata ctccatctcc cgtttgcaat cgtgtgcacc acatgcgccc tccttgggcc
    17941 gatattttcc aggcaggata cttggttcgt ttgtgcgaaa catcgtctca agggagatca
    18001 ggttttgctt gatctgccgt atcatttgat atggcttgaa agtctggcag aacaagcgga
    18061 tgccgtatgc cgggcgggat tttaatggac aagccattcc tggtattcca agggtttttt
    18121 gtctacgaat acgggtgcgg gctttatggg ggaaatgcca acaggtgatt ggaataggcc
    18181 gtacgaaagg acgaatgagc actcttatgc aagagtttat gagaaacact gaaaaaagat
    18241 acggtttgga ggaaaatgca cttgatactt agcctcggcg atgttatgat agacgcatat
    18301 tttttcacac ccaattcgcg ctacccgcgc accgacactg ttggctgcgt tgagcttgcg
    18361 gctggcggat cggctgcaaa tttcgccgtc tgggtggcgc gctatggcaa cgcatccggg
    18421 cttataggcc gtgtgggcga tgatttcctc ggccatgcat tggtgaagga gttggaatct
    18481 gaaggagtcc aacccttcct tgtattcgac ccagagtgtg ccacagggcg agttggggta
    18541 attatctcgg aggacggcga acgcgacatg atctgcgata ggcgtgctaa tacccgcttg
    18601 tctcctgatg acatcccaga ggaggttatc aggcaggcgg aatggctgca catatcggga
    18661 tacgcgttct tcgagcctgg tccggccaga gcggcccgtc gggccatgga aacgtcttta
    18721 gcggcaggca ttcccgtatc agtggaccca gctgcctatt cctttatcaa tgccgtagga
    18781 aggagtgatt tcctcaaact atgtgccggc gcgacggtca tcttgccaaa cctagatgag
    18841 ggtcgtgcca tgaccggatt ctctgatccg ctagatattg ccaacagcct tgtagagtac
    18901 ttccctgtgg tcgctctcaa gctcggagcg gacggctgtc ttggcgtatc acggcatgaa
    18961 cctgagccga ttcggctgcc tccgatcccc actgaagtgg tggacactac cggcgccggc
    19021 gatgcttttg gagcgggttt tgtgatggcc tactcccatg gtgagggact tgccgccagt
    19081 cttgcccgcg gcaaccaact cggcgcgaca gctgtctccc gtgtgggtgc tcgaagtcgc
    19141 gaggccctgg atctagctta gaagtattct ggctcctaca gtaagcagcg taagtcctag
    19201 tattttgatg gggctgaagg cgattggcct gacgccgaac caacccatgt ggtctatgat
    19261 cgcggccatc ccgatttggc ctactatgat cgctgttgtg gcgtttacgg cgccgagctt
    19321 tcggattccg taagccactg cgattgtgat taatggtcca acgggtccgc caaggaggct
    19381 ccaccatggg gcagcgccta atcgcgcaag gcctccactg ttgagccctg ccgctagtcc
    19441 tgctccggcg aggagcgctc cggaggccaa gaccacgaaa gtagtctccc aaagtccaat
    19501 tgtttttgca gcagtagaat tcacgacgcc ttgaaaagcc attgctattc cgctccccgc
    19561 ggctatggca agggctggat tgaagatacc cattgtaaca ccagctttcc agtatgtgtg
    19621 ccaatagtat ggccctaagc acctagtctg tgtaaataag ggtccgctat tacataagga
    19681 tagctggcag gattatcact tgttagagcg aatactgcat gtaataacca tgtcagggga
    19741 acgggtatcc cggcttattc ccggaactgg aatacctgcc cggaagccaa aaaatgaggg
    19801 aggtcgtttc gtatgcggat tcgtaagtcc ctactgtggt ttgcttgctt catgactgtc
    19861 ctgctgattg cctccactgg ctttgcagca gagaaactgg ccaagaagca gatcctcagc
    19921 ttcaatgtgg gaacagaacc gtcgacgctt gacccggcaa agtccactgg aatcccggag
    19981 gccattatcg agctcaactg ctttgagggc ctcacgagaa tggacgagaa caacgtgcct
    20041 cagccagcta tcgcggagag ctggacgatc agcaaggatg gccttgtcta caccttcaag
    20101 ctgcgcaaga caaagtggtc aaatgggcag cctgtaaccg cccacgactt tgaatgggcg
    20161 tggaagcgcg cactggcgcc cgagacagct tccgagtatg catatcagct gtggtacatc
    20221 aagaacggaa gaccgtataa cgagggaaag ctgaaagacc cgtctaaggt tggcgtaagg
    20281 gcagtcaatg actatactct ccaggttgtg ttggagaatc cgacccctta cttccttcag
    20341 ctctgcaact tcccaacttt gatgccggtt taccgcaagg ttgtggaggc ggatcctgaa
    20401 gggtggttcc agaatcccaa gaactacatt ggaaacggcc cttacaagat ggtatcgtgg
    20461 cagcacaact ccaagatcga gctggtgccc aacccgcact attggaatag gaagaaggtt
    20521 ggcatggaaa ggctcgacct ctatctggtc gatgatattc agaccgctct ttcgatgttt
    20581 gagaccggtc agctggattg gttcgatgcg cctcctgctg cagaaataga acgcctcgag
    20641 aagcaagggt tactacataa gtcggagtac atcggaacct actactacat gttcaacgtc
    20701 actaagaagc ctctcaacga cgtgagagtc cgcaaggctc tcaccatggc tatcgaccgt
    20761 cagactctga tagaccgtgt cgtcaggggc ggacagcttc cggcactggc ctatgtgccg
    20821 tacgggctcg gcgatgcaac gcctgataag gacttccgga aggttggcgg ggattacttc
    20881 aaggaagatc tcgctgctgc aaagaagctt cttgctgaag caggatatcc ggatggcaaa
    20941 ggcttcccga cgttgacgat tctatataac acgagcgaga accataagaa gatcgccgag
    21001 gcgatccagg agatgtggaa gaagaacctc gggatcgacg ttaggctgac caaccaggaa
    21061 tggcaggtct acctcgattc ccgcacccag ctcaactacg aggtggcccg tgcaggctgg
    21121 attggtgact atcttgatcc catgaccttc ctcgacatgt gggtgaccgg cggcgggaac
    21181 aacgatacgg gatggggcag caagaagtac gatgagctta tctatgcagc tcaggcagag
    21241 ctgaatacag ctaagcgtgc gaagatgctc catgacgcag agaccattct catgactgag
    21301 atgccgataa tgccgatcta cttctatgtc aacctgaccg tgatcaagcc gtggatcaag
    21361 ggcgctttca gatcgcctct gggcttcatc gacttctcga cagcatacgt gcttgagcat
    21421 tgataaggcg cttataggcg cgcacgtatc tatatgaacc atcagtgccc gggggcatgc
    21481 acgggttacc ggagcatgcc cccggctctg gcatttgacg ggagctgata ttggtgggct
    21541 catatgtcct acgaaggttc atttctgtaa tactggttat attcgtggtg gcgactgcga
    21601 cgttcttctt gatgcacgct atcccgggcg gcccgttcaa gagagagaag gccttgcctc
    21661 ctgcgattca aaggaacatt gaggaacggt ataaactgaa tgacccgcta tggaagcagt
    21721 atgtcgacta catcggcaac ttgctccggg gcgatctggg gccttcctac aagtatttgg
    21781 gccgatctgt taatgacatc attcgggagg ggttccctat cagcgcccat ctgggacttg
    21841 taagcgtcgt tttcgccctt ttggtggggg tcccgtgcgg gataatatcc gccctcaaac
    21901 agaatcagtg gcaggataac ttcataatgg cgtttgccat cataggagtg tctgttccaa
    21961 gcttcgtcat ggcaacattg ctcatgtatg tgtttgctgt aaagcttcag tggctgcccg
    22021 ctgctatgtg gggtactccg aaacacgtta tccttcccat gctggcgctg gcggggttcc
    22081 ccactgcttt ctttgcaagg ttgatgcgat ctagtacgct ggatgtgcta tcgcaagact
    22141 acatcagaac agccagatcc aagggacttt cctggggtgc tgtcgtctac aagcacgtga
    22201 tcaagaacgc tatacttccg gtggttacct atttgggacc tctgattgcg ggaataatca
    22261 ctggaagctt tgttgtagag aatatctttg ccatacccgg gcttggacgc tattatgtga
    22321 cgagcatcta caacagggat tacactacca taatgggtgt tacgatattc tacagcgcgc
    22381 ttttggtcat gctgaacttc ttggtagaca ttgcctactg ctggatcgat cccaggatca
    22441 agctgacgga cgcgaaggag tgagcagctg tggaatatag cgctgaaatg ttcaaaccga
    22501 ttaacaagga tatcgatagc gcggaagcga ttgtgcgtcc gtcagttacc tactggcaag
    22561 atgcgtggcg caggttgaag aagaaccgta tggccatggt ggggctatgg accatcgtgg
    22621 cgattctcgt gattgccata ttcggcccca tggtatcgcc gtacagctac tctgaccaga
    22681 atctagagga gcagaaccag tattgcagtt caaagcattg gctgggcact gatgatctgg
    22741 ggcgcgacct gttgacgcgc atcatgtacg gagccaggat atcgcttacg gtcgggtttt
    22801 tggcgagtct gctcaacctc actgtaggag tgatctatgg cgggatttcg ggtttttacg
    22861 gcgggaagat cgacaatatc atgatgcgca tagtcgaggt gctcaatgcc attcctatgc
    22921 tgctttacgt catattgctc atggtcattc tcgagcctgg tctgcagaac atcctgatag
    22981 cgctgggcct tgtatactgg ctcggtatgg cgaggatcgt gagaggtcag gttcttagtc
    23041 tgaaggagca ggactacgtt ctcgcagcgc ggtcgatcgg ggcaagcaat tggcagataa
    23101 tcaccaaaca cttgattcca aatgcgatgg gcccgatcat agtcaccgcc acgctcaata
    23161 taccgtcggc tatattcacc gaagccttct tgagcttcat tggacttggc gtgaatgcgc
    23221 caatggccag ctggggcgtg ttggcatccg acgcgctaca gagcttcagg tcatatccct
    23281 ggcagctctt cttcccagct atagctataa gcattactat gttcgcgttc aacttcctcg
    23341 gagacggtct tcgagatgcg ctcgatccca agcagcgcaa gtaaccggtg gacatagagt
    23401 aggggggata cctcttggac agacttttgg aagtcagaaa cgttagcaca tcgttcttca
    23461 catattcagg cgaagtcaag gcagtccgtg atgtgagctt ccacgtggac accggtgaag
    23521 caatagctat tgttggcgag tctgggtgtg gtaagagcgt cacggcgctt tccataatgc
    23581 aactggtggc gccgcctggg aagatcacag acggggagat cttcttcaat ggacgcgatt
    23641 tgttgaagct cagcgagaat cagatgcaac atgtgcgcgg aaacgagatc gggatgatat
    23701 tccaggatcc tatgacgtct ctaaaccctg ttttgacgat tcagactcag atagtggaaa
    23761 gcatcatgct ccaccagcat gtcacccggg agcaggcggt caagagatgc gtggaactcc
    23821 ttcacatggt agggattcct tcgcccgaac gccgtttggg atcgtatccc cacgagttca
    23881 gcggaggcat gaggcagagg gtgatgatag cgatggctct ggcctgcaac ccgatgcttc
    23941 ttattgcaga tgaacccaca acggctctag atgtcacgat tcaggctcag attataagcc
    24001 tcatggcgga gctgaagaag aagctcaata tgtcaatcat actgatcacc cacgatctcg
    24061 gtgttgttgc gggtttggcc cagcgcgtgt tggtgatgta cgctggaaaa gtgatagaga
    24121 ccggtacagt acgcgacatc tattaccatg cccagcaccc ttacacatgg ggccttctga
    24181 agtccgtacc gaggctggac gcggaagaga agaaaaaact tgtgcccatt ctgggacagc
    24241 ctccggactt gataagtcct ccgaccgggt gtccgttcgc tccgaggtgc gattacgcga
    24301 tgtcgatctg cgccaatgag ccgcctgact atactaagct ctcggatcat cataaggtgg
    24361 catgttggct gcagtacccg ttggcgccga catatgatcg cgtggagaga gggggcgtgg
    24421 cctgatggag ccgttggtcg aggtgaaaaa cctgaagaag tacttcggtg ttggcggagg
    24481 aaccttcaag agcaaggaag ggacgctgaa agctgtagat gatatcagct ttagcatacc
    24541 ccgaggcgag actttggggc ttgtgggcga gagcggttgc ggtaagacga ctgcgggcag
    24601 gactatatgc aggctttacg agcctactgc tggcgaagtc atattcgacg ggattcccat
    24661 cttctcgctc ggtcccaagg aaatcctgcc tataaggcga aagatgcaga tgatcttcca
    24721 ggatccttac gcttctttga atccgcgcat gactgtgggt gacatcatcg gggagcccat
    24781 tgacatacac aaccttgcga gcagcaagga ggagaggctc gacatgatac atgaactcct
    24841 ccacactgta ggcctcagcc ctgagcatgc taccaggttt ccgcacgagt tcagcggggg
    24901 ccagaggcag aggatcgggg ttgcgcgggc tctggctgtg gagccgagtt ttatcatatg
    24961 cgacgagccc atatccgcgc tcgacgtgtc cattcaggcg caggttataa acatgctcga
    25021 ggagttgcag gaagaacgcg atctgacgta tcttttcatc gctcacgacc tggccatggt
    25081 taagcatacg agccaccgtg tggcagtgat gtacctcggg aagatcgtgg aacttgcaga
    25141 cagtgtcgag ctttacaaga acccgcttca tccttatact aaggcattga tgtcggcgat
    25201 tcccattccc gacccggatg tggaggctaa gcgcgaaagg atcattcttg aaggggatgt
    25261 cccgagcccg atcgatcctc ctccaggatg caggttcaga tcgagatgta gctatgccat
    25321 gaaagtctgc ggggaagaag aacccgagct gcgcgatttc ggcgggcatc atatcgcctg
    25381 ccatcttgta aagtagactc ccatcgtagg gacgcagacc cttgacgcac ttgttcgtgc
    25441 ggggggtgct gagcagtaca ggtttttggg gcgcatgggc tcagcggcac aaactttaac
    25501 gccgctgagc gatgcgcccg ttctcataac ctcatcgcct gcgggcagaa tctgtagtat
    25561 gagatatgaa gatcgtttgc ttcgaggagg ctctaaattg ccaaagagaa taagttctat
    25621 ctatgctcgc gccatatccc tgtcgactgt catcattctt gcaatggcag ctttcgcctg
    25681 ttctgcacag acgggggttc ctgcccaagc caaagtcgaa ttctacagcg aagctcctgc
    25741 gccgatgatt gaggcgaatg ccgccattct tgaggatgtc gcgaccggcc aggtcctgtt
    25801 ctccatgaat cctgatgaaa ggctggcgcc tgcttcgata acgaaagtca tgacgatgtt
    25861 gatcgtcatg gagcatgccc aggaaggcaa ggtttcactg gatgacactg ttgtcgtgag
    25921 ccgtgaggca tctctcatgg ggggctccca ggtttacttg aaggagaagg aagaagtccc
    25981 tatcagggag ctgttggcgg ctacagccat aaggtcggct aatgatgcaa gttacgcgct
    26041 cgctgaagcc attgcgggga gcgcagacgc tttcgtcgaa ctgatgaatg cccgggccgc
    26101 tgaacttggc atgtcaaaca cccattttgc caacccggaa ggcctcgacg accctggcca
    26161 ctacactacg gccagagata ttgcgataat gtcccgcgag cttctcaagc accccgagat
    26221 tctggagttg accagcactt ggatagggaa gatgcgcggg gggacctacg atctcttcaa
    26281 tactaatagg cttatcaagg tatacgaggg cgcggatggc ctgaagaccg ggcagacgga
    26341 caccgccggc tggtgtttgg tggggaccgc tgtgcgcggg gagacgagac ttctttcggt
    26401 agttcttggc gctgcgagcg atgagaagag ggtcggagaa acctcgaagc tcttggatta
    26461 cggtttccag aagtttgagc gaacgcgctt cttagatgct ggcgcgcctg tagggacgat
    26521 tcgtgttccc tctgccgcaa ggcaggatgt gacggcagtg gcggcagagg accttgtgtt
    26581 gctttgccca aggggatttt catctgagac agaggttcat tttgaacctc ttgggcagga
    26641 gttatctgct ccgataaaga aaggacaacc tctgggtcgc gcagttgcgg taatgccgtc
    26701 tggagaggaa ttagggtcga cgctcgctgt ggctgagtct gacgtcccac gggccaatgc
    26761 ggtagtgagg gcgtggaggg cggtagttcg cttctttgcg gaactgtttg gcggtagaac
    26821 tcgagcatag tagtttgtgc attacctgat ggatgtggtc tcatgcgtgt ggttgaaagc
    26881 aaagcttggg caaaaatcaa tcttgctttc gatgttgtcg ggctcagacc ggacggttac
    26941 catgatatcg aaacggtgat gcaccgtgta gagcttgcag acgagattag ggttgagcgg
    27001 cttgaagctg gtgcagtgtc ggaatatgag ataagagttg atgtcttccc gcaggtggcg
    27061 ccggctggta cggagaatat tgcgtatcgc gcggctatgg ctttttttga agccgccgga
    27121 gaacgaggcg cggtatcgat agggatcgga aagaagatac ctgttgccgc cgggctcggg
    27181 ggaggaagcg cagacgcagc cgcggttttg gctagcatga atcggctgtg ggatgtgcca
    27241 ctcgcagagg atgaactgta ccatgttgcc cgttcggtcg gcgcggatgt gccgttctgt
    27301 cttatgtgcc atggcgatcc gcctcaggct tgggcggcta tggcatgtgg cattggggat
    27361 gatctgacgc ccttgcctcc gctgccagga gttgccgtgg tccttgcgaa accctcagtc
    27421 tctctcctgg cgggcgaaat gtacgggatg tgggatgctc gacgagcgcg ggcgatgggt
    27481 gggcgcggca atgcgagggc tcgggctctg gcgaactgga tgcgatcgca aggcgggagc
    27541 gctttgaaag gggatgcggt atctggttcg cctgtggcgc aggtggtgca tctgattggg
    27601 aatgagctgg agccggttgc tgccgagaga tgcccggagg tgctcgaagt taaggcggcg
    27661 ctgctggagt tgggggctat ggctgcggtt atgagcggca gtggacctac tgtattcggg
    27721 ttgtttgagt cggatgattc cgcacattct gccgcacgga gcctcaggaa acgggcgttc
    27781 tctctttccg gaaggggccc gctcgatgtc atcgttacac atgccgggcc taggattcct
    27841 cctgggtctt aggagcatat cgaaggattt gcgaggcaca caattcaatc ccaaataggg
    27901 aatgaggtga gaggcgcaat gcgagtggat gtggcaattc tggccggcgc ggataacgac
    27961 gggcgtctgg ccgaggtttc agacgcgaaa tacgaggccg tagtggacat aaatggcaag
    28021 cccatggtgg aatgggttct cgatggcgtg ctgggggcaa aatctgtaga ccgagtggta
    28081 gtagttggcc cgtcagatgc tttgcagcct attgttgaca ggaaaaatgc cggcgtcagg
    28141 gttcaggtgg ttgaacgccg ggggaacatg atcgcaaatc tcatggctgg gctcgacgag
    28201 atctctgatg agaaaaaggc cgtggtgatt gcgggtgatg ctgcgtttat caccagcgag
    28261 tctattgacg gattcgtgga gatgtgtgag cgcgatcccg ccgaggtcca ctacgcggtg
    28321 gtggcgcgcg acgtgattga ggagaagttt cccggcgtaa aaagaaccta cataaagctg
    28381 gcggacgcga cggtgaccgg cgggaacgtg ttcatggtgg acgcgcctgt ggtccgaaag
    28441 aaccgcggca ttatcgaaaa ggctcttgac atgagaaaga accccctcgc attgggttcg
    28501 cttctggggc tgccgataat actcaaatac ttgtttggac gactctcaat cgcagatgtc
    28561 gaaaagaggg catgggataa gctggcactg gttggtcgag gcgtcatagt cccctacgcg
    28621 gagataggcg tagatgttga caagcccagc gatctggaac ttgctttgca gatgcttgcc
    28681 tgagcgagtc tccataatct actgtgcaat ctgtcgcgcg ctggttgatt tttcgggagg
    28741 tgtaaatgtt gaagaaggtt gtggatactg acagggcgcc agcagctata ggcccttatt
    28801 cccagggtat tgtagctgga cagtttctct ttgtttccgg ccagactccg ctcgacccgg
    28861 caaccgagaa gctcgtggat ggcgggataa aggagcagac taagagggcg ttggacaaca
    28921 tagttgggat cttggaggct gcaggaactg ggctggataa ggtggtcaag gtgacggtct
    28981 atcttgaaga tatgaatgat ttcgctgcca tgaatgaggt ttacgcctcc tatttcggat
    29041 ctgaacagcc tgcacgatgc gcaattgagg tctcgaggct tcccaaagac gcaaaggtgg
    29101 agatagacgc catagccatt ttgtagcaaa tgcattaaaa acacgaaaaa tgcgccctct
    29161 ttagaggatt ttcctggaat gcgtagaaca aggatacaaa aggggaacca ttactgcatt
    29221 tccatgaaag gtgggcgcgg tgtgaacatc actgacgtga gggtgcgcaa agtcaacact
    29281 gcaggaaaaa tgcgggctat tgcgtcgatt acttttgatg atgcattcgt ggttagggac
    29341 atccgggtta ttgagggaca gaaagggtta tttgttgcca tgcctagccg caaggctcct
    29401 gatgggcagt tccgcgacat tgctcaccct gtcacgcagg aagcgcgtga catgatcgaa
    29461 gacgcaataa tatctgtcta taacgaacaa ggcgactagc gcctcttgtc taagcggctg
    29521 agtttttggt attttgcctc aggcgctttg cagggtttgc tattgagacc gaatggctgg
    29581 aagaattcta ttgacggtat tgtttcaaca tctgtttcgc ctgggttctc gttaggtgtg
    29641 gccgctggcc gcccgggatg ggggctttga ggcggcgcag tgttatatag caactgcaga
    29701 tcggtcaaag agcatctgac ggagtgtgca tgccttgcgt attgcgaagg ccaattctgg
    29761 gttccccgat gcgccacgcc tggatgcttg aggttccgtg ccttggtggc tcctgagatg
    29821 agccaccttt tatatcgtgt tttcgttgag atggcgtatt tctcttgtca ggcgttcgag
    29881 gcgtgtaagg atggaaactc tccctctcgt caaatatttg ctcaccggct tttgttatgc
    29941 taaaatatgt actgctttag gatggggcgt agccaagagg taaggcacgg gactttggat
    30001 cctgcagccc aggttcgaat cctggcgccc cagccattat atataggcaa gctgtatatg
    30061 cgacggataa ctttctgaca gggcgattgc cctgtttttt nnnnnnnnnn nnnnnnnnnn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnn nnnnnnnnnn cggcgaaaag gatataagtt cgaatattct tacacggcga
    30241 tggggatagt atactgaaag ggcgactcat gcaagaaagt ggccgtacac cggatataga
    30301 ctatgggcgg gcgaggctga ggattgcccc gcccgcaagt gaacgtcgcc cgtcgccttt
    30361 gaagaatatt caccatcgcg gtgcatgtgg tttcagactg atggggctcg gggacggcta
    30421 gaacgaggtt ccgggtaagt tgcctgaagt cggaaaggtt gacggacctg acaagttgcc
    30481 ttcctgtgcc gtcaagttct gtgcaggaat gacttcctga aagccctgct taactccagc
    30541 catggttgtt gctgcaagtg aggcttgtgc cgcctcgatc atccgccgaa ccatgtttcc
    30601 gccgacagat ccacaatcgc gggcgctgag atttccccag taatcccctt caatgagatg
    30661 tgttggaatg ttgagttcgt tggcaacctc atatttcatc tgatctaatg cttgccgcgc
    30721 ttgggggact agtgttctat tgcttctgcg agcctttggc atgttttcac ctccattgcg
    30781 atgctgagta cattctttgc cgagctaggc ggggatatgt tagtaaacgg atgtcgatcg
    30841 gcactctgtt tgccagcatg gtgaaaagtt gacgaagcag tatgcttggt atatgctcaa
    30901 agtgcttgaa gaccagtttc aacattggat taatttggcg gagtgaataa tatggcatct
    30961 ttcgtgggcc ttgtactagc ggcagggcat ggcaagcgga tgaagtcagc gcttcccaaa
    31021 ccacttcatc cagttgccgg catacccatg attgaccacg tcgtgggctc gttggttcgc
    31081 tccggagcat cgcgtgtagt agttgttgtt ggacatggtg gcgaattgat tgaagagcat
    31141 cttgctgggc gcgcagaaat agtctggcag cgcgagcaat tgggcacggg ccatgctgtc
    31201 ttagcggcgg gcccggcttt tgatggttat gatggcaata ttctcatcgt acctgcagat
    31261 gtgcctctgc ttacttccga aagcctccaa caactagtag aagagcagtc tggcagcgca
    31321 agcgacgcca caatcctttc gatgtgcgtt gatgacccta cagattacgg gagggtggtc
    31381 agggactcga aggggaacgt cgccgccata gtagagcact gcgatgcgac ggaggagata
    31441 cgcgctattc gcgaggtcaa taccggcgtt ttttgttttg acagtaggac acttttcaac
    31501 gcgcttccgc gtatatcaac agataacaaa cagggcgagt attacttgac agacgtaatt
    31561 gcgattatga tctcccaagg gctgtccgtg agagcggttg cccttaagga tgcatcagag
    31621 ggactgggaa tcaattcccg cgtcgagctt tccaaagccg agcagatcat gggcgatcgc
    31681 aagcgacgcg aggtgatgga atcaggggtt actctgatcg atccggcaac cacgttcatc
    31741 gatgcaagtg tggagatagg gtccgacacg gtcattatgc catttacaat catagccggg
    31801 aacagcaaga ttgggggatc gtgcaatatc ggcccgtatg tttacattcg cgattcagaa
    31861 gtgggcagcg gatgcaatat tggcccattt tcctatctgc gcccgggcac atcacttgcc
    31921 gaccgcgtca aggtgggcga ctttgttgag gtgaagaaga gcacagtagg cgagggaagc
    31981 aagatccctc accttagtta tataggagac acggtcatgg gctcccacgt aaacataggg
    32041 gctgggacaa ttacatgcaa ctacgacgga gaaaccaaac acactacgat catagaggac
    32101 ggcgcttttg tgggggcgaa ctctaatctg gttgctccgg taaaggttgg ctcggacgca
    32161 tacattgcga ctgggtccac agttacttcg catgttccag cgggggctct ggccatagcg
    32221 cgagcgaggc aggagaacaa ggacggatgg gtcgcaaagc gaagggcggc tcgattatcc
    32281 aaggaggaga agtagctatg gactcatgtc atcgaaagtt gaagatcttc gcgtgcaatt
    32341 ccaaccctga gctatcgaag gcgatagctg ccaatctcga aacagacatt gtcaaagctg
    32401 aagtgggcag gtttagtgac ggtgaaatca gtgtacaaat attcgagagc ataagaggcg
    32461 cagacgtctt tgtcatacag tctttgtgtg ctcccgttca cgacaacatc atggaacttc
    32521 tcatcgtcat cgatgcgcta aagcgcgcat ctgccaggtc tatcactgct gttgtcccgt
    32581 attacggcta tgccaggcag gacaggaaat ctcagcccag agagcccatt acagcgaaac
    32641 tactggccaa cttgatcact gctgcagggg cttcccgggt tctcactatc gacttgcatg
    32701 cgccccaaat ccagggattc ttcgatatac ctgtcgacca tctcaaggca gcccctatcc
    32761 tcgcgcagca tctggaatcg aggggcttga agggttgcgt gatcgtatct cccgatgtgg
    32821 gcggggtaac tcgcgccagg ggaattgctg agcaccttga cagcacgttg gccattgtgg
    32881 acaagcgtcg cccgcggcct aacgtgtcgg aggttatgaa cataatcggc gatgttcgcg
    32941 gcaagccggc ggtgatcgtc gatgacctcg ttgatactgc gggcaccctt tgtcaggcgg
    33001 cggacgcact tatcgaggcg ggggcgactg aagtaagggc atgttgcacc catggagtgc
    33061 tttcggggcc ggcaattgag cggcttgcca attcgcagat ttcggaactg gttattaccg
    33121 atactattcc cttgagcaaa gagaaacaga tcgacaagat atcgattcta tcggtggccc
    33181 cgctttttgc ggaggcgatc aggagaatct atcttgagct gtcggtaagc atactatttg
    33241 attagtcttt tacagcgggt agtgacatgt tcattatagc aggccttgga aaccctggaa
    33301 cccaatatga gtgctccagg cataacattg gcttcatggt tgccgaccgt ctcgcattgg
    33361 agctcggcgc cggcaagttc cggcgggctc cgcattgtct cagtcttgtt gcaaagggaa
    33421 cgattgcagg gacggatacg ctaatcgtga agccgcagac ctacatgaat aacagcggaa
    33481 tcgccgtagc aagagcgacc cggcagtacg gcgcaggccc ggagtcgctt attgttgtgt
    33541 acgatgacat ggatctcgag gtggggcgca tacgaattcg ggcaaagggg agtgctggcg
    33601 gccacaaggg tatgggctct attatagaac atatgggcac tgagaatatt gcccgtgtgc
    33661 gcgtgggtat aggaaggcct cccccatggg aggattcggt ccatcacgtg cttggcacat
    33721 ttgcccgcga tgaacttcct atcatatcgg atgctgttat aaacgccgtt tctgccatac
    33781 tatgtattgt agaggacggc atcgagaccg cgcagacccg cttcaattga gctgggtctt
    33841 gcggtgtttg taatgcgtca tctttcccgg ggggtatggg gttgacagga atagtcgacc
    33901 tcctgctcga ctctaccgct ttcacggaag ctttgagaag agttgagcgg ggatcgcgcg
    33961 ctacacatat tgtcggcctt tcgggttcgc agaagtccct attcatatcg gggctgtttt
    34021 gtcatgcccg gaggacggtt ttcgtcgtta ccgcgacaca gcatgacgcg gaaagcttgt
    34081 cgggggatat tgcgactatg atcggttccg atcgggtctt tgtcttccca gcggtagatg
    34141 tgatgcctta tgaggagacg ccgatgagcg ctgacctttc tgcggctcgt gccaaggcca
    34201 tggctgctct ggccagaggc gaaaagtgcg tggtcttggc gccggcgcgt gctctcggaa
    34261 gaaggatggt tcccgtggag attttccggg gcgccatgtt cgatatcagg gtcggccaac
    34321 gctgcgacct cgatggcctg gtatacatgt tggctgcgag cggctacgag attttgggca
    34381 aggttgacgg ccgtgggcaa gccagtgtgc gagggggcat agtcgatgtc tttcccaatg
    34441 atgcggaccg gcctgtgcgg atagagttct ttggcgacga gatagagtcc ataagggtat
    34501 ttgaccctga cactcaactc tcacaggaga tgttggatga ggcgactatc actcccgcgc
    34561 gggagttcat gtatgaccgg gaacgtctgg aacgtgcgcg catgtccatc cgggaagagc
    34621 ttgatcgggt gggcgctggt gctgcgggca agggccggtc ggtctcggca gacagtgcgc
    34681 aggcgccgtc gatgctgtct ccttcaagca tggtgatggc tcatacaaga aatcagctgc
    34741 gtgaaagggt tatgcatcat ctcgagcgct tcgaaacagg ggcctacttc gacaatgacg
    34801 agcagtatct cccgtttttc tactcggaac gggtgatggt ggcggactgg gccgaggacg
    34861 ccctgtttgt cttggatgaa ccggtccggc taaaagaagc tttagatggc tacgagtcgg
    34921 acatacgcga gacatatgct acgctcctcg aagccgacat ggttcttcca tcgcaggcag
    34981 actcgtatat gaatgccgct gaaatcatcg atgccatcaa gcgtcgacag atagtgtcca
    35041 tgtctttgct cgctagggcg ccggagggca gctgtggagc cggccctggc ggcgctttgg
    35101 caggttacgg ggatcatata attgcccaga cgaaacaggc ggagatattt gcaggccgca
    35161 tcgacgattt tattcacgag atcaagaact tccggaaacg aggatacagg gtagtctgta
    35221 tggcctcgac ggaagagaga tgcaacaggc tggctgaact tctcgaaggc aaaggaatct
    35281 cgccgatttg tgcttcgacc cttgatgttg tcccggaggc gggcacggca agtgtagtcc
    35341 ggggcgagtt gtcttcgggg tttaagtgtc ccgatatcaa gcttttggtt cttacagaag
    35401 ccgagatata tggccggcag aagaagaggc ggcggttcgc cgatgtcgag gaaggcgcgc
    35461 ggatcgccag ttacacggat ctgtcgcccg gggactacgt agtccatgtc aaccacggag
    35521 tggggaagta tggtggggtt cagacgttgg aggcggcagg ggtccaacgc gattatctgc
    35581 tggtccagta tgccggggaa gacaaggtgt atgtccccac tgatcagatt gcccttctgc
    35641 agaaatacat aggaatggaa gattcgcccc cccgcctgaa tagacttgga gggacggaat
    35701 ggacgcgcac caaggcgaag gcgcgggagt cagtccagga catggccgag gaccttcttc
    35761 gactttacgc agccagggaa tccgtgaagg gctacgcatt ttcgccagac accgtatggc
    35821 agcaggagtt tgaggatctt ttcccttacg aggagactcc tgaccagtgg cgcgcaatag
    35881 ttgatgtgaa aagggacatg gaaaagccca tgccaatgga cagactgctc tgcggggatg
    35941 tgggatttgg caagaccgag gtggcgctga gggctgcgtt caaggccgcg gtagacggca
    36001 aacaggtcgc ggtactcgtg cctacaacga tccttgcgca gcagcactat aatacgtttt
    36061 ccgagagatt ccacgggttc cccatgaagg tggcaatcct atcgaggttt caatcggaag
    36121 cggaacaaaa acaggttatt cgaggtctaa agatgggatc tgttgatgtt gttatcggaa
    36181 cccatcgcct gctttcgcag gacgtgaagt tccgcgatct cggcctcgtg gtggtcgacg
    36241 aggaacagag gtttggcgtg gctcagaagg aacgtctgaa ggagctccgc aaggaagtgg
    36301 atgttctcac gctcacggca acgcccattc ccaggactct ccatatggcg atgacagggg
    36361 ttcgagacat gagcctgatg gagactccgc ccgaaaacag gttccccata aggacttacg
    36421 tagtggagaa aaatgacagc cttgtgaggg aggtcatttt gagggagatc gaccgaaacg
    36481 ggcaggtcta tttcgtccac aatagggtgc acgacataga agaagtggcc ggaaggctga
    36541 ggcggcttgt acccgaagca tccatcggcg ttgcccacgg gcaaatgccg gaggagaggc
    36601 ttgaaagggt aatgttacaa tttatggaac acgagtttga tattcttgtt tgtacggtta
    36661 taatagaatc tggcattgat attcccaacg tcaacacgat aatcatcgat gatgcggata
    36721 acttcgggct tgcccagctc taccagttgc gtggaagggt cgggaggacg aacagggtgg
    36781 cctatgccta ccttctgttc aaccgcgatg cggctctgag cgagatcgca gagaaaaggc
    36841 ttgctgcgat tcgcgaattt acgagcttgg ggtcgggcta caagatagcc atgcgggatt
    36901 tggagatacg gggagcaggt aatattctcg ggccggaaca gcacgggcat attgcggcgg
    36961 tcggatttga gatgtattgc aggctcttgg aggaaacaat aaaggaattg aggggcgaga
    37021 agaaggagcc cgaggttcct cccaccgtga tcgatcttcc tgttgacgcg tatgttccag
    37081 atagctatgt ccccgattcg cgtcagaaga tagaagtgta caagaagatc aacggcatcg
    37141 aatcgttaga agatgcttct gacatttcga aggaattcat cgatagattt ggaccattgc
    37201 cgcctcccgt ggaaaacctc attgccattg ctgcgttgaa aggtttggcg agggatctgg
    37261 gggctgtgaa tgtttccagt gaaaggcaga gtgtggttgt ggggtttgct gatggggtga
    37321 gctactcagc aagcgacctg atgaggatcg caaggccgtt taggggaaga gtggcacttg
    37381 cggtggggag aacgcaaaga ctcagggtca agacgcacgg gatgtcggaa cgagaaatct
    37441 tgcatacgct gataggcatt ctgtccgaga tgaagcaggg cgtagcagtg aatcaatgac
    37501 caaattacgt agtgatatgc aggagggtat tgtggatgag acctactgga attgtgaggc
    37561 gcatcgatga gttgggcagg attgtggtgc ccaaggagct cagaaggagc cttcgaatac
    37621 atgagggcga ttcggttgag atatatgtgg atcccaaggg aagcgtggtg ctgaagaagt
    37681 actcgcctgt ggggcagctc aaggtcgtag caactgacat ggccgaatcg ctttcggcgt
    37741 catcaggcag tgtagcgctt atatgcgata gggatgttgt gattgctgca tcgggtccgc
    37801 tcaaagagga gcttatcgat cgtcgtgtag gccgtgcggt ggagaaggcc atggccgaaa
    37861 agcaggcatt gatggtccat tttagcggag gcggggatgc gtccagcata ctggcgaatg
    37921 atgggccgga ttccgtagcc atgatttccg ctgcaatagc ccctatcatt gcagatggag
    37981 accctgtagg cgcagtagtg atcggaacta ccgctagtga ccgcactgtg ggcgatcttg
    38041 aggaaagtct ggctatcacg gctgcggggt atttggcaag gcaggtggag tagacgaaca
    38101 gcaaccggga tcagacgtca tttctccggg gggccgccat tatggcggct gccggagtga
    38161 tcagccggat actcggcgct gtctatcgaa tccctctcgc tcatactatc ggcgatgaag
    38221 gtatggggtt gctcgagctt gcgcaaccct tatataattt ttttcttgtc gcctctgtgg
    38281 cagggctgcc tcttgccgtg tcaaagcttg tagctgaacg cattgcagtc ggggatgagc
    38341 gcggatcgtt ccgcatactg agggttgcaa ttctcataat gctatgcact gggcttgcat
    38401 cttcgtcact gatgtatttc ggggctgact ggtttgcaga acgtgtctac catgatgtgc
    38461 gggtagctct tgccatgagg gcaattgctc ccgctctttt ctttgtcacg gtgatgtccg
    38521 cttttcgcgg gtatttccag gggtgtcaag acatggggcc gtccgctgca tctcaggttg
    38581 tggagcagct tgtgcgcgta ggtaccatgc tgactttggc tgcagcgttg atgccccgcg
    38641 gggtggagta cgccgctgcc ggcgccgcgc tgggagctgt tacaggcggg atggccggcc
    38701 tcgcttactt ggggatcgcc tttgcccgga ggatgagcgc cggaggggtt ggaagaggca
    38761 aatcggccag gagcaagaag gccgcgcgcg catgtatccg cacaggcgtg ggaaaccggg
    38821 cgacaaagga agagacaaca tgggagttag tggccgacat cttctcgatt gcgctgccgg
    38881 tggccatcgg cgcttcggtg ttaccgattg tcagagcgat cgattcggcg atggttccgg
    38941 cgcggcttgg ggctgcgggc tttacggccg aaacagctac ggccctattc ggacagttga
    39001 gcgggatggc actaccactg gcatacttcc ctaacgttct cacgattgca ctggcaacta
    39061 gcatcgtgcc ggcgatctcc gaatccctgg tggctgggag cagatcgagg gctttgaggc
    39121 gggcagggag cgcaattagg ctgacagcgc tcatatccct tcctgcagcg ttcgggctct
    39181 ggacactcag ctctgagata tgtagtttgg tctatggaag acctgaggtt gggcgggtgc
    39241 ttgcggcgct tgctccggct tcggcttttt tgtgcgtatt gcagacatca ggcgcgattt
    39301 tacagggcat gggttatccg ggggcgccag tcaaaaactt ggtggccggt tcggttgtaa
    39361 aggtggtatg tgaatactgg cttacaggtc gcccgcggct gaacgtggta ggagcagcga
    39421 taagcacctc tgtgggattc ggggtagttg ccctggcaaa cctggctgat gttgcaagaa
    39481 ggcttggtca tggcgcaggg gctcgccttg cggcggagtt tgtaaaaccc gcgatatccg
    39541 ctgcgataat ggcggtggta gccaggactg cttactcatt cctcgtccgc gccactgtac
    39601 gggcagggct tgccacaatc gcatcaatga tggtggcagc tgtagcctac tttttggtga
    39661 tgcttgtcat cggcgggatt gtcggtgccg acatcgaaat gatccctaag ataggaccgc
    39721 ccctcgcgcg cgtcctgggg cggatcggtt tggtcaagtc gtagtgacga attactgcgg
    39781 aaggtgtggt agacatggca gacatcgaag agatcgatag attgcagagg cttgaagcag
    39841 cccaaaggac ggtgaaatcc gatgcggctt caggggctga agtattaggt gagagtcaat
    39901 gcagataccc ggcggacaag ctcgtagaaa tcatgtccag gctccggggt gaggacggat
    39961 gtccttggga tcgcaagcag acgtcgtctt ccttaaaacc gtatgttata gaagaagcat
    40021 acgaagttgt ggaggcgatc gataggcaag atccgaatct tctctgcgaa gaactgggcg
    40081 acctgctcct gcaggtggtc tttcattgcc agatcgcccg cgaagagggg gccttcgatt
    40141 tcaatgatgt tgtggaggct atctgcgcca aactggtgcg tcgccaccca catgtgtttg
    40201 gtgatgtaaa agcggacgat gccggcactg tcttgcgtaa ctgggagcgg ataaagcaga
    40261 aggagaagga gtcgtccaac ggggcgcccg cttcgattct tgatgaggtt cctaggtatc
    40321 tgcccgctct catgcaggcg gtcaagatcc aggataaggc gagcagggta gggtttgatt
    40381 gggaatgtcc ggaggacgca gccgcaaagg tttgggagga gacggaagag ctccgcgagg
    40441 ccatggcaga tggggaaatg gcaaaggtcg aggaggaact gggggatctt ctgttcgctg
    40501 ttgttaacgt ggccaggctc atgaagatag atccggagga ggctctgagg cgtaccaact
    40561 cgaagttcgc gtcgaggttc aagttcatcg aacacagggc cgatgagaac ggagtggctc
    40621 tcgaagaaat gactctcgcg gagatggatg ctatctggga tgaagcgaag gagaagggcc
    40681 tttaggtttc gagttgatgc attatatggt gggaatgggc atagacatcc ataggacaaa
    40741 ttgatgtgct ccatgagaga tgcgcgctat cgatgcaaag atttgtccga aggagtggtg
    40801 ccgatggccg ccaaaggacc tgacctgtct gatgaagcaa ggcggaagcg tccgggcttg
    40861 caacacaggg ttgtgctggt tgatcgtgaa agcatgacgg tggatggggt agctaatgtg
    40921 gagagcttcg atgacgagga gataattctg gagacgaatg cgggagcgct cactgtgacc
    40981 gggcatgatc tccacataag gcagctcaat ctcgaggacg gaacgatcga gatcgagggg
    41041 tatgttgaga catgcgacta tttcgacgag agcccgaaga aggggagagg cttgctgggc
    41101 aaattgctcc gataggctgg gctgatctac cagattaatt ggccctttgg gaacgtggtt
    41161 ggaggggcat cgggacgggg cgaagagtgt aatagacatt gaaaatagtt tatacacata
    41221 ggattcttgc gaaatgcgac gaactactgt gacagtgcag ggcgaaggaa tggaatagct
    41281 aggttgcatt gaatcggtct gagcaatcag aatatgcaat taacttggct ttaggagaat
    41341 acgccttgcc gggggaccac gtatgtcaag aaaacctaag cgcagcgcgc cgaaacggag
    41401 aaaaccgaca acacagaaga agagcagtat gggtttgggg ctcaggatcg ttaccgggat
    41461 cttgggcgtg atcatcagtc cggtcgtttt tgcggtatcc atattccgac cgtcgatgaa
    41521 gatgccgaag cgttggccga tatcgttgac agttctcatt atcattcttg ctgtctactc
    41581 ttgggcgggt gtgaagtacg tggatcagct ttccgtcgcc gccttagata tcctgggtga
    41641 ctacagttct gcaaggcttg aagtgaggga catgcctttt gcagagacga taaactttga
    41701 gggtttacgc aatgggatcg atccgtgcct tgtggcagcc gtgatatccc aggaaagcgg
    41761 gtttcagacc gatgcagtat cttggagggg tgcccgcgga ctgatgcagc tgatgccgga
    41821 cacatggcag gagttcatgc ccagcgccca gtgtagcggt gatcacgcgc cacctgcgtg
    41881 cgggaatatg tgcatataca atcctgacgc aaacatccat gttgggacgg cgtaccttcg
    41941 ttatctcata gaccgttacg atggtgacat tatagcggcc ctcgcctcat acaatgcagg
    42001 gatgtcggcg gtcgacgatg cgcaggcaga tctggactca atacaaaatg acattccgcc
    42061 ataccccgag actcaaggac atgtgggaaa cgtcgttcaa gcatggacga gtttccgaag
    42121 cgggctggca gaatggcggg tgcgcgcagt ggaacgggct gcacgggcac gcaatgttac
    42181 atcttggatc gcagtaggcc tcttgttcct cttcatgatc tgggccgccg tcagatatcc
    42241 tgaataagcg cgatcatccc cacggggcag aggtgacggc tgtggaaggc ctgaactggc
    42301 agatccgaac ggtgcttgca tcacttgctg cgggcatgct catgggagcg ctgtacgatc
    42361 tgtgcgcgtt ttttgcgcga gtgtttcgca tgcaaaaagg ggccatggca ttagtcgatc
    42421 ttttctactg gctgctctcc tccgctatga cgttttccat cttgatgcga gtaagccgag
    42481 gagaggtccg ggtatacgtt ctggcaggtg ttgctattgg cttttgggtg tatactctca
    42541 caggcaggtt gctcgccgcg cctgtattca ccataattga atggacggcc aggcgaatat
    42601 ttcggagcct gcggcgagcc tggaactggg ctcggcgacc ggtttcgagg acgaatcggc
    42661 aaatatccgg cgtgctcatg aagggatttt catcaacgcg gcgaaaactg tcccaagccg
    42721 ccaagtttcg gcgcggggca gaagacgctt cggaaaaacg cgggcgagcg gcacgcgaag
    42781 tcaacgagta aggaggcggt ggctgtgtcc tcaaaactca ccgttgtcct cacgatcgtg
    42841 ctcgtcgtgt gtgtgggatt gtgctatgcg ggcggacggg ccaggctctt ggcgctgagg
    42901 aaagagactg aatgtttgga agcagagtgc gaggaatggc agtccagaat acgggacctt
    42961 gaaagcgaga tcgaggaggc ccaaacagat gaatacgtcg aacggattgc cagggagaag
    43021 ttggggcttg tcaaaccggg cgagactttg tatatagtcg ctgaacctga cgccagcggg
    43081 tttactccgg tagcaaagcg caaagacaga ccggctgaga tcggggactg aggagaactt
    43141 tcacagtatg gtttgggctg taaatgttga gccactcatt gacactggtc aacgaattct
    43201 atataatatg aagtacataa tcatgagcat cagctcgtga tagttgaaaa aataggagga
    43261 tctctcttag catgtcgtca attgaagtcg gctctattgt tgaaggccgc gtcactggaa
    43321 tcaccggttt tggagcgttt gttgatattg gtggagggag gacgggccta gttcatattt
    43381 ccgagatagc cgacgaatac gtccatgacg taaatgacta cctgaagatg aacgacgcag
    43441 tcagagtcaa ggtaatctcg accgataacg gcaggattgg cctttcgatc aaacagaccg
    43501 atcctaacta ccagccgcga ggagggcgtc gtgatcgggc ataccagcag tccttcgagg
    43561 ataagctttc caagttcctc aaggaaagtg atgagcggca ccaagatctt aagaaacata
    43621 tggactccaa gcggggtggt cggggaacgc gcctgacaag gaccatgtag cccataaaca
    43681 taataaaaga tatggcactc ctctttgtgg agtgcttttc ttttcgtgtg atttagcctt
    43741 tcagcccgca ctgcacgatt ttccgcaagg tgccatgata cccttggatg actgcaaagg
    43801 acgcgtgtga ggggtgtgtt tgttgagcaa gaaactgggg aagtcgatag aaggcggcca
    43861 tgggagaact gccgaaatgc gtgaggagca tcaagacttc gtaaagccta gggccataga
    43921 tcgagcggtc gttgccgcgc agctcggccg agatctcatc ggcagcattg ctcgaattcc
    43981 ccggcggtgt tcttacgggc aaccacaggt tgtgtcgacc taccccgtca tatggcacaa
    44041 tggcgggctg gggcgggttg tgaaaccgaa gccctttcct acggtctact ggcttacatg
    44101 cccccacgta cgcgaagcca tatcggtgtt ggaaagtgag ggcatgatag gcgaggcggc
    44161 agagctgata gagcgggatg aagatttccg gaaggcgcac gagtgtgcca atacgaggta
    44221 tgctgcgcaa aggatggcgc tcataggcga agatgatctg gagtttctgg agaaagaggc
    44281 tccccaaatg cttcgggttc tcagagatac aggcataggc ggggtggcgc gctttgcagg
    44341 ggtgaaatgc ctccacatgc atgttgcgga ttacatggcg ggcaataaca atcctgttgg
    44401 ggacatggcc gtgagtcggc tgcgccaagc cggggtatgg cttgagtgtg acggggggag
    44461 atgtgttccg gcgcgggtgg cggcgataaa tgccggttcc aattcgacta aggtgcttgt
    44521 ggccgatgta gtgtcgcggc cgaattggtc ggcaggttct gacagctccg tctgctcaaa
    44581 gatcatgaaa gcatatggag attctagcgt tgttggggtt ccaagggttt tcggggtgtg
    44641 catggatgcc cggattaccg ggctcgggca tgggcttggg gaaactggaa ggttgtctga
    44701 ggcaggccgg gcagctactg tcgaagccat tagcgacttt atggggctca gtcgcagcct
    44761 gggagctgat agggtatggg tgacagctac ggcggcggcg agggcggcgg aggactccga
    44821 agccttgatc cgtcaagtta aagaggcatg cgaagtcagg ctcgaagttg tgtcaccgga
    44881 gtttgaggcc gaattgtcgt ttctgggcgt tgtggctggc gcaggttcag cagctgcggt
    44941 cggaagtgtt gcgccttccg tcgcgatcga cccgcgatcc ttgctgatag tggattccgg
    45001 gggcatgagc actgagttca cgaggctcga ttcttgcacg ggtgaggtaa gatctataag
    45061 ccttccgctc ggggcagtga gcctgacaga cgagttcctt tgttcagacc ctccatcgcg
    45121 gggggagatt gagcagatgc gtgggcgtat tcggttctgc ctggaggagg cgcgggagtt
    45181 tgtgcatggc ttaccgatgg acggggagga tggcggtatt ctatcgacga tagtcgtggt
    45241 aggcggatcc gctgttacac ttgcttcaat cggtttggag ttggaaacgc tagatccgga
    45301 catggtccat ggctacgcct tgcacaggga agagttggag gaggcatttc ttggcttgtg
    45361 ctcgttggcg tgtgctgaaa gaatgcaggt gaaagggatg atccagccgg agcgggccag
    45421 ggtgatgcct gccggggcgg cgataatcct tgaggtgatg gatcttgcgg gcgcggctga
    45481 ggttgtggtg agcgcagctg gaatactcga tggtatggcg gcctgcatcg ggttaggccg
    45541 ctgcggaagc aagttgtgaa atcggagctt ggcgctattt ctgtattcga tgggtctgca
    45601 ggaggcggct ttggtaatcc tttgcaggct acgtgcgtag cgcaaggggc gcggtcgtgg
    45661 tatacttttc ttgaggttat ctgcgacaat aaagtcgagg ccggagtggc ggaaaggcag
    45721 acgcagggga cttaaaatcc tctgtccgca aggacgtgcg ggttcgaatc ccgcctccgg
    45781 caccagctat ttgtagcggg gagtaggccc cgctgttttg gttttcagcc ccagcgggtc
    45841 ttattgtcga tgtgtttagg cgctttcgaa tagccatcgt gtgatggaaa ttcagagaca
    45901 gcgcagaagc agatcacaga ccgagcaaac tgcgttgcca gcggaaaatc agcttgccaa
    45961 tgaatctaag acggggtctt aaatacgtac acatgccgaa aaacaaccat aacgtcatct
    46021 ctcgggtcaa tatgatatcg gaacgtgacc ccgagtcgtg ggttttacgg cgcatccggt
    46081 agcggaaagc agttgtgtga caagctcatt ccgcattcag ttcatgggtt aatatctccc
    46141 ttatcttgcg ctcgagttcc tccaagtcga gtccggcctc ttccttaatg ctcttccgca
    46201 gcattgggcc gatctgttct atgtcgtcta gaagatcttg aaggagttca agggcggctg
    46261 tggcttcaag aacctctgtt ggggtcctga cgatgcttgc taggatggag ttctcggtcc
    46321 ttgaagcgat taatgggccg ggtagctggt caacgtagac cctggtacgc cgaccgggtc
    46381 cccgctcatc ttctatggga tcgagggcga tgatcttgtc tgaacgcaca tacttgccat
    46441 agccgagagg tatgatggca tgctccctga tttccatcgg agttcacgtc ctttagttct
    46501 gatctacttc aattaggttt tccagcaaag aacagtccca tcactccatc gtgctctgca
    46561 cagaaccatc ccgtatcggt tagcctacgc acattttgac tgttcgcctt cgatgggcaa
    46621 cttggtgacg aatagtatct gccggaccca ttcaagggtt cttactctat ccatgatgcc
    46681 ttctacggct tctaaccaca tcgcctatat ccttagacat ctcctcggta aatgaacctt
    46741 ggctgccgcg aacgccagcg gttatagcgg gcacttcatc cttgtgaacc tagtggcggc
    46801 tcaacatggt tatgcagctg ctgtaactgc agatcatggg ctgctcgaga ggtttgtgaa
    46861 tgaa
//
LOCUS       DAOSYL010000010        43343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_723_length_43343_cov_8.200818, whole genome shotgun sequence.
ACCESSION   DAOSYL010000010 DAOSYL010000000
VERSION     DAOSYL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 43343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43343
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_43343_cov_8.200818"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..831)
                     /gene="flgG"
                     /locus_tag="PLU15_03215"
     CDS             complement(43..831)
                     /gene="flgG"
                     /locus_tag="PLU15_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOO29962.1"
                     /translation="MMRALWSAATGMHAKQLDMDVIANNLANVNSAGYKKSRVDFQDL
                     MYQTIRSAGSTVAQGSRVPTGIQVGLGTRAAAIQKVFSQGDFVQTENPLDMVIEGDGF
                     FQVLMPDGSIAYTRTGAFKLDADGRIVTSDGYVMEPEIVVPENAVSISIGTDGTVSVE
                     IAGENEPQELGSIELARFLNPGGLTSMGRNLYRPTAASGEPIIGTPGLDGMGTVAQQF
                     LEMSNVKIVEEMVNMIIAQRAYEVNSRAIQASDDMLATATNLRR"
     gene            complement(982..1713)
                     /gene="flgF"
                     /locus_tag="PLU15_03220"
     CDS             complement(982..1713)
                     /gene="flgF"
                     /locus_tag="PLU15_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOO29963.1"
                     /translation="MLRGIYSAACGMRAQETRQSLIANNLANVATPGFKRDVGVGESF
                     SEMVVRSGRTPVGRLGMTSAITSAYFDMSEGSVSNTDCPYDFAIEGDGFFAVETDWGI
                     RYTRAGNFKLDADSYLVTMEGHRVLGDDGPIRVDGQLVAGSDGVLYADGREIGRLLVV
                     MPSSPDALTKEGGGLFAARGEVIPAGTGQVRVIQGALELSNVSVVREMVEMIAGYRVY
                     EAAQRALLSHDACLDRAINEVGRVG"
     gene            complement(1941..2402)
                     /locus_tag="PLU15_03225"
     CDS             complement(1941..2402)
                     /locus_tag="PLU15_03225"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOO29964.1"
                     /translation="MKAEFINPFASAVMMVFQKEFGMNVMREQLDLHSKGPLTGSDVN
                     TIIGVTGQLEGQVIYTFEEGVAIRIASALVGETVEELDEMAKSAVAELTNIMTGNAAI
                     SLSENGYSCVLTPPTLLTGKELIVTTFTPILTIPFMTDFGTVTVHVALREK"
     gene            complement(2457..3287)
                     /locus_tag="PLU15_03230"
     CDS             complement(2457..3287)
                     /locus_tag="PLU15_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29965.1"
                     /translation="MGCSDGDVVRALVPILGDRAWDVRVITDLSTKKSRAFLLCLPED
                     GPARECVMVAKIYRDEGRALREAEVLAALESAGVAVPAACGATPTVLLRRYVYGPVLR
                     DVHWTLELARKLGAWLYSCHEALRLAPQGVGGYGRTRLVGDMNLGNFVIDVAQADGDE
                     AVAWRDCGTRGIVCGIDFGDTRVGDPLEDVGEGFMRILSHRPGYTMERQACAAAFVWE
                     YGRLSGAEDMVWSEVERHVLAAFRRVAAWRRDPFMNDVAEAVGAMWGSGTPQIDWDGV
                     "
     gene            complement(3291..3971)
                     /gene="mobB"
                     /locus_tag="PLU15_03235"
     CDS             complement(3291..3971)
                     /gene="mobB"
                     /locus_tag="PLU15_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOO29966.1"
                     /translation="MRVIAVSGLHRTGKTTTVEHIVGELVRRGYAVGTIKTIHREGFS
                     VDTPGKNTHRHRRAGAKIAAALSPGETAVIIDGKLSFEDLFRYYDTDWIIIEGAHEAP
                     FPKIVCARTEHDIEERVDEHTFLICGEVASAISSWRGIPVLDATSRIGEVVDMVESRV
                     PEGSDRKMIDPAAYHGFKTAVYVDGKEIPMVPFVQDFVARTILGMLSSLRGYETGSEV
                     RVEIRRGE"
     gene            complement(4281..6140)
                     /locus_tag="PLU15_03240"
     CDS             complement(4281..6140)
                     /locus_tag="PLU15_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO29967.1"
                     /translation="MFKHTKAKLLVTFLLVVVFVLIGNVMMYLQLANYDRNMTQVVDE
                     ELLSLQLVESLRAYDLEAANAISGLIIDPGNAGFQRDYDVAWEKTENAMRAAARRLTR
                     PELVEALSEVDELGKSMSTVAVSVTGMAGLSPEMALSIFSDTYEPVRRQRAEILDGLV
                     RAERDHIGRQIASSKASVKRGTLVASLVSAIGIAAAVAMALRVSRAVSEPVERLTHAV
                     NKVAEGDLTVQSDVDSRDELGHLAQAFNKTVESLREIVGKALDVSRQVAAASVQLSAS
                     VEQSADSVRQIADTAQQISAGAEQQSRSAGETANAAEALMPTVERVAVCARGQTNSVG
                     RGSAAVDEMRSSIAQSLEGLKRVSSAAQGVGEAAREGSTATRDMAISVNLARRESARV
                     SEAARDLDSSSKEIRRIVEVINDIADQTNLLALNAAIEAARAGEHGRGFAVVADEIRK
                     LAERSLAETKAIAKLVEDLGRSTEKVAEAIGGSDSATAEVSESVDRTMEVLNRIILGV
                     QESVELIEALSETAGSLEGSSDRVRRAMAEIVAASDSSLKAAEDMNVGIGQVKESIDA
                     VAAVSFENVAAVQEVTASTEEVDAAIREMQASSQALADIAAGLEELISGFKLT"
     gene            complement(6235..8094)
                     /locus_tag="PLU15_03245"
     CDS             complement(6235..8094)
                     /locus_tag="PLU15_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOO29968.1"
                     /translation="MFSKIRGKLLASFLAVAVVMGIATGLLYFGLERYEVEVRRLATE
                     EIMGLKTAESLRTADLMLSNAVCGIISDPYEPIFAAAYEDALTKMETATREATSRFKS
                     GREKEIAAEIAAGNEERERVERKIIDCASFDRIQAAAIFTEEYNPVRVRREQVLDEFV
                     SLQERKIEEAVEKAEAASDALQMAATVLTGASAALAVILAMAASRKIAVPIVQLTADV
                     KRVAEGDLTVETRVKSRDEIGDLADAFNRTVGSLSVLVSDAVSSSEIVASASLQLSSS
                     MQESAEAMQQISDTVQQMATAALQGSDSAEQSAAAAEQLSMAIGQVAAGAESQTTSVH
                     EASVMLGDMGTALAKSANGLENVSSATESTKEAAAEGSAAVDSMVTSVESARVEAARV
                     SDAARELDESSREIGRIVEVISDIADQTNLLALNAAIEAARAGEHGRGFAVVADEVRK
                     LAERSLAETKAIAGLVEGLRDSTGRVVTAIEVSNAATAEAAENASGATGLLEKILANA
                     NETATYVQELAAYGNDLITASARLQKAVEEIVMVADGNAAAAEEMEAGVGEVKASIDS
                     VAAVSQENAAAVDEVSAAASEVRASIESVAESADALAKMARNLREAVSKFKVG"
     gene            complement(8405..>9000)
                     /locus_tag="PLU15_03250"
     CDS             complement(8405..>9000)
                     /locus_tag="PLU15_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO29969.1"
                     /translation="SKLSGTFQSACIARDTLERGSIEVIDTHQATMCQGFVALAAARA
                     VTAGAGPDGPRDAIYRGISSARLFFTVDTLEHLVRHGHIGKAASMLGTMMSVKPIVSI
                     IDGEVTPVAKAYGRGSILPRVIELMGEKIGPAQPIHAAMIDADSADRADEQRVKIEFL
                     FSCREIIMTECGPVVGTHAGPGAIGVAWLPCGGADLE"
     assembly_gap    9001..9100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9297..10307)
                     /locus_tag="PLU15_03255"
     CDS             complement(9297..10307)
                     /locus_tag="PLU15_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOO29970.1"
                     /translation="MRGYIGVDVGSVSTNLVLIGDDDVFIDSIYLRTRGNPIEAVKDG
                     LRMLRTKHSEFEVLGAGATGSGRKLAGVIVGADIVKNEITAHAVAAMSAVPGVQSVIE
                     IGGQDSKIILLRSGVVVDFGMNTVCAAGTGSFLEHQAQRLGVAIEQFGDLACASQSPV
                     RIAGRCAVFAESDMIHKQQVGHSLPDIVSGLCEALVRNYLNNVAKGKQILAPAVFQGG
                     VAANIGMRRAFERALGYEVVVPEHFDVMGAIGAAMLARRHIESSKRSTLFAGWEVPEQ
                     TYTTSSRECSGCPNMCEIVEVARNSAVVARWGGRCGKWEDMRRADHENCDRDRQHSGS
                     PC"
     gene            10886..11287
                     /locus_tag="PLU15_03260"
     CDS             10886..11287
                     /locus_tag="PLU15_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO29971.1"
                     /translation="MANILIVDDDPDIRGVFQEALELLGHCVATACNGLEAIGLLSCD
                     PLPDILLVDVMMPVMDGRELLGRIGAAPRTSGLPVILITGASPDSDDFPNACDYQALI
                     TKPFDIWDVAKCVDSLLEDCKPQIDRSSPES"
     gene            11561..14401
                     /locus_tag="PLU15_03265"
     CDS             11561..14401
                     /locus_tag="PLU15_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29972.1"
                     /translation="MRRSIVAHRRSRQNPHPALPVVMIAFLLCAGVALAHSPSVHASA
                     EQCLAATAKQDASSAHIRWWTLKTEHFNVHFHDGLADVAQEAAFLAEHAYAILKQEFG
                     EAQNVIDLVITDQVDATNGFTLPVFDCITIYTANLRWSDVGNTKNPSWLEALLFHELV
                     HEIELNQVRGGARLARLLFGKIVMPNSVKPIPFVEGLAVYEKYKALGESRVSDSRTRM
                     MARQMALDDAFPTFEQISCFYKRDLWPPSQLLGYNFGALFMKYIEDAYGDRALADIAD
                     IYARGFPLGGSFNRSVRKALGVSIAELYDGFIQALRGEFLAEAESIAAEGLTPAIRLT
                     SLGGVVESPSWSKGTGLAAYANYSSIRNGVRLIDPATGADREAFACIGYDGAFPGLSA
                     DGEWIVYSKPQFNAGPYLERDLYVRNLRSGKETRLTKGERAYYARFSPDGNQVYYARI
                     DGHDGSSALCAVDMATGTTRAIRSFADWRGAIHSFEPSPDGQSIALSIWRPGGYQDIY
                     LMSQEGSNIVQVTSDLAQDCDPTWTPDGRHVLFSSDPDGVYNIYAADVETGEICKVTN
                     VLSGAFFPSVSPDGSLLLFVGYGKDGYDLLLTDLDIASARPAPRKHDPIPDLPPRQAW
                     EYEITPYMPLAHMAPTFWVPNVTAEGVEALVLGVEPVGLRQYVAGVGLDLANGGPTCQ
                     LAYMDQQNARFPLSLAMSGGPKGNSQSVAISFPMWGDPSWSGGLTIEYAHAGLTGTHY
                     HLLAADGDLSHIVGRDLLRSTATLQAQAALTSSTFAGPWTPEISVTLANSLRLPMTRN
                     NRFEVQAVAGWTGSESYADMLCIGGHDGKYPVRGVEHAYSSGTHIMLGSAEYEFTLAP
                     VEASFGPTSIFIDDLQASVFIDAGSAGPCFTLENFVASVGMEVGASIVPGYTSGRGLI
                     SLGLAKPIVWRGTAPEPQSDFTIYVKLSGTPL"
     gene            complement(14457..14915)
                     /locus_tag="PLU15_03270"
     CDS             complement(14457..14915)
                     /locus_tag="PLU15_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOO29973.1"
                     /translation="MMPEEFFELLRPHAEKVFEETGIPVEIMLAQAALETGWLKNPIR
                     DKYTGKEAFNLFNIKGEGPAGSVMANDIDYIKGKKVHVEAEFRAYNSYEESFADYASL
                     LTEAQRYAPALAVRDDPVAFAWELQRAGYATDPNYANKLISIMQRHMGVT"
     gene            complement(15132..16148)
                     /locus_tag="PLU15_03275"
     CDS             complement(15132..16148)
                     /locus_tag="PLU15_03275"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOO29974.1"
                     /translation="MKARRRIVGMLVVGLILVALAPTTVAIEALHYRLVGEVNGKWSV
                     QMDLVVDGAQVSGEYWYDAYGIPLVLDGVNRGGSLIVKERTADGKVSGEWAFDFNPFV
                     TEWKGTWTSADRTRRYPFSLRLLADMVQTQEVRRGFIRVWLERPLFAEAKSNRSMAVV
                     QEIVDSQLWSSVDEDFTFFPEMYPDYCRAGMYWEHVDHVTIRYASNSIISMMHETYGY
                     AGGAHGMTWYTPINLRLSSSGASGFGLDDVFLKGSGWEKKVSELIIADLKKQKAGWIT
                     RGEVTEVGVDLLDRFTVSPAGIDFYFGPYEMGCYAEGSYVSFLPWSALKGFVDTSGPA
                     GFLK"
     gene            complement(16323..17732)
                     /locus_tag="PLU15_03280"
     CDS             complement(16323..17732)
                     /locus_tag="PLU15_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO29975.1"
                     /translation="MYDVIVVGGGSAGLWTAKTAAENGCKVLLIDKRAKDDIGRKISY
                     DSTPPYVFEQLGIPLPSGKELDDTGDVLTVISPNKKHRVEAAVPNVLFSRRLLGQRLA
                     GYAADAGVEFMYRTEVVGPVIEDGYVVGVRARHLDSCDEEEIRARIVVDASGFYAVIR
                     RKLPPEILVQDPQYCEDVVLGYREVRRIVGESNDCPPPEYRGYYDYLGYHGGYLWIVR
                     EENNWANIGIGVQDYPGNPGPVRPVQEFADDNPAIGPECMVRGSGDPMYIPLRSCQPQ
                     LSANGFMMVGDAACQVRPSTGYGWHTGLLAGDIAGKVAAEAVKKQDVSREALWDYDVG
                     WKRGLGAILASFDAIRIMCQSVPNDELDFLLAKGILGSEQIEALWNDKPFEVGTGKQV
                     SSFFAGIGHTGLLLKVKSVIDSADKLYKLFLEAPDSPEGFEEWNRERVEIFTNLRAKL
                     GVKVHEKDYFGEGMKHELK"
     gene            complement(17800..19032)
                     /locus_tag="PLU15_03285"
     CDS             complement(17800..19032)
                     /locus_tag="PLU15_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOO29976.1"
                     /translation="MGKKTCCTENDPFFIDYPGRIKSIQEAMANEGFDVYLGSRLRTI
                     SWTTDAFCPWRSYIVIPREGLPTLFTFVIDASRVADDTWLPEDNVRGYAPLGGQDQIT
                     QISDFIKDELKIRKGRLAYETGLSNYTPEGNLSQYEYEMFTAALPGLEFVNAHIIVDR
                     LSLIKDEGTINRFREASRYTDVGHEAVREALVNGGWKGKTETEIAGIAECAMRKAGSE
                     WAWSFTAGNEIASGYRTGYAGGACTPATRRELQPGDSLMVDLHAMYRLGLGDYAYNYL
                     IAPVTDRMRWHARNYIDVVQMCIDNYREGITPLGLASMVMEMAEERGFTDYVVPGFEH
                     GIGMMGDEWRIGLNTGPIKYWTDPNHVYQAGEMLICAVQYACPEEKIGFRYECPILIR
                     ENCCELMAEFPLDVEEID"
     gene            complement(19049..19450)
                     /locus_tag="PLU15_03290"
     CDS             complement(19049..19450)
                     /locus_tag="PLU15_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOO29977.1"
                     /translation="MEDKLKYLSPEWRDEAYRRLSAELDPEKMNYATTSLVSVYHNCP
                     DGKERYFFVSCADGAIGDVSVGEGEPPEAEFSISGDYDVFAKMSRGELGAQAALMSGK
                     LKLKGNMVKALKLSSLADRLNRVIATIPTQF"
     gene            complement(19499..20092)
                     /locus_tag="PLU15_03295"
     CDS             complement(19499..20092)
                     /locus_tag="PLU15_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO29978.1"
                     /translation="MKEQERNDEVMKAAFEVFAEYGYRKATLDDIAQRLGLTKSALYL
                     YSDGKRDLYEKCVGRALIEWQSYVADAVAKEENVCRKFVALCQSSFDWLLNNRDMQMI
                     LSRDPNVFPLFGEDDPYFEINQKSIKMLDDVLERGVAEGRFRPIDREVVTNLLFWIYK
                     MVIIGTCVTPDAVDVRRLINGGLDVILHGVFVPGECG"
     assembly_gap    20306..20405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20526..21353
                     /gene="nadE"
                     /locus_tag="PLU15_03300"
     CDS             20526..21353
                     /gene="nadE"
                     /locus_tag="PLU15_03300"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOO29979.1"
                     /translation="MDCKAVADVICDWLKENTLRAGCSGIVFGLSGGLDSAVMAGLGM
                     RAMPEGCLGVIMPCHSDPQDVEDADLVARYFNLPTITYDLGPIYDEHLANLQRLLDPY
                     VAAKDRRRLHAEDADSLPDGTGESASRLESSKVRMAEANLKPRLRMTALYYYANVLGY
                     LVAGTTNRSELTVGYFTKYGDGGSDLLPLGNLVKTDVRELAAYLGVPQSIIEKPPTAG
                     LWSGQTDEGEMGISYADLDRYILTGEASDEVRDRVEALNRSAQHKFVRPPIAPIPSR"
     gene            21551..22513
                     /locus_tag="PLU15_03305"
     CDS             21551..22513
                     /locus_tag="PLU15_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOO29980.1"
                     /translation="MQKALPATLAVMCIGALMMIGLSLSPPRSSAPIKPIANLAERIR
                     DAAVIAISTLAVLFIGTVLAFVIIERQQSGGKRIFRAPTHVPGWVRILSLALVMLAFV
                     AATYTPPTEHEKPTPGPEAKGGEKKAPAESVEEPESEYAPRPEPPLVVPAKVAPWPWW
                     KYGLIFAAIAFIAVIGVAVIGPQRTAVTPKPVASDALAIADMLSIEMVDFSDAAIEAI
                     EEEPDAQIAVLMCYLAFLSALEMGGAPAESHYTPEECALNAEKAFNVPRRSLSGLLAA
                     YQKAFFSTHDVTSQDKAAALASLRSLKSHIDSISVAQAASGQRG"
     gene            22520..23026
                     /locus_tag="PLU15_03310"
     CDS             22520..23026
                     /locus_tag="PLU15_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29981.1"
                     /translation="MSSRFDEDIRHKELVSFVEMILTLAAFALFSIALYLLMSPHKRP
                     LVPKISGVGCLYIACLRAWQGAKQGRENPMPWLVTQFNFWEWVDSWAPWRKRRASSPA
                     VCPQFDAFRRKVRLSQGRSRYMVRFLEEQEAPVDLIEHVKGGYHGNGERMTPDDISSV
                     LDVMEELL"
     gene            23038..24003
                     /locus_tag="PLU15_03315"
     CDS             23038..24003
                     /locus_tag="PLU15_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO29982.1"
                     /translation="MIIQASAKCHEIISSVSAVIVGKDDELQFVLMSMIADGHLLLED
                     VPGVAKTLTAQCFAQALGLNFKRIQFVPDLLPGDITGSTIYDARREDFKFKPGPIFAN
                     LILADEVNRGTPKTQSALLEAMQERQVTVEGQSYVLDPPFLVIATQNPLEFEGTYPLP
                     EAQIDRFIARLRLGYPSPEAEREILSRRQDRRTDRAVARQAIEKSEFLQIQNAVEHVY
                     VSAEIQDYIVSITTETRNDRRVQLGASPRGSLALFKLARARALMYERDYVVPDDVKAV
                     AVPALAHRILLRPELWAERITGESIVEDALDRVPVPVADWARAVD"
     gene            24022..25338
                     /locus_tag="PLU15_03320"
     CDS             24022..25338
                     /locus_tag="PLU15_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO29983.1"
                     /translation="MGSRLTEKGYVYIVSFGALVLASILLDRWELLLLASVFMFGLFA
                     VAYEQDEPEYSISVNVSHDRCFEGEQVLLEVSISSGSDIPLLEAHILLCDEENHVISS
                     RQLSLSMRAGEIRNFTFRVNMPARGMFYVGDTVSRSYTRSGLKSFGDRVWPGEACLVY
                     PRPEPIRVDWGLAARSRQHVGECPSRVPGEGLEISDIRAAMPGDPVKRVNWRATLRFG
                     KLYVNDSTRDRNIDVVIVVDGLADVGTSPSTYLDCASRAAASIAMGFLENRDRVGIVR
                     YAGAVDWAVPRPGRNQLYVILDRLARLYAVQSFVAPNMRLLPRSVLPPGSLVLVITPL
                     LDDRAIDMIVGLAARGCNPMVLYVSPIPFIEDQLKDSAEDQLAKRWWGLDHKAKLKKL
                     RALGLQVAEWDGNGSLDACLSHYFGGQGFRSAGRSPWQSASRGGWA"
     gene            25335..25964
                     /locus_tag="PLU15_03325"
     CDS             25335..25964
                     /locus_tag="PLU15_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29984.1"
                     /translation="MRKIDWLRWLPAFLCLLVVICVVGITPTVYVMIPAAAALALSLW
                     GTVAANFKPFGWSSACVLTACALSLMEKRPVAWFEPALMGLGVICFLGAAQINSVYQR
                     KDVSPDLMEGFEDKAACLEENDAQAEQPLIDSAPAVRSALAIGAQRAVAIVFAAMAFS
                     YMFFALAGVRAKQLEEGFFMMMLGTTVIGVSIYWLIRLAAPDAYKKPHR"
     gene            complement(25918..26937)
                     /locus_tag="PLU15_03330"
     CDS             complement(25918..26937)
                     /locus_tag="PLU15_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOO29985.1"
                     /translation="MAKCVRGRGAQWVGLPGAWYHDNDIVGLGGPGLIISASRRTDIP
                     AFYGEWFMNRIRAGFCLVPNPYCPTKVSMVSLAKDDVDAIVFWSRDPRSMMPHLHELD
                     NMGYGYLFQYTINGYPRLLEPKMPDLGSAIATFVELASVIGPSRTFWRYDPIILSNIT
                     PPEYHIKMFSEIAAALEGSTMRVTVSLLDLYRKTKRRLEELEEISLWETEVPGAPGQL
                     KSDVLGLFAEIAAIARSHGMVITSCAEPYDLSACGIGHGACIDADTIRAALGVEVSRR
                     KDPGQRGECGCVVSRDIGVYDTCMRGCVYCYATDGTGTEKAHDPHAPALNGGVSCTRQ
                     ARLSG"
     gene            27104..29146
                     /locus_tag="PLU15_03335"
     CDS             27104..29146
                     /locus_tag="PLU15_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO29986.1"
                     /translation="MVSDGSSADIESKLSAEEIRYMLHGSDSELVLNSLPVGVALLRM
                     PGYILEMANSTFARMIGEVKSHSLAPESRFEEVLPKQWMNVIIPLVDRAIETKNRVTV
                     CKARSGAEEGGSDAYWLIACQPVPNGASNTHHAIILSQDVSEQSVLLKRLKALSALGS
                     SLSRGYSLDEFLADGVGKCSAAISADYCAAFLLDQDTPRLLRVAESKPLCWLPSEITF
                     DSPEEWPCGIETSASAYVSLPQANAREARWLGSIGAYGYLRIPLMTVGRPIGVMAAVF
                     KKIGYVPPQEDIEFAELVAERCAMAIAADAAIAEYGRLLAAEQEAHRAATAKAAQMEA
                     LLESLECGVVAYDRNGYVILTNRACEEMLDVGKPQSRYGDDIAFGITLEKLDGEAVSL
                     KEMPYCKLLREAALADQHYMMHYTDGSYRVISFNGGIVRDAKGQMSSAIIIMRDITRM
                     YEMEKTREEVIRLISHDLRSPLTLILARAQMMERVADKPDAVRKNAMGIVLSARYMNI
                     MISDLSDSFRIQSGKITLKRQVFDLAKFISDCVERWLDAPETTRLKLDIEPNLPPVYA
                     DPVRLERILTNLTTNAFKYSTGDSEVLVEAARYQDSDDQALISITDHGQGIAEDELPH
                     IFERYWRASEARERSEGMGLGLYICKELVEAHGGRIWVHSVQGQGSTFFFTVPFAG"
     gene            complement(29405..30634)
                     /gene="grdD"
                     /locus_tag="PLU15_03340"
     CDS             complement(29405..30634)
                     /gene="grdD"
                     /locus_tag="PLU15_03340"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HOO29987.1"
                     /translation="MTDDAKRRIAELFEKLADALESGADLAGLAEGAGGRRPRIGLTV
                     LGSEHGPEEIIDGALRALRQYSDLEVALIGPSELAESVRGVAGLRLYPAASEEEQHRE
                     MERLLDAGEIDACVTMHYNFPIGVSTVGRVVTPSRGREMFIATTTGTSGTERVENMVK
                     NAVYGIVAAKASGVVEPTVGVLNIDGARQVERALRKLSDAGYPIQFAESARADGGAVM
                     RGNDLLMATPDVMVCDSLTGNLLMKIFSAASTGGDYEATGYGYGPGIGEGYGRIIMIL
                     SRASGAPVVAGAIRYAADLVKGGVHEVARVEFAAARKAGWPEIGLPTGAGASASAPAQ
                     GDAAAAAESVEPPPKKVVTSDIAGVDILQLEEAVSVLWGAGIYAETGMGCTGPVIMVA
                     DEDKDAARKALESSGYL"
     gene            complement(30645..32207)
                     /gene="grdC"
                     /locus_tag="PLU15_03345"
     CDS             complement(30645..32207)
                     /gene="grdC"
                     /locus_tag="PLU15_03345"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HOO29988.1"
                     /translation="MDFAVVRGVSYVLVHAPSMLVHMGTTQTVEREANPDSDYLKELP
                     KHLRSYSECMAYPPNQTYIGNITPDELGNIPRPWHENPLAKASRAGARGEVMPEDEFY
                     ALMKIVDSFDLVVLEEGFTAQVAAELSKDSVWIEGDRERLGAGAPISQIEELVSSGRA
                     EGLYIGDKLVGCVKAAHERDKNLTAHVMAENLAAKASGTLALRHLVAKSGIDAADIDY
                     IIECSEEACGDMNQRGGGNFAKAIGEMAGCVNASGCDVRGFCAAPAHAMVNAAGLVQS
                     QVFKNVAVVGGGATAKLGMNGRDHVKKGLPLLEDVLGGFAVLVSANDGTSPIIRTDAV
                     GRHKIGSGSSPQAVMTAIVVDPLERVGLSLKEVDKYSAEMQNPEITEPAGAGDVPTSN
                     LKMIAALAVKRGEIARGDMGTFVKKHGMPGFAPTQGHVPSGVPSLGYLCDEISAGRIE
                     RAQIIGKGSLFLGRMTNLFDGISFLIQKNPGKCGSRDRAAAAGMAGVSEHELRKIMAD
                     ALRGAAQTLVES"
     gene            complement(32320..33633)
                     /gene="grdB"
                     /locus_tag="PLU15_03350"
     CDS             complement(32320..33633)
                     /gene="grdB"
                     /locus_tag="PLU15_03350"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(32584..32586),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HOO29989.1"
                     /translation="MTKKMRVVHYLNQFFGQIGGEDKAGIAPFDKPGHVGPGMALAAA
                     FGDEAEIVGTVICGDTYFGENMEKATEEVVALIEKYKPDLLVAGPAFNAGRYGTACGA
                     VCAAVAEKFGIPVVTGMYPENPGVDMFRKNAYIIETGDSAAGMRKAVPQMAKLALKLA
                     KGEPTDPATDGYLSRGIRENYFAEKRGSERAVEMLVKKLSDKDFVTEYPMPEFDRVEP
                     NPPIADLSKATIALVTSGGIVPKGNPDHIESSSASKFGKYDLEGVEALTSETHETAHG
                     GYDPVYANADPNRVLPVDVMRDLEREGVIGKLHRYFYTTVGNGTSVANAKKYAAAIAK
                     ELVEAGVNGVILTSTUGTCTRCGATMVKEIERAGLPVVHMCTVVPISLTVGANRIVPT
                     VAIPHPLGNPAMGHDEEKQLRRRLVERALTAMTTEVEGQTVFEEK"
     gene            complement(33730..34197)
                     /gene="grdA"
                     /locus_tag="PLU15_03355"
     CDS             complement(33730..34197)
                     /gene="grdA"
                     /locus_tag="PLU15_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198010673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(34069..34071),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOO29990.1"
                     /translation="MILDGKKVVALGDRDGIPGGAIEECVKTAGGEVVFSVSECFVUT
                     AAGAMDIENQRRVKELAEKYGAENLVVILGGAEAEASGLTAETVTAGDPTYAGPLAGV
                     QLGLSVWHIVEDEIKAEIDPEVYDEQVGMMEMVLDVDAIKEEVSSIRKQYSKY"
     gene            complement(34276..35562)
                     /locus_tag="PLU15_03360"
     CDS             complement(34276..35562)
                     /locus_tag="PLU15_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOO29991.1"
                     /translation="MRLELGRIRIKDVQFGPATKVEDGVLYVDRAAIEAIVREDERIA
                     DVTVELAKPGEEVRIIPVKDVIEPRVKVEGAGGVFPGMVSKVETVGDGRTHVLSGAAV
                     VTCGKVVGFQEGIIDMTGPGADYTPFSKLNNVVVVCEPIAGLPQHEHEVAIRMAGLKV
                     AAYLGEAGRDIEPDEVDVYECLPLLEQVNAYPDLPKVVYVYMLQSQGLLHDTYVYGVD
                     AKKILPTLIYPTEVMDGAIVSGNCVSACDKNTTYHHQNSPVIHDLLARHGRDINFIGV
                     VITNENVTLADKERSSNFASKLVEMLGAEGAVVSEEGFGNPDADLIMNCVKLEKKGIK
                     TVLVTDEYAGRDGASQSLADANPLANAVVTAGNANEVVNLPPMKKVIGHPEAANIIAG
                     GWDGSLASDGSITAELQVITGATNELGFNKLSAREA"
     gene            complement(35590..35910)
                     /locus_tag="PLU15_03365"
     CDS             complement(35590..35910)
                     /locus_tag="PLU15_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO29992.1"
                     /translation="MVQVDKVNFEEEVLQAKGPVLVDFWSTKCEPCMALLPDVEALEA
                     KYGDQIKFCKLNVLENRRLAISQKVLGLPTICIYKDGEKVAELTKDDATAANIEAMIK
                     EQIQ"
     gene            complement(35978..37222)
                     /gene="trxB"
                     /locus_tag="PLU15_03370"
     CDS             complement(35978..37222)
                     /gene="trxB"
                     /locus_tag="PLU15_03370"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOO29993.1"
                     /translation="MAAEDMKTYDVIIVGGGPAGLSAALYASRSKLSTLVLEKAKPGG
                     QAGTTEWLENYPGFPGGVKGPELMEHFAAQAREFGAEIIKDDVASVDFSGEKKLVCTA
                     GGGQYSASAVILCPGAEPRTLGLPGERELRGKGVSYCATCDGDFFTDLDIVVIGNGDA
                     AVEEAMYLTRFAESVTMIVVHDEGILDATAVIRDRAFKNPKMKWVWNSVVDEIRGDGI
                     VESVIIRNLKTNERTELETNGVFVFVGTVPNTQFLSGAVEMDKRGYIIANEQMETSVD
                     GVYCAGDARVKYLRQVITAAADGAIAAVAAEKYIHEEEGFREMVLAPAFPVMVCFWSP
                     TSQESLDFMPAVERIAEARAGSLNLVKIDIYKNQRIAGRYEISAAPSILFFNKGQVCE
                     RVQWPTTEEELTLVADRVISGK"
     gene            complement(37256..37690)
                     /locus_tag="PLU15_03375"
     CDS             complement(37256..37690)
                     /locus_tag="PLU15_03375"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOO29994.1"
                     /translation="MDSRTYRVITNNPLVAATWPGSVEWVSGSVKDVFRCVRDYVHAG
                     HVLLTHPLSGSVKPNESPYKSVIITDYPASAMVDLDSLRIIEGAMVVLHNLGAPRFTH
                     IPPRVDDDYQLIDCDLMASAIESLHDAAWNGPVEGCKTEAFQ"
     regulatory      complement(37787..37855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(37856..37988)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(38211..39203)
                     /locus_tag="PLU15_03380"
     CDS             complement(38211..39203)
                     /locus_tag="PLU15_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29995.1"
                     /translation="MSLPFTIGERFGLLAALIVAAVAVVVSPWACGAQQLACIHVAEA
                     CLTAIPSFPGICHASVLLPETVRKEFMFVQGGKVYCVELGGLEEPGDAGTESVSIIRF
                     GLGAEPENIGKIEGNAMSVWLLGDINKDGCMEMLVRRSEGHFAYKTSIVYIDQKDRMR
                     SFDISGYTDSQGSTDLFLTPESFADLDGDRIPEIVATVSPKGFPSLLGAKPPQISLVF
                     KLNGTTYSPTGQRFAEIYRFLAAQTRCELASYWDFEVVEYPQSYVRSCCELLLYHALA
                     GDADVGAEEFDKAIRILQDAGVEITEDDNRALKEFGREREAIVHEIMRGLLTGP"
     gene            39387..40592
                     /locus_tag="PLU15_03385"
     CDS             39387..40592
                     /locus_tag="PLU15_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HOO29996.1"
                     /translation="MAIGFEQGALHIKRLSLVEAVMMLVGGNVGAAILSLPYAARASG
                     YLGAVTVAVITTVFSAISHLYIVEIMLRTKSPGQLVGLVRTYMFDSRHGKLYLLLVSS
                     LTIGLVIPSLTAYVIGGGAIISSLLALPAWAGHLLFLLPGAAVVWLGLEAAGLAQGVS
                     SIAMMSVLLLFAGISMRHPSFDPARLARFSAKPLAAALPVGIFTSMSQSLVPEIVRGL
                     AHDPRSAPTAIKWGLGINLLFLLIFPMAIFGLQDPGEISEVVTISWGQALGPAIYFAV
                     NLFALLALLTSFWGGAGAALGNVVEIFRFKSEWDIGARLAAFAITITPSVVLASVGNF
                     DFVDMIQTAGSVGGVILAVLPIFILAKARKRSEREPEYQVGIAFSPIVRVALFVFYVG
                     VLIYAAFAR"
     gene            40701..41744
                     /locus_tag="PLU15_03390"
     CDS             40701..41744
                     /locus_tag="PLU15_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO29997.1"
                     /translation="MIIRSAEPRDDAVLLGIESVAGQGDQIRLIGERDSFFSRARQFE
                     NTLLFVGENETTGKLHGVMAASVLPVRVGGRERIGAYMFDLRSNPETNKGLSRAMYLI
                     WKGLEDRLLQCGTEFIYGLIKEDNSRSYSIATRMGATPRGEKRFLTFPVFRRIRVSRE
                     VQTISHVDAMEEHLAAAKHYDGPYQLWPIMDDFSYMQGLLDKYLVAKMECGSSSLKIW
                     DSSLNCAYRVSAMPRPFEILGTVLRAASHLVPLPKIPMLSQKLRIWNVYDIITRPGQR
                     SEAQALIAKANNLALEHGVDFLIITVDAEEDELRWLRRRCLMPLDYKLMVKEYNPVPE
                     FFGKTYFDPRYLG"
     gene            complement(41870..42934)
                     /locus_tag="PLU15_03395"
     CDS             complement(41870..42934)
                     /locus_tag="PLU15_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO29998.1"
                     /translation="MGKLEGIESIYYTTDRAQFHILIRGDFDAEPIVFVHGNTASATF
                     WEEVMFALPDDYRSVAPDMRGFGLTQAKPVDATRGVRDWSDDLYSLTWLLGLREFHLV
                     GWSLGGGIAMQYLLDHPGKVITLTLVAPISPYGFSGTRGPDAIPCWPDYAGSGGGGVN
                     RQFLELMKRKVNDGGDDPALPRNILNTCFWRPEFRPEREDDFLDALLQTAVGDHNYPG
                     DFAPSPNWPGYAPGTCGVLNAISPKYFRVDGIADLDVKPPILWVRGSDDLLISDASPF
                     DIGYLGRLGLVPGWPGNEVFPPQPMIEQTRAVLDRYAKNGGFFREVVFQECGHSPHIE
                     KKDAFVAELVEHMRWATTYI"
BASE COUNT         9526 a        13416 c        10635 g         9566 t
ORIGIN      
        1 tttgggattg cgcacttcat ttcagatcag ccccgcccgt ggctaacgcc tgagattcgt
       61 ggccgtggcg agcatgtcat cagaagcctg gatagccctg gagttcactt cgtacgccct
      121 ttgagcaatg atcatattga ccatctcttc gactatcttg acattggaca tctccaggaa
      181 ttgctgcgca accgtgccca tgccgtcaag gccaggcgta ccaataatag gctcgccaga
      241 agcggcagta ggcctgtaca ggttgcgccc catcgacgtc aggcctccgg gattgagaaa
      301 acgagccagt tcgatgctgc ctagctcctg aggctcattc tcccctgcga tctccactga
      361 cacagtcccg tcagttccaa tcgagatgct caccgcattc tcgggaacga cgatttccgg
      421 ctccatcaca tacccatcgg acgtgacaat ccttccgtcg gcatcgagct tgaatgcgcc
      481 cgtgcgcgtg taggcgattg acccgtcagg catgagcacc tggaagaagc cgtccccttc
      541 aatcaccatg tcaagggggt tttccgtctg aacgaagtcg ccttgtgaaa agactttttg
      601 aatcgcagca gccctggttc ccagtcccac ttggatgcct gtgggcaccc tcgacccttg
      661 agcaacagtg ctgcccgcgc tcctgatggt ctgatacatc aggtcttgga agtcaaccct
      721 gctcttcttg tatcccgcgc tgttcacgtt ggcaaggttg tttgcgataa catccatgtc
      781 aagctgcttg gcatgcattc cggtggccgc actccaaagc gccctcatca tggcaattca
      841 ctccttcttt tgtgtgggct gcctccgtca ttcagcagcc cgcggccccc gctgcgcggt
      901 ccggccgcat agattagatc ttgattttcg tccaaagcac gtccctcgcc gccccgggcc
      961 gccatcaccc aatgcggcag actaacctac ccttcccacc tcgttaatgg ccctgtcaag
     1021 acatgcgtcg tgggaaagca atgcccgttg cgcggcctcg taaacccgat aaccggctat
     1081 catctcgacc atctcccgca caacactgac attcgaaagc tccaaagccc cctggatcac
     1141 ccgaacctgt ccggttcctg ctggaatcac ctctcccctg gcggcaaaga gtcccccgcc
     1201 ttccttcgtc agcgcatccg gggacgaagg catcaccacc aaaagcctgc cgatctcccg
     1261 accgtccgcg taaaggacgc cgtccgaacc tgcaactagc tgcccatcaa cacggatcgg
     1321 accatcatct cctaagaccc tgtgaccttc catcgtaacc aagtacgaat cggcatcgag
     1381 cttgaaattg ccagcccggg tgtacctgat tccccagtcg gtctccacag cgaaaaaccc
     1441 gtcgccctca atggcaaaat cgtagggaca gtccgtattg gagaccgagc cctccgacat
     1501 gtcaaaatag gcagaagtta tagcgcttgt catcccaagg cgccccaccg gcgtccttcc
     1561 cgacctcaca accatctccg agaaagactc cccaaccccg acatcccttt tgaagccggg
     1621 ggtcgccaca ttagccaagt tgttggcgat caaggactgc ctcgtttcct gagctctcat
     1681 gccacaagcc gcagaataga tgcccctcag caaaaacatc cctcccttcc cagatcccgc
     1741 gtcaagcgtc tcagagagcc ggcacggaac ctccgatata ctgaagtggg cattaccgcc
     1801 ctacaatata cgcaagcaag atgcatgcca caggaagtca accagccata ggccagctat
     1861 atgcggcaaa caggcgctcc attaggggag cgcctttcat cttggtcatg catttatttc
     1921 tcgaactgtg caataattgc tcacttttcc ctgagcgcca cgtgcacagt gacagtgccg
     1981 aagtcagtca tgaatgggat agtaaggatt ggagtgaaag tagtcactat caattccttg
     2041 ccggtcagca aagtaggagg ggtcagcaca cagctgtacc cgttttccga aagagagata
     2101 gccgcatttc cagtcatgat attggtcagt tcagccacgg cgcttttcgc catctcgtcc
     2161 aactcttcta cggtctcccc caccaaggca gaagctatgc gaatagcaac cccttcctcg
     2221 aaggtgtata tgacctgccc ctccaactgg ccagtgacac cgatgatcgt gttcacatcc
     2281 gagcccgtaa gagggccttt cgaatggaga tcaagctgtt cacgcatgac attcatgccg
     2341 aattcctttt gaaagaccat catcaccgcc gacgcaaaag gatttataaa ttccgctttc
     2401 atacgccgcc ccccgttccg ttcactatta cttatcgacc aaaggcgcca aagactttat
     2461 accccatccc aatcaatctg gggggtgcct gatccccaca tggccccaac ggcctctgct
     2521 acatcgttca taaatggatc cctgcgccat gccgccactc tccggaaagc cgcgaggacg
     2581 tggcgctcaa cctcagacca caccatatcc tccgccccgg aaagccttcc atactcccac
     2641 acgaacgcgg ccgcgcacgc ctgcctctcc atggtgtagc ccggcctgtg gctcaagatc
     2701 cgcatgaacc cctcacccac atcctccagc ggatccccca cgcgcgtatc tccgaaatcg
     2761 atcccgcaca caatcccccg cgtgccgcaa tcgcgccagg caaccgcttc atccccgtcc
     2821 gcctgcgcca catcgatgac gaaattgcca agattcatat cccccacaag tcgcgtgcgg
     2881 ccgtagccgc cgacgccttg cggggccaat cgtaaagcct cgtggcacga gtaaagccac
     2941 gccccaagtt ttctggcaag ttccaaagtc cagtgtacat cgcgcagaac ggggccgtaa
     3001 acgtatcttc tcaaaaggac agtaggcgta gccccgcagg ccgcagggac agccacacct
     3061 gccgactcca acgccgcaag aacttcggcc tctctaaggg cacgcccctc atccctatat
     3121 atcttcgcca ccatcacaca ttcgcgcgcc ggaccgtcct cagggaggca gaggagaaaa
     3181 gccctgcttt tcttggtgga caagtcggta ataactcgca catcccaagc cctgtcgcca
     3241 agaatcggca ccagtgccct gacgacatcg ccatcgctgc agcccatagt ctactcgccc
     3301 cgccttatct ccaccctcac ttcactcccg gtctcataac ccctgagcga tgagagcatg
     3361 ccgaggatag tccgggcgac gaaatcctga acaaacggga ccatgggaat ctcttttccg
     3421 tcaacgtaga ctgcggtctt gaaaccatgg tacgccgcag gatcaatcat cttcctgtct
     3481 gaaccttccg gcacccgaga ttcgaccatg tcaacaacct ccccgatcct ggaagtcgcg
     3541 tcgagcacgg gaatcccccg ccagctcgaa atcgcggagg caacctcgcc gcatatgagg
     3601 aaagtatgtt catcgacacg ttcttctatg tcgtgctcag tgcgggcgca cactatcttc
     3661 ggaaatggcg cctcatgcgc cccttcgata atgatccagt ccgtgtcgta gtatctgaaa
     3721 agatcctcga aactgagctt gccgtcgatg atgacggcag tctcccccgg acttagcgcc
     3781 gccgcaattt tcgcacccgc ccgtctgtgc ctgtgtgtgt tcttgcccgg agtgtcgacg
     3841 gaaaacccct cccggtggat ggtcttgata gtgcccaccg catagccccg cctgacaagc
     3901 tcgcccacaa tatgctccac agtcgtagtt ttccccgtcc gatgaagccc tgagacggca
     3961 ataacccgca tagaatgacc cccatatgcc accctgatga gcgttgccgg cccatcgcat
     4021 tccgcatgga tatgtattct atagccaccc acgctttccc tacaggccca tgcacatccc
     4081 agggtttgcg gttgtcaaac ctggccagag tctttgcgtc ctttccacaa gttctacaac
     4141 cggcacccgc aactgccgcc ccacccgtac atgcccgcat caacagaaag cccaccgcaa
     4201 cacgcatcac gggcgatgca cggtagcggc aggcttttgg ccatcagact acatgccatg
     4261 tgggcgggga ggagacatcg ctatgttagc ttgaacccgg agatcaactc ctccaacccc
     4321 gccgcaatgt ccgctagcgc ctgggaagac gcctgcatct ccctgattgc cgcatcgacc
     4381 tcctcagtcg acgccgtgac ctcctgcacc gccgccacgt tttcaaagga taccgcggca
     4441 acagcatcaa tagattcctt cacctgtcct atgccgacat tcatatcctc cgcagccttc
     4501 agactactgt ccgatgctgc cacgatctcc gccatggccc tccgcacccg gtcactcgaa
     4561 ccttcgagag agcctgcagt ctccgacaac gcctcgataa gctcaacact ctcctgtacg
     4621 cctaagataa tccgattgag aacctccatg gtccgatcca cgctctcgga cacctccgca
     4681 gtggctgaat cggacccgcc gatcgcctcg gccacctttt ctgtggatct tcccagatcc
     4741 tccacgagct tggcaatggc tttggtttca gccaaagatc tctccgccag tttccgaatc
     4801 tcgtccgcaa ccacagcaaa cccgcgtcca tgctctcctg cccttgcggc ctctatggcg
     4861 gcattcagcg ccaggagatt agtctggtcc gcaatatcat ttattacctc cacaatcctt
     4921 ctaatctcct tggaactcga atcgagatcc cgtgccgcct ccgacaccct tgcagattca
     4981 cgacgggcga ggtttacact aatcgccatg tcccgcgtgg cagtgctgcc ttccctggct
     5041 gcctcgccca caccttgcgc agcggagctc acccgtttta ggccctccaa agattgtgcg
     5101 atcgatgaac gcatctcatc gaccgcagca gagccccgac ccacactatt cgtctgcccg
     5161 cgagcacaca ccgcaacccg ctccacagtg ggcatgagag cctcagcagc atttgcagtc
     5221 tccccagcgc tcctgctctg ctgttctgcg cctgccgata tctgctgggc cgtgtcggca
     5281 atctgccgca ccgaatccgc agactgttcc actgatgcag acagctgcac gcttgccgca
     5341 gccacctgcc gcgaaacgtc cagcgccttg cctacaatct cccgtaaact ttccaccgtc
     5401 ttgttgaaag cctgcgccaa atggcccagt tcatcccggg aatcgacatc ggactgaacc
     5461 gtcaggtctc cctccgccac cttgttgaca gcatgcgtaa ggcgctccac aggttccgac
     5521 acagccctcg acaccctgag cgccatggca accgcggcag cgatccctat cgccgaaacg
     5581 agactcgcaa caagtgtccc tcgcttcacg ctggccttgg aactagctat ctgtcgccct
     5641 atgtggtccc gttccgcgcg caccaatcca tcaaggatct cagccctttg ccgccttacc
     5701 ggctcgtacg tgtctgaaaa tatcgacagt gccatctcgg ggctgagccc cgccatgccc
     5761 gttacggata ctgccaccgt cgacatggac ttgccaagct cgtccacttc cgacaacgcc
     5821 tcgaccagct ccggccttgt aagacgcctg gcggcagcgc gcatagcatt ctccgtcttc
     5881 tcccacgcca catcataatc ccgctggaat ccggcattgc caggatcgat tatcaatccg
     5941 cttatggcat tcgccgcctc aagatcatac gccctgagcg attcgactag ctgaagcgat
     6001 aataactcct cgtctacgac ttgtgtcatg ttcctgtcgt aattcgcaag ctgcagatac
     6061 atcatgacat tcccaatgag gacaaagacg accacaagca aaaacgtcac caaaagctta
     6121 gccttggtat gcttgaacaa gcgcgaccct cctcagttgg cgccatgttc atccccttgc
     6181 cgaacatgga catgaccgaa gatatagact catgctaaat gaaagaagaa cctatcaccc
     6241 caccttgaac ttagatacgg cttcgcgcag attgcgagcc atcttcgcaa gagcgtccgc
     6301 cgactctgcc acgctttcga tcgatgctct cacctctgaa gcagcggccg acacttcgtc
     6361 tacagccgct gcattctcct gggatacggc agccaccgaa tcgatcgacg ccttcacctc
     6421 gccgaccccc gcctccattt cctcggcagc tgccgcattg ccgtctgcta ccattacgat
     6481 ctcttcgaca gctttctgaa gacgagcgct agcagtaatc aaatcattcc cgtacgcagc
     6541 caattcctga acgtatgtag cggtctcgtt ggcatttgcc aagatttttt cgagaaggcc
     6601 cgtagccccc gatgcattct ccgcagcctc cgccgtagca gcgttggaga cttctatcgc
     6661 cgtaacgacc ctgcccgtgg aatccctgag cccctccaca aggcctgcaa tggccttagt
     6721 ctcagcaagc gacctttctg ccagttttct gacttcgtcg gccaccacag caaatccacg
     6781 tccgtgctcc cctgctctag cagcctcgat ggcggcattg agcgcgagga ggttggtttg
     6841 gtccgcaata tcgctgatta cctcgactat ccttcctatc tcccgggagc tctcatccag
     6901 ttcgcgcgcg gcatcactga cccgagccgc ctcaacgcgc gcactttcca cactagtcac
     6961 catggaatcc acggcggcac ttccttcagc cgcagcctcc ttggtgctct ctgtggcaga
     7021 actcacattc tcaaggccat ttgcggactt ggccagcgcc gtccccatgt ctcccaacat
     7081 cacagatgcc tcgtgcacac ttgtggtctg cgactcagcg cccgccgcaa cctgcccgat
     7141 agccatgctc agctgctctg cagccgccgc ggactgctct gcgctatcac tcccctgcaa
     7201 agctgcggtg gccatctgct ggaccgtgtc ggatatctgt tgcatggcct ccgcagactc
     7261 ctgcatcgac gatgacaatt gcagactcgc acttgccaca atctccgatg aactgacggc
     7321 atcgctcacc agaacgctca acgaacccac cgtcctgttg aacgcgtccg caaggtctcc
     7381 gatttcatcc ctcgacttca cacgggtttc aacagtcaga tccccttcag ctacacgctt
     7441 cacatccgct gtgagctgga ctatgggaac tgcgatcttc ctcgatgccg ccatcgccaa
     7501 gatgaccgcc aaggccgcgc ttgctcctgt caacacagtc gccgccatct gcagggcatc
     7561 gcttgcggcc tcagcctttt ccactgcttc ttctatcttt ctttcctgca aggagacgaa
     7621 ttcatccagg acctgttccc gccttaccct cacagggttg tattcttcag taaatatcgc
     7681 tgcagcttga atccgatcaa aggaagcgca atcgatgatc ttccgctcaa ctcgttcacg
     7741 ctcctcgttt cctgcggcaa tctccgctgc aatctccttc tcccttcccg acttgaatcg
     7801 gcttgtagcc tcccgcgtcg cggtctccat cttcgtcaac gcatcttcat aggcagcggc
     7861 aaaaatcggt tcatatggat cagagattat gccgcaaacc gcgttcgata gcatcaggtc
     7921 tgcagttctg agcgattctg cagttttcaa acccattatt tcctcagttg caaggcggcg
     7981 aacctctact tcgtacctct ccagcccgaa gtagagcaac cccgtggcga ttcccatcac
     8041 cactgcaacc gcaaggaagc ttgccagaag cttcccccga atcttgctga acatgtgttc
     8101 tatgcctccc tatgcaaaag cacaatgtca aacattaaaa cttatcgatc tttagtatta
     8161 tcggcatgcc aaagcaaact tttacccgca gtgcacacgg gccgttatcc gcagcgatac
     8221 accgaaagtt ctgacccgtt tcatcaccct ccgcccaata cagaagaccc ggcggtgcaa
     8281 cgtgcgatcc tcatccctta aatgagaatt gatctatccc ccaaatcatc ggcgctgagg
     8341 gattccatct gcgtgatcgc cttgtctgga gtttaaaacc tggctgtcct aaaaacgctt
     8401 aagcctattc caagtctgct ccgccgcagg gaagccatgc caccccgatg gccccggggc
     8461 cagcatgtgt cccaaccacc ggaccacact ccgtcataat gatctcacga caactgaaga
     8521 ggaactctat cttcacgcgc tgttcgtctg ccctatcagc ggagtctgca tcgatcatag
     8581 ctgcatgtat aggctgcgct ggtccaatct tctctcccat gagctcgata acacgtggca
     8641 agatggaccc ccgtccgtat gccttggcca cgggcgtcac ctcaccgtct atgatgctga
     8701 caatcggctt tacagacatc atggttccta gcatggacgc tgccttccct atgtgtccgt
     8761 gccgcacaag gtgttccagc gtatcaacgg tgaaaaagag ccttgcagag ctaatccccc
     8821 ggtatattgc gtctcttggg ccatccggtc ctgcccctgc cgtcacagcc ctagccgcgg
     8881 caagggctac aaacccctgg cacatggttg cctggtgggt gtcgatcacc tctatcgatc
     8941 ccctctccaa cgtgtccctg gctatgcagg cggattggaa ggtcccgctc agcttggaag
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aatcgtactc cctcagcagc
     9121 cgatcataga gcgagatgaa gctggctggt gaaggttgcg acgtagaagg tgccgcatcc
     9181 gaaactttaa gtctcgcgaa gaactcccga gatgaaaggt ctacgccatc gaggaaggat
     9241 ttctccccga agatcacatg gaggggaacc acgaaaatcc cgtttcactg gccacatcag
     9301 cagggagatc cgctatgctg tctgtcacga tcgcaattct catgatccgc cctcctcata
     9361 tcctcccatt ttccgcacct tccgccccac cttgcaacaa cggcggagtt acgtgcaacc
     9421 tccacgatct cgcacatatt tggacaccct gagcattccc gggaggatgt tgtataagtt
     9481 tgctccggaa cctcccatcc cgcaaagaga gtgcttcttt tggacgattc gatatggcgc
     9541 cgggccaaca tcgccgcgcc tatcgccccc atcacatcga aatgctcggg caccaccacc
     9601 tcgtacccca gcgcccgctc gaaagccctc ctcatcccga tatttgcggc aactccgccc
     9661 tggaaaacgg cgggcgccag aatctgcttt cctttcgcca cattgtttaa gtagttgcgg
     9721 accagcgcct cgcagaggcc actcacaatg tccggcagcg aatgaccgac ctgttgcttg
     9781 tggatcatgt ccgattcggc aaaaaccgcg caccttccgg caattcgaac cggagactga
     9841 gaggcacacg caaggtcgcc aaattgctct atggccacac caagacgttg cgcttggtgt
     9901 tcgaggaacg aaccagttcc tgcagcacat acagtgttca tcccaaaatc aacgactact
     9961 cccgaacgca agagaatgat cttcgaatct tgccctccga tctcgattac gctctgcacg
    10021 ccaggcacgg cagacatggc cgccacagca tgagcagtta tctcgttctt cacaatgtcg
    10081 gcgccgacta tcacaccggc aagttttcta ccgcttccag tagcgccggc cccaagcact
    10141 tcgaactcgg aatgcttcgt cctcaacatc cggagaccgt ccttcaccgc ctcaatcggg
    10201 tttccgcggg ttctcaaata gatcgaatct atgaagacgt catcatctcc gatgaggact
    10261 aggttcgtac tgaccgatcc aacgtcgacg ccgatatagc cacgcatgtc gcataccccc
    10321 tacctactag aggttgccca gcattatgag ggcgcattcg ccctaaccca tagccgccac
    10381 agcaagtcct ctcaatagct tcatcctcca gtatggcact gggttcgttg tttcgcgtcg
    10441 tcaaccgcac acagccaaaa accagagtcg aaacctcgac ccggatcgtc cgaactatct
    10501 acgctccacg atcgaccacc cctttctgta tcaaactacc gtgcaacgaa aaaacccggc
    10561 atcaaagacc ccatgccttc tgcagtgagc aacagcacgt gttaacgcac gtctccatag
    10621 aaaacacgat ttacctcgaa tgatgccgat atatagcggc aaaaggtaag aggttcgaag
    10681 agagcttcgc cgaattatct ccataagttg cgatgctcac acaatccttt ggtgcttcca
    10741 gagcctggaa ccgtctggcg tgtgccacca taaatccgca agacagaaca tgacaatgtc
    10801 accccagagg aaaaccaaat cgcgaggaat ccagagttgt gaacccagtc tttcatatca
    10861 ggattgcaac gaggagggat ttgctttggc caacatccta attgtcgacg atgacccaga
    10921 tatcagagga gtattccagg aagcgctcga attgctcggt cactgtgtgg caacggcatg
    10981 caacgggctg gaggcaatag gcctactgtc ttgtgatcct ctccccgaca tcctgcttgt
    11041 cgacgtcatg atgcccgtga tggatggcag agagctgctc ggcaggattg gagccgcgcc
    11101 tcgcaccagc ggattgcctg taatcctcat tacaggagca tcacccgaca gcgacgattt
    11161 cccaaacgcc tgcgactatc aagcgctcat tacaaagcca ttcgacatat gggatgtcgc
    11221 aaagtgtgtc gactctctct tggaagactg caaacctcag atagatcgat cgtcgcccga
    11281 atcgtagaac tcagactatt gactgtgcag actcttcgtt gggggctggc cctcccggcc
    11341 ggcccccaac tggtagaacc gcacaccatt ttgtgctcgc ctttcggatc ccactgcaag
    11401 cgccaacctc ctcccctctg gcaactgact cgcccgaagc tggaaggata atgacttcga
    11461 ggggagtaat catcataact aggccatatt tcctaagata acgaaagctg gggagatgtt
    11521 gcgctcagcc agcgcgccat gccccgggga ggtttctgcc ttgagaagat ctatcgtagc
    11581 acataggcga tcacgccaaa atccgcatcc ggcgcttccc gtcgtcatga tcgcattcct
    11641 tctttgcgcc ggagtagccc ttgcccactc accttcagtc cacgcatctg cagaacaatg
    11701 cctggcggcc accgccaaac aagatgcatc ctctgcacac atcaggtggt ggacgctgaa
    11761 gactgagcat ttcaacgtcc atttccatga tggactcgca gacgtagctc aggaagcggc
    11821 cttcctggcg gagcacgcat acgccatttt gaaacaggag ttcggggaag cccaaaacgt
    11881 cattgattta gtcattaccg accaggtaga tgcgacgaat ggattcaccc ttcccgtgtt
    11941 cgattgcata accatataca ccgcgaacct gagatggtct gatgtgggaa acacaaaaaa
    12001 cccttcctgg ctcgaggccc tgctctttca cgagcttgtc cacgaaatag agctcaatca
    12061 ggtccgaggg ggcgcacgcc tcgccaggct gctttttggc aaaatagtca tgccaaactc
    12121 cgtcaagcca atcccgttcg tagaaggcct cgcagtctac gaaaaataca aggcccttgg
    12181 cgagtcccgc gtatccgatt cccgcacgag gatgatggcg cggcaaatgg cgctcgacga
    12241 cgcttttccc accttcgaac agatcagctg cttctacaag cgggatctat ggcctccgtc
    12301 acaactcctg gggtacaact tcggggcact tttcatgaaa tacatcgaag atgcctatgg
    12361 cgatcgcgcc ctggccgaca tcgccgacat atacgccaga gggttcccat tgggcggcag
    12421 cttcaaccga tccgtccgca aagccctggg cgtcagcata gcggagctgt acgacggttt
    12481 catccaggct ctccgtggag agtttttggc cgaggcagaa tcgatagccg cagaagggct
    12541 cacccccgcc atccgactga cgtccctcgg cggagtcgtg gaatcaccct cctggtcgaa
    12601 aggaacaggc cttgcggcct acgcaaacta ctcttcaatc aggaacggag tgaggttgat
    12661 cgaccccgca accggcgcag accgggaggc tttcgcctgc ataggctatg acggcgcatt
    12721 ccccggcctc tctgcagatg gcgaatggat agtctattca aaaccacagt tcaatgcagg
    12781 tccctacctc gaacgcgacc tgtacgtccg caacctcaga tcaggcaagg agacgaggct
    12841 caccaaggga gagcgcgcct actacgcacg gttcagcccc gacggcaacc aagtctacta
    12901 cgccagaata gacggccacg atggcagctc agccctgtgc gctgtggata tggccacggg
    12961 aacaacccgc gccatccgct cttttgcaga ttggcggggc gcaatacact cattcgaacc
    13021 atcccccgac ggccagagca tagccttgtc aatatggaga ccagggggat accaagacat
    13081 ctacctgatg tcgcaggagg ggagcaatat cgtgcaggtc acgtcagatc tggcgcagga
    13141 ttgcgacccg acatggacac ccgatggacg ccacgtcctc ttcagctcag accctgatgg
    13201 agtctataac atatacgcag ctgatgtcga aaccggcgaa atatgcaagg tcaccaacgt
    13261 gctctcagga gcgttcttcc catcagtctc tcctgacggc agccttctcc tttttgtggg
    13321 gtacggcaag gacggttacg atctcctcct cacagacttg gacatcgcat cggcgcgccc
    13381 cgctccccgg aaacatgacc ccatccccga ccttccgcct cgccaggcat gggaatacga
    13441 gataacgcca tacatgccgt tggctcacat ggcgcctact ttttgggtcc caaacgtcac
    13501 tgcagagggt gtggaggcgc tcgtcctcgg cgtcgaaccg gtggggctca ggcagtacgt
    13561 tgccggcgtg ggattggatc tggccaatgg cggtcccacc tgccaacttg cctacatgga
    13621 tcagcagaac gcacgtttcc cgctgagctt ggcaatgtca ggtggcccga aaggcaactc
    13681 gcagagcgtc gctatctctt ttcctatgtg gggcgatccg tcctggtcag gcgggctcac
    13741 catagaatat gcccatgcag gcctcacagg aacccattat catctcctcg ccgccgatgg
    13801 agacctatcc catattgtgg gacgcgatct cctccggtcc acagccacgc tgcaggcaca
    13861 agccgcactg acctctagca catttgcagg gccatggaca ccggagatat cggtgacact
    13921 ggcaaactcc ttgaggctgc cgatgaccag gaacaaccgg tttgaagtcc aagccgtggc
    13981 gggatggact ggttctgaaa gttatgcaga catgctttgc ataggaggac atgatgggaa
    14041 gtatcccgtc agaggagtcg aacatgccta ctcatcggga acccacatca tgctcggcag
    14101 cgctgagtac gaattcacat tggcgccggt tgaggcaagc ttcgggccga ccagcatctt
    14161 catcgatgat cttcaggcat ctgtcttcat cgacgcaggt tcagccggtc cgtgtttcac
    14221 attggaaaac ttcgtggcca gcgtgggcat ggaagtggga gcttccatag ttccgggcta
    14281 caccagtgga aggggactta tctctttagg cctggccaaa cctatagtat ggcgcgggac
    14341 ggcgcccgaa ccacagtctg atttcacgat ctacgtgaag ctgtcaggca ccccgctata
    14401 gcaaaccccg atatcgaggc gcgcgacagg tggccctgct gcgcgcctgc tgttggtcat
    14461 gttactccca tgtgacgctg cataatcgag atgagcttgt ttgcgtaatt gggatcagtt
    14521 gcatagcccg cgcgctggag ctcccaggca aacgcgactg gatcatccct tactgccagg
    14581 gccggagcat aacgttgcgc ctccgtaagg agggacgcat agtcggcaaa tgattcttcg
    14641 tagctgttgt aggccctgaa ttcggcttcg acgtgaacct tcttgccctt gatgtagtca
    14701 atatcgttcg ccataaccga acccgcaggt ccctctcctt ttatgttgaa aaggttgaac
    14761 gcctcttttc ccgtatactt gtcgcggata gggttcttca gccacccagt ctcgagagca
    14821 gcctgagcca gcatgatctc caccggaatc ccggtctcct cgaaaacctt ttcagcgtgc
    14881 ggcctgagca actcgaaaaa ctcctcaggc atcatgtcac cattgcacct ccttcagcct
    14941 catcacagcc aatctatgaa acaagaccgt caaagtcgac tgccatcagc accaattcgc
    15001 cgaggcatac gcgatcagca cggtctgcca ccccatacta ttccatttta cggcaagcgc
    15061 accagcagct ccacaggcgc aaagctgcat caagaaacca gcatacgaca acgcctcatc
    15121 ccatcgcttc tttattttag aaagcccgct ggccccgacg tgtcaacaaa ccccttcaga
    15181 gctgaccaag gcaggaagga aacatagctt ccttccgcat agcaccccat ttcatacggg
    15241 ccaaagtaaa agtcgattcc ggcagggctc acagtgaacc tgtcgagcaa gtccacaccg
    15301 acctcggtca cctcgccacg cgttatccaa cccgctttct gcttcttcaa gtccgcgatt
    15361 atcagctctg agaccttctt ctcccaaccg ctgcccttga ggaacacgtc atcgaggccg
    15421 aatcctgacg cgccggaaga cgaaagccgc aaattgatag gcgtatacca cgtcatgccg
    15481 tgggcgcctc cagcataccc gtacgtctca tgcatcatgc tgatgatact gttcgatgcg
    15541 tacctgatcg ttacgtggtc tacgtgttcc cagtacatgc ctgccctgca atagtcgggg
    15601 tacatctcgg gaaaaaacgt gaaatcctca tcaactgagg accagagctg actgtctact
    15661 atttcctgca ccactgccat gctcctatta gatttggctt ctgcgaataa cgggcgctcc
    15721 aaccacacgc ggataaatcc tcttctgacc tcctgagtct gaaccatgtc cgccagaagc
    15781 cgaagggaga agggatatct ccttgtccgg tcagcactcg tccaggttcc tttccactcg
    15841 gtgacaaagg ggttgaaatc aaaggcccac tctccggaaa ccttgccatc agcagttctt
    15901 tccttaacga tcaacgatcc ccctctattc accccgtcga gcacgagcgg gatgccgtat
    15961 gcatcatacc aatactcccc tgacacctgc gcgccatcga caaccaagtc catctgcaca
    16021 gaccattttc cattcacctc gcccaccagg cggtagtgaa gagcctcaat ggcaacggtt
    16081 gtaggtgcca acgccaccaa tattaacccg acaactaaca tcccgacaat cctgcgccga
    16141 gccttcatct gtctctcctc caatcgtagt atggggccat tatcgcacgc cgtcctcaag
    16201 gctccgcctc gtcagataat gggcaacgcg gcagagagca tgtcccctgc cgcgttgccc
    16261 agcgaaggta ggttcgcatg acgccgttga caggcattgc cgggcgtctt tccacctgct
    16321 gttcatttca actcgtgttt catgccctcg ccgaagtaat ctttttcatg caccttaacg
    16381 cctagtttgg ccctgagatt agtaaatatc tctacacgct ctcggttcca ttcttcaaag
    16441 ccctcaggcg agtcaggcgc ctccaaaaag agcttgtaaa gcttgtccgc agaatcgata
    16501 acggatttga ctttcaaaag aagccctgta tgacctattc cagcgaagaa ggatgaaacc
    16561 tgctttcctg ttcccacctc aaacggcttg tcattccaca aagcctctat ctgttctgat
    16621 ccgaggatcc ccttggcaag gagaaaatcc aattcgtcat tgggaacgct ctggcacatg
    16681 atgcggatcg catcaaacga agcgagaatc gccccgagcc cgcgtttcca cccaacatca
    16741 taatcccaca aggcctcccg gctcacgtcc tgcttcttca ctgcctcggc cgccaccttg
    16801 ccggcaatat ccccggcaag aagacccgta tgccaaccat atcccgtaga cggcctgacc
    16861 tggcacgccg cgtcgccaac catcatgaac ccgttggccg aaagctgcgg ttggcaagag
    16921 cgaagcggta tgtacatggg atcgccactc cccctgacca tgcattcagg cccaatggcg
    16981 ggattgtcgt cggcgaattc ctgcaccggc cttaccggac ccgggttccc cggataatcc
    17041 tgaacgccta tgccaatgtt ggcccagttg ttctcttcgc gaacgatcca caagtaacct
    17101 ccatggtacc ccagatagtc gtaatatccg cgatattccg gcggcggaca gtcattggat
    17161 tcccctacta tcctccgcac ctccctgtat cccaagacca catcctcgca gtactgcgga
    17221 tcctgaacga ggatttcggg aggaagcttc ctccgtatga ctgcgtagaa ccccgatgcg
    17281 tccactacga tcctcgcgcg gatctcctct tcgtcgcagg aatcgagatg cctagctctc
    17341 acaccgacca catagccatc ctcaatcaca gggcccacaa cctcagtcct gtacatgaac
    17401 tccacaccgg cgtcggcagc atagccggca agcctctgtc ctaggaggcg acggctgaaa
    17461 aggacgtttg gaacagccgc ctcgaccctg tgtttcttgt taggtgagat caccgtaagc
    17521 acatcgcctg tatcatccag ttccttacct gacggaagcg ggatccccaa ttgttcgaaa
    17581 acatagggcg gggtagagtc gtagctgatc ttccttccta tgtcatcctt tgccctcttg
    17641 tcgatgagca agaccttgca tccgttctcc gcggccgtct ttgcagtcca caagcctgcg
    17701 cttcccccgc cgacaacgat tacatcgtac atcaagcgac cctcctcgca aacggatatt
    17761 cttcggccct atagtccctc tacgagcgtc cgttcaggcc taatctatct cctcaacatc
    17821 aagcgggaac tcggccatca gctcgcagca gttttcgcgt atgagaatcg ggcattcata
    17881 cctgaaccca attttctcct ccgggcacgc atattgcacc gcgcatatga gcatttcccc
    17941 ggcttggtac acatggttgg gatccgtcca gtacttgata ggcccggtat tcaagcctat
    18001 cctccactcg tctcccatca tcccgattcc atgttcaaac cctggaacca cgtaatcggt
    18061 gaacccgcgt tcctccgcca tctccatcac catactggcc agtcccagag gagtaatccc
    18121 ctcccggtag ttgtcgatgc acatctgcac cacgtctatg taattacgcg cgtgccacct
    18181 catcctgtcg gtaacaggag ctataaggta gttataagca taatccccaa gacccagcct
    18241 gtacatggcg tggaggtcta ccatgaggga atcgccgggc tgcagttccc tacgggtcgc
    18301 aggcgtgcat gcccctcccg catatcccgt gcgataccct gaagctatct cgttgccggc
    18361 agtgaaagac caagcccact cgctcccagc cttcctcatt gcacactccg cgatccctgc
    18421 gatctcagtc tcggtcttgc ccttccagcc cccgttcaca agggcctccc tgaccgcctc
    18481 atggccgacg tcggtgtacc gcgaggcctc ccgaaaacgg ttgatggttc cctcatcttt
    18541 gatcagcgac agcctgtcta caatgatatg ggcattgacg aactcgaggc ccggcaacgc
    18601 agcagtaaac atctcatact catactggct gaggttgccc tcaggcgtgt aattggaaag
    18661 gcctgtctcg taagcgagcc gccctttcct tatcttcagt tcatccttta taaagtcgga
    18721 aatctgcgtg atctgatcct gcccgcctaa gggagcataa cccctaacat tgtcctccgg
    18781 aagccatgtg tcatcagcca cccgtgaggc gtcgatgacg aaggtgaaaa gcgttggcag
    18841 cccttcccta ggaatcacga tatagcttcg ccacgggcaa aaggcatcag tagtccacga
    18901 aatggtgcgc aggcgagaac cgaggtaaac atcaaagccc tcattggcca tggcttcctg
    18961 tatgctcttg atcctcccag gataatcgat gaagaacgga tcgttctctg tacaacatgt
    19021 cttctttccc atgatctcgc ctcctcgatt agaattgagt gggaatcgtg gctattacgc
    19081 gattgagcct gtcagccagc gacgacaatt tgagggcttt aaccatgttc cccttcagtt
    19141 tcagcttgcc gctcattagg gcagcctgag ctcccagctc gcccctggac attttggcaa
    19201 acacgtcata gtcgccggaa attgaaaact ccgcttccgg cggctcgccc tcgcccacag
    19261 acacatctcc gatagcccca tccgcgcagc tcacaaagaa atagcgttcc tttccgtcag
    19321 ggcagttgtg atagacggat accaaagacg tagttgcata gttcatcttc tccgggtcga
    19381 gctcggctga aagcctcctg tacgcctcgt cacgccactc agggctgaga tatttgagct
    19441 tgtcttccat agtgaccctc ctcccttgca actcgcgtat tccctcgtct ccccgttctt
    19501 acccgcattc accgggcaca aacactccgt gcaatatcac atcgagcccg ccgttgatga
    19561 ggcgcctaac atctacggcg tccggggtga cgcaggttcc gattatgacc atcttgtata
    19621 tccaaaaaag caggttcgtc acaacctccc ggtctattgg cctgaaacgt ccttcagcca
    19681 ctccgcgctc taacacgtca tccagcattt tgatggactt ttggttgatc tcgaagtacg
    19741 gatcatcctc tccgaataga ggaaagacat tggggtcgcg acttaggatc atttgcatgt
    19801 cgcgattgtt caggagccaa tcgaaggacg actggcataa tgcaacaaac tttctgcaga
    19861 cattctcttc cttcgctacc gcatcggcca cgtatgattg ccactctatc agcgcacggc
    19921 ccacacattt ctcatatagg tcccgcttgc cgtcggaata caggtaaaga gcgctctttg
    19981 tcaatcccag tcgttgagcg atatcatcga gcgtcgcttt cctgtaaccg tattctgcga
    20041 acacctcaaa cgcggccttc ataacttcat cattacgttc ttgttctttc acgtattcag
    20101 cctccatgcc gccatccgtc cacttaatta tttaacgaat cgttcattta ttaatattct
    20161 acacacaatt tttgaccgcg tcaatcccag caatctacta tgtgtccaat ctcaaatgcc
    20221 atattgccca taaagttctc gactcagcgc cttcctctgt cccgtggatc aaaggaacgg
    20281 gaacatgcgg ttcaagatgc ggagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnngacgg atatcgcgtc atgggcccgg ggatgtgtta tcatgtctac gtgggctgtt
    20461 gtggatcagc ccacagcaga ctattatgat gggcgtgcgt aggcgccggg caaggtgatg
    20521 gaagtatgga ttgcaaggca gtagcagatg tcatctgcga ttggctaaag gagaacacgc
    20581 tgcgcgcagg atgcagcgga atagtttttg gactctccgg agggctcgac tcagcagtaa
    20641 tggccggatt gggcatgagg gcaatgccag agggatgcct tggcgtaatc atgccatgtc
    20701 actcagatcc acaggatgtg gaggacgcag acttggtggc ccgctatttc aacctgccta
    20761 cgatcacgta cgatctgggc ccgatctatg acgaacacct cgcaaatctt caacggcttc
    20821 tcgatcctta tgttgctgcc aaggatagaa ggcggctcca tgcagaagat gcagattctc
    20881 tccccgatgg aaccggcgag tcagcttcac gcctggaaag ctcgaaagtc cgaatggctg
    20941 aggcaaacct caagccgcgt ctgaggatga ccgcgctcta ctactacgcc aacgtcctcg
    21001 gctatctggt ggcagggacg accaacagaa gcgagttaac agtaggttat ttcacaaagt
    21061 atggagacgg cgggtccgac ctattgccct tgggcaacct ggtcaagacc gatgtgcgcg
    21121 aactcgcagc atatctgggc gtaccccaga gcattattga aaagccccct actgccggcc
    21181 tgtggagcgg ccagacagac gaaggcgaaa tgggcatctc ctatgccgac ttggatcgct
    21241 acatactcac aggagaagcc tcggacgaag tgcgggaccg agtggaggcg ctaaatcgca
    21301 gcgctcaaca taagttcgtg aggccaccta ttgcccccat ccccagccgc taaatcatga
    21361 ggttggcccc actatccatc tgcgcatttg ccgagcatca acttgcatgg cgcccaggga
    21421 atcccatagg gcttcttatg tgttataatt cttaaaagct cgccccccgc ccgttccaca
    21481 agacccaaat ctcctgcgat ttcaggtagc cctgggccgg gcctgggaaa gaacggagtt
    21541 gcttcaatcg atgcaaaagg ctcttcccgc aacactcgca gtaatgtgta tcggggcact
    21601 gatgatgata ggcctctctc taagcccgcc tcggtcttct gcgcccatca agcccattgc
    21661 caacctagcg gagcgcatta gggatgccgc ggtaatcgcc atatccacgc tcgcagtcct
    21721 tttcatcgga accgtgcttg ccttcgtcat catcgaaagg cagcagagcg gcggcaagag
    21781 aatcttccgc gcgccaaccc atgtccccgg ttgggtccgc atcctatcac tggccttggt
    21841 catgctcgca ttcgtggccg ctacatacac cccgcccaca gagcatgaga agcccacgcc
    21901 gggaccagag gcaaaaggag gcgagaagaa ggcgccggca gagtccgtcg aggaacctga
    21961 gtcggagtat gccccgaggc ctgaacctcc tttagtcgtt cctgccaagg tggccccgtg
    22021 gccctggtgg aaatatgggc tgatctttgc agccatagcc ttcatcgcag tcataggcgt
    22081 cgccgtaatc ggcccacagc gcaccgccgt caccccgaaa cctgttgcgt cagacgcttt
    22141 ggcaatagcg gacatgctgt ccatagagat ggttgacttt tcagacgccg ccatagaggc
    22201 gattgaggaa gaaccagatg cccagatagc ggtcctcatg tgctatctcg cttttctgtc
    22261 tgcattggag atgggcggag ctcccgccga atcccactac actcctgaag aatgcgctct
    22321 caacgccgag aaggccttca atgtgccgcg aagatccctt tcgggtctgc ttgcagcata
    22381 tcagaaagct tttttcagca cccatgatgt gacgagccag gacaaggcag ctgccctggc
    22441 ttcgctgcga agcctcaagt cgcatataga ttccatttct gtcgcacagg cagccagtgg
    22501 ccagaggggg taatcatcca tgtcttccag gttcgatgag gatatcagac acaaagaact
    22561 cgtttctttt gtggagatga ttctgacgct tgcggccttt gccctctttt caatagcgct
    22621 ctacctgctg atgtctccgc acaaacgccc cctggtcccc aagatttccg gggtaggctg
    22681 cctctacatc gcctgcctcc gcgcatggca aggagcaaaa caagggcggg agaatcccat
    22741 gccatggctg gtaacgcagt tcaacttctg ggaatgggtt gactcgtggg cgccatggcg
    22801 caagcggcgc gcatcctccc ctgcagtctg cccccaattc gacgccttcc gccggaaagt
    22861 caggctttct cagggccgaa gccgctacat ggtccgcttc ttagaagaac aagaggcccc
    22921 ggtcgatcta atcgaacatg tcaaaggcgg ctaccatggc aacggagaac gcatgacgcc
    22981 tgatgacata tcttcagtcc ttgatgtgat ggaggagttg ctatgagcat ttcagacatg
    23041 ataatccagg cgtccgcaaa atgtcacgag atcatctcgt cagtatccgc agttatcgtt
    23101 ggcaaagatg acgaacttca gtttgtcctg atgagcatga ttgccgacgg gcaccttctc
    23161 ctggaagacg tgccgggagt cgccaaaact ctaacggccc aatgcttcgc ccaagctctt
    23221 ggcctgaact tcaagagaat tcagttcgtg cccgatcttc tcccgggaga tatcaccgga
    23281 agcacgatat acgatgccag gcgtgaggat ttcaagttca aaccagggcc gatattcgcc
    23341 aatctcatcc tcgcagacga ggtgaaccgg ggcactccaa aaacccagtc ggcccttctc
    23401 gaagcgatgc aagagaggca ggttactgtg gaaggacaaa gctacgtgct tgacccgcct
    23461 tttctcgtaa tagccaccca aaaccccctc gaattcgaag ggacctatcc cctgccggaa
    23521 gcccagatag acagatttat cgccaggctt cggctgggat acccgtcccc cgaagcagag
    23581 cgcgagatcc tctcgcgcag acaggaccgg cgcaccgaca gagcagtcgc gcgccaggcc
    23641 atcgagaaaa gcgagtttct gcagatccaa aacgcagtgg aacatgtata tgtcagtgcc
    23701 gagatccaag attacatagt ctccatcaca acagaaaccc gcaacgaccg cagagtccag
    23761 ctgggtgcat cgcccagagg cagcctggct ctgttcaagc tggcgagagc ccgggcactc
    23821 atgtacgaaa gagactacgt ggtgcccgac gacgtcaaag cagtggcagt ccccgccctc
    23881 gcccaccgca ttcttctccg gcccgagctt tgggctgagc gcattacggg cgaatccata
    23941 gtggaggacg cactcgatcg ggtgccagtc cctgtcgctg attgggcaag agctgttgac
    24001 tagaggcgga ggttgatcgg catgggctcg cggcttactg aaaaaggcta cgtctatata
    24061 gtttccttcg gagctctcgt cctggcaagc atactcctgg accgttggga gttgttgctg
    24121 ctcgcttccg tgttcatgtt cggcctcttc gccgttgcat acgagcagga tgaacctgaa
    24181 tactccatct cagtcaatgt ctcgcatgac agatgctttg aaggcgaaca ggtgttgctg
    24241 gaggtctcga tctcttctgg atccgacatc cctcttcttg aggcccacat cctcctgtgc
    24301 gatgaagaaa accacgtgat ctcttcgaga caactctccc tctctatgag ggcgggagag
    24361 atccgcaatt ttacattccg cgttaacatg ccggcgcggg gcatgttcta cgtgggggac
    24421 acagtctcgc gctcgtacac caggtcaggg ctgaagtcct tcggcgaccg cgtgtggccg
    24481 ggcgaagctt gcctggtcta tccgcgaccg gagcccattc gcgtcgactg gggcctggca
    24541 gccagatccc gccagcacgt tggggaatgt ccttcgcgag tgcccggcga agggttggag
    24601 atatcggaca ttcgcgcagc catgccaggt gaccccgtca aacgagtcaa ctggcgggca
    24661 acgctcaggt tcgggaagct ctacgtcaat gacagcacga gggatcgcaa catcgatgtg
    24721 gtcatagtag tagacggtct cgctgacgtg ggaacatccc cctcaactta cctcgactgc
    24781 gcctcgcgcg cagccgctag catagccatg gggtttttgg aaaaccggga cagggtcgga
    24841 attgtgagat atgcgggcgc agtggactgg gctgtgccgc gtcccggccg caaccagcta
    24901 tacgtgatcc tcgacaggct ggcgcgcctt tacgccgtcc agagttttgt ggcgcccaac
    24961 atgaggcttc ttcccagatc agtcctgccg ccgggatccc ttgttctcgt catcaccccg
    25021 ctcctcgacg acagagccat cgacatgata gtggggctgg cggcccgggg ttgcaatcca
    25081 atggtcctgt atgtctcgcc tataccattc attgaagatc agctcaaaga ctctgccgaa
    25141 gatcagcttg caaaaagatg gtggggcctc gaccacaagg caaaattgaa gaagctcagg
    25201 gcgctcggtc ttcaagttgc agaatgggat ggcaacggct cgctcgatgc ctgcctcagc
    25261 cattacttcg gcggacaagg cttcagatct gcagggcgga gcccttggca aagtgcttcg
    25321 aggggaggat gggcatgaga aagattgact ggcttcgctg gttgcctgcg tttctctgtc
    25381 tcctggtagt catctgcgtg gttggcatca cgcctacagt ctatgtaatg atcccggcag
    25441 ccgccgcgct tgcgctctct ttgtggggga cggtcgcggc aaatttcaag ccgttcggct
    25501 ggagctctgc ttgcgtgcta accgcatgcg ccttgagcct tatggaaaaa aggcctgtag
    25561 cctggtttga gcccgccctc atggggttgg gagtcatctg cttccttggc gcagcccaga
    25621 tcaattctgt atatcagaga aaggatgtct cgcccgatct catggagggt ttcgaggata
    25681 aagctgcgtg cttggaagag aatgatgcgc aagcagaaca accactgatc gactcagctc
    25741 cggccgtccg ctcggcgctc gccatcggcg cacagagagc ggtcgcaatc gtttttgcgg
    25801 ccatggcgtt ttcatacatg ttctttgccc tcgctggcgt cagagcaaag caacttgaag
    25861 aaggattctt catgatgatg ctgggcacaa ctgtaatcgg ggtatcgata tactggctca
    25921 tccgcttagc cgcgcctgac gcgtacaaga aaccccaccg ttgagcgcag gcgcatgcgg
    25981 gtcatgcgcc ttttccgtcc ccgtcccatc cgtagcgtag cagtaaacgc atccgcgcat
    26041 gcacgtatca tagacgccta tgtcgcggct caccacacat ccgcattcgc cacgctggcc
    26101 gggatctttc cgcctgctta cttctacacc caaagccgct cggatcgtgt cagcgtcaat
    26161 acacgctcca tggcctatcc cacatgcaga caagtcatac ggctcagcgc aacttgtgat
    26221 gaccatccca tgacttctgg caatcgcagc aatctcggca aaaagaccca acacatcgct
    26281 tttgagctgc ccaggagcac cgggaacctc cgtctcccac agcgagatct cttccaattc
    26341 ctcaagacgc cgcttcgtct tgcgataaag gtcgagcaga ctcaccgtaa ccctcatggt
    26401 gcttccttca agagccgcag cgatttccga aaacatcttg atgtggtatt caggaggtgt
    26461 gatgttgctc aggattatcg gatcatatct ccaaaaagtt ctggaggggc cgatcacgga
    26521 agcaagctcg acaaatgtgg caatcgcaga acctaggtcc ggcatcttag gctccagcag
    26581 tctaggatag ccattgatcg tatactgaaa cagataacca tatcccatgt tgtcaagctc
    26641 gtgaaggtgc ggcatcatag atctagggtc gcgcgaccag aagacgatcg cgtcgacatc
    26701 atctttggca agggacacca tggacacttt tgtgggacag taagggttgg ggacgagaca
    26761 gaacccggcc cgaatcctgt tcatgaacca ctcgccatag aatgcaggga tatccgtccg
    26821 tctacttgca ctgattatca atcccggtcc ccccaatccc acaatgtcat tgtcatgata
    26881 ccatgcgccg ggcaacccca cccactgcgc accccggccc ctcacacatt tagccatgtc
    26941 agcttcttca accgtacatt cggacagcgc atatgggtat aaaacacaaa ccattgatac
    27001 tcttggaagg aaaactatta tttccgttga acaatatata acctgccatt gttgctcaca
    27061 ttgcctccat atgttatctg cgttttggga aggagttggg catatggtgt cagacggttc
    27121 ctcagctgat atagaaagca agctttccgc agaagagatt cgctatatgc tgcatggatc
    27181 agattcggaa ctcgtcctca attcactgcc tgtgggcgtt gctctcttgc gcatgcccgg
    27241 ctacattctt gagatggcta actcgacttt tgcaaggatg atcggagaag tcaagtcgca
    27301 ttctctggct ccagaaagcc gattcgaaga agtcctgcca aagcagtgga tgaatgtgat
    27361 catacccctg gtcgaccgag ctatagagac caagaatcgt gtcacggtgt gcaaggcccg
    27421 atcgggcgcc gaagaaggag gctccgacgc ttactggctc atcgcctgcc aacctgtacc
    27481 gaacggggcc tccaacacgc accacgcaat catcttgtcc caagatgttt ccgagcaatc
    27541 tgtcctcctc aagagactaa aggccctcag cgccctcgga tcgagcctca gccgcggcta
    27601 tagtctggac gaattcctcg ccgatggagt cggcaaatgc tctgcggcca tctccgcaga
    27661 ctactgtgca gcttttctgc tagatcaaga caccccccgc ctcctgcgcg tcgctgaatc
    27721 caaacccttg tgttggctcc cttcagagat cacatttgat tcgccagagg aatggccatg
    27781 tgggatcgag acgagtgcct cggcctatgt atctttaccc caggcgaacg cacgcgaagc
    27841 aagatggcta ggctccatcg gcgcctatgg gtacttgcgc attccgctca tgacagtagg
    27901 aaggccaata ggggtcatgg cggcggtctt caagaagatc gggtacgtgc cgccccagga
    27961 agacatcgag tttgcggagt tggtagctga aaggtgcgcc atggcgatcg ccgccgatgc
    28021 ggcaatagca gaatacggtc gactcctcgc cgctgagcag gaagcgcatc gcgctgcaac
    28081 agcaaaggcg gcgcagatgg aggcgctcct cgaaagcctc gaatgcggag tggttgcgta
    28141 cgaccgcaac ggctacgtga tactgacgaa ccgtgcgtgc gaagaaatgc tcgacgttgg
    28201 aaagccccag tccaggtatg gggatgatat cgcgttcgga atcactctcg aaaagcttga
    28261 cggagaagcg gtttcattga aggaaatgcc ttactgcaag cttttgagag aagcggccct
    28321 tgccgaccag cactacatga tgcactacac agacggctcc taccgggtca tcagcttcaa
    28381 cggaggcatc gtgcgggacg caaagggcca aatgtcgtct gccataataa tcatgcggga
    28441 cataactcgg atgtatgaaa tggagaaaac ccgagaagaa gtgatcaggc tcatctctca
    28501 cgacctccgc tcgccactga ccctgatact tgcgcgagcg cagatgatgg aacgcgtagc
    28561 cgacaaaccc gacgcagtcc gtaagaacgc catgggaatc gtcttgtccg ccaggtacat
    28621 gaatatcatg atatccgacc tttcggattc tttccgaatt cagtcgggca agatcacgct
    28681 gaaacggcaa gttttcgatc ttgcgaagtt catctctgat tgtgtagagc gctggcttga
    28741 tgcgccagaa acaacgaggc tgaagcttga tattgaaccg aatctcccgc ctgtatacgc
    28801 cgatcccgtt cgcctggaac gaatcctgac gaacctgacc accaatgcct tcaagtattc
    28861 taccggcgat tcagaagtgc ttgtggaagc cgctcgctac caggactccg atgatcaagc
    28921 actcatatcc ataaccgacc acggacaggg cattgccgaa gacgagctgc cccacatatt
    28981 cgaacgctac tggcgcgcct ccgaagctcg agagagatca gaagggatgg gtctcgggct
    29041 gtacatatgt aaagagctcg ttgaggcaca cggaggaaga atctgggtgc acagcgtgca
    29101 gggccaggga agtactttct ttttcactgt gccttttgcc ggataacctg cgccgcccgt
    29161 gcggttccca cccgccgagc tcaccaaacc cctcgccgca atagaggata ttgcgaatta
    29221 tgccgtaatt tcacgccaaa gaatattact caaaacacag caagaactga tacagtcgag
    29281 aatcgccgac caccacaagc aaatagctct gccttgctcg accttggagt ctgtactacc
    29341 tgctttcaat cgattggaag aggggcggat cttgtgcccg cccctcttcg cctgcaaagg
    29401 ccttctatag gtatccgcta gactccaaag ccttcctcgc cgcgtcctta tcttcgtctg
    29461 ctaccatgat tacagggccg gtgcatccca taccggtctc ggcatatatc cccgcgcccc
    29521 acagaacgct cacagcttct tccaactgaa ggatgtccac tcccgcaatg tcggaagtga
    29581 cgactttctt cggcggcggc tctacactct cagcggcagc agccgcgtcg ccctgagccg
    29641 gcgcagacgc cgaagctccc gctccggtgg ggagccctat ctcgggccag cccgccttcc
    29701 gggcggcggc aaattcaacc ctggccacct cgtgaactcc gcccttcacc aggtcagccg
    29761 cgtatctgat cgctccagcc accactggcg cgcccgatgc gcgcgagagg atcatgatga
    29821 tccgcccgta tccctcgccg atcccgggac cataaccgta cccggtcgct tcgtagtccc
    29881 ccccggtgct tgcggccgag aagatcttca tcaagagatt gcctgtgagg ctgtcgcaca
    29941 ccatcacgtc aggagtagcc atcagaagat cgttgccacg catgaccgcg cctccgtcag
    30001 cccgcgccga ttccgcaaac tggatggggt atccggcatc agatagcttt ctcaaagctc
    30061 tctcaacctg ccttgcccca tctatgttga gcacccctac tgtaggctca accaccccgc
    30121 tggctttcgc cgctacgatt ccgtacacag cgttcttcac catgttttcg acgcgctctg
    30181 tccctgatgt gccagtggtt gtggctatga acatctcccg tcccctactt ggggtgacaa
    30241 cccgccccac tgtggagacg cctattggga agttgtagtg catggtgaca catgcatcga
    30301 tctcgccggc atcgagcaat cgctccatct cgcgatgctg ctcttcctcg ctggccgccg
    30361 ggtacagcct gagcccggcc acgccccgca ccgattccgc caactctgaa ggccctatca
    30421 aagccacttc caggtcggaa tattgacgca gcgcacgtag ggccccgtcg ataatctctt
    30481 cgggcccgtg ttcgctgccg agtacagtca ggccgatcct cggcctgcgt ccgcctgcgc
    30541 cctccgccaa accggcaagg tccgctccgc tctcgagagc atcggcaagt ttctcaaaga
    30601 gttcagctat tcttctttta gcatcatcag tcatggcagc cacctcacga ctccactagc
    30661 gtctgcgcag ccccgcgcaa cgcatctgcc atgatcttgc ggagctcgtg ttcggatact
    30721 ccggccatcc ccgctgcagc agcccgatcc cgggaaccgc atttgcccgg gttcttctgg
    30781 ataaggaatg agatcccgtc aaagagattg gtcattctgc ccaggaagag agagcccttt
    30841 ccgattatct gggcgcgttc aattcggccg gcactgatct cgtcacacag atatcccaga
    30901 gacggaactc cagaaggcac atgcccctgc gtaggagcga acccgggcat accatgcttt
    30961 ttcacgaatg ttcccatatc acccctggcg atctcgcccc tctttaccgc aagagctgcg
    31021 atcatcttca gattggatgt gggcacgtcc cctgcacccg caggctcagt tatctccgga
    31081 ttctgcatct cggcagaata tttgtccact tccttcaagc tgagaccgac ccgttccagg
    31141 gggtccacaa ctatggcggt catcaccgcc tgcggagaag agccggaacc gatcttgtgg
    31201 cgtcccaccg cgtcagtgcg gattatgggg cttgtcccgt cattggcaga aacgagaaca
    31261 gcaaatcccc cgagaacgtc ctctagcaga ggaaggccct tcttgacgtg gtccctcccg
    31321 ttcatgccca gcttcgccgt ggccccgcct ccgacgacag ccacgttctt gaatacttgc
    31381 gactgcacga gtcccgcagc gttcaccatg gcatgggccg gcgccgcaca gaatccgcgc
    31441 acatcgcatc cggaagcgtt cacgcagccc gccatctcgc caatggcctt ggcgaagttg
    31501 cctccgccac gctggttcat gtctccgcac gcctcttctg aacattcaat aatatagtcg
    31561 atatctgcag catcgatgcc gcttttcgca accaaatgcc tcagtgcaag cgtccccgaa
    31621 gccttggccg ccaaattctc agccatcacg tgagcagtca ggttcttatc gcgctcatgg
    31681 gctgccttga cgcatcctac caacttatcg ccaatataga gtccttcagc ccggccactg
    31741 gagacaagct cctcgatctg gcttatgggc gccccggccc cgagccgttc ccgatcgccc
    31801 tcgatccaaa cagaatcttt ggagagctcc gccgccacct gcgcagtgaa accctcctcg
    31861 agaacgacca ggtcaaaact gtcaacaatc ttcatgagtg cataaaactc gtcctcaggc
    31921 atgacctctc cccgcgcccc cgcgcggctc gcctttgcca gcgggttctc atgccacggc
    31981 cgaggaatgt tgccaagttc atcaggggtt atgtttccga tgtatgtctg gttcggaggg
    32041 taggccatgc actcgctgta ggaacgcaaa tgcttgggga gctctttcag gtaatcagag
    32101 tccgggttgg cctcccgctc aaccgtctgg gtcgtgccca tgtgcacaag catgcttggc
    32161 gcatgaacaa ggacatagct tacacctcgt acaacagcaa agtccatcca ggcccctcct
    32221 tgcaaaatca agagtagttc gttgttgtcg ggccatgcgg ccccggcgga ccattatgac
    32281 gcgccgaggc cgcacaacgc aaaatggaca gctcaatccc tacttttctt cgaagactgt
    32341 ctgcccttcc acctctgtcg tcatagctgt gagcgcacgc tccaccagac ggcggcgcag
    32401 ttgcttctct tcgtcgtggc ccatcgccgg attgcccagt gggtgaggga tcgccaccgt
    32461 gggtacgatc cggttcgcac ctaccgtaag agaaatcggg acaacggtgc acatatgcac
    32521 aactggaagc cccgcacgct ctatttcttt caccatcgtt gcaccgcaac gtgtacaagt
    32581 ccctcaggtg ctggtgagga tgacgccatt gacgcccgcc tcaaccaatt ccttggcgat
    32641 ggccgccgcg tacttcttcg cgttcgcaac tgaggtacca ttgcccacag tcgtatagaa
    32701 ataccggtgc agcttgccga tgaccccttc cctctccaga tcgcgcatga cgtctacagg
    32761 cagcacccgg ttgggatctg cattagcata gactgggtcg tatcccccat gcgcagtctc
    32821 atgggtctca ctcgtcaagg cttcgacgcc ctcaagatcg tacttgccga acttcgacgc
    32881 cgaagaggac tcaatgtggt cagggttgcc ctttggaacg attcctccgc tggtgaccaa
    32941 ggctattgta gcctttgata ggtcagcaat ggggggattg ggttccacac gatcgaactc
    33001 gggcataggg tactccgtga caaagtcctt gtcggagagc ttcttcacca gcatctcgac
    33061 tgcacgctca gagccgcgct tctcggcaaa gtagttctcc cggatcccgc gggaaagata
    33121 gccatcggtt gcaggatccg tgggctcccc ttttgccaat ttgagtgcaa gctttgccat
    33181 ttgcggcaca gccttgcgca tgccagcggc actgtcgccg gtctcgatga tgtaagcatt
    33241 cttgcggaac atgtccactc cagggttttc aggatacatc ccagtcacaa ccgggattcc
    33301 gaacttctcc gccaccgccg cgcacacagc gccgcaagcg gttccgtatc gtcccgcatt
    33361 gaaggctggc ccagccacga gcagatccgg cttgtatttc tctatgaggg caactacctc
    33421 ttcggtcgcc ttttccatgt tctcgccgaa ataggtgtcg ccgcatataa ctgtcccgac
    33481 gatctcagcc tcatccccaa aggcagcggc caacgccatg ccaggaccca catggcctgg
    33541 cttatcgaag ggagctattc ctgctttgtc ttcgccgcct atctgtccga aaaactggtt
    33601 tagatagtgg actacccgca tcttcttggt cattccgctt ccctcctcac aagagggttt
    33661 agctcaaccc gctgtaagca tcagtcagtt cccgactcgc tgccgccgat tgcggcagca
    33721 ggccctggat cagtacttgc tgtactgctt gcgtatagag ctaacctctt ctttgatggc
    33781 atcgacgtcc aggaccattt ccatcattcc aacctgttcg tcatatacct caggatctat
    33841 ttccgccttt atctcgtctt ccactatgtg ccaaacactc agtcccaact ggactcccgc
    33901 caaaggacct gcgtaggtgg ggtcaccggc ggtcacagtc tctgctgtga gccccgaagc
    33961 ctcagcctcc gctcccccga ggattactac aaggttctct gcgccgtact tctcggccag
    34021 ttcctttact ctcctctggt tttcaatgtc catggcgccg gcagccgttc agacaaagca
    34081 ctcgcttaca gagaagacaa cttcgccgcc ggccgtcttg acgcattctt caatggcgcc
    34141 gcctgggata ccatcgcggt cgcccagagc gacaactttt ttgccgtcga gaatcattgc
    34201 ggcatctcct ttcctagctt cttagttgaa tttagcagtc tcagctcagc aaagccgtga
    34261 tgccctacag caaactcacg cctctctagc tgagagtttg ttgaatccca gttcgttggt
    34321 ggctccagtt ataacctgga gttccgcagt gatcgagccg tccgacgcga gagaaccgtc
    34381 ccaaccccct gcgatgatat tggccgcctc cggatgtccg attaccttct tcataggcgg
    34441 aaggttgacc acttcgttgg cgttgccggc cgttaccact gcgtttgcca gagggttagc
    34501 gtctgcaaga gactgcgacg cgccgtccct gccggcgtac tcgtcggtaa cgaggaccgt
    34561 cttgatcccc ttcttctcca gcttaacgca gttcatgatg aggtcggcat cgggattccc
    34621 gaatccttcc tccgatacta cggcgccttc agccccgagc atctccacta gcttcgaagc
    34681 gaagttggag gagcgctcct tgtcggccaa ggtgacgttc tcgttggtga tcacgacgcc
    34741 gatgaagttg atatcgcgcc cgtggcgtgc taggaggtcg tgtatgaccg gcgagttctg
    34801 atggtggtaa gtagtgttct tgtcacacgc cgacacacag ttccccgata cgatcgcccc
    34861 atccatcacc tcagtcgggt agatgagcgt gggcagaatc ttcttggcgt ctactccata
    34921 tacgtaggtg tcatgcaaca acccctggct ctgcagcatg taaacgtaga ctaccttggg
    34981 cagatcggga taggcattga cctgctccaa caggggcaag cactcgtaga catcgacctc
    35041 gtcgggctca atatcgcgcc ctgcctcgcc cagataggcc gctaccttga ggccggccat
    35101 ccttatggct acctcgtgct cgtgctgcgg caggccggcg atgggttcgc agactacaac
    35161 cacgttgttc agttttgaga acggagtgta gtcggcgccc ggaccagtca tgtcgatgat
    35221 cccctcttgg aagccgacga ctttgccaca ggtcacgaca gcggctcccg aaagaacatg
    35281 agtgcgcccg tcgccgacag tctcaacctt gctgaccatt ccggggaaca cgccgcccgc
    35341 gccttccacc ttgacccgag gctcgatgac gtccttcaca gggatgatcc ttacctcttc
    35401 cccgggcttc gcgagctcca cagtgacgtc ggcgatcctc tcgtcctcgc ggacgatcgc
    35461 ttcgatagcc gctcgatcca cgtagagtac gccgtcctct actttggttg cagggccgaa
    35521 ctgcacgtcc ttaatgcgta ttctgccaag ttccaaccgc atttacttca cctccagagc
    35581 aggactctgc tactgaatct gctccttaat catcgcctct atgttggcag cagtcgcatc
    35641 atccttagtc agctctgcca ccttctcgcc atccttgtag atgcatatgg tcggaaggcc
    35701 gagaaccttc tggcttatag ccagacgcct gttttcaagc acgttgagtt tgcagaattt
    35761 gatctgatct ccgtatttgg cttcaagtgc ctccacatca ggcagaagtg ccatacacgg
    35821 ctcacacttg gtgctccaga aatcgaccaa aactgggcct tttgcctgca agacctcttc
    35881 ctcgaagttt accttgtcta cttggaccat ccttgttcac ctccttaatc tgctaagtgg
    35941 gcccgatagt caaccgtcga aagccgtctg ctcaacctca cttgccggag attaccctgt
    36001 cagctaccag cgtgagttcc tcttcggttg tgggccactg cacccgctcg caaacctgtc
    36061 ctttattgaa gaagagtatc gaaggggcag ccgatatctc gtacctgccg gcaatgcgct
    36121 ggttcttgta tatgtctatt ttgaccaggt tgagcgaacc cgcgcgcgcc tcggcgattc
    36181 tctccacagc gggcataaaa tcgagtgatt cctgggatgt ggggctccag aagcagacca
    36241 tgactgggaa cgctggcgcc agcaccatct cacggaagcc ctcttcctca tggatgtatt
    36301 tttcagcagc caccgcggca attgcgccgt cagcagccgc cgtaatcacc tgcctgagat
    36361 acttgaccct ggcatccccg gcgcagtaaa ccccgtctac cgaagtctcc atctgttcgt
    36421 tggcgattat gtagccccgc ttatccatct ccaccgctcc agacaaaaac tgggtgtttg
    36481 gaactgttcc cacgaagacg aagacgccgt tggtttcaag ctcagtccgt tcattggtct
    36541 ttaggttcct tatgatgacc gactccacta tcccgtcgcc acgtatttcg tcaacgacag
    36601 agttccatac ccatttcatc ttgggattct tgaaagcgcg atcacggatg accgcagtgg
    36661 catcgaggat gccctcgtcg tgaacaacga tcatagtaac ggattcggcg aaccttgtaa
    36721 ggtacatcgc ctcttcaaca gcagcatctc cattcccgat cactacgatg tcaagatcgg
    36781 tgaagaagtc tccatcgcat gttgcgcaat aagacacgcc cttgcctcga agctcccgtt
    36841 cgccaggaag cccaagggtg cggggctcag caccagggca aagtattaca gcactggccg
    36901 agtactgacc tcccccggca gtgcagacta gcttcttttc tcccgaaaag tcgactgatg
    36961 cgacgtcgtc ctttatgatc tctgcgccga attccctggc ctgagctgca aaatgctcca
    37021 tcagctcagg acctttaacg cctcccggaa accctggata gttctccagc cattctgtgg
    37081 taccggcttg cccgccgggc ttcgccttct ccaacaccag tgtggaaagc ttagatcgcg
    37141 atgcatacag ggcagcggac aagcctgcag ggcccccgcc tacgataatc acgtcatatg
    37201 tcttcatatc ttcggccgcc atggttagac gctccttcct tgcctcgaac tagctctact
    37261 gaaaagcctc ggtcttgcaa ccttcaactg ggccattcca cgccgcgtca tgcaggctct
    37321 ctatcgcgct cgccatgaga tcgcagtcga tcaactggta atcatcgtct acgcgcggcg
    37381 gaatatgtgt gaatctgggt gccccaaggt tatgcagaac aaccatggcg ccctcaatta
    37441 tccgcagcga atccaaatcc accattgctg aagcaggata atcagtgatg atgacggatt
    37501 tgtatgggct ctcgttcggc ttcacgcttc cagacagagg atgtgtgaga agaacatggc
    37561 cagcatggac atagtcgcgc acgcagcgga aaacgtcctt gacgcttccc gacacccatt
    37621 cgactgagcc aggccatgtg gcggcaacaa gcgggttatt cgtgatcacg cggtatgtac
    37681 gagaatccat cgtcgccgtc ctcccgaaaa tccaatgcca tattcgatag tcttagcaaa
    37741 cgcagcaaaa agaaaagaca gagaaccgac atggcaaaac aatcctgccg tcgtctctgt
    37801 cataaaacct gagagattta ctccttcggt gcgatatgcg cctctccaga gatgccgtcc
    37861 agctacggtc cttttgcctg aaagttttag gcaaccgcca cggcaatgtg cagtgccttt
    37921 gctccttcgg cgctcacctg caagccgatc atctcatgct tgctgtgagg ctctcccgca
    37981 gcgttccttc ggcgacgcta ttccatcaca gttagtgtaa tgcttcacaa tcgctttgtc
    38041 aacaagtttt tgcataagat atcgaatatc attgtcttct tctatatgtc ctggtccaac
    38101 ttgcggttgt caaacctttt gaacccacgc ctgtccgaca tcgaattgag gtcgccagat
    38161 cggtccctgc agcaaagaca gggtccgcag actgagacag cgccgatatg tcatggaccg
    38221 gttaacaacc ccctcatgat ttcgtgtaca atggcttccc tctcgcgccc aaactctttc
    38281 agtgctctgt tgtcgtcttc agtaatctct acccccgcat cctgcaggat gcgaattgcc
    38341 ttgtcaaact cttccgcccc aacatctgcg tcgccagcca aagcatgata aagcagcaac
    38401 tcgcaacagg acctaacgta gctctgtgga tactccacga cctcgaaatc ccaataagat
    38461 gcaagctcgc agcgggtttg agctgcgagg aagcgatata tctccgcgaa tctctggccg
    38521 gttggggaat aggtcgtccc gttcaatttg aatacaaggc tgatctgagg aggcttagct
    38581 cccaggagag atgggaaccc cttggggctc acagtagcta cgatctccgg gattctgtcg
    38641 ccatcgagat cagcaaagga ttcaggggtc agaaagagat cagttgagcc ttggctgtct
    38701 gtgtatcctg atatgtcaaa tgatctcatg cgatccttct gatctatgta gacgatggaa
    38761 gtcttgtacg cgaaatgccc ttcagacctg cgcaccagca tttccatgca accgtctttg
    38821 ttgatgtctc cgaggagcca cacagacatg gcgtttcctt ctatcttgcc tatgttctcg
    38881 ggctcagccc ccagtccaaa acggattatc gacacagatt ccgtgccggc atcgccaggc
    38941 tcctccaaac ctccgagctc aacacagtag acctttcctc cctgaacgaa catgaactcc
    39001 ttgcgcacag tctcaggcag aagcactgac gcatgacaga tgcccgggaa agaaggaatg
    39061 gcagtcagac aggcctctgc gacatgtatg caagcaagct gctgggctcc gcaggcccac
    39121 ggactcacaa caactgccac tgccgccaca atgagcgcag ctaatagtcc gaaccgctca
    39181 ccaattgtaa atgggagcga caccatacac ccctccaatc gttgaattgt tcataagaga
    39241 gtattcatcg atcgccgctc atagtataga aaccgcagcg gcgttgcaag ttgcacatat
    39301 taaagaagaa tcctccgatt tgacagcagg aatatgagaa aatatatgga atttagaagg
    39361 ctaactgaaa aacgtaggtg atgagcatgg ccataggctt cgagcagggc gcccttcaca
    39421 taaaaagact cagcctcgtc gaagcagtga tgatgcttgt gggcggaaat gtgggggcag
    39481 cgattctgtc cttgccgtat gcagcaagag catctggcta tcttggagct gtgactgtgg
    39541 cggtcataac aactgtcttc tctgcaatct ctcacttgta cattgtagag atcatgctcc
    39601 gcacgaagag cccaggccaa cttgttggtc tggtacgaac ctacatgttc gacagccgac
    39661 atggcaaact ctatctgctt ttggtgtcat ctctgacaat tggattggtc ataccatccc
    39721 tcacagccta tgtgatcggc ggcggcgcaa tcatctcgtc ccttcttgcc ctcccagcgt
    39781 gggcaggcca tctcctgttt ctcctcccag gcgctgccgt ggtatggctt ggcctcgagg
    39841 ctgcaggctt ggcccaagga gtgtccagca tcgccatgat gtcagttctg ctcctattcg
    39901 caggcatctc aatgaggcat ccaagcttcg atcctgcgcg cctggcacgg ttctccgcca
    39961 agccgttggc ggcggcactc cctgtgggta tattcacatc catgtcacag tctttggtcc
    40021 ccgaaatcgt ccgcggctta gcccatgatc ccagatcggc gccgaccgcg atcaagtggg
    40081 gactcgggat caacctcctg tttctgctca tcttccccat ggcaatcttc gggcttcaag
    40141 accccggcga gatttccgag gtcgtaacga tctcgtgggg acaggcgctc ggtcccgcca
    40201 tatacttcgc agtgaacctc ttcgccctgt tggcactcct cacatccttc tggggaggcg
    40261 ccggcgctgc gctgggaaat gtcgtagaga tcttccggtt caaatccgag tgggatatcg
    40321 gggctcgttt ggctgctttt gcaatcacta tcacgccatc ggtcgtccta gcctctgtag
    40381 gcaacttcga tttcgttgat atgattcaga ctgcaggttc agtgggaggc gttatactgg
    40441 cagtgctgcc catcttcatt cttgcaaaag cgcggaaacg ctcagaacgt gaaccagagt
    40501 accaggtggg catcgccttt tccccgatcg tgagagtagc gctcttcgtc ttctatgtag
    40561 gcgtattgat ctacgcggca ttcgccagat agtccgacga ctttgaggta aggtggaatc
    40621 tttgatttgg cctggtggcc atggcgcaac tcgttccgaa cctgaaatta ccgaagtctg
    40681 cgatctcagg aggggtcacg atgatcatca ggtctgctga acccagggac gatgctgttc
    40741 tcctcggcat tgaatcagtg gcagggcagg gagatcagat ccgccttatc ggagagcggg
    40801 acagtttttt cagtagggcc agacagtttg aaaacacgct gctttttgtc ggcgaaaacg
    40861 agaccaccgg caagctgcac ggcgttatgg ccgcctcggt cctgcccgtt cgcgtagggg
    40921 gacgagagcg aataggcgca tacatgttcg acctccgctc caacccggag acgaataagg
    40981 gactgtccag ggcgatgtac ttgatatgga agggccttga ggaccgcctt ctgcagtgtg
    41041 gaacggagtt catatacggg ctgataaagg aggacaactc ccgctcgtac agcattgcta
    41101 cacgcatggg agcaacgccc cgtggtgaga aacgtttcct cacatttccg gtcttccgcc
    41161 gaattagagt ctcccgcgag gtccagacga tttcacatgt ggatgcaatg gaagagcatc
    41221 tcgctgcagc gaagcattat gacggtccat accagctctg gcccatcatg gatgatttca
    41281 gctacatgca aggtctgctg gacaaatacc tagtggccaa gatggaatgt ggatcttctt
    41341 ctctgaagat atgggattct tcactcaact gcgcctaccg cgtctctgcc atgcctagac
    41401 ctttcgagat tctcggcacg gttctcaggg cagccagcca tttggtccct ctgcccaaaa
    41461 tccccatgct cagccaaaag ctgcgtatct ggaacgtgta cgatatcatc acacgtccgg
    41521 gccagcgcag cgaggcgcag gcgctgatcg caaaggcaaa caacttagct ttggaacacg
    41581 gggtagactt tctaatcatc accgtggatg ccgaggagga cgagctccgg tggctcaggc
    41641 gccgctgcct gatgccgctt gattacaaat tgatggttaa ggagtataac cctgttccgg
    41701 agtttttcgg caagacatat ttcgatccaa gatatctcgg gtaaccatcg tccccgcacc
    41761 acatggcgcc tccccgcctt acagacgcat tttaaagact gagcctgggg ttcccgggcc
    41821 gcaacgcggg attcgcccag cgaagagcgg gcaatcgcca gatggttggc tagatgtatg
    41881 tagttgccca tctcatgtgt tcaaccagtt ccgcgacgaa agcatctttc ttctcgatgt
    41941 ggggagaatg accgcattcc tggaaaacaa cttcccggaa gaatccgcca ttcttcgcat
    42001 accggtccaa tacggccctg gtctgctcta tcattggctg aggcggaaaa acctcgttac
    42061 cgggccaacc tggcactaat cccagtctgc ccaagtatcc tatatcaaat ggcgaggcat
    42121 ccgatatgag caggtcgtca gaaccgcgca cccatagtat gggaggtttc acatcgagat
    42181 cggctatgcc atcaaccctg aagtacttcg gtgaaatcgc attgagcacg ccgcaagttc
    42241 ccggggcata tcccggccaa ttgggcgagg gggcgaaatc gcctgggtaa ttgtggtcgc
    42301 caacggctgt ctgaagcaag gcgtccaaga aatcatcttc tcgttcaggg cgaaattccg
    42361 gcctccagaa gcaggtgttc aggatattcc tgggtaaagc cggatcatct cctccatcat
    42421 tcactttcct tttcatcagc tccaagaact gccgattgac gcctccaccg ccggaaccgg
    42481 catagtcagg ccagcacgga atcgcatccg gcccgcgcgt tccggaaaac ccgtaagggg
    42541 atatgggggc cacaagggtc aatgtgatga cctttcccgg gtggtcaagc aagtactgca
    42601 tggcaatgcc tccacctaat gaccagccca caaggtggaa ttctctcaat ccgagcaacc
    42661 aggttaggct gtataggtca tcagaccaat cccggacacc tctcgttgcg tctacgggtt
    42721 tagcttgagt gaggccaaac cccctcatgt caggtgcaac gctcctgtaa tcgtccggaa
    42781 gcgcgaacat cacttcttcc caaaaagtgg ccgatgccgt gtttccatga acgaacacga
    42841 taggctcggc atcgaaatcg cctcttatca ggatatgaaa ctgcgcccgg tctgtcgtgt
    42901 aatatataga ttcaatgcct tcaagctttc ccatagcctg atcgcacctc ccgcgttgtt
    42961 ccgtttctat ccaaactcct gtacaaggag atatatcgat gtccttcatc aacactgctc
    43021 ggtaaaccgc gcaagcctgc attgtccggc ccaaagttga ctgtctttgg gccgcaaggc
    43081 ccggatgccc tcagtattct ccatcataat gcattacacc ttcatataga atcgagcaac
    43141 agcactgttc cggccttggg cgccagtatc gcatcctaag tagctctgaa agggtagttg
    43201 agcgctttca aacggctgtc gtatgggcac cgcccaggaa aagttacccg atgggaaggg
    43261 caaagctcag ttcgaggttg cttagagcgc cgtagataga cacattcctt gaaggcagac
    43321 ccaagtcgcc ctttcatatg agt
//
LOCUS       DAOSYL010000011        41773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_766_length_41773_cov_7.967304, whole genome shotgun sequence.
ACCESSION   DAOSYL010000011 DAOSYL010000000
VERSION     DAOSYL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 41773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41773
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_41773_cov_7.967304"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..895)
                     /locus_tag="PLU15_03400"
     CDS             complement(377..895)
                     /locus_tag="PLU15_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOO29999.1"
                     /translation="MEDALELVAQLMEISARTAPKAVGKDFVVTKVVAGDDLKRLAEQ
                     MELYGQETGKKNYDRDARGVAAAGALLLVGLKDAAPCGLNCGACGYSACADLPDLAEG
                     REFAGPICAWRLVDLGIALGSAAKTASIHNVDNRIMYRIGVVARKMGLIDADVACGVP
                     LSASGKNPYFDR"
     gene            complement(1029..1625)
                     /gene="pyrE"
                     /locus_tag="PLU15_03405"
     CDS             complement(1029..1625)
                     /gene="pyrE"
                     /locus_tag="PLU15_03405"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOO30000.1"
                     /translation="MGNEPGCNDHLKEALEIFTESGALLDGHFLLTSGRHSDRYMQCA
                     QVLKFPQYTEKLARWLAEDFADDQIDLVIGPAMGGIIVAYETGRQLGVPAIFAERDSS
                     GTMALRRGFSITPCQRVLVVEDVTTTGGSVREVMSLVEKSGGIVAGVGVLVDRSNGAI
                     DFGVKQRAVITMEVVSWDAADCPLCRGGSEPVKPGSRK"
     gene            complement(1615..2382)
                     /gene="pyrF"
                     /locus_tag="PLU15_03410"
     CDS             complement(1615..2382)
                     /gene="pyrF"
                     /locus_tag="PLU15_03410"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOO30001.1"
                     /translation="MEPKDALIVALDVDTKKEALAIAHELAGEAGAFKIGMQLFNSEG
                     PDVVRSIQKLGGQVFVDLKFHDIPNTVAAAGRIMARLGCAMFTVHAAGGTHMLKALAE
                     AVKDESAACGADEPLILAVTVLTSIGQEQLDKEMLIHDASVEEVAASWAKLAQECGAG
                     GVVCSPREIVAVRKACGPEIKIVTPGIRPSWSAGLATDDQRRITTPAKAIALGADYIV
                     VGRPITRAENRLEAARRIVMEIAEAKAEKERCLAYGQ"
     gene            complement(2418..3347)
                     /locus_tag="PLU15_03415"
     CDS             complement(2418..3347)
                     /locus_tag="PLU15_03415"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOO30002.1"
                     /translation="MRAEGLAPNLGVDLGGIKLANPVVMASGTFGYGMDYIDLVDPAS
                     VGAVVVKGTTLRPRAGNPPPRIVETPCGMLNAIGLENPGIRVFLDEYLPSLLDRHVTV
                     IANIAGETVDEYAELAAIIDGHGGVAGIEINISCPNVARGGMHFGADPGAAREVVSAV
                     RSVTSLPVIPKLSPNVADIVAIASACQDAGADAISMINTLAGMAIDVENRCPVLGNVF
                     GGLSGPAIKPVALRMIYQVAREVDIPILGGGGIISAKDALEFHMAGATAVSIGTGTFI
                     RPTLAMEVIEGVRDYLISHDLESIDQIIGAALK"
     gene            complement(3329..4213)
                     /locus_tag="PLU15_03420"
     CDS             complement(3329..4213)
                     /locus_tag="PLU15_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOO30003.1"
                     /translation="MERVEEGTVCVETATIIRNDHVGVGLWEMEVAAPRTVAARPRPG
                     QFVHVCTTPPYLAGEHAHPLLRRPFSLYDVDTERESISILYKARGTGSSLMTRFRTGD
                     LVDIMGPLGRGFSVPQRTHTPCALLVGGGVGIAPLVYLARVLVHSGWKVDVLYGANTC
                     GELVALYRLQQLGASCHCTTLDESSGARGVVTDLLPRALEECKPDAVYTCGPEQMMAT
                     VARFAQEFNISGEASLEAHMACGIGACLGCARKLRGSDEGYVKVCVDGPVFPLDSIEF
                     EAEADFGGEEVAGCERKD"
     gene            complement(4213..5523)
                     /locus_tag="PLU15_03425"
     CDS             complement(4213..5523)
                     /locus_tag="PLU15_03425"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOO30004.1"
                     /translation="MRLLLRGGRVVDPANRINTVTCVLVEDGRIAAIGDIPGEESADS
                     GTNVIDCSGKIVCPGFIDLHAHLREPGYEYKEDILTGSRAAAAGGFTTVCAMPNTNPV
                     VDNGAVAGYVARRAREVGIVNVLPIGSITKGQDGEELSEMAELVQAGCVAVSDDGKPV
                     MRSDIMRHALDYARMFDIPVLSHCEDINLSRDGQMHEGFRSTIYGLQGIPAEAEEIMV
                     SRDVMLAKLTGGRLHICHVSTIESLKIIQRAKAEGVRITCEATPHHLTLTDEAVGSYD
                     ADTKVNPPLRSEEHVAALREAVASGLVDCIATDHAPHHVESKDCEYGAAAFGISGLET
                     AVALIMDRLVNTGALQLCTAIALLTTRPAKILGIDKGTLTPGSPADITVIDPDEVRTV
                     DPRRFYSKGKNTPYKGVQLRGWPWMTIVDGRIVFKEGIVTEMKL"
     gene            complement(5507..6448)
                     /locus_tag="PLU15_03430"
     CDS             complement(5507..6448)
                     /locus_tag="PLU15_03430"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOO30005.1"
                     /translation="MNFDERKDLLGLKDLSKEEIELILDTAVPMKDVIRREIKKVPAL
                     RGKGMITVFYENSTRTRTSFELAGKYLSADTSNLAVSTSSVQKGESLKDTIKTIEMMG
                     PDILVMRHSMSGAPHYVARNTTMRVINAGDGTNEHPTQALLDMFSIRERKGTLEGLKV
                     AIVGDILHSRVARSNIYGLSKFGCEVRVVGPSTLMPPEIERLGVKQYFDLKEGISGVD
                     VINVLRIQRERQRTGLFPSLDEYAELYMLRPEHLKFAGDDAIVLHPGPMNRGIEISTE
                     LAESPNTLVNEQVANGVAVRMALLFLMMGGRKDEIAA"
     gene            complement(6630..7178)
                     /gene="pyrR"
                     /locus_tag="PLU15_03435"
     CDS             complement(6630..7178)
                     /gene="pyrR"
                     /locus_tag="PLU15_03435"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOO30006.1"
                     /translation="MQLRDKNVIMDEMDMKRALTRISHEIIEANKGIESVALVGIRRR
                     GCPLAYRLADQIEAIEGAKPPVGLLDITLYRDDLTTLGTQPEVHRTEVYFDVTGLTVV
                     LVDDVIYTGRTARAAMDALMDLGRPRRIQLAVLIDRGHRELPIKPDFVGKNVPTSKEE
                     IISVMVDEIDGENKVVIQELAD"
     gene            7574..9649
                     /locus_tag="PLU15_03440"
     CDS             7574..9649
                     /locus_tag="PLU15_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO30007.1"
                     /translation="MQLLAHTIVKNRHLVLVIALILLIPSVYGVLHARIEYDLMSYLP
                     DDLDSVRGMDILDREFHYADRAIVVIRDDPDWKVLKLKEMIEQVDGVSSVFWISDISD
                     PAIPHDYLGEEIVSQFYKGDITFLLVHLAEGGISLESEKAIADIKALLDDNQRLIGQV
                     VSGIEMKELGMSQTPGMVRAAVILIFLVLLLALPSVALPFIFMATIGAAYAYNMGIAY
                     IFGQRVSYITNSCAAALQLGVTMDYCIFLIHSFQAERKTHDPETAMEKAIASTATAVV
                     ASAITTATGFAALALMSVRLGADMGLFMARGILLSVIATLTVLPSLVLVFQGVIDKFA
                     HRSFLPRFDGIANWAIRRRVPVFALAVVIMAAGYYGYSKVELNYDLEATFPQEIPSIV
                     AMEEFSEAFGSMREAYVVIRDMPGWRLQQITAELREIEGVNSAECLADLVDPAIPLDF
                     VPAEVAKRYVGGGYSNIILNIAGSGLDDTTENLIANVREVLADVDEECYLTGSPVITS
                     DLRRITDRDMVVVNAVSIVAIFVIVMIVFRSPTVAIVLVAAIMSAIWANQAISGILGQ
                     PLFFFSGLAIGAIQLGATVDYAILVASTFREKLGELDPVEGMRSTMIECGPAILNSGL
                     ALFAAIMGVYFATTLLAVKELGLLLARGALISMGVASVLLPPVILVLHPLLVRTSLRW
                     PAGRDLEQN"
     gene            9681..10256
                     /locus_tag="PLU15_03445"
     CDS             9681..10256
                     /locus_tag="PLU15_03445"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO30008.1"
                     /translation="MDTRTLICHGAVRVFAQKGYDATSVDEIAAAIGVAKGTIYYHFK
                     GKRELFVASIKGGLRDLIDEAKAAVDRIGDPIEQLSCIFDTLLERQRKSKDFVYLLFK
                     EAMAPGREWLEEISECWNELASLISHVVDRGKEAGAIQNLETDTITRYILGSMATAAM
                     AGMGDGTQNPRTAEGLKKIMLRGIVTAGCPV"
     gene            complement(10363..11736)
                     /locus_tag="PLU15_03450"
     CDS             complement(10363..11736)
                     /locus_tag="PLU15_03450"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOO30009.1"
                     /translation="MIRVPKTKDGARVGITDTTFRDAHQSLFATRMRTEHMLELAAYL
                     DRVGFYSMEVWGGATFDTCLRYLDEDPWERLRLLRQALVNTPMQMLLRGQNLVGYRPY
                     PDDVVDAFVNKMVELGIDIVRIFDALNDVRNIERAALAVKNAGAHFQGTISYTISPVH
                     TIDGYVKYAQQLKDIGADSICIKDMAGILAPYAAEELVGKLVETVGLPIHVHCHYTSG
                     MASMTYLKAIEAGATVVDCSTSTVALGSSQPPSESMIVALAGSDHDTGLDVRQIRPAA
                     AILREIIENTPEYSDLIAPVQVNTDVLVYQIPGGMISNLRAQLKQMNAENRIEEILAE
                     VPRVREEMGYPPLVTPMSQIVGTQATFNVIQGERYKTISNEVRAYIRGEYGRPPGKID
                     PELKKKAIGDETPTTRRPGDGLEPGMPAARESVAEFGGTEEDAISYALFPNETKKYLA
                     SKAAKGR"
     gene            11927..12868
                     /locus_tag="PLU15_03455"
     CDS             11927..12868
                     /locus_tag="PLU15_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOO30010.1"
                     /translation="MSQLVVVDGDIVDVEEAHVSVLDRANLFGDGLYEVIRCYSGCLF
                     ELATHVSRILEGAKALGILHDWTSESVELAARRLQAETGIMEGEMYIQLSRGPAVRTH
                     YFPKIQSPTMFITLSPVRPIPEGARTVGTSVITCPDMRHGWCHLKTVNLLSNCLAKEK
                     AARHGVWEGLMLRGLDRSALPRTVEEYCNVHGELSGARPGDDVAGWVTEGASANLFVV
                     KDRRIATPPACNILPGVTRRIALELAELAGLDAEKRPVAAKELLTADEVFLTSTIGEI
                     MPVVNIDGHLVGAGEPGPITKRLAEMYTDRVASAACQ"
     gene            complement(12999..14237)
                     /locus_tag="PLU15_03460"
     CDS             complement(12999..14237)
                     /locus_tag="PLU15_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOO30011.1"
                     /translation="MKAIQFEGSVPRYAYSTIFGPLSKRAYYDGLSNIVFRNVSPPSL
                     PNQEWVRVKTKYAGFCGSDKNLIQLHDSPATSPFASFPFTIGHENCGYVVEMGDIAAE
                     RLADKTPLGSRVLVDPLLSCAQRGIDPVCPACARGDYSLCYNFTEGTVSPGFSIGSCR
                     DTGGGWSEEFVAHYTQLIPIPDNVSFEDAVLVDAFCSSLHPVMRNFPRDDSTVLVIGC
                     GVIGLSAIAAIRALGGKARIVALAKYPFQGEAAKECGADEVVYMSRSSDYYTSLAKAL
                     DAKLLDPMLGKKVVLGGADIVYDCVGSSTSIDDALRFTLPHGTMVLVGLASFPKGVDW
                     TPIWLKEVQVRGSFWCSTEKFEGRRMRTYEVAVELLRTERLKLAHFLTHKFALEDYKQ
                     AIEANLNAGHTGLIKSVFEF"
     gene            complement(14234..15430)
                     /locus_tag="PLU15_03465"
     CDS             complement(14234..15430)
                     /locus_tag="PLU15_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOO30012.1"
                     /translation="MSVFQDRIDTFIETYNNLKMSDRYYYLQQVDELDGAHVHIGGRR
                     MVMFSSYSYLGLLKHPKVQERAMEAMDHFGTGTHGVRILAGTTTEHVKCEAKLAEFLG
                     QEDAIAYSSGYVANLSTIQTLLGRNDVVITDKLSHASIIDGCLLCNATFQRFKHNDMD
                     DLKRLLEAGKDKYDGKLVIVDAVYSMDGDVCPLPELVEICRQYGAWLMVDEAHSLGVL
                     GETGHGILEYFHMDPHDVAMLSGALSKTLPAAGGFVAGKHDLIEFLRHNARGFVFSAA
                     IPPASAAAITAALEVIDEEPQLLAAVRRNIDRFVGGLNALGYDTLNTKSCVIPIIIGQ
                     EEPTLELTARLHRDGMFVSPILHPAVPVNTCRLRANVTAAHTDEDIDFALGLLEKHGK
                     DMGLIK"
     gene            complement(15583..16278)
                     /locus_tag="PLU15_03470"
     CDS             complement(15583..16278)
                     /locus_tag="PLU15_03470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOO30013.1"
                     /translation="MSYPERPLSVQRTAPATYTLLVINAIMFGVLEAAGGSQNPFVLL
                     KWGAKFAPAIRAGEAWRLATSVFLHSGLLHLAANSYSLYNLGNVTERLLGTRRFTIIY
                     FLSGIWGSIISTYLSDPMVLGVGASGAIFGLAGCLFYFGVRYPKHFAVIAGSRFILVV
                     LLNLIIGFTNPLIDNYAHIGGLFGGFVAACAFGLPNDPRTETRTLFCWLFIVATLIAI
                     AMIARPLAPGNLM"
     gene            17188..17793
                     /locus_tag="PLU15_03475"
     CDS             17188..17793
                     /locus_tag="PLU15_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO30014.1"
                     /translation="MGDLGWASLSDGELARMAATGSQDAYSELVRRHSEKLLNLAWRM
                     LGSREEAWDAVQETFLSGWQSIGSYRGDAQVYTWLRKILMNVVFGKSRSGGRIQMVSL
                     DEPAKLEGFEIELEVADASLDPQEVVGRMEMIDEIRLAVAELPIIYRSVFVLREAELL
                     SYAEIAKTLGISEGTVKSRLNMARRLLRAKLAEEVAAARNV"
     assembly_gap    17824..17923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17924..18116
                     /locus_tag="PLU15_03480"
     CDS             <17924..18116
                     /locus_tag="PLU15_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30015.1"
                     /translation="RKEGERYFLPTPEGEGLCSVTSDEARKADTSVFGWKRKMLQQAF
                     GLSDVGDAYRRRVAGGQVG"
     gene            18109..18519
                     /locus_tag="PLU15_03485"
     CDS             18109..18519
                     /locus_tag="PLU15_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30016.1"
                     /translation="MDEVEAHLESCALCREKAQSLAALGEGLRCVPNPAWDIEAMVGL
                     VEAEIGSMGRRRAARVWRRSLLPLAAAAAALVILLTSVSSSAFRKQMIKHEEVLIRSH
                     YAVSSSHAGEALVSSRAWPMRGNVSRLQMLEGWR"
     gene            18524..19606
                     /locus_tag="PLU15_03490"
     CDS             18524..19606
                     /locus_tag="PLU15_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HOO30017.1"
                     /translation="MSVRDGRFAWKRAAIIISFLMSASILCQGGVEAINGSPSYGMRP
                     DQLYVLCLTPKAQFTSTMRVWNPSIGNLEVRAAGYGDVVVYSVKLLDCESCLAVSGNM
                     AACYNLPIDKSAMGRVGSVSGGGVVLVPLEESATGRLLAMLMPVGDNLPLLMRNYSIV
                     ESAGYFYCGRESVKLKLVPKVAGNVGRTVWIDEKSGVLLSLEDVNWRGEVIQRVQTLE
                     IDHAPELDAEGLRCARAALAMAANNRGTNKDATAEYASQVLGFKIAIPRWTPAGYELL
                     GVRVLEGVTGRYPLARGVGGVAHLVYYDGLGLISLYERCVPWWAASTLEPRAGNVIEW
                     EQNCIRYLLVGDIDPGLLLRMARSVL"
     gene            19717..20982
                     /locus_tag="PLU15_03495"
     CDS             19717..20982
                     /locus_tag="PLU15_03495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOO30018.1"
                     /translation="MEGFCFVHAADLHLDSPFAAMKVDSPRLAQAFIEASRASLQALI
                     DVCIGHNADFLVLSGDVFDAERRSIRAQLELFEALSRLAERGIHTYMACGNHDALSGW
                     RAILDWPEEVHFFGGDEVESFPVSCGGRVAAVVSGISYAQPAVTDNLALKFRKKLGDP
                     FTIGVLHANVGGDPGYENYAPCTLADLISTGIDYWALGHVHSPKVLSDKDPIVIYPGC
                     TQGRNPRETGPGGCFVVHVGAGGRVEYEFVPVDVIRWHVLDISIDGLACVGDLIEEAK
                     AAIGGLARASDGRSCAVRIRLFGRGPVHHQLVENGEAMDLVSAVVEPAMPGERFDWIE
                     SIQDFTKPEVDREALLSGGSLISDFLALADEAGSDLSGLEGVKEVLVGDLAHSKAAQV
                     IREMDAEGLMRIVERAESIGIDLLLGEEG"
     gene            20986..23892
                     /locus_tag="PLU15_03500"
     CDS             20986..23892
                     /locus_tag="PLU15_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO30019.1"
                     /translation="MRIREVHIDGYGVFRDQHIQDLGDGVALFVGPNEAGKSTLMSFI
                     RGVLFGFDRESQVGYCPPLAGGRHGGRLVVVMDDGEERVVQRYLERGQSRNPADVVGN
                     MSKALYSNVFAFGLSELASLESLGSEEVRARIYSAGAGLGTRTLFEARRVLLSNLDRL
                     YKRQGRVPEINALLREISDLENLIARLADKPAEYNELVRSMEQLVAERERIQEQLDEA
                     KFEHLWFMTLRATRSTWEQFYSARAILDSLEMEDDDGALARREAVMALAPKIRRLKLA
                     QESMVQLVDSIPGLEAVAMERRRSFEERLRELGGAWNEEAVESFDVSPGVVDEIRASR
                     KRISRQDGEVQRLAEAVRARREVADEAESRLARQRERLEEFGELSYATRDDIVARISE
                     IDSAEAILREVSVAEVRAEAAKGNMEAARIRVENATEEVEWRKEEVRRARSFASWLAP
                     AMVAAFSLFAAFGMDRGMGLSIGVAVLGILGAAAIVASILNRAGDAGKKLEAAVEAAR
                     QADELFCCCRDEYVAASGEFARATRAYSSACLGLFGQEDIDGVRLVREREAARSDLAA
                     WDEMARLRRECEQAERDWERAQDEWRKAEEAHRVCLDELERLWEEWRTWMRARGIDPT
                     FGHDAAERTIDAVRDVARARQDMIDARMRLKDARERARRHAADVNEVARSLGLPGTDV
                     DGALVVVDELVAMVEQVEADQEKRARARAVVNELAAQFGSAFPSNDLQRAEEYHLLHT
                     AQEIADLCDDAESRRQALESELKKLAEEQGRKAEKRRLMEHDDEIAIACLELESAKRR
                     LATQVEEWAAYRLCLELIDEACEKYERERQPQVLRDASEALARMTGGRWQSAIARVSG
                     LDSLDVVDDNGRALPHTALSCGTEQQLYLAVRCGLVREYCSHAQSMPVMIDEALVNFD
                     PERAEAAARVLADLADVTQILVFTCHPYMAECFRSTGRVTAQFELDGAEIRPLA"
     gene            24097..25707
                     /locus_tag="PLU15_03505"
     CDS             24097..25707
                     /locus_tag="PLU15_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO30020.1"
                     /translation="MSINGEGMGTVLKRVLTAGLVASLLCLAFAASAAASLLPFDPEW
                     TAAEVGYLVAAGAEVNARDENGWTPLMFAAAWADIETVSVLFEAGADVNASSTSGWTA
                     LMSAMACGNWPQVVGEFRLVGLEDDWSWDWGDEDAGDDWGWDDWWDDDDDWEWDDEED
                     WSWESWYDDDEDWPEEAASPWREPNPLVIRSLVAAGADVNACTEDGWTPLMIAVLCNA
                     DCEIVEDLIAAGAEVNARNEFGQTPLILAVENDTDIEVVAELIGAGARVNAKTKDGMT
                     PLMLAVHNDCPDTIRLLVAAGACANAEGKDGGTPLMHAARFNPNPDVIDILIQAGARV
                     NHRRIDGLTPLMLATFNVNPDVFSLLIAAGADVNARDDEGWTPLMFAAAGSMNHTAIT
                     SLLRADTNVNARTVDGVTALMIAAAETEDVEVVRTLIAAGADVNAKDMCGWTPLMYAA
                     FNVDPKVIAVLVAAGAHVNDRNVEGFTTLMIAADCCENPEVILALLEAGADGKLKSGE
                     GKTAFDYANDNEIIKGTNVYWLLNDARF"
     gene            26078..26410
                     /locus_tag="PLU15_03510"
     CDS             26078..26410
                     /locus_tag="PLU15_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO30021.1"
                     /translation="MQGRDRFHCLSCPVIGSTLAAGADGNLQDEDGWTALMIAAAYCD
                     NPDVILVLLEADADGKLKSKDRMTAFDYARELTCTSSSTRRDSDDSTAAHRLHLAKYR
                     HHDPRGHC"
     gene            complement(26369..27499)
                     /locus_tag="PLU15_03515"
     CDS             complement(26369..27499)
                     /locus_tag="PLU15_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOO30022.1"
                     /translation="MAEALSEFVMSTGFASLGWQHLVMIAISLILMYLAIEKKFEPML
                     LLPISFGMLLANLPATGLTSPPTGDTPGGLLYYLNFGAETGLYPLLVFFGVGAMTDFG
                     PLLANPSTMLLGAAAQSGIFITFFVSLLLGFEPLESACVGVIGAADGPTSIYITSKFA
                     PHMLGPVAVAAYSYMALVPIIMPPIMRGLTTKEERSVIMTQSRPVSKAERILFPIITT
                     VVSALLLPSTAGLIGSLMIGNLFRESGAVERLNKTAQNELTNIVTIFLGLAVGSSATA
                     EKFLSVKTLQILFLGLFAFSAGIACGVFLGKLMYKLSGGKVNPLIGAAGISAVPMAAR
                     VCQQVGLEEDPNNHLLMHAMGANVAGQIASAVAAGVMMAIFG"
     gene            complement(27708..28103)
                     /locus_tag="PLU15_03520"
     CDS             complement(27708..28103)
                     /locus_tag="PLU15_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOO30023.1"
                     /translation="MLEELLSHLEKTSFGIEGFRDHVHRVVGTTVSESEHVHRVDIVT
                     GPPWPGPGGHTHRLLGETDLDDSGHSHQIRGWTRPSSDTSIDHTHQIELQTREIRAHG
                     HKVQGTTASYSPDRWTAMHTRVEDGLRPK"
     gene            28236..29438
                     /locus_tag="PLU15_03525"
     CDS             28236..29438
                     /locus_tag="PLU15_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOO30024.1"
                     /translation="MEYDFDQVLDRRNTNCEKWDYLEEHFGRADVLPMWVADMDFPSP
                     PEVVQAIQERAKHPSYGYTGTSDSFFDSIVGWMDRRHGWAIEREWISTTPGVVPGLAA
                     SVLAFTEPGDNVIVQSPVYPPFFRVVENNGRRLLNNELVRVGDRYEMDWDDLEAKLGE
                     GAKLLLLCSPANPVGRVWSEEELARLSEMCVAHGTVIASDEIHSDIIYSWARHTPFGA
                     LSPEAAENSVTFVAPSKTFNVPGLSTSVAIIPNSDLREGFNRVLSGLGLGTNLFGITA
                     LEAAYTYGDRWLDDAIAYIEENLRFMVEFLGRQVPEIRVVPPEGTYVIWLDCRGLGLD
                     PDALHRFMVEEARVGLSDGTFFGLGGEGYQRINIACPRPILREGLERIASAAVRLRGG
                     VKGTKTGA"
     gene            29486..31075
                     /locus_tag="PLU15_03530"
     CDS             29486..31075
                     /locus_tag="PLU15_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOO30025.1"
                     /translation="MARKRPWFVMVLHSHLPWVMGYGRWPFGEEWIYEIAWDCYIPLI
                     RAFRNLERDGIPANVTLSVTPVLAEQLASESFKSGLVEYLGDRRRRVADDRAEFLARG
                     NTDLARLTIWIEEQIDRALQDFEDMGRDIPGALADLAARGCIELATSAASHGYLPLLK
                     HEKTKRLQIRMGKVGFKEVFGMEPRGFWFPECAYSPGLEELVAGEGYAYTVLDAMAVQ
                     GGKAMSQYGESTRTVPPTGRSVETVYKCCNSELAVFPRHPELCAQVWSRWTGYPGDFA
                     YREFHKQKPRSGMRYYRVTDSAGDLRTKKPYDPTVAAARAREHAAHYVGRIEEAADES
                     RSEVPILTTAFDTELYGHWWHEGPIFLEEVARLLAQSDAVNHATPSRILEEIDWEKVD
                     RIEPLESSWGVNCDHTVWMNECTRPMWEKIWELEDIVNDSWALTPFGPWPGDFWELQS
                     SVDTFLREFLLMMASDWEFLMYTGTAGDYPERRFEGHRKNCAEAYGRLRAALKDHQLP
                     EYLYEPLYDRRDADLESPEGC"
     gene            31154..32551
                     /locus_tag="PLU15_03535"
     CDS             31154..32551
                     /locus_tag="PLU15_03535"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOO30026.1"
                     /translation="MRVLFASSEVNPYMKVGGLADVAGSLPGFLAEAGCDVHLVMPKY
                     ETAKWNGIAFQEWGSVYPPMGYGREEAQVYYSKISRNLTLYAVRNGAYFAREKPYDSP
                     DDMRRFMFFAKACYELARALEVDVVHANDWHTGLLPVFCRVYGCPKDPGTVITIHNLA
                     FQGEGDWDDFLYSSLPWDTFNPGGVEYYGKFNILKAAMMYADRITTVSPTYSREIQTP
                     EFGMGLEKVLMARRDAIRGILNGIDYDEWNPMTDALITSNYDVERLDKRIENKRSLAA
                     RFGLEYRPETPVFGFVGRLFEQKGVDILIEATKRLVEQDVQVVVLGTGAPQYEEALRS
                     LSHAHPGKVGAKLAFSNLLAHEIYAGADFFLMPSKFEPCGLGQLIAMRYGAIPIVRAV
                     GGLRDTVTEPVTGLVFWEYSASELHAAMERALEIYDNPEAFLAMRIRCMTADYSWGRS
                     RDEYVSLYKEIAPGR"
     gene            32756..33271
                     /locus_tag="PLU15_03540"
     CDS             32756..33271
                     /locus_tag="PLU15_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30027.1"
                     /translation="MDDDQTIAKSQKTCKHVTALTSKQTVSYPQLACNERCPETVVRK
                     DQVDKEELKRLVHSISRVLPPEGEIVTEMGVALGAISIEPDRFGWASCSQYVAIVPTL
                     PALVLSCQHLDRPWSWLVWLWVLGSGAGALGGGQGSAFHGRFSGGRGLCSSILSFVMF
                     GLGVLSVYMNF"
     gene            33370..34470
                     /gene="thrC"
                     /locus_tag="PLU15_03545"
     CDS             33370..34470
                     /gene="thrC"
                     /locus_tag="PLU15_03545"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO30028.1"
                     /translation="MRAWRGIIEEYREYLPIERDMPVVTLLEGNTPLLPAGNLAQRLE
                     ADIEIYLKFEGLNPTGSFKDRGMTLAVSKAVQDGAPVVVCASTGNTAASAAAYAARAG
                     LRCIVVIPHAAVASGKLAQARVHGAQIVSIRGNFDVALAIVRELAAETGIVLVNSVNP
                     WRIEGQKTAAFEIVNELGDAPDWLVIPVGNAGNITAYWKGFNECRVVGMASRCPRMAG
                     YQATGAAPIVLGMIVKNPHTLASAIRIGNPASWAGAKTAADDSGGLIDSVSDGEIMEA
                     YSLLAAVEGIFAEPSSCTSVAGVIKMARLGRFEKGQRVVAVLTGHGLKDPEAAMACDA
                     YSLAGRSPEDGSRAVEGVVDADVESVRGLLGV"
     gene            34497..35435
                     /gene="thrB"
                     /locus_tag="PLU15_03550"
     CDS             34497..35435
                     /gene="thrB"
                     /locus_tag="PLU15_03550"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOO30029.1"
                     /translation="MSVKKVIVHAPATSANMGPGYDSMAMALELRDDITFELAGDGES
                     QAITLSEDGSPSPEGLPTDRSNLAIRAFEAVFVEAGERPPSVRVTTVNRIPIARGLGS
                     SAAAIVAGVTAGGVIRGSEIDLQSRLNLAAKIEGHPDNTVACILGGVTVSSLVGSRVM
                     WAKIPLQRELGALVAVPDFGVSTGIARNVLPQSVLHADAVFNVGRAALLVASLCTGAY
                     ENLRYATEDKLHQPYRSLLVPGMLDAIRSACDGGALGAYVSGAGPAILALMDSECDPE
                     PIAQAMAAAFNRSRVKVSDMMALSLSSTGAVWEVEE"
     gene            35432..35998
                     /gene="folK"
                     /locus_tag="PLU15_03555"
     CDS             35432..35998
                     /gene="folK"
                     /locus_tag="PLU15_03555"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOO30030.1"
                     /translation="MSSRSDGTQTGDGSVPVYFGLGSNLGDRLGNLRCAAAMLGQADG
                     LSQFAVSRVYETEPWGFVDQPPFLNCVVRAHTLLEPEVVLAVTRSVEDALGRERSLRW
                     GPRIIDVDILIFGGLVMDTEDLVIPHPRMWERAFVLVPLMDLAPDMITPDGATLSQKV
                     ADLGDAGGVRVFDGDLQGVFDRAENIMM"
     gene            36056..36622
                     /locus_tag="PLU15_03560"
     CDS             36056..36622
                     /locus_tag="PLU15_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30031.1"
                     /translation="MLHLGVGSHRNLRALAVAIPMVLACAAVSFSAGGEREYANREHG
                     FVFRYPDDWERQSGSVGSYPIIAFGELRKTGFAASVNVVVATQPGASAKLYARNAAAR
                     ISQGGDPRLSDFREDGPGVPGAVYGGDSWLTRFSCYSSDVRNRVWFYQLTVVRGSDVI
                     VLTAACLESEKAAYLPVFERIVSSFRLI"
     gene            36643..38361
                     /locus_tag="PLU15_03565"
     CDS             36643..38361
                     /locus_tag="PLU15_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30032.1"
                     /translation="MEYKVVDFEKKKVEIRFGQLAQDLVDFVNLPEFNGTAANGLHKF
                     MSEVMEEPWVDLDDDQDSLENEFVEWLILDYQDEITGVSVVRAYLDSADEWELEDWEA
                     RSLEQWARSVLAVYEVQEILSDDRVVLRDVVTGNTYSVIAPGIGLGVLRWSAMLARLL
                     VVDDLYTLGSSTLVIPRPLLRYVLKIMGHVYRTLRFQGYDGDWESFLKVSTLSLRKLV
                     RVMLMAWGVQESEDAVALYCDGLSGSSSGEFAGVRGACSPSEASASGARKLYRSVVRV
                     SDAKGALDAVTRSGTGIALSSSWVDEAGAVCAELAWVREPHESDPSGVAPYGADDVAF
                     VLLADGAFTVYADNRSALGRCASIIRRSVAQYVIPRQPGKKASSRKPPSTQERARAHA
                     RIDQIAREAIAEHYRSWADSPLPVLQGESPRDAVASGIGQKIVEELLKDIEYHENLKR
                     RAGLPWVDVDGLWHHVLSAQGCDNGLSAKEHSVVGLLDEMMRLDGYTEDQIFATQKIW
                     CDYALSENPNVVHANTWAAAVEYAAAIAQDEPVTQASVARKYQVSVSTLSQRYRQILD
                     SINANG"
     gene            complement(38842..40380)
                     /locus_tag="PLU15_03570"
     CDS             complement(38842..40380)
                     /locus_tag="PLU15_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOO30033.1"
                     /translation="MPKKSRLNLSIVFVLIALLTVFGLYGSAFAAEGTTHITILHVND
                     VHGRMEPFVSDGTDPIGGMTKIAAYIDELRKDPEANLLLLSAGDMVHGTNIVNLFSGM
                     PMIEAMNDMGFAATVLGNHEFNYGQQVLKDLESAANFPFLASNVVYADSGELFVENSF
                     ITEIGGVKIGMFGLSPVETPIVTHPKNVIGLSFADPIETARAVVPKLRAEADIVLCLS
                     HLGYDTDLKLAAAVPGIDIIVGGHSHTEVPEPKLVGGAIVVQAGEWAKHLGVLEIEVV
                     DGKIESFGGRLVAMDASRPNSAPDSGIETIIRKYNAALEDRMAQVVGHTKVALDGERA
                     NVRTRTTNMSNLITDALREASGADIVITNGGGIRASIPAGDITMGNVYTVLPFDNSLV
                     VIELSGDKILAALEHGLSSYPNQAGSFAQVSGLTLRFDPSKPAGSRILEVLVAGEPLD
                     PDRLYTVATNDFMAAGGDGYVWFKEANIIFDSGEMLRDIVAGYLMEHESVEASSEIRI
                     VPVK"
     gene            40628..41269
                     /locus_tag="PLU15_03575"
     CDS             40628..41269
                     /locus_tag="PLU15_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30034.1"
                     /translation="MKKASIMGLALVLVLAASAFASAANYVGGGLGIKAAADASDAKG
                     IIGALSLDFDVMEKLTLSANGYIEFKQKVEEEPPATDAALTMPKFEEPTYFGGVYGKY
                     ALTSFDALKLGAVAGVNFNNGFIVPKEDVESGTDEPAAEPSKVDYGAGIFAEQPLGET
                     GTIYGQAMYWMQEKGVGGLGGINFKLTDKIAVKGQIEYVKKQPLFSIGVGYTF"
     gene            41436..>41773
                     /locus_tag="PLU15_03580"
     CDS             41436..>41773
                     /locus_tag="PLU15_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30035.1"
                     /translation="MKKYAVPIILAFTLVIASGLASASHLSAGYISRDGQLNFTLGGR
                     YDLGRGSSVEGSYTVLPGGYDVAARYKMALYRSSVVAGPVFEGRLIGDHVYTSQALSA
                     GVFAEKIGVTG"
BASE COUNT         8681 a        10356 c        12864 g         9772 t
ORIGIN      
        1 tatcaacctg cgagtgttga aaggctaaat cctgggcgtt ttcggcctaa cggggcgtgc
       61 tccgccccgt cagcggctct acatatcttg ggcaatgaaa accctcaaat agcgcctatg
      121 attgtatggg gtttttgcag gagcaaatgc ttgacgatca ttcgggtcac tcgtgatgtt
      181 gtcgaggccg acaaagtcga tcatcttcac gaggcaaaca tggcatgaaa gcgggtctct
      241 tgccttgtgg acagctgaat gacggggcaa gtggaagcgg caagaagcgt caatggtgtt
      301 gcatcgacgc ttcttgccgc aatacttgat cggtgggttt gcaggctcag gcgatggtct
      361 ttaggacaca ctgcgcctaa cggtcgaagt aagggttttt tccggatgcc gaaaggggca
      421 ctccgcaggc tacgtctgcg tctatgaggc ccatcttgcg ggctacaacc ccgatccggt
      481 acatgatcct gttgtctaca ttatgtattg acgctgtttt cgcagctgat cccagcgcaa
      541 tcccgaggtc aactaggcgc catgcgcaga tcggtccggc aaactctcga ccctcagcga
      601 gatcgggcag gtctgcgcag gcagaatagc cacatgcgcc gcaattgagg ccacaggggg
      661 ctgcatcttt caagcctaca aggagcagcg ccccggctgc agcaacgcca cgggcgtccc
      721 tgtcatagtt tttcttgccg gtctcttggc cgtagagttc catctgctct gccagccttt
      781 tcagatcatc gcctgccact accttggtga ccacgaaatc cttcccgacc gccttgggcg
      841 cggttcgcgc cgaaatctcc atcagttggg ccacaagctc aagcgcatct tccatattcc
      901 tcttcgcgct cccgggccgt gcgggcccgg gctggcgccc tgcctccttt cagatggtat
      961 gctgtagaca tagtacttgg ccaataggca aattcctctt agaaggggac gatggcacgg
     1021 attagcagct acttgcggct tccaggcttc accggctccg atcccccgcg gcagagcgga
     1081 caatcggcgg cgtcccaaga gacgacttcc atggtgatga ccgcgcgctg cttcacgcca
     1141 aagtcgattg cgccgttgct cctgtccacc aaaaccccca cgcccgctac gattccgccg
     1201 gacttttcta ccaggctcat cacttccctc actgagcctc cggtggttgt gacgtcttct
     1261 acgacgagta ctcgttggca tggggtgatg gaaaatcccc gacggagggc catcgtgccc
     1321 gaactgtcgc gctctgcgaa gattgcgggc acacccagtt gtcgtccggt ttcgtaggcg
     1381 acgatgatgc cgcccatagc cgggcctata acgaggtcga tctggtcatc cgcgaaatcc
     1441 tctgccagcc agcgtgccaa cttttcagtg tattgcggga atttgaggac ctgagcgcac
     1501 tgcatgtatc tatcactgtg ccttccggag gtgagcagga aatgaccatc gagaagagct
     1561 ccggattcgg tgaagatctc cagggcctcc ttcagatggt cgttgcagcc aggttcattg
     1621 cccatatgcc aaacacctct ctttttcggc cttagcttcg gcaatctcca tcactattct
     1681 ccttgcagct tcaagtctgt tttcggctcg ggtgatgggg cgtcccacaa ctatgtaatc
     1741 ggcgccgagg gcgatggctt tcgctggagt agttattctg cgttggtcgt ctgtggccag
     1801 acctgcgctc caagatggac gaattccggg ggtgacgatc ttgatttcgg gtccgcacgc
     1861 tttccgcacg gcgactatct cccggggtga acagaccacg ccgcctgcgc cgcattcctg
     1921 cgccagtttg gcccaggatg cagccacttc ctcgacggag gcatcgtgta ttaacatctc
     1981 tttatcgagt tgttcctgac cgatgctggt aagcaccgtc acagcgagga tgagcggctc
     2041 atccgctccg caagcggcac tttcgtcctt cacagcttcg gccaaggctt ttagcatgtg
     2101 ggttccgcct gccgcatgga cagtgaacat ggcgcatccg agcctcgcca tgattctgcc
     2161 tgcagccgca accgtgttgg ggatgtcgtg aaacttgagg tctacgaaca cctgtccgcc
     2221 cagcttttgg atactcctga ctacatcagg gccttcgctg ttgaaaagct gcatcccgat
     2281 tttaaaggcg ccggcttcac cagcgagctc gtgcgctatt gccaaagctt cttttttggt
     2341 gtcgacgtcg agggctacga tgagggcgtc cttaggttcc atatcgcacg ctccttttgc
     2401 ggcggcgtct ttccatctca tttgagagcc gccccgataa tctggtcaat gctttcgaga
     2461 tcgtgggaga tcagataatc cctaactcct tcaatgactt ccatggcaag tgtggggcgg
     2521 atgaaggttc ccgtcccgat cgatactgcc gttgcacccg ccatgtgaaa ctccagggcg
     2581 tccttggccg agatgatacc gcccccgccc agaatgggaa tgtcgacctc ccttgcgacc
     2641 tggtatatca tgcgaagagc tactggcttt atggcgggtc ctgaaagccc gccgaagacg
     2701 ttccccaata cgggacacct gttttccacg tcaatcgcca tgcccgcaag cgtgtttatc
     2761 atcgatatgg cgtcagcgcc cgcatcctgg caggccgagg ctatggcgac gatatcggcg
     2821 acgttgggag agagtttcgg gattaccgga agtgatgtaa cgctacggac cgcgctcact
     2881 acttcccggg ctgcgccggg gtcagcccca aagtgcattc ctccccgcgc cacgttgggg
     2941 catgagatgt ttatctctat cccggctacc ccgccgtgac cgtcgatgat tgcagccagt
     3001 tcggcgtatt cgtcgacggt ttccccagct atgttggcta tcaccgtgac atggcgatcc
     3061 aggagagacg ggagatactc gtcgagaaag acccttatgc cggggttttc aaggccgatg
     3121 gcattcagca tcccgcaggg agtctcgact atccgcggtg gcgggtttcc ggcgcgcggg
     3181 cgcaaggtcg ttcccttgac cacaacagcg cctacgctgg cgggatccac cagatctatg
     3241 tagtccatgc cgtatccgaa ggttcctgac gccattacca cggggttggc aagcttgatc
     3301 cctccgaggt cgactccaag gtttggggct aatccttccg ctcgcatcct gcgacctcct
     3361 ctccgccgaa gtctgcctcg gcttcaaact cgatactgtc caagggaaag acgggtccgt
     3421 ccacacagac tttcacgtag ccttcgtcgc ttccccggag ctttcgcgcg cagccgagac
     3481 atgccccgat gccacaggcc atgtgggcct cgagggatgc ttctccggat atgttgaact
     3541 cttgagcaaa tcgggccacg gtcgccatca tctgctcagg gccacaggtg tatacggcgt
     3601 cgggcttgca ctcttcgagc gcccggggga ggagatctgt caccacccca cgagctcctg
     3661 agctttcatc cagcgtagtg caatggcatg aggcgcccag ttgctgcagc cggtagaggg
     3721 ctactagttc cccgcaagtg tttgcaccgt ataagacatc gactttccag cctgagtgga
     3781 caaggacgcg ggcgagatac acgagaggcg caattccgac ccccccgccg accaggagcg
     3841 cgcacggcgt atgcgtgcgt tgcggcacgg agaaccccct tcctagtggg cccatgatgt
     3901 cgacgaggtc gcctgtcctg aacctcgtca tcaaggatga ccccgttccc cgagccttgt
     3961 aaagaataga gatgctctcg cgttccgtgt ccacatcgta aaggctaaag ggcctccgga
     4021 ggagtgggtg tgcatgttcg cccgcaagat acgggggcgt ggtgcacacg tggacaaact
     4081 gccccgggcg cggccttgcc gccacggtcc gaggtgcggc gacttccatc tcccaaagcc
     4141 ccactcctac atgatcgttc ctgatgattg tcgccgtttc cacgcaaacg gtgccctctt
     4201 cgactcgttc cattaaagct tcatctccgt aacgatacct tctttgaaaa ctatgcgacc
     4261 gtccacaata gtcatccacg gccatcctct gagctgcact cccttgtatg gagtgttttt
     4321 gcccttagag tagaagcggc gcggatccac ggtcctgact tcgtcggggt ctatgacagt
     4381 gatgtctgca ggactgcctg gagtcagagt ccccttgtcg atcccgagga ttttggccgg
     4441 tcgggtagtt aaaagagcga tcgcagtaca aagctgcaat gctcccgtgt tgactagtct
     4501 atccataatt aaggccactg cagtttcaag gcctgatatt ccaaatgccg cagcgccgta
     4561 ctcgcaatcc ttgctctcca catgatgggg cgcgtggtcg gtggcgatgc aatccaccaa
     4621 acctgatgcg acagcttccc gcaaggccgc aacgtgttct tcggagcgca gaggcggatt
     4681 cacctttgtg tcggcatcgt aagagcccac agcctcgtcg gtgagggtga ggtgatgggg
     4741 ggttgcctcg caggtgatgc ggactccctc tgcctttgct ctctgtatga tcttcaggct
     4801 ctcaatagtc gaaacgtggc agatgtggag ccggcctcct gtgagctttg ccagcatcac
     4861 gtctcttgag accattatct cctcagcctc ggccggaatc ccttggagcc cgtagatggt
     4921 ggagcggaac ccttcatgca tctgtccgtc gcgggagagg ttgatatcct cgcaatggga
     4981 gaggacggga atatcgaaca tgcgggcgta gtctagggca tggcgcatga tgtcggacct
     5041 catgaccggt ttaccgtcgt cagaaacggc tacgcatccc gcctggacca gctcagccat
     5101 ttcgctcagt tcctcgccgt cttggccttt tgtgatgctc cctattggga gtacgttgac
     5161 gatgcctact tcacgcgccc ttctggcgac gtatcctgct actgctccgt tgtccaccac
     5221 cgggtttgta ttcggcatgg cacataccgt agtgaatcct cctgctgccg ccgcgcggga
     5281 cccggtcagg atatcctctt tgtattcgta tcctggttcc ctcaaatgtg catggagatc
     5341 gatgaatccg gggcagacga tctttccgga gcagtctatc acgttggtgc cagagtccgc
     5401 gctttcctcg cctggtatgt cgcctatggc ggcgatcctt ccgtcttcca ccaggacaca
     5461 agttacagtg ttaattcggt tagccgggtc tacgaccctg ccgcccctaa gcagcaatct
     5521 catcttttcg acctcccatc atgaggaata ggagtgccat gcggactgca actccgtttg
     5581 cgacttgttc attgacgagg gtgttgggac tttccgcaag ttcagtagat atctcgatgc
     5641 ctctgttcat cggccccgga tggaggacga tggcgtcgtc gcctgcgaac ttgagatgtt
     5701 cggggcggag catgtatagt tcggcatatt cgtcgaggga agggaagagt cctgtacgct
     5761 gtcgctccct ttgaattctg aggacattta tgacgtccac tccgctgata ccctctttga
     5821 ggtcgaaata ctgcttgacc ccgagccgtt cgatctcggg aggcatcaat gtagacgggc
     5881 ctactacgcg cacttcgcag ccgaacttgc tcaggccgta tatgttcgac cgtgccacgc
     5941 ggctgtggag gatgtcgccg acaatcgcga ccttcagccc ttccagcgtg cccttgcgtt
     6001 cgcggatgct gaacatgtca agcagcgcct gagttggatg ctcatttgtg ccgtcgccag
     6061 cgtttatcac ccgcatagtg gtgtttctgg cgacgtaatg gggagctccg ctcatagaat
     6121 ggcgcatgac caggatatca gggcccatca tctcgatggt ttttatggtg tccttgaggc
     6181 tttcgccttt ctgaacgctg ctggttgata cggcaagatt tgatgtgtca gcgctgagat
     6241 actttccggc aagctcgaat gaggttctgg tacgggtgct gttctcataa aagacagtga
     6301 tcatgccctt accgcgaaga gcgggcacct ttttgatttc gcgcctgatc acgtccttca
     6361 tggggacggc agtatcgagt atcagttcga tctcttcctt gctgaggtcc ttgagcccca
     6421 atagatcttt tctttcgtca aagttcaccg tcgcgccccc ttgtgccctg taactgtcct
     6481 tgcgaaggca gaatcgttgc cgcagcttta ggttacaaga aaccttcccc gcagtgcgtt
     6541 gcagggaagg cacaccggca tcatgctgct gcactttccc ttgccagcct cactggacta
     6601 gtttaaaggg actattatat tggaaacact caatcagcaa gttcttgtat gacgactttg
     6661 ttctcgccgt cgatttcatc gaccatgacc gagattatct cctctttcga agtgggcacg
     6721 ttcttgccca cgaagtcagg cttgataggc agttctctgt ggcctctgtc gatcagcacc
     6781 gccagttgga tccggcgagg ccttccaagg tccatgagag catccatggc agctcgcgct
     6841 gtgcggccgg tgtagatcac atcatcgacc aagacgacag tgaggcctgt gacgtcgaag
     6901 tatacttcgg tgcgatgtac ttcgggctgg gtccccaagg tcgtgagatc atctctgtaa
     6961 agggtgatgt cgagtagccc cacagggggc tttgcgcctt cgatggcttc gatctgatcg
     7021 gcgagcctgt aagcgagcgg gcatcccctc cgcctgatgc caacaagggc aacgctctcg
     7081 attcccttat tggcctctat tatctcatgg gagatgcgag tgagcgcgcg cttcatgtcc
     7141 atttcgtcca tgatcacatt cttgtctcga agctgcatgg gccaccctcc ttaattgtgg
     7201 gcataaaaag aacctgcaca agccgtgagg ccaggcagga acagcatgcg tgcagcgatc
     7261 cttgttagcc tcacggggct acccttaaag gttctgctca gtcgcatttg attttaaggc
     7321 aatagggtcc caaagtcaat ggccacacgc acattctctt gcatcccgct caagtgcagt
     7381 acccaaggcg cttggcagcg cattcagtgc taggcacgta cggtgagagg ctgtcaggcc
     7441 gcagcagtgg tcaactatat gagtcctgtt gggctggtgg agtcaattgg gcctgttgac
     7501 aggggaaggg gatagcgcta ccatattact gcactgactg gtcagtgcag taatttgtgg
     7561 atggagatct ggtatgcaac tattggctca cacgatcgtg aaaaacaggc atcttgtctt
     7621 ggttatagcc ttgatcttgt tgattccgtc agtatatggc gtgcttcatg ccagaataga
     7681 atatgatctc atgtcatacc ttccggacga cctcgattcc gtacggggaa tggatattct
     7741 ggatagggag tttcattatg ctgatcgtgc aatagttgtc atcagggatg atcccgattg
     7801 gaaagtcctc aagctgaagg agatgatcga acaggtcgat ggggtctcgt ccgtcttttg
     7861 gatttcggac atttcggacc cagcgatccc ccacgactat cttggagaag aaatcgtctc
     7921 ccagttctat aagggcgaca tcacatttct gttggtgcat ctggcggagg gcggcatatc
     7981 gcttgaatcc gaaaaagcga tagccgacat caaggccctg ttagacgata atcagcgtct
     8041 cataggccag gttgtaagtg gcattgaaat gaaggagctg gggatgagcc aaacgcctgg
     8101 aatggttcgg gcggcggtca tcttgatatt cctcgtgctt ttgcttgctt tgccttcggt
     8161 agcattgccc ttcatattca tggcgactat aggggcggcc tatgcctaca acatgggaat
     8221 tgcgtacatc ttcggacaac gggtgtcata cataaccaac tcatgcgctg ctgcgttgca
     8281 gcttggcgtg acgatggact actgcatttt cctcatccac agtttccagg ctgaacgcaa
     8341 aacgcacgac ccggagacag cgatggagaa ggcgattgcc agcactgcaa cggcggtcgt
     8401 tgcaagtgcc attacaactg ccaccggatt tgcggcgctc gcgctgatga gcgtgaggct
     8461 cggggcggat atggggcttt tcatggcaag gggcatactc ctctccgtaa tcgctacatt
     8521 gacggtactt cccagtctgg tactggtctt tcaaggtgtc atcgacaagt ttgcccaccg
     8581 atcgtttctt cccagatttg acgggatcgc aaattgggcg atccggcgca gagtgcctgt
     8641 ttttgccctg gctgttgtaa tcatggccgc gggctattat gggtactcca aggtagagct
     8701 caattacgac ttggaggcga cctttcccca ggagataccg tcaatcgtcg ctatggagga
     8761 gttcagtgag gcttttggga gcatgcggga ggcctatgta gtcattcgcg atatgcctgg
     8821 ctggcggctg cagcagataa cggctgagct tcgggagata gaaggcgtga attctgctga
     8881 atgcctcgcc gatctggtgg atccggcgat tcctctcgac tttgtgcctg cggaagtggc
     8941 gaagagatat gtgggtggcg ggtacagcaa catcatcctg aacatagcag gctcaggcct
     9001 ggatgataca actgaaaacc tcattgcaaa tgtcagggaa gtattggctg atgtggatga
     9061 agaatgctat cttaccggaa gtcctgtcat aaccagtgat cttcggagga tcaccgaccg
     9121 ggacatggta gtggttaacg cagtctcaat agttgcgatc ttcgtcatcg taatgatcgt
     9181 attccgttct cccaccgtag cgattgtact tgtcgccgcc ataatgagcg cgatatgggc
     9241 caaccaagca atatcaggga tcttaggcca gccgctcttt ttcttctcgg gccttgccat
     9301 aggtgcgatc caactgggtg ccacggtgga ctacgcgatt ctcgtggcca gcacgttccg
     9361 ggagaaactg ggcgagttgg accctgtaga agggatgcgg agcaccatga tagaatgcgg
     9421 gccagcgatt ctcaacagcg gtcttgcgct gttcgctgcg ataatggggg tctactttgc
     9481 aactacactg ctggcagtta aggaattggg gcttcttctg gccagagggg ctctgataag
     9541 catgggggtt gcatctgtac tgcttccccc cgtgattctc gtgctccatc ctcttttggt
     9601 aagaacctca ctaagatggc ctgcaggacg cgacctggag cagaattgac gcaccaagct
     9661 aagaaggaga atacggacaa atggacacaa gaaccctcat atgccacggg gctgtgcgcg
     9721 ttttcgcgca gaaaggctat gatgcgacat ctgttgacga aattgcggcg gcaatcggtg
     9781 tggctaaagg cacgatctac tatcatttca agggcaaaag ggagctgttc gtggcatcga
     9841 tcaaaggtgg cctaagggat ctgatcgatg aggcgaaggc agccgtagat aggatcggcg
     9901 acccgattga acagctcagc tgcatatttg ataccctttt ggagcggcag aggaaatcga
     9961 aggactttgt gtacttgctc ttcaaggagg cgatggctcc tggcagggag tggctagagg
    10021 agatatcgga atgctggaat gagcttgcat ccctcatatc ccacgtagtc gacaggggca
    10081 aagaagccgg ggcaatacag aatctggaga ctgatacgat aaccaggtat atactgggct
    10141 ccatggccac ggcggccatg gccggaatgg gtgatggaac gcagaatccc aggactgcag
    10201 aggggctgaa gaagatcatg ttgaggggaa tagtgacggc aggctgtccg gtgtagcaac
    10261 gggatggacc gcgagatgat gggagaggtg tgccggggcc tgcgaacacc gaaaatgtcc
    10321 gaggccctgt ccgattgagg catattctgt gagcaccctc ttctatcttc ccttggcggc
    10381 ttttgaagcg aggtacttct ttgtttcgtt ggggaacagt gcgtaggaga tggcgtcttc
    10441 ctctgttcct ccgaactccg ccacactttc tctggcagcc ggcattccag gttccaggcc
    10501 atccccgggc cttcgcgtgg tgggcgtctc atctccgata gctttctttt tcagttcggg
    10561 gtctatcttt cctggtggtc tcccgtattc gccgcggatg tacgcacgca cttcattgga
    10621 aattgtcttg tacctttctc cctggatcac gttgaatgtg gcttgggtgc ccacgatctg
    10681 gctcatggga gtgaccaggg gcgggtaacc catctcttct ctcacccgag gaacctccgc
    10741 aaggatctcc tctatcctgt tctccgcgtt catctgcttg agctgggcgc gcaggttaga
    10801 gatcattccg ccgggtatct ggtataccag gacatcggtg ttgacttgca caggagcgat
    10861 gaggtcgctg tactcgggcg tattctcgat tatctctcgc agaatggcgg cagctggcct
    10921 gatttgcctt acatccagtc cggtgtcgtg atccgagcct gcgagagcta ctatcatgga
    10981 ctctgatggc ggctgcgacg aacccaacgc aactgtggag gtggaacagt caactactgt
    11041 tgctcctgct tcaatcgcct tgagatacgt catggatgcc attcctgacg tatagtggca
    11101 atgaacatga atgggcaggc ctactgtctc aaccagtttc cccacgagct cttcagcggc
    11161 gtaaggcgcg aggattcctg ccatgtcctt tatgcatatg gaatctgcgc cgatgtcctt
    11221 gagctgctga gcgtatttga cgtacccatc tatggtgtgc acagggctga tggtgtagga
    11281 tatcgttccc tggaaatggg cgcctgcatt ctttacggca agggccgcgc gttctatatt
    11341 tctcacatca tttaaggcat cgaatatcct tacaatatct attcccagct ccaccatttt
    11401 gttgacgaat gcatcgacca catcgtcggg gtaaggccgg taaccgacga ggttctggcc
    11461 gcgaagcaac atctgcatgg gcgtgttcac aagcgcttgc ctcaaaaggc gcaacctttc
    11521 ccatggatct tcatctagat agcgcagaca cgtgtcgaat gttgcccctc cccaaacttc
    11581 catggagtag aagccgactc gatccaaata ggctgcgagc tcgagcatgt gttcggtgcg
    11641 catccgagtg gcgaaaaggg actgatgggc gtcgcgaaat gttgtgtcgg ttatccccac
    11701 tctagcgccg tcttttgttt tcgggaccct gatcatcttc tccatccccc tttcgggcga
    11761 gattaatcgg cgtcctctga cgcgcttaac acatagtata gcacctcttc tgtcaaatgg
    11821 gcagcaacct agggaggttt tagcagatcc gcttgtggga catcccggtt ggaggatgga
    11881 tgatgttggg tggcgaagat ctctgtagtg cggaggtgaa gtagggatgt cacagctggt
    11941 cgttgtagat ggggatatcg tcgatgttga ggaggcgcac gtgagcgtgc ttgacagggc
    12001 caatctcttc ggggacggac tatacgaagt cattcgatgc tactcgggat gcttgttcga
    12061 gttggcaacc cacgtgtcac gcattcttga aggcgcgaag gcgctcggca tcttgcatga
    12121 ttggacatcg gagtcggtgg aattggcggc caggcggctg caggccgaga cggggattat
    12181 ggagggcgag atgtacatcc agctttcgag gggcccggcg gttcggacgc actacttccc
    12241 caagatacag tcgccaacca tgtttatcac gctttcgcct gtcaggccca tcccggaagg
    12301 agcgcgcaca gtcgggacct ccgtcattac ctgcccggac atgcgacatg gctggtgcca
    12361 tttgaagacg gtgaatctgt tgtctaactg tcttgccaaa gaaaaggccg caaggcatgg
    12421 ggtgtgggaa gggctcatgc tgagggggct ggaccggagc gcgttgcctc ggactgtgga
    12481 ggaatactgc aatgtgcatg gggaactttc cggggcaagg ccgggggatg atgttgcggg
    12541 gtgggttact gaaggcgcca gcgccaatct gttcgttgtg aaggaccggc ggatcgctac
    12601 tccccctgcc tgcaacattt tgcccggggt tacgcgccga atcgcgttgg agcttgcgga
    12661 attggccggg cttgacgcag agaagaggcc cgttgcggca aaggagcttc tcacagcgga
    12721 tgaggttttt ctgaccagca ctatagggga gataatgccc gtggtcaaca tagatggaca
    12781 tttggtgggc gcaggagaac ctggccccat aaccaagagg cttgctgaga tgtacacaga
    12841 ccgcgtggca agtgccgcgt gtcagtgaag ggttgattgt tggccaaaag aatgtagctc
    12901 gccgcgcctt gcgccatgtg ggggcgcgac cgcggcgagc tctttattca ccggctgttt
    12961 gtcctcatca tgccggaaaa cccaagcccg ggcagtttct aaaactcgaa gacggacttg
    13021 ataaggcctg tgtgtccggc attgaggttg gcctcgattg cttgtttgta atcctcgagc
    13081 gcaaatttgt gtgtgagaaa atgggccagt ttcagtctct ctgtgcgcaa cagctctact
    13141 gctacttcat acgtgcgcat ccggcgtcct tcgaatttct ccgtggagca ccagaaggat
    13201 ccgcggacct gtacttcttt taaccatatt ggagtccagt ccacgccctt ggggaaagac
    13261 gctagcccca cgaggaccat ggtaccgtgg gggagggtga accggagggc atcgtctatg
    13321 ctagtggagc tccctacgca gtcgtagact atgtcggcgc cgcccaaaac gactttcttg
    13381 ccgagcatcg ggtcgaggag cttggcgtcg agggcttttg caaggctcgt atagtagtct
    13441 gatgagcgac tcatgtacac tacctcgtcc gccccgcatt cttttgcggc ctctccctga
    13501 aatgggtact tggccaatgc tacgatgcgg gctttgccgc ccaaagcccg gatggcggcg
    13561 atggccgaca gacctattac tccgcagcct atcacgagaa cggtcgagtc gtcgcgcggg
    13621 aaattcctca taaccgggtg cagggaggaa caaaaggcgt ccacaagcac tgcgtcctca
    13681 aaggaaacat tatcaggaat ggggatgagt tgtgtgtaat gggctacgaa ctcctcgctc
    13741 cagcctccgc cggtgtcgcg gcatgagccg attgagaagc cgggggagac cgttccttcg
    13801 gtgaagttat agcacaggga ataatccccg cgtgcgcagg cggggcagac agggtctatc
    13861 ccccgctgtg cgcaggagag caatggatcc acaagaacgc gggagcccaa aggcgtcttg
    13921 tcggccagcc tctctgcggc gatatctccc atctccacta cgtatccgca attctcgtgg
    13981 cctattgtga acgggaacga tgcaaatggc gaagtggccg gcgagtcgtg gagctgaatg
    14041 aggttcttgt cgctcccgca gaacccggcg tacttagtct tcaccctcac ccattcctga
    14101 ttgggcaggg aagggggaga gacgtttcta aacacgatgt tcgagagccc atcgtagtaa
    14161 gcccttttcg acaggggccc gaatattgtg gagtaggcat acctaggaac tgagccttca
    14221 aactgaattg ctttcatttt atgagcccca tatctttccc gtgcttttcg agaaggccga
    14281 gggcgaagtc tatgtcttca tcggtatggg cggcggtgac gtttgccctc agccggcagg
    14341 tgttcacggg caccgccgga tggagtatgg gagatacgaa catgccgtcc ctgtgaagtc
    14401 tcgccgtaag ctctaaggtc ggttcttcct gtccgattat tataggtatg acgcaagact
    14461 tggtgttaag ggtgtcatat cccaaagcat tgaggccgcc gacgaatcgg tcgatgtttc
    14521 ggcggactgc ggccagaagc tgtggctcct cgtcaatcac ttccagcgcc gctgtgattg
    14581 ctgcggctga cgccggcggt attgctgcgc tgaagacgaa cccacgggcg ttgtggcgga
    14641 ggaactcaat gaggtcgtgc ttgcctgcaa cgaagccgcc tgcggccggc agggtcttcg
    14701 agagagctcc gctcaacatc gccacgtcgt gggggtccat gtggaaatac tcgaggatgc
    14761 cgtggccggt ttcgcctaga acgcccaatg aatgggcctc atcgaccatg agccatgcgc
    14821 cgtactgccg acagatctcg acgagctcgg gaagtgggca gacgtctcca tccatgctgt
    14881 agactgcatc cactatcacg agtttgccgt catacttgtc ctttccggcc tcaagaagtc
    14941 gcttcaggtc atccatgtcg ttgtgcttga aacgttggaa tgtggcgttg cacagtagac
    15001 agccatcgat gatgctagcg tggctcagct tgtctgtaat gaccacgtcg tttcttccca
    15061 gcagagtctg tatcgtgctg aggtttgcga catatccaga gctgtaggct attgcatcct
    15121 cttgacctaa aaactcggcc agttttgctt cgcacttcac gtgttcggtg gtggttccgg
    15181 ctagaattct gacgccatgt gttcctgtcc cgaaatggtc catggcttcc attgcccgct
    15241 cttgcacctt ggggtgtttc agaaggccca ggtagctgta ggatgagaac atcaccattc
    15301 gccttccgcc tatgtgcacg tgcgcgccgt ccaattcgtc tacttgttga agatagtaat
    15361 acctatcact cattttcaga ttgttgtagg tttcgatgaa ggtgtctatg cggtcttgaa
    15421 atacgctcac gcttaaccct ccctgcctgt gttcacaaaa atgtgcacac acacatatta
    15481 ctaccccgga tgtgtggtgt caacgcagga tggactcggg ttggctgaga ttgttacagt
    15541 taaaatgggt gcattgacgc tgactgtgtg aggttggcag gactacatga ggttccccgg
    15601 ggcgaggggg cgcgctatca tggctattgc aatcagtgtg gccactatga ataaccagca
    15661 gaacagggtt cgcgtttcag ttcttggatc gttgggaagc ccgaacgcgc aggcggctac
    15721 gaatcctcca aataatcctc cgatatgggc gtagttgtcg atgagggggt ttgtgaagcc
    15781 tatgatcagg ttgaggagga ctacaaggat gaaccttgat cctgctataa cagcaaagtg
    15841 tttggggtac ctcaccccaa aatagaagag gcatccggca agcccgaata tggcgcctga
    15901 ggcccctaca cccaacacca ttgggtctga aaggtatgtg ctgatgatcg atccccatat
    15961 gcccgagaga aagtagatga ttgtgaatcg tctcgtcccc aagagtcgtt ctgttacgtt
    16021 ccctaggttg tagagggaat acgagttcgc ggcgaggtga agcaatccgg aatgaagaaa
    16081 gacggaagtc gccagtcgcc aggcttcgcc cgcgcggatc gccggcgcga atttggcgcc
    16141 ccatttcagg agcacgaacg ggttttgtga tcctccggct gcttcgagca cgccaaacat
    16201 tatcgcgttt atcacgagaa gcgtgtaggt ggctggcgcg gttcgctgga ctgacagcgg
    16261 ccgttccggg tagctcattc gtatccctcc caacggaccc taatgcaaat agtatacgat
    16321 atgcgccatg tgcatcaaga taccgcatgc gaacgttcgg cgcatttccg gcaatctgtc
    16381 cgtaatatgg ttgtctccgg catcgggaag gagcaacaaa actgccgtta accggagtgg
    16441 gcggcccgct caatcgataa atggggcgaa tttatgttga tatagataaa aaatcgtact
    16501 tgtttcgaac ttttttgctc caattgcatc tatagtagta gagccggggc aatgcccaga
    16561 cgaattccat atagctgagc cacccagaat tgtacttacg ctttgaaaga agtaaggtta
    16621 ccaccaatag acatacatgc atcaggagaa ggcagcaaga tcagtttgcg taacatacct
    16681 cctaggaaac gcagcaggtt tggatactga aaaggcgcgg ggatgcaatt atctggcggg
    16741 ggtaggagca tatccttgca ggcaagttac gggtgtaagg gatgtagttg gttcagcatg
    16801 gcacgtctgg cagttagcct cggctattct tttcccgaga tcaaaaggtg gatgtcgcca
    16861 cgctcgactc cagtatcgca taattgatac accaaatagt agggagcccg caggcttgca
    16921 gggtactgcg gccttatggc gaatccaaaa tggtgtagtg ctgggcgatg caatccgcca
    16981 atttgcatat ttgcatccag atggcggggg ccaggttggg gcttgtgttt cggcaggtag
    17041 ttgccggtat tggcaacgcc cccgttgctc aattatgccc cctgtcgcct gagaaagccg
    17101 gcggcttaca gggcttctga aaacggggcc caagaggagt cgcggctgaa gtcgctttcg
    17161 gcctgttcat ctcgggaggt gaacactgtg ggcgatcttg ggtgggcttc tttgtccgac
    17221 ggagagctgg cgcgtatggc ggcaacgggt tcgcaggacg cctactctga actcgtcaga
    17281 aggcactcgg agaagctctt gaaccttgct tggaggatgt taggctctcg ggaggaagcg
    17341 tgggacgctg ttcaggagac ttttctgtct ggctggcaaa gcataggaag ttacagggga
    17401 gatgcccaag tctatacatg gctacgcaag atactcatga acgtggtttt tggcaagtcc
    17461 agaagtggcg gtcgcattca gatggtatcg ctggatgaac ctgcaaagct cgaaggcttc
    17521 gagattgagc tggaagtcgc tgacgctagc ttagatcctc aagaggttgt tggacgtatg
    17581 gaaatgatcg acgagatccg ccttgccgtg gctgagctgc cgataatcta taggtcggtt
    17641 tttgttttga gagaggctga gctgttgtct tatgctgaga tagcgaagac tctgggaatt
    17701 tccgaaggta cggtgaaatc caggctgaac atggccagac gactgttgcg ggcaaagcta
    17761 gctgaggaag tggccgctgc gagaaatgtt tgaagctgat gaagcgctct cttggccaag
    17821 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgaaag gagggagaac
    17941 gctacttcct tcccacacct gaaggagagg gtctctgtag cgtgacatct gatgaagcaa
    18001 gaaaagccga tacgtctgtg ttcggatgga aacgaaagat gttgcaacaa gcatttggat
    18061 tgtctgacgt tggggatgca tatcgacggc gagttgcagg gggtcaggtt ggatgaggtg
    18121 gaagctcatc ttgaatcgtg cgcgttatgt cgcgagaaag cccagtcttt ggccgcgctc
    18181 ggagaagggt tgaggtgcgt acccaaccct gcctgggata tagaggccat ggttggtcta
    18241 gtggaggcag agatcgggag catgggcagg aggagggcag cgcgagtttg gcggaggtct
    18301 ttgctgcctc tggctgctgc tgcggcggcg ttggtgattc ttttgacctc cgtttcgtcc
    18361 agcgcgttca gaaaacagat gatcaaacat gaagaagtgc tcatcagatc ccactacgca
    18421 gtctcctcga gccatgcggg agaggctctt gtctcaagtc gtgcctggcc catgcgggga
    18481 aacgtatccc gtctccagat gctcgagggg tggcggtaat gggatgagtg tgcgcgatgg
    18541 cagatttgcc tggaaaagag cggcgattat cataagtttt ttgatgtcgg caagtattct
    18601 gtgccaaggg ggagtagagg caatcaacgg gtcgccgtca tatggcatga gacctgatca
    18661 actttacgtc ctctgtctga cgcctaaggc gcagtttacg tccacaatga gagtatggaa
    18721 cccttccatc ggaaacttgg aggttagagc tgccggctat ggcgacgtgg ttgtgtactc
    18781 tgtgaagctg ctagattgcg aatcatgctt ggctgtgagc ggcaacatgg cggcatgcta
    18841 caatcttccc attgataaat cagctatggg cagggtgggc tctgtgtctg gcggaggcgt
    18901 cgtgctagtg cccttggaag agagcgccac gggacggctt ttggcaatgc ttatgccggt
    18961 gggtgataac ctgccgctgt tgatgcgcaa ttactccatc gtggaatctg caggctactt
    19021 ctactgcgga cgggagagtg tgaagctcaa gctcgtgccg aaggtagcgg ggaatgtcgg
    19081 gaggactgtg tggatcgatg aaaaatccgg ggtcttgctc agccttgagg atgtgaactg
    19141 gcgcggagaa gtgatacagc gcgttcagac tctggagata gatcacgccc ctgagcttga
    19201 tgcggagggg cttagatgcg ccagggcggc gctggctatg gccgcgaaca ataggggcac
    19261 gaataaggat gctacggcag aatacgcctc gcaggttctc ggcttcaaga ttgcaattcc
    19321 cagatggacg ccggcaggct acgagctttt gggcgtgagg gttcttgaag gtgtcacagg
    19381 tcgatatccg cttgcccggg gtgttggggg cgttgcccat cttgtgtact acgacggtct
    19441 tgggctgata tcgctttacg aaagatgcgt tccgtggtgg gccgcttcta cgttggagcc
    19501 tagggcaggg aatgtgatcg aatgggaaca gaactgcatc aggtatctgc ttgtaggcga
    19561 catagatccg ggactcctcc tcagaatggc ccggagcgtg ctataaacgg ccccggcggc
    19621 cgatccagac aatcgatgat tgctggaggg taaacccctc tgagcagaga ataccctttg
    19681 gggtgtgaat tcctgctcgg agggggttgc tctattttgg aaggattctg ctttgtccac
    19741 gcggcagatc ttcatctcga ttcgccattt gcggcgatga aggtggattc tccccgcttg
    19801 gcccaggcat ttatagaggc gtcaagggcc tcgcttcagg ctttgatcga cgtatgtatc
    19861 gggcataacg ccgatttttt ggttttatcg ggagatgtgt tcgatgccga gcgacggagc
    19921 atcagggccc agctggagct cttcgaggcc ctctcaaggc ttgccgagag agggatacac
    19981 acatacatgg cgtgcggcaa ccacgatgcc ttgtcgggat ggcgggcgat cctggattgg
    20041 ccggaggagg tccacttctt cggaggagac gaggtggagt cgtttcctgt gtcgtgtggc
    20101 ggccgagtgg cagcggtggt ttccgggatc agctatgcgc agcccgctgt aaccgacaat
    20161 ctggccctca agttcaggaa gaagctagga gatccgttca caattggggt tctccatgcg
    20221 aatgtgggag gcgacccggg gtacgaaaac tatgctccgt gcaccctggc ggacttgatc
    20281 tctacaggca ttgactattg ggccctgggc catgtgcatt ctccaaaggt actgtcggat
    20341 aaggatccga ttgtgattta ccccggctgt acccagggga ggaatccacg ggagactggg
    20401 cctggcggat gtttcgtggt gcatgtgggc gctggaggac gggtggaata cgaatttgtc
    20461 cctgtggatg tgatacgctg gcatgttctg gacatctcta tagacggctt ggcctgtgta
    20521 ggcgatttga ttgaggaggc aaaggccgct atcggcgggc ttgcgcgggc ttccgacggc
    20581 aggtcttgtg cggttcgcat ccgtctgttc gggcgcggtc cggtccacca tcaacttgtt
    20641 gaaaacggcg aggctatgga tcttgtgtcg gcggtcgtgg agcccgcgat gcccggggaa
    20701 cggtttgatt ggatcgaatc gatccaggat ttcaccaagc ctgaggtgga cagggaggcg
    20761 cttctttcgg ggggaagctt gatttccgac tttttagctc tggcagatga ggctggctcg
    20821 gatctttccg ggcttgaagg ggtaaaagaa gttttggtgg gggatctggc tcactccaag
    20881 gctgcacagg tcatcagaga gatggatgct gaggggctta tgcgaattgt ggagagggcc
    20941 gagtcgattg ggatcgacct tcttttgggg gaggaggggt aggggttgcg catacgtgaa
    21001 gtccacatag acggttacgg ggtctttcgt gatcagcaca tccaagatct cggcgatggg
    21061 gtcgctctat tcgtcgggcc caacgaggcg ggcaagtcca ccttgatgtc atttatccgc
    21121 ggggtgttat ttgggttcga tcgggagtct caagtaggct attgtccgcc tcttgcagga
    21181 ggccgccacg gcggaaggct tgtggtggtg atggacgatg gcgaggagag ggttgtgcag
    21241 cgttatcttg agcgtgggca gtcccgcaat cccgccgatg ttgtgggcaa catgtcgaag
    21301 gctctctact ccaacgtgtt cgcgttcggc ctgtctgagc tggcttcgtt ggaatccctc
    21361 ggatctgaag aggtgcgggc gaggatatac agcgctgggg cgggcctcgg cacgcgcacc
    21421 cttttcgagg cgcgcagggt tctcctctcc aacctcgatc ggctctacaa aaggcaagga
    21481 cgggtgccgg agataaatgc gttgctcagg gagatttcgg acttggagaa cctcattgca
    21541 cggcttgcag acaagccggc ggaatacaac gagcttgttc gttcaatgga gcagttggtg
    21601 gcggagaggg agaggatcca ggagcagttg gatgaagcca aattcgaaca tctgtggttc
    21661 atgaccctca gggctacccg gtcgacgtgg gaacagttct attcggcgag ggccatatta
    21721 gacagcctcg agatggaaga cgatgatggc gcattggcca ggagggaggc ggttatggcg
    21781 ctggccccga agatacgccg cctcaagctg gcacaggaga gcatggttca gctggtagac
    21841 tccattcctg ggcttgaagc tgtggcgatg gagcggcggc gatcgttcga agaacgcttg
    21901 agggagctgg gcggggcgtg gaatgaagag gctgtggagt ctttcgatgt gtctcctggc
    21961 gtggttgatg agattcgggc ttcacgtaag cggatttcga ggcaggatgg agaagtgcag
    22021 cgccttgcgg aggcggttcg ggctaggcgc gaggttgcgg atgaggccga atcgaggctg
    22081 gccagacaga gggagcggct tgaagaattc ggggagttga gttacgcgac cagagacgac
    22141 attgtggcgc ggatcagcga gattgacagc gcagaagcga tcttgaggga ggtcagcgtt
    22201 gccgaggtga gggctgaggc tgccaaaggg aacatggaag ccgcgaggat ccgcgtggaa
    22261 aacgcgacgg aggaggtgga gtggcgtaag gaagaagttc gccgggcgcg aagcttcgcc
    22321 tcctggttgg cccctgccat ggttgcagct ttttcccttt ttgccgcttt cggaatggac
    22381 aggggaatgg gtctgtccat tggagtcgcg gttttgggaa ttctcggtgc ggctgcgatt
    22441 gtcgcgtcga ttctgaatag ggcgggggac gccggtaaga agttggaggc ggctgtggaa
    22501 gcggcgaggc aagccgatga gcttttctgt tgctgccggg atgagtatgt tgctgcatcg
    22561 ggcgagttcg cgagggctac tcgcgcatat tcctctgcgt gtttggggct tttcggccag
    22621 gaggatatag acggggtcag gctggtgcgg gagagggagg cggccaggag cgacttggca
    22681 gcatgggatg aaatggctcg gttgcggcgc gagtgcgaac aggcggagag ggattgggag
    22741 cgggcgcagg acgaatggag gaaggctgaa gaggcgcaca gggtgtgcct ggatgaactg
    22801 gagcgtctgt gggaggaatg gcgaacctgg atgagggcgc gggggattga tcccaccttt
    22861 ggccatgatg cggcagagag gacgatagat gctgttcgcg atgttgcgcg ggcgcggcaa
    22921 gacatgatcg atgccaggat gaggctgaag gatgcgaggg aacgcgccag gaggcatgcg
    22981 gctgatgtga acgaggtcgc gagatcgctg gggctgccgg ggacggatgt ggatggtgca
    23041 ttggttgttg tagacgagct cgttgcaatg gtggaacagg tcgaggcgga tcaggagaag
    23101 agggcgcggg cgcgagcggt ggtgaacgag cttgctgcgc aattcggttc ggcctttccc
    23161 tccaatgatc tccagcgtgc tgaggaatat catctgttgc atacggccca agagatagcc
    23221 gacttgtgcg atgatgccga aagccgtcgt caggctttgg agtcggaact gaagaagctt
    23281 gcggaagagc aggggcgaaa ggctgaaaag aggcggctca tggagcatga tgacgagatc
    23341 gccatcgcct gtttagagct ggagtcggcc aagagaaggc ttgcgacgca ggtagaggaa
    23401 tgggcggctt ataggctatg ccttgaattg atcgatgagg cgtgtgaaaa gtatgagcga
    23461 gaaaggcagc cacaggtgct tcgcgacgcg tcggaagctc tagcccggat gaccggggga
    23521 cggtggcagt ctgccatagc gcgggtatcg ggcctcgatt ccctcgatgt ggtggatgac
    23581 aatggcaggg cattgcccca tactgcactt agctgcggaa ctgagcagca gctctacttg
    23641 gcggtcagat gcggcctggt tagggaatac tgctcccatg ctcaatcgat gccagtgatg
    23701 atcgatgagg cgcttgtcaa tttcgatccc gagcgagctg aggcggctgc acgtgttctg
    23761 gcggatcttg cagatgtcac tcaaattctg gtcttcacgt gtcatccgta catggcagaa
    23821 tgcttcagga gcacgggcag ggtgacggcc caattcgagc tggatggtgc cgagatcagg
    23881 ccgcttgcct gaggtttcgg tctgcttagg ggactgctat ttgtcccttg gtccgacccc
    23941 aacatgcctt gtcagactgg agctacattc acatcacatg atcggtacaa agaccaaacc
    24001 ttgccacagg tgtgcatata tgtggcaatc actatcactg agcagatggg tttctcctgg
    24061 gtgaagccgc ttctttcgac gatgccatag gaggcaatga gtattaatgg ggaagggatg
    24121 gggacagtgt tgaaacgggt attgaccgct ggcttggtcg caagcctctt gtgcttggcg
    24181 tttgctgctt cggctgctgc ttctctcttg ccgtttgatc ctgaatggac tgcggctgag
    24241 gtgggctact tagtggcggc gggcgcagaa gttaatgcaa gggatgagaa tggttggacg
    24301 cccttgatgt ttgcagcagc atgggcggac attgagaccg tttccgtgtt gttcgaggcg
    24361 ggtgcagatg taaatgccag ttcaactagt ggttggacgg cactgatgtc tgcaatggca
    24421 tgtggcaact ggcctcaagt ggttggggaa ttcaggttgg tgggcctaga agacgattgg
    24481 tcttgggact ggggtgacga ggatgccggt gatgactggg gctgggatga ttggtgggac
    24541 gatgacgacg attgggaatg ggacgacgaa gaggactggt cttgggagag ctggtatgac
    24601 gatgatgaag actggccaga agaggcagct tcaccatggc gcgaaccgaa tcccctggtt
    24661 attaggtcgc tggtagctgc gggtgctgac gtgaacgcgt gcacagagga tggctggacg
    24721 ccgctgatga tcgcagtatt gtgcaatgct gattgtgaaa tcgttgagga cctgattgcg
    24781 gcgggcgccg aagtgaatgc aagaaacgaa tttggccaga cgccattgat acttgcagta
    24841 gagaatgata cggacattga agtcgttgct gagctcatcg gcgcaggcgc tagggttaac
    24901 gcgaagacga aagatggcat gacgcctctg atgcttgcgg tacataatga ctgtccggat
    24961 actattaggc tgcttgtggc tgctggtgct tgtgcaaatg cagaaggcaa agatggtggg
    25021 accccgttga tgcatgcggc gagatttaac ccaaatcctg acgtcattga tatacttatc
    25081 caagcaggtg ctcgcgtgaa tcacagaagg atagatggac taactccgct tatgcttgcg
    25141 actttcaacg tgaaccctga cgtattcagt ctgctgatag cggccggtgc tgatgtgaat
    25201 gcaagggatg atgagggatg gactccgcta atgtttgcag cagcaggcag catgaatcat
    25261 accgcgatta cttcgcttct gagagcagac accaacgtga atgcacgaac ggtggacggt
    25321 gttacggctc tgatgattgc agctgccgaa acagaggatg tagaagtcgt caggacattg
    25381 atagccgcag gcgccgacgt gaatgcaaag gacatgtgtg ggtggacacc gctaatgtac
    25441 gcggccttca acgtggatcc gaaggtgata gcggtgctcg ttgcggcagg cgctcacgta
    25501 aatgacagga atgtggaggg cttcacgacg ctaatgatag cagcagattg ctgcgagaac
    25561 cctgaagtca tccttgcgct gctcgaagca ggtgccgatg gcaaattgaa gtcgggagaa
    25621 ggaaaaacag ccttcgacta tgcgaatgac aacgagataa tcaaaggcac caatgtatat
    25681 tggcttctca atgatgcacg gttttgaaca tgcattgaga cagcatgctt gatagcaaat
    25741 ggattctctc gcatgcggga tgttcgatgt aataagtagc ttcgaagaag tacttgggtg
    25801 atcccaaata gctagggctg agcgccaggc ggtacagcgg tatttggcat ttgcccagag
    25861 ccattaccgg ccaattgggg agagtacgga tccgcaaggg ctcgatgata gtgcagaaac
    25921 ttttcgaagc ttacttagag gatccccctt cagcgtgata gggaagatcc ttctgcgccg
    25981 atctttgacg ctgccggaaa gaatgggata gcagaatcaa catgtcttct tgttcttcaa
    26041 cggaaaagac cgatcgtctc cgtggcgtct cacgaggatg caaggacgcg accggttcca
    26101 ttgcctatct tgcccggtga tcggctctac tttggccgca ggtgccgacg ggaatttgca
    26161 agacgaagac ggctggaccg cgctgatgat cgcagccgca tattgcgata acccagatgt
    26221 catccttgtg ttgcttgaag cggacgctga cggcaagctg aagtcaaagg acaggatgac
    26281 agctttcgac tacgcgaggg aactgacgtg tactagctcc tcaacgaggc gcgattctga
    26341 cgattccacg gctgcgcatc gtttgcacct agccaaatat cgccatcatg acccccgcgg
    26401 ccactgctga tgctatctgc cctgctacgt tggcgcccat ggcatgcatc aagaggtggt
    26461 tgttggggtc ttcttcaagt cctacttgct ggcagacgcg ggcggccatt gggactgcag
    26521 atatgccggc tgcgcctatg agcgggttga ccttgcctcc ggagagttta tacatgagct
    26581 tgcccaggaa tacgccgcag gctatgccgg cagagaaggc aaataggcct agaaatagaa
    26641 tctgcaaagt ctttaccgac aggaattttt cggctgtggc tgaggatccc acggccagac
    26701 cgaggaagat cgtgactatg ttcgtcagtt cgttttgggc ggttttgttc aggcgttcaa
    26761 ctgcccctga ttcgcgaaag aggttgccga tcatgagcga gcctattagc cctgcggtgg
    26821 acggcagaag aagtgctgaa actacggttg tgattatggg aaagagtatc ctttccgctt
    26881 ttgatacggg acgggactgt gtcatgatga ccgaacgttc ttcctttgtg gtgaggcccc
    26941 gcattatggg cggcatgatg atcggcacga gggccatgta ggagtaggcg gctacggcaa
    27001 cgggtcctag catgtgaggg gcaaacttgg aagtgatgta gatcgaagtt ggaccgtctg
    27061 cggcgcctat cactccgacg catgccgact cgagcggttc gaacccaagc agtaatgata
    27121 cgaagaaggt aatgaatatt cccgattgcg ccgctgcccc cagcagcatt gtggaggggt
    27181 tggccagcag tggcccgaag tcggtcatcg ctccgacccc gaagaagacg agcaaggggt
    27241 agagccctgt ctcggcgccg aaattaaggt agtacaggag cccaccaggg gtgtcgcctg
    27301 taggcggaga cgtaaggcct gttgcgggaa ggttggccag caacatcccg aaggatatgg
    27361 gaagaagcag cataggctcg aactttttct ctatcgccag gtacatcagt atcaacgata
    27421 tcgcgatcat caccaggtgt tgccacccga gacttgcaaa acccgtgctc ataacgaact
    27481 cgctcagtgc ttcggccaac tagaggatcc tccttcagct tgacaggtgt attctgcgcc
    27541 gatctgcagc gctgtccaga agaatgtgat agcaaaggta tcatgttctt ttttattctt
    27601 cgacagaaaa ggccaatcac ctccgtggcg ttcacaagag aacatgtgga ggtgattggc
    27661 ggtgcgtggc ggcggttcct accgcctgtt ctgccaaaag atcggttcta ctttggccgc
    27721 aggccgtcct ctaccctggt gtgcatggct gtccagcgat cgggcgaata ggaggcggtg
    27781 gttccttgca ccttgtggcc gtgggctcgt atttctctgg tctgcagttc tatttggtgg
    27841 gtatggtcga tgcttgtgtc agaggaaggt ctggtccatc cgcggatctg gtgagagtgt
    27901 ccgctgtcat cgaggtctgt ttcccccaac aacctgtggg tatgtcctcc cggccctggc
    27961 cacggaggcc ccgtcactat gtcgactcgg tgaacgtgtt cgctctcgct gaccgtggtc
    28021 ccgaccaccc tgtgaacgtg atccctaaat ccttctatgc caaaactcgt tttttccaga
    28081 tgagaaagca actcctctag caagtccgat cacctcgcta catcttatac tcttatcaca
    28141 agaggggtgt tgtgcccacg gcacacacag gacaatggcg cggaaaggag aaataccaaa
    28201 gcgtcaaaga tattccagat gggatggtgc tttgtatgga atacgacttc gatcaggtgc
    28261 ttgacagacg gaatactaac tgcgagaaat gggattatct tgaagaacat tttggcaggg
    28321 cagatgtctt gcccatgtgg gttgcggata tggatttccc ttctccccca gaggtggtac
    28381 aggctataca ggaaagggca aagcatccgt catacggcta cacggggact tctgattcct
    28441 ttttcgattc gatagtaggg tggatggaca ggaggcacgg atgggcgata gagcgtgagt
    28501 ggatctcgac tactcccggg gtggtccctg ggctcgcagc atcagtcctt gcttttacgg
    28561 agccaggcga caacgtcatt gtgcagtctc cggtatatcc gccgttcttt cgggtggtgg
    28621 agaacaacgg gaggaggctg ctcaacaacg aactcgtaag ggtgggagac cgttacgaga
    28681 tggactggga tgacctggag gcgaagctcg gcgaaggggc taagctcctc ctgttgtgca
    28741 gtccggccaa tcctgtgggg agagtctggt cagaggaaga gctggcgcgc ctttcggaaa
    28801 tgtgtgtcgc ccacggcacc gtgatcgcat ctgacgagat acacagcgac atcatttatt
    28861 catgggcaag acacactcct tttggcgccc tgagcccgga ggcggctgaa aactccgtta
    28921 cctttgtggc tcccagtaag accttcaatg ttccggggct ttcgacttct gttgcgatca
    28981 tacccaactc cgacttgagg gagggtttca atcgcgtcct tagcgggctc ggactcggaa
    29041 cgaatctgtt tggtattact gccctggaag ctgcgtatac ctacggagac cgttggctgg
    29101 atgacgccat tgcctatatt gaggagaacc tcaggtttat ggtggagttc ctcggccgtc
    29161 aggtgccgga gatccgagtg gttcctcctg agggaacata cgtgatatgg cttgactgtc
    29221 gggggctggg gctcgatccc gatgctttgc atcggttcat ggtagaggag gcgcgagtag
    29281 gcctgagcga cgggacattt ttcggtttag gaggcgaggg atatcagcgg ataaacatag
    29341 cgtgccccag gccgatcctg cgcgaagggc tggagcgaat tgcgtccgcg gcagtgagat
    29401 tgcggggcgg agtgaagggc acgaagacag gggcgtagga ggctcgatgc tgcggacgcg
    29461 tgaatcatgc cgggaggtga agatggtggc gagaaagcgt ccctggtttg tgatggttct
    29521 ccacagtcat ttgccgtggg tgatgggtta tggcagatgg ccttttggcg aagagtggat
    29581 ctatgagatt gcgtgggatt gctacattcc gttgatccgg gcgtttagga atcttgagcg
    29641 cgacggcatt cccgcaaacg tcacgctgag tgtcacccca gtccttgcgg agcagcttgc
    29701 atcggagagt ttcaagtcgg ggctcgtaga atacctgggc gacaggcggc gcagggtcgc
    29761 tgatgacagg gcagaattcc tcgcccgggg aaatacggat ctggcgagac tgacgatttg
    29821 gattgaagag cagatagacc gggctttgca ggacttcgaa gacatgggcc gagatattcc
    29881 cggcgcattg gccgaccttg ctgcccgggg ctgtattgaa ctggcgacat ccgcggcttc
    29941 ccacgggtat ctgccgttgc tcaaacatga gaagactaag cgccttcaga ttcgcatggg
    30001 gaaggtgggg tttaaggaag tcttcggcat ggagccacgg ggattctggt ttccggagtg
    30061 cgcctacagt ccgggcttgg aagagctcgt ggcgggtgag ggttacgctt atactgtgct
    30121 ggatgccatg gctgtgcagg gcgggaaggc aatgagccag tacggcgaaa gcaccagaac
    30181 ggtgccgcct acaggcaggt ctgtggagac ggtctacaag tgttgcaatt ctgaactagc
    30241 cgtcttccca cgccacccgg aattgtgcgc ccaggtatgg tccagatgga cggggtaccc
    30301 gggagacttc gcctatcggg aattccacaa gcagaagccg cgatctggaa tgagatacta
    30361 ccgcgtgacc gattccgcag gcgatttgcg cactaagaaa ccatacgacc caacggttgc
    30421 ggcagccagg gccagggagc atgctgcaca ttacgtgggg cgcatcgagg aagcagctga
    30481 tgagagccgg tcggaagtac ccatactcac gaccgcgttc gacacggagc tgtatgggca
    30541 ctggtggcat gagggcccga ttttcctgga agaggtggca cggctccttg cccaaagtga
    30601 cgcggtgaat catgcaaccc cgtcgcgcat ccttgaggag attgattggg aaaaggttga
    30661 caggatagag cccttggaat cgtcgtgggg cgttaattgc gaccatactg tgtggatgaa
    30721 cgagtgcaca cggccgatgt gggaaaagat atgggaactc gaagacatag taaacgacag
    30781 ctgggcattg actccttttg ggccgtggcc cggcgacttc tgggaattac agtccagcgt
    30841 tgatacattc ctccgcgaat tcctgctaat gatggcttcg gattgggagt ttcttatgta
    30901 tactgggact gcgggggatt atcctgaacg gcgctttgaa gggcacagga aaaactgcgc
    30961 agaggcctat ggcaggcttc gcgcggcttt gaaagatcat cagttgcccg agtatcttta
    31021 cgaacccttg tatgacagaa gggatgcgga tcttgagtcc cccgaaggct gttagccgga
    31081 tcagcggcgt tggcctctga tttgccctac actatatgca acacgaatta ctaagaaggg
    31141 ggactgtcta gcaatgagag ttctctttgc ttcttcggaa gtcaacccgt atatgaaggt
    31201 aggaggactt gctgacgttg ccggaagcct cccagggttt ctggctgaag cgggatgtga
    31261 tgttcatctt gtaatgccca agtacgagac tgccaaatgg aacgggattg cgtttcaaga
    31321 gtggggatct gtgtaccctc caatggggta tgggcgggaa gaagcacagg tctattattc
    31381 gaagatttct aggaatctga cgctctatgc ggtgcgcaac ggtgcctact ttgcccgtga
    31441 aaagccgtat gattcccctg acgacatgag aaggttcatg tttttcgcga aagcgtgtta
    31501 cgaattggcc agggcccttg aagtcgacgt ggtacacgcc aatgactggc ataccgggct
    31561 tttgcccgtg ttctgccggg tgtacggttg cccgaaggac cccggcactg tgataaccat
    31621 tcataacctg gcgtttcagg gagaagggga ctgggacgat tttctctatt cgtctctccc
    31681 gtgggataca tttaacccgg gtggcgttga atactacggg aaattcaata tcctgaaggc
    31741 tgcgatgatg tatgctgaca ggataactac ggtatctccc acttactcaa gagagataca
    31801 gaccccggag ttcggtatgg ggcttgaaaa ggttctgatg gcgaggcgcg acgcgatccg
    31861 ggggatactc aatggcatag actacgatga atggaacccg atgactgacg ctcttataac
    31921 atccaattac gatgttgaga ggctcgataa gcggatcgag aacaagagat cccttgcggc
    31981 aagattcggg ttggaataca ggcctgagac tcccgttttc ggctttgtgg gtcggctctt
    32041 tgagcagaag ggcgttgata tactgataga ggccactaag cgtcttgtgg aacaggatgt
    32101 tcaggtagtt gtcttgggaa caggcgctcc ccagtatgaa gaggcgctga ggagcctatc
    32161 ccatgcgcat cccgggaagg tcggcgcgaa gctggcgttc agcaatctgt tggctcacga
    32221 gatttacgca ggggcggatt tcttcctcat gccgtcgaaa ttcgaacctt gtggcctcgg
    32281 gcagcttatt gcaatgagat atggagcaat tccgatagtg agggcagtgg gcggtctgcg
    32341 tgatactgtg actgagcccg taaccggcct cgtcttttgg gagtattctg cgtcagaatt
    32401 gcatgctgcc atggaaaggg ctctggaaat atatgataat cccgaggcgt ttctcgctat
    32461 gcgcatccgt tgcatgacag cggattattc ctggggaagg tcgcgggacg agtatgtctc
    32521 gctttacaag gagatcgcgc ctgggcgata gttcctccgc aacacggggg ccaattatac
    32581 ggttgataag aaatgcttgc ccggaggcag ggtggcggcg aatcgtattg cgctgctgtg
    32641 agttaacctg ttgagcctcc gggcaagact cgatttgtag cggcagcaaa cccatcaact
    32701 caaccccgaa tccaacagtg aacaggcgca cgcgcagaat caagagtcga atagcttgga
    32761 tgacgatcaa accatcgcga aaagccagaa gacgtgcaag catgtaaccg ccctaaccag
    32821 taagcagacc gtttcctatc cgcagcttgc ctgcaacgag cgctgtcctg agacagtcgt
    32881 ccgtaaagac caggttgaca aagaggagct caaaaggctc gtgcacagca tatccagggt
    32941 gttgcctcca gagggtgaaa tcgtgaccga gatgggtgtc gcgttagggg ctatttctat
    33001 cgagcctgac agatttggat gggccagttg ctctcagtat gttgccatag tgccgacgct
    33061 tccggctctt gtgttgagct gccaacacct cgaccgtcct tggtcttggc tcgtgtggtt
    33121 atgggttctg gggagcggag ccggcgcgtt aggtggtggg caaggctcag ccttccatgg
    33181 ccggttttcg ggagggcggg gcctctgttc ctcgatatta agttttgtca tgtttgggct
    33241 gggcgtcctt tctgtctaca tgaacttcta ggagatgggc cgtgggggcc ttcgcagtaa
    33301 tgtggcattg gctgctggcg cggcccatcg agttgtagtt attcggacgg tggaaaggat
    33361 gtggtctatt tgagggcatg gagagggatt attgaggagt atagagagta tctcccgatt
    33421 gaacgagaca tgccggtagt gacgttgctg gaagggaata ctcccctctt gccagctggc
    33481 aatctcgccc agcgacttga agccgacatt gagatatact tgaagtttga aggcttgaat
    33541 cctacgggct ctttcaagga tcggggtatg actcttgcgg tctccaaggc tgtgcaggat
    33601 ggggctcctg tcgtggtctg tgcttccacg ggcaacactg cggcttcggc cgcggcctat
    33661 gccgcaaggg cgggcttgcg ctgtattgtc gttattcctc atgccgctgt tgcctcgggc
    33721 aaacttgccc aggccagggt ccacggtgcg cagattgtgt ccataagagg aaacttcgat
    33781 gttgctcttg ccatagtgag ggagctggcg gctgagaccg gcatcgtctt ggtgaactcg
    33841 gtcaacccgt ggcgcatcga ggggcaaaaa actgctgcgt tcgagatagt gaacgagctg
    33901 ggggatgctc ctgattggct tgtgattcct gtagggaatg caggcaacat aacggcttat
    33961 tggaagggat tcaacgagtg cagagttgtg gggatggcct cgcgatgccc tcgtatggcg
    34021 gggtatcagg cgaccggggc cgctcctatc gtgctaggta tgattgtaaa aaatccccat
    34081 acacttgcca gcgccatcag gataggcaac cctgccagct gggcgggggc aaagacggct
    34141 gctgacgatt ctggtggttt gatagattca gtatccgacg gcgagattat ggaagcttac
    34201 agcttgttgg ctgcggtcga agggatattt gccgagccgt cgtcgtgcac atcggtggcg
    34261 ggggtgatca agatggcgcg attgggtcgg ttcgaaaagg gacagagagt tgtggcggtc
    34321 ttgacagggc atggtttgaa ggacccggag gcggctatgg cgtgcgacgc ttattctctg
    34381 gccgggcgat ccccagagga cgggagccgg gctgtagaag gtgtagtcga tgctgacgtg
    34441 gaatctgtca gaggattgct gggggtttga acagtcatca taagaggtgt attcctatgt
    34501 ctgttaagaa agtgatcgtg catgctccgg cgacttcggc caatatggga ccggggtatg
    34561 actctatggc catggcgttg gagcttaggg atgatataac attcgagctc gcgggagatg
    34621 gcgagtctca ggctattacc ctgtcggaag atggaagccc gtctcctgag ggccttccta
    34681 ccgatcggtc gaacttggcg atacgcgctt ttgaagctgt attcgttgag gcgggcgaga
    34741 ggccgccatc tgttcgtgtt acgacggtga accggattcc aatagcccgg ggcctcggaa
    34801 gcagtgcagc ggcgattgtg gctggggtga cggctggggg cgttatccgt ggtagcgaga
    34861 tcgatctgca aagccggctt aaccttgccg ccaaaataga agggcatccg gataacacag
    34921 ttgcctgcat actcggggga gtgactgttt cgtcgctggt cggctcccgc gtgatgtggg
    34981 ccaagattcc gctgcagaga gagctagggg ccttggttgc agttccggac ttcggggttt
    35041 cgacggggat cgctcgcaat gttttgccgc agagcgtgct ccatgctgat gccgtgttca
    35101 atgtgggcag ggctgcgctt ctcgtagcgt cgctgtgcac cggggcttac gagaatctga
    35161 gatatgccac cgaagacaag ctgcatcagc cgtaccgttc tctgttggtg cccgggatgc
    35221 tcgacgccat acggtcggcg tgcgatggcg gggcactggg ggcttatgtg agcggggctg
    35281 gacctgccat attggctttg atggactcgg aatgtgatcc ggagccgatt gcgcaggcga
    35341 tggccgctgc ttttaaccgt tcccgagtta aggtcagcga tatgatggcg ctttccttgt
    35401 ctagcacggg ggctgtttgg gaggttgagg aatgagttcg cgttcggacg gaacgcaaac
    35461 tggagatggt tctgttccag tctattttgg ccttggatcc aatctcgggg ataggctggg
    35521 aaatctgagg tgtgcagcgg caatgttggg gcaagctgat ggtctgtcac aatttgcggt
    35581 gtcccgcgta tacgagactg agccgtgggg ctttgttgac cagccgcctt ttttgaactg
    35641 tgttgtgcgg gcacacactc tcctcgagcc agaggttgtc ctcgctgtga cacggtcggt
    35701 cgaagatgct ttgggacgcg agaggagtct tcgctggggg cctcgcatta tcgacgtgga
    35761 catcctcatc ttcggcggat tggttatgga tactgaggat ttggtcattc cacatccgcg
    35821 gatgtgggag cgggctttcg tccttgttcc cctcatggat ttggcgcctg acatgatcac
    35881 tcccgacggg gccactctca gtcagaaggt tgccgacctt ggcgatgcag gcggggtgag
    35941 ggtcttcgac ggtgatctcc aaggagtttt cgaccgggcg gagaatatca tgatgtgaag
    36001 gccggggctt tcggacgcct ggcctaacct ggaaaagcgc tgggaggtcc tttgcatgtt
    36061 gcatttgggt gttggttcgc accgcaattt gcgggctctt gctgtagcga tcccaatggt
    36121 gctggcgtgc gctgcagtct cctttagcgc tgggggcgag agggaatatg caaaccgcga
    36181 gcacgggttt gttttcaggt atcctgatga ctgggagagg caatcaggca gtgtagggtc
    36241 atatccgatc atagcctttg gcgaactgcg gaagacgggc tttgcggcct cggtcaatgt
    36301 agtggtggca acgcaaccgg gagcaagcgc taagctgtac gcgcgcaatg cggctgctcg
    36361 gatatctcag ggcggggatc cgcggctttc tgatttccgg gaagacggtc ccggggtgcc
    36421 tggcgctgtt tacggcgggg attcctggtt gacgaggttt tcgtgttact cgtctgatgt
    36481 gaggaatagg gtgtggtttt accaactcac ggtggttcgg ggatctgatg tgattgttct
    36541 cactgctgca tgtctcgaat cggagaaagc tgcatacctg cctgtgttcg agagaatagt
    36601 atcgagcttt cggcttatct gacggggggg tcccgtgtgt caatggagta taaagttgtt
    36661 gattttgaaa agaaaaaggt tgagataagg ttcgggcagc ttgctcagga ccttgtcgat
    36721 tttgttaacc tccctgagtt caatggcact gcagccaatg gactccacaa gttcatgtcc
    36781 gaggtgatgg aagagccttg ggtggacttg gacgatgatc aagacagttt ggaaaacgag
    36841 tttgttgaat ggctcattct ggactaccaa gacgagatca caggggtttc tgtggtccgg
    36901 gcttacctcg attccgccga tgagtgggag ttggaagact gggaggcgcg ttcgttggaa
    36961 caatgggccc ggtctgtttt ggccgtctac gaggttcagg agattctatc cgatgacagg
    37021 gttgttcttc gtgacgtggt gacaggtaat acgtacagcg tgattgcgcc gggcataggc
    37081 ttgggagtgc tgcgatggtc ggccatgctc gcgcgtctcc tcgtagtgga tgatctgtat
    37141 actcttgggt cttctacgct tgtaataccg aggcctcttc tcaggtatgt gctgaagata
    37201 atgggccatg tgtacaggac gctgcgcttt cagggatatg atggagattg ggagagcttt
    37261 ctcaaggtct ctacgctcag tctccgcaaa ctggttcgcg tgatgctgat ggcgtggggc
    37321 gtgcaagaaa gcgaggacgc tgtagcgctt tattgtgatg gcttgtctgg gtcttctagt
    37381 ggtgaatttg cgggagtccg gggagcctgt tcgcccagcg aagcttcagc ctcaggcgcg
    37441 cgcaagcttt atcgatcagt ggtccgcgta tcagatgcca agggggctct tgacgctgtt
    37501 acacgaagcg gcacagggat tgcgttgtct agctcttggg tggatgaggc gggtgctgtc
    37561 tgtgcggagc ttgcgtgggt cagagagccg catgagagtg acccatctgg tgtggcgcca
    37621 tatggcgctg acgatgtggc ctttgttctg ttggctgacg gtgctttcac tgtgtacgcc
    37681 gacaatcgga gcgctttggg aagatgcgcc agcattattc ggaggagcgt agcccaatac
    37741 gtgattccga ggcagccggg gaagaaggca tcttcaagga agccgccaag cacacaagag
    37801 agggcgcggg cacatgctag gatcgaccag attgcccggg aagccattgc agagcactac
    37861 cggagttggg ccgattcccc tctcccggtt cttcaggggg aaagtcctcg agacgctgtt
    37921 gcttcgggca tcgggcagaa gatagttgag gaattgctga aggatatcga ataccacgag
    37981 aatctcaaga ggcgcgcggg attgccgtgg gttgatgttg acggcctgtg gcatcacgtg
    38041 ctatcagccc aaggctgtga caatgggcta agcgccaagg agcattcagt agtgggtttg
    38101 ctcgacgaga tgatgaggtt ggacggctac acagaggatc agatatttgc cactcaaaag
    38161 atatggtgtg attatgcttt gtccgaaaac ccaaatgtgg tgcatgccaa tacttgggca
    38221 gctgcggtag agtacgccgc ggccatagct caggatgaac ctgtgaccca ggccagtgtg
    38281 gcccggaaat accaggtgtc agtctctacg ctctcgcaga ggtaccgcca gattttagac
    38341 agcatcaatg ccaacgggta gcatggtgtt atctggaaag taatagagat caaacagggg
    38401 cggtttgtcc gccctccttc tttgtctgat aagcttgagc agccccttgt ctgttatcca
    38461 tggccacaac ctgtgtgtat ccccgagcca cgcagtgcct agcccgtcca acggggcgaa
    38521 agccgcagct tgaatcggag aactccgggt cgaagaggga cgtgagaatg tggtgaatga
    38581 gccggccgaa aatcgttggg acgccaagca tcatcacgcc acagtacggc actccggtag
    38641 tctctccacc gcacgatccc cacttgtaac gtctgccagt tcaaccgcga ctctcagtgt
    38701 gcccgcctca tataccgcaa cgctcagacc ttgccattgt agttgctgaa cgtgccgggt
    38761 gcatggaaaa gaggcccggg gcggttgccc cgggctcatg ttattcgttt agatcttctg
    38821 cgcctggcga agcgagttgc cctatttgac cggcacgatc cttatctcgg aagaggcctc
    38881 aacgctctca tgttccataa ggtatcctgc gacgatgtcc ctgagcattt cgccgctgtc
    38941 gaagataatg ttggcctcct tgaaccatac atagccgtcg ccgcctgctg ccatgaaatc
    39001 atttgtggcc acggtgtaga gtcggtcggg atctaggggt tcgcctgcta cgagcacttc
    39061 caggatgcgt gatccggcgg gcttcgacgg gtcgaatctc aaggtaagcc ctgaaacctg
    39121 agcgaaggaa cccgcttgat tcgggtagct gctcagtccg tgctctaatg cggccaggat
    39181 cttgtcgccg ctaagctcga tgacgacgag ggagttgtcg aagggcagga cagtatagac
    39241 gttgcccatc gtgatgtcgc ccgcggggat gctagctctg atgccgccgc cgttggttat
    39301 gacgatgtcg gcgccggacg cttctctcag ggcatcggtg atcaagttcg acatgttggt
    39361 tgtgcgggtt cggacattgg cgcgctcgcc gtcgagggcg actttggtgt gtcctactac
    39421 ttgggccatt ctgtcctcga gtgcggcatt gtactttctt atgattgttt cgatgccgga
    39481 gtcaggcgcg gagtttggcc gggatgcgtc catcgcaacc agcctgcctc caaaactctc
    39541 gatcttcccg tcgacgactt cgatttccaa cactccaagg tgctttgccc attcaccggc
    39601 ctggaccact atggcacccc ccacaagttt cggctcgggg acctcggtgt gcgagtggcc
    39661 gccaacgatt atgtcaattc cagggaccgc tgcggcgagc ttcaggtcag tgtcgtatcc
    39721 aaggtgtgaa aggcagagaa cgatatcggc ctcggcccga agctttggaa cgactgcccg
    39781 ggcagtctcg atcgggtcgg cgaacgagag gcctatcacg tttttcgggt gggtgacgat
    39841 gggggtctca accgggctga gaccgaacat gcctatcttc acgccgccga tttcggtgat
    39901 gaacgagttc tctacgaata gctcgcctga atctgcgtag accacgttgg aggcgagaaa
    39961 tgggaagttt gccgcggact ccaggtcctt caaaacttgc tgcccgtagt tgaactcatg
    40021 attgccgaga accgtggccg caaaacccat atcgttcatg gcttcgatca tgggcattcc
    40081 gctgaagagg ttgacgatat tcgttccgtg gaccatgtcg ccggctgaga gcagaagcaa
    40141 attggcttcg ggatccttcc ttagctcgtc gatgtatgcg gcgatcttgg tcattccgcc
    40201 aatggggtca gtcccgtctg atacaaatgg ctccatgcgt ccatggacgt cgttgacatg
    40261 gagtatggtg atgtgcgtgg tgccttctgc tgcaaatgcc gatccataga gtccgaagac
    40321 tgtcagaagc gcgatgagga caaagactat cgagagattg agtctggact tcttgggcat
    40381 gatgatcctc cttttttagg cacagtagtg caagatgtta attggaagtt tcttcgtgaa
    40441 cgtgaaaact cctctctata catacaatgg cagggatatg ccttgcttct agggcgatta
    40501 aggggctttg tgccatgaaa tcgcaaaacc gccgatttta gcacactttc tttgtgaatc
    40561 gtcgaaggag tttgcgttgc aggctagaat atgaagggca agaccttgtc actgaggagg
    40621 ggtataaatg aaaaaggcat caattatggg actagcccta gtgttggtcc tggcggccag
    40681 cgcgtttgct tccgcggcca attatgtcgg cggcgggcta ggaatcaagg cggcagctga
    40741 tgcaagtgac gcaaagggga tcataggcgc tctcagcctc gactttgacg ttatggagaa
    40801 gctgacgctc tctgccaatg gctacatcga gttcaagcag aaggttgaag aggagcctcc
    40861 ggccaccgac gcggcgctca ccatgccgaa gttcgaggaa cccacgtatt ttgggggcgt
    40921 ttacggcaag tatgcactga ccagcttcga tgcgctgaag ctcggcgcgg ttgcgggagt
    40981 caacttcaac aacggattca tcgtcccaaa agaggacgta gaatctggga cagacgagcc
    41041 tgctgctgaa cccagcaaag tcgactatgg cgcgggcatc tttgccgagc agcctctggg
    41101 cgagacgggc accatctatg ggcaggccat gtattggatg caggagaagg gcgtaggcgg
    41161 gcttggcgga atcaacttca agctcaccga caagatcgcg gtcaagggcc agatcgagta
    41221 cgtcaagaag cagcctctct tctcgatcgg tgtcggatac accttctaga ggcccaggaa
    41281 cgggctactc tcgagagcag ctattcttag gcagttactg ggccgcccgc gcgggcggcc
    41341 ctctctattt tgttaatgga attgcttgtt aacagaagca cagaagcggc aacaatgtgg
    41401 gccgctggca tacgaacagc aaagggggag acatcatgaa aaagtatgca gtgccgatta
    41461 tcctggcttt tactcttgtt attgcaagtg gactggcctc tgcgtcacac ctctctgcag
    41521 gctatatatc ccgcgatggg caactcaact tcacgcttgg aggcaggtac gatctgggcc
    41581 ggggttcgtc tgttgaaggc tcctacacgg tgcttcccgg ggggtatgat gtggcggcaa
    41641 ggtacaagat ggccctttac aggagctctg tagtggcagg cccggtcttc gagggcaggc
    41701 tcatcgggga tcatgtttac acctcacagg ctctttcggc gggggttttt gctgagaaga
    41761 tcggggtgac agg
//
LOCUS       DAOSYL010000012        41712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_767_length_41712_cov_8.181218, whole genome shotgun sequence.
ACCESSION   DAOSYL010000012 DAOSYL010000000
VERSION     DAOSYL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 41712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41712
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_41712_cov_8.181218"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..979)
                     /locus_tag="PLU15_03585"
     CDS             complement(50..979)
                     /locus_tag="PLU15_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO30036.1"
                     /translation="MLQGADQTVSSGEYDAMIIGAGPAGLSAAVNACVRKKSVAVISR
                     RLTSPSLEKAPWIENYLGVERISGRELSARFVNHARAAGAELIESDITGVFNLGDSFV
                     ALGSDGEFSAKAVVIATGSVQEASIPGESELLGMGVSYCATCDGPLYRGKKVVVLGYT
                     EHAIEEANFLAQICSEVTYVMARSVEPGDGPAIAEGIRVVKKRVLGIRGEDKVEEVEL
                     DGEAIPADGVFVIRDSIPVERLLEGVRVADGAIVVDRDMATNVPGVYAAGDCTGRPYQ
                     IAKAVGEGLVAGLSAARHVDRMAAKHLPKSLLL"
     gene            complement(963..1817)
                     /gene="folD"
                     /locus_tag="PLU15_03590"
     CDS             complement(963..1817)
                     /gene="folD"
                     /locus_tag="PLU15_03590"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOO30037.1"
                     /translation="MPATIIDGKAVAASIREDAAARAAEYREKFGRAPGLAVVIVGDD
                     PASRVYVNNKKKACAGCDIYSEEHALPATTTEDELLDLVRRLNADPKIDGVLVQLPLP
                     NHISEDKVIEAISPSKDVDGFHPINVGSLMVGKPALRPCTPAGIMELIHRTGIELKGK
                     RAVVVGRSNIVGKPVSIMLLAEHATVTICHSRTEDLPSVCREADVLVVAVGRPKMVKG
                     DWIKPGAVVIDVGTNRVGGKKLVGDVDFDEAVEVAGAITPVPGGVGPMTIAMLMKNCV
                     EAAFAARS"
     gene            complement(1972..3090)
                     /locus_tag="PLU15_03595"
     CDS             complement(1972..3090)
                     /locus_tag="PLU15_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30038.1"
                     /translation="MAAILFFICGLVLSLGSIAEPTQTGNSWFLPTGSSPSAPVGPVL
                     SLDIDITNDPGEEVATSHWTSPVEGYLELHGKAGTGGQGGYKLLARVDAGPVVGLEKI
                     VLDCGYESSPSQESHRTSDSKPERASESERTTSDSMCATLILVQANYDQSVGAMSKTT
                     VSTAYAWNGRTLVEVWSATTFSEAVWNLSWGAVGNVKANSWRWGVARERALRDFTSSG
                     GIPATAAKGFDLGPGNWVRLASSSTVQFSGGPVPSVVMESLHEYHSYSEKDDAFKLIS
                     SRKVTQTFCWSDRWKTFVLGEGTIGREGAIGMEYPGADDGLRIDPGTDVAILESERKC
                     VEGLVFGPPFLLRVKLPNGKRCFVSEDSVLTSAVESMV"
     gene            complement(3159..>3361)
                     /locus_tag="PLU15_03600"
     CDS             complement(3159..>3361)
                     /locus_tag="PLU15_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30039.1"
                     /translation="RSASKACRLGMTAPDIVNVSEAIIDESFAPDRSRIYSVLSCRGR
                     ITCAEKDILAFDSLMHQVHGPG"
     assembly_gap    3362..3461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3507..3899)
                     /locus_tag="PLU15_03605"
     CDS             complement(3507..3899)
                     /locus_tag="PLU15_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOO30040.1"
                     /translation="MNDRVWSIWICRARSNLARAKHGKFSEDVLYEDLAFDAQQAAEK
                     SLKALLLYLGMDAPRTHSIGYLLKLIEDGSKMAIPPHVQEAAVLSDYAVTTRYPGNYE
                     AVSEEEYLRAVELADDVCSWVMSIIRQC"
     gene            complement(3889..4191)
                     /locus_tag="PLU15_03610"
     CDS             complement(3889..4191)
                     /locus_tag="PLU15_03610"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO30041.1"
                     /translation="MDVLSAIVKIIVSTADPDKIILFGSRASGDQSPDSDYDILVLKK
                     GDYHKRTLAQQLYRELAVIPAPIDLVVEDPDKAEQLRDVPGLIYGEALRGRVLYER"
     gene            complement(4582..6033)
                     /gene="murD"
                     /locus_tag="PLU15_03615"
     CDS             complement(4582..6033)
                     /gene="murD"
                     /locus_tag="PLU15_03615"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOO30042.1"
                     /translation="MLTDNQKRVMGTLARKRVTVVGIGITNVPLIRFLAQAGAKVTAC
                     DIKTADQLGSELASLSGLGIDVSLGPGYLDSLADCDIVFLSPGVNPEQPQIMAARDVG
                     VSFSSEIELVLKLSAAPVIGITGSSGKTTTTTLTGRILAEDARLHHPERDVLVGGNIG
                     TPLIEHVLSVESEDLVVLELSSFQLRLLAVSPHIAAVLNITPNHLDVHPNMEDYIFAK
                     ENIVRFQSEDDWAVFGEENEITARMATRARGNVLMFDASGPVDRGAFLDGESLVLRCV
                     ADGVCKDTIVMERSDIKIPGMHNVRNVLAAIALCSAVGADPSAMRHAVSSFEGVEHRL
                     EAVGEVGGVAYINDSIATTPTRTIAALNAVERPIVLIAGGYDKNLPFDEMAEVALGKV
                     HTVVLIGVTSDKIEKALVEAARRAESEPPRIVRARVFEEAVDAARASASPGDVVLLSP
                     ACASYGMFRNFEERGRTFKEIVGRYVEVAGCKE"
     gene            complement(6149..7294)
                     /locus_tag="PLU15_03620"
     CDS             complement(6149..7294)
                     /locus_tag="PLU15_03620"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOO30043.1"
                     /translation="MKVVIAPDSFKGSLAATEVCRAAEEGLRRVWPDAEVVRVPMADG
                     GEGTVEALVDATGGQVIDVEATGPLGDKVQAFFGILGDGTTAVIEMAAASGLPLVPLD
                     KRNPMTATTRGTGELIAAALDRGCRKFIIGIGGSATNDGGAGMAQALGARLLDAEGKE
                     IGPGGAELARLTRIDTSGMDRRLVGAEFVVASDVDNPLTGPNGASAVYGPQKGATPEM
                     VQVLNAALANYASVIRRDLRIDVEAMPGAGAAGGLGGGLVAFLGARLEPGVEIVIETT
                     GLKEKVSGADLVITGEGRIDFQTLYGKTPMGVARVATECGVPVAVLAGSVADDARGLH
                     DHGIDALLTVVKGPCDLDAAVRRAYANVADAAETLARLIEIGRRQRG"
     gene            complement(7424..8725)
                     /locus_tag="PLU15_03625"
     CDS             complement(7424..8725)
                     /locus_tag="PLU15_03625"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO30044.1"
                     /translation="MHRKAGIAPPQPVVIVVEKLKSCGHTAYIAGGAVRDMIIDQRRP
                     VDWDIATSATPAEVSSCFEHVVPTGLAHGTVTVIEEGMSIEVTTYRHDGPYVDGRRPE
                     HVVFLHDIAADLARRDFTINAMAFDPTTGELVDPYGGVSDIEARLIRCVGDPAERFSE
                     DKLRMLRAVRFAATLGFSVDSSVVDAARALAPGIVQVSVERIMDELVKTVRAPKPGAA
                     LVLMEDMGLAPYILPELCCEVGEEERKALYAACDRLHPEPKERFAALLSPLGPNGARI
                     ALARMRVSNQFSITVSRIIEGAQNLGQNPSDYELRLTASRVGQDCLLPAVRLNRALCR
                     QGTGSLAVADLGSEGIEAVESLEERAGSVLAAKPALRIADLDISGKEVMEATGLSQGP
                     RVRIVLQRLLDQVLHDPSANKKECLLEMATGIAEEMEQELA"
     gene            8888..9517
                     /locus_tag="PLU15_03630"
     CDS             8888..9517
                     /locus_tag="PLU15_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO30045.1"
                     /translation="MACEWVIQLSNEYAIARPAQPVSKATEFESLVRRHQNSVYNIAL
                     RMSGDPEEARDLTQEAFVRAYRSFDSYKRGTSFDKWLYRIVTNLYIDELRRRKRRPYI
                     ESLDQRITTSEGDELDKEIPDMSSNPETVYDRDHLDAFIQDALDSISPEYRMAVILCD
                     IEGFSYEEIAQIMGTSIGTVRSRIHRGRRALRERISPYLEERSREVSQK"
     gene            9514..10143
                     /locus_tag="PLU15_03635"
     CDS             9514..10143
                     /locus_tag="PLU15_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOO30046.1"
                     /translation="MTCNMAERLISAYIDHELSEDDRNRVRQHLAVCSSCMATYTATL
                     EVKRALSSMTPAQCPECVWEKVERQITLIPFDREMKRDDSTKPAWSFIKLVIPAAVAG
                     TLIVIPLVQALLGISLFGAPDKSQPPIASSKPNPQLELTSPSVRIHAAGTFSSSDALE
                     EVLGENIWLLEPTQASGKAANASQHGLDLRDIYDDYFMQVTSPERRYGR"
     gene            10140..11291
                     /locus_tag="PLU15_03640"
     CDS             10140..11291
                     /locus_tag="PLU15_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO30047.1"
                     /translation="MKASFKKYLPLVVIAIISSVITSIVVVSYGFPERKGDTAAAVTP
                     IVASSYSGNLPSIADVAETVGPAVVRIDTERVVSYRSPFPDDGFFGPFFRDFFGGERI
                     QPGTGSGFIISPDGYIVTNYHVVEESRKVEVTLTDGRKFTARVVNGNKDADVAVIKIK
                     ANNLPVAQLGDSSKLRQGDWVVAIGNPYGFDHSVTAGVVSAVGRRIDDESGGTLATGD
                     LIQTDAAINSGNSGGPLINMEGKVIGINTAIIPYAQGLGFAISINSVKDTLKDIVNYD
                     EGPTPWIGIWYQQLDDDIAKQLGLSDANGILVTNVIQDSPAEKAGLKEGDVIKEIDGK
                     QITAKMSLAEEISKKKVGDKVVMYVLRGGQKHYLTVVLGEYPGDTLMLK"
     gene            complement(11617..11886)
                     /locus_tag="PLU15_03645"
     CDS             complement(11617..11886)
                     /locus_tag="PLU15_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOO30048.1"
                     /translation="MNDEQKRDVINRLKRIEGQVKGVQRMIEEDKCCADTLDQIAAAR
                     AALYNAGVLLLERHARDCVMEAMAEGGEGEVIDELVRVIKKFARS"
     gene            complement(11903..12292)
                     /locus_tag="PLU15_03650"
     CDS             complement(11903..12292)
                     /locus_tag="PLU15_03650"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOO30049.1"
                     /translation="MGGRIKVIGVFEPEEDWHLEDEDGQRLEGALSPVAHLVASLVGC
                     SGLTMRGVMDKMKIEHQGIVIEGIAAISDERPTFVRSIWLEVSAPGACINEAQFASIL
                     EMTEKYCLIAQTFRHGPELILSAKTIP"
     gene            complement(12429..12896)
                     /locus_tag="PLU15_03655"
     CDS             complement(12429..12896)
                     /locus_tag="PLU15_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOO30050.1"
                     /translation="MRELDLHGMKTADAMSFFMREYDNALESGSSSLKVIHGYGSGGV
                     GGDTRRCIRALLSANPACVDYVAGEDVDGNPGYTVVYPKRRLPSGATGLYDSIIQFCS
                     SPKTKSAIVRRFVRRTPESEIDRALRDLENRGRLRTFFRNGRKHYIDSRSGRV"
     gene            complement(13045..13482)
                     /locus_tag="PLU15_03660"
     CDS             complement(13045..13482)
                     /locus_tag="PLU15_03660"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOO30051.1"
                     /translation="MAKTTFSASMAWSGSGMYCEGGTRGFKVEVDEPKALGGTDRAMN
                     PVELLLCALGGCMSICAAAFAGACGVDLQGFSVEVEGDLDPDGFLGKNPNVRTGYQGI
                     RYRMIIDSPSPQENIDRLVETIEKRCPISDTLRGVDVVRSAST"
     gene            complement(13657..14151)
                     /locus_tag="PLU15_03665"
     CDS             complement(13657..14151)
                     /locus_tag="PLU15_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30052.1"
                     /translation="MANGSFDDWDSANEPSGWNIAGNVGEETTNVHTLGFSANLGTPT
                     GVDNPAKLGQTLTEVKCGCCYRFEFHATGHNNSDDAFRAQVLFDGSVVTEVLLPSPVE
                     PQYSYFSAYVPCVPPGVTVDILFIKPGAGTWFIDDVGFIAEGPCTGGYWHPFGGCDSG
                     VSTV"
     gene            14163..14504
                     /locus_tag="PLU15_03670"
     CDS             14163..14504
                     /locus_tag="PLU15_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30053.1"
                     /translation="MDKCEPADQADPVDPVDQGDPEDQANPADQEDPVVPVDQAVPVY
                     PADQVDPADQAVLADMADMANTVSTVDTVDNRDYPHRMAFWVFGTTTYRSPRIEYLIQ
                     CIQQQRHADQD"
     gene            complement(14798..14873)
                     /locus_tag="PLU15_03675"
     tRNA            complement(14798..14873)
                     /locus_tag="PLU15_03675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14838..14840),aa:Thr,seq:cgt)
     gene            complement(15063..16223)
                     /gene="pgsW"
                     /locus_tag="PLU15_03680"
     CDS             complement(15063..16223)
                     /gene="pgsW"
                     /locus_tag="PLU15_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOO30054.1"
                     /translation="MRKGCMAPTGRQYGRDRVNFGILLLASLVALAALAVSVRPDCIG
                     LPGDPRGSVGACVEEQIAAAEIMAECIAAVRDARAELGIAIDPDLDPNGTGLIGHDLT
                     PLTTTVGVLEAKRTATSPDFAALMVKYFNEAGLREGDVVAVGASGSFPSLIIATLAAS
                     RAMNLEPVLIYSIGASMYGATIPEFTFIEMLDAIRANGILPYYIAAVSLGGDDDAGGG
                     GLFEESRDISEEIAERSGAYVIREDSIEKSVARRMQVFEEAAAEHGRPIACFVNVGGA
                     SANYGATSASLDFPNGLVMVPKVMSVDPQRGLMFEYAAMGLPVINLIDIRGLAIQNGI
                     PVDPIPLPGPGEGRVYAAHSCSKPAAAIGLAGCLFLIGGAWAKSRRRLQPDV"
     gene            complement(16387..16818)
                     /gene="pgsC"
                     /locus_tag="PLU15_03685"
     CDS             complement(16387..16818)
                     /gene="pgsC"
                     /locus_tag="PLU15_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOO30055.1"
                     /translation="MTVTVSIGIGLIVGFIFTELTGLYAGGLVVPGYLALYLDQPLRI
                     LATFLVALLAYGIVWVISNFLAIYGRRRFMACVIVGFWLGWLLNRTGMSITPGGPQLR
                     AIGYIIPGLIANDIARQGAARTFLSVLVVAVVVRLLMIVFA"
     gene            complement(16815..18002)
                     /gene="pgsB"
                     /locus_tag="PLU15_03690"
     CDS             complement(16815..18002)
                     /gene="pgsB"
                     /locus_tag="PLU15_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOO30056.1"
                     /translation="MHGFAVMCACLFVAGVIENWLHTRRLSRIPVRIHVNGTRGKSTI
                     TRLIAAGLRAGGMRVIAKTTGTAARIIFEDGSEEPVVRRGNCANISEQMRLVKLAESR
                     GADAIVVECMALQPENQWAAERHMIKSTVGVITNVRDDHLDVMGPTVHDVAEALSLTV
                     PYGGALVTAEQKFLHVFEDRARKTGASVYVVDASGAPADATANFDYISFPENIACALK
                     VCELCGVPRDLALEGMLTAQGDPGATRILRIDRGEASYVFVNAFAANDRDSTELVCKA
                     AEGRIAPFIRKGLPAIVILNNRSDRPFRAEELAGFVAESISPQWAFVVGEAGRVAVRG
                     LKRGKVAESVIVDLTHQPKAERILAKVADRCPAGAVIYGIGNTRGMGERIIQYFEANG
                     VAL"
     gene            19279..20859
                     /locus_tag="PLU15_03695"
     CDS             19279..20859
                     /locus_tag="PLU15_03695"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO30057.1"
                     /translation="MRTKSKYTGLLLAIVMVIASAFPAFACTSVPVSKGASADGSVMT
                     AHTCDGWYDARTYVVPGKTHKPEDTFKCYRDHLHADYAEPELIGEIPEAPVTYTYFHA
                     GYPYMNEHQVIMGETTIGNRSELSTDIGLLYIDTLQILGLQRAKTAREAVQIMGELAE
                     TYGYIDGGECLTVGDPNEIWHFEVMPPGNFQKGAIWAAVRIPEGHVGVSANRSRICEV
                     NPDDPENYMCSSNVFDVAIENGWWDPESGEEFLFWKAYCPSNWFGCKLREWRVLSMVA
                     PSLNLDPNADRYPFTVKAERPITVQDMMNIYRDHYQGTEFDMADAPGWYLKDAEGNPV
                     VDKQGNPVKTPYAQPNPGTARRQHIGIPVNMRNIAVPYCSYFTIVQARDWLPDPVGGL
                     CWFGLSNPLSSVYVPIYCGVDSLPKNYAVNDRKVFTFDSMKESAWWVFEYMDNLVSRR
                     YVDILADLNKVREPFEAEQFELQPVIEKAAVELYEKDPALAKTFLTNYTHSRCAKAVD
                     TYYRAAEELTLKWNDNTF"
     gene            20990..21622
                     /locus_tag="PLU15_03700"
     CDS             20990..21622
                     /locus_tag="PLU15_03700"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOO30058.1"
                     /translation="MKHSSVRRTILIAIAAVMSMILSTGVCFGSVSKPAGIEIPAKLP
                     RLDQPVLITSIGQAPGASWAKVLMKQLKIDVTHDDMVTAEDLVKASKNPKTAYKTLIM
                     SMGTSLKGMGGAGVDIDKEVARCNALADQAKKLGMTIVGIQIEGSARRTDESDEKSNR
                     AVTSKSDILIVRKEVDKDGFFTNAAKQKGIPIIRTVESMDFKYAFSVLFR"
     gene            21676..22962
                     /locus_tag="PLU15_03705"
     CDS             21676..22962
                     /locus_tag="PLU15_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOO30059.1"
                     /translation="MYAHAAMVLAVIAVAFVIAILLRLGTEISMLIAAIAGAIAHGAG
                     IPARHIVEGTFTYFDVVFIFITATFFMNLLKEAGGVTYIVRGIISKFHKNRVALLLLL
                     TLIMLVPGALTGSGTVTVLVVGSFVGTVLSYMGIPKDRVAAAVFMGSAMSAAAPPINL
                     WAMMAAAGANMPYVGFTLPLGIISVAGALFSIFYLARGSKPIDAEQALKEIPEAPEGM
                     AWYKVAIPFVVFFGIIILGRQFPFSMPVIGLPLAFMLSALAVVLLSPVKLRVFEILSN
                     TVEDLLPLISTVTIVGILVQIMALSGARGLISLSVVTMPLAAIFAALFVVLPVSEGIF
                     QYASAPLLGVPLVFLLNMKGYDPVIALAGMASMWPLGDCLPPTAPVGRGAVMAVGYDG
                     DYKAFLKQCLVPALFVLVIGTLFVVFSPKLGFLARG"
     gene            22982..23137
                     /locus_tag="PLU15_03710"
     CDS             22982..23137
                     /locus_tag="PLU15_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30060.1"
                     /translation="MQSVISVLYYVITAMITAVLMFNMFRAKDIKKVILYAAVLIPFA
                     LRVARIK"
     gene            23166..24347
                     /locus_tag="PLU15_03715"
     CDS             23166..24347
                     /locus_tag="PLU15_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOO30061.1"
                     /translation="MSNKKDQNGVNRWAMLIVIVLWLAVVIPGGKEIYGHRHFDEPVV
                     LGPGVTEVKKLSDYSPAVKGTINDCNIYVLDSGEPGGTVFVLGGTHPEEPAGVLSAVT
                     IVENVIPTNGKILVAVRGNYSASTVTRPGDAYPLYYSIKTDFGERTFRMGDRWSNPLD
                     SWPDPEVYINYPSKQLLAYMDVRNLNRTWPGRHNGMITERTCAAFIDLIKQENVDLFF
                     DLHEAELEYSVISTIVAHQNAADIASMASMMLSSSEFHMGMEYSPETLHGLSHREVGD
                     FTDALAFEIEVPEPFLDRVRGVTDEKLLLEGKDEFVMKAGEHGLLYESIDENGWPIDE
                     RVGRTNSTIDMIITMFNEFEPGREIGYVNLPLRDEVVANGLGSYLLDPNSAAPDRVVF
                     D"
     gene            24618..25937
                     /locus_tag="PLU15_03720"
     CDS             24618..25937
                     /locus_tag="PLU15_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO30062.1"
                     /translation="MIEIGRHCNSRFWVPPELVEYDALSGDVNLDRTALPLSSEHIVF
                     RRYGALRRAANALNALPTGSNVLAVVGASSSKAPQVCSMIEGLQSMEEPRVRLLDVRG
                     HADHAIIREGIDIARKTDAALVMAIGGGTTLDVGKAIAGLALQDGGSDIASFQLKKMS
                     INPKQALPWIAVPTTSGTGSESSNNAVVELGEEKRSIRPIPPPFMILADPSLTDSLPF
                     GPTVVSAIDALAQSLEVFTSAGAPMGVQSVALAAFLNLAEGLKALSCATERPESDVRD
                     RLCWGSLLMGIAFAHAHLGLPHALVHHCLRFGLSHGNMVGILLAPGLEIQAAADKETT
                     KRLCKAEQALLALDQDDAFELEAIREGMASNERCTVPDRLIEWLEDLIDQLFTAADLP
                     TSLGSAGLTRCDLDWIASNELESGASFGIPNRPATYGEIMEVLERAF"
     gene            complement(25981..26055)
                     /locus_tag="PLU15_03725"
     tRNA            complement(25981..26055)
                     /locus_tag="PLU15_03725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26021..26023),aa:Gly,seq:ccc)
     gene            complement(26449..27348)
                     /gene="sdaAA"
                     /locus_tag="PLU15_03730"
     CDS             complement(26449..27348)
                     /gene="sdaAA"
                     /locus_tag="PLU15_03730"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO30063.1"
                     /translation="MAITTLAELVEEASSRGVSIGAIVREREAEQSGRPEDDLDADMR
                     ERLGVMRSAVDRGLAGVRSRSGLTGGDGPRMASARMNGETICGEPFDSALAYALAVAE
                     VNASMGRIVAAPTAGSCGVLPGVLLSVGERLCVDDLALVEALFAAGGVGAVIERSSTL
                     SGAAAGCQAECGSAAAMASAAAVQLAGGSPEACASAVAIAFKGLLGLVCDPVAGLVEV
                     PCIKRNATSVAVALAAADMALAGIRSVIPADEVIEAMGSIGRALPPSLRETALGGLAV
                     TPTGKKVASGISVLGHGNCGLDV"
     gene            complement(27361..28035)
                     /gene="sdaAB"
                     /locus_tag="PLU15_03735"
     CDS             complement(27361..28035)
                     /gene="sdaAB"
                     /locus_tag="PLU15_03735"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOO30064.1"
                     /translation="MRTVFNIIGPVMVGPSSSHTAGAVRIGLAARAIIGGQPSRAVIT
                     LYGSFATTYGGHGTDLALIAGLLGMSPDDERIPDARRLAAQSGLDVKFEVAEASPDIH
                     PNTAKLELVGPFGATTSVVGSSIGGGSILITKIDHFEVDFSGESHAIVVAYRDQPGII
                     AKITSLLATEDVNIAAMRVSREAKHAKALAIIEVDQPVSDEARALISRIVGVDTAISL
                     PPVSLA"
     gene            28273..28992
                     /locus_tag="PLU15_03740"
     CDS             28273..28992
                     /locus_tag="PLU15_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOO30065.1"
                     /translation="MGNSREGILNGFDLKLIAAALMVLDHVLEYFPSAPIWFGYLGRI
                     VAPIFFYLTVEGFFHTRSRVNYMARLFLGAAVMYVGSRILTHFMPTPVGIPNNIFVSL
                     GLGVALMSAFDWTRETGKYWTGIPMIIAIAAVSLSTEASLYGVAMISVFYFCRNNTKM
                     MAIMYIATSLMISWPSPALNKTHILLYDYQWMMVFATPFFFLYNGKRGPGGEAAKWFF
                     YIFYPAHLWIIYVIQYFTFWV"
     gene            complement(29138..29734)
                     /locus_tag="PLU15_03745"
     CDS             complement(29138..29734)
                     /locus_tag="PLU15_03745"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO30066.1"
                     /translation="MGQNSRAQYRRIATMGVLTALSIVMLLVLRIPFPPAPFLIYEPA
                     DVALLVAGFALGPVSGLVCTAVACVFQALLLSSDGWFGGLMHLISSGALVAVSAMIYS
                     RNKTKRGAYLALLAGGVAMVAAMIPANIFLIPMFYGMPRQVVVDMIGWIVLFNVLKAG
                     INSVLTALVYKPISGLIKAVPSGSGRSTVKAASKETWR"
     regulatory      complement(29819..29980)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            30063..30183
                     /locus_tag="PLU15_03750"
                     /pseudo
     CDS             30063..30183
                     /locus_tag="PLU15_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630198.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            30397..31419
                     /locus_tag="PLU15_03755"
     CDS             30397..31419
                     /locus_tag="PLU15_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOO30067.1"
                     /translation="MSGDEKQRPRKRWPWIVAILVIAVITLIVSSGKHDRDGVSTRLA
                     AAVAGQETIAVSTGDVHEFVSAAGHLMPIATKTLSFDMPGRITRLKVEEGSRVTAGSV
                     VCELEANSSASLPGDYLRSLQEWVPTPDTGAAEKKLASIRAPFDGVAVSVNASVGSYV
                     TAGQPVITVIDTSSHVVDIEIDEIDLTRVSPGMEATITFDAIPGLKLPGKVDSIAQMA
                     VPKGGIVTIPVRISVDGWHELLKPGLTASVKILSRTLSDVVKIPIRSWIDHEGEPSAI
                     RIEDDIPIFVPLELGLSDGEYTHVLSGLEPGDIIVLDAVAVQEKLRRAAGQDRFITFD
                     VQRIDD"
     gene            31438..32142
                     /locus_tag="PLU15_03760"
     CDS             31438..32142
                     /locus_tag="PLU15_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30068.1"
                     /translation="MVDLENVTKIYSMGSFEVTAIQDITLSIADADFVSIMGPSGSGK
                     STLMHVLGCLDRPTSGRYRLCGQDVSRLDDASLAQIRNKRIGFVFQQFNLLPRETILS
                     NVEVPLIYAGVKAKERRRIATEALERVGLSDRMRHRPSEISGGQKQRAAIARALVNNP
                     SLILADEPTGNLDSKTGHEILDILSQLNSEGATVVLVTHDREIAERANRIVHLRDGAI
                     ELVEELRKGGGADGVS"
     gene            32129..33331
                     /locus_tag="PLU15_03765"
     CDS             32129..33331
                     /locus_tag="PLU15_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30069.1"
                     /translation="MGFLETIGLAFRNLWANRLRTMLSALGIVIGVASVITMISVGTS
                     AERRIASEIAGLGSNMILVTPKIGRGAKSASSPSLTIEMAEELKAAAPAVSAVSPVAQ
                     IEGQLRWASSSTNTSIIGITSDYMRIANQSIVRGRSLIPAEVDSAAKSIILGAKTASA
                     LFEDEDPIGREIGVVAAGRRFDFTVVGVVAAKSDMLFMNSDSQAFVPITCLLYRMMPI
                     RGISMYYCQAQSPEMAGPAVAQVERYLASRTGNPGAFNVGSQEALLDAVHQVTSTMTL
                     LLGIIAGISLLVGGIGIMNTLLTSVAERTREIGIRKSLGARNRDILLQFLVEALALSL
                     IGGAIGMILGWMAGSGISRLAGWPPSFSMTSVAAAVGFSSAVGLIFGIFPAVKAAQMN
                     PVDALRYE"
     gene            33359..>33627
                     /locus_tag="PLU15_03770"
     CDS             33359..>33627
                     /locus_tag="PLU15_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30070.1"
                     /translation="MGPKCNMASSIYGRVGGVIDGLSMNCATDSGSIHGRIGGDIFGA
                     DIDLAVDELRGTVSGRIGGVIIGRSVDAKYHAGSNASLYGRVGGI"
     assembly_gap    33628..33727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33789..35006)
                     /locus_tag="PLU15_03775"
     CDS             complement(33789..35006)
                     /locus_tag="PLU15_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOO30071.1"
                     /translation="MKLRRTAAVLAAILVTSLLVAGCGPISSTTTGSIAGCVVKHSMA
                     RSLSSPELQDKAVIIVPEPPADSSYVGLPGVRVVAESTGYPSRTYIATTNRYGDFRMS
                     QVEPGEYVVKIADDRFILGYAIQCRVQAGKPTYVGTVPMGSLHLLSVGVSTYRNPSLA
                     PNLTYAASDAGLIAQVLGVDNKLVARTVRLCDYDATKAGILGAIASMGREMVPSDTFI
                     MFFSGHGYQNASTNTEYIVPHDYDGYLTSLIADYELNDYLDRYIPAIHKVFVFDSCYS
                     GGMFKVLDQSMPAGFTRSTGFEIMAKNIVGPGKIVITACDKNEQSIEGPQWNGGLFTW
                     LFAGGMEPYGQVPYPADGCFNHVRDGIITTQEIFDYVEYYVPKEAERVSHTQNPVIYR
                     GPDGNGVWPLFTY"
     gene            35213..36460
                     /locus_tag="PLU15_03780"
     CDS             35213..36460
                     /locus_tag="PLU15_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30072.1"
                     /translation="MSRSRLLTLLCVALICATLIGHGPNTAVAMPSHQIASRGQTLVE
                     RESDSQSILTLFPELKSLTAPLWVQEGLRAYYRTSAATVPNPLSGPNKDAVKPPIMHP
                     EELSPAAPALVRTDVIALATRFSATCSTLFIGDLMIPWEDVTRAAYAGAGDFWINPIV
                     FKDIAKRAPEHLSISEIQFEAGGTKYDSIRFDYRPTGSNKHYVWIFQSSTGLLIYQAQ
                     WLTLSDQRLAYVSAAEFVTKRNISLPWSGEAAPPWAKPGAKLTYQGTIRTWLPTTGHN
                     IPIPALLEVKIEDGGRNWTVAKVTRVFSDDRGQTGAAIKVYSGTGQIGGFWLPKAGLA
                     KLKNGNKLDPFDPLVGSSVEVSYVGKTPSGISVAAIFEWGAKYKRVWIYRSTDGMLLY
                     WLDEKVVDPVTGMVQQAEWQLME"
     gene            complement(36450..37982)
                     /locus_tag="PLU15_03785"
     CDS             complement(36450..37982)
                     /locus_tag="PLU15_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOO30073.1"
                     /translation="MMKMVGARRRVLTLLIAGLVAALGLSPGAGGAPKQEEAATIQTF
                     AGYGDLLTLSSPPVSGISVPGTIQVSGRSCCSTVLIRCSRQDDPAKFANTFVTVGDDG
                     GFSATVWFFDGPGRYVMDIAAVPRGETVFRVTCRLVVDNTLSTIPLAPRQYSGYGSDL
                     EINSPIASSNRVSDFVTITGKSAFPAVWTSIENAKTGQRRDHIAETLPNGDFAITIPL
                     DVGEGECRISVASRLIAPGTYNTVAEYLVVVDPAPIDMIEPAYRGGTILACDRFLIKG
                     VANADGVRVDVERDGKSAASLKRSRAGAFGLWIDPPAIGEECLVTISSLHRDTNRCVP
                     RRKYRVVRVPKIPLLDDSEKRQIQWDDAEIRVLAESIAPDARDPYDRLRAIHDWIASN
                     LAYDVRAAASGDFGPTDAISVIARGGSVCQGYANVTAALAAAAGLRVRVVGGEGIQEG
                     IRESHAWNEAEVNGRIVLLDTTWDAGHVKDGRFVRMPAAIWFDSSPEEFSLSHSGGLI
                     IP"
     gene            complement(37985..38701)
                     /locus_tag="PLU15_03790"
     CDS             complement(37985..38701)
                     /locus_tag="PLU15_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOO30074.1"
                     /translation="MEDANWTERTELIIGADGVARLAGSSVAVVGLGGVGSACAEALA
                     RAHVGKLIFVDGDVVSTTNINRQIIAFTDNIGRPKAEIMVERVRRINPQCEAVAEVCW
                     VDGSNVGDLLRLRPDFVADAIDSVGAKLDLIEACLRSQIPIVSSMGAGNRLDPTAFAI
                     ADISKTHTCPMARSVRQGLRRRGIETGLTVVFSDEPPVVKSSGPIGSISTVPPAAGLA
                     MASVIIRQLASDSGGYGVFQ"
     gene            38900..39781
                     /locus_tag="PLU15_03795"
     CDS             38900..39781
                     /locus_tag="PLU15_03795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOO30075.1"
                     /translation="MAQDIASGSVDVVVTSPPYNLGIEYGTYDDRIDRDEYLEWTRAW
                     TSEVARVLSPKGSFFLNIGSKPSDPWVPFEVAMELRETFVLQNVIHWVKSIAISHEDV
                     GDYPAIREDIAVGHYKPINSKRFINDCHEYIFHFTHNGDVTLDRLAVGVPYQDKSNIN
                     RWTREGSSPIDLRCRGNTWFIPYETITRRSSERPHPATFPPKLPEMCIKLHGLDRCRL
                     VIDPFLGLGNTAIACVRLGMPFVGYEIDRFYFAKSVERVTQAVKEREIIEDDSRVQVT
                     QQSLFPEHDQTEKNASL"
     gene            complement(39771..40718)
                     /locus_tag="PLU15_03800"
     CDS             complement(39771..40718)
                     /locus_tag="PLU15_03800"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOO30076.1"
                     /translation="MFIMDAHCDTLMDIVEGRRRLADRDKGGQLDLARMMEAGVSAQV
                     FAIYVPPQYLPGGGARRALQYVDAFYREIEENSDKLVFATSGRDVEEAYEEGKIAALL
                     SIEGGEALDGCIELLRIFYRLGVRLMTLTWSLRNELADGAFESRTGGGLTRFGVEVLE
                     EMDRLGMVVDVSHLSEAGFWDVMEVSKRPVIASHSNSKTLCDHPRNLTDDQARAIAQK
                     GGVIGVNFAGYFLGEERRSLAGVVDHIDHFASLIGPECIALGSDFDGLPDEQLPDELK
                     DVSSLGKVAEELAARGYSESAIQGIMGENLLRAVRAVIG"
     gene            40868..>41712
                     /locus_tag="PLU15_03805"
     CDS             40868..>41712
                     /locus_tag="PLU15_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO30077.1"
                     /translation="MPKIKIVTDSTADLGPELIKRYGVEVVPLIVTFDEETYTDGVDI
                     DTHRLFQLVKEKGKLPKTSSPSPETFRSVFEKAIADGSQVIYIGISSQFSASVQSARL
                     VANTFPEGQVHVFDSANLSTGIAQLVLYAHDLAAENKTVPEIMDLLEKARPKVKTCFM
                     IDTLEYMHMGGRCTGIQTLLTSVLKVRPIIEVVDGGMRVAAKIRGSRTKLLDWQLTRF
                     AQDVERGLVEPVRVFLTHTGVHEDALYMADRIRRIMPEMREILETPAGSVIGSHCGPG
                     TIGIL"
BASE COUNT         9701 a        12060 c        10577 g         9174 t
ORIGIN      
        1 ataagggatg tctcggcgtc ccttttgagc catggttgcc cggccttcgt tagagcagca
       61 gactttttgg gagatgcttg gccgccatcc gatcaacatg tctcgcagcg gaaagccctg
      121 ctacaagacc ctctcccaca gccttcgcaa tctggtaagg cctacccgta caatcgcctg
      181 ccgcatacac cccaggcaca ttcgtagcca tgtcgcggtc aaccactatc gcgccatcag
      241 cgacgcggac gccttccaag agcctttcca ctggaatcga atccctgatc acaaagacac
      301 cgtctgcagg aatagcctcg ccgtctagtt cgacttcttc caccttgtcc tcgccgcgga
      361 tgccgagcac tctcttcttc acaacgcgaa tgccttcggc aatagccggc ccgtctccag
      421 gttccacgct gcgcgccatc acatatgtaa cctcagagca gatctgggca agaaagttgg
      481 cttcctctat agcatgctca gtatagccaa gaacaacgac cttcttccca cgataaaggg
      541 gtccatcgca tgttgcgcag tagctcacgc ccattcctaa aagctccgat tccccgggaa
      601 tagatgcctc ctggactgag cctgtagcta tcacgaccgc cttggcgcta aactcgccgt
      661 cagaacctaa agccacaaag gaatcaccga ggttgaacac gcccgtgatg tccgattcga
      721 tcagctcggc cccggcagcg cgcgcatgat tcacgaaacg agcggaaagc tcgcgcccgg
      781 agattcgctc taccccaagg tagttttcga tccaaggcgc cttctcgaga ctggggctcg
      841 tgagccttcg cgatattacc gccaccgact tcttacgcac acaggcattg accgcagcag
      901 aaagccctgc agggcccgct ccgatgatca ttgcatcgta ttctccggaa gacaccgtct
      961 gatcagctcc ttgcagcaaa cgcggcctcc acacagtttt tcatcagcat cgctatggtc
     1021 atcgggccca caccgcccgg cacaggagtg atagctccgg ccacttcaac cgcctcatca
     1081 aaatcgacat cgcccacgag cttctttccg ccaaccctgt tggttccgac atctatgacc
     1141 acagcgcccg gtttgatcca gtctccctta accatcttcg gacgtcctac tgccaccacc
     1201 agaacatcag cttccctaca tacagaaggc aagtcctcag ttcgggaatg acaaatcgta
     1261 acggtggcgt gttcggcaag gagcattatc gaaaccggct tgcccactat gttgctcctt
     1321 ccaacaacca cagcgcgctt gcccttcaat tctatgccgg tgcgatggat aagctccatt
     1381 atccccgcag gagtgcacgg gcgaagcgca ggcttgccca ccatcaggct gcccacattg
     1441 atcggatgga agccgtcgac atcctttgag ggggaaatcg cttcaatgac cttgtcctcg
     1501 gaaatgtggt ttggaagagg aagctgcact agcaccccgt caatcttcgg atcagcattt
     1561 aaccttctaa ccaaatcgag caactcgtcc tctgtagttg ttgccggcaa ggcatgttcc
     1621 tcagagtaga tgtcacagcc tgcacaggct ttcttcttgt tgttcacata aacacgggat
     1681 gctgggtcat ctccaacgat cacgactgca agccccggag cgcgtccgaa cttctcacga
     1741 tattcagcag ccctcgcagc ggcatcctcc ctaatcgatg cggcaacggc tttgccgtct
     1801 atgatggttg caggcatcag atcaactccc cattttcttt ctgcaacttg caagttgcca
     1861 taatgatccc ctatccccca cacgcatggg ggggaatata gcggtgttat gccagtacgg
     1921 ccaggattcc ctaaagccat aactggcaaa caaccattcg cttggattat attataccat
     1981 ggactcaacg gcagatgtga gcacgctgtc ctcgctgaca aaacagcgct tcccgttagg
     2041 gagtttgacg cgtagaagaa acggcgggcc gaacacaagt ccctccacac acttgcgttc
     2101 ggactccaaa attgcaacgt cggtgccggg atcaatcctg aggccatcgt cggcgccggg
     2161 gtattccatg ccaatggccc cttcccttcc gatagtaccc tctccaagaa cgaacgtttt
     2221 ccatctgtcg ctccaacaga atgtttgagt gaccttgcgg gagcttatga gcttgaacgc
     2281 gtcatctttc tcactgtagg aatggtattc gtgaagcgac tccataacaa ccgaggggac
     2341 cgggccaccc gaaaactgca cagtggaaga gctagccaga cgaacccaat tgccggggcc
     2401 aaggtcgaac cctttcgccg ccgtggcagg aatccccccc gaactggtaa agtcgcgaag
     2461 cgccctctcc cttgccacgc cccatcgcca ggagttggcc ttcacatttc cgaccgctcc
     2521 ccaggacaag ttccatacgg cctcggagaa ggtggtcgcc gaccacactt ccaccaacgt
     2581 ccgcccattc caggcatagg cagttgatac cgttgtcttg ctcattgcgc ccacactctg
     2641 gtcatagttg gcctggacaa ggatgagagt cgcacacatg gaatcactag tcgttcgctc
     2701 ggactcagac gcgcgctctg gcttcgaatc ggatgtccta tggctctctt gactcggaga
     2761 cgattcgtaa ccacagtcca acacgatctt ctcaagtcca actacggggc cggcatccac
     2821 ccgcgccaga agcttgtagc cgccctggcc tcctgttccc gccttgccat ggagttccag
     2881 atagccttcc accggcgagg tccaatggga tgtggccacc tcctcccctg gatcgttggt
     2941 tatgtcaata tcaaggctga gaacaggtcc taccggcgct gacggactgc ttcctgttgg
     3001 tagaaaccag ctgtttcctg tttgagtcgg ctccgctatg ctccctaggg agaggacgag
     3061 accgcatatg aagaacagaa tagccgccat cagaaccctg tgaacgctta tgtccggcaa
     3121 ggattggaaa gacaatcgac gtgggtggcg caaagatgct agcccgggcc gtgaacctga
     3181 tgcatcaggc tgtcgaatgc gagtatatcc ttctcggcgc atgtgatacg ccccctacac
     3241 gataagactg agtaaatcct actcctgtcg ggggcgaatg attcatcgat gattgcttcg
     3301 gaaacattga cgatgtcagg cgccgtcatg cccaacctgc aggctttcga cgccgaccgg
     3361 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntattactgc ctgcggcacc
     3481 gcattcttgc aacaaaggcg cgctgctcag cactgcctta tgatggacat gacccaactg
     3541 catacatcat cggccaactc gaccgcgcgc aagtattcct cctcacttac agcctcataa
     3601 ttccccggat acctagttgt taccgcatag tctgacaaga ctgccgcttc ttgcacgtgt
     3661 ggaggaattg ccatcttgct gccatcttcg atcagcttca gcagataacc gatggaatgt
     3721 gtacgcggag catccatccc cagatagagg agaagcgcct ttaggctttt ttctgcagcc
     3781 tgctgtgcat caaaagcaag atcctcgtac aacacatcct ctgagaattt cccgtgcttt
     3841 gcgcgcgcaa gattgcttct agctctgcag atccaaattg accacactct atcgttcata
     3901 aagaacacgc cctctcagag cttcgccata tatgagtccc ggaacatcgc gcaactgctc
     3961 tgccttgtcg gggtcttcca caaccaagtc gataggagca ggaatcactg ctagctcgcg
     4021 ataaagctgc tgcgcaagtg tccgcttatg gtaatcaccc ttttttagga ccaatatgtc
     4081 atagtcactg tcagggcttt gatcaccact ggccctggat ccaaacaaaa tgatcttatc
     4141 gggatctgca gttgatacaa ttatctttac tatcgccgaa aggacatcca cagtgtttca
     4201 cctccgcaca atccacgtta agtggccggt gcttttgcgg ggtatggtcc gattcgacaa
     4261 ggcatgactt ccccacccga aagggaatgt cctccacaat cgcgtgcccc atctggcaag
     4321 aggcccggaa tatgctggcg atgattttat cgtggaaacg tacagcatac gctcagtttt
     4381 gcacgatccc ttggtccaat tgagcagcac aagctcagaa catgagcaat gcaaactgaa
     4441 aactcgcgcg gcaacttatc acaaaacttg ctcagatgct cggcctcgct ccggccggat
     4501 aatacagctc tctgcctacc cggcctcgca ctcatggcgc ttgccaagat tccgccccaa
     4561 tctcagcgct tcctgccgac gtcattcctt gcatccggca acttccacgt atctcccgac
     4621 aatctccttg aatgttcgac ctctctcctc aaagtttcgg aacatgccat agctagcaca
     4681 ggccggcgag agcagaacaa catctccagg cgaagctgat gcgcgcgctg cgtccactgc
     4741 ctcttcgaac accctggccc tgacgatcct cggcggctcc gactctgccc tgcgtgcggc
     4801 ctccacaaga gccttctcta tcttgtcgga ggtgactcca ataaggacca cagtatgaac
     4861 cttcccgagc gccacctctg ccatctcgtc aaaaggcagg ttcttatcgt agcctccagc
     4921 gatgagcact atgggtcgct ccaccgcatt cagtgcggca attgttcgcg tcggcgtggt
     4981 ggcaatggag tcgtttatat aggcaacacc acctacctcg cccaccgcct caagcctgtg
     5041 ctcgactccc tcgaaagagc tgacagcgtg tcgcatcgct gatggatcgg cgcctacagc
     5101 gctgcataaa gcgatggcag ccagcacatt cctgacattg tgcatgccag gaatcttgat
     5161 atcggacctt tccatgacta ttgtatcttt gcagacccca tcggcaacgc atcgcaatac
     5221 caatgactcg ccatccagaa aagcccctct gtccaccggc ccgctcgcgt cgaacatcag
     5281 cacattgcca cgcgctctag tcgccatgcg cgcagttatt tcgttttcct ctccgaatac
     5341 ggcccaatca tcctcagatt ggaaacggac gatattctcc ttcgcgaaga tgtagtcctc
     5401 catgttcgga tggacgtcga ggtgatttgg ggttatgttg agcaccgcag ctatatgcgg
     5461 gctgacggcc aagagcctca actgaaagct ggaaagctcc agaacaacga ggtcctcgct
     5521 ttccactgat aatacgtgct ctattagtgg ggtgccaata ttgccgccta cgagcacgtc
     5581 cctttcgggg tggtggaggc gtgcatcttc cgccaatatc cttcctgtca aggtagtagt
     5641 cgttgtcttg cctgaactcc cggtaatgcc gatcaccggc gctgcagaaa gcttcaatac
     5701 gagctcgatc tcggagctga atgagactcc gacatcgcgc gcagccatga tctgcggttg
     5761 ttccggattt actcccggag agaggaagac aatgtcgcaa tcggccagag aatcgagata
     5821 tcccggtccg agcgagacat ctattcccag tcccgaaagg gacgccaact ccgaccccag
     5881 ttgatctgct gtttttatat cgcaggcagt aaccttggca cctgcttggg caagaaagcg
     5941 tatgagtggg acgtttgtaa tcccgatccc cactactgtg acccgctttc gggcaagcgt
     6001 tcccataaca cgcttctgat tatctgtcaa catcatgaag gccccccaac atcaagtccc
     6061 aaccccccgg catcttccta gactgtatct tgttgttctg cagtcgtgcc catgccgagg
     6121 atcatttacg gaaataagtg cgctttggtc atcccctctg gcgcctccct atttcgataa
     6181 gcctggccaa tgtttcagct gcatcagcca cgttggcata agcgcgcctg accgctgcgt
     6241 ccaggtcgca cggccctttg accacggtaa gcagggcatc gattccgtga tcatgtagtc
     6301 ctctggcgtc gtcagccaca gacccggcca ataccgccac cggaacgccg cactccgttg
     6361 cgaccctggc aacccccata ggggtcttgc cataaagcgt ttggaaatcg atacggccct
     6421 ctcccgtaat gaccagatct gcacctgata ccttctcttt aagtcccgtg gtctcaatta
     6481 cgatttcgac cccgggctca agacgagcac cgaggaaggc cacaaggcct ccgcccaatc
     6541 ctcctgctgc ccccgcccca ggcatcgcct ctacgtcgat tctcaggtcg cgccggatga
     6601 cagaggcata gtttgcgaga gcagcgttga ggacttgaac catctccggg gtggcgccct
     6661 tttgcggacc gtataccgcc gacgctccat taggccctgt aagcgggttg tccacgtcgc
     6721 ttgccacaac gaattcggct ccaaccagcc gtcgatccat accggacgtg tctatcctgg
     6781 tgagcctcgc caactccgcg cctccaggtc ctatctcttt tccttcagcg tccagcaatc
     6841 gcgcccccaa cgcctgggcc atccctgccc cgccatcatt ggtggcgctg cctcctatcc
     6901 ctatgatgaa cttccggcag cccctgtcca gcgccgcggc gatgagttct cctgttcccc
     6961 gcgtcgtagc tgtcatagga ttccgcttgt ctaaaggcac aagcggtagg cccgacgcgg
     7021 ctgccatctc gatcacagct gtcgttccat cgcctaggat gccgaagaaa gcctgcacct
     7081 tgtcgcccaa aggccctgtg gcttcaacat caataacttg accgccggta gcatcaacta
     7141 acgcctcaac ggtcccctct ccaccgtcgg ccatagggac acgcaccact tcagcgtcgg
     7201 gccacaccct gcgaagccct tcttctgcag ctcggcacac ttcggttgca gcaagacttc
     7261 ccttgaacga atccggagct atgactacct tcaacgatct ggccaaacac aatcccccca
     7321 aaaaacctta tcagcacaat ccctggcgag ctccatctta tgggcacaca aataccatta
     7381 gtcatcaacc cagcgcatct actgcgcatc tcaggtgtgc cccctacgcc aattcctgct
     7441 ccatctcttc cgctatacct gttgccattt caagcaagca ttcctttttg ttggccgacg
     7501 gatcgtggag cacttggtca agcagccgct gcaaaacgat cctgactctg ggtccttggc
     7561 tgaggcctgt tgcctccata acctccttgc cactgatgtc gaggtcagca attctcagcg
     7621 caggctttgc ggccagcact gacccggctc gttcctccaa gctttcaact gcctcgattc
     7681 cctcactccc gagatccgca accgccaaag accctgtccc ctgcctgcac aatgcgcggt
     7741 tgagccgaac agccggaagc aggcaatcct gcccaactcg ggacgccgtc agtctcagtt
     7801 cgtagtctga tgggttttgg ccaaggtttt gagcgccttc aattattcga gacaccgtaa
     7861 tggaaaactg gttggagacc cgcattctcg ccaaggcaat gcgagcaccg ttcggcccca
     7921 atggcgaaag gagagccgcg aagcgctcct taggctctgg atgcaatcta tcacaggctg
     7981 cataaagggc cttcctttct tcttctccga cctcgcaaca caactcagga aggatatagg
     8041 gggcaagtcc catatcctcc atgagtacga gagctgcacc gggtttcgga gcacgcacgg
     8101 ttttcacaag ctcatccata atgcgttcca ccgatacctg tacgatccct ggcgccaacg
     8161 cccgcgcggc atccacaact gaagagtcta cactgaatcc gagcgttgcc gcaaaacgta
     8221 cagccctgag catcctaagc ttgtcttcgc tgaaccgttc agcaggatcg cccacacagc
     8281 ggatgagtct ggcctcaatg tcggacacgc cgccataggg atcgactagc tcgcctgtgg
     8341 tcggatcgaa tgccattgca tttattgtaa aatcccgcct tgcaagatcg gcagcgatgt
     8401 catgcaaaaa caccacatgc tccgggcgcc tgccatcaac atacgggccg tcatgtctgt
     8461 acgtggtcac ctctatgctc atgccttcct ctattactgt gacagttcca tgagctaacc
     8521 ccgtgggcac gacatgctca aaacacgaac ttacctcggc cggagtagca gaagtagcga
     8581 tgtcccaatc gacagggcgg cgctggtcga taatcatgtc ccgcacagct ccccctgcaa
     8641 tgtacgcggt atgtccacag ctttttagct tctccacaac aatcacaaca ggctgtggcg
     8701 gagcaattcc tgctttccga tgcatccaaa cacgcactcc ttccgcattc tagtgtacgc
     8761 acgcaggcaa attccagcaa gtgcccattg aggatcctgt gtccctcaca taccggcgta
     8821 gtttcaggta taattgacac agggaactcg cctcttgcgt cagtcgttat tacgggtaga
     8881 cgctcaaatg gcatgtgagt gggtgattca attgagcaac gaatatgcga ttgcaagacc
     8941 tgcacagccc gtgtcgaagg ccacggagtt cgaatctctc gttcggaggc accaaaactc
     9001 cgtttacaat attgcacttc gaatgagcgg cgaccctgag gaggcccgtg atttgacaca
     9061 ggaggctttc gtcagggctt acagatcgtt cgacagctat aagcggggaa cctccttcga
     9121 caagtggctg tatcgcatag tgactaatct atatatagac gaactgcggc gtcgaaaacg
     9181 gagaccgtat atcgagtctc ttgaccaacg aattactaca tccgagggag atgaactcga
     9241 caaggagatc ccggatatgt ccagcaatcc agagacagtc tatgacaggg accatcttga
     9301 tgcattcatt caagacgcgc tcgattccat ttccccagag tatcggatgg ctgtcattct
     9361 ctgcgacatt gaaggcttct cctatgaaga gatagcgcag ataatgggca catccattgg
     9421 gaccgtaaga tcacggatac accggggcag gcgagcgctc cgggagcgaa tctctcctta
     9481 cctcgaagag cggtcgcggg aggtgagcca aaagtgacgt gcaacatggc tgagcgcctc
     9541 atttctgcct atatcgacca tgaactctct gaagatgaca ggaacagggt caggcagcat
     9601 ctggccgtct gttcaagctg catggcaaca tacacagcaa ccctggaggt gaagcgcgcg
     9661 ctaagctcga tgacccctgc gcaatgtccg gaatgtgtat gggaaaaggt ggaacgccag
     9721 ataaccttga ttccttttga tagggagatg aagcgggacg attctacgaa acctgcctgg
     9781 tcattcatta agttggtgat tccggcggcg gtcgctggga cgctcattgt tatcccattg
     9841 gttcaggcgc tccttggaat cagcttgttc ggcgcaccag acaaatcgca accgccgatt
     9901 gcatccagca agcctaatcc ccagttggaa ctgacatctc ccagtgttcg tatccatgca
     9961 gccggaacat tcagttcaag cgatgctctg gaggaagtcc ttggggaaaa catttggctg
    10021 ctcgagccca cgcaggcttc aggaaaagcg gcaaacgcca gccaacatgg tctggatctt
    10081 cgtgatatct acgatgacta ttttatgcaa gtaacatctc cagaacggag gtatggtaga
    10141 tgaaagcatc ttttaagaag tatctgcccc tggtcgttat cgcgataata agcagcgtga
    10201 taacgtcgat tgtagtggtg tcatacgggt tccctgaacg caaaggagac actgccgcag
    10261 cggtaacgcc gatcgttgcc tcaagctaca gcggaaacct gccatctata gcagatgtag
    10321 cagaaacggt agggccagct gtggtcagaa tcgacactga gcgagtggta agctacaggt
    10381 cccctttccc cgatgacggc ttcttcggtc cgtttttccg agatttcttc ggcggcgaga
    10441 gaatacagcc gggcacgggc tcagggttca tcatcagtcc agacgggtat atcgttacca
    10501 actatcacgt ggtagaagag tcccggaaag tggaggttac actgaccgat ggacgaaagt
    10561 tcactgcaag agtcgttaac ggaaacaaag atgccgatgt ggcggtcatc aagataaagg
    10621 ccaataacct ccccgtagcc cagctgggcg actcgagtaa gttgcgtcaa ggggattggg
    10681 tagtggctat cggaaatccc tacgggtttg atcactctgt tactgcaggg gttgtgagcg
    10741 cggtcggaag acgcatagac gacgagagtg gcggaactct ggccacgggg gatctcatcc
    10801 aaactgacgc ggccatcaat agcggaaaca gcggaggtcc gctcatcaac atggaaggca
    10861 aagtgatcgg cataaacacg gcgatcatcc cgtacgcaca gggacttggg tttgccatat
    10921 ccatcaattc cgtcaaagat acgctgaaag acatcgtcaa ttacgatgaa gggcccaccc
    10981 catggattgg aatctggtat cagcagctcg atgacgatat cgcaaagcag ctgggcctgt
    11041 cggatgccaa cggaatcctt gtgaccaatg tgatacaaga tagtccggca gaaaaagccg
    11101 gactcaaaga aggagatgtc atcaaggaga tcgacgggaa acagattacc gcaaagatgt
    11161 cgctcgccga ggagataagc aagaagaagg tgggcgacaa ggtagtgatg tatgttctga
    11221 ggggagggca gaagcactac ctcaccgtag tgcttggcga gtatcccgga gacacgctca
    11281 tgctaaagta atgctctgtc cagagtgttg aggcagccag acgttgtgcc tcggggaatc
    11341 gctaatggtt gtggcatatg gtcatatttc cccagaatct ttcgaagcca aacttgaata
    11401 agttccattt atgggagcag actatgagcg gcgggcagga taagacgtga catatgggtt
    11461 gtttctcacg gaaacgattt tctcacgcac ctgctcgctc cagattaagt catcgccatg
    11521 tccacccgtc gaaaagaagc cggccgggca gctgcagtct caaccatggc agcgtcagcc
    11581 cggtcggcat ttttgtctcg gacagttctt cggccttcac gacctggcaa acttcttgat
    11641 cacgcgcaca agctcatcga tgacctcgcc ttctccgcct tcggccatcg cctccataac
    11701 gcaatcccgc gcatgcctct cgagtaacag aacgcctgca ttgtacagag cagcgcgcgc
    11761 ggcagcgatt tggtccagtg tgtcagcaca gcatttgtcc tcttctatca tgcgttgtac
    11821 gccttttacc tggccctcta tccgtttaag ccggttaatg acgtcacgct tctgctcatc
    11881 gttcactcca gcaatcctcc tattagggta ttgttttcgc tgataggatc agctcaggcc
    11941 cgtgtctgaa tgtctgagcg atcaggcagt atttctcagt catctccagg atgctggcga
    12001 attgagcttc attaatgcag gcgcccgggg cggacacctc cagccaaatg gaccgcacaa
    12061 atgtgggacg ctcgtcagag atggccgcta ttccctcgat cacgatcccc tgatgctcta
    12121 ttttcatctt gtccatgacc ccgcgcatag tcagtcccga gcaccccact aaactggcaa
    12181 caagatgcgc aacaggactg agcgcgccct caaggcgctg gccatcttcg tcctccagat
    12241 gccagtcctc ttctggctca aacacgccga taactttgat tctgcctccc atcaatcaat
    12301 gccgcctttc aatgtcgaaa gtgtgaaact cctcatgcga gttcacgtcc ggctcatgca
    12361 tttgctcagc ccacacagta cgccacattg cgaaagatga gttctctgcc aggctgcgcc
    12421 ttagaacctc acacgcggcc cgatcgggaa tctatatagt gttttctgcc atttctgaaa
    12481 aacgtccgca atctccccct gttttcgaga tctctcaagg ccctgtctat ttcgctctcc
    12541 ggtgtccttc tcacaaaacg ccttacgatc gccgactttg tcttaggtga tgaacagaac
    12601 tgtatgatgg aatcgtagag tcccgtagca cctgacggca accgcctctt agggtagacg
    12661 actgtatacc cgggatttcc atcaacatct tcacctgcaa cgtagtcaac gcacgcaggg
    12721 tttgcagaca gaagcgctct tatgcaacgg cgggtatctc ctccgacccc tcctgatccg
    12781 tacccatgaa tcacctttag actggatgaa cccgactcaa gggcattatc gtattctctc
    12841 ataaaaaatg acatggcatc tgcggtcttc atgccgtgca ggtccagttc gcgcaaggat
    12901 attcgcctcc aaaacatctg ctaacgccga tcatccgggg catggtgcct cacggctaac
    12961 acagacgttc agcccgccac ccaattgcgg acgcgcgccc tcctgcaaaa caaagggcag
    13021 aaggacgcgc actctactgc tgcatcacgt gcttgcactg cggacaacgt cgactccacg
    13081 cagagtgtcg gagatgggac accttttctc gatcgtctca accagcctgt cgatattctc
    13141 ttggggtgat ggagagtcga tgatcatcct ataccgtatc ccctgatatc cggtgcgcac
    13201 attggggttc ttaccgagaa atccgtctgg atccaggtct ccttcgacct ctacagaaaa
    13261 gccctgcagg tcaactccgc acgccccggc aaaggcagca gcgcatatcg acatgcaccc
    13321 acctaacgcg catagaagga gttccaccgg attcatggcc cggtcagttc cgccgagcgc
    13381 cttcggctcg tccacttcga ctttgaaccc tctcgttccg ccctcgcagt acatgccact
    13441 gcctgaccac gccatggacg cgctgaacgt agttttggcc acctatttca tgacctcccg
    13501 gactgatctc ttttttgtag tataccacat acgtgtatag tgtattcggc catgtcatcg
    13561 aagggcacgc gcccttcaga aagtcggcag atcgatgccg aatgcctgtc tcttgttggc
    13621 gcttgccctt cagttagctt ccattttgcg ttcaggctat actgtggaca cgccagaatc
    13681 acaaccgcca aaaggatgcc agtatccgcc ggtgcacggg ccctcggcta tgaacccgac
    13741 atcatcgata aaccaagtcc ctgcgccagg cttgatgaac agaatgtcta cagtgacgcc
    13801 aggaggaacg cagggtacat aggcactaaa gtagctgtac tgaggctcca ctgggctcgg
    13861 aagcaacacc tccgtcacca cgctgccgtc gaagagcacc tgggcgcgaa aggcatcatc
    13921 cgaattgttg tgaccggtgg catggaattc aaagcgatag cagcatccac atttgacctc
    13981 ggtcaatgtc tgccccaatt tggcagggtt atctacccct gtcggtgttc caaggtttgc
    14041 agaaaatccc agggtatgca cgttcgtggt ttcttctcca acatttcccg caatgttcca
    14101 gccagatggc tcattagcac tatcccaatc gtcaaacgat ccgttggcca ctagattgcc
    14161 acatggacaa atgcgagccg gcggaccagg cggaccctgt ggaccctgtg gaccaggggg
    14221 accctgagga ccaggcgaac cctgcggacc aggaggaccc tgtggtccct gtggaccagg
    14281 cggtccctgt gtaccctgcg gaccaggtgg accctgcgga ccaggcggtc ctggcggaca
    14341 tggcggacat ggcaaacacg gtgagcacgg tggacacggt ggataacaga gactatcctc
    14401 accggatggc gttttgggtt tttggaacaa ccacataccg ttcacctcgt atagaatacc
    14461 tgattcaatg tattcagcag caaaggcatg cggaccagga ctgaggcact tgacaaaggc
    14521 taccgaaacc gcagagcgga cttaacgtgt cggcaaaggc ggtgccaggg tgatggagaa
    14581 gtaggcttgc gcatgtatta tccgccatcg caccattctt cccggagcaa agcggcgaga
    14641 ttattcctgt ctcgttcacc atcggcacat gcctctgtca ggcgtaggga gttccggaaa
    14701 gacgacaaaa agaaaacgcc cctgaatggg cgtccatttt ctgagatctc ctgttgcgtc
    14761 ttgcatgatt gattagcacg ttcgtgtggt atgttagtgg agccggcgaa gggaatcgaa
    14821 cccttgacct gcgcattacg agtgcgccgc tctgccgact gagctacgcc ggccaatcga
    14881 acttcagcag gaacaatgtt atcagcttct gtcctgcatg tcaacgggtt ccgacgtggt
    14941 attgggcgtg ctacgattgt ggcacgcagg gagcgaagca gcccagagat ctgggcgagg
    15001 tgtctaggtt ctaggcacag cccgcgaaag taaactgaag gctgcctata ccgagtgcgt
    15061 ccctagacat cgggttgcaa acgccttctg ctctttgccc atgctccgcc gatcaagaag
    15121 agacagcccg caagcccgat ggcggctgcg ggtttggagc agctatgagc ggcatacaca
    15181 cgtccctcgc caggacctgg aaggggaatg gggtctactg gaataccatt ttggatggca
    15241 agtcccctga tgtctatgag gtttattacc ggcagcccca ttgcggcata ctcgaacatc
    15301 aagccccttt ggggatctac agacattact ttcggaacca tcaccaaacc gttgggaaag
    15361 tcgagtgaag ctgaggttgc cccgtagttg gccgaagccc cacctacgtt tacaaaacag
    15421 gctatgggcc gaccatgctc ggctgcagcc tcttcgaaaa cctgcatgcg cctggctacg
    15481 ctcttttcga tcgaatcctc acgtattacg tacgctcccg agcgctcggc gatctcctcc
    15541 gatatgtctc tcgattcctc gaacaggccg cctccgcctg catcgtcgtc tccgccaagc
    15601 gacacagcgg cgatgtaata agggaggatc ccgttggccc gtatcgcgtc caacatctcg
    15661 atgaaagtaa actctggtat ggtggcgccg tacatcgaag cacctatgga gtatatgagg
    15721 acaggttcga gattcatagc ccttgaagca gccaacgtgg cgataatcaa ggacgggaat
    15781 gaaccgctgg cgcccactgc taccacatcg ccctcccgca gcccggcctc gttgaaatac
    15841 ttcaccatga gggccgcgaa atcgggactg gtggcagtgc gcttcgcctc caaaacccca
    15901 acagtagtgg tgagcggagt gagatcatga ccgatcagcc ctgttccgtt ggggtcaagg
    15961 tcaggatcga tagcaatgcc cagctcggcc ctggcatcgc gcacagctgc gatgcattct
    16021 gccatgattt cggcagctgc aatctgttcc tccacacatg cccctacact gcctcgggga
    16081 tcgccaggca gtccaatgca gtcaggccgc accgaaaccg ccaaggctgc caacgccaca
    16141 agagacgcca agagcaagat gccgaagttg actcgatccc ttccatattg tcgccccgtg
    16201 ggcgccatgc aaccctttct cacccgttcg ttccctccct ctaacaccct caggcagccg
    16261 ccaaccccca agagcgccac ttctttgcgc tgccagcagt tcggtcctta gccgctttac
    16321 aatgtcaaat gcttcttaca acttgccacg aaagcctgtc ggcaataggg tacgtcaaca
    16381 cgttcttcag gcaaaaacga tcataagcag cctaaccact acagcaacca caagcactga
    16441 caggaatgtg cgtgcggctc cctgcctggc aatgtcgttt gctatgaggc ctggaatgat
    16501 gtaaccaatc gcccgcaatt ggggccctcc cggtgtaatg ctcatgccag tcctgttcag
    16561 cagccagccc agccagaaac cgactatgac gcaggccatg aaacgccgcc ttccatagat
    16621 tgcaaggaaa ttcgatatga cccacacaat cccgtaagct aggagggcca ccaggaatgt
    16681 ggccaatatt ctgagcggct gatcaaggta taatgcgaga tacccgggga ccaccaggcc
    16741 tccggcgtat agccctgtca gttcggtgaa gatgaagccg actattagtc caattccaat
    16801 tgagacggtt acggtcacaa tgccaccccg tttgcctcga aatactggat tatcctctcg
    16861 cccatgcccc tggtgtttcc aatcccatat atgacggccc ctgcagggca tcgatctgcc
    16921 acttttgcca atattctctc cgctttgggc tggtgcgtga gatccacgat gacggattct
    16981 gcaactttgc ccctcttaag tccccgtacg gccaccctgc ctgcctcgcc caccacaaat
    17041 gcccattggg gtgaaatact ctccgcgacg aagccggcaa gctcttcagc gcgaaatggc
    17101 ctatcagacc tgttgttcaa aatgacgatg gctggcagcc ccttccggat gaacggggcg
    17161 atcctgcctt ccgccgcctt gcataccagt tctgtagagt cacggtcatt agcggcaaac
    17221 gcattgacga aaacgtaaga ggcctcgccc cgatcgatcc tgagtattcg cgtggctcca
    17281 ggatctccct gggcggtcag cattccttcc agcgccagat cacgcggcac gccgcacaat
    17341 tcacacactt tcagcgcaca tgcaatgttt tcaggaaaac tgatgtagtc gaagtttgcg
    17401 gttgcatcag ccggtgcacc ggatgcgtct accacgtaaa cggacgctcc cgtcttccgc
    17461 gctctatctt caaagacatg gaggaacttc tgctccgcag tgaccagcgc gcctccgtat
    17521 ggaacggtta acgaaagggc ttcggcaacg tcgtgcacgg tcgggcccat gacatccaag
    17581 tgatcgtcgc gcacgttggt tatcacgccc actgtagact tgatcatgtg ccgctccgct
    17641 gcccattggt tttcaggctg aagtgccatg cactccacga ctatcgcgtc tgcccccctc
    17701 gattcggcca acttgactag tctcatctgc tcggagatgt tggcgcaatt tccgcgtcgt
    17761 acaaccggct cttccgagcc gtcctcaaat atgattcttg ccgccgtgcc tgtggtcttt
    17821 gcaatcacgc gcatacctcc ggcacgcaaa ccagcagcta tcaacctcgt gattgtcgat
    17881 tttcccctag tgccgttcac gtgtattcta acgggtattc tgctaagacg gcgcgtgtgc
    17941 aaccagttct cgataactcc ggccacgaaa aggcaggcgc acatcaccgc aaaaccgtgc
    18001 atacatatca cccttttgta tcgatgcaga ctcgatattc tttagatttt cgatgagcaa
    18061 gacgcaattc cttctagcga aatgtggggt catcacccaa gaactttctc atgcatctca
    18121 gtcagtaagg caaatgttgg cttacaacat atactcatct cagtgcctgt atttcatcta
    18181 agtgattccc ataagtaact cccaatgagt tttttgcgct atcggttagc cttccatatg
    18241 atgtctgcta ggagtagaca catctttgca cctatgtcgc agtgcggtaa ttggttcgca
    18301 tttgtgcatc gacaacgcac gcggttttgg gggatcttgg ccttagccat aaactcagcc
    18361 gcaccgggat atgatccccg gggcaaggcc agggtagatg gcgtaatcga aatcccggtt
    18421 cgacgctcgc gggcaaccat cttctttgca accacgaaag cgacgcgcat acgctcccga
    18481 ccatgagatc cgtgtgagcg ctccgacggt ttatgcattc aacgcactca tatgaaaggg
    18541 cgacttgggc ctgccttcaa ggaatatgtc tattatttag tcgcttttcg ggcgcgggtg
    18601 tctaagtttt agactggccc cgtctaaatt ttagccgctt gaagccggaa ggcaatctta
    18661 ggcaggggta atccggcatg cactgtcaca aggacccagt ttccggggat aaataactat
    18721 gcagaagtgc atatacccac atattggctt agagcgcaag cgagtgtcta agtttgagac
    18781 cgctaccgtc tagtttttga acgctttgcg attgcaaatg gctaagactt agactggtct
    18841 gcaaaacctg ctcgcccccg cggcgaacct tggccggctc cacgctgcac ggcgctttcc
    18901 taggcttcta ccgaaggcca gtaaaggacc ttagtagtcc cccggctaac tcgcagacag
    18961 cggcaataca aactcgagga ctgcgaagag atttgttgcg gtctaattag cagtcttgcg
    19021 gtcgacatgg atgatatcac gaaatgtttt ttcaatcata aggaggattt atgtttcaca
    19081 tgtagaagct ttccgtagat attgcgaaaa attacacaaa gtttcgggct tcgtgtaatt
    19141 tgggtcaatg tcggtctatt ggttacattt gacgctaggg aggtgatcat ggcatagaca
    19201 aggcgctctg cttgagcagt cgaaactgta ctcacgattt gaagcatttt atctccagca
    19261 aacaggaggc gaacgtagtt gagaacgaaa tctaagtaca ctggactatt gctcgcgatc
    19321 gtaatggtca ttgcatctgc tttcccagcc tttgcttgca catcagtgcc agtatcgaag
    19381 ggcgcttccg cagatggatc agttatgaca gcgcatacgt gcgacggttg gtacgacgcc
    19441 aggacatatg tcgtgccagg caaaacacac aagccagaag atacgttcaa gtgctataga
    19501 gaccatctgc atgccgacta tgcagagccg gaactgatcg gggaaattcc cgaggcccct
    19561 gtaacataca cgtacttcca cgccggctat ccttacatga acgaacatca agtgatcatg
    19621 ggcgaaacga ccatcggcaa ccggtcagag ctcagcaccg acatagggct tctttatata
    19681 gataccctcc agatcctcgg actgcagaga gcgaagacgg cacgagaggc agtccagatc
    19741 atgggcgagc tagccgagac ttatggctac atcgatggcg gcgaatgcct caccgtggga
    19801 gacccgaacg aaatatggca ttttgaagtt atgcctccag gcaacttcca gaagggcgcc
    19861 atttgggccg ccgtccgcat ccccgaaggc catgttggcg tttcagccaa cagaagccga
    19921 atttgcgagg tcaacccgga cgatccagag aactatatgt gctcatccaa cgtatttgat
    19981 gtagccatcg aaaatggctg gtgggatccg gaaagcggcg aagagttctt gttctggaag
    20041 gcgtactgcc ccagcaactg gtttggatgc aagctccgcg agtggagagt cctctcaatg
    20101 gtagctccct cccttaacct agatcccaat gccgacaggt atcccttcac cgtcaaagcc
    20161 gagcggccaa tcactgttca ggacatgatg aacatctatc gcgatcacta tcagggtacg
    20221 gaattcgata tggccgacgc cccaggatgg tatctgaagg atgccgaagg taaccctgtt
    20281 gtcgacaaac agggtaaccc tgtgaagacc ccatacgcgc agcccaaccc aggcaccgca
    20341 cgaagacagc atataggaat cccggtgaac atgcgaaaca tcgcagttcc ctactgctcc
    20401 tacttcacga tagtccaagc acgcgactgg ctccctgatc cagtgggcgg cctttgctgg
    20461 tttggtctga gcaacccgct gtcatccgtg tatgtaccga tctactgcgg agtcgacagc
    20521 ctgccaaaga actacgctgt taacgacaga aaggtcttta ccttcgacag catgaaggaa
    20581 tcggcatggt gggtgtttga gtacatggac aacctcgtca gccgaagata cgtcgacata
    20641 ttggccgacc tcaacaaggt gagagaaccg ttcgaggccg agcagttcga gcttcagccg
    20701 gtgatcgaga aggctgccgt ggagctctat gagaaagacc ccgctctggc caagacattc
    20761 ctgacaaact acacacattc gagatgtgcc aaggcggttg acacctacta tcgggcggcc
    20821 gaagaactga cgttgaagtg gaacgacaac accttctagt catctggcct ttcatctgtg
    20881 gacgatgatc cgcagaattg tcctcggttt cgccagatgg cgaaaccgag ggctaaatcg
    20941 tagtaggtca acacaagtgc ttcaggaaaa gaaaggagat ttacgcaaca tgaaacactc
    21001 ttctgttcga cgaacaatcc tgattgccat tgcagcagtc atgagcatga ttctgtcaac
    21061 aggcgtatgc ttcggctcag tcagcaagcc cgcagggatc gagattcctg caaagttacc
    21121 caggctcgac cagccggttc tcataacctc aattggacag gctccgggcg catcatgggc
    21181 taaagtgctg atgaagcagc tgaagatcga cgtcacccat gacgatatgg ttaccgccga
    21241 agacttggta aaggcgagca agaacccgaa gaccgcctac aagaccctga taatgtccat
    21301 gggaaccagc ttgaagggaa tgggcggcgc aggggtagat atcgacaagg aagtcgcaag
    21361 atgcaacgcc ctggctgatc aggctaagaa gctcggaatg accatagtag gaattcagat
    21421 agagggaagc gcgcggcgga cggatgagtc cgacgagaaa tcgaacagag cagtcacatc
    21481 gaagtccgat attctcatcg tgaggaagga agtagacaag gacggcttct tcacgaacgc
    21541 agcaaagcag aaggggattc cgatcatcag gaccgttgaa tccatggatt tcaagtacgc
    21601 gttcagcgtt ctgtttagat aatagacaag aaagcttaac gccttaattt gtactaggac
    21661 gaggtggaat aacctgtgta tgctcacgca gctatggtcc tggcggtgat agccgtcgcc
    21721 ttcgtgatcg cgatcctact ccgcttgggc actgaaatat ctatgctcat tgcggcaata
    21781 gcaggcgcca tcgcgcatgg ggcagggatc cctgcccgcc acatcgtgga ggggaccttc
    21841 acatatttcg atgtggtttt catattcatc acagctacgt tcttcatgaa cctgctgaag
    21901 gaagcaggag gagtaaccta cattgtgaga ggcattatat cgaagtttca caagaaccgt
    21961 gtggcgcttc ttctgcttct tacactaata atgctcgttc ccggcgccct gaccggatcc
    22021 ggcacagtga ctgtgctcgt cgtcggatca ttcgtgggca ctgtcctaag ctatatgggc
    22081 atccctaaag atcgggtcgc agccgccgtg ttcatgggat cggcaatgag cgcagctgcg
    22141 cctccgatca acctttgggc gatgatggcg gcggccggcg ctaacatgcc ctatgttgga
    22201 ttcactctgc ctctcggcat aatatctgtc gcaggcgcac tgttctccat attctatttg
    22261 gcgcgcggga gcaagcccat agatgccgag caagctttga aagaaattcc tgaggcgcct
    22321 gaaggcatgg cttggtacaa ggtggcgata ccatttgtcg tcttcttcgg catcatcata
    22381 ctgggcagac agtttccctt ttcgatgccg gttataggcc ttccgcttgc cttcatgctc
    22441 tcagctctgg cagtagtgct cctatctccg gtcaagttgc gagtattcga gatcctttcg
    22501 aatacagtcg aggatcttct gccactgata agcacagtaa caatagtggg gatactcgtg
    22561 cagatcatgg ctctgtcggg cgcaagaggc ttgatatctc tttcggtggt caccatgccg
    22621 ctggcagcca tattcgcggc gctcttcgtg gtgcttccgg tatccgaggg aatattccag
    22681 tatgcttcgg cccctctgtt gggagttccg ttggtgttcc ttctcaacat gaagggttat
    22741 gatcccgtaa tagctctggc gggcatggcg tcaatgtggc cattgggaga ctgcctgccg
    22801 ccgactgccc ctgttggaag gggagctgtc atggctgtgg gatacgatgg agactacaag
    22861 gccttcctca agcagtgcct cgtgccggcg ctgttcgtgt tggtcatagg aacgctgttc
    22921 gtagtcttca gcccaaagct gggatttttg gcccgtggat gaacgaacgg aggaatgtca
    22981 catgcagagc gttataagcg ttttgtacta tgtaattacg gcaatgataa ccgccgtgct
    23041 catgtttaac atgttccgcg ccaaagacat caaaaaggtg atcttgtatg ccgcggtgct
    23101 aatcccattc gcgcttcgtg tagcgcgcat caagtagagg ctgtggaagg aggcggcata
    23161 cacgcatgtc taataagaaa gatcaaaatg gcgtaaacag atgggcaatg ctcattgtca
    23221 tcgtcctatg gcttgccgtc gtgattcccg gcggaaagga gatatacggg catcgccact
    23281 tcgatgaacc cgtagtgctc ggacctggag tcaccgaagt caagaagctg tcagactact
    23341 ctccagcagt caaaggtacc atcaatgact gcaacatata cgtgcttgac agtggcgaac
    23401 ccgggggaac cgtgttcgtt ttaggtggaa cacaccctga ggaacccgcg ggcgtgcttt
    23461 ccgctgtgac aatagtggag aacgtgatcc ccactaatgg gaaaatcctc gttgcggtgc
    23521 gcggaaatta cagcgcatcc acggtaacca ggccgggcga cgcatatccg ctgtattaca
    23581 gcatcaaaac ggattttggc gaaagaacgt tcaggatggg agacaggtgg agcaatcccc
    23641 tcgactcctg gccagatcct gaggtttaca tcaactatcc cagcaagcaa ttgcttgcat
    23701 acatggatgt tcgcaacctc aaccgcacct ggcccggcag acacaacggc atgataactg
    23761 aaaggacttg tgctgctttc atagacttga tcaagcagga aaacgtagat ctcttctttg
    23821 acctgcacga agcagagcta gagtactcag ttatcagcac aatagtggcg caccagaatg
    23881 ctgcagatat cgcgagcatg gcttcgatga tgctctcttc cagcgagttc cacatgggca
    23941 tggagtattc tcccgaaaca ctccatggat tatcccatag agaagtgggc gatttcaccg
    24001 atgctctagc atttgagatt gaggttccag aacccttcct cgatagagtg cgcggagtca
    24061 ccgatgagaa gctgcttctg gaagggaaag acgagttcgt gatgaaagcc ggcgaacacg
    24121 gtctgctcta cgagagcatc gacgaaaacg gatggcccat cgatgagcgc gtcgggcgaa
    24181 ccaactcgac tatagacatg attatcacca tgttcaacga gttcgaaccg ggcagagaaa
    24241 tcggttatgt gaacctgccg ctcagggacg aagtggtagc aaacggattg ggcagctacc
    24301 tcctcgaccc caacagtgcg gcgccggaca gagtagtctt cgactgagcc gacatggctc
    24361 aagtcattat gcataatgca ggaacacatt acgccacgcc tagcactttc aaggtgcacg
    24421 gcgtggcgta tccgttcccg ctcgattcgg gttgggcgaa cgccccataa tccatgaaga
    24481 aatgcatccg aatagccctg gggatggttg ctcaacgctc gctaacatgt gcaacacgag
    24541 gaaaatcaga ccgctgcgtg gaacagtagt accaagcata ccatgccttg catgatcaag
    24601 aagttggtgg agaaattatg atcgaaatcg gcagacactg caatagcagg ttctgggtcc
    24661 caccggagct tgtggaatac gatgcactgt caggcgatgt gaaccttgac cgtacagcgc
    24721 tccccttatc gtccgaacat attgtttttc ggcgctatgg cgcgttgcga cgggcagcca
    24781 atgccctcaa cgccttgcct acaggatcca acgtcctcgc agtagtcggc gcgtcaagtt
    24841 cgaaggcgcc ccaagtctgc tcaatgatcg aagggctaca atcgatggaa gaaccccgtg
    24901 tgcgcctcct cgatgtccgg ggccacgccg accatgctat catccgtgag ggaatagaca
    24961 ttgcccgcaa gaccgatgcc gcactggtta tggcgatcgg cggcgggact accctcgatg
    25021 taggcaaggc gatagcaggt ttggcgctcc aagacggagg tagtgatata gcttcctttc
    25081 agttgaaaaa gatgtcaatc aaccccaagc aagctctccc ttggatcgcg gtgcccacta
    25141 catcaggaac tggctccgaa agctccaata atgccgttgt ggaactgggc gaagagaagc
    25201 ggagcatccg gcctataccc cctccgttca tgatacttgc ggatccttct ctcacagact
    25261 ccctgccgtt cggccctaca gttgtgtccg caatcgacgc gctggcacag tccctggagg
    25321 tttttaccag cgccggagca cccatggggg tgcaatctgt ggccctcgca gcctttctca
    25381 accttgcgga aggcctgaag gcgctctcct gcgcaacaga gagaccagaa tcggatgtcc
    25441 gcgaccggct ctgttggggg agcctcctga tggggatagc tttcgcccat gcccacctcg
    25501 gccttcccca cgccctagtc caccattgcc tcagatttgg cttgagccac ggcaacatgg
    25561 tgggaatact cctggccccg gggctcgaga tccaagcagc tgccgacaaa gagactacaa
    25621 aacggctatg caaagccgaa caggcgttgc tggcattgga tcaagatgac gcttttgaac
    25681 tggaagctat tagagaaggc atggcatcaa acgagcgatg cactgtgcct gatcggttga
    25741 ttgagtggct ggaggatctc atcgaccagc ttttcaccgc cgccgatctg ccaacttcac
    25801 ttggaagcgc cgggctcacc aggtgcgatc tggattggat tgcctcaaac gagcttgaaa
    25861 gcggagcctc atttgggata cctaaccggc ctgcaaccta cggtgaaata atggaggtgc
    25921 tggaaagagc attctaagta agccttgaac atgtgacgga aaagccgcag ggaaaataaa
    25981 tggagcggat gacgggattc gaacccgcga ctcacggctt gggaagccgt tgccctacca
    26041 ctgggctaca tccgctcgta atctacagta tggatcttca ctacacttat tatacgcatc
    26101 ccgagacgga tatgcaagtg tctgtccgtg tccggtctcc gaattgctgt caaatgctgc
    26161 ccagaccgct ccaccactcc ctcggggatc tcttacaaag gccctcagga aagaaacaac
    26221 atcacacgag tcaaatcatg ctgccttcaa ttacgacccg gcatccactc gcttatcact
    26281 ttgcttcgta cgacgtattc gctatgtccc attatggtcg aggccaaaac tgaaagcgtc
    26341 ggcgcgtagc gcctccgagc cccggacgag gctgcttgcc tacagttgtg actctcccgg
    26401 atagcgttta acgcgtgaaa ccgcgacgcc ttcgcccgaa ctgcccgtct acacatcaag
    26461 gccacaattc ccatgaccga gaacggagat acccgatgca accttcttgc cggtcggagt
    26521 caccgctaat ccgccaaggg ctgtttcgcg cagcgacggc ggaagggcgc ggccgatcga
    26581 gcccattgct tctatgacct cgtctgctgg aataacactt ctgattccgg caagagccat
    26641 gtccgcggca gcgagggcaa ccgcaactga tgtcgcgttc cgcttgatgc aaggcacttc
    26701 aaccagtccc gccacggggt cgcagacgag gccgaggagg cccttgaagg ctatcgcaac
    26761 ggcactggcg caagcctcgg gggagcctcc tgcaagctgc acagcggctg ctgaggccat
    26821 ggctgcagcc gacccgcatt cagcctggca tccggcggcc gcccccgaaa gcgtactgct
    26881 tcgctcgatc acagcgccaa cgcctcccgc ggcaaatagg gcctccacga gagctaaatc
    26941 atccacgcat agcctctctc ccactgagag caacacaccc ggaagcaccc cgcacgatcc
    27001 ggcggtcggc gcggcgacaa tccgccccat tgatgcgttg acttccgcca cagccaaagc
    27061 ataagccagg gccgaatcga atggctctcc acagatcgtc tccccgttca ttctggcaga
    27121 tgccatgcgg ggcccgtccc ccccagtaag gccgcttctg gaccgcactc ccgcaagccc
    27181 ccggtccaca gctgacctca taactccaag ccgctccctc atatcggcgt caagatcatc
    27241 ttccgggcgt ccgctctgct cggcctctct ctcgcgcacg attgcgccga tgctcacgcc
    27301 acggctagaa gcttcctcca ctagctccgc aagggtagta attgccatgt aaattccctc
    27361 ctaggccaat gacaccggcg gcagcgatat tgctgtgtcc acaccaacga tgcgcgagat
    27421 caacgcccgt gcctcgtccg aaaccggctg gtcaacttcg ataatcgcca acgccttcgc
    27481 gtgttttgct tcacgagaca cgcgcatggc agctatattc acatcttcag tagccaacaa
    27541 agaggtgatc ttcgcgatta tgccaggctg atctctgtat gcgacgacaa tagcatggct
    27601 ttcaccggaa aagtcaacct cgaaatggtc gatcttggtg atgaggatac tacctccccc
    27661 gatagatgag cctactaccg aagtggtcgc gccgaaaggc ccaaccagct ccagtttggc
    27721 ggtgttcgga tggatatccg gcgacgcttc cgcgacctcg aatttcacat ccagtccgct
    27781 ctgagctgcc agacgccggg cgtccggaat gcgctcatcg tccggactca tccccagcaa
    27841 gcctgcaatg agcgccaagt cagtaccgtg gcccccgtat gtcgtagcaa aggagccgta
    27901 cagggttatc acagcccggg aaggttggcc gccgataatg gcgcgggctg caagtccgat
    27961 tcgaaccgcg cctgcggtgt gcgaagaaga gggcccaacc atcacggggc cgattatgtt
    28021 aaagacagtt ctcatgcgct cgccctccaa gttgccgaca gatgcctggc ccgagcatct
    28081 ttagctccac cgatatctac atcctcgaaa cgattgaacg cgaggagtgc tggtgataag
    28141 attgttcata ccaacctcga tcgaaccaaa tcttctggag acatattccc ttgtcctcgg
    28201 cccagcatgt gcactggcaa gtctgactta attatgtcag cctatcgcct ataagtcgag
    28261 aggagaatct ccttgggaaa tagcagagaa ggtattctga atggcttcga cctgaaactg
    28321 atcgctgcgg ccctgatggt gctcgaccac gtactcgagt atttccctag cgccccaata
    28381 tggtttggct atctcgggcg tatagttgcc ccaatattct tctacctgac tgttgaaggc
    28441 ttttttcaca ccagaagcag ggtcaactac atggccaggc tgtttctggg ggcagcggtg
    28501 atgtatgttg gctcccgcat tctgacgcat ttcatgccga ctccagtggg gatcccgaac
    28561 aacatttttg tttcgcttgg gcttggcgtc gctctcatga gcgcattcga ctggacccgg
    28621 gagacgggca agtactggac gggaattccg atgattattg cgatcgctgc cgtatcgctg
    28681 tcaacagaag catctttata tggcgtcgca atgatctcag tattctactt ttgccgcaac
    28741 aacaccaaga tgatggcaat catgtacatc gcaacctcgc tcatgatctc gtggccatcc
    28801 ccagctttga acaagacgca tatcctgctg tacgattacc aatggatgat ggtatttgca
    28861 actccattct ttttcctcta caacggcaaa cgaggccccg ggggcgaggc ggcaaagtgg
    28921 ttcttttaca ttttctatcc agcacatcta tggataatat atgtgattca atatttcacg
    28981 ttctgggtat aaagtcgaca acaggctaga agctttttgg gccaaccgcc gcagatgcag
    29041 cgcgggccca aaaagcaccg attacgtccc cgcctccaca ccttccggtc cgttcgacca
    29101 ttcaatccgg aaacaggttg aacaggacct cagcctgtca cctccaagtc tccttggagg
    29161 ccgccttcac cgtcgacctc ccggatcctg aaggaaccgc ctttatcaat cctgagatgg
    29221 gcttgtatac gagcgcggtc aaaaccgagt tgatccccgc tttaagcacg ttgaagagca
    29281 caatccaccc gatcatgtcc acaacaacct gtcttggcat cccatagaac atcggaatca
    29341 ggaagatgtt agccgggatc atcgcggcaa ccatagccac cccgccggcg agcaatgcca
    29401 agtaagcgcc tctcttcgtc ttattcctgc tgtatatcat cgcagagact gctactaagg
    29461 cgccggacga gataagatgc atgaggcccc caaaccatcc atctgatgag agcagcaaag
    29521 cctgaaacac gcaggcgact gcggtgcaca ccagtcccga cacaggcccc aaagcaaacc
    29581 cggctacgag gagcgccaca tcagccggtt catagatgag gaatggcgca ggcggaaaag
    29641 gaatcctgag cactagaagc atcacaatgg aaagagccgt aagcaccccc attgtcgcta
    29701 tgcgtctgta ctgtgcgcga ctattctgac ccatgacgat acccccccgt ggaaaaatgt
    29761 atcgaaaaag cccttgggtt gtggcccagg ggcttaaggt cgactcgctt ttgcaagacg
    29821 ccgccttctt tcatccggac tcttaccgtc ggtcctggat tctaaccagg tcagccgcat
    29881 tgcggttcgt ggactcgcct ctagtacctc gcatctttcg ccagatgcgc cgagaagctt
    29941 caccaccggt cgggaattac accctgccct gaaggccacc gtattcgctt tttcaatcaa
    30001 tatgatagca cacgcccgat agataggcaa ttggagttaa ccgagcgtta gaatgctgta
    30061 ggcacctgtc gaacttgaag acggaaaagg ccaaagcaat ccggttttcc acgcttgagg
    30121 ccatcattgg attgccggcc aggcgacatc ttaaaatact cacctgatgc cgaaggaaca
    30181 taggccctgc tcgctgcggc cccgaacagg atgatcaccc gtatgcagcc aaatatcatc
    30241 cctgggggct ataagcaccg ctcagaagta tcctgtataa taagtaccga ccttctaata
    30301 cacctttcac tttgcaagca tacaaatatc ggcccaattt ggcaggagcc tgcattcttg
    30361 cccaggcaac aagcgagtct caggaggtgt ttacctgtgt cgggagatga aaagcaacgc
    30421 cctcgcaagc gttggccctg gatcgttgct atattagtga tcgccgtcat cactctcatt
    30481 gtctcttctg gcaagcacga ccgcgacgga gtgtcgacgc gcttggccgc agccgtagcc
    30541 ggccaggaaa ccattgccgt atcaactggc gatgttcatg agttcgtttc ggccgccggc
    30601 cacctcatgc ccattgccac aaaaactctc tcatttgaca tgccgggaag gatcacaaga
    30661 ctcaaagttg aagaaggttc ccgtgtaacc gcgggatcgg tcgtatgcga gctggaggcc
    30721 aatagtagcg cctctctgcc tggcgactac ttgcgttcac tacaggagtg ggttcccacg
    30781 cccgacaccg gcgccgcgga gaagaaactc gccagtatac gggcaccctt cgacggagta
    30841 gcggtgtcag tcaatgcgag cgtgggaagc tacgtaacgg caggccaacc cgtgatcacg
    30901 gtgatcgaca catcctcgca cgtagtcgat atagagatag atgagataga cctcactagg
    30961 gttagcccag gcatggaagc gactataaca ttcgacgcca tccccgggct gaagcttccc
    31021 gggaaggtcg attccatcgc ccaaatggct gttcccaaag gcggaatcgt gaccatccct
    31081 gtaaggatat ctgttgatgg atggcacgaa ctgctcaagc ccgggctaac ggcgagcgta
    31141 aagattctgt cgcgcactct ttctgacgta gtcaagatac cgattcgctc ttggatcgat
    31201 cacgaaggcg aaccgtcggc aataaggatt gaagatgata ttcccatatt cgtccctctc
    31261 gaacttgggt tgtctgatgg cgaatacact catgttctct ctggcctcga gcctggcgat
    31321 atcatcgtac tggatgcagt cgccgtccag gaaaaactga ggcgcgcagc ggggcaagat
    31381 cgtttcatca cctttgatgt ccagcgcatc gacgattgaa gcagggaggc ggttttcgtg
    31441 gttgatctgg aaaacgtgac caagatctac tctatgggtt cattcgaggt cacagctatc
    31501 caggatatca ctctcagcat tgccgatgcc gacttcgtat ccataatggg gccatcagga
    31561 tctggcaaat ccacgctcat gcacgtgctt ggctgtctcg accggcccac ctcaggccgg
    31621 tatcgcctat gcgggcagga tgtgtcacgc cttgacgatg caagcctggc ccaaatccgc
    31681 aacaagcgga tcggattcgt gtttcagcaa ttcaacctgc ttccacgcga aacgatcctc
    31741 tcaaatgtag aggttcccct catatatgcc ggggtgaagg ccaaggagag gcggcggatc
    31801 gcaacggaag ctttggagcg agtaggcctt tctgaccgaa tgcgtcaccg cccttctgag
    31861 atatcaggcg ggcaaaagca gagggcggcc atagcgagag cacttgtaaa caatccttcc
    31921 cttattctcg ccgacgagcc cacggggaat ctggattcga agactggcca tgaaatactg
    31981 gacatactat cccagctgaa ttccgaagga gcgacggtag tccttgtaac gcacgaccgc
    32041 gagatagctg aacgggccaa tcgaatagtc cacctgcgtg acggggcaat tgagctcgtc
    32101 gaagaacttc gtaaaggagg cggtgcggat ggggtttctt gagacaatag gcctcgcctt
    32161 ccggaatttg tgggcaaacc gtcttcgcac catgctctcc gccctcggca tcgtaatagg
    32221 cgttgcttcg gttatcacca tgatttcagt aggaaccagc gccgagcggc gcattgcctc
    32281 ggagatagcg ggcttgggat cgaacatgat ccttgtaacc cccaagatcg ggcgaggagc
    32341 caagtccgca agttcgccat ctctcacaat cgagatggct gaggagctga aggccgcggc
    32401 gcctgcggtg tctgccgtat ctcctgtggc gcagatcgaa gggcagcttc gctgggcaag
    32461 ttcaagcaca aacacttcaa tcatcggaat cacctcggat tacatgagga tagcaaacca
    32521 gagtattgtc aggggaagaa gtctaatccc cgctgaagtg gactctgcgg ccaaatccat
    32581 cattcttggc gccaagactg cctctgctct gttcgaagat gaagatccga taggccgcga
    32641 gattggcgtg gtggcagcag gccgtcggtt cgatttcacg gttgtgggcg tggtagccgc
    32701 caaaagcgat atgctgttca tgaactccga cagccaggca ttcgtcccaa tcacttgcct
    32761 gctgtacagg atgatgccca tccgcggaat aagcatgtac tattgccagg cccagtcgcc
    32821 ggagatggca ggaccggcag ttgcccaagt cgaaagatac ctggcatccc gcaccggcaa
    32881 ccccggcgcg ttcaacgtag gttcacagga agcactttta gatgcagtgc accaggtaac
    32941 atccacgatg actctgcttc tcggaattat cgcaggcatc agtctgttgg taggcggaat
    33001 agggataatg aatactctgc tcacatcagt ggcagagcgc acccgggaga tcgggatcag
    33061 gaaatctctc ggcgcccgca atcgtgacat actccttcaa ttcctcgttg aagctctggc
    33121 cctcagcctg atcggcgggg ccataggcat gattttgggc tggatggcag ggtcaggcat
    33181 ttcacggctg gccgggtggc ctccctcatt cagcatgaca tctgtggcgg cagctgtggg
    33241 tttctcttct gctgtagggc tgatcttcgg catcttccct gcagtcaagg cagcgcagat
    33301 gaaccccgtt gacgctctgc gatacgaatg aacgcgttac ggcagaacat atttgggcgt
    33361 gggacctaag tgcaatatgg cctcttcgat ctatggaagg gttggcggag tcatcgacgg
    33421 acttagtatg aactgtgcaa ctgactccgg atcaatacat ggacgcatcg gaggcgacat
    33481 attcggcgca gacatagatc ttgcagtaga cgagcttagg ggaacagtct caggccgcat
    33541 aggcggcgtc atcatcggaa ggtccgtgga cgcgaagtat catgctggga gcaacgcaag
    33601 cctttacggg cgcgtaggcg gaatcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33721 nnnnnnnggc ggcaggatta tcgcatgttg catgcccacc ccgccgccat cttgctcatc
    33781 tcagattctc agtacgtgaa cagaggccat acgccgtttc cgtcgggccc acgatagatc
    33841 acagggttct gtgtatgaga tacccgctct gcctccttcg gcacatagta ctcgacatag
    33901 tcgaatatct cctgcgtagt tatgattcca tcccgaacat ggttgaaaca tccgtccgcc
    33961 ggatacggca cctggccgta cggctccatc ccgccagcaa ataaccatgt aaacaggccg
    34021 ccgttccatt ggggtccttc gattgactgc tcgttcttgt cgcacgctgt aatcacgatc
    34081 ttgcccggtc ccacgatgtt ttttgccatg atttcaaagc ccgtcgaacg ggtgaatccc
    34141 gcgggcatcg attgatccaa aaccttgaac attccgcctg agtagcaaga atcgaatacg
    34201 aataccttat gtattgcagg gatgtatctg tccagataat catttagctc atagtctgct
    34261 ataagtgaag tcaggtatcc atcgtagtca tgcggaacga tgtattcggt gttcgtactt
    34321 gcattctgat atccgtggcc cgaaaagaac attatgaaag tgtctgacgg gaccatctcg
    34381 cgccccatcg atgctatcgc accgagtatc ccggccttcg tcgcgtcgta atcgcagagc
    34441 ctcacggtcc ttgcaaccag tttgttgtcg acgccaagca cctgcgcaat gagcccggca
    34501 tcggacgccg cgtacgtcag attaggcgcc aggctcggat ttctgtaggt acttactcct
    34561 acggaaagca ggtgtaggga gcccataggc accgtgccga catatgtggg ctttccggcc
    34621 tgcacccgac attgaattgc gtatcccaag atgaagcgat catcggctat ctttactacg
    34681 tactctccgg gttcaacctg cgacattctg aagtcgccat agcggtttgt cgtcgcaata
    34741 tacgtgcgcg acgggtatcc tgtgctctca gctaccaccc taacgcccgg cagcccaaca
    34801 taggacgaat ccgcaggcgg ctccggcacg attattactg ctttatcttg cagctctggg
    34861 ctggaaaggg accttgccat tgaatgtttc acgacacatc ccgcgattga ccctgtagtt
    34921 gttgaagata tcggaccaca cccggccacc aataaagagg tcacaagaat cgcagcaaga
    34981 actgccgctg ttcttctcag cttcatgatg cttatctcac cccccatgtt tcacaacatc
    35041 gatgttgtca ttgtgtatgc ctattggacg agcatatgca gcaatctgtt ccttagaaat
    35101 catcttctgt gcaattgccc atatagcgct gtgaaggaga atgatgcaca gctgaggaat
    35161 atataacttg tttctaatga attgctttga gctatgctgg ggtgatgtgg ctatgtcgcg
    35221 atcgcgactc cttactctat tgtgcgttgc actgatttgc gcgacgctga ttggtcatgg
    35281 cccaaacacg gctgtggcta tgccctctca tcagattgcc agccgaggac agacgctggt
    35341 agaaagggaa tctgattctc aatcgattct caccctcttt cccgagttga agagccttac
    35401 tgcccccctg tgggtgcagg aagggctaag agcttactat cggacatcgg cggcgacggt
    35461 gcctaacccc ttgagtgggc cgaacaagga cgccgtgaag cctccaatca tgcatccaga
    35521 ggagcttagc cctgcagccc ccgctctggt cagaactgat gtgatcgccc tcgctacgcg
    35581 gttttcggcc acctgctcca ccctattcat aggcgaccta atgataccgt gggaagacgt
    35641 aacacgggcc gcctatgcag gcgcaggtga tttctggata aaccccatag tgttcaagga
    35701 tattgccaaa cgcgctcccg agcacctttc catctccgag attcaatttg aggcaggtgg
    35761 aacaaagtac gattccatca gattcgacta caggcccaca ggcagcaaca agcattatgt
    35821 ctggatattc cagtccagca cggggctcct catctatcag gcgcaatggc ttacactctc
    35881 ggatcaaaga ttagcttacg tttccgcagc cgagtttgtg acgaaacgta acatctctct
    35941 tccttggtca ggagaagccg cgcctccctg ggcaaaaccc ggcgccaagc tgacctatca
    36001 gggcaccata aggacatggc tacccacaac cggtcacaac attcctatac ccgccttgct
    36061 cgaagttaaa attgaagatg ggggccgaaa ctggacggtt gccaaggtta cccgagtctt
    36121 ctcggatgat agggggcaga cgggcgccgc gatcaaagta tattcgggaa cgggccaaat
    36181 aggcggattc tggctaccca aggcaggtct ggcaaagctt aagaatggca acaagctcga
    36241 ccccttcgac ccgctcgttg gaagttccgt cgaagtaagc tatgtgggca aaaccccgag
    36301 cggcatttca gtggcggcaa tattcgaatg gggcgcgaaa tacaagagag tctggatata
    36361 ccgttccacc gacggaatgc tcctttactg gctcgacgaa aaagtggtgg accccgttac
    36421 cggcatggtt cagcaagccg agtggcaact catggaataa tgagcccgcc cgagtggctc
    36481 aagctgaatt cctccggaga agaatcgaac cagatggcgg caggcatgcg aacgaacctg
    36541 ccgtctttta catgtccggc gtcccaagtc gtatcgagca acactatcct gccattcact
    36601 tccgcttcgt tccaagcatg gctctctcgt attccttcct gaatcccttc acccccgact
    36661 acgcgcacgc gaagcccagc cgcagcagca agagcggctg tgacatttgc atatccctgg
    36721 cacacacttc ccccgcgggc aatgacggaa atggcgtcag tcggtccaaa gtcgccgctg
    36781 gcagcagcgc ggacgtcgta ggcaaggttg gaagcaatcc aatcgtgtat ggcccggagt
    36841 ctgtcgtaag gatcacgcgc atccggcgct atcgattccg caagcaccct gatttcggca
    36901 tcatcccatt gaatctgtct tttctccgaa tcgtcgagaa gagggatttt cggaacccga
    36961 actaccctgt acttgcgccg gggcacacac ctgttcgtgt cgcgatgaag cgaagatatg
    37021 gttaccaggc actcctcgcc gattgcgggc ggatctatcc aaagtccaaa tgcgcctgcc
    37081 ctggaccgtt ttagagaggc tgcacttttc ccatcacgct caacatccac cctgacgccg
    37141 tcggcgttgg caacgccctt aataaggaat ctgtcgcaag ccagtatcgt tccacctcgg
    37201 tatgcaggct caatcatgtc gatgggcgca ggatcgacta cgaccagata ctcggcaacc
    37261 gtgttgtaag tgccaggagc aatcaacctg gacgccaccg atattcggca ctctccctct
    37321 cctacatcca gaggaatggt gatcgcgaaa tctccgttcg gcagtgtttc ggcgatatga
    37381 tcccgtctct gccccgtttt ggcattctcg atactcgtcc acactgctgg gaacgcactc
    37441 ttgcctgtga tcgtcacgaa atcagagact ctgttggaag atgcgatggg agagttgatc
    37501 tcgagatccg agccgtagcc cgaatattgt ctcggcgcca aaggaatcgt agacaaagta
    37561 ttgtcgacaa caagacggca ggttaccctg aaaaccgtct cgcctctggg aaccgcggct
    37621 atgtccatta cgtatcgacc cgggccatcg aaaaaccaca ctgtcgctga gaatccgcca
    37681 tcatcgccaa cagtcacaaa agtgttcgca aacttcgccg gatcatcctg ccgggaacac
    37741 ctgatcaaga ctgtgctgca gcatgacctt ccgctcacct ggatggttcc aggaaccgaa
    37801 atgccggata cgggaggtga gctgagcgtg agcagatcgc cgtatccagc gaaggtttgg
    37861 attgtcgcag cctcctcctg cttcggggca ccgccggctc cgggagacaa tcctaaggct
    37921 gcaacaagcc ctgctatcaa taacgtcaaa actcttcttc tcgcgccaac catcttcatc
    37981 atattcactg aaacactcca tagccgccac tatccgatgc aagctggcgt attatcacgc
    38041 tggccatggc aagtccagca gccggtggaa ctgttgatat gctgcctatc ggcccactgc
    38101 tcttgacaac gggcggttca tcagaaaaga cgactgtgag gccggtttcg atcccgcgcc
    38161 tgcgcaaccc ctgtctgaca ctcctggcca tagggcacgt gtgagtcttc gatatgtcgg
    38221 ctattgcgaa cgccgtagga tcaagcctgt ttccagctcc catcgacgag actatcggga
    38281 tttgcgatct taagcaagcc tctatcagat cgagtttcgc cccgacagag tcaatagcat
    38341 ccgccacaaa gtcgggacga agcctgagca agtcgccgac attgctcccg tctacccagc
    38401 aaacttcggc cactgcctcg cattgagggt ttatcctacg cactcgttca accatgatct
    38461 cggctttggg ccgcccgata ttatcagtaa aagcaatgat ctgtctgttt atgtttgtgg
    38521 tagaaaccac gtctccgtcg acgaagatga gcttgcctac atgcgcccta gcgagcgcct
    38581 ccgcacaagc cgatcccact cccccgagcc ccacaacagc aactgatgaa cctgccaggc
    38641 gggccactcc atcagcgccg atgatcagct cagtccgttc tgtccaattt gcatcttcca
    38701 agttgtaagt catcccttcc agatccccgg cttttggtct atcatggagt ctgccaggat
    38761 ttatccgcgc caacgtagaa gaacgtaatc atcgaagcga ggtgtcttaa ggttgagcaa
    38821 ccacgttgca actgagatca tcgatattcc caatagcagc gacaccgtca ttatacataa
    38881 tcgcgactgc attgaaggca tggcccagga catagcttca ggatcggtgg atgtagtagt
    38941 tacttcaccc ccgtacaacc tgggcatcga atatgggaca tacgacgacc gcatcgaccg
    39001 cgacgaatac cttgaatgga cccgcgcatg gacatctgag gtggcgcgcg ttctctcgcc
    39061 aaaaggatcg ttcttcctca acataggctc caagccgagc gacccatggg ttccttttga
    39121 agtcgccatg gagctgaggg aaaccttcgt tcttcaaaac gtgatccatt gggtcaaatc
    39181 gatcgctatc tcccatgaag atgtcggaga ctacccggca atccgcgaag atatagcagt
    39241 ggggcactac aagcccataa acagcaagag attcatcaat gattgccacg agtacatatt
    39301 ccacttcacc cacaacggag acgttaccct cgatcggctg gcagtggggg tgccttacca
    39361 agacaaaagc aatatcaatc gctggacccg agaaggcagc tcgccaatcg atctgcgctg
    39421 ccgtggaaac acatggttca ttccttatga gacaataacc aggcggagca gcgaacgccc
    39481 gcatccggcc acattccccc cgaaactccc ggagatgtgc ataaagctgc acggcctcga
    39541 ccgatgcaga ctagtgatcg acccattctt gggcttgggc aataccgcta ttgcctgcgt
    39601 acgcctggga atgcctttcg tcggctacga aatcgatcgg ttttacttcg ccaagagcgt
    39661 agaacgcgta actcaagccg tgaaagaaag ggaaataatc gaggatgaca gcagggtcca
    39721 ggtcacccag caaagccttt ttccggagca tgatcaaacc gaaaaaaacg ctagcctata
    39781 actgcacgaa ctgcccggag caggttttcc cccatgatcc cctgtatcgc tgattcagag
    39841 tagccgcggg cagccagttc ttccgctacc tttccaaggc ttgatacatc cttgagctcg
    39901 tcagggagct gctcatcggg caggccgtca aaatccgaac ctagcgctat gcactccggt
    39961 ccgattaggc ttgcgaaatg gtctatgtga tcaacaacgc cggcaaggga ccttctttcc
    40021 tctcctagga agtatcctgc gaagtttacg ccgataaccc cgcccttttg ggctatcgct
    40081 cgcgcctgat cgtccgtgag gtttctcggg tgatcgcaga gggttttcga gtttgagtgc
    40141 gaagcgatta caggcctctt cgacacctcc atcacatccc aaaacccggc ttccgacagg
    40201 tgggatacgt ccacaaccat cccgagcctg tccatttcct ccaatacctc cacgccaaat
    40261 cttgtgagcc cgcctcctgt ccgactctcg aaagccccgt ccgcaagctc atttctgaga
    40321 ctccaagtca acgtcatgag ccttacgcca agacgataga aaatgcgcag gagctctatg
    40381 cacccatcca gcgcctcgcc gccttctatc gacaatagcg ccgctatctt gccttcctca
    40441 tatgcctctt ctacgtctct gccactcgtg gcgaagacca gcttgtcgct gttctcctcg
    40501 atctccctgt agaacgcatc aacgtactgg agagccctcc tggcaccccc gccaggcaaa
    40561 tactgaggag gcacatatat ggcgaatacc tgcgccgaaa ctccagcttc catcatgcgc
    40621 gccaggtcga gctggccgcc cttgtcgcga tctgccaacc tgcggcggcc ttcgactatg
    40681 tccatgagcg tatcgcaatg tgcatccatt atgaacatgt actgcacctc caacgcttgt
    40741 gcaatcaata catattgagt ataccactac aaccgcccac cagcattgtc ccgttttggc
    40801 tgaagtgcta atattgggca tgagccgaat agtgcacaaa agtgttcacc cgggaggttg
    40861 atcatgcatg ccgaaaatca agattgtcac cgactctaca gcggacttag gcccggaact
    40921 gatcaaacgc tatggcgtgg aggtcgtccc gctcatagtc acatttgatg aagaaacata
    40981 caccgacgga gttgatatcg atactcaccg cctgtttcag cttgtcaagg agaaaggaaa
    41041 gcttcccaaa acctcgagcc cgagccccga aacctttaga tcggtttttg aaaaagccat
    41101 cgccgacgga agtcaggtaa tctatatagg catctccagc cagttttccg cttctgtcca
    41161 aagcgccagg ctggtggcaa acacattccc cgaagggcag gtgcatgtgt ttgattcggc
    41221 caacctgtcc acgggaatcg cacagcttgt cctttacgca catgatctag ccgccgaaaa
    41281 caaaaccgtt cccgagatca tggacctcct cgaaaaagca cgccccaagg tcaaaacgtg
    41341 cttcatgatc gacactctag agtacatgca catgggaggc agatgcacgg ggatccagac
    41401 gttgcttaca tcagttctca aggtaaggcc cataattgaa gtagttgacg gaggcatgag
    41461 ggtggcggca aagatacgtg gaagcaggac caagcttctc gactggcaac tcacacgatt
    41521 cgcccaagat gtcgagcgcg ggctcgtcga acctgtccgg gtttttctga ctcatactgg
    41581 ggttcacgaa gatgccctgt acatggcgga tagaattcgg cgcatcatgc ccgagatgcg
    41641 cgagatactt gaaacgccgg caggctctgt gataggaagc cactgcggtc cgggcaccat
    41701 cggaatcctt ta
//
LOCUS       DAOSYL010000013        41299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_776_length_41299_cov_8.486010, whole genome shotgun sequence.
ACCESSION   DAOSYL010000013 DAOSYL010000000
VERSION     DAOSYL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 41299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41299
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_41299_cov_8.486010"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..2009)
                     /locus_tag="PLU15_03810"
     CDS             complement(177..2009)
                     /locus_tag="PLU15_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOO30078.1"
                     /translation="MRYRSAAVIVSLAFILIAGFNPAFGATANVSTPGLGFEGAEVAI
                     LDSGYTRHYADLKAWTYSEEFIDATWLMSTLARSGISAAVINDHDVEIGALSSYKALI
                     LPATSCMSSLAIDQIEAFATAGGRVFSTYDTSLRDDFWRTRTDYGLSSIMGVRYSSTG
                     TSGNNHIVPAAKSHPIFRGIESDIPVPSTYGLTNKMNPNAIILAKWSTGRSAIVETEY
                     GIYCSENLFARKNLSSPEVVTLITNTIDYLVNTKYKAKFNLGRLDTRATWYRPAAREE
                     QIRADVKRLANANFNLVFVDSFYDGYTIYPSDVGVQNPIYSEFDPLAIAIDEGHKHGI
                     QIHAWLETFCVGFASEKETPPQILQENPDWAAVGLDGRIPSAAESDRCFLSPAHPQVQ
                     EFILDLAWELVTEHAVDGVHFDYLRYPYGRTIPYDYAPTITKLATEDLGFQPRDVRLD
                     IAKWNAWFDWRCNNLTSFMIKLSDAIRSAAPGTLVSAAVYPYPEAILTRMQDWKAWCE
                     KGCLDFVVVFTYTQDLDLFDTMIRAAQSFAGNKTQMIAGIDADSMTHSQAGEGVPALV
                     EAARAAGARGVSIFSANAISENVLTSLKSGPFDPSTKSETPSYP"
     gene            2593..2880
                     /locus_tag="PLU15_03815"
     CDS             2593..2880
                     /locus_tag="PLU15_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30079.1"
                     /translation="MLSDITGDSIYLDVARGVVNSWIDKVLIRRQDGVMDWNWDYGGK
                     VGKLNWTAEEFDKTQGAFVSGFWATRDGHEWVCSVRRLRSAPALPSLLTLF"
     gene            3262..4305
                     /locus_tag="PLU15_03820"
     CDS             3262..4305
                     /locus_tag="PLU15_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO30080.1"
                     /translation="MNRIRKSSMLLSIVLLLALMMGIGIPMEAAAKPRVAIVFATGGL
                     GDKSFNDSAYDGMKRAEKDLGIEFDKVEPQAVAEYETLLTRFAQTRKYDLIISIGFDQ
                     ADALKVVSARFPNQKFAIIDTAVDAPNVASYVYKEAERGFVVGAIAGLMTTKKGDPRI
                     VSDKQVVGVIGGMDIPLIRANVAGYIAGAKYVNPKVQVKYAYVGDWADPAKCKEMANA
                     MFDEGVDVLWLAAGRSGLGGIKAAEERNRYAIGADSDQGYLAPRNILTNGMKLVDRTV
                     YIAINDVLRGKFKPGVNMLGLKEGALGYSKSLVPADVMSQVDKLAAKVVSGDINVPDE
                     IDKVEPWLKAQGK"
     gene            4407..5921
                     /locus_tag="PLU15_03825"
     CDS             4407..5921
                     /locus_tag="PLU15_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30081.1"
                     /translation="MYAVRMENITKRFGSVTANEAVDFAAFPGEIHCLLGENGAGKST
                     LMRILFGLCRADSGLICIDDEPVDISGPRDAISLGIGMVHQHFMLAERLTVEENIIAG
                     CEPSKNGLVDYDGARERILEVSQRYGLTVDPTAVVEDISVGQQQRVEIIKALMRGAKV
                     LILDEPTAVLTPPEVEELFVVMRALRDAGTTIIFITHKLKETMEISDRVTVLRDGRTV
                     GVVNTADATPDSLVEMMVGRSLPIHDSHRVPAKDGIFLQLKDVSVAGKKGSRARLDSV
                     NIAVRGGEILGIAGVDGNGQLELEEVATGLQRPSKGQIIVNGQDVTGSDTRSIRDSGV
                     AYIPSDRLKRGMVDSFSIARNSVLGMHHSAHFSHRGVIKEDVVRRHAENLVDVYDVRS
                     ESIDQPIGSLSGGNQQKLVVGRELLRPHSVVVAAQPTRGLDVGATHYIREQILASRNK
                     GKAVLLISAELDEILELSDRIAVIYEGRIVDVRPTSQYTERELGLLMTGQRNGV"
     gene            5925..7031
                     /locus_tag="PLU15_03830"
     CDS             5925..7031
                     /locus_tag="PLU15_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30082.1"
                     /translation="MDISGDASNKLSKKNAEWFARPGFARFVYSAAAVVLALLIGAVL
                     IGVSGYSVKEAYYNLFMGAFGGVYSLATTIVMATPLLFTGLAVAFAFQCGLFNVGAEG
                     QLYVGALASAVVPLALEGLPPVLRVIIALLAGAGAGAAFGFLPGYLKAKIGAHEVITT
                     IMLNYIATLLTTFLVKTYFRDPGPVDQTVLLPLGSRLPEIIPGTRVSLGIVIGILAVA
                     LLNYVIRRTVLGYEIQAVGQNPHAAEYGGVNVRARMVQAMMISGAVAGLAGSVEAMGT
                     LYRFIPYFSPGYGFTGMAIAVIAGESLWAVIPAAILFGALQSGGQSMQLFAGIPMELM
                     TMVQGLVILFVAAPGMAEMIFRRKSCGKEGGGRA"
     gene            7028..7975
                     /locus_tag="PLU15_03835"
     CDS             7028..7975
                     /locus_tag="PLU15_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30083.1"
                     /translation="MSGFVDIVFNVNNLAATIRMATPIALAAMGGAFSERSGVVNIGI
                     EGMMLTGAFCGVLGSHLTGNAWLGVLFAIVGGGLVGALLAVFAIRLKADHVVTGVGIN
                     ILASGATTWLMQVIWKRKGASDLVAGLGTWSVPGLCDVPVLGTILGTHSPLVYISFVL
                     IFLGWVLMFKTPLGLRIRMTGEHPEAADTVGINVHAIQYFCVILSGCLAGLGGAYLSL
                     AQLNWFSKDMSAGRGYMALAACIFGGWNPLGGLGASFLFSFTDAIQMRMQTAQISLAV
                     EIIQMIPYILTILILASAVVRSRPPAALGKHYESGHANK"
     gene            8194..9054
                     /locus_tag="PLU15_03840"
     CDS             8194..9054
                     /locus_tag="PLU15_03840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOO30084.1"
                     /translation="MSCADVRHIDASEIAEAVYKLCMKVTCVLPPENIAALERAMEEE
                     KSPQGRQALARILENHRLAGELMQPACQDTGIAVVFIELGQDVHVCGGDLNQAIDEGV
                     RRGYSEAYLRKSVVHDPLFGRVNTKDNTPAIVHLQMVPGCSLKITVVAKGGGAENMSG
                     LAMLTPASGVEGVRNFVVDQVRKAGPNACPPVIVGVGVGGNFETVAFLAKKALIRPVG
                     ERNPNPKYAALEEELLREINGLGIGPQGFGGSVTALAVHIEAYPCHIASLPVAVNLNC
                     HVAPHSTVIL"
     gene            9076..9627
                     /locus_tag="PLU15_03845"
     CDS             9076..9627
                     /locus_tag="PLU15_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOO30085.1"
                     /translation="MTIKIETPLSDSVVSSLRAGDEVSVSGVMYIARDAAHKRLVSLI
                     ESGEALPFDPRGQIIYYAGPTPPKPGQVIGSVGPTTSGRMDPYTIPLLELGLKGMIGK
                     GYRSAAVRKGLIEHRAVYFAAIGGAGVLAGQCMKSAKPIAYEELGPEALWRVEAEDFP
                     AFVVYDAYGGDLYEEGTSKYRVC"
     gene            9655..11298
                     /locus_tag="PLU15_03850"
     CDS             9655..11298
                     /locus_tag="PLU15_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOO30086.1"
                     /translation="MADFGYNSIYPSRRSPVMGRRGAVATSHPLAAQAGIGMLAAGGN
                     AMDAALATAAALTVLEPTSCGLGGDAFAIVWDEGKMYGLNASGKAPMSLSADRLRAAG
                     MREVPKEGWASVTTPGVVSAWSALYDRFASMPFCEIMAPAIEYAAEGAPVPPVVAGYW
                     ADSEKRFGGRKGLYAEFERVFLPGGRAPRAGEIFRNPDLAETLRSIAGSYGRSFYRGS
                     LARAIADYSQDTGGYISEEDLREHAPQWVDPVSANYRGFDVWEIPPNGQGIVALMALN
                     IIEGYELESMDLMSPEVLHVVIEALKLAFADAHGYIGDPLCVDIPTDELLSKGYADRR
                     RRLIRHDRALECPAPGNPGRAHGRWEGSDGDTVYLCTADCYGMMVSYIQSNYMGFGSG
                     IVIPGTGISMQNRGAGFSLEEGHPNELAPGRRPFHTIIPSFITRDGIPIAAFGVMGGD
                     MQPQGHVQLVSAIIDGKLDPQTAIDRPRVRVLGSQGVAAEPSIGENVADALRRLGHEV
                     RLESEPAGFGGGQMIWRDPETGVLIAGSEPRKDGCAIAL"
     gene            11320..12327
                     /locus_tag="PLU15_03855"
     CDS             11320..12327
                     /locus_tag="PLU15_03855"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOO30087.1"
                     /translation="MRRSDSWTLILGVVLMVIGSVLLLNNLGVTSVDLSYIISNFWPL
                     LIVGLGLYWVLKPDGSESGEVGEAERQATSEMPTSSVEPEPVDGEGCDECVEDAGEAD
                     ADSPRLTEVHTEESGLQEDVQSEATYDWGADTVDVADCQREGGPANGGEDEAELMRRR
                     SAMGWIIAILGLLILGSKMGRSPWSTGYAGGVFWAIVAIFIGLAMVRSSGEPAPEGKT
                     NWAVLSRIDHGQPGLELRSSSYMAILGSVVLDLSASRIPDEETRLDITAVLGCVHVVL
                     PPELSVKCEGTAVLGNVEAFGHSACGGLVNRDFVRSVPGGSSKSLHIKCRSILGSIDI
                     R"
     gene            12603..12848
                     /locus_tag="PLU15_03860"
     CDS             12603..12848
                     /locus_tag="PLU15_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOO30088.1"
                     /translation="MRSTGIVRKVDGLGRIVIPMEVRKSLEIHEDDPVSISVDGETIV
                     LKRYRPFCTFCGNTEGIRTFKGKHVCGDCLSLIRKIG"
     gene            12895..13884
                     /locus_tag="PLU15_03865"
     CDS             12895..13884
                     /locus_tag="PLU15_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30089.1"
                     /translation="MPDRDQSGYRINPVWYGAVLLIAIIITLIALSVFGRSDMHPTDH
                     DETGVSESAFCWQADLPEGWRMVPNVDGGVRFVAEGFTVGGIERLPELPQGERDLTSL
                     LSENWVILSSMQVAVAAGWGVVFDISVPVHSDESASERGFETGESQSYIAAPTRPETH
                     CLVPLDGVTLDVWTRENTGFPPKEARDAAFSILQSLGLSGEYGEMQNRLPGEVMMIAA
                     ISDEAYPDGRIWGAFDVEATVRNVSDEDASDFKVLITKAVSNPAYLESDTEVDTSLDR
                     ISSLDPKADYILRIPGLEMSLPEKDYEVTVDIIGGGRPLKLGVRMAFPPGSED"
     gene            14163..14366
                     /locus_tag="PLU15_03870"
     CDS             14163..14366
                     /locus_tag="PLU15_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30090.1"
                     /translation="MLMQSAPDRFTVHMQKDEAARLGHNMTIRGYLPQTSGAPPLIDH
                     ETSIVALGSGVGMAISSVERDTN"
     regulatory      14558..14689
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            14828..16135
                     /locus_tag="PLU15_03875"
     CDS             14828..16135
                     /locus_tag="PLU15_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO30091.1"
                     /translation="MEEYKGRQGERREYGSRRERQIALIVFAAICIIAMAFIFLWPRF
                     GSRSYVFAVVGDNQGRNEVWNVVMDGVNSDRPDFVLHCGDMVASGTRAQYDDFLLGAE
                     KLRMPFYVVPGNHDVRGEGRECFAQLVNDPPYYSFEHEGYRFIGLDASEGSIDTEQTE
                     WLRNELSKDGHKFVFMHVPLYDPRPGGDHCFLDSEQAFELEKLFRESGVLAVFSGHVH
                     MFSHEERDGVEYVTTGGAGALLYAPEEEGGFYHYVLVKVDGDQVDIEAKKVEVEFEEP
                     SLTLVGPAGEIELSLGELALMETLEVEAAFENKFENLGGKGLYVGVPVSRLVEMVGGM
                     VPGQTLVVTCADGYAQDYAYENLYPSELWAEIQGPMILAYEKDGATSPKWPESPRLVM
                     ASPDGVYSNEDCADTSMPGQGWNVYESAGARWARSVRRVEVKD"
     gene            16182..17177
                     /locus_tag="PLU15_03880"
     CDS             16182..17177
                     /locus_tag="PLU15_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30092.1"
                     /translation="MRKNRLALVVFLAFVVGFMAMPVFWMPAQAQNNWKVEVVSGQRS
                     VTLTETDFGRMKPVSAKAAFLRTTGRIEGPALYKGVPMEEILKKVGGIDQGQAIRVTS
                     RDGYAMTYSYAQVCGGILTYDNQQRALRIGGVAMYLAYESDRDSADKLPRIVFMDKEK
                     TAITDGHFWAKSVAKIEVVPGVDDWMIKLNGIEKTYFDRATFESTVNCPSTPHPGVKW
                     ETTEKDGSNSTYEGLPLWIWVAMMDGGDLPAGHYRFNDSLARKGYRVVVVSADGFSAE
                     LDSGLMARNDRIILAYKKNGQYLSKNDGPLRLVGEDLPSKKHQVKQVVEIRLEGI"
     gene            17251..18306
                     /locus_tag="PLU15_03885"
     CDS             17251..18306
                     /locus_tag="PLU15_03885"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO30093.1"
                     /translation="MNPGPMRKAYYFSTRDLVLIAAISAAGGVLSTYVGYLGNLMNKA
                     FGVPFGAGQWMAGLHVFWFVFARAIVGRTGAASLTGILKGIVEMFAGSTHGFPIVIVS
                     AVEGILVDVVLLPSGSNPSLGAMCLAGGISSASNVLIFQALYFSGVSMGYVGLITAFA
                     FASGAAFGGYFTAGVLDAVSSAHILKVDEERRSVHRPPVVRMGVAIALVLVLAGGAVY
                     YYACVFEPFWNGPTCRVEGTVAQAYNYRPIDFEDDNVTVVAELKGAVTHVPPTDYTGV
                     PLTAILERAGVGESATEIQVVASDGYYAVFDLDDVMRRQDVILAQDDETLRLIATGYE
                     GAYWVRMVSRIVVCEDD"
     gene            18299..19774
                     /locus_tag="PLU15_03890"
     CDS             18299..19774
                     /locus_tag="PLU15_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO30094.1"
                     /translation="MIDISGLRVRYYGSSELALKDIDLIVEPGESVIVTGPSGCGKST
                     LALCLAGFVPHAIGAEVAGSIRVCGINQREASVYRIAQHVGLVQQDPEGQFCTMCVED
                     EVAFGPENLRCSPYEVESRLDRALATTGVSHLRGRKLTELSGGEKQKVALAAVLAMEP
                     DLVILDEPTAYLDPRATRELIELVSRMRDADSRLTLLILEHKPWRFSHALERVIHLDR
                     GSLTYDGPIGGAAFEEEILDQLPHSHRSLHGFGEPLLRVQNLWQAYGERTVLRDVDLE
                     VCRGEIVGLMGDNGSGKTTLLHTIMGFLRPSSGRVLLEGDDITNKPVSARARRIGMIF
                     QNPNHQLFTDCVWREVVIGPLCQGDTEEECRPRADALLDSFGLSSYRDSHPLLLSYGQ
                     KRRLNLASTMASNPSLLLLDEPFAGQDVSRARDLMCILDELASEGVGVLIIGHDPDIM
                     TRCCDRILFMRDGKIQIDAPPYEAVAELERRGEHDYLPGRF"
     gene            19749..20480
                     /locus_tag="PLU15_03895"
     CDS             19749..20480
                     /locus_tag="PLU15_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO30095.1"
                     /translation="MITFPADSDGSTAVSKLNPITKLIVLVSACTVALRLKEPLHALI
                     CFGLILIGHLLTKVRPAILWQRTRGIFMFGIILFASQIALVCDVPWQTRIANGALMSL
                     RFLNVVLASHLFVSVTSSEDFAYALMQAGLPYRYGFALLAAMRFVPYFKTQANTVLQA
                     QMARGMEVDRPTPKGIFLMAKHTFVPVIVTALSKVDSLAISMEGRCFGLYSDRTYRRR
                     IPFHWWDGVVICASIAGIVASFYLI"
     gene            20586..21272
                     /locus_tag="PLU15_03900"
     CDS             20586..21272
                     /locus_tag="PLU15_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOO30096.1"
                     /translation="MKYELILFDADGTLFDYDKAERYALEQTCRQHGLGYDQPLLERY
                     RAINSSLWRQLEEGMIISSKIRVERFRRLLSEIGSDAECNWEEFAQHYLSHLAGGAWL
                     IDGAEEICRYLAPYYKLAILTNGIKEVQISRFQASPLTPYINKLIISEEVGYSKPRPE
                     IFEYAMKTLGQECRSTTMIVGDSLTSDMRGGKDFGIATCWYNPHGIENGTGLEVDYEI
                     RRLDQLKDIL"
     gene            complement(21343..22335)
                     /locus_tag="PLU15_03905"
     CDS             complement(21343..22335)
                     /locus_tag="PLU15_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOO30097.1"
                     /translation="MSDLLSPLCVKNMSLKNRIVMPPMASNTATLEGMATEATFSYYE
                     ERADRGVGLIIVEHTYVLKSGKYRERQLGIDCDDHVGPLSELTRRVHACGAAIGIQIT
                     HAGAATRSSTIGQRPLGPSVVPLSPEHEVPCEIERSFIPHLVKAFVQGAKRAVAAGFD
                     MVELHGAHGYLLNQFLSPITNIRSDEYGGSREGRLRFPLEVVTAVCSAVGPDAAIFYR
                     LGADDGVPGGLTIDDTCWAAQKLVAAGVDAIDVSGGLGGASLPGYTGEGYFAPLSEAI
                     KGAVDVPVMVTGGITTPEAADSLVRQGKADLVGVGRALLANPSWAQNAIQTLRS"
     gene            complement(22707..23555)
                     /locus_tag="PLU15_03910"
     CDS             complement(22707..23555)
                     /locus_tag="PLU15_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOO30098.1"
                     /translation="MRITILDIFWLLIIVWSFVPLLSRQTRDSHRYRVIRNFEKRRRS
                     RVITLIHRQESTNLFGMTFGRHIDIEDSEQVLRAIRLTDPNVPIDIVLHTPGGLVLAT
                     EQIAHAIKNHPAKVTVFVPHYAMSGGTLIALAADEIVMDPNAVLGPVDPQIGSYPAAS
                     ILSAVARKPVAELDDQTLILADVSEKALKQVHGLIMKILADKMPPEKAAEITDQLSTG
                     RWTHDYPITCEELKQMGLSVRTDMPREIYDLMELYPQAASRKRPSVGYIPLPYGVRGE
                     QPKQRD"
     gene            complement(23988..25064)
                     /locus_tag="PLU15_03915"
     CDS             complement(23988..25064)
                     /locus_tag="PLU15_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO30099.1"
                     /translation="MHYTVQSGDTLWLIAQRFRHDYRAIAQANSIEEPYDVRPGQVLH
                     IPRKHRLIGFVPDYAVAEGIADALASPEPFNEIIYTFIRIQPDGTVRAAALDRALQIL
                     RARRTRVLAGVTNQSDATYSREAVTATLLADDSRSHLAASIVEAVTTCCLDGVCIDFQ
                     QIDADHWAMLPKLACEIRSRFTRLSPYYTIAIVFPHDWRDHGLDLACMAQYADLMIME
                     GYEKGADSVEGPPTPVSWLEENVKAAIDKIGTGKLTVALGIYGLDWTDDEGAAYLRVE
                     AAERISRETGALIKRDQHDGTSGFVYHDASGRLHRVWYEDLMSLAVKLDTLSRLGINK
                     IAIWRLGAIPYDSLSLVAQAGMWS"
     gene            complement(25171..25713)
                     /locus_tag="PLU15_03920"
     CDS             complement(25171..25713)
                     /locus_tag="PLU15_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO30100.1"
                     /translation="MSGKPVNWITRTAILLAMTLAFQVVFKLPQAFTGPVVNMFLFLS
                     VILVGMWSGAVIGLATPLVAWMVGIMAAGPLVPVIQLGNASLCIIFGLIDETGKGKSL
                     SIRILGIAVASVIKFLFISLSAKHLLSLPEAAAKAMGSPQLFTALAGGALAVLVSEAL
                     LRTGAVTDRYGTTKKTTKFS"
     gene            complement(25735..26475)
                     /locus_tag="PLU15_03925"
     CDS             complement(25735..26475)
                     /locus_tag="PLU15_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOO30101.1"
                     /translation="MQHICNATLLGLAAGVLGTGFGAFLTAASGTPKRRTLSTLMGTA
                     AGVMLSIVFLDLIPEAIEIGGVAQTVGGFLLGVGATGIMDLLIPHFHFVSEDCKQNRF
                     LKAGLLTGIGIAMHNLPEGLAIGAGYAHGNSLGLSVALTIAIHNIPEGMAMAAPMCAG
                     GVGRKQSTLYGALAGLPMALGAFVGAAIGNISNGMLAVSLAFAGGAMVFITADELIPD
                     AQEFADGHSGTFGIVIGILAGMLLIYGY"
     gene            26606..27373
                     /locus_tag="PLU15_03930"
     CDS             26606..27373
                     /locus_tag="PLU15_03930"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOO30102.1"
                     /translation="MILAIDVGNTNTVLGVFDGEELLADYRVSTDRQRTADEWGMLLL
                     ELLDHAKIDGAAISGVIISSVVPPIITELAKMSRRYFRCDPEVVGPGMKTGISIRYEN
                     PKEVGADRIVNAVAGYELYGGPLIIVDFGTATTFCAIGQTGDYLGGAIAPGIMISAEA
                     LYMRAARLPRVELTKPQSVIGKNTVSSMQAGILYGFVGQVDEIVRRIKQELGGDPKVI
                     ATGGLAEVISLESDHIDEVNQNLTLIGLRILWERNHS"
     gene            27392..28714
                     /gene="hflX"
                     /locus_tag="PLU15_03935"
     CDS             27392..28714
                     /gene="hflX"
                     /locus_tag="PLU15_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOO30103.1"
                     /translation="MNTSFSLEGANNSSLHSGSGDNMERAVLVGLETAAPSAWNIEDS
                     IGELAHLTETAGAAVVATVIQKKGKPDPAYYVGEGKAREIEMVRSATEANLVIFDDEL
                     TPAQQRNLEEIIEGRIIDRTELILDIFAQRAATSEGKLQVELAQLNYMLPRLAGRGTE
                     MSRLGGGIGTRGPGETRLEADRRTIRNRISSITRELETVRQRRTVQRQKRRDADLPLV
                     ALVGYTNAGKSSLLNTLTGAEVLVEDKLFATLDPTTRRYTLPDGQALLLTDTVGFIQK
                     LPHHLIMAFRATMEEVVDADMLLHVVDASHPQAVEHCEAVFDVLREIDAADHPVITVL
                     NKSDLPESGPMIERLHHAYPKSVAVSAVTGDGLDDLAEMISEELSSWRVRIEELVPYG
                     DPVLGLIRARGRVISEVYREDGVAIVAEVDRMVAGQVRKARSNGSKRQ"
     gene            28764..29732
                     /gene="dusB"
                     /locus_tag="PLU15_03940"
     CDS             28764..29732
                     /gene="dusB"
                     /locus_tag="PLU15_03940"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOO30104.1"
                     /translation="MGGVRLMNPLVLAPMAGVSDAPFRRICKEMGCALVYTEMISGMA
                     LVYRNRRTLDMLHVSEDEHPIAIQLFGSDPRVMASAARIVMDGGADVIDINMGCPVPK
                     IVNNGEGSALMRDPERAWAIVAAVRQAVDVPVTVKIRKGWDDSSVNAVEFAKGCVDAG
                     ADGVAVHGRTREQGYSGDADWNIIAQVAHAVDVPVIGNGDVISAENSFEMLESTGCAG
                     VMIGRGALGNPWIFRDCLHYLATGTHLPQPQPRDRISMALRHLDDAVDLKGEKVAVRE
                     MRKHMAWYIKGLRGAARIREAINASEDAEALRSIMSAYLRQLEDGV"
     gene            29927..30469
                     /locus_tag="PLU15_03945"
     CDS             29927..30469
                     /locus_tag="PLU15_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO30105.1"
                     /translation="MTNLIRIGDKVVDRDRIDRALDKLFDMRQNGASQAEVAAALGTD
                     RSFVSRLENLGEIRKGERVAVIGFPIGNKEEIEQLAKKAGVDYVWLMNDEERWAYVED
                     RSGIELLNDVIREISDFNQYDQVIFLGSDFRVRLIEAILGEKVTSVVIGKSPIKEDVN
                     IDVAEMISIIKAVRGGAEKA"
     gene            30466..31386
                     /locus_tag="PLU15_03950"
     CDS             30466..31386
                     /locus_tag="PLU15_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOO30106.1"
                     /translation="MKHVVSVSIGSSDRNHSATVNLLGEDILVERIGTDGDMNKAVEL
                     ISELDGKVDAFGMGGIDLYMAGVGKRYVFRDAKRISQAAKKTPLVDGTILKSSLEYNA
                     VKYAAEVEKLPMAGKNVFVVCVIDRIGMGRAFEELGCNMVYGDLMFSTGMHIPLYSLN
                     SIYKLAPIFMPIMTQLPFSWLYPTGKEQNASRKRPKDKKPKFQKYYNDAHVIAGDFHY
                     IYKYLPEDISGKIVVTNTVTSKDVDELGRRGASALVTTTPEINGRSFGVNVIEAILVA
                     LIGKPVEDISDEDFTAMVQCLEIKPRIVRY"
     gene            31634..32110
                     /gene="greA"
                     /locus_tag="PLU15_03955"
     CDS             31634..32110
                     /gene="greA"
                     /locus_tag="PLU15_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOO30107.1"
                     /translation="MSDKEVILTPEGLHNLEKELEYLRTTKRRDIAARIKQAIEFGDI
                     SDNSEYEDAKNEQAFVEGRIAQLEKMLRNAKIIEGPDGDGDAVSVGSTVVLKDLNHGD
                     VEQYTIVGSAEANPSRNRISNESPVGRAVLGKTVGSIVEVNVPVGTIRYEVVQIAR"
     gene            32120..33604
                     /gene="lysS"
                     /locus_tag="PLU15_03960"
     CDS             32120..33604
                     /gene="lysS"
                     /locus_tag="PLU15_03960"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOO30108.1"
                     /translation="MDELGGQFEGAELGNELAHRKAKVEEWRSRGIDPYGSKFVRTHC
                     AAEIVEDFSELEGEEVSIAGRIMALRGHGKASFLDIVDASGRIQLYAKHDALGDELYE
                     LLGMMDIGDIIGVTGTVFRTRRGEISVAISSFRILSKALRPLPEKWHGLRDVDTRYRQ
                     RYLDLMVNPEVQRTFVLRTKIISAIREYLDSRGFLEVETPCMHAIAGGAAARPFITHH
                     NALDMELYLRIATELHLKRLIVGGFEKVYELGRIFRNEGISTKHNPEFTSIEVYEAYA
                     DYYDMMDLTEGLVAYVAETVLGTTEVEYQGDKIDFAPPWTRITMAEAVLKETGVDFLN
                     IEQTEAVAASRKLGLKVSDDATCGFCMSEAYEEFVESRLVQPTIVMDYPIEVSPLAKR
                     RADNPNLTYRFEVVCAGRELANAFTELNDPEDQLQRFSAQVAQREKGDDEAHMMDEDF
                     VTALEYGMPPTGGMGLGVDRLVMLLTDSPSIRDVILFPLMRPKE"
     gene            33622..34353
                     /gene="nagB"
                     /locus_tag="PLU15_03965"
     CDS             33622..34353
                     /gene="nagB"
                     /locus_tag="PLU15_03965"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOO30109.1"
                     /translation="MQVLVYSDYDEMSAAAARMIASKIGELPKPVLGLATGSTPIGMY
                     AELVRLHNEEGLDFSNVTTFNLDEYCGLDAEDPRSYHWFMKEHLFSHVNLDPEKAHVP
                     AGNSADPDGECARYERLIKEAGGIDLQVLGIGMNGHIGFNEPGSDPNSRTRVVRLAEQ
                     TIEVNRAKGGLNALPGYAISMGIGTIMEARQILLLANGAGKAEIIRRALTGPVTPDVP
                     ASLLQTHPNVVCMLDKPAASLLDLS"
     gene            34401..34476
                     /locus_tag="PLU15_03970"
     tRNA            34401..34476
                     /locus_tag="PLU15_03970"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34434..34436,aa:Ala,seq:cgc)
     gene            34758..34925
                     /locus_tag="PLU15_03975"
     CDS             34758..34925
                     /locus_tag="PLU15_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO30110.1"
                     /translation="MAYEINDNCIACGACKEECPTEAISEGDIYAIDPEKCIDCGTCN
                     EVCPVGAPNPA"
     gene            35199..36095
                     /gene="pdxS"
                     /locus_tag="PLU15_03980"
     CDS             35199..36095
                     /gene="pdxS"
                     /locus_tag="PLU15_03980"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOO30111.1"
                     /translation="MAIGELVNSVDRSKLEFVEKLRGGVIMDVTTPEQAKIAEDAGAV
                     AVMALERVPADIRAQGGVARMADPALIEQIMDSVSIPVMAKVRIGHFVEAQILEAIGV
                     DCIDESEVLTPADERYHVDKHAFTTPFVCGCRNLGEALRRIGEGASMMRTKGEAGTGD
                     IVEAVRHIRAVNEDIRRLKNMPCEEIMAMAKEIGAPYELLCQVARDGKLPVVNFAAGG
                     VATPADAALMMQLGADGVFVGSGIFKSDDPAARARAIVGAVANYRDAKALAEVSKGLG
                     EAMKGIDVSSMADEEKLQGRGW"
     gene            36189..37448
                     /gene="glyA"
                     /locus_tag="PLU15_03985"
     CDS             36189..37448
                     /gene="glyA"
                     /locus_tag="PLU15_03985"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO30112.1"
                     /translation="MYDNIRREDPEVADAISNEIRRQNEKIELIASENFVSEAVLEAL
                     GSPLTNKYAEGYPGRRYYGGCEHVDVVESLAIERAKRLFGAEHANVQPHSGAQANMAV
                     YFAALEPGDTVLGMNLTHGGHLTHGMHLNISGKYFHFIPYGVREDTQIIDYDAMENLA
                     IEHRPKMIVAGASAYPRIIDFERISEIAHSVDALLMVDMAHIAGLVAAGLHPNPVEYA
                     DFVTTTTHKTLRGPRGGMILCKSEWARKIDSAVFPGTQGGPLMHVIAAKAVAFAEALQ
                     PEFAQYQRGIIMNAQALADALMERGWRLVSGGTDNHLILMDLRGTGITGRVAEEALDE
                     VGITVNKNTVPFDPEKPMVTSGVRIGTPAVTTRGMKEPEMREIASLIDKTLRNLGSEG
                     VMDEVRQGVNDIISRFPLYSRIRAELS"
     gene            37602..38177
                     /locus_tag="PLU15_03990"
     CDS             37602..38177
                     /locus_tag="PLU15_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO30113.1"
                     /translation="MAKLLEIRWHGRAGQGAKTAALVLAEAAISTGKYAQAFPEYGPE
                     RMGAPMKAYNRISDEPITVHHHVEEPDIVIVLDPTLLTVGNVKAGVPDDGIYLVNTPL
                     SPSEMRTRLGLEGSKAKVYTLDATQVSMDTIGRNIPNTPMIGALIAATGLLELDELLE
                     DTRRKLSKKLRPEVVDKNMEAIKRAYREVAS"
     gene            38177..38482
                     /locus_tag="PLU15_03995"
     CDS             38177..38482
                     /locus_tag="PLU15_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO30114.1"
                     /translation="MSVPKDAGWKDVPKAGRILEPGCADKYETGGWRTYRPVHIEENC
                     INCLFCWIYCPDQAILVEDGKFKGIDYAYCKGCGICAKECPGKRGNKALKMVLDEEA"
     gene            38509..39690
                     /gene="porA"
                     /locus_tag="PLU15_04000"
     CDS             38509..39690
                     /gene="porA"
                     /locus_tag="PLU15_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO30115.1"
                     /translation="MSIRVGKTGNEAVAEAMRQINPDVVAAYPITPQTEIVQIFSSFV
                     ANGKVDTEFVTVESEHSAMSATVGASAAGARAMTATSANGLAFMWEVVYIAAAMRLPI
                     VMPVVNRALSGPLNIHCDHSDTMGARDSGWIQIFSENAQEAYDNAIQALRIAENSNVL
                     LPVMVTMDGFIISHAMEVMEILEDEKVREFIGEYVPENPLLDVDNPVTIGPIDLQDWN
                     FEHKRQQAEAMRNAKPVILEVAKDYAKLTGREYGLFEEYRMEDAEVAVVVLGSTAGTA
                     KAVVDELRADGVRAGLLKIRVFRPFPAEEIAEALKDVKAVAVMDRSDSFNAVGGPVFG
                     DVRSALYDVENGPRIVDYIYGIGGRDVTPDHIRTVYADLAKIADTGQVENLLTYLGVR
                     E"
     gene            39702..40628
                     /locus_tag="PLU15_04005"
     CDS             39702..40628
                     /locus_tag="PLU15_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO30116.1"
                     /translation="MATLKELSKKEVLISSGHRACAGCGFPVIIKQALLAAENPVVVS
                     CATGCMEVCTTIYPYTAWKVPFIHNAFENSASTLSGVEAAYKSLSRQGKIDKKIDFIA
                     FGGDGGTYDIGFQALSGAMERGHNMLYICYDNQAYMNTGIQRSSATPRGANTTTSPAG
                     TESYGKKQYRKDLSAIMVAHEIPYVAQASPSNWNDLVSKVKKALAVEGPAFMNIIQPC
                     VLGWKYPSDQSMELARLAVDTCFWPLYEVEDGEYRLTYKPKEKKPVEDWLKRQDRFRH
                     LFKPENAEIVKEIQEDVDERWAKLLAKCGEEA"
BASE COUNT         9507 a         9646 c        12374 g         9772 t
ORIGIN      
        1 ggaaattgac cagcatgatt ccgagccgta ttttcggacg cttggcgggc caggacagac
       61 tcaaatggct gaaatggtcg tcagtttgaa cagtagagct ggagcgaagg ctattggcga
      121 tgacaattgg gcgtgcaata acacgctatg tgagaaacac ggcctggctg ggcaagctat
      181 ggataggatg gggtttctga cttggtggag gggtcaaatg gccctgattt aagtgaggtc
      241 agaacatttt cagatattgc gtttgcgctg aagatcgata cgcctctggc gcccgccgcg
      301 cgagctgctt ctacgagggc aggcacgcct tcgcccgctt gggaatgggt catcgaatct
      361 gcatcaatcc ccgcgatcat ttgcgtcttg ttcccggcga aggattgggc cgcccgtatc
      421 atcgtgtcga acagatcgag gtcttgcgtg taggtgaaga cgactacaaa atcgaggcac
      481 cctttctcac accaagcttt ccagtcctgc atccttgtga ggattgcttc aggataggga
      541 tagacggcgg cggagaccag agttcctgga gcagcagacc taattgcatc ggagagcttt
      601 atcatgaaac tggtaagatt gttgcaccgc cagtcgaacc aagcgttcca tttcgctatg
      661 tcgagcctta cgtcacgggg ttgaaagccc aggtcttccg tagccagttt cgtgatggtg
      721 ggtgcatagt cgtacggaat ggttcggccg tacggatagc ggaggtagtc aaagtgaaca
      781 ccgtcgacgg cgtgttctgt gaccaattcc cacgcaaggt cgagtatgaa ctcctgaact
      841 tgggggtgag ctggggacag aaagcaccta tctgattcgg ctgctgaagg aatgcggcca
      901 tcaagcccga cggccgccca atccgggttt tcctgaagga tctgcggcgg cgtttccttc
      961 tctgacgcga atccgacaca gaaggtctca agccaggcgt ggatctgaat accgtgtttg
     1021 tggccttcgt ctatggctat tgcaaggggg tcaaattccg aataaatggg gttttgcacg
     1081 ccgacatcgc ttgggtagat tgtgtagcca tcatagaaag agtccacaaa gacgaggttg
     1141 aaattggcgt tggcgagccg cttcacatcc gcccgtatct gttcttctct ggccgcaggt
     1201 cgataccacg ttgctctggt gtctagccgc cctaggttga acttggcctt gtatttcgtg
     1261 ttgacgaggt aatcgatggt attggttatt aatgtgacta cctcgggaga tgaaaggttc
     1321 ttgcgagcaa aaagattctc tgaacaataa atgccatatt cggtttcgac tatcgcagat
     1381 cggcctgttg accacttggc caggatgatc gcgttcggat tcatcttatt ggtgagccca
     1441 taagtgcttg gcacaggtat atctgattca atgcctcgaa aaatagggtg cgatttggca
     1501 gcggggacta tgtggttgtt ccctgaagtg ccagtcgatg aatatcgtac gcccataatt
     1561 gagctgagtc catagtcagt acgggtccgc caaaaatcgt cgcgaaggga tgtgtcgtat
     1621 gtagaaaata ctcggcctcc cgccgtcgca aaagcctcta tttggtcgat tgcaagagaa
     1681 gacatgcagc tggttgcagg caggatcaaa gctttgtatg acgacagagc gcctatttcg
     1741 acatcatggt cattgattac ggctgccgaa atgcccgacc gtgccaatgt actcattagc
     1801 catgtagcgt caatgaactc ctcgctatat gtccacgctt taagatccgc gtaatgcctc
     1861 gtatatcccg aatcgaggat tgcgacctcg gcgccttcga accccaaccc gggagttgaa
     1921 acgttagcgg tagcgccaaa agcaggattg aagcctgcga tcaatatgaa tgccaaggat
     1981 acaataaccg cggcagagcg atatctcaaa gtgaattcac ctcattttgg ccttggaaag
     2041 tcccaattta aatcataatt cttcgtaagg ggcaggtttt cctccagcaa tacgcattcg
     2101 cacatggctt ccatatctgt atggggttta ctgagattgg ctgaaactac cacagttgtg
     2161 ccaaggcgcg gactttgcct ttaagcctac taacccaatt ggagaaattc gggccattgg
     2221 gtgtaaactt gagtttgggg tgattagcgg tggcagctgg ctgccgaagc ggcgcgaagt
     2281 gccgaacaca tggaggagac tgacgcgatt gtgtcgaatg gttgattgct gtccgggagc
     2341 aaatctaggg aaacgctcgt cttcgctcag ctgtagagcg tttgcgcctc agagattggg
     2401 ttgatgcaca tcgaagtatt tgtcgggggt gcaatctcag caattctgat tccagtgttc
     2461 cggtattagg gatatccaat tcgccccgag ccatgcatat gtaggggtgt tgcttccatc
     2521 acatgagatc tggttagtca atgatattga cccgatgcca cgatgccgtt ttatgggtgt
     2581 tgacagctgg gcgtgctttc ggatattacc ggagacagca tctatctgga tgtcgcgcgc
     2641 ggggtggtca attcatggat tgacaaggtg ctcattcgga ggcaagatgg tgttatggac
     2701 tggaactggg attatggtgg caaggtcggt aaactgaact ggaccgccga ggagtttgac
     2761 aagacacaag gggcatttgt ctccggattt tgggcgacaa gagatgggca cgaatgggtt
     2821 tgcagtgtgc ggaggctccg ctctgcgccg gcgttgccaa gcctcttaac gctcttttag
     2881 aatttgtgag gctgccgagg gttgtggtgc agacattgat tgcatcctct ggggatctcg
     2941 tcgacagtct gctatcgctc aaacaggcat ccaggtatat ttcaccaaac ccgctgccta
     3001 tggcacgcat cgggtcgtag ccgccacggc agacgcttac aaggtaaccg ggcggtacaa
     3061 gtgcttggtg tatgctattg ccagggccaa ttggctaata ggccagacgg aaaccgagtt
     3121 tcaaggatac ggaagcaatg tatgatctgt gatatggact cttgaagtta tggaagcagt
     3181 catcagtgct gacgaggcta tgagcgcgga caagtagcgc tcaaatcaaa cagttgcaaa
     3241 tctcaggagg gaattctagg gatgaacaga atccgaaagt cgtcaatgct cctaagtatc
     3301 gttttgctct tggcattaat gatgggaata ggcatcccca tggaggctgc ggccaagcct
     3361 cgtgtggcta ttgtttttgc tacaggcgga ttgggtgaca agtcgtttaa tgactctgct
     3421 tacgacggca tgaagcgagc agaaaaagac ttgggaatcg aattcgataa agttgaacct
     3481 caagctgtag ctgagtatga gacattgttg acgcgttttg cgcagactcg caagtatgat
     3541 ctgatcatat cgatcggctt cgatcaggct gatgctctga aagttgtatc agcgaggttc
     3601 ccaaatcaga aattcgccat tatcgacaca gcagtggacg ctcccaatgt agcgtcgtat
     3661 gtatacaagg aggccgagcg gggatttgtg gttggcgcca tagcaggtct gatgaccacg
     3721 aaaaaaggcg acccgcgcat tgtttctgac aaacaggtgg tcggagtcat cggtggcatg
     3781 gacatcccgc ttatccgggc caacgtcgcg ggctacattg ccggtgccaa gtatgtgaac
     3841 cccaaggttc aggtgaagta cgcatacgta ggcgattggg ccgatccggc caagtgcaaa
     3901 gagatggcaa acgccatgtt tgacgagggc gtcgatgttc tgtggcttgc agccgggcgt
     3961 tctggccttg gtggcatcaa agcggcagag gagcggaatc gctacgcaat aggcgctgac
     4021 tcggatcagg gctatcttgc tccccgcaat atactgacca atggaatgaa actcgtggac
     4081 cgaacagtgt atatagccat caatgacgtt ctgcgtggca agttcaagcc tggagtcaat
     4141 atgctcggac tcaaagaagg ggcacttggt tactccaaga gcctggttcc tgcagatgtc
     4201 atgtcccagg ttgacaagct tgcggccaaa gttgtatccg gcgatatcaa tgtgcctgat
     4261 gagatcgaca aggttgagcc ctggctcaag gcccaaggca agtagcaagc tgtcagcggg
     4321 ttgaattaat atgtaagcgc aatgggcggg gggataatct ccccctgcct tgccaggttt
     4381 aactcggaaa aggggttcac cttacgatgt atgctgttcg aatggagaac ataacgaaac
     4441 gctttggaag cgttaccgcc aatgaagcag ttgattttgc tgcgtttccc ggcgagatcc
     4501 attgccttct gggcgagaac ggggccggga aaagcacgct catgaggatt ttgtttgggc
     4561 tttgccgcgc ggattcaggc ctcatctgca tagacgacga gccggtagac atatccggtc
     4621 ctcgtgatgc aatttcgttg gggataggca tggtgcatca gcatttcatg ctcgctgaac
     4681 gtcttactgt agaggaaaac ataattgccg gatgtgaacc gagcaaaaat ggtttggttg
     4741 attatgatgg ggcacgcgaa cgcatcctgg aggtttcgca gcgttatggc ctcacagttg
     4801 atccaaccgc tgtggtcgag gatatctccg tcggccaaca gcagcgggtg gagataatca
     4861 aggctctgat gagaggagca aaggtcctta tcctggacga accgactgcg gtcctcactc
     4921 cgcctgaggt ggaggagttg ttcgttgtga tgcgggcttt gagggatgcg gggacgacga
     4981 taatattcat cacgcacaag ctcaaggaga ctatggagat ttccgacaga gtgacagtgc
     5041 tccgcgatgg caggacagtt ggtgtagtga atacggctga tgctacacca gacagccttg
     5101 tagagatgat ggtggggcgc agccttccca tacatgacag ccatcgcgtg ccggctaaag
     5161 acgggatttt cctccaactg aaggatgtgt cggtagccgg aaaaaagggc agccgggcaa
     5221 ggctcgacag tgtgaacatc gctgtgagag gcggcgagat actgggcata gcgggcgtcg
     5281 acggcaatgg ccagctggaa ctggaggaag tagcaactgg gttgcagcgg ccgagcaagg
     5341 gtcagatcat cgtcaatggt caggatgtga ctggctccga tactaggagc atcagggata
     5401 gcggtgttgc ctacatccca tctgaccgat tgaagcgggg catggtggac tccttcagca
     5461 ttgcgaggaa ttccgtgctc ggaatgcatc atagtgccca tttcagtcat cgcggggtaa
     5521 taaaagagga cgttgtgcga cggcatgcgg agaatcttgt tgatgtttat gacgtgcgat
     5581 ctgaatcgat cgatcagccc atcggttcgc tgtcaggggg gaaccagcag aaattggtgg
     5641 ttggaaggga gctgcttcgg cctcacagtg tcgtggttgc cgcccaacct actcgcggat
     5701 tggatgttgg agcaacccac tatataaggg agcagatact tgccagccgc aacaagggca
     5761 aagcagttct cctcatttcc gcggagcttg acgagattct tgagctgtct gataggatag
     5821 cggtcatata cgaaggccga atagtggacg tacgtcctac aagccagtat accgagagag
     5881 agctaggcct tctcatgaca gggcagagga acggggtgtg accgatggac atttcaggtg
     5941 atgcgtcaaa caagttgtcg aaaaagaacg cagaatggtt tgcccgtccc gggtttgcca
     6001 gattcgtgta ttcagcagct gcagtcgtgc ttgcgctgct aataggggct gtcttgatag
     6061 gtgtctcagg ctatagtgtg aaagaggcat attacaacct cttcatggga gcgtttggag
     6121 gcgtatacag cctcgcaact accatagtaa tggcgactcc gctcctgttc accggacttg
     6181 cagtagcatt tgcatttcag tgcggcttgt tcaacgtggg cgctgaaggc caactctatg
     6241 tgggcgctct ggcatctgcc gtagtccctc tggctcttga ggggctgccg cctgtgctga
     6301 gggtgatcat agctctcctg gcgggggcag gcgcaggggc ggcgtttggc tttctgcccg
     6361 gatatctcaa agcaaagata ggggcccatg aagtcatcac cacaatcatg cttaattaca
     6421 ttgccacatt actcaccact tttctggtga aaacgtactt cagagacccg ggcccggttg
     6481 atcagacagt cctgttgccc cttggttcta gattgcctga gatcatacct ggaaccagag
     6541 tctcgctggg catagtaata ggaattcttg ctgttgcgct tctcaattac gtaatacgca
     6601 ggactgtgct tggatatgag atacaggctg ttgggcagaa tcctcacgct gccgagtacg
     6661 gcggggtgaa tgtcagagca agaatggttc aagctatgat gatcagcggg gctgtagcag
     6721 gccttgccgg ctcagtggag gccatgggga ctctgtatcg gttcatacca tacttttcac
     6781 ccggatacgg ctttactggg atggcgatag cggtgattgc cggcgagagc ctctgggctg
     6841 tgattcccgc ggcgatcctt ttcggagcgc ttcaatcagg cggccagtcg atgcagcttt
     6901 ttgcggggat acccatggag ttgatgacga tggtgcaagg actggtgata ctgtttgtag
     6961 ctgcgccagg catggcggaa atgatatttc ggcggaagag ttgtggcaag gaaggcggtg
     7021 gccgggcatg agcggatttg tcgacatagt gttcaatgtg aacaacctgg cggctaccat
     7081 aaggatggca acgccaatag ccttggctgc gatggggggc gccttctctg agagatccgg
     7141 agtggtgaat atcgggattg aaggcatgat gctcacaggc gcgttctgtg gtgtgctcgg
     7201 gtcccacctt acgggaaacg catggctggg agtgttgttt gcgattgtcg gcggcggact
     7261 tgtcggcgcc ctccttgcgg tatttgccat ccggctcaag gcggatcacg ttgtaaccgg
     7321 ggtgggaata aacatcttgg cctcgggcgc cacaacatgg ctgatgcagg tgatatggaa
     7381 gcgcaaaggc gcatccgatc tcgttgctgg tctcggaacc tggtcagtgc ctgggctgtg
     7441 cgatgtcccg gttctcggga cgattctcgg tactcattcg cctctggtgt acatcagttt
     7501 tgtgctgatt tttctaggtt gggttttgat gttcaagacg cctttggggc ttcgcatcag
     7561 gatgacaggc gagcacccgg aggcagccga tacagtgggc atcaacgtcc atgcaataca
     7621 gtatttttgc gtgattctca gtggatgctt ggcagggctg ggaggagcat atctctccct
     7681 tgctcagctc aattggttta gcaaagacat gtcggctggg cgcggttaca tggccctggc
     7741 ggcatgcata ttcggcggtt ggaatcccct cgggggcttg ggcgccagct tcctgttttc
     7801 atttacagac gccatacaga tgcgaatgca gacagctcag atatcgctgg ctgtcgagat
     7861 aatccagatg atcccttaca tcttgactat tctgattctt gccagtgcag tagttcgctc
     7921 taggcctccg gcggctcttg gcaagcacta cgagtctggg catgcaaaca agtgactttg
     7981 aagtcatgag ttagctgtgt catggtgctt tgttgcgcct gttgcgggct gactgcggct
     8041 ttattgccgg gtcagcccgt cgctatttac caccatcttt ttggaaatgg gcgccggggc
     8101 gccgaaggaa tggatgattt gagcgagaag aataggaaag aagacaggca agacgtgaat
     8161 ggagcgaacc ctgattgggg aggaggattc ggtttgagtt gcgcagacgt cagacatatc
     8221 gatgcatctg aaattgcgga ggcagtatac aagctgtgca tgaaggttac atgcgtgctc
     8281 cctccggaga atatcgctgc tttggagagg gctatggagg aagaaaaatc gcctcaaggc
     8341 aggcaggcgc ttgccagaat actcgaaaac cacagactgg ccggcgagct aatgcaacct
     8401 gcttgtcagg acactggcat tgccgtagtc ttcatcgagc ttgggcagga tgtgcacgtg
     8461 tgtgggggcg acctaaacca ggcgatcgac gaaggagtca gacgcggata ttcggaggca
     8521 tatctcagaa aatcggtggt gcacgacccc ctgtttggca gagtcaatac taaggacaat
     8581 acccctgcca tagtccatct tcagatggtc ccgggatgct ccctcaagat taccgtagtt
     8641 gccaagggtg gcggggctga gaacatgagc ggacttgcca tgcttactcc ggcatctggc
     8701 gtagaaggtg tccgcaattt cgttgtggac caggttcgga aagccggccc taatgcatgt
     8761 cctcctgtga ttgtaggagt tggtgtaggc ggcaactttg agacagtggc atttctggcc
     8821 aagaaggctt tgattcggcc cgtaggggaa cggaatccga atccaaagta tgcagcattg
     8881 gaagaggaat tgttgcgcga gataaacggg ctggggattg ggccgcaagg atttggagga
     8941 tccgttaccg cccttgcagt ccatatagag gcgtaccctt gccacatagc aagccttcct
     9001 gtagcggtca atctcaactg tcacgtggcg ccgcactcca cagtaatact gtaatttgcc
     9061 ggagggatta attagatgac cattaaaatt gagactccgc tttcggatag tgtcgtctcc
     9121 tctctcaggg caggggacga ggttagcgta agcggagtta tgtacattgc tcgcgatgca
     9181 gcgcacaaac gtttggtttc gctcatcgag tcaggcgagg ctctcccatt tgatcctaga
     9241 ggccagataa tttactatgc aggccctacg ccgcccaaac ctgggcaggt gataggttct
     9301 gtggggccta ctacgagcgg acgtatggac ccatacacca ttcccttgct ggagctgggc
     9361 ctaaaaggca tgatagggaa gggataccgc tccgctgctg ttcgcaaggg tctaatagag
     9421 catcgtgcag tctattttgc tgcaatagga ggagcagggg tacttgcagg gcagtgcatg
     9481 aagtctgcca aacccatagc ctacgaggaa cttggccctg aggctctgtg gcgcgtggag
     9541 gcggaggact ttcctgcatt cgttgtctac gacgcatatg gcggggacct ctatgaagaa
     9601 gggactagca aatatcgggt gtgctagcgc tgcctcgatg aagggggata caggatggct
     9661 gatttcggct ataattcgat ttatccgtct cggcggagcc ctgtaatggg acgtcgcgga
     9721 gcggtggcca cgagccatcc attggctgct caggcaggca tcggaatgct tgccgcaggg
     9781 ggcaacgcta tggatgcggc tttggcgacg gcagcggctt tgacagttct tgagcctaca
     9841 tcgtgcggac tgggcgggga tgcatttgct atagtctggg atgaagggaa gatgtacggg
     9901 ctgaacgcgt ccggcaaggc cccgatgtct ctttcagcag accggcttcg agcggctgga
     9961 atgagagagg tgcccaagga gggttgggca tctgtcacta cgcctggcgt ggtttcagcg
    10021 tggagtgcgc tgtatgatcg ctttgcttcc atgccatttt gcgaaatcat ggcgccggcc
    10081 attgaatacg ccgctgaagg cgcgcctgtc ccacctgtcg tggcaggtta ctgggcagat
    10141 tccgaaaagc gatttggcgg gcgcaaagga ctttacgccg agttcgaaag ggtttttctg
    10201 ccgggcgggc gagcgccccg tgcgggggag atcttcagaa accccgatct ggcagagaca
    10261 ttaagatcga ttgcaggttc atacgggcga tccttttaca ggggcagctt ggcgcgggcg
    10321 attgccgatt actcgcagga tactggaggc tacatatctg aagaggattt gcgtgaacat
    10381 gctccacaat gggttgatcc cgtgtccgca aactaccggg gattcgatgt atgggagatt
    10441 cctcccaatg gccaaggcat tgtggcactg atggctctca acatcattga aggttatgaa
    10501 ttggaatcta tggacctgat gtctcctgaa gtgcttcatg ttgtcattga agccctgaag
    10561 ctggcttttg ctgatgccca tggctatata ggtgatccgc tttgcgtgga tattcccaca
    10621 gatgaactgc tatcaaaagg atatgctgac cgcagacgga gactgatccg tcatgatagg
    10681 gcgcttgaat gtccagcccc tggcaatccc ggaagagctc atggacgatg ggagggttca
    10741 gatggggata cagtctatct ttgcacagcg gattgttacg ggatgatggt ctcttacata
    10801 caatcaaatt acatggggtt tggctccggc attgtaattc cgggaactgg aatctctatg
    10861 cagaatcgcg gagctggttt ctcattggaa gaagggcatc ccaatgagct tgctcctggc
    10921 aggaggccat ttcacaccat aatcccgtcc tttataactc gagatggtat tcctattgct
    10981 gcttttggag tgatgggggg agacatgcag cctcaaggac atgtccagct agtttctgct
    11041 atcatcgatg gcaagcttga cccgcagacg gccatagacc gcccccgcgt gcgcgttctg
    11101 ggcagtcaag gtgtagccgc agagccgagc atcggtgaga atgttgcgga tgcgttacgt
    11161 cgcttggggc atgaggttag gcttgagagt gagcctgctg gattcggcgg tggccagatg
    11221 atctggcgtg accccgaaac aggggtattg atcgcaggga gcgaaccgcg aaaggatgga
    11281 tgcgcgattg cgctatgatc gggaatgggg gggcgtgaaa tgagaagatc tgattcatgg
    11341 acgcttattc tcggagtcgt gctcatggtt attggatctg tgctcttgct caataacttg
    11401 ggcgttacca gtgttgatct tagctacatt atcagcaatt tctggccttt gctcattgtt
    11461 ggcctgggac tatactgggt gttgaagcct gatggaagcg aatctggcga ggttggcgag
    11521 gcagagaggc aggcaacatc ggagatgcct acctcgtcgg tagaacctga gcctgtggac
    11581 ggagagggat gtgatgaatg cgttgaagat gctggcgagg ctgatgctga ctccccgcgc
    11641 ctgacagagg tgcatactga ggagagtgga cttcaagaag atgtgcagag cgaagctacg
    11701 tatgactggg gcgcagatac ggtcgacgtt gctgattgcc agcgagaagg tgggcctgca
    11761 aatggaggcg aggatgaggc tgagctgatg aggcgtcgtt ccgccatggg atggatcata
    11821 gccatactcg gcttgctcat cttaggcagc aaaatgggac gttctccctg gagcacgggg
    11881 tatgccggcg gagtgttctg ggcgattgtt gccatattca tcggcctggc aatggtgaga
    11941 tcatcgggtg aacctgctcc tgaaggcaag acaaactggg cggtgttgag cagaatcgat
    12001 catgggcaac caggtttgga gctgcggagc agttcgtaca tggcgatcct tggcagtgtt
    12061 gtgctggatt tgtcggcttc acggattcct gacgaagaaa cacgcctcga catcacagca
    12121 gttctcggct gcgtacatgt ggtgctcccg cctgagctgt ctgtcaaatg tgaaggaacg
    12181 gctgttttgg gcaatgtgga ggcatttgga cattctgcct gcggtggatt ggtcaaccgt
    12241 gatttcgtgc gcagtgttcc gggcggttcg agcaaatcgc ttcatatcaa gtgccgttca
    12301 atactgggtt ctatagatat ccgataacgc cctattgtag ggctcttgtg ctgacagccg
    12361 gagaacgatg ggggcctgga attgttgtga cgaggtccgg ggtttgagat atcgacgccg
    12421 ccgctaaatt gcgatggact gttgcatgtt ttctcatcct ctcgggagga gtcacgagaa
    12481 aattagcgaa attattcaag atggggtatg tgcatgcgta aagacgcggc gaacccaatg
    12541 atccaggctt tgtcgacctg cgcgcatatg gcgcaagcta gccagtatag gggggattac
    12601 aattgcgttc tacaggcatt gtaagaaagg ttgatgggtt ggggcgaatc gtgataccta
    12661 tggaagtccg aaagtcgctg gagattcacg aagatgaccc tgtgtccatc agtgttgatg
    12721 gggagaccat agtgctcaag aggtacaggc ccttctgcac gttctgcggc aatactgagg
    12781 gaataaggac gttcaagggg aagcatgtgt gtggcgattg cctatcattg ataaggaaga
    12841 taggctagcg gagtttagca catggcgtag aatggtcatg aagggaggat ctttatgcca
    12901 gatcgtgatc agagcggata tcgaattaat ccagtctggt acggcgcagt tttgctcatt
    12961 gcaatcataa ttaccttaat tgcgctgtcg gtgttcggac gttcggacat gcatccgacg
    13021 gatcacgatg agactggcgt ttctgaatct gcgttctgtt ggcaagcgga tcttcccgaa
    13081 ggttggcgga tggtcccgaa cgtggatgga ggagtaaggt ttgtagcgga aggattcaca
    13141 gtgggtggaa tagagaggct cccagagctg cctcagggag aacgggacct tacgagcttg
    13201 ctctctgaaa actgggtgat actctcctcc atgcaggtgg ctgttgcggc cggatgggga
    13261 gttgttttcg atatatcagt gccggtgcac tcagatgaat cagcttctga gcgcggcttt
    13321 gaaactgggg aatctcaatc gtacatagcc gcgcctacca ggcctgaaac ccactgcctt
    13381 gttccgctcg atggtgtcac gctagatgta tggaccaggg agaacaccgg ctttcctcct
    13441 aaagaggcca gagatgccgc cttttccatt ctgcaaagct taggactaag tggagaatat
    13501 ggcgagatgc agaatcgcct ccccggagaa gtgatgatga tcgccgccat atcggatgaa
    13561 gcgtacccag atggacggat ctggggagcc tttgatgtgg aggcaacagt tcgcaatgta
    13621 tctgatgaag atgcaagcga ttttaaggtg ttgataacaa aggcagtctc gaatcctgct
    13681 tatctcgagt cagacacgga ggttgacact agcctggaca ggatctcttc tcttgacccg
    13741 aaagcagatt acatcctgag aattccgggt ctggagatga gtcttccgga gaaggattac
    13801 gaggtgactg tcgatatcat cggcggaggg cgcccactca agctcggggt gcgtatggct
    13861 tttccgccgg gttcagaaga ctgaatagcc ttcgatcagc actccagttt caaactagca
    13921 agatccagct ttgcggtaac gcgagtttca atatggtgca ggcgatagac agggggcacc
    13981 tggaaaggcg gacgcatttt ccgacgtgat ctgcgccact acttcagact gaaaaaggtc
    14041 gcccgctcaa gtaaggtgag gcagcaatct tgttcgagcg ggcgcgttct actgcgtgga
    14101 ccttccaatc cagagctcat cacatcaatg acggccacgt gttaagatgg ctgcggctac
    14161 ctatgctgat gcaatctgcg ccagaccgtt ttacggtcca catgcagaaa gacgaagctg
    14221 ctaggctggg ccacaacatg accatccggg ggtatctgcc tcagacttcg ggtgcgccac
    14281 ctcttattga ccacgagaca tccattgttg ccttgggatc tggtgtaggc atggcgatca
    14341 gttcggttga gcgcgacacc aattaggaaa attcatttgt ggcccgacaa tggcattgtt
    14401 gcttagaaga agtctgtgaa ggcctatggc gcagggcatt tcattgaagg gcttttgcag
    14461 ggtgcgtgtt tttctatcca tgaaggcaga agtgaatcga tgcagaggca agaagcgttg
    14521 cgattgtagg gacgtgtggt tacgccgtcg aatagtcaat gagattgctt gtccgagccc
    14581 acaggccttg ggctcaggcc tgaaacggag ctgcggcgtg tgggccgata gggcgtatct
    14641 ggaaatgggt gtgcttcccg atttggaaag gagagcaaac accggattct cgggccgggg
    14701 tctgccacct ttctgtggcg cccggcccat tctgtcataa actatcgaca tttgcctgct
    14761 gcagttccag gagacttttg gggcattgca aggcccctac agaacggctg taagcggagg
    14821 tgggaaggtg gaggaataca aaggacggca aggtgagcgc agagaatatg ggagccgtag
    14881 ggagagacag atcgcactca tcgtattcgc cgccatctgc attatagcta tggcattcat
    14941 tttcttgtgg cctcggtttg gtagccgaag ctatgtcttt gccgtcgtcg gtgacaacca
    15001 ggggcggaac gaagtctgga atgtcgtgat ggacggggta aactccgacc gtcctgattt
    15061 cgtgcttcat tgtggcgaca tggttgcatc gggcacaagg gctcagtacg atgatttctt
    15121 gctcggtgcg gagaagctca gaatgccttt ttacgttgtg cctggaaatc acgatgttcg
    15181 cggagaagga cgagagtgct ttgctcagct cgtcaacgat ccgccgtact actcgttcga
    15241 acatgaaggg tacaggttca taggcttaga tgcctccgag gggagcatcg acacggagca
    15301 aacggagtgg ctcaggaacg agctgtccaa ggatggccac aaattcgtct tcatgcatgt
    15361 gccgctgtat gaccctcgtc ctggaggaga tcactgcttc ctcgattccg agcaggcgtt
    15421 cgagctggag aagctgttca gagagagcgg tgttttggcc gtgttcagtg ggcatgtcca
    15481 catgttcagc catgaagagc gcgatggggt cgagtatgtg acaacaggcg gcgcgggagc
    15541 tctcttatac gccccggaag aagagggcgg attctaccac tatgtcttgg tcaaggttga
    15601 cggtgaccag gtggatatag aagccaagaa agtggaagtg gagtttgaag agccttcttt
    15661 gacgttggta ggccccgctg gggagatcga gttgagcctc ggcgagctgg cgctgatgga
    15721 aaccttggag gttgaggccg ctttcgagaa caaattcgag aaccttggtg ggaagggcct
    15781 ttacgtgggg gttcctgtaa gccgtctcgt cgaaatggtc ggggggatgg ttccaggaca
    15841 gaccctcgtg gtgacatgtg ctgatggata tgctcaggat tatgcatacg agaatctcta
    15901 cccttctgag ctgtgggcag agatacaggg cccgatgatt ttggcttacg aaaaggacgg
    15961 cgccacttcg ccaaagtggc cggagtctcc gaggcttgtt atggcgtctc cggacggggt
    16021 atatagcaat gaggactgtg cagacacatc catgcccggg caggggtgga atgtgtatga
    16081 gtctgccggc gccagatggg cgcggagtgt aagacgtgtt gaggtcaagg attagccttc
    16141 gtttgcgtgt gttggtaacc gaaaatggag gaggttattg tatgaggaag aaccgtctag
    16201 cgttggtcgt gtttctcgct tttgtggtcg ggtttatggc gatgcccgtg ttctggatgc
    16261 ctgcacaggc ccagaacaac tggaaggtcg aagtagtatc gggacagaga agcgtcactt
    16321 tgacagagac cgattttggc aggatgaaac ctgtgtcggc taaagctgct ttccttagaa
    16381 ctacagggcg aatagagggg cctgcgcttt acaaaggcgt accgatggaa gaaatactca
    16441 agaaggttgg aggcatcgat caaggccaag ccattcgcgt cacatctcgt gacgggtacg
    16501 cgatgactta cagctatgct caggtttgcg gaggaattct cacatatgac aatcagcaaa
    16561 gggctttgcg catcggcgga gttgccatgt atttggccta tgagtcggat cgggactccg
    16621 ctgacaaact cccgagaata gtctttatgg acaaggagaa aaccgcgatc actgacggac
    16681 atttctgggc gaaatcggtg gccaagatag aagtcgttcc cggggtggac gattggatga
    16741 ttaagcttaa tggaatagag aagacgtatt ttgaccgagc cactttcgag agcaccgtca
    16801 attgtcctag cactcctcat ccgggtgtca agtgggaaac aactgaaaaa gacggtagca
    16861 attcgacgta cgagggactt ccgctctgga tctgggtggc catgatggac ggaggggact
    16921 tgcctgccgg gcattaccgc ttcaatgatt ctcttgcacg caagggttac agagttgtag
    16981 tggtttcagc ggacggtttc tctgcagaac tcgattcggg cctgatggcg agaaacgatc
    17041 gaataatact cgcctacaag aaaaacggtc agtatctgtc gaagaacgat ggcccgctgc
    17101 ggcttgtcgg agaagatctg ccttcgaaga aacaccaagt gaagcaggta gttgaaatca
    17161 ggttagaggg catatagcgc acagtacttt gttgatctgc aatattggta accgttcgcc
    17221 agggcagtat tgtgtggagg gctgacatgg atgaatccag gaccaatgcg gaaagcctac
    17281 tatttcagta cgcgagatct cgtccttatt gcggctatta gcgccgctgg gggcgtcctc
    17341 agcacttacg ttggttatct gggaaacctc atgaacaagg ctttcggtgt gccctttggt
    17401 gccggtcaat ggatggcagg actccatgta ttctggttcg tgtttgccag ggcgattgtg
    17461 gggcgaacgg gagcggcatc ccttactggg atccttaaag ggatagtcga gatgtttgcc
    17521 ggcagcaccc acggatttcc aattgtaatt gtgtcagctg ttgagggaat tcttgttgat
    17581 gtcgtgttgt tgccttcggg aagtaaccct tcccttgggg caatgtgcct cgctggcggg
    17641 atttcgtctg cttccaacgt gctcatattc caggccctgt acttttcagg ggtatcaatg
    17701 ggctatgtgg ggctgataac cgcttttgcc ttcgcatcgg gggcagcgtt cgggggatat
    17761 ttcactgcag gcgtgcttga tgctgtgtca tccgcccaca tacttaaggt cgatgaagag
    17821 cgaaggagcg tccatcgacc gccagttgtc cgcatgggcg ttgccattgc gctagtgctg
    17881 gttttggccg gcggagcagt gtactactat gcatgcgtct tcgaaccttt ctggaatggg
    17941 cctacatgcc gtgtcgaggg cacggtggct caagcttaca attacagacc tattgatttt
    18001 gaagacgaca atgtcacggt cgtcgctgag ctcaagggtg cagtcaccca cgtacctccc
    18061 acggattaca caggagtgcc cttgactgcg attcttgaac gcgcaggcgt gggcgagtcg
    18121 gccacagaga ttcaggttgt tgcttctgat gggtattacg ccgtgttcga cctcgatgat
    18181 gttatgcgcc gtcaagatgt cattttggcg caggatgacg aaacgctcag gttgattgcc
    18241 acagggtacg agggggccta ttgggtcagg atggtatcca ggatcgtggt gtgcgaggat
    18301 gattgacatt tccggcctta gagtgcggta ttatggctcc tccgaactcg ctctaaaaga
    18361 tattgacctc atagttgaac ctggcgagag cgtcattgtg actggcccta gcggctgtgg
    18421 caagtctaca ttggcgctct gcttggcagg atttgttcct catgccattg gggctgaagt
    18481 agcaggcagc ataagggttt gcggcattaa tcagcgagaa gccagcgttt accggattgc
    18541 acagcatgtg ggccttgtgc agcaagaccc tgaagggcag ttctgcacca tgtgcgtgga
    18601 ggatgaggtt gcatttgggc ctgagaatct ccgttgttcg ccttatgagg tcgaaagccg
    18661 ccttgacagg gcgttggcaa cgacaggagt atctcatctt cgggggcgca agttgactga
    18721 gctttctgga ggagagaagc agaaagttgc cctcgctgca gtgctggcca tggaacctga
    18781 tctcgtgatt ttggatgaac ctacagccta tcttgatccg cgtgccaccc gtgaacttat
    18841 cgagcttgtg tcgaggatgc gggatgcgga tagtaggttg acactcttga tactggaaca
    18901 taaaccatgg cgttttagcc atgcactgga acgggtgatc catcttgaca ggggttctct
    18961 cacgtacgat gggcctatcg gcggtgccgc tttcgaagag gagatcctgg atcagctccc
    19021 acacagccac agatcgcttc acggctttgg cgagcctttg ctcagagtgc agaacctctg
    19081 gcaagcctat ggcgagagaa ctgtgcttcg cgatgtagat ttggaggttt gcagaggtga
    19141 gattgtggga ctgatgggcg acaatggaag cggcaagacg actttgctcc atacaattat
    19201 gggattctta cggccctcgt caggcagagt actgcttgaa ggggatgata tcaccaacaa
    19261 gccggtatca gcccgggcca gaagaatcgg aatgatattt cagaatccaa accaccagct
    19321 gtttacggac tgtgtgtgga gggaggtggt cataggtccc ttatgtcaag gtgacactga
    19381 agaagagtgc cgtcctcgcg cagatgcgct ccttgactca ttcggactct ctagttaccg
    19441 cgattcccat ccgctactac tgagctacgg ccagaagcgc cgactcaacc tcgcgtcaac
    19501 catggcatcc aacccaagtc tcctgttgct cgatgaaccc tttgccgggc aggatgtatc
    19561 gcgagcccgc gatctcatgt gcattctgga tgagcttgct tcagagggcg ttggagtctt
    19621 aatcataggc catgatcccg atatcatgac caggtgttgc gacaggatcc ttttcatgcg
    19681 tgatggaaag attcagatag acgctcctcc ttacgaggct gtagctgagc ttgaaaggag
    19741 gggcgaacat gattaccttc ccggcagatt ctgacggctc aactgctgtc agcaaactga
    19801 accccatcac taaactgatc gtattagtgt cggcttgtac agtagctttg cggcttaagg
    19861 agcctttgca tgccctgatt tgttttggcc ttattctcat tgggcatctt ctcactaagg
    19921 tgagaccggc catcttgtgg cagcggacga gaggcatatt catgtttggc atcatccttt
    19981 tcgctagcca gatcgctttg gtatgtgatg tgccttggca aactcggatt gccaacggag
    20041 ctttgatgtc tttgcgcttt ctcaatgtgg tcctggcaag ccatctcttt gtaagtgtca
    20101 ctagctcgga ggattttgcc tacgctctca tgcaggccgg cctcccatac agatacggat
    20161 tcgccctcct tgccgcaatg cgtttcgttc catacttcaa aacgcaggca aatacggtac
    20221 ttcaggcgca gatggcgcgg gggatggaag tggatcgccc cacgccgaaa gggatattcc
    20281 tcatggccaa gcacaccttc gttccagtca ttgtgactgc cctgagcaag gttgattccc
    20341 tcgccatatc tatggaaggc aggtgttttg gcctttactc tgaccgaact taccgccgga
    20401 gaatcccttt tcactggtgg gacggggttg ttatatgtgc aagcattgca ggcatagttg
    20461 cgtctttcta tcttatctga gcggagcgaa gatctctttg cgcattgaac ctccgtggca
    20521 tgaatgctaa cattgtggtg ctgaacgaac gagccagttc acgggctgcg gagggcatat
    20581 gagcgatgaa atatgaattg attctgtttg atgctgatgg aacgctattc gattacgaca
    20641 aggcggaacg gtatgcactt gagcaaactt gtcgccaaca tggactagga tacgatcaac
    20701 ctttgctgga acgatacagg gctatcaatt cgtcgctctg gaggcagctt gaggagggca
    20761 tgatcatttc ctcaaagatc agggtggaga ggttcaggag gcttctttct gaaatcggat
    20821 cagatgcaga gtgtaactgg gaggaattcg cccagcatta cctctcgcat ctcgccgggg
    20881 gtgcctggct catagacggg gcagaggaga tctgccgcta ccttgctccc tattacaagc
    20941 tggccatatt gaccaatgga atcaaggaag tccagatatc gaggtttcag gcttctccgc
    21001 tcacaccata catcaacaag ctgatcatct cggaggaggt ggggtacagc aaaccccgtc
    21061 ccgagatctt tgaatatgca atgaagaccc tgggacagga gtgtcgaagc acaacgatga
    21121 tagtcggcga ttcgctcact tcggacatgc gtgggggcaa ggactttggc atcgcaacat
    21181 gctggtacaa tccacatggc attgaaaatg gcacgggctt agaggttgac tacgagatac
    21241 gccggctgga tcaattgaag gacatcttgt gattggatgc ctgagttagc tcggctccat
    21301 gaaggccctt ctcttttgct atatcgatgg ctttgcggag ttctagcttc gcagggtctg
    21361 aatggcattt tgagcccaac tgggattggc cagaagggcc cttcccacgc ctacaaggtc
    21421 tgctttgcct tgacggacta acgagtcggc ggcttctgga gttgttatgc cacccgttac
    21481 catcaccgga acgtccactg cgccttttat tgcctcagag agcggcgcaa agtagccctc
    21541 gcctgtgtat cccggcaagc tggcgccacc gaggccacct gagacgtcga tagcgtcgac
    21601 tcccgctgct accagttttt gtgcggccca acatgtgtca tcgattgtga gcccgccagg
    21661 aacgccatcg tcagccccca gcctgtagaa tatggctgcg tcaggtccga ctgccgaaca
    21721 gaccgccgtc acgacttcga gaggaaaacg gagacgtccc tctcgactcc ccccatactc
    21781 atccgatcga atgttggtga tgggagaaag aaactggttt agtaggtagc catgtgcccc
    21841 atggagttcg accatgtcga atccggcggc aactgcgcgt ttggcgcctt gcacaaatgc
    21901 tttcacaaga tgcggtatga agctgcgctc gatttcacat ggaacctcgt gttcgggtga
    21961 aagcgggacg accgaaggac ccaaaggcct ttgccctatt gtgctgctgc gggtggcggc
    22021 gccggcatgg gtgatctgaa tgccaattgc ggcgccgcag gcgtgaacgc gccgagttag
    22081 ctcgctgagg gggcctacat ggtcatcaca gtctatcccg agctgccttt ctcggtattt
    22141 gcccgacttg agcacgtacg tatgctctac gatgatcagt cctaccccgc gatccgcgcg
    22201 ctcttcatag tagctgaacg tagcttcagt tgccatgcct tcaagagttg cagtgttgct
    22261 tgccattggc ggcatgacta tgcggttttt caagctcata ttctttacac aaagcggcga
    22321 cagaagatct gacaatgtta gtggagcctc ccgtattgat attgttgatg ctgccttcac
    22381 gcgacgaatt cgattgtctt aacgtacacg agctgggcaa gccttcgccc cgaaatccga
    22441 agctaagccg aggcttgctt cggatttgcg tcattgttag cccggtcatc cgtgcgtcag
    22501 tcgaaaagaa cctcaccagg aattactctg ctgccaatta gccatcggat tcgatccgaa
    22561 atctaacggt aggagagtag aacatgaatc ggcccaacca agctttgaat gttgtccaca
    22621 agccagcatc cagccgcaga aggcttttaa ggcgtacaga cgggctcagc tgagccgagg
    22681 tcatagcata gtctatgtat gcgttgtcag tcgcgttgct taggctgttc accgcggaca
    22741 ccgtagggaa gcggtatata ccccactgat ggccttttcc gggaagcagc ttgcgggtaa
    22801 agctccatga gatcgtatat ctccctaggc atgtctgtgc gtaccgaaag gcccatctgt
    22861 ttgagctctt cacatgtaat tggatagtca tgagtccagc gcccagtaga aagctgatcc
    22921 gtaatttctg cagccttttc gggcggcatc ttgtccgcca agatcttcat aatgaggcca
    22981 tggacttgct tcaatgcttt ctcggataca tccgcgagta ttagggtttg gtcatctaac
    23041 tcggcaaccg gctttctggc cacagcggaa agtatcgatg ctgcgggata tgagcctatt
    23101 tgagggtcaa ctgggccaag cacggcattg ggatccataa ctatttcgtc ggcagccagg
    23161 gcgataagtg tgccgcctga catggcgtag tgagggacga atactgtaac tttggccgga
    23221 tgattcttta tggcatgggc aatctgctcc gtggccagga cgagtcctcc cggagtatgg
    23281 aggacaatat cgatgggaac gttcgggtca gtcagcctta tcgcccgcag aacctgttcc
    23341 gagtcctcaa tatcgatatg gcggccgaag gtcatcccga ataggtttgt cgattcttgc
    23401 ctgtgtatca gcgtgatgac tcgggatcgt cttctcttct cgaaattccg tatgacacga
    23461 tatctatgag aatctcgtgt ctgtcgagaa agcaatggca cgaaactcca cacgatgata
    23521 agaagccaga agatgtccag aatagtgatg cgcatgtgct cccctcccga tcatttgtta
    23581 ttctatgttt ccgcatatcg attatagcct tattatcctt ccaattccat aaggctagta
    23641 gtaatctgct gatgatggtt gtgcgcatgg gtgttttccc agggctaggc ggaagagcgg
    23701 agggatgctc atgatggcac ttcgccagag atgacacatg gggttagcta gagtgaagat
    23761 ttggcaatgg ccgtggcgaa acagggaagg ccccgatctg ctgattttcg ggcgaaccaa
    23821 agagtgtact ggccacttgc atgttgcgta gccaaggccc tgcgcagtgc ggcaggacat
    23881 cgagcttgtc catgttcttc actcagttgt tgctccgtcg gcaagttgag ggcgcgatcg
    23941 gattgcgtcg cgttctccat gaaaatcggg accggcatgg gctatcgcta actccacatg
    24001 ccggcctgtg ctacgaggct gagagaatca taggggatgg cccccagacg ccatatcgct
    24061 attttgttaa tgcctagtct ggaaagggta tccaatttga cagccaaaga catgagatct
    24121 tcataccaaa cccgatgcaa cctgcctgaa gcgtcgtggt agacaaatcc cgaagtccca
    24181 tcatgctggt cgcgcttgat caaggcgcca gtttcccgtg atatccgctc cgctgcttct
    24241 actctcagat aagctgcgcc ctcgtcatcg gtccaatcga gtccgtagat gccaagcgcg
    24301 acagtgagtt tgccggtccc gatcttgtct atagcggcct tcacattctc ttccagccat
    24361 gacacgggag tcggtgggcc ttcgacggag tccgcccctt tctcgtagcc ttccattatc
    24421 attaagtcgg catattgagc catgcaggct aagtcaagcc catggtcgcg ccaatcgtgc
    24481 gggaatacta ttgcaatggt gtagtagggg ctgagtctag tgaacctgga acggatttcg
    24541 catgctagct tgggcagcat ggcccaatga tctgcatcga tttgctggaa gtcgatgcaa
    24601 accccgtcga ggcaacaggt ggtgacagct tccacaatcg atgctgcaag atgtgaacgg
    24661 ctatcgtctg ccagcagggt tgcggtaacc gcctccctag aataggtggc atccgactgg
    24721 ttggtgactc cggccaacac gcgtgtgcgc cttgcccgaa ggatttggag tgcgcgatcc
    24781 agggcggccg ccctcactgt cccatctggt tgtatgcgga tgaaagtgta tatgatttca
    24841 ttgaaaggtt ccggagacgc gagggcgtct gcgatccctt ccgcaaccgc gtaatcgggc
    24901 acaaacccga tcagcctgtg ctttcgcggg atgtgaagca cttgcccggg acgcacgtcg
    24961 tacggctctt ctatggagtt agcttgtgct atcgctcgat agtcatggcg gaatctctgc
    25021 gctatgagcc atagagtatc tccgctttgg actgtataat gcattcatgc cgcctcctgc
    25081 gagcggtgat aacctgaaca ctacaagcta tgcaaaaggt cataaaaaag ttcccgctca
    25141 tggagacggc atgcttagta tatttgcgcg ctatgagaat ttggttgttt tctttgtcgt
    25201 cccgtatcta tccgtgactg ctccagttct gagcagcgcc tcagatacaa gcactgcgag
    25261 ggcgcctcct gccagcgccg tgaagagctg cggggagccc atggctttcg cggctgcttc
    25321 aggcaatgac agaaggtgtt tggcgcttaa tgagatgaag agaaacttga tcacagaagc
    25381 gaccgcgata cctagaatgc ggatggacag actcttgcct ttgcctgttt cgtctatgag
    25441 gccgaagatg atgcacaaag atgcatttcc tagctggata acggggacca ggggccctgc
    25501 cgccattatc cctaccatcc atgcgacaag cggcgttgca aggcctataa cggctccgct
    25561 ccacatgcca acgaggatga ctgagaggaa tagaaacatg ttgactacgg gtccggtaaa
    25621 tgcttgaggc aacttgaaga ccacctgaaa cgcgagggtc atagccaaca gaattgctgt
    25681 tcgagttatc cagttgactg gctttcctga catcagcacc acgctcctat gcctttagta
    25741 tccgtaaatg agcagcatgc ctgcgagtat tccgatcact attccgaagg ttccagagtg
    25801 tccatcagcg aattcctgag cgtcaggtat gagctcgtca gcggtgatga acaccatggc
    25861 gcctccagca aaagccagag agacggcgag catgccattg gagatgtttc cgatagcagc
    25921 gcctacaaat gcccctaacg ccattggcag acctgcgagg gcgccatata gtgtgctttg
    25981 ctttctgcca acgcctcctg cacacatggg tgcagccatg gccatgcctt ccgggatgtt
    26041 gtgaatggca atggtgagag ccactgaaag gccaagcgag ttgccatgag cgtaacccgc
    26101 tccaatagcc aacccctccg gcagattgtg catggcaatt ccgatccccg tgagaagccc
    26161 tgctttgaga aaccgattct gcttacagtc ttctgagacg aagtggaaat ggggtatcag
    26221 aagatccatg attcctgtgg ccccgacgcc gagcaaaaaa ccgcctactg tctgcgcaac
    26281 cccgcctatt tctatggctt cggggataag gtcgaggaag actattgaca gcattactcc
    26341 ggcggctgtc cccatgagcg tcgagagagt gcgccgcttg ggagtccccg atgctgctgt
    26401 cagaaaagcg ccgaaccctg tgccgaggac gcccgctgca agccctagaa gtgttgcatt
    26461 acagatgtgc tgcaatttag cacctcctgc tcttagcata ttctacgtcc atgcacctcc
    26521 catgtcaaaa ccgaatgtac aggagattag ggcacatgat ggacgagatc gctgtatggt
    26581 ataattgcgc ggagagggtg gtcggatgat tctcgcaatt gatgtgggca ataccaatac
    26641 tgtgctaggt gtattcgatg gcgaagaact ccttgcagac tacagagtat ccacagacag
    26701 gcaaaggact gctgatgaat ggggcatgct tttgctggaa ttgcttgacc atgcgaaaat
    26761 cgatggggct gcgatttccg gagtgattat ctcgtcagtc gttcctccga taatcacgga
    26821 acttgcgaag atgtcgcgca ggtactttcg gtgcgatcct gaagttgtag ggccggggat
    26881 gaagacggga atttccataa gatacgaaaa cccgaaagaa gtgggggccg acaggatcgt
    26941 aaatgccgtt gccgggtacg agctgtacgg cggtcctcta attattgtag atttcggcac
    27001 cgccaccact ttttgtgcta ttggtcaaac aggcgattac cttggcggcg ctatcgcccc
    27061 cgggattatg atatcagctg aagctttgta tatgagggcg gcccgtcttc ctagggtgga
    27121 gttgacgaag ccgcagtctg ttataggcaa gaacacagtt tcaagcatgc aggccggaat
    27181 cctatacggc ttcgtgggcc aggttgatga gatcgtccga agaataaagc aggagcttgg
    27241 gggcgatccg aaagtgattg ccactggagg gcttgcggaa gtgatatctc ttgagtcaga
    27301 tcatatcgat gaggtcaatc aaaacttgac gctaataggg ctgcgcattt tatgggagag
    27361 gaatcattct taggctcagc ggggtgatat gttgaatacc agtttcagcc ttgagggtgc
    27421 aaacaacagc tcgttgcatt ccggatcagg cgacaatatg gaaagggcgg tgttggtggg
    27481 cctggagacc gccgctcctt ctgcgtggaa catagaagac tccataggag aactggcaca
    27541 tttgactgaa actgctgggg cagccgtggt cgccacagtc atccagaaga agggcaaacc
    27601 tgatcccgct tattacgtgg gagagggcaa agctcgggag atcgagatgg ttcggtcggc
    27661 gacagaggcc aacctcgtca tcttcgacga cgaactgact cctgcgcaac agcgaaatct
    27721 ggaggagatc atagaagggc gaataatcga caggactgag ctgatcctcg atattttcgc
    27781 tcagcgtgct gcgacatctg aaggtaaact ccaggttgag ttggcgcaac tcaattacat
    27841 gcttccaagg ttggctggcc gagggacaga gatgtcgcgg cttgggggcg gtatcggtac
    27901 ccgggggcct ggagagacca gacttgaggc ggaccgcagg acgatcagaa accgtatatc
    27961 cagcatcacc cgcgagcttg agacggtcag gcagcgccgc acggtgcagc ggcaaaagag
    28021 gcgtgatgcc gatctcccgc ttgttgcgtt ggtgggctac accaatgcag gcaagtcaag
    28081 tttgctcaac acccttaccg gggctgaagt ccttgtagag gacaagctct ttgccaccct
    28141 cgatccgacg actcgtcgat acacgctccc tgacgggcag gcgctcttgc tcacggatac
    28201 cgttggtttt atacagaaac tgcctcatca tttgataatg gcgttccggg ccacgatgga
    28261 agaggtcgtg gatgctgata tgctgttgca cgtggtcgac gcttctcacc cgcaggcagt
    28321 ggagcattgc gaagccgtct ttgatgtatt gcgcgagatc gatgccgcgg accatccggt
    28381 catcactgtg ctcaacaaat cagatttgcc cgaatcgggc ccgatgatag agaggctgca
    28441 tcatgcctac ccgaaaagtg ttgccgtttc cgcagtaaca ggcgacgggc tcgatgatct
    28501 tgctgagatg atatcggagg agttatccag ctggagggtg aggatcgagg agctcgtgcc
    28561 ctacggtgat cccgtgctag gactgatcag agcaaggggc agggtaatct ctgaagtgta
    28621 tcgcgaggac ggcgttgcca tcgtggcaga agtagatcgc atggtcgcag gacaggtaag
    28681 aaaggcgaga tccaatggct ctaagcgcca gtgactcctc ttttcggcgg gagcccgatt
    28741 ccgttcttgc cgatggtttt gccatcggcg gcgtgcgctt gatgaatccg cttgtgcttg
    28801 cgccaatggc tggcgtgagc gatgccccct tccggcggat atgcaaagag atgggctgtg
    28861 cccttgtcta tacggagatg atcagcggca tggccctagt atatcgaaat cggcgcaccc
    28921 tggacatgct gcatgtctcg gaagacgagc accccattgc catacagctc ttcgggtcgg
    28981 atccgcgtgt aatggccagc gcagctcgga ttgtgatgga tggcggagca gacgtgatcg
    29041 acatcaacat gggatgtcct gtgcctaaga tcgtgaacaa cggtgagggt tcggcgttga
    29101 tgcgggatcc tgagagagcg tgggctattg ttgcagctgt gcggcaggcg gttgacgttc
    29161 ctgttacagt caagatccgc aagggatggg atgactccag cgtgaacgct gtcgagtttg
    29221 ccaagggttg cgtggatgct ggggcggacg gggtcgccgt tcatggccgg acgcgtgaac
    29281 agggctacag tggcgatgct gactggaaca taatcgctca agtcgctcat gcggtggatg
    29341 tgccagtgat cggcaatggg gacgtaatca gcgccgagaa ttctttcgaa atgctggaga
    29401 gcacggggtg cgccggggtt atgatcggcc gtggggcact gggtaaccct tggatcttcc
    29461 gagattgtct gcattatctg gctacaggca cgcatcttcc tcaacctcag ccccgcgata
    29521 ggatttctat ggcattacgc catctcgatg acgctgtcga tctcaagggg gaaaaggtag
    29581 ccgtgcgcga gatgcgaaaa cacatggctt ggtatattaa agggcttcgg ggtgcagccc
    29641 gtatccgcga ggcaataaat gcttcggagg acgctgaagc attgcgtagt attatgagtg
    29701 cttacttgag acaacttgag gacggcgttt gaagcagttt cgtgggcaat tgactgttgc
    29761 ggagggaatg tcgcctttga ccgcctcatg cctgggcggt gttttgttct gcgtctgtgc
    29821 aaggaaacac ccgaaaaaca cttgtttgca ggtgcgtcct ctggtaaaat cggtgtgtgc
    29881 acatagatgt gaacattcct acgtctgtct tctgcggggg gtgggcttga cgaatctcat
    29941 acgcattggc gacaaggttg ttgacaggga caggattgac agggccctcg ataagctgtt
    30001 tgatatgcga cagaacggag cgtcccaggc tgaagtggct gcagccttag gaacagatag
    30061 atcattcgtg tcgcgccttg agaatctcgg cgagattcga aagggcgaac gagttgcggt
    30121 catcggcttt ccaatcggga acaaagaaga gatagaacaa ctggcgaaga aagccggggt
    30181 tgactacgtt tggctcatga atgacgagga acgttgggct tacgtagaag acaggtccgg
    30241 aatagagctg ctcaatgatg tgattcgcga gatatcagat tttaaccagt atgatcaggt
    30301 gatttttctt ggctcagatt tccgggtccg tctcatcgag gcgattctag gtgagaaggt
    30361 aacatcagta gtgatcggaa agtccccaat caaggaggat gtcaacatag atgtggcgga
    30421 aatgatctcc atcattaagg ctgtgcgggg aggcgctgaa aaggcatgaa gcacgttgtc
    30481 agtgttagca taggctcatc cgatcgtaac cattcagcca ctgttaactt gctcggagag
    30541 gatatccttg tggagcgaat cgggaccgac ggagatatga ataaagcggt cgagctcatc
    30601 agcgaactcg acggaaaagt tgatgctttc ggaatgggcg gcatcgatct ttacatggcg
    30661 ggggtcggca agagatacgt ctttcgggat gcaaaaagga tttcccaggc agcaaagaag
    30721 acgccgcttg tggacggcac aatcctcaag agcagtttgg agtataacgc agtgaagtat
    30781 gctgccgagg ttgaaaagct acccatggcc ggcaagaatg tattcgtggt gtgtgttatc
    30841 gatcggatag gcatgggtcg ggcctttgaa gagctcgggt gcaacatggt gtacggagac
    30901 ttgatgttca gcaccggcat gcatattccg ctctattcac ttaattccat atataagttg
    30961 gcgcctatat tcatgccaat aatgactcaa ctcccgttta gttggctcta tccaacgggc
    31021 aaggaacaga atgccagtcg caagcggcca aaggacaaga aacccaagtt tcagaaatac
    31081 tacaatgacg ctcatgtcat tgcaggcgac ttccattaca tatacaagta tttgcctgag
    31141 gatatcagcg gaaagatagt cgttacgaat acggtgactt cgaaggacgt tgatgagcta
    31201 gggcggcgag gagctagcgc tctggtgacc acgacgccag agatcaatgg gcgctcattc
    31261 ggggtcaacg ttatcgaggc aatactcgtt gcgttgatcg gcaaacctgt cgaggatatt
    31321 agtgacgagg attttacggc gatggtacaa tgtctcgaga taaagcctcg tattgtgagg
    31381 tattgatgag ggcgagatgg cagttttgcc attagatgtg cgaacctata catgtgtggc
    31441 agaccctggc ttcttgacaa cctggggtct gcttgccata atattggcgt gacttttggc
    31501 gggagcgcca tcttttttta ctgcagcgat ttatggatct ttcgggtttg aatctgtccg
    31561 acggggcgcg aatacaatgg ttatcatcct agatggtggc taggaattga cgtttgaggg
    31621 gagcttggta tagatgtctg acaaggaagt gatcctcact cccgaaggcc tccataacct
    31681 cgaaaaagag ctcgagtatc tcaggacgac aaagcgcagg gatatagcgg cccggattaa
    31741 gcaggccata gagttcggag atatcagcga caactctgag tacgaagatg ccaaaaacga
    31801 gcaggctttt gtggaggggc gcattgctca gctggaaaag atgctgagga atgccaagat
    31861 aatcgaagga cctgatggag atggcgacgc ggtttctgtg ggttccaccg tagtcttgaa
    31921 ggatttgaac catggtgatg tagaacagta tacgatagtc ggttcagccg aggcaaatcc
    31981 tagcagaaat cgtatttcca acgagtcgcc tgttggtcgt gcagtgctag gaaagactgt
    32041 aggcagtatt gtggaggtaa atgttccggt gggaacaatc agatacgaag tagttcagat
    32101 tgcaaggtga gtgaagataa tggacgaact tggaggacaa tttgaaggcg ctgaactagg
    32161 gaatgaactt gcccatagga aagcgaaggt tgaggagtgg cgctcacgcg ggattgaccc
    32221 gtatggctcc aagtttgtca ggacgcattg cgctgctgag attgttgagg acttttcaga
    32281 gctggagggc gaagaggtat cgatcgctgg gcggattatg gccttgaggg gccatggaaa
    32341 ggcttcattc cttgatattg tcgatgcatc cggtcgcata cagctttatg ccaagcacga
    32401 tgctttaggg gatgagctgt acgagttgct tggaatgatg gacatcggcg acatcattgg
    32461 agttacaggg actgtgttcc gtaccagacg cggggagatc agtgtagcca tatcttcgtt
    32521 ccgcatactt tcgaaggcgc tacggccgtt gcctgaaaaa tggcatgggc ttcgcgatgt
    32581 ggatacccgt tatcgccagc gttatcttga cctcatggtc aatccggaag tgcagaggac
    32641 ttttgttttg cggactaaga taatatcggc gatccgcgaa tacctcgatt ccagaggctt
    32701 cctagaggtt gagacaccgt gcatgcatgc gatcgccggc ggtgcagcag ctcgaccgtt
    32761 cattacgcat cataacgcgc ttgatatgga attatactta cgtattgcta cggagctgca
    32821 tctgaagcgt ttgatagtag gcgggtttga aaaggtgtac gagcttggcc ggatattccg
    32881 gaatgagggc atctccacaa agcataatcc ggaattcacg tcgatcgaag tatatgaagc
    32941 ttatgcagat tactacgata tgatggatct tacagagggt cttgtggcgt acgtggccga
    33001 gacagtgttg ggcactacgg aagtggagta tcaaggagat aagatcgatt ttgcgccccc
    33061 ttggacacgg attaccatgg ctgaggcagt cctaaaggag acaggcgtcg atttcctcaa
    33121 catcgaacag acggaggcgg tagcggcctc caggaagtta gggctgaaag tgtctgatga
    33181 tgctacttgc ggcttctgca tgagtgaagc gtatgaggag ttcgtagaga gccgtctggt
    33241 gcaacccact atagtcatgg attatccgat agaagtgtcg ccgcttgcca aaagacgggc
    33301 tgacaacccc aatctcacct acagatttga agtggtatgt gccggacgcg aattggctaa
    33361 tgccttcaca gaactgaacg atccagagga tcagctgcag cggttcagcg ctcaagttgc
    33421 ccagcgcgag aagggcgatg atgaagcgca catgatggat gaggatttcg tgacggcact
    33481 cgagtatggg atgcctccta cgggggggat ggggcttggg gttgacaggc ttgtaatgct
    33541 cctcaccgat tcgccatcca tacgagatgt gatactcttc ccgttgatgc gtccgaaaga
    33601 ataaggtgga ggcgaagcgt cttgcaggtg cttgtatatt ctgattatga tgagatgagc
    33661 gcggcggccg cgagaatgat agcgtctaag atcggcgagc tccccaagcc ggtgctgggg
    33721 cttgcaactg ggtccacgcc aatcggcatg tatgctgagc tggtgcggct tcacaatgag
    33781 gaaggtttgg acttttccaa cgtcaccacg ttcaaccttg acgaatattg cggactcgac
    33841 gctgaggatc ccagaagcta tcattggttc atgaaagagc atcttttctc ccatgtgaac
    33901 ttggatcctg aaaaggcgca tgtgcccgcg ggaaacagtg cagatcctga cggcgaatgc
    33961 gcaaggtatg aaaggctgat caaagaggcc ggaggcattg acttgcaggt tttggggatc
    34021 ggaatgaatg ggcatatcgg gttcaacgaa cctggctcgg atccgaactc gaggacccgc
    34081 gtggtgcgtc tggctgagca gacaatcgag gttaacagag ccaagggcgg actgaatgcc
    34141 ttgccaggtt atgcgatatc catgggtatc ggaacaataa tggaagcgcg ccagatattg
    34201 cttctggcaa atggcgcagg caaggcggag attattcggc gtgcgcttac cgggccggtt
    34261 acgcccgatg tgccggcttc cctgctccag acgcatccta acgttgtgtg tatgcttgac
    34321 aagccggcag cgtcattgct agatctttct tgacgcaggt atgtgtgtat ggtaggatta
    34381 taagcgcagc aagtttggat gggcccgtag ctcagctggg agagcgctag aatcgcactc
    34441 tagaggtcgc gcgttcgatc cgcgtcgggt ccaccagttt aatatgtggg tttccactta
    34501 tccggtcgtg gcattcacga ccgggctttt tttatgctcg catctgactg ttattcggtg
    34561 gcgtgcatcc tgacgaaacc atgtattttc aaggttggac aagacttggt gctgctatag
    34621 ccgttggttt tgccggtgtc gtcctttgac ccggcaagcg tcgagcgtac gaagtagcca
    34681 cggaagtatg ggttgtgtac tgatgtgaca tcgtataaaa tggaacaggt ataatccttt
    34741 ggaaggaggg ctcacggttg gcgtacgaga tcaacgataa ctgcattgct tgcggtgcat
    34801 gcaaagagga atgtcctacg gaggccatca gcgagggcga tatttacgca atagacccag
    34861 agaagtgcat tgactgcggt acatgcaacg aggtgtgccc tgtaggagcg ccgaatccag
    34921 cttgaggaaa aggctatatt gaattggcaa tccggttgtg gacttctcga tgtaccatgt
    34981 acgttggggg gtccatgatt catattatga caatccacgg gcagaaacag atgaaaaccc
    35041 gaccaggctg cttcctcagt ggtattctct gaagtctgcg gatgtgggtt tgccgtcatc
    35101 agccgcgccc tcaaaccccg cgggggcggc gcgtgttgtg cgcgcattag cagtgtagta
    35161 gaataaatga tggatttcgg tggttggagg ggtgtagtat ggcaatcggg gagcttgtga
    35221 attctgtcga tagatctaag ttggagtttg ttgagaagct ccggggcgga gtgatcatgg
    35281 atgtcaccac gccagagcag gctaagattg ctgaagacgc cggggccgtt gcagtcatgg
    35341 ctcttgagag ggtgcctgcg gatattcgcg ctcagggcgg cgtagcgaga atggccgatc
    35401 cggccctgat agagcagatc atggattcag tttccatccc tgtaatggcg aaggtgcgga
    35461 tagggcattt tgtggaggct cagatactag aagcgatagg cgtagattgc atagatgaga
    35521 gcgaagtcct gactccggct gatgagagat atcatgttga caagcacgct ttcacgacac
    35581 ctttcgtgtg cggttgccgc aatctcggcg aggcgctccg caggatcggt gaaggcgctt
    35641 caatgatgcg gacaaaaggc gaggcaggaa caggagacat agtagaggcg gtccgccaca
    35701 taagggcggt taatgaggat attcggcggc tcaagaatat gccttgcgaa gagataatgg
    35761 ccatggccaa agagattgga gccccatacg agctgctttg ccaagtggcc cgggatggca
    35821 agctcccagt cgtgaatttc gccgccggtg gcgtggcaac tcctgcagat gccgctctaa
    35881 tgatgcaatt gggagccgac ggcgtttttg tgggttcagg catcttcaag tcggatgatc
    35941 cggctgccag ggccagggca atcgttggcg cagttgcaaa ctatagggat gccaaagcgc
    36001 ttgccgaggt ttcgaaaggc cttggagaag ccatgaaggg gatagatgtc tcgtccatgg
    36061 ccgatgagga aaagctgcag ggcagaggct ggtagcgctg tctatagcat gtagattcgt
    36121 ctggctttgg acctggcccg ggctttcgta agccgaaggc cagattttgc gaaggcgggg
    36181 ataaatgcat gtacgataat ataaggcgcg aggatcccga agtagcagat gcgataagta
    36241 acgaaattcg aaggcagaat gagaagatag agctgatcgc atccgagaac tttgtcagtg
    36301 aagcggttct cgaggcgttg ggttcgccgc ttaccaacaa gtacgcagag ggttacccgg
    36361 gtcgaagata ttacggcggg tgtgagcacg tagatgttgt cgagtctctt gctattgagc
    36421 gcgcgaagcg gctctttgga gcggaacatg cgaacgttca gccacactcc ggtgcgcagg
    36481 caaatatggc cgtttacttt gcggccctag aacccggcga tactgtcctt gggatgaact
    36541 tgacccatgg cggtcattta acccatggaa tgcacctgaa tatttccgga aagtatttcc
    36601 acttcattcc ttatggcgtc agggaagaca cgcaaattat cgactatgac gccatggaaa
    36661 accttgccat agagcatcgc cccaagatga ttgtggcagg cgccagcgca tacccgagga
    36721 tcatcgactt tgagcgcata agcgagattg cccacagtgt agatgcactt ctaatggttg
    36781 atatggcaca tattgccgga ttggttgcgg ctggtcttca tcccaatccg gttgaatacg
    36841 cggattttgt gaccaccacg acccacaaga ctcttcgtgg gcctcgcggg ggaatgatat
    36901 tgtgcaagtc ggaatgggct aggaagatcg attctgcagt tttcccaggt acgcagggcg
    36961 gtccgctcat gcacgtcata gcggcgaaag ccgtggcttt cgcggaagct ttgcagcccg
    37021 agtttgctca gtatcaacgt ggtataatta tgaatgcgca ggctctcgcg gacgcgctga
    37081 tggaacgtgg atggcgcctg gtttcaggcg gaactgacaa ccatctaatc ctgatggacc
    37141 tccgcgggac gggaattaca ggaagagtcg ccgaggaagc cctcgatgag gtgggcatta
    37201 cggttaacaa gaatacagtt ccgttcgacc ctgaaaaacc catggtgacc agcggtgttc
    37261 gaatcggaac ccctgctgtt acgactcggg gcatgaagga gcctgagatg cgggagatcg
    37321 cctcactgat cgataagacg ctgagaaatc ttggctcaga gggagttatg gacgaagtca
    37381 ggcaaggtgt gaatgacatc atatctaggt tcccactgta ttcccgaatc agagccgagt
    37441 tgagctgata tacaggtttt ttgttgttga atttgctcgc gaatcacttg tgccgcgggt
    37501 acccgcggca tgattcatac gaactataca agctgtgtac gagttacccc ggccagctat
    37561 gtatggatga acgatgaaca caggataggg ggaggaaggt tatggctaag ctcctagaga
    37621 ttcgctggca tggacgtgct ggtcaggggg cgaagacggc tgcacttgta ctggctgagg
    37681 cagctatctc cacaggcaaa tacgcccagg cgttccctga atacggccca gagagaatgg
    37741 gcgcgcccat gaaagcttat aacagaatca gcgatgaacc aatcacagtc caccaccatg
    37801 tggaagagcc agatatcgtc atagttctgg atccgacttt gctcacagtt ggcaatgtca
    37861 aggccggagt ccctgacgat ggaatctacc tcgtcaacac accgctgtcg cccagcgaga
    37921 tgcgcacaag actggggctt gaaggcagca aggcgaaggt atacaccctc gacgcgaccc
    37981 aggtgtctat ggacaccatt ggccgcaata tcccgaacac tccaatgatc ggtgccctta
    38041 ttgcggcaac ggggcttttg gagctcgatg agctgcttga ggatactcgg agaaaactct
    38101 ccaagaagtt gcgccccgag gtggttgaca agaatatgga ggctattaag cgggcctatc
    38161 gggaggtggc ttcttaatgt cagttcctaa ggatgccggc tggaaggacg tgccgaaggc
    38221 cggaaggatt ctagaaccgg gatgtgccga taagtatgaa acaggcggat ggagaactta
    38281 ccgccctgtt catatagaag aaaactgcat caattgcttg ttctgttgga tttactgccc
    38341 tgatcaggcc atccttgtag aggacgggaa gtttaagggc atagactacg cttactgcaa
    38401 aggctgcggc atatgcgcaa aggaatgccc aggtaagcgt ggcaacaagg cactcaagat
    38461 ggtcttggat gaagaggcct agacgaagta aggaaggagg agcaattcat gagcataagg
    38521 gttggaaaaa ccggaaatga ggcagtcgca gaggcgatgc gccagatcaa cccggacgta
    38581 gtcgcagcgt acccgattac gccgcagacc gagatagtac agatcttctc cagcttcgtc
    38641 gctaatggca aggtggatac cgagttcgtg acggtagaga gtgagcactc cgcgatgagc
    38701 gctactgttg gagcctctgc agccggcgcg cgagcaatga ctgctacatc ggcaaatggt
    38761 cttgcgttca tgtgggaggt tgtatatatc gcggccgcta tgaggctgcc gatagtaatg
    38821 ccggtcgtga atagggcgct ttcggggccg ctgaatatcc actgcgacca tagcgacacg
    38881 atgggggcaa gggattctgg atggatccag attttctccg aaaatgcgca ggaagcgtat
    38941 gataacgcca ttcaggcgct taggatcgcg gagaattcca atgtgctgct gccggtcatg
    39001 gtgacgatgg atggtttcat tatcagccat gccatggaag taatggagat cctcgaagat
    39061 gagaaggttc gggagttcat tggagaatac gttcccgaga accctctcct cgacgtggac
    39121 aacccagtga ctattggacc catagatctg caggattgga atttcgaaca taagagacag
    39181 caggctgagg ctatgcgcaa cgcgaaaccc gtgattttag aggttgctaa ggattacgcg
    39241 aagctgactg gtcgcgagta tggcctgttt gaagaataca ggatggaaga tgccgaggta
    39301 gctgtggtgg ttcttggatc tactgcgggt actgccaagg ctgtcgtgga tgagctgagg
    39361 gccgacgggg ttcgtgccgg cttgctcaag ataagggtat tccggccatt cccggcagaa
    39421 gagatagctg aagcgttgaa agacgtgaag gccgtggctg tgatggacag atcggattcg
    39481 ttcaatgccg tgggcggccc ggtctttggc gatgtgcgct ctgctctgta tgacgtcgag
    39541 aacggtccga ggatcgttga ctatatctac gggatcggcg gccgcgatgt gactcctgat
    39601 cacatccgaa cggtctatgc cgacctagca aagatcgccg acaccgggca ggttgagaat
    39661 ctcctcacct accttggggt cagagagtag ggggtgcgca catggcgact ttgaaggaac
    39721 tgtctaagaa ggaggtcttg atctcatcag gccaccgtgc ctgtgcgggt tgcggatttc
    39781 cggtcattat aaaacaggcg ctcctggcgg cggagaatcc ggtggttgtg tcgtgcgcaa
    39841 caggatgcat ggaagtatgc accactatct acccgtatac ggcgtggaag gttccattca
    39901 tccacaacgc gtttgagaat tctgcttcca cccttagcgg cgtagaggca gcatacaagt
    39961 ccctctcgcg tcagggcaag atcgacaaga agatcgactt catagccttt ggcggcgacg
    40021 gaggaacgta cgacatcggt ttccaggcgc tttccggagc tatggaacgt gggcacaaca
    40081 tgctatacat ctgctacgac aatcaggcat acatgaatac agggattcag cggtcgagcg
    40141 cgactccgag gggtgctaac accactacta gccctgcggg aaccgagtcg tacgggaaga
    40201 agcagtatcg caaggatctc agtgcaatta tggttgctca tgagattccc tacgtcgcgc
    40261 aggcttcccc aagcaattgg aatgacttgg tcagcaaggt aaagaaggcc ctggccgtcg
    40321 agggccctgc cttcatgaac atcatccagc catgtgttct cggatggaag tatccttccg
    40381 atcagagcat ggaactggcc aggttggctg tggatacctg tttctggccg ttgtatgagg
    40441 tggaagacgg agaatacagg ctcacctaca agccaaaaga aaagaagcct gtggaagatt
    40501 ggcttaagag gcaggataga ttcagacacc tcttcaagcc ggaaaacgcc gagatcgtca
    40561 aggagatcca ggaggatgtg gacgaacgtt gggccaagct cctggccaag tgcggcgagg
    40621 aggcgtaaca gcctgacagt ccatacagct taataggctg ttgttttcac taacgagcgt
    40681 gaagccagct ggtcgtctca atgtcggggc gcctggctgg ctttatgaat gtgctgagag
    40741 tctgtgccgt gccgcaggca ttggcgcagt taaggggagc accgccttag acgtgcaaat
    40801 atcgttgcaa tgcagcgttt cgtaggctcg cgattgaggc atgccctggg cgaaccttgt
    40861 gatagcgagt ttacatgtta agcaacagct caaagcggct atcttgttct cagcccaaaa
    40921 cccttgtttc gtcaggaatt gagcacacct cattttacga agcatgctgg agtattgcgt
    40981 catggtgggt ggggatgatt gccagtgcac tgcgattgcg acttatcagg agagcagtat
    41041 gagggggcaa ttctctgttt aggtgcggaa aggatctccg cacccgaggt tgaaggtatt
    41101 cgaatttgat acgctaagtg aaggcgaagg tgcggcatgt aatcagggta tgtgagcttt
    41161 tcgatatggt caacggagac aagatcctaa tcggcatgct tgggcttgcg tccggcattg
    41221 ttatgcccta gggcctggat tccgtacgaa gaccctcttc cgtaattttt ccgtcaagat
    41281 gatgtccccc ttgttcatg
//
LOCUS       DAOSYL010000014        38962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_840_length_38962_cov_8.610559, whole genome shotgun sequence.
ACCESSION   DAOSYL010000014 DAOSYL010000000
VERSION     DAOSYL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 38962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38962
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_38962_cov_8.610559"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLU15_04010"
     CDS             complement(<1..492)
                     /locus_tag="PLU15_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOO30117.1"
                     /translation="MKEDLVAALDGGGTKTVLVIADSCGQVLGIGKGGPVNALFVPDS
                     TAIESVQNAAKGAMERAGLSDEADAPVHVRAVYASVPGASREIIEAGLSGLVAYDVLQ
                     VEGDDYATFRGAFPEGYGVVVLAGTGSFAMGRSPDGRTLTAGGWGPLLGDEGSGYAIG
                     LAGL"
     gene            complement(521..1150)
                     /locus_tag="PLU15_04015"
     CDS             complement(521..1150)
                     /locus_tag="PLU15_04015"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO30118.1"
                     /translation="MAEGRILHYFGPGHSHMLGEELFYRAGGLAPVNAILEPALMVNY
                     GAGKSSKMERLPGLAKIVLDESSVKSGDVIIVASTSGVNQVPVEMACEAKAAGVKVVA
                     LTAVEYSRATPLRNDRNVRLCEVGDVVIDTLVTYGDAAIEVDGVAQKIAPVSTVIGAV
                     IVNAITIGVVENLVARGIDPPIFKSANIPGGMEHNAKLLERYRPMIKSL"
     gene            complement(1261..1992)
                     /locus_tag="PLU15_04020"
     CDS             complement(1261..1992)
                     /locus_tag="PLU15_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO30119.1"
                     /translation="MELNHDLPTPLYQQVYDIWKRKIETGELRPGDRIPTERELCEMH
                     GVSQITVRQAIQMLVQEGLVIRRPRTGTRVAQRKFSQQLVKLTSFSEDMRSRGLVPGG
                     RVVLASEEEADVATARALRLDEGSLVVMIERVRLAEGEPMAIEIFRMPAALCPGLLDE
                     YIEDKSLYELLRTRYHIDPIWAKQSFEASLCKSREAELLGIRRGAPVLRVERIAYDSS
                     ETPIEYTASVYRGDRYKLHVYMNTR"
     assembly_gap    2307..2406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2643..3182)
                     /locus_tag="PLU15_04025"
     CDS             complement(2643..3182)
                     /locus_tag="PLU15_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO30120.1"
                     /translation="MAFSGSPRARGNTRTLIDEVLRGVASAGADCEVVDVASADIRPC
                     RACQSCSTNGICVQDDDMRSIYPKIAEADVLVLGTPVYFWGPSAQMKAFIDRWYALVN
                     GDDGNRLKGKKAIIVTAYGDDDPTTPEHVIGMFRTAFHYTGIDLVGVLGVTASAPGDA
                     ARNAQAMREAFELGKGLGN"
     gene            complement(3240..4226)
                     /locus_tag="PLU15_04030"
     CDS             complement(3240..4226)
                     /locus_tag="PLU15_04030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOO30121.1"
                     /translation="MKPRVYVTRRLPQEGMDMLEGGCEVGFNAHDRGMTREELMACVK
                     DADGLVTLLSDSIDAEVMDAGPKLKVIANYAVGFNNIDIAAATERGIAVTNTPGVLTE
                     TTADLAWALLMATARRIVESDRFMRSGRFQGWAPMLYLGHDIYDKTLGVVGFGRIGQA
                     VARRALGFNMKVLYSGRQRAPEEIERACNASFCQLETLLQESDFVSLHVPLTESTHHL
                     IGAAQLDMMKESAILINTARGPVVDEKALVWALKTGKIAGAGLDVYEHEPDFEPELAE
                     MDNVVMVPHIGSASVATRTKMATMVARDAMDVLGGRMPAHLVNPEVWDRRRI"
     gene            complement(4253..5203)
                     /locus_tag="PLU15_04035"
     CDS             complement(4253..5203)
                     /locus_tag="PLU15_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO30122.1"
                     /translation="MRTVVTGATGLLGEEVADWLAGQEGHEVIGLCGRRDLDVVDTRA
                     VVSFMCRAQPDLIVHCAAERDMDAAERDPKAAYMLNTFGTWNLALAARLCDCKMVFIS
                     SEAVYSGEKDAPYTESDLAAPLSVYGWSKLAAEEEVKSLLERYFILRVPLLFGAKGHS
                     DNNRILNFVRALRSGKCVTAPIDQWTSPTYARDVAMAIGCMTRSWAGGRRVSCCFSNG
                     SSERTRAQSEYGVYVVANQGKASRYEFACKLAELASCDGSLVRPGSREGKPARRTKNT
                     TLDCARLKRDFGFALRPWEDALSECVQSMGLFSQNMLRQL"
     gene            complement(5380..6300)
                     /locus_tag="PLU15_04040"
     CDS             complement(5380..6300)
                     /locus_tag="PLU15_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOO30123.1"
                     /translation="MLLCTHRKGLVLSIIALLAFGYGLFGASCCAGAGVPVASDGGLV
                     GSKRITVMTYNIHYGVGFDFVYDLDRIADLIEEEGADIIGLCEVEQCTKKFNYQDQAG
                     HIADKLGFYYAYGPIFARKDGGYFSNALISRYPIVSHQTHQLPIFNDNQKRAVLEAQV
                     DVDGTLLNVFVTHLEVKDHESRMAQAQALLEIASRTECPRIIMGDFNTIPTSSVIALM
                     LHEYNDTYQVYRVLTDSAEIASEGLFERDYLKGGYTIDPFDPNRRIDFIFSSFDIRVV
                     GEPKAARVHHSMASDHLPYIATLELPAASL"
     gene            complement(6808..8079)
                     /locus_tag="PLU15_04045"
     CDS             complement(6808..8079)
                     /locus_tag="PLU15_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30124.1"
                     /translation="MKSHGKLVRMLRATLLTICVLLLFAASTGLAARQETVTMWAFPL
                     CSDDIEMYSKLVNEFEKENPNIKVHVEVLPWQGRMEKLMAALAANQAPDVMYLNVFLV
                     PKLAATGNLAPLDKYLPSDVRADYFSGAIKSCQYDGRLYAMPVLQSVVTYIYNVDLFK
                     KAGLDPKAPPETWAELEEAARKLTCDTDGDGKIDQWGIRFDLNRPSPITSVVPFVWQA
                     GGDLISADGTEAIYDSPEAVRAVEFIKGLIDKGYMQKSNIQGGGLPFTSGKIGIDLQR
                     ENMDVIKLRAEGLDFEIAAGPILKDAKKIGYGTVGSYGILGKSKNKDAAAKWVLFLTR
                     PDNMAYIMKNTGFVAPRKSVSPAKYVTDPVIKQFVEQAPNCRPETPHLYIQEVLSTMQ
                     PAIENALLGLKPVEAALKEGTAEANRIMRRK"
     gene            complement(8115..8960)
                     /locus_tag="PLU15_04050"
     CDS             complement(8115..8960)
                     /locus_tag="PLU15_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30125.1"
                     /translation="MNRLRKMPMKNFWTYFILVLLGSTMIVPFIAMVSQSLKPGSQVL
                     EYPFRWIPENPSMENYRMLFKQNPIMRWTLNSFIVAGGSTILQLITCSLAAYAFSRTS
                     FPGKDILFWMMMSMLIIPSQTTLVPLFLLFSKLNLTNTYTALLLPTASSAFGIFLLKQ
                     FMEPIPKDYDEAASLDGCTRFQFYSKILLPMCKPALAVLATFNFVTQWNDFLYPLIMT
                     QTRDMFTLQIGLSALQASGSRGEMGGMGVVMAGAVWGFIPTFILFLLLQKYLVEGVSS
                     AGIKG"
     gene            complement(8974..9843)
                     /locus_tag="PLU15_04055"
     CDS             complement(8974..9843)
                     /locus_tag="PLU15_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30126.1"
                     /translation="MNTLSELRKSRESYYFLAPSVIIFLVFGLSVLVANVVLSFYSWS
                     ALTEPRFIGLDNYRRLFHDAVFRKALVNTLYYCALYVVPSLIISVLLAFVLNKKLKGM
                     NLFRSIFYLPVITSYVIVVILWQWIYDADIGLLNSILNKIGIASVPWLLSPKLALPSL
                     VLMSIWKNVGYTIVIFLAGFQQIDVALHEAARVEGATEWQVFRHITLPLLRPTTLFST
                     VMLIIWSFQMFVQPYMMTHGGPARSTTTLVYYLYETGFQYFDLGYASTMSVVMLIMVL
                     PIIILQSKLLRAR"
     gene            complement(9876..10958)
                     /locus_tag="PLU15_04060"
     CDS             complement(9876..10958)
                     /locus_tag="PLU15_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO30127.1"
                     /translation="MRYRAGIVGCGRIGWKLEDDPNRPKPCTHAGAYASIPNTQIVAA
                     SSRTDKSLKSFGERFGVDALYTDFNEMLEKENLDLLSVTTNPELHRDIVVKAAECGVR
                     GILCEKPISLSLDDADDMIEACRKSGTVLMVMHNRRWNSYYVKAKELLDLGAIGELVS
                     IFGNCQGCKPSPDWLSDREGPLLHDATHLYDIMRFFAGDIDSVVGSAEKRKHLHYGVE
                     DTCFSHMRFKNGVSAVALVNERTSYMRFDIELQGSDGKILLGPDAEMWQVVPSKRAAG
                     FRELEKVEFPKPEREANVYMDAIGELIHCVETGEPSRSTGEDGRAALEAIMAIYESQR
                     RGNSPVQLPLGVRESLLLALRRENRL"
     gene            complement(10993..11985)
                     /locus_tag="PLU15_04065"
     CDS             complement(10993..11985)
                     /locus_tag="PLU15_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO30128.1"
                     /translation="MSHDRPRIGIIGLGLSNPYTFARILKAEGADVAYVWDYHRDAAE
                     AFARENGCEAAPTWQELLKAPLDGVLVESINADHCKLAKAFVEQGVPAYVEKPLSFDY
                     EEAKDLLDTAVSNDCPILSCSPLRFSPVFQEIARSVREGRLGLPTLAHARVIHTMHHF
                     ATDPRKRWHDSLSLGGGMVVDIGVHAVELGYMMLGPGAASVYCETSRVKYKDIESEDT
                     AAITIKFADGAIAVCDVVCATNELEYSVMVNGSEDSIDSRELCAKLCNEGYGTPDGIG
                     NALSAYGGFVETMRQFLKCIDRRCFPVPPLEMGEIVKILSAARASAKQHSPVLI"
     gene            complement(12013..12915)
                     /locus_tag="PLU15_04070"
     CDS             complement(12013..12915)
                     /locus_tag="PLU15_04070"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOO30129.1"
                     /translation="MTADFELKGIIPPLVTPLDDDGALDEQAFRRSIRRMVRAGASGI
                     FILGSSGEFAALTDACKARVIETAVDEVGGKMPVLVGVGDTSTARVFENMQAAALPGV
                     SAIVVCTPYYFPTQQEALVKFYEDIAEKSQVPVVLYNVPQTTKTWIGIETVRRLTDNP
                     NIIGIKDSSGDFDYHQRLISLASSASRFSVLQGDELSIGASVLMGGHGAVPGIANLDP
                     KACVDLYDAAASGDAATAVKMQSGVSSLCEVYKHGGSPVGALKACLKMMGEGSGKLTH
                     PLDDQKPLDDLEILSRILSEHGLI"
     gene            complement(12912..13634)
                     /locus_tag="PLU15_04075"
     CDS             complement(12912..13634)
                     /locus_tag="PLU15_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOO30130.1"
                     /translation="MRLQGEGVVQKIEHKSVSQQVEEAIKRYIVQKGLRTGDRLPTER
                     ELAEMLGVSRSSVREAIVGLQALGIVESKPKVGITVSEPNVASLIHAVAFNSGLNTEK
                     IFQLNEARLVLETAALKLAVEKADQSDYEAMAETIYRMEAKVEAGESCAAEDLAFHQA
                     IVEATHNPILSQSSHTLQEFFHIISAEGVGAGVSPARILSEHKEIYNAFRQKNVGKCE
                     ELMTRHLQRYYFAQRQEGDTEK"
     gene            13841..16453
                     /locus_tag="PLU15_04080"
     CDS             13841..16453
                     /locus_tag="PLU15_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO30131.1"
                     /translation="MSVPGVPKPEVAVRPVLDSKQRAFVDCQHGCVVLHAPVGTGKTL
                     ALAERAAEAIRHGVDPNRILCVTFTNRAAEELRQRITVSCGEHARGVVVRTFHSLCAW
                     MLRSEAKQMSLPSGFVIFDDDDSMEIIRQCAKRTGVHLVCSGYDDEARDAQDAITTAK
                     INAPDAVLSTNSVQDAVFDSLPRAWRRLALAYQDELAAHHALDFADLVYFARAMLSVE
                     PDIANRWAERFSMIQVDEMQDTHVSEYQVLRVLAKKYGNIALAGDFDQTIYEWRGSQP
                     EKIITLLQKDFPQARRFSSVTNYRTTRTLVDAAASVAANYSDFDRVRPCATAEEGNPI
                     VVHFARDSQAEAQWIAEETRKLIEPGESANNIDPLRPGRIAVLTRTNWRGTVISEAFA
                     EAGIPHLTVEQFEFFRRQEVKDAIAYLRFLRNPFDGRSFQRMLLRPPRNIGRQTIDSV
                     IEAERTGLRLIDMVNPSTIELGDPFARLMRELDSGSVVVFDTETTGLNPASDDIVELA
                     AIRLEHGQPVATFHRYLRNRVSVGDSRHIHGLSDQFLAAHGADPLETLSQFMAFADGA
                     LLAGHNVSFDVRMFRANARRLGLNPPSHQCADTLDIARRFLDVDDYSLANLTRYFNIP
                     CKPTHRAMDDVNATTALLIALAPYVNKGSIGRMQAVRETGESFVPLAREIEEMLMLAE
                     RVRPDALLDEVLERSGLNAYYIKEPRRMENLRELVRIFRDKDDVRLDPISSLESILHF
                     VALARNVDRVDPEDERVRILTVHQSKGLEFDVVFVAGLSEHEFPGYRSVKDGRQDEEL
                     RLFYVALTRARSRLFLTGHAVNNGKHREPSPYFGTISDGWVEQDSHAIRRTYGSGNRR
                     NPQSLQSKGHRRPF"
     gene            17223..17927
                     /locus_tag="PLU15_04085"
     CDS             17223..17927
                     /locus_tag="PLU15_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOO30132.1"
                     /translation="MRRRTFVAMIVLIGLFASPYALSAEEQQHLMPATIIRVLDGDTV
                     EVILENGARQRVRLIGVDCPELTDKDPSVRDYAFAAAAFTRELLEGRRVWLETDMGTL
                     DKYGRLLAYVWTAPPADFAEEKIRDCMFNAILLLEGYAYTYMLMPNTKYAAVFVGFQH
                     EATMEGRGIWGTMRRWIHIIVYVTNTGKKYHGDGCRYLASSRIPMTLAEAMWRRYERC
                     QTCKPPYWMSAGISLE"
     gene            complement(18033..18902)
                     /locus_tag="PLU15_04090"
     CDS             complement(18033..18902)
                     /locus_tag="PLU15_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO30133.1"
                     /translation="MSFGIVTDSSANLTDEMIDKYGIDIISLSYRIGEEECQSYVKGE
                     KTDLKQFYEKLRQGEVAETSLINMRDCENAFESVLQEGKDLLYIGFSSALSGTYNAAS
                     IVADNMREIYPQRKIYAIDSLAASMGQGLLVYHAVEQRLAGRSIDEVKDWLLENRLRL
                     CHWFTVDDLMYLKRGGRVSVSAAVIGTVLSVKPVMHVDDEGKLVPVRNVRGRKKSLVA
                     LVDEMEKTCIDPTEQMVFISHGDSLEDAECVEKLVRERLNVRDVRINYVDPVIGAHSG
                     PGTIALFFLGTKR"
     gene            complement(19147..20061)
                     /locus_tag="PLU15_04095"
     CDS             complement(19147..20061)
                     /locus_tag="PLU15_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOO30134.1"
                     /translation="MGHRRRSVLFAVFLAVLMIAFATTAHGNSAVGEAGPGNPTEIGV
                     DEKEMVSDPKAEATAKRPLVIAHRGASGVAPENTMAAFERAVEMGADMIELDVHATKD
                     GQIVVIHDAVLDRTTTGSVRGSVVWRTLEEIKTLDAGSWKGAEFAGEKVPTLHEVLST
                     FKGRTKVLIEIKAKGIEDKVVEIIRETKTEDDVIIQSFDVEAVRRCRNLLPQVPAGVL
                     FSAPLLFGKSSFGEKVISTALDVNADFVAVDQGGVTPELVGMARENGLGVFVWTVNKT
                     KDMTKMIQLGVDGIITNHPDVLIGLLAE"
     gene            complement(20387..21787)
                     /locus_tag="PLU15_04100"
     CDS             complement(20387..21787)
                     /locus_tag="PLU15_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO30135.1"
                     /translation="MLKRINALFGAQDMTEGKPMSVLLRFSIPLLIGNFAQQMYSTVD
                     SIIVGRYVGDKALSSIGTTLPVINLLLVLFMAVSTGAGIMVAQYYGAKDKESLSRSIG
                     NAMTLVFISSLVIMAVGIPLAGQLLTLTKTPVETFDMAYSYLTITLSGILGVGYYNIV
                     SGILRGLGNSVFPLLTLLLASILNIFMDIWFVAGLGMGVAGAAWATIISQAVSAVLCI
                     AKLRRMKDVVNLNASNLVPDKKLTGQLLRLGLPAGITQAVFSMSMVFVQALANSMGYQ
                     VVTCNTAVMRIDGFAMMPNFTFGMAIATFVGQNIGADKMDRVAQGTKDILKVSLTTSF
                     VLVACLLLFGENLIRMFTTTEEIINLGVRQIRILAAGYVAMAISQVFGGIMRGAGDTM
                     PSMWISMFTVVVVRVPLAYIWASLTRSPDHPAGSPDALFFSLLISWIAGAVCNYLWYR
                     RGTWREKSLVAHEQAA"
     gene            complement(21968..22891)
                     /locus_tag="PLU15_04105"
     CDS             complement(21968..22891)
                     /locus_tag="PLU15_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOO30136.1"
                     /translation="MKHVVSVSIGSSSRNHSARITLLGEEILVERIGTDGDVAKAKQL
                     IGELKDKVDAFGLGGIDLYLEAKGRKYYFREAKNIAAMAGDTPVVDGTGLKSRVEYNA
                     VMSVATEWGLPIAGKDVFLVCVFDRIGMGLAFEELGCRMTYGDLMFATGIPIPLYKIS
                     SVHALAPIVMPWLTQLPFSWVYPTGKSQRANRDKAKKRKLKFQQYYNRAHVLAGDFHY
                     IYRHLPERIDGKIIVTNTVTEDDVEDIGRRGAHALVTTSPEIQGRSFGANLMEAMLIA
                     LIDKPIDEITDEDYDDMMKRLDLRPRVVMYD"
     gene            23583..24047
                     /locus_tag="PLU15_04110"
     CDS             23583..24047
                     /locus_tag="PLU15_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30137.1"
                     /translation="MWNELAVEAITILLWAATALVVLFLSGLSKYLQEQARRVENDAA
                     RGLIQSALSEAEKVGIDAVQATNQVFVDAIKASSADGKLSKEDAATAMKIAVEYFKTH
                     LSSRSYQVLSEPDGKLDDKWISDFLEARLAQTKAESSRCAYPLPAPLPDCPE"
     gene            complement(24489..25247)
                     /locus_tag="PLU15_04115"
     CDS             complement(24489..25247)
                     /locus_tag="PLU15_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOO30138.1"
                     /translation="MEKKQVAAELDRILRLDTKPVGIKLYKNQEDLPRKPFNFKLNLC
                     QLVAMARYQGKTNSGTPDKMICAMGAACVGLIDTPEAISSGKAAVGAYVKDQTAGKTF
                     MDNTFKIGDTGKRYDGILVGALESMEEDPDVVVVYVNPAQAMRLIHACAYETGEKITA
                     DTVAEAALCSCIGYAVANDKPVVGFPCAGDRIFGGTQTHEVVFVAPYPLVREKLVENL
                     ERTAEGGFSVYPVPPNMFWTPTMPPTYTIGPEDL"
     gene            complement(25631..26875)
                     /locus_tag="PLU15_04120"
     CDS             complement(25631..26875)
                     /locus_tag="PLU15_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOO30139.1"
                     /translation="MTLREQALELHRANRGKIEVVSKTPLRTKEDLSLAYTPGVAEPC
                     KEIHAEPDKVYDYTNKGNMVAVVTNGTAVLGLGDIGPAAGMPVMEGKAVLFKSFAGVD
                     AFPICLDTKDPDEIVNVVKCLEPTFGGINLEDISAPTCFEVEDKLKAALDIPVFHDDQ
                     HGTAIITCAAMINALKIVSKNASDVRVIVNGAGAAGIAVTKLLMALGVRDVVLCDSKG
                     AIFEGRTYGMNPYKEEIAAITNPNKLSGSLADIIVGADVFIGLSVAGCVTREMVASMA
                     KDAIVCAMANPVPEIMPDEAKAGGAKVIATGRSDFPNQINNVLAFPGVFRGALDARAS
                     AINEEMKMAAVYAISDIVTEDELSPEYIIPGPFDPRVAPAVAANVAKAAQASGVARVK
                     VLPEEVSKRTVELVEAVKASWK"
     gene            complement(27099..27743)
                     /locus_tag="PLU15_04125"
     CDS             complement(27099..27743)
                     /locus_tag="PLU15_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOO30140.1"
                     /translation="MKILIASIDEKDLIGAKDYGVHGIITNPTIVAEVRKPWKEAVSD
                     AAKIIEGPFHLQITEDKRDGIIAQAEEFASVLGDRLIVKMCISQESLAAMQVLQDRGL
                     KVNLTGIVSIPQSFFAIQAGADFISIYMGRADDVGGDGVGVVRSAANMAKNGGYKTEI
                     VAASIRGVSHYIEATEAGAHWAACPYKVLPKLIQHPVTDKSIVGFAQDWDRVIK"
     gene            complement(27848..28828)
                     /locus_tag="PLU15_04130"
     CDS             complement(27848..28828)
                     /locus_tag="PLU15_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOO30141.1"
                     /translation="MEIEQNPSSEHELLVRVARMYYEHDLNQQEIAERVGLSRSRISR
                     LLTRAREVGIVQVTIVDEFQEEHGLSRELESMYGLKRVVVCPISESSPKPLRWHLGQA
                     AARLLKEIIRDGDVIGVTGGTTMLEMARALKPLPIKRENVKIVQLEGALSGKGEALIH
                     GNEIVAKVAAAFDAPPYFLSAPTIVESRELRDALARDPNLRRILEKGRQANIAIFSVG
                     RPSKSCILAQAGYLSAEDIDVLLSKGAVGDICSRFFTADGSVCDESLNDRTIGLELDE
                     LTQKESCILVAGGPKKIPGIAGALRGGFANMLVTDEITARTLVDSADGAL"
     gene            complement(29091..29402)
                     /locus_tag="PLU15_04135"
     CDS             complement(29091..29402)
                     /locus_tag="PLU15_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30142.1"
                     /translation="MAEEKPLGFFAAWKATDTWYSASEDEKREFDRKVEDIFSRAREK
                     GVKMHGVYDCCWSTEWKYFTFWEVPNLELLEEVMEELSEIGDINMYNAQRHFVGRKVR
                     D"
     gene            complement(29444..29941)
                     /locus_tag="PLU15_04140"
     CDS             complement(29444..29941)
                     /locus_tag="PLU15_04140"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOO30143.1"
                     /translation="MHKVKVEELTKEAFAPYGQVVEMPEGAKKVSAVEDLWPGLVKWE
                     LDHETLEIAHGVCKKRPLIVEGLERHNTTYELLVPIKSDLIIPVASEDDRANKDAQPL
                     ARNVRGFLVKVGQAVFYDKGVWHLSPYPVEDEGPFFVVFKHKTAPDNIIMKELDEKVE
                     LEYEN"
     gene            complement(30046..31002)
                     /locus_tag="PLU15_04145"
                     /pseudo
     CDS             complement(30046..31002)
                     /locus_tag="PLU15_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(31099..32061)
                     /locus_tag="PLU15_04150"
     CDS             complement(31099..32061)
                     /locus_tag="PLU15_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30144.1"
                     /translation="MEKTQGFDYKTFIEKYGALAILALCMIVLTIISPYFLTALNLKT
                     IAIQTAVIAIIAIGQTFVIITGGIDLSVGANLALSNILTASMMTSGVPLWICLVTALA
                     FSAAFGYVNGLAITKMRVPPFIVTLGTMSIARGIALVMTNGTPISRLPEALSWFGRGE
                     IVGLPVPVLIFIVAAIIFNWVLNHTLFGRYTYAVGSNMEATRLSGIDTGRTVRMVYLV
                     SGLLAGLAGIVLMGRLGLAHPTAGADYNMNSVAAAVIGGTSLTGGVGNIFGTMIGALI
                     MGVLDNGFVLLGLSSFYQEIAIGFVVIMAVYLDLLQQTKLRERR"
     gene            complement(32134..33621)
                     /locus_tag="PLU15_04155"
     CDS             complement(32134..33621)
                     /locus_tag="PLU15_04155"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO30145.1"
                     /translation="MEYMLEMNHIDKRFPGVQALSDVTLKVKPGEVHVLLGENGAGKS
                     TLVKILAGVYRKDAGEIILGGQKVDYSSTNDASKLGISMIYQELNMIPDLTVAENIFI
                     NREHLKGWPKHIDWEKMNSEAAKALKKLNANISPTQKARRLTTAQKQMVEIARAISAD
                     SRIIIMDEPTASLTEKEIGELFRQIRLLSEENVSIIYISHRLNEVKEIGDRVTVLRDG
                     QYIGTRDIKEADTDDLIQMMVGREITQFFPKCVAKKGKEALRVENLSRKNVLHDISFS
                     VHEGEVLGFSGLIGSGRTELMRAIYGADGDVSGRVYVDGKEAKVSSPKLAVQNGIAFL
                     TEDRKNLGAILNFPIYQNITLASMDQFSRNSVIDHQAERSRSEELFSMFNIKAPSIDT
                     VTRSLSGGNQQKVLLARWFCRDAKIIILDEPTRGIDVGSKVEIYEFINELAGQGKAII
                     MVSSDLPEVLAMSDRIAVMREGRLTGILDREEATQEKIMKLATLG"
     gene            complement(33723..34556)
                     /locus_tag="PLU15_04160"
     CDS             complement(33723..34556)
                     /locus_tag="PLU15_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOO30146.1"
                     /translation="MSAVPPKANALSEIFERPKPLIGTIHCLPFPGSPRYKGEDLAVA
                     INHAVEEALAYEEGGIDGIILENSGDYPFLAPGDVGFETVATMTAVACAVRDAIEIPF
                     GVILNWNAAIPAMAVAKASGAAFIRVNEWVNAYVSNSGIIEGDSGNILRYRKNIGAEN
                     VKILADVNVKHGAHALVADRSISDQAWDAEYYDADVIIVTGTKTGSAAPLEKIEAVRA
                     GTSLPVITGSGLCVENAEDIMPLVDGAIVGTSLKENGVWWGRVQVDRVKELVDKVSKF
                     R"
     gene            complement(34586..35503)
                     /locus_tag="PLU15_04165"
     CDS             complement(34586..35503)
                     /locus_tag="PLU15_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOO30147.1"
                     /translation="MLDIIGVGDASVDLIIKVDHLPGHDEKVRGELLGKYPGGIIANF
                     CCAASRFGAKTGIVTTVGDDDFGRIAIRGLAEFGVDQGGLVVKEGEPTYFGVIQLDST
                     GEKALVVVETPLLVPSIEDVDVDYLNNARYVHLTSLDLELAKFVASSVNNDVRVSIDI
                     EPTADSGGLDFWEDVLRKLHIVSLNEAGLKALVRCEDICKGAKTLLDYGPQIVVITSG
                     ADGVDVFSGDDHFHVPAFKVPVVDTTGAGDCFNAVFLSGLVKGWDLVKAVNYANAAAA
                     LSIQHVGARTGLPTEAVTRSFFDSKTCNR"
     gene            complement(36477..37877)
                     /locus_tag="PLU15_04170"
     CDS             complement(36477..37877)
                     /locus_tag="PLU15_04170"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOO30148.1"
                     /translation="MKAWEYQLEMRRRAKPVDFTENPRDWSASFEAFEYRKGMLALLW
                     ESEVPGSRAPEALLHAAVQAWHNRGYDVSEAERIAERGLKVCAGGDHGKLERITAELM
                     HALENAPKDPTHPYWSYNQPETWEEIMACMPAMKESLEDHAEDVDDSDVERRILGGWT
                     GQIVGGSYGTAMEGYTGDAIREAYGDRLNYYVAEPETYNDDITYELAFLAAADEAGPG
                     LSSRDIANKWLELIPSGWSAEYFALENLRRGIYPPESGRFRNYYSEWIGAQMRTMVCG
                     LVAPGDPLRAARYAYLDSVVSHEKNGVYAGIHSAVLTSLAFVVDDTRVLLKHARDYVP
                     DGTEFASVLDDAMESVQANKDHIESYKRFEEKLKTYNWIHAYPNMVAVVHSLWYCRND
                     FDKALRILADCGADVDCNAGEVCCAMGAMNPDCIDPRWTEPFNNQLETYLPGFESMEI
                     TALARWTYEIYRKLQA"
     gene            38250..38810
                     /locus_tag="PLU15_04175"
     CDS             38250..38810
                     /locus_tag="PLU15_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO30149.1"
                     /translation="MIQTSAAALGLASALYLDLDAIRLALVQTSSNEIELLTTGVLYA
                     AEGDDETASLALTRARSMVPDEPELLAQICIGFAILDRLDKALKTAKMAVAADDGNAA
                     AHYALGMSLFGMEDYPDALSNFERAVSLRPDYYLAHAYNGKFLARMEKWPESRPIPFV
                     KTSLGQMWTAQSLCSDSGPQTSPLPV"
BASE COUNT         9144 a        11270 c         9030 g         9418 t
ORIGIN      
        1 aagcccggca agtccgattg cataaccact tccctcgtct cctaggagag ggccccagcc
       61 accggcggtc aaggtgcgcc cgtccggaga gcgtcccatc gcaaaagatc cagtgcccgc
      121 aaggacaacg accccgtatc cctccgggaa agctccgcgg aaggtggcat agtcatcgcc
      181 ttcgacctgc agaacgtcgt aggccaccaa acccgacagg ccagcttcga ttatctccct
      241 actcgccccc ggcacactgg cataaacagc ccgcacgtgc acaggcgcat cggcctcgtc
      301 tgacagccct gcccgctcca tcgcaccctt cgccgcattc tgcacagact cgattgcagt
      361 actatcagga acaaagaggg cattcacggg gccgcccttc cctattccca gcacctgacc
      421 gcagctatcc gcaataacaa ggacagtttt ggtgccgccg ccatcgagcg ctgccactag
      481 atcttctttc atctgatgtt cctcccaaga tgaatcgtac ctacaacgac tttatcatcg
      541 gcctgtatct ttccaagagc ttcgcattgt gttccatgcc ccccggtatg ttagcgctct
      601 tgaagatggg cggatcaatg cctctggcaa ccaggttttc cactacacca atggtgatgg
      661 cattgactat tacagcgcct attaccgtgg atacgggagc aatcttctgg gccacgccat
      721 ctacttctat agctgcatcg ccatatgtaa caagcgtgtc aattaccacg tccccgacct
      781 cgcacaagcg cacgtttctg tcgttcctca gcggagtggc gcgggagtat tccacagcag
      841 taagcgctac tacctttaca ccagcggcct ttgcctcgca agccatctcg acaggcacct
      901 gattgactcc ggaggtggac gccacgatta tcacatcgcc ggacttgact gacgattcgt
      961 cgaggacgat cttggccagc ccgggaagac gctccatttt cgagctcttc cctgcaccgt
     1021 aattgaccat cagcgccggc tcgagtatgg catttacggg agctaaccct cctgctctgt
     1081 aaaagagctc ctcgcctagc atgtgagaat gccctgggcc gaaatagtgc aatatgcgtc
     1141 cttctgccaa agaatcagca gccaatctct acgttgccgc attgggattg atggactctg
     1201 gacagcaaag cctctacaag ctcgaaatac tcatccacca accttcgcct ccgcacctcg
     1261 tcaacgagtg ttcatgtaga catgcagctt gtatctgtct cccctgtata ccgatgcagt
     1321 atattcaatg ggagtttccg aagaatcgta ggcaatgcgt tcaacgcgaa ggacaggggc
     1381 gccacgcctg atccccaaca gttcggcctc tcttgacttg cacagggaag cctcgaaact
     1441 ctgcttggcc catatgggat ctatgtgata tctcgtccta agcagttcat acaaggattt
     1501 atcctctatg tactcatcca aaagtccggg gcaaagggct gcaggcatcc taaatatttc
     1561 aatggccatt ggctcacctt cggcaagacg aacacgctct atcatcacca caagcgaacc
     1621 ctcatcaagc ctgagcgctc gtgccgtcgc aacgtcagct tcttcctcgg aagctagaac
     1681 cacccggcca cccgggacta gcccccggga tctcatgtcc tcagagaaac tggtcaattt
     1741 gaccagttgc tggctgaact tgcgttgggc aacacgagtg cccgtgcgag gccgccttat
     1801 cactaggcct tcctgcacca gcatttgaat ggcctgtctc acagtgatct ggctaacacc
     1861 gtgcatctcg catagttcac gttcagtagg gatcctatcg cccggcctta actctccagt
     1921 ttcgattttg cgtttccata tgtcatacac ctgctgataa aggggcgtag gaagatcgtg
     1981 attgagttcc acgcataagc accacctaca gcggatctgg tccggggaaa ctcatgtagc
     2041 ggtacgtgat atattcttta tacttattat agatccttcc gtggcaaaaa gtttttctca
     2101 tccgcctcaa catccactca acaaggcgcg gttcgccgtg tgcggccgtc tatgagtcct
     2161 gtcccgcttt cgggtagata gtaaacctca catcgacgcc gcaatcccgc aaaacactca
     2221 ctattgcctc ggaaaacccc acggggaacc acgttgtctt ttgcgccatg gaacacccat
     2281 gttggcacat tggcgaggct ttgggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnncttt ctcccacatc atgcagaaac acgaacaagg acaatcctcg tgttagttgt
     2461 aatccttcgg caagtacaga aaataatccg ggcagacgtt cttcgcaatc tccttctcca
     2521 gcctcatgtc tttttgtccc atgaacaaca tcacccttct acgtccaagc atgtctgcgc
     2581 aacgcggttg tgcgatcccc acatcttccg gagtatacgc ttatacgatc tacgactcta
     2641 ttctagtttc ccaacccctt cccaagctcg aacgcctctc gcatagcctg tgcgttccta
     2701 gcggcatcgc cgggagcgga tgctgtgacg ccgagaaccc ctacaagatc tatccccgtg
     2761 tagtggaaag ccgtgcggaa catgccgata acatgttccg gggtcgtcgg atcatcatca
     2821 ccataggcgg tcacgatgat ggctttcttt cccttaagcc tgttgccgtc atcgccgttt
     2881 accaaggcat accatcgatc tatgaaggcc ttcatctgtg cagacgggcc ccaaaagtag
     2941 accggggtcc ccagaaccaa cacatcggct tcagcaatct tcgggtatat gctccgcata
     3001 tcgtcatcct gaacgcatat cccgttggtg ctgcatgatt ggcacgccct gcacggccta
     3061 atatcggcgg aggcaacgtc cacgacttca caatccgcgc ccgcgctcgc gacgccgcgc
     3121 agcacttcgt cgatcaaagt tcgcgtgttt ccacgagctc tcggactgcc cgaaaaagcc
     3181 acaactttca aacaaagcac ccccatcaac ttgccttaac acgaaacaac agtttcccgc
     3241 tagatccgcc gcctgtccca cacctccgga ttgaccaagt gtgccggcat ccggcctcca
     3301 agcacgtcca ttgcatccct tgcgaccatc gtcgccatct ttgttctggt tgcaacagat
     3361 gcgcttccaa tgtggggaac catgaccaca ttatccatct cagccagctc cggttcgaag
     3421 tcaggttcgt gctcgtaaac gtcaagaccc gctccggcaa tctttcctgt cttcaaagcc
     3481 caaaccagcg ctttttcgtc aactaccggg ccgcgggcgg tgttgatcag tatcgccgat
     3541 tccttcatca tatcaagctg agcggctcca ataaggtgat gtgtagactc ggtcaacggt
     3601 acgtgaaggc ttacaaaatc cgactcctgc agaagggttt ccagctggca gaaagaagca
     3661 ttgcaggctc tctcgatctc ctcgggggcc ctttgcctgc cggaatagag gaccttcatg
     3721 ttgaatccca acgctctgcg cgccaccgcc tggcctatcc tgccgaatcc caccactcca
     3781 agggtcttat cgtaaatgtc gtggcccaaa tagagcatcg gggcccaacc ctgaaacctg
     3841 cccgatcgca tgaacctgtc cgactccaca atccgccgcg ccgtggccat cagcaatgcc
     3901 catgcaagat cggcggtggt ctcagtaagc accccgggtg tgttggtcac cgcgattccc
     3961 cgttctgtgg cggccgcaat atctatattg ttgaacccca ccgcgtagtt ggctatgacc
     4021 ttcagtttcg gcccggcatc catcacctca gcatctatgc tgtcgctgag aagagtcacc
     4081 agcccatcag catccttgac gcacgccatc agctcttcgc gagtcatccc cctgtcatgc
     4141 gcgttgaagc cgacctcgca accgccttcg agcatatcca tgccctcctg agggagccgc
     4201 ctcgtcacat aaactcttgg tttcatatcc atctcactcc tctgcaattg ccctatagtt
     4261 gccgcaacat gttctggctg aaaagcccca tcgattggac acactcacta agcgcatcct
     4321 cccatgggcg aagagcaaaa ccgaaatctc tcttaaggcg cgcgcagtcc aaagttgtat
     4381 tcttcgttcg cctcgcaggc tttccctcgc ggcttccggg acgcacgaga gatccatcac
     4441 aactagccag ttccgcaagc ttgcacgcaa actcgtaacg cgacgctttc ccctggtttg
     4501 ccaccacgta aaccccatac tcactttggg cccgtgttct ttcggaagac ccgttcgaaa
     4561 agcagcaaga aacgcggcgc ccaccggccc aagagcgcgt catgcacccg atggccatag
     4621 caacatcccg ggcatacgtg ggcgatgtcc attggtcaat cggagctgtc acacatttgc
     4681 cgctacgcaa agcccttaca aagttgagta tccggttatt atcactgtgc cctttcgctc
     4741 cgaacagcag cggaacccga aggatgaaat acctctcaag caacgacttg acctcttcct
     4801 cggcagctag cttgctccat ccgtacacac tcaagggagc agcaaggtca gattctgtat
     4861 atggagcatc cttttcgcca ctatagaccg cttcgctgct gatgaagacc attttacaat
     4921 cacacagcct tgcggcgagc gccagattcc aagttccaaa ggtattaagc atgtaggcag
     4981 cttttggatc gcgctcagct gcatccatgt cgcgctctgc cgcacagtgg acgatcaaat
     5041 caggttgggc cctgcacata aaggaaacaa ctgcccgggt atcaactacg tcaagatcac
     5101 gtctcccgca aagtccaatc acttcatgtc cctcttggcc tgccaaccaa tcagcgacct
     5161 cttcgcccaa caggcctgtt gctccagtca ctactgttct cacaagctac cgcccccgcg
     5221 attgcattag tatgattcaa ataacacccc aactatgttc tccaccagct ttccaatgga
     5281 ttccttctcc agcatattgc cacagggcca ctccgcgccg gcgcacatat gcaaatcgca
     5341 tgtttcctcg aggctcgcct gcgcttgcgc ctttttctat caaagcgacg ccgccggaag
     5401 ctccagtgtg gcgatgtatg ggagatggtc agatgccata gaatggtgca ctcgcgcagc
     5461 cttaggctct cccactactc gtatgtcgaa ggacgagaat atgaagtcga tgcgccggtt
     5521 gggatcaaac gggtcaatcg tgtatccgcc tttgagataa tccctttcaa aaagcccctc
     5581 agatgcaatt tccgcgctgt cggtgagcac cctgtagact tggtatgtat cgttgtattc
     5641 gtgcaacatc aacgcgatca ctgaggatgt ggggatggtg ttgaaatccc ccatgatgat
     5701 gcgagggcac tctgtgcgcg aggcgatctc gaggagcgcc tgagcctgcg ccatgcggga
     5761 ctcgtgatcc ttgacctcca ggtgagtgac gaacacattg agcaacgtac cgtccacatc
     5821 tacttgagcc tcgagaacag cgcgcttttg gttgtcattg aagataggca gttgatgcgt
     5881 ctggtgggag acgatgggat accgactgat cagcgcattg gagaaatagc cgccatcctt
     5941 gcgagcaaaa atagggccat aagcatagta gaagccaagt ttatcggcga tatgtccggc
     6001 ctgatcttgg taattgaatt tttttgtgca ttgctccacc tcacataggc cgataatgtc
     6061 ggcgccttcc tcttcgatca gatccgctat tcgatccagg tcatacacga agtcgaaacc
     6121 gactccataa tggatattgt atgtcattac tgtgatgcgt ttcgacccca caagtccgcc
     6181 gtctgacgca actggcaccc cggcgccggc gcaacaactt gcgccaaaca acccataccc
     6241 gaatgccaac aaggcaatga tggaaagcac caagcccttt ctgtgtgtac aaagcaacat
     6301 gtgacccgcc cccaaaaaat acattcatga tatacagacg ccagcctgtc ggcgtcgctt
     6361 cgctcgccat acgaaactct ggcgaacagc tggataccga tgaatcttcc gtttcgggta
     6421 acgattttcc ttcccgcaag ttcgagtctt aatcaacctg ggctaatgga ttatgaaagg
     6481 cggggcccta aaagcgtggc gcatggttct ggatgctctc aactcaagca gtcccaaagc
     6541 aaatcctgtt acgtggtaat cggcaggcgc aatgccagtt tacactgttc tggcgttcac
     6601 aagcgagtca cataagaaac gcaagacagc gccgccgcga gaacctgttg tctcgcaacg
     6661 gcgccgtcat ttgtatgcag acgggcttca ctcgctgacg atcccgtacc ccggcgcata
     6721 gcctagcacg cattcacaca tacgtgcgcc ggttttccac ccggaaacgc caacccttcc
     6781 tgtctcgcac actccactta tggaaggcta cttcctccgc attatgcggt ttgcctcagc
     6841 agtgccctcc ttcaatgccg cctcaacagg cttcaagccc aaaagtgcat tctcaatggc
     6901 aggttgcatc gtgctgagta cctcttgaat atagaggtgt ggtgtttccg gtcggcagtt
     6961 cggcgcctgc tcgacgaatt gcttgatcac cggatccgtg acgtatttgg ccggtgatac
     7021 tgactttctg ggggccacaa atcctgtgtt cttcattata taggccatat tgtcaggtct
     7081 ggtgaggaaa agcacccatt ttgcggctgc atccttgttc ttcgatttgc cgagtatccc
     7141 ataagaaccc acagtgccgt aacctatctt ctttgcatcc ttaagaatag ggccggctgc
     7201 aatctcgaaa tcgaggcctt cggctctgag cttgatgacg tccatgttct cgcgctgcag
     7261 atctatgcca atcttgcctg acgtgaaggg gagtccgccc ccctgaatgt tcgacttctg
     7321 catatagccc ttgtcaataa gacctttaat gaactccact gctctcactg cctcagggct
     7381 gtcatagata gcctcagttc catcggcgct aatgagatct ccgccagcct gccagacaaa
     7441 cggaaccacg ctcgtaatcg ggctcggacg attgaggtca aatctgatgc cccactgatc
     7501 aatcttgccg tctccgtcgg tgtcgcaggt caacttgcgc gcagcctctt ccaactcagc
     7561 ccatgtctcc ggtggagcct taggatcgag tcctgccttc ttaaaaaggt ctacgttgta
     7621 tatatacgtg accacactct gaagtacggg catagcatag aggcgtccat catactgaca
     7681 tgacttgata gcgcctgaaa agtagtcagc cctaacatcg gaaggcaaat acttgtcgag
     7741 gggcgccagg ttccccgtag ctgccagttt cgggactaga aacacgttga ggtacatcac
     7801 atcaggagcc tggttcgcag ctagagctgc cattagcttc tccatacggc cctgccacgg
     7861 gagaacctct acatgcactt tgatattggg gttttctttc tcaaattcat taacgagctt
     7921 gctgtacatt tcgatgtcat cgctgcatag cgggaacgcc cacatggtca ctgtctcctg
     7981 ccgtgcagcc aatcccgtag acgcagcaaa aagcaagagg acacagattg tgagcaacgt
     8041 agcgcggagc attcttacga gctttccatg cgacttcata gtatgatatt cctccccttc
     8101 agcgctttta gacgtcagcc cttgattcca gctgacgaga ctccctcgac caagtacttc
     8161 tgcaacaaca gaaatagaat gaacgttggg ataaagcccc acactgctcc tgccatgacc
     8221 acccccattc ctcccatttc tccccttgac ccgctggcct gaagtgcaga caaccctatc
     8281 tgcagggtga acatgtctct ggtctgagtc atgatcaacg gatacaggaa atcattccac
     8341 tgcgtcacaa aattgaacgt agcaagaacc gctagtgccg gcttgcacat ggggagcagt
     8401 atcttagagt agaactgaaa tctagtgcag ccgtcgagac ttgcagcttc gtcgtaatcc
     8461 ttgggaattg gctccatgaa ttgcttgagc aaaaagatcc caaacgcgct cgaagccgtg
     8521 ggcaacagca atgccgtgta agtgtttgta agattcagtt tggaaaaaag caagaacaac
     8581 ggtactaagg tagtctgact cggtattatc agcatggaca tcatcatcca aaagagtatg
     8641 tctttgccgg gaaatgacgt cctggagaac gcataagccg caagcgaaca cgttatcagc
     8701 tgcagaattg tgcttccgcc cgccacaata aagctgttta gtgtccatcg catgatcgga
     8761 ttctgcttaa acagcatcct atagttctcc atcgatggat tctcagggat ccatctgaac
     8821 ggatactcca atacctgcga acctggctta agagactggg aaaccattgc aataaatgga
     8881 acaatcattg ttgatccgag caatactagg ataaagtagg tccagaagtt tttcatcggc
     8941 atttttctta atcgattcat cgctctcacc ctctcacctt gcccgcagca gcttgctttg
     9001 gagtatgatg attggcaata ccatgatcaa catcactact gacatagttg aagcgtatcc
     9061 taggtcaaag tattgaaaac ctgtttcata caagtagtaa accagcgtag ttgtggatct
     9121 ggcaggtcct ccatgggtca tcatgtacgg ttggacgaac atctggaacg accatattat
     9181 caacatcaca gtggaaaaaa gcgtcgtggg cctcaaaaga ggcagagtga tatgcctgaa
     9241 tacctgccat tctgtggcgc cttccactcg tgccgcctca tgaagtgcaa cgtcaatctg
     9301 ctgaaagcct gcaaggaata tgactatggt gtaacctacg ttcttccata tcgacatgag
     9361 aacgagagac ggcagagcaa gtttggggct taaaagccat ggcaccgatg cgattcctat
     9421 cttgttcaga atcgaattga gcaagccgat gtctgcgtca tatatccatt gccaaagtat
     9481 tacgacaatg acataggatg ttatcaccgg caggtagaag atcgatctga acaggttcat
     9541 ccccttgagc ttcttgttga gcacgaaagc taagagcact gatattatca ggcttggcac
     9601 tacataaagc gcacagtaat acaatgtatt tacgagcgcc tttctgaaga cggcatcatg
     9661 aaacagcctt ctgtagttat cgagccctat gaatcttggc tcggtcagcg ctgaccaaga
     9721 atagaagctc agcactacgt tggcaacgag tacactcaac ccaaacacga ggaatatgat
     9781 gacggagggg gccaaaaagt agtatgactc ccgcgatttc ctcagttccg acaatgtgtt
     9841 cacaaacctc acctcgactg tctgccggat cttgtctaaa gcctgttctc tctcctcaga
     9901 gcaagaagca acgactccct cacacccagt ggaagctgaa cgggagaatt cccccgtctt
     9961 tgcgactcat agattgccat gatggcctcc aaggctgcac gtccgtcctc ccccgtgctc
    10021 ctgcttggct ccccagtctc tacgcagtgt ataagctcgc ctattgcgtc catatataca
    10081 ttggcttctc gttcaggctt cgggaattca accttctcaa gttcccgaaa gcccgcagct
    10141 ctcttgctcg gaaccacctg ccacatctcc gcatcaggtc caaggagtat cttgccgtcc
    10201 gaaccctgaa gttctatgtc aaaccgcata tacgacgttc tctcattaac caaggcaaca
    10261 gcggatacgc cattcttgaa gcgcatatgc gagaagcagg tgtcttcgac gccatagtgc
    10321 agatgcttgc gcttttcagc actgccaact actgaatcaa tgtcccctgc gaaaaacctc
    10381 atgatatcgt agaggtgagt ggcgtcgtga agcagggggc cttctctgtc gctcagccag
    10441 tcgggagaag gtttgcaccc ttgacaattc ccaaagatgc taacgagttc cccgatggca
    10501 cccaaatcca acagctcctt agccttcacg taatagctgt tccatcgcct gttatgcatc
    10561 accatgagaa ctgtgccaga ttttcgacag gcttctatca tgtcatccgc atcatcaagg
    10621 ctcaacgaga tgggcttttc gcatagaatg ccgcgcaccc cgcactcagc ggctttgacc
    10681 actatgtcgc ggtgaagctc cgggttagtg gttacactga gcaaatccag gttctccttc
    10741 tcaagcatct cgttgaaatc cgtatacaac gcgtcgacgc cgaatcgctc gccgaagctc
    10801 ttcaagcttt tgtctgttcg tgatgaagca gcgacaatct gagtgttagg aatgctcgca
    10861 tatgcgcccg catgtgtaca cggtttggga cgattggggt catcctcaag tttccacccg
    10921 atgcggccgc atccaacgat cccagctcgg tatctcataa ctcgattctc cttcctatct
    10981 ggatgacgac aactatatga gcacaggaga atgctgctta gcagaagccc tcgcagcgct
    11041 caggattttc actatctcgc ccatctccaa cggaggtacg gggaagcaac gccgatctat
    11101 gcatttcagg aactgtctca tggtctccac aaacccgcca tatgccgaca aggcgttgcc
    11161 gatgccatcg ggcgtgccat atccttcatt acataacttg gcacacagtt cacggctgtc
    11221 tatggagtct tctgagccat tgaccatcac agagtattcg agctcgttcg ttgcgcaaac
    11281 cacatcgcag accgctattg caccatccgc aaacttgatg gtaatggctg ccgtatcctc
    11341 cgattctatg tctttgtact tgactctgct cgtctcacag tacacacttg ctgccccggg
    11401 cccaagcatc atatatccaa gctctacagc atgaacacca atgtccacaa ccataccccc
    11461 gcccaacgac agagaatcat gccatctctt tctggggtct gtagcgaaat gatgcatagt
    11521 gtgaatgacc ctagcatgag ccaaagtggg cagtcctagc cgcccctccc tcacacttcg
    11581 cgcgatctcc tggaataccg gcgaaaacct gagcggactg caggagagaa tggggcaatc
    11641 gttgctcaca gccgtgtcaa gaaggtcctt tgcctcttca taatcaaagc tcagaggctt
    11701 ttcaacatag gccggcacac cttgttcaac aaaagctttc gccagcttgc agtgatcggc
    11761 gtttatgctt tcgacaagca ccccatcaag gggcgccttc agaagctcct gccatgttgg
    11821 cgccgcttca cagccatttt ccctggcaaa agcctcggcg gcatcccggt gataatccca
    11881 cacatatgcg acatctgctc cttcagcctt gagtatccgc gcaaatgtgt atgggttcga
    11941 cagtccaagc ccgattatgc cgattcgagg tctgtcatgc gacactctgc ataccctcca
    12001 agcttcaatc tactaaatga gaccatgctc gctcagtatg cgagatagta tctccaaatc
    12061 gtccaggggt ttctggtcat cgagcggatg ggtcagttta cccgagccct cccccatcat
    12121 cttgaggcag gccttgagcg cccccacagg agaaccgcca tgcttatata cctcgcatag
    12181 cgacgagacc ccggactgca tcttgacggc tgtggctgca tcaccgcttg cagccgcgtc
    12241 gtacaggtca acgcatgctt tcggatccag gttggctatc cccggcacag ctccatgccc
    12301 gcccataagc acactggctc ctatcgacaa ttcatcgccc tggagaacac tgaatcggct
    12361 tgctgatgaa gccagcgata tgagacgttg gtgatagtcg aaatcgccgg agctatcctt
    12421 tatcccgatg atgttagggt tgtcggtaag tcgccggacc gtttcaatgc cgatccaggt
    12481 cttggtcgtc tgtggaacat tgtagagaac taccgggact tggctctttt ctgcaatgtc
    12541 ctcgtagaac ttgactagcg cttcctgttg ggttgggaag tagtaagggg tgcacacaac
    12601 tatggcggat acgccgggca gtgcagcggc ctgcatgttc tcgaagaccc tggctgtgct
    12661 ggtgtctcct actccaacaa gcaccggcat cttgccgcca acctcatcaa cagcagtctc
    12721 gatcacccgt gccttgcagg catctgtcag agccgcaaat tccccactgg agccgagaat
    12781 gaaaataccc gatgcgcctg ctcgaaccat tctccgtatg gatctgcgga atgcctgctc
    12841 atccaaggca ccatcatcat cgagaggcgt aacaagtggt ggaattatcc cttttagttc
    12901 gaagtcagct gtcatttttc cgtatcccct tcttgacgtt gagcaaagta gtagcgctgt
    12961 aaatgcctcg tcataagctc ttcacatttg ccaacatttt tctgtcgaaa agcattgtaa
    13021 atctctttgt gctcgctgag gattctggca gggctgacac cggcacctac cccctctgcg
    13081 gagatgatat ggaagaattc ctgtaaagtg tgggaagact gactcagaat tggattgtgt
    13141 gttgcttcta caattgcttg gtgaaatgcg agatcttctg ctgcacagct ttcgcctgct
    13201 tcaacttttg cttccattcg atatatggtt tctgccatag cttcatagtc gctctggtca
    13261 gccttctcca cagccaattt caaagccgcg gtctctaaca ctaggcgagc ctcattaagc
    13321 tggaaaatct tctctgtgtt gagcccggaa ttgaacgcaa ctgcatgaat caacgaggcc
    13381 acattgggct cgctcacggt aatacctacc tttggcttgg actccactat ccctaaagcc
    13441 tgcaagccca caattgcttc tctaaccgat gatctgctta cgccgagcat ttcagcgagt
    13501 tccctttcag tgggcaacct gtcgccagtt ctgaggccct tctggactat gtatcttttt
    13561 atcgcctctt caacttgctg gctcacggat ttgtgttcaa tcttctgcac aacgccctct
    13621 ccttgcagcc tcattttgga catatcatcc tatggtatta tggtcagacc acagggtcga
    13681 tgtttgtatt ctacacgcct cgcaaaactc cttctctaaa attgaaaagg ccatctgcat
    13741 caaaaagcga actctataca ctagcaaccc aaaactttca tggacatccg ccagcggtct
    13801 tccaccatgc tggtctccat agagcaggag ggaaatacac atgagcgtgc caggagtccc
    13861 caagcctgaa gtcgcagtca ggccggtcct cgacagcaag caaagggcgt tcgtcgattg
    13921 ccaacatggg tgcgtggtgc tgcacgcgcc tgtcggtacc gggaaaaccc tagcccttgc
    13981 cgagagggct gcagaagcca tacgccacgg ggttgatccc aataggatac tgtgtgtcac
    14041 gttcacgaac agggccgcag aggagcttcg ccaacggata acagtcagtt gtggagagca
    14101 tgcaagaggc gtagtggtcc gcaccttcca cagcctttgc gcgtggatgc tgaggagcga
    14161 ggcaaaacag atgagtcttc catcaggctt tgtcatcttc gatgacgacg actccatgga
    14221 gatcatcaga caatgcgcga aaaggactgg cgttcatctc gtttgctcag gctatgacga
    14281 tgaagcgaga gatgcgcagg atgcgatcac aactgctaag ataaatgctc ctgatgccgt
    14341 gctttccaca aattccgtgc aagatgcggt cttcgacagc ctgccccgag catggcgtcg
    14401 tctggccctt gcctaccagg atgaacttgc tgcccatcat gccctcgact tcgccgatct
    14461 cgtctacttt gctcgcgcta tgctttctgt cgagccggac attgccaacc gctgggccga
    14521 acgtttctcc atgatacaag tggacgaaat gcaggatacg cacgtgtcgg aatatcaggt
    14581 tctgcgtgtt ctcgcaaaga agtacgggaa tatcgcgctg gcaggcgatt tcgatcagac
    14641 catatacgaa tggaggggtt ctcagccaga gaaaatcatc acgctgctgc aaaaagactt
    14701 cccacaggct cggcgcttct catctgtcac caattatcga accacccgca cactggtgga
    14761 tgctgcggct tctgtcgccg ccaactacag tgacttcgac cgagtgcgtc cgtgcgccac
    14821 agcagaggaa ggaaacccga tagtagtcca cttcgcccgc gattctcaag ccgaagccca
    14881 atggatagca gaggagacgc gcaaactgat agaaccgggc gaaagcgcca acaacattga
    14941 cccacttcgc cccggaagaa tcgccgtgct aaccagaacg aactggcgcg gaacagtgat
    15001 ctcggaggct ttcgcagagg ccggcattcc ccacctcacc gtggagcaat ttgaattctt
    15061 ccgcaggcaa gaggtgaaag acgccattgc ctacctgagg tttctgcgaa acccattcga
    15121 cggtcgaagc tttcagcgga tgcttcttag gcctccccga aacatcggac gccaaaccat
    15181 cgatagtgtc atagaagccg aaagaacagg cctgaggctg atcgacatgg tcaatccatc
    15241 cactatcgag ttgggagacc cgttcgcacg gcttatgagg gaactcgatt ccggatctgt
    15301 cgtagtcttc gataccgaaa ctaccgggct gaaccccgcc tccgacgata tcgtagaact
    15361 agccgctatc cgcctcgaac atggacaacc agtcgccaca ttccaccgct acctgcgcaa
    15421 tcgcgtgagc gtgggcgatt ccaggcacat tcatgggctt tcagaccagt ttcttgcagc
    15481 ccacggagca gaccccttgg agaccctgtc gcagttcatg gcctttgcag atggcgcact
    15541 gctcgcgggc cacaacgtca gtttcgacgt acgcatgttt cgggctaatg ctcgacgcct
    15601 tggcctcaac ccaccatcgc accagtgtgc cgacaccctc gacattgcgc ggcggttcct
    15661 ggacgttgac gattattccc tggccaacct gacacgctat ttcaacatcc cctgcaaacc
    15721 tacgcacaga gcgatggacg atgtaaacgc cacaacggct ctcctcatcg ccctagcccc
    15781 gtatgtaaac aaaggcagta tcggcagaat gcaggctgtc cgggaaacgg gcgaatcgtt
    15841 tgtgcccctt gcaagagaga tcgaggaaat gttgatgtta gcagaaagag ttcgtcccga
    15901 cgctctcctc gacgaagtgc tggagaggtc tggtttgaat gcctactaca tcaaagagcc
    15961 ccgccgcatg gaaaacctga gagagcttgt gaggatcttt cgagacaagg atgacgtacg
    16021 cctcgatcct atctcttcct tagagagcat actccacttc gtcgccttgg ctcggaacgt
    16081 ggacagggtt gatccagaag acgagcgcgt ccgaattcta acggtccacc aatccaaggg
    16141 tctggagttc gatgtagtgt tcgtagcagg gctgtcggag catgaattcc caggctatcg
    16201 atcggtaaaa gacgggcgac aggacgaaga gctgaggctc ttctatgtag cacttacacg
    16261 ggcgcggagc agactcttct taaccggaca cgccgtaaac aacggcaagc atcgagaacc
    16321 cagcccctac tttggtacca tcagcgatgg atgggtcgaa caggattcgc atgcaataag
    16381 acgaacatac gggtccggca atagaagaaa tccacaatcc ttacagagta agggacacag
    16441 gcgcccattc taaacagatc tcaagtaacc gcgtggcgtg agtggagaca ggagagcttc
    16501 agtcgcagcg gccagttagc ggcgctttgc acatgcaaac agcatgagca ttgccgaagt
    16561 tcaccgcagc gcagccgaac aagcccctcc tccaaaggct ccataatctg cattcatgcg
    16621 caattcgcct cctcatatct tcccgcccat tcactgaata gagcttttgc catgacatga
    16681 tttcctgtag aacaagaccg atttggccag cgcatgccag gtgagaggag ctagggcatc
    16741 gtttccctct tcgtttcgcc aggccggggt gacccactag ccggtcatcc ggtgaacgct
    16801 tgggccttcc cccgccttca tgtcccacga tcgcattggc atatttaaat cgccgacaaa
    16861 cctgacagca cagaactcga agcgactggc aatatgcgcg aatcggaagg ctcatgattc
    16921 ggctaagcct tgcgcgaacc ttatcgcgtc acagtgtctg ccttttcttc acccatgagc
    16981 gatacagtag actggttaac tgggcgctca gggataatac gtctgaaaca cgctaagttc
    17041 ggaatcttag ttcccattgt catattcttc gaaaaagact tttcctttac acgactatcc
    17101 tcataatcca cagatttctc gccgtagccg gactgaatat cacgagagga cgtctccccg
    17161 ctttccttga atctgattgt taactagccg ctgattcttt ggaatgcaag ggaggcacgg
    17221 aaatgaggcg gcgcacgttt gtggccatga tcgtccttat tggactcttt gcatccccct
    17281 acgcgctcag cgcggaggaa cagcagcacc tgatgccggc aaccatcatt cgtgtgctag
    17341 atggagacac ggtagaagtg attctggaga acggcgcacg gcagagagtg cggctgatag
    17401 gagtggactg ccctgagttg accgacaaag acccctcggt ccgtgattat gcattcgcgg
    17461 cagccgcctt caccagagaa ctgctggaag gaaggagagt ctggctcgaa actgatatgg
    17521 gcacactcga caaatacggt cgactgctcg catatgtatg gactgcaccg ccagccgact
    17581 ttgcggagga aaagataaga gactgcatgt tcaacgccat tttgctcctg gaaggctacg
    17641 cgtacacata catgcttatg cccaatacaa agtatgcagc ggttttcgtc ggttttcagc
    17701 atgaggccac catggaaggc cgaggaatct ggggaaccat gagaagatgg attcacatca
    17761 tcgtatatgt gaccaataca ggcaagaaat atcatggcga cggctgccga tacctcgctt
    17821 caagccgcat tcccatgacc ctcgccgaag ccatgtggcg cagatatgag cggtgccaga
    17881 catgcaaacc gccatactgg atgtcggctg gaatatccct tgaatgacgt gctcgtgagg
    17941 attgctctgc cgcgcggcag agcaatcctc tccgaaacct ttttgtgctc caggaatctc
    18001 tggtgcgccc tatccgcaga cgataaaaaa gtctatctct ttgtgccaag gaaaaacagg
    18061 gcaatagttc ctggccccga atgggcgcct atcaccggat caacatagtt aatcctgaca
    18121 tcccgcacgt tcagacgctc cctcacgagc ttctcaacgc actcggcatc ctccagtgaa
    18181 tcgccgtggg atatgaaaac catctgctcc gtagggtcta tgcaagtctt ctccatctca
    18241 tccacgaggg ccactagcga cttcttccta ccccgaacat tccttacagg aacaagcttt
    18301 ccttcgtcgt caacatgcat cacgggcttt accgacaaga ccgtccctat cactgcagct
    18361 gagacagata ctcttccgcc ccgcttcaag tacatcaagt catccacagt aaaccaatgg
    18421 caaagtcgaa gcctgttctc gaggagccag tccttaacct catcaatcga tcttccggca
    18481 agcctttgct ctacagcatg ataaaccaat aatccctgac ccattgacgc agcaagagag
    18541 tcaattgcat aaatcttgcg ttgaggataa atctccctca tattgtccgc tactatggac
    18601 gcggcgttgt atgtcccgct gagcgccgat gaaaaaccga tgtagagcaa gtccttgcct
    18661 tcctgtagaa cgctttcgaa tgcattctcg caatccctca tgtttatgag ggaagtctcg
    18721 gccacctcgc cttgtctcag tttctcatag aactgcttga gatcggtctt ctcgcccttt
    18781 acatagctct ggcattcctc ttccccgatt ctatatgaca gcgaaatgat atcaatcccg
    18841 tacttatcaa tcatctcatc cgtgaggttt gccgatgaat ccgtcactat cccgaagcta
    18901 ataccattcg cctcctctcc cttgatttgc ccgaaatgcc aagcctccac acgctgccac
    18961 gaggtgtctg accagcaatg caggaaacgc gaagccatcg gttctccgtc ggcatcgaaa
    19021 tgcagaaccc gatcaccggc aaggcaagaa ccaatggctt ctatcaaatc acattatttg
    19081 cagcatattc gcgccaattt catccggaca gaaaatacct ggaattaccc gccgaaatga
    19141 cgcatattac tcagcgagga gcccgatcag aacatccgga tgattagtta ttatcccgtc
    19201 cacacccagt tgtatcattt tggtcatgtc ctttgttttg tttacagtcc agacaaatac
    19261 cccaagccca ttctctcgag ccataccaac cagctccggc gtgactcccc cctggtcaac
    19321 cgccacgaag tccgcattga catcgagtgc tgtggagata accttctcgc cgaaagatga
    19381 ctttccaaaa agcaatggcg cgctgaacag aacgccggcc ggtacttgag gcaataggtt
    19441 gcggcaacgc ctcactgctt cgacgtcgaa ggactgtatt atgacatcgt cctccgtttt
    19501 ggtctctctt atgatctcca caactttgtc ctctatgccc ttcgccttta tctcaatcaa
    19561 taccttagta cgccctttga acgtcgaaag cacctcgtgc aacgtgggaa ccttctcccc
    19621 tgcgaattca gctcccttcc aggaccctgc atccagcgtc ttgatctcct caagggtgcg
    19681 ccaaacaacg gaccccctga cgctcccagt tgtggtccgg tcaagcactg catcgtggat
    19741 caccacaatc tggccatctt ttgttgcatg aacatcgagc tcgatcatgt cagcgcccat
    19801 ctcgactgcg cgctcaaagg cagccatcgt gttttcagga gctacccctg acgctcctct
    19861 gtgcgcaata accaaaggac gcttggccgt agcttccgct ttcggatccg aaaccatttc
    19921 cttctcgtca acacctatct cagtcggatt tccaggcccc gcctcaccca ccgcgctatt
    19981 cccgtgcgcc gttgtggcaa aagcaatcat aagcactgcc aagaaaactg cgaacaacac
    20041 acttcgtcgc cgatgtccca ttcacattct cctttccgcc tcaaattctg aggaggtaat
    20101 ttggcaaaca tgtgccatga ttccagcact agtatacaag aaccagacta atattgatat
    20161 actgccgcac aattacggaa ctccatcagt agcatgcagc atcggcacaa attagcaaga
    20221 ggctaagccg aaattttagc aataaataca cgttcgctgg tctgccacgc cttcttgcca
    20281 aaactaaact gcagtatgca ccagagcgaa gcaaaagatc ggacagtgta tgtcgattat
    20341 atcgttaatt tcaggcgata ttgccgttgg ctagtttctt gatccctcat gctgcctgct
    20401 catgtgccac aagggatttc tcgcgccatg tgccccgacg ataccagaga tagttgcaga
    20461 cagctcccgc gatccagctt atgaggagcg agaagaacag cgcgtcagga gatccagcgg
    20521 gatgatctgg gctcctggtc agcgatgccc agatatatgc gaggggcacg cggactacca
    20581 caacagtaaa catcgaaatc cacatggatg gcatggtgtc gccggcaccg cgcatgattc
    20641 cgccaaaaac ctgagaaatg gccatggcaa cataacccgc agccagtatg cgaatctgcc
    20701 gcacaccaag gttaatgatc tcctccgttg tagtgaacat gcgtatcagg ttctcgccga
    20761 acagcaacaa acaagccacc agcacaaatg aagtggtcag actcaccttc aggatatcct
    20821 ttgtcccctg ggcaacgcgg tccatcttat ccgcgccgat attctggcca acaaaggtgg
    20881 ctatcgccat gccgaaggtg aagttgggca tcatcgcaaa gccgtcaatg cgcatgaccg
    20941 cagtattgca cgtgaccacc tggtaaccca tgctgttggc aagggcctgc acaaatacca
    21001 tggacatcga gaaaaccgcc tgcgtgatgc ctgccggcag gcccaatcgc agcaattgac
    21061 cggtcagctt cttgtcggga accaaattgg aagcgttgag gttcacaaca tctttcattc
    21121 tccggagctt cgcaatgcat agcactgcgg agacggcctg ggatatgata gtcgcccacg
    21181 ctgcgcctgc aactcccatg cccaatccgg ctacaaacca tatgtccatg aatatgttga
    21241 gaatcgatgc caacagcagt gtcagcaatg ggaaaacgga attgcccagt ccgcgcagga
    21301 tgccggagac tatattgtaa tagccgactc ccagaatccc cgaaagcgtt atggttagat
    21361 aggagtaggc catgtcaaat gtttctactg gggttttggt caatgtaagc agctgtcccg
    21421 ccagaggtat gccaaccgcc ataatcacaa gggatgatat gaaaaccaat gtcatcgcat
    21481 tcccaattga acgcgaaagc gattctttat ccttggcccc atagtattgc gcgaccatga
    21541 tccctgcgcc cgttgaaact gccatgaaga gaacgaggag caagtttatg acggggagtg
    21601 tcgttccaat tgatgagagc gctttgtcgc ccacatagcg accgacgatt attgaatcca
    21661 cagtactgta catctgctga gcaaagttgc cgatgagcaa ggggatcgaa aaccgcagca
    21721 agacggacat tggctttccc tcggtcatat cttgtgcgcc aaacaatgcg ttgattctct
    21781 tgagcatgta gcaaaccacc ttttctagac gattatgcca cgtgaccgcc atttgcgcaa
    21841 tccggtaaca gatagcgata tggaaggtat ttatgggaaa tgcgggtgat gatatgcctg
    21901 cgcccgggct acatggcctc ggaacgcagg caataaggaa gcaaaaagac atggaataag
    21961 ccaacgatca atcgtacatt accaccctgg gcctaaggtc aagtcttttc atcatgtcat
    22021 cataatcctc atcggtgatt tcatcaatcg gcttgtcgat caaggcaatg agcatggcct
    22081 ccatcaggtt tgcaccaaac gaacgtccct gtatctcagg gctggtggtg acgagggcat
    22141 gagcgccacg ccttcctatg tcctcaacat cgtcttcagt caccgtattg gtgacaataa
    22201 tcttcccatc gatccgctca ggcaggtgcc tgtatatata gtgaaaatct cctgcgagca
    22261 cgtgggctct gttgtagtat tgctggaatt tgagtttgcg cttcttcgcc ttgtccctgt
    22321 tggcccgttg ggacttgcct gtaggatata cccagctgaa tgggagttga gtcagccacg
    22381 gcataacaat aggggctagc gcgtggaccg aactgatctt gtacagcggt ataggtattc
    22441 ccgtcgcaaa catcaggtct ccataggtca tgcgacaccc aagttcctcg aaggcaagtc
    22501 ccattccgat gcggtcgaac acacagacca ggaagacgtc ctttcctgct atgggcaagc
    22561 cccactcggt agcgacagac atcacagcat tgtactccac cctgctcttc agcccagttc
    22621 catctacgac aggggtatct ccggccatgg cagcaatatt cttagcttct cgaaagtaat
    22681 atttcctgcc ttttgcttcg agatacaggt cgatcccgcc aagtccgaaa gcatcaactt
    22741 tatccttaag ctctccaata agctgtttgg cttttgcgac atcaccgtct gttcctatcc
    22801 gctctacaag aatttcctct cccaacaacg taatcctggc tgagtggttt cgagaagatg
    22861 agccgatgct tacactaaca acatgcttca tgattccatt ctccctcctt caaccggctg
    22921 aagcgtgcgt atcccccccc caataatcag tctattcgcc ctgtatggcg gaccatgtgc
    22981 ctgtaactga acacaccgcc ccttccacac agactcgtct tcgtcctaaa tgaggctgca
    23041 caggaaatgg acgttgtaga accctgaaaa cctgcgtagc tccgaaaaag catttgggct
    23101 ttttcaagta gctgagaact gaacagcaag caacgctaat gtggacaagc cgatcagaag
    23161 ttgctcagcg caaggcagct cagatgggga tgatcagtag cacataacgc agcggtcatt
    23221 gtctaaaccg acatctaaat ggtgtacaca tatttgtgca catattgatt ctaccaaata
    23281 ggctttggag accgcaagat aattggccca ttggtgatct aagccgcgag gagctggcgc
    23341 cagtcgccct agacgatcga ctcacggtca attttcttcg ggcgtggcta gcctgcagac
    23401 agtcattctt cccacgaagt gttgcagatt accaaagaac cttacatttc gtgttgctag
    23461 ttgaaggttt ttctgccatg ccggagaagg atgagcaatg gcatgatttt gctcctaaat
    23521 ctgttcatgg gattcttctg ccattatcac tctttccaag caattcggag gtgtgatctg
    23581 acgtgtggaa tgagctagcg gtcgaggcaa tcacgatact attgtgggct gcgacagccc
    23641 ttgttgtgtt gttcttgagc ggcctttcca agtaccttca ggagcaagca aggcgggtgg
    23701 agaatgatgc agcgcgagga ctaatccagt cagcgctctc ggaagcagaa aaggttggca
    23761 ttgatgccgt tcaggcaaca aaccaagtat tcgtcgatgc cataaaggca tcttctgcag
    23821 acggcaagct ttcaaaagaa gatgcagcta ccgcaatgaa aatagctgtt gagtatttca
    23881 aaacccacct gtcatcccga agctaccagg ttctcagcga acccgatggc aagctcgacg
    23941 ataaatggat aagcgacttc ttggaagcaa gattggcgca gacaaaagca gaatcctctc
    24001 gctgtgctta tccccttccc gcaccgttac ctgattgtcc cgagtaacaa ggtcaaggct
    24061 agggggcggc aggcacactg tcgcctcgtc tgccccgggc tcggctacgg ctgccaatgc
    24121 acaagacccg gagaggccat ggtcaccaag ggagcgatag ctcgacgcct agttcaccgg
    24181 attacaaacc aagtccccta tctgcaggag ctgacggatg acgggaccga gactacaacc
    24241 ggcctggccc tgcgcaacag gatgctctgt gattaagcaa gccctgtacg cagggcccga
    24301 agctgaaaga tcttagcctc tcaatattca agggtaggaa gaaaggcggg catttgcgca
    24361 gatgcagata ttgtcttgcc ttttatagca cgtctctggc tggaatggga aaacgcagaa
    24421 gcaaagaaac agggggcggg atgcccgtcc cctcagttct gcgctcgcct ctgtccggtt
    24481 ttccgcttct acagatcttc cggcccaatg gtgtatgtcg gaggcattgt tggtgtccaa
    24541 aacatgttcg ggggcacagg atagactgag aagccgccct cagccgtgcg ctccaggttc
    24601 tctaccagct tctctctgac caacgggtat ggcgccacaa acactacttc atgggtctgc
    24661 gttcctccga agatcctgtc accggcgcac gggaatccaa ctaccggctt gtcgtttgcc
    24721 actgcatagc cgatgcagga gcaaagggcc gcttccgcca cggtgtctgc agtaatcttc
    24781 tcgccagtct cgtatgcaca tgcatggatc aacctcatag cctgagctgg attgacatac
    24841 actacgacca catcaggatc ctcttccatg ctctcgagag cccctacgag aatcccatcg
    24901 tatctcttgc cggtatcccc tatcttgaag gtgttgtcca tgaaggtctt gcctgctgtc
    24961 tgatccttca cgtatgcgcc gacagcagcc ttgcctgatg agatggcttc aggggtgtca
    25021 ataagcccta cacaggccgc gcccatggca caaatcatct tgtccggtgt accggagttc
    25081 gtctttccct ggtaacgggc catggcaacc aactggcaca agtttagctt gaagttaaaa
    25141 ggcttcctcg ggagatcctc ctgatttttg tatagtttga tccccactgg cttggtgtca
    25201 agacgtaaaa tcctatcgag ttccgctgca acttgcttct tctccatgta aaatgtctcc
    25261 ttccataaaa actgcagaca tttgcctgta cactcaatct cgaatcgctc aatcgcgacc
    25321 ttacatatat cattatgcgc ctgattgtga aaaatatcac gttatctcac aggcaacagg
    25381 tcccgctcgg ccaacatgcg cggtatcgta tccaccagag ggcttgttta gacatgggta
    25441 gtgaaagaac aggcgattgc ggaagtcgat catacagtag ttaatatggg caccgcttat
    25501 ggaaggcatt accggagtcc cctgcaaatc ccgtggtcac atgagcttct ttctcaatca
    25561 gaaaggcaga ccgcagccag cttatattta ctcctagatt gcccgctgcg gtcgatgtat
    25621 ttttcgtaag ctacttccaa gaagccttca ctgcttcaac gagctccacc gtacgtttgc
    25681 tcacctcttc cggcaacacc ttcaccctgg cgactcctga ggcctgtgcc gctttggcca
    25741 cgttggcggc aacggcagga gcaactctgg gatcgaaggg accggggata atgtactctg
    25801 gagagagttc gtcttcggta acgatatcgc tgatggcgta taccgccgcc atcttcatct
    25861 cttcattgat agcactcgct cgggcatcaa gcgcgcctct gaacactccg gggaaagcca
    25921 aaacattatt gatctggttc gggaagtcag acctgcctgt ggcgatgacc ttagctccgc
    25981 cagccttagc ctcatcaggc atgatctcgg gaacggggtt agccatcgcg catacgatgg
    26041 cgtccttggc catgctggcg accatctcac gggtgacaca tcccgcaacc gaaagaccga
    26101 tgaatacatc cgcgcccaca attatatccg ccagactccc tgacaacttg ttcgggttgg
    26161 ttatcgcggc aatctcttcc ttataagggt tcatgccgta ggtcctgcct tcgaagatgg
    26221 cgcccttgga gtcgcacaac acgacatccc tcacgcccag cgccataagg agcttcgtaa
    26281 cagcaattcc agccgctccg gcaccgttca ctatgactcg aacatcagat gcgttcttcg
    26341 acacaatctt cagcgcgttg atcatggcag cacatgttat tatagccgtg ccatgctgat
    26401 catcgtggaa gaccgggatg tcaagtgccg cttttaactt gtcctcaacc tcaaagcacg
    26461 tcggggccga gatatcctcc aagttgatgc ctccaaaggt gggctcgagg catttcacaa
    26521 cattcacaat ctcgtcgggg tcctttgtat caagacatat ggggaatgca tctacgcctg
    26581 caaaagactt gaatagaact gctttgcctt ccatgacggg catgccggct gccggaccga
    26641 tatcgcccag tcccaacaca gctgtgccgt tcgtgactac tgctaccatg ttgcctttgt
    26701 tcgtgtaatc gtagaccttg tcgggctcgg catggatctc cttacacggc tccgccacac
    26761 cgggagtgta ggcgagactg aggtcctcct tggtgcgcaa aggcgtcttg ctgactacct
    26821 ctatctttcc cctgttggct ctgtgaagct ccagtgcttg ctctctcaat gtcaagtggg
    26881 taatcctcct ttgctgtgtc ctcgcttcca gtctagcatg tatacgactg gtcgtcagta
    26941 catcgcgcct aacgtctttc tgcaacatct gctgcgaatc cttcgtaagt gcacacagcc
    27001 cggaagaagg aatcgcctgg tgcctcaagc cgcattacag cgcttgccgc gtcctggcta
    27061 cccacttccg ggccgtagta gttctcaagt tcggcacatt acttaatgac tctatcccag
    27121 tcttgcgcaa aacctactat gctcttgtca gtaacagggt gctggataag cttgggcaag
    27181 accttgtagg ggcaagcagc ccaatgcgcg ccggcttctg ttgcttcgat gtagtgagac
    27241 acgcccctga tgcttgcggc gacgatttcc gtcttgtaac cgccattttt ggccatgttt
    27301 gcagctgatc gcacaacgcc cacaccatct ccgcccacat cgtccgccct tcccatgtag
    27361 atggagataa agtctgctcc cgcttgaata gcaaagaagg attggggaat cgacactatc
    27421 ccggtcaggt tgaccttaag tccacggtcc tgcagcacct gcatcgcagc caatgactcc
    27481 tgggagatgc acatcttaac gatcaaacga tcacccagaa cactagcaaa ctcctcagct
    27541 tgcgcaatga tgccgtcacg cttgtcctcg gtaatctgca aatggaaggg accttcaatg
    27601 atcttggcgg catcgctcac cgcttctttc cacggtttcc tcacttcggc cactatggta
    27661 gggttcgtga ttatgccatg aactccgtag tcctttgctc cgattaggtc cttctcatct
    27721 atgctagcta tcaatatctt caatgtagta gtctcccttc agctgccatg atacactcgc
    27781 acttaacaat gcattccatc attctctggt tcttgatgga cagtgctgcc ttgcgcctac
    27841 caaaacccta tagtgctcca tctgccgagt ccaccagcgt tctggcggtt atctcatctg
    27901 ttacaagcat gttggcgaag cctccacgga gcgccccggc aatcccggga atctttttcg
    27961 gaccgcccgc caccagtatg cagctctcct tttgggtcaa ctcgtcaagt tcaagcccaa
    28021 tagtcctgtc attgagggac tcatcgcaaa cggatccatc tgcagtaaag aacctggagc
    28081 aaatgtctcc gacggcgccc ttggataaca ggacgtcgat atcttccgcg gaaagatagc
    28141 ccgcctgagc caagatacaa gacttgcttg gcctccctac agagaagata gcaatatttg
    28201 cttgtctacc cttctcaaga attcttctga gattagggtc gcgggctagg gcgtctctga
    28261 gttccctact ttcaacaatt gttggagcag ataagaagta cggcggcgca tcaaatgcag
    28321 cagcaacttt ggccactatc tcatttccgt ggattagtgc ctcgcccttc ccagaaagcg
    28381 caccctccag ctgcacaatc ttgacgtttt cgcgttttat gggcaaaggt ttgagcgctc
    28441 tggccatctc cagcatggtc gtgcccccgg tcaccccgat cacgtctcca tctcggatta
    28501 tctcctttag gagacgtgca gcagcctgcc cgaggtgcca tctaagaggc ttggggcttg
    28561 attcagagat tgggcatacc actactctct tcagaccgta catggattcc aattccctac
    28621 tgagcccatg ttcctcctgg aattcatcga cgatagtcac ctgaacaatg ccgacttccc
    28681 gggcccgagt cagcagcctt gagatgcggc tccgggacag gccaactcgt tccgcgatct
    28741 cctgctggtt aaggtcatgt tcgtagtaca tcctcgctac ccgaaccaaa agttcgtgct
    28801 cactgctggg attctgttca atttccacga cgtttctcac ttcccaatgg cataaccaat
    28861 actaacaagt tatctgtcaa ccgagacaga cacgcgcatg ctggcacatc tatgattgcc
    28921 tatgcacatt cttgcactcc agaggccttt tctccattgc acaagagact ccttcagccg
    28981 caaggtgtta accttgggaa gcaatgcaga cccgttgaca gtgctcatat cctccttcga
    29041 aagcacgcaa acaacccggt acgcgactga ttctgtcact acagccagga ctaatcccgg
    29101 accttgcgac cgacaaaatg tctttgagcg ttgtacatgt ttatgtcgcc gatttcagag
    29161 agttcttcca ttacttcctc caaaagctcc aggttgggaa cctcccagaa ggtgaaatac
    29221 ttccactctg tggaccaaca acagtcatac accccgtgca tcttgacgcc cttttcccgt
    29281 gcacggctga agatgtcctc tacttttctg tcgaattctc gcttttcatc ctccgatgca
    29341 ctgtaccacg tgtcggtcgc tttccatgcc gcaaaaaatc ccaatggttt ctcttctgcc
    29401 attacggtca actccagttc aaacgggcta agccacaccc agtttaattt tcatattcga
    29461 gctcgacctt ttcgtccagt tccttcatga taatattgtc aggcgcggtt ttgtgtttga
    29521 acacaacgaa gaacgggcct tcatcctcaa ccggataggg cgacagatgc cacacaccct
    29581 tatcgtaaaa caccgcctgc cctactttga ccaagaagcc tcttacattg cgcgccaagg
    29641 gctgagcatc cttgtttgcc ctgtcgtctt ctgacgctac aggaattatc aggtcgctct
    29701 tgatcggaac gagcaactca taggttgtgt tgtgccgttc caagccttcc acaatgagtg
    29761 gacgcttctt gcatactcca tgcgctattt cgagggtttc gtgatcaagc tcccatttga
    29821 ccaaaccggg ccagaggtcc tcgacggccg acactttttt tgcgccttca ggcatctcca
    29881 cgacttgtcc gtagggagca aaagcttcct tcgttaattc ctcgactttg actttgtgca
    29941 tgacttcttc aactccaata ccgtcagttg ctgccttttt tgcggggttg catggcggaa
    30001 ctgaacctgt tccgccatgc ggccttcact cgtgtcggaa cgctcttatt tcgctgggta
    30061 caacaccttc tgaatctcag gcctattcat gttttccttg gtaactacgg taatcggaac
    30121 ataaacatcc ttgggcacct tcttgccctg cttcgctgcg ataatatatt caacaccctt
    30181 gtagcccatt tcaaacggct gctgcactac gagtccggct atctcgccct tcttcagagc
    30241 ttcgatctcc tccgggcttg catcgtatgc aacaaccttt accttgccag caagtcccct
    30301 attcctcagc gcctgaatac agccgattgc ggcgcggtca aatgctgcaa atacgccctt
    30361 aatgtcgggg ttggcgttga gtatcctctc catgtttgcc attgccactg cctgatcttc
    30421 tccacgctgg atctcaagaa gttggacctt gggatacttc tttgccactg ctaccgctgc
    30481 ttcaccgcgt atgtcgttgc tagggacgcc tgcagggcag ttgagtatca ctaccttgcc
    30541 ttcgccgcct atctccttca ttagcaattc cattgcgatg cggccgcctt ctctgttgtc
    30601 tgttccgacg tacctcgttt ttccgtccca gttgagattg gcgtcaatgc tgaccagagg
    30661 aattccagcc ttcatcgcct gctttgccgg tccaaccaga gcatctgaat cgaggggcgt
    30721 ctacaggatg ccatccactc ttctgattat caggttttct acggcagaga tctgctctgc
    30781 aacgtcgtta gtgcttttcg ggcccgtgta aatgagtttg cccccaactt tggcaagagc
    30841 agcctctgcg cccgccttaa cgccaagcca gtactcgctc gacgtagaca tgacgaccag
    30901 cccaattact aactcctttt ccttggcggc acaaatccca ataggacaag ttatcgcgaa
    30961 tactagaacc agtgctaatg ccgacaacag tgttcgtttc atcgtttcct ctcctatcgt
    31021 ttgatgttgg cgaattcgag ctcgtcgttg cctgtagatg tcatcatttt ggggagcaat
    31081 accggtcgcc cggtcccatc acctccgctc tctcagcttc gtctgctgca atagatcgag
    31141 atacaccgcc atgatcacaa caaacccaat ggctatctcc tgatagaatg aactcaaccc
    31201 tagcagcaca aatccgttgt ccagcacacc catgatgagc gcccctatca tggtgccgaa
    31261 tatgttgccg acgcctccgg taaggctcgt gcccccaata acagcggctg ccaccgagtt
    31321 catgttgtaa tccgcaccgg ccgtggggtg cgcaagtcca agtctgccca tgagtacgat
    31381 gcccgccagt ccggccaaaa gcccagacac gaggtatacc atcctcacag tccgtcccgt
    31441 atctattccc gatagcctcg tggcctccat attgcttcca accgcatagg tataccttcc
    31501 aaaaagcgta tgattgagaa cccagttgaa gattatcgcc gcgacaatga atatcagaac
    31561 cggaacagga agaccgacga tctctccccg tccaaaccag gagagcgctt ccggcagcct
    31621 cgaaatcggc gttccgttag tcatgactag cgcgattccc ctggcaatcg acatagttcc
    31681 caacgtcacg atgaacggag gaaccctcat tttcgttatg gccagaccat taacatatcc
    31741 aaaagcagcg ctaaacgcca atgctgtaac aagacagatc caaagcggaa cgccggaagt
    31801 catcatggac gccgtcagga tatttgacaa agccaagtta gcgccgactg ataggtctat
    31861 ccctccggta atgatgacaa aagtctgccc tatcgcaata atggcaataa ctgcagtctg
    31921 tatagcgatt gtcttcaagt tcaatgccgt caggaaataa ggactgatta tagtcaggac
    31981 gatcatgcat agcgcaagga ttgccagagc tccatatttt tctatgaatg tcttgtagtc
    32041 gaaaccctgt gtcttctcca ttctctcatc agcctccaaa ggactattca acgagcattc
    32101 tcacagctaa tgccgaagtc agtttcttca tcgctatcct aaagtcgcca gtttcatgat
    32161 cttctcctga gtcgcctctt cgcggtcgag tattcccgtc aatctgccct cgcgcatcac
    32221 agcaatccta tcgctcattg ccaacacttc cggcagatcc gacgagacca tgatgatcgc
    32281 cttcccctgc cccgccagtt cattgatgaa ctcatatatc tccaccttgg atccaacatc
    32341 aatgccgcgg gtgggctcgt ccaagatgat tatcttggca tcacgacaaa accacctggc
    32401 caaaaggacc ttttgctggt ttccgccact cagtgatcga gtgacagtat ctattgaagg
    32461 cgctttgatg ttgaacatgg agaaaagctc ttcggatcta cttctttctg cctgatggtc
    32521 aatgactgaa tttctggaga actgatccat agatgcgagg gtgatgttct gatagatggg
    32581 aaaattgagg attgcgccca ggttctttcg gtcttcagtg agaaaggcta ttccattctg
    32641 aacagccagt ttcggcgaac tgacctttgc ttccttgcca tcgacataca cacgtcccga
    32701 aacatctccg tcagccccgt aaatcgcccg catgagctcg gtccttcctg agccgatcaa
    32761 tccggagaag cccaggactt ctccctcatg aactgagaat gatatgtcat gcagcacgtt
    32821 cttcctgctc aagttctcta ccctaagggc ctccttgccc ttctttgcaa cgcatttcgg
    32881 gaagaactgt gtgatctctc tcccaaccat catctgtatg agatcatctg tatcagcttc
    32941 tttgatgtcc cgagttccta tatattgccc atccctgaga acggtgactc tatcgccgat
    33001 ctccttcact tcattaagcc tgtgagagat gtatattatc gaaacatttt cctcgctgag
    33061 aagccgtatc tgcctgaaca gctcaccaat ttccttttcg gtcagcgacg ctgtcggctc
    33121 atccatgata atgatcctcg aatccgccga tatcgcgcga gcaatctcca ccatctgctt
    33181 ttgcgctgta gtaagtctcc gcgccttttg cgtgggtgat atgttcgcat tcagcttttt
    33241 caatgccttg gcggcttcag agttcatctt ctcccagtcg atatgtttag gccagccctt
    33301 gaggtgttcc cggtttatga agatattctc ggccaccgta agatctggga tcatgttgag
    33361 ctcctggtat atcatgctta ttcccagttt ggaagcatcg ttagtggatg agtaatccac
    33421 cttttgtcca cccaatatta tctcgcccgc atccttccga tagactcccg cgaggatctt
    33481 gacaagagtg gactttccag ctccgttttc gcccaaaagc acgtgcacct ctcctggttt
    33541 cacctttaag gtcacatctg atagggcttg gacaccaggg aatctcttgt ctatgtggtt
    33601 catctctaac atgtattcca tttactttcc ctcgtttttc ggtttggtga caagacattt
    33661 gctgaatatg gagatcttca tctcgtgcac ccatccctca aaatgatcgt ggcccatatt
    33721 gatcaacgaa acttgcttac tttatcgacc aattccttga cgcgatccac ctgaacacgg
    33781 ccccaccaaa caccgttctc cttcaggcta gtaccgacga tagcaccgtc tacgagcggc
    33841 ataatgtcct cggcattctc cacacaaaga ccgcttcctg ttatcacagg aaggctggtt
    33901 cccgcccgaa ccgcttcgat cttctcgaga ggcgcagccg atccagtttt ggtgcctgta
    33961 acgatgatca cgtcagcgtc gtagtattcc gcgtcccagg cctggtccga aatggaacga
    34021 tctgcaacca acgcatgcgc gccatgcttc acattcacat ctgcaagtat cttgacgttc
    34081 tctgcgccga tattctttct atagcggagg atgttcccac tgtccccttc gattatccca
    34141 gagttggaca cataagcatt tacccactca ttgacgcgga tgaacgccgc tccggaagct
    34201 tttgctacgg ccatagccgg tatggcagcg ttccaattga gaataacccc gaaaggtatc
    34261 tctattgcat cacgcacagc acaagctact gcagtcatcg ttgctacggt ctcaaaccca
    34321 acatctccgg gagccaagaa agggtaatct cccgaattct caaggattat cccgtcgatc
    34381 cctccttctt cgtatgctag cgcttcctct acagcatggt tgattgcaac cgcgagatct
    34441 tcacctttgt atctaggaga accgggaaaa ggtaaacaat gaatagtgcc tatcaatggt
    34501 ttgggacgct caaatatctc tgatagtgca ttagcctttg gcggcacagc gctcatgcgg
    34561 accacatacc tttcatcgat cagtgttatc tgttacatgt tttgctatca aaaaaggacc
    34621 ttgtgacggc ctctgtcggc aatcctgttc tcgcccctac atgttgaatc gacagtgctg
    34681 ctgcggcatt tgcataattg acagccttca caaggtccca acctttaact aaacccgaca
    34741 aaaatactgc attgaagcaa tccccggcac ctgtggtgtc aactaccggt accttgaagg
    34801 cagggacatg aaaatggtca tcccctgaaa aaacatctac cccgtctgcg ccacttgtta
    34861 tgaccacaat ttgggggcca tagtccagca aagtcttagc gcccttacag atgtcctcgc
    34921 atctgactaa agccttcaac cctgcttcgt tcagagaaac gatatgcagt ttcctcagga
    34981 cgtcttccca aaaatccaac ccaccactat cggcagtagg ctcaatatcg atggatactc
    35041 tcacgtcatt gtttacgctt gatgcgacga atttggcaag ctcaagatcg agggaagtca
    35101 agtgcacata gcgtgcattg ttgaggtagt ccacatcgac atcctcaata gaggggacaa
    35161 gcagtggggt ttcaacaaca accaacgctt tctcccccgt tgagtccaac tggataacgc
    35221 cgaaatatgt aggctcacct tccttgacta ctagtccgcc ctgatccaca ccaaattccg
    35281 ccagaccgcg gatggcaata cggccaaagt cgtcgtcacc aactgttgtg actatcccgg
    35341 tcttggctcc aaacctagaa gccgcacagc agaaattggc gattatgccg ccgggatatt
    35401 tgcccagcaa ttcgcccctt accttttcat catgcccagg caagtggtct accttgataa
    35461 tcaaatcgac acttgcatcg ccgacaccaa tgatatccaa cattttgtgc ccctcttgac
    35521 aagtatttgt agttgcgctt catttgcacc ttcgtgcggt tctagttctt ggcgcacatt
    35581 tgtgcccata ggcgcacatt tgattcttgc tagattgttt cgccacgaac cgcaatactc
    35641 cttctccgtt tcaaatagat tctttgtttg ttgtacaaga aagcggagct ttttgtggcg
    35701 aaaatccttg ggcaacgttg aatttaggac catgcccggg gcttacggac agttttggaa
    35761 gaggttgaat atgatttgcg gggggtatcg aggtggctga ctgagcaagc agaatgagtg
    35821 ttgacaagcg tgcgttatgt aaaacgtgct tctgcgctat tcaagaatcg tagtcttctg
    35881 tcccgtgcaa ttatgtttat attatatgcc tctagcttcg aaaagtatct gagcattctt
    35941 aagcggccaa agtcttaatc tcagacaaaa gcactcatca gcgcatctat caaaccatag
    36001 agcctgttag ctgtggctgc agccttcaaa agccgccatc gcgacactga taccacgtgc
    36061 ttcgtatctc gcaatctact gccgaacaat gttctcatag gatgaacatt cgggctgtct
    36121 tggcgataca gtagccttgg ctcgaaagca gaacctccgg agttgtcggc ggtgagcagc
    36181 tcccatcatt ggcttgtggc gatttcaagg ccaggaggct ataagccttt ctcgccggtt
    36241 acagatcggg ttgtcttcaa tgtatattac ctgctcaagg cgacctagat tcgtgatccc
    36301 aggatcgatc agctctgcgc cgagcctctg ctcggtcgag gcacggcgta cgctgtggcg
    36361 cctgcagctg cttgagcatt catggcagct aggaactgaa atgccaatcc tcatccacag
    36421 catgagtgta atctcataac accgttgtca ggctatgtgc gcaagttcat cgcccctcac
    36481 gcttgtagct tgcggtagat ctcatatgtc cacctagcca gtgcggtgat ctccatcgac
    36541 tcaaatcccg gcaagtaagt ctcgagctgg ttgttgaagg gttccgtcca tcgcggatct
    36601 atgcaatcgg gattcattgc ccccatggcg cagcatacct cacccgcatt gcagtcaacg
    36661 tctgccccgc agtccgccaa gatacgaagg gccttatcga agtcattgcg acagtaccaa
    36721 agcgaatgca cgactgcgac catgttggga taggcatgga tccaattata agtcttgagc
    36781 ttctcttcaa acctcttgta cgattcgata tggtctttat tcgcctgaac agactccatg
    36841 gcgtcatcta gcaccgaagc aaactctgtc ccatcaggca cgtaatctcg ggcgtgcttg
    36901 agaagcactc ttgtgtcatc cacaacaaag gccaatgacg tcaatactgc gctgtgaatc
    36961 cctgcatata ccccattctt ctcatgcgac acaacactgt cgaggtatgc ataccgggca
    37021 gcgcgcaacg gatcccccgg cgccacgaga ccgcatacca tcgtgcgcat ctgagcgcct
    37081 atccactcgg agtagtaatt ccgaaaccta cccgattccg gggggtatat tcctctcctc
    37141 aagttctcca acgcaaagta ctccgccgac catccggacg ggataagctc gagccacttg
    37201 tttgctatgt cgcgggacga gaggcctggc cctgcctcat ccgctgcggc gaggaatgca
    37261 agctcataag tgatgtcgtc attgtaggtt tcaggttctg caacgtaata gttcagcctg
    37321 tcgccatagg cttctctgat cgcatcgcca gtgtagcctt ccatggccgt tccgtagctt
    37381 ccgccgacta tctgccccgt ccagccgccc aggatccgcc tctcaacgtc cgaatcatcc
    37441 acatcttccg catggtcttc gagactttcc ttcatcgcag gcatgcacgc cattatttcc
    37501 tcccaagttt cgggctgatt gtagctccag taaggatggg taggatcctt gggtgcattt
    37561 tcaagagcgt gcatgagctc tgccgtgatt cgctcgagtt ttccgtggtc gcccccggcg
    37621 catactttca aaccgcgttc ggcaattctt tcagcttctg atacatcgta gcccctgtta
    37681 tgccacgctt gaaccgccgc atgcaacagc gcttcagggg ctctcgagcc gggaacttcc
    37741 gattcccaca ataacgcaag catgcctttt ctgtattcga aagcctcgaa gctagcagac
    37801 cagtcccttg ggttctccgt gaaatctact ggcttcgctc tcctcctcat ttcgagctga
    37861 tactcccatg ccttcatagc aagccctccc tatcggacta ctcgtagccc agtccctgca
    37921 aggcccaaac ctcattttgg gccccgcatc tgtattcctc aaattcatct tatactcctc
    37981 tgcccacgtc ggcgcactcg gaatattcac ggaactttca caataccaga aaaggccccg
    38041 gcttgatgct ccgctcctta ttgcatatgc cctatgcttg cagtacacca tagccatgga
    38101 ccaagcatgg atccacccgt cctcaggatg aattcgcggc cctgttcgaa agctcgtgcc
    38161 gttggtatca cttgtcatat cgatactgcc ttgttgagta tctgcttttt cgggggagga
    38221 atggctcgtg gcaaagaaag atagcatcga tgatccaaac ctccgcagcc gcgctcggcc
    38281 tggcttccgc tctctatctc gacctcgacg caatccggtt ggcgttggtc caaacaagct
    38341 caaacgaaat cgaattgctt acaaccggag tcctatacgc tgccgaaggc gatgacgaaa
    38401 cagcctccct tgcgctgact cgcgcccgct ctatggtccc cgatgagcct gaacttctcg
    38461 cccagatatg catcgggttc gcaatcctcg acaggttgga caaggccctc aaaactgcaa
    38521 agatggcagt ggcggcagac gatgggaatg cagccgccca ctacgcattg ggcatgagcc
    38581 tcttcggcat ggaagattat ccggacgctc tgagcaactt cgaaagagct gtcagtcttc
    38641 gaccagacta ctaccttgca catgcttata atgggaaatt cctcgccagg atggaaaagt
    38701 ggcctgagag caggcccatt ccattcgtaa agacctccct agggcaaatg tggaccgcgc
    38761 aatcgctctg ttccgactcg gggccacaga cctcgccgct accagtatag atatgagcct
    38821 ggatgatgcc tacgatatcg gctgttcgca tgttgaccct tgccttgatt aatgatccag
    38881 acaaccacga ctgcgcaaat gaccttattg tttagtgcag actagaacta cggggaaatg
    38941 cagaatggat ttccctgaag tt
//
LOCUS       DAOSYL010000015        37927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_873_length_37927_cov_7.850602, whole genome shotgun sequence.
ACCESSION   DAOSYL010000015 DAOSYL010000000
VERSION     DAOSYL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 37927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_37927_cov_7.850602"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1943
                     /locus_tag="PLU15_04180"
     CDS             270..1943
                     /locus_tag="PLU15_04180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOO30150.1"
                     /translation="MTKIKTDPVRLIPLGGIGEIGKNMLVVEYGDDMIVIDCGLMFPD
                     DDMFGVDLVIPDVSYLEDKKERIRAVILTHGHEDHIGALPYVLKRIGNVPVYGTRLTL
                     ALVQAKLREHGIQLGGNARVISAGDQESFGEISVEFIHVSHSIADCVALAINTPAGII
                     VHTSDFKFDQTPIDGRVMDLRRFAELGERGVLVMLSDSTNADRPGYTLSEKVVGELFR
                     DAFRDAEGRIFVTTFASHIERIQQALDAAAEVGRRVAIVGRSMENNVKLALDLGYLRV
                     KPGTIVDLDTLESLPPSRAVIMTTGSQGEPMSALTRMAMSDHRKVSIVPGDTVIISAS
                     TIPGNERMIGTTINRLFKQGAEVIHEAFSGFHVSGHASQEELKLMLNIVRPRYFVPVH
                     GEYRHLVKHARLAQEVGVPRSDILIPEIGDILEFSRERAEVAGRVASGHLLVDGLSVG
                     AVGNVVLRDRKLLSEDGIVVAIVAADRSRALIVGGPEIVSRGFVYLKESEDFIDDIRS
                     VASDVVEKFEGEGVLEVQSLKDRLRHAISSFIFERTGRRPMVIPVVMDV"
     gene            2052..2792
                     /locus_tag="PLU15_04185"
     CDS             2052..2792
                     /locus_tag="PLU15_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOO30151.1"
                     /translation="MRAESEVEPMQQTPEQEELAGAPLREFGEIETPVSPPSNIHVLS
                     IIGQIEGHTVLPPQNKTTKYEHIMPQLVAIEQSDEIEGLLILLNTVGGDVEAGLAISE
                     LLAGMTTPSVSIVLGGGHSIAVPIAVSCSYSMIASTATMTIHPLRLSGQLVVGTQQTY
                     EYLERMQERVIKFVSEHSRCSTEGFRDLMFRTGELLRDVGTVLVGQDAVDAGLIDSVG
                     SLADALRKLDELIAIRRKIAGRAKERIH"
     gene            2792..3055
                     /locus_tag="PLU15_04190"
     CDS             2792..3055
                     /locus_tag="PLU15_04190"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HOO30152.1"
                     /translation="MLYTVMPIEDVLGEPAWQMDPYAAWRASMGPVEPPMLVSCRVRG
                     VPLSVWPMPDGRGQVERVMSTDPADYLDPTISPGSMVDIREYL"
     gene            3133..4155
                     /locus_tag="PLU15_04195"
     CDS             3133..4155
                     /locus_tag="PLU15_04195"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOO30153.1"
                     /translation="MIMAIPFIGGLALFLLSLVGMSKALGEILGPRTRAALHGASVPA
                     AFVSGVGVTALIQSSTLVSVTLVGLVNSGAMGLAPAFAAILGANVGTTVTAQIAGLRP
                     TWLGWAAAGAGLVLTAWGWVELRGPGAADRVRKETGRMRMLGGAAAVSFSGVILGLEL
                     VAEASGAVMDSDTLKVILTWASHKPILAAVVGAAATAAVHSSSVVSALLVSLTREGLV
                     ELEGAVALVIGSNVGTCFTALVAALPADRDSQTLAWANLIFNVLGAAAFLPFIELFCD
                     LLRLLSTDPGAQLANAHALFNIITAAIVLPFSRQFVELIGGRGKERVYIERHPRVVAG
                     NSPRSH"
     gene            4106..4933
                     /locus_tag="PLU15_04200"
     CDS             4106..4933
                     /locus_tag="PLU15_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOO30154.1"
                     /translation="MSVIQGLLLGIVQGLTEFLPVSSSGHLVVAQALMGLEPNMTVAL
                     GVHAGTALSVLVLYSGEVWEIIMGFFRGVAKRDGSAKMAICLILTSIPAALVGFAASD
                     AVEHAFGSPLFAGIGFLISGVVLWCADCISSRQKQRKQESIDEGNIGYKQALGVGVAQ
                     ACAIFPGVSRSGLTISSALALKFERGFATSYSFIASLPVIVGSLILWPVANPGALAEV
                     QLGTLLLAVAASFVSGLAAMIFLRRVVVRGKLTYFSYYLWAIGIITILLHARGLLGL"
     gene            4966..7608
                     /locus_tag="PLU15_04205"
     CDS             4966..7608
                     /locus_tag="PLU15_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOO30155.1"
                     /translation="MAARQGSQPTRTKKRSKPVRAKPKHAKKSAASQDYALGGELGSE
                     IGGIFLVAGAALGFISLIQGPSGYVGGALANLMISAFGRGAYAVPALMALTGILMLWR
                     RTGHVVFVRFVGLVIVCIAVVIGFELNDFGETALRLPFSQYVADSRSDVGGGLVGAAG
                     AWVFLRLFGFVGSVIISIALGIAGLSIASDVPVATAVTFGAKSIWTGLSFLISMIRES
                     YDAIVLQLFEVAPPYPEDESAVDRADLAVQFREGDCLEADRRAKTAPEPEIGAHSQER
                     GLDRLKEFFQTSEPDAGIVERMEPQEDLPEPSRGESSVGANEVGVAPSKTRPRQSAQG
                     TKSQSIGSLHDEMEQLELINYGLASDLPPTSILKALPKRRARKEVEDPHAMAELLEST
                     LASFGVEASVVGISAGPVVTRFEVQPGPGVKVSKIVNLADDLSLAFASAGVRIEAPIP
                     GKAAVGIEVPSKNRSVIYLREVIESDEFQKAESRLTFAVGKDIAGKPIVADMADLLHL
                     LVAGATGSGKSVCLNSIICSLLFKATPDEVRFLLIDPKRVELSMYDGIPHLLAPVVTD
                     AKKASGYLRWVVEEMENRYRLFQIAGARNIGQYNEMVRLGIADEYIDADANIAGSDVG
                     TDRNIEGSEADAQGGSDVSTEANKGKAAGPRLKPLSYIVVIIDELADLMLVAQREVED
                     AIARLAFMARAAGIHLIIATQRPSVDIVTGIIKSNIPSRIAFAVSSQVDSRIILDSSG
                     AEHLLGKGDMLFHPVGLPKPIRVQGAYISDEEVASLVRYLKQQGKPIYEAKAVETTAS
                     VEEGIAEDDELFPEAVRLVVSSGEASISKIQRRFRVGYARAARLIDTMEVLGIVGPYE
                     GSKPRKVLITEEDLEDALGNRGLV"
     gene            7629..8066
                     /locus_tag="PLU15_04210"
     CDS             7629..8066
                     /locus_tag="PLU15_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO30156.1"
                     /translation="MNEIGVLLREAREASGMSLEEVSEATKIRSKYLEAIEQGDFAVI
                     PGEVYLKGFMRNYADCVGLDGGQVVARYNELSLLKETAALKQLEEERLERLQKSKEEA
                     RSRRAKTILRGLVTAAIGVAAVAGIYYAARAFGFVTWKPTWLP"
     gene            8086..10266
                     /locus_tag="PLU15_04215"
     CDS             8086..10266
                     /locus_tag="PLU15_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30157.1"
                     /translation="MRSVQELLADLARLEGGRFDETLFELVRRGPKALDRLFAYIDSD
                     APPSISAKVNESIGRQWTRVQEVLIELPPKAFSSLGVKALIERGPSDEIARRAAKCIE
                     RGMIETAACIATVLSSSENACIERAILEARLLMAAGRPKRAMARLSPYCGPGEAGTMI
                     SEWMVAAAMAAGEPRRAFLAMPVREPDVTQASHAVGDMIDACSVEDLAESIFDVARRG
                     DLAGWELIGAATEEAWERAVQDDADVALGLAGARSEIEGFLDGIESVLWDLESCPDDV
                     SRGLIQKELSSLVARANEVVRTIGEAYHLAERPAGGCGATGAPGECGGSSWMLTDGDC
                     WDQMLASAKESVGPQIMESLGKSASRQRLPEEINRAVMLAHAEGRPISTRWAEGEFFI
                     EVGLPMTEKSPEACEGSLSRLSSTCAYGGSQIESSLDAGSIARMLRSSQPRQSAWELI
                     WVIRDRERAHATALLGLVAQILEDISHGLNNLSGHSPAAEGAQLEKIRQRLERWDDEV
                     EGCVAKALRNVEDKPEQSEDGGLFGPDRPQDLCFSPESAQALAAADYLLRSARPSVPA
                     PEQAQLISFLIRKAVQTEAECRFDEALADYRLRPAVLRASEARGKRRRKISPAFAERA
                     VKLAPECIPPDRVFGGLDRLASKVIDGSPKPIAGDMFLMALAFMCFDPLGDPDRCYAL
                     GSVLARLDAACQDAERRPDVCDWPGLGRLVCDAISLMSAAGDEL"
     gene            10263..11168
                     /locus_tag="PLU15_04220"
     CDS             10263..11168
                     /locus_tag="PLU15_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO30158.1"
                     /translation="MIEPAHVKGVDYLLTEAANIDIDSLVDLFNDAYSQYYVSTVVTP
                     ERLIKLVDREDIYMHHSFVALYGSRPVGVVFLGVRGERGYICGMGVRIAYQGRGVGEL
                     LMRRVLKEAETLGLNSLQLEVVDRNFRAIGLYTKLGFVPIRDLGIWERNQMPRRKGAG
                     RWNPKGFTLNHVASFQALPLVMEFNEIRPCWQNEPTGLAKFCSTLSALVARADSEKIA
                     YALYVDTVEGLYIVDFAATPSLDEGTRQKACEVLLEAIDKQMPRPTARAHNIPVHGYQ
                     GRVLRGRGFDIGLKQQEMVLWLKSR"
     gene            11150..11419
                     /locus_tag="PLU15_04225"
     CDS             11150..11419
                     /locus_tag="PLU15_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOO30159.1"
                     /translation="MVKVALTTFVTVFLAELGDKTQLATMMLVAEGKSPWAVFLGSAS
                     ALVVSSLVGVLAGGFIGKYVPAMWVRIGAGSAFIILGVLLLMGRF"
     gene            11458..12243
                     /locus_tag="PLU15_04230"
     CDS             11458..12243
                     /locus_tag="PLU15_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO30160.1"
                     /translation="MKDQNRIHRLVLVVCYLAIVCMAMVGLSGADATLARGAIEVADQ
                     LYSRRSEPGNADAIIAGLEQANKATPGDCEILWRLSRAYWFKSTRLPEKDKKARLDLL
                     AQGRAYAQEATEANPRNANAYYWLAASIAQEGQIRGILQSLASVKPTKDALDKGLDID
                     PKHASCHYLMAELYLQLPGPPLSIGNKQKAVEEAKLAVKYDPSESCHHLVLGRALIAT
                     KQYDEARAVLNRLIAMAPDPGDPEGFRKDQDAARAELKSIAGK"
     gene            12298..13614
                     /gene="rimO"
                     /locus_tag="PLU15_04235"
     CDS             12298..13614
                     /gene="rimO"
                     /locus_tag="PLU15_04235"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOO30161.1"
                     /translation="MAFCSLGCSKNLVDTEIMMGLLRDAGMEIVSDEHLADVILVNTC
                     SFIADAAQESVDAILDYADLRADGKLQALLVTGCLPQRYGVELMREIPEIDGIIGTGQ
                     FGRCVEAVEDAVAGRRPAFLEDPGYLQPADAPRVVTTPAHYAYLKIGEGCANRCAYCI
                     IPSIRGGVQSRPLEAVVGEAEKLVAYGMREAIVIAQDTTVYGVDLYGVPSLAKLLRKL
                     ARVEGLEWIRVMYAYPSRIDDELVEVMASTPNIVKYIDLPMQHGSDRLLRAMNRHASR
                     DQMIEVVARLREQVPGIVIRTSLIVGFPGETDRDFEELLDFIEIIRPERAGVFKFSRE
                     EGTRAYDMPEQVPDDVAEARRDAAMEALRRISREYGLSRVGSKVRVIVDGPSDESDLV
                     VEARSFAEAPDVDGKIYIGDATLRPGQLVDVIIEDASDYDLAAVLV"
     gene            14188..15249
                     /locus_tag="PLU15_04240"
     CDS             14188..15249
                     /locus_tag="PLU15_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOO30162.1"
                     /translation="MNAQVAEVVDVVSEFPDLQELSVKLAENGCTVRAICYPKITGTA
                     QPGDEVIVNTTAVDLGLGSGGTHFVVWVLGRDRLSNQLPGHIMKMRYTPMQLATGSYE
                     ENPAYRGAIDSFSGLDGMPVVICELHSMVAPVLAGFSGKAAYIMTDGAALPYALSKVV
                     RRLKEERLFHVSITAGHAFGGDLEAVNVHSALVAAYAVCRCEGAVVAMGPGIVGTGTQ
                     YGFSGIEQAHIADAVNRMGGRAIIVPRLSRCDPRERHHVLSHHTVTVLADVCCTPATC
                     VLASDMDDGFRRKVEERLCGILKAGGHRLAYAPGGGAIDELKERGIELSTMGRGFDEE
                     PEFFLTSAAAGAYARKLVH"
     gene            15581..16162
                     /locus_tag="PLU15_04245"
     CDS             15581..16162
                     /locus_tag="PLU15_04245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO30163.1"
                     /translation="MKQDLTEKAISSETIYNGKIVTLNVDKVELPNGRTATREVVRTR
                     DSVAVVPVTRDDKVVLVRQYRYAGGEELLEIPAGRIDPGESPRAAAQRELLEETGCTC
                     AELKKIAGFYIAVGFATEFMHLYAGTVDHIGEACPDDDEFVRTELMPVSQIPEILAKQ
                     EIRDVKTLAGLLHLSWMLGLSPVSSCACSICRA"
     gene            16226..17428
                     /locus_tag="PLU15_04250"
     CDS             16226..17428
                     /locus_tag="PLU15_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOO30164.1"
                     /translation="MSERGLNRYFVDLHVHIGRDSAGKPVKVSGARNLTFANIAKEAA
                     TRKGIDIVAVVDCASTRVLKDIEALLESGDMTEAPGGGFRYLDKTTVIPGAEVESACP
                     GSARGAHYLSLFPTLTALRQYSRSLSRYVSNMDLSTQRARLSPVELAQLAADSGGLFL
                     VAHAFTPHKSLYGTCARRMSEVFGEDVRKLVDGVELGLSADTDMADRIGELSHYTFTS
                     NSDAHSLPKIGREYNVMRMKAPSFSEVRLALARLDGRRVVANYGLDPRLGKYHLTACE
                     ECGHRFKAWPNSCACPVCGSVRTVRGVADRIDELSDTAEPIHPPHRPSYNYQIPLEFV
                     PGLGPARIGALIDAFGTEMTVIHETIESDIAEVVGEEIAHRICLAREGRLGIQSGGGG
                     AYGKAVVE"
     gene            17492..18127
                     /gene="spoIIM"
                     /locus_tag="PLU15_04255"
     CDS             17492..18127
                     /gene="spoIIM"
                     /locus_tag="PLU15_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOO30165.1"
                     /translation="MARARRTSRSVLSHARSNMVGYWLTFVVFITGVSCGAVQASMLK
                     EGRTELFSYLEAGLASLRHTLPVAPGKALTYSLGANLASVGLIWLSGLTILGFPGALV
                     ILFVRGFSIGFTVAFMVRELSGPGAALAVASVLPHNILAVPGLILACFVSMSFSWAVI
                     GTNLIGMPSDVKLAFSRSVAGVAISAILLSAASFVQAYVSPAILILAARFV"
     gene            18707..19633
                     /gene="xerD"
                     /locus_tag="PLU15_04260"
     CDS             18707..19633
                     /gene="xerD"
                     /locus_tag="PLU15_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOO30166.1"
                     /translation="MLPSVEEYINYLAVERGLSENTLEAYARDLSQFAEFAAKSGRQS
                     DEPIDDDILTASQSMVASFIARSIESGGSPSSAARRLTSLRRYYRFMLQEGYVKADPT
                     ANLESPRQPQRLPDVLTEEEVNRLLSSAPGYDPQSLRDTAMLELMYASGLRVSELISL
                     TTEDVDMELGYVRCVGKGDRERIVPVGRRAIEAIERYLAFGRRELAGVCKDNYLFITN
                     RGTAMTRQGFWKMIKRRALKVGISTDITPHTLRHSFATHMLENGADLRSVQELLGHAD
                     IRTTQVYTHLTQVRLREVYNKHHPRALGEGDA"
     gene            19630..20826
                     /locus_tag="PLU15_04265"
     CDS             19630..20826
                     /locus_tag="PLU15_04265"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOO30167.1"
                     /translation="MSMRALIIVFDGVGIGELPDAGEYGDLGSNTLVNTSRAVGGLNL
                     PNLEKLGLGNLAAAAGHEICGVAAIEHPLASYGTMAEKSPGKDTTTGHWELVGIQLDQ
                     AFPTYPKGFPPEIILPFQEAIGRKVIGNIPASGTKIIEELGEEHIRTGSPIVYTSADS
                     VFQIAAHEEVIPPAELYEMCKIARELLSGPHAVGRVIARPFIGVPGNFIRTERRRDFS
                     LVPPGPTLLDLVAREGLEVMACGKIDDIFANQGITRSKHVAGNDAIFDAVLEFLDSGH
                     SGLIFANLIDFDMKWGHRNDVEGFARGLEHIDSRLPELLERLGESDLLAICADHGNDP
                     TTPSTDHSREYVPVLAYSPARQRKGRCGAHVGVRETFSDLGATVADFLEVGWTLRGES
                     FLNEIL"
     gene            20856..22163
                     /locus_tag="PLU15_04270"
     CDS             20856..22163
                     /locus_tag="PLU15_04270"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOO30168.1"
                     /translation="MRVPDIIMKKRDGGELSPEEIFFLIDGMVSGDVPDYQMSAFCMA
                     VYFQGMTSAETTALTLAMARSGEIVDLSAIRGTKVDKHSTGGVGDTTTLVLAPMVAAT
                     GVPVAKMSGRGLGHTGGTLDKLESIPGMRVSLSEEEFIEQVNRIGIAIVGQTEDVAPA
                     DKILYALRDVTATVESIPLIAASIMSKKLATGADAIVLDVKTGTGAFMHDLSQAEALA
                     GLMVDIGRMARRKMAAVISDMNQPLGHGVGNTLEVIEAVQTLAGEERGRLFDLCLELG
                     ARMVVMGGAESSIEAARVRLNDVVDSGAALEKLREMVVAQGGDPAYIDDPRRLPVAPY
                     SEYITAPCSGYVASVDAMTLGRAAMSMGAGRARKEDSVDLECGLVTLVSIGDPVEQGQ
                     PVAKLYASSTSLLTEGLSVAAKAYTYSAEPIEPPPLVYRIIES"
     gene            22238..23452
                     /locus_tag="PLU15_04275"
     CDS             22238..23452
                     /locus_tag="PLU15_04275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOO30169.1"
                     /translation="MSYIRCGRACRAALALVALLLVVKVGICGAQSAPLAAMSLDLDK
                     KITAPSAILMEASSGRVLWEKNADVARPIASVVKVMTLTLVMEALEAGRIDLDDIYIT
                     SEHAASMGGSQIWLEPGESMAVSELVKAVAIESANDAAVALAEHVAGSEPAFVALMNE
                     KAQALGCKNTHFINATGLPGKSPSEDCVSSARDVAIMSRELLKHPRIHEWLTIWIGYV
                     RDGQNMLTNTNRLIRFYPGADGVKTGYTERSRYCLSATAVRNNVRVIGVVLGVPTSAV
                     RFSEASTLLDYGFKMCEAHVIARSGQEMGRVRVLRGIDTEVGVTVDSDFTVVTLRGEG
                     DGVDARLELPQYVSAPVERGQRVGKIVAYVEGVEINSADLVAVDDIPKAGAFTLVVRS
                     TKEFVRSLFDFR"
     gene            23547..23909
                     /locus_tag="PLU15_04280"
     CDS             23547..23909
                     /locus_tag="PLU15_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOO30170.1"
                     /translation="MIEWEVSGNSLVVEIAGELDLTCADEFRRVLDSLIDSHPGVARV
                     ILCMDRVAFIDSSGIGVLFGRHRRMALRGGTLAIASPSPQAMRALDLLGVPSVIHVYD
                     TLKQALAAEAEVRKCTRK"
     gene            23894..24331
                     /gene="spoIIAB"
                     /locus_tag="PLU15_04285"
     CDS             23894..24331
                     /gene="spoIIAB"
                     /locus_tag="PLU15_04285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOO30171.1"
                     /translation="MHAKVNHAKVEFDAIPGNLGFARVAAASFAAGLGFTVEDLEDIK
                     LAVSEAVSNCVIHAYPGPGEARPTVLMVMEARESEFVVSIEDKGIGIADVAMARKPGV
                     TTDSERLGMGFTLMEALADQVDVKSELGVGTRVEMIFSPERGG"
     gene            24328..25038
                     /locus_tag="PLU15_04290"
     CDS             24328..25038
                     /locus_tag="PLU15_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO30172.1"
                     /translation="MMWNADTQGESAFLSSREAEARRHAGLVMSLARRFAGRAEIADL
                     YQAGCVGLLRAIDRYDQSRGTTFSTFAVPIILSEIVAYLRGSGPIHVPRPVRELAQKC
                     RHTQDSLRAELSREPQATEIAARLGIDAADVVYAMESEAHVYSLFDQVSFDHESLELH
                     EVLPGDLGVETSVCESIALSDALSALSEIERTVVAMRFFEERTQAETASLLGISQPQV
                     HRIEKAALLALRRSMEGE"
     gene            25084..25551
                     /gene="spoVAC"
                     /locus_tag="PLU15_04295"
     CDS             25084..25551
                     /gene="spoVAC"
                     /locus_tag="PLU15_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOO30173.1"
                     /translation="MASPGAKKQKADERAKYQRLAASIVPKPPLVRNMVVAFVVGGLI
                     CTVGQGVINAFMSRGMTQDEATAPTLATMILIGSVLTGLSAYHHIGEFAGAGAAIPIT
                     GFANTIVAAAMDFKREGYILGMGAKMFIIAGPVLVYGILSGVLVALIRWLYRG"
     gene            25555..26562
                     /gene="spoVAD"
                     /locus_tag="PLU15_04300"
     CDS             25555..26562
                     /gene="spoVAD"
                     /locus_tag="PLU15_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOO30174.1"
                     /translation="MTTKRVGGSTIVYSTPPVIVSAATVAGPKESEGQMAQWFDELVP
                     DDMFGERTPEKAERRFMEDAIKIALGSARVLPEQVNMIFAGDLLNQIITSSFTAEGFG
                     IPFVGLYGACSTMAQSLTMASAMVDGGYADLALAVTSSHYQTAERQFRYPIELNIMRK
                     STSQYTVTGAGAALVGRSGGKVKVTEATIGKVIDMGIKDPNQMGPAMAPAAADTIFAH
                     LADTGRDPGYYDIILTGDLGRVGAEILCQLMAEKGVPLGDNYSDAGILMFEGVRGTGV
                     GGSGCACSALITYGFILKRMCQNEISRALVVATGALMSPISWQQGESIPCIANAVVLE
                     T"
     gene            26623..26973
                     /gene="spoVAE"
                     /locus_tag="PLU15_04305"
     CDS             26623..26973
                     /gene="spoVAE"
                     /locus_tag="PLU15_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOO30175.1"
                     /translation="MDYLVAFLVGGTICAIGQVLLDTSKLSPPHMLVAFVVAGAILSG
                     LGVYDKLVDFAGAGALIPVSGFGHSIARGSLMEAERLGVLGLFTGALEFTGLGITTAV
                     FFGALIALVANPKP"
     gene            26976..27965
                     /locus_tag="PLU15_04310"
     CDS             26976..27965
                     /locus_tag="PLU15_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOO30176.1"
                     /translation="MRVGVPRGLLYHEYAPAIHTFLESCGAETVVSPQTNREILEKGL
                     ALCVDDACLPVKAFFGHAAVLSDQVDALFVARFVSVERGAYICPKFMGLPDMIRARLQ
                     NAPPIIDMCVDIRQGLGSYVSSLPCSDTCKVLGVAPRHLSWALGAALMAQRKEDRRRV
                     KRFAPSGSAVARTGLGGYTRESIYETSLTIGVLGHAYNLDDAYLSLGLISKLEKMGVK
                     AVTAEDYAYDNLRRWERRGADRKQVFWTAGRKLIGAARRWRETGEVDGMVHLTSFGCG
                     PESFVAEVIARDAARHPAMPLIILNVDEHSGEAGMATRLEAFVDTVRMGKSKR"
     gene            27962..29170
                     /locus_tag="PLU15_04315"
     CDS             27962..29170
                     /locus_tag="PLU15_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOO30177.1"
                     /translation="MKVSFPHLGNLWIPLKAFFSGIGIEVVAPPPTTRKTVAIGAQLA
                     PETACLPLKVTIGNMIEAINSGADTFLMVGGIGPCRLGYYCELQRDILSDLGDDVDLV
                     VIEPPRGHFRNTVRFARLLTSRAGVRDVVEAGKLAWEKCIGIDVCEQLANAARWGEAE
                     RGAVDRAVRKGLAELDRASTIRDVQAVIANTVEHVRSCAVVGPICQLDSCARSLVTDR
                     YGALLRSEEGDISPLSVGVVGEIYVVMEPAVNMNLCKKLGTIGAQVMQPITFSGWIMT
                     HVVLDIVKFMPKPDMLAWATPYLGHFVGGHGVESVGHSVQMARSGFDGVVHISPLTCM
                     PEIVAQSILPRVSEEEGMPIMSLTIDEHTADAGLDTRLEAFCDLLAERSGKRLDGISG
                     AGRWKDMQVL"
     assembly_gap    29268..29367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29475..30086
                     /locus_tag="PLU15_04320"
     CDS             29475..30086
                     /locus_tag="PLU15_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO30178.1"
                     /translation="MNPYSMLITLPILLFSLSVHEYAHARVAYRLGDNTPRWQGRLTL
                     NPLAHMDPVGLLVLVVTGRFGWAKPVVVNPNNFENRDAGMVMVSLAGPGSNLVLAIAL
                     ATLFKAVSGPYGIPANAFARYLALALNQGVWINLGLAVFNLLPVPPLDGSKILAALLK
                     GKGARVYYTLEKYSYIVLLLFIATPLASWILSPIILTLYRAIM"
     gene            30099..31079
                     /gene="trpS"
                     /locus_tag="PLU15_04325"
     CDS             30099..31079
                     /gene="trpS"
                     /locus_tag="PLU15_04325"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOO30179.1"
                     /translation="MAKGRILSGNRPTGKLHLGNFAGALANWVKLQDDYECFFMVADW
                     HALTTGYEDTSELKNYTRQMLIDWLSAGLDPERSAIFRQSDVKEHAELHLLLSMITPL
                     GWLERCPTYKEQLQQLSGKSITNYGFLGYPVLMAADILVYKADTVPVGEDQLPHLELA
                     REIARRFNYLYTPVFLEPQAKLSQVPILPGIDGRKMSKSYGNTIALSDPPEKVREKVN
                     AMVTDPQRIRKSDPGHPDVCTVYAYYKGLAPELIDGRRQECEAGKIGCVACKKELANV
                     LLEILGPIHERRHELEAQPGFVESVLEEGAAKARPIAVQTMAEVREAMRI"
     gene            31076..31852
                     /locus_tag="PLU15_04330"
     CDS             31076..31852
                     /locus_tag="PLU15_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOO30180.1"
                     /translation="MKVETPVFSGPLDLMLSLIEKKEIDICEVSISEITDDYLEHVSS
                     MEWLDIDQAAEFLVIAATLLYMKVKALLPEQELAEEFGVEEDELPDPGADLVARLIEY
                     RRYRDAARVMRSLEAAASRMYTRPPRPLPKPAMVKVNPLDGISAKDLFDLYVKAATSV
                     PEEFTHLPEDEIDIVPQMASVLTRLARRGRLGFHEFLGEDASRPVIVATLLAMLELVR
                     RGRISATQPMPFGEIWMFPQKEKASSGGVSDESPRSASGN"
     gene            31812..32525
                     /gene="scpB"
                     /locus_tag="PLU15_04335"
     CDS             31812..32525
                     /gene="scpB"
                     /locus_tag="PLU15_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOO30181.1"
                     /translation="MCPTNLQEAQAAIEAILFAASFPLRAEEIASAIGVDESSTRKLL
                     RLIGDRHSSPESGVALVETGGGFIMLSRPEYAPYIERLSERQRPPSLSPASLETLAIV
                     AYRQPVTRGEIERIRGVSPDSAISTLLERGLICEKGRKESIGRPILYGTTPDFLLYLG
                     LSSLDDLPVLPDGEGLPADAPQGADEQDALMSPLPGLGKDGDEAGGNAHWDMPDGIDL
                     ESMFDGMSATDANCDEEDD"
     gene            32630..33229
                     /locus_tag="PLU15_04340"
     CDS             32630..33229
                     /locus_tag="PLU15_04340"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOO30182.1"
                     /translation="MRWVVALLSVLAIVVAALVALIFWPIRLRLSAHSGEMCASVAAR
                     VSLWPHLFSFQVYSHDIIPGTSQPHKTPTQRLASVKETIDRFQALVPVLSAALSGHHK
                     LISVVWHSLVGTGDAALTAIACGSLWAAKSTLLSSAYARWGKRSDLPEYSVTPLFDRQ
                     CFLTDLDISLETSIASVILAPSFSSAVMGFVKAGRKRRE"
     gene            33301..33756
                     /gene="ytfJ"
                     /locus_tag="PLU15_04345"
     CDS             33301..33756
                     /gene="ytfJ"
                     /locus_tag="PLU15_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOO30183.1"
                     /translation="MPEHPIEGLMKTTLESIRQMIDVNTILGDPVETKDGNVIMPVSR
                     VTFGFASGGTQWDASRRRDGGQPPTGSGGGTAELPFGGGAGAGVSLSPVGFLVVGNNQ
                     VRFLPVTTDAIFDRLIDVVPDLIARLAAPQPKTITVRTQAQPEVKRPIA"
     gene            34000..34647
                     /locus_tag="PLU15_04350"
     CDS             34000..34647
                     /locus_tag="PLU15_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOO30184.1"
                     /translation="MQQIAVIGLGRFGTAVARTLSDLGQEVLAIDVDEEKVQKLAPQV
                     TTAVVANVTDIDAMRAVGIRNVDVAVVAVGTELKASIVGTMVMKELGVKSIVAKANDD
                     LHGKVLEKVGADRVIYPERDTGARLARMLTSTNIIEQIDLDPNYSMVEMVAPSHIAGR
                     TLAALNIRARFGVYIMAIRSAGRMIVAPGGDDVIHEGDVVVVIGQNDNLQRFKRL"
     gene            34716..35498
                     /locus_tag="PLU15_04355"
     CDS             34716..35498
                     /locus_tag="PLU15_04355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOO30185.1"
                     /translation="MERVHKFLARAGVASRRKCEELIAEGRVSVNGVTLKQPGVKVDP
                     DRDVVAVDGVIVRPPSEHLYIAVNKPAGFVTTVRDTHGRPTVLDLVPGLDRRVYPVGR
                     LDMESEGLLLLTDDGELAYGITHPSRGVTKTYRVRLAEPILQAQLDVVASGIELEDGW
                     TAPARASMIDNSGRLVEIELREGRKRQIRRMFAAIGNEVVQLVRIAIGCVSLGSLALG
                     ASRELTSEEIRGLRELAGGREQAELDGGGFRPDATGKGKSKV"
     gene            35467..35898
                     /locus_tag="PLU15_04360"
     CDS             35467..35898
                     /locus_tag="PLU15_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30186.1"
                     /translation="MQPVRARVRFDYRGVAVPGRLFIGHKGAAEASAALRDRATANLK
                     YEVEQGIRVEDVDSGHETYVLIDPATDQEVAYSPVYVVVQADSIEDLADFILRDEFRT
                     IEILDPPELTLSPQEIGRLLARANQRLRTMAQLVVRRSERY"
     gene            36018..36410
                     /locus_tag="PLU15_04365"
     CDS             36018..36410
                     /locus_tag="PLU15_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO30187.1"
                     /translation="MELFGSRSGFTLVETMTAIVLLSLVVLIVSAGVQGSLSLSRKGK
                     SKAEGVAILKAVTEDLSTTPATLKDVADKGEKSYPLEAFDFDPSYEARYSVHGTAHGV
                     YRLEVRVIRDGNCIASNEMLIRMPDIGQ"
     gene            36352..36915
                     /locus_tag="PLU15_04370"
     CDS             36352..36915
                     /locus_tag="PLU15_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO30188.1"
                     /translation="MGIASRPTKCLSGCPTSANRRGFWRRPEAGITLIELMIAVSIMG
                     FTLIGTLAFTRLGASTKTIVDRDVLYSQELSLMSDQIIDGTSTRYRGLREAADVTSDN
                     RLGYTRYTFHFHGSDASYQEHYWAVDAVLYRSRGTEPPEQMGSANSIEVSGPDARGMC
                     TLRLGFTDPEGHEVEHITSVRLRNHSR"
     gene            36988..>37927
                     /locus_tag="PLU15_04375"
     CDS             36988..>37927
                     /locus_tag="PLU15_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30189.1"
                     /translation="MVALSPSTDERGSSLVYTLVVVVVLTIAVVSLLQLAAMSFKTGK
                     FEEERQQALAAAESGIAVANARLVSNDPMPTSAYPSSVPSIDSTAAHWIGPGTGPIPK
                     ELLLGDNVSYRVWVSPPVDGRFTVVSEGRCGGRTRVIQQALSSSTTGLPLLMSATSTQ
                     LFGIILNGSGSLDGDLHTEGKDAWSVVANLSTPANGTLYVRPDADIKGIRRQVGTYFR
                     SVEVDPNTYRYPVPVCPEGLAHRGKFRANGWEPIVITKSGEYDEFIVNGSCEMIVDAS
                     ESDIIIHTKEDFLINGSVNLKIIGDHALKLYVDGNFT"
BASE COUNT         8393 a         9388 c        11500 g         8546 t
ORIGIN      
        1 atagaatggc agtgcctctc gagtatttgc attttccgag gccacttatt gtaagtggcc
       61 tcctttgatg acttatgctt ttgcgggtgg gggagaatcg agtcggtcag acaatccagt
      121 aattgatgga tggtgaatac ccagatggcg tggctgcaaa gcatgtgcca aaatgggcgc
      181 gtcgatctgt ctccttgata cgcaatggcg ctaggtatat aattctagca gcttgataca
      241 atgagaatct gcgaggtgca gtataagtag tgactaaaat caaaacagat cccgtgcgct
      301 tgatacctct gggtggaata ggcgaaatag ggaagaacat gttggttgtg gaatacggcg
      361 acgacatgat cgtcatcgat tgcggattga tgtttccgga cgatgacatg ttcggagtcg
      421 atctggtgat ccctgacgtc tcatatctgg aagacaagaa agagcgcata agggcggtaa
      481 ttctgactca cggccatgaa gaccacatag gggcccttcc atacgtactc aaacgtatcg
      541 gcaacgtacc ggtgtacggc acaaggctca ctctggcttt ggtccaggca aaactgaggg
      601 agcacggaat tcagctgggc ggcaatgccc gcgtcataag cgcgggcgac caggagagct
      661 ttggagaaat cagtgtggag ttcatccatg taagccattc cattgccgat tgtgtggctc
      721 ttgccatcaa caccccggct gggataatag ttcatacttc tgacttcaaa ttcgaccaaa
      781 ctccgataga tgggcgggta atggacttgc gccgcttcgc ggaattgggt gagcgcggcg
      841 tcttggtgat gctttccgac tctaccaatg ctgatcgtcc tggatacact ctgtcagaga
      901 aagtagtggg cgagcttttc cgggacgcat tccgtgacgc cgagggaagg atatttgtta
      961 caacttttgc gtcgcatatc gaacgcatac agcaagcgct tgatgccgcg gctgaagtag
     1021 ggcggcgggt agccatcgtg gggcgctcga tggagaataa tgtaaagctt gccttggatc
     1081 tgggttatct tcgcgtcaag cccggcacca tagtcgatct tgacacgttg gaatccctgc
     1141 ccccgagccg cgcggtgata atgaccactg gcagccaggg agaacccatg agtgctctaa
     1201 ctcgaatggc tatgtctgat cacagaaagg tgtcaatcgt accaggcgat acagtaatca
     1261 tatctgccag tactatcccg ggaaacgagc ggatgatcgg aaccactatc aaccgcctct
     1321 tcaaacaggg cgccgaggtg atacacgagg cattttcggg ttttcacgtt tcgggccatg
     1381 caagccaaga ggagctcaag ctgatgctga acattgttag gccccgatac ttcgtccctg
     1441 tgcacggaga atacaggcat cttgttaagc atgcgaggct cgctcaggag gtcggagtgc
     1501 caaggtccga catactcata cctgagatcg gcgatattct cgagttcagc cgggaacgcg
     1561 cggaagttgc gggccgtgtt gcctcagggc atcttctcgt tgacggcctt tcagttggag
     1621 cggtaggcaa cgtggttctg cgagacagga agcttctttc ggaagacgga atcgtagtag
     1681 ccatcgtggc agccgacagg agtcgagcgc tcatcgtggg cggccccgag atcgtgtcgc
     1741 gggggttcgt ttacttgaag gagtctgagg atttcattga tgacataaga tccgtggcct
     1801 ccgacgtagt ggagaagttc gaaggcgagg gcgtgctgga ggttcagtca ttgaaagacc
     1861 gtctgaggca cgcaatatcc tctttcatct tcgaacgcac cggcaggcgt ccaatggtca
     1921 ttccagtggt aatggatgtg tagcctgaat gcgcgggcgg tttcttcccc gccttcgcat
     1981 tccgatacat aattactctg ctcacaccct catactaacg ttgacttcac tgacggaggt
     2041 gcaaacggca tatgagggct gaatcagagg ttgagccgat gcagcagacg ccggagcaag
     2101 aggagctggc aggcgctccg ttgagggaat tcggggagat cgaaaccccc gtttctccgc
     2161 ccagcaacat acatgtattg agcattattg gccagataga gggccatacc gtgctgccgc
     2221 ctcagaataa gacgaccaag tacgagcaca taatgcctca gctcgttgct atagagcaaa
     2281 gcgatgagat tgaggggctt ctcattctcc tcaacacggt gggaggggat gtcgaggccg
     2341 ggctggccat atcggagttg cttgcaggga tgacaactcc gtcagtcagc atagtccttg
     2401 gaggcgggca ttccatcgcg gtcccaattg cagttagctg cagctactcg atgattgcga
     2461 gcactgccac gatgaccata cacccgctgc gtctatcagg ccagcttgtg gtgggaacac
     2521 agcagacgta tgagtacctc gagcggatgc aggagcgggt aataaagttc gtctcggaac
     2581 actccaggtg ttcaactgag ggcttcaggg atctcatgtt caggacgggg gaactccttc
     2641 gggatgtggg cacagtcctg gtaggacagg atgccgtgga tgcagggcta atagactctg
     2701 tgggaagcct cgctgacgcg ctccgaaagc tggacgagct catcgcgatc agacggaaga
     2761 tagctggcag ggcaaaggag cgtatccact gatgctttac acagtcatgc ccattgaaga
     2821 tgttctgggc gagcctgcgt ggcagatgga cccttacgca gcctggcgtg cctccatggg
     2881 ccccgtagag ccgcccatgc ttgtctcgtg ccgcgtgagg ggagtgcccc tctcggtctg
     2941 gcctatgcct gatggcagag gccaggtgga acgggttatg tcgactgatc cggctgacta
     3001 tcttgaccca acaatttcac ccggatccat ggtggatata cgcgaatacc tctagcagcc
     3061 tcgccacata acctctccgg ctgcggcata cggttttctg actggatgct catgtcgtga
     3121 ggtggtggca aaatgattat ggcgatacct tttatagggg gtctcgccct tttcctgctt
     3181 tcacttgttg ggatgagcaa ggccctcgga gagattttgg gcccgcgcac gcgtgcggct
     3241 cttcatggcg cctcggtgcc ggctgctttc gtatcgggcg tgggcgttac agcgctgatt
     3301 cagagctcga cgctggtaag tgtcaccctg gtcggcctcg tcaattcagg cgcgatgggg
     3361 ttggccccgg cttttgctgc cattcttggg gccaacgtcg gaacaaccgt gacggcgcag
     3421 attgcggggc tccgcccgac atggctcgga tgggcggcag ccggggcagg gctcgtgctc
     3481 acggcgtggg gatgggtgga gctccgcggt cccggggcag ccgaccgcgt caggaaagag
     3541 acagggcgca tgcgaatgct gggcggcgcg gcggcagtca gcttctccgg agtcattctt
     3601 ggcctggagt tggttgccga agccagtggt gccgtgatgg actcggatac actaaaggtc
     3661 attttgacct gggcatcgca taagccgatt cttgcggcgg tagtaggcgc cgccgctacg
     3721 gcggcagtcc atagcagttc ggttgtgagc gcgcttttag tctcactgac cagggaaggc
     3781 cttgtcgaac ttgagggggc ggtggctctt gtcattggaa gcaatgttgg cacgtgtttc
     3841 actgctctgg ttgcagcctt gccggcagat cgcgactctc agacactggc gtgggcgaac
     3901 ctgattttca atgttctggg ggcagcagca ttcctcccat ttatcgaact cttctgcgat
     3961 ttacttcgcc ttctttctac cgatcccgga gcgcagttgg cgaatgcgca cgcattgttc
     4021 aacataatca ccgcggcgat cgtcctccca ttctcacggc agttcgtaga gttaatagga
     4081 ggccgcggca aggagcgagt ttacattgag cgtcatccaa gggttgttgc tgggaatagt
     4141 ccaaggtctc actgagtttc ttcctgtatc aagttccggg catctcgtcg tagctcaagc
     4201 gctaatgggt cttgaaccca acatgacggt agcactgggc gttcatgctg ggactgcact
     4261 ttccgttcta gttctgtaca gcggcgaggt atgggagatc attatggggt ttttccgcgg
     4321 cgtagcgaag cgcgacggta gtgccaagat ggccatatgt ttgatcctca ctagtatccc
     4381 ggcggccttg gtcggtttcg ccgcgtctga cgccgtggag cacgcgttcg gttcgcctct
     4441 ctttgctgga atcggattcc ttatctcggg agtcgtccta tggtgcgccg actgcatatc
     4501 gtcgcggcaa aagcagcgga aacaggaaag catagacgag ggcaatatcg gttacaagca
     4561 agctcttggc gtgggcgtcg cacaggcgtg cgcgatcttc cccggggttt cacggtcagg
     4621 cctgaccatc tcgtccgcgc tcgccctcaa attcgaaaga ggatttgcaa cgtcatactc
     4681 gtttatcgcg tctttgccgg tgattgtggg cagccttata ctctggcctg tagcaaaccc
     4741 cggagctttg gccgaagtcc agcttggaac tcttcttttg gctgtagcag cctctttcgt
     4801 atcaggactc gctgccatga tctttctgag acgcgtggtc gtcaggggaa agctgacata
     4861 cttctcatac tacctatggg cgattgggat catcaccatc cttttgcatg ctagaggatt
     4921 gttgggactg taagtagaat tgtgtgctta cggtggtgaa gaggtgtggc agcacgacag
     4981 ggaagccaac ctacccgaac aaagaaacgc tcgaagccag tgcgtgcaaa gcctaagcat
     5041 gcgaagaaat cggccgcttc gcaggactat gccctcggcg gcgagctggg ctcggagatt
     5101 ggcggcatat tcttggtagc aggggccgct cttggcttta tttctcttat tcagggccct
     5161 tcgggttacg taggtggggc actagccaat ctgatgatat cagcgttcgg gcgcggggca
     5221 tacgcagtcc cggccttgat ggcgcttact ggaattctca tgctctggcg tcggaccgga
     5281 catgtcgtct tcgtccggtt tgtagggctc gtgatcgtgt gcattgccgt tgtaataggc
     5341 ttcgagctca acgatttcgg ggagactgct ctcaggcttc ctttttccca atacgtcgcc
     5401 gattcacgtt cagacgtagg aggagggctt gtgggcgctg ccggtgcctg ggtatttctc
     5461 aggctttttg gatttgtggg ttcggtgatt atctcaatag cccttggaat cgcagggctt
     5521 tccattgctt cagatgttcc tgttgcaact gctgttacct ttggcgcgaa atcgatatgg
     5581 acaggcttgt catttctcat aagcatgatc agagaaagct atgacgcaat agtgcttcaa
     5641 ctgttcgagg ttgctccccc gtatccggag gatgaatctg ccgttgatag ggccgacctg
     5701 gctgttcagt tccgagaagg cgattgcctc gaagctgaca ggcgggcaaa gaccgcgccg
     5761 gagccggaga ttggagccca ttctcaagaa cggggtctag atagactcaa ggagttcttc
     5821 cagacatcag agcctgatgc aggcatcgtg gaacgaatgg agcctcaaga ggatctgccc
     5881 gagccttcca gaggtgagtc gagtgtgggt gccaacgagg taggtgtcgc gccttcgaag
     5941 acacggccga ggcaatctgc tcagggcaca aaatcgcagt cgatcgggtc cttgcatgat
     6001 gaaatggagc agctagagct gataaactat ggacttgctt ccgatttgcc tccgacgagc
     6061 attctcaagg cgttgccaaa gcgtcgtgcg cgcaaagaag tggaggatcc gcatgccatg
     6121 gccgaactgc tggagtctac tcttgccagc tttggcgtgg aagcttctgt agtcggaatc
     6181 agcgccgggc cggttgtcac gcgttttgaa gtccaaccag gtcccggtgt caaggtatcg
     6241 aaaatcgtca acctcgctga tgacctgtca ttggcctttg cctcggcagg tgtcagaata
     6301 gaagctccga taccgggcaa ggccgctgtg ggcatcgagg ttccgagcaa aaacaggtct
     6361 gtaatatact tgcgtgaagt aatagagtcg gacgaattcc aaaaggcgga atcccgcctc
     6421 acctttgcag tgggcaagga tattgctggc aagccaatag tggctgatat ggccgatctc
     6481 ctacacctct tggttgcggg ggccactggt tctggcaaga gcgtatgcct gaattcgatc
     6541 atatgcagcc tgctgtttaa agcaaccccg gatgaggtca ggtttcttct cattgatccc
     6601 aagagagtgg agctttctat gtacgacggg attccgcatc tcttagctcc ggtggtgaca
     6661 gatgcgaaga aagcttcggg ctacctcaga tgggtggtgg aggaaatgga gaacaggtac
     6721 cgcctcttcc aaatagccgg agcgcggaat attggccagt acaacgagat ggtacgtctg
     6781 ggaattgcgg acgagtatat cgatgcagac gccaatatcg ctggatctga cgtcggcaca
     6841 gacagaaata tcgagggatc agaggcggac gcgcaaggcg gttcagatgt ctccactgaa
     6901 gccaacaaag gaaaggctgc ggggccacgg ctgaagcctc tctcgtacat cgtcgtgatc
     6961 atagacgagc ttgctgattt gatgctcgtt gcccagcgtg aagtggaaga cgctattgct
     7021 cgattggctt tcatggcgcg ggctgccgga attcatctga ttattgcgac gcagaggcct
     7081 tcagttgaca ttgtgaccgg aattataaag tcgaacatac cgtcgagaat agcgtttgcc
     7141 gtttcctcgc aagtggactc caggatcatc ttagactctt caggcgcgga gcatctgttg
     7201 ggaaaaggtg atatgctgtt tcatcctgtg ggactgccaa agcccatacg ggtgcaggga
     7261 gcctacattt ccgacgaaga ggtggcaagc ctggtcagat acttgaaaca acagggaaag
     7321 cctatttatg aggccaaagc tgtggaaacc accgccagcg tggaggaagg catcgctgaa
     7381 gacgacgagc ttttccccga agccgtcagg ctcgtcgtca gttcaggaga ggcgtctata
     7441 tcgaagatac aacgtcggtt cagagtgggt tatgcaaggg cggcaaggct gatagatacc
     7501 atggaagtgc tcggcattgt cggaccctat gagggaagca agccgcggaa agtcctcatc
     7561 accgaggagg atcttgaaga tgcgctcggc aatcgcgggt tagtatgagg ggcgcggggg
     7621 tgttgccgtt gaacgaaatc ggtgtgctcc ttagggaggc acgcgaggcc agtggcatga
     7681 gcctggaaga agtgagcgag gcgaccaaga ttaggtcgaa gtacttggag gccatagagc
     7741 agggagactt tgcggtcata ccgggtgaag tatacctcaa gggttttatg cgcaattacg
     7801 cggattgcgt ggggctcgac ggcgggcagg tagtggcgag atacaatgag cttagcctcc
     7861 tcaaggaaac agccgcacta aagcagctgg aagaagagcg cctggagcgg ctgcaaaaga
     7921 gcaaggagga ggcgaggagc cgccgggcga aaacgattct ccgcggcttg gttacagctg
     7981 ccataggggt ggcggctgtg gcaggtatct attatgcggc acgtgcattc gggtttgtga
     8041 cgtggaaacc gacatggctg ccatagcttg gagggatgaa ggaaattgag gagtgtgcag
     8101 gaactcctgg ccgatttggc caggctggaa ggcggccgtt ttgatgagac cctcttcgaa
     8161 ctggttcgcc gcggtcccaa agccctcgat aggctctttg cctacatcga ttctgacgcg
     8221 cctccaagca tctcagccaa agtgaatgaa tctattggaa ggcaatggac gagggttcag
     8281 gaggttctca tcgagctgcc gccaaaggca ttttcttcgc tgggcgtgaa agctctgatc
     8341 gaacgtgggc catctgacga aatcgcaagg cgtgccgcga agtgcataga gcggggcatg
     8401 atagagacgg ccgcgtgcat tgccactgtt ctctcctcgt cggaaaacgc ctgcattgag
     8461 cgggcaattc tggaagctcg tttgttgatg gcggcaggcc gcccaaagcg ggctatggct
     8521 cggttaagcc cttattgcgg gcccggagag gcgggaacaa tgatttcaga atggatggtg
     8581 gctgctgcca tggcagcggg ggagccacga cgcgcattcc tggcgatgcc ggtccgtgaa
     8641 cccgatgtca cacaggcttc gcatgcggta ggcgacatga tcgacgcgtg ttcagtcgag
     8701 gatctagcgg aatccatctt tgacgtggca cgccgaggag accttgcggg atgggagctg
     8761 atcggcgcgg ccacagaaga ggcatgggaa agggccgtcc aggatgatgc agatgttgct
     8821 ctggggttgg ctggcgcccg atcggaaatc gaaggctttc ttgatggaat cgaaagtgtc
     8881 ctttgggatc tggagtcatg tccagatgat gtatcccgcg ggttaatcca gaaagaactg
     8941 agttctttag ttgcccgtgc caatgaggtt gttcggacaa tcggagaggc gtatcatctt
     9001 gctgaaaggc ctgcgggtgg ctgcggcgcc accggagccc ctggtgaatg tggaggttcc
     9061 agttggatgc tgacggatgg agattgctgg gaccagatgt tggcatcggc caaggagagc
     9121 gttggtcccc agataatgga aagtctgggc aagtcggcct caagacagag gcttcccgag
     9181 gagattaacc gcgccgtgat gctggcccac gctgagggca gacccatatc gacccgatgg
     9241 gcggagggtg agtttttcat cgaagttgga ctccccatga cggagaaatc acctgaggcg
     9301 tgtgagggtt cactttctcg gctcagttcc acctgtgcct atggaggctc tcagatagag
     9361 agcagccttg atgcgggttc aatagcgagg atgttaaggt cttctcaacc ccggcagtcc
     9421 gcttgggaac tgatttgggt tataagggat cgggagcgcg cacatgcaac agctctgctc
     9481 ggcctggttg ctcagattct ggaggatatc tcgcacggcc tgaacaacct gtcggggcat
     9541 tcgccagcgg ctgaaggagc gcagctggag aagatccgac aacggcttga gcgctgggat
     9601 gatgaagtag aaggttgtgt tgccaaagcc ttgcgcaatg tggaagacaa gcctgagcag
     9661 tctgaggatg gcggtctgtt cggcccggac cggccgcagg atttgtgctt cagtccagag
     9721 tcggcacagg cgctggcggc tgctgactat ctgcttcgct cggcccgccc gagtgttcca
     9781 gcgcccgagc aggcacagct tatcagtttc ctgatccgga aggcagtgca gacagaagcc
     9841 gaatgtcggt tcgatgaagc tttggcagac tacaggcttc gtccggcggt cctcagagcg
     9901 agtgaagcaa gggggaaaag acgcagaaaa atctcgcccg cattcgcaga acgggcagtg
     9961 aagttggcgc ctgagtgcat ccctcctgat cgcgtgtttg ggggcttgga tcgtcttgct
    10021 tcgaaggtga tcgatggcag cccaaagcct atcgccgggg acatgtttct gatggctttg
    10081 gccttcatgt gtttcgatcc cttgggtgat cccgatcgtt gctatgcgct cggcagtgtc
    10141 cttgcgcgcc tcgatgctgc ctgtcaggat gctgagaggc ggcccgatgt gtgtgattgg
    10201 cccgggctgg gccgcctagt gtgtgatgcc ataagcctta tgagcgcggc aggtgatgaa
    10261 ttgtgattga gccagcgcat gtgaagggcg tggactactt gttgactgag gcggcaaaca
    10321 ttgacatcga ctcccttgtt gacctgttca atgatgccta cagccagtat tacgtttcga
    10381 ctgtggtcac gcctgagcgc ctgatcaagt tggttgaccg tgaggacata tacatgcacc
    10441 actcatttgt ggctctttac ggatcccgtc ccgttggagt cgtgtttctt ggagtacggg
    10501 gagaacgggg ctatatctgc ggaatgggag tccgcatagc ctaccaggga cgcggcgtcg
    10561 gcgaacttct catgaggcga gtattgaaag aagcggagac actcggtctc aactcgctac
    10621 agctggaagt tgtggatcgg aacttcagag ccataggcct ctacaccaag ctagggttcg
    10681 tgcccattag ggatctggga atatgggaaa gaaatcaaat gcctcgcaga aagggcgctg
    10741 ggagatggaa cccaaaaggt ttcactttaa accatgtggc ttcatttcag gccctgccac
    10801 tcgtgatgga gtttaacgag atacggccgt gttggcagaa cgagccaacg ggtctggcga
    10861 agttctgcag cacattgtca gcgctcgttg cacgagcaga ctccgaaaaa atcgcctatg
    10921 ctctgtatgt cgataccgtc gaggggctct acatagtgga tttcgccgcg acgccttcac
    10981 ttgacgaagg aacaaggcag aaagcatgtg aagttcttct ggaagccatt gacaaacaaa
    11041 tgccaaggcc gacggcgcgc gcacacaata ttcccgtcca cgggtaccaa ggacgtgtat
    11101 tgcggggaag gggattcgac atagggctta agcagcagga gatggtgttg tggttaaagt
    11161 cgcgttgacg acctttgtca ctgtattcct ggccgagctg ggcgacaaga ctcagctggc
    11221 caccatgatg ttggttgccg aaggcaagtc gccttgggca gtttttctgg gttcagcatc
    11281 ggcccttgtg gtttcctcgc ttgttggagt gcttgcggga ggattcatag gcaagtatgt
    11341 tccagccatg tgggtgagga taggggccgg atctgccttc ataatactag gcgtccttct
    11401 tctcatgggc cgattctgat ctcacaatac tacgaaacca gggggtgtaa tgttgacgtg
    11461 aaggaccaaa accgcattca caggcttgtc ctagtcgtat gttaccttgc gatcgtttgt
    11521 atggctatgg tcggtctttc aggtgctgat gcaacattgg cgcgtggggc gattgaagtt
    11581 gctgatcagc tctactcgag gagatccgag cctggaaacg ctgatgccat cattgcggga
    11641 ttggaacaag ccaacaaggc tacaccggga gattgcgaga ttctgtggcg gttgtcacgg
    11701 gcgtattggt tcaagtctac gcggcttcct gaaaaggaca agaaggcaag gctcgacttg
    11761 ctagctcagg ggcgagccta tgcccaagag gcaacagaag ccaatccgag gaatgcgaac
    11821 gcgtattact ggcttgcagc ttcgattgcg caggaaggac agataagggg gattttgcaa
    11881 agtcttgcct cggtaaaacc tacgaaggac gcattggata aggggttgga cattgatccg
    11941 aagcatgcaa gctgccatta cctcatggca gagctgtatc tgcagcttcc tgggcctccg
    12001 ctttccatag gcaataagca gaaggctgtt gaagaggcga aattggcagt gaaatacgac
    12061 ccaagcgagt cctgccatca tctcgttctt ggacgggccc tgatagcgac gaagcaatat
    12121 gatgaggcca gagccgtctt gaacaggctg atcgccatgg cgcccgatcc gggagatccg
    12181 gagggatttc ggaaggatca ggatgcggcc cgtgcagaac tgaaatcgat cgcaggaaaa
    12241 taatctcctg cacaaagaag cattgatctg tcgaaggttg gtgacttagg aacgaagatc
    12301 gcgttttgct cacttggatg ttcaaagaat cttgtagata ccgaaataat gatggggctt
    12361 cttcgcgacg ccggaatgga gattgtgtca gacgagcatc ttgcagatgt tatcctcgta
    12421 aacacgtgca gcttcattgc tgatgcagcc caggaatccg tcgatgctat cttggattat
    12481 gcagatctaa gggcggatgg gaagttgcag gctctccttg tcacaggctg tctgccacag
    12541 agatacggcg tggaactgat gcgggagatt cccgagattg atggcatcat cggcaccgga
    12601 cagttcggtc gatgcgtaga agctgttgag gacgccgttg ccggcagacg tcctgctttt
    12661 cttgaggatc cagggtacct gcagccggcg gacgctcctc gagtggtgac tacgcctgct
    12721 cattatgcgt acctgaagat cggcgaaggg tgtgcgaaca ggtgtgcata ctgcatcatc
    12781 ccgtcaattc ggggtggggt gcagagccgc cctctagagg cggtagtggg ggaggcggaa
    12841 aaactagttg cctacggcat gcgggaagcc atcgttatag cccaggacac gaccgtgtat
    12901 ggagtggatc tgtacggcgt gccatcgttg gcaaaactct tgcgaaagct cgctcgcgta
    12961 gaggggcttg aatggataag ggtgatgtac gcgtatcctt ccagaataga cgatgagctt
    13021 gttgaggtca tggcgtcaac gccaaacatc gtgaaataca tagacctgcc gatgcagcat
    13081 ggttcagata ggttgcttcg ggccatgaac cgacacgcct cgcgcgatca gatgatcgaa
    13141 gtggttgcgc gcctgaggga gcaggtgcct ggaatcgtca tacgcacctc gctcatcgtg
    13201 gggtttcccg gagagacaga tagagatttc gaggaactac tggacttcat agagataatt
    13261 aggcctgagc gagctggagt atttaagttc tcccgtgaag agggcactcg ggcctacgac
    13321 atgcctgagc aggttcccga tgacgtggct gaggcgcgca gggatgctgc gatggaggcc
    13381 ttgaggcgga tctcccgaga atacggcctt tcgagagtgg gcagtaaggt tcgggtgatc
    13441 gtggatgggc cgtctgacga gtctgacctc gttgtggaag ctcggtcttt cgcggaagcg
    13501 ccggatgtag atggcaagat atacataggc gatgcaacat tgcggccggg tcaacttgtt
    13561 gatgtgataa ttgaagacgc aagcgactat gacctggcgg cggtattggt gtaagggcgc
    13621 ggtctgccac aggtaggcgc gcaccgtagg ccaagccgct tccattagcg ttacaggagt
    13681 cgtctctgag ctttgccaca atagaagatc aggtgagaat gcgccgcctt ctgagccgat
    13741 gatcggcccg gaaggcggct atgttgtggt ggaaattcgt gttctgcact ggagctctaa
    13801 ttacgcaacc tcgggctgaa actgcaggcg taggcgagtt tctcggcatg accttggtaa
    13861 acacgtaaat catcatgatg ctgcaggtcg gctacttctg ctgccaactg tcatttccga
    13921 gggcaagcaa atcgcctgag caatgtcttc acttccacgt tgcgtgccca taggaatcgc
    13981 ttggatcggc atgccgagca tcacgaaata cagcccccca gactttttcc gccgtcagtc
    14041 cctgggggca aattcgtgat tggatacatc caacggcgct ctgcggatgc taggcactaa
    14101 ccaccgcgca gctgtcgcat ggagcaccat gtcctgtgtg accgtttgcc gctaggggga
    14161 agctactttg tggaggtggg ggagttgatg aacgcacagg ttgccgaggt cgtggacgtc
    14221 gtttccgagt ttcctgatct acaagagctt tcggtaaagc tggccgaaaa tggatgtaca
    14281 gttcgggcaa tatgttaccc taaaatcaca ggcactgcac agccaggcga tgaggtgatc
    14341 gtgaacacca ctgcggtcga cctcggactt ggaagcggtg gtactcactt cgttgtgtgg
    14401 gtgttgggga gggatcgttt gagcaatcag ctgcctggtc acataatgaa gatgaggtat
    14461 actccgatgc aactggctac agggagctac gaagagaatc cagcgtaccg tggggcaatt
    14521 gacagttttt cggggctaga cggcatgcct gtggtcatat gcgagctcca ttcgatggtg
    14581 gctcccgtcc ttgccgggtt ttcaggaaag gctgcttaca taatgacgga cggagcagcg
    14641 ctcccatacg ctttgagcaa ggtggtccgc cgcctgaaag aagaaagact attccacgtc
    14701 tcgattactg ctgggcacgc cttcgggggc gatctggagg cggtcaatgt ccattcggcg
    14761 cttgtggctg cctatgccgt atgcaggtgc gagggagcgg tggtggcgat gggcccgggc
    14821 atagtcggaa caggcactca atatggcttt tccggcatag agcaggcaca catagctgat
    14881 gcagtcaaca gaatgggggg gcgtgcaatc atagtgcccc ggctttcaag atgcgatcct
    14941 agggaacgcc atcatgtatt gagtcaccat acagtcaccg tgttggcgga cgtctgttgc
    15001 acgccggcta catgcgtgtt ggcctccgat atggatgacg gattccgccg gaaggtggaa
    15061 gaacgtctct gtgggatact gaaagcaggt gggcatcggt tggcttatgc gcctggagga
    15121 ggagccatag acgaactgaa ggagcggggg atcgaactct caacaatggg gcgcgggttc
    15181 gacgaggagc cggagttttt cctcacctct gcggcggcag gcgcgtatgc acgaaaactc
    15241 gtccactagc tttgcaaaag tcttcgggcg ctcccgtgta acttgggcta gaggcagatg
    15301 gttcgaatga acagataggg tggaaggctg accgggagtt gcctggggat agtgcttcat
    15361 ctgtccgaat atgtccggca tacggaagaa gacagttgag atggtcgggg tctacgtcct
    15421 ggtttttgca tgtttttgta gctacaatgg ggctgtatga tccgcggagc atcctggtgt
    15481 ggcggggggg agttgccgag cggcgattgg aagagcaaag gccgggaaat cacagagagg
    15541 ccttgcgaaa actacatgga tgttcaggag gcagatatca atgaaacaag accttactga
    15601 aaaagctatt agcagtgaga cgatctacaa cgggaagatt gtgactctta atgtggacaa
    15661 ggttgaactt ccaaacgggc gcacggccac gagggaagtc gtaagaacgc gggattccgt
    15721 ggcagtggta cctgtgacgc gtgatgacaa ggttgtactc gtgcggcagt acaggtacgc
    15781 agggggtgaa gagcttcttg agatcccggc cggccgtatc gatccgggag aatcccctag
    15841 ggcggcagcg caaagagaac tgcttgagga gaccggttgt acatgcgccg aactgaagaa
    15901 aattgcaggc ttctatattg cggtcggctt tgccacggag ttcatgcacc tgtatgcggg
    15961 aacagttgac cacattggcg aggcctgccc cgatgatgac gaattcgtca gaacggagct
    16021 gatgccggta agccagatac ctgagatcct ggcaaagcag gagataaggg atgtaaaaac
    16081 ccttgccggg ctgcttcacc tttcgtggat gttaggcctt tctcctgtca gctcatgcgc
    16141 ttgcagcata tgtcgtgcgt aatgccaaaa tccaatggga taatctgatc gatcaatact
    16201 gactgcaatt ggggatgttt acataatgtc agaacgaggg ctcaaccgct actttgtcga
    16261 ccttcacgtc catatcggaa gagattctgc aggtaagcct gtaaaagttt cgggtgcgcg
    16321 caatctaacg tttgccaaca tagcgaagga ggcagccacc cgcaaaggca ttgacattgt
    16381 cgccgtcgta gattgtgcat caacaagggt tctcaaagat atagaggccc ttcttgaatc
    16441 gggcgacatg accgaggcgc ctggcggagg gtttagatac ctcgacaaga ctacggttat
    16501 acccggtgca gaggtggaat ccgcctgtcc gggatcggct agaggcgccc actatctcag
    16561 tctctttcca accttgactg ccttgcgcca gtattcgcgc agcctttcac gatatgtgtc
    16621 taacatggat ctttccaccc agagagcgcg gttatcgcct gtggagcttg cgcagctcgc
    16681 tgccgatagt ggaggacttt tcttggttgc ccacgcattt accccacaca agagcctgta
    16741 cggaacctgc gcccgcagaa tgtcagaagt gttcggagag gatgtgcgaa aactagttga
    16801 cggagtagag ctaggacttt ctgctgacac tgacatggcc gacaggatag gggagctgtc
    16861 gcactataca tttacctcga attcagacgc ccattcgttg ccgaagattg gacgggaata
    16921 caacgtcatg cgtatgaaag cgccatcctt ttccgaggtc cggctggccc ttgcgcgtct
    16981 cgatgggcgc agagtcgtgg ccaactacgg cctcgatccg cgtctcggca aatatcatct
    17041 cacagcgtgc gaagagtgtg ggcatcgatt caaggcatgg ccaaattcat gcgcttgccc
    17101 cgtctgtgga agcgttcgga cggttcgggg tgttgcagac cgaatcgacg agctttccga
    17161 tactgcggaa ccgattcatc caccccatcg tccttcgtac aattaccaaa ttcccctgga
    17221 gttcgtgcca ggcctgggac cggcgagaat aggggcgctt atcgacgcct tcggcacaga
    17281 gatgaccgtt atccatgaga ctatagaatc ggacattgca gaggtagtgg gggaggagat
    17341 tgcacacagg atatgcttgg ccagggaagg gcgtctcggc attcaatctg gtggaggcgg
    17401 tgcatacgga aaggctgtag tcgaatagat ccatcttggc attgatcctc cctgtagcgc
    17461 atatccatgc tctatggagg gggtgtacaa gatggccaga gcacgtcgca catcccggtc
    17521 tgtgttgagc catgctcgtt cgaatatggt tggatactgg ctgacgttcg tagtattcat
    17581 tacaggagta agctgtggcg cagttcaagc ctcgatgttg aaggaaggca gaaccgagct
    17641 gttctcttat cttgaggccg gcctggccag cctgagacat acacttcctg ttgcgcccgg
    17701 caaggcgtta acatatagcc tgggcgcgaa tctggcttcg gtagggctca tatggctttc
    17761 aggcctcacg atcctaggct tccccggggc acttgttatt cttttcgtcc gcggtttttc
    17821 catcgggttc accgtggcct tcatggtgcg cgagttgtcg gggccgggcg cggcacttgc
    17881 tgtggcatca gtgcttcccc ataatatcct agctgttcca ggcctcatac tcgcctgctt
    17941 tgtctccatg tcgttttcat gggcggtaat cggaaccaac cttataggca tgccttcaga
    18001 tgtgaaactt gccttctctc gatcggttgc aggggtggca atctctgcta ttctcctgtc
    18061 ggcggcctct ttcgtccagg cttatgtatc cccggcaatc ttgatacttg ctgcaagatt
    18121 cgtctaatca actcgcaaca cgtgtttttg cctaattgag cggctagtgc gcagaacaac
    18181 gtcttccgcg aattcgaggg ccgacttcac ctggcttctt atggcgaacc ctcctcaatc
    18241 cgttcactct ttttccgcat gtgttgccgt gcgccagctt tctcgtgtct ttgccccacc
    18301 gaggcagaac aaggagacat tttggatctg cggccgccgc tattcggcct agatatgggt
    18361 tgtggttgca gaatggggat ccgtggatcc agagtctggc ggcccgcgca tcatcgggct
    18421 aagactcttc cgcaaagcct actacaaggg tggggattgc agaattgtat tggaaaagac
    18481 atggactgtg atgctctttg cctcaggatc catgcaggac tacactgacc aacgtcgaag
    18541 agaccgttta gaaatcggac gatatggata tgcagatacg ggcggaagtt attatcctgc
    18601 atgaatgggg acgcaccgaa ttaatgcatc agctcgtgag ttggcaggcg gcatagcttg
    18661 ccgctcctgc tgtagcggct atagatcgaa caggagggag tgttctgtgc ttccatctgt
    18721 agaagaatac atcaactatc tcgcggttga acggggtcta tcggaaaaca cgctagaggc
    18781 gtacgcccgc gacctctcgc agtttgcgga gtttgcggcg aaatccgggc gtcaatcaga
    18841 tgagcctatt gatgacgata tccttacggc gagtcaatcc atggtagcgt cgttcattgc
    18901 acgctccata gagtcaggcg gatcaccttc atcagctgcg aggcgactta catcactacg
    18961 cagatactac agatttatgc tacaagaagg atacgtgaag gcggatccca ctgcgaacct
    19021 ggaatcgcct cggcagccac agcggctgcc tgacgtgctc accgaggagg aggtaaacag
    19081 gttactctca tcggcgccgg ggtatgatcc tcagtccctt cgcgacacgg ccatgttgga
    19141 actgatgtac gcgtctgggc tgagagtctc ggagctgatc tctttgacca ctgaagatgt
    19201 agacatggag cttggttacg tgcgctgtgt gggcaagggt gaccgagaga gaatcgttcc
    19261 agtgggaaga cgcgcaatag aggccataga gagatacctt gctttcggac gccgcgaact
    19321 tgccggcgtc tgcaaggata actacctttt cataacgaac cgcggaactg ccatgactcg
    19381 gcaggggttt tggaagatga taaaacgccg tgcgctcaag gtgggcatct ccactgacat
    19441 cactccccac acgctcaggc actcattcgc cacgcacatg cttgaaaacg gggcggactt
    19501 gaggtcggtc caggagttgc tgggacacgc ggatatccgc actacgcaag tttatactca
    19561 cttaactcag gtgagactga gggaggtata caataagcac cacccgaggg cgcttgggga
    19621 aggagacgca tgagtatgcg agcactgatc atcgtgtttg acggtgtagg gatcggagag
    19681 cttcccgacg ccggtgaata tggcgactta ggaagcaata ccttggtgaa tacatcgcgt
    19741 gcagtaggag ggttgaatct tccgaacctt gaaaaactgg gattgggcaa ccttgcggca
    19801 gctgccggac atgagatttg cggagttgcc gcaatcgagc atcctctggc ctcgtacggc
    19861 acaatggccg aaaagtctcc cggaaaggat actacgaccg gacattggga attggtcggt
    19921 atacagctgg atcaggcatt ccccacgtat cccaaagggt ttccgcccga gatcattctc
    19981 ccgttccagg aagctatagg caggaaggtc atcggaaata tcccggcatc gggcaccaag
    20041 atcatagagg aactaggcga ggaacacatc cgcacgggaa gcccaatagt ctatacctcc
    20101 gccgattcag tctttcagat tgccgctcat gaagaggtga tcccaccggc cgagctgtat
    20161 gagatgtgca agatcgccag ggaattgctt tcggggcctc acgccgtggg cagggtaatt
    20221 gcccgtccgt tcatcggcgt gcccggcaac tttatccgaa ccgaaagaag gcgcgatttt
    20281 tcactggtgc cgccagggcc cactttgttg gatctcgttg cccgcgaggg gctagaggtg
    20341 atggcgtgcg gcaagataga cgatatcttt gcgaaccaag ggattacgcg ttcaaagcac
    20401 gtggcaggaa atgacgccat attcgatgct gtcctcgaat tccttgacag tgggcattcg
    20461 ggcctcatat tcgccaacct catagatttc gacatgaaat ggggacaccg caatgatgtg
    20521 gagggctttg cgcgcgggtt ggaacatatc gattcccgcc tcccggaact ccttgaaaga
    20581 ctcggtgaat cggaccttct ggcgatctgt gcggatcatg gcaatgaccc cactacgcca
    20641 agcacagacc attccaggga atacgttccc gtgctcgcat attctcctgc caggcaaagg
    20701 aagggacgat gtggcgccca tgtgggcgtg agggagactt tttctgatct cggtgcaact
    20761 gtcgcggatt ttctcgaggt aggatggaca ctgaggggag agagctttct caacgaaata
    20821 ctgtagctgt gcgcggcaag agcggaggag tttaaatgag agtcccggat ataatcatga
    20881 aaaagcgaga cggtggcgaa ctgagcccgg aagagatctt tttcttgata gacggcatgg
    20941 tttccgggga tgtgcccgac taccagatgt cggctttctg catggccgtc tattttcagg
    21001 gcatgacgtc cgctgagacc actgccctca ctctggccat ggcaaggtcg ggagagatag
    21061 tggatctttc ggctattcgc gggacaaagg tagataaaca cagcacaggg ggcgtggggg
    21121 acaccacgac actcgtgctc gctcccatgg ttgctgcaac cggagtgccg gtggcgaaga
    21181 tgtccggaag agggttgggc catacaggcg gaaccctgga caaactcgag tccattccgg
    21241 gaatgcgggt gtctttatca gaagaggagt tcatcgagca ggtcaaccgc attggcatcg
    21301 ctatagttgg tcaaacagag gacgtggccc ctgccgacaa gatcctttac gcgctgagag
    21361 atgttactgc cacggtcgag agcatccctc tcattgccgc cagcattatg agtaaaaaac
    21421 tggcaacagg agcagatgca atagtcctcg atgtgaagac aggaacgggc gcgttcatgc
    21481 acgacctttc tcaggcagag gcactcgctg gccttatggt ggacatcggg cggatggccc
    21541 ggcggaagat ggcggcggtg atctccgaca tgaaccagcc ccttggccat ggggtgggaa
    21601 atactcttga ggtaattgaa gctgttcaga ctcttgccgg cgaagagcgc gggcgccttt
    21661 ttgatctctg tttggagctt ggggcaagga tggtagtgat gggcggcgca gaatcgagca
    21721 tagaggcagc gcgcgtgaga ctgaacgatg tggttgattc aggagcagcc ctcgagaagc
    21781 tgcgcgagat ggtagtagcc cagggcggcg atccagccta tattgacgat ccaaggcgtc
    21841 tgcctgttgc cccctactca gagtatatca ccgctccctg cagcggatat gtggcctctg
    21901 tcgacgcgat gacccttgga agggcagcga tgtccatggg cgcgggccgc gctcgaaagg
    21961 aagatagcgt agatctcgaa tgcgggttgg tgactttggt ctcaatcggc gatcctgttg
    22021 aacaaggaca gccagttgca aaactgtacg ccagcagcac ttctctgttg acggaaggac
    22081 tgtcagtggc ggctaaagcc tacacttatt ccgccgaacc tattgagcct ccgccgctcg
    22141 tgtatcgaat catagagtcc tgaatgcggg aactgtataa cagggcatgc ctccgaatat
    22201 gctcttttga gagaagtgaa ggagggaggc atgcttcttg tcatacattc gctgcggtag
    22261 ggcatgtagg gcggcacttg ctctcgttgc cctgttgtta gtcgtcaagg tagggatttg
    22321 cggcgcgcag agcgcccctc ttgctgccat gtcgctggat cttgacaaga aaattacggc
    22381 tcccagcgcc atactgatgg aggcaagcag cggacgtgtc ttgtgggaaa agaacgctga
    22441 tgtggcacgt ccgatcgcaa gcgtggtaaa ggtgatgacg ctcacccttg tcatggaggc
    22501 tcttgaggca ggtcggatag atttagacga catctacatc acttccgagc acgcagcctc
    22561 catgggggga tcccagatct ggcttgaacc tggagagtct atggctgtat ctgaacttgt
    22621 gaaggcggtg gccatagaat cggctaatga tgctgcagtc gctctagctg aacacgtggc
    22681 cgggagcgag cccgcctttg tggccttgat gaacgagaag gcgcaggctc tgggatgcaa
    22741 gaatacgcat tttattaacg caacagggct tcccggcaag agcccaagcg aagactgtgt
    22801 ctccagcgcg cgggatgtgg cgataatgtc ccgcgaactc ctcaagcacc cgcgtattca
    22861 tgagtggctg acaatatgga tcggctacgt cagggacggt cagaacatgt tgaccaacac
    22921 caataggctg atacgattct acccgggcgc ggacggagtg aagaccggat ataccgaaag
    22981 atctaggtat tgcctctcgg ctacggctgt gagaaacaat gtcagagtca taggggtagt
    23041 gctcggtgtt ccaaccagcg ctgtcaggtt ctctgaagcc tctacgttgc tcgattacgg
    23101 tttcaagatg tgcgaagctc acgtgatcgc ccgctccggg caggaaatgg ggcgggtccg
    23161 cgtcctgcga ggaatcgata ccgaagtcgg cgtcacggtc gattcagatt tcacagtggt
    23221 gactttaaga ggagaaggcg atggggtcga tgccaggctg gagcttcccc aatatgttag
    23281 cgctccggtt gaacgggggc agcgtgttgg caagattgtg gcctatgttg aaggtgtgga
    23341 gataaactcg gctgatctag tggcggtcga tgacattccg aaagcaggcg cgtttacctt
    23401 ggttgtaagg tcgacgaagg aattcgtcag gtctctcttt gattttcgct gattctgtcg
    23461 tgcccttgtc ggccattagc tggaaggaga agccgctttg gcagagaatc tctgagtcaa
    23521 gccaatgggg aggcggggga tggcggttga tcgaatggga ggtttctgga aacagtcttg
    23581 tcgtagagat cgccggcgag ctcgacctga cttgcgctga cgagtttcgc agggttctcg
    23641 attcattaat agactctcat ccaggtgttg cccgggtcat actgtgcatg gaccgagttg
    23701 cgtttattga cagttctggc ataggggtgt tgttcggcag gcacaggcga atggcattgc
    23761 gcgggggaac gctcgcgata gcctctccga gcccgcaggc gatgcgggcc ttggaccttc
    23821 tgggtgtgcc ttcggttata catgtctatg acacactgaa gcaggccctc gcggcggaag
    23881 cggaggtcag aaaatgcacg cgaaagtgaa ccatgcaaag gttgagtttg atgcgatccc
    23941 cggcaatctc ggctttgccc gcgttgccgc cgccagcttt gctgctggac tcggttttac
    24001 tgtagaagac cttgaagaca tcaagcttgc cgtctcagag gcagtgtcga attgcgttat
    24061 tcatgcttac cctggtcctg gcgaagcaag gccgacagta ttgatggtca tggaggcacg
    24121 tgaatcagag tttgtggtct ccattgaaga caagggaata ggcatagctg atgttgcaat
    24181 ggcgcgaaag cccggcgtga ctactgactc cgagagactt ggcatgggat ttaccttgat
    24241 ggaagccctg gctgatcagg tcgacgtaaa gtcggaactg ggcgtaggca cgcgtgttga
    24301 gatgatcttc tctccggagc ggggaggatg atgtggaacg ccgatacgca aggcgaatct
    24361 gcatttttgt catcccgcga agccgaagcc cggcgacacg cggggcttgt gatgtcgttg
    24421 gcacgaagat tcgcgggtcg ggcggagatt gcagatctct accaagcagg atgtgttggt
    24481 ctgctccgcg caatcgaccg gtacgaccag tcgcgaggca cgactttcag cacttttgct
    24541 gtgcctatca tactctccga aatagtggcc tacctgagag gctcaggtcc catccacgtt
    24601 cccaggcctg tgcgtgagct cgcgcagaaa tgtcgccata cacaggattc tctcagggcc
    24661 gaactttcca gggagcctca ggcaacagag atcgccgctc gtctggggat agacgcagcg
    24721 gatgtcgtct atgccatgga atctgaggca catgtctact cccttttcga ccaggtaagc
    24781 ttcgatcatg aatccctcga attgcacgag gtccttccag gcgatttggg cgttgaaaca
    24841 tcggtgtgtg aatccattgc cctctccgat gctctttccg ccttgtcaga aatcgagcgc
    24901 acggtggtgg cgatgcgctt cttcgaagaa cgcactcagg ctgagacagc cagcttgctg
    24961 ggaatttccc agccccaggt gcacaggatc gaaaaagccg ccctccttgc attgaggcga
    25021 agcatggaag gtgaatgaaa cctccggccc ggttgcatac tagcccatgt gggggtgtgc
    25081 atcttggcaa gtccgggagc aaagaaacag aaagctgacg aaagagcaaa gtaccagagg
    25141 ctcgcggcat ctatcgttcc caagccccct cttgtccgaa acatggtggt ggcattcgtt
    25201 gtgggaggcc tcatctgcac agtaggacag ggtgtgatca acgctttcat gtcccgtgga
    25261 atgactcagg acgaggcaac cgcacccacg ctggccacta tgattctgat aggctcggta
    25321 ctgacaggcc tttctgcata tcaccatata ggcgaattcg cgggtgctgg ggcggccatt
    25381 cccattaccg gatttgccaa cacaattgtg gcggcagcca tggacttcaa gcgtgagggc
    25441 tacatattgg gcatgggcgc caagatgttc atcattgcag gcccagtcct cgtatacggg
    25501 attctctccg gcgtgctggt tgcgctcatc aggtggctct acagggggta agctatgacg
    25561 acaaaacgtg taggcggaag cacaatagtc tattccactc cccctgtaat tgttagcgct
    25621 gctacagttg ccggcccaaa ggaaagcgag ggccagatgg ctcagtggtt cgacgagctg
    25681 gtgcccgatg acatgttcgg ggagcgtacg cccgagaaag cggaacggcg attcatggaa
    25741 gatgccatta agatcgccct gggcagtgcc cgtgtccttc ccgaacaagt caatatgata
    25801 ttcgccggcg atctgctcaa tcagataatc acctcaagct tcaccgcaga gggttttggc
    25861 attcctttcg ttgggctgta tggggcttgc tcaactatgg cgcagagctt aaccatggct
    25921 tccgcaatgg tggacggggg ttatgcggac cttgcccttg cggtcacaag cagccattac
    25981 caaaccgctg aaaggcaatt tcgctatccg atagagctga acattatgcg aaagagcact
    26041 tcgcagtata cggtgaccgg ggcaggcgcc gcattggtgg gcagatcagg aggcaaagtc
    26101 aaggtcactg aggcgactat cggaaaagtg atcgacatgg gcataaagga ccccaaccag
    26161 atggggcccg ctatggctcc tgccgcggcc gacactatct tcgcacacct tgccgatacc
    26221 ggacgggacc ccggatacta cgatatcatc ctcaccggag atttggggcg cgtgggagcg
    26281 gagatacttt gccaactgat ggcggagaaa ggggtgcccc tcggggacaa ttactcagat
    26341 gcaggcatcc tcatgttcga gggtgtaaga ggtactgggg taggggggag cgggtgtgcg
    26401 tgttcagctc tgatcaccta cgggttcatc ttgaagagga tgtgccagaa cgagatatct
    26461 cgggccctcg tcgttgccac aggcgccctc atgtcgccca tctcctggca gcagggagaa
    26521 tcgattcctt gtattgcaaa tgctgtcgtg ctcgagacgt aatcgccatg ttcaggtcat
    26581 caaggcccat atattgtagc cttagggagg aactttggtc acatggacta cctcgtggcg
    26641 tttcttgtag gaggaacgat ctgtgcaata ggccaggtgc tgctcgacac gagcaagctt
    26701 tcacccccgc acatgctagt agcctttgtt gtagccggcg caatcctctc aggtctggga
    26761 gtctacgata aactggtgga ttttgctggg gcaggcgctc taattcccgt atcaggcttt
    26821 ggtcattcaa tagcccgcgg ctcgctcatg gaggcggaaa ggctcggcgt actggggctg
    26881 ttcaccggtg ctctggagtt tacagggctt gggataacca cggcggtttt tttcggcgca
    26941 ttgattgctc tagttgccaa tcccaagcca tgaccatgag agtgggagtt ccccgcgggc
    27001 tcttgtacca cgagtatgcg cctgccatcc acactttttt ggagtcttgc ggagccgaaa
    27061 cagtcgtgtc gccgcaaacc aaccgggaga tactggagaa ggggcttgct ctgtgtgtag
    27121 acgatgcctg tctgcctgta aaggcttttt tcgggcatgc cgccgttctg tcggatcagg
    27181 ttgacgcctt gtttgttgcg aggtttgtga gcgtggagcg tggagcgtac atatgtccga
    27241 agttcatggg cctccccgac atgatcagag cgaggctgca aaatgcgccg cctatcatcg
    27301 atatgtgtgt cgacatccgc cagggcctcg gttcgtatgt ttcatccctg ccttgctcag
    27361 acacttgcaa ggtattagga gtggcaccac ggcatctatc atgggcattg ggcgccgcct
    27421 tgatggcgca gcgaaaggaa gaccgtcggc gcgtcaagag gtttgcgccc tcaggctctg
    27481 ccgtagcccg tacagggcta ggcggataca cgcgtgaaag catctatgaa acctcgttga
    27541 ccataggggt gttaggtcat gcctacaacc tcgatgacgc ttatcttagc ttgggcctaa
    27601 tatcaaagct tgagaagatg ggcgtgaagg cagtcacagc agaggattac gcatacgaca
    27661 atctgaggcg gtgggagagg cggggggcgg acagaaaaca ggtcttctgg actgccggcc
    27721 gcaaactgat cggggcagct cgcagatgga gagagacggg agaagtggac ggaatggtgc
    27781 acttgacctc ctttggttgc gggcctgagt catttgttgc tgaagtaatc gcccgagacg
    27841 cggcgcgcca tcccgccatg cctctaatca tcctcaatgt ggatgaacac tcaggcgaag
    27901 ccggcatggc aacgcgtctg gaagcgttcg tggacactgt cagaatgggg aagtcgaagc
    27961 gatgaaagtc tcctttccgc atctgggaaa cttgtggatt cccctgaaag ctttcttctc
    28021 aggaatcggc attgaggtgg ttgcgccgcc tcccactact cgaaagacgg tagcgatagg
    28081 cgcgcaactt gcccccgaaa cagcgtgcct tcctctcaag gtaactattg ggaacatgat
    28141 tgaagcgatc aacagcggcg ccgacacttt tctcatggtg ggtgggatag gtccgtgtag
    28201 gcttggctac tactgcgagc tgcagcgaga catactctct gaccttggcg atgacgtgga
    28261 cctggtggtg atagagcctc ctaggggcca ctttcgcaac acggtccggt ttgcacgcct
    28321 cctcactagt cgtgcgggcg tgagggatgt ggttgaggct gggaagcttg cgtgggaaaa
    28381 atgcataggc atcgacgtct gcgagcagtt ggccaatgcc gcgcgctggg gcgaagccga
    28441 aagaggcgcg gtagacagag ctgttcggaa gggacttgcc gaactggaca gagcctctac
    28501 gattagagac gtgcaagccg tcatcgccaa cacggtggaa cacgtacggt cctgcgccgt
    28561 agtaggcccg atctgtcaac tcgattcttg tgctcgaagt ctagtaacgg ataggtatgg
    28621 cgcccttctg cgttcggaag aaggcgatat ttctccgctt tctgttggag tggttggaga
    28681 gatatacgta gtgatggagc ccgcagtcaa catgaacctc tgcaagaagc tggggactat
    28741 cggggctcag gttatgcagc cgatcacgtt ttctgggtgg atcatgaccc atgtagtcct
    28801 cgatatagtg aagtttatgc caaaacctga tatgttggca tgggctacgc cgtatctggg
    28861 gcattttgtc ggcggccacg gcgtggaaag cgtggggcac tcggttcaga tggcccgaag
    28921 cgggttcgac ggcgtcgtgc acatctctcc actcacctgt atgcctgaga tcgtggccca
    28981 gagcatattg ccacgggtga gcgaagagga agggatgccg ataatgtcgc tgaccatcga
    29041 tgagcatacg gcagatgcag gcctcgacac caggttggag gctttctgcg atctgcttgc
    29101 agaaagatca ggcaaacgat tggacgggat ttcgggtgca ggtcgatgga aagacatgca
    29161 ggttctctga aagcccacgg gttcacgtca tttctcactt tcaaacaagc tgtgccgact
    29221 attgtcccgt ctcatcaatt gcacatctat taacaatgct ccattcannn nnnnnnnnnn
    29281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn nnnnnnnttc gcaatcaact acagctgaac aagcctgcgg
    29401 cgacggatgg tgttccccgc tagcggctgc gtatgttata atcaggccca gaggagggaa
    29461 agcccgtgct atcgatgaac ccttactcca tgctcattac gttgccgatt ttgctttttt
    29521 ctttgtcagt gcatgagtat gcccacgcgc gcgtagcata caggctgggc gacaacactc
    29581 cccgatggca agggcgcctc actctcaacc cccttgccca catggacccc gtaggccttc
    29641 ttgtgctcgt cgttacaggc aggttcggat gggcaaagcc agtggtagtc aaccccaata
    29701 actttgaaaa cagggatgcg ggaatggtaa tggttagcct ggcaggcccc ggttccaacc
    29761 ttgtgttggc aattgccttg gccacgttgt ttaaggcggt atccgggccc tatggcatac
    29821 cggcgaatgc atttgcgcgc tacctcgcgc tcgcattgaa ccagggcgta tggataaacc
    29881 ttgggcttgc cgttttcaac ctcctgccag ttccgcctct tgacgggtca aagatcctcg
    29941 cagcgctcct caagggcaaa ggcgcacgtg tgtactacac gttggagaag tactcttaca
    30001 tcgtgctttt gttgttcata gcgacgccgt tggcgagctg gatcctgagc ccgatcatat
    30061 tgacgcttta cagagcgata atgtagaagg tggtttgttt ggcaaaaggg cgcatactga
    30121 gcgggaacag gccaacggga aaacttcatc tgggcaactt cgccggagcg ctggccaact
    30181 gggtcaagct ccaggacgat tacgagtgct tcttcatggt agccgactgg cacgctttga
    30241 ccacgggata tgaggataca tctgagctga agaactacac ccgccagatg ctgattgatt
    30301 ggctgtctgc gggcttggat cctgaaagat ccgcaatctt caggcagtcc gacgtcaaag
    30361 agcacgcaga gcttcatctg cttctctcaa tgatcacgcc cttggggtgg ctagaaaggt
    30421 gccccactta caaggaacaa ctgcagcagc tgtcggggaa aagcataact aactacggtt
    30481 ttctgggcta tccggtgctt atggctgctg acatactggt gtataaggcc gatacggtgc
    30541 cggttggaga ggaccagctt ccacacttgg agttggcacg cgagatagcc cggaggttca
    30601 actacctata cacgcccgtg tttttggagc cccaggctaa gctgagccag gtaccgatcc
    30661 ttccgggaat cgacggacgg aagatgagca agagctacgg aaatacaata gccctgtcgg
    30721 atcctccgga aaaagtgcgc gagaaggtga atgcgatggt caccgatcct cagcgcatac
    30781 ggaagtcgga tcctggccac cccgatgtct gcaccgttta tgcttactat aaaggtctcg
    30841 cccccgaact gatcgatggg cgcaggcagg aatgtgaggc tgggaagatc ggctgcgtcg
    30901 cctgcaaaaa ggaactagcc aatgtgcttc ttgaaatctt aggtccaatc cacgaaaggc
    30961 gccacgagct tgaagcccag cccggcttcg tcgagtctgt cctcgaggag ggagcggcca
    31021 aggcacggcc tatcgcggtt cagaccatgg ccgaagtgcg ggaagccatg aggatatgaa
    31081 ggtagagacc cccgtcttct cgggccccct cgatctgatg ctctctctca tcgaaaagaa
    31141 ggagatagac atttgcgagg tatctatcag cgagattacc gatgactact tggaacatgt
    31201 atcatcgatg gagtggctcg acatagacca ggctgcagaa tttttggtga ttgcagccac
    31261 gttgctctac atgaaggtca aagctttgct tcccgagcag gaactcgcgg aagaattcgg
    31321 cgtggaagag gatgagttgc ctgaccccgg cgcagacctg gtggcacgcc tcatcgaata
    31381 taggcgctat cgcgatgcgg cgcgtgtcat gcggagcctt gaagccgcgg cttcgaggat
    31441 gtacacgcgc ccgccgcgtc cgcttccaaa gccggctatg gtgaaagtga acccgctgga
    31501 tggcatatca gctaaggatc ttttcgactt gtatgtcaaa gctgcaacga gtgttcccga
    31561 ggagttcacc catctgccgg aggatgagat tgacattgtg cctcagatgg cgtctgtcct
    31621 gacgcgtctg gcgcgtcgcg gacgcttggg ctttcatgag ttcctcgggg aggatgcgtc
    31681 ccgccctgtc atcgtagcaa cgctcctggc catgctggag ctggttaggc ggggaaggat
    31741 ctctgccact cagccaatgc ctttcggcga gatatggatg ttcccgcaga aggaaaaggc
    31801 gtcatccgga ggtgtgtccg acgaatctcc aagaagcgca agcggcaatt gaagcaatac
    31861 tgttcgcggc ctcgtttccc ctccgggctg aagagatcgc atctgccata ggggttgatg
    31921 agtcttcgac cagaaaactc ctccggttga tcggggatag acattccagc ccagaatcgg
    31981 gtgtagcctt ggtggaaact ggcggcgggt tcatcatgtt gtcgcgtccc gagtacgctc
    32041 cctacataga acggctttcc gaaagacaac gtcctccgtc gctttctccg gcttcccttg
    32101 agacactggc catagttgcg tatcggcagc ctgtcaccag gggagaaata gagagaatca
    32161 gaggggtatc tcccgattca gccatatcca cgttgctcga gcgagggctt atctgtgaaa
    32221 agggacgcaa ggagagtata gggcggccga tcctttacgg aactacgccg gatttcctgc
    32281 tttacttggg gctcagctcg ctcgatgatc ttccggtgct tcccgatggt gaagggcttc
    32341 ctgcagacgc gcctcaaggt gccgatgagc aggatgcgtt gatgtcacct cttcctggct
    32401 tggggaagga cggcgatgag gctggaggca atgcgcattg ggatatgcca gatgggatcg
    32461 atcttgagtc aatgttcgac ggtatgagcg cgacagatgc caactgtgat gaagaagacg
    32521 actgaccgag tcagggtcga ccgaagaatc tgggcgttcc gggcctgatt tcacgcgatg
    32581 gtgaatactg ctcctgctgc gcggcagaat acgagcggga gtggtgcggg tgagatgggt
    32641 tgtagcgctc ctttcggttc tggcaatcgt tgtcgcggcg cttgtggccc tgattttttg
    32701 gcccataagg ctgaggttgt cagcccattc aggtgaaatg tgcgcctcgg ttgcggcgcg
    32761 cgtttcatta tggccccatc tattctcttt ccaggtctac agccacgaca tcattcccgg
    32821 aaccagccaa cctcacaaga cccccactca gcgcttggct tccgtaaaag agaccataga
    32881 taggtttcaa gctcttgttc cagtcctcag cgcggcgctg tctgggcacc acaagcttat
    32941 cagtgtcgtg tggcattccc tggtcggtac gggcgatgcg gcgctcacag ccatagcgtg
    33001 cggaagcttg tgggccgcaa agtcaacgct tttatcgagc gcatacgcga ggtggggcaa
    33061 aaggtccgac ctcccagaat actcagtcac gcctctcttc gaccgccagt gttttctcac
    33121 agatcttgac atctccctgg aaacaagtat tgcatcggta atcctcgctc cgtcgttctc
    33181 ctctgctgtt atggggtttg taaaagctgg ccgcaagaga agggaatgaa ggcagaggca
    33241 gcgatcaata ataagcagag aatgctgttg aaagcaaaaa agcaacagga ggaatgcatc
    33301 ttgcctgagc atcctataga aggcctgatg aaaacgaccc tcgaaagcat taggcagatg
    33361 atcgatgtga atacgatcct tggcgaccca gtggaaacta aggatggcaa cgtaatcatg
    33421 ccagtctcaa gagtgacatt tggattcgcg tccggcggca ctcaatggga cgctagtcgt
    33481 cgccgggacg ggggacagcc gccgaccggc tcgggtggcg gcaccgctga actgcctttt
    33541 ggcggtgggg caggagcggg cgtctcgctc agtccggtcg ggtttttggt cgtagggaac
    33601 aatcaggtaa ggttcctccc ggtgactacg gacgccatat tcgatcgcct catcgatgtt
    33661 gtccccgatc tgattgctcg gcttgcagcg ccgcagccca aaacgattac agtgaggacc
    33721 caggcccaac cagaagtaaa gcgccccata gcatgaagat tgcgtcggca tcccagcgcg
    33781 gactctcagt tgggaatgtg gcggcaggca gtcgacggcc ttttatcgac gcccaatatc
    33841 cacagggcgt gagataaggt taatgccctc tctgcgtgag cctgcttttt gcctgctgta
    33901 gcagacgcat tggggctgtg gtataatccc gactgaatac acgcttccat gtatccgcat
    33961 gcaactggga gattgtgcga gtggggatga tcactgatga tacaacagat cgcggtcatc
    34021 ggattgggca gatttggtac ggcggtggcg cgaaccctgt ccgaccttgg acaggaagtt
    34081 ttggcaatcg atgtggacga ggagaaggtt cagaaattgg cgcctcaagt cactacagct
    34141 gtagtggcaa acgtaacgga catcgatgct atgagggctg tcgggatcag gaacgtagat
    34201 gtggcggtag tggcagtcgg gactgaactg aaagcgagca tcgtaggaac gatggtcatg
    34261 aaggagctgg gcgtaaagtc gatcgtcgca aaagccaacg acgacctcca cgggaaagtc
    34321 ctcgaaaaag tgggggctga ccgggtgatc tatccagagc gggataccgg cgcacgcctt
    34381 gcgcgaatgc ttacttcaac gaatatcatt gagcagatag atttggaccc caattacagc
    34441 atggttgaga tggtggcgcc aagccatatc gcaggccgca cgttggctgc cttgaacatc
    34501 agggcccgtt ttggcgtgta catcatggcg attcgctcgg ccgggcggat gatagtggcc
    34561 cctggagggg acgacgtcat ccacgaaggc gatgttgtgg tggtgatcgg ccaaaatgat
    34621 aaccttcagc gtttcaaaag gctttagaga cagtcatcta gctgccacat ctggatggag
    34681 gcagttctgc ggggaaacca taaggggagc aaacaatgga aagggtccat aagtttctgg
    34741 caagggctgg agtagcgtcg cgccgcaagt gcgaggagct catagccgag ggccgggtca
    34801 gcgtcaacgg agtaacactg aaacaaccag gagttaaggt agatcctgat cgcgacgtgg
    34861 tggcagtgga cggggtgata gtgaggcccc cgtcagaaca tctgtacata gcagtcaaca
    34921 agcccgcagg atttgtgacg acggtccgag atacccatgg gcgccctacc gttcttgacc
    34981 ttgtgcccgg cctcgaccgc agggtctatc cagtgggaag gctggatatg gaatcagagg
    35041 ggttgcttct gctaaccgac gatggcgagc ttgcatacgg gattacccat ccgagccggg
    35101 gagttaccaa aacttaccgc gtaaggcttg cggagccgat cttacaagct cagctagacg
    35161 ttgtagcaag cggcattgaa ttggaagatg gatggactgc gccagctcgg gcgagcatga
    35221 ttgacaactc cggcaggctt gtcgagattg agttgcgtga gggtcgtaaa aggcagatca
    35281 ggcggatgtt tgccgcaatc ggcaatgagg tggttcagct tgtccgcatt gctatcgggt
    35341 gcgtatccct cggatcgctt gctcttgggg caagcagaga gctgacttcc gaagagatca
    35401 gaggtttgag agagcttgcg ggcggtcgtg aacaagcgga attagatgga ggaggatttc
    35461 gcccagatgc aaccggtaag ggcaagagta aggtttgatt acaggggagt tgccgtgccg
    35521 ggacgactgt ttatcgggca taagggcgct gcggaagcat ctgctgcttt gcgcgaccgc
    35581 gccaccgcga acctcaaata cgaggtggag cagggaatca gggtagagga tgtggattca
    35641 ggccacgaaa cgtatgtcct cattgacccc gcaaccgatc aagaagtagc ctattctcct
    35701 gtgtacgttg tcgttcaagc tgactccatc gaagatctcg ccgacttcat actaagggac
    35761 gagtttagaa ccatcgaaat tctcgatcct cctgaattga ctctgtcgcc tcaggaaatc
    35821 ggccgtctcc tcgccagggc taaccaaagg cttaggacaa tggcgcagct tgtggtgaga
    35881 cggagcgaaa gatactgata ctcacgggct tttctgcgtt attcggtatt gcaatgagaa
    35941 ggatttggtc gcttaatgtt taatactgca tttagttgag cgcggtctgt tgctccttac
    36001 ggaggaggat actcaccatg gagctttttg gttcccgtag cggattcact cttgtcgaaa
    36061 ctatgacagc aatagttctt ctatcccttg tcgtcctcat tgtatctgca ggggttcaag
    36121 gctcgctcag tcttagccgg aaagggaaat caaaggctga aggggtagcc atactcaagg
    36181 ctgttaccga agacctgtca acgactccgg caaccttaaa ggatgtggcg gacaaagggg
    36241 agaaaagtta tcctctggag gcattcgact ttgatccatc gtatgaagcc agatactcgg
    36301 tacacggcac agctcatggg gtttatcgcc tcgaggtgag ggtgatacgg gatgggaatt
    36361 gcatcgcgtc caacgaaatg cttatcagga tgcccgacat cggccaatag gcgaggcttt
    36421 tggcgccggc cagaggctgg gatcacactt atcgagctca tgatagcggt tagcatcatg
    36481 gggtttacgc tcatcggaac tctggccttc actcggcttg gcgcctcaac aaagacgatt
    36541 gtcgataggg atgtactcta ttcgcaggag ctgtctttga tgtcagatca gatcatagat
    36601 ggcacgagca ctcggtacag gggtttgcga gaggctgcag acgtcacttc cgataataga
    36661 ctcggctata cgcgctatac ctttcatttc cacggttcgg acgcatcgta tcaggaacat
    36721 tattgggcag tcgatgctgt gctctataga tcaagaggga ctgaaccccc tgagcaaatg
    36781 ggatccgcga actcaattga agtttcgggc cctgatgcca ggggcatgtg tactttgagg
    36841 ctcggattca cagatccgga aggacatgaa gtagaacaca taacgtcagt gcgactcagg
    36901 aatcactcac gttgagcaaa gtcgctgata atgcctgaat atttagtcag gagagggcta
    36961 ttcggcccag ggatgtggag gtgagctatg gtggccttaa gcccgtcaac cgacgaaaga
    37021 ggaagtagcc tggtatatac gctcgtggtt gtggtcgttt tgaccatagc tgttgtcagt
    37081 ctgcttcagc ttgctgctat gtctttcaag acaggcaagt tcgaagagga acgacagcag
    37141 gcgttggctg cggctgagtc ggggatcgcg gtggcgaatg caaggctcgt ttccaacgat
    37201 ccgatgccaa cctcagcata cccttcatct gttccgagca tcgacagcac agcggcacac
    37261 tggattgggc ccggaaccgg tccgattccg aaagagcttc tgctcggtga caacgtgtcg
    37321 tatagggtat gggtttctcc gcctgtagat ggccgtttta ccgtagtctc agaaggcagg
    37381 tgcggcggga ggaccagggt gattcagcaa gcattgtcat catccaccac tgggcttcca
    37441 ctcttgatgt ctgccacttc cacacagcta ttcggcataa tcctaaacgg ctctggcagt
    37501 ctcgatggcg acctgcacac agagggaaag gatgcctggt ccgtagtggc gaacctgtct
    37561 acgccagcca atgggactct ctacgtgagg cccgatgccg atatcaaggg gatacggcgt
    37621 caggtgggga catattttcg ctcagtggag gtcgatccga atacctacag gtatcctgtg
    37681 cccgtctgcc ctgaagggtt ggcacaccgt ggtaagtttc gagccaatgg ttgggagccg
    37741 attgtgataa ccaagagcgg agagtacgac gaattcatag tcaacggaag ttgtgagatg
    37801 atagtggatg cgtctgaatc tgacatcata atccatacga aagaggattt tcttataaac
    37861 ggatccgtca acctgaagat catcggggac catgcgctaa agctatatgt ggacggcaat
    37921 ttcacct
//
LOCUS       DAOSYL010000016        35816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_961_length_35816_cov_8.003216, whole genome shotgun sequence.
ACCESSION   DAOSYL010000016 DAOSYL010000000
VERSION     DAOSYL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 35816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35816
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_35816_cov_8.003216"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLU15_04380"
     CDS             complement(<1..869)
                     /locus_tag="PLU15_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30190.1"
                     /translation="MPEQNALNRAAESADRAQPSLDEKGVARFTNVGALLLRIVVIVT
                     CIMFLLRTLLIQPAIIITIVPAIATVAGFNSIGGKHGYYVASILATLFDLILISATVF
                     ATGGIDSEAFLLYFLEIVVLSLNIGSARCVAAGMGCAGLYSLATWASWANTPETSWIL
                     LYRCGLFMLVGCGVGRLSKAYQASLEMAAMEHERARKRDRWDRTLADLARAINSGLDL
                     HAVLEAVVDGGLDVLEAEAGMIAVVDKQGRTVAEACRALPPDFCGSVLEPGRGAIGLA
                     IESRKTVGILDYQ"
     gene            complement(911..2095)
                     /locus_tag="PLU15_04385"
     CDS             complement(911..2095)
                     /locus_tag="PLU15_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO30191.1"
                     /translation="MNSVLAISLLMLSGVVAGRVVVGWGLSSVVGYLIAGIAIGQIWP
                     SIVEVITPVGVSFAAEVGLALIAFVVGGELEADRVRSLPRGVVGIAFMQAIGCIVAVV
                     TACMWLDQPLGFGLVLGAIAAATAPSTTIMITRKYRSDGPLTNALLSVVAIDDAVCIL
                     LYAIAVAVARALPDGIMSQSPSGVFGVFLWELLGAVLVGVVVGIAMGFFIKYVRGADE
                     LVVVVVGAVLLAAGLASRMHVSPLISSLVLGATVSNLVVGSRRLFASADRFSPPIYVT
                     LFCLAGVGCSFNTLFSSLGLFAAYVVARVAGKVIGAGVASGLSGVGGQIGRHIGLSLL
                     PQAGLAAGLTVAAGVALPDHYQMLASIIVPGMLLFEAVGPPLVAGSLRIAGEIHIGEP
                     SA"
     gene            complement(2123..2494)
                     /locus_tag="PLU15_04390"
     CDS             complement(2123..2494)
                     /locus_tag="PLU15_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30192.1"
                     /translation="MQVLFVVLNDTTLLDDLFSAFVRAGITGATCIESVGMGRTLSEI
                     DNSSIPIFAALRHHLNESRPYNRTIFALLPDDKVDEAISAVESVVGDLSMPGAGVLFT
                     VPVGKVVGFPKRHSSLDELGK"
     gene            3154..3807
                     /locus_tag="PLU15_04395"
     CDS             3154..3807
                     /locus_tag="PLU15_04395"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOO30193.1"
                     /translation="MFKQFIDISRELFTGIEVWPGDPEVQFGYVSSVSSDGAQVMELR
                     LGTHSGTHIDAPAHFIEGAMGIDEVPLSATIGPCLVIDLTNMHGPIDQAALEIHLPEE
                     YPPRLLLKTSHDSWQQAAYLNHEGARFLVESGVKLVGTEEMSIEQTDGDGRVHKTLLE
                     AGVAILEGIDLQGVAPGPYVLLALPLRLKGADGSPCRAVLGVLSEKWQPHVLCNSVP"
     gene            4078..4281
                     /locus_tag="PLU15_04400"
                     /pseudo
     CDS             4078..4281
                     /locus_tag="PLU15_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
     gene            complement(4486..5304)
                     /locus_tag="PLU15_04405"
     CDS             complement(4486..5304)
                     /locus_tag="PLU15_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOO30194.1"
                     /translation="MAYKAPAEIAKTVVDSSVTKANADFVTLVALGFLAGSFIAIAGA
                     TSHTVSLALGPWAGPGIIRFISGLIFTVGITMVIIGGAELFTGNSLMVAGLLEGRITI
                     VQLLRNWVIVYLSNFVGALFVAWLYYKTELWAAPGLEIAEGFVNTAIAKADIEFGTGF
                     ARGILCNWLVCMGVWMSYAADDVTGRVIPTAMAVAAFVMCGAEHSVANMFYMPVGFMI
                     RARISGLPALSMLPNLVSVTLGNVVGGGLLVATVYWVAYLRKSSSPKYVSPGRN"
     gene            5502..6338
                     /locus_tag="PLU15_04410"
     CDS             5502..6338
                     /locus_tag="PLU15_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO30195.1"
                     /translation="MNARTNNNSKHIFTVALLAALILVANAAGASAATVYRVVAGDSL
                     FFIAQRYGVTVSAIRGANGLVGDMIYPGQTLTIPNGVSTTPPAQSGTSYTVKSGDSLF
                     LIARRHGTTVDALKAANGLYSEWIYPGQLLVIPSGTTISPDPNRGGTIPSRGAWSQSD
                     LTLLAQLVTAEAGGEPYEGQVAVAATVLNRISSGLYPNTIPEVIYQVVDGRYYQYSPV
                     LDGRINNAPSSTAIGATREALNGRDPSYGALGFYNPSKTSNYWVRSRTVTRVIGDHVF
                     FK"
     gene            complement(6916..8013)
                     /locus_tag="PLU15_04415"
     CDS             complement(6916..8013)
                     /locus_tag="PLU15_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30196.1"
                     /translation="MPEGEYRSPRHCCAGDQEVGAIAACYGVSVSALLELNPWLSPGE
                     MLAEGDVLVLPAVHGLDGSIAAISDRYGGPGLLIFNAEDWSSKMLASVGCSWASVAAL
                     SPDGRLVAIQGADGKIHIVDVHADLDRAITQPLAGLVTPRWSPSGESIMYEIDAHVYV
                     VDADSGAMKQSFEGQFASWLGDGYRMAYVHSGNVIMREMADGSAVLLTDAPERVCGLA
                     AASYGESVAYIACEDEGVVMCIVDVLTGETVRSKVDREVWPWSIAWAPDGKMIAYCVG
                     QRNLDNGQAVKVIILDPRDGSCRHEAEVVSDPRYSGLAWSPDSRAVAVSARIEGTGRY
                     GVYVVHPGGGLFQLTGTGNVVFPDWGRTAGQ"
     gene            complement(8129..9163)
                     /locus_tag="PLU15_04420"
     CDS             complement(8129..9163)
                     /locus_tag="PLU15_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30197.1"
                     /translation="MGAVNTAQKSNVVEHGEVILQVRDLVKHFPVRMGFWQSMKTKEQ
                     PVLRAVDGVSLDVYKGEILGLVGESGCGKTTLARTILRLTDATSGEAILEGQNIFDLN
                     EAEMKKLRRKMQIIFQDPYESMNPRMDVQTVIAEPLILQGLASSAHDARDVVASALAD
                     VEMVPPEEYMGRYPHELSGGQRQRVAVARALSVDPDFIVADEPVSMLDVSIRGEVLNL
                     MLRLSRSRGVTFIYITHDLATARHICDRIAVMYLGKIVEMGTADEVIQTPLHPYTTAL
                     IGAVFVPDPRHRGFAEVLKGEIPSPVNPPQGCRLHPRCPYTMDICKQVEPPLVLHEGT
                     HQVACHRAER"
     gene            complement(9151..10143)
                     /locus_tag="PLU15_04425"
     CDS             complement(9151..10143)
                     /locus_tag="PLU15_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30198.1"
                     /translation="MALVEVRDLKMYYRTLRGHVKAVDSVSFDIERGKAVGIAGESGC
                     GKSSMAISILRLLPRNGEYHGGSVVMNGQDILKMPEEQFRKDVRWKQISMIFQGAMNA
                     LNPVHRVGDQIVEAILEHEDVSHEEAVSRTKDLFDLVGINPNRFGEYPHEFSGGMKQR
                     AVIAMALACHPDLIIADEPTTALDVMVQAQILKAIGELREELNLSMMLITHDLSVIAQ
                     TCDSVVIMYAGKVAEYGSVMDVYVNPTHPYAMGLVKSFPNIHAERSPVRSLPGFPPNL
                     IAPPTGCRFHPRCPLADEECMTVEPPLREVSRKKHYVACHKAEIVEQGVDIWAQ"
     gene            complement(10146..11813)
                     /locus_tag="PLU15_04430"
     CDS             complement(10146..11813)
                     /locus_tag="PLU15_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30199.1"
                     /translation="MSQQTEPRSKSEEKAAAVKAATVAVPKPLTFWEQYRRKPQGMVG
                     LGIVIVYVLVAIFAPYIAPYDPMEDLYLADRLAAPAWMAKTSAKLKDAPPTIRMNLGP
                     DDWVREEVDDISISPYEFQYFDGVGMEIVLPQTHVVEVEETVEEDTWEEEGGFWASAA
                     IDWDPWGLLESDEETEPSPEPEDEYGFDFEPEIDSGIDASAKKLLLTYEVDYDYTPPQ
                     TFSFSFDYSVDAPSSAETVIMLELMTPDGEVFDVWDAKVYGSVESGTALVDARDYDLK
                     MRLGQGFFDEPTDILFSERGTYTLRLSSWTDSKEGPVTLKVTPVKFEILGLVHGILGV
                     DHMGSDLWSQLIYGTRIALAIGLSSAFIAIVIGTSVGLCAGYFGGAVDEVLMRIADVL
                     MSIPDLPVLIILGALFGKSVLNIVILLAIFSWMGIARLVRSQTLSLKERMFVEAAKAS
                     GASGGYIMREHILPNVMPLIFANLVLRIPGAILTEASLSFLGLGDPRVPTWGRMLQNA
                     KQFGAFTRLAWWWLLPPGIALTLLSLAFVFIGNSVNEIYNPRYRERS"
     gene            complement(11810..12793)
                     /locus_tag="PLU15_04435"
     CDS             complement(11810..12793)
                     /locus_tag="PLU15_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30200.1"
                     /translation="MGFRSYVIKRLVYALIVLYVILTLNFVLFRVMPGDPTKMIIDPN
                     FTPEAKAELRRQYGLDDPPLKQYFTYMGNLLKFDMGLSFSTRRPVWSELKERLPNTIK
                     LFLCTFILTSLTGMRLGVKAAANPGSFTDRVVITAGLFTNAVPTFFIQLLMLLLFGYY
                     WPILPIHGTMSAPPPAGAWNVFLDRLWHLVLPVTSNMIVGFGSWALYARNTMTEALGQ
                     DYIVTARAKGISEKDVLYHHALRSVLPPIVTLLFMSLPGMVSGAVITESIFSWHGVGR
                     YLLDATLQMDYPAAQGAFYLIALAVVISNLLADIAYGLVDPRIRVGAGGAK"
     gene            complement(12883..14589)
                     /locus_tag="PLU15_04440"
     CDS             complement(12883..14589)
                     /locus_tag="PLU15_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30201.1"
                     /translation="MPKKVRLPIAVVLALVLALGIAGAPGAAKMAFETTGPHVDEIIM
                     PIIKEQQARRIAYERGESVVWSGLTQPEDIDRAKAMPNSDMTMTLGFHMFYLCFNMRK
                     EPLASQPIRQAIAHCVDRDNIIRTLFKGYMLPMTSFVPQVSPFYKADVPVYPFSYAKA
                     AEVLDKAGYKLDPVTKVRIDPKTGKPLAQMKIFTPTYEVAATSAELGKMIADSCQKVG
                     LPVVPEPMDFPVMLDKIDIRDFDMYVLAWSLSRNPDFLYDFFHSSQDVEAGYNNPGMR
                     NPEYDKQSELLRYASNLKEAKAASDACQMILAREMPYVPLYSRPYIDAFRKDLVTGYV
                     PMKGFGAASYNNLWTTLNIRRVDRSGRPIEGGTIRWALSEEPKNLNPCVASSAYEQEV
                     LSRLNDSLMAMHPDTLDDMPWMARKWDVGVWEPEAGKKGTTVTWYIEKGIKWSDGMPF
                     TAEDVEFTINYLKANKVPRYLPATQDIAKVELIDKYTVKVYFKNVSYWHIYSADLSFL
                     PKHIWKDVKDWKGFEPWKEPHPKIKGYSKLVGTGPFILKEYVPGEYVRLVKNPNYWRL
                     KK"
     gene            complement(15010..16722)
                     /locus_tag="PLU15_04445"
     CDS             complement(15010..16722)
                     /locus_tag="PLU15_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30202.1"
                     /translation="MPKKVRLPIVLMLAFALALGLAGAPGAAKVAYEPWGPHVDEIIM
                     PIIKESEARRIAFERGESVVWAGLTKPEDIDRAKVIPNADMTMTLGFHMFYLCFNMRE
                     YPLADQAVRQAIAHCTDRDNIIRTLFKGYMLPMTSFIPQVSPFYKADVPVYPFSHAKA
                     AEVLDKAGYKLDPVTKKRIDPKTGKPMRELKIFTPTYEVAPTSAELGKMIADSCQKVG
                     LPVVPEPMDFPVMLDKLDIMEFDMYCLAWNLSRNPTSLYSFFHSSQDVEAGYNRPGIR
                     DPELDKLLEDLNYAPDLKTAKTASDKAQLILAREMPYVPLYSRPFIDAFRKDLVTGYV
                     PMKGYGAASYNNMWTTLNIRRVDRSGRPIEGGTIRWTLDEEPKNLNPCVASSAYEWEV
                     LSRIYDGLQAMDPDTLDDMPWMARKWDVGVWEPEPGKKGTVVTWYINKGIKWSDGQPF
                     TAEDVKFTMDYLKANQVPRYKPTTDDVVKVELIDKYTVKIYYQNVSYWHLYRVAEFLP
                     KHIWQDVKDWKTFEPWKEPHPKIKGYSKLVGHGPYILKEYRPGEYVRLVKNPNYWRLN
                     PPSK"
     gene            complement(17047..17466)
                     /locus_tag="PLU15_04450"
     CDS             complement(17047..17466)
                     /locus_tag="PLU15_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30203.1"
                     /translation="MKMSGGLNPFLIGAIGFAICISSTVGLISIILREYLPVPLWVAL
                     VPAAVAFASLGVASPEVPCMVVLTGTELAIAPLAASLVVGFPASRYQSALVVESAFRL
                     AIAADLSIMIPLAFLSMTAGSAIRGLWIGAGPPPWSG"
     gene            complement(17463..17987)
                     /locus_tag="PLU15_04455"
     CDS             complement(17463..17987)
                     /locus_tag="PLU15_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30204.1"
                     /translation="MGLPHYLRRAEAISGTMAVVAMVASAALIWAIYPLYSDYVRWTE
                     AAAAEVIAASVTLCGDTGTICIDAGFKSPSALFAAEIEALEFNVDCQGRHCGFYRIRV
                     PDDSGILLRDVDNEVLLQLAAPVAPENCGQLSRNGAAVLSGNLIVRIFLPHAEHVARF
                     SLEGELGNVGSDVQ"
     gene            complement(17995..18999)
                     /locus_tag="PLU15_04460"
     CDS             complement(17995..18999)
                     /locus_tag="PLU15_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30205.1"
                     /translation="MALPQPNKESDSIVVVKGLTKHFSVGRGLFRREQARLHAVDDVS
                     FDVARGEVFGLVGESGCGKTTTGRMLVKLTVPTSGDIFVDNEDISAISGQGMREFRQR
                     VQMIFQDPYESLDPRMTVFDIISEPLNVNHIGNLEEREERVAYMLSRVGITPPEMFFF
                     RFPHELSGGQRQRVAIARALVVNPEFIVADEPTSMLDVSIRTGVMKLLAELQEDFGMS
                     YLYITHDLAVARYLCDRIAVMYLGKIVEMAGAEDIIKNPLHPYSKALISAVPLPDPAI
                     RRDVPNIKGGVSKPINPPPRCRFYPRCPVAKNICRDSDHPELCEASPGHFVACHLVAR
                     "
     gene            complement(18980..19984)
                     /locus_tag="PLU15_04465"
     CDS             complement(18980..19984)
                     /locus_tag="PLU15_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30206.1"
                     /translation="MDALLKIENFTMHYRTRAGHVKAVDDVTFSLAHGQSLGLVGESG
                     CGKTSVAMSIMRLLPYNSEIKSGTIVLHGQDIYSMREEEFRKLRWKEVAMIFQAAMNA
                     LNPVYTVGSQITEVILEHEPEVSEREARQRVTELFSTVGIPEERMDHYPHEYSGGMKQ
                     RAVIAMALACNPSLIIADEPTTALDVIVQDKILREMEEIRRKTGMSMMYISHDVSVIA
                     EVSDIVGVMYAGKLVEIGPADDVFKRPRHPYTYGLMNAYPSILGEKRDLATIPGEPPN
                     LMFPPLGCRFHPRCDWATDECGQVEPRARYVGPDHSVACHNPLRAPSSGGEPIGVATA
                     "
     gene            complement(19992..20987)
                     /locus_tag="PLU15_04470"
     CDS             complement(19992..20987)
                     /locus_tag="PLU15_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30207.1"
                     /translation="MMTDPAKNPNIHPSSGMGPTAHSRVIREPLWKIRWRLNMRSLAQ
                     NWAVFKESRIGLAGLAIIVCFGVFGLMWPIMIGNLWDVHIYHPVVGFDPAMMHPAPPC
                     RGHVLGTDPIGRDVLAQLMFSTGREFLLGVLSAVISVVVGTFVGAIAAYYGGLTDSFF
                     MRLADIIMLFPFVPLLVVVGSLVRLDMFSFAIILGFLEAFGGITIILKAQALQVNVRP
                     YIDAAKVAGGDDLHIIRSHIIPNVLPLSFLYMMFNVTAAILSEATLSFFGVLNIDMSW
                     GMMIHTAETAGYLFDYGRYWWLWLPSGLAITLFCAAFYMVGRGLDEVVNPRLRKR"
     gene            complement(20995..22173)
                     /locus_tag="PLU15_04475"
     CDS             complement(20995..22173)
                     /locus_tag="PLU15_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30208.1"
                     /translation="MRRYVLSRVGQLVITLLIYITLVYFLLQAMPGDISMIYLQNPRI
                     PQAAREELKKQLGLDRSPVMQYFSYMKNFFTGNLGVSFSQYPRPVWDILIERLPRTLV
                     LFLTANSLSFYVGFVLGRVIAWKRGASIDHVATVVGITFWTAFYPLLALVLIWLFAYT
                     LKWLPLNQFIEPSLWVHAPWPSNTVFGFMVLGVVLISVSIFIIFWRLTRGQRKTRRNI
                     GLAWGLSAAVVAIALGVAAKAGMLIYIWDIVRHMILPVVTLTLINFAGTMLLMRDSML
                     ETVEEDYVMAARAKGLPDAVVRDRYAARNALLPTVTSLVLSLASSMSGGLVTETMFSW
                     PGLGQVLFRSALANDYPLATGALVFTGIFLLIAHFVADMLYAALDPRISYTGEPAAEG
                     "
     gene            complement(22288..24162)
                     /locus_tag="PLU15_04480"
     CDS             complement(22288..24162)
                     /locus_tag="PLU15_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30209.1"
                     /translation="MKVVRCIITVLLFMVVVFGMGLIGQAAAGGVYEMAIFEDPTTVN
                     VWAALGPDATVWNAFVGYQQSYIGLYGNAAPDFVFCPVAASGMPSPFKEEKIGDKTYY
                     TSVVNIRKELKWSDGRPLTADDVVFSYMAPLALDPNRLGGNWPGNIDPEILAKVEKVG
                     EYSVKFYLTELPGLAQWQYGMLQALILSKAYWDPVVSRALKAADPVKELLAHDPKGEP
                     VAGPWVRDKWEKGAFVTHVPNPYYAMKGDTNTFYKNGAFVTENKKTGFSYVLGDPKGD
                     VKLSVTEGPYANRLMFRILQNQNAAVLSLMAGEVDFVLNSLGLQRGFQDQLKKAANVE
                     VVENNVNGFRYMAFNLSKYPFGIKEFRQAVSVLIDREYICERVLQGVAFPQYSVVPPG
                     NAYWFNSNVPMWGKGMTRSQRITEAVRLLKSAGFSWEVEPQVDLAKDKVLRRGKGLIM
                     PNGKKMQPFEFNVMTAGYDPLRYTFGLNIATWMQEIGIPVEVVPTEFNVVISKTDERD
                     FDCFMLGWSLSLYPDHMYWFFHSSQCPAGGFNYEMYNNPKFDKMVEDFLKESDMETAR
                     KMAFKLQEILADELPYIVLFDTPLIEAYRSDRLAYPYTKTLSGIQYNGGMTSTVRLKN
                     "
     gene            complement(24274..24801)
                     /locus_tag="PLU15_04485"
     CDS             complement(24274..24801)
                     /locus_tag="PLU15_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOO30210.1"
                     /translation="MCNMRRRTMGPAIALMSMLMLLAGHGSTVLAQPSTLVGSRAPEI
                     KAVGVRNESVQVPARGGITVLVFWNSKYRLSLDALAAMQGIWEAYGARGVVCVGLNDL
                     GEEDRAVEQLAERMNIGFVLVSGRTASSAAVAYRLRGVPAVFVVDRSGVIVYVREGWD
                     KKAESEIREIISKAL"
     gene            complement(25048..26100)
                     /locus_tag="PLU15_04490"
     CDS             complement(25048..26100)
                     /locus_tag="PLU15_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO30211.1"
                     /translation="MVDRIAKTQYASELRPGTVVNSVFLCADVKVRPTKSGSQFADVR
                     LVDRTGEISLKLWNYSENVAGALAPGAFVKIRNVPVTEYNGSLQLSCETAQFGGAITL
                     CSSSECNLADYVPSTVRDVDSMIGEIDNAICNIKPGPIKCLLQSFFGMPEFRGEFMEW
                     PAAVRHHHAYSGGLLEHTTGVLRLCLCAADQYEFVDRDILIAGALLHDVGKLRSYDYD
                     RTHGAATMSLPGVMTDHIVIGVEIVNRRIADLLNGCENKGSCAGPCWTEQETLHITHL
                     IASHHGSLEWGSPVQPATIEACILHHADNMDAQVNKYERTIREMLGTGDGQCRYSEHV
                     GKMVWAPPGSFEDDRS"
     gene            complement(26110..26532)
                     /gene="ndk"
                     /locus_tag="PLU15_04495"
     CDS             complement(26110..26532)
                     /gene="ndk"
                     /locus_tag="PLU15_04495"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOO30212.1"
                     /translation="MERTCVLIKPDGIARGLVGEIVSRFERRGCRIVMATTVNMTEEL
                     LAEHYAHLIGKPFFADLKDYMMSGPVIALVLEAPNAVEAVRQIIGATDPIKAGAGTVR
                     GDFATSLHRNLVHASDSLDAAAAEIARFFPRVALQNTK"
     gene            complement(26719..27909)
                     /locus_tag="PLU15_04500"
     CDS             complement(26719..27909)
                     /locus_tag="PLU15_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30213.1"
                     /translation="MKQGLFASGLFLLAAAIVVAYTPAAWAEEALALDAETSTSEGGS
                     SCRTIGVGEFFTAAMSCDPGFLSAWIDTNETRASQAAGQSWTHSTTLWGSDSAIRGSI
                     LATGRSGGRVEFSYDCESSIDSHVQGARIGVEVPLSALADGVLCRRSDSIPTHLHVVE
                     ARLARAEGEAVAGALGCLRRAQIASFMGDMNEAQRAHVELSLRLGCRDGEEIIQALCQ
                     EEFDHVAAMIGSIINSDAGALCEEWIRRDPKVLEAKIDLAAKHESGLAEMLQATSFVA
                     EAKWHALEPNRRCAIGFSLNGRGLARTVGASAEMLEARVEAEQARSTIDFLQVFEAVA
                     EAEAACGFLHGVEGMEADYLEAVLRLEQNGVRLMAGLGMLPYSFLANPHVSPAQRILA
                     GCVK"
     gene            complement(27961..28212)
                     /locus_tag="PLU15_04505"
     CDS             complement(27961..28212)
                     /locus_tag="PLU15_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30214.1"
                     /translation="MDRWLDNIVMAELCPAIIDVKIGVFCFCLAVIFGYPCPSDAELR
                     HHPGCNARFTAAKRHAAASAKPACGCGTGKITLKGDGKT"
     gene            complement(28729..29238)
                     /locus_tag="PLU15_04510"
     CDS             complement(28729..29238)
                     /locus_tag="PLU15_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30215.1"
                     /translation="MRPKSNVRYYWIFAVVVLAGCALASCWGRNGMSEASAARYAYLP
                     DSLNADADVSVAEFAAVRFTAYYNCPGNLTSRLMRQSARCFLGTNSIDLFIDTVTQAY
                     WNTHVAGSRFSVGDDQVIKAYAEAGDVAMDWLRRFFPGVDDRNMRIIFSIKGYQIGVY
                     SCGEFVISR"
     gene            complement(29235..30542)
                     /locus_tag="PLU15_04515"
     CDS             complement(29235..30542)
                     /locus_tag="PLU15_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO30216.1"
                     /translation="MSSQQQSELEFDPKPEDRSVVRHNIRVNNMHGIWESLALNLYSP
                     FLGLLAIRLGASNFQVALITALPAAVSSVASLAGAAFLRLFERKKSAACWLIMANRAF
                     LLAIAALPLIPQEKQAWVFVGLVALMNVPGAISHIAWQSLVADTIPKEYRGDAFAERS
                     RLVTAVGIIPALLGGYLCDSMSFPIGYQLVFVAAFLVSLVEVSVLSKFREPRRVGNPP
                     AAGGGSLDPEAGRPTLAQVLANRGYVSFTLKAMIFYLGWMMSQPLFTIYYVRVLECGS
                     LWVAVFSVVASIARYLSFPKWSRRAAKKGNAWALAAATAGMALTPVMVAMSPWAWLVA
                     VFNVSMGYFTSGTTLLMLNTLLEMVPEKNRTSFIAYHNSAVNLSSFIGPIVGKTLVDS
                     FDIYWALGISAMIRAAGSIGFFLLARTLMSDRRHAGARGGESF"
     gene            complement(30515..31555)
                     /gene="add"
                     /locus_tag="PLU15_04520"
     CDS             complement(30515..31555)
                     /gene="add"
                     /locus_tag="PLU15_04520"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOO30217.1"
                     /translation="MGVSAVGKLPKVELHRHLEGAVRPATALDLFRKNCGMFLDAAVD
                     DILPKMQITEADSSLQDFLAKFDFFMPCLRSAEDITRITVEAIQDCAAEGIIYAELRF
                     SPVFIKGLTGLSYVDAVEAVLEGARIAADCGPIVNFIVIIPQPAGEKAGWDSVRIARD
                     YMADGITGVDIADDTRVLGLEEYVRPMTWARANGLGVTVHAGESEGPESVRTAVERLG
                     ATRIGHGVRAIEDDAVVSLLAEKGILLEICPTSNVHTGIYASVKEHPLPELIKKGVAV
                     CINTDDPKISGITLVDEYELANEVLGLSESEIEDMNFKAIAAAFADDRQRARVRSILE
                     GVANCHRNNNQN"
     gene            complement(31651..32535)
                     /locus_tag="PLU15_04525"
     CDS             complement(31651..32535)
                     /locus_tag="PLU15_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HOO30218.1"
                     /translation="MIRTNRDRLVMVSVQGAVAPHVRKQAFRIDADGVPFVLPGVGGI
                     TYNVRVGDPAFGWAGDHVEPGVSTAADYDKRGDEKNRAYNILACIGNKARVISGDAKG
                     AEGVVTGHHGGIEHVLVDFDDDAMEKLAIDDKILIRAYGQGLELLDYPEIRVFNVDPD
                     LLDLIGLEQDGTRIRVGVAAVVPAVLMGSGIGSADVASGDYDITTTDKAEISRLGIDK
                     LRFGDIVALQDCDNLYGRSYRQGAVSIGVVVHSDCRVAGHGPGVATIMTSAKPTIEPV
                     IDDSANIGRYLNIGRYRK"
     gene            32714..32902
                     /locus_tag="PLU15_04530"
     CDS             32714..32902
                     /locus_tag="PLU15_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOO30219.1"
                     /translation="MSQDSLEWAQAMKLARAYVRPQRYTQRFDPLEGLKNGTIFPELV
                     SPYTPPAETSRSHEEALG"
     gene            32902..33159
                     /locus_tag="PLU15_04535"
     CDS             32902..33159
                     /locus_tag="PLU15_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOO30220.1"
                     /translation="MRTPINREELLLRIQALEFAALDLNLYLNTHPGDARALADFNQI
                     SAALAQTRTAYEQAFGPLMNYGHSLSCGRWAWIDEPWPWEA"
     gene            33194..33766
                     /locus_tag="PLU15_04540"
     CDS             33194..33766
                     /locus_tag="PLU15_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOO30221.1"
                     /translation="MWAYEKKLEYPVKVSGPNPKLAKVVVTQYGGPDGELAASLRYLT
                     QRYTMPTSQAKGVLTDIGTEELAHMEIVAALVYNLLKGAPLSEIKAAGMDGYYADHDR
                     SVYFVNGDGVPWVAAYIQSKGDPIADLHDNMAAEEKARATYEHLINLSDDPQVTDVLR
                     FLREREVVHFQRFGETLNIVQDFLDRRKVW"
     gene            34012..35592
                     /gene="pckA"
                     /locus_tag="PLU15_04545"
     CDS             34012..35592
                     /gene="pckA"
                     /locus_tag="PLU15_04545"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOO30222.1"
                     /translation="MTDIISELRSIGLVNTGLVHHNLSAPQLAEEALARGEGILTSTG
                     ALRVVTGKYTGRSPDDKFIVDDTETHSTVDWGPINKPMSEESFIRLYARLAAYLQRRE
                     LFIFDGYAGTDPLYELPIRVVNEFAWQNLFAHQLFVRPTDDQLKSHKPEFTVIAAPGF
                     KANPEMDGTNSEAFVIINFSKRLVIIGGTSYAGEMKKSIFSVMNYVMPKADVLPMHCS
                     ANIGPDGRSALFFGLSGTGKTTLSADPDRQLIGDDEHCWSKHGVFNIEGGCYAKCINL
                     SREHEPQIWDAIRFGTVLENVVVDPKTRIPDYDDDTITENTRAAYPIYHMPSAAEPSM
                     GDHPSTIIFLTADAFGVMPLISKLDANQAMYYFLSGYTSKLAGTERGIVEPEATFSSC
                     FGAPFMPLPPMVYAAMLGDKLTQNNSSVYMVNTGWIGGQYGVGRRVSLKHTRAMVSAA
                     LSGALDHVSYRAHPIFRLMMPKECPGVPSDILDPKSLWQDAEAYDQAALGLAARFNKN
                     FGKFHDVSPEIASAGPKA"
BASE COUNT         8946 a        10517 c         8494 g         7859 t
ORIGIN      
        1 ttctgataat ccaatatgcc aacagtcttc cgagactcaa tggcaagtcc gattgcaccc
       61 cgccctggtt cgagcacaga gccgcaaaaa tctggaggca gagcgcgaca agcttctgcc
      121 acagttcgac cctgcttgtc aacgacagcg atcatcccag cctcagcttc gagaacatcg
      181 aggccgccat cgaccaccgc ttccaacacg gcatgcaggt ccagccctga attgattgcc
      241 cgggcgagat cagctaaagt gcgatcccag cggtcccgct tgcgtgccct ctcatgctcc
      301 atagccgcca tctccaatga ggcctgatag gccttggaaa gccgccctac gccacagcca
      361 acaagcatga aaaggccaca cctgtataga agaatccagc tggtctcggg cgtgttggcc
      421 catgaagccc atgtggccag cgagtaaagg cccgcacagc ccatccccgc tgctacgcat
      481 cgcgcagaac cgatattgag agacaaaacc acaatctcga gaaagtacag gaggaaagcc
      541 tcgctgtcaa tgccgcctgt tgcaaagaca gtagcggaaa tcagaataag atcgaatagt
      601 gtggcaagaa tacttgccac ataatacccg tgtttgccgc ctatagaatt gaaaccagcg
      661 accgtcgcta ttgcaggtac aatggttatg ataattgctg gttggatcaa cagagttctt
      721 agcaagaaca tgatgcatgt tactatcact acaattctga gcaacagcgc ccctacattc
      781 gtgaatcgcg caaccccttt ttcgtcaagc gagggttgcg cgcggtcagc agattcagcg
      841 gctctattca gagcattctg ttctggcata tgacaccacc actcactaac ccttgaaagc
      901 gctaaacacc ttatgcggaa ggctcaccaa tatggatttc accggcaatc ctcaaagacc
      961 ctgctacaag aggaggaccc acggcctcaa aaagcaacat tcccggtact attatggagg
     1021 caagcatttg atagtgatca gggagagcaa ctcctgcggc aacagtcaag cccgcagcga
     1081 gcccggcctg gggaagcaat gacagcccga tatgccttcc tatctgtccc ccaaccccgg
     1141 acaaccctga agcgacccca gctccgatca cctttcctgc cacgcgggca actacatatg
     1201 cggcgaaaag cccgagtgaa gagaaaagcg tgttgaaact gcatcccacc cccgcaaggc
     1261 aaaacagcgt cacatatatg gggggtgaga acctgtctgc cgacgcaaac aaccgcctgc
     1321 tcccaaccac caggttagat actgttgccc caagaacaag cgaactgatc agtggcgata
     1381 catgcatcct gcttgccagt cccgccgcca gcaacactgc accgaccaca acgaccacga
     1441 gctcgtccgc gcctcttacg tatttaatga agaatcccat cgcaatgcca acaaccacgc
     1501 ctacgagcac cgctccaagc agttcccaca ggaacacgcc gaaaacccca gatggagatt
     1561 gcgacatgat cccgtcagga agcgccctag caaccgcaac cgcgattgcg tagagaagga
     1621 tgcatacagc atcgtcaatg gcaacgaccg aaagcagagc atttgtgaga ggaccgtcgc
     1681 tcctgtattt cctggtgatc attatggtgg ttgaaggtgc agttgctgcc gcgatggcgc
     1741 ctaggaccaa gccaaagcct aatggctgat cgagccacat acatgctgtc accacagcca
     1801 ctatgcagcc aatggcctgc atgaaagcta ttccaactac accgcgagga agcgaacgaa
     1861 ccctgtctgc ctcaagctcg ccaccgacaa caaaagcaat aagggccaac ccaacctcag
     1921 cagcgaatga tactccgaca ggggtgatca cctccacaat gctaggccat atctgcccta
     1981 tggcaatacc ggcgataagg tagcccacca ccgaagatag gccccagccg acaaccaccc
     2041 gcccggccac aacgccgctc aacatgagaa gactaatcgc aagaacgcta ttcatatcaa
     2101 acctctccgc cagctaggga ccttatttcc cgagttcgtc aaggctcgaa tgacgcttag
     2161 gaaatccaac aacctttcca acaggcactg tgaaaaggac tccagcgcca ggcatgctaa
     2221 gatctccgac aacactttcc acagctgaaa ttgcctcgtc caccttatca tcaggcaaca
     2281 gagcaaagat agtgcggtta tatggcctgc tttcgttcag gtggtgccta agggcagcaa
     2341 agataggtat tgatgagttg tcgatttcag acaaagtgcg acccattccc acgctttcta
     2401 tgcaagtagc ccctgttata cctgccctga cgaaagccga aaaaaggtca tccaggagcg
     2461 tcgtatcatt caagacaaca aatagaacct gcaagcaagt tccctccagt aattcctcag
     2521 atagaggcaa gtccattgct tttactataa cctactatgt attgggtgac aagatattgt
     2581 atagtgctcc aatatcagca attacccaaa tcacccttgg attccttcat taagttatca
     2641 tagcccttag cttctccaat atcaactgat gcgtcgtcgg tttatgtcgg aaatacgatc
     2701 tccagagaaa aaccttgtgg gaaaacttgg gagcttgctg ccatactcgc catattccgc
     2761 gccagacaca ccgtttcctc tatgttggtc aatttcgtga gctcgaaaac aagacgagga
     2821 cggccttcac cagcgtccgt gcatcgcaaa aagcggaccg gaaacttccc tgaagcaaaa
     2881 aggctagata cagcttccat aattgtcgcc cccacacgca gaatattctg ggagaaagcg
     2941 cattgatact ccacacaatc tgacgagtct ttcggctatg ggacacactg tacaacaggt
     3001 cagctgtggc acgcgctttt tccaaccttt tcgatatcca ggggcgcgca agagcaaatc
     3061 ttcttgctta atatcagagg gagagacatc aatatgcaga agtaccggtt aacacacata
     3121 tccaaaatgc gatcatcgct tgggggcgat tggatgttca agcaattcat cgatatatct
     3181 agggagcttt tcactggcat agaagtctgg ccgggcgacc cagaggtcca attcggatat
     3241 gtctcatctg tatccagcga tggcgcccaa gtcatggaac tgcgtctggg gacacactca
     3301 ggaactcaca tagacgcccc tgcccacttc atcgaaggag ctatggggat cgatgaagtt
     3361 ccactttctg ctactatcgg gccgtgtctt gtaatcgacc tcacaaacat gcacggaccg
     3421 atcgatcaag ctgctctaga gattcatctg cctgaagagt atcctccgcg actgctcctg
     3481 aagacgtcgc acgattcctg gcaacaagca gcatacttga accacgaggg agccagattc
     3541 ctcgtcgaat ccggagtaaa actggtagga acggaagaga tgtcaatcga gcaaaccgat
     3601 ggagacggtc gtgttcacaa aactctgctg gaagcgggcg tggcaatcct tgaaggaata
     3661 gatctgcaag gagtggcacc cggtccatac gtcctccttg cactacccct gcgtcttaag
     3721 ggagccgacg gatctccatg ccgcgccgtt ctgggggttc tttcagagaa atggcagcca
     3781 catgttttat gcaattcagt tccataattt ttcaaagaca gcaggatgcc caccctggag
     3841 cttagttgga acaaaatagg tttctagtag cgtaggcatc acattgatac aagccgttct
     3901 tactgtagaa tggttacccg cattctggat cttattctct ccgctcgcca acttcgctcg
     3961 agcaaaatgt gagttcctca tgtgacgtaa agttctagta tacgttcgac aacgacggtc
     4021 acactagaaa cgcagtcgca tgcagcaaga atacgcgtga gaagggagga gtattttatg
     4081 gcgagacaga gcagaagcaa taggattctc aacccacagg ctcgtcaggc tcttgaccag
     4141 atgaaatatg agatagccag tcaggtgggg gtcgattata acgcttatgg cgggtatctc
     4201 ggagacattc ccgcccgcga ggccggaagg atcggcggcc agatggttaa gcacatgatc
     4261 gcagcggcgg agcagagcct cattagtcaa accgtcgccg gcactcaggc cagtttcgca
     4321 caggctctga ggccccaagt ttctggcacc ttcccaacag aacccacatc aacctacacg
     4381 actcagacaa accttaacgt gccaaaatca taaaagcttg cccacgcaag cgccggcacg
     4441 taaagacggc gcccggtcgc tctggccgga cgccgtctca ttgcctcagt tccggccagg
     4501 gcttacatat ttaggcgatg agctcttacg aaggtatgcg acccagtaca cagtcgcaac
     4561 caataagcct cctcccacta cgttccctaa tgtcacagaa accagatttg gcaacatgga
     4621 gagggcaggc aatccactga tacgcgcgcg aatcatgaaa cctaccggca tgtagaacat
     4681 gttcgccacc gaatgctcag cgccacacat cacgaaagcg gcaactgcca tcgccgttgg
     4741 tatgactctg ccggtcacat cgtcagcagc ataggacatc caaacaccca tacataccag
     4801 ccaattgcat aggattcccc ttgcaaaccc cgttccaaat tcaatatcgg cctttgctat
     4861 ggctgtgtta acaaagcctt cggcgatttc cagtcccgga gcagcccaaa gctctgtctt
     4921 gtaatagagc catgcaacaa agagggcgcc aacgaaattg ctcaggtaga ctatgaccca
     4981 gtttcggagg agttgaacaa tggtaatgcg cccttccaaa agccctgcaa ccatcagcga
     5041 gttcccggtg aaaagttcag ctccacctat tatgaccatt gttatcccaa ctgtgaatat
     5101 gaggcctgat atgaagcgaa ttattcccgg tccggcccag ggcccgagag ccaaagagac
     5161 agtatgggat gtcgccccag ctatcgctat gaaacttcca gcaagaaatc ccagcgccac
     5221 cagggttaca aaatctgcgt tcgctttggt cactgaagag tcgacaacag tcttcgctat
     5281 ttccgcaggc gccttgtacg ccatttccag caccatcctt tgtccatgaa agtggctgta
     5341 cgccaactat ttcactgaaa tgcaacattg tcaatgtcgc accaagtcgc ttttcccatc
     5401 acttcccaac gggaaattcc cctttctttg acgaatacat cgttagtctg gaagatcgcc
     5461 cccttccaaa ccaggtttat cagagaaagg agttacaact attgaacgcc agaacaaaca
     5521 acaacagcaa acacatcttc acagttgcac ttcttgcagc tctaatcctg gtcgcaaacg
     5581 ctgccggggc atccgccgcc accgtataca gagtggtcgc gggcgattca ctgtttttca
     5641 ttgcccaacg atacggcgta accgtcagtg ccattcgtgg agcaaatgga ttggtaggcg
     5701 acatgatata ccctgggcaa acactgacaa taccaaatgg ggtctcaacc actccgccag
     5761 cacaatcggg cactagttac acagtgaaaa gcggagattc tctgttcctg atagcgcggc
     5821 gacatggaac aacggtagac gccctaaagg ctgccaatgg gctctatagt gaatggatct
     5881 accccgggca actactggta attccttctg gaacaacaat atcgcctgat ccaaatcgcg
     5941 gcgggactat cccttcaagg ggagcctgga gccaatccga cctgactctc cttgcacagt
     6001 tggttaccgc agaagcaggg ggcgaaccct atgaggggca ggttgccgtt gcagccactg
     6061 ttctaaatag aataagcagc ggcctatatc ccaacactat tccagaggtc atctaccagg
     6121 tggtggatgg tcgctattac cagtacagtc ctgtgctgga cggacggatc aataatgcac
     6181 cctcgtccac agctataggc gcaacaagag aggcccttaa cggacgtgac ccatcttatg
     6241 gggctctggg cttttataac ccatccaaga cttctaatta ctgggtgcgt tctcgcaccg
     6301 taacacgggt aataggcgac cacgtattct tcaagtaagg ggcgcaatca tctgtgccat
     6361 acaaacgtaa tggcgcatca attccccgga gcgcgatctc cggggaattg gcttgccacc
     6421 gatgatgaaa gagtccccat cccctcttat gtaagcggct cgagcagcta ttgaattatc
     6481 caacagaaca atgtacaatc gtccgaggga gggacagacc gtgccaatct acgagtacaa
     6541 ctgtgagaag tgtggcaaat tcgaatttct gcatgcagtc ggtgaaggac cccttgcgcg
     6601 gtgtcccaaa tgcggctccc cggtccgtag gcttgtcccc tctagaatgg gcatagcttt
     6661 ccacggatcc ggcttttacg cgaccgatca tcggtcggag gagtacaaaa gagaagcccg
     6721 caaggatgga gagccaggaa cctccgcctc ttaaaacgac taagctaatt caggcatcga
     6781 tcggtagcca cactgatcga ggcgaccgct acgtaatgcg gtcgcctttt aactatccag
     6841 tagccactac acccgtttcc tcggcactct cacggcacgt ctgtgtttct ccaacttcca
     6901 gcctcaccac agatgctatt gtcctgctgt ccttccccag tcagggaaga ctacattccc
     6961 agtccctgtc agctggaaaa gtccacctcc ggggtgaacc acgtacacgc cgtaccgccc
     7021 cgtgccctct attcgagccg acaccgccac cgccctggaa tcagggctcc atgccaaacc
     7081 agagtatctt ggatcgctca caacctccgc ttcgtgcctg cagctgccgt ctcgcggatc
     7141 gagaatgatc acctttacgg cctgaccgtt gtctaaattc cgctggccga cgcaataggc
     7201 gatcatcttc ccatctggag cccaggcaat cgaccaaggc catacttccc ggtcaacttt
     7261 gcttctcact gtttctccgg tcaggacgtc cactatgcac ataacaacac cttcgtcttc
     7321 gcacgcaatg taggccacac tttccccgta agaagcagcc gcaagcccgc aaacccgctc
     7381 cggcgcatcg gttaacaaca cagcgcttcc atccgccatt tctcgcatga ttacattacc
     7441 ggagtgtaca taggccattc tgtagccgtc cccaagccaa gaagcgaact gcccttcgaa
     7501 tgattgcttc attgctcccg aatcggcatc aacgacgtac acgtgcgcat caatctcata
     7561 cattatgctc tcgccactcg gcgaccacct cggggtgacc agtcccgcta ggggctgagt
     7621 aatagcccga tccaagtcgg cgtgcacgtc aacaatatgg atcttaccgt ccgcgccttg
     7681 gatcgccaca aggcggccat caggcgaaag cgcggcaacc gaggcccaag agcacccgac
     7741 cgaagcaagc atctttgatg accagtcttc ggcgttgaag atcaacagcc cagggccgcc
     7801 ataccggtcc gaaatggctg caatagatcc atcaagtcca tggacagcag gaagcactag
     7861 cacgtcgcct tctgcaagca tctctccagg gctcagccaa gggttgagct ccagcaacgc
     7921 agagaccgaa accccgtagc aggccgcaat tgcacctacc tcctggtccc ccgcgcaaca
     7981 gtgtctgggg ctacgatact ctccttcagg cataaaaaac acctcgcttg cgaccgtccc
     8041 tcgaaccaaa tatatgccta ttgcaccaag aaggtgctta gacagacctg gttcgaacag
     8101 tcccaagcga ggcgccttat atccgtaact aacgctctgc cctatggcac gctacctgat
     8161 gagtgccttc atggagcacc aacggaggct caacctgttt acatatgtcc atcgtatacg
     8221 gacatctagg atgcaaccga catccttgtg gcgggttcac ggggctcggt atctcgccct
     8281 tcagaacttc tgcaaacccg cgatgcctcg ggtcgggcac gaacaccgct ccgatcagag
     8341 ccgttgtgta aggatgcaaa ggagtctgga tgacttcatc agccgtcccc atttccacga
     8401 tcttgccgag gtacatcacg gctatcctat cgcatatatg ccgcgcagtg gcaaggtcat
     8461 gggtaatgta gataaacgtg actcctctac tccgcgaaag cctcagcatc aagttgagca
     8521 cttcacccct gatcgatacg tccagcatcg agaccggctc gtctgcgact ataaaatcag
     8581 gatccacgct caacgctcgt gccacagcaa ccctctggcg ctgtccgccg gaaagctcat
     8641 gtggatatct tcccatgtat tcctcgggcg gaaccatctc cacatcagca agggcagatg
     8701 ccacaacgtc gcgtgcatcg tgagccgatg aggcaagccc ctgcagaatc aggggttcgg
     8761 ctataaccgt ctgaacgtcc atacgcgggt tcatcgattc ataagggtcc tggaatatga
     8821 tctgcatctt ccgacgcagc ttcttcatct ccgcctcgtt aagatcgaag atgttctggc
     8881 cttccagaat cgcctcaccg gacgttgcat cagtaagcct caggatcgtt ctggccaggg
     8941 tggttttgcc acaacccgat tcgcccacca agcccaggat ttcgcctttg tacacatcaa
     9001 ggcttacccc gtccaccgcg cgcaataccg gctgttcctt tgtcttcatc gactgccaga
     9061 accccatgcg gacagggaag tgtttgacga gatctcttac ctgcagtatg acctcgccat
     9121 gctcaaccac attcgacttc tgagccgtat ttactgcgcc catatgtcca ccccctgctc
     9181 aacgatttcc gctttgtggc aggcaacata gtgcttctta cgcgaaacct cccgaagggg
     9241 cggttccacg gtcatgcact cctcgtccgc aagcgggcac cgcggatgga acctgcatcc
     9301 agtcggcgga gcaatcaggt tgggagggaa gccaggaagc gatctcaccg gcgacctctc
     9361 cgcatggatg ttggggaatg atttcaccag ccccatggca tatgggtgag ttggattcac
     9421 gtaaacatcc atgacgctgc cgtactccgc aactttgccg gcatacatga taacgacaga
     9481 gtcgcaggtc tgcgcaatca ccgacaggtc gtgagtgatc aacatcatgg agaggttgag
     9541 ttcctctctc agttctccga tagccttcag gatctgcgcc tgaaccatca cgtccagcgc
     9601 cgttgtgggc tcatccgcga taatgaggtc agggtgacat gcaagggcca tggcgatgac
     9661 cgcccgctgc ttcatgcccc cactgaactc gtgcggatac tccccaaatc tgttcgggtt
     9721 aatgccaaca aggtcgaaca ggtcttttgt ccggctcaca gcctcttcat gggatacatc
     9781 ctcatgttcg aggatagcct cgacgatctg atcgccgacc ctgtggactg ggttcagggc
     9841 attcattgcg ccctgaaata tcattgatat ctgcttccat cgaacatctt tgcgaaactg
     9901 ctcctcaggc atcttcagta tatcctgccc gttcataacc acactgccgc cgtgatactc
     9961 accgttcctt ggcaacaggc ggaggattga gatcgccatg ctcgatttgc cacacccgga
    10021 ttccccagcg attcccactg cctttccacg ctcaatgtca aagcttacgg aatcgaccgc
    10081 cttaacatgg cccctgaggg tccggtagta cattttcagg tctctcactt caaccagggc
    10141 cattgttacg atctctccct ataccgtgga ttgtagatct cgttaaccga gttcccgata
    10201 aacacaaacg ccaagcttag aagcgtaagc gctataccgg gcgggagcag ccaccaccaa
    10261 gccagacgcg taaaggcacc aaactgcttg gcattctgca gcattcttcc ccatgtagga
    10321 acgcgcgggt ctcccaggcc aaggaagctc aatgaagcct ccgtgagaat tgctccaggt
    10381 atcctgagga ccagattggc aaagatcagc ggcatcacgt tcggcagtat gtgctcgcgc
    10441 atgatgtaac caccggatgc ccctgatgcc ttagccgcct ctacaaacat ccgttccttc
    10501 agcgacagtg tctgcgatct caccagcctt gcaattccca tccatgagaa gatagcaagc
    10561 aagatgacta tgttaaggac actcttgcca aatagagcgc cgaggataat cagaactggc
    10621 aagtcgggga tggacatgag cacgtccgcg attcgcatga ggacttcatc cactgcgccc
    10681 ccaaaatacc ccgcacaaag gcccacacta gttcctatca cgatcgcgat aaacgcggat
    10741 gacagaccaa ttgcaagcgc aattcgcgtt ccatagatca gctgcgacca gaggtctgaa
    10801 cccatatggt caaccccgag aattccatga accaaaccga gaatctcaaa tttcaccggg
    10861 gtaaccttga gtgtgacagg gccctccttc gagtccgtcc acgagctgag cctcaaggtg
    10921 taagtgcccc tctcagagaa gaggatatca gttggctcgt cgaagaagcc ctggcccagt
    10981 cgcatcttta aatcatagtc tctcgcatca acgagggccg ttcctgactc cacacttccg
    11041 tagacctttg catcccaaac atcgaagacc tctccgtccg gggtcatcag ttccagcatg
    11101 atcacagttt cggcactgct aggcgcatca actgagtaat cgaaactgaa cgaaaatgtc
    11161 tgcggaggag tatagtcata atcaacctca tacgtcaaga gcagcttttt agcggatgca
    11221 tcgatccctg agtcaatctc cggttcgaag tcgaaaccat attcatcctc gggctctggc
    11281 gaaggctcag tctcctcatc gctttccaaa agtccccacg gatcccaatc gatagcagct
    11341 gaggcccaaa agcccccctc ttcttcccac gtatcctcct cgacggtctc ttctacctcc
    11401 accacatgag tctgcggcag gactatctcc attccaaccc cgtcaaagta ttgaaactcg
    11461 tacggggaaa tggatatgtc atccacctct tcgcggaccc aatcatccgg gccgaggttc
    11521 atgcgtatgg tcggcggagc gtccttgagt tttgcgctcg tcttggccat ccaagcgggt
    11581 gcagccagcc tatcagcaag atacaaatct tccatcggat cgtaaggcgc gatatacggt
    11641 gcgaaaatcg caaccagaac gtatacgata actatgccga ggcccaccat accctggggc
    11701 ttgcgcctgt actgttccca aaaggtaagc ggctttggaa cagcgacggt ggcagcctta
    11761 acagcagcag ctttttcctc acttttcgac cttggctcag tctgctgact cactttgcac
    11821 cacctgcccc aacgcggatc ctcggatcaa caagaccgta ggcaatatcc gccagtaaat
    11881 tgcttatcac aacggccaag gctataagat agaaagcgcc ttgcgccgcc ggatagtcca
    11941 tctgaagcgt agcatcgaga aggtaccttc caaccccatg ccatgagaat atgctctccg
    12001 tgataacagc ccctgacacc atcccaggca acgacatgaa gagcaaggta actatcggcg
    12061 ggagaacgct gcgcaacgca tggtgataaa ggacatcttt ctccgaaatc cccttggccc
    12121 gcgcagttac gatgtagtcc tgcccaagcg cttcggtcat tgtgttccta gcatacagag
    12181 cccatgaacc aaatcccaca atcatgttgc tcgttaccgg gagtaccagg tgccatagcc
    12241 tatcaaggaa aacattccat gctccagccg gaggaggcgc gctcatagtg ccgtgtatgg
    12301 gcaatatggg ccaatagtaa ccgaatagga gaagcatgag caactggatg aaaaacgtag
    12361 gcactgcatt cgtaaaaaga cctgctgtta tcacgactcg atccgtaaac gagcctggat
    12421 ttgcagcggc cttcactccc aagcgcatcc ccgtcaatga tgtgaggatg aatgtgcaaa
    12481 ggaacagctt gatcgtattg ggaagccttt ccttgagttc cgaccagact ggccgtctcg
    12541 tcgaaaaaga aaggcccata tcaaatttga gcaaattccc catataggta aagtactgct
    12601 ttaatggggg atcatctaag ccgtactgtc tcctcaattc agccttcgct tccggtgtga
    12661 aattgggatc aatgatcatc ttagtcgggt cacccggcat cacacggaag aggacgaagt
    12721 tgagggtgag tatgacgtat aggactatta aggcatagac caatcgcttg atgacatacg
    12781 aacgaaaacc caaatccgtc tcacctcatt ctgccttatc accggctatg ccgccccagg
    12841 ccccaatggc ccggggcggc aaaaagccag atcatttagg ccctacttct tcagacgcca
    12901 gtagttcggg ttcttaacga gccttacata ttcgccgggc acgtattcct taagaatgaa
    12961 gggtcccgtt ccaacaagtt tggagtaacc cttaatcttc gggtgcggct ccttccaagg
    13021 ctcgaagccc ttccagtcct ttacatcctt ccagatgtgc ttaggcagga aggacaggtc
    13081 tgcactgtag atgtgccagt agctgacgtt tttgaagtaa accttcacgg tatacttgtc
    13141 gatcagctca accttggcaa tgtcctgcgt cgcagggaga tacctcggaa ccttattcgc
    13201 tttgaggtag ttgatggtga attctacatc ctcagcggtg aacggcattc cgtcagacca
    13261 cttgatcccc ttctcgatat accaggtcac cgtggtgccc tttttgcccg cctcaggctc
    13321 ccagacgccg acgtcccact tacgggccat ccaaggcatg tcatcgagag tgtcaggatg
    13381 cattgccatc aggctgtcat tcaaccttga gagcacttcc tgctcgtagg cgcttgacgc
    13441 gacacacggg ttgaggttct tcggctcctc tgaaagcgcc cacctgatgg ttccgccttc
    13501 aatgggcctg cctgaacggt ccaccctacg tatgttcaag gtggtccaca ggttgttgta
    13561 gctagctgcg ccgaagccct tcatgggcac gtatcctgtt accaggtcct tgcggaacgc
    13621 atcgatgtac ggcctcgagt agagcggcac gtacggcatc tctctggcga ggatcatctg
    13681 gcaggcgtct gacgccgctt tagcctcttt caagttagac gcgtacctga gaagctcaga
    13741 ctgtttgtcg tactcaggat tacgcattcc cgggttattg tagccagctt cgacgtcctg
    13801 tgacgaatgg aagaagtcgt acaggaagtc tgggttcctg ctgagcgacc atgccaggac
    13861 atacatgtca aaatcgcgaa tatcgatctt gtcgagcatg actgggaagt ccattggctc
    13921 tggcaccaca ggcagaccaa ccttctggca ggaatcagcg atcatcttgc cgagttccgc
    13981 ggaggtcgcg gctacctcgt aggtaggcgt gaagatcttc atctgggcaa gcggtttgcc
    14041 cgtcttcgga tcgatcctca ccttggtcac aggatccagc ttgtatccgg ccttatccaa
    14101 aacctcagca gctttagcat agctgaatgg atagacagga acatcggcct tatagaaagg
    14161 cgacacctgt ggcacaaaac tcgtcatcgg aagcatatag cccttgaaga gcgtgcgaat
    14221 gatgttatcg cgatcgacac agtgtgcaat cgcctggcgg atcggttgag aagccaacgg
    14281 ctccttcctc atgttaaagc aaaggtagaa catgtggaaa ccaagagtca tcgtcatgtc
    14341 ggagttaggc atggccttag ccctgtcgat atcctcaggc tgagtgaggc cagaccagac
    14401 gacgctttca ccacgctcat atgcgattct tctggcttgc tgctccttta tgatcggcat
    14461 aatgatctcg tcgacgtgcg gaccagtggt ctcgaacgcc atcttcgcgg cgccgggcgc
    14521 gccggcgatg ccgagagcaa gcacgagcgc caagactacg gcaatcggca agcgaacttt
    14581 tttcggcatt aagtacgtac ctccttgaaa tgttaatttg attgcatggg taccagttct
    14641 cgtcactttg cgacatatgc gtcgtgtgat ttgaatcgat ggcccaacaa tccctgcagg
    14701 tgggagaaga caactttgcg gcgataactc tttttagccc atcgccatgc ccaaaaggtt
    14761 gattcgtcac cagatatcag aattccttct accttccatg cgcgatacgg ccaaaacttg
    14821 aacgcacttg aaacaaattt ggttccccgc cttaactgtt gtttattcga ggcttactgc
    14881 taaagtcctt cataccatta ttgatattaa cttcagcaac ctcttacttg cgctagtccc
    14941 gtaccgtaca ggagcggatg ccccccaagg caaacgcctc aggcggcatc cgtcaagaac
    15001 gtcggcgtcc tacttgctcg gagggttgag acgccagtag ttcgggttct ttacgagcct
    15061 cacgtattcg ccaggcctat actccttgag gatgtatgga ccatgtccaa ccagcttaga
    15121 gtaacccttg atctttgggt gaggttcctt ccaaggttcg aatgtcttcc agtcctttac
    15181 atcctgccat atgtgcttcg gcaggaattc ggcgacacga tataggtgcc agtaacttac
    15241 gttttggtag tagatcttaa cagtgtattt gtcgatcagc tcgaccttca caacatcatc
    15301 agtagtgggc ttgtacctcg gcacctggtt tgccttgagg taatccatgg tgaacttgac
    15361 atcctcagcg gtgaacggtt ggccgtcgga ccactttatg cccttattga tgtaccaagt
    15421 tacaactgtg cccttcttac caggctcagg ctcccagact ccaacatccc acttgcgagc
    15481 catccacggc atgtcatcca gggtatccgg atccatcgcc tggagaccgt catagatcct
    15541 ggaaagaacc tcccattcgt aggcgctgct ggcgacgcag gggttgaggt tcttcggctc
    15601 ctcatccaac gtccacctga tagtgccgcc ctcgatcggc ctgccggacc ggtcaacgcg
    15661 gcggatattc agtgttgtcc acatattgtt gtagctcgcg gcgccatagc ccttcatcgg
    15721 aacgtatccg gtaacgaggt ccttcctaaa ggcgtcaatg aaaggcctcg aatacagcgg
    15781 cacgtacggc atctccctgg cgagaataag ctgagccttg tctgatgcag tcttagccgt
    15841 cttgagatct ggggcatagt ttagatcctc aagaagctta tctagctcgg gatcgcgaat
    15901 accaggcctg ttgtagccgg cctcgacgtc ctgcgacgaa tggaagaagc tgtataggct
    15961 tgttgggttc ctgctaaggt tccaggccag gcaatacatg tcgaattcca ttatgtcaag
    16021 tttatcgagc atgactggga agtccatcgg ctcgggcacg acgggaaggc caaccttctg
    16081 gcatgaatcg gcaatcattt tgccaagttc cgcagaggtc ggagcaacct cgtacgtagg
    16141 cgtgaagatc ttcaattcac gcatgggttt gcctgtcttg ggatctattc tctttttagt
    16201 gaccggatca agcttgtaac cggccttgtc aaggacctcg gcagcctttg catggctgaa
    16261 cgggtagaca ggcacgtcag ccttatagaa aggcgacacc tgtggaatga aactcgtcat
    16321 aggaagcata tagcccttga agagcgtgcg gattatgttg tcgcggtcgg tgcagtgtgc
    16381 aatagcctga cgtacagctt ggtcggccaa tgggtattcc ctcatgttga agcaaaggta
    16441 gaacatgtga aaaccaagag tcatagtcat gtcagcattg gggatgacct tcgccctatc
    16501 gatatcctca ggcttggtaa ggccagccca cacaacactt tcgccgcgct caaacgcgat
    16561 cctccgggcc tcggattcct tgataatggg catgataatc tcgtcaacgt gggggcccca
    16621 tggctcgtaa gccaccttgg cggcgccagg cgcgccggct aggccaaggg caagcgcaaa
    16681 tgccaacatc aatacaatcg gcaagcgtac ctttttcggc attgagcatg tacctcctaa
    16741 aaatgattgg tcgtgttact cggtctccaa ttctctctac actacgacgg gatcgtaaag
    16801 ggaatcgaat tgcccgccaa tccctcctct cttgaaccca acaacgattg caaaaacgca
    16861 ctgtcgtcaa ttcttttggc agttaagatg atatccacaa gtttcattcg acaccaagta
    16921 tacgaattcc ttcttcgatt ttactttacg ctcccagcga tcccccacca caattggctt
    16981 gccttccttt tctggctcat gcaaaatcca ttccgctttt caaggactgc catgtgccca
    17041 atggcatcag ccactccacg ggggaggacc agcgcctatc cataacccgc gaatcgccga
    17101 acctgctgtc atcgacaaaa aggctaaggg aatcatgatg gaaagatctg ctgcgattgc
    17161 caagcggaaa gcagactcaa ccactaaggc tgactggtat cgtgacgcag gaaagcccac
    17221 taccagtgaa gcagccaaag gcgctatcgc cagttccgtg cctgtcagca caaccatgca
    17281 tggcacctcg ggcgatgcaa cccccaggga cgcaaacgca accgcagccg gcaccagagc
    17341 aacccagagc ggcaccggca gatactcgcg gagtattatg gatatcaaac ccacggtgga
    17401 ggagatgcat atcgcgaacc ctatagctcc gataagaaac ggattgagcc ctcccgacat
    17461 cttcattgaa catcgctccc cacgttcccc aattcgccct caagagagaa ccgggccaca
    17521 tgttcggcgt gcggcaagaa gatgcggaca atgagatttc cgcttagaac cgcagctccg
    17581 ttccgactca attgtccgca attctccggc gcaacaggcg cagccaattg cagtaatacc
    17641 tcgttgtcga cgtcgcgcag aagaatacct gaatcatcag gcacccgaat cctgtagaat
    17701 ccgcagtgcc tgccttggca gtccacattg aactccaagg cctcgatctc ggccgcaaac
    17761 agggcgctcg gtgatttaaa ccccgcatct atgcatatgg ttcctgtgtc cccacaaagc
    17821 gtgacggagg ctgcaataac ctccgccgct gctgcttctg tccatctcac ataatcactg
    17881 tagagagggt agatcgccca aataagtgct gccgaagcaa ccatggccac tactgccatc
    17941 gtcccggata tggcctcagc ccgccttaga taatgcggca aacccatacc gcagtcacct
    18001 cgccaccaaa tggcaggcca caaaatgtcc aggagacgcc tcacagagct caggatggtc
    18061 actgtcccgg cagatgttct tcgctacagg acaacgcgga tagaacctac acctcggagg
    18121 agggtttatg ggcttactca ctccgccttt gatgttcggg acatcgcgcc gtatcgccgg
    18181 atcaggcagg ggcacagctg aaatcagcgc cttcgaatag gggtgaagcg ggttcttgat
    18241 gatatcctca gctccggcca tctccacaat ctttcccaaa tacatcactg caatcctatc
    18301 gcacaaatat ctggcaacag cgagatcatg agtgatgtat agataactca taccgaaatc
    18361 ctcttgcagt tcagcgagga gcttcatgac tcccgtacgg atcgacacat ccagcatgga
    18421 tgtgggctca tcagctacga tgaattcggg attgaccacg agagcgcgag cgatcgccac
    18481 ccgctgacgc tgaccacccg agagttcatg cgggaacctg aagaaaaaca tctcaggggg
    18541 cgtaatgccc acacgagaca acatgtaggc aaccctctcc tccctttcct ccaagtttcc
    18601 gatgtggttc acattgagtg gttcggatat tatgtcgaat accgtcatcc ttgggtcgag
    18661 cgattcatag gggtcctgga atatcatctg gaccctctgt ctgaattcgc gcattccctg
    18721 gccactgatg gccgaaatgt cctcgttatc cacgaatata tcgccggatg tagggacagt
    18781 aagcttgacc agcatccttc cggtagtcgt cttgccacac ccggattcgc ccaccaagcc
    18841 gaagacttcc ccgcgagcga catcgaaact cacgtcatcc actgcatgga gacgagcctg
    18901 ctctcttctg aacaatcctc ggcccacaga gaagtgtttt gtgagtcctt tgaccactac
    18961 aattgaatca gattccttgt taggctgtgg caacgccaat gggctcacct cccgacgatg
    19021 gagctcgaag tggattatgg catgcaaccg aatggtccgg tcccacgtac cgcgctcgcg
    19081 gttctacctg gccgcattca tcagtcgccc aatcacacct gggatgaaac ctgcatccta
    19141 ggggcgggaa catcaagttg gggggctcgc ctggaatcgt tgccagatcg cgtttttccc
    19201 caagtatgct gggatacgca ttcatcagcc cgtaagtgta cggatgcctc ggacgcttga
    19261 acacatcgtc tgcaggtcca atctccacaa gttttccggc atacatcacg cctacgatgt
    19321 cgcttacttc agctattacc gacacatcgt gagagatgta catcatgctc atgccggtct
    19381 tgcgcctgat ctcctccatc tcgcgcaaaa tcttgtcctg aacaatgaca tcaagcgcag
    19441 tagtgggttc atcagctata atcaagcttg ggttgcaggc taaagccatg gcaatcacag
    19501 cgcgctgctt catgccgccg ctatactcgt gcgggtaatg atccattctc tcttccggaa
    19561 ttccaacagt gctgaacaac tcggttaccc gctgccgagc ctctctttcc gagacttccg
    19621 gttcatgttc gagtatgacc tctgttatct gacttccaac cgtatagaca ggattgaggg
    19681 cattcatggc tgcctggaat atcattgcaa cctctttcca tctgagcttg cggaactctt
    19741 cttcgcgcat cgaatatatg tcttgcccgt gaaggacaat cgtaccgctt ttgatctccg
    19801 aattgtaagg gagcaaccgc attatcgaca tggcaaccga ggttttccca catcccgact
    19861 cgccaacaag gcccagcgac tgcccgtgcg caagcgagaa cgtcacatca tccactgcct
    19921 tcacatgtcc cgcccttgtc cgataatgca ttgtgaagtt ctcgatcttc aaaagagcat
    19981 ccattctcag ctcacctctt tctgagtcgg ggattaacga cctcatcaag gccccggccc
    20041 accatgtaga aagcggcgca gaacagcgtg atcgccagcc cagaagggag ccacagccac
    20101 cagtatcggc cgtagtcaaa caggtacccg gccgtttctg ccgtgtgtat catcatcccc
    20161 cagctcatgt ctatattcag cacgccgaag aaagacagag tagcctcgct cagaatcgca
    20221 gctgtaacgt tgaacatcat atataggaag gaaagcggca ggacattggg tatgatatgt
    20281 gatcgaatta tgtgcaagtc gtctccgccg gcaacctttg ccgcgtctat ataaggcctc
    20341 acattaactt gcaatgcctg agccttgagg atgatagtaa tgccgccaaa cgcctccaag
    20401 aatcccaaaa taatggcgaa tgagaacata tcgagcctga cgagggatcc caccactacc
    20461 aagagaggga caaacggaaa aagcatgatg atatccgcaa gcctcatgaa aaaggaatcc
    20521 gttagccccc cgtagtaggc agcaatagct ccgacaaaag tccctacaac tacactaatt
    20581 actgcggaca atactcccag caagaattcc cggcctgtgc tgaacatgag ctgcgccaat
    20641 acgtcccggc caatggggtc ggttccaaga acatgcccgc ggcaaggcgg ggcgggatgc
    20701 atcatcgcag gatcgaaacc cactaccgga tggtatatgt gcacatccca taagttcccg
    20761 atcattatcg gccacatcag cccgaaaact ccgaagcaaa ctatgatggc caggcccgca
    20821 aggccaatcc ggctttcctt gaaaaccgcc caattctgcg caagcgagcg catattcagc
    20881 ctccacctga tcttccatag cggctccctt atcacccgag aatgcgccgt tggccccata
    20941 cccgagctag gatggatgtt cggattcttt gcaggatcag tcatcattac tcacctaccc
    21001 ttcagccgcc ggttccccag tataggaaat tctcggatcc agagcagcat aaagcatgtc
    21061 tgcaacgaag tgggctatca aaaggaatat gcctgtaaac accaatgccc ctgtggcaag
    21121 cgggtaatca ttggccaaag ccgaacggaa aagaacctga cccagcccgg gccatgagaa
    21181 cattgtctca gtcaccaagc ccccactcat cgaggatgca agcgaaagca ccaagctcgt
    21241 aacggtcgga aggagagcat tcctcgcggc atacctatca cgcaccacgg catcggggag
    21301 ccccttggcc cgggcggcca tcacgtaatc ctcttccaca gtctcaagca ttgagtcacg
    21361 cattagcaac atagtgccag caaagttgat cagagtaaga gtgacaacgg gaagtatcat
    21421 gtgccgcaca atatcccaga tatatataag catgcccgcc ttagcggcaa ccccgagcgc
    21481 aatggcgact actgcggcgg acagtcccca agccaagccg atgttccgtc tcgtcttgcg
    21541 ctgccctcga gtaagacgcc agaatatgat gaatatggac acagagatca agactactcc
    21601 gagaaccata aacccaaaga ctgtgtttga aggccacgga gcatgcaccc ataaactagg
    21661 ttcaatgaac tggttaagcg gcaaccattt caatgtatag gcaaacaacc agatcaggac
    21721 caaagcaagc agcggataga aagcggtcca aaacgttatt cccacaacgg tggccacatg
    21781 gtcgatactg gctccacgct tccaggcaat gaccctgccc aacacgaacc ctacatagaa
    21841 cgacaatgag ttcgcagtaa ggaataacac cagtgtccta ggcaaccgtt cgatgagtat
    21901 atcccagaca ggcctcggat actgtgaaaa cgaaaccccc aaattgcctg tgaagaaatt
    21961 cttcatgtac gagaagtatt gcatgaccgg cgaccgatcc aaacccagct gtttcttgag
    22021 ttcctcccgc gccgcctggg gtattctcgg gttctgaaga tatatcatgg agatgtctcc
    22081 cggcatcgcc tggagcaaga agtagactag tgtaatgtag atgagcaaag tgattaccag
    22141 ctggcccaca cggctaagaa catatctgcg catcttgatc acctatgctt tccggtgttc
    22201 tttgggaaac gaggcgcggg ccgagaggcc tgaggaaggc ctcggcccgc cccgcgcata
    22261 gccaggaacc atatgccatt ccctggacta gttcttcagc ctgaccgtgc tggtcatgcc
    22321 tccgttgtac tgaatgccac ttagggtctt ggtgtagggg tatgcaaggc gatcacttcg
    22381 atatgcttcg atcagaggag tgtcgaatag gacaatatac ggaagctcgt cggccaggat
    22441 ttcctggagc ttgaacgcca tcttgcgagc ggtctccatg tcagactcct tcaggaagtc
    22501 ctctaccatc ttgtcaaact tcggattatt gtacatttca tagttgaagc ctccggccgg
    22561 acactggctg ctgtggaaga accaatacat atgatccgga tagagcgata ggctccaacc
    22621 gagcatgaag cagtcaaagt cacgttcatc agttttggat ataacaacat tgaactcagt
    22681 gggaactacc tccaccggaa tgccaatctc ctgcatccag gtggcaatgt tcaacccgaa
    22741 ggtgtagcga agcgggtcat atcctgcagt catcacgttg aactcaaatg gctgcatctt
    22801 cttgccattc ggcataatca ggcccttgcc gcgtctgagc accttatctt tggcaagatc
    22861 aacctgaggt tccacttccc acgaaaaccc agcgctcttg agcaacctaa cagcctcagt
    22921 tattctctgg ctacgagtca tgcccttccc ccacatcgga acattggagt taaaccagta
    22981 cgcattgcca ggaggcacaa ctgaatactg ggggaatgca acgccctgca gcactctctc
    23041 gcaaatgtac tccctgtcga tcaggactga cacggcctgc cgaaattcct tgatgccgaa
    23101 aggatacttg ctcaaattga acgccatgta tctaaacccg ttaacattat tctccacgac
    23161 ctcgacgttg gccgccttct tcagttgatc ctggaatccc cgctgaagtc cgagggagtt
    23221 aaggacaaag tcgacttcgc cggccatgag ggatagcacc gcagcattct ggttctgcaa
    23281 tatccggaac atcaacctgt tggcgtatgg gccctcggtc acagaaagct tcacgtcgcc
    23341 cttgggatcc cccagcacat agctgaatcc tgtcttcttg ttctccgtca cgaacgcgcc
    23401 atttttgtag aaagtattgg tgtcgccctt catggcgtag taaggattgg gaacatgggt
    23461 aacgaaagct cccttctccc atttgtccct cacccacggc ccggcaacag gctcaccctt
    23521 aggatcatga gccaagagtt ccttcacagg gtcagccgct ttgagcgcgc ggctcactac
    23581 aggatcccag taggccttgg acaaaataag ggcctgcaac attccgtatt gccactgagc
    23641 caagccagga agctctgtca gatagaactt gacactgtac tcgccgacct tctccacctt
    23701 cgcaaggatt tcggggtcaa tattcccagg ccagtttccg cccagcctgt tcggatccag
    23761 agcgagaggc gccatgtagc tgaagacgac gtcatcagcg gttagtggtc tcccatcgct
    23821 ccacttcagc tccttgcgga tgttcacaac tgaggtgtag taggtcttgt cgccaatctt
    23881 ttcctcttta aaaggagatg gcattcccga agcggctaca gggcagaaca cgaagtcagg
    23941 agctgcattt ccgtatagac ctatgtagct ctgctggtac ccgacaaatg cgttccatac
    24001 cgtagcgtcg gggcccaaag cggcccaaac gttcaccgta gtcgggtctt cgaagatggc
    24061 catctcgtag actccgccgg cagccgcctg cccgattaag cccatgccga atacgaccac
    24121 cataaagagc aggaccgtaa ttatgcaccg cacgactttc attgcccaac tgcctccttg
    24181 tcgcggtaat agtctcctgc tcttgtctat tcggccttcg tatggaaatc ccttccgaat
    24241 attatcgtaa ataatccata ttatccatta taattagagg gcttttgata ttatttccct
    24301 gatttccgac tccgccttct tgtcccatcc ctcccttaca tatacgatga cgcccgatct
    24361 gtccaccaca aacactgcag gaacgccgcg gagcctgtaa gcgactgccg ccgacgatgc
    24421 cgtacgccca ctcacaagca cgaagcctat gttcatgcgt tctgcaagtt gctccacagc
    24481 acgatcttcc tcacccaagt cattaaggcc cacacacacg acaccccggg cgccgtacgc
    24541 ctcccatatc ccctgcatgg ccgcgagggc atcaagcgac aatcggtatt tgctgttcca
    24601 gaagacgaga actgtaattc cgccgcgcgc tggcacctga acgctttcat ttctgacccc
    24661 aaccgccttg atttccgggg cccgagaccc tacaagggtg gaaggctgcg ccagaacagt
    24721 tgacccatgg ccagcaagta acatcaacat gctcataaga gcaattgcag gccccatagt
    24781 gcgcctgcgc atgttgcaca ttgcaaatca cctcaaagca acgtcttcct tcaatctaag
    24841 gacatttgtt tacactaagt aagacgcccc aacccgccta gagtttcgaa gcaaacgcgt
    24901 ttttttacag acgttttccc ccataatagc aacccgctac taggttagtc gacaatcaag
    24961 cgcgaagcca tcagcatcca gagattggaa cgcccgtacg cacaaacagg gcccccatcg
    25021 aaatgagagc cctggacgca tcagatctca gcttctatca tcttcaaaag aaccgggagg
    25081 cgcccatacc atcttgccaa catgctcaga atatctgcac tgcccgtcgc ccgttccaag
    25141 catctctctt atagtccttt catacttgtt tacctgggcg tccatattgt cggcatgatg
    25201 aagaatacat gcttctatgg tagccggttg caccggcgat ccccactcca gactcccatg
    25261 atgactcgca atgagatgcg taatgtgtag agtttcctgc tcagtccagc atggcccggc
    25321 acatgacccc ttattctcac acccattgag aagatcagct attcgcctgt tcacaatctc
    25381 aacgcctatg acaatgtggt cagtcatcac gccaggcaga gacatggtgg cggctccatg
    25441 ggtcctgtca tagtcgtaac ttcgcaactt gcccacgtca tggagaaggg cacctgcaat
    25501 tagaatatcg cgatcgacaa actcatactg atccgctgcg cacaagcaca accttaaaac
    25561 cccggtcgtg tgttcgagca gccctcccga atatgcatga tggtgcctga ctgctgcagg
    25621 ccattccatg aactcccccc tgaactcagg catgccaaag aaggactgaa gcaggcattt
    25681 gatgggacca ggtttgatgt tgcatatagc attgtcgatc tccccaatca tcgaatcgac
    25741 atcacgcacc gtagaaggaa catagtcggc cagattgcac tctgatgaac tgcagagagt
    25801 gatcgcccct ccaaactgag ctgtttcaca tgataattgc agggagccat tgtattcggt
    25861 tacaggcaca tttctgatct tcacaaaggc gcctggagcc agcgctcccg caacattctc
    25921 agagtaattc caaagcttca gactaatctc gccagtccgg tccacaaggc gcacgtcggc
    25981 aaactgtgag cccgacttcg ttggcctcac cttcacatcg gcgcagagaa agaccgagtt
    26041 cacaaccgta cccggccgga gctccgatgc atactgcgtt tttgcaatgc gatcaaccat
    26101 ctgaaaacct catttcgtat tctgcagcgc aacacggggg aagaaacgcg cgatctcagc
    26161 ggccgcagcg tcgagactat ccgaagcatg gacaaggtta cggtgtaatg atgtcgcaaa
    26221 atcaccacgc acagtacctg cacccgcctt aatcgggtca gttgctccta ttatctgtct
    26281 gaccgcttcc accgcattcg gcgcttccag cactaacgca atcacagggc ccgacatcat
    26341 atagtctttc aggtcggcaa aaaacggttt tcctatcaga tgggcatagt gctccgccaa
    26401 caactcttcc gtcatgttca cggttgtcgc cattacaatc cgacaacctc tgcgctcaaa
    26461 cctagacact atttcgccca ccaaacccct tgctattcca tctggtttta ttagaacgca
    26521 cgtacgctcc acagcacacc tccatgaaca caccggatct ttcgtctatt gggtaaagtg
    26581 tgcccaattc cgcatcggca cacgccacct gaggtgcaaa gcccgcgtgg ccacgactcg
    26641 ccccctgttg ccgcgcaaat ttgcccaacc gattccatgc tcggtcggct tacacgcatc
    26701 tgccagcata tgtagacgtt atttgacgca accggccaaa atcctctgtg ccggcgacac
    26761 gtgaggattt gcaagaaaag agtacggcag cattcccaat ccggccatga gacgcactcc
    26821 gttctgttca agtctgagca cagcctcgag ataatcggcc tccatcccct ccactccatg
    26881 gaggaatcca caggcagcct cggcctcggc aacagcctcg aacacctgaa ggaagtctat
    26941 ggtgctccgg gcctgttcag cctcgaccct ggcttcgagc atctccgcag aagctccgac
    27001 ggtccgtgca aggccacggc cgtttaggga aaacccaatg gcacatcgtc tgttcggctc
    27061 caatgcatgc cacttggcct ctgctacaaa agatgtagcc tgcagcatct ctgctaagcc
    27121 cgactcgtgt ttggcagcaa gatcaatctt cgcttcgagc acctttggat cgcgccggat
    27181 ccactcctcg caaagcgccc cggcatcaga gttgatgata gagcctatca tcgccgccac
    27241 atggtcgaac tcttcttggc acaacgcctg aataatctcc tccccatcgc ggcaaccaag
    27301 cctcaaagaa agctctacat gagctctctg agcttcattc atgtccccca tgaaagatgc
    27361 aatttgagcc cgcctcaaac aacccagagc gccagccacg gcctcaccct ccgccctagc
    27421 cagcctcgcc tctacaacat gcagatgtgt agggatagag tcagagcgtc tacaaagcac
    27481 cccatcagcc agtgcagaca ggggaacttc aacgccgatt ctcgctccct ggacatgact
    27541 gtcaatgctg ctttcgcaat cataggagaa ctctaccctt ccgcctgacc ttcctgtcgc
    27601 caaaatcgag ccccttattg ctgaatccga tccccacagc gtcgttgaat gtgtccacga
    27661 ttggccagcc gcttgactag cccttgtctc attcgtatct atccacgccg aaagaaaacc
    27721 cggatcacac gacatggccg cggtgaaaaa ctccccaacc cctatggttc gacatgaaga
    27781 accgccctcg ctggtactgg tctccgcatc caaagctaac gcctcttcag cccaggccgc
    27841 aggcgtgtac gcaaccacga tggcagcagc caacaggaag aggcccgaag cgaataggcc
    27901 ttgtttcact tccgaattcc cccttaatgc cattagtata tcaaaggata ggtgaattct
    27961 tcacgttttg ccatcgcctt ttagggtgat tttcccggtc ccacagccgc aggccggctt
    28021 tgctgacgcg gcggcatggc gcttcgcagc ggtaaagcgg gcgttgcatc ctggatgatg
    28081 ccgcaattcg gcgtcagaag gacatggata accgaagatg actgcgaggc aaaagcaaaa
    28141 gactccgatc ttcacatcaa taatggcggg gcatagctcc gccattacaa tattgtctaa
    28201 ccacctatcc acgggcagaa cctgcgccac ctaagtaggt ctcgataagt ttctgtgcgt
    28261 ttctagagct tgcattgcct gcagtcacag ccaagaggct ttatggtttg atggatgcgc
    28321 tgatcgatgc tgtgagcgct tttctcgaca acctgactac gtctgaaatt cagaccttgg
    28381 ccatttaaga atgctcagat gctttttcga agttgctcag gatccagaaa accgaagcca
    28441 aaccgtagcc aaccgaacct gaagtctaga aggccaaatg gaactccgaa cccctaggaa
    28501 ccaaaggaac ccgccgaaaa atccccatcc ccacgagtcg aactgcgtct tccccaaatt
    28561 agcttgcgcc tgcgaacaag gagtatcaac agctcctgcc gtccggaaac cgtagtatcc
    28621 gccacggtga tcaagcagct caatcagaca tattccacag cgcgttgcac tggtatatgc
    28681 ttgatggggc atggtgacgg aacggatatg cagactactc ccattgcatt atctcgatat
    28741 cacgaattcg ccgcagctgt acacccctat ctgatatccc ttgatgctga agattatcct
    28801 catattcctg tcatccacgc cggggaaaaa ccgcctcagc cagtccattg caacatcacc
    28861 ggcttctgcg tatgccttga ttacctgatc atcgccaaca gagaacctgg aaccagctac
    28921 atgagtattc cagtaggcct gtgtaacagt atcaataaac aggtcaatcg agttcgttcc
    28981 taagaaacag cgagcactct gcctcattag ccttgacgtg aggtttccgg ggcaattgta
    29041 gtaagcagtg aatctgactg cagcaaactc ggctaccgat acgtcggcat cggcattgag
    29101 cgagtctggg agatatgcgt accttgcagc actcgcctct gacatcccat tccgtcccca
    29161 acaacttgca agcgcacacc cagcaagtac aaccacagcg aaaatccaat agtatctcac
    29221 attagacttt ggtctcaaaa tgactctcct cctcgcgcgc ctgcatggcg cctatcagac
    29281 atgagtgtcc gggccagcag gaaaaagccg atgcttcccg ctgcccttat catcgcgctg
    29341 atgccaagtg cccagtagat atcgaacgag tcgacaaggg tcttgccaac gatgggtccg
    29401 atgaatgatg aaagattcac tgcactgttg tgataggcaa taaacgaagt cctgttcttc
    29461 tccggaacca tctccaacaa agtattgagc atgaggagcg tggtcccgga tgtaaagtag
    29521 cccatcgaca cattgaagac cgccacaagc cacgcccaag gagacatcgc aaccatcaca
    29581 ggcgtcaagg ccattcccgc cgtggctgcg gcaagcgccc atgcattgcc ctttttcgct
    29641 gcacgcctgc tccacttagg aaatgacaag taccgcgcaa tgctggcaac taccgagaat
    29701 acggcaaccc ataggcttcc gcactcaagc accctaacat agtagatggt aaaaagcggc
    29761 tgcgacatca tccagcccaa gtagaaaatc atcgccttca gcgtaaagct gacatacccc
    29821 ctattggcaa gaacctgtgc aagcgtcggc cggccagcct ctggatccag cgatccgccg
    29881 ccggccgcag gcggattgcc tactcttcta ggctcgcgga acttgctcaa gacactgact
    29941 tccaccaggg aaaccaagaa cgccgccacg aaaaccagtt gatatccgat agggaaagac
    30001 atcgaatcgc atagataccc gccgagaagc gcgggtataa tgccgacagc cgtcaccaaa
    30061 cggcttctct ccgcaaacgc atctcccctg tattctttgg gaatagtatc ggccaccaga
    30121 ctctgccacg ctatgtggct gatggcgcct ggcacgttca tcaatgccac caaacccaca
    30181 aaaacccagg cctgtttttc ctgcgggata agcggaagcg cggcgatagc gagcaagaat
    30241 gccctattgg ccattatcag ccagcatgcc gcagatttct ttctttcaaa gagccggaga
    30301 aatgcggccc cggcaaggct cgctacggaa gacacggccg ccggaagcgc cgttatcaga
    30361 gcaacctgaa aattgctcgc gcccagcctt atcgcaagca acccgaggaa tgggctgtat
    30421 agattgagcg cgaggctttc ccatattccg tgcatgttgt tgaccctgat gttgtgccga
    30481 accacgcttc ggtcttcagg tttcgggtca aactctagtt ctgattgttg ttgcgatgac
    30541 aatttgcgac accctccaga atggaacgca ctcttgcgcg ttgtcgatca tctgcaaaag
    30601 ccgccgcgat tgctttaaag ttcatgtcct ctatttcgga ttcgctcaac cccaaaacct
    30661 cgttggccag ttcatactca tccaccaaag tgatcccgga aattttgggg tcatcagtgt
    30721 ttatacacac tgcaacaccc tttttgatga gttcaggcag cggatgctct ttgacggaag
    30781 cataaatccc ggtgtgcaca ttgctggtgg ggcagatctc cagaagaatc cctttttcag
    30841 ccaaaagtga gacaacagca tcatcctcta tggctctgac gccatgccct atccttgttg
    30901 cccctaatct ctccacagcc gttcgcactg actcgggccc ttctgattct cctgcatgga
    30961 ccgtgacgcc cagtccgtta gctcgcgccc atgtcatagg ccgcacatac tcctcaaggc
    31021 caagcactct tgtgtcatct gcaatatcca cgccggtaat tccatcagcc atgtagtcac
    31081 gggcaatcct gacactatcc cagccagctt tctccccggc aggctgagga attatcacaa
    31141 tgaagttcac aatcgggcca caatccgccg caatgcgcgc tccctccaga acagcctcta
    31201 ccgcatccac atatgaaagt ccggtcagcc ccttgatgaa gacgggcgag aatcgcagtt
    31261 ctgcgtagat tattccttca gcagcgcagt cctgtatggc ctcgacagtt attcgggtta
    31321 tgtcctctgc ggaacgaagg cacggcatga agaagtcgaa tttggccaaa aaatcctgaa
    31381 gagagctgtc ggcctcagtt atctgcatct tgggcaggat atcgtcgact gccgcgtcca
    31441 gaaacatccc gcagttctta cggaaaagat ctagagcagt ggccggccgc acagcgccct
    31501 caagatgtct gtgtagttct accttgggaa gctttcctac agctgaaact cccaccttta
    31561 ccttcccccc cagccaataa atagtagcgg ctcggcgcgc tcactcccga tttccgagtt
    31621 gacatgccgg gccgtctctc ccgagcaacg ttattttctg tatcttccaa tattcagata
    31681 acggccgata ttggccgaat catctatcac cggttcaatt gtgggcttag ccgacgtcat
    31741 aatggtggcc acaccagggc cgtgccctgc cacgcgacaa tcggagtgta ccaccacacc
    31801 aatactcacg gcgccttgcc ggtagctccg tccgtagagg ttatcacaat cctgcagagc
    31861 tacgatatcg ccaaagcgca gcttatcaat accaagccgc gagatctcgg ccttgtcggt
    31921 ggtagtaatg tcgtagtcgc cggacgccac gtccgcagat cctatccccg aacccatcag
    31981 caccgccggc actacagccg caactcccac acggatgcgg gtgccatctt gctcaaggcc
    32041 aatcagatct agcagatcag gatctacatt gaatactctg atttccggat aatcgagcaa
    32101 ctcaaggccc tgcccgtaag ctcggatcag gatcttgtca tctatagcca gtttctccat
    32161 ggcatcatca tcaaagtcca ccaacacgtg ttcgatgcct ccgtgatggc cggttacaac
    32221 cccttccgcc cccttagcgt ccccgcttat cacgcgagcc ttattcccta tgcatgcaag
    32281 gatattataa gcccggttct tttcatctcc ccgcttgtca taatccgcag ctgttgagac
    32341 acctggttca acatggtcac ctgcccaacc gaatgcagga tcacccaccc tgacattgta
    32401 cgtgattcct ccaactccag gaagaacaaa tggaacccca tcggcatcaa tgcggaaggc
    32461 ctgtttcctc acatggggcg ccacagcgcc ctgaaccgaa accattacaa gcctgtctct
    32521 gttagtccta atcatctcta ccaccctcct gtagcatcta acgagatgat tcaccttgta
    32581 tgcggcgatt ccttcacgca ttgattccac cagcacgaac tggatcaaat gtgcgcgtcg
    32641 catcataccc tcggaaaagc tcctgaataa tatggactga gcattcgcat acgcaatcag
    32701 ggaggaaagt aaaatgagcc aagatagtct agaatgggcc caagcgatga aacttgctcg
    32761 cgcctatgtg cgccctcaaa gatacacaca acgcttcgac ccgttagaag gcctcaagaa
    32821 tggcaccatc ttccccgaac tagtgtcgcc ctatacgcct cctgccgaga catcgcgcag
    32881 ccatgaggag gcgttaggat aatgcggacc ccgatcaacc gcgaagaact ccttctgcgt
    32941 attcaagctc tggaattcgc cgcactagat ctcaatcttt acctcaacac ccatcctgga
    33001 gatgccagag cactagcgga cttcaatcag atatccgcag ctcttgccca aacgcgcacg
    33061 gcttacgagc aggctttcgg tccgcttatg aactacggac attcgctatc ttgcggccga
    33121 tgggcatgga tcgacgaacc gtggccgtgg gaagcatagg caagactaga tatgaaacgg
    33181 ggaggatata ggaatgtggg catatgagaa gaaactggaa taccccgtca aggtgagcgg
    33241 gccgaacccc aagctagcca aggttgtggt cacccagtat ggcggccctg atggagagct
    33301 ggccgcttcc ctgcgctatc taacacaacg atacacaatg cccacttctc aggccaaggg
    33361 cgttcttaca gatattggca ctgaagagtt ggcgcatatg gaaatagtcg cagcgctagt
    33421 gtataatctt ttgaagggcg ctccactcag cgagatcaaa gcagccggga tggatggtta
    33481 ttatgcagac catgaccgtt cagtctactt cgtcaatgga gacggagtcc catgggtagc
    33541 cgcttacatc caatccaagg gcgaccccat agctgatctg catgataata tggcagctga
    33601 agaaaaagcc agggcaacct atgaacactt gattaatctg tcagacgatc cacaagtaac
    33661 agatgtcttg cggttcctgc gtgaacgcga ggtagtccat tttcaaagat tcggagagac
    33721 tctcaacatc gtccaggatt tcctcgaccg ccgcaaagtc tggtagtttt gagcaacata
    33781 cgcatggcat gactgtcagt gaagaggcgc gctgcaaaag atcgatatcc tatgttgtgc
    33841 cgccccacgt cgagggcggc ctcttcatag gcggcccact ccaaaccctc tacggcgagc
    33901 accgtccctc aatgggacca accgcagaag gccgacgcgt attcgagtac ttctgacaga
    33961 cgactcggtg tggtaatcta tgaacaactt tagcaacagg aagtgagcat agtgacggac
    34021 atcatctcag aacttcgatc gatcggtctg gtcaatacag gactagttca tcataatctc
    34081 tccgcgccac agcttgccga agaagcactc gctcgcggtg aaggtatact gacgtccact
    34141 ggagcactgc gcgttgttac aggcaagtat acagggcgtt cacctgacga caagttcatc
    34201 gttgacgata cggaaaccca ctccaccgtc gactggggac cgatcaacaa accgatgtca
    34261 gaagagagct tcatcaggct atacgcacgt ctcgccgcct acttgcaaag acgcgagctc
    34321 ttcatatttg acggctatgc aggaaccgat cccctttacg aactgccaat acgcgtggtc
    34381 aacgagttcg catggcaaaa cctctttgcg catcaacttt ttgtacgccc gacagatgat
    34441 caactcaaat cacacaagcc cgagttcacg gtaatagcgg ctccgggatt caaggccaac
    34501 cctgaaatgg acgggacaaa ctcagaagct ttcgtgatca tcaatttctc caagcggcta
    34561 gtgatcattg gaggcacgag ttatgcgggc gaaatgaaga aatccatctt ttctgtcatg
    34621 aactacgtaa tgccaaaggc ggacgtactc ccaatgcact gctctgccaa cattgggccc
    34681 gacggacgga gcgccctctt cttcggcctt tcaggtacgg gaaaaactac cctgtcagcg
    34741 gatcctgaca ggcagttgat aggcgacgac gagcattgtt ggagcaaaca cggagtcttc
    34801 aacatagaag gcggctgcta cgccaagtgc atcaaccttt cgcgggagca tgagccgcag
    34861 atatgggacg caatacggtt tgggactgtc ctggaaaacg tcgttgttga ccccaagact
    34921 cgcattcccg actacgacga cgacacgata actgaaaaca cccgcgcagc atatcccata
    34981 taccacatgc ccagcgcagc cgagccaagc atgggagatc atccttcaac gataatcttc
    35041 ctcacggcag atgctttcgg cgtcatgccc ctaatctcga agctcgacgc caaccaggcc
    35101 atgtattact tcctgtcggg ttacacaagc aagcttgcag gcaccgagcg cggcattgta
    35161 gaaccggaag ccacgttctc ttcatgtttc ggggctccgt tcatgcccct tccacccatg
    35221 gtgtatgccg caatgctcgg cgataagctc acccagaaca actcgagcgt gtacatggta
    35281 aacaccggtt ggataggcgg ccaatacggc gtaggcaggc gcgtaagcct caaacatact
    35341 agggcaatgg tttcggccgc actcagcgga gccttggacc acgtatccta tagagctcat
    35401 ccaatcttca gactgatgat gcccaaggaa tgtcctggag tgccatcaga catactcgac
    35461 cccaagagcc tatggcagga tgcggaagcc tatgaccaag cagcattagg attggcggcg
    35521 cggtttaaca agaactttgg gaaattccat gatgtatcgc cggaaatagc gtctgcgggt
    35581 ccaaaagcgt aacctgagag gttcgtgcag ccatacaaag agcgcctgat cagtcggagg
    35641 atttgcagcg tccgagctgt caggcgttct tttcgcgggg gcggaacaaa atgcggctgc
    35701 aatttctgtt tttgaattct ccacgctcag tctgaaactc ttatgctgaa gcaaccgctg
    35761 tcattatcat catcacatga ccttcttgaa cttccacaac agcatgcttc agaatt
//
LOCUS       DAOSYL010000017        35676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_971_length_35676_cov_7.620673, whole genome shotgun sequence.
ACCESSION   DAOSYL010000017 DAOSYL010000000
VERSION     DAOSYL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 35676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35676
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_35676_cov_7.620673"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLU15_04550"
     CDS             complement(<1..426)
                     /locus_tag="PLU15_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30223.1"
                     /translation="MGVFGWHDKRSSAEKEALQHACTYLYGASQLISYGIALDDNIEV
                     VAEQFPHFGRDNLEKVRESLLKHAEASLRSARLYLECHPDLADFEPNWTPEDTGLIVE
                     FLQCEYGDEQPELSDAMRGDLLAPVYVFLERCVNERRKSS"
     gene            639..1454
                     /locus_tag="PLU15_04555"
     CDS             639..1454
                     /locus_tag="PLU15_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO30224.1"
                     /translation="MVSETLALTWYDAIFDRHSTRNYDGRPVPDGLLDALAPLLSGFA
                     PLCEGARAVIVADSASEVFTGLVGPVGKITAPPVALAFIADTSHPHYQVATGYLGEGI
                     VLEATSLGLATCWVSGSFRRNEAERRVTLAASEKVMAVTPLGFPASSETFGAKAIRTA
                     ASAHKRKPLSSIVSGLPQKDWPPGCGEALEAARLAPSALNRQPWQFNVGEERVTISTS
                     GLDPFPIAPVKLDCGIAMLHFEIAAKANGLRGEWRMSDKAPQVAEFVFTHSSL"
     gene            complement(1710..2822)
                     /locus_tag="PLU15_04560"
     CDS             complement(1710..2822)
                     /locus_tag="PLU15_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO30225.1"
                     /translation="MPPKVYFVSAKDDHGVSLPDKMSRLLDAADIGKGISKGDLVAIK
                     LHVGEPGNLSFVPPQLVRRVVDKVRAVGGNPFLTDANTLYVGQRSNGVNHAMAAISNG
                     FGVEVTGAPFIVADGLTGQDYVGVSMPGPFIREAKIASAIARADAVISVAHFKGHQLS
                     GFGGTLKNLGMGSASRAGKQVQHSDLKPRTNQERCVGCGRCVRYCPAEAIALVDRRAE
                     ADWGKCIGCGECVVVCRHKAMQINWDEGAPGSMQRRMAEYAAAALAGKEGKTGFVNVA
                     IRISPNCDCNPVNDIPVVSDIGLLASHDPVALDQASSDMVVRAAGAPGGACCDVGEHE
                     DKFKSVFPNTDWNTQLEHAEAIGLGSREYELVELMR"
     gene            3592..4497
                     /gene="rsgA"
                     /locus_tag="PLU15_04565"
     CDS             3592..4497
                     /gene="rsgA"
                     /locus_tag="PLU15_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOO30226.1"
                     /translation="MNSDLLQGVVLQASSFFEVETSCGSVFCTARSKAVSPLAQAGGI
                     VPGDRVAITLTPDGRGVIEEVMPRKTQFSRKVAGKRRDEQVIAANLDQVVIVFAAANP
                     PMKETAINRYLTTAEYCGLRSVVCINKADLVSIEVLGEKMSVYSDIGYDVIIASTVSG
                     VGISTLRETLSGKMSALVGPSGVGKSSLISAVEPGLDLAVGDVSGSTGKGRHTTTSGR
                     LIKLAGGGYVADTPGMREFGFYRIPRQELAWCFREFRPHIDACKFWDCTHTTEPGCRL
                     REAVEQGLVSQARYEHYVRLMEEMR"
     gene            complement(4557..5189)
                     /locus_tag="PLU15_04570"
     CDS             complement(4557..5189)
                     /locus_tag="PLU15_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30227.1"
                     /translation="MAQDISIAALLAEEGFNTLAASRSARETLVRNGLTNHRKKNISV
                     QKLSRVREVLARELYRVCDDPWCQAIAGMEARGREIVTVAEDGCQVCRGAKSCRSLKA
                     AALVFERRQIRRVVIVGGTKAGHNEIAAHACGSGVEFRFVDGTKPGRASQLAAEYARW
                     ADLMIVWANTPIKHTVSYTYKGACPKSTRLVYATRTGVAGMLYEVISQIA"
     assembly_gap    5271..5370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5371..5562)
                     /locus_tag="PLU15_04575"
     CDS             complement(<5371..5562)
                     /locus_tag="PLU15_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30228.1"
                     /translation="MRLKILTVLVVEDDHNVRRLLSRYFEAVASRCVNGQLEMDIKWV
                     EEDLLIVETLPRQQAQVDWG"
     gene            complement(5699..6361)
                     /locus_tag="PLU15_04580"
     CDS             complement(5699..6361)
                     /locus_tag="PLU15_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30229.1"
                     /translation="MSGGTHGFPTMQQYFAKRYRVWAIISFVIVLVLSLVAVDSIISK
                     SAARTMLRDLEAGIRKGERGLLGGLLAKDRGGTLNIAPDAVGDYVDTWPDVQRVQDRG
                     KGSGHGNVPWTSGSVVWAARKWHDGTIEVTWTRLLGVRKDATGIYLLVTAAVAVSFWI
                     SAVIAKQGAKRISQMVGSAAEASLKISEGDMELDMEFRATQELSDISDAVNRLASKLR
                     RE"
     gene            complement(6558..6872)
                     /locus_tag="PLU15_04585"
     CDS             complement(6558..6872)
                     /locus_tag="PLU15_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006824501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOO30230.1"
                     /translation="MRKTYDKLVRDRIPEIIEASGKQAVTRIADDSEYEMYLKQKLME
                     EVNEYLASGNPEELADIMEVVAALGQASGIKFEDLVMMAEKKRHSRGGFDSKIVLLHV
                     DD"
     gene            complement(6875..7261)
                     /locus_tag="PLU15_04590"
     CDS             complement(6875..7261)
                     /locus_tag="PLU15_04590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOO30231.1"
                     /translation="MPQCIFCNLPKEKVIAENDLALAFYDAYPVNEGHVLIIPKRHVE
                     TYFDATQDEKNAISLLLAKAKEKLDERFHPDGYNIGVNVGAAAGQTIFHLHVHLIPRY
                     AGDVPDPRGGVRKIKKSLVPYAEEGE"
     gene            complement(7282..9642)
                     /locus_tag="PLU15_04595"
     CDS             complement(7282..9642)
                     /locus_tag="PLU15_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOO30232.1"
                     /translation="MINAITGLDDHLLPRLEAAIGRADRIRLIVAFVMESGVKLILPS
                     LQQAASRGVSIELLTGQYLSITQPSALYLLYDRLGGSIDMRFYNGSIEAFHPKTYIFD
                     HGDEAEVFVGSSNLSRSALTTGIEWNYRFFRSEHPSEYHKFSDTFDELFHKYAQPATA
                     EVLKEYALAWKKPVLIRDVETSTQTVWTGKPEPRDAQIEALYYLKQARDEGASKGVVV
                     AATGVGKTHLAAFDSLEYERVLFLAHRQQILDQAYEVFRSVRPESHFGLYAGEQKDEG
                     ACIYFSTVQTLSRDEHLDVFPKDYFDYIVVDEFHHAAADTYRKVMEHFEPRFLLGLTA
                     TPFRMDNKDIFSLCDDNVVYEISLKQSIERDLLVPFHYYAIYDPTNYDDVRIANGSYV
                     IEDLEKQLSHVERADLVLEHYRKLAGQQCLGFCVSIGHAEYMAEYFSAHGIPSAAVHS
                     GEPGNGYALERDNAIEALEEGRIKVIFAVDMFNEGVDVPSLDTVMFLRPTESYVIFLQ
                     QLGRGLRKYPGKHHLTVIDFIGNYKRAHHIPLLLAGDNPWTHEFSRCLRIEDYEFPEG
                     CTVNFDLRVIDLFDELAARDPLPVRMQDTYWKIKHSLGKRPNRVDIYEGSDIPMREYL
                     RDGWLRFLESVGELEPEEASWLDTPAEAFLVEIEQTRMTKAYKLPTIAAFLRDGDIVA
                     QARLDEIGERMMRFYVDHPVHQKDFRDKSNRKWRQWTADDFAELARKNPVRFLSRGQF
                     FHYDEINRVMYIDSQVQPYLSPHLAEHVQDILSYRRIDYFRRRFKD"
     gene            complement(9704..10564)
                     /locus_tag="PLU15_04600"
     CDS             complement(9704..10564)
                     /locus_tag="PLU15_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO30233.1"
                     /translation="MHVQTVEYKRVDGCSICADVYGSSIPSSPAVVYIHGGGLIWGSR
                     KRVVPEQISAFVGQGYTVISIDHRLAPQTKLPSIVEDVCDALSWVLDDCPRLFGIDPD
                     RVAVFGRSAGAYLALLSGTLARKPKAVVSYFGFGDLLTDWGNKPSGYHLSMPEVSKKR
                     AYECIGSNVISGSGEERLPFYIYCRQQGIWMREVVGLNRDDRVLKEYSPMHNVRSDYP
                     PTLLMHGEEDKDVDCTESVKMANALTHAGVKNELITLPGLGHSFDRQMGDARVRESLE
                     SMLAFLRDNL"
     gene            10985..12286
                     /locus_tag="PLU15_04605"
     CDS             10985..12286
                     /locus_tag="PLU15_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOO30234.1"
                     /translation="MPYVGLAVGTGLLLIAVRHKVNVGLAATIAALVVTAMSGNGIAM
                     LAGALHDTFIDPNGLTLLVSIALITMLGSVMRTSGATDALAQGIRSLVKDDRWVVFLI
                     PSLMGFLSVPGAAVFSAPIIDSVGDALGMSREQKVTANIMFRHAWYYIYPLSTTLLLT
                     QQVSGVPYSAFALPGSIACIIVILSTAKLVFPQEAPPMEPSGTHSRTAFGLDAQSRLP
                     SAVISRARTTFPMILISIAPIIVVLLFALATPVSFPLAVAFSVATGVFVVQPGKPYWQ
                     EVNRRFREGVVRGIDWPVVATVAGTVLLGKTVANSPALDIASASMQTSPASLLLFGIA
                     MPIAAAFVLGSHTAAVAITAPLLLPLIADLPTPFGFVVALFMSSFSGYLVSPAHVCLT
                     ATTQYLGADLGESWRLLIIPSGLSLLFTLAYWGWNALFNLM"
     gene            complement(12395..14059)
                     /locus_tag="PLU15_04610"
     CDS             complement(12395..14059)
                     /locus_tag="PLU15_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO30235.1"
                     /translation="MRLTMLRATCILALAVLLLAVSASGALAAPEAVQDLTGVEEHKP
                     YFIIHLDAISSQVFFEMYDAGLLPNISELFSEGKVIPYAISPFMPGTEMIYPRLKQGA
                     PASSDDPISWTSINRHTGEYKTYLDNVIGLWSWAPRYAFGQLLTGVLGLDALSRLSLA
                     NIERLLRDYGVVEFLWLASDTVGHIKGIDAQKSSAMRLDSMLAPLVRYTKDSPGSLNL
                     VLYCDHGMSMIDEYVLTERAMAEVAGDKALLCFYPNVYLKDERHAPEVARALGERHEV
                     DFAFWRDGEGRIVGCHDKGTVMIDYDDERVAYSCEGSDLFGYVDLGYSGEALTDSEWL
                     DLTARCQWPAVPVQIVRYMQGDYSGDVVVVTNPPKGMPNASGFVARHKGVAASDCTVP
                     VLLVGPDINLDPVPDSIWLQTLYSDVLGIDTVNTLRAEREPHSLSLSSAGVGCTLSPA
                     PSLVLQLELGKGSSDFLCEIDVFRTFNTRIWIGAGIVADRASSLSPCGTFSTRSELFS
                     GRMVFELRKLRFDHKVEQQTSVRYDVGNGITLRWTYPDQFSLGVTW"
     gene            complement(14838..15881)
                     /locus_tag="PLU15_04615"
     CDS             complement(14838..15881)
                     /locus_tag="PLU15_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOO30236.1"
                     /translation="MDARLVRGLIIALIFGTVALLVVFKISGAKVTWSDLMRINPVVV
                     IVALSLVTATWVFDSLRMSALIRAIGEKLGFLSGIRVAITGVFVASLMPFNAGGEPMQ
                     IYLLRNEGLSVGQASAVVAVKTFCNALAKVIVALTASSWIVLSGIGWRVPQQMKAVLL
                     TASSLYVGFFLFSLFLMNDPQLIETVIAPILRSRFVARLLKPDGSERVLKSIRKWISE
                     FQVALAHYWECERWTLLGVIYLSVAGWLALILVPTMILAGLGVRAPSEQVMANVILFH
                     MVSSYVPTPGASGAAELGFAELFRMVVPPDLIGVAVALWRLLTYYFTLLVGGTLTLSR
                     LLRGSVGPAGDEG"
     assembly_gap    16734..16833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17114..17647)
                     /locus_tag="PLU15_04620"
     CDS             complement(17114..17647)
                     /locus_tag="PLU15_04620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO30237.1"
                     /translation="MQENLLPLVSIELENGAVIEAELYPDVAPNTVNNFISLVSKGFY
                     DGLTFHRVIPGFVIQGGCPKGVGTGGPGYSIRGEFEDNGFANDLKHVPGVLSMARAAH
                     PDSAGSQFFIMVGTSPHLDGGYAAFGKVVSGYEEVERIAKVPCDRSDKPLSPVRMKKV
                     TVELFGVEYPEPETVAG"
     gene            18247..19110
                     /locus_tag="PLU15_04625"
     CDS             18247..19110
                     /locus_tag="PLU15_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOO30238.1"
                     /translation="MEEGDVKSLASAMGKRAVNIGMKNDSIKLLRSAMKGRHKSLIAV
                     EGIWLNAKALTARARIETLYVCPTMIHTPEGADLLSRLVDQTMGEICIISENVYHRLS
                     EPKSDEGVISLCRLPEITLNSVRPRTSDLVLVLDGLESPGNVGTIIRTAEAAGACAAV
                     ICHGQANITNPRTVRSSLCSVLSLPVLEADMEDIIRWLRMHEFALYFADASGGVVYSD
                     IEYAPRSALVVGNEKYGINPDWYETEHLSVYIPMMGTVDSLNAGVAASILAYEIRSQF
                     GHTRARSHMKA"
     gene            complement(19188..19970)
                     /gene="csb2"
                     /locus_tag="PLU15_04630"
     CDS             complement(19188..19970)
                     /gene="csb2"
                     /locus_tag="PLU15_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HOO30239.1"
                     /translation="MIGIVGVRCSVEPCFRPPVEETLLVGEMCRRAIMSQYGKAHGGA
                     VSPVLSGKDQSGKVLSGHQHAFYLADDGDGDGLIDTVFVYSVRGFNSLEIDAIARVRE
                     VYLGHYCEPMELRLSGVANMENIAQLGRIFGPSRVWISAVPYVMTRHPKRTRAGETKL
                     TKNGRQQDGPEEQIAREWDQRVHDNCSLPLLIDMQLLGATASRRVYRIVRAYGNAPDP
                     VKRAFKFKLMFDGPLMGPVALGYACHFGLGLFVPETATSPLP"
     gene            complement(19967..20368)
                     /gene="csx15"
                     /locus_tag="PLU15_04635"
     CDS             complement(19967..20368)
                     /gene="csx15"
                     /locus_tag="PLU15_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx15"
                     /protein_id="HOO30240.1"
                     /translation="MIVVNFSHPLSEEQQRRIEALTGQTIDAVYDIPCQLDQDKAFAP
                     QIAGIIDAVPVLAEDWQTNSVLVNLPGLHNAAALVVAELHGRMGHFPACVRIRPVAGS
                     VPTQFEVAEIINLEAVRQEARTRRFPGSANK"
     gene            complement(20388..21614)
                     /locus_tag="PLU15_04640"
     CDS             complement(20388..21614)
                     /locus_tag="PLU15_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1812 family CRISPR-associated protein"
                     /protein_id="HOO30241.1"
                     /translation="MARERVLVFAPGTMPREADYFLPESPDVLETTNLAPIALVKLEK
                     KVRGKEYPDNVIALCTQEVMDERVAGIESDFAKLGIDMSPLEVPNGKDEEELKDIVRI
                     LLQAIPDECDLTLDITHGFRSSPFVFSVAAQYLSFLRPSVTIEGLYYGMLGKAEPNSA
                     PRTPLVDMSIFLELMDWVYAVRVFRDTYMPQKLADMVKRAAGGSDDADGIIESITRFA
                     NAFELGLPIELAERAVDLRKQVSPPLPETLESTVLLPDELFGEVSRLAADFAPASGDR
                     VPDTLSWEEIRRQVRVIDRYIEAGHAVNSVGMMREWVILMVIYYNGDRDKWRTREERK
                     SAEDLANKGRWVSRELYRIKDRVWELRNNLHHHGHEATDNLDVDEMANRVRRVWADLR
                     PYMNRPADWKIEPWGY"
     gene            complement(21621..22736)
                     /gene="cmr6"
                     /locus_tag="PLU15_04645"
     CDS             complement(21621..22736)
                     /gene="cmr6"
                     /locus_tag="PLU15_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HOO30242.1"
                     /translation="MLPLYKDAEEWLQHRQDSNLGLEYDKYIDEWSKTGDAEGVRVSM
                     DPKGKQRFYARFATSPGNYCSSLLRDYRERRYRLLWELGGKVVRARTVWRFVSGLGAS
                     HPLEAGFIWHRTLGVPYLPSSSVKGMVRAWLTHWLGGADDVAAERARARAAELFGDTE
                     TVGMGRLMFFDAVPETPPRLEMDIMNPHYGPYYSDPQKNLPGDYYSPVPVNFLVVPAG
                     QWFEFTLAPADPRCTEDDLAEGIELLQKALEVIGCGAKTAVGYGVFESFQDKTDEVFA
                     EFRRQEENAELEKLSPLERKLRELEIAVEKEGQTDTIIKTTTDLYYEMSTMSDDDKLV
                     VAQTLRDVWMAVGRWSGKLSKKQREKVAEVKRVLGEV"
     gene            complement(22737..23186)
                     /gene="cmr5"
                     /locus_tag="PLU15_04650"
     CDS             complement(22737..23186)
                     /gene="cmr5"
                     /locus_tag="PLU15_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOO30243.1"
                     /translation="MRTLEQERALFAWERVNSVCKDHPNDAKDVAMNIRKMPTMVLTN
                     GLGQTLAFLLQKDEGRRSGPEYIVYDMIAEWVVDKRKLYAGPRADLMNQLIQGDRYLY
                     RQAQEETWAMLAWLKKFADAYIGAGEGLEEKGSDVGGGRPHGGEGRS"
     gene            complement(23186..24142)
                     /gene="cmr4"
                     /locus_tag="PLU15_04655"
     CDS             complement(23186..24142)
                     /gene="cmr4"
                     /locus_tag="PLU15_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOO30244.1"
                     /translation="MDTAKGVLFLYGQSWLRPGAGASTGVIDLPVQREVHTDYPMIPA
                     SSVKGSLRETAEGTVSQDVVDGLFGPEVYRPAAGEPRSKERHFAGALTVGDAKLILFP
                     VRSLTRSFFWVTSPLAIARFLRDLKTVGINPGWDGPCNVDGARALVPQECKTSSRLYL
                     EEMDFEPQKDPAVSAIAQFLAEKFDASIIGAAFNEKLKSDIAVLSDDEFAYFVRYATQ
                     VSARTQLTSNKTTDKTIGPDGKIEQGNLWYEETLPPETVFYIPLMASAAKGGGAQDQL
                     STGNAVMDKLDREVLANHFIQLGGNETLGHGWCAAFLVRGDR"
     gene            complement(24145..25383)
                     /locus_tag="PLU15_04660"
     CDS             complement(24145..25383)
                     /locus_tag="PLU15_04660"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HOO30245.1"
                     /translation="MAEKLFLFGCEESYFFRDGTPFTAGEDTFLPSSFPPSCNVLHGA
                     VRTAIMRGHGLDFGEYAQGRCSVCGCAQEDCEVLKAMSGSPDEPEDAGMDLIGPFVTR
                     ETETGVERLYRAPSDLVQVESVASEIEFGRLHPSPEPVMTDIGRVFMPSSDVRCKSLS
                     DVWISENGLLGYLLGADVSPDEAYYSAKKDGASEQGRAFLLREERIGIARQRATGATQ
                     EGMLYAIEHIRFLHGPTEKFGMGVRVRGCPDTPVPNVVRLGGEGRFTSLRVKRSKPFP
                     ADGVAEAIDRGPSLFGERGFKAVFTTPVQFCAGDDGDGWLPPGFDQCERDGALVWEGI
                     LRDMRCTLVSVCSDRPVRIGGWNMVLNQPKPRLAYVPAGAVYYFTTPHTGAEVVAAFH
                     DAKIGMDTRIGFGHAVVGRW"
     gene            complement(25386..27209)
                     /gene="cas10"
                     /locus_tag="PLU15_04665"
     CDS             complement(25386..27209)
                     /gene="cas10"
                     /locus_tag="PLU15_04665"
                     /inference="COORDINATES: protein motif:HMM:NF023885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOO30246.1"
                     /translation="MEPIGRDKNSVSASCGLGHQPHPERILDFTIAPVQGFVGQARRT
                     RDFWAGSYLISYLTAHAIKAVLQAGPCLPGQTPEQNANRIIFPHIDDDPLLAAVMGRA
                     TPQSGSDEGARIASLPNRFSAECHDPVSAGKAAAQAVQSAWQSLALEVWKTVCNRLRR
                     SFRYYELLPELACQDSLPPTWSRQIENHWELYWVAGAGPSGVDQRKNWRQMHMFDETG
                     EVCTMCGERVIAFGAELPRWQARQIWHGAGGVIESINGESGSGGWALALDPDQKERLC
                     AVCMVKRLFPYIAQEAIGWPVTVSFPSTHDLACRPGEKASSDSGDLAFPYYAILFMDG
                     DSMGKHLSSNPERTGQISRAISGFSRAVPQIVEREYHGKVVYAGGDDVLALLPVDEAL
                     NCAVRLRRMFVESSKLAEVPMTISAAILCVHVKSPLQAALATAHQLLDDVAKEGIGRD
                     AFVIEVRKRGGSPLVVAKPWEDNSRCGAVNRKDGQTWAERIDELSGQIFGSEDEGYSS
                     RFLYSAAELLEPFARSVKEHGVEGPAFEAADMIPVLAAEYLRGPGALLEIDWDESVRR
                     MTELYDLCRWEPRESGKIRDHILQPEALPIVRFLAEAKGVK"
     gene            complement(27212..28303)
                     /gene="cmr1"
                     /locus_tag="PLU15_04670"
     CDS             complement(27212..28303)
                     /gene="cmr1"
                     /locus_tag="PLU15_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HOO30247.1"
                     /translation="MAEPVLEFAQDIDLKVVTPLFLGSATQSKAELRPPSIKGVMRFW
                     HRATGPEGLETEAALFGSSGKAASGQAPFLIRMDTAMLSGQTLDHVKAPLAYLGYGAI
                     NKGQSRPFIPPGTDIKLKLVFKPSASSDVKDGVLRSLRLMNYFGGLGARSRRGWGSVV
                     FADDLPTDFDDLVSRLQDEVAALNIPQSREARYTSFSGESRVVALPAGKSWEEALSTV
                     GQQMMMLRNPKTSPPDYPWAIRDVRMVSGYVKNGAAEQAPYRAMFGLPHNYFFGTTKD
                     KVNIEGANKDFSRRASPLFIHVHLLQNGQYAVVLTFLPAPLLPAGQKISMGTQKQRTI
                     TVSPPTSWGAVEGFLDHIAGQPGAKEVLI"
     repeat_region   29238..30109
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=29238..29273
                     /rpt_unit_seq="gtctccatggttgaaaaaccgtggctccattgaaac"
     gene            30121..30420
                     /locus_tag="PLU15_04675"
     CDS             30121..30420
                     /locus_tag="PLU15_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30248.1"
                     /translation="MIIAMSGLFACKAGKSGSGKNMTHELATARLHRLLQFIRIQRYK
                     VNENPDYPKSVEARTDRLLGSIVLCIIGAATYPPAIATIIIGSAVRNHVENNIAL"
     gene            30493..31206
                     /locus_tag="PLU15_04680"
     CDS             30493..31206
                     /locus_tag="PLU15_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOO30249.1"
                     /translation="MLTSEKSDYTTDSSPTADIVVSDEMLEDFIFCPRLAHLKWAQGE
                     AEPYDSCKDSDKGEPQKTWLMWDAVGVSVGIEKIELSAGESLPVRTHPVTGVETTFIQ
                     ADALILCLQGLALRANSYLCNSGLVHYTDQEKTIHIDFDNALIARTYDSLFELRKTLM
                     ASKTPPPTSDENRCTQCPYAEICMPDEIAFMSTAVSGNLATDEDAVDNAEVRRLVPAR
                     DDRIPLYVQTQGAVVSKRD"
     gene            31519..32094
                     /gene="cas1"
                     /locus_tag="PLU15_04685"
                     /pseudo
     CDS             31519..32094
                     /gene="cas1"
                     /locus_tag="PLU15_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            32099..32389
                     /gene="cas2"
                     /locus_tag="PLU15_04690"
     CDS             32099..32389
                     /gene="cas2"
                     /locus_tag="PLU15_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOO30250.1"
                     /translation="MRRRFIVCYDISDPKRLRKMYRTMRGFGDPLQKSVFTCDLSSKE
                     RAHMVKAIPRIIDCQEDSVLIIDTGGLDAKRESLEYIGKAITPDEGKAAIIV"
     gene            33967..35499
                     /locus_tag="PLU15_04695"
     CDS             33967..35499
                     /locus_tag="PLU15_04695"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOO30251.1"
                     /translation="MPVPNVRSPQDSIAKTAGKVTKRLLTLIVLALLAYHFTGYPAIR
                     RPRPDRTLSPWLAERPIESLLVIAPHPDDETLGCAGVIMYALDQGARVCVALITNGDG
                     SSVPAHTSGKGPLLAPSRYISLGNRRQNETLAALTSLGLEPEDVCFLGYPDGGVSALW
                     SDFWDYSDLFRSQYTKANMSPYSNSFTPNAPYCGLSVVNDICSIIRTFNPSHILLPHP
                     NDTHPDHWGTFALVTYALQRLVCEQELQNPPEMLAYLIHRGDWPVPRLYSPRWPLDPP
                     NTVLRLRAADEWAYQPLTNEDRAQKRQAILEHKSQTAMMRMYMLSFVRSNELFAVLRS
                     PVIAQLPSHQSRSSASRDSHWQTAEPTIWDPQGDTLTRLVSGGADLQAVYAATGNGML
                     YIMAKPKGRPSDNVRYVLDISDFGNPRTQISVSYGPKQSVRTSSSSNLNPDDVQIRRT
                     VDGIEFAIPLTLFQTDRGTGTTAPSAVLLAIHSAMIGDIVVDRTGWTVLRLSEDQPDI
                     FR"
BASE COUNT         8372 a        10487 c         8677 g         7940 t
ORIGIN      
        1 gctgctcttt cggcgctcgt ttacgcaccg ttcaagaaaa acatagactg gagccagcag
       61 atcgccgcgc attgcatcgg atagttcagg ttgctcgtcc ccgtactcgc attggaggaa
      121 ctccactata agtccggtat cttccggagt ccaattaggc tcgaaatccg caagatccgg
      181 atggcattcg aggtacagtc gagccgatct cagactggct tcagcatgtt tcaacagaga
      241 ctcacgcacc ttctccaagt tatcgcggcc aaagtgcggg aactgttcgg cgactacctc
      301 aatgttgtcg tccagcgcaa ttccgtaaga aatcaactgg cttgccccgt acagataagt
      361 gcaggcatgc tgcaacgcct ctttttccgc agacgaacgc ttatcatgcc atccaaatac
      421 gcccatcctt gctcccccct gtcataagac tatacctttc ttgcttagcc atcaaagcgc
      481 gtttcccttc aaacggctgt atggcctcgg tagtgcatac agcttctgcc gcgcacgtcc
      541 agcacatatt cttacacagt cggttgcagg aatcgcttag cctcaagttg aacaaggacg
      601 cagacgaacg ttagtgtgga aagcaaagga ggaagggaat ggtttctgaa actctggcat
      661 tgacatggta tgacgcgata ttcgatcgcc attcgactag gaactacgac ggcaggcccg
      721 ttcccgacgg cctgcttgac gcgcttgcgc cccttctttc cggtttcgca ccgctatgtg
      781 aaggggcccg cgctgttatc gtcgcagaca gcgcatctga agttttcaca ggccttgtcg
      841 gccctgtggg caaaatcaca gctccccccg tcgcgctggc gttcatcgca gacacctccc
      901 atccccatta tcaagtggcc acgggctact tgggtgaagg aatcgtcctt gaggctactt
      961 cactgggcct tgctacctgc tgggtcagcg ggagtttcag gcgcaacgaa gcagaacgca
     1021 gggtaaccct tgcagcatca gaaaaagtca tggccgtgac gccactgggc tttcccgcgt
     1081 ctagcgagac tttcggcgcg aaggcaataa ggaccgcggc ttctgcgcat aagagaaagc
     1141 ccctgtcatc gattgtcagc ggactgcctc agaaagactg gccacccggc tgcggtgaag
     1201 ctcttgaggc ggccaggctt gcgccgtcag cgctgaacag gcaaccatgg cagttcaatg
     1261 taggcgaaga gcgggtgacg atatccacgt ccggacttga tccgtttcct atcgccccgg
     1321 tcaagctgga ctgcgggatc gcaatgctcc atttcgaaat agcggcgaag gccaacggtc
     1381 tccgcggaga atggaggatg tccgataaag cgccccaggt ggcagagttc gttttcacac
     1441 actccagtct gtagatccca tacacatacg agaaccgagg gttttgtggg aggtgttttg
     1501 atggcctgaa ggcttcagcc gggatgcaca cgctctcagg cccgcctccc acagatctcc
     1561 cttcgttatt catataacag gcaggcccat atcaaagctt ttctggcgtg aagacgaatg
     1621 ccacgtaggc cactgcaata gctgcgagcc tcaatgcccg ttggccgggc gccatcacca
     1681 gccgcatgtc tgcctcggca ggacccttgc taacgcatta gttcaacaag ttcgtattct
     1741 ctgcttccaa ggcctatagc ctcagcgtgc tccagctggg tgttccagtc ggtattggga
     1801 aagacggatt tgaacttgtc ttcatgttcg cccacgtcac agcatgcccc gcccggggct
     1861 ccggctgccc gaaccaccat atccgaactg gcttgatcga gtgcaacagg gtcatgggat
     1921 gcgagcaatc ctatgtccga gactactgga atgtcattga ccgggttgca gtcacagtta
     1981 ggactgatcc tgattgcgac gttcacaaaa cccgtcttcc cttccttgcc ggcgagcgcg
     2041 gccgcggcgt attcggccat cctgcgctgc atgcttccgg gcgccccttc atcccagttt
     2101 atctgcatag ccttgtgtct gcacacaacc acgcactctc cgcagccgat gcactttccc
     2161 cagtctgcct cagcacgcct gtcaacgagt gctattgcct cggcaggaca atatcgcaca
     2221 catcgtccgc acccgacgca cctttcctga ttcgtcctgg gcttcaagtc ggaatgttga
     2281 acctgtttgc ccgccctgct ggcgctcccc atgccaagat tcttcagtgt tccgccaaac
     2341 cctgacagct ggtgcccttt gaaatgggca acggatatta cggcatctgc gcgggctatt
     2401 gcggacgcga tctttgcctc acgtatgaag gggcccggca tggaaacgcc aacatagtct
     2461 tgcccagtga ggccatcggc cacgatgaac ggtgcgcccg taacctccac gccaaagccg
     2521 ttggatattg cagccatggc atggttcact ccgttcgagc gttgacctac gtagagagta
     2581 ttggcatctg ttagaaacgg gttgcctccc actgctctca ctttatcgac taccctccgc
     2641 actagctgcg gcggcacgaa tgataggttt ccgggctccc ctacatgcag cttaattgca
     2701 acaaggtcgc ccttggatat acctttgcct atatcggcgg cgtcgagcag ccgcgacatc
     2761 ttgtcgggca aactcacgcc gtggtcatcc tttgcactga cgaagtacac cttcggtggc
     2821 atcaagtctc ctcctcgcta acacgcgttg cttgctgatg ctattttgac atgcacatgc
     2881 cattcacctc catccacccc gcatccaggc gcacgccgat tgctgcccaa ggctgagcac
     2941 cagaagccct gctatcatgt gcaggcactg tcgagccgtt tgcgtgcttg gtatgaatgg
     3001 cagcaagggc cggtgcttcc tgtcggagat aggctgggaa aacggagtct aaccccacga
     3061 gccgtcgtcc ctcgcacgcc ccaaattctc cggcacaacc caacctgtgc agtgtttgac
     3121 atcgaaacgg ccaaatgata gcatatggtg cagatatgag cggtacggtt cggtagatcc
     3181 ggcccggcgg aagaggtggt ttttatgtcc tacatgatga ttgagcgttc ttcgcacgaa
     3241 tattgtccaa cccaccgttc gccggttgtc gcacaggccg atctgaacag gtagttgccg
     3301 ttggacggca tgggcaagcg cttgtctgct caagctcggc ttggcccgca aagcattcgt
     3361 tcacatgcag gctgtatgcc atctgcggtc catgcgtaga ggctttgccc tgtcctgatg
     3421 atcgattcta cacgagcgtg atatgcgcgc ctgcggaagc gggcgcgctt tgttgttctg
     3481 ccgcttcagg ctgctgcgga gcaagccgga ttgcgtccgt ttcctccatg gcgacaacgg
     3541 gacgttgggc acgtcgacga tccaccacac gcacaggagg catcggacga attgaacagt
     3601 gatctattgc agggcgttgt tcttcaagca tcttctttct ttgaagtaga gacatcatgt
     3661 ggcagcgtct tctgcacggc acggtccaag gcggtatctc cgctggcaca agcaggcggg
     3721 atcgtgcctg gagatagggt cgcgataacg ctcacgccgg acggcagggg cgttatcgag
     3781 gaagtgatgc cgcgcaaaac tcagttttcg cggaaagtgg caggcaagcg ccgagatgaa
     3841 caggttatcg ccgcgaactt ggatcaggtg gtcatagtgt tcgccgcggc caacccgccc
     3901 atgaaagaga ccgccataaa cagatatcta actacagcgg aatactgcgg cctcagatcc
     3961 gtcgtatgca tcaataaggc ggacctggta tccatagagg ttctcgggga aaagatgtcg
     4021 gtctacagcg atatcgggta tgacgtcatc atagcatcta ccgtgtcggg tgtcggcatc
     4081 agcacgctgc gcgagactct ttccggcaag atgtcggctc ttgtcgggcc gtccggggta
     4141 ggaaagtcgt cgctcatcag cgccgtcgag ccgggcctgg atcttgcagt aggtgatgta
     4201 agcggaagca ccggaaaagg cagacatacg acaacttctg gacggctgat caagctggca
     4261 ggcggcggat acgtggcgga caccccaggg atgagggaat tcgggttcta ccgtatccca
     4321 cgccaagaac tagcatggtg tttcagggag ttccggccgc acatagatgc gtgcaagttc
     4381 tgggactgca cccacacaac agaaccaggg tgccgcctcc gagaagccgt agaacaaggc
     4441 ctagtttcac aggcacgata tgaacattat gtgagactta tggaagagat gcggtagggc
     4501 attcgccagc cctctgcgca ggttgtggag agtacgaccc cgcagagggc tgtttttcaa
     4561 gctatctggc tgatgacttc atatagcatt cctgccaccc cggtccgcgt ggcatataca
     4621 agccgggtgg actttggaca cgcaccttta tatgtgtagc tgacagtgtg tttgatcggg
     4681 gtgttggccc agactatcat caaatcggcc cagcgggcgt attctgcggc aagctggctg
     4741 gcccggccgg gtttggttcc gtcgacgaac cggaattcta caccgctacc acaagcgtgg
     4801 gcagctatct cgttgtggcc ggccttcgtg ccgcccacga taacgacccg gcggatctgc
     4861 cgcctttcaa agaccagcgc tgctgccttt agcgacctgc atgattttgc gcccctgcac
     4921 acttggcacc cgtcttctgc aacagtgacg atttcccgcc cacgagcctc catgcctgca
     4981 atcgcctggc accatggatc gtcgcacaca cgatacagtt cccttgcaag aacctcacgg
     5041 acccgggaca gcttctgcac gcttatgttc ttcttccggt ggtttgtgag gccgttgcgc
     5101 acgagcgtct cccgagcaga tcgactggca gctaaagtgt taaacccctc ctccgccaac
     5161 aaagcagcga tggaaatatc ctgtgccatc tgcgtcagcc tccctccgta gttccggtaa
     5221 gacaaggata acagatagcc ccggcacaga cttgtcagtt acgtccgata nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccccaatct acctgagcct gttgtctagg
     5401 aagggtctct actatcaata agtcttcttc cacccacttt atgtccattt caagttggcc
     5461 attaacacat ctactcgcaa ccgcctcaaa ataccgggaa agcaggcgtc ggacgttgtg
     5521 gtcgtcttct actacaagga cagtcagtat cttcaagcgc aatacggtgt gcctccttca
     5581 ggtacccggt tgaggtggac aacgagtcgt tttgaaacaa ggcagcacat tctgcgctgg
     5641 cgacaccagc atttaatcca tgcattcgag taaggtgagg ccgcgtctca agctgcatct
     5701 actctcttcg aagctttgaa gcaagcctgt tgacggcgtc tgatatgtct gaaagctcct
     5761 gggtcgcccg gaactccata tcgagctcca tatcgccctc tgatatcttc aagctcgcct
     5821 cggcagctga ccccaccatc tgggatattc tctttgcacc ctgcttggct atgacggcac
     5881 tgatccagaa agaaaccgcc actgcagcgg taacaagtag atagatgcca gttgcatcct
     5941 tcctaacgcc gagcaggcgg gtccacgtga cctcaattgt gccgtcatgc cattttcggg
     6001 ccgcccagac cactgacccg ctggtccaag ggacatttcc gtggccggat ccctttcccc
     6061 gatcctgcac ccgctgaaca tccggccatg tgtcaacata gtctccaact gcatcagggg
     6121 ctatattcaa ggttccaccc cggtccttgg caagcaagcc gcccaacagg cccctctctc
     6181 ctttccggat gccggcctcc agatcccgaa gcatagtgcg ggctgctgat ttcgatatga
     6241 tagaatcaac agcaacgagc gagagtacta gaacaatgac gaaacttata attgcccaaa
     6301 cgcgatacct tttcgcaaaa tactgttgca tggtaggaaa cccgtgtgtt cctccgctca
     6361 tgaaaatcca ctcctttgca tgatcccaca ttctggccta gccaagcccg tgtcgcagaa
     6421 gtaagactgt caatcagttc ttctttggat aatccatcca taccgcatct gtactgataa
     6481 atagcacaaa ggccaacaga tatcctccat tggccggcga caaatataca attgttgcag
     6541 tttaaatgca caacaggcta atcgtcgacg tgaagcagca caatcttgga atcaaagcca
     6601 cctcgggagt gtctcttctt ctcagccatc attactagat cctcgaactt aattccagac
     6661 gcctgcccca gagccgcgac aacttccatt atatctgcaa gttcttcagg atttccactg
     6721 gccagatact cattgacttc ctccatcagt ttctgcttga gatacatctc atactcacta
     6781 tcatcggcga ttcgagtaac cgcctgtttc cctgacgctt cgatgatctc tggtattcta
     6841 tctctaacca gcttatcgta ggttttgcgc aaagctactc gccctcctca gcgtatggaa
     6901 ccagactctt ctttatcttt ctgacgccgc cccgcggatc cggcacatcc cctgcgtatc
     6961 tgggaatgag atgaacgtgg agatggaaga ttgtctgtcc agcagcagca cccacattta
     7021 cccctatatt gtagccatcc ggatgaaaac gttcatccag tttttctttg gcctttgcaa
     7081 gcagaaggct tattgcgttc ttctcatctt gagtggcatc gaaataggtc tcaacgtgcc
     7141 gtttgggaat gatgagaaca tgcccctcat tgaccggata ggcatcgtag aatgcaagag
     7201 ccagatcgtt ttcggcaatc accttttcct tcggcaaatt gcagaatatg cactgaggca
     7261 agaaccccac ctctttctag ttcagtcctt aaaccggcgt cggaagtagt cgattcgtcg
     7321 gtatgagaga atatcctgca cgtgttctgc aaggtgcggc gaaagatacg gctgcacctg
     7381 agaatcgata tacatcacac gatttatttc atcatagtgg aaaaactggc ctcgcgacaa
     7441 gaatctgacc gggtttttcc tggccagttc ggcgaaatcg tcagctgtcc attgacgcca
     7501 cttgcgattg ctcttatccc tgaaatcctt ctgatgcacg ggatggtcga cgtagaaacg
     7561 catcattctt tcaccgatct cgtccagtct tgcctgcgcc acaatatcgc cgtcacgcaa
     7621 aaatgccgct attgtcggca gtttgtatgc cttagtcatc cgggtctgtt caatctcgac
     7681 gaggaatgcc tctgccggcg tatcgagcca actggcctcc tcaggttcaa gctcgccaac
     7741 ggattcgagg aacctgagcc atccatccct gagatactct ctcatcggta tgtcgctgcc
     7801 ctcatagatg tccactcggt tcggcctttt acccagggag tgtttgattt tccaataagt
     7861 atcctgcatt ctcactggca aggggtcgcg cgccgccaat tcatcgaaca agtcgatgac
     7921 tcgtaagtca aagtttacag tgcacccttc cgggaattcg taatcttcta ttcgcagaca
     7981 ccttgaaaat tcatgggtcc acgggttatc cccggcaagg agaaggggaa tatggtgagc
     8041 cctcttgtag ttgccgataa agtcgatcac cgtaagatga tgtttgcctg gatattttcg
     8101 caatcccctg cctagctgct gaaggaatat cacatatgac tcggtgggcc gcagaaacat
     8161 cacagtatcg agggaaggaa catcaacacc ttcattgaac atgtcgacgg caaatatcac
     8221 tttgattcgg ccttcctcta gtgcctctat cgcgttatct ctttcaagcg cgtatccatt
     8281 gcctggctct ccactatgaa ctgcagccga aggaatcccg tgagcagaga agtattccgc
     8341 catgtattcc gcatgcccga tggaaacgca aaagcccaaa cactgctggc ccgcgagttt
     8401 gcgatagtgc tccaaaacca agtctgctcg ctctacatgc gatagctgct tctcaagatc
     8461 ttcaataaca taagaaccat tggctattct cacatcatca tagtttgtag ggtcatagat
     8521 cgcatagtaa tggaacggca ccaacaagtc cctttcgatg gattgcttga gtgagatctc
     8581 atagacgaca ttgtcatcac ataaagagaa gatgtcctta ttgtccatcc taaatggcgt
     8641 ggcagtcagg ccgagcagga aacgcggctc gaaatgctcc attaccttgc ggtaggtatc
     8701 ggctgcggca tggtgaaact cgtccactac gatgtagtcg aagtagtcct tggggaatac
     8761 atccaaatgc tcatcacggc tgagggtctg cacagtggaa aaatagatgc atgcgccctc
     8821 atctttttgc tcgcctgcgt aaagcccgaa gtgggattcg ggccggacgg acctgaatac
     8881 ttcgtaggcc tgatcgagta tttgctgccg atgcgcgagg aaaagcactc gctcgtattc
     8941 gagcgaatca aaagctgcca ggtgagtctt gccgacgccg gtggctgcaa caaccactcc
     9001 tttggatgcg ccttcatcgc gtgcctgctt caggtagtat agagcttcta tctgagcatc
     9061 ccggggctca ggcttcccag tccagaccgt ctgggtactc gtctcgacat cccgaatcag
     9121 cactggtttc ttccaagcta aagcgtattc ctttagcact tccgcagtgg ccggctgcgc
     9181 gtatttgtgg aataactcgt caaaggtgtc gctgaatttg tggtattcgc tcgggtgttc
     9241 gctacggaag aaacggtaat tccactcgat cccagtggtc agggcggagc gtgagaggtt
     9301 ggatgatccc acgaaaacct ctgcctcgtc cccgtggtca aaaatgtagg tcttgggatg
     9361 gaaagcttca atgctcccgt tgtaaaatct catgtctatg gagccgccta atctgtcata
     9421 aagaaggtac agggctgaag gttgcgttat ggacagatac tgccctgtca gaagctctat
     9481 agacacgcct cggctagccg cctgctgaag acttggcagg atgagcttca ccccagattc
     9541 catgacaaat gccacaatca gccgtatcct gtcggctctt ccgattgccg cctccaaacg
     9601 tggcaatagg tgatcatcca gaccagttat ggcattgatc acaaaaccca cctcacaaca
     9661 gactcaagca cactccatga gtcgaatagc tgcatccacc tgtctataga ttatcgcgca
     9721 agaaagccag catggactcg agtgactccc taacccgcgc gtcccccatc tggcggtcga
     9781 aggagtgccc taggccggga agggttatca gctcgttttt tactcccgca tgagtcagtg
     9841 cattggccat ttttacggac tccgtgcaat ccacatcttt gtcttcctcg ccatgcatca
     9901 acagtgtagg ggggtaatcc gaccttacat tgtgcatggg cgagtattcc ttcaggactc
     9961 tgtcatctcg gttgaggcca accacttcgc gcatccagat accctgttgg cggcagtaga
    10021 tatagaacgg caaacgttct tcgccgcttc cggatataac attgcttcct atgcattcat
    10081 atgcccgttt cttcgaaact tcgggcattg acagatgata cccacttggc ttattccccc
    10141 aatcagtgag aagatcgcca aatccaaagt acgaaacgac agctttcggc tttcgggcaa
    10201 gggtcccgct gagcaacgcg agatacgcgc ctgccgacct gccgaataca gcgactcggt
    10261 cagggtcgat gccaaagagc ctggggcagt catccaatac ccagctcaga gcgtcgcaca
    10321 catcttcaac aatggacggc agctttgttt ggggggcaag acggtggtct atggatatca
    10381 cagtataccc ctgacccaca aaggcagaga tttgctccgg cacaacccgc ttccgcgatc
    10441 cccatatcaa acccccgcca tgtatgtaca ctacagcggg agagcttgga atggatgaac
    10501 catagacatc agcacatatg ctgcatccgt caacgcgttt gtattccaca gtctggacat
    10561 gcattccaaa cgaaacctcc ctcatcttga gccatcagtc tacattctcg cctacccgtc
    10621 ccactccgca accatgaact tcacatacac cgctcccggc cgaagcaagg cttatgtaaa
    10681 atctgtaaca accacacaag gctgcacaat actgagcatc ctagtggttg cgctacaacc
    10741 tcaatccgcc cacttgcgca aagcgtaaaa tggggccgct agcctgcggg agaatctgaa
    10801 cagtctttgc tacatagccc actgcagctc cacagtccct aggcatacat actccacggt
    10861 gttctcaatt cctttactaa cgcccgaata tcgagactac cttcctgaca cgttcagcat
    10921 tgaaccagtg ccagatgtcg aaatacactg ggatgtggta gcatgttctc ggaggtgcca
    10981 gttgatgcct tatgtggggc tcgcagtagg aactggccta ttgctcatcg ccgtcaggca
    11041 caaagtaaat gtgggacttg cagccacaat agccgcgctg gtcgttacag ctatgagcgg
    11101 caacggaatc gccatgcttg ctggagcatt gcacgataca tttattgatc cgaacggcct
    11161 caccttgctt gtttccatcg ccctcatcac catgttaggg agtgtaatga gaacctcggg
    11221 agcaaccgac gccttggcac agggaataag aagcctagtc aaagatgatc gctgggttgt
    11281 cttcctcatc ccttcactta tgggcttcct cagcgttcca ggggctgcag tcttctccgc
    11341 gccaatcatc gattctgttg gagatgcctt gggcatgagc cgagaacaga aagtgactgc
    11401 caacatcatg ttcagacatg catggtatta catctatccc ctctccacaa cccttctcct
    11461 cacccaacaa gtctcgggcg taccatatag cgccttcgca ttgccaggct ccattgcctg
    11521 cataatagta attctatcca cagccaagct ggtgttcccg caggaagcac cgccaatgga
    11581 gccttcgggt acacactcaa gaaccgcctt tggcttagac gctcaatcca ggctgccgag
    11641 cgcagtaata tcccgggccc gtacaacatt cccgatgatc ctgatatcta tagctccgat
    11701 catcgtagtt ctgcttttcg ccctggcaac cccagtcagt ttcccgctgg ctgtagcatt
    11761 tagcgtggca acaggggtct ttgtggtgca accgggcaaa ccctactggc aagaggtaaa
    11821 cagaagattt agggaaggcg tagtgcgcgg catcgattgg cccgtagttg cgacagtggc
    11881 aggcaccgtt cttttgggca aaacagtggc caactcgcca gccctagata tagcaagtgc
    11941 cagcatgcag acaagcccgg catccctgct cttgttcggg atcgccatgc ccattgcagc
    12001 tgccttcgtg ctaggctcac ataccgccgc cgtagccatc acagcccccc ttctcctgcc
    12061 cctaatcgca gacctgccga ctcccttcgg cttcgtcgtc gccctcttca tgtcatcgtt
    12121 ttcagggtat ctcgtctccc ccgcacatgt ctgtttaacc gctacaacac aatatctcgg
    12181 ggctgacttg ggggaaagct ggcgactatt aataataccg agcggtctca gccttctatt
    12241 cacccttgcg tattggggat ggaatgcgct tttcaacctc atgtaggctc ctctgttcct
    12301 taccactcca ctgccccaca ggttgcgcac cccttcgctc tagaacactc tcacatgctc
    12361 aaccgggcca taggtttctc tcgcaaaacc atatctacca agtcactccc aggctgaatt
    12421 gatccggata agtccacctc aatgtgatac cattaccaac gtcatacctg acagatgtct
    12481 gctgctcaac cttatggtcg aatctgagtt tgcgcaattc aaaaaccatc ctgccagaaa
    12541 aaagttccga cctggtcgaa aaggtgccgc atggtgataa tgaagatgcc ctatcagcaa
    12601 ctatccccgc cccaatccag atcctcgtgt tgaaagtacg gaacacatcg atctcacaca
    12661 gaaaatcgct agatcccttc ccgagctcta actgcagcac caagctcgga gcaggcgata
    12721 gggtacaccc aaccccggct gacgaaagag acaagctgtg tggttccctt tcggcacgca
    12781 gagtgttgac tgtgtcaatg cccaacacat cgctgtacag agtctgcagc cagatgctat
    12841 caggcacagg atcgaggttg atgtcagggc ccacaagtag caccggcact gtgcagtcag
    12901 acgctgcaac acctttgtga cgggcaacaa atccgcttgc gttaggcatc cctttcggag
    12961 gattagtcac tacaaccacg tctccactgt agtcaccctg catgtatctg acaatctgga
    13021 ctggcacagc cggccattgg caccgcgcgg tcagatccaa ccattccgag tcggttagag
    13081 cttcacccga atacccgagg tcaacatagc cgaacagatc gcttccctca caggagtagg
    13141 caacccgctc gtcatcataa tcgatcatca ctgtcccttt gtcatggcag ccgactatcc
    13201 ggccctctcc gtcacgccaa aaggcaaaat caacctcgtg ccgctctcca agagcccttg
    13261 caacttcggg ggcatgccgc tcgtctttga gatacacgtt ggggtagaag cagagcagcg
    13321 ccttgtcacc tgcaacctcg gccatggcac gctccgtgag gacatactca tcaatcatac
    13381 tcatcccgtg gtcacagtag aggacaagat ttagactgcc cggcgagtct ttcgtataac
    13441 gcaccaacgg agccagcatg ctgtcgagac gcattgcgct ggatttctgc gcatcgatgc
    13501 cctttatgtg gcccacggtg tcagatgcca accacaggaa ttcaactaca ccataatccc
    13561 gtaaaagccg ttcgatgttc gccagcgaca gacgggacaa cgcgtcaagc cctagcacgc
    13621 ctgtgagaag ctgaccaaac gcataccgcg gcgcccacga ccaaagccct ataacattgt
    13681 caaggtaggt cttgtattca ccggtatggc ggttgattga agtccacgaa atgggatcat
    13741 ccgaactcgc cggagccccc tgctttaacc gggggtagat catctcggta ccaggcataa
    13801 atggggaaat agcatacggg atgaccttgc cttcggaaaa cagctccgaa atgttgggaa
    13861 ggaggccggc gtcatacatc tcaaagaaca cctgtgacga gatggcgtca aggtggatga
    13921 taaagtacgg cttgtgctct tcaactccag tcaaatcttg cactgcctcc ggcgccgcta
    13981 acgctcctga agcactcact gccaacaaaa gcactgctag tgccagaata catgttgctc
    14041 tcaacatagt taatctcaag aaaatacctc cgaaatagtg caatatggcc tgtccctatt
    14101 cggtttgagt ctgtattaca tccgaagaga atcgcatgat ggctgttccg tcaatacccc
    14161 gaaatccttc tgtcaaattc acaccactac ttcagacttt aacaggctct ttcgattgcc
    14221 gtctcatgct ctctcatccg cttatcattg ttcatactcc attaagtctc agtctccttt
    14281 cagctgccga gacctcgttt ggggatattc acccactgcc ctgaattccc acacccaaat
    14341 ctacgcctcc tcgatggcaa cgcgtttcaa tcaagtaagg tggggccgac ctcatgtttt
    14401 gcgaggtcaa tcagtcctat ctcatcgaac taaccctgag aaagcaccat gttggccaga
    14461 gggcttgccg agttccgcca tatcctcgac actcattcca tagctgcgag gactcagcct
    14521 gatcaagtcc gttcaatcca cgcacctgga tggagtgcga cgacatggcc aaaaccttcc
    14581 tagtctatct gggctttaac gatgaattcc atgaatccct ccgaaaagcc ataccccctt
    14641 gacatttgtc caagcgatgg ctgcagatat gtcggctacc cggcttgcgc agacctccgg
    14701 gaaaaatcgc caccacttag ggttcgcagc aggatgttgg gtataaccag gctccaacac
    14761 gccccgattt ccttgatgac tcagaagaac tatcctctac gcccaacacc tcagctcctg
    14821 tgcctagggc cccaacctca tccctcatcc cctgccggcc caacgctgcc tcgcaacaac
    14881 cgactcaagg ttaaagtgcc ccccaccaac agggtaaagt agtaggtgag cagccgccaa
    14941 agggctacag ccacaccgat cagatccggc ggaacaacca tcctgaacag ctcggcaaac
    15001 cccagctcgg ccgcgccgct cgccccggga gtaggaacat acgatgaaac catatgaaag
    15061 agaattacat tggccatcac ttgctcgcta ggcgccctaa ccccgagccc agcgaggatc
    15121 atcgtaggca ccagaataag ggcaagccat ccggccacac tcagatagat cactcccagc
    15181 agggtccaac gttcacactc ccaatagtgc gcaagtgcga cctgaaactc ggagatccac
    15241 ttccttatcg atttgagaac ccgttcactt ccatcaggtt ttaacagcct ggcaacaaat
    15301 cggctgcgaa gaatcggagc aataacagtc tcgatcagct gtgggtcatt catcaggaac
    15361 agcgagaaaa ggaagaatcc cacgtacagc gaggacgcag tcaacaacac cgctttcatc
    15421 tgctgcggca cacgccaacc tatgccggac aggacgatcc atgaagacgc agtcaatgcc
    15481 acaataacct ttgcaagagc gttgcagaag gtttttaccg ctacgactgc cgatgcctgt
    15541 cccacgctga ggccctcatt cctcaacaag tagatctgca tgggttctcc gcctgcattg
    15601 aacggcatca agctggccac aaaaacgccg gttatggcga cacgaatccc gctcaaaaaa
    15661 ccgagtttct cgcctatggc tcggattagg gccgacattc ggaggctatc gaaaacccaa
    15721 gtcgccgtca cgagcgaaag cgccacgata accacaaccg ggttgattct catcaaatcc
    15781 gaccaagtga cctttgctcc tgatatctta aacaccacga ggagagccac tgtcccgaag
    15841 atgagagcga taatgaggcc gcgcaccaat cgcgcatcca ttttgccgcc aattcctccc
    15901 ccgcactaca ccgtgggttg atagcaccac cctaggtgca accctactcc gcaaaccgtc
    15961 cttgatatca tcgataatct gccccatcga cgcctctcat aaagatagga gccggtcagc
    16021 agattgacag accatgcaag tatacgactg gatttggact catattcaaa aaaccatcct
    16081 cgaccgcgaa atgcgcgaca accaccgaca acacgcgccc gcatcttccc ggaaccctgg
    16141 gtggaaacag tggtcccgca gtatgcgcct cgatcagtaa gggccctgat cagaagtgaa
    16201 gctgatccag catggagacg gcaaaggttg ggccgaaaac cagccagggt cccggaaact
    16261 gtccttgatg tgatgcctga agcaaaacgt ccttccgcaa cccaattcca atcccccgca
    16321 gcacccggcg agccaaaagg tcagatcgag atgaacgggt atctatatat aagcaagcat
    16381 cggtgaattc cgcttcaggg acgccgattt ccggcaaggt tttggcgaat catcacatag
    16441 accgcttcga ccggcaactg acgcttttag aacacaagcc agtcctcttt gtgacatgtt
    16501 aaccactacc ttgcttgccc atagctagag gcagctaata cacgacgaag tggagggaca
    16561 gaattgtctg ataatgatga gaagatcgta agccctattc aagtcggaat tgctttatcc
    16621 catggcgtgc tcccggcaaa caggcccgga aaactctggg ctctggtcga gctttcggcg
    16681 tcaaaccaag ccaccgaaga ggccaaagca gtcagcgaca agtccgactc gcgnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacacct tgcgaaagct caccgaggag
    16861 atcagcaaaa ccaccccacg acatacacta agcgaaacgc gcaagaacat gcggtcccaa
    16921 agctaccaaa taaggcacag ccaatctgat tacggatcga agaaggatcg ctgaaccaga
    16981 ccggcagcgc gattgctgca gagaaactcg ccacggggat cataggcagt atcatacaca
    17041 aacagcccta caagccgcct tatggcggcg tgcagggcta gttcttcagt cttgcaagcc
    17101 tggggcaggc cccctacccg gccacagtct caggctccgg atactccacc ccgaacagct
    17161 ctacagtcac cttcttcatt ctcacaggcg acagcggctt atcgctccgg tcgcacggga
    17221 ctttcgcaat gcgttccact tcctcgtagc ccgacacgac tttcccaaaa gccgcatacc
    17281 cgccatcaag gtgcggcgat gttccaacca tgatgaaaaa ctgcgaaccc gcagagtcgg
    17341 gatgcgcagc gcgcgccatg gaaaggactc cgggtacatg cttgaggtca ttcgcaaaac
    17401 cgttgtcctc aaattccccc cgaatactgt acccggggcc tccggttccg acccccttgg
    17461 ggcatccgcc ctggataacg aagcccggga tcacccgatg gaaggtgagt ccatcgtaga
    17521 agcctttgct cacaaggctg atgaaattgt ttaccgtgtt aggcgcaaca tccggataca
    17581 gctctgcctc tattacagct ccattttcaa gttctatgga aacaagtggt aacaggttct
    17641 cttgcatgtg ccttcctcct cgcacaacat aatggtaaac ctgccatggc tagatcgatc
    17701 acaagcacca tcataacagc tgaagcgcgg gaaatgaagc tcgacggccc aaagctgcat
    17761 ttcctggatc tggtccaatc agaatcttct gatacgccat accacgttca gaggtgtatc
    17821 cggcccaacg ccgccacctt catcaacttg agcaggaata atcgaagctt tgccatcata
    17881 acactgctag cccgacctac gatggtcacg acaacggggg cgctcagagt tggccactta
    17941 gactgcactt gccatgcaaa tagccgtgta acagcaatcc ccaaatgtga tttgattctg
    18001 ctattgctga agcagcatca agcaagggga gtcgcttcaa atggaccacc tcgagccaca
    18061 caaatgattg ccacggcgac ctcgtgggaa tatagcagat gagccgttaa cctgtcccct
    18121 atggtagaag tggcccgatc caagacactt ggttgtctgc tatactacat acgtgaagga
    18181 atcatggttc atatgcgcag cgatacactc cgtctagtat cgctgatccg aatggggggc
    18241 gttgatatgg aggagggcga tgtcaaatcc cttgcctcag ctatgggcaa aagagccgtc
    18301 aatatcggga tgaaaaacga cagcatcaaa ctccttcgtt cagccatgaa gggccgccac
    18361 aaatccctca ttgcagtgga aggcatatgg ctgaatgcaa aggcgctcac cgcccgggca
    18421 cgcatcgaaa cattgtacgt ctgccctaca atgattcata cgccggaagg ggcggacctc
    18481 ctttccagac tggtcgacca gacgatgggt gagatctgca taatctccga aaacgtctac
    18541 cacaggctga gcgagccgaa atcggacgaa ggcgtaatct cactctgcag attgccagaa
    18601 atcacgctca acagtgttcg tccgcgcact agcgacttag tgctcgtttt ggacggtctg
    18661 gagagcccgg gcaatgtggg gacgataata cgcacagcag aggccgccgg cgcttgtgcc
    18721 gcagtcatct gccatgggca agcaaatatt accaatcccc gaacagtgcg gtcgagcctg
    18781 tgcagtgttc tgagcctccc tgtattggag gcggatatgg aagacatcat acggtggctc
    18841 cgtatgcacg aattcgcgct ctacttcgca gacgcctctg gcggcgtcgt ctacagcgac
    18901 atagagtatg ccccgagatc tgcattggtg gtcgggaacg agaaatacgg gataaacccg
    18961 gactggtatg aaaccgaaca cctgtcggtg tacatcccga tgatgggcac agtcgattcc
    19021 ctaaacgctg gcgtcgccgc ctctatactc gcctacgaaa ttcgcagcca attcgggcac
    19081 actcgcgcac ggtctcatat gaaagcttaa cggtgtgaag gatcctatta tcccgtgcat
    19141 gcatctttgt cttctctcct ccaggccata cgctcgggct ctggcaatca aggcaatggg
    19201 cttgtagctg tttcaggcac aaacaatccc aatccaaaat ggcatgcata gcccaaagcc
    19261 acaggcccca tcaaagggcc atcgaacatg agcttgaatt tgaaagctct cttcaccgga
    19321 tctggggcat tgccataggc tcggactatt cgatataccc gccttgaagc agtcgcgcca
    19381 agcagctgca tatcaatgag cagcggcaga ctgcaattgt cgtgtacacg ctgatcccat
    19441 tcccgggcaa tctgctcttc agggccgtcc tgttgtctgc cgttcttggt tagcttggtc
    19501 tctccggccc gtgtcctctt cgggtggcgg gtcatgacgt aaggaacagc agaaatccaa
    19561 acacgagaag gcccaaagat cctgcccagc tgcgcgatgt tctccatgtt cgccacgccc
    19621 gacagcctga gctccatggg ctcgcaataa tgtccaagat agacttccct cactctggca
    19681 atcgcgtcga tttcgagaga gttgaaccct cgaacgctat acacgaatac tgtgtcaatc
    19741 aacccatctc cgtcgccgtc atcagccaaa taaaaagcgt gctgatgacc ggagagcacc
    19801 tttcccgact ggtcctttcc cgacaggacg ggcgatactg ctccgccatg ggcctttcca
    19861 tactgactca taatcgcccg tctgcacatc tcgcccacaa gcaacgtctc ttctacaggc
    19921 ggacgaaagc aaggctccac actgcatcgg actccaacaa tgccaatcac ttatttgcac
    19981 tcccaggaaa ccgtcttgtc ctcgcttcct gcctcacagc ttccaagttg attatctctg
    20041 ctacttcgaa ttgtgtgggc acgctgcccg ccacaggcct aatgcgcacg caggcgggaa
    20101 agtgccccat acgtccatga agttcggcca ctaccaacgc cgctgcgttg tgcaggccgg
    20161 gcaagttcac taacactgaa ttggtttgcc agtcctcagc cagaacagga acggcatcga
    20221 taatacctgc aatctgcgga gcaaacgcct tgtcctgatc gagctgacat ggtatatcgt
    20281 acactgcatc tattgtctgg ccagtcagag cctctatccg acgctgctgt tcttcactga
    20341 gcgggtgcga gaagttcact actatcattg ctcatcctcc aaaggcgcta gtatccccag
    20401 ggctctatct tccagtcagc cggccgattc atataaggcc tcaagtcagc ccaaactcgc
    20461 cgaactcggt tagccatttc atctacatcc aaattatctg tagcctcgtg gccatgatga
    20521 tgaagattgt ttcgcagctc ccatacgcgg tcctttattc tgtacagttc ccgcgacacc
    20581 catctgcctt tgttcgccaa atcttcggcg ctctttcttt cctcccgagt ccgccacttg
    20641 tccctgtcgc cgttgtagta tatgaccatc aatatgaccc attcccgcat catgccgacg
    20701 gaattgacgg catggcctgc ctcgatatat ctatctataa cccgaacctg tctacgaatc
    20761 tcttcccaac tgagtgtatc ggggactcgg tctccgcttg cgggagcaaa gtccgccgcc
    20821 agccttgaaa cctccccaaa caactcatca ggcagcaaga cagttgactc aagtgtttcg
    20881 ggcaatggag ggctcacctg cttacggagg tcgacagctc gctcagcaag ctcgataggc
    20941 aagcccagtt cgaaggcgtt tgcaaatctc gttatcgact ctatgatccc gtctgcatcg
    21001 tcagagcccc cggccgcccg cttgaccatg tccgccagct tctgcggcat ataggtgtcg
    21061 cgaaagacgc gcaccgcgta cacccagtcc ataagctcga ggaaaatgct catatccacc
    21121 aaaggcgtgc ggggagcgga atttggctcc gccttgccca acatgccata gtaaagccct
    21181 tctattgtca ccgacggccg tagaaagctc agatactgag cggcaactga aaagacgaac
    21241 gggctcgacc gaaagccatg agttatatcg agcgtcaaat cgcactcatc cggaatagcc
    21301 tgcagcagta ttctgactat atccttcagc tcttcctcgt ccttgccatt gggaacctca
    21361 agcggactca tatcgatgcc aagcttggca aaatcggatt ctattcccgc cactctctcg
    21421 tccatgacct cctgggtgca gagcgcgatc acattatcgg gatattcctt accgcgtacc
    21481 tttttttcca acttcaccag ggcgattggc gctaagttcg tcgtctcaag cacgtcgggg
    21541 ctctcaggca ggaagtaatc tgcctccctc ggcatggtcc ccggcgcaaa aaccaatact
    21601 ctttcccgag ccattgatcc tcaaacctcc cccaacactc tctttacctc cgctactttt
    21661 tctctctgct tcttgctgag cttgccagac caacgcccaa cagccatcca aacatctctg
    21721 agcgtctgcg caacgaccag cttgtcgtca tcactcatcg tactcatctc gtaatacaag
    21781 tccgtagttg tcttgatgat cgtatcggtc tgtccttctt tctcaacggc gatctccagt
    21841 tcacgaagct tcctctccag aggagacagc ttctcaagct cggcgttctc ttcttgcctc
    21901 cgaaattcgg caaacacctc atccgtcttg tcctgaaagg actcgaacac tccgtagcca
    21961 acagctgtct tcgcaccgca gccgataacc tcgagggcct tctgaagcaa ttcgattcct
    22021 tctgccaaat cgtcttcagt gcacctcgga tccgcaggag ccaacgtgaa ctcgaaccat
    22081 tgcccagccg gaaccacaag gaagttgact ggcaccggcg agtaataatc gcccgggagg
    22141 tttttttgag ggtcggagta gtaggggcca tagtgcggat tcataatgtc catttccagc
    22201 ctcggcggag tctccggcac ggcatcgaag aacatcagcc gtcccatgcc cactgtctca
    22261 gtgtcaccaa aaagctccgc agctcgtgcc ctcgcgcgct cagcagccac atcgtctgcc
    22321 ccgccaagcc agtgggtaag ccaagcccta accatgccct ttacggagga actgggcaag
    22381 tatggaactc ccagtgtgcg gtgccagata aatccggctt ccaatgggtg cgaggcgcct
    22441 agccctgaca cgaatcgcca cacagtcctg gcgcgaacaa ccttgccgcc cagctcccac
    22501 aggagcctgt atcttcgctc ccgatagtcc cggagaaggc tggaacagta gttgcccggg
    22561 gatgtagcaa accgggcgta gaaacgctgc tttccttttg ggtccatgga gacccttact
    22621 ccttctgcat cgccggtttt cgaccattcg tctatgtact tgtcatactc cagcccaaga
    22681 ttggaatcct gacggtgctg tagccactct tctgcgtctt tgtagagagg cagcatctat
    22741 gatcgcccct cccctccgtg ggggcgcccg ccgcccacat cagagccctt ttcctccaga
    22801 ccttcgccgg ctccaatata ggcatcggcg aacttcttga gccacgccaa catagcccac
    22861 gtttcctcct gggcctgccg gtaaaggtat ctgtccccct gaatcagctg gttcatcaag
    22921 tcagccctgg gccccgcata tagtttacgc ttgtccacaa cccattctgc gatcatgtcg
    22981 tacacgatat actccggccc cgatctcctc ccttcgtcct tctgaagcaa aaacgccaag
    23041 gtctggccta acccgttagt caacaccatt gtcggcatct tcctgatgtt catggcgaca
    23101 tccttggcat cgtttggatg gtctttgcag actgagttga cccgctccca agcgaagaga
    23161 gcacgctcct gttctagcgt gcgcattatc gatcccccct cacaaggaag gctgcgcacc
    23221 aaccatgccc caatgtctcg ttcccgccaa gctgtatgaa gtgatttgcc agcacctcgc
    23281 ggtcgagctt gtccattacc gcgttcccgg ttgaaagctg atcttgggct ccgcctccct
    23341 tggccgcact ggccatgagc gggatgtaga aaacagtctc gggaggcaat gtctcttcat
    23401 accagagatt accctgttcg atcttgccat cgggcccgat cgtcttgtct gtagtcttat
    23461 tggaagtcaa ttgggtcctg gcggaaactt gcgtggcata gcgaacgaaa tatgcgaatt
    23521 cgtcgtcgct caaaaccgca atatcgcttt tgagcttctc attgaaagca gcgcctatta
    23581 tggaggcatc gaacttttct gctaagaact gagctatggc tgatacggct ggatcttttt
    23641 gaggctcgaa atccatctct tccaggtaga gacgggacga agtcttgcat tcctgcggca
    23701 ctagcgcccg ggcgccgtcg acattgcatg gtccatccca accggggttg atgccgactg
    23761 tcttgaggtc ccgcagaaaa cgcgcaatgg ctagcggact cgtcacccaa aagaacgacc
    23821 tggtaagcga tctgaccgga aaaaggatca acttggcgtc gcctacagtg agggcaccgg
    23881 cgaaatgcct ttccttactg cggggctccc ctgctgcagg acggtagact tccggtccaa
    23941 ataggccgtc caccacgtcc tgagaaaccg ttccttcggc ggtctcgcgg agacttccct
    24001 taacactgga cgcaggaatc attgggtagt ccgtgtgcac ctcgcgctga acgggcaggt
    24061 cgatcactcc ggtgcttgct cccgctcccg ggcgaagcca gctttggcca tagagaaaca
    24121 gaactccttt tgcggtatcc aaaactacca cctcccgact actgcatggc cgaaacctat
    24181 gcgagtgtcc attccaattt tggcgtcatg gaacgccgcc acaacctccg ctcctgtgtg
    24241 aggagttgtg aagtagtaga cagctccggc gggcacgtaa gccaatcgcg gcttgggctg
    24301 gttgagcacc atattccaac cgcctatgcg aacaggccta tccgagcaca cgctcacaag
    24361 tgtgcaccgc atgtctcgca aaatcccttc ccagaccagt gcgccgtcac gttcgcactg
    24421 gtcaaatcct ggcggcagcc atccgtcacc atcatctcca gcgcagaact ggacaggggt
    24481 cgtgaagacc gctttgaaac cccgctcgcc gaacaaactc ggaccccgat cgatcgcttc
    24541 agcgacgccg tctgcaggga acggcttcga acgctttact ctcaggctcg taaaacgccc
    24601 ttcccctcca agcctgacca cgttgggaac tggcgtatcc ggacatccgc ggacacgcac
    24661 gcccattccg aacttctcag ttggcccatg caaaaagcgg atatgttcaa tcgcatagag
    24721 cattccttct tgcgtagcgc cggtagctct ctgtctcgct attccgatcc tctcctcccg
    24781 caacaggaac gcccgtcctt gctctgaagc tccatccttc ttagccgaat agtaggcctc
    24841 atctgggctg acgtcagccc ccaaaagata gcccaaaagc ccgttctcgc ttatccatac
    24901 atcagacaaa gacttacaac gcacatcaga tgagggcata aagactctac caatgtcggt
    24961 catgacgggc tctggggacg gatgcaaacg gccgaactcg atctcggaag caacagattc
    25021 tacctgcacc aaatcagatg gggctctgta tagacgctcc actccggttt ctgtctcccg
    25081 cgtcacgaac ggaccaatca ggtccattcc cgcgtcttca ggctcatctg ggctgccact
    25141 catggctttc aagacctcgc agtcttcctg ggcacaaccg cacacgctgc accgaccttg
    25201 cgcatattcc ccgaaatcca ggccgtggcc tcgcattatg gccgtgcgca ctgcgccgtg
    25261 cagtacattg cagctgggcg ggaaactcga cggaaggaac gtgtcttctc cggcagtgaa
    25321 cggcgttccg tcccgaaaga agtagctttc ctcgcagcca aagagaaaga gcttttccgc
    25381 catgtctact tcaccccttt cgcctctgcc agaaacctca caatcggcaa tgcttcaggc
    25441 tgcaaaatgt ggtcgcgaat cttgccggac tcccgcggct cccatctaca caagtcgtat
    25501 aattccgtca tcctccggac agattcatcc caatcgatct caagcaaggc acctggccca
    25561 cgcaggtatt cggctgccaa gacagggatc atgtccgccg cctcaaaggc aggcccctca
    25621 acaccgtgtt ccttgactga acgggcaaag ggttccagca gttctgcggc gctgtacagg
    25681 aacctggagg agtagccctc atcttcggag ccgaatatct ggccactcag ttcgtcaatg
    25741 cgttcggccc acgtctgccc gtccttccgg ttcacagcac cgcagcgtga gttgtcttcc
    25801 catggctttg cgacgacaag cggtgaaccc cctctcttcc gcacctcgat cacaaaggca
    25861 tcgcggccta ttccttcttt ggccacgtca tccaagagct gatgtgcagt cgcaagtgcg
    25921 gcctgcagcg ggcttttcac gtgcacgcac aggatggcag cggagatggt catgggaacc
    25981 tcggccaact tgctgctttc aacaaacatc cggcggaggc ggacagcgca attgagggcc
    26041 tcatcgacag ggaggagcgc caggacatcg tcgccgccag cgtacacaac tttgccatgg
    26101 tattcgcgct ccacaatctg cggcaccgca cggctaaaac ctgagatggc cctggatatc
    26161 tggcctgtcc gttcagggtt gctgctaagg tgcttgccca tgctgtctcc atccataaaa
    26221 aggatcgcgt agtatggaaa ggccagatct ccagagtcag agcttgcctt ctcacctggc
    26281 ctgcacgcca ggtcatgcgt ggacggaaag ctgacagtta caggccagcc aattgcctct
    26341 tgcgcgatgt aggggaataa ccttttcacc atgcagacag cgcagaggcg ctctttttgg
    26401 tcaggatcga gcgcgagagc ccatccgcca ctgccactct cgccatttat cgattcgatg
    26461 accccacccg ctccatgcca tatctgacgc gcctgccatc tgggcaattc cgctccaaat
    26521 gcaatcactc gctcgccaca catggtgcag acttctccgg tctcatcgaa catgtgcatc
    26581 tgtcgccagt tctttcgttg atcaaccccg gacggcccag ccccggcaac ccagtagagt
    26641 tcccaatggt tttcaatctg acgagaccat gtcggcggga gagaatcttg gcaagcgagt
    26701 tcgggcagaa gttcataata tctgaaggac cgcctcagac gattgcagac tgtcttccat
    26761 acctcaagag ccaaactctg ccaagcgctc tggacagcct gcgcagccgc cttccctgca
    26821 gataccgggt catgacactc ggcgctgaac cggttgggaa gggatgcaat ccgagcgcct
    26881 tcatcggacc cactctgcgg cgtcgccctc cccatcaccg cagcgagaag cggatcgtca
    26941 tcaatgtggg ggaatatgat tctgtttgcg ttttgctccg gtgtttggcc gggaaggcaa
    27001 ggtccagctt ggagaacggc cttgatggca tgggcagtca gataggagat gaggtatgag
    27061 ccagcccaaa aatccctggt gcgcctggcc tgcccgacaa atccctgcac gggggcgatg
    27121 gtgaaatcga gaatccgctc cggatgtggc tgatgcccca gcccacacga cgcagatact
    27181 gaattcttgt ctctgccaat cggttccacg gctatatcag cacctccttt gctccgggct
    27241 gcccagctat gtgatcaagg aagccttcaa cagctcccca gctagttgga ggagagactg
    27301 taatcgtccg ctgcttttgt gtccccatgc ttatcttctg tcctgcaggg agcaatggcg
    27361 caggcaggaa tgtcaacaca acggcgtact gcccgttctg caagagatgg acgtggatga
    27421 acaggggact tgcacgcctt gagaaatcct tattggcccc ctcaatgttg actttgtctt
    27481 tcgttgtgcc aaagaaatag ttgtggggca gcccgaacat ggccctataa ggggcttgtt
    27541 cggcagcgcc attctttaca tagcccgaca ccatacgcac atcccgaatt gcccagggat
    27601 aatcaggggg actagtcttg gggttgcgga gcatcatcat ctgctgtcca acagtactta
    27661 gagcctcctc ccacgatttg cctgccggca gcgctactac acgcgattcg ccactaaaag
    27721 atgtatatct agcttctcgg gattgaggaa tgttgagcgc agctacttca tcttgcaggc
    27781 gagacacaag gtcatcgaag tcggttggca agtcgtcggc aaacacaact gaaccccagc
    27841 cgcggcggct acgcgcccct agtccgccga agtagttcat cagcctaagg ctacgcaaca
    27901 ctccgtcttt cacatctgaa gaggcgctag gcttgaaaac gagtttaagc ttgatatcgg
    27961 tcccaggagg gatgaacggg cggctctgcc ccttgtttat ggcgccgtag cccaagtaag
    28021 ccaggggcgc tttcacgtga tcgagggtct gcccgctcag catagcggta tccatgcgga
    28081 tcagaaatgg tgcttggccg gaggccgcct tccccgaact gccgaagaga gcggcctcgg
    28141 tttccaggcc ttcgggccct gttgcccgat gccaaaatct catgactcct ttgatggaag
    28201 gtgggcgcaa ctccgccttg ctttgcgtgg cactgccgag aaagagtggc gtcacaactt
    28261 tgaggtcgat gtcctgagca aattccaaca caggctcagc catcccacgg cctccaatca
    28321 tcgacattat cgaaagcaat actgtagaca agtcatttcc ttaatgtttc gtcaaaacgc
    28381 tcagaagccc ttccaagaat gtgggaagtt tagaagaaat ctgcactccg gtgacaggca
    28441 ggcacagcct ctacccttca cgggttcgcg cttaagggtc gctgcttttg tgggagtaac
    28501 attctatcga gatcacggca atctgaggca tctcaacccc ggaaatctag acgtcctcag
    28561 agcaaaaaac tggtgcattc agtccaagca acgactgagt agcttcgaaa ggtagccgaa
    28621 cattctcgtg cagccctgat ccggatatct ggagatgtag actagtcggg acagtgtcat
    28681 gtggcccaat cgcagtattt gacgggaaaa acgaagcgtg attggcaaga aatactgggt
    28741 agctcccaca ccctttatag cagcccagat ggagggagtc tcacaggtcg cactaacagg
    28801 agggacgttc aggttgaaga tctgagcaac ggcgcccgac catctattca ttgctccagc
    28861 attgccaggt cagccagtgc atggcgttct cacaatgccc cgccttgcat ccagcggaag
    28921 actgctatgt tatgaaaatc gtggcctcag gcatgtgttc tagttatcca ctattacttt
    28981 gcgatccagg aaggagaaat ctcgcaacga gcgaatagta tgttttaata gggaggcgct
    29041 cacttgtcag atgcgggtaa tttcgagcac ctcagaacac gccagaatgc agggaggtgc
    29101 tcgaaacaca aacgcacagc tacacgtcgc gcatcccgcc ttgtagctga agaattgaat
    29161 agtttctgtc cggggcacag aaggcactcg aaatcgccaa ttagcgccca gctagatccc
    29221 cgcggaaaac gtggggggtc tccatggttg aaaaaccgtg gctccattga aacgttgact
    29281 atttcacagt gacatcgatg ctggtggcgc ccgtctccat ggttgaaaaa ccgtggctcc
    29341 attgaaacaa cccctggctt cacctcctcg tacctctcgg ccatcattgc ctgtctccat
    29401 ggttgaaaaa ccgtggctcc attgaaacat gacgtcattg gcgaagccat gaggggcggt
    29461 cgactgtctc catggttgaa aaaccgtggc tccattgaaa catcgatcca ctgggtcatt
    29521 cctttgacca ccaagttgga gtgtctccat ggttgaaaaa ccgtggctcc attgaaactc
    29581 aatggcatca gcctgacaga catacgcgcc ctttgacctg aacgtctcca tggttgaaaa
    29641 accgtggctc cattgaaaca ctacttagtc tggggctatg aactacaggc ttataggtct
    29701 ccatggttga aaaaccgtgg ctccattgaa acactcggat agtagacaca tgtgatctga
    29761 ccaccttgcg gtgtctccat ggttgaaaaa ccgtggctcc attgaaacct attagactag
    29821 ggctgcgatg gttgctatag cgatagcggg tctccatggt tgaaaaaccg tggctccatt
    29881 gaaacgccat tactgtgatt gacctaaggt tgcgtctggt gtgcgtctcc atggttgaaa
    29941 aaccgtggct ccattgaaac acttgtcaag tgccgccgcg agggcagcga tgcctagagt
    30001 ctccatggtt gaaaaaccgt ggctccattg aaacgggcgc atttcccatg ccgtcactac
    30061 tggcacggag aacgtctcca tggttgaaaa accgtggctc cattggaagg ccttctacct
    30121 gtgattattg caatgtcagg gttgttcgca tgcaaagccg gaaaatcagg cagcggtaag
    30181 aatatgacac atgaactggc tactgctcgg ctacataggc tgcttcagtt cataaggatt
    30241 cagagataca aagtcaatga gaatcctgat taccccaaga gcgtagaagc ccgtactgac
    30301 aggctgctcg gaagtattgt gttgtgcatc attggtgcgg caacataccc gcccgcaatt
    30361 gcgactatta tcatcggatc cgcagttagg aaccacgttg agaacaacat agctctgtaa
    30421 aagaaccatt acattaaaga ctatggctca gtttttccac agtgggcgct agtatatcga
    30481 ggaggcatgc cgttgcttac cagcgaaaaa tccgattata cgactgactc aagccctacc
    30541 gcggacatcg ttgtcagcga cgaaatgctg gaggatttca tattctgtcc aaggctagcg
    30601 catctcaagt gggcacaggg cgaagcggaa ccctatgaca gctgtaaaga ctcagataaa
    30661 ggtgagcccc aaaaaacgtg gctaatgtgg gatgccgtgg gggtatcggt aggcattgaa
    30721 aagattgagc tcagcgccgg cgaaagcctg cctgtacgga cacatccggt cactggcgtg
    30781 gagaccactt ttatccaagc tgacgctctg attctgtgct tgcaaggctt ggctctaagg
    30841 gccaactcct atctctgcaa ctccggacta gttcactaca cagatcaaga aaagaccatc
    30901 cacatagact tcgacaacgc gctcattgca cgtacttacg attcgctctt cgagctacga
    30961 aagactctca tggcctcaaa aacaccacca ccaacctcag atgagaacag gtgtacccag
    31021 tgtccatacg cagaaatctg catgccagat gaaatagcat tcatgagcac ggctgtttct
    31081 ggcaacctag caactgatga agatgcagtc gataatgccg aggtccgacg actggtgcca
    31141 gcgcgcgatg acagaatacc gctctatgtc cagactcaag gcgcagtagt ttcaaagcgc
    31201 gactgataga tctgtctcaa gtctgcatat atggaaacgt gcaactcacg acacaatcca
    31261 tacgcgcatt ttgcgcaaga gacataccca tctgctattt ttcccgtgga ggctggttct
    31321 acggaattac caagtccacc atgggcagga acgtgacagt tcgtagagag caatacaagg
    31381 ccactgctga cccgaaccgc gccctttcca tatcaaagcg atttgttgca gggaaaatca
    31441 aaaactgtag aacactgctc cgtcgcaact gccccgactg cgatacaggc gtcctagaca
    31501 agatgtcgga gttatgtcga caaagctatg tctgccaaca acatggccac tctcttggga
    31561 attgaaggag cagccgctag cctgtatttt ggaaactttg ggaccctgct cagcagcact
    31621 accggattcg atttcacagc tcggaaccga cgtcctccta aagatcctgt gaatgcagtt
    31681 ctttcatatc tatacagcat gctggtaaaa gacattacca tcgtcgcctt ggcaacaggg
    31741 ctggatccgt atgccggatt ctgtcatcaa ccacttcaag gaaggccagc tttggccctc
    31801 gacctcatgg aggaattccg gcctctagtt tgcgactcca ccgccatcag gcttatcaac
    31861 acgtctgcgc tcaagaactc tgacttttcg cgattgggca atggcgttat catgtccgac
    31921 aacgcaagac gcaaagtaat ctctgcctac gaaaagcgca tggataccct ggtaacgcat
    31981 ccgatattcg gctacgccat gagctatcgc agagttcttt cggttcaggc gaggctagta
    32041 gcacggtggt tgctgggcga aatcagcgat tatccaccat tttgtacaag gtgatgatat
    32101 gaggcgcagg tttatcgtct gctacgatat ttcagatcca aaacgcctgc gaaagatgta
    32161 tcggacaatg cgcgggtttg gggatccatt acagaaatcg gtcttcacgt gcgatctgtc
    32221 atccaaagag cgggcccaca tggtaaaagc aatccctaga atcattgatt gccaggaaga
    32281 ctccgtactg ataatcgata ccggcggcct tgacgccaag cgggaatccc tcgagtatat
    32341 aggaaaggcc ataacgccag acgaaggcaa ggcagcaatc atcgtctgat acagcccgaa
    32401 ttgaggtaat ccaaggtgat aagagaagcc aagactgcgg gtcatgataa catgttgtag
    32461 aagaaatcca agattcaagg ggcttccttt caaacccgct tcattgccaa caactcgagc
    32521 ccgagatgaa gcccggcgat gctctccgcc acggctacca tagccctgca acactaagaa
    32581 ccgctatgaa caagcaacct cagactgcag acaaaacatc agtggattcc gcttcagcga
    32641 cgctcaccag gttacctgca gattctatgg cgagcacgat ctctcgataa attcctggga
    32701 ttctcgagtc cgcactgagt gactcccaac aagtttttgc gctatcggtt agcctgccat
    32761 atgatgtctg ctaggcgtag acacgtcttt gcacctatgt cgcagtgcgg taattggttc
    32821 gcatttgtgc atcgacaagg tacacatctt ggccctagcc ataaacgctc gcagcaccca
    32881 gccgcaccgg gatatgatca gtcagggtag atggcgtgat cgaaatcccg gttcgacgct
    32941 tacgggcaac catcttcttt acaacgacca acgggtgtct aacttttaga ctggtctgca
    33001 aaacctgctc gcccccgcgg cgaaccttgg ccggctccac gtcccacggc gcttcccccg
    33061 gattctgccg aaggccaaat ctacaactaa agaccttagc aatccccgac taaattccag
    33121 acagcggcag cacaaactag aggatcgcgc agagatttgt tgcggtctac ttagacattg
    33181 tccccctcct aatcaggctg agatgctttt tgcaacctac ttggccgcag gtcacagata
    33241 ttgcgctata tcatgaccat gatatccctc tgaccccgac ggatatcggg gttcgtgggc
    33301 acatgtaacc ttggctaata gggtccatgc gggaaacggc cgggatatct cggattcccc
    33361 gggaaagctc gtcgccttgc gcaacttgat tgggcaggtg atgttccgca agccaacaga
    33421 gtactgcatc ctaaattccc ctagatgttg agcaagcctt cgcggacaaa ttcacgcgca
    33481 cgcatagcaa tgtagccaac ctaggtaaat ggcctatgaa ttacatgctg cacatgatga
    33541 tagggaggag ccacagcact accgtctccg aacatgccag atgatgcatg acaacctcaa
    33601 agcgtgtttg tacgctcgga aaggcattaa actcataaag accacggcca gcgttaaaca
    33661 tacatcaact acgctgcacg gcgggacacg atccagtact gagtggaatg caagtacgcc
    33721 cctgctgatt aagacttctt tggtaatgcc atttccttag aacttccctc taacagcacc
    33781 cagcatgcgg ttctcccgtc ccgggttccc taatcgctgg cgctatgccg gctcattcgc
    33841 ccacagcttt tttcaacaat ttgggcgtga ataaaggctt gaagcggaac ctcctgccag
    33901 ccccagctgg cagcgcccca tccatgctcc gcctgcatat attggcctgt cggagggatc
    33961 tttgtaatgc ctgttcccaa tgtccgctct ccccaagaca gcatcgccaa gactgcgggc
    34021 aaagtcacaa agcgcctgct taccctcatt gttctggcct tgcttgcgta tcatttcacc
    34081 gggtaccccg caatccgccg cccccggccc gaccggacgc tctctccgtg gctggccgag
    34141 cggcccatcg aatcattgct tgtaattgca ccccaccctg acgacgaaac cctgggatgt
    34201 gcaggcgtca tcatgtacgc tctggatcag ggcgcgcgcg tttgcgtcgc gctcatcacc
    34261 aacggcgacg ggtcttctgt gcccgcacac accagcggca aaggaccgct ccttgcccct
    34321 tcgcgctaca tatcactggg caatcggagg caaaatgaaa cccttgccgc gctgacatca
    34381 ctcggccttg aacctgagga cgtctgtttc ctgggctatc ctgatggcgg cgtgtcggca
    34441 ctatggtcag atttctggga ctattctgac ctatttagat ctcagtacac caaggcgaac
    34501 atgagtcctt actccaacag cttcaccccg aacgcccctt actgcggact ttctgtcgtc
    34561 aacgatatat gcagcatcat cagaacattc aatccatctc atatactcct cccccacccc
    34621 aacgacaccc acccagacca ttgggggacc ttcgccctgg tcacctacgc attgcaaagg
    34681 ctcgtatgcg agcaggagct ccaaaatcct cctgaaatgc ttgcgtactt aatccaccgc
    34741 ggagactggc cagtcccgcg gctgtatagc ccgcgctggc ccctagatcc ccccaacaca
    34801 gtcttgcgtt tgcgcgctgc tgatgaatgg gcataccagc cgctgaccaa tgaagaccgc
    34861 gcgcagaaac gtcaagccat cctcgaacac aaaagccaaa ccgctatgat gcgtatgtac
    34921 atgctgagct tcgtgcggag caacgaactc tttgccgtcc tacgctctcc tgtgatagca
    34981 cagctcccat cgcaccaaag cagatcctct gcttcccgcg actcgcactg gcagaccgcc
    35041 gaacccacca tctgggatcc acaaggcgat accctaacgc gtctcgtaag cggaggcgca
    35101 gatcttcagg ccgtatatgc ggccaccggc aacggcatgc tatacatcat ggcaaaaccc
    35161 aaaggacgcc cttcagacaa cgtccgatac gttctcgaca tatccgactt cggcaacccc
    35221 cgcacacaga tctcagtatc atacgggcca aagcagtctg tgcgaacctc cagctcgagc
    35281 aatctcaacc ccgacgacgt gcaaattcgg cgcactgtcg acggcattga gttcgcgatt
    35341 ccactcacac tctttcagac cgacagagga acaggcacaa ccgcccccag cgccgtcctc
    35401 ctggccatac actccgcgat gattggagat atcgtcgtag acagaacggg ctggacagtc
    35461 ctgagactct cagaggacca acccgacata ttccgatgag tcacgtccga tatgatgctg
    35521 taaaaaggcc gtgtccctgt tgtagtgggt atggaaaggc cactagttct ctattggaat
    35581 tggaggagaa ccaatggcta catatcagat gagcatatcc aaagagcaat tgcatggact
    35641 ttttgaagca ttccttaatc aggtgctcaa actgca
//
LOCUS       DAOSYL010000018        34697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1022_length_34697_cov_7.103170, whole genome shotgun sequence.
ACCESSION   DAOSYL010000018 DAOSYL010000000
VERSION     DAOSYL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 34697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34697
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1022_length_34697_cov_7.103170"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1046)
                     /locus_tag="PLU15_04700"
     CDS             complement(18..1046)
                     /locus_tag="PLU15_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HOO30252.1"
                     /translation="MREKIRVIQYGCGKMGKYFLRYLYEKGAEIVGAIDLDPALEGKD
                     VGEVAGLGFKINVPISSNAEKVFGECDADVCVIATRSLMPDVYDAFALAAKHGVNAIS
                     TCEEAFYPWNTSPALTNRLDRLAKDNNCTLAGSGYQDVFWGNLITTLAGSSHRIERIE
                     GVSSYNVEDYGIALAEVHGAGLSLEEFDKQIAQSDSLPSFMWNANEWLCSQFGWSIKS
                     TEQKLVPTTHDKDIKSETLGKVIPAGHATGMSAIVTTVTNQGPIIISECIGKVYAEGE
                     FDRNDWSIIGEPNTVVKIARPATVELTCATVVNRIPQLLQAPPGYYTTDKMPPARYIS
                     YPLHFYVK"
     gene            complement(1147..1989)
                     /locus_tag="PLU15_04705"
     CDS             complement(1147..1989)
                     /locus_tag="PLU15_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO30253.1"
                     /translation="MDLLPIGKMAALNHTSVQTLRYYDKIDLLKPLYVNQDTNYRYYD
                     IKQSAILDMIGYMKSLGMSLEQIKEQFDKEDIEAIKDILERQSKNIDEEIRKLHHMKK
                     GVEACIRNYDRYLQMPQEGEITFEFIPDRRIFCYDGKVNIYDHGLDNYEYILRELKKQ
                     VIIEDLPMVYFCNVGSILRKEMIDAGRFVSSEIFVFVDNDFEEQEGIEIIPAGDYVCI
                     HFHSFWNEKANARKLFHFIHENGYEIVGDYICEVVAELPIFDNNERNMFVKLQIPIQA
                     PRDG"
     gene            complement(2245..2376)
                     /locus_tag="PLU15_04710"
     CDS             complement(2245..2376)
                     /locus_tag="PLU15_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30254.1"
                     /translation="MYFEEWERAESTGASLSVINRASGEIETDWYSRLTGVELEQAR"
     gene            3005..3700
                     /gene="vanR"
                     /locus_tag="PLU15_04715"
     CDS             3005..3700
                     /gene="vanR"
                     /locus_tag="PLU15_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HOO30255.1"
                     /translation="MNDRILVVDDEKAIADLIELYLRNENYIVFKSHNGQDALALVES
                     EPLDLAILDVMLPDMTGFEICQKIREKHNFPIIMLTAKDEEIDKITGLTLGADDYITK
                     PFRPLEMVARVKAQLRRYTRYNPSEPADEENVIAFSGLVIDKDAHECTLNERPLSLTP
                     TEFDILWMLASNRGRVVSSEELFREVWGEKYFTSNNTVMVHIRHLREKMNDSAENPKY
                     IKTVWGVGYKIEK"
     gene            3678..4832
                     /locus_tag="PLU15_04720"
     CDS             3678..4832
                     /locus_tag="PLU15_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO30256.1"
                     /translation="MATRLRNRKRDKGDYSRLKKKVFRRMLLIVLSAVAVVFFLCLLT
                     MWRFRPNVVNFLEKAFHLSNDEAGRIWQEVFSDNALLYVFIAIIVFILIFLRFSLSWF
                     AKYFDEISAGIDQLVDKPGSDIILSPELAHMETKLNKIKGTLERQKWDAQEAEQRKND
                     LVVYLAHDIKTPLTSVIGYLSLLDEAPDMPPEQRAKYVGITLEKAYRLEQLIDEFFEI
                     TRFNLQSIVLNKGKINLSFMLNQVADEFYPMLEPEGKKAVVDAPDGLTLWGDADKLAR
                     VFNNILKNAAAYSYRGSIIRISARQEDQSVIIVFANQGDPIPSAKLETIFDQFFRLDA
                     SRSTNSGGAGLGLAIAKKIVTAHGGNITATSNEERTVFTVTLPNQDGTVG"
     assembly_gap    5208..5307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5425..6474
                     /gene="vanG"
                     /locus_tag="PLU15_04725"
     CDS             5425..6474
                     /gene="vanG"
                     /locus_tag="PLU15_04725"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HOO30257.1"
                     /translation="MKRTRVAVIFGGSSTEYEISLQSAYSVITNLNPEEYDLILLGIT
                     RQGAWMRFTGPVEQIKDNTWMDHSVSVPAIISPDRNTHGILEFHGNKVTTTRIDVAFP
                     VLHGKNGEDGTVQGLLEMAGIPCVGCGVLSSAMCMDKDIAHRIVSLAGVKTPPSIVLH
                     SYVSEAELLKRIAHLKYPLFVKPANAGSSFGITKVSQESGLACAVSAAFEHDRKVVIE
                     ESIDGFEVGCAVLGNDVLTIGEVDEIELCNGFFDYTEKYTLKTSKIHMPARIDVETAA
                     RIKQTAIVIYRALECSGYARVDMFLTPEEDIVFNEVNTIPGFTTHSRYPNMLKGIGMS
                     FEQIVDTLIRLAIDR"
     gene            6471..7235
                     /locus_tag="PLU15_04730"
     CDS             6471..7235
                     /locus_tag="PLU15_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOO30258.1"
                     /translation="MRTLKLYAEDIGKGNLILVNPSHPLATKPADEVLVPIRPAFPEI
                     VLERQTARMLAEVAALLQCEDQIVPVSGYRTMQEQQAIYADSMRENGKEFTKKYVATV
                     GCSEHQTGLAIDLGENRPDIDFIRPYFPYTGICQEFRKLSVQYGFIQRYPAGRESITQ
                     IAHEPWHFRYVGYPHSQLIRGNEFTLEEYTDCLKHFPFDGAHLRIKHDNYDFEIFHVP
                     VEPGQEVEIELPDRIPFQVSGNNDDGFVVTLWRHLG"
     gene            7232..>9369
                     /gene="vanT"
                     /locus_tag="PLU15_04735"
     CDS             7232..>9369
                     /gene="vanT"
                     /locus_tag="PLU15_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HOO30259.1"
                     /translation="MNLQEEFPGIDYFRIIAAFLVVANHTSPLSSVNGTADFILTRII
                     ARVAVPFFFMTSGFFLFRGKATDSEADRARLFRFLRKTAALYAISTLLYLPVNIYAGT
                     FRTEHLAKELLKDIVFDGTFYHLWYLPAAMIGATIAWLLLKRLGPGHAFGIAFILYLV
                     GLFGDSYYGIAERVPFLQSFYASVFTVSDYTRNGLFFAPVFLLLGAMMAPPARRLQPK
                     ACFTGLAVAFLLMLAEALLLHTCGIPRHDSMYLTLLPCAALLFQSLLHINGKTRKELR
                     DISTLIYLVHPMMIILVRWLAKAAGLKRWLIANSVIHFLAVAVSSLAAAEAILLLLRV
                     FQNRELAPEHATMDRAWAEIDLTCLSHNVQVLRKAMPDGCKLMAAVKGNAYGHGDVEV
                     AKHLNQIGIDAFAVAAIDEGIRLRKHGIRGTILILGYTVPERAGELARYRLSQTVVDR
                     EHAVRLNACGKPIHVHIKVDTGMHRMGEPWDHVSEIASIFGLKNLYVDGIYTHLSVSD
                     SMEPEDAQFTEMQIDRFYHLLETLREQNVTLPKIHVQSSYGLLNYPMLKCDYARVGIA
                     LYGTLSSPHDQTRLHLDIKNVLSLKARVSLVRTVTAGESVGYGRHFIAERDTCVAILP
                     IGYGDGIPRNLSGGRGSVLIHGCRAPIIGRICMDQLMVDVTGIPDVKRGDVATLIGRD
                     GSAEITAEQAAADAGTITNELLSRIGIRVK"
     assembly_gap    9370..9469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9667..10743)
                     /locus_tag="PLU15_04740"
     CDS             complement(9667..10743)
                     /locus_tag="PLU15_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30260.1"
                     /translation="MAYPCAADSTLLEISGLQTIYPTARGEVQAVRNVSFSVSQGEIV
                     GVVGESGCGKSALLLSIMGLLPSPGRIVGGRVMFGGTDLVDLSEAEMRTIRGKEIAII
                     FQDPMTALNPAYRVGEQIAESLRVHGMVPVSAGDTGRLCGDGGGLGPRMRKTRSRKLS
                     AAEEREYVFELMQSVGIPMPEQRHLDYPHQFSGGMQQRIMIAIALACKPKLLLADEPT
                     TALDVTIQAQVLDLIDEINETTGTAVVLVTHDLAVAADFCDSIAVMYAGQIVERGPTE
                     AVMEGSAHPYTRGLLRSMPVLGERKPLRPIMGEVPDLANLGPGCSFAPRCDFSEDVCF
                     ERAPDVSIVEKGHEVRCHHHSGAL"
     gene            complement(10750..11676)
                     /locus_tag="PLU15_04745"
     CDS             complement(10750..11676)
                     /locus_tag="PLU15_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30261.1"
                     /translation="MGHVSIPSDELCKGVISTARTVPYVRASRRRGTVKRLLAHGGGR
                     TGLIIVAAFVLVAVLAPVIAPYAPDEVDLDSWLIPPGFGSGHLLGTDQLGRDILSRII
                     YGSRVSILVGLTTILISSVVGIGLGMLAGYYGGALDRVLARFTELLLAFPYLIFVIAI
                     MSVIGPGFWNLVWALSFKGWVEFYRLARALTMSEKNREYVDAARALGRSDGVILVREI
                     LPNISNSMLVLGTLRMGNMIMTESSLSFLGLGIPPRIPAWGSMIADGRRYMLGAWWVA
                     TLPGIALALLVLGMNLLGEGLRDVLDPKMMEG"
     gene            complement(11689..12714)
                     /locus_tag="PLU15_04750"
     CDS             complement(11689..12714)
                     /locus_tag="PLU15_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30262.1"
                     /translation="MIRLSRTQAKIVERILATIPLALGVAVAAFIFMRAIPGDPVDIM
                     MGEAAWTSEEEMAALRGEMGLDEPMHVQLWTYLKQVFKWDLGYSIVRSRPVADIIREA
                     LPATIELAVFAMLFALAVGIPIGVASALNPNSFMDRVGTCLSLVGVSLPAFWLGIVGI
                     LIFSMILGWLPTSGRIAYGAQPAHVTGLLVLDALITGNLPALGNALKHICLPMITLGL
                     GVAAVVARVTRSSMLDVLSADYVQFARAKGVPERDVTCRHALRNAMIPTVTLVGLQFG
                     SLLGGNMIVETIFGWPGLGRVVVDAIFARDYPVVQASVMVYALTFVLINLVADIIHTV
                     LDPKISL"
     gene            complement(12711..14264)
                     /locus_tag="PLU15_04755"
     CDS             complement(12711..14264)
                     /locus_tag="PLU15_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30263.1"
                     /translation="MKLRTILTTLIIAMILAFGFLPTTTGQTRKQLSVVIDTDSTRAD
                     EAQAIAHNLRAIGVSADVRIWEWNALKERIQAGERQMYLTAWGSAYFDPFDIIVPKFY
                     SGDRGNYSGYSNARFDAALDRALRAVDNDARREAYFDAQQILYDDAPAIFGYSLMEVE
                     AARSDVLNWQPSMDSRVNLHDVGLARGDTIVVGLYADKILTLDPAAYRDRPTETVIRN
                     MFDALVTRTWDGKVVPEIAEAWKAVSDTEYLFRIRRGIKFHNGDLLDADDVVFTFDRV
                     LREGAMGGRTSPRKGLLGPVEKVEKVDQYTVRFVLSSPFPVLPQALVHFQIVPRKYVE
                     RVGDEAFARKPVGCGPFKYVEGQFDDKIVMERFDDYYGGSPSIPPVGPARVKRAIFRM
                     MPDPTVRVAAVKTGEVHIIQSVPSHMAAALVRDKNIQVKSVEGTRVYMVEMNCRRPPF
                     DDPRVRRAMNYAVDWDAILRTVYAGNATRLATAFLPSGFGFNQSIVPYKYDPARAKAL
                     LAEAGYSTK"
     regulatory      complement(14465..14609)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            14822..15943
                     /locus_tag="PLU15_04760"
     CDS             14822..15943
                     /locus_tag="PLU15_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO30264.1"
                     /translation="MTAQTCRKPLVSAVGVSKHFVMSESLLSRRLTGLSARQVMAVDA
                     VSMEIYPGETVGLVGESGCGKSTFGRVMVGLLQPTSGQVYYGNTPIWGQKSIDRSELK
                     HRAQIIFQNPYSSLNPRYTVRQTIDVALAARGVPPRHRYEEARSLMTRVGLSPDYLER
                     YPHQLSGGQRQRVAIARALATQPEFIVADEPLSSLDVSVQAQIISLLEDLQNEYGLSY
                     LLIAHDLRVVYHMCNRVAVMYGGQIVEFAPTDALYERPLHPFTRQLLGAIPGTGGRRR
                     RNRNGRGHSPAQASFATSIEAESMIDSSQAHIANACQFWPRCEQAAPVCKKSSPELLS
                     LSFGSASAFGASHSDTASGASTEPQDDHLVRCHMAHNRQ"
     gene            15963..16733
                     /locus_tag="PLU15_04765"
     CDS             15963..16733
                     /locus_tag="PLU15_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30265.1"
                     /translation="MKLFSANKSFLGRYDRMLAAYAVTLLVMMCGLVGFSAATGAAGN
                     GVDEAQMVLDNMLRAQENLTSFELPATARVYLGGRNVLTANVQVLASRPNNLAVKWLG
                     VTIRPRKGIIFIDPAQFVSENYTLSVLARPSTARQPSVTPRFEDGHSNDGWVISAISR
                     PGTASPLRWKLYVDPETWLIVQAEVITADSDTCIIKAKYRNAGYRRWEPLFVHAEGKM
                     VLEEFLPDVLVGLIMGQAAREKSAYVDLDFSDGRSKKA"
     gene            complement(16851..17123)
                     /locus_tag="PLU15_04770"
     CDS             complement(16851..17123)
                     /locus_tag="PLU15_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOO30266.1"
                     /translation="MTKSDLVDSVAVKTGMTKKDTARTVDAILETIKEQLKAGEKVQL
                     VGFGSFEVKKREARVGRNPKTMEEIRIPARNVPVFRAGKELKESVL"
     assembly_gap    17238..17337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17338..18812)
                     /gene="spoIVA"
                     /locus_tag="PLU15_04775"
     CDS             complement(<17338..18812)
                     /gene="spoIVA"
                     /locus_tag="PLU15_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOO30267.1"
                     /translation="MEKFDIFRHIAERTGGDVYIGVVGPVRTGKSTFISRFMDLIVLP
                     NMKDAYEKERTRDELPQSGAGKTIMTTEPKFVPGEAVEITLGENIKVAVRMVDCVGYT
                     VPGALGYEEADAPRMVRTPWYEEEIPFQRAAEIGTEKVIQEHSTIGLVVITDGSITEI
                     GREAYIDAERRVIAELKELGKPFIVVLNSTHPEAESTRTLGEALARSYDVPVIPVDCL
                     NMSTADIMQVLGGVLYEFPIREISVKMPEWVVELEDSHPVRTGFVSIVHEAVSAIDKV
                     RDVSEAVRILGTAKNAESATVTLLDMGTGRVWIEVAAPKSLFYAVISEMSGLEVAGDS
                     DVIRMMRDLTHAKREYDKVAVALEQVRTTGYGIVAPFLDEIYFEEPDIIRQGNRCGVR
                     LKAMAPSIHMIRADIRTEVTPLMGTEKQSEEFAEQIAEDFAENPSKIWETEFLGRSMQ
                     DLIREGIEAKLAHMPIHAQEKLQETLQKIVNEGSGGLICIVL"
     gene            complement(18937..19788)
                     /locus_tag="PLU15_04780"
     CDS             complement(18937..19788)
                     /locus_tag="PLU15_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOO30268.1"
                     /translation="MHSFPRGAVVSNSSGTCIAIEKDMASQFVGVIPEPEEAVSRIAR
                     NLQLVYGIGPVREAGLKEEGYATLFDLLSHEKWGQGARPIVEALERADGWELLRYGVP
                     GLDILAFCAPEEVVFLDIETTGLSSTSALFMVGMIIPSERGLILHQLFAREYEEEPAV
                     IEETLARLADARAVVTYNGKSFDMPFLLRRLAYYGREDNPHAAIVDLCPPARRKYSFS
                     LPNCRLSTIQTMVLDVPRDGDIPGSLIPGLYNDWVRTGDAEKIIPVLDHNELDLVAMV
                     DLLPLLI"
     gene            complement(19803..21035)
                     /locus_tag="PLU15_04785"
     CDS             complement(19803..21035)
                     /locus_tag="PLU15_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30269.1"
                     /translation="MLPAKKLRTSLCAIAFLCIMAVSLAAPHGADAVWAAETVRSLLE
                     LPSADRALGNNDGATLDCLVIQESGLAVATHVVEVDLVAGMNKVRVAGVSDTIQCGAA
                     EIISGTVPFTVVSAATNRAAGELIFSVKAETASHAVFRVAYPLSGLTWSASYNAVLEP
                     TKDAAGIWGWYSIHNETDCDLAPARIVLMGGSTNPLGSKPGFKGDSALGKTVATIDSP
                     EMIPAGSQRNIRFAGGAAVPARLVYVVDWGPMAILDSSSQGKGERPVMLGLEFSASKE
                     TGLAYPLPGGRISVFSLSSDGTTFFLGEDLVSPCQSGTTLIAKVGPAVLLTAERTRTD
                     QRKVGTSSWEEAYQVRIANAGTASVTVINVEEFPGQWTILQSNIPTPPIVSGRAQFSV
                     NVPAGGQANILFRVRYTL"
     gene            complement(21090..22370)
                     /locus_tag="PLU15_04790"
     CDS             complement(21090..22370)
                     /locus_tag="PLU15_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOO30270.1"
                     /translation="MDRSEVAQILGVDDDIMELASCVHEEISDEIATVNSICRINTAR
                     VLEALAKAGLSDYHFGGTTGYGYDDIGRDCLEEAFAHVFGAESALVRIQIVSGTHALA
                     AVIFGVLRPGDLLVSAFGTPYDTMVTMIGRDRPVRGSLAEYGIDYLEVQPAADGGPDF
                     EQIASASRGARMVMVQRSPGYSWRKSLGIDEIGRIARCVKEANPKAIVFVDNCYGEFV
                     ETREPTAVGADVAAGSLIKNPGGGIAPCGGYIAGRADIVEMAAERLTAPGLGCECGPS
                     LGYTRQLAQGLFMSPLIVGEAVAGSIFAAAFLSKLGYEVSPCPGQARHDIVLAVRLGS
                     ADVVSSFCQAVQSASPVDAAVRPIAAPMPGYADEVIMAAGAFVQGSSIELSADAPLRP
                     PYDAYLQGGLIRHQVEFAMLKFAQDVKEKARSRE"
     gene            complement(22357..23436)
                     /gene="miaA"
                     /locus_tag="PLU15_04795"
     CDS             complement(22357..23436)
                     /gene="miaA"
                     /locus_tag="PLU15_04795"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOO30271.1"
                     /translation="MKESDGTDRVREAPRGESVGDGIGRVGNGAGHPGERDFQRRPPL
                     LVIVGPTAVGKSAVGMELARKLGGEIVSADSMQVYRGMDIGTAKPSQAERAEIPHHLI
                     DVADPDEIFCVARYQEMAREAIRDIAARGRLPIMVGGTGLYVDAVVRGFLFPDEGRNP
                     DIRRRLEGEAEVTGSPALHVRLAEVDPVAAERIHPNDLRRIVRALEVYEVTGMAISEL
                     QRKHEGESEFDTVIYGLRMARQALNKRIDDRVDIMVEQGLIDEVRSLMEHGYDPRYTA
                     VQAIGYKEFALYLAGEESLEEAIDTLKRETRKYAKRQMTWFRRNPNIRWIDVECGRGP
                     EDIADEIAASIESWLKGVGFSVGSK"
     gene            complement(23414..24259)
                     /locus_tag="PLU15_04800"
     CDS             complement(23414..24259)
                     /locus_tag="PLU15_04800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO30272.1"
                     /translation="MKDVGRIAVTTSARGGRHQVDLGIRLAHELGAPFITRAGRSLQS
                     LADAYSEDGRGELEAFMVVEADRISVVRLADAGRRSAELFFHPGMALSRIRRLKQGGV
                     DQMVEAMGISSGDHVLDCTIGLASDAIVSSWVVGSRGRVVGVEVSPVLSIITREGLTS
                     YTEADPDVLDAMRHIEVVASDHLDYLRSCADKSFDVVSFDPMFREPVTSSAGIAPIRP
                     WACHTPLSVEALSEACRVARRRVVVKDRADGDELDKLGVGHKIGAKKSRQEYGVIFVE
                     GERWD"
     gene            complement(24611..26395)
                     /gene="mutL"
                     /locus_tag="PLU15_04805"
     CDS             complement(24611..26395)
                     /gene="mutL"
                     /locus_tag="PLU15_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOO30273.1"
                     /translation="MRIIRLAPEVANKIAAGEVVERPASVVKELVENSIDAGANTISI
                     ETLGGGLELVMVTDDGCGMGREDAARAFERHATSKISRAEDLFSIESLGFRGEALPSI
                     ASVARVRLVTCERGSGEGTVVVAEGGRVGEPTAIGAPCGTSIAVRDLFFNTPARLKYL
                     RSVSTENRRIIGTVGSLALAHPEIGFSLKVDGRTALVTPQGGSIEEAAAAVLGLEVAR
                     EMIPVKSSSHGIFVKGLISLPQHAKGKRRDSQYIFVNGRPVVSTTVWSAIDKAYDKTV
                     PVGIRPPVILSIEIDPSQIDVNVHPAKSEVRFASNSDVFSAVCGALAFVLRSVDAVPD
                     LEPVQPTAGWGQTGAVSRQWRGPGASYGGEPQHLAISSWDRESAFREVRPASGPGVAL
                     GLAPARSGLAGAVPEVIGQLDDTYILAQGAEGLVIIDQHVAHERILVDKYREEFAKEK
                     PSVQLLLTPEIAEIGASDVEIVAKHGDVLSRMGYELDLFGATSVVIRGVPSQSAGVPP
                     AESLMAAIEGIVSAPSGSDPGLLADHIAVSLACRGAVKAGDSLTIAEMKSLVERLFES
                     EDPYRCPHGRPVVVLVPGDALARSFGRK"
     gene            complement(26401..29049)
                     /gene="mutS"
                     /locus_tag="PLU15_04810"
     CDS             complement(26401..29049)
                     /gene="mutS"
                     /locus_tag="PLU15_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO30274.1"
                     /translation="MSKPTPMIRQYRELKKRCPDAILMFRLGDFYEMFMEDAEIASRI
                     LGIMLTSREVGSGNKMPMCGVPHHAVDQYIATLVQNGHKVAIADQVEDPRTAKGLVRR
                     DIVRVVTPGTLADQVDPGRNNFLIAVVATESGVGLASCDASTGELAATQADGDDAFAL
                     ISEEIDRIAPAECIYPGDSRGLQGSSGPRFDEDDKGGGCPVEGSFVAYPRQPMDFERG
                     RAEETLMSQFGVTSLEGYGAAGMDLAVRAAGALVSYLQETQMTSVPQISRMRVYHLGE
                     FMRMDTPTRRALEIFHRERGLLSVLDSTVTPMGGRLIRSWFERPAVSAAEIESRLDAV
                     EELAGDLFLRADVRKTLDRMYDLERTCGRIGYRTAGPRDLLALASSLEAVDDLAAVLG
                     EAKSSRIIDLAGALDPVPEATERIRASIKLDAKASANEGGVICEGYSEEVDELRRAST
                     EGKEWLIRLEARERERTGIKSLKVGFNQVFGYYIEVTHANSHLVPDDYTRKQTLANSE
                     RYITPELKELESKILGAEERLIRLEAAIFEQVRDDISAYLPRILATAQAVAELDVLAS
                     FAEDARNRGYVRPQINTGKATVIRDARHPVLEEMLGSARYVPNDIEASCNGGRLLIIT
                     GPNMAGKSTVLATIGLITVMAQVGSFVPAADAEIGICDAIYYRTGSYDDISAGKSSFM
                     VELLEIANILNNATDRSLILVDELGRGTSTYDGMALAWAVAEWVHDHVGARALFTTHY
                     HELAALEERLPYARNYHVGAVERGGELTFLYRLARGSVDKSYGLHVAKMAGLPGEVIR
                     RAQQILKGLEAASPSEGHQMTLFGWVDEEPVRGSAAEVIAEDVSLAQTAQRVLGRLEG
                     VDADNLTPKQALDLVYELKDLARMKE"
     gene            complement(29042..30514)
                     /gene="miaB"
                     /locus_tag="PLU15_04815"
     CDS             complement(29042..30514)
                     /gene="miaB"
                     /locus_tag="PLU15_04815"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOO30275.1"
                     /translation="MEVRRRSAKVVTFGCQMNERDSETIMGILSSLGFEESSRVEDAD
                     IVIFVTCCVRENAENRAFGNLGEMKRLKEGNPNLVVGMCGCMAQRSDLREIVRARMPW
                     VNLVFGTHNIHRLPELLERVFAGERVVEVWDEPDEVVEGLPAVRAYGHKAFVNITYGC
                     DNFCTYCIVPYVRGRERSRRMEDVVAEVCDLAGSGCVEVTLLGQNVNSYGRGLPPLPS
                     GSSPDFADLLHEVNLVDGIRRIRFTTSHPRDFSDKMIEAVASSEKVCEHFHLPLQAGS
                     DRVLEMMHRGYTAERYLALLDRIREAVPGASITTDIMVGFPGETEEDFQATMDVVRQA
                     RFDAAFTFIYSPRPGTVAARMPDQIEDEVKHDRVYRLIDLVNSIASEVNDELCGSIVE
                     VMVEGPSERDPSVLSGRTRTNKMIHWTSEKAHNPGDIVMLRVSQTRTWTLYGEEIEVR
                     SGHRGTLTCDGHEAAGEEMQGRTARSSGLATDGRCDCNDV"
     gene            complement(30548..31765)
                     /locus_tag="PLU15_04820"
     CDS             complement(30548..31765)
                     /locus_tag="PLU15_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO30276.1"
                     /translation="MGASLGPGIVIVGLGPAAVNALEGIRSQDVDVPVTILSQEPYYA
                     YYRPRLSHLLGEEVSSESLAIKKPQWYEERNAQVLLGQTAKAIDVDRKTVSLDNGSEL
                     SASSVILACGSSPFVPPLSGLDRENVFSLRTVDDARAISSACETAKTVCVVGAGPLGL
                     EAAWALAKRDLEVHLLEHGSRLLSKMLDQNASRMLAEIVESAGIVLHLSAFSEALFPG
                     GIRLAGGEEIPGEVILFSTGVRPNLDVPRSAGLKINRGVEVDKLMRTSTPWIYAAGDV
                     AEFEGSVGGILPVAVAQGKVAGVNAAGGQEEYVAVPPSYTLMVMGTRLFSVGDVQDGP
                     DVTSIVDVDYDSRVYRKLVLSGDKVVGGILFGDIAGSDKIRRAVEEGVQLPGAAKESV
                     PDYASLLDMIVRD"
     gene            complement(32599..33096)
                     /locus_tag="PLU15_04825"
     CDS             complement(32599..33096)
                     /locus_tag="PLU15_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30277.1"
                     /translation="MRIPTDPDSIGLIKAMRPFLSERGQAAVDDVLGSVNLVGIYTSV
                     SDVLTKRRGMGHDRPLAFLSSLANMELDPKIVAKAVDSMMEMSKKPESEPERATSPKG
                     PSGPSADDVAKMLQNVMAKASSDPNFAALLGSLAEEGMRSNMLPKLLESLGPEFLPPQ
                     DQPEQ"
     gene            complement(33093..34289)
                     /locus_tag="PLU15_04830"
     CDS             complement(33093..34289)
                     /locus_tag="PLU15_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOO30278.1"
                     /translation="MRRCQRLGGNLGVQSFRFSATGRPREQKIVVFDQNISRNQWAEI
                     LANCRGHILRELPLVNGVLVLVDEEEIADVQALAQSRSGVVRVEDDIDIELAYEVGKV
                     QWGPRFREDVPWGVKRINIGDASQTGNGVNVGILDTGVDSRHPDLAGNIKGGYSAIAG
                     IDSASDDNGHGTHVAGTIGALQNSIGVLGVAPRANIYAIKVLDAKGSGKLSSLIDGIG
                     WCVEKGIKVVNMSLSSSTENQTFRDMVQSARKAGITMVCAAGNRGPGSNSVGYPAKYV
                     ESIAVAAIDENNNIANFSSRGREISISAPGVDILSTWPGKQYRKSTGTSMAAPHVTGA
                     VALLLEADPSLSPADIKSLLQASADRLDGVSSDEQGAGVLNVSQALSRVSKKRVREVC
                     VMGVAT"
     gene            complement(34308..34475)
                     /locus_tag="PLU15_04835"
     CDS             complement(34308..34475)
                     /locus_tag="PLU15_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30279.1"
                     /translation="MEESLNREFIADILTIATIAVSLWLTGEKDDTVLEKKCETLPNM
                     GMFRPFGELSK"
BASE COUNT         7984 a        10294 c         8265 g         7854 t
ORIGIN      
        1 ttcttgcctg atctttacta cttaacgtag aaatgcaacg gataacttat gtaccgtgcc
       61 ggcggcattt tgtcggtcgt atagtatccc ggaggagctt gcaatagctg tggtatacgg
      121 ttcacaacag ttgcgcatgt cagttccaca gtggccggac gggcgatttt gactactgta
      181 tttggttctc caatgataga ccaatcgttc ctatcaaatt cgccttccgc atagactttc
      241 ccgatgcact cagagattat gataggccct tggttcgtta ccgtagtcac aatcgcagac
      301 atgcctgtgg catggcctgc aggaattacc ttgcctagcg tctctgactt gatatccttg
      361 tcgtgggttg ttggcaccag cttctgctca gtcgatttga tggaccatcc gaactgcgag
      421 cacagccact catttgcatt ccacataaag gacggcaagg agtcgctttg agcaatctgc
      481 ttatcgaatt cctcaaggct aagtccggct ccatggactt ccgccaaggc gattccgtaa
      541 tcttccacgt tatagctgga gacgccctct attctttcaa ttctgtggct agagcctgcc
      601 agtgtcgtga tcaggttgcc ccagaacaca tcctgatacc ctgatcccgc cagagtgcag
      661 ttgttgtcct tagccaatcg atccaaacga ttggtcaacg ctggtgaagt attccacgga
      721 tagaaggcct cttcgcaggt gctaattgcg ttaaccccgt gctttgcagc aagagcaaag
      781 gcatcataca catcaggcat caggcttcta gttgcgatta cgcacacatc cgcatcacat
      841 tcaccgaaaa ccttctcagc attcgaactg atcggcacgt tgatcttgaa cccgagtcct
      901 gcaacttcgc caacatcttt gccttcaagt gcagggtcga gatcgattgc gccaactatt
      961 tctgcgccct tctcatacag ataccgtaga aaatacttgc ccatcttacc gcaaccgtac
     1021 tggatgactc taatcttctc tctcactaca ccatccccct tgcgcttttt gataggacgt
     1081 tcgtgcgttg tcccttccct gttgttcgtc ttcgactcaa gtgtaatctc tattgtacga
     1141 atagagtcaa ccatctcttg gggcttgtat cggtatttgc agcttaacaa acatatttcg
     1201 ttcgttattg tcaaagatcg gtagttccgc gactacttca catatgtagt ctcccactat
     1261 ctcgtatccg ttttcatgta tgaaatgaaa cagctttcgg gcgttcgcct tttcgttcca
     1321 aaagctgtga aaatgtatgc acacgtaatc tcctgcaggt attatctcta tgccctcttg
     1381 ctcctcaaag tcgttgtcga caaacacgaa tatctctgaa gagacgaatc tgcctgcatc
     1441 aatcatctct tttcgcaata tagatcctac gttgcaaaag taaaccatgg gcagatcttc
     1501 gatgattacc tgtttcttta gctctctcag gatgtattcg taattgtcga gcccgtggtc
     1561 gtagatattt actttcccgt cgtagcagaa gattcttcta tccggtatga actcgaaggt
     1621 tatctcgccc tcctggggca tctgcaaata cctatcgtaa ttccgtatgc aagcctctac
     1681 gccttttttc atatgatgca gcttcctgat ctcctcatcg atgttcttgc tctgccgctc
     1741 gagaatatcc ttgatcgcct caatatcttc cttatcaaat tgctccttta tctgctccaa
     1801 ggacattccc aaagacttca tatacccaat catatctagt atggcgctct gcttgatatc
     1861 gtagtatctg tagtttgtat cctgattaac atataacggt ttcagcaaat cgatcttgtc
     1921 ataatacctc aaagtctgaa ctgaagtgtg attcaaagct gccatcttgc caatcggcag
     1981 gagatccacg tttttcacct caccacttaa gatattgaca tttccaaatg cttgcaagtg
     2041 ctcatgaatg cggatagcta cagtcaaacc aagcttcctc gccattgctg tgggatagca
     2101 gctaggtttc ccaaagcaag ggctctgcga ttcaaagaaa gaagctgtag gcactaacac
     2161 attggacatg aacttgctct agccatgaca actaatgcca taaggccctc ttctttgcga
     2221 atactacgtg cccctccccc attcttatct tgcctgctcc aactccactc ctgtgaggcg
     2281 gctgtaccag tccgtctcga tttccccaga agctctgttg atcactgaca agcttgcgcc
     2341 ggttgactca gccctctccc actcctcaaa atacaatctc cttcagagcc tccatctgtc
     2401 ttcgcttcgg taaggcaaca gcccttcttc gaatcatcag gttaattcat ggcactagag
     2461 acagcttcgc cattgtcagt ctgtcctgcg acccacaatc cgcagccgtc acacggatta
     2521 tccacggatc gagcatctca tctcggatcc aggatgtaga tgatgtcgta gggcacaggc
     2581 ccgtcaggaa aggtataatg aattgaggac gatcaaccac tccaagccta aaggtagggg
     2641 cttgcaagag cccccgctga cctttccgtt gcggtgttct tggggataat gcgttgggat
     2701 ttcggtcacg aaaagcacac ccgaatgttg gcgtaactca tgggtacatc accgagcagg
     2761 caaacacatg tgcaaggctt gggaaattcc atcgtacaga tgcaagtggc aactcaacgg
     2821 tagagccggg cacttgggat ctctcgaaac aagctaagaa ccgaatcagg caagtgcaca
     2881 aggagtagcc aacaaagcgt ccagatatgt tcaaaggaag ctgcaagcac agtgctgtgc
     2941 gaaaggaggg ggaacgctac ttcctcccca cacctgaagg ggaatctccg tagcgtgatg
     3001 tttgatgaat gaccgtatac tagttgttga tgatgagaaa gccatcgcag acttgattga
     3061 actgtactta agaaacgaga actacattgt tttcaagtcc cataacgggc aggatgcctt
     3121 agcccttgta gaaagtgaac cactggatct ggcaatcctg gacgtgatgc tccccgacat
     3181 gaccgggttt gagatctgcc aaaagatccg ggagaaacac aacttcccta tcatcatgct
     3241 cacggcgaag gatgaggaga tcgacaagat cacaggcctc acactgggcg cagacgatta
     3301 tattacaaaa ccattccgac cgctcgagat ggtggcccga gttaaggcgc agcttcgcag
     3361 gtacacaagg tacaatccct ctgaaccagc tgatgaggag aatgtgatcg cgttttcagg
     3421 cttggtcatt gacaaggatg cccatgaatg cacgctcaac gagaggccgt tatccctaac
     3481 cccgacagaa tttgacatcc tgtggatgtt ggcatccaat cggggccgcg tggtcagttc
     3541 cgaagagttg ttccgcgagg tatggggtga gaagtatttt actagtaaca acacggtcat
     3601 ggtgcacatc cgccatctga gggagaagat gaacgattct gcggagaatc ccaaatacat
     3661 aaagacggta tggggagttg gctacaagat tgagaaatag gaaaagggac aaaggcgact
     3721 actcaagact aaagaagaaa gtctttcgca ggatgcttct gattgttctc tccgcagttg
     3781 cagtagtgtt ttttctgtgc ctactaacca tgtggcgttt ccggcccaac gttgtgaact
     3841 tcctagaaaa agcatttcac ctgtccaacg atgaagctgg tcgcatttgg caagaagtat
     3901 tcagcgacaa tgcccttcta tacgtgttca tcgctatcat cgttttcata ctgattttcc
     3961 tcaggttctc gctgtcatgg tttgccaaat acttcgacga gatcagtgcc gggatcgatc
     4021 agcttgttga caaacccggc agcgacataa tcctatcacc tgaactagca cacatggaaa
     4081 caaagctgaa caagataaaa ggaacgctgg aaagacaaaa atgggatgcg caggaagcgg
     4141 agcaacgcaa gaatgacctc gtggtctatc tggcacacga tatcaaaacg cctctcacct
     4201 ccgtcattgg ctacctgagc cttctggatg aagcccccga catgccgccg gagcaaagag
     4261 ccaaatacgt gggcatcacc ttggaaaagg cctaccggct ggagcagctg atcgatgagt
     4321 tcttcgagat cactaggttc aacctgcaat ccatcgtact gaacaagggc aagataaacc
     4381 tctccttcat gttgaaccaa gtagcggatg aattctatcc catgctggag cccgagggca
     4441 agaaagcggt cgttgatgca cctgatggcc tcacattgtg gggagatgca gacaagttgg
     4501 cccgcgtttt caacaacatc ctcaagaacg ccgccgccta cagctatcga ggcagcatca
     4561 tccgcatctc cgcgcgccag gaagatcaaa gcgtaatcat cgtctttgcc aatcaaggcg
     4621 atcccattcc atccgccaag ctagagacga tcttcgacca gttcttccgg cttgatgcct
     4681 ctcgttctac gaattctggc ggtgcaggat tgggccttgc catcgccaag aagatcgtta
     4741 ctgcacatgg aggcaacatc acagccacaa gcaacgagga acgaacggtt ttcaccgtta
     4801 cgctcccgaa tcaagatgga acagttggat aatcatgatg caattgcccc cgctctgcac
     4861 catgcactcg cgacgcgaat cacgtcgccg caattcgcca tgtcatccga attgcgcgag
     4921 acatgcggag ccatacccgt gattggctat cggctcaccc atctcgttcc tcatatctct
     4981 cctcccatgt tgctttcctg tcttgtgttg gcttccccca cacactttta tcccataaca
     5041 atgcgccaat cgggtgaaac caccttgagc agaatcaaat gcaccggccg ccgagattaa
     5101 cagaacaatg aatcgtccgt aagtatttct taagatttct acaagcagag cctacttttt
     5161 agcctgcctg attcggataa ggttagatta acgaatcacg caggcgtnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnntag attacgttgc gtgtttgaga aggaaacagc
     5341 ttgcggaggc cttcatcccc cgccgctgtc aacccgcaga acaccacgta aacgccaaga
     5401 ccgtagagac aaggagtcat gaacatgaaa cgaacaagag tagccgtgat attcggcgga
     5461 tcttccacgg agtatgaaat atcgctgcag tcggcctatt cggtaatcac caacctcaac
     5521 cctgaagaat acgacttgat actgctcggg attacccgcc agggagcttg gatgagattc
     5581 accggtcctg tggagcagat caaggacaat acgtggatgg atcacagcgt ctccgttccg
     5641 gccatcattt ctcccgatcg aaatacccat gggatacttg aatttcacgg caacaaggta
     5701 accacgaccc gaatcgacgt tgcctttccg gttctacatg ggaagaacgg cgaggacgga
     5761 acagttcaag gcctactgga aatggcgggc atcccctgtg ttggatgtgg cgtattgagc
     5821 tcagccatgt gcatggacaa ggacatagct cacagaatag tcagcctggc tggagtaaaa
     5881 acacctccat cgattgtgct gcactcttac gtctccgaag cggagctgct taagcggatt
     5941 gcgcacctga aatacccact ctttgtgaaa ccggcaaatg ccggctcctc cttcggcatt
     6001 actaaggttt cccaggaaag cgggcttgcc tgtgcagtca gtgctgcctt cgaacacgat
     6061 agaaaagtag taatagagga atcaattgat ggattcgagg tcggctgtgc tgtcctcggc
     6121 aatgacgtac tcaccattgg agaggtggac gagatcgagc tctgcaacgg cttcttcgat
     6181 tacaccgaga aatatacgtt gaaaacttcg aagatccata tgcctgcgcg gatcgatgtg
     6241 gagactgctg cacgcatcaa gcaaacagct atcgtcatct acagggctct ggagtgttcg
     6301 gggtacgcgc gagttgacat gttcctcact cctgaagaag atatcgtctt caatgaagtc
     6361 aacaccatac ccggtttcac cacccatagc cggtatccca acatgctaaa ggggattgga
     6421 atgagctttg agcagattgt agacacgctt ataaggctgg cgatcgaccg atgagaacgc
     6481 tgaaactcta tgcagaggac attggcaagg gcaacctcat cctcgtcaat ccctctcatc
     6541 cacttgcgac gaaaccagcc gatgaagtgc ttgtgcctat tcgccctgcc tttcccgaga
     6601 tcgttctaga aaggcaaaca gcaaggatgc tggcagaagt ggctgctctc ttgcaatgcg
     6661 aggatcaaat cgtaccagtc agcggttatc gcacgatgca ggaacagcag gctatatacg
     6721 ccgattccat gcgcgaaaac gggaaggagt ttacgaagaa atacgtcgca actgtgggat
     6781 gcagcgagca ccagaccgga ctagcaatcg atttgggaga aaacaggccg gacatcgatt
     6841 tcatccggcc atacttccct tacaccggaa tctgtcaaga gttccggaag ctgtcagtac
     6901 agtatggatt catccagcgc tacccggctg gacgggaatc catcacccaa attgcacacg
     6961 agccctggca ttttcgctac gttggctatc cccattccca actgatcagg ggaaatgagt
     7021 ttacgctgga ggagtatact gactgcctca agcactttcc cttcgacgga gcacatcttc
     7081 ggatcaagca cgacaactac gacttcgaga tttttcatgt ccccgtcgaa ccaggtcagg
     7141 aggttgaaat cgaactgccg gacagaatcc cttttcaggt atccgggaac aacgatgatg
     7201 gctttgtggt gacattatgg aggcatttgg gatgaatctg caggaagagt tccccggtat
     7261 tgattacttc agaataatcg ctgcatttct cgtagtggct aaccacactt cccccctgtc
     7321 gtcagtgaac gggaccgctg atttcatcct gacaaggata attgcccgtg tagcagtgcc
     7381 gttcttcttc atgacctcag gatttttcct gtttcgtgga aaggcgacag acagcgaagc
     7441 ggaccgcgca aggctgtttc gctttctgag gaaaacggcc gcgttgtacg ccatttcaac
     7501 tttgctgtat ctccctgtca acatctatgc gggaaccttc agaactgaac atctggccaa
     7561 agaactgctg aaggacattg tgtttgacgg cacgttttat cacttatggt atctgccggc
     7621 agcgatgatc ggcgcgacca ttgcctggct gttgctgaaa aggctcggcc ctggccacgc
     7681 ctttggcata gccttcatcc tctacctggt cggcctgttc ggcgacagct actacggaat
     7741 tgcagaaaga gtgccattcc tgcaatcgtt ctatgcatca gtcttcacag tttcggacta
     7801 cacgcggaac ggacttttct tcgctccggt ctttctattg cttggcgcca tgatggcccc
     7861 gccagcgagg cgcctgcagc caaaggcgtg ctttacgggg ctggcagttg cgtttcttct
     7921 tatgctggca gaagcgcttc tccttcacac ctgcgggatc ccaaggcacg atagcatgta
     7981 ccttacgctc ctgccctgcg cggctctcct ttttcagagc ctgctacaca tcaatggcaa
     8041 aacgcgcaaa gagctgcggg acatctctac gctcatctat ttagtccatc ccatgatgat
     8101 catactcgtt cgatggcttg ccaaagccgc gggcctcaaa cgatggctga tagctaacag
     8161 cgtcatccat tttcttgccg tagcagtatc ttcccttgct gccgcagaag cgatcctgtt
     8221 gttgctgcga gtctttcaaa acagagagct cgctcctgaa cacgccacta tggacagggc
     8281 gtgggctgag atcgacctga cctgcctgag ccacaacgtc caagtcctgc gcaaagctat
     8341 gccggatgga tgtaaactaa tggctgccgt caaaggcaat gcatacgggc atggcgatgt
     8401 ggaagttgca aaacatctga atcagatagg aatcgatgcc ttcgcggttg ccgctatcga
     8461 tgaaggaatt cgcctgcgaa aacatggaat cagaggaaca atcctgatac tcggctacac
     8521 tgtgcccgaa agagctggcg agctcgcccg ctaccgtttg tcgcagacag tcgtcgatcg
     8581 agaacacgct gtgcgcctca atgcttgcgg gaaaccaata cacgtccaca tcaaagtgga
     8641 caccggcatg caccgtatgg gagagccctg ggatcatgta tcggagattg ccagcatctt
     8701 cggcctgaag aatctttatg ttgacggaat ctacactcac ctttccgtct cagatagcat
     8761 ggaaccggaa gacgctcaat ttacagaaat gcagatagat aggttttacc atctcctgga
     8821 gacgctccgg gagcaaaacg tcacccttcc caagatacac gttcaaagca gttatggcct
     8881 ccttaactat ccaatgctta aatgcgatta tgcaagagtc gggattgcat tgtacggcac
     8941 actgagctcg ccccatgatc agaccaggct ccacctggac ataaaaaatg tcctgtcgtt
     9001 gaaggcacgg gtttcgcttg ttagaaccgt tacagccggg gaaagcgtcg gctatggtcg
     9061 ccacttcatt gccgagcgtg atacttgtgt cgccatcctc ccaatcgggt acggggacgg
     9121 gattccacgc aatctctcgg gcggcagagg ctcagtattg atccatggat gccgtgcacc
     9181 cattataggc cgaatatgca tggatcagct gatggtcgac gtcaccggga tcccggacgt
     9241 gaagcgcggc gatgtcgcca cattgatcgg cagagacggt tctgcggaga tcacggcaga
     9301 acaagcagct gccgatgccg gaacgataac caatgagttg ctcagccgga taggcatccg
     9361 cgtgaagagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccaccgggcg
     9481 tgaattattc aagtgtggcc tgccgccgcg tgagtcggcg gaagacctta agagaccttg
     9541 gtaaaaggct gtatgaatga gtacgatggg gcttgagatc gtcaattctg agcatttcgc
     9601 gggagcatac cccatagacc attccggact gccgataact ggcactcacc gatcattcgc
     9661 accaggctac aacgcgcccg agtgatgatg gcatctgacc tcatgcccct tttccacgat
     9721 gctcacatcc ggagccctct caaaacacac atcctctgaa aagtcacacc ttggagcgaa
     9781 actgcacccc gggcccaggt ttgcaagatc cgggacttcg cccattatgg gcctgagagg
     9841 cttcctctct cccagcacgg gcatggatct gagcaggccg cgggtatatg gatgagctga
     9901 tccctccata accgcttctg ttggccctcg ctccacaatc tgccccgcat acatcacagc
     9961 tatagaatcg cagaaatctg ctgccacagc caagtcgtgc gtgacaagga cgacagcagt
    10021 tccggtggtc tcgttgatct catctatcaa gtcgagcacc tgagcttgga tggttacgtc
    10081 cagtgcagta gttggttcgt ctgcaagaag aagcttcggc ttgcaagcca aggcgatcgc
    10141 gatcattatc ctctgctgca tgcctccgct gaactggtga ggataatcca gatgtctctg
    10201 ctcgggcatg ggtattccta cactctgcat cagctcgaat acatactcac gttcttcggc
    10261 ggcagacagc ttacgcgatc gtgtcttcct cattctcggg cccaacccgc ccccatctcc
    10321 gcataacctt ccggtgtcac ctgcagatac tggaaccata ccatgcaccc gcaaagattc
    10381 tgctatctgt tcaccaactc gataggcagg attgagcgcg gtcatcggat cctgaaatat
    10441 tatggcgatc tccttgccac gaatcgttcg catttctgcc tcagaaaggt ctacaaggtc
    10501 agtccctcca aacataaccc tgcctcccac aatgcgtcca ggcgacggaa gaagccccat
    10561 gatggatagg agcagggcgc tctttccgca ccccgactcc cccactactc ccactatttc
    10621 gccttgagag acagaaaagc ttacgttcct cacggcctga acctccccgc gcgcagtcgg
    10681 gtagattgtc tgtaatccag agatttcaag cagcgtgcta tctgcggcgc acgggtatgc
    10741 cattttccat catccttcca tcatcttcgg atcaagcacg tcgcgaaggc cttctcctaa
    10801 caagttcatg cccagcacca agagcgcaag ggctattcct ggcagagttg caacccacca
    10861 agcgcccagc atgtacctgc gaccatctgc gatcatagat ccccacgcgg gtattcgcgg
    10921 aggaatcccc aggcctagaa agctcaaaga tgattccgtc atgatcatgt tgcccatgcg
    10981 gagcgtccca agcaccagca ttgaattgga gatgttcgga agaatctccc ggacgagaat
    11041 gactccatcc gagcgtccaa gagctcttgc tgcatcgacg tattccctat tcttctctga
    11101 catggtaagc gcccttgcca accggtaaaa ctccacccag cctttgaagc tgagagccca
    11161 cacgagattc caaaaaccag ggcctattac cgacatgatc gctatgacaa aaatcaggta
    11221 tggaaatgca aggagcaact cagtgaagcg cgcgagaacg cggtccaacg ctccaccgta
    11281 ataaccggcc aacattccga ggccgatgcc gaccacggac gatatcaaga tggtcgtaag
    11341 ccccacgagg atggaaactc ttgatccgta gataatccga gacagaatat cccgtcccaa
    11401 ctggtcagtg ccgaggaggt gacctgatcc gaaccccggc gggatcagcc acgagtcgag
    11461 gtcaacctca tctggagcgt acggagcgat aacaggggcg aggaccgcca caagaacaaa
    11521 cgcggcgacg attatcagcc ctgttctgcc accaccgtga gccaacaggc gcttcaccgt
    11581 tcccctgcgt cgcgacgcgc gcacatacgg gacggtacgg gctgtcgata ttacgccttt
    11641 gcacagttca tctgatggaa tagaaacgtg gcccattttc cccatctcct acaaacttat
    11701 cttcgggtcc aaaacagtgt gaataatgtc ggcaacaagg tttataagga cgaacgtgag
    11761 agcataaacc atcaccgacg cctgcactac cggataatcg cgggcaaaga tagcatccac
    11821 gactaccctg cccaagcctg gccatccgaa gatcgtctct acaatcatgt ttccacccag
    11881 cagagagccg aactgcaaac caaccagggt cacagttggt atcattgcat tgcggagcgc
    11941 atggcggcac gtcacgtccc tctccggaac acctttggcc cttgcgaact gcacatagtc
    12001 ggcactgagc acgtcaagca tgcttgagcg cgttacccga gccactactg ctgcaactcc
    12061 tagccccaac gtgatcatcg ggagacaaat gtgtttcaag gcattgccca gagccgggag
    12121 gtttccggtg atcaacgcat ccagcaccag caatccggta acgtgcgctg gctgtgctcc
    12181 atatgctatg cgcccggatg ttggtagcca gcccaatatc attgagaata taagaatacc
    12241 gactattcct aaccaaaagg cgggaagcga tacgcccacg agcgacaagc aggttccgac
    12301 cctatccatg aaggaattcg gatttagggc agacgcaact ccaatcggaa tacctactgc
    12361 caatgcgaac agcatggcaa atacagccag ttctatggtg gccggcagag cttcccggat
    12421 aatgtcggcc acgggacgag acctgacaat cgaatacccc aggtcccact tgaaaacctg
    12481 cttcaagtag gtccaaagct gcacgtgcat gggctcatcc agtcccattt cccctcgaag
    12541 agccgccatt tcctcttcag aagtccacgc tgcctccccc atcatgatgt ctactgggtc
    12601 accagggatg gccctcatga agatgaaagc ggctactgcc acaccgagcg ccagcggtat
    12661 ggtggccagg attctctcta ctatcttggc ctgtgtgcga ctgaggcgga tcatttggtg
    12721 gagtaacctg cctctgcaag aagcgccttg gcgcgcgcag gatcatactt gtacggaaca
    12781 atgctctggt tgaaaccaaa cccgctcggc aagaaggcgg ttgcaagacg cgtggcgttt
    12841 ccggcataga ctgtcctcaa aattgcgtcc cagtccactg cgtaattcat ggccctgcga
    12901 acccttggat catcaaaagg cggacgccta caattcatct ctaccatgta aacacgggtt
    12961 ccctcaaccg atttgacctg gatgttcttg tcgcggacca aggctgctgc catgtgcgac
    13021 ggaaccgact ggatgatgtg gacctcccct gtcttcacag cagccacacg cacagtgggg
    13081 tcaggcatca tcctgaagat cgcccgctta acccgagccg gccccaccgg aggaatagag
    13141 ggagatccgc cgtagtagtc gtcgaacctc tccattacga tcttgtcatc aaattgcccc
    13201 tctacatact tgaacggtcc acagccaaca ggctttcttg caaacgcttc atcgccaact
    13261 cgctccacgt attttcgcgg cactatctga aagtggacca gtgcctgcgg caatacggga
    13321 aatggcgatg acagaacaaa cctgaccgta tactggtcta ccttctccac tttttcgacc
    13381 ggacccaaca ggcctttgcg cggcgatgtg cggccaccca tggcgccttc cctcagcact
    13441 cggtcaaacg tgaaaaccac gtcatcggca tcgagcaagt ccccattatg gaattttatg
    13501 cccctgcgaa tcctgaatag atactctgta tccgagaccg ctttccacgc ttctgcaatc
    13561 tccgggacta ccttcccatc ccatgtgcgc gtcacgaggg cgtcgaacat attgcgaata
    13621 accgtttcag tgggtctgtc gcgataggca gcgggatcca atgtcagtat cttgtcggcg
    13681 tacaggccca ctacgatcgt atcgccccgg gccaacccaa catcgtgcag gttgacgcga
    13741 gaatccattg acggctgcca gttgagcacg tccgaccgtg cggcttcgac ttccatcaga
    13801 gagtatccga atatggccgg agcatcatcg tacaggatct gctgggcatc gaaatacgcc
    13861 tccctgcgcg cgtcgttatc gacagctcga agagctcgat cgagggccgc gtcgaaacga
    13921 gcattgctat aaccggagta gttgccccgg tcgccactgt agaatttggg cactatgatg
    13981 tcgaaaggat cgaaataggc gcttccccag gcagtcaagt acatctgtcg ttcacccgcc
    14041 tgaatccttt ccttgagcgc attccactcc catatccgca catcggcaga taccccaatg
    14101 gcccgcagat tgtgagctat cgcctgtgct tcatcggcgc gggtcgagtc cgtatctatt
    14161 actacagaca gttgtttcct ggtttgaccc gtggttgtag gcagaaagcc aaaagccaga
    14221 atcatggcta taatgagcgt tgtcagaata gtccgtaatt tcatgacacc aacctcctaa
    14281 gacaagcttc cttgggaaaa accgattcat catgcttcat gacgtatgca attctcttgg
    14341 ggaaaaacgg gggcagtctg cggacgcctg ctaagcgcgg acttaaaact tgagcccgca
    14401 cagtatggcc gacgccatac aggcgacatg acaaaaaacc cagggacatc cctgggttaa
    14461 atgggcgtcc cttcttagcg cttacgaggt tagctgacgg gctcgggctg aaggaattag
    14521 ccctacctgc acacctgcag gattcgcccc tgccatatga tatgggtccc ccgctctccc
    14581 ctgctgggaa gattcagcga ttgcgcctgg cggcgcatga tcataccctc tatgatctac
    14641 tcttctttga tctatgctac caccaaaccg tcacgttgtc aaattgctcc tcagctgggc
    14701 tcaaactaca tattcgctgc tctcgcggat caactttgta tgaattatct taaaagactt
    14761 ttgtgcattg gcttctttca tcgcgtaggg atgttataat gctttcacgg aggtgggttt
    14821 gatgactgcg cagacctgtc gcaagcccct ggtttcagcg gtaggtgtat ccaagcattt
    14881 tgtaatgtct gaatcactcc tttcacgcag gctgacaggc ctctccgcca gacaagtaat
    14941 ggccgtggac gccgtgagca tggagatata ccctggtgaa acagtaggtc tcgttggcga
    15001 gtcaggctgt ggcaagagta cttttggccg cgtcatggtg gggcttctcc agccaacatc
    15061 agggcaagtg tattatggca atacccctat ctggggacag aagtcgatcg atcgttcaga
    15121 attgaaacat cgcgcccaga tcatattcca aaacccttac agctctctca acccgagata
    15181 tactgtgcgt caaacaatcg atgtcgcgct tgctgccaga ggcgttcctc cccgccaccg
    15241 ctacgaggaa gcacgttcct tgatgacccg ggtagggctt tcacctgact accttgagcg
    15301 ctatcctcat caactcagcg gcggccaacg ccagcgcgtt gcaatagcga gggcgcttgc
    15361 cactcagccc gagttcatag tcgctgacga acccctatca tcccttgatg tgtcggtgca
    15421 ggctcagata atctcgcttc ttgaagactt gcagaacgag tacgggttat catacctcct
    15481 catcgcccac gatctgcgcg tggtctatca catgtgcaac agggtagcgg taatgtacgg
    15541 aggccaaatc gttgagtttg ccccaaccga cgctctatac gaaaggccgc tccatccgtt
    15601 tacacgtcag ctacttgggg caataccggg aactgggggg cgtcgccgcc ggaaccgaaa
    15661 cggccggggg cacagtccgg cgcaggcgtc tttcgctaca tctattgaag ccgagagcat
    15721 gatcgatagc agccaggcac acattgcaaa cgcgtgtcaa ttctggccaa gatgcgaaca
    15781 ggccgccccg gtctgcaaaa aatcgtcacc agaattgctg tcgctatctt tcggctccgc
    15841 tagcgccttt ggcgcatctc actcagatac ggcctctgga gctagcaccg aacctcaaga
    15901 tgatcacttg gtcaggtgcc acatggcaca taatagacaa tgatttaagg ttgtggcgat
    15961 aaatgaagct tttcagtgcg aacaagagct ttctaggcag gtatgatcgc atgttagctg
    16021 cgtacgcggt cacactgctt gttatgatgt gcggacttgt tgggttctcg gccgccacag
    16081 gcgccgccgg aaatggagtt gacgaggcac agatggttct cgacaacatg ttgcgcgctc
    16141 aagagaacct gaccagcttc gagctccctg caactgcccg agtgtatctg ggcggacgaa
    16201 atgtcctgac cgccaatgta caagtgttag cctcccgccc caacaacctt gcagtcaaat
    16261 ggctcggcgt gaccattcgt cccaggaagg gcataatatt catcgaccca gctcaattcg
    16321 tgtctgagaa ttacacgctt tctgtcttag cccgaccgag caccgcaaga cagccatcgg
    16381 ttacgccgcg ctttgaagat gggcatagca acgatgggtg ggtgatatcg gccatctccc
    16441 gtcccggaac cgcctccccc cttcgctgga agctctatgt cgatccggaa acatggctga
    16501 tcgtgcaagc tgaggtgatc actgcggatt ctgacacctg catcatcaaa gcgaagtaca
    16561 gaaacgccgg ataccgcaga tgggaaccgc tcttcgttca tgctgaaggg aagatggttc
    16621 tggaggagtt ccttccggac gtccttgtag gcttgatcat gggacaggcg gcccgggaga
    16681 agtcggccta tgttgacctc gatttctctg atggacgatc gaaaaaagcg tagctgtcat
    16741 tgcaagctac gcttgaaaca cgatcacagc aggtcgcaca caccccacag agcccgagtc
    16801 agtcaccgac cgggccccgc cctttctttc tggcgacatc ggggcattgc ttagagaacc
    16861 gactctttca actcttttcc cgctctaaac accgggacat tcctggccgg aattcgtatc
    16921 tcctccatgg tcttgggatt ccttcctacc cttgcctctc tcttcttcac ctcgaagctg
    16981 ccgaagccga cgagttgcac cttctctccc gccttcagct gctcctttat ggtttcaagg
    17041 atcgcgtcta ctgttcttgc agtgtccttc tttgtcattc ctgtcttgac ggcaaccgaa
    17101 tccacaagat ctgacttggt catgtcctca gcccccttcc atcagtatgt tttcccgcga
    17161 cgtaagcctt tattacttcc gcctcaaggg ctttcgaaaa tggccatatc aaacaggacc
    17221 gggcgatgcg gtcagatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt
    17341 actatgcaga tcagacctcc gctgccttcg ttgacgatct tctgtaatgt ctcttgaagt
    17401 ttctcctgag cgtgaatggg catgtgtgcc agtttggcct cgatgccttc gcgtatcaag
    17461 tcttgcatcg accttccgag gaactcggtc tcccatatct tcgacgggtt ctccgcgaaa
    17521 tcttcggcta tctgctccgc aaattcctcg ctttgttttt cagtccccat gagcggggtg
    17581 acttccgtcc ttatgtcggc ccttatcata tggatcgagg gcgccatcgc cttaagcctg
    17641 accccacacc gattaccctg cctgattatg tcgggctctt caaagtagat ctcgtccaga
    17701 aatggcgcaa ctataccgta tcctgtcgtt ctgacctgtt caagcgccac agcaacctta
    17761 tcgtattctc gctttgcatg tgtcaagtcg cgcatcatcc tgatgacgtc ggaatctccg
    17821 gccacctcca gtccgctcat ctcggatatc acagcgtaga aaagcgactt gggggcagca
    17881 acttcgatcc ataccctacc ggtgcccatg tcaagaaggg tcaccgtcgc tgactcggca
    17941 ttcttagccg ttccgagaat ccgcactgcc tcagaaacat ctcgcacttt atcaattgcg
    18001 ctgaccgcct catgtactat ggagacgaat cccgtgcgaa cagggtgcga atcctccagc
    18061 tccaccaccc attcaggcat ctttacactg atctcgcgaa tcggaaactc atacagcacg
    18121 ccgccgagca cctgcatgat gtcggccgta gacatgttga gacaatcaac gggtatgacc
    18181 ggcacgtcgt aacttctcgc caaagcctct ccaagggtac gtgtggattc cgcttcagga
    18241 tgcgtagagt tcaagacaac tataaacggc tttcccagct ccttcagttc cgctataact
    18301 cttctctcag catcgatgta tgcttccctt ccgatctcag taatcgaacc gtctgttatg
    18361 accaccaacc cgatagtaga gtgttcctga atcaccttct ccgtgccaat ttcagctgct
    18421 ctttgaaaag ggatctcctc ttcgtaccag ggggtccgta ccattctggg agcgtcagcc
    18481 tcttcatacc ccagcgcccc aggaaccgtg taacctacgc agtccaccat tctgacagcc
    18541 actttgatgt tctcccccag cgtgatctct accgcctcgc ccgggacgaa cttgggctca
    18601 gtcgtcatga tggtctttcc ggcgccgctc tggggcagct catcgcgggt ccgctccttt
    18661 tcgtaggcgt ccttcatgtt cggcagaacg atgaggtcca tgaacctcga tatgaaggta
    18721 gatttgcccg tcctcaccgg accgacgaca cctatgtaca cgtctccccc cgtgcgttcc
    18781 gcaatgtgcc ggaagatatc gaacttctcc atccattcgc cctccctcgc atcgagtccg
    18841 ggtggaggcg gcctcacacc cctgcaggat gcacgtgggt tgtacctgct ctcagacgct
    18901 acatctatat gagacttggg gggaattatg ccggattcag atgagcaaag gaaggagatc
    18961 aaccatggca acaagatcga gttcattgtg gtcgagcacg gggatgatct tctccgcatc
    19021 tcccgtcctg acccagtcat tgtagagccc gggaatgagc gaaccgggga tgtctccgtc
    19081 ccttgggaca tcgaggacca tggtctgtat tgtggagagt cgacaattgg gcaaggaaaa
    19141 ggagtatttg cgtctggccg ggggacaaag atcaacgatg gcagcatgcg ggttgtcttc
    19201 ccgtccatag taagccaggc gcctcagaag aaacggcata tcgaagctct tgccattata
    19261 ggttacaacc gcccgcgcgt cggcgagccg cgcgagcgtc tcctcgataa ctgcaggctc
    19321 ctcttcatat tctctggcga agagttggtg caggatgagg cctcgttccg acggaatgat
    19381 catgcccacc atgaacagtg ccgaagtact ggaaagcccg gttgtttcga tgtcgaggaa
    19441 aacaacttct tcgggggcgc aaaaggccaa aatatccagg ccaggcaccc cgtagcgcaa
    19501 aagctcccat ccatcagcgc gctcgagagc ctccacgata ggacgggctc cctggcccca
    19561 tttttcatgg gaaagcagat cgaaaagggt cgcatagccc tcttccttaa gcccagcctc
    19621 cctcaccgga ccgatcccgt aaaccagctg cagattccgg gcaatgcggg agacagcctc
    19681 ctcgggctcg ggaatgaccc ccacgaactg gctagccatg tctttctcta ttgcaatgca
    19741 tgtgcctgac gaattactta ccacggcgcc acggggaaaa gagtgcaagg ccgaaccact
    19801 cctcaaagtg tgtatctaac cctgaaaaga atatttgcct gcccgcctgc gggaacattg
    19861 acactgaact gcgcccgccc cgagacgatc ggaggagttg gaatattgct ttgaagaata
    19921 gtccactgcc ccggaaactc ctccacgttg atgacggtga cactcgctgt tccggcgttc
    19981 gctatcctca cctggtaggc ctcctcccaa ctgctcgtgc ccaccttgcg ctgatctgtg
    20041 cgagtccgtt ccgcagtaag caaaacagca ggccccacct tcgcgatgag tgttgttccg
    20101 ctctggcacg gggaaacaag gtcttcgccg aggaaaaatg tcgtcccatc cgaagatagg
    20161 ctgaagacgg atatgcgtcc cccgggaaga gggtacgcca acccggtctc cttggacgcc
    20221 gaaaactcca agccgagcat gacggggcgc tcgccctttc cttgtgacga agaatcgagt
    20281 atcgccatcg gtccccaatc aactacatag acgaggcgcg cgggcactgc cgctccgccg
    20341 gcgaacctga tattccgctg cgaacccgct ggaatcatct caggagaatc tattgtcgca
    20401 accgtcttcc ccagggcaga atcccccttg aagcccggct ttgacccgag aggattagtg
    20461 gatccaccca tcaagactat cctagccggt gcgagatcac agtctgtctc gttatgtatc
    20521 gaataccacc cccagatccc agcagcgtcc ttagtaggct ccagcaccgc gttgtaggat
    20581 gcgctccatg tgagccctga cagcgggtag gccaccctga agactgcatg ggacgcggtt
    20641 tcagccttca cactgaagat gagctccccg gcagccctgt ttgttgccgc agatactaca
    20701 gtaaatggga cggtacccga aatgatctcg gcagcgccgc actgtattgt gtcggaaacg
    20761 ccagcaactc ttaccttatt catgcccgca acgagatcca cttcaacgac atgcgtagcc
    20821 actgcaagac cagattcttg tattacaagg caatcgaggg ttgcaccatc attattcccc
    20881 agagcccgat cggcacttgg gagctcaagt aggctgcgta ccgtctcggc tgcccaaacg
    20941 gcatctgcgc catgcggcgc tgccagtgaa accgccataa tgcaaagaaa ggcgatagcg
    21001 caaaggctcg tgcgcaactt tttcgcaggc aacatgggac aatcaacctc caatactcaa
    21061 ttggcaacac gtgcacaggc tattatcgtc tactccctac ttctagcttt ctccttcaca
    21121 tcctgcgcga acttgagcat ggcaaattcg acctgatgcc tgatgagtcc gccctgcaag
    21181 tatgcgtcgt aaggcggccg aagcggcgca tctgcggaaa gttcaattga cgatccctgg
    21241 acaaaagcgc ctgcggccat tatgacttca tctgcgtatc ctggcatcgg cgctgctatg
    21301 ggacgaacag ctgcatcaac cggagacgcc gattggactg cttggcagaa tgaggagacc
    21361 acgtctgccg atccaagcct tacggcaagt actatgtcgt gacgtgcctg gcccggacag
    21421 ggtgaaacct catatcccag cttacttagg aaggccgccg cgaagatgga gccggcgacc
    21481 gcttcgccca caatcagtgg agacatgaag agaccctgag ccagctgacg cgtatatcca
    21541 aggctcgggc cgcattcaca gcctagtccg ggggctgtta gcctttccgc cgccatctcc
    21601 actatatcgg cacgtcctgc aatatatcct ccgcacggag caatgcctcc gccagggttc
    21661 tttatgagcg aaccagccgc cacatcagcg ccgacagcag tgggctcccg agtttccacg
    21721 aactcgccgt agcaattgtc gacaaagact atagccttag ggttagcctc cttgacgcat
    21781 ctagcgattc tgccaatctc atcaatgccg agggactttc gccacgagta tcctggagac
    21841 ctttgcacca tcaccatcct ggcgcccctt gaagcgctcg caatttgctc gaagtcaggc
    21901 ccaccatcgg cggcaggttg cacctcaagg taatctatgc cgtattctgc caaagatccg
    21961 cgcaccggcc tgtcccgtcc gatcatggtg accattgtgt cataaggcgt tccaaaggcc
    22021 gacaccagaa gatccccggg gcggagtaca ccgaagatga ctgctgccaa cgcatgggtt
    22081 cccgaaacaa tctgtattcg caccaatgcc gactcggcgc cgaatacgtg tgcgaatgcc
    22141 tcttcaaggc agtcacgccc gatgtcgtca tagccatacc ctgtcgtgcc cccaaaatga
    22201 tagtcgctca ggcctgcctt ggctaaggct tcaagcaccc gagcggtatt aattctgcat
    22261 atcgaattta ctgtcgctat ctcgtccgat atttcttcgt gtacgcagga agccaattcc
    22321 atgatatcgt catcgacccc gagaatttgg gccacttcac ttcgatccaa cagaaaatcc
    22381 cactcctttg agccagctct caatagacgc tgcgatttca tctgcaatat cttcaggccc
    22441 tctgccacac tccacatcga tccaacgtat gttgggattc ctgcggaacc atgtcatctg
    22501 cctcttcgca tatttcctcg tttcccgctt caatgtgtcg atcgcctctt cgaggctctc
    22561 ctccccggca aggtacaagg caaactcctt gtatcctata gcctgcactg cagtgtatct
    22621 cggatcatag ccgtgttcca tcagcgagcg gacctcgtcg atcaggccct gctcgaccat
    22681 aatgtccaca cggtcgtcta tccgcttgtt tagcgcctgg cgcgccatgc gcagtccgta
    22741 gatgaccgtg tcaaactcgc tctctccttc gtgtttgcgt tggagctcgg atatggccat
    22801 cccggtcact tcgtaaactt ccaacgccct cactattcgc ctcaggtcat tggggtgtat
    22861 tcgctctgcc gcaacagggt ccacctcggc aagacgaacg tgcaacgcag ggctccctgt
    22921 cacttcagcc tctccctcaa gacggcggcg tatgtcggga tttcgccctt catcggggaa
    22981 taggaaaccg cggacaaccg cgtccacata taacccggtc ccacccacca tgattggcag
    23041 tctccctcga gccgctatgt cgcgtatggc ttccctggcc atctcctgat accgggctac
    23101 acagaagatc tcgtcggggt cagcgacatc tataaggtga tgggggatct ccgcccgctc
    23161 cgcttgggaa ggcttagctg tgccaatatc cataccccgg tagacctgca tggaatcggc
    23221 tgacactatc tcccccccaa gcttgcgcgc aagctccatg ccaactgcgg acttgccgac
    23281 agcggtgggg ccgactatga cgagaagcgg cggccttctt tgaaagtcac gttcacccgg
    23341 atgcccagcc ccatttccca ctcggcctat accatccccg acgctctctc cccgaggcgc
    23401 ctcccttacc cggtcagtcc catcgctctc cttcaacgaa tatcaccccg tattcctgcc
    23461 gactcttctt tgccccgatc ttatggccca cgcccagctt gtcaagttca tccccgtccg
    23521 ccctgtcctt gacgacgaca cgtctgcgtg ccaccctgca ggcctccgaa agcgcttcca
    23581 cggaaagtgg cgtgtggcat gcccagggcc gtatgggggc aatacctgcg gatgacgtca
    23641 ctggctccct aaacatggga tcgaaggaga ctacatcaaa gctcttgtct gcacatgacc
    23701 tgagatagtc cagatgatct gaggcgacca cctctatgtg cctcattgca tctaggacgt
    23761 ctggatccgc ctcggtatag ctcgtgagcc cctcacgggt gataatggaa agaaccggac
    23821 tgacctccac tcccacaacc cgtccacggc ttcccacaac ccatgaagag actatagcgt
    23881 ctgacgccaa gccgatcgtg cagtcgagca cgtgatcgcc agaggatatt cccatggcct
    23941 caaccatctg gtctactcca ccttgcttca gtcgacgaat cctcgacaag gccattccgg
    24001 gatggaagaa caactccgca ctgcgcctgc ccgcatccgc caacctcaca acgcttatcc
    24061 tgtccgcctc aacaaccatg aacgcttcca gctcgccacg accatcctcg ctgtaagcgt
    24121 ccgcaagaga ttgcaacgat ctccctgccc tcgttatgaa aggcgcacca agctcgtgtg
    24181 caagtcttat tccaagatca acctgatggc gtccgccccg cgcggatgta gtcacggcaa
    24241 tacggccaac gtccttcatc aaatgtcacg ctacaaagac cccctccctc agatatgggg
    24301 ggaagtagcg ttttccctcc tttcatacag cattggccga tgccgagatc cttgtgccga
    24361 atgtcacaat agcctgtgat acgtcttgcg aaaacaaagc acatatactc aaccttgtca
    24421 aatagctgaa acccatggac acgctaggtg ccctattggc cctccaaaca cccttctcga
    24481 gatctagcat actttaggtg tgacgtaccc ataggcctcg taaacgtgag gtgttctatt
    24541 caccactttt gcctaaccat cagccacagt gcaccaatca gtaatcccct gcctttcagc
    24601 gtggggcggt ttacttgcgc ccaaaactcc ttgccaacgc atcccccggc actagcacga
    24661 ccacgggccg tccatgcgga caccgatagg gatcctcaga ttcgaacaac ctctccacaa
    24721 gagacttcat ttcagcaata gtgagagaat cgcctgcctt caccgctccg cggcatgcaa
    24781 gcgacaccgc gatatgatct gccagcaatc cgggatcaga ccctgaaggc gccgagacaa
    24841 tcccttcgat ggcggccatg agcgactctg ccggcggcac cccggcgctc tgagagggga
    24901 ctcctctgat cacgacagag gtcgcgccga acagatcgag ttcataaccc attctcgaca
    24961 gaacgtctcc gtgtttggcc acgatctcca cgtccgacgc gcctatctct gcgatctcag
    25021 gtgtcagaag gagctggaca gagggcttct ccttcgcgaa ttcctcccgg tatttgtcca
    25081 ccaaaatacg ttcgtgtgcg acatgctgat ctattatgac caagccttcg gcaccttgcg
    25141 caagaatata cgtatcatcc agctgtccaa taacctcagg gaccgcgcct gcaaggcctg
    25201 atcttgccgg agccagccca agagcaacgc ctggtccgga cgcaggcctc acctcacgga
    25261 acgcgctctc ccgatcccat gacgagatgg caagatgttg aggctcacct ccatacgagg
    25321 cgcccggccc ccgccattgc ctagatactg cacccgtctg gccccatcct gctgtgggct
    25381 gaacaggctc caggtctggg acggcatcga ctgaacgcag cacaaaggct aaggctccgc
    25441 agacagcgga gaatacatcg gagttcgacg cgaatctcac ttcactcttg gctgggtgaa
    25501 cgttgacgtc gatctgagac ggatcaatct cgatcgaaag aattaccgga ggtctgatgc
    25561 ctacgggaac agtcttgtcg taggccttgt ctatggcaga ccagactgtc gtggatacga
    25621 ccggacgacc gtttacaaat atgtattgcg agtcgcggcg tttgcccttg gcgtgctgag
    25681 gcaaagagat gaggcctttt acgaagatcc cgtgggagga actctttacc ggaatcatct
    25741 cgcgagctac ctccagcccg aggaccgcgg cagccgcctc ctcaatcgat ccgccttgcg
    25801 gagtgacaag cgccgtgcgc ccgtccacct tgaggctaaa tccgatttca ggatgggcca
    25861 gggccaggct gcccacggtg cctatgatcc tcctgttctc cgtagagacg gaacgcaaat
    25921 acttgagcct tgcaggcgtg ttaaagaaga gatcccgaac ggcaatgctt gtaccacagg
    25981 gtgccccgat agctgtaggc tcccccactc gtcccccttc cgccactacc accgtccctt
    26041 caccgcttcc gcgctcacag gtgacgagcc tcactctggc aactgaagcg atgctcggga
    26101 gagcctcgcc cctgaagccg aggctttcta tggaaaagag gtcttccgcc cgggaaatct
    26161 tgctggtggc atggcgttca aaggcccgcg ccgcatcttc tcgccccatg ccgcaaccgt
    26221 cgtctgtaac catgacaagc tccagaccgc ctcctagcgt ctctatgctt atggtgttag
    26281 ccccagcgtc tatggagttt tctacaagct ccttcacaac cgacgcgggc ctttcaacaa
    26341 cttcgcctgc ggctatctta ttggcgactt caggggcaag cctgattata cgcatccagt
    26401 tcactccttc atcctggcca gatccttcag ctcgtacaca aggtctaagg cttgtttggg
    26461 agtcaagttg tctgcgtcga ccccctccag ccttcccaga actcgttgag ccgtctgtgc
    26521 cagagaaacg tcctctgcaa tcacctcagc agcgctccca cgaactggct cttcgtctac
    26581 ccagccaaac aaggtcatct gatgtccttc cgaaggcgaa gcggcttcca agccttttag
    26641 tatttgctga gctcgcctta ttacctcccc aggcaaaccc gccattttcg caacgtgcaa
    26701 accgtagctc ttatcgacgc ttccacgagc caacctgtaa aggaacgtaa gttcgccccc
    26761 gcgctcaact gcccctacat ggtagttgcg agcatagggc agtctctcct ccaatgcggc
    26821 caattcgtga tagtgcgtgg tgaaaagtgc ccgtgccccc acgtgatcat gtacccattc
    26881 cgcaacagcc caggcgaggg ccatgccgtc gtaagtgctt gtcccgcggc ctagctcgtc
    26941 cacgagaatg aggcttcgat ccgtggcgtt attcagaatg ttcgcgatct ccagtaactc
    27001 aaccatgaaa ctgctcttgc ccgcacttat gtcgtcatac gaccctgtgc ggtaatatat
    27061 ggcgtcgcat atcccgatct cagcatcagc cgccggcacg aaggacccta cttgcgccat
    27121 gacggttatc agcccgatcg tggcaagcac cgtacttttt ccggccatgt tgggacctgt
    27181 tatgatcagc aaccttccac cattgcacga agcctctatg tcgttgggca cgtatctagc
    27241 cgatcccaac atctcttcga gcacggggtg tctcgcgtcg cgtatcaccg tggctttccc
    27301 agtgttgatt tgaggcctta cgtaacctcg gttccgcgca tcctccgcaa atgaagccag
    27361 cacatctagt tccgccactg cctgcgccgt tgcgagtatc ctcgggaggt aagcgctgat
    27421 atcgtccctg acctgctcga atatcgcagc ctcgagcctg ataagcctct cctctgcacc
    27481 gaggatcttc gattccagct ccttcagctc cggcgtaatg taccgctcgg agttggccag
    27541 cgtttgcttg cgagtgtaat catccggcac aaggtggctg tttgcatgag tgacctcgat
    27601 gtagtatcca aacacctggt tgaagccgac ttttagcgac tttatgccag tgcgttcccg
    27661 ctcacgggct tcaagcctta tgagccattc cttcccttcc gtggaggcac gcctgagctc
    27721 atctacctcc tcgctgtagc cttcacagat cactccgccc tcgttagcgc ttgccttcgc
    27781 gtcaagcttg atagatgccc ttatacgttc cgttgcttca ggaaccggat caagcgcccc
    27841 cgctaggtca attatcctgg aagacttcgc ctcaccaaga acagcggcca ggtcatccac
    27901 ggcctcaaga gacgaggcca gcgccaaaag atcccggggc ccagccgtcc tataccctat
    27961 ccgcccgcat gtgcgctcca gatcgtacat gcgatccaat gtcttgcgaa catctgccct
    28021 tagaaacaaa tcacctgcga gctcttccac ggcatccaac cggctctcga tttcagccgc
    28081 cgacacagca gggcgttcga accacgacct gatgagacgc ccgcccatgg gcgtcacagt
    28141 gctatcgagg acggaaagaa gacccctttc gcggtggaat atctccaacg cgcgccgggt
    28201 cggcgtatcc atcctcataa attcgcccag atggtaaacg cgcatccttg aaatctgcgg
    28261 cacgctggtc atctgcgtct cctgcaagta ggatacgagg gcgccggcgg ccctgacagc
    28321 aagatccatt cctgctgcgc cgtaaccttc gagagaggtc accccaaact gggacattag
    28381 cgtctcctca gccctcccac gttcgaaatc cattggctgc ctgggatagg cgacaaaact
    28441 accctcaacc gggcaaccgc cccccttatc atcctcatca aaacgagggc cagacgaacc
    28501 ttgaagccca cgtgaatcac caggatagat acattcggca ggagctatcc tgtcgatttc
    28561 ctctgaaatc aatgcaaacg catcatcgcc gtctgcctgc gtagccgcga gctcaccagt
    28621 ggacgcatcg cacgaagcga gccccacgcc tgactcggtc gcaacaacgg cgatgagaaa
    28681 attgttgcgg ccggggtcca cttggtccgc aagcgtccct ggagtcacga cccgcactat
    28741 gtccctgcgc accaacccct tggcagtgcg cgggtcttcc acctggtccg caatggctac
    28801 cttgtgccca ttctgtacca gcgtggcaat gtactgatca acagcatgat gcggaacccc
    28861 gcacatgggc atcttattgc ccgagccgac ctcgcgtgag gtcaacatta tccccagtat
    28921 tctgctggcg atctccgcat cttccatgaa catctcgtag aaatcgccca gcctgaacat
    28981 gagaatggca tccggacatc tcttcttcaa ctctcggtat tggcggatca tgggagtcgg
    29041 cttagacatc gttgcagtca caccttccat ccgtagccaa acctgaggac ctcgctgttc
    29101 ggccttgcat ctcctctccg gcagcctcgt gcccatcaca ggttagcgta ccccgatgcc
    29161 cactacgaac ctcaatttcc tcgccgtaca gcgtccaagt gcgcgtttgt gaaacgcgca
    29221 gcatgacaat gtcgccagga ttgtgagctt tctcagaagt ccaatggatc atcttgttcg
    29281 ttcttgttct ccctgaaagg accgacgggt cgcgttcgga cggcccttcc accattacct
    29341 cgactatcga tccacacagc tcgtcattga cctcgctagc tatcgagttt acgaggtcta
    29401 tcaggcgata gacgcggtca tgcttcactt catcttcaat ctggtctggc attcgagccg
    29461 caaccgtccc tggcctggga gaatagatga acgtaaatgc cgcatcgaat ctcgcttgcc
    29521 tcaccacatc catcgtagcc tggaaatctt cctccgtctc tcccggaaaa ccaaccatta
    29581 tgtctgtcgt tatggatgcc ccgggaacag cttctcttat ccgatccaaa agcgccagat
    29641 acctctcggc agtatatcct ctgtgcatca tctcaagcac tctatcgctc cctgcctgca
    29701 gaggtaagtg aaaatgttcg cacaccttct ccgatgaggc aacagcctct atcatcttgt
    29761 cagaaaagtc cctagggtgt gatgtggtaa acctgatgcg tctaatgcca tccaccaagt
    29821 tgacttcgtg tagaaggtca gcgaaatcag gcgatgaccc cgacggaagc ggcggcaggc
    29881 cacgaccgta agagttgaca ttctgaccca atagcgtcac ctcgacgcac ccgctccccg
    29941 ctagatcgca gacctcagct actacatctt ccatgcggcg gctacgctct cttcccctca
    30001 catacggcac aatgcagtat gtacagaagt tgtcgcagcc gtacgtgata ttgacgaagg
    30061 ctttgtggcc ataggcccgc acagcaggaa gaccttcaac tacttcatca ggctcgtccc
    30121 acacctccac aacccgctcg ccggcgaaga cgcgctcgag aagctcaggc agcctgtgga
    30181 tattatgagt gccaaacacc aggttaaccc agggcattcg agcgcgcact atctccctga
    30241 gatcgcttct ttgggccatg catccgcaca tgccaaccac gagattaggg ttgccttctt
    30301 tcaagcgctt catctctccc aaattgccaa aagcgcggtt ctcagcattc tccctaacac
    30361 agcaggtgac gaatattaca atgtcggcgt cctcaactct agaagactct tcgaagccga
    30421 ggcttgacag gatgcccata atcgtctcag agtcgcgttc attcatctgg cacccaaaag
    30481 ttaccacctt ggctgatcta cgtctgactt ccataccgcc gcctccataa aaagctatgt
    30541 agatttccta gtctctcaca atcatgtcca gaagagaagc gtagtcaggc acgctttcct
    30601 ttgcagcgcc aggcaattgg acgccctctt ctacagcccg gcgaatcttg tcggatccgg
    30661 ctatgtcgcc aaagaggatg ccgcctacaa ccttgtcacc tgacagcacg agtttgcggt
    30721 acactcggct atcgtaatcg acatctacta tggacgttac gtcgggacca tcctgcacat
    30781 cgccaacgga aaagagcctt gttcccatca ccatcagcgt atatgagggc ggaaccgcca
    30841 catactcttc ttggccaccc gcggcattga cgccagctac cttgccctgc gctaccgcca
    30901 ccggcaggat tccccctaca gagccttcga attcagcaac atcgccggca gcgtatatcc
    30961 acggcgtcga tgtacgcatc aatttgtcga cctccacccc gcgattgatc ttgaggccag
    31021 cggaacgagg aacatcgaga ttcggcctta cgcctgtgga gaacaaaatt acttctccgg
    31081 gaatctcctc gcctcctgca aggcggatcc cgcccggaaa cagagcctcg ctaaaagccg
    31141 atagatgaag gacaattccg gcggattcca cgatctccgc cagcatccgt gatgcatttt
    31201 gatcaagcat cttcgagagc aaacgagagc catgctccaa cagatgaacc tcgagatcgc
    31261 gcttggccag ggcccacgca gcctcaagcc ccaggggacc tgcgcccacg acgcaaactg
    31321 tcttggcggt ctcacaggct gaggagatgg cccgcgcatc atcgacggta cgtaaggaga
    31381 acacattctc cctatcaagc cccgaaagcg ggggcacgaa cggactgctt ccacaagcca
    31441 gtatcacaga ggaagcggaa agctcgctgc cattatccag cgacacggtt ttgcgatcaa
    31501 catcgatcgc cttcgctgtc tgacccagca atacctgggc attgcgctcc tcataccatt
    31561 gcggcttctt aattgccaag ctttcagagg acacttcttc accaagaaga tgtgaaagcc
    31621 gaggacgata ataagcgtag tatggttcct gagacaatat tgtaaccggc acgtccacat
    31681 cctgcgacct tatcccttcc aacgcattga ccgcagcagg ccccaatcct actatgacaa
    31741 ttccaggccc caatgatgca cccatgttgc ctcgccccct ttgtatttca gcattacgaa
    31801 tattatatca tcggcctttc agaagctgaa acgcaaagaa ggcaacatcc cgcttaggtt
    31861 tcgcaccgct gattagccag aacggacgat ctcattacgt cccgcggctt cgactagccc
    31921 cgtgcacgca aaaacgccac caggctcgaa ctagatcgag tctggtggac ctcagtctcg
    31981 ctccgccggt tggtgacgtc cctgaatgag gcaactatta gccgactcca ttgaatccgt
    32041 gaagacttac gtgtcaccaa ggtaaattcg agctctctag gaactgcaac aagatgcctt
    32101 tgaaggaggg cctgccagcg catgccggcg ccctccttcg gatcgtgtgt agaccgccat
    32161 ggcgcaactc atgttttacc ccaacgcgat catcctcttg gaactcactg gcagaggaca
    32221 acagcgtgtt tcactatcca caactacttc ctcatgagac cttgtcgctc agcatggacc
    32281 agactattcg aaaccctgca atgcaagccg tcggcatcat tccatcgcaa tttgctccct
    32341 aaaccaagaa gacgaagatc acgacaagaa ctacggcagc cagtatcaac caccaccacc
    32401 atggaaaccc ccagaagaac cattgccctt tagtttcagg aagcccctcg accaatgaag
    32461 tcacccccga ttcggtttat cgctagaacc accattctat gaaaagaaag atgatcaaca
    32521 tgatcaagag cggaaggagt tttcccaatg cactgcctgg cccaaatata tcgcccagat
    32581 tcacccgaac accccctatc actgctcagg ctggtcctgt ggcggcagga attccggtcc
    32641 aagcgactca aggagttttg gcaacatgtt tgacctcatt ccttcctccg caagcgagcc
    32701 aagaagcgca gcaaaattcg gatcagaaga cgccttagcc atcacgttct gcaacatttt
    32761 ggccacatca tcggcactcg ggccagaggg ccccttaggg ctggttgccc tttccggctc
    32821 cgactcaggc ttttttgaca tctccatcat gctgtccacc gccttcgcaa ctatcttagg
    32881 atcgagttcc atattagcca ggctcgacag gaaggccaaa ggacgatcgt gccccatgcc
    32941 gcgccgtttg gtcaacacat cgctgactga agtgtaaatg cctactaagt taacagaacc
    33001 caggacatca tctaccgccg cctgccccct ctcgctcagg aaaggacgca tagcctttat
    33061 cagccctata ctgtctggat cggtcggaat tctcatgtcg ccacccccat aacgcaaacc
    33121 tcgcgaaccc gttttttcga tactcttgac agggcctgag agacgttgag aactccggca
    33181 ccttgctcgt cagaactgac tccgtctagt ctgtcggcgc ttgcctgcag caatgacttg
    33241 atatctgccg gagacaagct cggatccgcc tcaagaagga gcgccactgc acctgtaacg
    33301 tggggtgccg ccattgatgt ccccgtgctc ttcctgtatt gcttgcccgg ccaggttgag
    33361 agaatgtcaa ctcccggagc cgaaatcgag atctcccgcc ccctgcttga aaagttggcg
    33421 atgttgttat tctcatcaat agccgcaacc gctatggatt ccacgtactt cgccggatag
    33481 cccacactat tgctgccagg cccgcgattg cctgctgcac ataccattgt tattcctgct
    33541 ttgcgcgcac tctgaaccat gtccctaaat gtctggttct cagtgctcga actgaggctc
    33601 atgttgacca ctttgatacc cttttccaca caccaaccga tgccatcgat cagactggac
    33661 agcttcccgg atcctttcgc atccaagacc ttgattgcat agatattcgc tctcggcgca
    33721 accccaagca ccccaatact gttttgcagt gcacctattg tccctgctac atgggtacca
    33781 tggccgttgt cgtctgaggc ggaatcgatt ccggcaatcg cgctatagcc gccctttatg
    33841 ttcccagcga ggtccgggtg gcgcgagtca acgcctgtgt ctaatatccc tacattcaca
    33901 ccatttcccg tttgacttgc gtccccgatg tttatcctct tcacgcccca gggaacgtcc
    33961 tcccgaaacc tcggacccca ctgcactttg cctacctcat aggctaactc tatatcgata
    34021 tcgtcttcca ctctcaccac acctgaacgc gattgagcca acgcctgtac gtctgcaatc
    34081 tcttcttcgt ctaccaaaac caatacccca ttaacgagcg gtagctcacg gagaatatgt
    34141 ccacgacagt tcgcaaggat ctctgcccac tggtttcggc tgatgttctg atcgaagacc
    34201 acgatctttt gctccctagg cctccctgtc gcagagaagc gaaaagactg aacgcccaga
    34261 tttcccccaa gcctctgaca tctacgcatg atcctacctc ccctgcccta cttggacagt
    34321 tcgccgaaag gacgaaacat ccccatgttc ggcagtgtct cgcacttttt ttccaacacg
    34381 gtatcgtctt tctccccggt cagccatagg ctaacggcta tcgttgctat tgtgaggatg
    34441 tcagcaatga attccctgtt taaagactct tccattcaca ctctcctcct tgcgcctttc
    34501 gccgcctgca aacctcaaag cggtatacat tatgactttg gcgtaccttt ggttccatgc
    34561 catgggcgct ttcaaagcca atccggattc tctccggtac ccgccaaagc atttcagatg
    34621 gttgaattgg caaaccgggc actacatgga gattctattc tgtgaaggga gaaatggcga
    34681 tgaaaagacc tttcatc
//
LOCUS       DAOSYL010000019        32656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1122_length_32656_cov_8.210300, whole genome shotgun sequence.
ACCESSION   DAOSYL010000019 DAOSYL010000000
VERSION     DAOSYL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 32656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32656
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_32656_cov_8.210300"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1595
                     /locus_tag="PLU15_04840"
     CDS             303..1595
                     /locus_tag="PLU15_04840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO30280.1"
                     /translation="MDEWFSTDVLPNGINVYVMQTSKFKSNTITAVIYSDLTEDTVTL
                     NAILPEVLSRGTRRWPRTAEFRQNLDSLYGTGLSAGVSKRGEKQLLIFSVDVANDAYL
                     SADEDLLRQGIGILRDVVFDPVLEAEAFREDYVEQEKINLRRRIESIIDNKMGYAFKR
                     CNEEMFRGEPYALYRLGRVEDIPGIDPEGLRSWHKKAVAQLPLDIFLVGDVARERVLE
                     LVGEVFDMERKPMPLEVPARVGHRVPVQPRQVVESMDVNQGSLVIGYWTGVNMSDPDY
                     PAILMANGIYGGYAHSKLFQNVREGESLAYSVYSGIDQNKGVMNVWAGIAPEAMDKAV
                     EIIEEQLDDVRSGRISDDEMDKTRRAITRDIRMMGDSPGAQIDYAIVSRINGVGEKPD
                     ELLRRIEAVSLDDIVRVASKIELDTIYFLRNEEDEAGA"
     gene            1597..2883
                     /locus_tag="PLU15_04845"
     CDS             1597..2883
                     /locus_tag="PLU15_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO30281.1"
                     /translation="MEIARSELLREEVSTTRLDCGLEVCVQRKAGYAKKYALIGTRYG
                     SLDTKFRRIDTGETVDVPDGLAHFLEHKLFAEESGSIDDVFAELGAYSNAFTDYTMTA
                     YLFSCVDNFGPCLDTLLDFVMRPHFTEENVENEKGIIEQEIKRDEDSPAWQAMYGMLK
                     GMYQRFPVRLEVAGTVDSIRSIDVDILTRCYNTFYHPSNMTLLVVGDVDPNEVADRAS
                     AHLDDKLHGPVPAVERLYPHEPREVGMKRVERRMPVSEPLVCFGFKDNDVGYEGAQLL
                     RKTVCMEMLLYTLIGRSSALYNELYEAGLLDHTFDWNFDCEKDYSFVIFEGKSNSPDA
                     LVDRVCKGINDSIAAGIDRELFERARRSFIGSALRAFNSLEFIAYNFLAYHFRKMRLL
                     DRVGVAESISLDEVMDLGKQLFDPEMLVVSVVNPMQ"
     gene            2910..3578
                     /locus_tag="PLU15_04850"
     CDS             2910..3578
                     /locus_tag="PLU15_04850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO30282.1"
                     /translation="MTKLLLIRHGETMFNRARRLQGCKDVPLSDLGTRQADACANYIA
                     SLNRGTITALYSSPLARAYMTASAIGKAVGLDVVTDDRLKEIDVGALEGMTWDEVQLA
                     YPDFMVEHRADPIGTRYPGGESVADVSARARDLADGFVCDHGDETIVVVGHAIVLKAL
                     ICRVLNLDVANHLRLTVGNASLSIASVSRVDDDVKGKVLLLNDRHFLEALGLDAPRKE
                     GHEE"
     assembly_gap    3803..3902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4352..5512
                     /locus_tag="PLU15_04855"
     CDS             4352..5512
                     /locus_tag="PLU15_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOO30283.1"
                     /translation="MCGVSEAGRPRAREIGIHTGIFSPGEFNAITDVDGVMVGHCTVV
                     HGEGELVPGKGPARTGVTAIRPHGGNVFREKAPAAAYVFNGYGKSVGIHQINETGVLE
                     TPILLTNTLNVGLVADALIEWSVERNGDIGISTSTVNPVVGEVNDGYLNDIQGRHVRK
                     EHVYDALESAKTGLVEEGAVGAGTGSSCMGWKGGIGTASRVVPNSLGGYTIGVLVQSN
                     FGGVLTVNGAPVGRELGHYTYQEHFDNGSREQLDDGSVMVVVASDAPLDQRQLRRLAA
                     RASLGLARTGFYGSNGSGDFFIAFSTAFRVPHSEPATLSAEVVAGDAISALFFAAVEA
                     TEEAVLNSMFKARTVVGRDGHVVEAIDIGKLLGVMRRYNSLDWDKSLPPYIS"
     gene            6445..6705
                     /locus_tag="PLU15_04860"
     CDS             6445..6705
                     /locus_tag="PLU15_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO30284.1"
                     /translation="MTSAESPEVPVLVRQEWCKGCGICIAMCPKKVLEAAADGKVVVA
                     RPEDCIKCETCEIMCPDFAIRVVGGRSKEVARGREDVADARA"
     gene            6692..7834
                     /locus_tag="PLU15_04865"
     CDS             6692..7834
                     /locus_tag="PLU15_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOO30285.1"
                     /translation="MPGHNGRDLRLLQGNECCAEAAILAGCRFFAGYPITPSTEIAEL
                     LSEKLPQVGGKFIQMEDEIASMAAVIGASLTGAKSMTATSGPGFSLKQENIGFASFTE
                     VPCVIVNVQRTGPSTGLPTAPAQADLMQARWGTHGDHPIVALYPSTVPEVFDLTIKAF
                     NIAERLRVPVILLMDEVVGHMREKVAIPHQSEIEIVSRPQPDVPPEEYLPYAAGPDGV
                     PPMAPFGTGYRWHVTGLYHDETGFPTTAAPKVDRLMKRLYSKIESAADEITFTSDEFL
                     DDADTVVVACGITARSARRSVRLAREKGIRAGMVQLKTIWPFPYDLVGDIAGRVDRII
                     VPEMNMGQLIGEVERAAKGRCEVVGYSRVDGEPIAPAEIVAKIEEV"
     gene            7835..8662
                     /locus_tag="PLU15_04870"
     CDS             7835..8662
                     /locus_tag="PLU15_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOO30286.1"
                     /translation="MVAVPIDDYLRTQFMPHIWCPGCGHGIILGAVLRAIGRLDLPKD
                     EVCIVSGIGCASRAPGYLDFNTVHTTHGRALAFATGIKIARPDLHVIVLTGDGDAAAI
                     GGNHLIHAARRNVGLTTVMFNNSIYGMTNGQYSPMTPRGRYASTAPYGNVERNFDMCE
                     LVRAAGGNYVARATTAHARQLSNYVENAIQTPGFAFVEAVTQCPTYFGRRNKMGTPVD
                     MIEWFRKNSINAAAAAKLDPADLDGKIIIGEFYRGDAPEYTEVYDEVIERAKEAAAR"
     gene            8659..9204
                     /locus_tag="PLU15_04875"
     CDS             8659..9204
                     /locus_tag="PLU15_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO30287.1"
                     /translation="MRREIRLSGYGGQGLILAGIILAEAAMHDGHEVVQSQSYGPEAR
                     GGASRAEVIISDEPIDYPKVTNPDVLLLMSDQACKRFAGSACQGGTVIVDSGLITSIP
                     ETSGVVYKFPITDIAVEATGRSISANVAALGVLNTVAKLVSPESLKNAVSSRVPRGTA
                     EVNLAALRAGIAAAEAAGAER"
     gene            9264..10451
                     /locus_tag="PLU15_04880"
     CDS             9264..10451
                     /locus_tag="PLU15_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO30288.1"
                     /translation="MEKVAFATRMHDLGGDVVRNMFLLTENPDIISFAGGMPSPDALP
                     AEQLRKIADELMQDAGQQILQYSTIEGYTPLREWLASAVAEQGMKATTEQVLITSGAQ
                     QGIDLAAKCFINPGDCVLVERPTYLAALTIFKTYEVEFGCVDSDDYGIIPESFEEAIV
                     RHNAKMLYVVPTFQNPTGVTLPAERRKAIAEIAGKHGVIVVEDDPYAKLRYDGEPVPS
                     IASYDENDVVIFLGSFSKFISPGLRVGYAISPPEIHEKLAQGKQTTDFHTSNLSQRMI
                     YEFCTRGYLEPHLKSIRKDYAIRRDAALEALEKYFPEGTRWTHPQGGLFIWVVLPEHI
                     DTTELLPKAAAKNVAYIPGVPFYADGGGKNAMRLNFSNASLEQLDVGIKCLGEVISEA
                     MKA"
     gene            10561..12138
                     /locus_tag="PLU15_04885"
     CDS             10561..12138
                     /locus_tag="PLU15_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOO30289.1"
                     /translation="MAEFSPEKAYSAVRPSEFARQRWFRHKLSHVTNFEILDCAEFES
                     GVTADQCLVLGILRFEGAGDYFVPVVAESLLPGSGEKCPKAYGVIEDGSSGEKAVVYD
                     GAFHPMYVNTLLDLIESDATIPAVKGQFRFRKIISSRAERGWEISQVGDAYTNSVVIV
                     QRRSALKTFRVMTPGINPDVEVGIALATETDFRETPRVQGEVSYVDSCGVEYSVAVQT
                     EEVPNIGDAWELVEIALTEAMKSVAHGLKEGDAKFSDFDGILSADASRRLGATIGRFH
                     QAMASIDAPGFGVRRAGPGDIDMWRRNARARACGALADIRHALDCGLLADDADAAYAA
                     ERVLAAEESILEAAERMLWGLAPDLNGAGMVIRCHGDLHLGQILVRPDGSYAILDFEG
                     EPMASLEERRALHSPARDVAGMLRSYSYAANAAMAAARANCEAECLCKLESLVNEWES
                     RARTAFLEGYFGEACSGVGNLLPGDFRARCALVSYFELDKAMYEAGYELNNRPDWAVI
                     PLKGILQIMERRGGNTR"
     gene            12135..13301
                     /gene="glgA"
                     /locus_tag="PLU15_04890"
     CDS             12135..13301
                     /gene="glgA"
                     /locus_tag="PLU15_04890"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOO30290.1"
                     /translation="MKSVNSLGVTMMTREFPPNIYGGAGVHVDYLSTHLADLMNVEVR
                     CFGEGAQTEDAIRIRSYSPWLEMSKGSDPKLQKVLDPLSVDLAMVKDPIETEIVHCHT
                     WYTFFAGYLAKTMYGSKLVTTVHSLEPLRPWKEEQLGRGYSVSSWMERIGIENSDCVI
                     AVSKEMRQDVIEHYDVEPEKVVVIHNGIDLSGYRRTDDTSALEQYGIEEPYVLFVGRI
                     SRQKGIMYLLDAAPMIRPGVKVVLIAGTPDTKEIEEELAARVKDLPNVVWINRMLPRE
                     HVIQLYSHASVFACPSVYEPFGIINLEAMACEIPVVASAVGGIPEVVVDGETGFLVPP
                     GDPESLASAINRVIDCEKLRTQFGEAGRRRVERFFSWEAIAEKTKDMYLEILSE"
     gene            complement(13452..13832)
                     /locus_tag="PLU15_04895"
     CDS             complement(13452..13832)
                     /locus_tag="PLU15_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO30291.1"
                     /translation="MNERLINVGKRIREARLRQGMSQEELSEKCCMNPSYIGQIERGE
                     KSPSLNALYSIADGLDVNIRSFFESPETEEELAIQRLIDTIANKPPYQIDKIRRISEI
                     LLEDEVGMPSSTKPQTKGHSNPSR"
     gene            14081..14491
                     /locus_tag="PLU15_04900"
     CDS             14081..14491
                     /locus_tag="PLU15_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30292.1"
                     /translation="MPGQSEFEKIISAASLQVESISDNVYFGEGHDWKEIVRQLQEVA
                     GNLDIVLYGRNVPWSFYKRAQELRAEISYAINIANQRERYDKILELEEVLRSETSDAG
                     WRAYLSIDVLSLDPIVWEPVCMAACCLQRRHSLM"
     gene            complement(14580..14729)
                     /locus_tag="PLU15_04905"
     CDS             complement(14580..14729)
                     /locus_tag="PLU15_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30293.1"
                     /translation="MHTRKCQLQIEAALFTVMIIDTKLGKTSPETPVGIAEAGFQFQS
                     DVDYT"
     gene            complement(14808..15881)
                     /locus_tag="PLU15_04910"
     CDS             complement(14808..15881)
                     /locus_tag="PLU15_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOO30294.1"
                     /translation="MKVGFSGRPAPPREMRAPFGSTPLERMQRYVSACSEMELGCLEV
                     PATLVFGKGDWRNQFLSACTEPVRAEPKRTPSPVDVLVRVRFTDGVDPRAAESGPCTA
                     ATLTYARHLIRLFGGALVTLQFKPSHASLIGKAPVPTGESGAAKVGLVVDFADQSGVQ
                     AAARIVRKSRHYIMLPIVAITLDNMIKAERQIMRALTVCYNENGGGEIFVYLALPQSG
                     AAAGRVASRNWEAILETIANFECSRGSDVGLLFSGPGSEKHARAMAQMVSALYPPRCG
                     AGVESDLVTRIGSTVRIRDVELGLDMEYFIVAPEQASAKKGFLSSESPVGRAIIGQPR
                     GSTVQVAAPGGPILYELLEVLNN"
     gene            16068..18023
                     /locus_tag="PLU15_04915"
     CDS             16068..18023
                     /locus_tag="PLU15_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HOO30295.1"
                     /translation="MRCIERRLRVLIIMLTAFVCFSGSALADSPWVLRIAQGNGFVEI
                     ADGIVCVDNGPFVLMRSLAAGLGGDLTWIESTKESVFTTDRTVAVIKAGQRTAYINGQ
                     EVELSSLPDLSTGRIAISVKDLALLGLKAGVDFDLGTVTVAYQRTSVSSASFSVEDGR
                     VTIIVEADGGFECSDFILRQPDRVVVDIHDAVLSPSFQGFDVSYGAVERVRAAMNRPG
                     VVRVVVDLNAPLGYTISHEPGVPSRLAIRFNTLVGEIRLDQDTSIPRLRVKGDAPIEH
                     GSPVDDGGGCWHIDMKGTTAAGGITDLRDEIGPVNWVKASQPEPTTARLEFGLKKGYS
                     PIIRSPESNANELLIDFAITVSKMDVFEEPGCTVIQIVTSGPVEFKTFRLRSPERIGV
                     DLPGVWAGGYGSLPVESAGVEKIRFAQFTADVGRIVLDLQPEATRWAHSVEISADRTT
                     ATIRVGSSPIYGRTVMLDPGHGGSDPGTVHDGLYEKEINLDIALRARDLLVKAGATVV
                     MTREDDSTVPLGDRPRMANEVKPDAFLSIHCNSVLNVFPCGTETYYYNMIDYSQELAT
                     QIHYCLLKEIMLVDRKVRRKDYYVLKETQVPAVLVEVGYMSTSEENEKLRQPEFRQKA
                     AQGLVNGLAAYFDSDVYARWRTEVRAK"
     gene            18077..18928
                     /gene="murI"
                     /locus_tag="PLU15_04920"
     CDS             18077..18928
                     /gene="murI"
                     /locus_tag="PLU15_04920"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOO30296.1"
                     /translation="MQYRSVGVFDSGVGGLTVARAIMEVMPGQDIVYYGDTARMPYGD
                     KPPEEIVRYARQIISFLVERGVSAVAVACNTSSAIAVPVIGRDFDVPVVGMIQCGAAA
                     AAEASRSGRIGVIATANTVRSGAYSRAIANLRPCASVFEQACPMLVPMVERGEVSGPR
                     VREVVAGYLDPLLEAGIDTIVYGCTHYPFLEDEIRAVAGSSVALVDPAIKVASAVKTA
                     IGEDGQMVKMRGGLPSFVTSGDPCAFSQLVRSLLGWNVQEVWHVDLDAAEASAGKCSL
                     PGRADPE"
     gene            18949..19791
                     /locus_tag="PLU15_04925"
     CDS             18949..19791
                     /locus_tag="PLU15_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO30297.1"
                     /translation="MGRLELTIIGNQGPYSGPGGACSGYLVRSQGATVLIDCGPGVMP
                     RLQQHIDVSSLDAVVISHFHPDHYSDFLTMRYAFQYIESTGRMGCPVTLALPAVDAEW
                     FLREIIGEDYKPLFRIVPIEHGSRLEIGGRVRGGDCGLVLDFRLMSHAIPSFGTIVYA
                     SQPEHGSRAPLAYTSDTGLCESLEEMAASSHTLLVEASMQEGDAQRSSAGHLAAAEAG
                     RIASEHGVARVLLTHLHPAKLLERSVCEASEAYGGQVEIAAMGKTYALREQLAEEMED
                     ENEV"
     gene            19781..20533
                     /gene="rph"
                     /locus_tag="PLU15_04930"
     CDS             19781..20533
                     /gene="rph"
                     /locus_tag="PLU15_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOO30298.1"
                     /translation="MRFDGRTPEQMRPFKITRHYIKHAPGSVLVEMGDTRVLCAATYE
                     ERPPMHVKGSGRGWVTAEYAMLPRATIDRTPRERNTGRIAGRTQEIQRLIGRSLRSVV
                     DMASLGECTIWIDCDVIQADGGTRTASITGAYVALVDLLDSMDQWKGKPLPVSDFLSA
                     ISVGIVDGQLMLDLCYVEDSHASVDMNVVMTEGGKFVEVQGTAEGEPFARESLDSMLE
                     LAESGIRELCAHQRRALGELAERVGINGENNR"
     gene            20530..21129
                     /locus_tag="PLU15_04935"
     CDS             20530..21129
                     /locus_tag="PLU15_04935"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOO30299.1"
                     /translation="MKRLVVATSNRGKFEEIVHILSGLDISVESLSDYPGMVQAEETG
                     ETFRENALEKAIAAARHTHQVALADDSGLVVDALGGAPGVMSARYAGAGASDSDKYRK
                     LLLAMEGVPEGERTARFVCVVAIAAPDGRAWTAEGIVEGIITRVPRGNGGFGYDPVFL
                     VPELGLTFAEIDPETKNRISHRARALARSIPMVQEALGI"
     gene            21164..21240
                     /locus_tag="PLU15_04940"
     tRNA            21164..21240
                     /locus_tag="PLU15_04940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21198..21200,aa:Pro,seq:tgg)
     gene            21487..22476
                     /locus_tag="PLU15_04945"
     CDS             21487..22476
                     /locus_tag="PLU15_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOO30300.1"
                     /translation="MKRPNGEDGWYHVVHDLRPMRTVVGFFLYAELFGGGLRVTHKRA
                     KKVRLDALLVERGLVEDLKSARAWIMSGDVIVDDIREDKPGTLIRPEATIRMRGRNIP
                     FASRGGLKLSAALDAFRVDVSGRIILDAGASTGGFTDCLLSRGAARVYAVDVGYGQLT
                     GKLRQDPRVVNMERTNIGDIVLGQLDPVPDMGVIDLSYLSLSVAIPTVARLLAPSVPG
                     AEPEIIALIKPLFEVAASGPDLTRDQYRAAVERGLRAGEGIGWFARGVMASPVLGSGG
                     TLEFLAHYSLGKVHGDLDEKIDEAVCSGMQLVENAGSPHSRAGGMEESDEGNE"
     gene            22469..23488
                     /gene="pheS"
                     /locus_tag="PLU15_04950"
     CDS             22469..23488
                     /gene="pheS"
                     /locus_tag="PLU15_04950"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOO30301.1"
                     /translation="MNDKVEAVVERALSAIENAHTTAALEEARVRLLGRKGELTTLLR
                     QMKSVDPEDRPKFGALVNSARETIEKELDERARVLVAAEKAARLELERIDVTMPGRAP
                     RIGKIHPLTRGIMDITEIFVEMGFSVVEGPEVETDYYNFEALNTPKYHPARDVQDTFY
                     VNDTVLLRTQTSPMQVREMEKREPPVKVVVPGRVYRRDQVDATHSPVFHQIEGLLVDH
                     DITFTDLKGTLAMFARKFYGEERKVRFRPHYFPFTEPSAEMDISCAICGGAGCRVCKQ
                     EGWIEVLGCGLVHPNVLRAVGYDPDELTGYAFGMGVERLVMLKHGIDDLRLLYENDLR
                     FLSQF"
     gene            23514..25973
                     /gene="pheT"
                     /locus_tag="PLU15_04955"
     CDS             23514..25973
                     /gene="pheT"
                     /locus_tag="PLU15_04955"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOO30302.1"
                     /translation="MLVPLKWLSDYVDIDLCIDELADRLTMSGTKVETVTDIGDRLNR
                     IVAGKIVSVSPHESVGRLRVATVDLGPRQARRTLVTAAANIGRGDIVPVVLAGGTIAD
                     GTEIGEVDFSGVRSEGMLCSAKELGISSDGSGILRLPDDIKPGDDVVSALGLADTVIE
                     LEITPNRPDCLCMIGIAREVAAITGATLKMPNTALKEGSERAEELISVKIDAHDLCSR
                     YGARVIQKVSIGQSPIWMQVRLSAAGVRPINNVVDVTNYVMLEVNQPLHAFDYDRMSE
                     HAVIVRRARSGEVIVTLDGEERRLDPEMLVIADPGSALAVAGVMGGLSTEITEDTCTV
                     LLESATFARQSVWKTSRSLKLRTEASSRYEKGLDPETVPLALDRAGHLLEAIGAGVAA
                     SGIVDAYPSTAAVRVISTDAARISRRLGVDVSSDDVADSLGRLGFSVRVESVANKAKS
                     EGGSLVVEVPTFRGDVQEEIDLVEEVARMWGYDRIPTTVPAGAHVGGYTYEQEKAMQA
                     KTILTGAGASEALTIPFMSVADLDMLRVGQGDPLRDAIRIDNPMVEEQSYMRTTMLPS
                     MLVAIRTNINRGNRGVCLFELGKTYFPSQSLKRGEELRRGENPAVERQVLVCGMAGAY
                     TPRIWHTRERDYDFYDAKGMLEALLEGMGVDGATYVQTVRPSFHPGRTASIVWEDREL
                     GVIGEVHPDVSEAYGVAGRVYMFELDFARMLEAVDEAKSYEPLPRYPAIERDVAMVLP
                     RAVSIADVAELIEKTGGELVRSVTLFDVYEGLPVPEGKRSIAFTVTYRAQDRTLTDEE
                     VNRLHEEVRKALTDEMGAVLR"
     gene            26094..26372
                     /locus_tag="PLU15_04960"
     CDS             26094..26372
                     /locus_tag="PLU15_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOO30303.1"
                     /translation="MLTEQGPDGLDQEGVRVSAVILGEEYVIRGDTEAEQIESLAKEV
                     DLRMKAIAEANPRLSSQQVAVLSALNLMGELVVSKRRYSDLLDLLEQV"
     gene            26391..26960
                     /locus_tag="PLU15_04965"
     CDS             26391..26960
                     /locus_tag="PLU15_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOO30304.1"
                     /translation="MTVSSLDWLSIVLIVILVADFVVGYRRGFVRQLFDIAGIIIAIL
                     VAANRWQGASGLLQKWFPKLRPPGAHMVGFLALLIITLVVVDLIGQALSSAVSASGVS
                     ALNCFGGAALRILRSCLIISIVLVALVSLRIPSLTRTVESSPVAMKIIKVAPSIWDGF
                     EKFMPEQFRPSGPGGAKGEPAKPGGGGFI"
     gene            26923..28665
                     /gene="polX"
                     /locus_tag="PLU15_04970"
     CDS             26923..28665
                     /gene="polX"
                     /locus_tag="PLU15_04970"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOO30305.1"
                     /translation="MNQLNLAVAASFSEIADLLEIMGDNPFKVRAYRRAAEAIAGLDE
                     PASEILARDPGSIPGVGDALRAKVEEMESTGRIEYLEKLRLEVPAAILALIEVPGIGP
                     KTAWKLYESLGVEDEASLLAAARAGKIRDLPGFGEKTEDKIIRAIENMKSTEERRPLY
                     KVLPIAHDFEAMLGAVEGVERVGIGGSLRRRRDTVGDIDLIASTRAGDLVMEAFSASD
                     MLREITAMGPFKAEAIAVSGIRCEIRVVAPESYWTGFHHITGSRAHHVKLRRIAGRHG
                     LKISEYGVRRDDTGEMIPVGSEEELYAILGMDYIPPELREDWGEIEAALEGRLPDLID
                     EGAIRGDLHIHTNWSDGVASIEEMARAARDIGHEYIAITDHSRSLGIARGLSEDRLAE
                     HIEAVRRVDAKIDGIRILSGAEVDILKEGRLDYSDELLSKLDVVIASVHSGFQQDRDT
                     MTRRIIEAMRNPNVDILAHPTGRLLGRRPGYEVDIEAVIREAAATGTALEINSYPDRL
                     DLRDEHARMARDRGVMISINTDSHSPEELENIRYGVWVGRRAWLERQDVLNAMTCEEL
                     LVWLGSHKRHMFPV"
     gene            28763..29467
                     /locus_tag="PLU15_04975"
     CDS             28763..29467
                     /locus_tag="PLU15_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOO30306.1"
                     /translation="MFHAIVRSLTVYFFVLASMRLMGKREIGNISPFDLVVAIMIAEL
                     GAIPLQSHDIPLGHGLVPIAILAAVEVLVALACLKFPGIRRLVIGEPTVIIEDGKVMG
                     RNMQKLRYNINDLLSQLRDKDVFNVADVEFALLEPSGRLSVLLKSQRRPVTPEDLSIE
                     THYEGPSYPLVADGKIQYQYLKMLDLTVKDLTDMLKAKDIQSVEDVFFASLDSGGELY
                     VTMQDEVCLDELERGE"
     gene            29489..30046
                     /locus_tag="PLU15_04980"
     CDS             29489..30046
                     /locus_tag="PLU15_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30307.1"
                     /translation="MELSREFRERFVRSLQGRDYILYGGVLQLAKERGLRRISTRIIQ
                     IPSKDNSMYAVVEAEVETEDGVFSEVGDASPESVARSIQPHLLRMAATRAKARAMRDA
                     VGIDMVALEELGDALPDRDVMDEEVDLRSPEDLVIRFGKYSGKTLGEIYGVDRGYVEW
                     LSQNARDEAIRDASRELMLPSTPEM"
     gene            30150..>32656
                     /locus_tag="PLU15_04985"
     CDS             30150..>32656
                     /locus_tag="PLU15_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOO30308.1"
                     /translation="MDDRSLRVLEYYKVRDMLEQCASCELGRERIRQLAPITNLAMIE
                     EAQRGTSEARSLLDMGKQIPFGGVRDVRSSVERADRGATLSCDELLDILSTLSTSRSL
                     KKILGSLGPEYPVLADAAADIGTFGDLEQAISRAISESGEVMDSASSALRSIRSRIRS
                     LNAKVRDNLDSMVRSPQWSRLLQDPIVSIRNGRFVVPVRAEMRSQVPGIVHDQSASGA
                     TVFVEPMPVVELNNELRQAISQEEAEVERVLSELSAKVGSQCEQLRLTLDAIGNIDFI
                     MAKGRLSQRMGASVPQLNDMGHINLIAARHPLLKGDVVPIDAYLGQTFTTLVITGPNT
                     GGKTVTLKTIGLITLMAQSGLHIPAEPGSDVAVFSKVFADIGDEQSIEQSLSTFSSHM
                     THIVAIMSMVDHDSLVLLDELGAGTDPQEGAALAMAILETLHLTGARTVATTHYSELK
                     IFAHTTEGLSNASVEFDVETLCPTYRLSIGVPGASNAFAIAERLGLPEEVLDRARSLV
                     GTGGQRMELMITNLKEECDRAETAREDAEKLRSRYLRLKDEYEEQTRRLRSERSEIMR
                     AARAEAESIIQEARKESERIIGRLRAIQAQQELVAKRLVEEEVITEDSLPRSAVCGEA
                     SLPDEAIRAARRDLEALREITRELTAVAPESDGEVTTIADVHDDGWSSRIEQGDDVEV
                     VALGQKGVVLDQLGPDEYMIGIGPMRVNVNRGSIRKLRSSQAEEPDRGYGRRGTMREP
                     GSVSFETQKAATIGAELDLRGMRVEEALFAVDKYLDDAVLAGIQKARIIHGKGTGALR
                     DAVTEQLGSDPRVASFRFGLAGEGGDGVTVVSFGTPGA"
BASE COUNT         7537 a         7432 c        10019 g         7568 t
ORIGIN      
        1 ggagaatatt gagattccag tgcagtgata caactcgatc tttgaggctg tgcatgcttc
       61 tgtggttgcc atgtgggcag gcatagcaga ttatgagccc ctttttgcgt gtcttgctgt
      121 tgttgatgct ctgatgcacg caattgatgg gaatgtacaa gcttgccgtc tggggatcct
      181 ccaggaatat gcggttgccg gcttgaactt gcgaatgaat atgggtcata ctaagaatca
      241 gtgggaattg ctttttctcc ggagaatgcc ttgcggtgca agaacacagg ggggttcgat
      301 cattggacga atggttttct acggatgtgt tgccgaatgg gataaacgtc tacgtaatgc
      361 aaacctctaa gtttaagagc aacacaatta ccgcggtgat ttactctgac ctgactgagg
      421 atacagtcac tcttaacgct attctgccag aagtgctgag caggggcacg aggcgctggc
      481 caaggactgc cgagttcagg cagaatcttg acagcctgta tgggacaggc ttgtctgccg
      541 gtgttagtaa gaggggtgag aagcagctcc ttatcttttc tgttgatgtg gcgaatgacg
      601 cgtatttgtc ggcagatgaa gacctgttgc gccagggaat cgggatattg cgagacgtgg
      661 ttttcgatcc ggttctggaa gctgaggcat ttcgcgagga ttatgtggaa caggaaaaga
      721 tcaatcttag gcggcgaatc gagagcataa tagataacaa gatgggttat gccttcaaaa
      781 gatgcaacga agagatgttc agaggcgaac cgtatgccct gtacaggctg ggtcgggttg
      841 aggatattcc tggcattgat cctgaaggcc ttcgctcttg gcacaaaaaa gcggttgcgc
      901 agctcccatt ggacattttt ctagtgggag atgtggcccg cgaacgcgtg ttggaactgg
      961 tgggagaggt tttcgatatg gagcgcaaac ctatgccgct tgaggtgcct gccagggttg
     1021 ggcatcgagt gcctgtacag ccgagacagg ttgtagagag catggatgtg aatcaaggaa
     1081 gcctggtcat tggctattgg actggcgtca acatgtccga ccccgactat ccagcgattt
     1141 tgatggccaa tggcatctac ggagggtatg cgcactcgaa actctttcag aatgtccgcg
     1201 aaggtgaaag cttggcctac tccgtctatt ctggaattga tcagaataag ggcgtcatga
     1261 atgtttgggc ggggatagcg cctgaagcca tggataaggc ggtcgagata atcgaggagc
     1321 agttagatga tgtgaggtct ggcaggatct ccgatgacga aatggacaag actcgcaggg
     1381 caattacgag agacatacgc atgatggggg acagtccggg cgctcagatt gattacgcca
     1441 tcgtctccag gataaacggc gttggggaga aacctgacga gctattgcgg cgcatagaag
     1501 cagtttcctt ggatgacata gtccgtgttg cgagcaaaat agagcttgac acaatctact
     1561 tcctgcgcaa tgaggaggac gaggctggtg cgtagtatgg aaattgcaag gtcggaactg
     1621 ctccgggagg aagtatccac tacccgtctc gactgcggcc tggaagtgtg cgtccagcgg
     1681 aaggcggggt acgcaaagaa atacgccctt attggcacga gatatggatc gctcgacacc
     1741 aagttccgca ggatcgatac gggagaaact gtggatgtcc ctgacggatt agcccatttt
     1801 ctcgagcaca agctttttgc ggaggagtca ggatcgattg atgacgtctt tgcggagctg
     1861 ggtgcctact caaacgcttt cacagactat acaatgactg cctacttatt ttcctgtgtc
     1921 gacaatttcg ggccttgtct cgataccctc ctggattttg tgatgaggcc tcatttcact
     1981 gaggagaatg tggagaatga gaaaggcata attgagcagg agatcaagag ggatgaagac
     2041 tcccccgctt ggcaggcgat gtacggaatg ctgaaaggca tgtatcagcg tttccctgtc
     2101 cgcttggagg ttgcggggac agtcgattca atacgctcta tcgacgtgga cattctgaca
     2161 aggtgttaca atacgttcta tcatccgtcc aacatgacat tgcttgttgt cggcgacgtt
     2221 gatccgaacg aggtggctga ccgcgcctcg gcgcatctgg atgataagct tcacgggcca
     2281 gtgccagcag tagaacgtct gtatccgcat gaaccgcgag aagttgggat gaaacgtgtg
     2341 gagcgcagaa tgccggtgtc ggagccattg gtctgttttg gattcaagga taacgatgtg
     2401 gggtatgaag gcgctcaact tctgcgcaaa actgtgtgca tggaaatgtt gctttacact
     2461 ttgatagggc gcagctccgc actgtacaac gaactttacg aagcagggtt gctcgaccac
     2521 acgttcgact ggaatttcga ttgcgaaaag gactacagtt ttgtgatatt cgaggggaag
     2581 agcaattctc cggatgctct ggtggaccgc gtttgtaagg ggataaatga ctccattgcg
     2641 gcaggcatcg acagagagct gtttgagcgc gctcgccgga gtttcatagg ctctgcgctc
     2701 agagcattca actctttgga gttcatagcc tacaactttc ttgcttacca cttcagaaag
     2761 atgagattgc ttgacagagt cggggttgct gagagcattt ccctggacga agtgatggat
     2821 ctcggcaagc agctgttcga tcccgagatg cttgtagtat ccgtggtgaa tcccatgcaa
     2881 tagagtttct ggcaatgagg gggacaaaca tgacgaaact gcttttgatc agacacggag
     2941 agactatgtt caatcgcgca aggcgattgc agggatgcaa ggatgttccc ctctcagatc
     3001 tcgggacccg gcaggcggac gcctgtgcta actacatagc ttccttaaat cgcggcacga
     3061 ttacagctct atactcgagt cctcttgcca gggcgtacat gactgctagc gccattggca
     3121 aggcggtcgg cttagatgtg gtgaccgacg atcgactcaa agaaatcgat gttggcgccc
     3181 tcgaaggaat gacctgggac gaggtgcagc tggcttaccc ggatttcatg gtcgaacata
     3241 gagctgatcc cattgggacc aggtatcctg gaggagaaag cgtcgcggat gtctcggcgc
     3301 gagcacgcga tcttgccgac ggatttgtct gcgatcatgg cgatgagaca atcgtcgtcg
     3361 ttggacatgc tattgttctg aaagctctga tttgccgcgt gctcaatctg gatgttgcga
     3421 atcacttgcg gctcacagtg ggcaatgcgt ctttgagcat tgcaagtgtt tctcgggtgg
     3481 atgacgacgt aaagggtaag gttttactgc tcaacgatag gcacttcctc gaagcattag
     3541 gacttgacgc gccgcggaaa gaggggcatg aggaatgaag gctgtgttat gaaaactgtg
     3601 tgtcccttgg tcgatacctg tggacttatg gcaatggtgt cggaggtcgt gtagtcaaaa
     3661 taaagggaga tcccggccac tcaattatgc gtgagttcat agacaagaac tccgccgtga
     3721 caaaatatcc acatgcttcc gcttatccgg gaggcgtgat gattgggcca aggttgtgct
     3781 catttacgga atcgtcgtgc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nncgggaggc gtgatgattg ggccaaggtt gtgctcattt acggaatcgt cgtgcggctc
     3961 agcgtgggga cggatggaac gttggcgctc gtcatggctg ccgtcatcac caaggacggt
     4021 tttgttaatc atccatagca gtgttcgtgg gtttgattga tttgcggtgc ggatccgttg
     4081 cgacggagat atggtgcgct tcgctgtgca ttaaggcgag gtgctttttc ggctaggctc
     4141 gacagtcgat ccagaccgga tgtggtcgtg gcttacaacg gtgagcctgt caggtgtggg
     4201 ggaggggtca gctttctcat aggagaacac gtgggcgata tcgggcttta ggccgcattt
     4261 tacgattgcg ccttcagtgt tgaggaagca caagaatcag cagagaagtg aaagtgcaaa
     4321 actgagaatc cacgggcagg tgatgttttc gatgtgtggc gtttcagaag caggtaggcc
     4381 tagggccagg gagataggga tacacacggg catcttttcg cccggagagt ttaatgccat
     4441 tacagatgtg gatggggtca tggttggcca ttgcactgta gtgcacggcg agggcgagct
     4501 tgttcccggg aagggtcctg ccagaacagg agtgacggca attcgtcccc atgggggcaa
     4561 tgttttccgt gagaaggcgc ctgcggcagc ctatgttttc aatggctatg gcaagtccgt
     4621 aggcattcac caaattaacg agacaggtgt gctggaaacg cctatattgc tcacgaatac
     4681 tctgaacgta ggtcttgtcg ccgatgcatt gattgaatgg tctgttgagc gaaatggtga
     4741 tatcggcatc tcaacgagta ctgtcaaccc cgtggttggg gaggtgaatg acggctactt
     4801 gaacgatatt cagggaaggc atgtccggaa ggaacatgtg tacgatgccc ttgaatctgc
     4861 gaagactgga ctggtcgaag agggagcagt gggcgcaggc acaggaagca gttgcatggg
     4921 gtggaaaggc ggaattggca cggcgtccag agtcgtcccg aacagcctgg gcggatacac
     4981 catcggagtg ttggtccagt ccaacttcgg cggagtcctt acggtaaacg gagcgcctgt
     5041 cgggcgggag ctcggacatt atacctatca ggagcatttc gacaatggct ctcgggaaca
     5101 acttgatgat ggctctgtta tggtggttgt agcttctgat gccccattgg accagcggca
     5161 gctgaggcga ctggcggcaa gggccagcct cggccttgcc cgaacagggt tctatggaag
     5221 caatgggtca ggcgatttct tcatagcctt ctcaacagcc ttccgtgttc cacattcaga
     5281 gcctgcaacg ctcagtgcag aggtcgtcgc gggcgatgcg atatccgcac tcttttttgc
     5341 agcggttgag gcgactgaag aggctgttct caactcgatg ttcaaggcca gaacagtggt
     5401 agggcgcgac ggacatgtcg ttgaagccat agacataggt aagcttctcg gtgtcatgcg
     5461 cagatacaac tcgctagatt gggataagtc actgccgcca tatatctctt aacgcatgcg
     5521 ttaagacggg aaacatcatg ggaagttgaa aaagaggagc ttacgggcga aagcggcctc
     5581 ttcttcttta tgctcgctat ggtccaaaga atcgcatgca actgatgttg tctttacagg
     5641 ctaggccttg cggagagcac ccaagtacta tgacaaattg gcttatgtta tttagcattg
     5701 ttactgtggt gttggcagcc aaggcaggtt catacaagat tgcatatgtc cgcatcaaag
     5761 tgatagaatt gaagaggtga agtatacagt tgttctcatt cctcggttgc gtagtgaatg
     5821 gggccttttg catgaccttg ccattgtcac atgtaggcag gcaaaaacga gtttgggggt
     5881 aggctggcct atggccgtaa ctgccgctgc tgacgcgagg cgaagaaaaa tgaaatggtt
     5941 gatgccctgg actggtttga aagtaaaaat gattcccgtt tatcccgtac agaaggcaat
     6001 cggttttcct ggcctaggta aacacgggga tgttggggcg ttaatcgcca gtgcacaagg
     6061 ggttactgag atgatcgaat gcccggacgc atgtgcaacc gaattgagga tgacttgcag
     6121 gattgtacgc tataagatac agaggcgttc tgtctgtggc tgtgaccttt ccttgtctgt
     6181 ttggcccgca ctcgtttgcg ggctgtcttt atttctgtga ggatttgttg ttggatcgaa
     6241 ctgcgtgaac atcgtgaaca tatggaatga tgaacgctgg caggcgatgt cctgaacatg
     6301 ttctgcagct atatgccgga tagggtagag cgccccttgg ctgttggcaa aagctgtcgc
     6361 agagggctgc tcggccgaga tcccacatcc gagttgcaga cattcgttct cggccaacta
     6421 accaaaagat gtggagggga gtccatgact tctgcggaaa gccccgaggt tccagtcctc
     6481 gtgaggcagg aatggtgtaa gggctgtggt atatgcatcg ccatgtgccc aaagaaagtt
     6541 cttgaggcag cggcagacgg aaaagttgtt gtagcaagac ctgaagattg tatcaagtgc
     6601 gaaacctgtg agattatgtg ccctgatttt gccatacgtg tggtgggcgg cagatccaag
     6661 gaagttgcac ggggaaggga ggatgttgcg gatgcccggg cataatggca gggatcttag
     6721 gctattgcaa ggcaatgaat gctgcgcgga agctgcgatt ctcgcaggat gcaggttctt
     6781 tgcaggctat ccgataacgc cgtctacgga gattgccgag cttctctcgg aaaagctccc
     6841 acaggtcggc gggaagttca tacagatgga agacgaaata gccagcatgg cagcggttat
     6901 aggggcttca ctcactggtg ccaagtcaat gacggctact tctggtcccg gcttttctct
     6961 gaaacaggag aatatcgggt ttgcttcatt caccgaggtt ccatgcgtaa tagtgaatgt
     7021 tcagcgcacg ggaccgagca cgggcttgcc tactgccccg gcgcaagcgg atctgatgca
     7081 ggcgagatgg ggaacgcacg gtgaccatcc catagttgcc ctctacccat ccactgttcc
     7141 tgaggtattt gaccttacga tcaaggcgtt caacatagcg gagcggctca gggtaccggt
     7201 tattcttctc atggacgaag ttgtggggca catgagggaa aaagtggcga ttcctcatca
     7261 gtctgagatc gagatagtta gcaggcctca gcccgatgtt ccgcctgagg aatacttgcc
     7321 atatgcggca ggccctgacg gcgtgcctcc tatggcgcca tttggaacgg gatacaggtg
     7381 gcatgttacg ggattgtatc atgacgagac cgggtttcca acaacggcag cgccgaaagt
     7441 tgaccgactt atgaagcggc tttactcaaa gatagagtct gccgcggatg aaatcacatt
     7501 cacatcagac gagttcctgg acgatgcaga tactgttgtt gttgcttgtg gcataactgc
     7561 tagatccgct cgccgaagtg taaggcttgc acgtgaaaag gggattaggg caggaatggt
     7621 tcaactgaag acgatttggc cgttcccata cgacctcgtt ggcgatatcg caggcagggt
     7681 tgacagaatc atagtgcctg agatgaacat ggggcaatta atcggtgaag ttgagcgtgc
     7741 agccaagggc aggtgcgagg ttgtgggtta ctcccgagtt gacggcgaac ccatagcccc
     7801 agctgaaata gtagccaaga tagaggaggt gtgactggtg gcagtgccta ttgatgatta
     7861 tctacggact caattcatgc cgcatatctg gtgcccagga tgcggacacg gcattattct
     7921 cggcgctgtt ctgagagcca tcggcaggtt ggatcttccc aaggacgaag tgtgcatagt
     7981 gtcagggata ggctgtgctt ctcgtgctcc cgggtatctg gatttcaaca cggttcacac
     8041 aacacacggg cgggctctgg catttgccac tggaataaag atcgccaggc cggatctgca
     8101 tgtgatagtt ctcacaggag acggcgatgc tgccgccatc ggcggcaacc atctgatcca
     8161 cgcggccagg aggaacgtcg gcctaaccac tgtcatgttc aacaactcga tctatggcat
     8221 gaccaatggt cagtattcgc ctatgacccc taggggaaga tacgctagca ctgctccata
     8281 tggcaacgtg gaacgaaatt tcgacatgtg cgagctcgtc agggccgctg gcggcaatta
     8341 tgttgctcgg gccacgacag cccacgccag gcagttgtcg aactacgtgg agaacgctat
     8401 tcagactcca ggatttgcgt tcgttgaggc tgtcacccag tgtcccacat actttggacg
     8461 aaggaacaag atggggaccc ctgttgacat gatagagtgg ttccggaaaa actccattaa
     8521 tgcagcagcc gctgcaaagc tggacccagc tgatctagac ggaaagataa taataggtga
     8581 gttttatcga ggagacgccc ccgagtacac cgaggtgtac gacgaagtaa tcgagcgggc
     8641 aaaggaggcg gccgccagat gagacgtgag attaggctct caggctatgg tggacagggg
     8701 ctgattctgg ccggaataat cttggcagag gctgcaatgc acgacggcca tgaggtagtt
     8761 cagagtcaga gctatggacc ggaggcacgc ggcggcgctt ccagagcaga ggttatcatt
     8821 agcgacgagc cgatagacta tccgaaagtg acaaaccccg atgtgcttct tctcatgtca
     8881 gaccaggcat gcaaacgctt tgcgggaagc gcttgccagg gaggaacagt aatagtcgac
     8941 tctggactta tcacttccat tccggagact tccggggtcg tttacaagtt ccccattacc
     9001 gacattgctg tggaagctac aggcagatca ataagcgcca acgtggcagc tctgggcgta
     9061 ctgaatactg tggccaagtt ggtttctccg gaatcgttga aaaacgcggt ttcgtctaga
     9121 gtgcctcggg gcactgccga ggtcaacctg gctgcattga gagcagggat agctgccgct
     9181 gaggctgcag gtgcagaaag ataggtggag aatactagat ttgggccgac atgatccaac
     9241 atagttagga gagtgatgat ccattggaga aggttgcatt cgcaactagg atgcacgatc
     9301 tcggcggtga tgtcgttcgc aacatgtttt tgctcaccga gaacccggac atcatctctt
     9361 ttgcaggggg tatgccctcg ccagacgcac tccctgctga gcagcttcgc aagatagccg
     9421 atgagctcat gcaagatgct ggccagcaga tattgcagta ttcgacgatc gagggctata
     9481 caccgctcag agaatggctt gcaagcgccg ttgctgaaca ggggatgaag gcgaccactg
     9541 aacaagtgtt aatcacttca ggagcgcaac aaggcatcga tttggccgcg aaatgcttca
     9601 ttaaccctgg cgactgcgtg ttggtggaac gacctacgta tctagcagca ctcacaatat
     9661 tcaaaacgta tgaagtggaa tttggttgtg tggattcgga tgactatgga ataattccgg
     9721 agtctttcga agaagcgata gtgcgccaca atgcaaaaat gttgtatgtg gttcctacgt
     9781 tccaaaaccc gaccggagtt acgttaccag ctgaacgaag aaaagccatt gctgaaatcg
     9841 ccggcaagca tggcgtgatc gtggttgaag acgaccccta tgctaagttg agatacgatg
     9901 gggagccggt tccctcaata gcaagctatg acgagaatga tgtcgtcata ttcctaggga
     9961 gcttttcgaa gttcatctcg ccgggacttc gcgtgggata tgcgatatcg cccccggaga
    10021 ttcacgagaa attggctcag ggcaagcaga caactgattt ccacacttca aacctgtctc
    10081 agaggatgat ctacgagttc tgtactcgcg ggtatctcga gccgcatctg aagtcgatca
    10141 gaaaggacta cgcaatcagg cgcgacgcag cgctggaggc cctcgaaaag tatttccctg
    10201 aggggactcg atggacgcat ccacaaggtg gccttttcat ttgggttgtg cttcctgagc
    10261 atattgatac cactgaactg ctgccgaagg cggctgcgaa gaacgtagcc tacatacctg
    10321 gagtgccatt ttatgcagac ggtggtggga agaacgccat gcgtctcaac ttcagcaatg
    10381 caagcttgga gcagcttgat gtgggaatca agtgccttgg tgaggttatt tcagaagcaa
    10441 tgaaggcgta aaacgctgct gagggaaaga ctagatcttg aagagctatg gacccgggtg
    10501 cgcccgcccg ccggacggga tgtgtccggg tcctttgcaa ttaggcggag gtgtattact
    10561 ttggcggaat tctcgccaga gaaagcatat tcggcggtca gaccctcgga atttgcgcgt
    10621 cagcggtggt ttcgccataa actgtcacat gtgaccaatt ttgagattct cgattgcgcc
    10681 gaatttgagt ccggtgtaac tgccgatcaa tgcctggttc ttggaatctt gcgttttgaa
    10741 ggagcaggcg actattttgt tcctgtggtc gcagaaagtc tcctgccggg ttctggagaa
    10801 aagtgcccta aggcttatgg cgtaattgag gatggatcct cgggagagaa agctgttgtt
    10861 tacgacggag cttttcatcc catgtatgtc aacacactac tggatctcat agaatcagac
    10921 gccactatcc cagctgtgaa agggcagttt cgattcagaa aaatcatcag cagccgggcc
    10981 gagcggggct gggagataag ccaagtagga gatgcctata ccaacagcgt ggtgattgtc
    11041 cagcgccgaa gcgcgctcaa gacgttcagg gtcatgactc cggggatcaa tccagatgtg
    11101 gaagttggga ttgcccttgc aaccgaaacg gattttcgtg agaccccccg ggtccaaggg
    11161 gaagtctcct acgtggatag ctgtggcgtg gagtattctg tggccgtgca gacagaggaa
    11221 gtcccgaaca taggggatgc ttgggagctt gttgagattg ctctgactga agcgatgaag
    11281 tcggttgcgc atggactaaa agagggagat gccaagttct cggatttcga cgggattctg
    11341 tctgcggatg catcgaggcg tcttggcgca actattggca ggtttcatca ggctatggct
    11401 tcaatagatg ctccgggctt tggcgtgcgg cgcgcgggac caggcgatat cgatatgtgg
    11461 agacgaaatg cacgagcgcg cgcgtgtggg gctttggctg atattagaca cgccttggac
    11521 tgtggtttgt tggcggacga tgctgatgcg gcttatgcgg cagagcgcgt gctggcggct
    11581 gaagaatcga tactcgaggc agcggaaagg atgctatggg gactcgcgcc tgatctgaac
    11641 ggggcaggaa tggtcattag atgccatggc gaccttcatc ttgggcagat tcttgtgcgg
    11701 ccggatgggt cttatgctat cctcgatttt gagggcgaac ccatggcttc gctggaagag
    11761 aggcgcgcct tgcacagccc ggcccgggat gtggccggca tgctaaggtc gtacagctat
    11821 gcagcgaatg ctgctatggc agctgcccgg gcgaattgcg aagcggaatg cctgtgcaag
    11881 ctagagagtc tcgttaatga atgggagagc cgtgcgagga ccgcatttct tgagggatac
    11941 ttcggtgagg cgtgttctgg ggtcggcaat ttgcttccag gggattttcg cgcacgatgt
    12001 gccttagtat cctatttcga attggacaag gccatgtatg aggctgggta tgagctgaac
    12061 aacaggcctg attgggccgt gatacccctc aagggtattc tccagatcat ggaaagacgg
    12121 ggaggcaaca caagatgaag agcgtcaact cgcttggggt tacgatgatg acgcgtgagt
    12181 ttccgccgaa catatacggt ggggcaggag tccacgtcga ctatctttcg acgcatcttg
    12241 cagacctcat gaatgtggag gtgaggtgct tcggcgaggg ggctcagacg gaagatgcga
    12301 tacggattcg cagttacagc ccctggctgg agatgtccaa aggatccgat ccgaagctgc
    12361 aaaaggtgtt ggatccgctt tcagtcgatc tggcaatggt aaaggacccc attgagacag
    12421 agatagtcca ctgccatacg tggtacacgt tttttgcggg ttatcttgca aagacgatgt
    12481 atggctcgaa gctcgttact acagtgcatt cccttgagcc gctccgccca tggaaagagg
    12541 agcaattggg cagaggatat tctgtcagct cgtggatgga gaggatcggg atagaaaact
    12601 ccgattgcgt tattgcagtc tcaaaagaaa tgcgtcagga cgtgattgag cactacgacg
    12661 tggaacctga gaaggttgtt gtgatacaca acgggattga ccttagcggc tatcggcgga
    12721 ccgacgatac tagcgctctg gagcagtatg ggatagagga accttacgtg ctcttcgttg
    12781 gaagaatatc gagacagaaa gggattatgt atctgcttga cgctgctccg atgataaggc
    12841 caggagtgaa agtcgtgctg attgcgggca ctcctgacac aaaagaaata gaagaggaac
    12901 ttgctgcgcg cgtaaaagat cttccaaatg ttgtatggat caacaggatg ctcccgcgtg
    12961 aacacgtaat tcaactctat agccacgcgt cagtatttgc atgcccgtct gtgtacgagc
    13021 ctttcgggat tataaacctg gaagccatgg catgtgagat tcctgtcgta gcatcagcag
    13081 taggtggaat ccccgaagtg gttgtagacg gggaaactgg tttccttgtt ccaccgggag
    13141 atcctgagag cctcgcgtcg gcaataaacc gtgtcatcga ttgtgaaaaa ctccgcactc
    13201 aatttggcga ggctggaagg cgccgcgttg agcgcttctt ctcttgggaa gcaattgcag
    13261 agaagacgaa ggatatgtac ctggaaatcc tgagcgagtg aagcgctgag ggggagaaga
    13321 aaaactgctc atcagagtct aattgaggac gggctttatt aggtagaatt ggcgcggatg
    13381 aaaaaagaaa aaagcctggg ttatgtcatg gcccaggcta gttttgttgt ggacaatgtg
    13441 tgggaagacc atcaacggct agggttgctg tggcctttgg tctggggctt agtgctagaa
    13501 ggcattccaa cctcatcctc gagcaagatt tccgagattc tcctgatctt gtcgatctgg
    13561 tatggaggct tatttgctat cgtgtcaatg aggcgctgga ttgccaattc ctcttcagtt
    13621 tcgggcgatt cgaaaaaaga tctgatgttt acatccagac cgtcagcaat ggaatacaga
    13681 gcattgagtg acggactttt ttcaccccgt tcaatctgtc cgatgtagct agggttcatg
    13741 cagcacttct cggacaattc ctcctgcgac atgccctgcc gaagccttgc ttcccgtatt
    13801 cgttttccta cgtttatcaa tcgttcgttc aatttatcca ttccccatga tgatggtaac
    13861 tcggacaaga atctggcgga agcagctggc agcacatatt gctggcaaaa aaagtgccac
    13921 aggcacctac ggcctgcttg aagcgatatg acagaactag taaggaaagg gctaaaatca
    13981 ctccaaatat catacatggg tattaggaag gaggcgatac aagattatat cgccttgtga
    14041 taggatcggc atgaaagaat tggagacggg aggaattcaa gtgcctgggc agtccgagtt
    14101 tgaaaagata atcagtgcgg catcacttca agtagagagc attagtgata atgtctactt
    14161 tggcgaaggc catgactgga aggagattgt acgccaattg caggaggtgg cggggaacct
    14221 tgacatagtt ttgtacggtc gcaacgtgcc ttggagcttc tacaagcgtg cacaggaact
    14281 gcgggcagag atctcctacg cgatcaacat agctaaccag cgcgagcgct atgataagat
    14341 cctggaactt gaggaggtgc tcaggtcgga aacgtccgat gcagggtggc gtgcttatct
    14401 cagcatagat gtgctctctt tggatccgat tgtttgggag ccagtgtgca tggcagcgtg
    14461 ttgcttgcaa agaaggcata gtcttatgtg aactgtcgaa tacaactgcc ggatgaccgc
    14521 cgcataacca tggccgaagg caagccatgt ctgagatgcg caagtccagt cagatctcct
    14581 tatgtgtaat caacgtcaga ctgaaactga aatcccgctt cagcgatgcc caccggtgtt
    14641 tccggcgagg tctttccgag cttggtatcg atgatcatga ccgtaaacaa ggccgcttcg
    14701 atctgcaact ggcactttcg agtatgcaca aatcccctga gccaggcgtt cattctcgcg
    14761 ttgcttgcct atggcttgtt tagcctggcg ggcaagatct agctttgtca gttgttgagc
    14821 acttctagca gttcatatag tatcggcccg cctggcgccg ccacttgcac tgtggagccg
    14881 cgaggttgtc cgattattgc acgtcctact ggtgattcgc tagagaggaa ccccttcttg
    14941 gctgacgctt gttcaggcgc aacgatgaag tattccatgt ccaatccgag ctcgacatcg
    15001 cgtattctta ctgttgagcc tatgcgcgtt acgaggtcag attcgacgcc agctccgcaa
    15061 cggggcgggt aaagggcgga gaccatctga gccattgcgc gcgcatgttt ttccgatcca
    15121 ggaccggaaa aaagcaagcc cacatcagag cctctagagc attcgaaatt agctatcgtt
    15181 tccaaaatgg cttcccaatt acgagaagct acacgaccag ctgctgcgcc agactgtgga
    15241 agcgcaaggt agacaaagat ctcgcctcct ccgttttcgt tatagcatac tgttagtgcc
    15301 cgcattattt gacgctctgc cttgatcata ttgtccaagg tgatcgccac tatgggcagc
    15361 attatatagt gtctcgattt cctgacgatt ctggcagctg cttgcactcc tgattggtca
    15421 gcgaagtcaa cgaccagccc gacttttgca gctccggatt ctccggtggg aactggggct
    15481 tttccaataa gagaagcatg ggatggcttg aactgcaatg tgacaagagc gccgccgaac
    15541 aggcgtatta ggtgcctggc gtatgttagg gtagcagctg tgcacggacc ggactccgct
    15601 gcccgaggat cgactccgtc agtgaacctg acacgtacga ggacatctac gggcgaagga
    15661 gttctcttgg gttctgcgcg aactggctct gtgcacgcag agagaaattg attgcgccag
    15721 tctcctttgc caaacacgag ggtggctggc acttccaaac atccaagctc catctcggag
    15781 caggccgata catatcgttg catccgctct agtggcgttg acccgaaagg cgcccgcatt
    15841 tctctgggcg gcgccggcct tcctgaaaac ccaactttca tttttgtcgc ctcctttagc
    15901 ctatgatgtc cagctgaaat gagaaatagt catttgaatc gcacagtttt gtatctgtgg
    15961 ttccacgatt gccaagcgtc cagaagcgat tgatcgtggg tgcagaggat tatgccgaaa
    16021 agagtagaaa aaagtgattg aggacgttca cagagggggc tgtgcgattg cgttgcatcg
    16081 agaggcgctt gagggtgttg atcatcatgc tcacagcgtt cgtctgtttt tcaggatctg
    16141 cgttagccga cagtccatgg gtccttcgaa ttgcgcaagg caatggcttt gtcgaaatag
    16201 cggacggcat agtctgcgtt gacaacggac cattcgtgct catgcgttct ctcgccgcgg
    16261 gccttggagg agatctaacc tggatagaat cgacaaaaga atcggtgttt actacggaca
    16321 gaactgttgc ggtgataaag gcgggacaaa ggactgctta catcaacggg caggaagttg
    16381 agctctcgtc gctgcctgac ctgtccaccg ggcgaatagc gataagcgtc aaggacctgg
    16441 cgttgctcgg cttgaaagca ggcgtcgatt tcgatctcgg gacggtcaca gttgcctacc
    16501 agagaacttc tgtttcatcc gcttcgtttt cggtggaaga tggaagagtg acgatcattg
    16561 tcgaggcgga cggcggtttt gaatgctctg acttcattct gcgtcaaccg gatcgggtgg
    16621 tagtggacat acatgatgct gtgctgtccc cgtcctttca ggggttcgat gtgtcctacg
    16681 gtgcagtgga gcgcgtaaga gccgccatga acaggcctgg ggttgtccga gtggtcgtgg
    16741 atctcaatgc tccattggga tacaccatat cgcatgagcc cggagtgccg tcccgattgg
    16801 ccattcgctt caacacgctc gtcggggaga tccgactcga ccaagatacg agcattccca
    16861 ggttgcgagt gaagggagac gctccaatag agcacggttc tcctgtggat gacgggggcg
    16921 gttgttggca cattgacatg aaaggcacaa cggcggcagg tgggatcacc gatcttcggg
    16981 acgagatcgg ccccgttaac tgggttaaag catctcagcc tgagccaacc acggcaagac
    17041 tcgaattcgg tttgaagaag ggatactccc caattattcg ttcacctgaa tccaatgcca
    17101 acgagctact gatcgatttc gccatcactg tatcgaaaat ggatgtgttt gaggaacctg
    17161 ggtgcacagt gatccagatt gtgacctcgg ggcccgtgga gttcaagact tttaggctca
    17221 ggagtccgga acgtattggg gttgatctcc cgggtgtgtg ggcaggggga tatggttcgc
    17281 tgccggtgga atccgctggg gtggagaaaa tcagatttgc tcagtttact gctgacgtag
    17341 gcagaattgt gttggacctg cagcccgagg cgactcggtg ggcgcattca gtcgagatct
    17401 cggccgacag gacgacggcc actatcagag tggggagttc acccatttat ggacggactg
    17461 tcatgctgga tccgggccat ggtggatcag atccgggcac agtccatgac ggcctctatg
    17521 agaaggagat caaccttgac attgcgctcc gtgcgagaga cctattggtg aaagccgggg
    17581 ccactgtggt tatgacgcgc gaagatgact ctacagttcc ccttggcgac aggccccgca
    17641 tggccaatga ggtgaagcct gacgcatttt tgtcgatcca ttgtaactcg gtgctcaatg
    17701 tttttccgtg cggaaccgag acctactact ataatatgat tgattacagc caggagctag
    17761 ccacacagat tcactattgc ttgcttaaag agatcatgct ggttgacaga aaggttcgcc
    17821 gcaaggacta ttacgttctc aaggagacac aggtgccagc tgtgctggtg gaagtgggat
    17881 atatgtctac aagtgaggaa aacgagaagc tccgtcagcc tgaattcagg cagaaggctg
    17941 ctcaggggtt ggtaaatggg ctcgctgctt acttcgacag tgacgtctac gctcggtggc
    18001 ggactgaagt tagggccaag tgagggtctg gtggatgacg ttgtaactct ttgcgcggca
    18061 ggagtataat aatcacatgc agtacagaag tgtaggcgtt tttgattcag gagtgggcgg
    18121 tctcaccgtg gccagggcta ttatggaggt catgcccggc caggacatag tctattatgg
    18181 ggatactgcg cggatgccct atggggataa accgcccgag gagatagttc gctatgcgcg
    18241 gcagataatc tcgttcttgg ttgaacgcgg cgtcagcgct gtggcagtag catgtaatac
    18301 gtcgtctgcc atcgctgtgc cagttatcgg gcgcgatttt gatgttcccg ttgtggggat
    18361 gattcaatgt ggagcagctg cagcggcaga agcctctcga tcgggccgca taggcgttat
    18421 cgcgacagca aacaccgtgc ggagcggggc atattcgcgg gcgattgcta accttcgccc
    18481 ctgcgccagt gttttcgagc aggcgtgtcc gatgctcgtg cccatggtcg agcgtggaga
    18541 agtttcaggt ccccgtgtta gagaggttgt tgcaggatac ctagatccgc tgttggaagc
    18601 gggcattgat actatcgtct acggttgcac ccactatcca tttttggaag atgagataag
    18661 ggcagtggcc ggttcatcag ttgccctggt tgatccggcc ataaaggttg cgtcggctgt
    18721 taagacagcc attggggaag atgggcagat ggtgaaaatg cgtggaggat taccctcgtt
    18781 tgtgaccagt ggcgatccgt gtgccttttc ccaactagtc cgctctctgc tcggttggaa
    18841 cgtgcaagaa gtgtggcatg tggatcttga cgcggctgag gccagcgctg gaaaatgcag
    18901 tttgcctggg cgagcagatc cagagtagaa acaccgggag gtatggagtt gggcaggctt
    18961 gaactgacaa tcatcggcaa tcagggccca tattcgggcc ccggcggggc atgctcgggt
    19021 tatcttgtga gatcccaggg agccacggtc ctaatcgact gcggacccgg ggtgatgccc
    19081 aggctccagc agcatatcga tgtttcaagt ctggatgccg ttgttatcag ccatttccac
    19141 cccgatcatt actcggattt tctcacaatg cgatatgcgt ttcagtacat tgagtcaacc
    19201 ggccggatgg gctgtcctgt gaccctggcg cttcctgcag tagatgccga gtggttcttg
    19261 cgggagatca ttggggagga ttataagccg ctcttccgca tcgtccccat agaacatgga
    19321 agccgtctgg agataggcgg cagagtgcgc ggcggggatt gcgggctggt cctggacttt
    19381 cggttgatga gccacgccat ccctagcttt gggacgatcg tgtatgcaag ccaacctgag
    19441 catgggtctc gggcgccgct tgcctacacg tcagacaccg gactctgcga gtctttggag
    19501 gaaatggctg cgagctcgca tacactattg gttgaggcat ctatgcagga aggcgacgca
    19561 cagcggagtt ctgcaggcca tcttgccgct gcggaagccg gcagaattgc ttcagaacat
    19621 ggagtcgccc gagtgcttct cacgcatttg caccccgcca agctgctcga aagatcggtt
    19681 tgcgaggcaa gcgaagccta tggcgggcag gtagagattg ctgccatggg gaagacttat
    19741 gccttaagag agcagcttgc tgaggaaatg gaggatgaga atgaggtttg atggaagaac
    19801 gccggaacag atgcgtccat ttaagataac aaggcattac ataaagcacg cgcccggctc
    19861 ggttttggtg gagatgggcg atacacgggt gctgtgtgcc gcgacatatg aggagaggcc
    19921 gccgatgcat gtgaagggtt cgggtcgcgg ctgggttact gcggagtatg cgatgcttcc
    19981 gcgggcgacg attgaccgaa cgccaaggga gaggaatacc ggaagaattg ccgggcgtac
    20041 gcaggagata cagaggttga tagggagatc cctgagatcc gtggttgata tggcgtcttt
    20101 gggcgaatgc accatttgga ttgactgcga tgtgattcag gcagatggcg gcacgaggac
    20161 ggcttctatc actggagcct atgttgcctt ggtggacctc ctggactcga tggatcagtg
    20221 gaaagggaag ccgctcccag tatccgattt tctgtcagcc ataagtgtcg gcattgtgga
    20281 tggccagttg atgctcgacc tgtgctatgt tgaggactcg catgcttcgg tcgacatgaa
    20341 cgtagtaatg accgagggag gaaagtttgt agaagttcag gggacagccg aaggggagcc
    20401 cttcgcacgc gagtcgctcg actcaatgct ggaattggct gagagtggaa tccgcgaact
    20461 ctgtgcgcat caacgccgtg cactgggcga gctagccgag agagtcggca tcaatggtga
    20521 gaacaacaga tgaaacggct tgtggtggcc acgtcaaatc gtggaaagtt tgaggagatt
    20581 gtacacatac tatctggcct tgacatctct gtcgagagtc tgtctgatta cccaggtatg
    20641 gtacaggcgg aggaaacagg ggagacgttc cgtgagaatg cgctggagaa ggcgattgct
    20701 gcagcgaggc atactcatca ggtagctttg gctgacgact ctgggcttgt tgtggatgct
    20761 ttggggggtg cccccggagt gatgtcggcg agatatgcag gcgcaggtgc ttccgatagt
    20821 gacaaataca ggaagctgct tttggcaatg gaaggcgtgc ctgagggtga gcgtacggcg
    20881 agattcgtat gcgttgtggc gattgcagct ccagatggcc gagcctggac agcagaaggt
    20941 attgtggaag gcatcataac cagagttccc cgggggaatg gcggattcgg ttatgatcct
    21001 gtgtttctcg tcccggaact ggggctgacc ttcgctgaaa tcgacccgga aactaagaat
    21061 cgaatatccc atcgtgcacg ggcccttgcc aggtcaatcc ctatggtaca agaggcattg
    21121 ggtatttgac gcgcgcctgg gcgtatggta gactcgtatt gttcggggcg tagctcagtt
    21181 tggtagagcg catggtttgg gaccatgatg tcgcaggttc aaatcctgtc gccccgacca
    21241 ttcgttgaaa agtgataatg ggtccataaa ggtgatgacg gagagtagta ggttgggagt
    21301 tgcccagaca gggagggtgt gccgtgactg caagcgcccc gggtaaggcc ccatcgcgaa
    21361 ttcgctcccg aaccgcacgc tgaaagacgc caaccatgag aaggttcatg ttcttgtccc
    21421 cagtggcgtt ctggtaggtg tagacggggg cctcccgtta caggggcagg gcatgaagtg
    21481 tgcctgatga agaggcctaa cggcgaagat gggtggtacc acgtagtcca cgacttgcgt
    21541 cccatgcgca cagtcgtggg cttttttcta tatgcagaac ttttcggagg cgggctgagg
    21601 gtgacacata agcgggcgaa gaaagtgagg ctcgatgcct tgctggtcga gcggggcctc
    21661 gtagaggatt tgaagagcgc aagggcatgg ataatgtcgg gagacgttat cgttgatgac
    21721 atccgggaag acaagcctgg aacgttgata aggcccgagg ctaccataag gatgaggggc
    21781 aggaacatac ctttcgcaag caggggcggg ctcaagctgt cagccgctct cgacgccttt
    21841 agagttgatg tcagcggaag gataatcttg gacgcaggcg cttccactgg cggttttaca
    21901 gattgccttc tttctagggg cgctgcgcgc gtttacgctg tcgatgtagg ttacggtcag
    21961 ctcacgggaa agcttcgcca ggatccgcgc gtcgtcaata tggagaggac caatataggg
    22021 gatattgtgc ttgggcagct cgacccggta ccagacatgg gggttatcga tctttcatat
    22081 ctatcgctgt cggttgcaat tccgactgtg gccagactac ttgcgccctc ggtgcctggg
    22141 gccgaaccgg agatcattgc tctaataaag cctctgttcg aggtagctgc ttcaggacct
    22201 gatctcacac gggatcaata cagagctgct gtagagaggg gattgcgtgc tggagagggg
    22261 atcggctggt ttgcccgtgg ggtcatggcg tcgcctgtac tgggatcagg aggaacactg
    22321 gagttcctcg cacattacag ccttggcaaa gtccacggcg acttggacga gaagatagat
    22381 gaagctgtgt gttcaggcat gcagcttgtg gaaaatgccg gtagcccgca tagccgggcg
    22441 ggagggatgg aggagagtga tgaaggaaat gaatgacaaa gtggaagcag tggttgaaag
    22501 agcgctgtct gcgatagaaa atgcgcacac gactgctgcc ttagaggagg cgcgggtcag
    22561 gctcctcggg cgtaagggcg aactgactac tctcttgcgc cagatgaaat ctgttgatcc
    22621 tgaagacagg ccgaagttcg gagctttggt caattctgcg cgcgaaacaa tagagaagga
    22681 actggatgag agggcccgcg tgcttgtggc tgcggaaaag gcggcaagac ttgagctgga
    22741 gagaatagat gtgacgatgc cgggcagggc gccccgcatc gggaagattc atcctcttac
    22801 ccgaggaata atggatatta ccgagatctt cgttgaaatg gggttttcgg ttgttgaagg
    22861 gcctgaggtt gagactgact actacaactt tgaggcattg aacaccccca aatatcatcc
    22921 agcccgcgat gtacaggaca cgttctacgt caatgataca gtattgctta gaactcaaac
    22981 ctcgcctatg caggttaggg agatggaaaa acgcgagcct ccggtgaaag tggttgttcc
    23041 cgggagagta tacaggcggg atcaggtgga tgcaacacac tctcctgttt ttcaccagat
    23101 agagggcctt ctagtcgatc atgacataac atttacggat ctcaaaggca cgcttgccat
    23161 gtttgcacgc aagttttacg gcgaagaaag gaaggtcagg ttcaggccgc attatttccc
    23221 atttacagag ccttcagctg aaatggatat ctcatgcgct atttgcgggg gcgccggctg
    23281 cagggtatgc aaacaggaag gctggattga agtgctcggc tgcggtctgg tccaccctaa
    23341 cgtgcttcgc gcggtagggt atgatccgga tgagcttacc ggatatgcct tcggcatggg
    23401 agtggagaga ctcgtgatgc taaaacatgg catagacgat ctgaggctgt tgtacgagaa
    23461 tgacctgagg ttcttgtcgc agttttgaac tggttgaaga ggggtgagaa taagtgcttg
    23521 tgccacttaa atggctttcc gattatgtcg atatagatct ttgtatcgat gagcttgcag
    23581 ataggctgac aatgagcggg accaaagtgg agactgtaac agatatcggg gataggctca
    23641 atcgtattgt tgcaggaaaa atagtctctg taagcccgca tgagagtgtc ggccgtcttc
    23701 gcgtggctac tgtagacttg ggcccgcgcc aggcgcggag aactctggta acggccgctg
    23761 cgaatattgg caggggtgac attgttccag tggtcttggc gggcgggacg atcgccgatg
    23821 gaacggagat aggtgaggta gatttttccg gagttaggtc ggagggaatg ctctgttcag
    23881 ccaaggaact tgggatttct tctgatggat caggcattct gcgcttgccc gacgatatca
    23941 aacccgggga cgacgtagtc tctgctctgg gattggctga cactgtgata gaactggaga
    24001 taacgccgaa caggcctgac tgcctctgca tgatcgggat cgcgcgcgag gttgctgcta
    24061 ttacaggtgc aaccctcaag atgcccaaca ccgcactaaa agaaggcagt gagcgggcag
    24121 aagaacttat cagcgtgaag attgatgccc atgacctgtg ctctcggtac ggagcccggg
    24181 taatacagaa ggtttctata ggccagtctc caatctggat gcaggttcgg ctgtcagctg
    24241 caggcgtgag accgatcaac aacgtggtgg atgtgacgaa ctacgtcatg ttagaggtga
    24301 atcaacctct ccatgcgttc gattacgatc gcatgtcgga gcatgccgtt atagtcaggc
    24361 gcgctcgttc cggcgaggtc attgttacac tcgatggcga agaacgcagg cttgacccgg
    24421 agatgctcgt tattgcagat ccaggctcag ccctggctgt ggcgggcgtg atgggcgggc
    24481 tttccacgga gattaccgag gatacatgca ctgtcttgtt ggaatcggct acgtttgctc
    24541 gccagagtgt ctggaagact tctcgaagtc tgaagcttcg aactgaggca tcatcacgat
    24601 acgaaaaggg gttggacccg gagacggttc ctttggctct ggatcgcgct ggccatctgc
    24661 tcgaggcgat tggggcaggt gttgctgcat ctggaattgt ggacgcctat ccatcaacgg
    24721 cagctgttag ggttataagt acggatgcgg ccaggatatc aaggcggctt ggcgtggatg
    24781 tgagctccga tgatgtcgcg gattccctgg ggcgcttggg tttctctgtg cgtgtcgaga
    24841 gcgtggcgaa caaggccaag tctgagggcg gctcgcttgt tgttgaagtt ccaactttca
    24901 gaggggacgt tcaggaagag atagatctcg tggaagaggt cgcccgaatg tggggctatg
    24961 ataggattcc gacgacagtt cctgcaggag cccacgtcgg aggatacaca tacgagcaag
    25021 aaaaggccat gcaggcgaag acgatcctga cgggcgcagg cgcgagcgag gcgctcacga
    25081 tcccattcat gtctgtggcc gatcttgaca tgctcagggt tgggcagggc gatcctttgc
    25141 gcgatgctat tcgcattgac aaccccatgg tcgaagagca atcgtacatg aggactacga
    25201 tgttgccgtc aatgcttgtg gctattcgaa ccaacatcaa caggggtaat aggggagtat
    25261 gcctattcga gctaggcaag acttactttc cgtcccaaag cctcaaacgt ggagaagagc
    25321 ttcgtagagg cgagaatcca gccgtcgaga gacaggttct tgtatgcggc atggctggtg
    25381 catatacccc gcgtatctgg catactcgtg aacgtgacta cgatttctac gatgcaaagg
    25441 gaatgctgga ggcgcttctt gaagggatgg gtgtggacgg agcaacctat gttcagacgg
    25501 tgcgcccttc attccatcct gggcggacgg cttccatagt atgggaagat cgggaacttg
    25561 gcgttatcgg agaggtccat cccgatgttt cagaggcgta cggcgttgcc ggacgcgtct
    25621 acatgttcga gctggacttt gcaagaatgt tggaagccgt agatgaagcg aagtcatacg
    25681 agccattgcc gaggtatccg gcaatagagc gtgacgtggc aatggtgttg cctcgcgcag
    25741 tctccattgc agatgtggct gagcttatcg aaaagaccgg gggggagctt gttcgctctg
    25801 tcacgctgtt cgatgtttac gagggcttgc ctgtgcctga gggaaagcgg agcatcgcct
    25861 ttaccgtaac ctaccgcgcc caggaccgca ccctcactga tgaagaagtc aaccgcctgc
    25921 acgaggaggt caggaaggct ttgacggacg agatgggcgc agtccttaga taaaacatgt
    25981 gcgtgcgaga aaatgcagga atctgcaata ttgttggcaa acaatctacg tgataggacc
    26041 tgattgccga ggggtgtcag gagccgcatc atggatgcag gagggtgatg ctgatgttga
    26101 cggagcaagg tcccgatgga ttagatcagg aaggcgtgcg ggtgtccgca gtcatcttgg
    26161 gtgaggaata cgtgatacgc ggggataccg aggcagaaca gattgaatcc ttggctaagg
    26221 aagttgactt gcgaatgaaa gcgatagcgg aggccaatcc gaggttgtcg tctcagcaag
    26281 ttgctgtgct ctctgccctc aatctcatgg gagaactcgt tgtgtcaaag cgtcgctact
    26341 ctgatctcct tgacctgttg gagcaggttt agatacggag gtgaggcttt ttgactgtct
    26401 cgtctcttga ttggcttagc atagtcctca tagtgatact cgttgcagac ttcgttgtgg
    26461 gctatcgtcg cggatttgtg cggcaactat tcgatattgc gggcataata atcgcaatcc
    26521 tcgttgcggc caaccgctgg cagggcgctt ccggtcttct gcagaaatgg tttccaaaac
    26581 tgcggcctcc cggcgctcac atggtaggct ttttggcatt gctcataatc actttagtag
    26641 tggtcgattt gatcgggcag gcattgtcat cggccgtgag cgcctcggga gtgtcggcgc
    26701 ttaactgctt tggcggcgct gcgctgagaa tcctcaggtc ctgtcttatc atcagcatcg
    26761 tgctggtggc tctggtctcg ttgaggatac ccagccttac caggactgtg gagtcttctc
    26821 cggttgccat gaagataatc aaggttgcgc cttccatatg ggacgggttt gagaagttca
    26881 tgccggagca atttcgtcct tcgggacctg ggggggcgaa aggtgaacca gctaaacctg
    26941 gcggtggcgg cttcatttag cgagatcgca gatcttctcg aaatcatggg ggataatccg
    27001 ttcaaggtaa gggcgtacag gcgagccgcc gaggctatcg ctggattgga cgagccagcc
    27061 tccgagatcc ttgcgcgcga tcctgggagc atacccgggg ttggggatgc tcttcgggca
    27121 aaggtggaag agatggagtc taccggcaga attgagtatc tcgagaagct caggctcgaa
    27181 gtgcccgccg ctatattggc gttgatagag gtgcctggta ttggtccgaa gactgcgtgg
    27241 aagctctatg agtccttggg agtggaggac gaggccagtc tgctggcggc tgcgcgggcg
    27301 gggaagattc gggatttgcc agggtttggc gagaagacgg aggataagat aataagggca
    27361 atagagaaca tgaaatctac tgaggaacga agacctctat acaaggtatt gccgattgcc
    27421 catgatttcg aagccatgct tggcgcggtc gaaggagttg agcgagttgg catcggcggc
    27481 agcttgcggc gaaggcgcga taccgtaggt gacatcgacc ttatcgcgtc gactcgagcc
    27541 ggagatctcg ttatggaggc cttctccgcc agcgacatgc tgagggagat aaccgcgatg
    27601 gggcctttca aagctgaggc gattgcggtt tctggtatta gatgtgagat acgggttgtt
    27661 gcccctgagt catactggac aggctttcat cacataacag gatcaagagc gcatcacgta
    27721 aagcttagaa gaatcgccgg cagacatggc ctaaagataa gcgaatatgg agtgcgccgc
    27781 gatgacacgg gggagatgat ccccgtgggc agtgaggagg agctttatgc tatcttgggc
    27841 atggactaca tccctccgga gcttcgggag gattggggtg agatagaggc tgcactcgag
    27901 ggaaggcttc cggatctcat tgacgaaggc gcgattcgcg gagatcttca catccatacc
    27961 aactggagcg acggcgtagc ttcgatagag gaaatggcaa gagctgctcg ggacataggg
    28021 catgagtaca ttgcaatcac tgaccattcc cgatctttgg gcatcgcccg cggcctatca
    28081 gaggataggc tggcagaaca cattgaggcg gtgcgcagag ttgatgccaa gattgacggg
    28141 atcaggattc tttctggagc agaagtcgac atactaaagg aagggcggct cgactattca
    28201 gatgagcttc tttccaagtt agatgtcgtc atcgcatctg ttcacagcgg atttcagcag
    28261 gatcgcgata cgatgacgag gagaattatc gaggccatgc gcaatcccaa tgtcgacata
    28321 ctggcgcacc caacagggcg gcttcttgga cgcaggccag gttatgaggt ggacatagaa
    28381 gccgtgatac gagaagctgc agctaccggg actgcgttgg agatcaactc atatccagac
    28441 cggctcgatc tccgagacga gcatgcccgc atggcgcgag accgaggagt gatgatttcc
    28501 atcaacaccg actcgcattc tccggaagag ctggagaaca tcagatacgg cgtttgggta
    28561 ggcagacgcg cctggcttga acgccaggac gtgctcaatg ccatgacgtg cgaggaactt
    28621 cttgtatggc ttggttcaca caagcggcac atgtttccgg tctgagcgtt ttgctgggaa
    28681 gagatgtccg gaatacctcg aattcgcgta tgggcatact aatgtatgcg ccgtgcgcga
    28741 gtcttttttt gtggaggcta agatgtttca cgcgatagtc cgatcactta cggtctactt
    28801 cttcgttctc gcatccatgc gtctgatggg gaaacgcgag atagggaata tatccccttt
    28861 cgatcttgta gttgccatca tgatcgcgga attgggtgct attccacttc agagtcatga
    28921 tattcccctt ggccacggtc ttgttcctat agctatactt gctgctgtgg aagtgttggt
    28981 ggcccttgct tgcctcaagt ttccgggaat ccggagatta gtgattggcg agcctactgt
    29041 gataattgag gatggaaaag tgatgggacg aaacatgcag aaactccgat acaacatcaa
    29101 cgacctccta agccaactca gggacaagga cgtattcaac gtggcagacg tggagttcgc
    29161 tcttctggag ccttctggta ggctcagcgt gctcttgaag tcgcagcgga gaccggtgac
    29221 gcccgaagac ctgtcaattg agactcatta cgaagggcca tcttacccat tggtagctga
    29281 cgggaaaata cagtatcaat acctgaagat gctcgatctg acagtgaaag acctcacgga
    29341 tatgctgaag gcgaaggaca tacaaagtgt tgaagatgtc ttctttgcct ctctcgacag
    29401 cggaggcgaa ctgtacgtga ctatgcagga cgaagtttgc ctcgacgagc ttgaacgggg
    29461 agaataggaa gtgtgagggg gtagagctat ggagctgtct cgcgaatttc gggagaggtt
    29521 tgtccggagc ttgcagggac gagactacat cctttacggc ggcgttttgc aattagctaa
    29581 ggagcgcggg ctgaggcgca taagcacgcg gataattcag atccccagca aggataattc
    29641 gatgtatgcc gtggtggaag cggaagtgga aactgaggat ggagtctttt ccgaggtggg
    29701 cgatgcttcg cccgaaagcg ttgcccgatc cattcagccg caccttctca ggatggcagc
    29761 gaccagggct aaggcgcggg cgatgaggga cgccgtgggc atagacatgg ttgccctcga
    29821 ggaactgggg gatgctctgc cagataggga tgttatggat gaggaggttg atttgcgaag
    29881 tccggaagat ctcgttatta ggttcggaaa atactcaggg aagactctgg gagagatata
    29941 cggagtcgat cgcggatacg tggaatggct gagtcagaac gcgagggatg aagccataag
    30001 agacgcgtct agggagttga tgttgcccag cacacctgag atgtgagtct gctataattg
    30061 aacagagcgc ggcgagcggt gggtccggaa tgccccggac ccatttcggg tatgtcgtcc
    30121 gtgccaatgc gacatgtggg ggagttctca tggacgaccg ttcattgcgt gttctcgagt
    30181 attacaaagt tcgcgacatg ttggagcagt gcgcttcgtg cgagcttggt agggagcgca
    30241 taaggcagct ggctccgatt acaaatcttg ccatgattga ggaagcccag cgggggacta
    30301 gcgaggcgag atcgctgctt gacatgggga aacagatccc atttggcgga gtgcgcgatg
    30361 tgcgttctag tgtggagagg gccgatagag gcgctactct ctcctgtgat gagctccttg
    30421 atatcctgtc aacgctgtcg acaagcagaa gcctgaagaa gatcttgggc agccttggtc
    30481 cggagtaccc tgtgcttgct gatgcggctg ctgacattgg tacattcggc gatttggagc
    30541 aggcaatctc gcgggcgata tctgaatccg gcgaggtgat ggattcggcg agctctgctc
    30601 tgagaagcat tagatcgagg atacgctcgt taaatgcaaa agtgcgtgat aatttagatt
    30661 cgatggtaag atctcctcaa tggtcgcgcc tccttcagga tccaattgtg agtatcagaa
    30721 atgggcgatt tgttgttcca gttcgcgccg agatgaggtc gcaggttccg ggcatagtcc
    30781 atgaccaatc ggcttctggg gctacagtat ttgtcgaacc catgccagtc gttgagctca
    30841 acaacgagct gaggcaggcc atatcccagg aagaagccga ggttgaacgg gtgctctccg
    30901 agctttctgc gaaagtgggg tctcagtgcg aacaactgag gctgactttg gatgctatcg
    30961 gcaatatcga tttcatcatg gccaaaggga gactttcgca gcgcatgggg gcctcagtgc
    31021 ctcaactgaa cgacatgggc catatcaacc tgatagccgc gcggcatcca ttactcaaag
    31081 gcgatgtcgt gcccatagac gcttacctcg gacagacatt cacgacattg gtaataacgg
    31141 gtcctaacac cggaggcaag acggttacgt tgaaaacaat tgggttgatc accctcatgg
    31201 cccagtcagg gttgcatatc cccgcagagc ccggcagcga tgtagctgtg tttagcaagg
    31261 tttttgcgga catcggagat gagcaaagca tcgagcagag cctctcaacg ttctcatcgc
    31321 acatgacaca cattgttgcc atcatgagca tggttgacca cgattctcta gttctccttg
    31381 atgaactagg cgctggaacg gacccacagg aaggtgcggc gttggccatg gcgatccttg
    31441 agactcttca cttgactggg gcgaggactg ttgctacgac ccactacagc gagctcaaga
    31501 tctttgcgca tacaactgag ggtttgtcca atgcgagcgt ggagtttgac gtggagacgt
    31561 tgtgtccgac atacaggttg tcgataggag ttcccggggc aagcaatgca ttcgccatcg
    31621 ctgaacggtt gggtttgccc gaggaagttc tcgatcgggc caggagtctg gtgggaactg
    31681 ggggccagcg catggagctg atgataacca atctcaagga ggaatgcgac cgggcggaga
    31741 cggcgaggga agatgcggag aagctcaggt cgcggtatct ccgtttgaag gacgaatacg
    31801 aggaacaaac gcgaagactg cggtctgaaa gatcggagat catgagagcg gctcgggcgg
    31861 aagcagagtc tattattcag gaggctcgca aggaatcgga aaggattatt gggcggctcc
    31921 gcgcaatcca ggcgcagcag gaacttgtgg caaagcgtct tgtcgaggaa gaggttatca
    31981 cggaggattc gctccctagg tcggctgtgt gtggagaagc gtccctacct gatgaggcca
    32041 ttcgtgcggc ccgtcgcgac cttgaggctc tcagggaaat aactcgcgag ctgacagctg
    32101 tagctccgga atccgatgga gaagtgacca ccattgccga tgtccacgac gacggttgga
    32161 gcagtaggat agaacagggc gacgatgtag aggtggtggc ccttggccag aagggtgtgg
    32221 tcttggacca acttgggccc gatgaataca tgattgggat agggcccatg cgcgtcaacg
    32281 tgaaccgtgg ttccattcgg aagcttcgct cttcgcaagc ggaagaacct gatcgcggat
    32341 atggaagacg tggaacgatg cgggagccgg gatcggtaag ttttgaaacc caaaaagctg
    32401 ccaccatagg ggcagagctc gatcttaggg gaatgcgtgt ggaagaggct ctctttgccg
    32461 tcgacaagta cttggacgat gcagttcttg ccggcattca gaaggcgcgc attatacatg
    32521 gaaaaggaac gggggcgctt cgcgacgccg tcaccgagca acttggtagc gatccgaggg
    32581 tagcgtcctt caggttcgga ttggcgggtg aggggggaga cggcgttacg gtggttagct
    32641 tcgggacgcc cggagc
//
LOCUS       DAOSYL010000020        32530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1126_length_32530_cov_7.397968, whole genome shotgun sequence.
ACCESSION   DAOSYL010000020 DAOSYL010000000
VERSION     DAOSYL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 32530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32530
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1126_length_32530_cov_7.397968"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(480..920)
                     /locus_tag="PLU15_04990"
     CDS             complement(480..920)
                     /locus_tag="PLU15_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOO30309.1"
                     /translation="MLWQRRQMPGADMEIASETDLGAKSPKERGTRSGTGRSAQKDTH
                     LTGGVSVSPMPITAGEEVTVKYNGLLAQSGADSVYLHAGFGAAGSWHDVVDIAMLQEK
                     PGVWKATLPLVPEETSRLNFCFKDRAENWDNNYGLNWSFDIHNG"
     gene            complement(1326..2636)
                     /locus_tag="PLU15_04995"
     CDS             complement(1326..2636)
                     /locus_tag="PLU15_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO30310.1"
                     /translation="MGALLIEDVTVITMDKYRRIIPKGAVLAESGKITFVGSVDEVPN
                     DLPANLGRISGRGRIAMPGFVNTHTHAAMTLFRGYADDLPLQEWLETKIFPIEARLSA
                     EDVYWGTLLACMEMIRSGTTTFADMYFFMDEAAKAVEESGIRACLSIGMGSLDGKGDE
                     KLAKSVEFCQRWQGAAGGRITTMLGPHAPYTCSRSFLSKVAQSAVDLGVGVHIHISET
                     EREVEDCKRQFRMSPVGFVEASGVFQAPVLAAHCVAVSDQDIEILAEHDVRVAHNPGS
                     NMKLASGVSPVPKMMRAGITVGLGTDGAASNNNLDMIEEMRLAALLHKVSLKESTAMP
                     ARTCLEMATIDGAACLGLEDSVGSLEVGKRADIILIDFDAPHLTPTSTVDLASHIVYS
                     SCGADVSTVVIDGVVVMDDRKIIGIDEEKVMSEVDRRALRLVIG"
     gene            complement(2739..4004)
                     /locus_tag="PLU15_05000"
     CDS             complement(2739..4004)
                     /locus_tag="PLU15_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOO30311.1"
                     /translation="MGDSVIRDAGLAPEGWIKIEWARAHMPVVEAIEKEFARDMSLEG
                     LNIVVCCHLEAKTARLAMALAAGGARVAVTGSNPLSTQDDVAAALAQEDGISVYAWHG
                     ATGEEYLQFLDMALDLRPDVLMDDGGDLVGRVHTARRELIPQILGVCEETTTGVHRLC
                     SMEAAGELAFPAVAVNDARMKRLFDNRYGTGQSAWDAIMRTTNLSIAGRCVVVAGYGW
                     CGKGLAMRARGLGAEVIVTEVDPVAACEASMDGFRVMPMERAAGEGQIFVTATGCRDV
                     IRREHLERMRDGAILANAGHFDVEIPKPDLASISTGSPTQVRPNIEEYRMPDGRRLYL
                     LAEGRLVNLAAGDGHPVEVMDMSFGVQALSILHIAEHGKELEPKLYSVPVEVDRRVAV
                     LALEAAGICIDSLSEGQRQYMASWQTGTA"
     gene            complement(4028..4840)
                     /gene="mtnP"
                     /locus_tag="PLU15_05005"
     CDS             complement(4028..4840)
                     /gene="mtnP"
                     /locus_tag="PLU15_05005"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOO30312.1"
                     /translation="MRIAIIGGTGVYDPRILSNVREETIDTPYGDAGLRIGEYRGEEI
                     AFLARHGFGHTVPPHMINYKANIAALKMLGVERVIATSAVGSLNPDMPPGMFVFFDQF
                     IDFAKSEVRTFFDGGEHGVVHLDYGVPYCPEIRGVLYEKARKLGIEAINGGCYVCTDG
                     PRFETPAEIKMFETLGGDLVGMTSVPETVLAREAQLCYSSIAMITNWAAGISPTPLTH
                     QEVVDIMEANGEKLRSLIMATIEDLPQERGCGCAAAGVDIPWLDEYKKTHWR"
     gene            complement(4911..6101)
                     /locus_tag="PLU15_05010"
     CDS             complement(4911..6101)
                     /locus_tag="PLU15_05010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO30313.1"
                     /translation="MKLSERARAIKPSPTFAVEAKAREMRARGIDVIGFGAGEPDFDT
                     PEHIRDAGIDAINSGFTRYTPTAGIPELKEAIVAKLRRDNGLDYEPSQIIVSCGAKHS
                     LYNAFQVLCGPGDEVIIPAPYWVSYPDQVRMSGARPVIVDTSSTDFKITPRVLADAIT
                     PRTRAIILNSPSNPTGVMYSQDELSAIAEIVVENDLFVVSDEIYERLLYRGAEFVSIA
                     ALGEEIKARTLVVNGVSKTYAMTGWRIGYAAGDSELISAMNKIQSHSTSNPCSISQRA
                     ALAAITGPDEPVQYMVEQFRVRGEYMHERLSGMPGIECVEPVGAFYCFPKVSSYYGKS
                     LAGEVVKDSVSFADAILEKAHVAVVAGAGFGTEDYVRMSYATSLEKIKEGLDRLEQAL
                     SEMK"
     gene            complement(6234..7544)
                     /locus_tag="PLU15_05015"
     CDS             complement(6234..7544)
                     /locus_tag="PLU15_05015"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOO30314.1"
                     /translation="MSSVTEIAEALEKTLSQARGWRFVAKSTESVDIGVDDSKLGGAY
                     SPPRSSSEISGNVFIVWEDGRCSRGRVDSKILDRLPRELDLWRSLAYRDDDAAYIWDL
                     ESPADVKLFDPKVAGVVQGDPTPAFEGIGRIVEAALDSGSMVVDARVCCSAGETVVAT
                     SNGLWAKYSETGAWCYWYLDKTLGDSRYGRTTGTWQALDESVEWTARIVDAARIPASS
                     NSGTMRVIMSPSVVDSFIGHYIAFNLSGENVAEGRSRYEVRQFRGGEPALRDDMQVAL
                     DTAVPMGPGSYPCTVEGVASGRADLIKDGRLVTPVLNLKYARRCNMQPTPLPSRGLSP
                     GYAGMLLRTENMGAAESLLEECQDALLVCSVLGMHTQDPVSGNFSLAAPEAIVVKGGE
                     MLGSVKAVISGNFFDALNSRQTRLSLDDQHPNPWMECLCHVEVG"
     gene            complement(7546..9036)
                     /locus_tag="PLU15_05020"
     CDS             complement(7546..9036)
                     /locus_tag="PLU15_05020"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO30315.1"
                     /translation="MTLRVGAYTEACNRAMETARRLGVYAIVRIQDKTERAVGVMNGQ
                     TDLLSIEELSGVGVQIFTFEGYSGFASSDMITLESLEHLVESATNLAKASAKYGAQPS
                     REINQARPLIRDIDVPVPMGLDSISHDEEERLVREVCETAMKIDPRLAVRSFYRIANE
                     QWRVVRSDGTDTMFSMPRSAAMNMITARDGGRAASANASLAGADASIIASSDGRSRLD
                     KRTRKAALTALALLDAPRVAAGPYRVVIDYALAKGLAHEAFGHAAECDGMETSILGLN
                     GKFRKGHRVASDIVNIVDGPVEGDYAYQPISANGIFRATVDIVRRGQLCCGLADIFSA
                     ERAGVPVTGAGRAESFRHVPVPRMTNIRITVENPLPIHKEFEDVEPEDVRDVLARAGL
                     LLDDEPVLYLSGYKGGQVNPAKGDFVFNCSAIYELTHDDIRICQPAIFSGKVLSALGS
                     IIAGIGEPMIDAMGTCGKSGQSVPSSGGGNMFLVIDGNDEITIGGV"
     gene            complement(9033..10997)
                     /locus_tag="PLU15_05025"
     CDS             complement(9033..10997)
                     /locus_tag="PLU15_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOO30316.1"
                     /translation="MSVLEIDSLKKSFGASPIFEDVCLSVAEGDHIGLVGRNGSGKST
                     LLKIIAGIEDYDSGSVALASGCEAGYLAQGKEYTSGNSLYEEMKRAFAKTFEIGAEMR
                     ELESRMSDPEIIASERELDRVMRRYSRLTADYELLGGYDYDVRIKSTLFGLGFVEEDL
                     ERIIDTLSGGQKVRVALARMLVSAPNLLLLDEPTNHLDVNACEWLEDYLAAFNGAFIV
                     ISHDRYFLDATVDRIWDIDDLKVVAYPGNYTFFAAEKELARQRQAQEYQRQQETIQKL
                     EAYIRRYKAGNRSTMAQSREKMLARIERIQKPREDSQMKLSFGKAARSGRLAVSVSGV
                     SKSYGDKTLFEAVDLAVERGERLGVVGPNGSGKTTFLRIIADDLAPDSGSVDFGENVT
                     LGIFWQDLAGLDDTLTVLDELYRARDWTLGEARSYAARFLFRGEDVFKPVKALSGGER
                     NRLILAKLILNGPNLLLLDEPTNHLDIESRHALEEAIAEFDGTVICASHDRYFLDQTA
                     TSILEIADGRWKLYNGNYSYYREKKTRDEAEAARVAAENAEAALEARLKASGVAPMRA
                     KARAGASGRDGVGHAQAVVEAIEAEIELKETQVGKVEAELASEELYVDGERAREAVLI
                     HRQLTEELEALYEAWEKALDEVDVTGGCED"
     gene            complement(10998..11786)
                     /gene="folE2"
                     /locus_tag="PLU15_05030"
     CDS             complement(10998..11786)
                     /gene="folE2"
                     /locus_tag="PLU15_05030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOO30317.1"
                     /translation="MRDVQNFPDLRGINIQKVGVKDVHLPLQIAAREGGCQSVLGIVS
                     LSVALPMQFKGTHMSRFMEVLLPWSRKPISNVEILAILKETCEALSAQRADISIRFKY
                     FVPRIAPVSGKESILDYNCSFEGTLDGKRHSFAMGVEVPVTSCCPCSKEISEYGAHNQ
                     RAMIRARVKFSSAGFLWLEDLIELLESEGSCEIYPLLKREDEKYVTEAAYDNPKFVED
                     ILRDVVIRLRNEPRVRWFEVECESMESIHNHSAFACHVEDKRMV"
     assembly_gap    12196..12295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12501..15002
                     /locus_tag="PLU15_05035"
     CDS             12501..15002
                     /locus_tag="PLU15_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO30318.1"
                     /translation="MKAMLLGAGEGTRLRPVTATRPKPMIPIANRPIMEHILLLLKSH
                     GVRDVYSNLYYLADQIESYFGDGSALGLSLKFKVEEKLPGTAGGVKNLEEHFDETFCV
                     ISGDLLTDFDLTRAHEFHRSRGAIATILLTHIDNPLEYGVVITDKDGRIQRFLEKPGW
                     AEVFSDTINTGIYILEPAVFDFIPPGVDFDFSRDLFPLLLREGQPLFGYVADGYWCDV
                     GNIDMYLKAQVDALSGKVKLNIPGEQITPGIWVGRSAKISSRADLKGPMVLGENCEIA
                     PGARLREFTVIGDNVIVAPNSFVVRSTVFSNSYIGENSIVSGSIICKNVVLKDSVRVG
                     EGAVISDDSILNRSVTVKPGIRVWPGKSIDESVVLSSSVVWEQRLRRELFHSGKISGL
                     VNFEFAPEFALRLGNAIGGTMKKGESVIVSRDPSRTARMMKRALAAGLSSSGVTVCDL
                     QGVPAPILSHMVKRGDFAYAIHMQTSDRNYELVDINVFDSEGLALSREAERKLETLLA
                     REDPRRTSAREVGGIGYYPRAIDIYTEDCLSRIDVETTKANRMKLVVDCGYGFGGSIL
                     PSMLVRMGVEVTSLNAIEQETKMPKSAEQFVESLDELALIVKTVDADMGIAIDPQGTK
                     MWVTDDTGRVLTDMELAYLFSLLTVRSRDSRNVAMPSYMPSVFTQSLTANGATVYRAR
                     ANARSLADLARTSKSSISSDLVGGFIFPNLHNGFDAIFGAAKFTELVARDAAPLSQLA
                     QLVPEHHLLFDTVPCPWELKGQAMRELLSKLDDPTVETYDGYRRSFGDAWYMVLPDPV
                     HPSFMIYAEGATGAEAERMLSAAREEIVAEMRKNV"
     gene            15405..16298
                     /gene="dpsA"
                     /locus_tag="PLU15_05040"
     CDS             15405..16298
                     /gene="dpsA"
                     /locus_tag="PLU15_05040"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HOO30319.1"
                     /translation="MANLHGRHICLLGGDSRELKLVCALIDRGARVSCYGVPKDGLHP
                     EAIAAANAKEACRGANAIVLPLSGTDSNGNVRVTSSPICITRELLFVLSPNAILFSGI
                     VPRFLAAECQSLGIRTVETTANDELAILNSIPSAEGAILMAMQATPFTIHNSNCLVLG
                     LGRTGMTLARMLHGLGAHVWAAARKAEDRARAFEMGLSPLSFADLPSCLPQIDIAFNT
                     VPALVLDRSLVYSMKRTAAIIDLASSPGGVDFIAASERGIHAELAPGLPGKVAPVTAG
                     QIISKVLPEIIAAEFAKNKPD"
     gene            16374..17672
                     /locus_tag="PLU15_05045"
     CDS             16374..17672
                     /locus_tag="PLU15_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO30320.1"
                     /translation="MTVAVAGGGWAGCAAAVAAAQAGADVVLIERTNLLLGTGLVGGI
                     MNNNGRFTAARELASMGGGYLLDIIDQATLHRRINFPGHLHASLYDVAKIESRVREGL
                     ESLGIHVMLESRVTDVEMNGRRIAHLVLADGSRIGADAFVDATGTAGPQGNCARVNGN
                     CVMCILRCPAFGPRVSIAGKAGANEISGSLFEAMSGSCKLEKKSLSVWLVRSLEENGV
                     LVVPVPRYIARLDKTASKACQQYSLPEFYDNLVVLDTGHAKLMAPFFPLDCLRRIPGF
                     EHAVFHDPYAGGRGNSIRYNIITPHDQAMKVEGIDNLFCAGEKVGLLVGHTEALCTGS
                     LAGNNAVRSCVGQESLVIPQSLAIGDLIAFIEQSLRTSTGFLNKYTFAGGVYFRRMYD
                     SGLYTTDVRRINQRVKAAGMDGVFNRKLVQDKHRTGWLPT"
     gene            complement(17915..18778)
                     /locus_tag="PLU15_05050"
     CDS             complement(17915..18778)
                     /locus_tag="PLU15_05050"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOO30321.1"
                     /translation="MTTLRRLTACNNRFGRSMNTSQVRPWAAIVCVFAMILACSIPVC
                     GQPKVPAKPVPPRLMNDLAGMLTSSEVDDIEERLVALNRANGVQFAVVIVPDMGGYSI
                     EEYSVELFQKWGIGEAKEDNGLLLLIAEDEGRLKFETGYGLEGDLPDSFLGRVIDENL
                     VPHFVEGDPAAGIVEAINTIALRLTGEEMEPAEEESKGLSPDALAAIVFFAIVIAFVI
                     AKSSARKNRSKRPPRSGGGVYRPPSTPRPFSGGGWKGPSGGSGGSSSGGGFGGFGGGK
                     SGGGGASGGWK"
     gene            complement(18829..19395)
                     /locus_tag="PLU15_05055"
     CDS             complement(18829..19395)
                     /locus_tag="PLU15_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOO30322.1"
                     /translation="MKKSGNVTLIVVLAIVAVLGIGFVSVSNSIVRAEEEVNASWAEV
                     QNQLQRRYDLIPNLVETVKGYAAHEEEVFTAIADARAKLGGAQSVTETAEAVNAMEGA
                     LSRLLVVVENYPELKADASFRDLTYELAGTENRIAVARQRYNTSVQSYNVKIRTFPNS
                     IVAGMRGAVPKDMFEAQAGALVAPEVKF"
     gene            complement(19520..20434)
                     /locus_tag="PLU15_05060"
     CDS             complement(19520..20434)
                     /locus_tag="PLU15_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30323.1"
                     /translation="MVEMIRSSILSATPVLLAGLGGAYTYYADVFNIAMEGMMLIGAF
                     TAVAGSYSSGSWAVGVLSGIAGGLAAALIFAFFVVSLKTDEFVTGTALNMLALGGTTY
                     SLRQLFHVKGAFMSPAIQSIPRWNIPLVSEIPCLGPILSGHPFIVYIGILLAFFVEWH
                     IFNTRFGLRLRAVGEDAIAVDSVGVSSDRIKAQAIMASGVLCGLAGAYLSLGYVTLFA
                     ENMSNGRGWIALAVIILTRGRPMGLLLMSLVFGFFDGLGLSLQGTMIPPQFTQMVPYV
                     VTIVALYFYSREKGTAKGEPEQSKASLS"
     gene            complement(20427..21509)
                     /locus_tag="PLU15_05065"
     CDS             complement(20427..21509)
                     /locus_tag="PLU15_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30324.1"
                     /translation="MKKQLLPAIIGIVVALLAGLIILWAQGYPPLQSYAALFSYSLFS
                     RFAFFSTLDKATPLILTGLSAAVGFGSNCVNLGQPGQFLIGAMAVTAFGIAVDLPAAI
                     MIPLSILVALAAGACWSGIAAGLRRRFNMDEFITTLMLNFIADYFTLYLISGPMLDTK
                     MYSPLTPMTNSGAWLPIWNGFGSAFLVAVAAFAVVSVLWNRSKIGYEWRAMGQNPIFS
                     RTGGCRNDAAYVWAMLLSGALAGLAGGLMIMGGMQHRFVKGIGANYAWDGVMIAIVAN
                     NNILATGLYALFFSVLQTGAMGMELETSVPSEFSLVLQAIVVLFVVASRESTRMLMNN
                     IAAEMRMKRARGRDKGMGDGRRASNG"
     gene            complement(21506..23068)
                     /locus_tag="PLU15_05070"
     CDS             complement(21506..23068)
                     /locus_tag="PLU15_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30325.1"
                     /translation="MQDQFIKMDSITKIYPPDTIALDDVDLVVEEGEIHSIIGENGAG
                     KSTLMKVLYGMTPPSGGEISFRGRRTRFSTPREAVAAGIGMVHQEFMLIPSYTVYENV
                     ILGAESVGRFGTLRHDDAKPKVQELVDTYGLNIDIDSRISDLSVGAQQKVEILKLLYR
                     DVDVLIMDEPTAALTPQEAGELFTRLKSLNEAGKTIIFISHKLDEVLELSDRITVMRK
                     GRRVTTVDNRGLDKAELARAMVGRDVVFSIKKTPASPEEVVFEARSLGYIDANGATRL
                     KDVSFDVRRGEIVGIAGVEGNGQYELVQLVTGLLPAQSGRVFIGGHDVTDEDVMQRRK
                     LMCYVPQDRKVSGSAQSMSLVDNAIMTHHRLNPEIEDRWKALLSSGRCRAFASRLVED
                     FGVLAPQLDAPISSLSGGNQQKVVVGREFMLDTDFILLDQPTRGLDVGSIEYVQQEVV
                     RRRDAGAGCLLISADLDELFSLSDRILVMARGRIVAELRPEATTREEIGEYMLGAKEA
                     QKAWRAGKEVGR"
     gene            complement(23248..24267)
                     /locus_tag="PLU15_05075"
     CDS             complement(23248..24267)
                     /locus_tag="PLU15_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO30326.1"
                     /translation="MKNRVVAFALAIIAILSLTLGSSLAAPPKHKVAYIINGALGDLS
                     FYDSGQEGIDRIRKELNAETRTIECNFDPVAYPQALQSAVAWGADVIFVVSYGFEDLL
                     MQYADTYPDKKWINLDTVVENKKKTITSVDFIEEEGAFMAGVVAGLVTTDTSIPGVNR
                     DKVVGAVCGDDDPVIRGFLYGYEQGAKHIDKDIEVKAIFTGTWDDPVRGKQAAKQLYS
                     QKVDIIFQIAALTGSGVLEAAKEEGKYAIGVDSNQNGIQPGHVVTSMLKHMGQSIYRV
                     YGTILDGKYKPGMVLEYGLREGGVGLAIDEHTRRILPAKSLQRVIDIENKVKSGAIKV
                     ERYRG"
     gene            24757..25947
                     /locus_tag="PLU15_05080"
     CDS             24757..25947
                     /locus_tag="PLU15_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30327.1"
                     /translation="MRSIAYIHIPFLYATAHARLEPSLGGKPIAVTKKNTVIGVSPQA
                     LATGVTPGTTRRHARQVCSEIVFASYEQDSCSELANGIWDICAEYTPLVEPIAPDGAF
                     ADFTGCGAARTIANDIMHRAENEAGIIVSIGLASSKFVAKAATPTIHNAGTCRCIPPE
                     AQQRFLDKLPVSSVWAIPSQVVAHLEKLGIKTLAQLRKVPMAELTLQLGEAGLMAYQL
                     SCGIDHSRVQAAYPEESIECSMSFESPVQTWADIVQCIAVCSSKIASKLRDSGRNASW
                     AEIYLEFASSHRNDYTSGKYRRRSFRSPTDSAYQIAHGLERIIEDQVSMPITAINARV
                     GGLVVPEEVQLSIFSSASTEPEKAQMDRALQYVRERFGAESVITANMLVVSRRDRMLA
                     ESFL"
     gene            26036..26281
                     /locus_tag="PLU15_05085"
     CDS             26036..26281
                     /locus_tag="PLU15_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOO30328.1"
                     /translation="MSKRIWAQVRVIEAQNGAPAQFYWSNRWNRVTGIVDSWRESGEW
                     WDGEEEKNYYLVSLASGGCYELCREAPGQWVLSRVLD"
     gene            26355..29591
                     /locus_tag="PLU15_05090"
     CDS             26355..29591
                     /locus_tag="PLU15_05090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOO30329.1"
                     /translation="MQPFVHLHVHSQYSFLDGADTCSTLVARAAECGMSAIAITDHNN
                     VSAAVELSKAAAKYGIKPIHGVEVSMSDGSHLTLLAQNPEGYANICRMLTEAHMSSPR
                     REPRTNMETLTKYTEGVMALSGCNRRGKIPSLVTSSRFEDAKDTAENLTSIFGKENFY
                     IELEHSLTPRALSLNAALAELAQEIGVGTVATNNVHYANKPRFEVHDALTCVRTLTTL
                     DDIHPERRINAENYLKSADEMSELFAKYPEALANTNEIADKCSPGLDLSLRLFPKYQT
                     PKGLSSAEYLVRLTREGAIAQYGKITPVIENRLSHELSIITALGFEDYFLVVWDIAQW
                     AQSQGIRYAGRGSAADSAVAYCLKLTNVDSIARGLLFERFMSLERAQKPDIDIDFESE
                     KRDDVANYIYRKYGDSHVASVCTFNTFQARSAVRDFGKALGFPPNDIDRIANVLPHVP
                     ADAVRSALQRYPEARSEEIPKWQYELLFELCESVAGFPRHIGTHLGGLVISDQPLTCV
                     TPLQMSAKGVAITQFDKNAIEDLGLIKLDILSLRTLSAVEHTVDMVRASGKGHDDFDY
                     NGIPLDDDDTYKMLNSGETIGLFQLESPAQRALQTRLGADNIEDIVASVALIRPGPIQ
                     GNMVEPFIARRSGAEEIAYIHPKLEPILKKTYGVVLYQEQVIEIATTIAGFTPGEADR
                     LRKAMTHFRSMREMEDIGRNFVAKAVANGVSPDVAETIFSYILGYAGYGFCEAHAAAF
                     ADTAYKTAYLARHYPSEFYAALLSAQPMGFYPARTLLVEAKRRGVEALPLDINHSKEK
                     YSVEGTALRVGFMQVSGISSNLIHEILHARQDDGPFMSALDFLCRIKANKTVVENLVL
                     AGAFDSTDPNRKALFWNLGRLAEVAASHRTAIDAKKTSGQASLLDIKQTSAEVRKAIP
                     SPYHSSDVSDAFRLPAAPQAYSGYSVVNLPDFSDWEKFKHETSVLGFCASRHAMEFFR
                     PALMREGVWDSRKISSADQGQIVRVAGLVVRPHRPPTKSGKTVVFLSLEDEYGLTDVT
                     VFDDVYQRQGAAIFKSPALIVTGTISRRGNGVSVIAKSIESLGEKCNR"
     gene            complement(29986..30663)
                     /locus_tag="PLU15_05095"
     CDS             complement(29986..30663)
                     /locus_tag="PLU15_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOO30330.1"
                     /translation="MAREYLALRDEGRAELIVKKSRFIATARPVDSEDAARDFIDSIR
                     KEFWDATHNVYAFTVGENDEVVRSSDDGEPAGTAGRPVLEVIRREKIKYCAVVVTRYF
                     GGTLLGAGGLVRAYSSAARDGLHAAGIVRVRLLQKVRFVIDYVALGRVQNYLAEAGYE
                     MDDVEYADRVAMTVMVPSSELDLFEARMMDLLAGDFNPEVGEEVVGFADVKEGEGLGG
                     EDGTGEE"
     gene            30766..31992
                     /locus_tag="PLU15_05100"
     CDS             30766..31992
                     /locus_tag="PLU15_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO30331.1"
                     /translation="MPTTRLYYDDPYLSDFQCNILRCEPSTRNSSPTPLYEAVTDVTA
                     FYPNSGGQPSDRGILAGADVVDVVERDGEIIHLTVGSVPLGLADGRVDIERRFDHMQQ
                     HTGQHILSAAFANLFGLDTVGFHLGDETATIDLPICEISNDQAAAAEMLACEVVFQNR
                     PVTTTFRKPCDVDESYLRKLPNNAESIRLVEIDGFDINACCGTHVRATGEVGLIKIIG
                     IERIRSVTRVEFVCGRRALADYRWKNDVVHLAASQLSIHGKELPEAMLRLQNQIRQGR
                     RGLEEALDRLRDYEAEEIYSAAVPLNPSGVRVIALISQKQDIESVRAVAQKISCKPKV
                     AAFLGCVNGKSANLVFARSNDIPLDMSELLQQALPAIDGKGGGSPAYAQGGGKNPDGI
                     PEALALVAGLAKDALG"
BASE COUNT         7679 a         9714 c         7880 g         7157 t
ORIGIN      
        1 ccgcatcttg gctttcactt agcgtgtgga atgtcgaata gcttcggcct cagttgcgaa
       61 gattcttttt gctcctgagc ggggaattgt cccctcatac tgctcttatg ataagtcgca
      121 attgcagccg ttattttcga agtatgcggc gtatcggcaa tcatccccac ccacgatgac
      181 tcgatgctcc aacacgcgtc agaatacgag gcttacggaa ttccatggat gcttcttgat
      241 attcatgcgg cagattcctc ctaaaaggcg aggtccagac atgagcattt tctagtgctt
      301 cctcatgcgc cgccttaaac gttgaactcc tcttctggca actgtttctg ggtaaataca
      361 agaacagaag cgagcgtggg cctcgcctct gcttgccatt gtcgccccgg ccaataatcg
      421 aacttctgag gaacccgccc aggcggcacg ggaacgggaa gcatccatgg cattcggatc
      481 tatccgttgt gtatatcaaa gctccagttt aggccgtaat tattgtccca gttttcggct
      541 cggtccttaa agcagaaatt gagtcgagac gtctcttcgg gaaccaacgg caaagttgcc
      601 ttccatacgc ctggcttctc ctgtaacatc gcaatgtcaa ccacatcgtg ccaacttccg
      661 gctgccccga atcctgcatg caaatagacg ctatccgcac ccgactgggc caaaaggcca
      721 ttgtatttaa ccgtgacttc ttctcctgcg gttatgggca ttggagaaac tgacactccg
      781 cctgtgagat gggtatcttt ctgggcagat ctgccagtcc ccgacctcgt gcctctttcc
      841 ttcgggctct tggcccccaa gtctgtctcc gaagcaatct ccatatcggc gcctggcatc
      901 tgccgcctct gccacaacac ccgacactaa ccccctttcg agcatagtct gccgctcgag
      961 ttcctcgctt attagcacaa atccggcctg catatggcta aaccaggacc agaaggttat
     1021 ttttgaaaaa gatccggtca agcagtgtcc aaaaagaact gccgaccgga gaatctgcgt
     1081 gctcccacac gtatggcagc aacagacgct gtcatcatat ccccgaacgc atcggtgcaa
     1141 cgtacccacg agaacaacct ccgtgcgggg attgcgggtc tccatgggtg cggtaacaag
     1201 actccgctcc cagctgtcgc gtatagtcgt cacattctct ctgcacgatc acggagttcc
     1261 ttccgcccaa agttcgcgcc ttagcttccg caaaaagcta gaacacccct taacacgcgc
     1321 tcagatcagc caataacgag tcgcagagcc cgcctgtcca cctcagacat gaccttttcc
     1381 tcgtcgatcc caatgatctt cctgtcgtcc atcacaacta ccccgtcgat caccacggtg
     1441 cttacatccg cgccacacga tgagtagaca atatgtgacg caagatccac agtggatgtc
     1501 ggcgtcagat gaggggcatc gaaatcgatg aggataatgt ccgccctctt gccaacctcc
     1561 aaagatccca cggaatcttc aagtcctagg catgcagcac cgtcaatcgt tgccatttca
     1621 aggcaagtcc gtgcaggcat ggcagtagat tcctttagag atactttgtg caacaatgca
     1681 gcgagccgca tttcctctat catgtccaaa ttgttattgc ttgcagcgcc gtcggtgcca
     1741 agtcccaccg tgattcctgc cctcatcatt ttgggtaccg gagatacacc cgaagccagt
     1801 ttcatgttgg agccaggatt gtgggcaacc cttacatcgt gctccgcaag gatctctata
     1861 tcctgatccg atacagcgac acagtgggct gccagaacag gtgcctggaa aacaccacta
     1921 gcctcgacaa accccacagg gctcatccta aattgccttt tgcagtcctc tacttcccgc
     1981 tccgtttcgg atatatgtat atggactcct acacccaaat caacagcgct ctgcgcaacc
     2041 ttggaaagga aactgcgcga gcaagtatac ggggcatgcg ggcctagcat cgttgttatc
     2101 cttcctccgg cagccccttg ccacctctgg caaaactcca cgctcttggc gagcttctca
     2161 tcgccctttc catcaagaga acccatgcca atcgagagac atgccctgat ccccgattcc
     2221 tcaacagcct tggccgcctc gtccatgaaa aaatacatgt cggcaaaggt cgtggttcca
     2281 gaccttatca tctccatgca ggcgaggaga gttccccaat atacatcctc ggccgagagc
     2341 ctagcctcta taggaaaaat cttcgtttca agccattcct gcaatggcag atcatcagca
     2401 tatcctctaa ataatgtcat tgcagcatgg gtatgcgtgt ttacgaatcc gggcatggca
     2461 attcgaccac gtccagagat tcgcccaagg ttcgcaggaa gatcattggg gacttcgtcg
     2521 accgaaccca caaacgttat cttccccgac tcggcaagca cagcgccttt gggaattatg
     2581 cgcctgtatt tgtccatggt aatcactgtt acgtcttcaa tcaacagcgc gcccattgag
     2641 cattcgcacc tcctgcttga tttcattacg ccccactttg ctctgcttcg cgactatcgg
     2701 cgggccacag aagggacggt ttgccatcgc aaaaaggctt atgccgtccc agtctgccag
     2761 cttgccatgt attgtctctg tccttcgctg agagagtcta tgcaaatacc ggctgcctcg
     2821 agcgcaagca ctgccacccg tcgatccacc tcgaccggca cagagtaaag cttcggctca
     2881 agctccttgc cgtgttccgc aatatgcaat atggagaggg cctgcacacc gaagctcatg
     2941 tccataactt caacagggtg cccatcccct gcggctaggt taaccagacg cccctctgca
     3001 agcagatata ggcgacgacc atcaggcatg cgatattcct ctatattggg ccgaacctgc
     3061 gtcgggcttc ctgttgaaat cgacgcaaga tcgggttttg gtatttctac atcgaaatgc
     3121 ccagcattgg ccagaatagc gccgtcgcgc atcctctcaa gatgctctct tcgaattacg
     3181 tcccggcatc ctgtggcggt gacgaagatt tggccttctc ccgcagcgcg ttccatgggc
     3241 attaccctga atccatccat cgacgcctcg cacgcggcta caggatctac ttcggtgact
     3301 atcacttcgg cgccaaggcc acgcgccctc atcgcaaggc cctttccgca ccaaccatac
     3361 cccgcaacca ccacacaacg gcctgctata gaaaggttcg tcgtgcgcat gatcgcatcc
     3421 cacgcagact ggccagtccc atacctgttg tcgaagagtc gcttcatcct cgcatcattc
     3481 acagccactg cgggaaaagc aagctctcca gcggcctcca tggagcaaag ccggtgcacc
     3541 ccagtcgtcg tttcttcgca cactccaagg atctgcggaa tcagctctcg tcttgccgta
     3601 tgaaccctgc ccaccaagtc accgccatca tccatgagta catcaggccg gaggtcgagg
     3661 gccatgtcga ggaattgcag gtattcttct ccggtcgccc catgccaggc atataccgag
     3721 attccgtctt cttgagcaag ggcggcagcc acatcatcct gggttgacaa tgggttgctc
     3781 ccggtcacgg ccaccctagc gccgcctgcc gcgagggcca tggcaagcct ggcggtttta
     3841 gcctccagat gacagcagac tacgatattc aaaccttcta gtgacatatc acgcgcaaat
     3901 tccttctcaa tggcctctac aacaggcatg tgagccctcg cccactctat cttgatccat
     3961 ccttcaggcg cgaggcccgc gtctctgata actgaatcgc ccatgctcta cccccactcc
     4021 tcccacgcta tctccaatgc gtctttttgt actcgtcgag ccatgggatg tcgacccccg
     4081 ctgcggcaca cccgcatccc ctctcttgcg gcagatcttc tatcgttgcc atgatgagcg
     4141 aacgcaactt ctctccgttg gcctccatga tatccacaac ttcctgatgg gtaagggggg
     4201 tcggtgagat ccccgctgcc cagtttgtaa tcatggcaat ggatgagtag catagttggg
     4261 cctcgcgggc cagcaccgtt tcgggaacgc tcgtcattcc caccaggtcg ccgcctaaag
     4321 tctcgaacat cttgatctcc gcaggagtct caaatctagg accatcggtg catacgtagc
     4381 agccgccatt gatggcttca atcccgagct ttcttgcttt ttcgtacagt acgcccctta
     4441 tctccggaca atacggcacg ccgtaatcga ggtggactac tccatgttcg cccccatcaa
     4501 agaaagtccg cacctcggat ttggcaaaat cgatgaactg atcgaaaaag acgaacatgc
     4561 ccggaggcat atcagggttg agcgagccca ccgccgacgt ggcgatgact ctttcaacgc
     4621 ccagcatctt cagagccgca atgttcgcct tgtagttgat catgtgtggc ggaacggtat
     4681 gaccgaatcc gtgccgcgcg aggaaagcga tttcctcacc ccgatattcc cctattctca
     4741 ggccagcatc gccgtaagga gtatcaatgg tctcctctct gacatttgag agtatgcgag
     4801 ggtcgtatac gccggttccg cctattatgg ctattctcat caactccacc tccacctgac
     4861 aggtcacgcc aattcgggaa gatccatgcg cgacctgtat gctaaagaac ctacttcatc
     4921 tcggagagag cctgctctaa cctatcaagc ccctccttta tcttctccag ggaagttgcg
     4981 tacgacatcc ttacgtaatc ctctgtccca aaacccgcgc cagccaccac agcgacgtgg
     5041 gccttctcca gtatggcgtc ggcaaaactt accgaatcct tcacaacttc accggccaaa
     5101 gactttccgt aataacttga taccttcgga aagcaataaa aggccccgac aggctcgaca
     5161 cattcgattc ccggcatccc actcagtctc tcgtgcatgt actccccgcg cacccggaac
     5221 tgttccacca tgtactggac aggctcatcc ggtcctgtga tcgcggccaa ggctgccctc
     5281 tgcgaaatcg aacaaggatt ggatgtgctg tggctctgga ttttgttcat ggcggaaata
     5341 agctcggaat ctcccgcagc gtagcctatc cgccaccctg tcatagcata agttttcgaa
     5401 accccgttga ccaccaatgt ccgtgccttt atctcttcgc ccaaagcggc tatggaaaca
     5461 aattcggctc ccctgtatag tagcctttca tagatctcgt ccgaaaccac aaagagatca
     5521 ttctccacaa cgatttcggc gatagccgaa agctcatcct gtgagtacat cacccccgtt
     5581 gggtttgacg gcgagttcaa gatgattgcc cgagtcctag gcgtaatcgc atccgctagc
     5641 acacggggtg tgatcttgaa atcagtggat gatgtatcga cgatcaccgg ccgagccccc
     5701 gacatcctca cctgatccgg atagctcacc cagtatggag caggaatgat gacttcgtcc
     5761 ccaggcccgc acagcacctg aaacgcattg tataggctat gcttggctcc acaggagact
     5821 atgatctgcg aaggctcata gtctagtccg ttgtcacgtc ggagtttcgc gacgatggcc
     5881 tcctttaact caggaatccc cgccgtcgga gtgtagcgag tgaatccgct gttaatcgcg
     5941 tcaattccgg catcgcggat gtgttctgga gtgtcaaagt caggctcccc ggcaccgaat
     6001 ccaatcacat cgatccccct agcacgcatc tcgcgcgcct tcgcctcaac cgcgaaagtg
     6061 ggcgacggct tgatcgccct agccctttct gacagcttca tcatcaaccc tccacttcga
     6121 atactcttat gcatcagcgt cgacttcctt cgacatcttc gaaagcagtc gcgctcccca
     6181 tcgcctcttg gcgcaaagca ggactgaaca ttcttgtgag agctccctct tgtctacccc
     6241 acttcaacat ggcacagaca ttccatccac ggatttggat gttgatcatc cagactcaaa
     6301 cgagtttgcc ttgagttgag ggcatcaaag aagttgccgg atatgaccgc cttcacagac
     6361 ccgagcattt caccaccctt cacgactatc gcctccggcg ccgccaggga aaaattacct
     6421 gagacagggt cctgggtatg catgcccaga acgctgcaaa caagcagcgc gtcctggcat
     6481 tcctcgagca agctctcggc cgctcccata ttctctgtcc tcagcaacat cccggcataa
     6541 cccggcgaca acccccgaga tggcagcgga gttggctgca tgttgcatct acgagcatac
     6601 ttcagattga gcactggcgt aacaagccga ccatccttga tcagatcggc cctgcctgag
     6661 gcgactcctt ccactgtgca cggatacgag ccagggccca ttggaacagc agtatccaaa
     6721 gctacctgca tatcgtctct tagcgccggc tcaccaccgc gaaactgtct gacctcgtac
     6781 ctggatctcc cctcagcgac gttctcccca ctcagattga aggcaatata atggccgata
     6841 aatgagtcca caacactcgg cgacatgatc acgcgcatcg ttcctgaatt gctggaagca
     6901 gggatcctgg ccgcatccac gatccttgcc gtccactcca ccgattcgtc cagcgcctgc
     6961 cacgtcccgg tcgtcctccc gtatctactg tctccgagag tcttgtcgag ataccagtag
     7021 caccatgcgc ctgtctcgga gtacttcgcc cacagcccgt ttgatgttgc cacaactgtc
     7081 tcccctgccg aacagcacac ccgggcatcc accaccattg atccactgtc tagggcagcc
     7141 tctactatcc taccgatacc ttcaaacgcc ggcgtaggat caccctggac caccccagcg
     7201 actttaggat cgaatagctt tacatcagcg ggggactcca gatcccatat gtaggcagca
     7261 tcgtcatcac ggtaggcgag ggatctccaa agatccaact cacgcggaag acggtccaag
     7321 atcttgctgt cgactctccc gcgactgcat cggccatctt cccatacaat gaagacattc
     7381 ccggaaatct ccgatgatga cctcggggga ctgtaagctc cgcccagttt cgaatcgtcg
     7441 acacctatgt ccacggattc cgtggatttc gctacgaacc gccaacctct cgcctgtgaa
     7501 agagtcttct ccagcgcctc agcaatttca gtgacagagg acatttcata ctcccccgat
     7561 cgttatctcg tcatttccat cgattacaag gaacatattc ccgcccccgc ttgaggggac
     7621 agattgcccc gatttcccgc acgtacccat cgcatctatc atcggctcgc cgatacctgc
     7681 aatgatcgag ccaagggccg agaggacctt gccgctgaat atggcgggct gacagatgcg
     7741 aatgtcatca tgcgtcagct cgtagatggc agaacaattg aagacaaaat cacccttggc
     7801 cgggttgacc tggcctccct tatatcccga aagatacagc actggctcat catctaggag
     7861 caggcccgcg cgagcaagaa catctctcac gtcttcaggc tcgacgtctt cgaattcctt
     7921 gtgaatgggc aaaggattct ccaccgttat tcgtatattg gtcatcctgg gcacgggcac
     7981 gtggcggaag ctctccgctc ttcccgctcc cgtaactgga acgcctgcgc gctcagcgga
     8041 gaatatatct gccagcccgc aacagagctg gcctcggcgg acaatgtcca cagtggcgcg
     8101 gaagatccca ttcgccgata tcggctggta cgcgtaatcc ccttcaacgg gaccatcgac
     8161 tatgttcaca atatctgatg caacgcgatg ccccttccga aacttcccgt ttagacccaa
     8221 aatcgaggtc tccattccat cgcattcagc cgcatgccca aaagcttcat gtgcaagccc
     8281 cttcgccaaa gcgtagtcta tcactaccct gtatgggccc gctgcaacgc gcggcgcatc
     8341 gagaagcgca agagcggtga gcgccgcttt ccgcgtccgc ttatcaagtc gacttcgccc
     8401 atctgaagat gcaataatac ttgcgtccgc ccctgcgagc gaggcgttag cgcttgccgc
     8461 ccgtccgccg tctctggcag taatcatgtt catcgcagcc gaccggggca tagagaacat
     8521 cgtatcggtg ccatcgcttc tgactacgcg ccactgttca ttggcgatgc ggtagaaaga
     8581 gcgaacagcc agccttggat cgatcttcat tgctgtctcg cacacctcgc ggacaaggcg
     8641 ttcctcttca tcatgggata tgctgtcaag gcccatgggg acgggcacgt cgatatcgcg
     8701 tatcaacggc ctcgcctgat ttatctcgcg agaaggctga gctccgtatt tagcagacgc
     8761 cttggccaga ttcgttgcag attcaacaag atgctcgaga gactctaagg ttatcatatc
     8821 ggacgatgca aaccccgagt aaccttcaaa agtaaagatc tgtaccccca cgcctgaaag
     8881 ctcctcgatg ctcaacaggt cagtctgccc attcattacg cccactgcac gctcagtctt
     8941 gtcctgaata cgaacgatag cataaactcc aagccttctg gccgtctcca ttgccctgtt
     9001 gcatgcctcg gtgtaggccc caaccctcaa tgtcaatcct cacatccccc agtcacatca
     9061 acttcgtcca atgccttttc ccatgcctcg tacaaagcct caagctcctc cgtgagctgc
     9121 cgatggatca gcactgcctc ccgcgcacgc tcgccgtcga catacaactc ctctgaagca
     9181 agctccgctt cgaccttgcc aacctgtgtc tccttgagtt cgatctcagc ctcaatggcc
     9241 tccacaacag cctgagcgtg accgactcca tcacgtcctg aagcgcctgc ccgtgccttg
     9301 gcacgcattg gcgccactcc cgaagccttc aacctcgcct ccagcgcagc ctcggcattt
     9361 tctgcagcaa cacgggcggc ttctgcctcg tctcgggtct ttttctcacg gtagtagcta
     9421 tagttgccgt tatacaactt ccagcggcca tcagctatct caagtatgga agtagctgtc
     9481 tggtcaagga agtatctatc atggcttgcg catatcacag tcccatcgaa ttcggcaata
     9541 gcctcttcca atgcgtggcg tgactctatg tcgaggtggt tagtgggttc atcgagcaac
     9601 aacagattag gcccattcaa gataagcttg gcaaggataa ggcgattgcg ttccccgccc
     9661 gaaagtgcct tcacgggctt gaacacgtcc tcccctcgaa aaaggaagcg cgctgcgtaa
     9721 ctcctggcct cacctaatgt ccaatctcgg gcgcggtaca gttcgtcaag gacagtcagt
     9781 gtatcatcga ggcctgcaag gtcttgccag aatatgccca aagtcacgtt ttcgccaaag
     9841 tccactgagc cggaatcagg agccagatca tctgcgatta ttcgcagaaa tgtcgtcttc
     9901 ccagagccat taggtccaac aacacccaac ctctccccgc gttccactgc aagatctaca
     9961 gcttcaaaaa gtgtcttatc tccataactc ttcgatacac cgctaacaga aacagccaaa
    10021 cggccggagc gcgccgcctt gccaaacgac agtttcattt gtgaatcttc cctgggcttc
    10081 tggatgcgtt ctattctggc aagcatcttc tcgcgacttt gcgccattgt gctgcggttt
    10141 cccgctttgt accgcctaat gtacgcctcc agcttctgta tcgtctcctg ttgccgctgg
    10201 tactcctggg cctgcctctg ccgcgctagc tccttctcag ccgcgaagaa cgtataattc
    10261 cccggatatg ccacaacctt cagatcatct atatcccata tgcgatccac cgtggcatcg
    10321 agaaagtacc tatcatggga gattactatg aaagcgccat taaaggccgc aaggtaatcc
    10381 tcaagccatt cacatgcatt cacatccaag tggttcgtgg gttcatcgag cagaagcaag
    10441 ttgggcgcag aaaccagcat gcgcgcaaga gccacgcgca ccttctggcc tccactgaga
    10501 gtatcgatga ttctctcaag gtcctcttcg acaaacccca acccgaaaag ggtagacttg
    10561 atgcgaacat cataatcata gcctccaaga agctcgtaat cggcagtgag ccgggagtac
    10621 cgtcgcatta ccctgtcaag ctcgcgctct gacgcaataa tctcgggatc ggacatccgg
    10681 ctttccagct cccgcatctc ggcccctatc tcgaacgtct tcgcaaaggc ccttttcatc
    10741 tcttcataaa ggctatttcc tgatgtgtat tctttacctt gagcgaggta ccccgcctcg
    10801 cagccggaag caagagccac agagcccgaa tcgtaatcct cgattccggc gatgatcttc
    10861 agaagcgtgc tcttgccgct cccgttccgt ccaacaaggc caatgtgatc gccttcggcg
    10921 acagacaggc agacatcttc gaatatggga gaagcaccaa aagatttctt caaggaatct
    10981 atttcaagaa ccgacaccta caccatcctc ttgtcttcga cgtgacacgc aaatgccgaa
    11041 tggttatgga tcgactccat gctttcgcac tctacctcga accatctcac tcgcggctca
    11101 ttccgcagcc gtataacaac atcccgaagt atgtcctcca cgaatttggg gttgtcatag
    11161 gcagcctcag tcacgtattt ctcatcctcg cgcttcagga gggggtaaat ctcacagctt
    11221 ccctccgact ctaggagctc gatcaagtcc tcaagccaca gaaacccggc agaactaaac
    11281 ttcacccgag cccgaatcat tgcacgctgg ttgtgggcac cgtattcgga aatctccttg
    11341 ctgcatgggc aacatgatgt gaccggaacc tcgacgccca tagcaaacga gtggcgtttg
    11401 ccgtccaaag ttccctcaaa actgcagttg taatcgagga tgctctcttt gccactgact
    11461 ggcgcaatcc ttggaacaaa atacttgaac cttatggata tgtccgcccg ttgcgctgaa
    11521 agggcctcgc acgtctcctt gaggatagcg agtatctcca catttgatat gggtttcctg
    11581 ctccacggca gaaggacttc catgaatctg ctcatgtgag tccccttaaa ctgcattgga
    11641 agtgccacag atagcgacac aattcccagc accgactggc agccgccctc tctcgcagcg
    11701 atctgcagag gcagatgcac atcctttaca ccaaccttct gtatgttgat gccccgtaaa
    11761 tccggaaagt tctgtacatc ccgcattccg gcgcctcctc tcatatcgcg ccttttccca
    11821 ttgcctaact gcttcttttt ctacgaaagc gctcattata ccttctatcg agcgcatcag
    11881 cacgacctac tacaaacact agccgattgg agccgcaaaa tccatactga attcggttca
    11941 ccccgggcat ttggtgttga gccaatagtc cgagcctctg gggacacgcg tggcgattgc
    12001 agttcacagt aattcatcgc tttcttcttc aatgccgaaa tgctcgtttc agtatagttt
    12061 cagtggcgaa agtttgtaaa agtcgataac gatggagctt gaaattccca aatggcattc
    12121 gttagagaac tgccgtaagc cgaccgccaa tcgaaaccca actacctggc tgcaggagcc
    12181 gctgaccgaa ttctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgacc
    12301 gccaatcgaa acccaactac ctggctgcag gagccgctga ccgaattctg ctcagtttct
    12361 gtgcaaatcc ccctaccgca aacccatcag tcgtgtctga aggacttcgc tcgtcctcgt
    12421 cgaaacccat tattgatcaa tcgagctgcc aagtatggag caaaaaggca gcattatcat
    12481 atcaatacgg gggtggggtt atgaaggcca tgctccttgg cgccggagaa ggtacccgac
    12541 tgagaccagt cacggcaaca cgcccgaaac ctatgatccc catagcgaat cgaccgatca
    12601 tggagcatat actcctgctg ttgaaatctc acggggtccg cgacgtatat tccaacctct
    12661 actaccttgc cgatcagata gaatcatact tcggtgacgg atctgcacta ggcctgtccc
    12721 tcaagttcaa ggtcgaagaa aaacttccag gcaccgccgg cggagtcaaa aacctcgagg
    12781 aacacttcga tgaaaccttc tgcgtcattt caggcgatct gctgacagat ttcgatctca
    12841 cacgggcgca cgaatttcac agaagccgcg gggccatcgc cacgattctt ctcacacaca
    12901 tcgataaccc tcttgaatac ggtgtggtca taacggataa agatggacgc atacagcgtt
    12961 tcctggaaaa gccggggtgg gctgaagtat tctccgacac aatcaacaca gggatctaca
    13021 tacttgaacc cgcagtcttc gatttcatcc ctccaggcgt ggacttcgac ttctcgcgtg
    13081 atctcttccc ccttctgctc cgggagggcc aaccgctctt cggatacgtg gctgacggct
    13141 actggtgcga tgtcggcaat attgacatgt atctcaaagc ccaagtagac gcgctttccg
    13201 gcaaagtcaa gcttaatatc ccgggcgagc agattacacc cggtatctgg gtgggccgat
    13261 ctgccaaaat aagttccagg gccgacctca aaggtcccat ggttttgggg gagaactgcg
    13321 aaatagcccc tggcgcaagg ctgcgcgaat tcaccgtcat cggtgataac gtaatcgtgg
    13381 cccccaactc ctttgttgta aggtcgacgg tctttagcaa ctcctacata ggcgaaaact
    13441 cgatcgtgtc gggctccata atctgcaaga atgtggtgct caaagatagc gtccgcgttg
    13501 gggaaggcgc agtaatctcc gacgattcca ttctaaaccg cagcgtcaca gtaaaacccg
    13561 gcattcgagt atggcccggc aagagcatag atgagagcgt ggtgctgtca tcgagcgtag
    13621 tatgggagca acgtctgagg cgcgagctat tccattcggg caaaatatcc gggctggtca
    13681 acttcgaatt cgctcctgaa ttcgcgctga gactgggcaa cgcgattggg ggaacgatga
    13741 agaagggtga aagcgtcata gtaagccgtg atcccagccg aacagcgagg atgatgaaac
    13801 gcgcccttgc cgccggccta tcgagctcag gagtaacagt atgcgacttg cagggtgtgc
    13861 cggcgcctat cctttctcac atggtcaaac gcggagattt tgcctatgca atacacatgc
    13921 agacctcaga cagaaactac gagctggtgg atatcaatgt gttcgattcg gaaggcctgg
    13981 cactgtcgcg cgaagcagag cgcaagctcg aaaccttgtt ggcgcgtgaa gaccctcgcc
    14041 gcacgagcgc ccgtgaagtg ggcggcatag gctactatcc cagagcaatc gatatctaca
    14101 ccgaggattg tctctcccgt atcgacgtgg aaacgacaaa agccaaccgg atgaaactcg
    14161 tagtagactg cggttatggt tttggaggaa gtatcctccc atcgatgttg gttaggatgg
    14221 gcgtggaagt cacatcgctc aatgccattg agcaggagac taagatgcca aagtccgccg
    14281 aacaattcgt agaatccctc gatgaacttg ccctaatagt caaaacggtc gatgccgaca
    14341 tgggcattgc catagacccg cagggaacga agatgtgggt aaccgatgac acaggacgcg
    14401 tattgaccga catggagcta gcctatctct tctcacttct aacagtgcgc tcgagggatt
    14461 cacggaacgt tgccatgccc agctatatgc cgtcagtatt tacacaatcg cttaccgcca
    14521 atggcgcaac ggtataccgg gctagggcta atgcaagaag ccttgccgac ctagcacgca
    14581 cgagtaagtc gtccatatca tccgacctgg ttggcggctt catctttccc aacctgcaca
    14641 acggctttga cgccatattt ggcgccgcaa agtttacaga actggtggca cgagatgccg
    14701 ctcccctctc ccaactggcg cagctcgttc ccgaacatca ccttctcttc gacactgtgc
    14761 cctgtccctg ggagctaaag ggacaggcga tgagagaact cctcagcaag ttggacgatc
    14821 ctactgtgga aacgtacgat ggatatagga gatcttttgg agatgcatgg tatatggtgc
    14881 tgcccgatcc tgtgcatcca tcctttatga tctatgccga gggcgccaca ggcgccgaag
    14941 ccgaacgaat gctctctgcc gccagggagg agatagtggc ggagatgcgc aagaacgtgt
    15001 agaagcaata tacagcacta caatgtcaaa actgggcact gaacaaatct cgatagactc
    15061 ttgcgtgatc ccccaattcg cactgctaac agttgagcct tgtctgcccg acaatggcca
    15121 aaacacttct tgcgacctgc ttaggcagga ctttgatttt ccaaaactga gatgaccagg
    15181 tcctccgtgg aaaaccgttc gtggcacatc cttgtgatgg acactgaatc ggattgactc
    15241 tcccaatccc tgagaacagg ggttttgggc tgactcaacc aaagtccatt gccaacatca
    15301 gagcatgatc ggcggtcaca ggcctttccg agcatcctgt aacgtcacca agacttgcga
    15361 catacacttg cgtagaggga aagtgtgtga tggaggtggg ttgcgtggcc aacttgcacg
    15421 ggcgccatat ctgtctgcta ggaggggata gccgcgaact gaaattagta tgcgccctca
    15481 tagaccgagg ggcaagagtc agctgttatg gcgtgccaaa agacggactg catcctgaag
    15541 caatcgcggc agcaaacgcc aaggaggcat gtcgcggagc aaacgctatt gtgctgccgc
    15601 tttcagggac agactccaac ggcaatgtaa gggttacatc cagtcctatc tgtataacac
    15661 gggaactgct ctttgtcttg agtccaaacg cgatcctgtt ttcaggaatc gtgccgcgct
    15721 ttctggccgc agaatgtcag tctctgggga ttcggactgt agaaaccaca gccaatgacg
    15781 agctcgcaat cctcaattcc attccgtctg cagagggcgc cattctcatg gccatgcagg
    15841 ccacgccctt taccatccac aatagcaact gcctcgtgct cgggttgggg cgtacgggaa
    15901 tgactcttgc tcgcatgctt catggcctcg gagcccatgt atgggcggca gcccgaaagg
    15961 cggaggatcg ggctcgcgct ttcgaaatgg ggctttcgcc cctcagcttc gcggacctgc
    16021 cttcgtgcct tccgcagata gatatcgcct tcaataccgt gcccgctctt gttctagaca
    16081 gaagcctagt gtattccatg aaaaggactg ccgccatcat agacctggca tcctccccgg
    16141 gaggagtcga cttcatcgcc gcctccgaaa ggggcatcca tgcagagctc gcccccgggc
    16201 ttccgggaaa ggtggcgcct gtcacagcag ggcagataat ctcgaaagtg ctcccggaga
    16261 tcatcgcagc agaattcgcc aaaaacaaac ccgattaggg gtgttgtgac tatttccgaa
    16321 gacaacagtc gcatcgagtg gaagctttca aggggggagg catcacgatg agcgtgacgg
    16381 tggcagtcgc agggggcggg tgggcgggat gcgcagctgc agtcgctgca gcgcaggcag
    16441 gcgcagacgt ggtgctcata gaacgcacca atcttctttt ggggaccggc ctcgtaggcg
    16501 gaataatgaa caacaacggg cgattcactg ccgcacgcga gcttgcctcg atgggaggag
    16561 gctacctcct cgatatcata gaccaagcta cactgcatcg cagaatcaac tttccaggcc
    16621 acctacatgc aagcctgtat gatgtagcaa agatagagtc gcgcgttcga gagggcttgg
    16681 agagcctcgg gatacacgtg atgcttgaat ctcgcgtcac agacgtcgag atgaacggaa
    16741 gacgcatagc acacctggtt ttggcggacg gaagccgtat aggagcagat gcattcgtgg
    16801 atgccacagg aacagctgga ccgcagggca actgcgcccg ggtcaatggc aattgcgtaa
    16861 tgtgcattct cagatgccct gccttcgggc cacgggtcag cattgcgggt aaagccgggg
    16921 caaacgaaat ctcaggaagc cttttcgagg ccatgagtgg atcctgcaaa ttggaaaaga
    16981 agtcgctatc cgtgtggcta gtaagaagcc tcgaggaaaa cggcgtgctc gtggtcccag
    17041 tcccgcggta catcgcacgc ctggacaaga cagcatcgaa ggcgtgccag cagtattcgc
    17101 tcccagaatt ctatgacaac ctggtcgtcc tcgacactgg gcacgcaaaa cttatggcgc
    17161 ccttcttccc cctcgattgc ctcaggagaa tccccgggtt cgaacacgcg gtatttcatg
    17221 atccctacgc agggggacga ggcaactcta taagatacaa catcataacc cctcacgatc
    17281 aggctatgaa agtggagggc atcgacaacc tcttctgcgc cggagagaag gtaggcctat
    17341 tggtgggaca caccgaagct ctttgcaccg ggtcacttgc cggcaataat gctgtgcggt
    17401 catgcgtagg gcaggaatct ctcgtaattc cccaatctct ggcgatagga gacttgatag
    17461 ccttcataga gcaatctctc cgaacgtcca cggggttctt gaacaagtac acctttgccg
    17521 gaggcgtcta cttcaggcgc atgtacgact caggtctcta tacgactgac gtccggcgca
    17581 tcaaccaacg tgtgaaggcc gccggcatgg atggcgtgtt caaccggaaa ttggtccaag
    17641 acaagcatcg aacagggtgg ctgcccacat aacgctgagc ggccctcggg cttctagatg
    17701 gccttggcat ttgcattgca ggccgctcag cctacacctg ctacagatat ctggagctcg
    17761 tagccggaaa cgaatcgatc agtgcgtgcg aggcaagaga acactttatc cgttgaacca
    17821 gctctggcgc tcgaggcgac ctctgccctt gttgatcccg tcaaactagt agcgggaaga
    17881 cgtcagccct aatgcgctat ttgacgccgt tgcgctattt ccatcccccg ctggcgcctc
    17941 cgccgcccga tttgcctcct ccgaacccgc cgaatccgcc tccggaggaa ctacctccag
    18001 aacctccaga cggtcccttc catcctccac cagagaaggg tctaggagta gaaggaggcc
    18061 gatacacgcc accaccggat ctgggcggtc gcttactgcg gttcttgcgt gcgctggact
    18121 tcgcaatcac aaatgcgatt acaatggcaa agaagactat cgccgccagc gcatcaggag
    18181 aaagcccttt gctctcttcc tcagcaggtt ccatttcttc gccagtaagc ctgagagcga
    18241 tcgtgttgat tgcctccacg atgcctgccg cgggatcgcc ttccacaaaa tgaggaacaa
    18301 ggttctcgtc gatcacgcgg cccaagaacg agtcaggcag atcgccttcg aggccataac
    18361 ctgtctcgaa cttaagcctt ccctcgtcct ctgctattag caaaagcagg ccgttgtcct
    18421 cttttgcctc tcctattccc catttctgaa agagttcaac cgagtattct tctatcgaat
    18481 agcctcccat atctggaacg ataacaactg caaattgcac cccgttggct ctgttcagcg
    18541 ccacgagtct ttcctcgata tcatccactt ccgaagacgt aagcatcccg gccaagtcat
    18601 tcatgagccg aggcggcacc ggttttgcag gaacctttgg ctgcccgcac acaggaatgg
    18661 agcaagccag tatcatggca aagacgcaaa caattgccgc ccatggcctc acctgggatg
    18721 tgttcatcga tcggccgaat ctgttgttac acgccgtaag ccttctcagt gttgtcattt
    18781 gcatccttcg ctccgatctg ttcccgtctg ggtcatcccc gcacatcctt agaacttgac
    18841 ctctggagca accaacgcac cagcctgggc ttcgaacata tccttgggga cagcgccgcg
    18901 cattcctgca actatcgagt tggggaaagt gcggattttc acgttgtatg actggacgga
    18961 ggtattgtac cgctgtctag caacggctat tctgttttcg gtgccggcga gctcgtatgt
    19021 gaggtctctg aaggaagcat ccgctttgag ctctgggtag ttctctacca ccacaagaag
    19081 cctggaaagc gccccttcca tggcattcac agcctcagcg gtttctgtga ctgactgagc
    19141 tccgcccagc ttcgccctgg catcagctat tgccgtaaat acctcttctt catgtgcagc
    19201 ataacctttt accgtttcca caagattcgg aataaggtca taacgacgct gaagctgatt
    19261 ttggacttca gcccaggatg cgtttacctc ttcctccgcc ctgacgatcg agtttgacac
    19321 gctcacaaat ccgatcccga gcacggccac gatcgcaaga actacaatca aggtaacatt
    19381 acctgatttc ttcaatgcaa attccccccc tcatattgat agcaggctcg attaagatta
    19441 gcatggaatt ctcggccgcg caactgccag ccaatttgcg tcttcgaggc tgattgcctc
    19501 attaaactgg ccctccacgt catgaaaggc tcgccttcga ttgctctggt tcacccttgg
    19561 cagtaccctt ctctctcgag taaaaataga gtgccacgat ggtcacgaca tagggaacca
    19621 tctgagtgaa ctgtggcgga atcatcgttc cctgcaagct cagccccagt ccatcgaaga
    19681 atccaaagac cagtgacata aggagaagtc ccattggacg gcccctggtc agtattatga
    19741 cggcgagggc aatccaacca cggccattcg acatgttttc agcaaaaagc gtgacataac
    19801 ctaaggagag atatgcgcct gcaagtccgc aaagaactcc cgatgccatt atggcctgag
    19861 ccttgatcct gtcggaactg actcctacgg agtccaccgc gatcgcatct tcgcccacag
    19921 ccctgagcct caatccgaac cttgtgttga atatatgcca ttccacaaaa aaggcaagga
    19981 gaataccgat atagacgatg aagggatgcc cgctcaaaat cggacctaaa cacgggattt
    20041 cggatacgag tgggatattc caacgcggta tcgactgaat ggccggggac atgaaagctc
    20101 ccttcacgtg aaagagctgc ctgagcgaat acgtcgtccc gcccaatgcc agcatgttta
    20161 gggcggtccc ggtaacaaac tcatcagttt tcaaagatac aacgaagaag gcaaatatga
    20221 gagctgccgc aaggccgcct gctatcccag aaagaactcc cacggcccat gatccagagg
    20281 aatagctgcc ggcgacggcc gtaaaggccc cgatcaacat catgccttcc atagcgatgt
    20341 tgaagacatc ggcgtaataa gtgtaggcgc ccccaaggcc cgcaaggagg accggggttg
    20401 ccgaaaggat gctcgacctt atcatctcaa ccattagatg cacggcgtcc atcccccatt
    20461 cccttatccc tgccgcgagc cctcttcatc ctcatctcgg cagcgatatt gttcatcagc
    20521 atacgagtcg actctctgct ggccacgaca aagagcacta ctatcgcctg aagcaccaga
    20581 gaaaactcgg aagggacaga cgtttcaagc tccatgccca ttgcgcctgt ctgaagcacc
    20641 gaaaaaaaca gggcgtacag acctgtggcc agaatgttgt tgttagccac tattgctatc
    20701 ataaccccgt cccacgcata gttagcgccg atccctttca caaaccgatg ctgcatccca
    20761 cccataatca tgagcccgcc tgcgagccca gccaaggctc cgctgagaag catagcccat
    20821 acataggcgg catcgtttcg acacccgccc gttctcgaga agatcgggtt ctgccccatc
    20881 gctcgccatt cataaccgat cttcgagcgg ttccacagaa cagagacaac cgcaaaagcc
    20941 gcaaccgcca cgagaaatgc agagccaaag ccattccata tcggaagcca tgccccggaa
    21001 ttcgtcatgg gggttagcgg agagtacatc ttcgtatcca gcataggacc ggatattaga
    21061 tacaatgtga aatagtcagc gataaagtta agcatcaaag tagtgatgaa ctcgtccatg
    21121 ttgaaacgtc gcctcaggcc tgcggcaatt ccagaccaac atgcgccggc ggcaagcgca
    21181 accaaaattg acaggggaat catgattgcc gctggaagat ccaccgctat tccaaaagcg
    21241 gtaactgcca ttgcgccgat caaaaactga cccggttggc ccaggttcac gcagttgctt
    21301 ccgaacccca cagcagctga aaggccggtc agtatcagcg gcgttgcctt gtccagagtc
    21361 gaaaagaatg caaaccttga aaacaggcta taggaaaaga gcgccgcata gctttgcaaa
    21421 ggcggatacc cttgggccca caggataatg aggcctgcca aaagggcaac gactattcca
    21481 atgattgcag gcagtaactg cttcttcacc ttccaacctc cttcccggcg cgccaggcct
    21541 tctgagcttc tttcgccccg agcatgtact cgcctatctc ctcgcgggtg gtcgcctccg
    21601 ggcgaagctc ggcaacgatc ctgcctctcg ccatcaccag tatcctgtcg gacaggctga
    21661 aaagctcgtc gagatcagca gaaatcaaca gacatcccgc tcccgcatcg cgcctgcgca
    21721 ctacttcctg ctgcacgtat tctatggagc caacatcaag cccacgcgta ggctggtcga
    21781 ggaggatgaa atccgtgtcg agcatgaact ccctgccgac caccactttc tgctgatttc
    21841 cgcctgaaag tgatgaaatg ggcgcatcaa gttggggcgc gagaaccccg aaatcctcca
    21901 ccaagcgaga agcaaaggca cggcatcgcc ccgaactgag cagggctttc cagcgatcct
    21961 caatctctgg gttgaggcgg tggtgggtca tgatggcatt atccacaagg ctcatgctct
    22021 gggcacttcc cgagactttt ctatcttgag gcacatagca catgagcttg cgcctctgca
    22081 taacatcttc atctgtcacg tcgtgacctc ctatgaagac tcttcccgac tgggccggca
    22141 ggaggcccgt aacgagttgg accaactcgt actgaccatt cccctcgacc cctgcaatcc
    22201 cgactatctc gccgcgcctg acatcgaaag agacgtcctt aagcctggtt gctccgttcg
    22261 cgtcgatgta tcctaggctc cgcgcttcaa agactacttc ttccggggag gcaggcgtct
    22321 tcttaatgga gaatacgaca tcccttccga ccatggcgcg cgcaagctcc gccttgtcca
    22381 gcccacggtt gtccacggtc gtcacccgtc tacctttgcg cattacggtg atcctgtcag
    22441 ataattcaag cacttcgtca agtttgtggc tgatgaaaat gatggtcttg cccgcctcgt
    22501 tgaggctctt caatcttgtg aaaagctccc ccgcttcctg cggagtgagc gccgcagttg
    22561 gctcgtccat gatgagaaca tcaacatccc tatagaggag cttcagtatc tccaccttct
    22621 gttgggcgcc gactgaaaga tcgctgattc ggctgtcgat gtctatgttc aggccatacg
    22681 tgtcaacaag ctcctgcact ttaggctttg catcatcatg gcggagtgtg ccgaacctcc
    22741 ctacggattc tgcgcccaag atcacgtttt catacaccgt gtaggaaggg atgagcatga
    22801 actcctgatg aaccatgcct atgcctgcgg ccacggcttc cctaggcgta gagaaccttg
    22861 tgcgccggcc ccgaaaactg atctcaccgc cgcttggcgg ggtcatgcca tagagcacct
    22921 tcatgagcgt ggatttgccg gccccattct ccccgattat ggaatggatt tctccttctt
    22981 cgacaaccaa atccacgtca tctaaggcaa tggtatcggg gggatagatc ttcgttatgg
    23041 agtccatctt tatgaattgg tcctgcaaga agcgatgcac ctccaagaaa agtctgtgga
    23101 acactgcatc tgaaataaca agggacagga agagtcggca atcagccttc ccttcctgcc
    23161 atacactgtg ttgaacgcaa ccgctgacag cacgacccgg ctagacgaag ccggcgcccg
    23221 catttgcagc ttcaatgtgc cacgcaatca tccgcgatat ctttcgacct tgatcgcgcc
    23281 gctcttcacc ttgttctcaa tatctatgac cctctgaagg ctctttgcag ggagtattct
    23341 cctggtatgc tcatcgatgg caagaccgac tccgccttcc cgcagcccgt attccaaaac
    23401 catccctggc ttgtatttgc catccaaaat cgtgccgtaa acacgataaa ttgactggcc
    23461 catgtgtttt agcatggagg ttaccacgtg cccgggctgg attccgttct ggttggaatc
    23521 gacgcctatg gcgtatttgc cttcttcttt tgcggcctca agcactccgg atcccgtgag
    23581 cgcggcgatc tggaatatga tgtcaacctt ctgcgaatag agttgcttgg cagcttgctt
    23641 gcccctgaca gggtcatccc aggtgccagt gaagatcgcc ttcacttcaa tatccttgtc
    23701 gatatgcttt gcgccctgct cgtaaccgta caaaaaacct cgtatgaccg gatcatcatc
    23761 gccgcagacg gcaccgacga ctttgtcgcg gtttacgccc gggatggatg tgtcagtcgt
    23821 tacgagtcca gcaacgacgc cggccatgaa ggcgccttct tcctcgataa aatcaacgct
    23881 ggtaatggtc ttcttcttgt tttcaaccac agtgtcgagg ttgatccact tcttatcggg
    23941 gtatgtgtcg gcgtactgca tgagaaggtc ctcaaatcca taggaaacga cgaagatcac
    24001 atccgccccc caagcgactg ccgactggag cgcctgagga tatgcaacag gatcaaagtt
    24061 gcattcgatg gtccttgttt cagcattgag ctcctttcgg atcctatcga taccttcctg
    24121 gccggaatca tagaacgaaa ggtcacccag tgcaccattt atgatgtagg caactttgtg
    24181 cttgggcgga gcggccaaag acgaacctaa tgtgagcgag agaatggcga tgatggcaag
    24241 cgcgaaagcg acaacacggt ttttcattgc ttccaattca ccctccatag aatcttgaca
    24301 cagcttggct aaggcggcaa gccccaagcc gcgccatatc agttattatc taggtctgta
    24361 ttctccactc ccgcgattac tcctgtgtac gaaatgcaag attgcacaac aagccatact
    24421 tcggtgggct ctattcttca aagtccgcac actctttaaa cgtcttcaat cggtttcgga
    24481 acgcgtggaa ctgttagtca gttatgggct gtaccgtctt acgtagcctt gaacacagct
    24541 ctacatgtgt tatcacgtgc tcataggagc cctcggcagg cctgcttata tcatcatcaa
    24601 cccactcgat caggactgat tgacggtgct gcccaatatt ttcccggaat tgcgttatgc
    24661 catccgtctc cttgactttc caatcgtccc cgggatataa tgagtctacc gaacatatgt
    24721 tctatagccg atcataaacc cggaggcgta tcgtaaatgc gctctatcgc ttacatacat
    24781 atcccttttt tatacgcaac cgcacatgcc aggctcgaac cttctcttgg cggcaagccc
    24841 atcgctgtca cgaaaaagaa cacggttata ggcgtgtcac cccaggcgct tgcgactgga
    24901 gtaactcccg gcactacgcg aaggcatgca aggcaggtat gttccgagat cgtgttcgca
    24961 agctatgaac aggattcatg ctccgagctt gccaacggaa tatgggacat ctgcgccgag
    25021 tacaccccgc tggttgaacc catagcgcct gacggcgctt tcgcagattt caccgggtgc
    25081 ggcgcagccc gaacaatcgc caacgacatc atgcacagag cggaaaatga ggcaggcatc
    25141 atcgtatcaa taggccttgc aagctccaaa ttcgtagcaa aagctgcaac gcctacgatc
    25201 cacaatgcgg gaacatgccg gtgcataccg ccagaagccc agcagcgctt tcttgataag
    25261 ctcccagtga gctctgtatg ggctatcccg tctcaggttg tggcgcacct tgaaaagctc
    25321 ggcataaaaa cccttgctca actgcgaaag gttcctatgg ctgaactcac gttgcagtta
    25381 ggcgaagcag gcctcatggc ctatcagctc agctgcggga tagatcactc acgggttcag
    25441 gccgcatatc ccgaagaatc catagagtgc tccatgtcgt tcgaatctcc cgtgcagacc
    25501 tgggccgaca tagtccagtg catcgcagtt tgctcatcca agatcgcctc caagctgcgt
    25561 gactccggaa gaaacgcatc atgggcagag atctacctgg agtttgcaag cagccatcgc
    25621 aatgactaca ccagcggcaa atacagacgg cgctctttcc gctctcctac cgattctgca
    25681 taccagatcg cccacgggct tgagcggatc atcgaagacc aggtttcgat gcctatcaca
    25741 gccattaacg cgcgcgtcgg cgggcttgtc gttcccgaag aggtgcagct ttccatcttc
    25801 agttcggcat ctactgaacc cgaaaaggcc caaatggacc gggccctcca gtatgtgcgc
    25861 gaacgattcg gagcagaaag cgtaatcacc gcaaacatgc tcgtagtatc aaggcgcgat
    25921 cggatgcttg cagaatcgtt cctataaacc ccatatgcac gtgtacgctt aataggtgcc
    25981 cacagctggc aacctgtagt ggtctacggc aatattctgg gagtgatggg gctttatgag
    26041 taaacgcata tgggcgcaag ttcgtgtgat tgaggctcaa aatggtgcgc ccgcccagtt
    26101 ttactggtcc aacaggtgga accgggtgac cggcatagtc gactcctggc gggaatcggg
    26161 agaatggtgg gacggcgagg aagaaaagaa ttactatctt gtctctttgg catcaggcgg
    26221 atgttacgaa ctatgccgcg aagcgcccgg ccagtgggtg ctttcacgag tgctagatta
    26281 gcctcctcgc tgttgcgatc gggagtagac cgccgagaag atccatcaca tctttgacta
    26341 tggaggcaat acccgtgcaa cccttcgtcc atctacacgt tcactcgcaa tactcctttc
    26401 tggacggagc cgatacttgc agcacactag tggctcgcgc cgcagagtgc ggtatgtctg
    26461 caatagccat aaccgatcat aacaatgtaa gcgcagcagt agagttatcg aaagccgctg
    26521 caaaatacgg gataaagcct atacatggag tcgaggtttc catgtcagat ggctcgcatc
    26581 tgactcttct ggcccaaaac cccgagggtt atgccaacat atgtcgcatg ctcactgagg
    26641 cacatatgtc ctccccccgc cgcgagccaa gaaccaacat ggagaccttg acaaagtata
    26701 ctgaaggcgt aatggctctg tcaggatgca accgccgggg caagattcca tcccttgtca
    26761 catccagcag gtttgaggac gccaaagaca cagcagaaaa cctaacctcc atattcggca
    26821 aggagaactt ctatatcgaa cttgaacatt ccctgacacc tagagcatta tccctcaatg
    26881 ccgccttggc agagcttgcg caagagatcg gagtgggaac agtcgccaca aacaatgtcc
    26941 attacgccaa caaacctcgt tttgaagtgc atgacgccct tacatgcgta cggacgctca
    27001 ccaccctaga tgacatacat ccagagcgcc ggataaacgc ggagaactat ctgaaatcag
    27061 ctgatgaaat gtccgagctt ttcgctaaat atcccgaagc actggcaaac accaatgaga
    27121 tcgctgacaa atgctcaccc gggctcgatt tatcgctgcg ccttttcccg aagtatcaga
    27181 caccaaaagg cctctcatca gccgaatacc tcgtgcgcct cacacgggaa ggcgccatag
    27241 cccagtatgg aaagatcact cctgtgattg aaaaccgcct aagtcatgag cttagcataa
    27301 tcaccgccct cgggttcgaa gactacttcc tcgtggtgtg ggatatcgcg cagtgggccc
    27361 aatcgcaagg aatccgttat gcagggcgcg gatctgcggc tgactcagcc gtcgcatact
    27421 gtctgaagct gacgaacgtc gactccatag cccgaggcct cctctttgaa agattcatga
    27481 gcctggagcg cgcccaaaag cccgacatcg acatcgattt cgagtccgaa aaacgtgatg
    27541 acgtggctaa ttacatatac cgtaaatacg gcgacagcca tgttgcatcg gtctgcacgt
    27601 tcaacacatt ccaggcccga tcggccgtac gcgacttcgg caaagcccta ggatttcccc
    27661 caaacgacat cgataggatc gccaatgtcc tcccccatgt acctgccgat gccgtgcgca
    27721 gtgccctcca gcgctatcct gaggcacgca gtgaagagat accaaagtgg cagtacgagc
    27781 tcctgtttga actatgcgaa tcagttgcag gcttcccgcg gcacatcggc acccacctgg
    27841 gcggccttgt gatttccgac caaccgctca cgtgcgtcac gccgcttcag atgtcagcca
    27901 aaggcgtagc tataacgcag ttcgataaga acgccataga agatctcggc cttatcaagc
    27961 tagacattct ctccctgcgc accctctccg ctgtagagca cactgtcgac atggtgagag
    28021 cttctggtaa ggggcatgac gacttcgact acaacggcat cccattggat gacgatgaca
    28081 cttacaagat gctgaacagc ggtgaaacca tcggcctatt ccaactggaa agccccgctc
    28141 agcgcgcgct ccagacacga cttggagccg acaacataga agacatagta gccagcgtcg
    28201 cccttatccg cccaggccct atccaaggca acatggtaga accattcatc gcgcggcgca
    28261 gcggcgccga ggaaatcgcc tacatccacc ccaaacttga accaattctg aagaagactt
    28321 acggggtagt cctctatcag gagcaggtga tcgagatcgc cacaacgata gccggtttca
    28381 cccccggcga agccgacagg ctccggaaag caatgacgca tttccgttcc atgagggaaa
    28441 tggaggatat aggccgcaac tttgtggcga aagccgttgc aaatggagtc tcacctgatg
    28501 tagctgagac gatattctct tacatattag gttacgcagg ctatggtttc tgcgaggcgc
    28561 acgccgcagc cttcgccgac actgcttaca aaaccgcata ccttgcgcgc cactacccat
    28621 ccgaatttta cgctgcactt ctcagcgcac agccaatggg cttctatcct gcccgaaccc
    28681 tgttggtaga agcaaagcga cgcggggtag aagcgcttcc ccttgatata aaccacagta
    28741 aggagaagta ttctgtggaa ggcacagctt tgcgggtagg gttcatgcag gtatccggca
    28801 tctcctccaa cctgatccat gagatcctac atgctcgtca ggatgacggg ccattcatgt
    28861 cagccctgga tttcctatgc cgaatcaagg caaacaagac cgtcgtcgaa aatctggttc
    28921 ttgccggagc cttcgattcg actgacccga atcgcaaagc cctcttttgg aacctcggtc
    28981 gccttgcgga agtcgctgcc tcccatcgca cagccataga tgccaagaag acttccgggc
    29041 aagcaagtct gctggacatc aaacaaacca gcgccgaagt gcgcaaggcg atccccagcc
    29101 cataccattc ttccgacgtc tctgatgcct tccggctacc tgccgcccct caggcctact
    29161 caggctattc agttgtcaat ctccctgatt tctccgattg ggagaagttc aagcatgaaa
    29221 catcggtact cggcttttgc gcatctcgcc atgccatgga gttcttccgc cctgcgctca
    29281 tgcgcgaagg ggtttgggat tcgcgcaaga tctcatcagc agatcaaggg caaatcgtgc
    29341 gcgtggcagg gcttgtagtc cgcccgcaca ggccacctac gaaaagcggc aaaaccgttg
    29401 tcttcctctc gctcgaagat gaatacgggc tcacagatgt cacagtcttt gatgatgtat
    29461 accagaggca aggcgccgca atattcaaaa gccctgcact aatcgtcacg ggaacgatct
    29521 cgcgccgggg caatggggtt tcagtcatag ctaagtccat agaaagtctc ggggagaaat
    29581 gcaaccgcta gtaggcaagc tgagtaactt gcgagaagcg tcttatagat taggcggaca
    29641 aagtctgaac tgtaggtaat acggactcga ggatcgcgca agagcttatc acgacattcg
    29701 acgcatgctg catgaaaacg aagcagcgta atggaaagcc ctgagctact tccgccgcct
    29761 gaggccacag cccaatgcta agggcgagaa tttgaggatg atcgaataac ccatattggg
    29821 cattccgcac taagaacgtg aagagaaacg agcatgcagc tgcgtccaac cccatgcaca
    29881 gctgctcagc cacatatctt gcagcttttc ccggtaggcc tgcggcgtgg ccaaaacggc
    29941 gctaccgtcc ccatcctcac cctcatcccg atccacatta ctctactact cttctcccgt
    30001 cccgtcctcc ccgcccaaac cctctccctc cttcacatca gcaaacccta cgacctcctc
    30061 ccccacctca gggttgaagt cgcccgcaag caagtccatc atcctagctt caaacagatc
    30121 cagttccgac gacggaacca taaccgtcat cgcaacccga tccgcatatt ccacatcatc
    30181 catctcatac ccggcttccg ccaagtaatt ctggactctg cccaaagcca cgtagtctat
    30241 gacgaaccga accttctgta aaagcctgac ccttacgatg ccggctgcat gcaacccatc
    30301 cctagcggcg gacgaataag ccctcacaag cccgcccgcc ccgagaagcg tgccgccgaa
    30361 atagcgggtt acaacgactg cgcagtactt tatcttctct cgccggatga cctccaacac
    30421 gggccttccg gccgttccgg caggctcgcc atcatcgctc gaccgaacca cctcgtcgtt
    30481 ctctccgaca gtaaaggcgt atacattgtg agtggcatcc caaaactcct tacggatcga
    30541 atctataaag tctcgggccg cgtcctcgct gtccacagga cgtgccgtag cgatgaacct
    30601 gctttttttt acgattagct ccgctcggcc ctcatcccga agggcaagat actcacgtgc
    30661 catcatcacc actccttcga ccagataaaa gggaatcgag ctcggcagta gaagtaatca
    30721 tctgcaaccg gagctcatca gagcttgagg aggcgaattc ttcaggtgcc tacaacaagg
    30781 ttatactatg atgatcctta tcttagcgat ttccaatgca atatcctccg ctgcgagccc
    30841 tcaactcgca attcctcccc cacccctctg tatgaggcag tcaccgacgt caccgcattc
    30901 taccccaact ccggcggaca accctcagac cgcgggatcc tggccggcgc cgacgtagtc
    30961 gatgtagtgg agcgggacgg agagataatt cacctcacag ttggatcagt accactcggc
    31021 ctcgcagacg ggcgagtgga catcgagcgc aggttcgatc acatgcagca acatacaggc
    31081 cagcacattc tctcggcagc attcgccaac ctgtttggcc tcgataccgt cggatttcac
    31141 ctcggcgacg agaccgcaac tatagacctt cccatatgcg aaatcagcaa tgatcaggcg
    31201 gctgcagctg aaatgctcgc atgcgaggtg gtgttccaaa acaggccagt cacgaccacg
    31261 ttccgaaagc cctgcgatgt agatgaatca tacctccgca aacttcccaa taacgccgag
    31321 agcattcgac tcgtcgagat cgacggtttt gatatcaatg cgtgttgcgg cacgcacgtc
    31381 cgggccaccg gcgaggtcgg tctgataaag ataatcggca tcgaacgcat tcgaagcgtt
    31441 acccgtgtcg agttcgtctg cggccggaga gccctggccg actaccgctg gaaaaatgat
    31501 gtggtgcacc tcgccgcatc tcagctttca attcacggaa aagaactgcc agaggcgatg
    31561 ctccgcctcc aaaaccagat ccgccaaggg cgacgaggtc tagaagaggc cctagatagg
    31621 ctccgcgact atgaagcgga agagatatac tccgccgctg tgcctctgaa cccatctggc
    31681 gtgagagtga tcgcgctcat aagccagaaa caggacattg aatccgtccg ggcggtagcg
    31741 cagaagatct cctgcaagcc gaaagtcgcc gccttcttgg gctgtgtcaa tgggaagtca
    31801 gccaatttgg tcttcgccag gtcaaacgac atccccctcg acatgtcgga gcttctccaa
    31861 caagcactcc ctgccataga tggcaaaggt ggcggctcac ctgcttatgc tcagggcggc
    31921 gggaaaaacc ctgacggaat tcccgaagcc ctcgccttgg ttgcaggcct ggcgaaggac
    31981 gccttgggat aaggccctgt gattcaacca agtcgatagg tagccataac ccgagcaaaa
    32041 tgatactggc aggtcatgac gtccattcaa aaggcgcggc cgacttgcgc acaggcaaac
    32101 gaggcaacag tacccccaac ggaagtcatg acctaatcga catcagattg cctgactcgt
    32161 agcggcgcag tcctgtttga aacaatacct ttgctgctac agcgctgccc gctgcaaagg
    32221 caatcagtcc cgcaagaagc ggccaactca aattgccgag aggctcaacg ggaacatatg
    32281 caaaaagcct actggtatca caatgtccgg acagcgactt caaacagtga cccagggtat
    32341 ccggagaagg tgatcattgc ctggggcatc tggacgctga cagtctgggc atttcatata
    32401 aaaaacgagg ctgccagcga aagttgcaaa gctggcgtaa acagccgccg ctgcaagact
    32461 tgccgcctca tgagactagg aatctgctcg taaatagagc cgatatcagc gccacgggat
    32521 ctttggcaat
//
LOCUS       DAOSYL010000021        32161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1134_length_32161_cov_7.447362, whole genome shotgun sequence.
ACCESSION   DAOSYL010000021 DAOSYL010000000
VERSION     DAOSYL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 32161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32161
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1134_length_32161_cov_7.447362"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1842
                     /locus_tag="PLU15_05105"
     CDS             229..1842
                     /locus_tag="PLU15_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOO30332.1"
                     /translation="MLSRLARKIMRAIAGDRQRGKNIISSEGDSAQLSSDLENNIAYI
                     VKAFGSSSDLVVRRLRAGKGGSVSAAVIHLDGLVDGLLVAQSVVRSITVMTESLDSPE
                     SVLDELHDGLISVTGVDKVVDGYEATLRIAEGSCVVLIDSLPTALACAVQGWEQRSPE
                     EPNTEATVRGSKEGFVESIRVNTSILRRRIAHPRLRIEECRLGRLTRTSVVIAYVQGI
                     ARPSIIEEVRSRLAAIDIDSIQESGQLEELIEDAPFSPFPTIARTERPDRIVGALLEG
                     RVAILLDGTPFALIVPVTLTMLLSTPEDYFERFYAGTLIRLIRVLSVFVSLLMPSFYV
                     AVTTYHQEMLPTSLILSIAAQREGVPFPAFLEAFIMEVMFELIREAGIRLPRVIGPAI
                     SIVGVLVLGDAAIRAGLVSPIMVMVVAATGIASLATPAFSLAIGIRVLRFAFLFLAAF
                     LGFFGIAIGALALFAYLTSLESFGVPYMSPIAPVIDAGLRDAILRVPWRAMKERPEWL
                     TWYNQTRMDSAANHKDEHSTFREGRRKEQ"
     gene            1845..4232
                     /locus_tag="PLU15_05110"
     CDS             1845..4232
                     /locus_tag="PLU15_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOO30333.1"
                     /translation="MSHKLTARQLGSILFLQRALPVTLTFPMMIGMQSSHGLWIAGAL
                     GTLISLPLLVWMASLSRAEGLDDIIEISRRRLGTTVGGAVGWLFVFYWMLLAALQVRS
                     VGEAYVIGTMPETPIVVFMVLTALVSSGIARRGIKLIAMMSELTAVLILLGLLLTFTL
                     PADVMQFRNLLPLLPEDLSSLALPTGTAVSLFLDLNVLMMIAPYMKSGRDLMRGTVYS
                     ALISGAILILLAVVVTAVFGPLATSLELPALSLTRMISLGEFFERLELITVASWTSGA
                     GLVLSTSLWAAAEASANLLGLKRYEPLVYPLGGLAVIMGLGMWPNMGAFDRSASAKSG
                     SLVTAVFIIAVLVVLTGARWLNRRKGEGPGGTRMIAAILALGLTAFLATGCWSHREIE
                     SLGFVNAVGVDTALGKTHWELPGEERDPGELIQVTAHVVKPSAIVSGERGPAPEKPFW
                     VISATGYTIFEAVRNVSELSPRRLSWPHSRWVLFGEEFAKGGVARAVDFLVRDQETRR
                     RAVLGVASGARAWDLLQSEFELERVPGEAGMGIAMNASKSTSTIVIASVNDFVMALES
                     EGIDPIALRIEVMPYTYPYEITGDVTREQIKSVARLTGAAVFRSDKLVGWLDGREARG
                     YNWITGKTKSGILVIDAPEPSLGRASLGSRVGLEIIRSSGSFRPKIEDNGRTIGIVIK
                     IKAEANISDVQPYVDLYAHPGLWESMERLMAKAIEDEIMAAVKKAQDLRADVFGFGRE
                     IHRTRPKLWKEIRNGWYDIFAEIEPEIEIEATLVRSGLTVRSVKLNEMGGSGGQQ"
     gene            4229..4447
                     /locus_tag="PLU15_05115"
     CDS             4229..4447
                     /locus_tag="PLU15_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30334.1"
                     /translation="MNIVLLVLAVVVLGFYEAPDLIRNKLWRELAVFVFAVGLDFTLA
                     LLMLLGVNIPNPAIWIDKAAKWMLSLFR"
     gene            5438..6103
                     /locus_tag="PLU15_05120"
     CDS             5438..6103
                     /locus_tag="PLU15_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOO30335.1"
                     /translation="MKLYKGRWSDIMVGRALLFAGLVAVAGYVVVTVVGAEMIPGYNH
                     LSHPLSEMTAQGSATKPALDRWFILLDTMVIGFASVMPASVCTQNDGVATYVQRGAAF
                     LALAGVLGIIMTLWFPMDGSILVTTVVGTLHEFIAAAIMVCSMLAILLFGFGLRNSSE
                     WGGFAFYSFATFAFAGSCSIVTAHAFLLGTDLQGAWQRLTAGAILQWIFVVSLRALSA
                     PMD"
     gene            6755..7798
                     /locus_tag="PLU15_05125"
     CDS             6755..7798
                     /locus_tag="PLU15_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOO30336.1"
                     /translation="MGNEKDRKTGLSVEALLAVFIALACILTVFAIANQRRSSRVEIS
                     DLPERVRFAVLCTMDANAGGTKSVAWAPDSQRIVTGEGDGLVVVWNARCGEKRLTLHG
                     RPGYQIENVCWSPDGKHIAAITGEDEFTVWDSGSGEVEWTVVDPEVYGLEGWSEDGKS
                     VTTWNGEEYFSSWNIESKTMENRWFVSIGEQSSVPSPDGKLAAWQVGEDVWAIHTVSS
                     EKFDTKELCGHSGFVRHVSWSPDSSRLAGATDEGIVVWNVETGQLEKEHKRAAGGFAN
                     VLWSPDGRHILAYTDDGSGVIFDAEQVDPVADISVDGRRWWSLPVAWSPDGSLLAAIT
                     QDQTVLIYGQITY"
     gene            complement(8040..8876)
                     /gene="fetB"
                     /locus_tag="PLU15_05130"
     CDS             complement(8040..8876)
                     /gene="fetB"
                     /locus_tag="PLU15_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HOO30337.1"
                     /translation="MDANSYIHISNMDLLIALLFIAISGAISRWQKLELERDMAVGTI
                     RAFAQLLAVGFVLQQLFDAARWYWVVLALAIMTTVAAHNGVRRQTGAKQGLFTVMMAA
                     IATGASITLILVIGVVLRVRPWYMPQYVIPIAGMILGNCMNGAALVVNRLHSELTLRR
                     NQVEAALALGAPAKQAASEAMRESLRAAMMPTINSMMTVGLVHLPGMMTGQMISGTSP
                     LDAVRYQIVVMLMIAAATAVTAMTAAFGALGVFFTPFHQLSPRLELGEAWDAYTHDKS
                     KH"
     gene            complement(8866..9630)
                     /locus_tag="PLU15_05135"
     CDS             complement(8866..9630)
                     /locus_tag="PLU15_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO30338.1"
                     /translation="MDSMTCVPAIELNRVSMTKPVLLGNSLTELDIIRDVSFSVPEGE
                     ILALIGPSGSGKSTLMRMINRMEDPTSGQILLFGSDISGLDVRELRRRVGMVSQTPAL
                     LPGKVSDNIVYGPSLRGEPCNPEEYLELVGLEPELVSRPASSLSVGQQQRMCIARALA
                     NRPNILLLDEPTSALDRTAARNIIELICNLNRQLGLTVIIVTHVMEHAQAIATRVAML
                     VGGVLIEEGAAADFFEGPKTQIGHRFLKGELTEDGR"
     gene            10850..11851
                     /locus_tag="PLU15_05140"
     CDS             10850..11851
                     /locus_tag="PLU15_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO30339.1"
                     /translation="MRALVFKDGLREVDMPIPEITMGQALVRVTLAGICNTDIEVTRG
                     YFAYTGIMGHEFVGVVEDADDPDLIGKRVVGEINVSCGECPTCRGGNPRHCEHVKAMG
                     INGWPGCFADYVVLPECNLHVVPDSVSDHQAVFVEPLAAGVEVMEQVHIRPSDEVVVI
                     GDGKLGLLTAQAIHACGNRVTVLGKHVEKLQIARDLGMDVSLVADFVDTVDVVVDCTG
                     NPSGMETAMAMVRPKGTIVLKSTFKQGAPLNLTPIVRNEVTCVGSRCGPFPPALRLLE
                     TGAIVTEPLIEAIYPAHQMEAAFERACTRGSLKVLIDFTCSQGSGEVGAGKMERLHF"
     gene            12340..15402
                     /locus_tag="PLU15_05145"
     CDS             12340..15402
                     /locus_tag="PLU15_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOO30340.1"
                     /translation="MRKLYGVLTVCTLVAMALWLAPASVGSGEPLYVALIWHNHQPFY
                     KNTATGMYMMPWVRMHAVKDYYDMASILEDYPNVHVTFNLVPSLILQLDEYADGAQDV
                     QLLLSLKPASELTDDDKDFILRRFFDANWDNIIKKYPRYWQLLNKRGQGVTDESIAAA
                     IAAYDEQDFRDLQVWFNLAWLDPDFIDEDPSIEALVRKGRGFTEEDKRVVLDKHNEII
                     RNVIPIYREMQNAGQIEVTTTPFYHPIMPLIYDVDLARVASPKLELPSEPFAYPEDVE
                     AQLSKAVDFYKDHFAVAPRGLWPSEQAVGQDIVGIVHEAGFQWMVSSEGVLAQSLGIG
                     LRDGAGRIVRPDVLYKPYIVEQDGKEVTILFRDIVLSDKIGFSYSGMSGRAAASDLTD
                     YLRSVKRSLASTPGPHVVTIALDGENCWEYYENDGKEFLHTLYEALNDDPDFKAVTVE
                     EYLAAHPATERIPMLHTGSWISDDLETWIGEVEENRAWDYLSKARTALAGFTAEHADS
                     ADDEMMTALERAWEELYAAEGSDWFWWYGDDQDSGNDEAFDELFRIHLQNIYSYIGLE
                     VPNYLYMPIIPKRAAVPEERITGLMHEPVMDGFIDWGEWEKSSFYPKDDAAVFSWGEG
                     ENIFSSLRVGVDNENLYIRIRTQDGFDELYGKDIKIAAYLTNPRRHEVNTIPRGSASG
                     RDATVLGFAPGTEILVDFAGIERAGRATALLSVAAGDDTWTEVSRINTVGVGEALEIS
                     VPFEVLRMKPADVAHIVVVASSKDQNIDMIPTSGPAEFKAPVVTVGRRLFRVEDPRGD
                     DHGPGTYSYPTSPVFTPGAFDMVSCEVIDSGEEVVFNVELAGEITNPWNSGIGLSVQT
                     IDIYIDTDNKVSSGSTTALGGRRVEFEPECAWEYAIWVEGWNQRVFMPEGGDVGGVTA
                     TVDTIGNLVSISVPKSIIGQPEPGWGYQVFVLGQEGYPSEGNLRVREVMEQAAEWRFG
                     GGDNSVYDPNVIDMLVPTGRTQEEILSAYDVSAKKLAVVPMIYPDWEY"
     gene            15495..17621
                     /locus_tag="PLU15_05150"
     CDS             15495..17621
                     /locus_tag="PLU15_05150"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30341.1"
                     /translation="MSERVSFWSAIIVVLALASPAFAAERIPVAFSFTPPMAANTVAV
                     AGTFNDWNPTANLMIGPDEDGIWTAVIHLQPGRYEYKFVVDGKTWHHDPNAVEQVADP
                     YGGKNSVVYVGVVPVAESSIAFDGWFESKLAKEAGEPLSLSGDAYLKFSGELRPCLET
                     YTELHAWKSLSLGGDAGWHLMPPIDGLEVNQSVATFRLSDGIAAKLFYRGHAGNTKDP
                     LTLIASEGSDNRIWDRRAAEILCFKGIVDGRIGAATLAGNKTLVYGEMEAGLTDRITL
                     GGVATYERWATSAREDGEDHRANIGAYAVAKPTEALTVRGQLLQTHGTKIVSDSTVPV
                     EVEFVYPAERESSGADVIYIVGSFNNWSTEDGLPLAKDADGNWRGSIELLEGEYEYKF
                     WYPDPDNDPNWGHWMGQGTGGDNLVRHVGGDGPSFQDFYFVEPAEHQVYIRGSWDWSK
                     DHELVRDPLTGYWKHTMRLDPGRYEYVFHYNDGSQDHWFGQGGDNVRFVAGAGGREAD
                     YSALAYFAEASYQADRYGVTLGLKGAQDGVVASYGHVGADYRTVYGKAWAQAAEKIKL
                     TLDTSYSTDAAGTKDSATLTVRPGLEILEPAQHVAFLKGSYEYRKGKSAGGVASLSTR
                     IGSAGLAAEFAHAPLGPSSLKLSVANDFPYDLSAKAEYWKKLKTDDYSLTIEATKKID
                     WCDGTTIGVSCALPGRKWGAKLSVDF"
     assembly_gap    17717..17816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17817..18746
                     /locus_tag="PLU15_05155"
     CDS             <17817..18746
                     /locus_tag="PLU15_05155"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOO30342.1"
                     /translation="RYGGGCKREALMCRIETGLPKGDNIMARFGIHLSISGGLTNATS
                     RAVELGCDCFQIFSRNPRTLKAKPLDPGEANSFRAAVVEAGLKPVVIHANYLINVASP
                     KDETYEKSVSALTDELRRAEILGADCLVLHPGNHLGSGVEAGSRRIASAIDRAYEDSG
                     ALVPICLENMAGAGTEIGVSFSELRSIIDISERGPFLRICLDTCHAYGGGYNVATERG
                     LDATLGELDSAVGLDRVMIVHANDSKGQLGSGKDRHDHIGEGSIGLAGFRAILARPEL
                     RRLPFVLETPVDAPEDQVRDLAALKACAAGEER"
     gene            18743..21418
                     /locus_tag="PLU15_05160"
     CDS             18743..21418
                     /locus_tag="PLU15_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOO30343.1"
                     /translation="MKSGALVGAVSNYIASEAAESLRQAIAEAGGTEVFAVGRCDRDF
                     IVTEISVLARGNLTAAPVVDATLMHGQVVIHNHPSGKLLPSEADLDVAARLAARGVGF
                     WIVNNDVSQIRSVTDPLANQAGSIVIDPADIRHIFSLDGPLSAAFEDYEVRQGQIDMA
                     LAVSSAFSDSACLVVEAGTGTGKSLAYLVPAFLWAKRNNARVVVSTNTINLQEQLIQK
                     DIPALEAAIGEPLPAALVKGRGNYLCMRKLNRVLEDEESSIEPEYRDGFAQVLQWAME
                     TETGDRSDMPFEPDSEVWGMVCSEGDMCLRTKCPNYGQCCFHRARQAMEKAEVLVVNH
                     HILFADAFIRSQMGPEADRAVLPRYNVVIVDEAHNAADVATQHFGRQVSRVSLVRLTN
                     SIYRRERAAAQSAGTASTGDYGALVRLRSSLYSSWPGESGRARAEACLDLIHIDAIPA
                     VIRAREAGEAFFDEVRRMLMDVPGDGVERTLRITGDVKQREDWDARIFPAHERFAAAL
                     EGLADCLERLSVLLRGDDDEALLESAKRLTFEAESGSGVAADEDGVDLGPGFDEADFP
                     DIVELAAYAGRAREFASSLRFAVTADAPGHVFWAEASGAGPRSNVRVVAAPIEAGKAI
                     AEHVLGTQESVVFTSATLAVGTSFDYIIHDLGLDLLDGDRVRQMAIAAPFDYGRQALL
                     AVVDDIPNPDSHGWEAAVAEAVASILDASDGRAFVLFTSYKLLSEVARAVEPRLAQRN
                     IALLRQGNAPRHALLAEFRRDVRSVLFGTDSFWEGVDVPGEALSCVVMPRLPFAVPTA
                     PVVEARIEHIAESGGNPFNQYTLPAAVLKFKQGFGRLVRSSSDRGAVVVLDSRIVRRH
                     YGRKFIDALPGCAKYVGGFEDVSERLYEWLINPSP"
     gene            21614..22462
                     /locus_tag="PLU15_05165"
     CDS             21614..22462
                     /locus_tag="PLU15_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO30344.1"
                     /translation="MSKWRTLLDVACVAFVLSFVLTLFGNATAEVGMGVISAVSTGGR
                     LVPIYSVARDDKTVSVTVDATWGDDKTLALLDLFDKHEIKVTFFLAGNWIKSYPEKVK
                     AIAARGHEVGNHSWTHPHMGGLSSAQIREELERTQSALCDLTGVRPTVFRPPFGEYTN
                     SVITIAEECGCKTIQWSIDSLDWKDLTADQIYDRVMKRVGPGSIILFHNAGKHTPEAL
                     DRIIRDLKAQGYAVVPVSELIYSSDYYIDRNTGEQRPVKVRTRAEPSLPQQSPPTSDS
                     SSGLRR"
     gene            22558..23556
                     /locus_tag="PLU15_05170"
     CDS             22558..23556
                     /locus_tag="PLU15_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO30345.1"
                     /translation="MIFITIRRRTLILIVLGVILAIVAACIVVSMPRMRRAALGAKPG
                     VRVRDVDVGGLYKEEVRSIVLGLAGKVERSPKEAMYHAETGEIVPEEPGIIVDIDNTV
                     TSVMAAPRGAVLDLVTRQVPPAVTAAMLKPIYRVDTSEPAVALAFNVAWGEEWVPAIL
                     EILAEAGAKSTFFITGTWAAKFPHLVQAMVAAGNEIANHGYDHPHPEKLSESELVALI
                     EKNETLLRELTGVRTRLFAPPYGEVNQRIAAVAGRLGYATIMWTVDTIDWQRPEPGVI
                     VERAERRLAGGNIILVHPTEPTVAALPVILERFAEKGYKVVTVSRLLELGPAVHTR"
     gene            complement(23570..24907)
                     /locus_tag="PLU15_05175"
     CDS             complement(23570..24907)
                     /locus_tag="PLU15_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOO30346.1"
                     /translation="MQPNTTATALVILMYIASSIAGMRTNKTNPGVQTAPGIQTTSPT
                     PQTEKNAVLDLASRSAAISETANVRSGPGTNYPKTGKLAKNTPVRLVSQANGWYQVSL
                     PNGGYGWVAGSLLRIGQPSGAPAQLKHDVVGYYTVNYPGDKSSYNVLDSHSRSLTAIA
                     PFSYAVDRAGNVTGEHFHDAMEIASSRGLSNLALVHNLNGKSFNQSDIHSMLNSKAAR
                     ERAIRDIYDLVKKNGYDGVNIDFENVPPGDRRVLTQFMQELRSKLGPAGYKVTMSVPA
                     KYKDSPSSAWIGAFDYYALGKVCDQIMLMTYDQHTSGTGPGPVASVQWVEKVIKYAMT
                     QMPKKKILVGIAAYGYDWNTSTNRAKAISYSQAIRTAERHGTKISWDNASQSPYFRYK
                     SGNVTREVWFESADSMSAKLKLVKKHDLGGIAIWRLGQEDAGYWKVIERELQN"
     gene            25306..25770
                     /gene="dut"
                     /locus_tag="PLU15_05180"
     CDS             25306..25770
                     /gene="dut"
                     /locus_tag="PLU15_05180"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOO30347.1"
                     /translation="MPVIDERRPCPSLPGYMSGSAAGIDLMACLEDKVELAPGEYRVI
                     PTGLKMAIPEGYEGQVRPRSGLAAKFGITVLNSPGTIDADYRGEIGVILINHGFEPYT
                     IANGDRIAQLVIAPVVKVELLETAELDETERGEGGFGSTGVSDGDGKRCTDG"
     gene            25763..25960
                     /locus_tag="PLU15_05185"
     CDS             25763..25960
                     /locus_tag="PLU15_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30348.1"
                     /translation="MAEAIAARLEQLKTEIEELRAELRETATREGAIADLDRACSVSD
                     RLDALILDFLRVKDQVGHTVD"
     gene            26128..26613
                     /locus_tag="PLU15_05190"
     CDS             26128..26613
                     /locus_tag="PLU15_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30349.1"
                     /translation="MSKVIGTFASRDAAEFCIRELRNSGFDYNRISLVARESGKGGEQ
                     QGGGPTMAGGDTALEGTATGGAIGGMAGLLAGLGALAIPGIGPIVAAGPIAATLTGAV
                     GGGLVGGLVDMGIPSERGEFYEGKVKAGRILAVVDAGSDKVNDAARIMREYGASDVET
                     H"
     gene            26866..27657
                     /gene="dapB"
                     /locus_tag="PLU15_05195"
     CDS             26866..27657
                     /gene="dapB"
                     /locus_tag="PLU15_05195"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOO30350.1"
                     /translation="MNRVIKVVIAGAAGRMGREVVRAICRAEDMELVGAVDVVMAGQD
                     AGTAAGIGEVGIAICTDLAEVLDRTAPDVMVDFTVAKAAEANIKMAISRKVRFVVGTT
                     GMSASTMESIMNEAAQAGVGGAVVPNFALGAVLMMRFAREASRYFPDCEIVELHHDGK
                     VDFPSGTAKATAHEVASGRGDAVSRRGLDGGIRGVEVEGVPIHSVRLPGLVAHQEVIF
                     GGPGQTLTVRHDSLGRDSFMPGVLLAVRKVSQLDHVVLGLDPLLE"
     gene            27850..28455
                     /locus_tag="PLU15_05200"
     CDS             27850..28455
                     /locus_tag="PLU15_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HOO30351.1"
                     /translation="MALEGKTIGIALTGSHCTIPEVLPEIEKLIMGGAAVIPILSESV
                     AHTDTRFGRAQDIREKLEKMTGTRVIDTMTKAEPVGPKKLLDCLVIAPCTGNTVAKLA
                     LGITDSPVLMAAKAQLRNGRPVVIGIATNDGLSGCARNIGMLHSRKNVYFIPYGQDDP
                     IGKPTSIVSRMSEMERTVEEALAGRQVQPLLVEKWKDLRRS"
     gene            28575..29600
                     /locus_tag="PLU15_05205"
     CDS             28575..29600
                     /locus_tag="PLU15_05205"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOO30352.1"
                     /translation="MLRVAVVGATGAVGREIVSAIFELEFPVSDLILASSPRSAGSKV
                     STPMGEITVTNTEISALEGADVAFLAAGASVSRKLAPELARRGTLVIDNSSAFRMDPE
                     VPLVVPEVNIDAVHEHRGIIANPNCSTIIAVVAVAPIYRRWGLERMIVSTYQAVSGAG
                     HAAIEALADESEAVLAGLDPVPSVLPYKSAEVHHQIAFNVIPHVDSFCEDGYTREEYK
                     MTDEIRKILQDPSLKISATCVRVPVFRSHSESINLQTCQRAPMDEVRAVLASSPGIRL
                     MDDPVSFAYPMPIHVSGKDDVEVGRLREDPSAPNAMWLWVVGDQLRKGAASNAVQIGL
                     ALYDDRR"
     gene            29606..30823
                     /gene="dapG"
                     /locus_tag="PLU15_05210"
     CDS             29606..30823
                     /gene="dapG"
                     /locus_tag="PLU15_05210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO30353.1"
                     /translation="MGIIVQKFGGTSVADAQACALVVSRIAEARRAGNDVVAVVSAMG
                     RKPAPYSTDALLALVDCAGDTLPPRERDAIAACGEIISSVVLVSALVASGCPAMSFTG
                     AQAGIETDDNFGDARIVRIDPAGLHNAIASGVVPVVAGFQGVTDGGDVTTLGRGGSDT
                     TAAALAAALGADLVEIYTDVEGVMTADPHIVPNARFQETMTYSELIEMANLGAKVVHL
                     RAVEIASQARIPLAIKSVFSDCPGTLVTDIPPQRMPIAVPDRFVSSVTCIGNMAQIVL
                     EEYDFKADDAALNVFSTMADNHISVDMINVFPDMIAFIVSARDSARAVRALDRLGLSA
                     VANPGCAKVSVVGEGMRGVPGVMSRVVRALHGAGIRILQTSDSHYSISCLVPEVDMER
                     AARALHSEFELEK"
     gene            30856..31749
                     /gene="dapA"
                     /locus_tag="PLU15_05215"
     CDS             30856..31749
                     /gene="dapA"
                     /locus_tag="PLU15_05215"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOO30354.1"
                     /translation="MKKNGRRIRVITAMATPFAPDLSVDYRKARELARRLIENGSDGL
                     VVSGTTGESPTLTHEEKLELFRVVLEEVGGEAAVIAGTGGNDTRTSIELTQEAEKLGV
                     DGAMLVTPYYNKPPQAGLYRHFASVARSTSLPLMIYNVPGRTSVNLSADTMAKLANDF
                     SNIVAVKEASGSLEQAAEIRSKAPKGFTIYSGDDNMTIPMMSVGGDGVVSVASHVAGP
                     GIQQMVTAFIDGDVELAAEMHRRLLPLFKVIFITTNPIPIKAALKLAGFDAGGLRPPL
                     VEATDAELGQIEAVMESLGLI"
BASE COUNT         7024 a         7826 c         9738 g         7473 t
ORIGIN      
        1 acgaacgatt ctgtcagagg tgtgatacca ggctgatcat ctggcgccat cctacctgat
       61 ttgggtggtg tcgtttctgc gagcgaggat gcttgccccg ataattgctc gtgcctctgg
      121 cacatgcggg atggcaggct ctggcagggg agggtagagg attatcgaat accgctgcta
      181 tcatggagaa agaatgtata caggatttgt tggcagggtg ggaaagagtt gctttcaaga
      241 cttgcaagga agatcatgcg agcgatagca ggtgacaggc agcggggcaa gaacatcatt
      301 tctagcgaag gcgattcggc tcaactcagc tccgacctgg agaacaacat cgcctacatc
      361 gtaaaggcct tcggcagcag cagcgacctt gttgtacgcc ggcttcgagc aggaaaaggg
      421 ggctcggtgt cggccgctgt cattcatctc gatggtctag tggacgggct tcttgtggcc
      481 caaagcgtag ttcgttctat tacggtgatg acagagagcc tcgattcgcc cgagagtgtt
      541 ttggacgagc ttcacgatgg tctgatatcg gtgacaggcg tcgacaaggt cgtggatgga
      601 tatgaggcga cgttgcggat tgctgaaggc tcatgcgtag tgttgataga ctccctgcca
      661 actgcgctcg cctgcgccgt gcaagggtgg gagcagcgca gccccgaaga gcctaacaca
      721 gaggcgacgg tgcgtggttc gaaggaaggc ttcgtagagt ctatacgggt taatacctcg
      781 atactgagga gacgaatcgc ccatccccgt ctgcgtatcg aagaatgccg gctcggcagg
      841 ctgactcgga cgtcagtggt aatcgcttac gttcagggaa tagctcgtcc cagcatcatt
      901 gaagaggtga gatcgaggct agcggcgatc gatatcgact cgattcagga atccgggcaa
      961 ttggaggaac tcatagaaga tgctccgttt tctccctttc caaccatagc ccgaaccgag
     1021 cgacctgaca gaattgttgg agctctcctt gaggggaggg tggccatcct tcttgacggg
     1081 accccatttg cgctgatcgt tcctgttacc ctcacgatgc ttctgtcaac tcctgaagac
     1141 tatttcgaac ggttttacgc tggaacactg atcaggttga tcagagtgct atctgtcttc
     1201 gtctcgcttc ttatgccctc gttctatgtt gcagtaacaa cctatcatca ggagatgctt
     1261 ccgacttctt tgatccttag cattgccgct caaagagaag gcgttccttt tcccgccttc
     1321 cttgaagcct tcataatgga ggtcatgttc gagctgataa gggaggctgg catacgcctt
     1381 cctcgtgtca ttggcccggc catcagcata gtgggggtgt tggtgttggg tgacgctgcc
     1441 attcgagcag gtctcgtatc gccgatcatg gtaatggtcg ttgcagcaac tggcattgca
     1501 tctctcgcaa cacccgcatt tagccttgct ataggaatca gggtgcttcg tttcgccttc
     1561 ctctttctcg cggcttttct cggctttttc ggaatagcca taggcgcgct tgccctcttc
     1621 gcttacctca catccttgga gtctttcggg gttccctaca tgtcgcccat cgcgccggta
     1681 attgacgcag gacttaggga cgcgattttg cgggtcccgt ggcgggcgat gaaggagcgt
     1741 ccggaatggc tgacttggta caaccagacg aggatggatt cggcggcaaa ccacaaggac
     1801 gaacatagca cgtttcggga aggccgcagg aaggagcaat gactatgtcc cataagctga
     1861 ccgcgcgcca gttagggtcg attcttttct tacagagggc tttgcctgtc actctgacgt
     1921 tcccaatgat gataggcatg cagtcttccc atgggttgtg gatagcaggg gccttgggta
     1981 ctctcatctc cctgcctctg ctggtgtgga tggcgtcgct gtcaagggcg gaggggcttg
     2041 atgacattat tgaaatatcc cgccgccgtc ttgggacgac tgtaggtgga gcagtaggct
     2101 ggctcttcgt cttctattgg atgcttcttg cggctttgca agtgcgctcg gtgggagagg
     2161 cttatgtgat tggtaccatg ccggagactc ctatcgtagt cttcatggtt ctgacggcgc
     2221 tggtcagttc gggcatagct cggcgcggca taaagttgat tgctatgatg tcggagctta
     2281 cagccgtttt gatccttctg ggattgctgc ttacgttcac tcttcctgca gacgtcatgc
     2341 aatttagaaa cctgttgccg cttcttcctg aagacctgag ttcattggcg ctcccgactg
     2401 gaactgcagt cagtctcttc ctcgacctta acgtgctcat gatgatagcg ccgtacatga
     2461 aaagcggccg ggatcttatg agaggcacag tctattctgc cctgataagc ggcgctatcc
     2521 tgattcttct tgcagtggtt gttacagcgg tcttcgggcc tcttgccacg tcacttgagc
     2581 tgcctgccct cagccttacc cgcatgatca gccttgggga attctttgaa aggctggagc
     2641 tgattactgt tgcctcatgg acctccgggg cgggccttgt gctttccacg tcgctttggg
     2701 cggcggctga ggctagtgcg aatttgcttg gcttaaagcg ctatgaacca ctagtctacc
     2761 ccctgggagg gttggcggtg atcatgggat tgggtatgtg gcccaacatg ggagcatttg
     2821 acaggtccgc ttctgccaag agcgggagcc ttgtcacggc ggtcttcatt attgctgtgc
     2881 tggttgtgct gactggagca aggtggttga atcgacggaa aggcgagggg cccggcggga
     2941 cgagaatgat agcggcaata ctagctctag ggcttactgc atttcttgct acagggtgtt
     3001 ggagtcacag ggagattgag agcctgggat ttgtcaacgc agtgggagta gataccgcat
     3061 tgggcaagac ccactgggaa ttgccgggtg aagaacgcga tcccggcgag ttgattcagg
     3121 tcacggccca cgtagtcaaa ccttccgcta tagtcagtgg cgaacgtggg cctgcacctg
     3181 agaagccgtt ttgggtaata agcgcgacag gatataccat ctttgaggca gtgcgcaatg
     3241 tgtcggagct ttcgccgcgc aggctctcct ggccgcacag tagatgggtg ctattcggcg
     3301 aggagttcgc aaagggcgga gtggcaaggg cggttgactt tttagtgcgc gaccaagaaa
     3361 ctcggcggcg agccgttttg ggcgtggcct caggcgctag ggcatgggat ctgctccaat
     3421 cggaatttga gttggagcga gtgccagggg aagcggggat gggcattgcg atgaacgctt
     3481 cgaagagcac gtcgactatt gttattgcct cagtcaatga cttcgttatg gcgttggaat
     3541 cggaaggaat tgatccaata gctttgcgca tagaggtcat gccgtatacg tatccttacg
     3601 agatcacggg agacgtcaca cgggagcaga ttaaatctgt ggctaggttg acgggcgcag
     3661 cagtatttcg tagcgataag ctggtaggat ggctcgatgg acgcgaagcc agaggataca
     3721 attggataac gggaaagacc aagtccggga ttctggtaat agatgcgcct gaaccgagct
     3781 tgggtcgggc ttcgttaggg tctcgcgttg gacttgaaat catcagatct agtggatcct
     3841 tcagacccaa aatcgaagac aatggaagaa caatagggat cgtcattaag ataaaggcag
     3901 aggctaacat atcggatgtg cagccatacg tcgacctgta cgctcaccct ggactgtggg
     3961 agtcgatgga gcggctgatg gcaaaggcga tagaggatga aatcatggcc gctgtgaaga
     4021 aggctcagga tcttcgagca gatgtattcg gttttgggcg cgagatccat cgaacccgtc
     4081 cgaaactttg gaaagagatc cggaacggtt ggtacgacat tttcgctgaa atagagccgg
     4141 agatcgagat cgaggcaacg ctggtcagga gcgggctgac tgtgcgcagc gtcaagctga
     4201 atgaaatggg aggatcgggt ggacagcagt gaatatagtg ctgctagtct tggcggtcgt
     4261 ggttttggga ttctatgagg cgccggacct cataaggaac aagctctggc gggagctggc
     4321 cgtgtttgtt ttcgctgtag ggctcgactt cacgctggcg cttttgatgt tactcggggt
     4381 gaatattccg aatccggcga tctggataga caaggccgca aagtggatgc tttctctctt
     4441 ccgttaggga gctgtcggtt gcaggccaag cacggtcagt gttttgagag gccaaacagc
     4501 cggtctgtca cgattttcca tgcgttccag ccaaagacgc attcaagttg tttgtctcat
     4561 gcctatcgga tctatgtgcc aggcgaattt tggtataggc ctgtaaaaac tcttaggcac
     4621 gcctggcaag ctctatatga ggggagaaga ggaatacggt cgcaggtgga gaatgacgca
     4681 gcgagcgcgc aggctcataa atcgtctcca gaacctgtat gatgcgcgcc ttgccgcgca
     4741 gaaaatcgac tcggtctcag agtggcagag cacattaatg gggtcaatga tcaagtggag
     4801 aagttgagaa tgttttctga gtgcacccga ccggtgctcc gcattcaacc ttgggatgcg
     4861 ctattgcctc atgtgccgac tgttttagag ctgagatccc tgccttttgg gacaagtctt
     4921 tcgagcacga aggccctgac aagcttggct aggtcattga tgggggcaca taaggcacct
     4981 gcgctggcgt ttactgcaat tcaggcggtt tcgcctatgc tgttcgttag atcgaacacg
     5041 tggaaagccg tacccgaagg aatgatcggg ctttacgtca cgacaggggc gtccttgcat
     5101 tggatgtggg attatcctct cgggacccga atgccggcga catgatattc ccatgctggt
     5161 acagaaactt tccaagttta ttcggagaag tgcggacgtg aggtgtgagc cacataaagc
     5221 aggctaccca aaggcctctt tctgcaagca gctgcagacg atatctgctc taagcatgac
     5281 cggtatgccg tatgtgctcc atctagccac tgaactctgc catattctat gaaacacgtt
     5341 tgcccgatag agtttcccga atcggtgcga gaacagcccg ttctcgcgta ggtttgatat
     5401 tgtcagcgtt gggcaagaat gtgggcggat gctccagttg aagctgtaca aggggagatg
     5461 gtcggatatc atggtgggca gagctctgct ctttgcagga ttggtggcgg ttgcggggta
     5521 cgtggtggtc actgtggtcg gagcggagat gatcccgggc tataatcacc tttcacaccc
     5581 gctaagcgag atgactgccc agggcagcgc aaccaaacct gctctcgatc gatggttcat
     5641 cctgctcgac acgatggtga tcggatttgc atctgtgatg ccagcctccg tctgtactca
     5701 aaacgacggg gtcgcaacgt atgtgcagcg cggagcggca ttccttgccc ttgcgggcgt
     5761 tttaggtata atcatgacac tgtggtttcc catggatggt tcgattctcg ttactactgt
     5821 cgtcggaacc cttcatgagt ttatagccgc cgccataatg gtgtgctcga tgttggcgat
     5881 cctcctcttt ggattcggtt tgcgaaacag ttctgaatgg ggtggctttg cattctactc
     5941 cttcgctacc ttcgcttttg cgggatcatg ttcgattgtc accgcgcacg cctttctcct
     6001 tggaactgat ttgcaggggg catggcagcg tctgaccgcc ggcgcgattc tgcagtggat
     6061 atttgtggta tctctcaggg cgttgtctgc gccaatggac tgacattggt gactttcttt
     6121 gccagagcgc gattttgagg ggaatccatc cataggccat tagcaggtga ctgagaccga
     6181 attgagatga tgattgctga tatccgtctt tcagcaaact tgcgcaacga aacgtacagc
     6241 cgtaatgtgc agatatatcg aaagaagggt tgatattctg ttaacgatcc gtcaagcgtg
     6301 agggcttaac gttgcattga gtggcagagg ctcgagttga tgccatcccg gtttggaatg
     6361 gctaggatca catgctcatg acctgaggct acgtgtgtaa gtgaaaagct ggcgcgagtc
     6421 tcaaagtttt gcatacacag attctttttt gtgaaagcag gttccctgag gcgcgcccga
     6481 cacatgatag atacactttt gccagaaacc tgtatggaaa agatgcaccc gtctgacatt
     6541 gaagaccctg gtgcaatcct cgagccggtg gtgggcattg ctgtctgaat tgcagcctcc
     6601 atcgagacaa gtccgataat cacggagatc ggaaccttgt tcctgctttg tatgacgacg
     6661 acgggggcga ttgcagcttc ccggagggcc atgtaaactt accttggaag ctccgctgtg
     6721 ggctccattt tgactgaagc tgaggtgttg caagatgggg aacgagaagg atcgcaagac
     6781 tggtttgtct gtcgaagcgc ttcttgcagt cttcatagca ttggcctgta tactaactgt
     6841 tttcgcgatt gcgaatcaga gacgctcttc cagggtggag atttctgatc ttccggagag
     6901 ggttcgcttt gcagtgcttt gcactatgga tgcgaatgca ggcggcacga agagcgtggc
     6961 ttgggcgcct gatagccagc gaattgtcac tggcgaaggc gacgggcttg tggtcgtgtg
     7021 gaatgcccgc tgtggcgaga agagactgac gctccacgga aggcccggct accagatcga
     7081 aaatgtatgc tggagccctg acgggaagca tatcgcggct attactggcg aagacgagtt
     7141 cacagtctgg gattcaggga gcggagaagt tgagtggacc gttgttgacc ctgaggtgta
     7201 tggactggaa ggctggagcg aggacggcaa gtcggtgacg acctggaacg gggaggaata
     7261 cttctcttcg tggaatatcg agtcaaaaac catggagaat cgttggtttg tcagcatagg
     7321 cgagcagagc agtgtgccca gccccgacgg gaaactcgct gcatggcagg tgggtgagga
     7381 tgtctgggct atccataccg tttcgtctga aaagttcgat acaaaggaac tttgtgggca
     7441 ttcaggtttc gtgcgtcatg tctcttggag tcctgacagt tcccgtctgg ccggtgccac
     7501 agatgagggc atcgtggtat ggaacgtcga aacgggtcaa ctggagaagg aacataagag
     7561 agctgccggc gggtttgcca atgtgttgtg gagcccggat gggcgccaca tactggccta
     7621 caccgatgac ggttccggag taatattcga cgctgagcaa gtcgatccgg ttgcagatat
     7681 cagtgttgat ggaaggcgtt ggtggtcttt acctgtggct tggagtcctg acgggtctct
     7741 ccttgcagca ataacccagg accaaacggt gttgatatat gggcagataa cttactgatt
     7801 ggccttgcca tattaatagg gattcgctct gggccctgtg ctttttccag agcccggcgg
     7861 ggtagatagt cttcgaggcc tcgtagggct ctggaaagag catgcagcca ttgaggctgc
     7921 aaggaaacga agtgtgggaa ctacgagatc agcgtcttct aaacgcaggg acttaggctt
     7981 gccgtgaggt tcttcgtgcg cactgttgag agggatgttt aacttccgcc atggctgtgt
     8041 cagtgtttgg acttgtcatg tgtataggcg tcccatgcct cgcctagttc gagccttggc
     8101 gatagctgat ggaaaggcgt gaagaaaacg ccgagggcgc cgaacgccgc agtcatagct
     8161 gttacagctg ttgccgcggc tatcatgagc atcaccacga tctggtatcg gactgcgtcg
     8221 agcgggcttg tgcccgaaat catctgtcct gtcatcattc ctggaagatg caccaagccg
     8281 acagtcatca tggagttgat tgtcggcatc attgcggccc gcaacgattc ccgcattgcc
     8341 tcggatgccg cttgtttggc gggagcgccc agcgccagtg cggcctcgac ttggtttcgc
     8401 ctgagggtaa gctccgagtg gagtctattt acaactaggg ctgctccgtt catgcaatta
     8461 cctagtatca ttccagctat aggtatcacg tattgcggca tgtaccaagg gcgcacccga
     8521 aggactacgc ctattacgag aatcaaggta attgatgctc ccgttgcgat ggcggccatc
     8581 atcactgtga acaatccttg cttggcgccg gtctgacgcc ttactccgtt gtgtgctgcc
     8641 actgtagtca taatggcaag ggcaagcaca acccaatacc aacgggctgc gtcaaagagt
     8701 tgctgcagta cgaacccgac agcaagtaat tgcgcgaagg ctctgatggt tcctacagcc
     8761 atgtcgcgtt caagctccag cttctgccag cgggaaattg ctccggagat agcgatgaag
     8821 agaagtgcaa tgagtagatc catgttggaa atgtgaatat acgagttagc gtccatcctc
     8881 tgtcagctct cctttgagaa atctgtggcc aatttgagtt ttggggcctt caaaaaaatc
     8941 cgcggctgcg ccttcttcta tcaggacacc gccaacaagc atcgccacgc gcgtcgctat
     9001 ggcctgggcg tgctccatca cgtgggttac gatgatgact gtgagcccca gttgacggtt
     9061 gaggttgcag atgagttcta ttatgttccg cgctgcagtg cggtccagcg ctgacgtagg
     9121 ctcatcaagg agcaggatgt tgggacgatt ggccagagcc cgcgcgatac acatccgttg
     9181 ctgttgtcct acagacagag acgaagcagg ccttgacacg agttctggct ccaggcctac
     9241 aagctccaag tattcttcgg ggttgcacgg ttcgcctctg agactggggc cgtacactat
     9301 gttgtcggac accttcccag gcagcaaggc aggggtttgc gacaccattc caactcgacg
     9361 tctgagctcc ctcacatcta atcctgatat gtcggaccca aaaagtaaga tctggccgga
     9421 agtagggtct tccattctgt tgatcattcg catcagggtc gatttccccg accctgaggg
     9481 gcctatgagg gccaggattt ctccctcggg cacagagaac gagacatcac gtatgatgtc
     9541 aagctcggtc aacgagttgc ccaagaggac gggctttgtc atgcttacgc ggttgagctc
     9601 aattgcaggt acgcaagtca ttgaatccat tcactttgtg gcctccgttt tcgatcgttt
     9661 acaagctttg tcactttaac agggaacgtc tgtatgcatt ggaagttgcg gcggaaggac
     9721 acgaatttac caaacgatta cgccgctctg cttccgaacc gtgctgtgcg gttgcaagcc
     9781 atttgtaaca cagcagtaga caggcagggc tttattggcg gccaggcctg cctcttagat
     9841 tctacaccat gttgtgctta agtgcgcgac cttcgaaatg accacaagtt tggacacgag
     9901 ccttgcaggt atgaccttgt tgtccactcc cggggcgcct gtgaagaatc gtattaccat
     9961 cacctcgccc agagtcaagg agaggccatg cttcgagaaa atccacatgg aaccgatcca
    10021 gctggagagt ccaccccttt caaactatgt gatcagctag tcgagaagga aggcgaggag
    10081 tgctgcaacg gtatggtgaa cacggctgcc tatattactc agccatatgc tcttcaacag
    10141 ttgcggcccc ccgatgccgc atggaatccg cggcccgagg caggttttgc tcggaaagtg
    10201 agaaaaggtt gatggagtat gtccgtttga gatgtgcgcc cttcgtgctg gagacggctc
    10261 ctgtacctct atttccataa tgaggagcaa tgataacata accttgctat gagaagaaac
    10321 ctattggact tgtggcaaga cgacagagac gatgagactg cggttccact atacagcaca
    10381 agctcacgct gctggacgat tagtgcttct taaactaaga aaatgcgctc atgctgtctg
    10441 ggcttttgcc gaatgggcat accgggcggt gtgaagctgt ttcagacgcg catacaggcc
    10501 ggactgggca tgatggtcca tcgaaataga gtgcttgagc aagcgctgaa ggctgaccgc
    10561 atctatcgac ggtgtcctat gcaatccgcg aaagcgtctg ttcggatcgg cgcttcgggg
    10621 cactatccgc aaagaacatg agggtgcggt gagggcatgt ttgagcagca ttagcggcaa
    10681 aggcgcggga acgagagtat gggcagaaca aagtagcagt gatcgaagaa aaagtggggg
    10741 tagattgttg acagagcgcc tgggtagctt gcggggatag ggcgatgcaa cctgcttgaa
    10801 tcgggtgata gaatgaattg tagttgatac ggaggaagga ggaccttctg tgcgtgcgct
    10861 cgttttcaag gatggactta gagaagtgga tatgccgata ccagagataa ctatggggca
    10921 ggccttggtc cgcgtgacac ttgcgggaat ctgcaacacg gacatagagg ttacgcgtgg
    10981 ctattttgcc tacacgggga tcatggggca tgagttcgtg ggcgtggtag aggatgccga
    11041 tgatcccgac ctcattggta agcgtgtggt gggagagata aatgtgagct gcggcgagtg
    11101 cccgacatgc cgtggcggca acccgcgcca ttgcgagcat gtaaaagcca tgggcataaa
    11161 cggttggccg ggttgcttcg ctgactatgt ggtgctgccc gaatgcaacc tccatgtcgt
    11221 cccggactcg gtctcagacc atcaggcggt ttttgtggag ccattggctg caggggttga
    11281 agtgatggag caggttcaca tcaggccgag cgacgaagtt gtggtgatcg gtgatggcaa
    11341 gctgggcctc ctcacggccc aggccataca tgcctgcgga aaccgcgtta ctgtgttggg
    11401 aaagcacgtg gagaaactgc agattgcaag ggatctcggg atggatgtat cactcgtagc
    11461 cgacttcgtc gatactgtcg atgtcgtagt cgattgcacg gggaatccat ctgggatgga
    11521 aactgctatg gccatggtcc gtcccaaggg gaccatcgta cttaagagca ctttcaagca
    11581 gggggccccg ctgaacctta caccgatagt ccgcaacgag gtcacttgcg ttggttcgcg
    11641 atgcggacca tttccgccag cgctgaggtt gcttgagacg ggggcgatcg ttactgagcc
    11701 gttgatcgag gcgatctacc cggctcatca gatggaggca gcgtttgaaa gagcatgcac
    11761 ccggggaagc ctgaaggttt tgatcgattt cacgtgctct caaggctcag gagaagttgg
    11821 ggcggggaag atggagcgac tgcatttcta gtcaggttcg catcgtgtga tcatgcgcat
    11881 ttcagggagt ttctgacggc tgttacgttt tcacagagcc ggtacaggag ctccctaatc
    11941 tctttggcaa gggctttatg ctcttcctaa atggtatgcc catcatcaag gcttccgtaa
    12001 gcttttgcca agaggcagca tacttgctca aacaggggat tcatctgtag caatgttcca
    12061 ccgttttgca gcaaaaagtt aggttcctaa gccatgaagg aacggtttgc cgagaagttg
    12121 agggcaaagc tgtaattcct gtgaaggctt acgtcccgga ttgcgaaagg cggttgccca
    12181 tttcgtcgcg tctttggacg ttacagcatc ggtaccgaca aaggatttgc cagatcgata
    12241 tagaaaccta gcatgtagtg gcacatatgg gctttcgcgc caagtgcgga ggtcggaaat
    12301 ggcggtgcac agaatccggt attaaaagga gagatgggga tgagaaaact atatggcgtt
    12361 ctcacagtct gcacgctggt ggcaatggcc ttatggctgg cgccagcgtc agtgggctca
    12421 ggcgaacccc tctacgttgc gttgatatgg cataaccacc agccattcta caaaaacact
    12481 gcaaccggta tgtatatgat gccttgggtg cgcatgcatg cagtaaaaga ctactacgac
    12541 atggcatcga tactagagga ttatcccaac gttcatgtca ccttcaacct ggtgccgtca
    12601 ctcatccttc aactagatga gtacgcggac ggggctcaag acgtgcagct cctgctttct
    12661 ctcaaacctg catcggaatt gaccgatgat gacaaagact tcattctgag aaggtttttc
    12721 gatgccaact gggacaacat catcaaaaag tacccccgat attggcaact tctgaacaag
    12781 cggggacagg gagtgaccga tgaatccatt gccgcggcta tagcggccta cgacgaacag
    12841 gactttcgcg acctgcaggt ctggttcaac ttggcctggc tcgaccctga cttcattgat
    12901 gaagatccga gtatcgaggc gttggtcaga aagggccgag gcttcaccga agaggataag
    12961 cgagttgttc tcgacaagca taacgagata attcgcaacg tcattccgat ttatcgggag
    13021 atgcagaacg caggacagat cgaggttacc accactccat tctaccatcc gataatgccg
    13081 ttgatctatg atgtcgacct tgctcgggtc gctagcccca agctggagct gccgagtgaa
    13141 ccgttcgctt accctgaaga tgtcgaggcg cagctttcga aggccgtcga tttctacaaa
    13201 gatcactttg ctgtggctcc acgcggactc tggccgtcgg aacaggcagt tgggcaggac
    13261 atagtcggca tcgtgcacga ggcgggtttc cagtggatgg tttcgagtga aggcgtgctc
    13321 gcgcaatccc taggaatagg tttgcgggac ggggcaggca ggatcgtccg gcctgatgtc
    13381 ctctataaac cttacatcgt cgagcaagac ggcaaagaag tcacgatact cttcagagac
    13441 atcgttcttt ccgacaagat aggattctca tattcaggga tgagcggtcg cgcagccgct
    13501 agcgatctca cggactatct gcgaagcgta aagaggagcc ttgcctcaac tcccggcccg
    13561 catgtagtca cgatcgccct cgatggcgag aattgctggg aatactatga aaacgacggc
    13621 aaggagttcc tgcacacgct atacgaagcc ttgaatgatg atccggattt caaggcagtc
    13681 acagttgagg aatatcttgc cgcgcatccc gcaacagaga gaattcccat gttgcatact
    13741 ggctcatgga tatctgacga ccttgagaca tggataggcg aggtggaaga gaatcgtgca
    13801 tgggattatc tgtcgaaggc ccggacggct cttgccggct tcacagcaga acacgccgat
    13861 tcggctgatg atgagatgat gaccgccctt gaacgggcat gggaggagct ctacgcggct
    13921 gagggctctg actggttctg gtggtacggt gatgaccagg attcgggcaa tgatgaagcc
    13981 ttcgacgagc tcttcaggat ccacctacaa aacatctact cctacatcgg ccttgaggtt
    14041 ccaaactatc tttacatgcc cataatcccg aagcgtgctg cagtcccgga agagcggatt
    14101 accgggctca tgcatgaacc cgtgatggac gggttcatcg attggggcga atgggagaag
    14161 tcgtcgtttt atcccaaaga cgatgctgct gtgttcagtt ggggtgaagg cgagaatatc
    14221 ttctcgtctc tgcgtgttgg agtggataac gagaacctct atatccgcat aagaacgcaa
    14281 gacggatttg atgagcttta cggcaaagac attaagatcg ctgcatatct caccaatcca
    14341 aggcggcacg aggtcaacac tattccccgc gggagcgcat caggacgcga tgccacggta
    14401 ttgggattcg ctccgggaac ggagatacta gtggactttg caggtatcga aagagctggc
    14461 cgcgcgaccg cgctcctgtc ggtggcggca ggcgacgata cctggacaga agtgagtcgt
    14521 attaacacag ttggagtggg ggaggccctt gagatctccg tgccatttga ggttttgcgg
    14581 atgaaaccgg ccgatgtagc tcacatcgtt gttgtagcat ctagtaagga tcaaaacatc
    14641 gacatgatcc ctacatcggg gccggcagag ttcaaggccc cggtggtcac cgttgggcgt
    14701 cggctcttta gggtcgaaga tccgcgcggt gatgatcatg gtcccggaac ctacagctat
    14761 cccaccagcc ccgtgttcac gccgggagcc ttcgatatgg tttcgtgtga agtcatcgat
    14821 tccggtgagg aggtcgtctt caacgttgag cttgcaggag agataacaaa tccttggaac
    14881 tccggaatcg ggctttccgt gcagactatc gatatctaca tcgatacaga caacaaggta
    14941 agctcgggat cgactacagc gttgggtggc agaagagtcg agtttgaacc ggaatgtgcg
    15001 tgggaatacg cgatctgggt cgaaggatgg aaccaaaggg tcttcatgcc cgagggtggc
    15061 gatgttggtg gcgtcactgc caccgttgac accataggca atctggtttc gatttcggtt
    15121 cctaaatcca ttataggcca gccagagccc ggttggggct accaagtgtt cgtgcttggg
    15181 caggaaggtt atccatctga gggcaatctg cgtgtccgcg aggtgatgga acaggccgca
    15241 gagtggcgtt tcggtggagg cgacaacagc gtgtacgacc cgaacgttat cgatatgctt
    15301 gttcccacag gacgtactca agaggagata ctttccgcct atgatgtttc cgccaagaag
    15361 ctcgcagtgg taccaatgat ataccctgac tgggaatact agctataggg ggccgcgtgg
    15421 cggccccctg tgtatttgcg ctcgaggcaa atgccgacag atttctgata tcaaccgaaa
    15481 ggacgggttg cacagtgtca gagagagtat ctttttggtc tgcaattatc gtggtcttgg
    15541 cattagcgtc tcccgctttt gcggcggaga ggattcctgt tgcattttca ttcactcctc
    15601 cgatggcagc aaacacagtg gcggtggcgg gcacattcaa tgattggaac cctactgcaa
    15661 atctcatgat aggccccgac gaagatggaa tttggaccgc cgtaatccat ctacagcctg
    15721 gacgttacga gtacaagttt gtagttgatg gtaagacgtg gcatcatgat cccaatgcgg
    15781 tggagcaggt tgcggaccca tacggcggca agaactccgt agtctacgtc ggagtcgttc
    15841 ccgtggccga atcttccatt gcttttgacg ggtggttcga gtcgaagctg gcgaaggagg
    15901 caggagagcc tctttcgctc tcaggcgacg cttacctcaa gttcagcgga gaactccgcc
    15961 cgtgccttga aacctatact gagctccatg catggaagtc cctctcgcta ggaggcgacg
    16021 caggttggca tttgatgccc cccattgacg gtcttgaagt gaaccagtct gtggccactt
    16081 tccgcctctc cgacgggatt gcggccaagc ttttctacag agggcatgca ggaaacacta
    16141 aggatcctct cacccttata gcttcagaag ggtcagacaa caggatatgg gaccgtcgtg
    16201 cagccgagat tctctgtttt aaggggattg ttgacggaag gataggcgcc gcaacccttg
    16261 ccggcaacaa gacgttggtt tatggggaga tggaagccgg cctgacggat agaatcacat
    16321 tgggaggagt ggctacatat gagagatggg ccacgagtgc tagggaggat ggggaagacc
    16381 acagggccaa catcggagct tacgcagttg caaagcccac agaagccttg accgttcgcg
    16441 gtcagttgct ccagacccac ggcactaaga tagtatcaga tagcacagtc cctgtggaag
    16501 tcgagttcgt ctacccggca gaacgcgaat cgtcaggagc tgacgtgatc tacatagtcg
    16561 gttcatttaa taactggagt acggaagatg gcttgcccct tgccaaagat gctgatggca
    16621 attggagggg ctcaatcgaa ctactcgaag gggaatacga gtacaagttc tggtaccctg
    16681 accctgacaa cgatccgaat tggggccact ggatgggaca ggggacgggc ggagataatc
    16741 tggtaaggca tgtcggcggg gacggcccgt cctttcagga tttctacttt gtggagcctg
    16801 ccgagcatca agtatacatc cggggatcct gggattggag caaggaccat gagctcgtga
    16861 gggaccctct tacgggctac tggaaacata ccatgaggct cgatcccggg agatacgagt
    16921 acgttttcca ctataacgat ggaagccagg accattggtt cggacagggc ggcgacaacg
    16981 tgcgctttgt ggccggagca ggcgggcgtg aggcagacta ttctgcgctt gcttattttg
    17041 cggaagcaag ctatcaggcg gacagatatg gagttacttt gggccttaag ggtgcacaag
    17101 acggcgttgt agcatcctac gggcacgtag gcgcagacta cagaaccgtg tatggaaagg
    17161 cctgggcaca ggcggcagag aagataaaat tgacactgga tacttcatac tccacagatg
    17221 ccgcgggaac caaggattcg gcgactttga cggtcaggcc cggcttggag attctagagc
    17281 cggctcaaca tgtggccttc ctgaagggat catacgaata caggaaaggg aagagcgctg
    17341 gcggtgtcgc gtcgctttcc actaggatag gttctgcggg tcttgcagca gaattcgccc
    17401 atgcccctct gggacccagc agcctcaagt tgagcgtagc caatgatttc ccatacgact
    17461 tatcagcgaa agcggagtac tggaagaagc tgaagacaga cgattacagc ctgaccatag
    17521 aagcgaccaa gaagatcgat tggtgtgacg gaaccactat aggtgtatct tgcgccttgc
    17581 caggcaggaa atggggggcc aagttgagtg tagacttcta ggttagcgca tctaagatag
    17641 tggcaggaag ggtccgtgcc ccataaaggc gcggaccttt gcgttgtccg ctcatgggcg
    17701 gtgtcccact gcacggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct
    17821 atggaggcgg ttgtaagcgc gaggccctaa tgtgtagaat agagacaggc ttaccgaagg
    17881 gagataatat aatggcccgc ttcggcattc atctttctat ttccggcggt ctgaccaatg
    17941 caacttcgag ggcagtggag ctgggttgcg attgcttcca gatcttcagc cgcaatcctc
    18001 gtactttgaa ggcgaaacct ctcgatcccg gcgaggccaa ctcatttcgc gcggctgttg
    18061 tcgaggccgg actgaagccg gtcgtgattc acgccaacta cttgatcaac gtggcaagtc
    18121 cgaaggatga aacgtatgaa aaatctgtct cagccctcac cgatgagctg cgcagggcgg
    18181 agattttggg cgctgattgc cttgtcctcc atcccggcaa tcacctgggt tctggagtgg
    18241 aggcggggtc tcggcgaatc gcatcggcta tcgatcgcgc ctacgaagat tcaggcgcgt
    18301 tggtccctat ctgtcttgag aacatggcgg gcgccggcac tgagataggc gtctccttta
    18361 gcgagctccg ttccattatc gacatttcgg agaggggccc atttctcagg atctgcctgg
    18421 acacctgcca tgcttacggg ggaggataca atgtcgcaac tgagcggggg cttgacgcaa
    18481 ccttaggcga gctggactcc gctgttggat tggatagggt gatgattgtc cacgcaaatg
    18541 attccaaggg tcagttggga tctggcaagg ataggcacga tcacatagga gaggggtcca
    18601 tcgggctggc tgggtttcgc gccatccttg cgcgtccgga gcttcgaagg ctcccattcg
    18661 tgctcgaaac gcctgttgat gctcctgagg accaggttcg ggacttagct gctctaaagg
    18721 cgtgtgctgc gggggaagag cgttgaagag tggagcactt gtcggggcgg tctcaaacta
    18781 catcgcttcg gaggccgcgg aaagtctgag gcaagccata gcggaggccg gcggaaccga
    18841 agtctttgcg gtggggcgtt gtgaccgcga ttttatcgtc actgagatat ctgtgttggc
    18901 gcgcggaaac ctgactgcag cgcctgttgt tgacgcaact ctcatgcacg ggcaagtagt
    18961 gatacacaat catccgtctg ggaagctctt gccgtccgaa gctgatttgg acgttgccgc
    19021 aagactggcc gcccggggcg taggcttctg gattgtaaac aacgacgtct cccagatacg
    19081 ctcggttact gatcctctgg cgaatcaggc gggctcgatc gtgattgacc ccgccgatat
    19141 caggcacatc ttctcgctag acggcccgtt gtcggctgcg tttgaggatt acgaggtgcg
    19201 ccaaggccag atagacatgg cgctcgcggt ttcgtcagca ttctccgact ccgcctgcct
    19261 tgttgtcgaa gccggtactg gaaccggcaa gtcgttggca tacctcgttc ccgcatttct
    19321 ttgggcgaag cggaacaacg cccgcgtcgt tgtatctacg aatacgatca accttcaaga
    19381 gcaattgata cagaaggata tacctgcgct tgaggcggct atcggagagc ctctccccgc
    19441 tgcgctcgtg aagggacgcg gcaactattt gtgcatgaga aagctcaatc gcgtgctaga
    19501 ggacgaagag tcttcgatcg aacctgaata ccgcgacgga tttgcccagg ttctccaatg
    19561 ggcgatggag acggagactg gggatagatc ggatatgcca ttcgagcctg actccgaggt
    19621 gtggggcatg gtatgttctg aaggtgatat gtgccttcga acgaagtgcc caaactacgg
    19681 ccaatgctgt ttccacaggg caaggcaggc aatggaaaaa gcggaggtgt tggtggtaaa
    19741 tcaccatatc ctctttgcag acgcgtttat cagaagccag atgggtcccg aagctgatcg
    19801 ggcggttctg ccccgttaca acgtggtgat cgtcgatgaa gcccacaatg ctgcggacgt
    19861 cgccacacag catttcggca ggcaggtatc gcgggtttcc cttgtgcgcc ttaccaacag
    19921 catatatcgt cgtgaacgcg ctgcagcgca gtctgctggc acggcctcca caggtgatta
    19981 cggcgcgctt gtgaggcttc gttcgagcct gtattcttca tggcccgggg agtcaggccg
    20041 tgcgcgagcc gaggcttgcc tcgacttgat ccacatcgac gcaattcccg ctgtcattcg
    20101 agctcgagag gcgggggagg ctttcttcga tgaagtccgc cgcatgctca tggatgtgcc
    20161 gggggacggc gttgagcgga cactccggat caccggggat gtgaagcaac gcgaggattg
    20221 ggacgccaga atctttcctg cccacgaacg ttttgccgca gctttggagg gacttgcaga
    20281 ctgccttgag aggctctctg tgcttcttag gggtgatgat gacgaggcat tgctggaaag
    20341 tgctaagcgg ttgacgtttg aggccgagtc aggatctggg gtggctgccg atgaagatgg
    20401 agttgacctg ggtccgggtt ttgatgaggc tgacttccct gacattgtgg agcttgcagc
    20461 ctacgccggc cgggctaggg agtttgcctc ctcgctccgg tttgcggtta cggctgacgc
    20521 gccgggccat gtcttctggg cggaggcttc cggcgcaggc ccgcgatcga atgtgcgggt
    20581 ggtggctgcg ccgattgaag cggggaaagc cattgccgag cacgtgttgg gtacgcagga
    20641 gtccgtggtc ttcacttctg cgactttggc cgtgggaacc agcttcgact acatcattca
    20701 cgacctaggc ctcgatcttt tggatggtga ccgcgtccga cagatggcga tcgcggcgcc
    20761 gtttgattac gggcggcaag cccttctggc cgtggtggat gatattccta accctgactc
    20821 gcacggatgg gaggcggctg ttgctgaggc cgttgcgtcc atcttggatg cgtctgatgg
    20881 tcgggcattc gtcctgttca cttcgtacaa gctactaagt gaagtggcgc gggcggtgga
    20941 gccacgcctt gcccagcgta acatcgcgct tcttcgtcag ggaaacgcgc cgaggcacgc
    21001 gctcttggcc gagtttaggc gggacgttcg ttctgtgctc tttggcaccg atagtttctg
    21061 ggaaggtgtg gatgtcccgg gcgaagcgct ctcgtgcgtc gtcatgccta ggctgccatt
    21121 cgccgttccc acggcgccag tggtggaggc gaggatagag catattgccg agtcaggcgg
    21181 aaacccattt aatcagtaca cacttcccgc tgcagttctc aagttcaagc agggattcgg
    21241 gaggctagtg cgttcgtcga gcgaccgcgg agctgtggtc gttctcgatt cacgaattgt
    21301 ccgacgccac tacggccgga agttcattga cgcgttgcca ggctgtgcga aatacgttgg
    21361 cggctttgaa gatgttagcg agcggcttta tgaatggctc atcaatccct caccttgagg
    21421 agatggaccg cagtcaggtt agaactcgat tttcgcgacc atggcagtct cttcccccat
    21481 gctctctgag gcctcgccgc gtcatgatct tgcggcactc tacattatct catattctat
    21541 accccgtccc tttgcatatg tattagtggc aacaatcgcg agaagggtga tttcagtggt
    21601 gttcttccgt atattgagca aatggcggac gctccttgac gtggcatgtg tggcttttgt
    21661 tttatctttc gttctaaccc tctttggaaa cgccactgcc gaggtgggta tgggtgtgat
    21721 ctctgcggtg agcacaggcg gcagactcgt tcccatatac tccgttgctc gcgacgacaa
    21781 gaccgtatcg gtaacggtcg atgctacatg gggcgacgac aagacactgg ctctccttga
    21841 cctcttcgat aagcacgaga tcaaggtcac attcttcctg gcaggcaact ggataaagtc
    21901 ttatccggaa aaggtcaagg ctatagccgc gcgcggccac gaggtgggta atcactcctg
    21961 gacccatcca cacatgggcg gactgtcatc tgcacaaatc agggaagaac tggaacgcac
    22021 ccaatctgct ctgtgcgacc tgactggggt gaggcctacc gtgtttcgtc cccccttcgg
    22081 cgaatacacg aactccgtaa taaccatcgc cgaagaatgc ggatgcaaga cgattcaatg
    22141 gagtatcgat tctctagatt ggaaagacct gactgcagac cagatttatg atcgggtaat
    22201 gaaacgggtg gggcctggat cgataatcct ctttcacaat gccgggaaac acacgccgga
    22261 ggcgctcgac aggataatcc gggatctcaa ggcccaaggc tacgcggtgg ttcccgtatc
    22321 ggagctcatc tactcaagcg actactacat agatcgaaac accggggaac aaaggccggt
    22381 caaggtgaga actcgcgcag agccgagttt accccagcaa agcccaccga catctgattc
    22441 gtcgtcgggg ttgcgtcggt agaggaagtg gcgggcaatg aacgacgaaa gcctgtagaa
    22501 atcgatgatg cgagcgggga gcgcccaatc caaatagagg gtgggaggaa cggcgacatg
    22561 atcttcatca ccattaggcg acggacgctt atcctcatag tcctcggggt cattttggct
    22621 atcgtggctg cgtgtatagt ggtaagcatg cctcgaatgc gtcgggctgc ccttggagca
    22681 aagcctgggg tgagagtgcg cgatgtggac gtgggcgggc tgtacaaaga agaagtaagg
    22741 agcatcgtgt tggggttggc gggcaaggtc gaaagaagcc ccaaggaagc gatgtatcat
    22801 gctgagacag gggagatagt tccggaggaa cccggcatta tagtagatat cgacaacacg
    22861 gtgacgtcag tcatggcggc gccgcggggg gccgttttag acctggttac gcggcaggtt
    22921 ccccccgcgg taaccgccgc gatgctcaaa ccgatataca gggttgatac gtcggaaccc
    22981 gcggtggctt tggcattcaa cgtggcatgg ggagaagaat gggttccggc aattcttgag
    23041 attcttgctg aagctggcgc gaaaagtaca ttcttcatta ccggaacatg ggcagcaaag
    23101 tttccccatt tggtccaggc gatggtcgca gcagggaatg agattgccaa tcatggctat
    23161 gatcatccgc accctgaaaa gctctccgag agcgagttag tggcgttgat cgagaagaat
    23221 gagactctct tgagagaact cactggagtg cgcaccaggc tctttgcgcc tccctatgga
    23281 gaggtcaatc agagaattgc ggctgtggcg ggccgattgg gttatgccac gatcatgtgg
    23341 actgttgaca ccatagattg gcaacgcccg gaaccggggg tcatagtgga aagagcggag
    23401 cggcggttag cagggggaaa cattatcttg gtccatccca ccgagcctac tgttgcagcg
    23461 cttcccgtga ttctggagcg atttgcggaa aagggctaca aggtagtgac tgtgtcacgc
    23521 ctcctcgagt tgggcccggc ggtacacacg aggtgaaccg tacgctccgc tagttctgca
    23581 attcccgttc tataaccttc cagtaacccg catcttcctg cccgagtcgc catatggcga
    23641 tcccgccaag gtcgtgcttt ttgacaagct tcagcttggc gctcatgcta tctgcgcttt
    23701 caaaccatac ttcccttgtc acgttgcccg acttgtagcg gaagtatggg ctctgtgaag
    23761 cgttatccca tgaaatcttc gtcccgtgtc gttctgccgt cctaatggcc tggctgtagg
    23821 agatcgcttt cgcacggttt gtactggtgt tccagtcata gccgtaggcg gcgatgccca
    23881 ccaagatctt tttcttcggc atctgtgtca tggcgtactt gatcaccttt tctacccatt
    23941 gcacagacgc gacaggccct ggccccgtgc cggacgtgtg ttgatcgtag gtcatcagca
    24001 ttatctgatc gcatactttg cccagggcgt agtagtcgaa tgcgccaatc catgcagatg
    24061 acggagaatc cttatacttc gctggcaccg acattgtcac cttgtatccc gccggcccca
    24121 gcttcgacct gagctcctgc atgaactggg tcagcactct tctgtctcca ggaggaacgt
    24181 tttcgaagtc gatattgact ccatcatatc catttttctt aacaagatca tagatgtccc
    24241 tgatcgcccg ctctctcgca gccttcgagt tgagcatcga atggatatcg ctctggttaa
    24301 acgatttgcc attaagattg tggacaagag cgaggttgct gagtccacgg gaggaggcta
    24361 tttccatggc gtcatgaaaa tgctcgccag tgacgttccc ggctctgtcc acggcgtatg
    24421 aaaacggcgc tatggccgtc agcgaccttg agtgggagtc gaggacgttg tatgacgact
    24481 tgtcgcctgg atagttcaca gtgtagtatc ccacaacatc gtgcttaagc tgcgcgggcg
    24541 cacctgaggg ctgtccaatt cgcagcaatg atccggcgac ccatccatag cctccgtttg
    24601 ggagcgatac ctgataccac ccgttggcct ggctaaccag gcgcacaggg gtgttcttgg
    24661 cgagcttgcc ggttttgggg taattcgttc ccgggccgga acggacgttg gccgtttccg
    24721 aaatggctgc gctgcggctt gcaagatcca gtactgcgtt tttttctgtt tggggggtgg
    24781 gtgatgtagt ctgtataccc ggagccgttt gaacgcccgg atttgtcttg ttggtgcgca
    24841 tgcctgcaat agagcttgca atgtacatga gaatgaccaa tgctgttgca gtggtgttag
    24901 gttgcaccgg gcgccctcct ctccaggtag acgtaatatt gtatcgtctg ccgcagcgca
    24961 gctgagagag gatttcgtcg ctgcctcctt atctccttca agtaaatagg ggctggaata
    25021 gagatagctg gaaatatcaa tcgaccgtta gtgcacgaaa aaggctgtcg gagcacacat
    25081 ctttttcgat gtgtttatcc aaccttcgca ggacttctgc tcggtagaaa gaattcaaat
    25141 gtgttgggga tgcggtatct cggaggtttt tgcctccctt tgccacaaat cttcgcatat
    25201 tgaacgtgtc aaggaagtga attctaattg tcagaacgcc aagactgtta tggagaaagc
    25261 tctaagcttt gcaacgctca ggactcgcgt cctgttgtaa gggtgatgcc cgttatagat
    25321 gaacggaggc cttgtccgtc acttccagga tacatgagcg ggtcggctgc gggaatcgat
    25381 cttatggcgt gccttgagga taaggtagag cttgcgcccg gagaatacag ggtgattccg
    25441 acaggcctca agatggctat tcccgaaggg tatgagggtc aggtgcggcc tagaagtggg
    25501 ctggctgcta agtttgggat cacggtcctt aattcgcctg gaaccattga tgcagattac
    25561 cgcggcgaaa tcggtgtgat actaataaac catggatttg aaccgtacac tatcgccaat
    25621 ggcgaccgaa tagcgcagct tgtgatcgcc ccagttgtca aagtggagct cctggaaaca
    25681 gctgagctgg atgaaacaga gcggggagaa ggcggattcg gaagcacagg cgtttccgat
    25741 ggtgatggaa agaggtgcac tgatggctga ggccatagca gccagacttg aacagttgaa
    25801 aacggagatc gaggaattga gggccgagct gcgtgagaca gcgacgcgtg agggtgccat
    25861 agccgatctg gacagagcat gttcagtatc agatcggctg gacgcgctta tcctcgactt
    25921 ccttagagtg aaggatcagg ttggtcacac tgttgattga taagcctcac tcgattgcca
    25981 tagcgtcccc gagtccctgc ggcgaaggat tcgaatctaa ggatatcgcc tgcgcgcagc
    26041 ggcagaagcg gttcagaatg aaccatgacc tcctggttaa actaagcgca gtgctcaagc
    26101 ttttgcagga tacaaggagg tcgaatcatg tcgaaggtaa taggcacttt cgcgtcgcgg
    26161 gacgccgcgg aattctgcat tcgggaactg cgaaactcgg gttttgacta caaccgaata
    26221 tcgctggtgg ctagggagtc tggaaaaggc ggagagcagc agggtggcgg gcccaccatg
    26281 gctgggggcg acactgcgct ggagggtacc gctaccggtg gagccattgg aggaatggca
    26341 ggtctcctcg cgggcctggg cgccttggcg attccgggga tcggacctat tgtggccgcc
    26401 gggcctatcg cagcgaccct tactggggct gtaggaggag gcctggttgg aggattagtc
    26461 gatatgggaa tcccctcaga gaggggagag ttttacgaag ggaaggtcaa agcgggccga
    26521 attctagccg tggtcgatgc gggttctgac aaggtgaatg atgctgcgcg catcatgcgc
    26581 gagtacggcg cgtctgacgt agaaacacac taaggaaggc ggctctctgg aagggtggca
    26641 tatctcagca gaactggggg agcgtccagg gcctgcaggg gccgttggca aaactcctcc
    26701 gttgagcggg atgggccggt tgcggcgatg gccgtcaacc ggcccccttg tggaaaaggc
    26761 gcgctcgggg tttcacgcct gcctagtagt gatggggtaa tagatctatg tctgaagaat
    26821 tagacatgtt aacagggcag agcagcagaa gggggctggc ttgggttgaa tcgcgttatc
    26881 aaggtcgtga ttgcgggagc tgcaggaaga atgggccgtg aggttgtgcg ggcgatatgt
    26941 cgtgccgaag acatggagct tgtcggagct gtcgacgtag tcatggcagg acaggacgcc
    27001 ggaacggcgg cggggatagg cgaggtcggg atcgccattt gcactgatct tgccgaagtt
    27061 ctggatagaa ccgctcccga tgtgatggtc gattttactg tggccaaagc cgctgaagcc
    27121 aacataaaga tggccatatc tcggaaggtg cgattcgttg tcggcactac agggatgtcg
    27181 gcgtcgacga tggaatcgat catgaatgaa gcggcccagg caggggtggg gggagccgtt
    27241 gttcccaatt tcgcccttgg cgcagtattg atgatgaggt ttgcgaggga ggcgtcgaga
    27301 tacttcccgg actgtgaaat agtggagctt catcatgacg gcaaagttga ttttccgtca
    27361 ggcaccgcga aagctactgc ccatgaagtc gcatccggga ggggcgacgc tgtctcccgg
    27421 agggggcttg atggaggcat taggggcgta gaggtagagg gcgtcccgat tcattcggtt
    27481 cgtttgcctg gcctagtggc gcaccaggaa gtcatattcg gcggtccggg gcaaaccctt
    27541 actgtgaggc atgattcgct gggccgtgat tccttcatgc ccggagtgct tctagctgtt
    27601 cgcaaggtca gtcaattgga ccatgttgtt ctggggcttg atcccctgct agagtaagct
    27661 ctgtggcgca taggggcctt tttgggcttg ttgcgagccg cgtttgaggc acgcacccat
    27721 gtctatcccg ggcctgcgct gtgcgcggac agacacctcc atatgacatc ggccatatac
    27781 tgcaatcggc gatgttcatg aacgaacgcg cgccgggtgg aaagggacga ttgatggagg
    27841 tgtaagttga tggcactgga agggaagacg attgggattg cccttaccgg ttcacattgc
    27901 actatccccg aggtgctgcc tgagatagag aagctcatca tggggggcgc tgctgtgatc
    27961 ccaatactct cggagtcggt tgcccatact gacaccaggt tcgggcgggc acaggatatc
    28021 cgcgagaaac tggagaagat gacgggaacg cgggttattg atacgatgac caaggccgaa
    28081 ccggtagggc ccaaaaaact cttggactgt ctggtaattg cgccgtgcac tggcaataca
    28141 gtcgcaaagc ttgctctggg cataacggat tctccggtgt taatggcggc caaagcacag
    28201 cttcgcaatg ggagacccgt ggtaatcgga attgcaacaa atgatggcct gtcagggtgc
    28261 gcacgcaata taggcatgct tcattcgcgg aaaaatgtat attttatacc atatgggcag
    28321 gatgatccga ttgggaagcc gacttcgatt gtgtcgcgca tgagcgagat ggagaggaca
    28381 gtggaggaag ctttggctgg tagacaagtc caacctttgc tcgtcgaaaa gtggaaggac
    28441 ttgcgccgtt cgtagcagga attccagggg ctgtcgccaa taagtatgcc taatcggcac
    28501 gcgccaggca gcttcatctg cctgaagtca tttttgatgt gcatgttcat acaagttgtg
    28561 ggggttgatg ccagttgttg agagtagcag ttgtgggcgc aacaggtgcc gtcggcagag
    28621 agatagtctc tgccatattc gagttggagt ttcccgtgtc agatttgatt cttgcatcgt
    28681 ctcccagatc agcggggagc aaggtttcga cgcccatggg cgagatcacg gtgacaaata
    28741 ctgagatctc tgccctggag ggagcagacg ttgcgttcct tgccgcgggt gccagcgtga
    28801 gccggaaatt ggcgccggag ctggcgcgcc ggggtacgct cgtcatcgat aactcgagtg
    28861 ctttcaggat ggatcccgag gttccccttg tggttcctga agtcaacatc gatgctgtcc
    28921 acgagcatcg cggcataatt gcgaacccga actgttcgac cataatagca gtggttgcag
    28981 tggccccaat ataccgccgt tggggattgg agcgtatgat cgtgtcgacc tatcaggcgg
    29041 tgtcgggagc cgggcatgct gcaatagagg cccttgcgga cgagtcagaa gcggtccttg
    29101 caggcttgga tccagtgccc tcggttctgc catacaaatc cgccgaggta catcatcaga
    29161 tagccttcaa tgtcattcct cacgtggact cgttctgtga ggacggatat acccgcgagg
    29221 aatacaagat gaccgatgag atcagaaaga tactgcaaga tccctcgctc aagatatctg
    29281 cgacttgcgt ccgggtgccg gtcttccggt cccattcgga gtcgataaac ctgcagactt
    29341 gccaacgggc tccgatggat gaagtcagag ctgtgttggc ttcctctccg ggcatcaggt
    29401 tgatggatga tcctgtgagt ttcgcatatc caatgcccat acatgtatcc ggcaaagatg
    29461 atgtcgaggt tggcaggttg cgcgaggatc catccgcacc caatgccatg tggctttggg
    29521 tcgtaggtga tcagctcaga aaaggcgccg catccaacgc tgttcagata ggattggcgc
    29581 tctatgatga ccggaggtga cgcgtgtggg cattattgtt cagaaatttg gaggcacgtc
    29641 ggtggcggac gcccaggcgt gtgcgctggt ggtttcgagg atagcggagg cgaggcgcgc
    29701 gggcaatgac gttgttgcag tagtatctgc aatggggcga aaaccggctc cttactccac
    29761 agacgccctt ctggcgctgg tagattgcgc tggagatact ctcccgccta gggagcgtga
    29821 cgccatagca gcgtgtggag agattatatc ctctgtggtg cttgtatcgg ctcttgttgc
    29881 gtcgggctgc cctgccatgt cgtttaccgg ggcgcaggct ggaatcgaga cagacgacaa
    29941 tttcggcgat gctcgcatag tgaggatcga ccccgcgggc ctccacaatg cgattgcaag
    30001 tggtgtcgtt cctgttgtgg caggattcca gggagtcacg gatgggggcg acgtaacaac
    30061 gttgggacgg ggcggaagcg acaccaccgc cgcagcgctg gcagctgcgc ttggtgcgga
    30121 tctggtcgag atatacacag atgtagaggg cgtgatgacg gccgacccac atatcgtgcc
    30181 aaacgccaga ttccaggaga ccatgacgta cagcgagctg atagagatgg cgaacttggg
    30241 ggcgaaggtg gtccacttac gggcggttga gattgcgagt caggcgcgga ttcctctggc
    30301 gatcaagagc gttttttcgg attgcccggg aactttggtg actgacattc cgccacaacg
    30361 aatgcctatt gcggtgccag accggtttgt ttcctctgtg acgtgcatag gcaacatggc
    30421 ccagatagtg cttgaggaat acgatttcaa agctgatgac gctgcactca acgtcttttc
    30481 taccatggcg gataatcata tctcagtgga catgatcaac gttttccccg acatgatcgc
    30541 cttcatcgtc tctgcaaggg attcggcgcg tgctgtgcgt gccctcgaca ggctgggcct
    30601 ttcggcagtg gctaatcctg gttgcgcaaa ggtgtctgta gtaggcgagg gcatgagagg
    30661 agttcccggg gtcatgtcca gggtagtgag ggctcttcac ggcgccggaa tacgaattct
    30721 gcagacctct gactcccatt actccatctc ctgcctggtt ccggaggtcg atatggagcg
    30781 tgcggcgcgc gcactccatt ccgaatttga gctggagaaa taagttcgca gttggcgatc
    30841 tgggagggaa ttacattgaa gaagaacgga agacgcattc gcgtgatcac ggcaatggca
    30901 acgcccttcg cccctgatct gtctgtggat tacagaaaag cgcgagagct cgcaagaagg
    30961 ttaatcgaga acggttcgga cgggctcgtg gtctccggga cgaccggcga atctccaaca
    31021 ttgacgcatg aagagaaact cgagcttttc cgcgtagtct tggaagaggt gggcggtgaa
    31081 gcggcggtaa ttgcagggac cggggggaac gatactcgca cgagtataga gctgacgcag
    31141 gaagcggaga agttgggcgt ggatggcgca atgttggtta cgccctacta taacaagccg
    31201 ccgcaagcag gattgtatcg gcactttgcc agtgtagccc gatcgacttc actgccgctc
    31261 atgatataca acgttccagg caggacctca gtcaacctca gcgccgatac gatggccaag
    31321 ttagccaatg atttttcgaa catagtggcg gtcaaagagg cgtcgggcag cctcgaacag
    31381 gcagctgaga taagatcgaa agcgccaaag ggattcacaa tatacagcgg ggatgacaac
    31441 atgaccatac ccatgatgtc tgtgggcggc gatggggttg tgagcgtggc gtcgcacgtg
    31501 gctggccctg ggattcagca gatggttaca gcgttcatcg acggagatgt ggagcttgcc
    31561 gcagagatgc accgacggtt gttgccatta ttcaaggtga tatttattac cactaacccc
    31621 attccgatta aggcagcctt gaagcttgca ggatttgatg cgggcggact aagaccgccg
    31681 ctggttgaag cgaccgatgc agaacttggg caaatagaag cggtcatgga gtcgcttggg
    31741 ctgatctaat acagtgcata ttggcagggt catacgtggt gatggcctac cagacgtcaa
    31801 caggatccgc gttatcttca ctctgctttc agtcttggaa tcagacaatc gagcgcccta
    31861 tgtccgtgca ctgggcgctc tttgggcttg ctgtttcggc aggcaagtca cctgtgcgag
    31921 gtgttcgttt ctggcctcgg ctcgtatgaa gttgatcatt agatgaggcc gggtactggc
    31981 gcggagcgat ttctacgcct taaagcagaa atcagcgtct tcatgcacgg ccgacaatca
    32041 tacataaagt caacagctac taagtgactc ccaataagtt tttgcgctat cggttagcct
    32101 gtcatctgat gtctgctagg cgtagacacg tctttgcacc tatgtcgcag tgcggtaatt
    32161 g
//
LOCUS       DAOSYL010000022        32030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1140_length_32030_cov_7.588585, whole genome shotgun sequence.
ACCESSION   DAOSYL010000022 DAOSYL010000000
VERSION     DAOSYL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 32030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32030
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_32030_cov_7.588585"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLU15_05220"
     CDS             complement(<1..461)
                     /locus_tag="PLU15_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30355.1"
                     /translation="MEPIPVSLAPGEYEVVKTEYRIHAGMYVSFRRVIGKAVTDSKWQ
                     VTVQSSEYPKWVEIGWVWPRSDGTAPTPTVRKLTGLERGRTFGPLYGGNDPEETDWTA
                     PWLSREVLRELRTGRVAYNFKMGTFSLTGVLADDLRVEEEVLYPIDLNGRRV"
     gene            complement(717..2309)
                     /locus_tag="PLU15_05225"
     CDS             complement(717..2309)
                     /locus_tag="PLU15_05225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO30356.1"
                     /translation="MKRYLFTGGKVYTASARQPFCQALACVGKRIIAVGSESNVLSMT
                     DDTFERIDLRGKVLIPAFIDSHIHFKFYSMCQTSANLEGARDPHDAALRISKAMEGIK
                     PGAWVLGWGWNKNEWTQGRLPDRSYLDSICPRNPVALFSKDLHALWVNTMALRLAGIG
                     LDTPNPEGGEIVRDEDSRQATGVLKESAVELVTRVIPEPTFDAVVLAMQKGQSELHKL
                     GIAGICNMEDALAFEALQAMRSSGRLKLRVTQAIPKQNLDDAIDLGLGTGFGDEWLRV
                     GPVKLFADGALGSQTAHLLRPYEGNRENVGIAVTPKEELAELVGRAVLAGLSCAVHAI
                     GDRANRDVLDIFESVKEESAARHLHHRIEHAQLLHPEDVRRFANLGVVASMQPVHILT
                     DIPVAEKYWGRRSRWAYVFRSLTKAGTPLAFGSDAPVETPDPIRGIYAAVARRGLDGT
                     PDSGWYPEERLTVEEAIRAYSVGSAMASGERMGAGVLVPGSYADIAILSKDIFSIPSS
                     DILDVRVVGTMVGGEFVYRDSI"
     gene            2498..3349
                     /locus_tag="PLU15_05230"
     CDS             2498..3349
                     /locus_tag="PLU15_05230"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOO30357.1"
                     /translation="MSIDRILVVGAGQMGSGIAQVAAEAGLEVSLADLSVDYARKGVD
                     RILKSLNRQVEKGRLTEEDKEDTVSRINCVPDYSAGGDVDLVIEAIVEDPKVKSKLFA
                     ELASICPPEAIFASNTSSVSITSLARDSGRPDRFIGMHFMNPVPVMQLVEVVRGLATS
                     DETCEIIVDLARRMGKEPVVVADSPAFIVNRMAIPMINEAIFALSEGISDAEGIDACA
                     KLGLNHPMGPLALGDLIGLDTVLSILEVMFQETGDPKYRPCPLLRRMVAAGRLGRKSG
                     RGFYEYS"
     gene            3379..4143
                     /locus_tag="PLU15_05235"
     CDS             3379..4143
                     /locus_tag="PLU15_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOO30358.1"
                     /translation="MSKDCVKLQFDDCVATITISRPTALNALNNTVLAELASAIEEVG
                     ENRACRALIVTGEGSKAFVAGADIQEMASLTPDEAVAFSRHGQRVFSAIERLPIPTIA
                     AVNGYALGGGCELALACDIRLASENARFGQPEVGLGIIPGFGGTVRLPLAIGRSRAAY
                     MILTGRHVSAREAYEMGLVSEVMTLESLLPRAEELAREMATKSHVAIGYAKRLLFPDD
                     EALAREAQRFGQCFATDHPKEGMSAFLERRPAKFSD"
     gene            4364..7165
                     /locus_tag="PLU15_05240"
     CDS             4364..7165
                     /locus_tag="PLU15_05240"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOO30359.1"
                     /translation="MIFFRARARVAVPALVLLSLVVFSSSVFGESATGTFDVLFSDSV
                     VVPIGNGVTFERRTSLTSSGWIQMSVIRVNLSSPGIAVGPVLADGSLTSPKAVTSIAD
                     AAGLIAAINGDFFDIGATSAPLSFLAQGGKMIRSPRKDPDFASLVIRDDGTGLLGQWR
                     WEGVLRGPNGLEITLAGLNEISVPADLAVIYDQHWSWARHPGNADVWHLMIRDGIVES
                     CASGWPTPRLSSPGDGNGASDAPRGWLGTPGEAGLTIDGDACPSTPSYDGVLYVVLRG
                     YSAQIAQALSPGDRVSIEGSLTPPTPGLWTAFSGKPVLVQDGAIVRGLWQYTGIQSLL
                     AAPRTAVGLSEDGTTLIMAVVDGRLPGARGVTLDELAKIMISLGAHEALNLDGGGSST
                     AAILDPVTDCVILANRPSGGVQRRVPYAVGVWDTTPQPANDDTDKIDRGKLEQPIPAF
                     ISVSVEAERTGNGMGDSGAPEAIDKPVRTRLGSFPSIEAAIAGTPSFLAGPEVSFDTH
                     SKAIFTVTMYDALGRRMDSADIPDDVEIIWEAIPPKNLRGMPLPEELWSVSEDRLSAV
                     LFGSAPGRFEITVRAERSPAPEEDQLPEIGDGCLPFAQIRLAARAVDPWPMGAASGST
                     PTKEAMNRLALVDDFEGRTSWTGAASSTAVRSVVTTANVEGPTPDFPAPTVDQVDNLA
                     TPSGAVIVLEYDLRSPEQTRAAYIRPSSPVALPSGTSAIRMWVYGDGAGHWLRATVQD
                     LLGQETPIDFPRISWIGWRVVEAPIPQTLVPPLCLSQVYVVEFKPELQAAGRIAIDNI
                     AAVLAPTVPQPAGQAVDTLLCSAPGRQIVRIDGVRLPLPWPDLLAEFEQFRLGNDLEL
                     VVVLRGTNAVDIDTLGVGAALTDPLEAKLLLEMLKGLADGTTSVVLVQDAPTDRKLTS
                     DMEAMIDGVKVIWRKSSPQ"
     gene            complement(7437..7829)
                     /gene="rpsI"
                     /locus_tag="PLU15_05245"
     CDS             complement(7437..7829)
                     /gene="rpsI"
                     /locus_tag="PLU15_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOO30360.1"
                     /translation="MAQLQYAATGRRKCSVARVRLTPGTGKITVNGKPSEEYFGRQTL
                     ELLAREPFEVANRMDRYDAIADVSGGGLSGQAGAIRHGIARALCEGEPELRPVLKKAG
                     LLTRDSRMKERHKYGLKKARKAPQFSKR"
     gene            complement(7855..8292)
                     /gene="rplM"
                     /locus_tag="PLU15_05250"
     CDS             complement(7855..8292)
                     /gene="rplM"
                     /locus_tag="PLU15_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOO30361.1"
                     /translation="MSRTYTPKAEEISRKWYVVDAEGKPLGRLASEVAQILKGKHKPV
                     YSPHMDMGDHVIVINADKVVLTGNKRQTKIHYWHTGYPGGIRQMNYEEFLSTKPEKAV
                     EKAIVGMLPKNRLGRQMAKKLKVYAGPEHPHAAQMPEPLEITV"
     gene            complement(8404..9270)
                     /gene="truA"
                     /locus_tag="PLU15_05255"
     CDS             complement(8404..9270)
                     /gene="truA"
                     /locus_tag="PLU15_05255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOO30362.1"
                     /translation="MRNIGLVMEYVGTGYQGFQVQPAFGRGGESRKGLEGRGGRLIRT
                     VQGEVERAIHRLTGESVRIVGAGRTDAGVHALGQVANFKTESRIPADRFGAALNSVLP
                     EDIRIIRSFEAPEDFNARYDAEWKIYRYLIVPEAARVPAARSGGALLSKRALLTRNPL
                     TDEAVQAMAEAGVALVGRHDFSAYAASGSSARTSVRTIHRLEVNTDRWESLGLNLVVV
                     EIEADGFLYKMARTIVGALLEVGMGRMDSEGPGRLLELRDRKLGPATAPADGLYLMKV
                     AYPEPIGGLASP"
     gene            complement(9272..10066)
                     /locus_tag="PLU15_05260"
     CDS             complement(9272..10066)
                     /locus_tag="PLU15_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO30363.1"
                     /translation="MDFANAAVGQYMPGDSFMHRLDPRTKMLTSLGVVIGLFVVSEPW
                     AYALLALPIMLGIMVSQVPFKLITRSLRPLMFIMVLTFALHLFMDPGEAIFSIGRITA
                     TYEGLRLGLITVVRLALLIMATSIVTLTTSPIALTDGLERLLKPFARLGLPAHELAMM
                     MTIALRFIPTLLEETDKIMKAQMARGADFESGNIAKRARAMVPILVPLFVSAFRRADE
                     LAVAMEARCYRGGEGRTRLRELAFSGADVAVLVVVGAYTVAVAVAL"
     gene            complement(10053..10937)
                     /locus_tag="PLU15_05265"
     CDS             complement(10053..10937)
                     /locus_tag="PLU15_05265"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOO30364.1"
                     /translation="MRGTPLARQALTDVNLSIDEGDLVAIIGKTGSGKSTLVQHFNGL
                     LKPDSGRVLVDGQDIWGKGVALRDIRRKVGLVFQYPEHQLFEETVALDIAFAPKNLGL
                     SDAQVDDAVREAMRIVDLDYEEFADRSPFELSGGQKRRVAIAGVIAMCPKVLILDEPT
                     AGMDPRGREDVLGEIQALHEKHGYTIVLVSHSMEDVARFAKRLVVMEAGKVVMDGDPR
                     EVFRHRQELIDIGLGVPQITDLMAELARRGYGFSTGAITVEEAREILLPPLLAASIES
                     TGSAGPDPCEGRCGHGLR"
     gene            complement(10963..11838)
                     /locus_tag="PLU15_05270"
     CDS             complement(10963..11838)
                     /locus_tag="PLU15_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOO30365.1"
                     /translation="MKPVVRVENVSYAYDGGSQGQVEALLDVSLEIWPGEFVAIIGHN
                     GSGKSTLAKHMNALLLPTRGDVYVLGMNTRDESLLWDIRQTVGMVFQNPDNQIVATIV
                     EEDVAFGPENLGVPPDEMRRRVDEALALVDMTGYEKSAPHMLSGGQKQRVAIAGVIAM
                     RPRCIVLDEPTAMLDPVGRSEVLRTVRHLASNEGIAIVHITHFMSEAVYADRVIVMEE
                     GRVALSGTPREVFSQPATLRKLDLDVPQVTELAAQLRAAGVALPRYPLTVEEMADDIG
                     TLLGRGPCSSELQNH"
     gene            complement(11845..12231)
                     /gene="rplQ"
                     /locus_tag="PLU15_05275"
     CDS             complement(11845..12231)
                     /gene="rplQ"
                     /locus_tag="PLU15_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOO30366.1"
                     /translation="MSRRKLSRTAEERRALFRGSVTSLFLHDKIITTEAKAKEIRPVA
                     ERLITWAKVGDLHHRRLAAKYVLDPVALQRLFDEIGPANIDRPGGYTRIIKLGQRRGD
                     GAPLAIIELVDMPGSSVSAKPAKAKK"
     gene            complement(12251..13234)
                     /locus_tag="PLU15_05280"
     CDS             complement(12251..13234)
                     /locus_tag="PLU15_05280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOO30367.1"
                     /translation="MIESPKPRIDCQEDGDRYGRFVVEPLERGYGITLGNSLRRILLS
                     SLPGAAVTSVKIDGVLHEFSTIPGVYEDVTDIILNLKSLLVRLHTDVPKVIRLMAEGP
                     GDVVAADFEADAEIDILNPELHIATLGTVEAVNQDTGKTEERGAVLSLEAVVEPGRGY
                     VSSEKNKRPEQPIGVIPMDSIFTPVEKVNYTVENARVGGVTDYDRLVLEVWTHGTITP
                     KEAVSMAASMMNEHMKLFISLSEPEEEVEPEAEPEEDCRDRALEMVIEDLDLSVRSYN
                     CLKRAGINTVDELTHKTEEEMLKVRNLGRKSLEEVKQKLESLGLSLRQPDE"
     gene            complement(13272..13895)
                     /gene="rpsD"
                     /locus_tag="PLU15_05285"
     CDS             complement(13272..13895)
                     /gene="rpsD"
                     /locus_tag="PLU15_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO30368.1"
                     /translation="MARYTGSVCRLCRREGAKLFLKGDRCYTEKCAVARRAYPPGEHG
                     QGRKRTSEYGLQLREKQKLRRIYGVQEAQFARYFDMAERRRGITGENLLAILETRLDN
                     VVYRLGLGASRAEARQLVRHGHIYVNGKRVSIPSYELRAGDEISVRPGSKNLVHFKNL
                     AEAGAVTVPAWLSVNEDRLGGRVLSVPTRDQIDLDIQEHLIVELYSK"
     gene            complement(13919..14308)
                     /gene="rpsK"
                     /locus_tag="PLU15_05290"
     CDS             complement(13919..14308)
                     /gene="rpsK"
                     /locus_tag="PLU15_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOO30369.1"
                     /translation="MAKRQARPRRRERKNIETGTAHIKSSFNNTIVTITDPAGAVVSW
                     SSAGAMGFKGSRKGTPFAAQQAADAAARGAIDQGMRSVEVLVKGPGGGREAAVRSLQA
                     AGLEVSMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(14343..14714)
                     /gene="rpsM"
                     /locus_tag="PLU15_05295"
     CDS             complement(14343..14714)
                     /gene="rpsM"
                     /locus_tag="PLU15_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOO30370.1"
                     /translation="MARIAGVDLPRDKRIEVALTYIYGIGLTSSQKILAETGVNPDTR
                     VRDLTETEITQLREAIDRGYKVEGDLRREEAQNVKRLVDIGSYRGLRHRRGLPVRGQR
                     TKTNARTAKGPKKAIGGRKKR"
     gene            complement(14730..14843)
                     /gene="rpmJ"
                     /locus_tag="PLU15_05300"
     CDS             complement(14730..14843)
                     /gene="rpmJ"
                     /locus_tag="PLU15_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOO30371.1"
                     /translation="MKVRPSVKKMCEKCKIVKRKGRVRVICENPTHKQRQG"
     gene            complement(14855..15073)
                     /gene="infA"
                     /locus_tag="PLU15_05305"
     CDS             complement(14855..15073)
                     /gene="infA"
                     /locus_tag="PLU15_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOO30372.1"
                     /translation="MAKEDAIEVEGTVVEPLPNAMFKVELENGHTVLAHVSGKMRMKF
                     IRILPGDRVTIQLSPYDLTRGRIVWRYK"
     gene            complement(15076..15372)
                     /locus_tag="PLU15_05310"
     CDS             complement(15076..15372)
                     /locus_tag="PLU15_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOO30373.1"
                     /translation="MSSGGPLLGQFVVSKMGRDRGRLFVVVGREGDGYVYIADGATHK
                     LGKAKRKNVKHLIVTEQVDEDIRAALEAGNSVTDEQLVTALRVYRPQERKEGDA"
     gene            complement(15396..16142)
                     /gene="map"
                     /locus_tag="PLU15_05315"
     CDS             complement(15396..16142)
                     /gene="map"
                     /locus_tag="PLU15_05315"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOO30374.1"
                     /translation="MIILKTAAEIEQMRKAGALVGQVLERLGRYVAPGVRTAELDAIA
                     EEMILAAGGTPSFKGYCGYPASICVSVNDEIIHGIPGKRVLVEGDIVSIDCGAILDGY
                     QGDAAMTFPVGDVSVEAEKLMRVTREALEAGIEMARPTMRLGDIGNAIQVHAESNGFS
                     VVRDFVGHGIGRRMHEDPQIPNFGSPGTGLRLRPGMVLAIEPMVNAGGHEVMIMDDGW
                     TTCTRDGSLSAHFEHTVAITDAGPEVLTLP"
     gene            complement(16139..16786)
                     /locus_tag="PLU15_05320"
     CDS             complement(16139..16786)
                     /locus_tag="PLU15_05320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOO30375.1"
                     /translation="MRAVLMGPPSAGKGTQAVRLAAEFGVPHISTGDMFRAAIAAGAE
                     LGRRAQGFIEAGKLVPDDVTLGIVCERLAEADAMSGFILDGFPRTVPQAEGLEDILRA
                     QGVRLDAVVLIEVPEDVLVRRVTGRRVCEKCGRVYHMEWNPPPGSACECGGLVTQRKD
                     DLPETVKERLATYRTQTEPLRDYYASEGSLVVVDGTGDPDEVFRRITRALGRCGS"
     gene            complement(16832..18094)
                     /gene="secY"
                     /locus_tag="PLU15_05325"
     CDS             complement(16832..18094)
                     /gene="secY"
                     /locus_tag="PLU15_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOO30376.1"
                     /translation="MLAALVNAWRVPDLRKKILYSAFLLFVYRLGAFIPAPGVDMAKV
                     AEVFQAVMGETGGGLLGLVDMFSGGSIQRLSVFAVNVGSYITASIVIELLTVVIPSLE
                     ELSKEGPEGRKKISQYTRYLAVALAVLQSYGYSAMFREAIRPGAVWTLLVIVSMTAGA
                     SFVIWLGEKISEKGIGNGISLIIFAGIVTQLPGNVKNVISLVKSGGTGWVFAIVYVLM
                     SLALVIGVVAISEGQRRVPVQYPKRVVGRRVYGGQSTHIPIKVNQAGVIPVIFASSVL
                     AFPGTLAQFIPGMAPGVRAFMNNVWAYNIVYVALIILFSYFYTGITFNPVEVADNMRK
                     YGGFIPGFRPGRPTAEYLDRVLTRLTFVGAIFLAIIALLPNITGRLTSNTVTFGGTGL
                     LIVVGVALDTMKQIEAQLIMRQYEGFLK"
     gene            complement(18095..18538)
                     /gene="rplO"
                     /locus_tag="PLU15_05330"
     CDS             complement(18095..18538)
                     /gene="rplO"
                     /locus_tag="PLU15_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOO30377.1"
                     /translation="MKLHELQPAAGAKRAPKRVGRGTGSGHGKTAGRGSKGQLSRSGG
                     GKGPGFEGGQTPLYRQLPKRGFTNKYKVEYAVVNVSDLERVFEAGDEVDRDALREKGL
                     VKGHDALVKVLGDGELTKSLTVRADKFSESAMSKIKAAGGSAEVV"
     gene            complement(18552..18737)
                     /gene="rpmD"
                     /locus_tag="PLU15_05335"
     CDS             complement(18552..18737)
                     /gene="rpmD"
                     /locus_tag="PLU15_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOO30378.1"
                     /translation="MAKKLVVTLKRSTISRPKDQRATVRSLGLRKIGQTVEHDDTPVI
                     RGMINKVSHLVEVTEID"
     gene            complement(18751..19251)
                     /gene="rpsE"
                     /locus_tag="PLU15_05340"
     CDS             complement(18751..19251)
                     /gene="rpsE"
                     /locus_tag="PLU15_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOO30379.1"
                     /translation="MAKINPDSLDLKERVVSINPVAKTTKGGRTRSFRALVVVGDENG
                     HVGAGLGKAYEVSEAIKKGVDDAKKNLVSVPMVGTTVPHEVTAKSGAGCVMLKPAGPG
                     TGVIAGGAVRAVLELAGYRDILTKSLGSNNPMSMVNATLVGLQSMRTAEQVARTRGKS
                     VEELAR"
     gene            complement(19270..19638)
                     /gene="rplR"
                     /locus_tag="PLU15_05345"
     CDS             complement(19270..19638)
                     /gene="rplR"
                     /locus_tag="PLU15_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOO30380.1"
                     /translation="MYNRVDRKEALARRHQRVRKDVSGTPERPRLSVFRSHAHIYVQI
                     IDDTTGHTLVAASSVEPDIRAKVGYGGNIAAAEEVGRVAAERAIEKGIRKVVFDRGGN
                     RYHGRVAALAEAARKGGLEF"
     gene            complement(19677..20219)
                     /gene="rplF"
                     /locus_tag="PLU15_05350"
     CDS             complement(19677..20219)
                     /gene="rplF"
                     /locus_tag="PLU15_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOO30381.1"
                     /translation="MSRIGKKPVPVPKGVEVELDGSIITVKGPKGALSQEIPARISVE
                     VADGEIRVTRPSDNRLDRSMHGLTRALIANMVHGVTEGFEKSLEIIGTGYRASKQGET
                     LVLQVGYSHPVEIVPENGIQIDVPSPTKITVRGTDKQLVGQTAANIRAVREPEVYHGK
                     GIRYAGERVALKAGKAGKIG"
     gene            complement(20231..20629)
                     /gene="rpsH"
                     /locus_tag="PLU15_05355"
     CDS             complement(20231..20629)
                     /gene="rpsH"
                     /locus_tag="PLU15_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOO30382.1"
                     /translation="MVLTDPIADMLTRIRNANTVNHEVVEIPASRMKAELAQIMKDEG
                     YIRGFEVTRGDKYDVIKVHMKYGPNKQKVIHGLKRISKPGLRVYVKKDEVPKVLNGLG
                     TAVISTSRGLMTDRQAREAGLGGEVICYMW"
     gene            complement(20665..20850)
                     /locus_tag="PLU15_05360"
     CDS             complement(20665..20850)
                     /locus_tag="PLU15_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOO30383.1"
                     /translation="MARKALVEKWQKEPKYKVRQYNRCSICGRPRGYMRKFKMCRICF
                     RTLASKGEIPGVTKASW"
     gene            complement(20863..21417)
                     /gene="rplE"
                     /locus_tag="PLU15_05365"
     CDS             complement(20863..21417)
                     /gene="rplE"
                     /locus_tag="PLU15_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOO30384.1"
                     /translation="MEAPTPRLKEKYQNEVVPALMQKFEYRNVMEVPKVEKVIVNRGV
                     SDAIVDPKALDAAADELANITGQRPIITRAKKSIAAFKLRAGVPIGCKVTLRGARMYY
                     FLDKLFNIALPRIRDFRGVSPDAFDGRGNYTLGLREQLVFPEIRYDDVDKIRGMDITI
                     VTTAKTDEEAFELLKLMGLPVRLQ"
     gene            complement(21451..21783)
                     /gene="rplX"
                     /locus_tag="PLU15_05370"
     CDS             complement(21451..21783)
                     /gene="rplX"
                     /locus_tag="PLU15_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOO30385.1"
                     /translation="MTISVKKVKKGDTVMVLQGKDAGRRGKVLRVLPREQKVIVENLN
                     IVKRHTRPSTANPQGGIIDKPAPMALGRVMVVCGKCNKPTRVGMELGESGKPMRVCKK
                     CGAKLDKK"
     gene            complement(21824..22192)
                     /gene="rplN"
                     /locus_tag="PLU15_05375"
     CDS             complement(21824..22192)
                     /gene="rplN"
                     /locus_tag="PLU15_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOO30386.1"
                     /translation="MIQAQTVLNIADNTGAKKVMCFRVLGGGDRRYAGIGDIIIGTVK
                     EATPGGVVKKGDIVKAVVVRTKKELRRADGTYIRFDENAAVVIDANNNPRGTRIFGPV
                     ARELRDRNFMKIISLAPEVL"
     gene            complement(22223..22483)
                     /gene="rpsQ"
                     /locus_tag="PLU15_05380"
     CDS             complement(22223..22483)
                     /gene="rpsQ"
                     /locus_tag="PLU15_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOO30387.1"
                     /translation="METARNERKERVGFVVSDAMDKTRVVELQRTYKDPMYGKVVRRS
                     SKVKVHDEGNESRVGDKVLIMETRPISRDKRWRLVKIVEKAK"
     gene            complement(22499..22702)
                     /gene="rpmC"
                     /locus_tag="PLU15_05385"
     CDS             complement(22499..22702)
                     /gene="rpmC"
                     /locus_tag="PLU15_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOO30388.1"
                     /translation="MKARELREMTPEQLDTRLEGLKEELFNLRFQLATNQLDNAHRIK
                     DVRRDIARVKTEMRERELKIARG"
     gene            complement(22692..23126)
                     /gene="rplP"
                     /locus_tag="PLU15_05390"
     CDS             complement(22692..23126)
                     /gene="rplP"
                     /locus_tag="PLU15_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOO30389.1"
                     /translation="MLMPKKVKYRKQQRGRMKGVAEKGTTVTYGEYGLQALEPGWITS
                     RQIEAARIAMTRYIRRGGKVWIKIFPDKPVSKKPAETRMGSGKGAPEYWVAVVKPGRV
                     MFEMGGVSEEIAREAMRLAGHKLPIKTQFVKREEAGGEADEG"
     gene            complement(23127..23792)
                     /gene="rpsC"
                     /locus_tag="PLU15_05395"
     CDS             complement(23127..23792)
                     /gene="rpsC"
                     /locus_tag="PLU15_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOO30390.1"
                     /translation="MGQKVNPIGLRLGINRTWESTWYSDKNYAELLHEDLKLRKYIKN
                     RLYAASVSRIQIERAANRIRVTIHTARPGMVIGRGGSEADALRRDLEAMTGKQVSVNI
                     MEIKHPELDAQLVAENIAAQLERRISFRRAMKQAMGRAERLGVQGMRVKSSGRLGGAE
                     IARTEGYSYGKLPLHTLRADIDYGFTEANTTYGKIGVKVWIYKGDVLPDKSGKARQGG
                     GAR"
     gene            complement(23796..24134)
                     /gene="rplV"
                     /locus_tag="PLU15_05400"
     CDS             complement(23796..24134)
                     /gene="rplV"
                     /locus_tag="PLU15_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOO30391.1"
                     /translation="MEAKAVARYVRMSPQKVRRVVDLVRGKSVDEALTILKYTPHIAA
                     EPVSKVVASAAANAENNLDLSRDDLYIAEAYVDEGPTLKRIRPRARGMANRILKRTSH
                     ITVVVREREG"
     gene            complement(24149..24433)
                     /gene="rpsS"
                     /locus_tag="PLU15_05405"
     CDS             complement(24149..24433)
                     /gene="rpsS"
                     /locus_tag="PLU15_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOO30392.1"
                     /translation="MGRSLKKGPYVHEALAEKVERLNSAGKKEIIKTWSRASMITPDM
                     LGHTIAVHDGRRHVPIYIVEDMVGHRLGEFAPTRTFRGHRQSDKTTQIKR"
     gene            complement(24466..25296)
                     /gene="rplB"
                     /locus_tag="PLU15_05410"
     CDS             complement(24466..25296)
                     /gene="rplB"
                     /locus_tag="PLU15_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOO30393.1"
                     /translation="MGVKSFKPTTPARRQMTVATFEEITSSKPEKSLVVPLKKSGGRN
                     AQGRTTVRFRGGGHKRAYRVVDFKRDKDGVPGRIASIEYDPNRSARIALVNYADGEKR
                     YILAPLGLKVGDVIESGPDADIKPGNALPLSNIPVGTVVHAVELTAGRGAQLARSAGA
                     SAQIMAREGEYVTLRLPSTEMRMVRQECRATVGQVGNVEHENISMGKAGRSRWMGRRP
                     SVRGVAMNPVDHPHGGGEGRSPIGLKSPVTPWGVPTLGYRTRKAKASDRLIVRRRGKK
                     "
     gene            complement(25321..25608)
                     /gene="rplW"
                     /locus_tag="PLU15_05415"
     CDS             complement(25321..25608)
                     /gene="rplW"
                     /locus_tag="PLU15_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOO30394.1"
                     /translation="MAHPRDIIIGPIITEKSNALSAEGKYVFKVKRDANKIEIARAVE
                     KIFDVRVVAVNTINVPGKLKRQGRSEGMTPAWKKAIVTLQDGETIPLFEGV"
     gene            complement(25608..26228)
                     /gene="rplD"
                     /locus_tag="PLU15_05420"
     CDS             complement(25608..26228)
                     /gene="rplD"
                     /locus_tag="PLU15_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOO30395.1"
                     /translation="MPKAPLFDARGNRLGEVELPEAVFGIEPNEHAVHEAVTLQLASR
                     RSGTHSTKTISEVRGGGRKPWRQKGTGRARHGSRRSPIWVGGGVVFGPKPRKYGYTIP
                     KKVRRLALLSALSSMAQENAVYVVDAIDLSEPSTKSMAALFEKIGAEKPLVVTDERSE
                     VVEKSTRNIPGAMVLHVEGLNVYDIVNHTALVLTRDAVSRAEEAFA"
     gene            complement(26242..26877)
                     /gene="rplC"
                     /locus_tag="PLU15_05425"
     CDS             complement(26242..26877)
                     /gene="rplC"
                     /locus_tag="PLU15_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOO30396.1"
                     /translation="MGRAIIGRKIGMTQIFEEDGRVHPVTVIEAGPCCVVQKKHTATD
                     GYEAVQLGFGEIDKRRVSKPMAGHYAKANVKPSRHLKEFRIESADAYELGQEIKVDQW
                     SVGDLVDVTGISRGKGFAGPMKRWNFSRGPMAHGSHYHRGVGSLGATAPARVFRGRKM
                     AGRLGGTKATSLGLEVVKVDPERNLLMVRGSIPGATNGLVTIRDSVKAKAK"
     gene            complement(26898..27206)
                     /gene="rpsJ"
                     /locus_tag="PLU15_05430"
     CDS             complement(26898..27206)
                     /gene="rpsJ"
                     /locus_tag="PLU15_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOO30397.1"
                     /translation="MATQKIRIKLKAFDHKILDQSAERIVETAKRMGSEVSGPIPLPT
                     ERNVYTVLRSVHVNKDSREQFEMRTHKRLIDILDPTPKTIDALMRLDLPAGVDIEIKL
                     "
     gene            complement(27371..28573)
                     /gene="tuf"
                     /locus_tag="PLU15_05435"
     CDS             complement(27371..28573)
                     /gene="tuf"
                     /locus_tag="PLU15_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOO30398.1"
                     /translation="MAKQKFERTKPHLNIGTIGHVDHGKTTLTSALTNVLAKNGLAQF
                     MDFDKIDKAPEEKARGITISTSHVEYETPNRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPSIVVALNKVDMVDDPELLELVELEVRDLLSE
                     YEFPGDEIPVIPLSAYKAGECGCGQKDCQWCGPIWKLVEAIDAYFPEPQREIDKPFLM
                     PIEDVFTITGRGTVVTGRVERGVVKVGDEVQIVGLMPQPRKTVVTGVEMFRKLLDQGV
                     AGDNVGCLLRGIDRDEVERGQVLAKPGSITPHTKFTAQVYVLTKEEGGRHTPFFNGYR
                     PQFYFRTTDVTGNVQLPEGTEMVMPGDNTVMTISLITPIAMEPGLRFAIREGGRTVGA
                     GAVASIIE"
     gene            complement(28598..30685)
                     /gene="fusA"
                     /locus_tag="PLU15_05440"
     CDS             complement(28598..30685)
                     /gene="fusA"
                     /locus_tag="PLU15_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO30399.1"
                     /translation="MLGVTPPERIRNIGIMAHIDAGKTTTTERILFYTGRVHRLGEVD
                     SGTATMDWMVQEQERGITITSAATTCDWRDYQINIIDTPGHVDFTVEVERSLRVLDGA
                     VALFDAAEGVQPQSETVWRQADRYRVPRIAFVNKMDRVGADFDMAVRAIRERLGATPA
                     PVQIPIGAEDRFAGMIDLISQKAIVYMDDLGLNRQVVEIPEELRQPAAEARERLVELV
                     AENDDHVMMLYVDGEEITSQDIIDGLRRATLSAKLTPVLCGSAYRNKGVQSLLDAIVD
                     YLPSPMDVPPIVGVDPTNDEEQIERKPSDDEPFCGLAFKIATDPYVGKLAYFRVYSGV
                     AKVGDAIYNASKGKRERFGRILRMHANHREELDLVSTGDIVAAVGLRNTTTGDTLCDE
                     RAPVVLERMEFPEPVIAVAVEPKSKNDEDRLTVSLAKLAEEDPTFRVRTDPETGQTLI
                     SGMGELHLEVIVDRLLREFNVQANVGKPQVAYRETITCPVRHEGRFVRQTGGRGQYGH
                     VVLELEPGAQGSGIVFENKVVGGAIPKDFIPAVEAGIMEAAGNGVLAGYPVVDVAVAL
                     VDGSYHEVDSSELSFKIAGSLAFKEAMERAKPVLLEPIMKVEVETPELYVGDIMADLG
                     ARRGKVTALENVGQNQVIRAQVPLAEMFGYATTMRSLSQGRANHTMHFLRYEQVPDNL
                     AAEIMRRYRGVLS"
     gene            complement(30720..31190)
                     /gene="rpsG"
                     /locus_tag="PLU15_05445"
     CDS             complement(30720..31190)
                     /gene="rpsG"
                     /locus_tag="PLU15_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOO30400.1"
                     /translation="MPRRGTIVKHETVPDPVYNSELMAEFISKLMLDGKKSVAESILY
                     EAADIVQAKTGKEPLDTFERAVKNVMPVLEVRARRVGGATYQVPTEVRPERRKALAFR
                     WIVGNARKRPERTMAERLAAEILDASENTGASVKRKEEMHRMAEANKAFAHYRW"
     gene            complement(31220..31633)
                     /gene="rpsL"
                     /locus_tag="PLU15_05450"
     CDS             complement(31220..31633)
                     /gene="rpsL"
                     /locus_tag="PLU15_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOO30401.1"
                     /translation="MPTINQLVRKGRKQSKAKSASPALRYQYNSLNRRSTYGKGAPQR
                     RGVCTVVRTTTPKKPNSALRKIARVRLTNGLEVTAYIPGEGHNLQEHSVVLIRGGRIK
                     DVPGVRYHIVRGTLDAAGVADRRQGRSKYGAKRPK"
     gene            complement(31718..31966)
                     /locus_tag="PLU15_05455"
     CDS             complement(31718..31966)
                     /locus_tag="PLU15_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOO30402.1"
                     /translation="MNQGLADADSRVIGTKQTVRALERGCVSAVYIASDAEEHVIRDV
                     KALCVRNGVPIIMVDSMEELGKACGIDVGAASAAILHE"
BASE COUNT         6708 a        10151 c         7568 g         7603 t
ORIGIN      
        1 acccgtctgc cgttgagatc gatgggatac aacacctctt cctcaacacg gagatcatcc
       61 gccaaaactc cggtcaagct aaaagtcccc attttgaagt tgtaggcaac tcttcccgtg
      121 cgaagttcac gcaagacttc ccgtgatagc cacggcgcag tccaatccgt ttcctcggga
      181 tcattgcctc cgtacaatgg tccgaatgtg cggccacgtt caagccctgt caacttcctc
      241 acagttgggg taggcgcggt gccgtccgat ctaggccata cccatcctat ctccacccac
      301 ttggggtatt cggacgattg gacagtcacc tgccacttcg aatcagtaac agcctttccg
      361 atcacccgtc ggaacgacac gtacataccc gcatggatcc tgtattctgt cttgactact
      421 tcatattctc caggagccaa actgactgga attggttcca tcggccgtac tgtttgagct
      481 tccgatggcc caacgcatag cacgcacact gaagtcaagc atatcaaagc cagcaaaacc
      541 cttttcttca aaccaatctc cccttgaacg gtcatagttg cggcctggcc caaacaacgt
      601 acgtccagac cggtcccaat attgacacac tctccacttc gcctccgggc catgcccatt
      661 cctcgctatc cgctcagccg cgccaatgcc tcgtatggca gcgcccagat gcttgcctag
      721 atggagtctc tgtaaacaaa ttcccctccc accatggtgc cgaccacgcg cacatcaagg
      781 atatcggatg aaggaattga aaatatgtct tttgacaata tagcaatgtc ggcatatgat
      841 cccggaacca gaaccccagc tcccattcgc tcgcccgaag ccatcgccga gccaacagag
      901 taggccctaa tggcttcttc cactgttagc ctctcctcag gataccatcc tgaatctggc
      961 gtcccgtcca gccctcttct ggcgacggcg gcgtatatgc cgcggatggg gtcaggagtc
     1021 tcaaccgggg cgtcggagcc gaaggcaagt ggcgttccgg cctttgtcag gctgcggaag
     1081 acataagccc acctactcct cctgccccaa tacttctcgg ctacgggaat gtcagtgagt
     1141 atatgaaccg gctgcatcga ggctactacc ccgagattgg caaacctgcg aacgtcttcg
     1201 ggatgcagaa gctgtgcatg ctctattcta tggtgcagat ggcgcgcagc gctctcctct
     1261 ttcacgctct cgaaaatatc gagcacatcc ctgttagcgc ggtcgccgat ggcatggact
     1321 gcacaggaca agcctgcaag aacagcacga ccgaccagct cagccagttc ttcctttgga
     1381 gtcacagcaa tgcctacatt ctccctgttg ccttcgtaag gcctgagcag gtgtgcagtt
     1441 tgagaaccca atgctccgtc agcaaagagt ttcaccggcc ccaccctcag ccactcatcc
     1501 ccaaaaccag ttccaagccc caagtcgatc gcatcgtcca agttttgctt ggggatagcc
     1561 tgcgtaacgc ggagcttcaa ccgccccgaa ctcctcatcg cctgcaaagc ctcgaatgcc
     1621 agagcatcct ccatgttgca gatccctgca attcccagct tatgaagttc cgactgtcct
     1681 ttttgcatag ccaagacaac tgcatcgaat gttggctctg gaattactct agtcacgagc
     1741 tccacagctg attccttgag aacccccgta gcttgcctcg aatcttcatc cctgactatc
     1801 tcgccgccct caggattagg agtgtcaaga cctatgcctg caagacgcaa tgccatggta
     1861 ttcacccaga gagcatgtag atccttactg aaaagggcca ctgggtttct agggcaaatg
     1921 gagtcaagat atgagcggtc cggcagtctc ccttgcgtcc actcattctt gttccatccc
     1981 catcctagca cccacgcacc gggcttaatg ccttccatgg ccttgctgat gcgaagagcg
     2041 gcatcatggg gatcgcgtgc cccctcaaga tttgctgagg tctggcacat cgagtagaac
     2101 ttgaaatgta tatgagaatc aatgaaagca ggaatcagga ccttgcctcg aaggtcaatc
     2161 ctctcgaaag tgtcatctgt catcgacagc acattcgatt cagaaccaac agcgattatc
     2221 cgctttccta cacaagccag agcctgacaa aatggttgac gtgcagatgc agtgtatact
     2281 ttgcctcccg tgaacaagta cctcttcaag aagctccctc ccagcattcc tcgggtgatt
     2341 gactatttca tcaacctttc ctcttctcct gccagaggag cagagaagga gtcaaaagag
     2401 gggctataga agtaataatc gttagtgtgg atcaactatc tccgacctct gcaattggct
     2461 tgcaacagag tgtcgcggat gaaagggtga ttgtttcttg tccatcgaca gaattctcgt
     2521 ggtgggcgca ggtcaaatgg gctccgggat cgcgcaggtc gcagccgagg ccggcctcga
     2581 ggtgtcccta gccgatctat cagtagacta tgctcgcaaa ggcgtagacc gcatactcaa
     2641 aagcctcaac cgacaggtcg aaaaaggacg cctgactgag gaagacaaag aggacacagt
     2701 atcgaggatc aattgcgtac cggactactc agcaggcggc gatgtagacc tggtgatcga
     2761 agcaatcgtt gaagatccaa aggtcaaatc caagctcttc gcggaactgg cgtcgatatg
     2821 ccctccagaa gcaatattcg cctccaacac gtcctcagta tccataacgt ccctggcccg
     2881 tgattcgggg cgccccgatc gtttcatcgg aatgcacttc atgaatcccg tccctgtgat
     2941 gcagctcgtg gaggttgtac gcggcctcgc gacctcagat gaaacatgtg aaatcatcgt
     3001 cgacctcgcc aggagaatgg gcaaagaacc tgttgttgtc gcggattcgc ctgccttcat
     3061 agtcaacagg atggccattc cgatgataaa tgaagccata ttcgcacttt cggagggcat
     3121 atcagatgcc gagggcatag acgcctgcgc aaagctgggg ctcaatcatc ccatgggccc
     3181 gctggccctc ggggatctga tcggtctcga tacggttctg tccatactcg aagtcatgtt
     3241 tcaagagacc ggggatccca agtacaggcc ctgcccgctc ctgcggagga tggtggccgc
     3301 tggcagactt ggacgcaaat caggccgagg attctatgaa tactcctgat tcaaatcttc
     3361 gctaggaggt ggcaaacctt gagcaaagac tgtgtcaaac ttcagttcga cgattgtgtg
     3421 gcaacgatca ccatatcccg cccaacggcg ctcaacgccc taaacaatac agttctggct
     3481 gaacttgctt cggcaatcga agaggttgga gagaatcgtg cgtgccgtgc cctgatcgtc
     3541 accggagaag gcagcaaagc gtttgtagct ggtgcagata tccaagagat ggcaagcctt
     3601 actcctgacg aggccgtcgc cttctcccgg catggccaga gggtattcag cgctatcgaa
     3661 cggttgccga ttccaaccat agccgcagtg aacgggtatg ccctcggagg aggctgcgag
     3721 ctcgcgctcg cgtgtgatat ccggttggcc tcggaaaatg cccgcttcgg ccagcccgag
     3781 gtgggcctcg ggatcatacc tggatttggc ggcactgtac gccttcccct ggccatcgga
     3841 cgttcccgcg ccgcctacat gatactcaca ggtagacacg tttccgcccg ggaggcgtac
     3901 gaaatgggcc tcgtgtctga agtgatgact ctggaatccc ttcttccaag ggcggaagag
     3961 ttagcccggg aaatggccac caaaagccat gtggccatag gatatgcgaa acgcctgttg
     4021 ttccctgatg atgaggcact cgcccgggaa gcccagcggt tcggacaatg tttcgcaact
     4081 gaccatccga aggagggaat gtctgcattc ctagaacgca gacccgcgaa gttctctgac
     4141 tgaaatacag cccgtccagc gcggttggct tatctcgcaa ctctcgctcg atctctgcgt
     4201 tgtctgaggt ccatgactca gctctgcagc gttgaaacac tgttcttgag ttgctcagct
     4261 gccggtgcaa ctgccgggca agggaatgga caactttatg ggaaataaga aaaactgtag
     4321 atgttttcga tccacaaaac atgcaaggat tccaacgcca actgtgattt tctttcgggc
     4381 cagggctcga gttgcggttc ctgcactcgt gctgctcagc ctcgttgttt tctcgtcgtc
     4441 agttttcgga gagagcgcga caggcacatt cgatgtcctg ttctccgatt ctgtcgtagt
     4501 gcccattggc aacggcgtga ccttcgaacg aaggacgtca ttgacctcgt ccggatggat
     4561 tcagatgtca gtaatacgcg tcaacctgtc ctcacccggt atcgctgtgg ggcccgtctt
     4621 ggcagatggc tcactcacct cgccgaaggc tgtgacatcc atagccgatg cggctggatt
     4681 gattgctgcg ataaatggtg actttttcga catcggcgcc acttccgctc cgctttcctt
     4741 cctggctcag gggggaaaga tgatacgctc tccgcggaaa gatcctgact tcgcctcact
     4801 tgtgatccgc gacgacggca cagggcttct cgggcaatgg agatgggaag gtgtcctaag
     4861 aggtccgaac gggctcgaga tcaccctcgc cggcctgaat gagatatcgg tcccggccga
     4921 cctggctgta atttacgatc aacactggtc gtgggcgaga cacccaggca acgccgatgt
     4981 ctggcatctg atgatccgcg acgggatcgt cgaatcgtgc gcgagcgggt ggccgacacc
     5041 tcggctttcc tcgccagggg acggcaatgg agctagcgat gctccccgag gctggctcgg
     5101 cactccgggc gaagctggat tgaccatcga cggagacgct tgtccttcga cgccatcgta
     5161 cgatggcgtc ctctatgtcg ttttgagggg atattcagcg cagattgccc aggcgctttc
     5221 gccaggagac cgcgtttcca ttgaagggtc gcttacgcct ccaacacccg gcttatggac
     5281 tgccttttct ggcaaaccgg tgctcgtcca ggacggcgcc attgttcgcg gtctgtggca
     5341 atacacaggg attcagtctt tacttgcagc cccgcgcacc gccgttggcc tctcagagga
     5401 cggcactacc ctcataatgg cagtagtcga cggccgcctg cccggcgcga ggggtgtcac
     5461 acttgacgaa ttggcaaaga taatgatctc gctcggcgcc catgaggccc tgaacctcga
     5521 tggcggcggt tcttccaccg cagctatcct agacccggtc accgactgcg taattctggc
     5581 caaccgcccc tcaggaggcg tccaacggcg cgtaccatat gcggttggtg tgtgggacac
     5641 aacaccacaa cctgcaaatg acgatacaga caagatcgat cgggggaaac tcgaacaacc
     5701 gatccctgcc ttcatctcgg tttccgtgga agccgaacgc acaggcaatg gcatgggaga
     5761 ctccggagca cctgaagcca ttgataaacc tgtccgcaca cgcctcgggt cgtttccctc
     5821 tattgaagcg gcgattgcag ggacgccctc atttctcgca gggcctgaag tctcttttga
     5881 tacacattcc aaagcgattt tcacagtaac catgtatgac gcgttaggac gacgcatgga
     5941 ttctgccgat atcccagatg acgtcgagat catctgggag gcaattcctc caaagaatct
     6001 acgcggcatg ccactcccag aggagctgtg gtccgtgtcg gaggatcggc tctccgccgt
     6061 cctattcggc agcgcgccag gccgattcga gatcacagtc agggctgaac gatcgccagc
     6121 tcccgaagag gatcagcttc cagaaatcgg cgacggatgt ttaccctttg cccaaatacg
     6181 tctggctgcc cgcgccgtcg acccctggcc catgggcgcg gcaagtggct ctacgccgac
     6241 caaagaggca atgaaccgcc ttgcgctagt tgacgatttc gagggccgca cctcatggac
     6301 tggcgcagca tcttccacag cggttaggtc agtcgtgact actgcaaacg tggaaggacc
     6361 tacacccgac tttcccgccc caaccgtgga ccaagttgac aatcttgcaa ctccctcggg
     6421 cgctgtaatc gtccttgagt atgatctgag gagcccggaa cagacccgcg cagcgtacat
     6481 caggccttct agtcccgtag cgctgccttc gggaacatca gccatacgga tgtgggtata
     6541 tggggatggt gcaggccact ggcttcgcgc cacagtccaa gatttactgg gacaggaaac
     6601 ccccatcgat tttccgcgga ttagctggat aggatggagg gtcgtagaag cgcccattcc
     6661 tcaaacgcta gttccgccac tctgcctttc ccaagtgtac gttgtcgagt tcaagcccga
     6721 gcttcaggca gcaggaagaa tagccattga caacatagct gccgtcttgg ccccaaccgt
     6781 ccctcaaccg gccggtcagg ctgttgacac acttctctgt tcggcccctg gtcgacagat
     6841 cgtgagaatt gacggggtgc gattgccgct gccatggccc gatcttctgg ccgaatttga
     6901 acaattccgg ttaggtaatg acctggaact ggtggtagtt ttgagaggga caaatgccgt
     6961 cgacatagat actctcggcg tcggtgcggc actcacagac cccctcgagg caaaactcct
     7021 gctggaaatg ttgaagggct tagctgatgg cacgacctcg gtggtgcttg ttcaggatgc
     7081 tcctaccgat aggaaactca ccagtgacat ggaagccatg atcgacgggg tgaaggtcat
     7141 ctggcgaaag tcgtcacccc aatgagaaaa cgaatctaat accggatgag atagcctgaa
     7201 gaaatggaac atatcgccgt cgctatcatc tccactacaa caaggcgaag gcctttttgc
     7261 agcattgtat cggacgtaac taatacgcta aatacccccg caaatatggg attatgcagt
     7321 aatgcaaaag aggaaccagc cacaacgact cgttcctctg gctttttatg gtcagccacg
     7381 ggccaagacg gcccgcgggc atgaccaaat agagtacaat atcgaaccga cgccttttac
     7441 ctcttggaaa actgaggcgc ctttcgggcc ttcttcaggc cgtacttatg gcgttctttc
     7501 atgcgcgaat cacgcgtgag aagccctgct ttcttaagaa cgggcctcag ttcaggctcg
     7561 ccctcacaaa gcgccctggc tattccatga cgtatcgccc cagcctgccc gcttagcccg
     7621 ccacctgaca cgtcggcaat ggcatcatat ctgtccatcc tattcgcgac ctcaaacggc
     7681 tcgcgtgcaa gcaactccag ggtctggcgt ccaaaatact cctcacttgg tttgccgttg
     7741 accgtgatct tcccggttcc cggcgtcagc cgcacacggg caactgagca cttgcgcctt
     7801 cccgtcgcgg catactgcaa ttgcgccaca gagtaatccc ccttcctcgc ccggttatac
     7861 cgttatttcg agcggctcag gcatctgcgc cgcatgcggg tgttccggtc ccgcatagac
     7921 tttgagtttc ttagccatct gccgtccaag cctgttcttt ggaagcatcc caacgatcgc
     7981 tttctccacc gctttctcag gcttagtcga aagaaactct tcatagttca tctggcgaat
     8041 cccaccagga tatcccgtat gccaatagtg aatcttggtc tgacgcttgt tccccgtcag
     8101 gactacctta tcagcgttga tgactatcac atgatcgccc atgtccatat gaggagagta
     8161 gaccggctta tgcttgccct tgagtatctg ggccacttcg cttgcaagcc tgcccaaggg
     8221 ctttccctcg gcatccacaa cataccactt gcgcgatatc tcctccgcct taggcgtgta
     8281 tgtgcgtgac atcaggttcc ctcccagctt caactgctcg tctgagtgca atcagtggtt
     8341 ccggggtttg gcgccactgg tacaaaactc accaagcaat tttaatatag ccaaaataga
     8401 ctgtcaaggc gaggcgagac cacctatggg ttctgggtag gctactttca tcaagtagag
     8461 tccgtccgcc ggagcggtcg caggcccgag ttttcgatct cgcaattcaa gcaatcttcc
     8521 cggaccctcc gaatccattc tgcccatccc aacctccaaa agcgctccta ctatagtacg
     8581 cgccatcttg tataagaacc catccgcctc gatctccaca accacgagat tcaacccgag
     8641 agattcccat ctgtcggtat taacctcaag tcgatgaata gtgcgcacag aagtccttgc
     8701 tgaggacccg gatgcagcat aagccgagaa atcgtgacgc cccactagcg ccacaccggc
     8761 ctctgccatt gcctgcaccg cctcatcagt cagcggattc ctcgtaagca atgccctttt
     8821 cgagagcaag gcgccaccgc tgcgcgctgc cggaaccctc gccgcttccg gcacaatcaa
     8881 gtacctgtaa atcttccatt ccgcatcata acgcgcgttg aaatcttctg gagcctcgaa
     8941 ggatcttatg atccgaatat cctcaggcaa cacactgtta agcgctgcgc caaacctatc
     9001 agcagggatt cttgattcgg ttttgaagtt tgctacctgc cctagggcat ggactcctgc
     9061 gtctgttcgg cccgccccaa ctatccgcac actctcgcct gtgagccgat gtattgcccg
     9121 ctccacttcg ccctgcacgg tgcgaatcag ccgccctcca cgtccttcga gtcctttccg
     9181 gctttcgccc cctcgcccaa atgcaggttg aacttgaaat ccctgatacc cagtgcccac
     9241 atactccatt acaagaccga tattgcgcat ctcacaatgc caccgccaca gccaccgtgt
     9301 acgcgccgac aactaccagc acggccacgt cagctccact gaaagccaat tcgcgcaagc
     9361 gggtccgccc ttcgccgccc ctatagcacc tggcttccat agccaccgcc aactcgtcag
     9421 ctctgcgaaa agcgctcacg aagagtggca caagtatggg caccatcgcc ctcgccctct
     9481 tcgcaatgtt gcccgattcg aagtcggccc ccctcgccat ctgcgccttc attatcttgt
     9541 cggtctcttc cagcaatgtt ggtatgaagc gcaatgcgat agtcatcatc attgccagct
     9601 cgtgcgcggg caatccgaga cgcgcaaaag gcttcagaag acgctccagc ccatctgtca
     9661 aggcaatcgg ggatgtggtc agcgtcacaa tcgaagttgc catgatcaat aacgccagcc
     9721 gtacaacggt gatcaacccc agccgaagcc cttcatatgt tgctgtaatc ctcccgatgc
     9781 taaagatcgc ctcgcctgga tccatgaaca ggtgcaaggc aaaagtaagc accatgataa
     9841 acataagcgg tcgcaagctg cgggtgatca gtttaaaagg gacctgcgaa accatgattc
     9901 ccagcattat aggaagtgcg agcaacgcgt aggcccacgg ctcgctcaca acgaacagac
     9961 caatcacaac accgagcgaa gtcaacatct ttgtccgcgg atccagcctg tgcatgaagg
    10021 aatcccctgg catatactgt ccaactgccg cattagcgaa gtccatgccc acaccttcct
    10081 tcacatgggt ccgggcctgc agatccagtc gactcgatag acgccgcaag aagcggcggc
    10141 aacagaatct cacgcgcttc ctcaaccgtg attgcaccag tcgagaatcc atatccccgc
    10201 ctggctagct cggccatgag gtcagtgatc tgaggaaccc ccaagcctat gtcgatcagc
    10261 tcctgacggt gcctgaatac ctcgcgggga tcaccgtcca tgactacctt gccggcttcc
    10321 atgaccacga gcctctttgc gaatcttgca acatcctcca tgctatggga gacgaggact
    10381 atggtgtatc catgcttttc atgaagcgcc tggatctctc cgagaacatc ctccctgcca
    10441 cgcggatcca tgccggcggt gggctcgtcg aggattaaca ccttcggaca catcgcgatc
    10501 acgccggcta tggccacacg cctcttctgc ccgccggata gctcaaaagg agatctgtca
    10561 gcgaactctt catagtcaag gtcgacaatg cgcatggcct cccgaacggc atcatcgacc
    10621 tgagcatcgg acaatcctag gtttttcggg gcaaacgcga tatctaaagc aacggtctcc
    10681 tcgaagagct ggtgctcggg atactgaaaa accaagccta cttttcttct aatatcccgc
    10741 agcgcaacac cttttcccca gatatcctgg ccatccacga gcacgcgccc tgaatcaggc
    10801 ttaaggaggc cgttaaaatg ctgcaccagg gtagatttgc cagagccggt cttgcctatg
    10861 atcgccacaa gatctccttc gtcgatcgac agattgacgt ctgtaagggc ctgacgcgcc
    10921 aatggcgtgc ctcgcatata cgtatagcat aggttctgaa cttcaatgat tttggagctc
    10981 actgctgcac ggccccctgc ccaaaagagt tccaatgtcg tccgccattt cctcaaccgt
    11041 cagcggatac cttggaagcg ccacacctgc cgctcgcagc tgggcagcga gttcagtaac
    11101 ttgtgggaca tccaagtcca gcttgcgcaa tgtggccggc tgcgagaaga cctctcgtgg
    11161 cgtcccggag agagcaaccc gcccctcttc cattacgata acacgatctg cataaacagc
    11221 ttcgctcatg aagtgtgtta tatgcacaat cgcaattccc tcattggaag cgagatggcg
    11281 cactgtacgc agtacttcac ttcggcccac cggatccagc atcgcagtgg gctcgtcaag
    11341 gacaatgcac ctgggtctca tcgcaatcac gcctgctata gccacccgct gcttctgacc
    11401 tccggaaagc atgtgggggg cggatttctc atacccagtc atgtccacaa gcgccagcgc
    11461 ttcatctaca cgccggcgca tttcatcagg cggaactcca aggttctcgg gtccaaacgc
    11521 cacgtcctct tcgacgatgg tcgccactat ctggttgtca gggttctgaa acaccatacc
    11581 aacagtctga cgtatatccc acaacaacga ttcgtcgcga gtgttcatcc cgagcacgta
    11641 gacgtcgcca cgtgttggaa ggagcaaggc gttcatatgc ttagccagtg tcgacttgcc
    11701 tgatccgttg tgtccgatta tggccacaaa ctcgccaggc caaatctcca ggctaacgtc
    11761 gagaagcgcc tctacctgtc cttggcttcc gccatcatag gcatagctca cattctcgac
    11821 gcgcactacg ggtttcataa cacatcactt cttggctttc gccggcttcg cgcttacgct
    11881 gctgcccggc atatctacca gctcaataat ggctagcggc gctccatccc cacgcctttg
    11941 gccaagcttt ataatgcgag tatacccgcc cggtctatca atgttcgcag gcccgatctc
    12001 atcaaacaat ctttgcagcg ctacaggatc gagtacatac ttggctgcaa gacgcctgtg
    12061 atgaaggtcg ccaacctttg cccatgttat gagtctttca gcaactgggc gtatctcttt
    12121 tgccttcgct tccgtggtga ttatcttgtc gtgtaaaaac agcgacgtca ccgagccccg
    12181 gaaaagagca cgccgctcct ccgctgtacg gctgagcttt ctgcggctca tctgcgctct
    12241 cccccctaca ctactcatca ggctgtcgga gcgacagtcc caatgattca agcttctgct
    12301 tgacctcttc aagcgatttg cgtcccagat ttctcacttt gagcatttcc tcttcagtct
    12361 tgtgggttaa ctcgtcgaca gtgtttatgc ccgctctctt caggcaattg tacgaccgca
    12421 ccgacaagtc caagtcttcg attaccattt caagggcccg atctcggcaa tcctcttcgg
    12481 gttcagcctc cggctcaacc tcttcctcag gctccgaaag gcttatgaac agcttcatgt
    12541 gttcattcat catcgaagcc gccatgctga cagcctcttt cggagtgatg gtcccgtgag
    12601 tccagacctc cagaaccagc ctatcgtagt cggtaactcc accgactcga gcattttcca
    12661 cggtgtagtt caccttttcc actggagtga agatagagtc cattggtatc acgcctatgg
    12721 gttgctcagg cctcttgttc ttctcggaag acacataacc ccggccgggt tccacgacgg
    12781 cttcaagcga cagaactgca cctcgctctt ccgtctttcc ggtatcctga ttcaccgcct
    12841 caactgtgcc caatgtggca atgtgcagct cgggattgag tatgtctatt tctgcatcgg
    12901 cctcgaaatc agcggccacg acatcaccgg gaccttcggc catgagccga atgaccttgg
    12961 gcacatctgt atgcagcctc acgagaagac tcttgaggtt caggatgata tctgtcacat
    13021 cctcataaac cccgggaata gtcgagaact cgtgcaacac cccgtcgatc ttgaccgagg
    13081 tgactgcggc cccgggcaat gacgacagaa ggatcctgcg caatgagttg cccagggtta
    13141 tgccgtatcc cctttcaaga ggctccacga caaacctacc gtatcggtcg ccatcctctt
    13201 ggcaatcgat tctaggcttt ggagattcaa tcatagcgcg ctcagcaccc tccttctcat
    13261 ttggggcagc attacttcga gtacaactcg acgattaagt gttcctggat atccaggtct
    13321 atctgatccc ttgtcggcac tgacagaact ctgcctccca aacggtcctc gtttactgaa
    13381 agccatgcgg gcacagtcac agcgcctgct tcggcaagat tcttgaagtg gacaaggttc
    13441 ttgcttcccg gtctcacaga tatctcatcg cccgcacgaa gctcatacga tggtatggac
    13501 accctcttgc cattcacgta aatatgcccg tgcctcacca attgcctggc ctctgcccta
    13561 gaggcaccca gtcccagtct atagacgaca ttgtcgagcc gggtctcaag gatagcgagg
    13621 aggttctcgc ctgtaatgcc ccgtctgcgc tcggccatat caaaatacct tgcaaactgc
    13681 gcttcctgca ctccgtatat ccttcgaagc ttttgcttct cgcgaagctg caaaccatac
    13741 tcgctggtcc tctttcttcc ctgaccatgc tctcccggcg ggtaagccct ccgggcaaca
    13801 gcacacttct cagtatagca tctatctccc ttgaggaaga gcttagctcc ctcacgtcgg
    13861 catagccggc acactgatcc ggtatatctc gccattatcg cacctcctgt tcgctcggct
    13921 acacgcgccg tctctttggt ggcctgcatc cgttatgcgg aatcggcgta acatccttta
    13981 tcatgctaac ttcaagcccg gcagcctgga gcgaccttac cgcagcctct cgtcctcctc
    14041 cggggccttt taccaacact tcaactgaac gcattccctg atcaatggca ccacgcgccg
    14101 cggcgtccgc agcctgttgc gctgcaaacg gcgtaccctt acgtgagccc ttgaagccca
    14161 tggcgccagc ggatgaccac gacacaacag ctcctgccgg atcggtaatg gtaacaatcg
    14221 tattgttgaa gctcgacttg atatgggctg ttcctgtttc gatattcttc cgttcgcgcc
    14281 ttctaggcct tgcctgcctc tttgccatca gtgttcctcc tttccgcaac gcgccatgct
    14341 aatcaccttt ttttgcgccc gccaatggcc ttcttcgggc ccttggccgt tcgagcattc
    14401 gtcttcgtcc tctggcccct tacgggaagt ccgcgacgat gcctcagccc tctgtaagag
    14461 cctatatcga caagcctctt aacattctgg gcttcttcac ggcgcaggtc tccctctacc
    14521 ttatagcctc ggtcaatagc ctctcgcaat tgcgtgatct cggtctcagt aagatctctc
    14581 acccgcgtat cagggttaac gcccgtctcc gccaggatct tctgggatga ggtgaggcca
    14641 atgccgtata tataagtcag ggctacctct atccgcttgt ctcttggaag gtctacacct
    14701 gcgattcgcg ccatgctacg ctgcacctcc tagccctgtc gctgtttatg cgtggggttc
    14761 tcgcaaataa ctctaacgcg gccttttctc ttcactatct tgcatttttc acacattttc
    14821 ttaacggacg gccgtacctt catgtctaac cctcctactt gtatctccac acgatcctgc
    14881 cgcgagtcag atcgtaaggt gatagctgaa tcgtgactct gtcacctggc agaatcctga
    14941 tgaacttcat gcgcatcttg ccggacacgt gcgccaatac cgtatggcca ttttccagtt
    15001 ccaccttgaa catggcgttt ggcaatggtt caacgactgt gccctcaact tctatggcat
    15061 cctcttttgc cataactaag catccccctc cttcctctct tgaggtctgt acactctcaa
    15121 agccgtaaca agttgttcat cggtgaccga gtttccggcc tcgagcgcgg cccttatatc
    15181 ttcatccacc tgctccgtca ctatcaggtg cttaacgttt ttccgcttcg ccttgcccaa
    15241 cttgtgagtt gcgccgtcag caatgtaaac gtaaccgtca ccttcacggc cgactacgac
    15301 aaagagcctg cccctgtccc tgcccatctt agatactacg aactgtccaa gcaatggacc
    15361 cccggaagac aactgcctta cacctgcctt gcatgctacg gaagagtcaa aacctcgggg
    15421 ccagcatcag ttatggctac cgtgtgctca aagtgggccg acagcgatcc atcacgcgtg
    15481 cacgtagtcc agccatcgtc catgatcatt acttcgtggc cgcccgcatt caccatgggc
    15541 tcaatggcaa ggaccattcc aggcctgagc cgtagtcccg tgcccggcga cccaaagttt
    15601 gggatttgag ggtcctcatg catgcgccgt ccgatgccgt ggccaacaaa atccctgacc
    15661 actgaaaacc cattactctc ggcatgtacc tggatggcat tgccaatatc cccaagacgc
    15721 atcgtcggcc ttgccatctc tattccagcc tcaagcgcct cccgcgtcac tcgcatgagc
    15781 ttctccgctt caactgagac atctcccact ggaaaggtca tggctgcatc cccctgatac
    15841 ccgtcgagga ttgcaccgca atcgatagat actatatcgc cctcaaccag cacccgtttg
    15901 ccaggtattc cgtggatgat ctcgtcgttc acagacacac agatcgatgc cgggtatcca
    15961 cagtaaccct tgaacgacgg cgtcccgcct gcggccagta tcatttcttc ggcaatggca
    16021 tcgagctccg ccgttcggac acctggggcc acgtatctgc ccagtctctc cagcacctgt
    16081 ccgaccaggg cgcccgcttt ccgcatctgc tcgatttcag ccgcggtttt cagaatgatc
    16141 atgacccaca ccttccaagg gcacgtgtta tgcgcctgaa aacctcatca ggatctcccg
    16201 ttccatctac cacgacgagg cttccctcgg aggcataata atccctcagc ggttctgtct
    16261 gcgttcgata cgttgcaagc cgctccttca ctgtctccgg aaggtcatcc ttccgttgag
    16321 tgaccagacc accacactcg cacgcggatc ctggaggcgg attccattcc atgtggtaca
    16381 cacgaccaca cttctcgcat actctccgtc cggttaccct tcgcaccaag acgtcctcgg
    16441 gaacctctat gagcaccact gcatcaagcc ttacgccctg ggctcgaagt atgtcttcaa
    16501 gtccctctgc ttgcggcaca gtccgaggaa atccgtcaag aataaatccc gacattgcat
    16561 ccgcttcggc caacctctcg caaacgatgc caagagtcac gtcgtcagga accagcttgc
    16621 cggcttctat gaatccctgt gcccgccgac caagctcggc gcctgccgct atcgcggccc
    16681 tgaacatgtc ccctgtggat atatgcggga cgccgaactc tgctgccagc ctgacagctt
    16741 gagtgccctt ccccgcgctg ggaggtccca tcagcactgc acgcatctcg cgacgcactc
    16801 ccatcctcat tttctttggc attccaaagc cttacttcaa gaagccctcg tactgcctca
    16861 ttatcagctg agcctcgatc tgtttcatcg tgtcaagcgc aacgcctaca acaatcagaa
    16921 gcccagtacc gccaaacgtc acagtgttag atgtaagccg tccggttatg ttaggcaaaa
    16981 gcgctatgat agccaggaat attgcgccca cgaaggtgag cctcgtcaac acgcggtcga
    17041 gatactccgc cgtcggccgt cccggcctga acccggggat aaatcccccg tattttctca
    17101 tgttatctgc cacttccacc gggttaaatg tgatgcccgt atagaaatag ctaaagagaa
    17161 ttatcaaagc aacgtatacg atgttgtaag cccatacgtt gttcatgaac gccctgacac
    17221 caggtgccat cccaggtatg aattgagcca acgtgccggg aaacgctaac accgatgacg
    17281 cgaagatcac gggtataaca cccgcctggt tcactttgat cggtatatga gtgctctgcc
    17341 cgccgtaaac ccgccggcct acaaccctct tgggatactg cacaggcacc ctgcgctgcc
    17401 cttcgctaat tgccactacg ccaattacaa gcgccaggct catcagcacg tatacaatcg
    17461 caaacaccca acctgtcccg cccgacttca ccaggctaat gacgttttta acattgcctg
    17521 gcagctgcgt gacaatgccg gcaaaaatga tcagagaaat cccattccct attcctttct
    17581 cggatatctt ttcgcccagc cagatgacga acgaggcacc ggcagtcata ctgacaatca
    17641 cgagcaaggt ccagactgcc ccggggcgga tcgcctccct gaacattgcc gaatatccgt
    17701 atgactgtaa aaccgccagg gcaaccgcga ggtaccttgt atactgcgaa attttcttcc
    17761 gtccctccgg accctccttg gacaattcct caagcgacgg aatgacaact gtcagaagtt
    17821 ctatcacgat cgacgcggta atataggatc ccacgttgac cgcaaacact gacagcctct
    17881 gtatactgcc tcctgaaaac atgtcgacaa ggcccagaag gccgccgcct gtctcgccca
    17941 ttaccgcctg aaacacctct gcgaccttgg ccatatcgac acctggcgcc ggtatgaagg
    18001 caccaagtct gtacacgaac aacaggaatg cgctgtacag gatcttcttg cgaagatccg
    18061 ggaccctcca agcatttacg agcgctgcca gcacctatac cacctccgcg gaccctcccg
    18121 ccgccttgat cttcgacatt gcgctctcac tgaacttgtc agcgcgaacg gtgagggact
    18181 tcgtaagctc tccgtctcca agaaccttaa caagcgcgtc atgacctttc acaaggccct
    18241 tttcgcggag cgcatccctg tcaacctcat caccagcctc aaaaacccgc tcgaggtcgg
    18301 aaacgttaac gactgcgtat tcaaccttat acttgttggt gaagccccgc ttcggcagct
    18361 gcctgtaaag cggtgtctgc ccaccttcaa acccagggcc cttgcctccg cctgagcgag
    18421 acagctggcc cttgctaccc cggccggcag tcttgccgtg cccggaaccc gtaccccggc
    18481 ctactctctt gggggcgcgc ttcgcgccgg ccgcgggctg tagttcatgc agtttcattg
    18541 ctgtacccct ctcagtcgat ctcagtaact tcgaccagat gacttacttt gttgatcatg
    18601 cccctgataa caggggtgtc gtcgtgttca accgtctggc caatcttgcg gaggcccaga
    18661 gagcgcacag tcgcccgctg gtctttgggt ctgctgatgg tgcttctctt caacgtgaca
    18721 accagcttct ttgccatctt tcgacccctc ctacctagca agttcctcga cagacttgcc
    18781 gcgtgtccga gccacctgct ccgcggtgcg catggactgc aagccaacca aggtagcatt
    18841 gaccatgctc attgggttat tcgacccaag agatttcgtg agtatgtcgc gataacccgc
    18901 aagttcgaga accgccctaa cagcgcctcc agctatgact cccgttccag ggccagcggg
    18961 tttaagcata acacatccgg cgccggattt cgcggtcact tcatgcggaa cagtagttcc
    19021 caccataggg acggacacca agttcttctt ggcatcgtcg actccttttt ttatggcctc
    19081 cgaaacttcg taggccttcc caagcccagc cccaacatgc ccgttttcgt cacccacaac
    19141 taccagcgcc cgaaagctcc gcgttcggcc gcctttagtc gtcttggcca cggggttgat
    19201 gctaaccact ctttccttaa gatccagact atcaggattg atcttagcca tttactgccc
    19261 tcccccgcct caaaactcta agccgccctt gcgcgcggcc tcagccaatg cagctaccct
    19321 gccatggtaa cgattgccgc cacggtcgaa tacgacctta cggattccct tctctatggc
    19381 tcgctccgcc gctactctgc ccacttcctc cgccgcggca atgtttccac cataccctac
    19441 ctttgccctg atgtccggct ccacagaaga cgccgccacc agggtgtggc ctgtcgtatc
    19501 gtctattatc tgaacatata tgtgggcatg gctccgaaac acagacagcc tagggcgttc
    19561 gggggttcca ctaacatcct tccgaacgcg ctgatgacgg cgggccaggg cctctttcct
    19621 gtcaacccta ttgtacattg cacgtcgctc ctttcattct gccgcaagct gcagcatcag
    19681 ccgatcttgc cggccttgcc agccttcagc gcaacgcgct cgcccgcgta tcttatgccc
    19741 ttgccatggt atacttcggg ctctctgaca gcccttatgt tcgcagcagt ctgcccgacc
    19801 aactgcttat ctgtgcccct gactgttatc ttcgtcggag acggaacgtc tatctgaata
    19861 ccgttttccg ggactatctc cacaggatgc gagtagccga cttgcagcac caacgtttcg
    19921 ccttgcttgg atgccctgta tcccgtccct atgatctcga gggatttttc aaacccctct
    19981 gtaaccccat gcaccatgtt ggcgatgagc gcccgggtca ggccgtgcat agatctgtca
    20041 agtcgattat cggatggacg cgtaaccctg atctcgccgt cagccacctc aacgctgatc
    20101 ctcgcaggga tctcctgaga cagagcgccc ttggggccct ttacagtgat tatgctgcca
    20161 tccagttcaa cctctacacc cttgggaacc ggcactggtt tcttgcctat tctagacatg
    20221 tctacaccac ctaccacatg tagcagatga cctctccgcc aagcccggct tccctcgcct
    20281 gacggtctgt catcagccca cgagatgtgg agattacggc cgttcccagg ccgttcagga
    20341 ccttcggaac ctcatccttc ttaacgtaca ccctgaggcc gggcttgctt atccgcttga
    20401 ggccgtgtat taccttctgc ttgttaggcc catacttcat gtggacctta ataacatcgt
    20461 acttgtctcc ccgagtgacc tcaaagccgc ggatgtagcc ctcatctttc attatctgag
    20521 caagctctgc cttcatccga gatgctggta tctcaacgac ctcgtggttc actgtattcg
    20581 cgttgcgtat tctagtaagc atatccgcga tcgggtctgt caacaccatc gtcctctacc
    20641 tcctctcgtg gcgtgcccct gctgtcacca actcgccttg gtcacgcccg ggatttctcc
    20701 cttcgaggcg agagttcgga agcagatcct gcacattttg aacttgcgca tatatcctct
    20761 cggtcgcccg caaatcgagc accggttata ctgcctcacc ttgtatttcg gctctttctg
    20821 ccacttctct acaagcgcct ttcgcgccat atcttcccct ccttactgca gcctgactgg
    20881 caggcccata agcttcaaca gctcgaaagc ttcttcatcc gtttttgcag tagtcacgat
    20941 ggttatgtcc atgccacgga tcttgtcaac atcgtcatac cggatctccg ggaaaaccaa
    21001 ctgctcccta agacccaagg tatagtttcc ccgcccgtca aaagcgtccg gagaaacccc
    21061 tctgaagtcc ctaatcctcg gaagagcgat attgaacagc ttatccagaa aataatacat
    21121 ccttgcgccg cggagggtta ccttgcaccc aatgggaact ccggcccgca gtttgaacgc
    21181 tgcgatcgac ttcttcgccc gggtgattat cggacgctgt ccggtaatat tggctagttc
    21241 gtcggcggcg gcgtcaagcg ccttggggtc cacaatggcg tctgatacgc ccctgttgac
    21301 gatgaccttt tcgacctttg gaacctccat gacgttcctg tattcgaact tctgcataag
    21361 cgcagggaca acctcgttct ggtatttctc cttcagccgt ggagtcggcg cctccatata
    21421 attgaccccc tctcaggcct atcgactatc ctacttttta tccagcttgg ctccgcactt
    21481 cttgcatact ctcatgggct tgccagactc cccaagttcc atgccaaccc gcgtcggctt
    21541 gttgcacttg ccgcagacca ccatgactct gcctagcgcc atgggcgcgg gtttgtcgat
    21601 gatgcctccc tgcggattcg cggtgctcgg acgagtatgc cgctttacaa tgttcaagtt
    21661 ctcaacgata accttctgtt cacgaggcag caccctgagg accttgcctc tcctgcctgc
    21721 atctttgcct tggagcacca taacggtgtc gcccttcttg accttcttca cggatatcgt
    21781 cattttatcc acacctcctc gctcctgggg accgccccgt agcctagagg acctccggag
    21841 caagcgatat gatcttcatg aaattccgat cacgaagctc tcgcgccaca ggcccgaaga
    21901 ttcgcgtgcc ccgtgggtta ttattggcgt cgataacgac cgccgcgttt tcatcgaatc
    21961 tgatatatgt gccgtcggcc cgcctgagct ccttcttggt gcgaacgaca acggccttga
    22021 cgatatcgcc cttcttgaca acgcccccgg gcgtggcctc tttgacagtg ccaataatga
    22081 tgtcgccaat accggcatac cggcggtccc cgccgcccaa gacgcgaaag cacatcactt
    22141 tcttggcgcc tgtattgtcc gctatattca gtacggtctg ggcctgaatc acagagtgtt
    22201 cctcccttca atagggccct tgttacttag ccttctctac tatctttaca agcctccaac
    22261 gcttatcgcg tgagatcggc cgggtctcca tgatgagaac cttgtcacct actctgctct
    22321 cattgccctc atcgtgcacc ttaaccttgc tggaccgacg caccactttg ccatacatcg
    22381 gatctttgta ggtgcgctgg agttccacca ctcgggtctt gtccattgca tcactgacta
    22441 caaacccgac tctctccttg cgctcatttc tagcagtctc catgacctgg cctcctctct
    22501 accctcgtgc aatcttgagc tcgcgttccc gcatttccgt cttcaccctg gcgatatcac
    22561 gcctaacatc ctttatccga tgcgcgttgt ccagctggtt tgtggcgagc tgaaacctga
    22621 ggttgaagag ttcctccttg aggccttcca gcctagtatc gagttgttcg ggggtcatct
    22681 ctctcagctc tctagccttc atcggcttca ccacccgctt cttctctctt tacgaactgc
    22741 gtcttgatcg gcagcttatg ccctgccaat ctcattgctt cacgcgcaat ctcttcggat
    22801 actccgccca tttcaaacat tactcttccg ggcttcacca ctgccaccca gtactccggc
    22861 gcacccttgc cgctccccat acgtgtttcg gccggcttct tgctcacagg cttgtctgga
    22921 aatatcttta tccacacctt gccgccacgc ctgatgtatc tggtcatcgc aatacgggcc
    22981 gcctctattt gtcgcgacgt tatccatcca ggctcaagcg cctgaaggcc atactcgccg
    23041 taggtgacgg tggttccctt ttcagcaacg cccttcatgc gtcccctctg ctgctttcgg
    23101 tacttgacct tcttgggcat cagcatttat cgagcccctc ccccctgccg agctttcccc
    23161 gacttatctg ggagaacatc acctttatag atccatacct tcactccgat tttgccgtac
    23221 gtcgtatttg cctcagtgaa cccataatca atgtcggctc tcaacgtgtg aaggggaagc
    23281 ttgccataac tgtaaccttc cgtcctggct atctctgcgc cgccaaggcg accgctggat
    23341 ttcacccgca ttccctgaac cccaagccgc tcggctctgc ccatggcctg cttcatcgca
    23401 cgtctgaagg agattctcct ctccagctga gctgcgatgt tctccgccac caactgagca
    23461 tcgagttcag gatgcttgat ctccataatg ttcaccgaaa cctgcttgcc cgtcatggcc
    23521 tccaggtccc tgcgaagcgc atctgcttcc gaaccgccgc gcccaataac cataccagga
    23581 cgggcagtgt ggatggtgac tctaatcctg ttcgccgcgc gctcgatctg tatccgcgaa
    23641 acgctggcgg cataaagtcg gttcttaatg tacttcctga gcttcaaatc ttcatggaga
    23701 agctcggcat agttcttatc tgaataccac gtagactccc aggtccggtt gatgccaagc
    23761 cgcagaccta taggattgac tttctgcccc acactctaac cctccctttc tctgaccact
    23821 accgttatgt ggctcgtgcg cttgaggatc ctgtttgcca tgccccttgc gcgcggccgt
    23881 atccgcttga gcgtagggcc ttcgtcaaca taagcctcag cgatatagag gtcatccctg
    23941 ctaaggtcga ggttgttttc ggcatttgcc gctgcagaag caacaacctt gctgaccggc
    24001 tccgccgcaa tgtgcggagt atatttaaga atggtcaggg cttcatcaac acttttgccg
    24061 cgcacaagat ccacaaccct gcgaaccttc tgtggcgaca tgcgcacata acgcgctacc
    24121 gctttcgctt ccacctgcgc ccgacctcct acctcttgat ctgtgtagtc ttatccgatt
    24181 gcctatggcc cctaaacgtc ctcgttggcg caaactctcc gagcctatgc cctaccatgt
    24241 cctcaacgat gtatatcgga acatgcctgc gtccatcatg cacagctatg gtatggccaa
    24301 gcatgtctgg ggtaatcatt gacgctctgg accacgtctt tataatctct ttttttcccg
    24361 ctgaatttag cctttcgacc ttctcagcta aggcctcgtg gacatacggt cccttcttca
    24421 acgacctgcc catatattgg cgcgcctcct ttcagaaccc acttcttact tcttgccccg
    24481 gcgacggact atcaacctgt cagaagcctt ggccttgcgc gtcctgtatc ccagtgtcgg
    24541 aactccccaa ggtgtgaccg ggctcttcaa cccaataggc gacctaccct cgccgccacc
    24601 gtgcgggtga tccaccgggt tcatcgcgac tccacgaaca ctcggccttc tccccatcca
    24661 tcggctgcgt ccggcctttc ccatagagat gttctcatgc tccacgtttc caacctggcc
    24721 cacggtagct cggcactcct ggcgaaccat cctcatctcc gtcgaaggca agcgaagagt
    24781 gacatactcg ccttctctcg ccattatctg agcgctggcc cctgcagatc tggcgagctg
    24841 cgcgcctctt ccggccgtaa gctcgactgc atggactact gtgcccacag ggatgttgga
    24901 aagcggcaat gcgttgccgg gcttgatgtc ggcatcaggg cccgactcta taacgtcgcc
    24961 aaccttgagc ccaagagggg ccaagatgta ccgtttttcg ccatctgcgt agttgactag
    25021 tgcaattcgc gcagatcggt tcgggtcata ctctatggac gcgattctgc caggaacccc
    25081 gtccttgtca cgcttgaaat ccacgactct gtatgccctc ttgtgtccgc cgcccctgaa
    25141 ccgaacggtg gtcctgcctt gggcattccg tcctccgctc ttcttgagcg gaaccaccag
    25201 cgacttttca ggcttcgagg acgtgatctc ctcaaacgtg gcaaccgtca tctgccgccg
    25261 tgccggcgta gtaggcttaa aacttttgac tcccatcccg catacctcct ttacgtgaac
    25321 ttagacccct tcgaagagcg ggatggtctc cccgtcctgc aaggtaacga tggccttttt
    25381 ccaggctggg gtcattccct ccgaacgtcc ctgtctcttc agctttccgg gaacatttat
    25441 ggtgtttact gcaactaccc gaacgtcgaa tatcttttcg acagccctgg cgatctctat
    25501 cttgttagcg tcacgcttaa ccttgaaaac gtattttccc tctgccgaca gagcgttgct
    25561 cttctcagtg atgatcggcc ctatgataat gtcccgcggg tgtgccatca cgcaaacgcc
    25621 tcctccgccc ttgaaaccgc atcgcgcgta agtaccagcg ctgtgtggtt cacaatatcg
    25681 tagacattta ggccttcaac atggagaacc attgcccccg gaatattccg agtggacttc
    25741 tccacaacct cgctccgctc gtcagtaaca acaaggggct tttcagcgcc tattttctca
    25801 aaaagcgctg ccatgctctt cgtgcttggt tcggatagat caatggcgtc aacaacgtat
    25861 accgcgttct cctgcgccat cgatgacagc gccgacaaca gcgccaacct gcgaaccttc
    25921 ttcggaatcg tgtaaccata ctttctaggc ttagggccga acactactcc gcccccaacc
    25981 catatgggcg acctgcgcga tccgtgtctc gcccttccgg tccccttctg gcgccacggc
    26041 tttcgaccgc cgccccggac ctcggagatg gtcttcgtgc tatgcgtgcc cgaccttctg
    26101 ctggcaagct gcagcgtaac cgcttcatgc actgcgtgct cgttgggctc tattccgaag
    26161 acggcttcag gaagctctac ctctccgagc ctattgcctc ttgcatcgaa caatggtgct
    26221 ttgggcatct cctgcgcctc cttactttgc cttcgccttt acagagtccc ggatagtcac
    26281 aagaccatta gtggcaccgg ggattgaacc ccgcaccatg agcaggttcc tctccgggtc
    26341 gacctttacg acttctagcc ccaggctcgt agctttcgtg ccaccgagcc tgcccgccat
    26401 cttgcgtcct ctaaaaaccc gagccggagc ggtcgcaccc agtgatccaa ctccccgatg
    26461 atagtgggaa ccgtgcgcca tcgggccgcg gctgaaattc cacctcttca tcggtccggc
    26521 aaaccccttg ccccgagaga tgccagtcac gtctacaagg tcgcccactg accactgatc
    26581 gaccttgatc tcctggccaa gctcataggc atccgctgac tctatgcgaa actctttcag
    26641 atgtctgcta ggcttcacat tcgccttagc ataatggccg gccataggct tgctcacacg
    26701 ccgcttgtcg atttcgccga agccaagctg aaccgcttcg tagccatcgg tggcggtgtg
    26761 cttcttttga acaacgcaac atggccctgc ctctataact gtaaccggat gaaccctgcc
    26821 gtcttcttcg aaaatctggg tcatcccaat ctttctgccg attatagctc tgcccacgcg
    26881 tcacaccccc tcgtggccta caacttgatc tctatgtcca ctcccgccgg aaggtcgaga
    26941 cgcatcagag cgtcgatcgt cttgggagta ggatctagga tatcaatcaa cctcttgtga
    27001 gtcctcatct cgaactgttc gcgagaatcc ttgttcacgt gaacagaacg aagcactgtg
    27061 tatacgtttc tctccgtcgg gagcgggata ggccctgaga cctctgaccc cattctctta
    27121 gcagtctcca caattctctc ggcggactga tccaatatct tgtgatcaaa agctttcagc
    27181 ttgatccgga ttttctgagt tgccaaattg gctccctcct attcgcccga ttcctttgac
    27241 ggacatactc cacggaagtt actccggcag cttcccgcac agacaatctc ccgcttcatc
    27301 gcagattggt gggggcggga cttcccgccc ccacgttgcc gcattattca ctgtccgaac
    27361 tgcggtcggc ttactcgata atggaagcca ctgctcccgc gccaacagtg cggccgccct
    27421 cgcggatagc aaaacggagc ccaggctcca tggcgattgg agtaatgaga ctgattgtca
    27481 tcaccgtgtt gtcgccaggc atcaccatct ctgtgccctc aggcagctgt acgtttccgg
    27541 taacatccgt tgttctgaag taaaactgcg gacgatagcc gttgaagaac ggagtatgcc
    27601 taccgccctc ctccttagtc agaacgtaaa cctgagcagt gaactttgta tgaggagtga
    27661 tggaccctgg cttcgccagc acctggccgc gctctacctc atctctgtca atacctcgaa
    27721 ggagacatcc aacgttgtcg cctgcaacac cctggtcaag aagcttgcgg aacatctcta
    27781 cgcctgtgac cactgtcttc ctcggctgag gcatcaaacc gacgatctgc acctcgtctc
    27841 cgaccttgac aactccacgc tcgactcggc ccgtaacaac ggtgccgcgc ccagtaatcg
    27901 tgaaaacatc ctcgatcggc atgaggaacg gcttatcgat ttcacgctgc ggctctggga
    27961 aataggcatc gatagcttca acgagcttcc aaatcggacc gcaccactgg caatcctttt
    28021 gtccgcaccc gcactcgccg gccttgtacg ccgacagggg aattacagga atctcgtctc
    28081 ctgggaactc atactcgctg agcaaatccc ttacctctag ttcaaccagc tcaaggagtt
    28141 ccgggtcatc gaccatgtcc accttgttca gcgcaaccac aattgaggga acgccgacct
    28201 gtcgcgcaag caggatgtgc tcacgggtct ggggcatcgg gccgtctgcg gccgacacca
    28261 cgagaatggc cccgtccatc tgagctgcac cggtaatcat gttcttgatg tagtccgcgt
    28321 gtccggggca gtcaacgtgg gcatagtgac ggtttggcgt ctcatactcc acgtgggacg
    28381 tgctgatggt gattccacga gccttctcct cgggcgcctt gtcgatcttg tcaaagtcca
    28441 tgaactgggc aagcccattc ttcgccagga cattcgtgag cgccgacgtc agcgtcgttt
    28501 tgccgtggtc gacgtgccct attgtcccga tgttaaggtg gggcttggtc ctttcaaact
    28561 tctgtttggc catttctttc aatcctccct tgcattctca ggacaaaact cctcggtatc
    28621 gtcgcatgat ctccgcagcc aagttgtcgg ggacctgctc atacctgaga aaatgcattg
    28681 tatggttagc ccttccctgg gaaagtgacc tcatggttgt ggcataacca aacatttccg
    28741 cgagcggaac ctgcgccctt atgacttggt tctggccgac gttctccagg gcagtgacct
    28801 tgccccttcg cgcgccgaga tccgccatga tgtcaccgac gtaaagctcg ggcgtttcta
    28861 cctctacttt cataatcggt tccagaagca ctggtttggc acgttccatg gcttctttga
    28921 aagccaaaga ccctgcaatc ttgaacgaaa gctcagacga atcgacctcg tggtaagagc
    28981 catccacaag cgctaccgcc acgtcaacga ctgggtatcc tgccaagact ccattgcctg
    29041 ctgcctccat gataccggct tcgactgcgg gtatgaagtc cttaggaatc gctccgccta
    29101 caaccttgtt ttcaaagaca atgcctgatc cttgggcgcc cggttccagt tccaaaacga
    29161 cgtggccgta ctgtccgcgt ccacccgttt gccttacaaa tcttccttcg tgtctcaccg
    29221 gacacgttat tgtttccctg tatgcaacct gaggtttccc aacattcgcc tgcacgttaa
    29281 actcacggag aagcctatcg actatgacct cgagatggag ctcgcccatt cccgagatga
    29341 gcgtctgacc cgtctccgga tcggttcgaa ctcggaatgt gggatcctcc tctgcaagtt
    29401 tcgctaggga aactgtcaaa cgatcctcat cattcttgct ctttggttcg accgcaaccg
    29461 ctatcacggg ctcagggaac tccatgcgct ccaacacaac cggtgcacgc tcgtcacaga
    29521 gggtatctcc ggtggttgtg tttctcaatc ccaccgccgc aacgatgtca cctgtggata
    29581 cgagatcaag ttcctccctg tgattggcat gcattcttag tatgcgaccg aatctctcgc
    29641 gttttccctt cgaggcattg tagatcgcat ctccaacctt cgcaacccct gaatagaccc
    29701 gaaagtaggc aagcttgccc acgtatgggt cggtcgcgat cttaaacgca agcccacaga
    29761 agggctcgtc atcagatggc tttcgctcaa tctgttcttc atcgttggtc ggatctactc
    29821 cgactatggg cggtacgtcc atcggcgacg gaagatagtc gacaatcgca tcgagaagcg
    29881 attgcacgcc cttgttccta tatgcagacc cacatagaac cggagtcagt ttagccgata
    29941 acgtcgccct acgcaacccg tcaatgatgt cttgtgaagt tatctcttcc ccatcaacat
    30001 agagcatcat gacatgatca tcgttctccg ccaccagctc caccaagcgc tccctagcct
    30061 cagctgcagg ctggcgaagc tcttcaggga tctcgacaac ctgccgattc aagccgagat
    30121 catccatata gacgatggct ttttgcgata tcaaatcgat cataccggca aaccggtcct
    30181 cagcccctat tgggatctgc actggcgctg gcgtggcccc gagtctctca cgaatcgcac
    30241 gaacagccat gtcaaaatcg gcgcctactc ggtccatttt gttcacaaaa gctatccgtg
    30301 gaacccgata tcggtcagcc tgtcgccaaa ccgtctccga ctggggttga actccttcgg
    30361 ccgcgtcgaa gagagccacc gccccatcga gcactctgag agatcgttcg acctccacag
    30421 taaagtccac gtgcccgggc gtgtcgataa tgtttatttg ataatctcgc caatcgcacg
    30481 tcgtcgcagc agacgtaata gtaatgcccc gctcctgctc ctgcaccatc cagtccatcg
    30541 tggccgtgcc ggaatcaact tcaccgagcc ggtgaacccg accggtatag aagagaatac
    30601 gctcggtcgt agttgttttg ccagcgtcga tgtgcgccat aatgcctata tttctaattc
    30661 tctcaggtgg cgtcacccca agcatacctc tcccccctta cacgcgctgg atgccttgac
    30721 taccatctat aatgcgcaaa ggccttgttg gcttcggcca ttctgtgcat ttcctctttt
    30781 ctcttgactg aagccccggt gttctctgac gcgtcaagga tctccgcagc aagcctttcg
    30841 gccatagtcc tctccggtcg tttcctggca ttcccaacga tccatcggaa cgcgagagcc
    30901 ttccgcctct ccggtctgac ttccgttggc acctggtaag tcgcaccgcc aacccttcgc
    30961 gcccgcactt cgagcacagg catgacattc tttacagccc gctcaaacgt atccaaaggc
    31021 tccttgccgg tcttagcctg gacaatatct gcggcctcgt agagtattga ttcagctacg
    31081 ctcttctttc catccagcat caacttgctt atgaactcag ccatcagctc gctgttataa
    31141 accggatctg gaaccgtttc atgcttgact attgtacctc gccttggcat cactcaccct
    31201 cccctcgcgc tcaacagcat tacttcgggc gcttcgcgcc atacttcgaa cggccctgac
    31261 gtctatctgc aactccagca gcatcaagag tgccgcgcac gatgtgatac ctgacaccag
    31321 gcacatcctt gatacggcca cccctgatca gcacgactga gtgctcctgg aggttatgcc
    31381 cttcacctgg aatgtacgct gtaacctcaa gtccgtttgt cagcctgacc ctggcgatct
    31441 tcctaagggc cgaattcggc ttcttcgggg tagtcgtcct cacaacggta cacacgcccc
    31501 tgcgctgcgg agcgcccttc ccataggttg acctgcggtt caaagagttg tactggtacc
    31561 tgagtgcagg cgatgcagac ttagcctttg actgcttacg gccttttctt accagctggt
    31621 taatggtcgg cacaatgacc cacctccttc ccatagagtc agacgctgcg gacagaatat
    31681 gccccttcca cctgagtcca ggtgcaaggg acactctcta ctcatggagt atagcagcgg
    31741 aagcggcgcc cacatcgatc ccacatgcct tacccagctc ttccatggaa tcgaccatta
    31801 taataggtac gccgtttctc acacataacg ccttcacatc acggataacg tgctcctcag
    31861 catcgcttgc gatatatacc gcactcacgc accctcgctc caaagctctt accgtctgct
    31921 ttgtgccgat cactcgacta tctgcgtcgg caagaccctg gttcatgggt gttatcgctc
    31981 ctttcggcat atcgtttcaa gccctagctt cagaggctca gacagtttta
//
LOCUS       DAOSYL010000023        31561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1166_length_31561_cov_8.470799, whole genome shotgun sequence.
ACCESSION   DAOSYL010000023 DAOSYL010000000
VERSION     DAOSYL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 31561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31561
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_31561_cov_8.470799"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..1959)
                     /gene="gcvPB"
                     /locus_tag="PLU15_05460"
     CDS             complement(490..1959)
                     /gene="gcvPB"
                     /locus_tag="PLU15_05460"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOO30403.1"
                     /translation="MKGPQPLIFELSIPGRTAVSLPEPAVPARELAELFPEEYLRKDP
                     PALPEVSEVDVVRHFVNLSQLNHAVDTGFYPLGSCTMKYNPKVNEDAARRPGFAAIHP
                     DQDESTVQGAMELLYTLEEYLCEICGMDNFTLQPAAGAHGEITGLFIINAYHESRNDK
                     RTKVLVPDSAHGTNPASAASAGMKIVQVKSDDRGNVDIEDLKAKAGPDVAALMLTNPN
                     TLGLFDEHIGEIAEIVHGAGALLYYDGANANAIMGKSRPGDIGFDVIHLNLHKTFSTP
                     HGGGGPGSGPVGVKKFLSPFLPAPVVRFNEATGKFFWDSDRPQSIGRVHSFNGNFGVM
                     VKTWAYIRSMGPDGLRAASEYAVLNANYIMRKLQPYYDLPYDRACMHECVLSGSRQKK
                     SADVKTLDIAKRLLDFGFHAPTVYFPLIVDEALMIEPTETESKETLDSFIDAMIRIAK
                     EAKDDPDVVREAPHDTPVGRLDETTAARKPILRYTAEEE"
     gene            complement(1956..3314)
                     /gene="gcvPA"
                     /locus_tag="PLU15_05465"
     CDS             complement(1956..3314)
                     /gene="gcvPA"
                     /locus_tag="PLU15_05465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOO30404.1"
                     /translation="MGSPHRYIPNTDQNVADMLADIGVSSIDDLFSVIPPEFRLTEPL
                     SLPKALSEPELSAELNRVAAKCAHSMQYTSYLGGGLYEHFVPSVVRHMLTRGEFYTAY
                     TPYQAEVSQGTLQAIFEYQTMICELTEMDVANASLYDAGTAVAEAAVMSCGATRRNKV
                     VVSRAINPRYRQVLRTYAHPRGIEIVTVDYDTQSGATPPESVPKDLLDDAACFIVQSP
                     NYFGIVEDMAALSEATHRSRALFVAVVNPISLGILAPPGSYGADIALGEGQPLGNPIS
                     FGGPLLGFFATTSKLMRKMPGRLVGQTHDDQGRRGYVLTLQAREQHIRRESATSNICS
                     NEALCALAATIYLSLMGPSGLRKVAELCTWNAHYLHDKIAKIPEYEFPFDGPFFNEFA
                     VRGRRPPAERNRRLLERGIIGGKMIEADYPELSGCTLWAATETRTRQELDTLIRELEV
                     LA"
     gene            complement(3314..3697)
                     /gene="gcvH"
                     /locus_tag="PLU15_05470"
     CDS             complement(3314..3697)
                     /gene="gcvH"
                     /locus_tag="PLU15_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOO30405.1"
                     /translation="MSSKMRFTSDHEWVELEGNIATIGITEHAAEELGDVIYVELPAV
                     GDTFAQGDNLAVAESVKAAADIFAPIGGTVTEVNDALEDEPGLVNEDPMGNGWIVRID
                     VDDVTELDDLMDEEEYEEYLKEEEQ"
     gene            complement(3705..4814)
                     /gene="gcvT"
                     /locus_tag="PLU15_05475"
     CDS             complement(3705..4814)
                     /gene="gcvT"
                     /locus_tag="PLU15_05475"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOO30406.1"
                     /translation="MSELRKTPLFEAHESLGGKIIDFGGWALPVQYSGILDEHRAVRE
                     RAGLFDVSHMGEIHIKGPQAQAAIDHLVTNDCSKAKIHQLVYSPMCYPDGGVVDDILV
                     YKLCEDDYWLVVNAANTDKDFDWVKRNVSGYDVTVENISPQVAQIAIQGPAAQRILQK
                     TTPCDLSSIKFFWCLPDVEIAGRKALVSRTGYTGEDGFEIYCRPEDARHIWDTLLAEG
                     KDEGIVPAGLGARDTLRFEVALPLYGHELSPEITPLEAGLGFFVKLKKPDFIGKDALA
                     AQKEKGLTRKIVGLELMGRGVPREGYAVMSDGKQVGYLTSGAFSPTMNKGLALALVAM
                     EFTEIGTELDIDIRGKACPVKVVKTPFYQKSYRKE"
     gene            5082..6455
                     /gene="lpdA"
                     /locus_tag="PLU15_05480"
     CDS             5082..6455
                     /gene="lpdA"
                     /locus_tag="PLU15_05480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOO30407.1"
                     /translation="MYDVVIIGGGPGGYVCAIRAAQNGLSVALVEKDKLGGTCLNRGC
                     IPTKALAHTAEVMDSAQRASELGLVIPEVGVDFPRVMAKKEKVVAKLRGGVNLLIKRR
                     KVDFVSGVGTLAGTRAVEVAAPDGSVSRLEARNIVIATGSEPITPRIFGYDGVNVLTS
                     EDVLKLSALPKSLLIIGAGVIGCEFASIFRTFGCDVTLVDIMPNILPMVDDDVADVVR
                     KSFKKRGIDIHTGTKIASVNIVNGQVEATTEDGSVFRAEKAILSIGRRPSTGGVGAPD
                     VGIDVGPAGEIIVDSKMRTNVPGVWAIGDVSNKAQLAHVASAQGLVVAANIAGEKCNM
                     EYFAVPNCIFTSPEVAAVGVSERGAQEACIDYKVGKFPFVASGKAMAMGETDGFVKVI
                     SDGSGRVIGGQIVGPHASDLIAELALAVSRQLTLEDIASTIHAHPTLAEAVCEAAEAA
                     MGRAIHI"
     gene            6794..7285
                     /locus_tag="PLU15_05485"
     CDS             6794..7285
                     /locus_tag="PLU15_05485"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOO30408.1"
                     /translation="MSNRTLAGIALVILGTLLLMSNFGIRARFPAASFWPLLLALVGA
                     MFIRDALRPGGERSHVFVGVIILGIGIMYFLKAIGLIRIGMGKLWPVYLVIVGIAFFA
                     QAAAMNVPHYSGTGALLCGVGIILLAFNLRLVDYGIFREAVKYWPVLIIAAGVLKLVD
                     SYR"
     assembly_gap    7747..7846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8585..11398
                     /locus_tag="PLU15_05490"
     CDS             8585..11398
                     /locus_tag="PLU15_05490"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO30409.1"
                     /translation="MRKRGIQDEIAGGQYSPGRLWGGVAIGFVCGSATLFGAYAPFGI
                     VCVTAALFGWDVAYAMPTIAGALAGAALGAGPQAAVGLAISAALLEVLRKLIWPQLSP
                     GFRHSTGSWAVGAIVLLCSFIGSAIAYEVSGSGVDVLVVSLVESALAGSAAAIVVPSA
                     IACAETGQLKSAGRLESAALLVVGAAVCAGLDSWSFGGFSPGAAVGLYASAVAGAIAG
                     PGAGASVGASLGVGLGIARAGQGILIGILTLAGAASGIVGRQGRAVTAFSILIAAALS
                     GYQLRTTVEFTARMVEASVAAIGFLLTPDRLLSELSWRLPGGIAPYNLERERMDGVYH
                     VVMGKLSVLGQTFRELAIAYQGGPIGDFRDTDGGERKDGSDADGHDGAGSAADIDLAG
                     GLGPAEIWSSDVQELDDPFEAASCESPGMRAVRAAAFIRTQACTGCPRYDVCWRDMFF
                     RTYRDIVDMLAAVELFGDGGIESLPEGLKNRCMRTEKVIGASLVAAGRRRSAAVWQGQ
                     KERNGQQKTEALLDRSSTVSILALQMAGVADAISRVSCQAQGMARFDEEAERLIRRRL
                     SAMGIGVVDVRVSWTGHGGLEVYVVKAPCAQDGECVRTVAPAVGDALGRPLSVWQADC
                     FWTEGEGSMTLGPEGKSRVGAATGLESVRRDRRGERKECCIRLLPARKYDLESEAVTL
                     PRTPGETSGDSHAVINLGDGRMAAIIADGMGVGEEASAESGAAVAMLARLLAAGLDLD
                     FAVQSVNAMMLLRSEDDTFTTVDLAVVDLYTGQAEMVKAGAPPSFIKRGHEVIAVGGP
                     SVPVGVLAPTRVMRYRTNLEEGDMLIMVTDGVTEARPGPSESGVELVRVMERADQDWP
                     RKVVADVIDWARGADFMDEDSSGQSRGGTRQATAKHGNRAEARRGNPMGPTGDKKRSG
                     SAQSRDDMTVLAVRLTLAKGAHT"
     gene            11645..13441
                     /gene="tilS"
                     /locus_tag="PLU15_05495"
     CDS             11645..13441
                     /gene="tilS"
                     /locus_tag="PLU15_05495"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOO30410.1"
                     /translation="MGGHPLNVTAKKCIVRHSMIDKGDGIVIGVSGGPDSVALLFFLL
                     DMAKEYDLRLHIAHVNHMIRGADADADADYVEKLAESLRVPVTIERVDVPGVARRSGA
                     TVEDAGRSVRYGLFLRIAKDLGFSKVAVGHNADDQAETVLMRVIRGAGTRGLAGIPPT
                     RDLGEGITLIRPLIGTSRDSIEAYCRAKNVCPRQDATNLDTSYFRNRVRRELLPLLEA
                     NYNSNIRASLTRLAATAADDEAYLRSAADQLLELAIARAGAKVVEQVRYVRPRVEHET
                     EYVTESPIQTAPLQLDLGVLQDAPPALLARVLTAAFAEMADGEHDLYYPHVAAMCDLV
                     AEGSVGDSVDLPRGVRAWLTYDCLLVGHAEDGNLGEEAWDRRQQGQKDRHDWASLKVP
                     GVTSAPACGVIIYSAICEAPQDGAGLACAGHVEADGNAHDDPDGRTAIAMFSRSEWAD
                     SEDGAGKDAGGSARKDSEPSGAMIGPSSLAAIGNMACAASGFEGVDLDPSLLGITWVI
                     FDAGKVDTENLAVRPWKAGDRLAPFGMEGTKKVSDVFIDEKIPKADRLQWPVIVYGDE
                     VFWVVGLRASRIAVVDAKTRRYGVLVAEWDRD"
     gene            13832..15802
                     /gene="ftsH"
                     /locus_tag="PLU15_05500"
     CDS             13832..15802
                     /gene="ftsH"
                     /locus_tag="PLU15_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOO30411.1"
                     /translation="MKSLALWLLLGLIAISISNALFSSKPRVDEITASQFMQYWQEHR
                     VEKLQITGENSIKGTLDDGSGFKLYYPDVKGLVEDLRNDPDLANQVELSMVPEGQGAW
                     WIALIPNIISIILLFVVFFFIMNQVQGGGNKAMQFGKSRAQLQTSERGKVTFQDVAGI
                     DEVVDELEEVVDYLKHPKRYIELGARIPRGILLVGPPGTGKTLLAKAVAGEADVPFFS
                     VSGSDFVEMFVGVGASRVRDMFNEAKRNAPCIIFIDEIDAVGRQRGAGLGGGHDEREQ
                     TLNQLLFEMDGFDPNSGVIVLAATNRPDILDSALLRPGRFDRQIVVDVPDQKGREEIL
                     KVHARNKPLDPEVDLKAVAQRTPGFTGADLANLLNESALLSARNNKKKIGTEEISEAI
                     ERLVAGPAKKGRIMGEREKNLVAYHEAGHAILGHMLPDADPVVKVSIIGRGRAGGYTL
                     MIPEDDRRYRYATKNEMFNEIVVALGGRVAEEIVLGEISTGAQNDLERASKTARKMVM
                     EYGMSDLGPITYGRSHEEQIFLGRDIARDRNYSEEVASRIDAEVHRMVTEAYNKATEM
                     LTQNMDKLHLLATALKERETIEGEELDALLSDDNGAQDSDYGADKSDAGLGSVAAVGG
                     SQPEEAKSGIAAIEERLKAGKQRDPGPEGAPA"
     gene            15993..17477
                     /locus_tag="PLU15_05505"
     CDS             15993..17477
                     /locus_tag="PLU15_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOO30412.1"
                     /translation="MSEPDSGRLPRVYWGTAIAAGAFAVARFVPLPDVVAASGEILSL
                     TATGRSALGIVMFTAILWALEVFPFPVTALLGAVMLPLFGVMGWTDVIKSGAGSDIVP
                     FLLGIMILSTGVVESGLALRVSKRIVRMAGSDPKRVVLAFLLFSGFCAAWLGNLAVAA
                     ILQPIAVAIIVSNGLVPGHSNFGTALSIGCAWGALIGSIATPAGGASNLIAIGFLRNL
                     AGVDLGFTQWMAVGFPAAMLMMAPAWAILIMAFPPEIEELRVDSADADDGECGSEGKS
                     RGAIVANGSPLTAHEIWALVGLGVMLTLWFLSDWLKKAFGMDMPMSLGAIIGAVVYLL
                     PVNGTSSWDVLEHKINWGNLVLVAVGMSLGAGVYDSGAAAWLANAVFGRMTALQPFPR
                     ALLLSTAIMLFKLMFSSNTATASIVMPVVLAAINGAAVDPWTFLAPAALASPLAFILV
                     TSAPANLVFHDSECFSVRDMAVVGIPMTIVAAICIAFVCTLVLG"
     gene            17918..19447
                     /gene="hutH"
                     /locus_tag="PLU15_05510"
     CDS             17918..19447
                     /gene="hutH"
                     /locus_tag="PLU15_05510"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOO30413.1"
                     /translation="MVHVAVKGKDLTVREVVAVAREGATVSLSSEARFRIAEASGFVE
                     SLLERDRPVYGVTTGFGKFADVVISPQDAEELQRNLLLSHASGVGETLPREVVRGILL
                     LRANALACGYSGVRVEVVETLLSMLNAGIHPVVPSQGSVGSSGDLAPLAHTMLVMIGE
                     GEAEYEGQVMDGASAMSKARIPIVALRAKEGLALINGTQVMTSIGALAVWDVARLAKV
                     ADIAAAMSAEALRGTTAAYDSRVHAVRPHPGQAASARNLLRLMEGSGIRASHMDCPKV
                     QDAYSLRCAPQVHGAARDAIDYALRAVETEMNSVTDNPIIFADDGDVISGGNFHGEPM
                     AMAMDFLGIAASELASISERRTERMVNPQLSELPPFLTEHGGLNSGMMIAQYTAAALV
                     SENKILASPAVVDSIPTSGNQEDHVSMGTIAARKARQIATNVANVLAIEIVCAAQGID
                     FLAPLEPGAGTRAAHRAVRERVAHLDADRILAVDIARVREMIESGDLLREVEKAVGEL
                     E"
     gene            19521..21170
                     /gene="hutU"
                     /locus_tag="PLU15_05515"
     CDS             19521..21170
                     /gene="hutU"
                     /locus_tag="PLU15_05515"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOO30414.1"
                     /translation="MARVIRAPRGTELSCKGWQQEAAMRMLMNNLDPEVAERPEDLIV
                     YGGAGKAARNWECFDAIVDSLKELEGDETLLVQSGKPVGVFRTHPDAPRVLISNAMLV
                     PHWATWENFWELEAKGLTMYGQMTAGSWIYIGTQGILQGTYETFAAAAKKHFGGSLKG
                     RLILTAGLGGMGGAQPLAATFNDAVVIAVEVDRERIERRLKTDYCEMVTDSIDEAIAL
                     AREAQEKGIPRSIALLGNAAETHPEFVRRGIIPDLVTDQTSAHDPLNGYIPAGLTLEE
                     AGELRKSNPEEYKRRAMESMAIQVRAMLDMKDRGSVVFDYGNNIRQRAFDAGVKDAFD
                     FPGFVPAYVRPLFCEGKGPFRWVALSGDPEDIYVTDEVIMEHFAYDEHLVKWIQMAHD
                     KVAFQGLPSRICWLGYGERARFGLMINDLVAQGKIKAPIVIGRDHLDCGSVASPNRET
                     EGMKDGSDAIADWPVLNALLNAVNGATWVSFHHGGGVGIGYSLHAGMVVVADGTPEAA
                     VRLERVLTGDPGTGVMRHADAGYEKAQAFAAGHGIKIPKIG"
     gene            complement(21266..22105)
                     /locus_tag="PLU15_05520"
     CDS             complement(21266..22105)
                     /locus_tag="PLU15_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOO30415.1"
                     /translation="MSDKIIAEIKCLRHVYPDQTEVDLCGLDFSVRRGQRVAVLGANG
                     SGKSTLIFHLLGLLAPVDGKVSVFGLSPSKQFKEMRNRVGVVLQNVDDQIIGPTVWDD
                     VTFTPRALGWPRDEIEAKGKAILNRLGIWHLRRKVPHYLSGGEKRKVALAGALITEPE
                     LLIMDEPFTGLDPRSRTELASLLAQLHAESEITYIVSTHEIDYVPAIADWVYVLAGQG
                     KMALSGTPEEVFAQTDALRDAGIEPPVLADLFESLKKRGWDVSVPLTVDQALEELDYA
                     RVR"
     gene            complement(22102..22806)
                     /locus_tag="PLU15_05525"
     CDS             complement(22102..22806)
                     /locus_tag="PLU15_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO30416.1"
                     /translation="MDIRFIDELAANGRTPLHAALPWAKVLASALLLTSTIASSSPAM
                     AGLVAGAVVLLACATKQPLWYTARIAIYPVVFAALFAIGSVWSNPLYAITIVTKAFTA
                     GLIAATLITTTSFVDVFSIISHVLPSLVADALFMGYRAFFILVDELQGLMVAVRLRGG
                     VSGTLPQQLRTYGQMLGVLVIHSADMTERMYRVMQVRGYSQRISATSEHRPPNAMDFA
                     LLAYSALTLVGVFLYR"
     gene            complement(22809..23615)
                     /locus_tag="PLU15_05530"
     CDS             complement(22809..23615)
                     /locus_tag="PLU15_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOO30417.1"
                     /translation="MSHLHIPDGVLPLPWILAGFGATAILLLFAVSSIRHQDRARTVP
                     RIAILSAVMLLAMSLPIPVLHYHVNFTVLTGMMAGPAEGFIAGFITNLILALGAHGGI
                     TVIGLNTLITGLEITLGWLLWSLFGSRKGRPFVKTAIITIIVLAVSTTAMLGIVAGTE
                     INPSLVMHHHHTHTVHEEEEPFDEADHLLPDHDSHEHDPDSDTRLASFKTFATIVYTA
                     GIVGWAIEAVITGSVVQFISESRPDLIGMDTLPVPGDDDDEEEPRRTGEA"
     gene            complement(23619..24194)
                     /locus_tag="PLU15_05535"
     CDS             complement(23619..24194)
                     /locus_tag="PLU15_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOO30418.1"
                     /translation="MAPQNGSDEPVKPAPSGAAAAAAVQSPIEFSATPVAQNWYENAI
                     CMLRNSGCKLTEQRRLILLLFEHGPGHLTAQQVFESVTRSQPDISLVTVYRTLELLKD
                     VGVLSKINFNDGADRYERNLGNAGHHHHLICTGCGRVVEFGDCEISSLARKLEQATGF
                     TIDSHWLKLTGQCATCRLAAPASTPGTNKEG"
     gene            24861..25757
                     /gene="ftcD"
                     /locus_tag="PLU15_05540"
     CDS             24861..25757
                     /gene="ftcD"
                     /locus_tag="PLU15_05540"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOO30419.1"
                     /translation="MQQVVECVPNFSEGRRPEVVREIVAAIEAVSGIRVLDYSSDTSH
                     NRTVVTFVGTPEACEEAAFAGIARACDLIDMAKHHGEHPRLGATDVVPFIPISGVTME
                     ECVEIARRVGQRVGEELEIPVYLYEEAATRPERRNLADVRRGEYEGLKESIGTPERAP
                     DFGPARVHPTAGATIIGARMPLVAFNVNLGTSDIGIAKKIARAVRESSGGLANVKALG
                     VMLEDRQIAQVSMNLVNYVKTPIPRVMEMVRREADRYGVSVVESELIGLMPMDAVLDT
                     AAYYLQLPKLEQSQIIEARVWE"
     gene            25853..27247
                     /gene="hutI"
                     /locus_tag="PLU15_05545"
     CDS             25853..27247
                     /gene="hutI"
                     /locus_tag="PLU15_05545"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOO30420.1"
                     /translation="MGKMGAGFRPRTEPSDLLIIHAAELGTCRARAAWDNGRPEPGPR
                     LGADLGCAGIIEDGAIAVRDGVIVDIGATEEVLSREPEWLARRPNGAGQVADFPEAIR
                     SVTEVFDASGRTVVPGFVDAHTHLVYAGSRYREHLMRLEGAEYLDILKAGGGILATLE
                     ATRKASFDELLELALKRLDVMLLHGTTTVEAKSGYGLDLDTELKQLRVARAACDDHPI
                     EIVSTFMGAHAVPPEFTGDPDGYIDFMIAEVLPQVAEEGLAEFCDVFCEEGVFSVEQS
                     RRVLMAGIAHGLCPKMHADEIYPIGGAELAGELGAASADHLAAASKAGMESLAHAGVV
                     AVLLPGTMFTLMSERYANAREMINLGVPVALATDYNPGTSPVMSMQFVMTLACLKMGM
                     TPQEALCSATANAAYAVGRGGDAGVLRVGAAADFIVCDVPAVDALPFSVGVNPVTDVF
                     VGGVRVVSDRVLSY"
     gene            27268..27903
                     /locus_tag="PLU15_05550"
     CDS             27268..27903
                     /locus_tag="PLU15_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOO30421.1"
                     /translation="MPFGEMTVQSFLDELASNSPAPGGGSAAAVAGAMGAGLVSMVAG
                     LTETAEMSEEDAEKVRQAGARAQKLRTELLRCADEDTRAFNEVMAAYRMPRGTDEEKQ
                     TRREAIQQGLKGAVAVPMRTLELAMEGLGLATYMAFEGNKNAVSDAGVSAQLFNAAVR
                     GAIFNVEINLSGIKDADFVSEARAKVASYDLERERLRKESLDAVLGRLGGN"
     gene            28118..29425
                     /gene="folP"
                     /locus_tag="PLU15_05555"
     CDS             28118..29425
                     /gene="folP"
                     /locus_tag="PLU15_05555"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO30422.1"
                     /translation="MNTDMDNVDNEQNCGSDWPVRCGTGCNHDVRVAVLPCKEDVEAE
                     LLKMGASPAGIEIMAEKGVFRTVILRNVPIKAALIIKQEMLAKGGEAALPHSAIGLCA
                     DSCDILLMGTLRQYERVIHTLALQPFGLLGIAREIQCALASYDGRPESICIGGRTFLY
                     GERTYIMGILNVTPDSFSDGGAYTTADSAFEHAMEMLEAGADIIDIGGESTKPGVDPL
                     PLDEELERVLPIVKRLASQTDAVISIDTYKAEVAERCLDLGAHIINDVSALRGEGDMA
                     SVVSRYNAPVVLMHSKGRPTHMQADPTYECVVREVYSFLAERMEYACERGVSPCNIIL
                     DPGIGFGKTPQHNLELMRRLAEFKSLGRPILVGTSRKSTIGKVLHKPVSDRVWGTAAT
                     VATAIMSGADIVRVHDVREMAMVAQITDAICRGWRGEEDGHES"
     gene            29415..29831
                     /gene="folB"
                     /locus_tag="PLU15_05560"
     CDS             29415..29831
                     /gene="folB"
                     /locus_tag="PLU15_05560"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOO30423.1"
                     /translation="MRADRITLKNLVFYAYHGVFAAEKELGQKFEVDVDMRGDFQAAA
                     RVDDIDMAVNYADVYATVKDLVEERDFDLIEALALNIADEVLSGYEVEEVTVRVRKPS
                     APIGGPADYVECEITRSRSDMAPLGEEDDGLDDPAL"
     gene            29815..30609
                     /locus_tag="PLU15_05565"
     CDS             29815..30609
                     /locus_tag="PLU15_05565"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOO30424.1"
                     /translation="MIPHFRIERLDEVDSTNSYARALAEAGAPDGTVVVAERQTAGRG
                     RLGREWHSSGDFGLWFSLLLRPEFEPQFAGMLGIAAGVAVVQSMRSLVGARAVLKWPN
                     DVLIDGRKVCGILSEGRTVGQKLEWALIGVGINVTAPEGGFPPELEDVATSLAQHCAA
                     DIGAPAPDKEALLDSVLSQFGARYAMLSSGNVRAVRDEAEELMRSFLNRRVTIRGQSG
                     TMEVRTLDLSEDGALLVELDGGEVAKITAADVSIGTRACARGELDA"
     gene            30602..31225
                     /locus_tag="PLU15_05570"
     CDS             30602..31225
                     /locus_tag="PLU15_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO30425.1"
                     /translation="MHNAIALAIAALMVGMSCNSPATAGISGQEGIQVVSPVSVSAGG
                     AGTSALDWTALLRAVTLLGGPEGTLVLSAALLTADPPTGEMVIDASVSTALVVEVLKV
                     GFGRARPYVPGENGRFTGPNLSGKYHSMPSGHSANAFAIATVLAHRYPEQAALAYGLA
                     LAVGLSRIQLGVHWPSDVVAGAAIGLVMGNQVVEGRLHLLELDGATD"
BASE COUNT         6642 a         7827 c         9788 g         7204 t
ORIGIN      
        1 tggttcagtt cgtccccgag cattctcaag atatacgcga ggaatctttg tgcctctcga
       61 gctcgcgtga tctgtcaaaa cagatccccg agccagttat cactcacggc atctgccaga
      121 tcattgtgga ttgccgttag ggccttcagt tgtccgtcgc tcaccttcca ttgggtcacg
      181 gtgacgacat ccgctgccac ctgaaacgtg aagcgggagt ctctgtctga cgtaagataa
      241 tcatccacct agctagatga aaatgagctt tcccaaggtg gagagtggat gtcagcctac
      301 agaaggctgc ttgcagtgca ccgaaaaagc ctggtatccg tcgggtatct acaccgattc
      361 ggaaaccagg cttttctggt atgcttcctc atgtccatgc aacgcttcgg gtttcatggc
      421 cgactacagc ctatacttat cacccgcaat tcacaagccc tgcatggtgg gacagtgctg
      481 ttagttttgc tactcttcct ccgccgtata cctgaggatc ggctttcttg ccgcggtcgt
      541 ctcgtcgagc cttccgaccg gtgtatcgtg cggcgcttcg cggacgacgt cgggatcatc
      601 cttcgcttct ttggcgatcc tgatcatcgc gtcgatgaag gagtcaagcg tctccttgct
      661 ctcagtttcg gtgggttcta tcattagcgc ctcatctaca atcaacggga agtagactgt
      721 gggcgcgtgg aacccgaagt ccagcaggcg cttggcgata tccaaggtct tcacgtcagc
      781 cgatttcttc tggcgcgatc ctgacagcac gcactcgtgc atgcacgcgc gatcatacgg
      841 aagatcgtaa tacggttgaa gcttccgcat gatgtagttg gcgttgagca ctgcgtactc
      901 gctcgctgca cgcaggccgt caggccccat cgaccgaatg taggcccatg ttttcaccat
      961 gacgccgaag ttgccgttga atgaatggac tcggccgatc gactgtggtc tgtcgctatc
     1021 ccagaagaac ttgcccgttg cctcattgaa ccttactacc ggtgcgggca ggaatgggct
     1081 gaggaacttc ttgacgccca caggccctga tcccggtccg cctccgccgt gcggcgtgga
     1141 gaaggtcttg tggaggttga ggtggataac gtcgaatcct atgtcgcccg ggcgggattt
     1201 gcccatgatg gcgttggcgt tggcaccgtc gtaatagagc aatgcgcctg cgccgtgtac
     1261 tatctcggcg atctctccga tgtgctcgtc aaagaggcct aaggtatttg ggttggtcaa
     1321 catcaatgct gcaacatcgg ggccggcctt ggccttgaga tcctctatat ccacgttgcc
     1381 cctgtcgtcc gatttgacct ggacgatctt catgccggcg cttgccgcgc tcgcagggtt
     1441 ggtgccgtgg gcggagtcgg gaaccaagac ctttgtccgc ttgtcattac gcgactcgtg
     1501 ataggcgttg atgatgaaga gcccggtgat ctcgccgtgc gcaccggctg ccggctgcag
     1561 ggtgaaattg tccatcccgc atatctcgca caggtattct tcgagggtgt acaagagctc
     1621 catagctccc tgaaccgtcg attcatcctg atcgggatgg attgctgcaa agcctggcct
     1681 tcgcgcggcg tcctcgttca ctttggggtt atacttcatc gtacacgaac cgagtggata
     1741 gaaacctgta tccaccgcgt ggttgagctg cgagagattg acaaagtgtc tcacaacgtc
     1801 aacttcgctc acctccggaa gggccggagg atccttgcga aggtattctt ccgggaagag
     1861 ttcagcaagt tcacgggccg gaactgccgg ctccggaagg ctgaccgcgg tccgcccggg
     1921 tatgctcaat tcgaatatta gcggctgcgg gcctttcatg ctagcacctc cagttcccgg
     1981 attaaggtgt cgagctcttg gcgggttcgg gtttccgttg ctgcccagag cgtgcaccct
     2041 gaaagctcgg gataatctgc ctcgatcatc ttgccgccga tgattcctct ttccagcagt
     2101 ctgcggttac gctctgccgg tggccttctg ccccgaactg cgaattcgtt aaagaaggga
     2161 ccgtcgaacg ggaattcgta ttcagggatt ttggctatct tgtcgtggag gtagtgggcg
     2221 ttccaagtgc acagctccgc gacttttcga agcccggacg ggcccatgag gctcagatat
     2281 atcgtagctg caagggcgca taaggcttca tttgagcaga tgttgctcgt tgcgctttcg
     2341 cgccttatgt gctgttcgcg tgcttgcagt gttaggacat aaccgcgacg tccttgatcg
     2401 tcatgagtct ggcccacaag gcgtcccggc atcttccgca tcagcttcga ggtggttgcg
     2461 aagaatccaa gcagcggtcc gccgaaactg atgggatttc ccagtggctg cccttctcct
     2521 agggctatat cggcgccgta gcttccgggc ggggccagga ttcctaagga tatcgggttt
     2581 acaaccgcca caaacagagc ccgtgaccgg tgcgtagctt cggatagggc ggccatgtcc
     2641 tctactattc caaagtaatt tggagactgg acgatgaagc aggctgcatc gtccagaagg
     2701 tcctttggaa cgctttctgg aggcgtagcg ccgctttggg tgtcgtaatc aacggtcaca
     2761 atctcgatgc cgcgggggtg cgcatatgtg cgcagaacct ggcggtatct ggggtttatg
     2821 gccctgctta ctacgacctt gttgcggcgg gtggctccgc acgacatcac agcagcttct
     2881 gccacggctg ttccggcatc atacagcgat gcgttggcta cgtccatctc ggtcaactcg
     2941 cagatcatcg tctggtactc gaagatcgcc tggagcgtac cctggctcac ttctgcctga
     3001 tacggggtgt aagccgtgta gaactcccca cgggtgagca tgtgccggac tacactgggc
     3061 acgaagtgct catacaggcc gccgccgagg tatgaagtgt attgcatgga atgggcacac
     3121 ttggcggcga ccctgttcag ctctgccgaa agctcgggct cggaaagagc ttttggtagg
     3181 ctcaaaggct ctgttagcct gaattcaggc ggaataaccg aaaacaagtc gtctatgctg
     3241 gaaactccga tatcggccag catatcggcg acattttggt cggtatttgg aatgtacctg
     3301 tgcggcgagc ccatcactgc tcctcctctt ttaggtactc ctcatactct tcttcatcca
     3361 ttaggtcgtc tagctcagtc acatcgtcta cgtcgatgcg cactatccag ccgttgccca
     3421 tcggatcttc attcacgaga ccgggttcat cttcaagcgc gtcatttacc tcggtgactg
     3481 ttccgccgat aggggcgaat atgtctgctg ctgccttgac cgactcagca actgccagat
     3541 tatcgccctg ggcgaatgtg tctccgactg cgggcaattc gacgtatatc acgtctccaa
     3601 gttcctccgc tgcgtgttca gttataccga ttgtagcgat gttgccttca agttctaccc
     3661 actcatggtc gctggtgaat ctcattttgc tgctcatatc ccccttactc cttcctgtaa
     3721 gacttctgat aaaatggcgt ctttaccact tttaccgggc acgctttccc tcttatgtct
     3781 atgtcgagtt cagtgccgat ttcggtgaac tccatagcca ccagagctag tgcaagccct
     3841 ttgttcatag tcggcgaaaa tgcgcctgac gtcaagtacc cgacctgttt gccgtctgac
     3901 ataactgcgt agccttcgcg cggcactccg cgacccatga gctccagacc aacaatcttc
     3961 cgcgtcaacc ctttttcctt ctgagcggcc agggcgtctt ttccgatgaa gtcgggtttc
     4021 ttcagtttga cgaagaagcc taggcccgct tcgagcgggg tgatttcggg tgatagctca
     4081 tggccgtaaa ggggaagagc cacttcgaac ctgagggtgt ctcgtgcgcc gaggccggcg
     4141 gggacaatgc cctcatcctt gccttctgcc aaaagggtgt cccaaatgtg gcgggcatcc
     4201 tcaggccgac agtagatctc gaatccatcc tctcctgtat atcctgtcct tgatacgagg
     4261 gccttgcgtc cggcgatctc cacgtcgggc aggcaccaga agaacttgat ggatgaaaga
     4321 tcgcaaggcg ttgtcttctg tagtattctc tgggcagcgg ggccctggat tgctatctgg
     4381 gctacttgcg gggatatgtt ttccaccgta acatcataac ccgagacgtt tcttttcacc
     4441 caatcgaagt ccttgtcagt atttgcggcg tttacaacga gccaataatc gtcctcgcaa
     4501 agcttgtaga ccaaaatgtc atccactacc ccgccatccg gatagcacat agggctgtag
     4561 acgagctgat ggattttggc tttggaacaa tcattggtga ccaggtggtc gattgcggcc
     4621 tgagcctggg ggccctttat gtgtatctcg cccatgtgcg acacatcgaa aagcccggct
     4681 cgttcgcgca ctgcccggtg ttcatccaag atgccactgt actgaacggg cagcgcccaa
     4741 ccgccgaaat caattatctt tccgcccagc gattcgtggg cctcgaatag tggggtcttg
     4801 cgcagttctg acattactcc acctcctgcc agataagtat tatgcgccaa ttgaaggaat
     4861 gcttgcgccc agctgacggc tatcccaatg gcgctggcat attcatgctt ggtattcatc
     4921 acttgcgggc aaactccttc tagtcgagtc gtcgggctga gtccgttcgg acgcgtactg
     4981 gccctgcggc aggaacagct gctcggaaaa gcgaactata ctcggactgc tgctgtattt
     5041 ttcccaatgg cggcagccat gatttaggga ggattaatgg aatgtacgat gttgtgataa
     5101 ttgggggcgg ccccggagga tatgtctgcg caatcagagc ggcgcagaac ggcctttctg
     5161 ttgctcttgt cgaaaaagac aagctcggag ggacttgcct caaccgaggc tgcattccaa
     5221 ctaaagcctt ggcccatacg gccgaagtaa tggattcggc acagcgcgcg tcagaattgg
     5281 gtctggtcat tcctgaagtg ggcgtcgact ttccgcgagt tatggccaaa aaggaaaagg
     5341 tcgtcgccaa attgcgcggc ggggtcaatc tccttatcaa gaggcgcaag gtggatttcg
     5401 tgagcggcgt ggggacgctg gctggaacgc gagcggttga ggttgcggct cccgacggtt
     5461 cagtatcgcg gcttgaagcg aggaatatcg ttatcgcgac aggatcagaa cctattacgc
     5521 cccgcatttt tgggtatgac ggcgtcaacg tgcttactag tgaggatgtc ctcaagcttt
     5581 cggctcttcc gaagagtctc cttattatag gggcgggagt cataggatgc gaatttgcga
     5641 gcatcttccg gactttcggg tgcgatgtga ctttagtcga tatcatgcca aacatacttc
     5701 ccatggtcga cgatgatgtt gccgatgtgg ttcgcaaatc cttcaagaag cgtggcatcg
     5761 atatacatac cggtacgaag atagcttctg tcaatatcgt gaatgggcag gttgaagcga
     5821 ccactgaaga cggaagcgtt ttccgggctg aaaaggctat tctgtcgatt ggcaggcgtc
     5881 cgtctacagg cggggtgggt gctcccgatg tcgggatcga cgtgggacct gccggagaga
     5941 tcatagtcga ctccaagatg aggacgaacg tgccgggcgt atgggccatt ggggatgtct
     6001 caaacaaagc tcagctggca catgtggcgt ctgctcaggg attggtggtt gctgcgaata
     6061 tcgccgggga aaagtgcaat atggaatact tcgcggtccc caattgcatc tttacgtcgc
     6121 cggaggttgc ggctgtcgga gtgtcggagc ggggggcgca ggaagcatgt attgactata
     6181 aagtaggcaa attcccgttt gtcgcatctg gcaaggcaat ggccatgggc gagactgatg
     6241 gctttgtcaa agtcatctca gatgggtctg gccgagtcat tggcggacag attgtggggc
     6301 cccatgcatc tgacctcatt gccgagctgg cgcttgcagt ctcaagacag ctgacattag
     6361 aggatattgc cagcaccata catgctcatc caacgttggc tgaggcggtc tgcgaggcag
     6421 ccgaggcggc aatgggacgg gcaattcata tctgagcagg ttatgaggga gcattcggcg
     6481 ccatcagtcg tctgatatcg gaacttcggc ccacgtgaat ttgggtgtgg cgcaaagaaa
     6541 agtcaataca tgagtagtcg tgacaggttc ttgcgcgatc ctaagcctgc agttcggatc
     6601 ttggctgtct actgaaggcc gtgattcctt ttgcatcttg cttggcagcc tagatgcttc
     6661 tcgaagcttg ctcagttagt attgttgcgg gcagtcgcgg cacgtggata tagggctagg
     6721 aaaacggggc tgcaatctgc atgtgacggg gcgcacatct tagagcgctt tgtgaaagga
     6781 ggcgggaacg gtcatgagca atagaacact ggcaggaatt gcgttggtca tcctgggaac
     6841 gcttcttctc atgtcgaatt ttgggataag ggcgcgattt ccggcagcca gcttctggcc
     6901 gctcctctta gctttggttg gagcgatgtt catcagggac gctctcagac caggcggcga
     6961 aaggagccat gtctttgtcg gagtcataat actcggcatc gggatcatgt attttctgaa
     7021 agcaataggg ttgatcagga ttggcatggg gaagctctgg ccggtctacc tggtaatagt
     7081 gggaattgcg ttctttgcac aggcggctgc gatgaatgtg ccgcattatt cggggacagg
     7141 tgctcttttg tgcggagtag gcatcattct cctcgctttc aacttacgcc tggtggacta
     7201 cgggatattc cgcgaggcgg tgaaatactg gcctgttctg ataatagccg cgggagttct
     7261 caaacttgtc gacagctaca gatgatgata tgagcggaag tctcacagcg cgtatgttga
     7321 tggcctgaga cggaaacgaa atgaattgcg ccgacgagta tgggcgcaga gtgtcgttat
     7381 agatcctaat ccacgcgggg cggggcttgc atgatctctt gtcagagatc ggcaggcctt
     7441 gttttcatta tccaagtgta gcttttgaca tgacgccggt tcttttgagc gagtatcgat
     7501 aggcttttct gccattatcc agtctgcatt gctcgtagct ggggctcttg cggctcgatt
     7561 gggccaagat gctttttgcg agttgccctc agagcacagg gcttaagtat attgtattgg
     7621 caactgtcgg ctttatgcga aatgcataat ctcgaacgac tgcggtccag taaagggttg
     7681 agcgaacctt gcgatgtcgc aatttacacg tggaatagca taccgcaggc cctccagcat
     7741 gcttcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncatg cttcagccgg
     7861 aatgtgctct tcttttgttg tcatttctat gcagcttcct gtacggatct acatggttcg
     7921 cgcgatcggc gtgctacgat gatctatggg aatgcccgcc atggtggtag acacggtttt
     7981 tctctgcgag ccgatgtcgg gttttccatg gtctgggtag gatcagaagc ggtcagccgc
     8041 aggcattgga aaagaccttc cagagaggct ataatggcgc gctccgatcg cctgtggagc
     8101 cagcggccaa ggacggctga acgggcggga tttgcaacag attgtaatgg cctgccagtt
     8161 gcccattaga ggatcgtcaa tatcagcgtg cccaatcgac gggtctgcat acgaggtggt
     8221 tccagtcgat agacggggcc aggagggttg atatatggtt gaagtccata ttgagccata
     8281 tacgagggtt acgggcgctg ggtctggcgg cagggggcag acgcgagcag ggggaacgtc
     8341 cgggcggcgt gggagggatg cttcagaggc tgggccaact cctaaccgtg gaaagcgcaa
     8401 accccgaggg ccgaatcgac agacttcttc cgctttgccg cagggcgcag atgcttcgaa
     8461 aaggccaggc acgcgccgac aacctgcgaa agagcgagcc cgacagggaa aatcgtttca
     8521 tcaggggttg ccgaaatggg ggtcctcatg gaacagccgg gctgcaaaag gagaattgcg
     8581 tgatgtgcga aaacgtggaa ttcaagatga gatcgcgggt gggcagtatt ctccgggcag
     8641 gctgtggggg ggcgtggcca ttggttttgt gtgcgggtct gctaccctgt ttggtgccta
     8701 tgccccgttt ggcattgttt gtgtgaccgc agcgcttttt ggatgggatg ttgcatatgc
     8761 gatgcccaca attgcgggcg ccctagccgg agcggccctt ggggcgggcc cgcaggccgc
     8821 ggtcggcctc gccatatccg ctgcgttgct agaggttctc cggaagctca tctggcctca
     8881 gctgtcgcct ggtttccgcc attccacagg tagctgggcc gtgggcgcga tcgtcctctt
     8941 gtgttcgttc attggatcgg cgatagccta cgaggtaagc ggatcaggcg tcgacgtgtt
     9001 agtcgtcagc cttgtcgagt ccgccttggc cgggtcggca gcggccatcg tggttccttc
     9061 ggcaatcgcc tgtgccgaga cgggccagct caaatcggcg ggccgacttg agtcggctgc
     9121 gcttcttgtt gtgggggccg cggtctgcgc aggccttgat tcatggtctt ttggcgggtt
     9181 ttcccccggc gcagccgttg gcctctatgc gtcggcggtt gcaggtgcga tagcgggtcc
     9241 gggcgcaggt gcgagcgtgg gagcatcttt gggcgtcgga ctcggcatcg ccagagccgg
     9301 acagggtatc ctcattggca ttttgacatt ggccggggcc gcctcgggca tagttggcag
     9361 gcagggacgg gcagtcacgg cgttttctat attgatcgca gccgcgctct caggttacca
     9421 gcttcgaacg acagtcgagt tcacagccag aatggttgaa gcgtcagttg ctgcgatcgg
     9481 gtttcttctt acccctgatc ggcttctttc agagctttcg tggcgccttc ctggcggtat
     9541 tgccccttat aatcttgaaa gggagcggat ggacggtgtc tatcacgtgg ttatggggaa
     9601 gctttccgtt ttggggcaga cctttcgtga acttgcaata gcatatcaag gcgggcctat
     9661 cggggatttt cgcgacacag atggcggtga aaggaaggat ggaagcgacg ccgatgggca
     9721 tgacggggct ggttctgctg ccgatatcga tcttgcgggc ggtttgggac ctgcagagat
     9781 ctggtcatct gacgttcagg aactggatga tccgttcgaa gccgcgagct gcgagagccc
     9841 cgggatgcgc gcagtacgtg cggcagcctt cattcggaca caggcatgta ccggttgccc
     9901 aagatacgat gtgtgttggc gcgacatgtt cttccgaact taccgggaca ttgtggacat
     9961 gcttgcagct gtggagttat tcggggatgg tggcattgaa tcgcttccgg aagggctcaa
    10021 aaatagatgc atgcggacgg aaaaagtgat tggagcctcc ttggttgctg cgggaagaag
    10081 aaggagcgct gccgtatggc agggacaaaa ggagcgaaat gggcagcaga agacggaagc
    10141 gcttctcgac aggtcatcga ctgtgagcat cttggcattg cagatggccg gtgtggccga
    10201 cgcgatctcg cgcgtatctt gtcaagcgca ggggatggct aggtttgacg aggaagctga
    10261 aaggttgatc cgaaggcggc tgtcggccat gggcattggc gttgtggatg tcagggtatc
    10321 ctggaccggt catgggggtt tggaggtgta tgtggtcaag gctccgtgcg cgcaggacgg
    10381 agagtgcgtc agaacagtgg cgcctgcagt cggcgatgcg ttgggccggc cgctctccgt
    10441 ttggcaggcg gattgctttt ggactgaagg agaaggttcg atgacgcttg ggcctgaagg
    10501 caagagccgt gttggagcgg cgacagggtt ggaaagcgtt cgtagggacc ggcgaggaga
    10561 gcgcaaggag tgctgtataa ggctgttgcc tgcccgaaag tatgatcttg agagcgaagc
    10621 tgtaacattg cccagaactc cgggcgagac aagcggcgat tcccacgcag tgatcaactt
    10681 gggagatgga aggatggcag ccataatagc ggatggcatg ggggttgggg aagaggcttc
    10741 agcagagagc ggcgcagccg tggcaatgct ggcgaggctc ttggcagccg gccttgatct
    10801 ggatttcgcg gtccaaagtg taaatgcgat gatgttgctc aggtcggagg atgacacgtt
    10861 caccactgtt gaccttgccg tggtggacct ttacaccggg caggcagaga tggtcaaagc
    10921 gggcgccccg cctagcttca taaaacgcgg acacgaggtg atagccgtag gaggcccctc
    10981 tgtgccggtt ggggttttgg cgcccacgag ggtcatgagg tacaggacta atcttgagga
    11041 aggggacatg ctcataatgg tcaccgacgg ggtgaccgag gcaaggccag gcccgtccga
    11101 atcaggtgtc gagcttgtcc gtgtgatgga gcgtgcagat caggattggc cgaggaaggt
    11161 tgttgcggac gtgattgact gggcgagagg ggcggatttt atggacgagg attcttccgg
    11221 acaaagccgc ggagggactc ggcaggcaac ggcgaaacac ggcaatcgcg cagaagcacg
    11281 ccgaggcaat cccatggggc ccacgggcga caagaagcgg agcggctccg cccagagcag
    11341 agacgacatg actgtgcttg cagtcaggct tactctggcc aaaggcgctc acacatgatg
    11401 tggggcgtct tctttgtgat atcatatgaa taagggttct attggttccg ttggcgggcg
    11461 cctttgccaa ctgcggaaat ccgatggccc aaaacaccac aaaccggagg gggctatctt
    11521 gctccttcgg cattgttctg tgcagctcag attgtcagtt gctgcaggat tacagttggg
    11581 gattgcctat tatgtaatga cgcttgatac tgaatctgac gtgaaatgtg ggaggcagcg
    11641 ctaaatgggc ggacatccat tgaatgtcac agccaagaag tgcattgtgc gccattcgat
    11701 gatagacaag ggagacggga tcgtgatcgg ggtctcgggt gggcctgatt ccgtggccct
    11761 cctttttttc ctgctcgaca tggcaaaaga gtacgacctg aggctccaca ttgcccacgt
    11821 caatcacatg atccgtgggg cagatgccga tgcagacgcg gattatgttg agaagctcgc
    11881 cgagagccta agagtgccag tgactataga gagagtagat gtgcctgggg ttgcccgcag
    11941 aagcggggca acggttgaag atgcaggcag gtccgtaagg tatggcctgt ttttgaggat
    12001 cgccaaggat ttgggattca gcaaagtggc tgttggacac aatgcggatg atcaggcaga
    12061 gaccgtgctc atgagggtga tccgtggcgc tggaactcgt ggccttgccg ggattccgcc
    12121 aacccgcgat ctgggggaag gaatcacgtt gatcaggccg cttataggca caagtaggga
    12181 ttcaatagag gcctactgca gagcgaagaa tgtctgtcca aggcaggatg cgacgaactt
    12241 ggacacttcc tattttagaa atcgtgtgcg tcgggagctg cttccgttac tcgaggctaa
    12301 ctacaactcg aatatcagag catccctgac caggcttgcc gcgacggctg ccgatgatga
    12361 ggcctatttg aggtctgccg ccgatcagtt gctggagctt gcgattgctc gggcgggggc
    12421 caaggttgtg gagcaggttc ggtacgtgag gcctcgagtc gagcatgaaa cagagtatgt
    12481 gactgaatcc cccatccaaa ccgcgccgct acagctcgac ctcggagtgc tgcaagatgc
    12541 accgcctgcg ctccttgcca gggtgttgac tgccgcgttt gcggagatgg cagatggaga
    12601 acatgatcta tactatccgc acgtggccgc gatgtgtgat ttggtagctg agggatctgt
    12661 aggcgactcc gtagacttgc ccaggggcgt acgagcatgg ttgacatatg attgcctact
    12721 agttgggcat gcggaagacg ggaacctggg cgaagaggct tgggataggc gccaacaagg
    12781 gcagaaggat aggcatgact gggcaagtct gaaggttcct ggtgtaactt ccgcgcctgc
    12841 ttgcggggtg atcatctact cggctatctg cgaagcgcct caggatggag caggtttggc
    12901 gtgtgcaggg catgtggagg ccgacggcaa cgcccatgat gaccctgatg gacgaaccgc
    12961 catcgctatg ttctccagat ccgaatgggc cgattctgag gacggcgcgg ggaaagatgc
    13021 aggcggttct gcccggaagg attccgagcc tagcggcgca atgataggac cttcatccct
    13081 tgcagcaatt ggaaacatgg cttgtgctgc aagtggattt gagggcgtgg atcttgatcc
    13141 atcgcttctc ggaatcacct gggtgatttt cgatgctggc aaagtcgata cggagaacct
    13201 agcggtccgc ccctggaaag caggggatag gctggccccg tttggcatgg aagggacgaa
    13261 aaaagtttct gatgtattta tagacgaaaa gattcccaag gctgacaggc tgcagtggcc
    13321 tgtgatagtc tatggggacg aggttttttg ggttgtgggt ctgcgggcga gcagaattgc
    13381 tgtggtagat gcgaaaacca ggaggtacgg ggtgcttgtg gccgaatggg atcgggattg
    13441 agagtccggc gccaatacag ggctccgcag ccgcctctgt ctggagtgcc gaagggaccg
    13501 catctgagag tgctgagtgc ttcaggacgg acattctgca ttccgtagga acattgaagg
    13561 gctcatggtc gttaaggata aagtacaacg atacacatag gtcatacggg tcttttggtt
    13621 gtctgtggaa tcattttgtc ttgggccttg ggccttgttt gtgtcgcgca ggagggctgt
    13681 gctataatta cttcgcatat tacgaaattt gaaaccgaat atttccccgt ggcgtactgc
    13741 gcgggatatt ctattaaagg agggaaggcg cattcctcgc catctttgaa ggctggggct
    13801 tgttgcgcct aacaaggtga ataacaggct aatgaaaagt ctcgcgctgt ggttgttgct
    13861 tggcctcatt gcgatatcaa tatcgaacgc gctattctca agcaagcccc gcgtcgacga
    13921 gattacagca agccagttca tgcaatactg gcaagaacat cgggttgaga agcttcagat
    13981 aacgggtgaa aattctatca agggtacact cgatgacgga tctggcttca aactctacta
    14041 ccccgacgta aagggactag tagaagactt gcgtaatgat ccggatctcg ccaatcaggt
    14101 ggagctctct atggtgcccg aaggacaagg ggcatggtgg attgccctta ttcccaacat
    14161 aatatcgata attcttctgt ttgttgtctt tttcttcatc atgaatcagg ttcaaggtgg
    14221 aggaaacaag gcgatgcagt tcggcaagag ccgggctcag ttgcagacgt ccgagagggg
    14281 aaaggtgact ttccaggatg tcgccggaat agacgaagtc gtggacgagc ttgaggaggt
    14341 agtggactac ctcaagcacc ctaaacgcta cattgagctg ggcgccagga taccccgggg
    14401 gatactcttg gttggacctc ctggaacagg caagacgctc ttggctaagg cggttgcagg
    14461 agaggcggac gttcctttct tcagtgtgag cggttccgac tttgtggaga tgttcgtggg
    14521 tgtcggcgcc agccgagttc gtgacatgtt taatgaggca aaacgcaacg cgccatgcat
    14581 catcttcata gacgaaatcg atgcggtggg ccgtcagaga ggcgctggtc ttgggggcgg
    14641 ccatgatgaa cgcgagcaga ctctcaatca gttgcttttc gagatggacg ggtttgatcc
    14701 gaattccgga gtgatcgtgt tggccgcgac taacaggccc gacattctcg actctgcgct
    14761 gctgaggccg ggtagatttg acaggcaaat cgtggttgac gtgcctgacc agaaaggccg
    14821 ggaggagatt ctcaaggtgc atgcgcgcaa taagcctctt gatccggaag ttgacctgaa
    14881 ggcagtagcg cagcgcacac ccgggtttac cggggcggac ctggcaaatc tcttgaacga
    14941 gagtgctctt ctttccgcaa ggaacaacaa gaagaaaatc gggaccgaag agatttccga
    15001 ggctattgag agactggtgg cgggtcccgc caaaaagggt cggataatgg gggagaggga
    15061 gaagaacctg gtagcgtacc acgaggcggg ccacgcgatt cttggccaca tgctcccaga
    15121 tgctgacccc gtggtgaaag tgtccatcat aggacgcggc agagcgggcg gctacacact
    15181 tatgattcct gaggatgatc gaaggtaccg ttacgccacc aagaatgaga tgttcaacga
    15241 gatcgttgtt gcattgggtg gcagagtcgc tgaagagatt gtgttgggcg agataagcac
    15301 tggggcccaa aacgatttgg agcgcgctag caagactgcg cgcaagatgg tgatggagta
    15361 cggcatgagc gatctaggcc ccataaccta cgggcgaagt catgaagaac agatattcct
    15421 cggacgagac atcgcccgcg accggaacta cagcgaagaa gtggcgtcaa gaatcgacgc
    15481 ggaagttcat cgcatggtaa ctgaagctta caacaaggct actgagatgt tgacccagaa
    15541 catggataaa ctccatctgc tcgcgactgc gctgaaggag agggagacta tagaaggcga
    15601 agaactcgat gcgctcctga gcgatgacaa tggggctcag gattcggact atggggctga
    15661 caagtccgat gccgggctcg gctcggtggc ggcagtcggt ggttcccagc ctgaggaagc
    15721 caaatccgga atcgctgcaa tcgaagaacg gctcaaggcc ggcaagcagc gcgatccggg
    15781 gcctgagggt gcccccgcgt aaaaagaggc ggctttgtgg tccgcaacat ttaactgccc
    15841 tggcgcggtg cagcgtcagg gcagtattat gcgcgaggca gccattcgga aggtcgcact
    15901 gcgatctacg agtaatgccg tctctggcga gcataaaacc gtggggaaaa ccggatgtga
    15961 aaagagaaca tgcagctgag aggatgtagt agatgtcaga accggatagt ggtagattgc
    16021 ccagggtcta ctggggaacg gctattgcag ccggtgcctt tgcggttgcc agattcgtgc
    16081 cattgccaga cgtggtggcc gcttcgggag agatcctgag tcttactgca acgggaaggt
    16141 cggcgctggg gattgtgatg tttactgcga tcctgtgggc cctcgaggtt ttcccctttc
    16201 cagtgaccgc cctcctgggc gccgtgatgc ttcctttatt cggcgtgatg gggtggacag
    16261 acgtcatcaa gtccggagcc ggctcggata tcgtgccctt tctgctcggc atcatgatac
    16321 tttcaacagg tgtagtcgag tccggacttg ccttgcgcgt gtcgaagaga attgtaagaa
    16381 tggctgggag cgatccgaaa agggtagtcc tggcttttct gctttttagc ggcttctgtg
    16441 cggcgtggct gggaaacctt gcagttgccg cgatacttca gccgatagct gttgccataa
    16501 tagtctcgaa tggcctcgtt cccggacatt ctaatttcgg cacggccctc tccatagggt
    16561 gtgcatgggg tgccctcata gggagcatcg ccacgcctgc aggaggtgct tcaaatctga
    16621 ttgccatcgg gtttctgcga aaccttgccg gcgttgatct tggtttcact caatggatgg
    16681 ctgtggggtt ccctgcagcc atgctcatga tggcgccggc atgggccata ttaatcatgg
    16741 ccttccctcc cgaaatcgag gagctgcgtg tagacagcgc ggatgctgat gacggtgaat
    16801 gcgggagcga gggcaagagc agaggcgcta tcgtggcaaa cggcagcccg ttgacggccc
    16861 atgagatttg ggctctcgtc ggattgggag tcatgctcac gctctggttt ctcagtgatt
    16921 ggctgaagaa ggcattcggc atggacatgc ctatgtcgct gggcgcgatc ataggcgctg
    16981 tcgtatatct tctgcccgtc aatgggacta gttcatggga tgtcctggaa cacaagatca
    17041 actggggcaa tctggttctc gtggctgtgg gcatgtcatt gggagccgga gtatatgatt
    17101 caggggctgc ggcatggctt gcaaacgcag tgtttgggag aatgacagct ttgcagccgt
    17161 ttcccagggc gctcctgctt tccactgcga taatgctctt taaactcatg ttctcgagca
    17221 acactgcaac ggcttcgata gtgatgccgg tagttctcgc agccatcaac ggagcagccg
    17281 ttgatccctg gacgtttctt gccccggcgg cgctggcttc gccgcttgcg ttcatactgg
    17341 tgacgtctgc cccggcgaac ctggtattcc acgactcgga gtgcttcagc gtacgcgaca
    17401 tggctgtagt aggaatccca atgaccattg ttgcggccat ctgcatcgcc ttcgtatgta
    17461 cacttgtgct tggttgaaat tagggcgtag gtgggattgg cccgcttgcc gccgcaaatg
    17521 ggaaaggcga gatgtgtctt catgtaccgg gctctgaaaa tctccgctcc ctacagcatc
    17581 tgggccgaaa caggccctgt catgcatgac attgttttcc ggagataacg gagcgcttgc
    17641 gggctcgcga taccaatgcc caatcgggaa tgcgggtgca ggagtgaagc attaatagtt
    17701 cttcttagcc ggggcgccct ggcaggaaaa cgcgtgctcg acgacaaaat ttatatggta
    17761 tggttattgc ggttcacatc aatcccgccc gatcgcagct tgggcttttt tataagttgt
    17821 aaatccgctg cccaactgtg gataattgcc ctgtggtttg ccgccgttct gaggttgtga
    17881 tgtttcagct gtaaggtatt ggcgaaagga agggaccatt gtgcatgtag ccgttaaagg
    17941 gaaagacctg acagtgcgcg aggttgtggc tgttgcgcgt gagggggcta ccgtatcact
    18001 ctcctctgaa gcccgcttta ggattgctga ggcgagcggt ttcgtagaat cgctcctcga
    18061 gcgtgacagg cctgtatatg gcgtgacgac tggttttgga aagtttgcag atgtggttat
    18121 atccccccaa gacgccgagg aattgcagcg caacctcctt ctttcgcatg caagcggagt
    18181 aggtgagacg ctgccccggg aagttgtcag ggggattttg ctgctcaggg ctaatgcgct
    18241 cgcatgcgga tattcgggag tcagagttga agttgtggag actctgcttt ccatgttgaa
    18301 cgccgggatt catcccgtag tcccgtcaca gggatctgtg gggtccagtg gcgacttggc
    18361 ccctcttgcc cataccatgt tggtcatgat cggcgagggc gaggcagaat acgaagggca
    18421 agttatggat ggggccagtg cgatgtcgaa agcccggata cccattgtgg ctttgagggc
    18481 caaggaaggg cttgctctca tcaacgggac gcaagtgatg acgtccatag gcgctcttgc
    18541 tgtgtgggac gttgctcggc ttgccaaggt tgccgacatt gcggccgcca tgagcgccga
    18601 ggctctccgc ggcactacag cggcgtatga tagcagagtt catgcagttc gcccccaccc
    18661 cgggcaggct gcatcagcca ggaacctgct gcgcctcatg gaaggaagcg gtatcagggc
    18721 atcacacatg gattgcccca aggttcagga cgcatattca ttgagatgcg caccccaggt
    18781 tcacggagcg gctcgcgatg ctatagacta tgcccttcgc gctgtggaaa cagagatgaa
    18841 ctcggtgaca gacaatccca tcatcttcgc cgatgacggc gacgtgatat ccgggggcaa
    18901 tttccatgga gagccgatgg ccatggctat ggacttcttg ggaatagccg catctgagct
    18961 tgcaagcatc tcggagcggc gcactgagcg gatggtcaac ccgcagcttt cggaattgcc
    19021 tccgttcctc actgaacatg gcggactcaa ttctgggatg atgatagccc agtatacggc
    19081 cgcggccctt gtctcggaga ataagatatt ggcgtctccg gctgtggtcg actccattcc
    19141 aacgtcaggc aaccaagagg accatgtgag catggggacg atcgcggcgc gcaaggctag
    19201 gcagattgcg acaaacgtcg ctaatgtact ggccatagag atcgtctgtg cggcccaggg
    19261 cattgatttt ctggcacctt tagagcctgg cgcgggaact agggcagccc atcgtgcggt
    19321 gcgggaaagg gttgcacatc ttgacgccga tagaattctc gcggtcgaca ttgcgcgggt
    19381 gcgggagatg atagagtcgg gcgatctgtt gcgcgaggtg gaaaaggcag tcggcgaact
    19441 ggagtgactg attgggagag gcttgtgcga ggcataaggc acaaggttgc acaaatatgc
    19501 agggctggag ggatagtgta atggcaagag taataagagc cccgcgtggg accgagctgt
    19561 cctgcaaagg gtggcagcag gaagctgcca tgcgaatgct catgaacaac cttgacccgg
    19621 aagtggctga gcgccccgaa gatctgatcg tatacggcgg agctggaaag gccgcacgca
    19681 actgggagtg cttcgatgcc atagtggact cattgaaaga gttagaagga gacgagacgc
    19741 ttcttgtcca gtcgggcaaa ccggtaggcg tattcaggac tcaccccgat gctccgcggg
    19801 tcctgatttc caacgccatg ttggttcctc actgggccac atgggaaaac ttctgggaac
    19861 tggaggccaa gggcctcacc atgtacggac agatgaccgc cggaagctgg atctacatag
    19921 gcacccaggg catacttcag gggacctatg agacatttgc cgccgctgcg aagaagcatt
    19981 ttggtgggag cctcaagggc aggctgattc tcactgccgg gctcggagga atgggcggcg
    20041 cgcaaccgtt ggccgctacc ttcaacgatg cagtcgtgat tgcggtcgaa gtggaccggg
    20101 agaggataga gcgcagactc aagactgact actgcgagat ggtgaccgac agcatagacg
    20161 aggccatcgc ccttgcccgg gaagcccagg agaaagggat tccgcgctcc atcgcccttc
    20221 tgggcaatgc tgcagaaaca caccccgaat tcgtgaggcg cgggatcata cctgatctcg
    20281 taacagacca gacgtctgcc catgatcctc ttaacggata catacctgca ggcctcactc
    20341 ttgaggaggc tggcgagctt cggaagtcga atccagagga gtacaagaga cgggcgatgg
    20401 agtccatggc gatccaggtc agggccatgc tcgacatgaa agatcgcggt tcagtcgttt
    20461 ttgactacgg aaacaacatt cgccagcgcg cgttcgatgc cggcgtaaag gatgcctttg
    20521 atttcccggg cttcgttccc gcctacgtaa ggcccctctt ttgcgaggga aagggcccct
    20581 tcaggtgggt ggcgctttca ggcgatccgg aggacatcta cgtaactgac gaggtgatta
    20641 tggagcattt tgcctacgat gaacacctgg tcaagtggat tcagatggcc catgacaagg
    20701 tggcgttcca gggcttgccc tcgcgaattt gttggctggg ctacggagaa cgcgccagat
    20761 tcgggttgat gatcaacgat ctcgtggccc agggcaagat caaggcacct attgtgatcg
    20821 gccgcgacca tctggattgc ggatcagttg catcgcccaa tcgcgagacc gaagggatga
    20881 aagacgggtc tgatgcgatt gcagactggc ctgtgctcaa tgcactgctt aacgcggtaa
    20941 acggtgccac gtgggtttca tttcaccatg gcggcggtgt cggcataggc tactccctcc
    21001 atgccggaat ggtagtggtg gcggatggaa ccccggaggc cgcagtcagg ctggaacgcg
    21061 tgcttaccgg agacccgggg accggggtca tgcgtcatgc agatgcaggt tatgaaaagg
    21121 cccaggcttt cgccgccggt cacgggatca agattcctaa gatcggttga gcaagcttcg
    21181 cagtatcctg cgcgatcctc cattgcgact tgctcaagtt aggacagcca ggaaatggcg
    21241 cttgccgcac tgcaaggccg tgcggtcaac gcactctggc atagtccagc tcctcgaggg
    21301 cttgatctac agtgagtggg acggaaacgt cccaccctct ttttttcagg gattcgaaga
    21361 gatctgccag gacagggggc tcgatccctg catcccttag cgcgtcggtc tgggcaaaaa
    21421 cctcttctgg cgtgccggaa agggccatct tgccttgccc tgccagcaca tatacccagt
    21481 ccgctattgc tggaacgtag tcgatctcgt gggtggagac gatgtatgtt atttcgcttt
    21541 ccgcatgaag ctgggcaagg agcgaggcca gctctgtgcg ggaacgcgga tcaagcccag
    21601 tgaaaggctc gtccattatg agaagctcag gctcggtgat tagcgctccg gccagggcaa
    21661 ccttcctttt ttcgcctccg ctcaagtagt gtggcacttt cctcctcaga tgccatatcc
    21721 ctagcctgtt gaggatagcc ttgcccttgg cctctatctc atctcgcggc caccccagtg
    21781 cccgcggggt aaacgttaca tcatcccata ctgtaggtcc gatgatttgg tcgtcgacgt
    21841 tttgcagaac tacgcccact cggttgcgca tctccttgaa ctgctttgac gggctgagcc
    21901 cgaagacgct gaccttgccg tcgactggcg caagcaagcc gaggaggtgg aatatgaggg
    21961 ttgacttgcc gctcccgttt gccccgagaa ctgcgactct ctggcccctg cggacggaaa
    22021 agtcgagtcc gcacaggtcc acctctgttt ggtcgggata gacgtgtctt aagcacttta
    22081 tctcggcaat aatcttatcg ctcatctgta aaggaacacc cccactagag taagtgcgga
    22141 atatgcgagc aaggcaaagt ccatcgcatt gggaggccga tgctcgcttg tggcggaaat
    22201 gcgctgtgaa tagcctctaa cctgcattac tcggtacatt cgctcagtca tgtcggctga
    22261 atggatcact agaactccaa gcatttggcc gtatgtacga agctgctgtg gaagggttcc
    22321 gctgaccccg ccccgcagtc ggacggcgac catgagtcct tgcagttcat ctacgaggat
    22381 gaagaaagcc cggtagccca tgaataacgc atctgccacg aggctcggca ggacatggga
    22441 aataatggag aagacatcca caaacgatgt ggtggtgatg agagtcgcgg ctatcaaccc
    22501 cgctgtgaaa gccttagtca ctatggtaat ggcgtacaac ggattcgacc atactgatcc
    22561 tatggcgaaa agtgcggcaa agactaccgg gtaaatagca atgcgcgccg tgtaccagag
    22621 cggctgcttt gtagcgcatg ctagcagaac gaccgcacct gccacgaggc ctgccatggc
    22681 gggcgatgat gacgctattg ttgatgtgag gagtaaggcc gatgcaagaa ccttcgccca
    22741 cggcagcgct gcgtgcaaag gagtgcgacc gttcgctgcc agctcgtcga tgaatcggat
    22801 atccaagtct aggcctcccc tgtgcgtctg ggttcttctt cgtcatcgtc gtccccgggt
    22861 actgggaggg tgtccatgcc gataaggtct ggacgggact ccgagatgaa ctggacgaca
    22921 gaccctgtaa tcactgcttc tatggcccag cctactatcc cggctgtgta gacgattgtg
    22981 gcgaatgtct tgaaagaggc aagacgggta tcgctatctg ggtcatgctc gtgggaatcg
    23041 tgatctggaa ggagatggtc ggcttcatcg aatggctcct cttcttcatg gactgtatgg
    23101 gtgtgatggt ggtgcatcac aagcgacgga ttgatctcgg tgccggcaac tattcctagc
    23161 atggctgtgg tggacacggc caagacgata atggtgatga tggcggtttt cacaaatgga
    23221 cggccttttc gggaaccaaa caggctccat aggagccagc cgagggtgat ctccaggccc
    23281 gttattagag tattgagtcc gattactgtg attccgccat gggcacccaa tgccaaaatc
    23341 aagtttgtga taaatcctgc tatgaatccc tcagcgggcc ctgccatcat gccagtcagc
    23401 accgtgaagt taacatggta gtggagaact ggtatgggaa ggctcatcgc cagcaacatg
    23461 acggcggaga gtatcgcgat ccggggaacg gtgcgtgccc ggtcttggtg ccttatggag
    23521 gacacggcga aaagaagcag gattgctgtt gccccaaacc cggccaatat ccatggcagt
    23581 ggaagaaccc catcgggaat atgtagatgt gacatctttt acccctcctt gtttgtgccg
    23641 ggtgtggaag ctggagccgc cagccggcag gtggcgcatt gtccggtcaa tttgagccaa
    23701 tggctgtcta tggtgaagcc ggtggcttgt tcgagcttgc gcgctaacga cgaaatctca
    23761 cagtccccga actcaaccac tctgccgcat ccggtgcata tgaggtgatg atggtgcccg
    23821 gcgttgccaa ggttccgttc atatcggtcg gcgccatcat tgaagttgat cttgctcagg
    23881 actcccacat ccttaagaag ctcgagggtg cggtagactg tgacaaggct tatatccggt
    23941 tgagaccgcg ttaccgactc gaacacttgt tgtgccgtca gatgtccggg gccgtgctca
    24001 aagagaagaa ggattagcct tcgttgttcg gtcagcttgc atccggagtt tcggagcatg
    24061 cagatggcat tttcatacca gttttgggcc actggggttg cagaaaactc tataggactt
    24121 tgcacagcgg ctgctgccgc agctccggat ggagcaggtt tgacgggttc atctgaccca
    24181 ttctgcggtg ccatctcatc acatccacac aagaatctaa atgaaagtca tttgcaatta
    24241 tctctcacat tatactcttc tccccctgcc aagcgcaata gaagataagc caagtgcaat
    24301 ttctcacctt tgtggtcagt gtgcattgat atgtaaatcc cgcagagctg ttgtattgat
    24361 actgcgggca gagggtgcag acggcgcgtc ggatgctatt gagaattgct tattgcttca
    24421 ggaagtatgt ctcgtggggg aggtcgtttt atctttgagc cctaagcagc ttattgacac
    24481 gcgttgaact gcctttttcg ggtgagagcc ggtttatgca ttgagcatgt ccaggaaaac
    24541 atccgttggt tcgcctcaat gggaggcgac taatgtcagc tgcgtcttcc ggcggagttt
    24601 gcatgattgt tgagtcgcga ttgctatccg gcgcaagggc ttcagctgtt ccggggagcg
    24661 ctcgaatcgc cgtttctcgg agtgcgtggg ctagaaggaa ttgccgggct cacggagaaa
    24721 gcgtatgcgg taacatacag gtctttgttc cccagaaggt agaatgcaac gcctcgtgag
    24781 gggtcgctcc tcgcgtaggg gtgtggattg gaattgtagg tttttcatgc aggaggtgcc
    24841 ataagttgag tgatagaact gtgcagcaag tagtggagtg tgtcccgaat ttctcggaag
    24901 gcaggcggcc tgaggttgtt cgggagatcg ttgcggccat agaggcggtt tcagggatcc
    24961 gtgtgctgga ctattcgtca gataccagcc acaacaggac ggttgttaca ttcgttggaa
    25021 cgcctgaagc ctgcgaagag gcggcttttg caggcattgc ccgcgcgtgc gacctgatcg
    25081 acatggcaaa acatcacgga gaacaccccc ggctcggggc gaccgatgtg gtgccgttca
    25141 tacctatttc aggtgtgacc atggaagagt gtgtcgagat cgcgcgccgc gtcggacagc
    25201 gtgttggcga ggaacttgag ataccggtct atctgtatga agaagcggcc acgcggcccg
    25261 aaaggcgcaa tttggctgac gtgaggcgcg gggaatatga ggggctcaag gagagcatag
    25321 ggactcctga acgtgcgccg gatttcggcc ctgcgagggt gcaccccact gcaggtgcca
    25381 ctataatcgg cgctaggatg ccgctcgtag ctttcaacgt gaacctgggc accagcgaca
    25441 tagggatcgc gaagaagatt gcccgcgccg tccgcgaatc cagcggcggt ctcgccaatg
    25501 ttaaggcgct cggtgtcatg ctcgaggaca ggcagatagc gcaagtctca atgaacttgg
    25561 tgaactacgt gaaaactccc atcccccgcg tcatggaaat ggtgagacgc gaggcggata
    25621 gatacggggt ttcggtggtc gagagcgagc tcataggctt aatgcccatg gatgctgttc
    25681 tggatactgc cgcctactat ctgcagctgc ctaaattgga gcagagccag ataatcgagg
    25741 cgcgggtttg ggagtagggg cggcaggctt cgactgcgcg gccgcaagat acggcagaga
    25801 ctgagtgtgc ttggcgaaca aggcattgct gaggcaagga gggctcttta tcatgggcaa
    25861 gatgggggcg ggctttcgcc ctaggactga accatcggac ttgttgatca tacacgcagc
    25921 tgaactcgga acatgtaggg cacgggctgc ttgggacaat ggccggccgg agcccgggcc
    25981 tagattaggc gcagatcttg gctgcgcagg aattatagag gatggagcga ttgctgtaag
    26041 agacggcgtg attgtggaca tcggtgccac tgaagaggtg ctgtcgcgtg aaccggaatg
    26101 gctagcccga agaccaaatg gagccgggca ggtggcggac tttccggagg ctatccgttc
    26161 agtgaccgag gttttcgatg caagcggcag gacggtggtt cctggatttg tggatgcgca
    26221 tacccatctt gtttatgccg ggagtcgcta cagggaacac ctgatgcgtc ttgaaggggc
    26281 cgagtacttg gacatcctta aggctggcgg cgggatcctt gcgactttgg aggcgactcg
    26341 gaaagcgtcc tttgacgaac tgcttgagct tgctttgaag cggcttgatg taatgctgct
    26401 ccacgggact acgacggtcg aggcaaaaag cggttacggc ctcgatctcg acacagaact
    26461 gaagcaactg agggttgccc gtgccgcttg cgacgatcat ccgatagaga tagtcagcac
    26521 attcatgggg gctcacgccg ttccccctga gttcacaggc gacccagacg gttacatcga
    26581 tttcatgatc gccgaggtcc ttccgcaagt tgccgaggaa gggttggcag agttctgtga
    26641 tgtcttctgc gaggaagggg ttttctcagt agaacagtcg aggcgggtgc taatggcagg
    26701 cattgcccat ggactctgtc ccaaaatgca tgctgacgag atatacccca taggcggagc
    26761 agagcttgcg ggagagcttg gggcggcatc agccgaccac cttgccgctg cgtccaaagc
    26821 tggcatggaa agcctggctc atgcaggggt ggtggcggtg ttgttgccgg gcacaatgtt
    26881 cacgttgatg tccgagagat acgctaacgc cagggaaatg ataaacctgg gagttcctgt
    26941 tgcgctcgcc actgactaca atcccgggac atcgcctgta atgtcaatgc agtttgtgat
    27001 gacccttgcc tgtctcaaga tgggcatgac tccccaggaa gcgttatgct ccgccacggc
    27061 caatgcagcc tacgcagtgg gaaggggagg agacgcaggc gtcctccggg taggggcagc
    27121 cgccgacttc atagtctgtg atgttcctgc cgtagatgcc ttgccgtttt cggtgggagt
    27181 caaccccgtt actgatgtgt tcgtaggggg cgtgcgtgtc gtatccgacc gtgtgctttc
    27241 ctattgactt ttctaggagg atgctttatg ccatttggtg agatgactgt ccaaagcttt
    27301 cttgatgaac tcgcttcgaa tagtcctgct cccggcggag gaagcgctgc cgcggtggct
    27361 ggggctatgg gcgccggctt ggtgtcgatg gttgcaggcc ttaccgaaac cgccgaaatg
    27421 tcggaggagg atgctgaaaa ggtcagacag gctggtgcca gagctcaaaa gcttcggaca
    27481 gagcttttga ggtgcgctga tgaagatact agagcgttca acgaggtaat ggcggcgtac
    27541 aggatgccgc gggggactga cgaggagaag caaactaggc gtgaagcgat tcagcaggga
    27601 ctcaagggcg ccgtcgctgt cccgatgaga actttggaac tggcaatgga gggtttgggg
    27661 ctggcaactt acatggcgtt tgagggaaac aagaacgctg tgagcgatgc aggagtctcg
    27721 gcccagcttt tcaatgcagc tgtccgtggc gccattttca atgtagagat caacctatcg
    27781 ggcatcaagg atgcggattt tgtctctgag gcccgagcca aagtcgcctc gtacgacctc
    27841 gaacgggaaa gattgaggaa agagtcttta gacgctgttt taggcagact tggggggaac
    27901 tgaaaaccac tcaaggacgt cgatgtagag aggggctgtg gctaggcgag gatcggcctt
    27961 gcctgaatgg ataagtttta caggctaaag ccgaagcgtg gggcgggagc aagttggcaa
    28021 ggcatatgga tcagcctgtg attagtttgg catggtggcg cttgccttgt agcttggatc
    28081 gggcgagcac atttatgact atggaggact gcagctgatg aacacagaca tggacaacgt
    28141 agataacgag cagaattgtg gatcggattg gcctgtccgg tgcgggaccg ggtgcaatca
    28201 tgatgtgcgg gtggctgtgc tgccctgtaa ggaagatgtg gaagcggagc ttcttaagat
    28261 gggtgcaagc ccggccggca tcgaaatcat ggccgaaaaa ggcgtttttc ggacggtgat
    28321 attgcgtaac gtgccgatca aggctgctct gataatcaag caagaaatgt tggctaaggg
    28381 cggcgaagct gcactcccgc attccgccat aggtttgtgt gccgactcat gcgatattct
    28441 tttgatgggc acattgaggc agtatgagcg cgttattcat accctagctc tgcagccgtt
    28501 tgggctttta ggcattgcgc gggagatcca gtgcgcgttg gcgagctatg atgggaggcc
    28561 agaatctatc tgcatcggag gaaggacatt cctttacgga gaaagaacat acataatggg
    28621 catccttaac gtcactcccg actctttctc tgatggcgga gcgtacacta cagccgattc
    28681 cgcattcgaa catgctatgg agatgcttga ggccggcgcc gacataatcg acatcggcgg
    28741 agagtcgacc aagccgggcg tagatcccct gcccctggac gaggaacttg aaagggtgct
    28801 tcctatcgta aagaggcttg catcccaaac agatgcagtt atttccattg atacatacaa
    28861 ggccgaagtg gcagagcgct gcctagacct cggcgcgcac atcatcaacg acgtgtccgc
    28921 tctccgagga gagggtgaca tggcgtcggt agtctcgcgt tacaatgccc ctgtagtgct
    28981 gatgcactca aaggggcgtc ccacacatat gcaggcagac cccacgtatg agtgcgtggt
    29041 ccgcgaagta tacagtttcc ttgccgagcg gatggagtac gcgtgtgaac gtggcgtttc
    29101 gccctgtaac ataattctag atcccggaat aggctttggt aagaccccgc agcacaatct
    29161 cgagttaatg cgacgtctgg cggaatttaa gagtctgggc agacccattt tggtgggcac
    29221 atccagaaag tccaccatag gaaaggtgtt gcacaagcct gtgtcagata gggtttgggg
    29281 cacggctgcc acagtcgcca cggccattat gtccggagcg gatattgtga gagtccatga
    29341 tgtgagggag atggctatgg ttgcgcagat tactgacgca atatgcaggg gatggcgcgg
    29401 agaggaggac ggccatgaga gctgatagga ttaccctgaa gaacctggtg ttctatgctt
    29461 accatggtgt tttcgcggca gagaaggagc tgggacagaa attcgaggta gacgtggata
    29521 tgagagggga tttccaggca gctgctcgtg ttgacgacat tgacatggct gtgaattatg
    29581 cggacgtgta cgctacagtg aaagacctcg tggaggaacg ggatttcgac ctcatcgagg
    29641 cgcttgccct gaacatcgca gatgaggtgt tgtcaggcta tgaggttgaa gaggtaacag
    29701 taagagtacg caagccgagt gccccgatag gtggcccagc cgattatgtg gagtgtgaaa
    29761 tcaccagatc taggtcagac atggcgccgc tcggagagga agatgatggc ctggatgatc
    29821 ccgcacttta ggatcgagcg gctcgatgag gttgattcca caaactcgta tgcaagggca
    29881 ctggctgaag ccggagcgcc tgacggaaca gtcgtggttg ccgaaaggca gacggccggc
    29941 aggggaaggc tgggaagaga gtggcattca tcgggcgact tcggcctgtg gttttctttg
    30001 cttctgaggc ccgaatttga accccaattt gcgggaatgc ttggaatcgc cgcaggagtg
    30061 gctgttgtgc agagcatgcg ctcgctggta ggcgctagag ccgtcttgaa atggccgaat
    30121 gacgtcctca tagacggccg caaagtatgc ggaatactct ccgaaggtcg cacggttggg
    30181 cagaagctcg agtgggcatt gattggagtt gggatcaatg ttactgctcc ggagggcggg
    30241 tttccacccg agctagagga tgttgctact tcattggccc agcattgcgc cgctgatatc
    30301 ggtgctccag caccggacaa ggaagcgctt ctcgactctg tcctcagcca gttcggggcg
    30361 aggtatgcca tgttaagcag cggaaacgtt cgtgcagtcc gcgatgaagc ggaagagcta
    30421 atgagatctt ttttgaaccg cagggtcacc ataaggggtc agtccggcac catggaagtc
    30481 agaacgttgg atctttctga ggatggcgcg ctcctggttg aactggacgg gggagaagtt
    30541 gcgaagataa cggcggcgga tgtgtcgatc ggaacccgcg cctgtgcccg aggtgaactc
    30601 gatgcataat gcgattgcct tggctattgc agccctcatg gtaggcatgt cctgcaattc
    30661 acctgcgacg gcaggcattt ccggccaaga gggtatccag gtggtgtccc ccgtgtctgt
    30721 ttcggcaggc ggagcgggca catctgcgct ggactggact gcgctcttga gggccgtcac
    30781 tttgctgggc gggccagagg ggacgttggt cctcagcgct gctcttctca ccgctgatcc
    30841 gcctactggc gagatggtga tcgacgcttc ggtatccacc gccttggttg tggaggtgct
    30901 gaaagtaggg tttgggcgcg caaggccata tgttccggga gagaacggac gtttcaccgg
    30961 gcccaacttg tcaggaaaat accattcaat gccttctggc catagcgcca acgcatttgc
    31021 aattgcgacg gtactcgccc atagataccc ggagcaagcg gcgcttgcgt atggccttgc
    31081 attggcagtg ggactcagca gaattcagct cggggtccat tggccatcag acgtggtggc
    31141 aggagcggcg atagggctag tcatgggaaa ccaggttgtt gagggacgtt tgcacctgct
    31201 cgagctggac ggagcaacgg attgacgggc atacgaatgg ggcaggtgtg caccgtcgca
    31261 tcctgtagga tgacctcgag cgatgggacg agatagggac agccgggccg cttcgcaaag
    31321 cgcccggctg tcttcgtcat ttcctgcctg tttttagttg ccctgcccct ctgcattgag
    31381 ccggcttcat ccctgctctt tcgtttggac gtcgctcttg cattgatttt cggacactgg
    31441 ctatcgcttt tgccatcatc ccgagcccgt tactctcgca gaattattca tctggtgctc
    31501 ccaatcggtt agtggtgata gcagatctac tcgtccgcgt tctctggcat acaaccgaga
    31561 c
//
LOCUS       DAOSYL010000024        31319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1179_length_31319_cov_7.482536, whole genome shotgun sequence.
ACCESSION   DAOSYL010000024 DAOSYL010000000
VERSION     DAOSYL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 31319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31319
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1179_length_31319_cov_7.482536"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1109)
                     /locus_tag="PLU15_05575"
     CDS             complement(15..1109)
                     /locus_tag="PLU15_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOO30426.1"
                     /translation="MEREVQNKLEKVRRLMLERGLDAMLLVERSNFAWITGGEAPSVD
                     ETTGRVAACAVVTEDSAYVAADEAEIARLNQNSCLEQKGFKLISRSWYEGLPDPDSFG
                     ARNAVGADVLMSGAIYYGDEVAPLRFKLEPEEIDRYRWLGGQLGKALEEVARCVEPGM
                     TEHKISSLLAVRLVDSGIAPRVTLIATDGRNTSNSQPTPTYSRMEKYAMLTVAGSRWG
                     LSASATRMVCIGPIPDHIRAWHDAAIRIEAAAICATRPGQPVSKCFDAAVRAYEDVGY
                     PDEWKKHHLGGATGYLVRDYLATGSSQETVLENQAFAWNPIVHGIRSEDTIIATKDGP
                     DIITQTGNWPMRSMQGNDGSEIARPDILVR"
     gene            complement(1125..1343)
                     /locus_tag="PLU15_05580"
     CDS             complement(1125..1343)
                     /locus_tag="PLU15_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO30427.1"
                     /translation="MPIFEYKCAKCGEKFEELVTGCCADVKCPKCGSDEVKKLFSSFG
                     FKSGSTFSSSSGGSCGSCAGGSCGTCSH"
     gene            complement(1359..2144)
                     /locus_tag="PLU15_05585"
     CDS             complement(1359..2144)
                     /locus_tag="PLU15_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOO30428.1"
                     /translation="MIEVGTCGFSYRDWRGVFYPESISDREMLEWYATQFSVVELDFT
                     YYRMPDAKTIGGMSRRTPENFSFCVKAHRSMTHELSRDSNDIAEAFQTFSKAMAPLVS
                     EGKLGCVLCQFPWGFKRTPENSSYVQRLPALLPDIPIVVEFRNVEWVSQSTFDLLKGA
                     GMGFCCVDEPNLRGLFPPLAVYTSPIAYVRFHGRNAKTWWKHNEAWERYNYLYTEAEL
                     LEWAPKIQRLAEATKRAFVLFNNCHAGQAAVNAKTMLSLLGMA"
     gene            complement(2141..2506)
                     /locus_tag="PLU15_05590"
     CDS             complement(2141..2506)
                     /locus_tag="PLU15_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO30429.1"
                     /translation="MVVPVTDGELLLVRNVRRAGWEFPGGQIEQGESPEEAARRELLE
                     EAGALVDDLKPLCWYTVTNGVNSSKGIVYIAYVLAVGEPSDVQEIAEVKSFGKPPAEL
                     SFKDGFIELVFKLMEGSSP"
     gene            complement(2616..3770)
                     /locus_tag="PLU15_05595"
     CDS             complement(2616..3770)
                     /locus_tag="PLU15_05595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOO30430.1"
                     /translation="MWTRTIAHVDMDCFFAAVEALDNPDYRGKPVIVGGPRDSVRAVV
                     STCSYEARAYGVRSAMPMSQAARLCPHAIFVPGNMARYVQVSREIMRVLEAFTPIMQQ
                     VSIDEAFLDMTGCEHFYTDALDLGTQIKQAIKDASGLTASVGIAQNKFLAKLASSKSK
                     PDGLLVICPEDVDAFLSPLPVRDVWGVGEKGERSLARYGIETVEDLRLWSKEWLVEQY
                     GKWGAQLYDLARGIDDDPVVADEEQKSFGSESTFDEDIADRYELVQYLAQHARRVGSR
                     LRGAGKLARTVTIKVKFADFSQVTRSKTMAEPFDDYDTIYNTACTLLDEIDMGMAVRL
                     IGLSVSGLSDCRQLSLFGSDNRNCAIDDVLDELSQRYGSKGVIRGRELED"
     gene            complement(3777..4361)
                     /locus_tag="PLU15_05600"
     CDS             complement(3777..4361)
                     /locus_tag="PLU15_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOO30431.1"
                     /translation="MSLYEVPVGVSNRHLHLSRADLDTLFGEGYELTVRKDLSQRGQY
                     ASEETVDVVGPKGTISRVRVLGPVRKRTQVEISRTDSFKLGVQPPVRDSGDLDGSPGI
                     KLIGPKAEITIPVGVIVAKRHIHMTPEEAERIGLKDKDIVMTKVNGARALIFDEVLVR
                     VDDSYTWEFHIDTDEANAACLGNGDLVTIIKKVE"
     gene            complement(4451..5797)
                     /locus_tag="PLU15_05605"
     CDS             complement(4451..5797)
                     /locus_tag="PLU15_05605"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOO30432.1"
                     /translation="MTLHTFPDGFLWGAASSSCQIEGATLEDGKGESIWDRFCRIPGK
                     IVNGDTPAIACDHYHRFSEDVSLMESLKLNSYRFSIAWPRIFPVGGGRPNVKGVDFYN
                     RLVDELLAAGIRPMATLYHWDLPQALQDKGGWIARDTAKYFVDYAAYMFEALGDRVDL
                     WITHNEPWVAATLGYGTGEHAPGVSHMPSALAAAHNILLSHGEAVRAFRDLSRQGARI
                     GITLNMSPVYPASSNECDVLAARRWDGCLNRWYADPLFLGSYPEDIMELYLRAFGAPS
                     ISIEDMGIISTPIDFLGVNYYSRAVVKADPEAQPLELSHVRTNRPVTAMGWEVYPEGL
                     YDLLLRIEQDYDPPAVFITENGAAYDDQVSSGGLVDDRERMEYIRAHLIQLHRAMQDG
                     VEVEGYYLWSILDNFEWAFGYSKRFGIIYVDYTTLKRIPKSSARWYRSVIERGSVL"
     gene            6001..6076
                     /locus_tag="PLU15_05610"
     tRNA            6001..6076
                     /locus_tag="PLU15_05610"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6034..6036,aa:Trp,seq:cca)
     gene            complement(6145..6807)
                     /locus_tag="PLU15_05615"
     CDS             complement(6145..6807)
                     /locus_tag="PLU15_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOO30433.1"
                     /translation="MAAIEPRELIVRRVAQELKSGQVVNVGIGLPTFVVNHLAHDVNI
                     ILHSENGFVGMGDPPEPGREDPDLVNAGGQPSSIKPGGAFFDSATSFAIVRGGHVDVT
                     ILGGLQVDQDGNLANWAVPGRIIGMGGAMDLVVGAKRVIVAMEHRTKDGRSKVVRKCT
                     LPLTATAEVDLLVTELCVIEFTPEGAVLREIAPDVTIGEVLAATEARLYIPDEVGVMT
                     VV"
     gene            complement(6809..7459)
                     /locus_tag="PLU15_05620"
     CDS             complement(6809..7459)
                     /locus_tag="PLU15_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOO30434.1"
                     /translation="MPKLRTLDQALEGLTDGMTLMIGGFLAVGSPETLVDEVIRRGVR
                     DLTLIVNDTAMPDRGYGRLFANGQVSTLICCYMGMNPTAIAQKDEGKLAVELCPQGSL
                     AERIRAGGAGLGGILTPTGVGTIAEEGKIKLELEGHTYLLELPLRADIALLRAHVADR
                     AGNLVYRKSARNFNPLMAMAADYVVAEASEIRDIGGIDPDHVMTPGIFVDAVVGVS"
     gene            complement(7478..8293)
                     /locus_tag="PLU15_05625"
     CDS             complement(7478..8293)
                     /locus_tag="PLU15_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOO30435.1"
                     /translation="MDKLIITAALVGAELSREQAPYLPLSPAEIGKAAEEARNAGASI
                     VHLHVRDEEGKPTQDIEVFRKTIEEIKKRTDVIVQISTGGAVGTPIEERMRPLDLAPE
                     MATLTTGTVNFGDEVFMNEPQAIEALAVRMKEKGVKPELECFDVGHIANALRLAKRSI
                     VEEPLYFDFVMGVPGGIPGTVPDLMHCVNSLPPGTMWQVAGIGRAELQLAAVAIVLGG
                     NVRVGFEDNIYYSRGVLAKSNAQLVARIARIAGELGRDVATPDEAREMLELKK"
     gene            complement(8298..8738)
                     /locus_tag="PLU15_05630"
     CDS             complement(8298..8738)
                     /locus_tag="PLU15_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOO30436.1"
                     /translation="MEEVMIRVRMSQSDAHYGGDLVDGAKLMQLFGDVATELLIRHDG
                     DEGLFVAYDMVEFKAPVYAGDYIEARGRIVRTGNTSRAMTFEAHKVIEACRDPKDPSA
                     AFILEEPLLVARASGTCVVPKDKQRKQGNCCGCGQDAETTKKEG"
     gene            complement(8764..9540)
                     /locus_tag="PLU15_05635"
     CDS             complement(8764..9540)
                     /locus_tag="PLU15_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOO30437.1"
                     /translation="MPKVDLERVRPYGDTLDDGKVQLAFSLPLRYGPEAKEAARQLAM
                     KMGFEDVDVVSAHDLGGPFTFFVVYGKCVHSVDVTQLHIPKVEVEQMDYFQINDFIRD
                     KLGRKITVVAACTGSDAHTVGIDAIINMKGYAGEYGLERYPWFRAYNLGAQVPNETLV
                     ARAIETGADAILVSQIVTQKNVHIANLTRLVDLVEAEGIRDDVLLICGGPRISHELAL
                     ELGFDAGFGPGTLPSHVAAYIAQELVRKEHRTSARLTEER"
     gene            complement(9528..11117)
                     /locus_tag="PLU15_05640"
     CDS             complement(9528..11117)
                     /locus_tag="PLU15_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOO30438.1"
                     /translation="MWLLKLNLDTDKIARCRECASDIADEVMKGVERVTTTAIERTVL
                     RLVGVDGVDSAGVPLPNVLVDMLREAGRLGDGAACHMGRAVAATGLSPQAIADKAAAG
                     ELDFASFDDVEDDRARAVLEDAIEASLARIEANAAKRCELIEKLDPGTEPLIYVIVAT
                     GNIYEDVGQAQSAARSGADIIAVIRSTAQSLLDYVPYGATTEGFGGTFATQENFRIMR
                     KALDEVSLETGRYIRLVNYCSGLCMPEIAAMGAMERLDMMLNDSMYGILFRDINMKRT
                     FADQHFSRMLNAYAGITINTGEDNYLTTSDAYEEAHIVLASQFINEALAYRSGLPDEQ
                     IGLGHAFEIDPATTNGFLYEVAQAQMARDIFPNKPLKYMPPTKYMTGDIFKGYLMNGM
                     FNLAGVMTGQSIQLLGMLTEAVHTPHIHDRYLAIRNAKYVFNNARNLGEEITFKRGGI
                     VESRAQQVLNEATTTLEQVRKTGLMAAIEDGVFAGISRTPDGGRGLDGVVERNPEYWN
                     PFAEEIPARLTRGGRCSSCRK"
     gene            complement(11086..12258)
                     /locus_tag="PLU15_05645"
     CDS             complement(11086..12258)
                     /locus_tag="PLU15_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30439.1"
                     /translation="MNQLDSSVARDPGEILLDLALGAPGKCVGIVGLAKNCGKTTVLN
                     RMIRQATSNGITVGVTSGGRDGELYDAITGLPKPPIYLPAGSLVALAESTIHTGSCSL
                     DVVERLGAWTPAGEIVLARVLSPGHVEVIGCNHSNDLMLAVRRMREFGAELILVDGAA
                     GRTFLASPDIVGAFVLATGAALNGSANRIAELTERAVHMFQLPRPPADVVQVAEPIMQ
                     EGVSAILRADGEIEVFPWKTILGREEEIASAIAEDDVALICGRAVGDVLLAEVASRLN
                     QRRRAAPPDTASGPKEPLSPKKQAFMVVAKDPSRLAVGNQGAEAFEASGGQLCVLEKA
                     PVIAISINPTSPWRAPLDPADLLLEISRHIRELPVIDVVAGLSAVGGVAFETQPGY"
     gene            complement(12206..13474)
                     /gene="ablA"
                     /locus_tag="PLU15_05650"
     CDS             complement(12206..13474)
                     /gene="ablA"
                     /locus_tag="PLU15_05650"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOO30440.1"
                     /translation="MQDNDGDDVSMWEGVSKEQWNDWHWQFGNRITTVDQLKSVVNLT
                     PEEEEGVQRCLARLRMAITPHYASLIDPDDPDCPVRKQAIPVVQELSMGEHDLMDPLD
                     EDVDSPVPHVTHRYPDRVLFLVTSVCSTYCRHCTRRRFAGHTDSAITHDELIQGIQYI
                     RETPEIRDVLISGGDPLTLPDDTLEYIISELRAIPHVEIVRIGTRTPVVMPQRITPEL
                     CNMLKKYHPLWLNTHFNHPKEITPESRRACEMLADAGIPLGNQTVLLKGVNDCPHIMK
                     KLVHELVKIRVRPYYIYQCDLSMGIEHFRTSVGKGIEIVERLRGHTSGFAVPTFVIDA
                     PGGGGKIPIAPQYVISQTDTKVILRNYEGVITAYPEPEDKISQCHQCGICEEIPKPAT
                     EGVAAVMDGMVSSLEPVGLERRKRSRRDPS"
     gene            complement(13464..14504)
                     /locus_tag="PLU15_05655"
     CDS             complement(13464..14504)
                     /locus_tag="PLU15_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOO30441.1"
                     /translation="MAKGCKYGTHRVIEPEGAMPQAAWKIDNEMAVRDNEILINVQTL
                     NIDAASFTQIKKQAGNDEKRIGEIMMGIVADRGKHHNPVTGSGGMLIGTVEKIGPDLI
                     GSTDLEVGDRIATLVSLTLTPLVIDKIIKVHLDTDQVDIEGHAILFESGIYAKLPNDM
                     PDSLSLAVLDVAGAPAQTAKLVHPGDTVLIIGAGGKSGLLCMYEAKKRAGVTGKVIAL
                     AHSEASKARAEATGFADVVIAGDATRPLEIMKMVEEATGGKMADVTINCVNIPGTEMS
                     SILSTRDMGIIYFFSMATSFTAAALGAEGVGRDVTMIIGNGYTRGHAEIALETLRESP
                     KLRKIFEDLYAR"
     gene            complement(14712..16241)
                     /locus_tag="PLU15_05660"
     CDS             complement(14712..16241)
                     /locus_tag="PLU15_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOO30442.1"
                     /translation="MRDASIDLEHLSADLRVVLDSINEGIHIVDTCGITVFYNRIAAS
                     LDNLDPQEVVGRHILSVFPSLSEDTSTLMSVLRTGRPAPVRQQTFRNYKGDTITTMNS
                     TLPIYSGAKLIGAIEVSKDVTLVKELVERVVDLQAELITTAGVHSATGHRVKVGTARY
                     TFDDIVAVSQNMQDVVARAKRASQSGSAVLIYGETGTGKELVVQSIHNSSPRAARPFI
                     AQNCAALPETLLEGILFGTARGAFTGAENRPGLFEVADGGSLFLDEVDAMPLPLQAKL
                     LRVLQEGSLRRIGESSERKVDVRLISAMSSNPATAVERNLIRRDLFFRLGVIIIEIPP
                     LRKRKDDIPALTEHFIERYSSQFGVNVHGVTRDVFELFMEYPWPGNVRELQHAIEAAV
                     QLSHGSLIGLEHLPPHVLSHGPGDAASRPRANRFLPTSQFRDNSTPAIEEEAKAPLVP
                     TLEQIEKSLIWAALETCEGNISATARYLDIPRQTLQHKLKRYSIDASDIRRKDLDYTD
                     K"
     gene            complement(16465..17382)
                     /locus_tag="PLU15_05665"
     CDS             complement(16465..17382)
                     /locus_tag="PLU15_05665"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOO30443.1"
                     /translation="MKVAVLMGGSSSEREISLKSGAQVEAALRRLGHEVISVDPADNA
                     IGVLAELRPDVAFIALHGKYGEDGCIQGALELMGIPYTGSSVLSSAIAMDKTISKALF
                     EVSGIPTPPYVVVDCHSPFQSALERALSIGIPCVVKPSSQGSTVGISIVKDDGALQDA
                     LSYAFNYDEEAVVEKFIKGTEVTVGVLGNHDTEALPVLEIVPKNEFYDWESKYTPGMS
                     EHVIPARIGNAAQKECQRLALAAHAALKCRGYSRVDMIVEPNGTVWVLEVNTLPGLTE
                     VSLLPDAARARGISFDQLIQILLDGALKD"
     gene            complement(17448..17663)
                     /locus_tag="PLU15_05670"
     CDS             complement(17448..17663)
                     /locus_tag="PLU15_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30444.1"
                     /translation="MAALLMIGSVIAVVILVVVLWFKRSADMSDAQWRHAAIARGFLL
                     VCGIAYLLIVIKTTTCTLREIFRVWPK"
     gene            complement(17694..18443)
                     /gene="surE"
                     /locus_tag="PLU15_05675"
     CDS             complement(17694..18443)
                     /gene="surE"
                     /locus_tag="PLU15_05675"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOO30445.1"
                     /translation="MISNDDGIWAPGLLALARAIAEIADKLWICAPDRERSAISHALT
                     MGLPLRAEKTAFRGIEAEAWSVSGTPSDCVKLALEELLPERPDIVLSGINRGPNLGTD
                     VIYSGTVAAAAEGAFAGIPAIAVSTGSYDPSDYEPAARVAAKLASQVATRGLPEGVVL
                     NVNVPDGFNSGKVAVTRLGVQRYNNIFERRVDPRGRPYYWLSGDPDTPPSDDGLDTEA
                     IRTGAVSITPLRFDATCRRTADMLKEWDIRL"
     gene            complement(18484..19170)
                     /locus_tag="PLU15_05680"
     CDS             complement(18484..19170)
                     /locus_tag="PLU15_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOO30446.1"
                     /translation="MDRALLWQQLGLMSLATLIGSVIGLERERQGKSAGLRTHSLVCL
                     GSALITIVSIHGFGPAGSGLHDPARVAAQVVSGIGFLGAGTIMREGLSIRGLTTAASL
                     WTTAGIGLAIGAGMVWPAIGATVLVQFILVIMSRVERKYFPHGEAVLKVNAIDRPGLL
                     GEIATIIGKYGMNIVETEINVDRENSIVSIDVTISSNMVGRDPQLAQADLLQHVLSLS
                     GILKAEIERF"
     gene            complement(19190..20086)
                     /locus_tag="PLU15_05685"
     CDS             complement(19190..20086)
                     /locus_tag="PLU15_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO30447.1"
                     /translation="MSTASIDSKRIEALVEELKTLEQDLTTADICCKPNVADQIASIR
                     TRLLGLLADLLATGDDATRRAAAYGLSKLEDSRCIPLLADSLSDPDEGVRVWAVEGLS
                     SCGGNAALLPLVRALRDESVYVYYSACNALKSVDRDGLWPLIEAKLCSKDITVRKRAA
                     KLVGDMGEDRAVPHLIALARDADRGVRYEAVEALRKIGDPASFDVLADCLEDDSRDVR
                     IAAIQALRIIGDIRAVEPLVAICIENEDLRIPALQALKEIAGDEAVTPVIEALDKARE
                     LVSATAEQVMGIVAESHARVVS"
     gene            complement(20138..20737)
                     /gene="plsY"
                     /locus_tag="PLU15_05690"
     CDS             complement(20138..20737)
                     /gene="plsY"
                     /locus_tag="PLU15_05690"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOO30448.1"
                     /translation="MTLSPVILLSISYVIGSIPFGLLLGTSLVGVDVRKHGSGNIGAT
                     NVLRTLGFPFGFVTFLLDASKGLAVVLLGRALGANDWVIAASGLAVVAGHNWSIFLGF
                     RGGKGIATTFGFIFAIMPPVGLILAAVWLGLVCAFRYASLGSMVGVAATPIAAYIMGY
                     PSAYVIVCAILAVLAVVRHRSNIERLLSGTENKFALRAK"
     gene            complement(20757..22070)
                     /gene="der"
                     /locus_tag="PLU15_05695"
     CDS             complement(20757..22070)
                     /gene="der"
                     /locus_tag="PLU15_05695"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOO30449.1"
                     /translation="MAKPLVAIVGRPNVGKSALFNRIIRSRSSIVDDSPGITRDRVYS
                     TAEWSGRSFAIVDTGGIEADPADDISTQTRQHAEHAMKAADVIIFVVDARSGITSDDK
                     DVAQLLRIAGKPVILAANKVDHPAHMDYEFYALGLGDPMPVSAVHGIGIGNLLDAVVQ
                     ALPPQTDEPAEKDDDPIRVALVGRPNVGKSSLANRILGEERSIVSDTPGTTRDALDTV
                     FDYDGTKYVIIDTAGIRRRGKVDPGVEKYSVLRAFAAVDRCDVAVTVIDGTSDPVVQD
                     TKIAGYAHEAGKAGVIAVNKWDMVDRDKAFARDYERKVRTMMNFMPYADVLFVSALTG
                     RGITKLLDAVRKGAMHHRRRIQTSMVNRVVQDAVLRNPPPSDKGKAVRIFYASQVSSR
                     PPLFVVWTNYPELIHFSYKRYLENAIREAFEFSGTPIIVQFRARD"
     gene            complement(22076..23428)
                     /locus_tag="PLU15_05700"
     CDS             complement(22076..23428)
                     /locus_tag="PLU15_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOO30450.1"
                     /translation="MTPIMQPRARITQVDEGSRADAAGICPGDVLVSVNGHPIRDIID
                     YEYHVSSFTTRLEIEKPDGSMLTAVFNNDYDPRLGLGFDELVFDGEKHCANKCVFCFV
                     DQLPRGMRDTLYFKDDDYRLSFLQGNFITLTNLRESDLQRIMDMRLSPLYISVHSTDD
                     VTRCRLLGRTHAFPIMPTLKRLAEARIDFHCQIVMVPGINDGTVLAASIRDLQSLWPS
                     CVSVGVVPVGLTSHRQGLPELEPVSAGIARQTLEIVDSAQNECMKLFGNHFVYAADEF
                     YCLASLPFPIADAYDEYPQIENGIGLARLFLDEFAQIALELPCSVAGRICATVVTGVL
                     GARVIRDACDSLNGIHGVKVRLVPVENKFFGESITVSGLVVGADIIRSLQEEASRGRL
                     GDFVVIPDVMLKSGERSLLDDVTVEGIASSVGVPVVVAPTTARGLVESILALHPPNAR
                     "
     gene            complement(23529..24443)
                     /locus_tag="PLU15_05705"
     CDS             complement(23529..24443)
                     /locus_tag="PLU15_05705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO30451.1"
                     /translation="MDKRKAGLVRLAIIVAAIVVAGIIWIVYTNSAHVAIVNGERISR
                     RDFIARLEEQYGEDTLAELINETLLHQAAKKAGVEVTPEEVDKEVALLKEQIGPSYDM
                     LIAQYGWTDEQIKDIIEMNLLVYKISTKDITIEEDALMAFFEENKSDYDEPEQVLASH
                     ILVATEEEAKEVKQELSEGADFAELAKEKSLDVATAVNGGDVGWFPRGRMTEEFEKTA
                     FALSPGEISGPVKTEFGYHIIKVMDRKAAREASFDEVRDDIERSLKMQQSKTAQQLVS
                     ELKLDSDITITDSKYKDLGTTTPFGIKE"
     gene            complement(24613..27015)
                     /locus_tag="PLU15_05710"
     CDS             complement(24613..27015)
                     /locus_tag="PLU15_05710"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOO30452.1"
                     /translation="MTCAPIIREAKHAGFCRGVERAVRIACEAGQEALRSGSGGAVTL
                     GPLVHNPDVVSSLLKCGVEQVDSIESASGRVLIIPSHGLPPETIQAARERGLTLVDAT
                     CPHVLRSQREVARAHSEGRYVVIVGDKHHPEIRGVAAFAAGDVVVVSDPNEAESLTLP
                     ARPITVIGQTTHKQSLFEAVVSVLRNRGAVVDAVNTICPATSERQEAARTLAGEVDLM
                     VVIGGAASANTMRLAEVCRESGARVLKVENACGLDREAILSARTIGVTAGASTPSWVI
                     EEVKDAMHEIIEGNSEEAKCLECQDEQTCEASSTELAKEREETAVEQGETVPEESAER
                     VAVQPSESTSATEECEKTETSPSEVGTDESSDDGGEEAAEGSAPPDTGGAETVDADSD
                     QEEAGDGEQVEAEEEKPATERFTPPAHELPSDPDVIPVRVKEVYDDRVVVEVEPGKDA
                     VIFKKHLSIEQISHPSEVVSVDDEIYVVLDDSQRPSSDMMYASKTRADTLLKWQTLEN
                     AMDTGEIIEGKVTEAVKGGLVVDIGVRGFVPASQVGRRFVEDLNVYVGQTLRLKVREV
                     ERLRSNVVLSHRAVLEEEERLAKERAFQTLERGQVLDGTVTRLVSFGAFVDIGDGVEG
                     LLHISDISWSRIKHPGEVLAEGQSIKVLVLNVDRERERISLGYKQLLPDPWENIGQRF
                     PVGSVVSGTVTKIVDFGAFVRLEDGIEGLVHISQLADRRVGRPDEVVSVGETVTVKVI
                     GLREQEHRISLSIRQAMEDRERNEYRRYMKESKESDVVTIADRINYDPSKLKGSTDGD
                     DR"
     gene            complement(27012..27659)
                     /gene="fsa"
                     /locus_tag="PLU15_05715"
     CDS             complement(27012..27659)
                     /gene="fsa"
                     /locus_tag="PLU15_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOO30453.1"
                     /translation="MKLFIDSANIDEIREVAEMGVVCGVTTNPSLVAKEGRDFKEVVS
                     EICRIVDGPVSAEVISQDADGMIREAAEISTWSPNIVIKIPMTSEGLKAVKVLAARGI
                     NTNVTLIFSSSQALLAARAGATYVSPFVGRLDDISSDGMNVVAETASIFDIHGIATEI
                     IAASIRHPMHVIEAALVGSDIATVPYKVLMQMAKHPLTDAGIERFLADWEQAIKK"
     gene            complement(27680..28291)
                     /locus_tag="PLU15_05720"
     CDS             complement(27680..28291)
                     /locus_tag="PLU15_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO30454.1"
                     /translation="MFYSFAKFVMTLYLSIAFGFKVTGKENVPATGPVIIVANHASIL
                     DPIALGCACDRRIRFMAKAELFENPILAAVLRWLYAFPVRRGRVDREAYRQSMDILKA
                     GGALGMFPEGTRSETGELQKAYPGAARFAIQTGTPVLPAAVVGTFAGHRKGLKRFKEP
                     MVRVVFGELIWPKVDRAGATVREETDALAEEIMKRIGELIMIA"
     gene            complement(28264..28965)
                     /gene="cmk"
                     /locus_tag="PLU15_05725"
     CDS             complement(28264..28965)
                     /gene="cmk"
                     /locus_tag="PLU15_05725"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOO30455.1"
                     /translation="MSLEHSIAIDGPAGAGKSTVARMLAHALGYRYVSTGDLYRALAL
                     EVIRRGLDPYDEAAVVDVAKSVCIRVERSECGGQRTILNNEDVTEEIRSAEVDSVVSP
                     VSVYSQVRKHMVEAQRAIAYDADVVMDGRDIGTVVLAESAHKFYLTASAEERARRRQL
                     DYARQGRHVDLADLVEEIEKRDTIDSTRQISPLRPAPDAVVIDCTTLSPDEVVDTMLA
                     ALRRPASCFTLSQNS"
     gene            complement(29064..29612)
                     /locus_tag="PLU15_05730"
     CDS             complement(29064..29612)
                     /locus_tag="PLU15_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30456.1"
                     /translation="MQNWDVATIVFSALTGLALSACILVQVLLSCGVIPRSVLMIERI
                     EGHPSWSWTPSPGKSGDSGKPDVRAGAAVEAGGILLKLSALDNGLECAEPTLTVLVNG
                     KPVADFKYGSAAVVIHRGDLLEVEVNNADEDRIDSTAGGAHVEQLEIEVEDVTPNVTK
                     PQKGDKCSIGPGRTQICNVIII"
     gene            complement(29617..30879)
                     /locus_tag="PLU15_05735"
     CDS             complement(29617..30879)
                     /locus_tag="PLU15_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO30457.1"
                     /translation="MDVAIIGAGASGLMAAAAAAGAGADVVVYERNRIPGKKLLISGK
                     GRCNITNDSPVADIVEAFGPGGRFLYSAMSNFGPEELQALLLSLGVECKVERGKRVFP
                     VSDSAADVQNALYRHATNQGARFAFGRQITGLAVADGKVKGVIDSGGNNSWADAVIIA
                     TGGISYPGTGSTGDGYRIAAEAGHTIVQPLPALVPMETVEDWPGSVAGLSLRNVELSL
                     YDRGRRIGREFGEMLFTHFGVSGPIVLSLSRAVCRSLRESEAIESGRFTLSIDLKPAL
                     DEATLHRRIQRDLVAASKKQLKNSLGMLLPHSIIPVVISLAQVDETIPANQITAAQRS
                     ALVETLKRMPLTVLKTRPIEEAIVTSGGIALREVDPRTLESKLLPGLYFCGEVLDIDA
                     PTGGYNLQAAFSTGRLAGMSAAMSRRSG"
     gene            complement(30918..>31319)
                     /locus_tag="PLU15_05740"
     CDS             complement(30918..>31319)
                     /locus_tag="PLU15_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30458.1"
                     /translation="LKIIGDHALKLYVDGNFTWNGSRGGAPPLDPTKFVIYCTGPKNP
                     RDFPPNVTMNGFPNMAAVIYAPRADVVFNGKGEFSGAIVGSSITYNGSGRLAFPTGVY
                     ADKLKSINLGEVDGPPRSVPGSWRELPIGER"
BASE COUNT         6771 a         9131 c         7777 g         7640 t
ORIGIN      
        1 aaagccaacc aacctcatct tacgaggata tccggtcgcg ctatctcact gccgtcattg
       61 ccttgcattg aacgcatcgg ccaattcccg gtttgcgtta tgatgtcggg gccgtccttc
      121 gtggcgatga tcgtgtcttc gcttcttatg ccatggacga tcgggttcca agcgaaagcc
      181 tggttttcca ggaccgtctc ttgactggag cctgttgcta ggtagtcccg aaccagatat
      241 cccgtggctc cgcccaagtg gtgctttttc cattcgtcag gatagccaac gtcctcatag
      301 gcccggacgg cggcatcaaa acacttcgag actggttgac ctggacgcgt tgcacagatt
      361 gcggctgctt cgatccggat tgccgcatca tgccatgctc tgatgtgatc tgggatcggc
      421 cctatgcaaa ccatccttgt ggcgctcgca gatagccccc atctactgcc agcaactgtt
      481 aacatggcgt atttttccat gcgggagtag gtcggggtgg gttggctatt gctcgtattc
      541 cttccatctg tcgcaataag tgtaacccgc ggagcgattc ctgaatcaac cagccgcact
      601 gcaagaagag aagagatttt atgttcagtc atgccgggtt ccacacaacg cgctacttct
      661 tccaaagctt tgcccagctg acctccgagc cacctgtatc ggtcgatctc ctcgggttcg
      721 agtttgaatc tgaggggcgc aacttcatcg ccgtagtaga ttgcgccgct catcaggaca
      781 tcggcgccta cagcgttcct cgcgccgaaa ctgtctgggt caggcagccc ctcataccat
      841 gacctggaga tcagcttgaa ccccttttgt tctaggcacg aattctggtt gagccttgcg
      901 atttcggcct catccgctgc tacatacgct gaatcctcag taaccacggc gcaggcagct
      961 acgcgtccag ttgtctcatc caccgaaggc gcctcgcctc cggtgatcca ggcgaagttt
     1021 gaacgctcca cgagcagcat ggcgtctagg cccctttcta acatgagccg acgcactttc
     1081 tccaatttgt tttgaacttc ccgttccatg aacatcaccc ctgctcagtg gctacatgtg
     1141 ccacatgatc caccagcgca cgaaccacat gatccgcctg aagacgacga aaacgtcgat
     1201 ccgctcttga agccgaaaga ggagaagagt ttcttaacct cgtcgctccc acactttgga
     1261 catttgacat cggcacaaca accagtaact agctcttcaa atttctcacc gcatttggcg
     1321 cacttatact cgaatattgg catttgtgtc cctcctagtc atgccatgcc aagaagtgac
     1381 aacatggtct tggcatttac cgcggcttgc ccggcgtggc aattattgaa caaaacgaaa
     1441 gcacgcttcg tggcctctgc gagacgctgt atttttggcg cccactcaag gagctctgct
     1501 tcggtgtata gataattata acgttcccaa gcttcattat gtttccacca cgtcttagca
     1561 tttctcccgt gaaaccttac ataagcaatg ggagacgtat atacggcaag aggtggaaag
     1621 agcccccgta ggttgggttc atccacacag caaaacccca ttcccgcccc tttgagcagg
     1681 tcgaacgtag actgggatac ccattccaca ttcctgaact cgaccacgat ggggatatca
     1741 ggcagcagtg caggaaggcg ctggacataa ctgctatttt cgggagtcct cttaaagccc
     1801 catggaaact ggcagagaac acaacccagc ttgccttcac tgactaaagg tgccatcgct
     1861 ttggagaatg tctggaatgc ctctgcgata tcattgctgt cacgagaaag ctcatgagtc
     1921 atacttcgat gtgcctttac gcaaaagctg aaattctcgg gagtcctgcg agacatgcct
     1981 ccaatggtct tcgcatccgg catgcgatag tatgtgaagt ccagctccac cacgctgaat
     2041 tgagttgcat accattcgag catctcccga tcggaaatgc tttctgggta gaaaacgcct
     2101 cgccaatccc tgtagctgaa cccacaagtt ccaacctcta tcacggcgaa gatccctcca
     2161 tcagcttgaa gacaagctct atgaatccat ccttgaacga gagttctgcc ggaggtttcc
     2221 cgaaggactt cacctctgct atttcttgga catcacttgg ctcgcccaca gcaagcacat
     2281 aagcaatgta tacaatcccc ttggaactgt ttaccccgtt ggtcactgtg taccagcaca
     2341 gaggcttcaa gtcgtctacc agagcccccg cttcctccaa cagctcgcga cgggcggcct
     2401 cttcagggct ttccccctgc tctatttgtc ctccgggaaa ctcccaacct gcgcggcgga
     2461 catttcgaac cagaaggagc tccccgtctg ttacaggcac gaccaagaca ctgtcaggtt
     2521 ttgaccattc atcctcagga ggacgaccac taaaccaaag atctgtatgg actccgggcc
     2581 cgtccagctt gatgaagcct ctttcgtcgg ccttgtcagt cttccaactc cctacctcga
     2641 atcacaccct tcgacccata cctttgagaa agctcgtcca acacgtcgtc aatcgcacag
     2701 ttccgattgt ccgatccgaa aagcgaaagc tgccgacaat cggatagccc ggatacagat
     2761 aggccgataa gcctgacagc catgcccata tcaatttcgt ccagcaaagt acatgcagtg
     2821 ttgtagatgg tatcgtaatc atcgaagggt tcagccatgg tcttgctcct cgtcacttgg
     2881 ctgaagtctg caaacttcac tttaattgtc acggttcgcg cgagtttccc cgctccgcgc
     2941 agccttgacc caaccctgcg ggcatgttgc gccaggtatt gcacaagttc gtaccggtct
     3001 gcgatatcct cgtcgaatgt cgattcagac ccgaaggatt tctgctcctc atctgcaacc
     3061 actgggtcat catcgatccc gcgagccaaa tcgtagagct gtgctcccca ttttccatac
     3121 tgctctacaa gccattcctt tgaccagagg cgaagatcct ctacggtttc aatgccgtat
     3181 cgtgcgagag acctctcccc cttttcgccc actccccata catctcgtac tgggagagga
     3241 gagagaaacg cgtccacatc ttctggacag atcaccaaga gcccgtctgg cttggacttc
     3301 gatgaagcca gtttggccaa gaacttgttt tgtgctatgc ctacagacgc cgtaagcccc
     3361 gatgcatcct ttatggcttg ttttatctgc gtgcccagat ccaaggcgtc tgtgtagaag
     3421 tgttcacacc cggtcatgtc gagaaatgcc tcgtctatgg agacctgttg catgatgggc
     3481 gtgaacgcct ctaagacacg cattatttcc cttgacacct gcacatatcg ggccatgttg
     3541 cccggcacga aaatggcgtg agggcacaac cgcgctgcct gtgacatcgg catcgccgac
     3601 ctcacaccat aggcgcgcgc ttcataggaa cacgtagaca ccacagcccg aacgctgtca
     3661 cgaggcccgc caacgataac gggtttcccg cggtaatcgg ggttgtcaag tgcctctact
     3721 gccgcgaaga aacagtccat gtcgacatgg gcgatggtac gtgtccacac acgaaatcac
     3781 tcaacttttt ttattatggt taccaggtcg ccgttgccta ggcaggccgc gttggcttca
     3841 tcggtgtcga tatgaaactc ccacgtgtag gaatcatcga cacgtacaag aacttcatca
     3901 aaaatgagtg ctcgagcccc attgaccttg gtcattacaa tgtctttatc ctttaacccg
     3961 atgcgctcgg cctcctcagg agtcatgtgg atgtgcctct tggccactat gacgccgact
     4021 ggaatagtga tctcggcttt cgggccaatg agcttgattc caggcgaacc gtccaggtca
     4081 cctgagtcgc gcaccggagg ttgcacgcca agcttgaaag agtcggtgcg ggaaatctcg
     4141 acctgagtcc gtttgcgaac tggaccgaga acgcgaactc ttgaaatagt gcctttgggg
     4201 ccaacaacat cgacagtctc ctcagaagca tattgccctc tctggctcag atccttcctg
     4261 actgtcagct cgtagccttc gccaaacaac gtatccaaat ccgcacggga aagatggaga
     4321 tgacgattgg atactccaac aggaacctcg tataatgaca ttacccctgc ctcctatgtc
     4381 aatgcgatac acgattacat gatagcctgt gtagccagca aaagcaattg aggccggcca
     4441 gcaaagggtg ttacaacacg ctacctcttt cgataacgct cctataccac ctggcactgg
     4501 attttgggat cctcttgaga gtagtatagt caacgtagat tatcccaaac ctcttggaat
     4561 agccgaaagc ccattcgaag ttgtcaagta ttgaccagag atagtatcct tccacctcca
     4621 caccgtcttg catcgcccta tgcaattgga taaggtgggc gcggatatac tccatgcgtt
     4681 ctcggtcgtc gactaagcct ccagagctta cctgatcgtc gtaggcagcc ccgttttccg
     4741 tgataaagac ggcgggagga tcgtagtctt gctcgatccg gagcaagagg tcgtacagtc
     4801 cttcaggata aacctcccaa cccattgcgg ttacaggcct gttcgtcctt acatgagaga
     4861 gttcgagagg ctgtgcctct gggtcggctt ttaccacggc cctgctgtaa tagttgaccc
     4921 ccaagaaatc gattggagtc gagatgattc ccatgtcttc aatacttata gatggtgcac
     4981 cgaaggcccg caaataaagc tccatgatat cttctggata tgagcctaga aagagcggat
     5041 ccgcatacca ccggttcaga cagccgtccc accgtcgtgc ggccaatacg tcgcactcat
     5101 tggaagatgc gggatagacc ggactcatgt tcagggttat ccctatgcgg gcaccttgcc
     5161 gggaaaggtc cctgaaagcc ctcacagctt ctccatgcga tagaaggata ttgtgagctg
     5221 ccgcaagggc tgacggcatg tgggagacgc cgggagcgtg ctctcctgtc ccgtagccca
     5281 aggttgcagc cacccatggt tcgttgtgtg ttatccagag atcaactcta tctcccaagg
     5341 cttcaaacat gtaggctgcg taatcaacaa agtattttgc tgtgtcgcgg gctatccacc
     5401 ctcccttatc ctgtaaagcc tggggaaggt cccaatggta cagagtcgcc atcggcctta
     5461 tgccggctgc aagcaactcg tctacaagcc tattgtagaa atcgacgcct tttacgttcg
     5521 ggcgtccgcc tccgacgggg aatatccttg gccatgcaat ggagaagcga tatgaattga
     5581 gcttcaatga ttccatcaag gagacatcct ctgagaatct atgataatga tcgcatgcga
     5641 tggccggagt gtctccgttc acgatcttcc ccgggatgcg acaaaacctg tcccatatgg
     5701 attcgccttt gccgtcttct agcgtagcac cttcaatctg gcatgaagac gatgctgctc
     5761 cccagagaaa accatcaggg aatgtgtgta gcgtcaagtt gcatcaccta ctttcagcga
     5821 taattccaaa cttcaacaca gggcgacgaa tccctccgac cccagcgagg ttggagatgc
     5881 atattacttc aaccgcagat ttgggcggaa gctccgggca cggtcacggt catcccgcgc
     5941 cttgacaatg gctctgatgg gttgtagaat ctatttagtc gcaagatgaa gtcttttcgc
     6001 aggggagtag ctcaattggt agagcatcgg tctccaaaac cgagggctgc gggttcgagt
     6061 cctgtctccc ctgccattat ttcaaaagcc acctggctaa gctcaggtgg cttttgcttt
     6121 tgttcgtgtg cttaggcgtg cgccctaaac aacggtcatg actcctactt catccggaat
     6181 atacagcctt gcttctgttg cagccaggac ctctcctatc gtcacgtctg gcgcaatttc
     6241 gcgcagcacc gccccttcag gggtaaactc tatgacgcag agttccgtga cgagcaggtc
     6301 cacttcggct gtggcggtca atggcaaagt gcatttgcgc accactttcg atcggccgtc
     6361 tttggtccta tgttccattg ctacgatgac tcgctttgcg cctactacga gatccattgc
     6421 gccgcccatt ccgattatcc gtcccggaac ggcccaattg gccagatttc cgtcctgatc
     6481 cacttgtaac ccgccgagga ttgttacatc cacgtgccct ccgcggacta tcgcaaagga
     6541 cgttgctgaa tcgaagaatg ccccgccagg ctttatggaa gaaggttgtc cgccggcgtt
     6601 cacaagatcc gggtcttcac gacccggttc cggaggatct cccatgccta caaagccgtt
     6661 ctctgagtga aggataatgt tcacgtcgtg agcaagatgg ttcacgacga aggtcggcag
     6721 gccgattcca acgttcacga cctgtccgga cttgagttct tgtgctactc tccgtacgat
     6781 aagctctcga ggttctattg ccgccacttc agctcacccc tacaactgcg tctacgaata
     6841 tgccaggggt catcacgtga tctggatcga taccgcctat atcacggatc tcgctggcct
     6901 ccgctacgac gtagtcagct gccatagcca tgagcggatt gaagtttcga gccgatttgc
     6961 gatacaccaa gttgcctgcg cggtctgcca cgtgtgctcg aagaagtgcg atgtctgctc
     7021 tcagtggaag ctctagaaga tatgtatgac cttccaactc cagcttgatc ttcccttctt
     7081 cggctatggt tcccacgcct gtaggcgtca gtatgccccc aagtccggct cctccggcgc
     7141 gtattctctc tgccagtgag ccttggggac acaattccac cgcgagtttt ccctcgtctt
     7201 tctgagcgat tgcagtgggg ttcataccca tgtagcagca gatgagcgta gatacttgcc
     7261 cgttggcgaa tagtctcccg tatccccgat caggcatcgc tgtgtcattc acaatcaatg
     7321 taagatcgcg gactcccctt cgtatcacct cgtctacgag cgtctctggc gagcccaccg
     7381 ccagaaagcc accgatcatc agagtcatgc cgtcggtaag gccttcgagc gcttggtcaa
     7441 gcgttctcag ctttggcacg cttagcactc acctccgtta tttcttgagc tccagcatct
     7501 ccctggcttc gtccggagtg gcaacgtccc tgcctagttc acctgcaatt cgagcaattc
     7561 tggcaacaag ctgggcattg ctttttgcga ggacaccacg ggaatagtag atgttgtcct
     7621 caaaacccac tcgcacgttg ccgcctagca ctatggcaac agctgcaagc tgaagttcag
     7681 ccctgcctat gccagcaacc tgccacatgg ttccaggcgg gagactgttg acgcaatgca
     7741 tcaggtcggg cacggttcca ggtatgcctc ctggcacgcc cataacgaaa tcaaaataca
     7801 ggggttcttc tacaatactt cgtttggcaa gtcgaagtgc gttcgctatg tgcccaacat
     7861 cgaagcactc cagttctggc ttgacccctt tttccttcat cctgaccgcc agggcctcga
     7921 ttgcctgtgg ttcgttcatg aatacctcat caccgaagtt gactgtgcct gttgtgagcg
     7981 tagccatctc aggagcgagg tcaaggggac gcattcgttc ctcaatcgga gttcctactg
     8041 cgcctccagt cgagatctgc acgattacgt ctgttcgttt tttgatctct tctattgtct
     8101 tgcggaatac ctcaatgtcc tgggttggct tgccttcctc gtctctcacg tgcagatgga
     8161 cgattgaggc ccccgcattc ctggcctcct ctgcggcttt gccgatctct gcggggctga
     8221 gtggaaggta aggcgcctgt tcccttgaaa gttccgcccc tactagggct gcagtgataa
     8281 tgagtttgtc caattgatta gccctccttc ttagtggtct ccgcgtcctg cccacagccg
     8341 cagcaattgc cttgcttgcg ttgcttatcc ttcgggacaa cacaggtccc actggcgcgg
     8401 gcaacaagga gaggctcctc cagaataaag gctgctgaag gatcctttgg atcacggcat
     8461 gcttctatga ctttgtgggc ttcaaaggtc attgcccgag aagtattgcc cgttctcaca
     8521 atgcgtccgc gggcttcgat atagtcgccc gcatagacag gggccttaaa ctccaccatg
     8581 tcatatgcga cgaaaagccc ctcatctccg tcgtgcctta taaggagttc agtggcgacg
     8641 tcgccgaaca gctgcattag cttggcgccg tccaccagat ctccgccgta atgggcgtca
     8701 gactgactca tcctgaccct gatcatgact tcttccatgg tattctcctt tcgtccactc
     8761 ccctcacctt tcttccgtca ggcgcgcgga agtgcggtgc tcctttctta ccaattcctg
     8821 agctatgtac gcggcaacgt gagacggcaa tgtgccaggc ccgaatcccg cgtcgaatcc
     8881 gagctctagg gcgagttcat ggctgattcg cggaccgccg caaatcagga gcacatcatc
     8941 ccttatacct tcagcttcga ccaagtctac aaggcgcgtg aggtttgcta tatgcacgtt
     9001 cttctgcgtc acaatctgag aaactaggat ggcgtcagcg cctgtttcta tagccctggc
     9061 aaccaaggtc tcattgggaa cctgggcccc caggttgtat gctcggaacc agggataccg
     9121 ttccagtccg tattcgcctg catagccctt catattgatt atggcatcga ttccgacggt
     9181 gtgggcgtca gaacctgtac acgcggcgac tactgtaatc ttgcgaccaa gcttgtctct
     9241 gatgaaatcg tttatctgga aataatccat ctgttcgact tctactttgg gtatgtgaag
     9301 ctgtgtgacg tccaccgagt gcacgcattt gccatatacg acaaagaagg tgaatggacc
     9361 tccaaggtcg tgggcgctca cgacgtccac atcctcaaac cccatcttca tcgccagctg
     9421 gcgcgctgct tcttttgcct caggcccgta cctcaagggg aggctgaatg caagttgcac
     9481 ttttccgtca tcaagggtgt cgccgtatgg tctgaccctt tccaaatcta ctttcggcac
     9541 gaactacacc tcccacccct tgtgagccgg gccggtatct cttccgcaaa tgggttccag
     9601 tattctggat ttctttccac gactccgtcg agtcctctgc ctccatcggg cgtccgggag
     9661 ataccggcaa ataccccatc ttctatagcc gccatcaatc ctgtcttccg cacttgctct
     9721 aatgttgttg tagcttcgtt gagaacctgt tgagctcggc tttccacgat tccgccacgc
     9781 ttgaaggtta tctcttcgcc cagattgcgt gcgttgttga agacgtattt tgcgttgcgg
     9841 attgcaaggt atctgtcgtg tatgtgcgga gtgtgaacgg cttcggtgag catgccgaga
     9901 agctgtatgg actggcctgt catcactcct gccaagttga acataccgtt catgagatac
     9961 cctttgaata tgtcgcctgt catgtatttt gtgggcggca tgtatttaag cggtttgttg
    10021 gggaatatgt cccgggccat ctgcgcttgc gccacttcgt agagaaaccc gttagttgtg
    10081 gccgggtcga tctcgaatgc atgccccagg ccgatctgct cgtcaggcaa gcctgatcgg
    10141 taagcgagcg cctcattaat gaactgcgac gccagcacga tgtgggcctc ttcataagcg
    10201 tctgaagtag taagatagtt gtcctctccg gtgtttatgg ttatgccggc gtaggcattg
    10261 agcatacgcg aaaagtgctg gtctgcaaag gtgcgcttca tgttgatgtc gcggaagagt
    10321 atgccgtaca ttgagtcgtt gagcatcatg tcaaggcgct ccattgctcc catggccgct
    10381 atctcgggca tgcacagtcc tgaacagtag ttgacgaggc ggatgtacct gcctgtctcg
    10441 aggcttacct cgtcgagagc ctttcgcatg atgcggaagt tctcctgggt agcaaatgtt
    10501 ccgccaaacc cctctgttgt ggcgccatat ggcacgtaat cgagcaagct ctgggcggtg
    10561 cttcgtatca ccgcgattat gtctgcgcct gatctggcgg cagattgcgc ctggccaaca
    10621 tcctcataga tgttccctgt cgccactatg acgtatatga gcggctcagt tccagggtca
    10681 agcttctcaa tgagctcgca acgctttgcg gcgttcgcct ctattctggc aaggcttgct
    10741 tcaattgcgt cttccaggac tgctctagcc cgatcgtcct cgacgtcatc gaaactggcg
    10801 aagtcgagct cgccggcggc agctttgtcg gcgatggcct gcggagaaag gcctgttgcc
    10861 gccacggccc tccccatatg gcaagcggct ccgtctccta gtctccctgc ttcgcgcaac
    10921 atatcgacta agacattggg aagcgggacc cctgcggaat cgacgccgtc aacgccgact
    10981 aggcgcaaga ctgtacgctc tatggcagtc gtagttacac gctctacacc tttcattacc
    11041 tcgtctgcta tgtcgcttgc acattccctg catcgcgcta tcttatcagt atccaggttg
    11101 agtttcaaaa gccacacctc ccactgccga gagccccgca accacgtcaa ttacagggag
    11161 ctctctgata tgtcttgaga tctcgagtag cagatctgct ggatctaaag gcgctctcca
    11221 cggggacgtg gggtttatgc tgatcgcgat cactggcgcc ttctcgagaa cacacagctg
    11281 tcccccggat gcctcgaatg cctctgctcc ttggtttccg actgccaacc gcgagggatc
    11341 cttggcaacg accataaatg cctgcttttt tggggacaac ggttccttag gcccgctggc
    11401 cgtgtctggc ggcgcggctc tgcgtctctg attgagcctc gaggcgacct cagccagaag
    11461 aacatcgccc accgccctcc cgcatatgag ggcaacatcg tcttctgcga tggccgaggc
    11521 aatctcctct tcccttccca ggattgtctt ccatggaaag acctcaatct ctccatctgc
    11581 ccgaagtatt gccgagactc cttcttgcat tatgggctct gcaacttgca ccacgtctgc
    11641 cggaggccgg ggcaactgga acatgtgaac agcgcgctcc gtcaattcgg caatcctatt
    11701 tgccgaaccg tttagagccg cccctgtggc caggacgaag gccccgacta tgtcgggcga
    11761 tgcgaggaat gttcttcccg ccgctccgtc caccaagatg agctctgcgc cgaattctcg
    11821 cattcttcta acagctagca tcaggtcgtt cgaatggttg caccctatca cttcgacatg
    11881 gcccggggac agaaccctcg ccaggacgat ttcgccagca ggtgtccagg ccccgagccg
    11941 ctccaccacg tccagactac acgaacctgt atgaattgta gattcggcaa gagcaacgag
    12001 agatcccgcg ggcagatata ttgggggttt cggaagtcct gttatggcat catacagttc
    12061 gccatctcgc ccgccggacg tgacgcctac cgtgatgccg ttggatgtcg cctgtcggat
    12121 catcctgttg agcacggtcg ttttgccgca attctttgca aggccgacta tgccgacgca
    12181 tttgcccggg gcccccagag caaggtcaag aaggatctct cctggatctc ttgcgacgct
    12241 cgagtccaac tggttcaagg cttgacacca tcccatccat aacagccgcg acgccctctg
    12301 tagcgggctt cggaatctct tcgcagatgc cgcactgatg gcactggctg atcttgtctt
    12361 cgggttcggg ataagcggtg atgacgcctt cgtagtttcg aagaattact ttggtgtcgg
    12421 tctgcgagat gacatattgc ggggcgatgg ggatctttcc tccccctccc ggggcatcta
    12481 tcacgaaagt gggaaccgcg aatcccgatg tatggcccct caaacgctct acgatctcta
    12541 tccctttgcc taccgatgtc ctgaagtgct ctatccccat cgatagatcg cattgataga
    12601 tatagtaagg gcgtactctg atcttcacaa gttcgtgcac gagcttcttc attatatggg
    12661 ggcaatcgtt cactcccttg agcagaactg tctgattccc aaggggtatg cctgcgtcag
    12721 ccaacatctc gcacgctctg cgtgactcag gcgttatctc cttcggatga ttgaaatggg
    12781 tattgagcca caggggatgg tacttcttga gcatattgca gagctctggt gttatacgct
    12841 gcggcatgac gaccggggtt cttgtgccga tcctgactat ctccacgtgg gggatcgccc
    12901 tcagctcgct tatgatgtac tctagggtgt catctggtag ggtgagcgga tctccccctg
    12961 aaatcaggac atcccttatt tcaggcgtct cccttatgta ctggatgccc tggatcaatt
    13021 cgtcatgggt tatggcgctg tcggtatgcc cggcaaacct cctcctggtg caatgcctac
    13081 agtaggttga acaaacggac gtgacaagga acagaaccct atcgggatat ctgtgagtta
    13141 cgtgaggcac aggggaatcc acatcttcgt caagtggatc catgagatca tgctctccca
    13201 tggaaagctc ctggacgact ggaattgcct gttttcgaac tggacaatca gggtcgtcag
    13261 gatcaattaa actcgcgtag tgcggagtta ttgccatgcg taacctggca aggcagcgct
    13321 gcaccccttc ttcctcttca ggggtgagat tgactaccga cttgagctgg tcaacagtgg
    13381 ttattctgtt tccgaactgc cagtgccaat cgttccactg ttccttggaa actccttccc
    13441 acatactgac atcatctccg tcgttatctt gcatagagat cctcgaatat cttgcgaagt
    13501 ttcgggctct cacgcagagt ctcaagggca atttccgcgt gtccgcgggt atagccgttc
    13561 cctatgatca tcgtgacgtc acggccgact ccctctgcgc ctagggccgc ggcggtgaag
    13621 ctcgtcgcca tgctgaagaa atatatgatg cccatatcgc gtgttgacag tatggacgac
    13681 atctctgtgc ccggaatgtt aacgcagttg atcgtgacgt ccgccatttt tccgcctgtc
    13741 gcctcttcaa ccatcttcat gatctccagc ggccgcgtag catcgccagc aatcacaaca
    13801 tcggcaaatc ctgttgcctc ggcccgggct ttactggctt cgctgtgtgc caatgctatt
    13861 acctttccgg tgacgccggc acgtttcttg gcctcataca tgcagagaag gccggattta
    13921 ccgcctgccc cgataatgag cacggtatcg ccaggatgca ctagctttgc ggtctgtgcg
    13981 ggggctcccg ccacatccaa aactgccaac gagaggctat ctggcatgtc attcggaagc
    14041 ttggcataga ttccgctttc gaaaaggatt gcatgccctt caatgtcaac ttggtcggta
    14101 tcaagatgaa ctttgatgat tttgtcgatg acaaggggag tcagagtgag agagacgagg
    14161 gtagcgatcc tgtctccaac ctcaagatcc gtactgccaa taagatcggg tccgatcttc
    14221 tcaacagttc ctatcagcat tcctcctgaa ccagtaactg ggttgtggtg cttccctcgg
    14281 tcagcgacga tgcccatcat gatttcgcct atccgtttct cgtcatttcc ggcctgcttc
    14341 ttgatctggg tgaagctggc agcgtcgata ttgagggttt gaacattaat gaggatctcg
    14401 ttatcgcgga ctgccatctc attgtcgatc ttccatgcag cctgcggcat cgcgccctct
    14461 ggttcgatta ctcggtgggt tccatactta cagccttttg ccaattgcca cacctcctat
    14521 gatacacgca cacaagagat aacgttgcaa tatccatgcc agagacgaaa cacgctgtag
    14581 ctgcgcttgc ccctgtccaa cgtctgctca tttacgggca gctgcccaat tatgggcact
    14641 gcgtgcgtga cagttcgcca cttcaagtga catatagaac tgccccggcc gctccaatga
    14701 cggaccgtta cttatttgtc tgtataatcg agatcttttc tgcgtatgtc ggaagcgtcg
    14761 atgctgtaac gcttcagctt gtgctgcaga gtctgcctgg gtatgtcgag gtatcgggct
    14821 gtggcagaga tgtttccctc gcatgtttcg agtgcagccc atatcaggct cttctcgatc
    14881 tgttcaaggg tgggtaccag cggcgccttg gcctcttcct ctattgccgg cgttgagtta
    14941 tctcggaact ggctggtggg caggaatcta ttggctctag gtcgggatgc cgcatctcct
    15001 gggccgtgcg aaagaacatg cggtggaaga tgttctagtc ctatgagtga tccgtgggac
    15061 aactggactg ctgcttcaat tgcgtgttga agctctctca catttccagg ccacggatat
    15121 tccatgaaca gttcaaatac atcacgggtt actccgtgca cgttcacgcc aaattgtgag
    15181 ctgtagcgct caatgaagtg ttcggtaagc gcaggtatat cgtcttttcg cttcctcaga
    15241 ggaggtatct ctatgatgat gacgcccagc ctgaagaaga ggtcgcgtcg tatcagattg
    15301 cgttctactg ctgtggcggg attcgaggac atggcggata ttaggcgcac gtctaccttt
    15361 cgctcagaag actcccctat cctgcgcaat gagccctcct gcagcaccct gagaagcttc
    15421 gcctggagcg gcagaggcat tgcgtcaacc tcgtcgagaa acagacttcc tccgtctgcg
    15481 acttcgaaaa gccccggccg attctctgcc ccggtgaagg cgcccctcgc tgtcccgaaa
    15541 agtatccctt cgagcagggt ctccgggagt gcggcgcagt tctgtgctat gaatggccgg
    15601 gccgctcgtg ggctggagtt gtggatcgat tgtacgacga gttcttttcc ggttccggtc
    15661 tcgccgtaga tcagcactgc ggaaccgctc tgagatgctc gctttgcacg ggccacgaca
    15721 tcctgcatgt tctggctgac agcgactata tcatcaaagg tgtatctggc cgttcctacc
    15781 ttgactcgat ggccagtcgc cgagtgcacg ccagctgttg ttatcagctc cgcctggaga
    15841 tcaactacgc gttctactag ctctttcacc aaggtaacgt ctttggagac ctcgatagcg
    15901 cctatgagct tggcaccgct gtagatcgga agtgttgagt tcattgtcgt gatggtgtca
    15961 cccttgtagt tccggaatgt ctgctgcctt acaggggctg ggcgaccagt acgaaggact
    16021 gacatcaagg tgcttgtatc ttctgataat gatggaaaga cggatagaat atggcgtcca
    16081 acaacttctt gggggtctag gttgtcgagg gaagctgcaa tcctgttgta aaagactgtg
    16141 atgccacatg tgtcaacgat gtggattcct tcattgatgg aatcgagaac gactctcaga
    16201 tccgcactga ggtgttcaag atcgattgaa gcatcacgca tcagtcacca ccaccgcagg
    16261 aaccatttgc atgcccaact ttcggcatcc aactacgatt gtatcgtaca ttggccagaa
    16321 acaacaggat gaaccaggga gaagaaactt gggaatccgg gtgtatgact gacccgtggg
    16381 tgagctagaa ggaaaaagat gactcagcgg tggatcgctt ggctccacca aagagtcatc
    16441 ctaccaaagc tccatatact tcgttcagtc ttttagggca ccatcgagca gaatctggat
    16501 gagttgatcg aaggaaatcc ctcgcgcccg ggcggcgtca ggcagaaggc tcacctcagt
    16561 gaggcctggg agcgtgttca cctcgagaac ccatacagtg ccattcggtt cgactatcat
    16621 gtcgactctc gagtaaccgc gacacttcaa ggcagcatgg gcagccaaag cgagcctctg
    16681 gcactctttc tgtgcggcat ttccaattcg agcagggatt acgtgctcag acatcccagg
    16741 agtatacttc gattcccaat cgtagaactc gttctttggc actatctcaa gcaccggcag
    16801 cgcctctgta tcatggttac ctaggacgcc tacggtaact tccgtaccct tgatgaactt
    16861 ttcgacgact gcttcttcat catagttgaa ggcgtacgat aatgcatctt ggagagcgcc
    16921 gtcatcctta actatgctta ttccgacagt cgacccctgc gaagatggct tcactacaca
    16981 cggaatgccg atgctcaagg cgcgctccaa agccgattgg aacggggaat ggcaatccac
    17041 cactacatag gggggagtag ggattcctga cacttcaaag agcgccttgg agattgtctt
    17101 gtccatggcg attgcgctgg agagcaccga tgagccggtg tatgggatgc ccatcaactc
    17161 gagggcccct tgtatacaac cgtcttcgcc gtatttccca tgcaacgcga tgaaagccac
    17221 atcggggcgg agctcggcca gcacaccgat ggcgttgtcc gccgggtcga ctgatatgac
    17281 ctcatggcct agcctgcgca aggcggcctc tacctgtgcg cctgatttga gtgagatctc
    17341 tctctcactc gaagatcctc ccatgaggac agctactttc atctgggttt gatcatccaa
    17401 tgacgtcacc gccgtttcta gctgaagtag accgacactg cacactatca cttgggccag
    17461 acacgaaaaa tctcccgcaa tgtgcaggtg gtcgtcttga tcactatgag aaggtatgcg
    17521 attccgcaga caagcagaaa tcctcgggcg attgcagcat gacgccactg ggcatcgctc
    17581 atatcggcgc tccgcttgaa ccagagcacc acgacgagaa taaccacggc aataacggat
    17641 ccgatcatga gcaaagccgc catagcttca tcaccgatgc atactatgcc gccctacaga
    17701 cgaatgtccc actccttaag catatccgct gtacgccgac aagttgcatc gaaccttaag
    17761 ggagtgattg aaacagctcc ggttctgatg gcttccgtat ccagtccgtc atcggatgga
    17821 ggggtatccg ggtccccgga tagccaatag tatggtcgtc cacgaggatc taccctacgc
    17881 tcgaaaatgt tattataccg ttgaaccccc agcctagtga cggccacctt gcctgagttg
    17941 aagccatccg gaacattcac gttgagcacc accccttctg gcaggcccct agtggccact
    18001 tgagaggcga gcttcgccgc tacgcgagcc gctggctcat agtctgatgg gtcatatgat
    18061 cctgtggaaa ccgcaatcgc cggtatgccg gcaaacgcgc cttcggctgc agcagctact
    18121 gtgccagagt atatgacgtc ggttcccaag ttaggcccgc ggttgatgcc tgagaggact
    18181 atgtcgggac gttccggcaa gagctcttct agggccagct tgacacagtc ggacggcgtg
    18241 ccgctgaccg accacgcctc agcctcgata cccctaaacg cagtcttctc cgcccggagc
    18301 ggaagcccca ttgtcagggc atgacttatc gcgctcctct ctcggtctgg cgcgcagatc
    18361 cagagcttat ctgcgatttc agcgatggct ctggcaaggg ccagcaatcc aggcgcccat
    18421 atgccatcat cgttcgagat caatgctttg agaatgggaa agacctccta ccgtgaccca
    18481 agactagaat ctctcgatct cggccttaag tatcccggaa aggctgagaa catgctgaag
    18541 caagtctgcc tgggccaact ggggatccct gccaaccatg ttggacgaga ttgtgacgtc
    18601 gatcgataca atagaattct ccctgtcaac gtttatctcg gtttcgacta tgttcattcc
    18661 gtattttcca attatagtgg caatttcgcc aagtagcccc ggccggtcaa tggcgttcac
    18721 cttgagcacg gcttctccat gtggaaaata ttttctttcg accctgctca taatcaccaa
    18781 aatgaactgg accagcactg ttgcgcctat tgccggccac accattcccg ctccaatggc
    18841 aaggcctatt cctgctgtgg tccaaaggct tgctgcagtt gtaagccctc tgatggagag
    18901 gccttctctc atgatcgtgc ccgctcccaa gaaaccaatg ccgctgacaa cttgcgccgc
    18961 aactctggca ggatcgtgca gaccggaacc ggcagggcca aaaccatgta tgctgactat
    19021 ggtgataagg gccgacccaa ggcataccaa ggagtgcgtg cgcaagccgg cggacttgcc
    19081 ttgcctctcc ctctcaagcc ctattactga gcctatcaat gttgcgaggc tcatcagccc
    19141 taactgctgc cagagcaaag ctctatccat attcccccct ccaatcgctc tagcttacca
    19201 ctctagcgtg ggattctgct acgatgccca ttacttgctc ggcggttgcg ctcactagtt
    19261 cgcgcgcctt gtccaacgcc tctatgacgg gagtaaccgc ttcgtcgcca gctatctcct
    19321 tcaatgcttg cagggcggga atgcgcaagt cctcgttttc tatgcatatg gcgactagag
    19381 gttctactgc cctgatatca ccaattattc gaagcgcttg tatcgctgcg attcggacat
    19441 cgcgcgagtc gtcctcgagg cagtctgcca agacgtcgaa tgaagctgga tcacctatct
    19501 tgcgcaatgc ctccactgct tcatacctca cgccgcggtc tgcgtcgcgc gccaacgcaa
    19561 tcaggtgcgg cactgcccgg tcttcgccca tatcgcccac gagtttagct gcgcgttttc
    19621 ttacggtgat gtctttagaa catagttttg cttcgatcag gggccacagt ccatcgcgat
    19681 ccacagactt cagagcgttg cacgcgctgt agtagacgta tacgctctcg tcgcgtaatg
    19741 cgcggactag cggcaacaac gccgcattgc cgccgcacga agacagcccc tcaaccgccc
    19801 agaccctaac tccctcgtca gggtctgaaa gcgaatctgc aagcagaggg atgcatcttg
    19861 agtcctccaa cttggaaagc ccgtaggctg cggctcgccg cgtagcatca tccccggttg
    19921 ccaagaggtc ggccaagagt cccaacaatc gcgtcctgat agaagctatc tgatcagcaa
    19981 cgttcggttt acaacagata tccgcggttg ttaaatcctg ctccagggtt ttaagttcct
    20041 caactagagc ctctattcgc ttggaatcaa tcgatgctgt agacaagaaa cgcacccccg
    20101 gcacatagtc tcaaggggca aaatcataag caataagtca tttggctctg agagcgaatt
    20161 tgttctcagt gcccgaaagc agtcgctcaa tattcgatct gtgccgcaca acggccaaaa
    20221 cggccagaat tgcgcagact ataacatacg ctgaaggata tcccatgatg taagcggcta
    20281 tgggggttgc tgccactcca accatcgatc ccaaggacgc atacctgaag gcgcacacca
    20341 gcccgagcca gactgcggca aggatcaggc caaccggcgg cattatggca aagatgaatc
    20401 caaaagtcgt ggctatgcct ttgccgcccc tgaatcccag gaatatcgac cagttgtggc
    20461 ctgccacaac ggccaaccct gaagccgcga tcacccaatc attagccccc agggcacgtc
    20521 ctagcaagac aaccgccagg ccctttgagg cgtcgagcag gaacgtcacg aacccaaaag
    20581 gaaacccgag ggtccgaaga acattcgtcg cgcctatatt tccacttcca tgctttctga
    20641 catctactcc gaccaaagaa gtccctagca gcaagccaaa agggatagag ccgataacat
    20701 aagagatgga caagaggatt actggcgaca atgtcactgg gcttcaccct tccgatctag
    20761 tctctcgctc tgaactgcac tattatgggt gttccggaaa actcgaacgc ctcgcgaatg
    20821 gcattctcta ggtaacgctt ataggaaaaa tgtatcaact ccgggtagtt ggtccaaacc
    20881 acgaaaagcg gaggacgcga cgatacttgg ctcgcgtaga atatgcgaac cgccttcccc
    20941 ttgtcgctcg ggggagggtt tcgcaggact gcgtcttgca ctactctgtt caccatcgat
    21001 gtctgaatcc tgcgtcggtg gtgcattgcc cctttcctca ctgcgtctag gagtttggtt
    21061 attcccctcc ccgtaagggc cgagacgaaa aggacatcgg catatggcat aaaattcatc
    21121 atggttctga ctttgcgttc ataatcccgc gcgaaagcct tatcccgatc aaccatatcc
    21181 catttgttga ccgcaataac ccctgctttt cctgcttcat gggcgtaccc ggcgatcttc
    21241 gtgtcctgca caaccggatc agaagtacca tctatcactg ttaccgccac atcgcatctg
    21301 tccaccgcgg caaaagccct gagaacgctg tacttctcca ccccgggatc gaccttgcct
    21361 cttcggcgga tcccggcagt atcaataata acatatttag tcccatcgta atcaaagact
    21421 gtatcgagag cgtcccgcgt ggtgccgggt gtatccgaaa cgatggaacg ctcttcaccg
    21481 agaatcctgt ttgccagcga agacttcccc acattaggcc tgccgaccag cgccacccgg
    21541 attggatcat catctttttc agcaggctca tctgtttgag gaggcaatgc ctgtacaact
    21601 gcgtcgagta agtttcctat gcctattcca tgaaccgccg agacgggcat cggatcccct
    21661 agcccgagag cgtagaattc gtagtccatg tgggccggat gatccacttt attcgcggcg
    21721 agaatcacag gttttccggc tatgcgcagc agctgggcca cgtccttatc gtcagatgtg
    21781 atgccagaac gggcatctac aacgaaaatg atgacatcag ccgctttcat ggcgtgctcg
    21841 gcatgttgtc ttgtctgcgt cgagatgtcg tctgctggat cggcctcaat cccacctgta
    21901 tcgacaatgg caaaggatct acccgaccac tccgccgtgg agtacaccct gtcacgggta
    21961 atgccgggcg agtcgtccac gatcgatgat ctgctcctta ttatgcgatt aaagagcgca
    22021 gacttgccga cgttaggccg tccaacaatg gctacgagcg gtttagccac atcattcacc
    22081 tcgcattcgg tgggtgcaga gcaagtattg actcgacgag ccctcgcgcg gtggtagggg
    22141 ctaccaccac cggaacgccc acagaagaag ctatgccctc cactgtgaca tcgtcaagca
    22201 gagacctttc gccgcttttc agcatcacgt cggggatcac gacgaaatcg ccaagacgac
    22261 cccgggatgc ttcttcctgc aaagaccgta tgatgtctgc gccaaccacc agtccggaaa
    22321 cggtaatgga ttctccgaag aacttattct ccacaggcac caaccggacc tttaccccgt
    22381 gaatcccgtt caaagagtcg cacgcgtcgc gtattactcg cgcgcctaga acgcccgtta
    22441 ccacagttgc gcagatcctt cccgcgactg agcatggaag ttctagagca atctgagcaa
    22501 attcatccag aaataggcgc gcaagtccta tgccgttttc aatctgagga tactcgtcat
    22561 atgcgtcagc tataggaaaa ggcagagacg caagacagta aaactcatct gctgcgtaaa
    22621 cgaagtggtt tccaaaaagc ttcatgcatt cgttctgcgc cgaatcgact atctccagag
    22681 tctgacgcgc gataccagct gatacaggct caagttcggg cagaccctgg cgatgggagg
    22741 taaggcccac aggaactacg cccaccgaca cacatgatgg ccacaggctc tgcagatccc
    22801 tgatgctcgc ggcaagcaca gtgccatcat ttatgcccgg aaccatcact atctggcagt
    22861 ggaaatcaat ccgtgcctct gccagccgct tcagcgtagg catgatgggg aaggcatgtg
    22921 tcctcccaag caacctgcac ctggtcacat cgtccgtgct atgcacggat atgtacagtg
    22981 gagaaagccg catgtccatt atgcgctgaa gatcgctctc cctcaagtta gttagggtga
    23041 tgaagttccc ctgaagaaaa gagaggcgat aatcgtcgtc cttaaagtat agagtatccc
    23101 gcatgcccct gggcaattgg tctacaaagc agaaaacgca tttgttcgcg cagtgcttct
    23161 ccccgtcgaa cacgagctcg tcgaatccaa gcccaagacg gggatcgtaa tcattgttaa
    23221 agactgctgt cagcattgac ccgtcaggct tttcgatctc gagcctcgtc gtgaaggagc
    23281 taacatgata ctcatagtct attatgtctc ggattggatg tccatttaca gacaccaata
    23341 cgtctccggg gcaaatgccc gccgcatcgg cacggctgcc ctcatcaact tgtgtaattc
    23401 tagcccgagg ctgcatgata ggcgtcatag acccgtgcct ccaagaaaat caaaagggag
    23461 aggaaagtcg cactctcctc tcctcgtaga tgttatccgt ctgccgctct tgctagagct
    23521 agcgaaggtt attccttgat gccgaatggg gtcgttgtcc caagatcctt gtacttggaa
    23581 tcagttatcg taatatcgct atcaagcttc agctcgctca ctagctgctg agcggtctta
    23641 gactgctgca tcttaaggct tctttcgata tcgtccctaa cttcgtcgaa agaggcttcg
    23701 cgagcggctt ttctatccat gacttttatt atgtgatagc cgaactctgt cttgactggt
    23761 ccgctgatct cgcccggcga gagagcaaat gcagttttct caaattcttc tgtcattcgt
    23821 ccgcggggga accaacccac atcgccccca ttgactgcag ttgcaacatc gagcgacttt
    23881 tccttggcaa gctcggcaaa gtctgcgccc tcagaaagct cctgcttgac ctctttggcc
    23941 tcttcttcag ttgcgactag tatatggctg gccagaactt gttcgggctc gtcataatct
    24001 gacttgttct cttcaaagaa ggccatcaga gcatcttcct ctattgtgat atccttcgtg
    24061 gatatcttgt atacaaggag gttcatctcg ataatatctt tgatttgctc atcggtccag
    24121 ccatactgag cgatcagcat gtcgtaagaa ggcccgatct gttccttgag tagagctact
    24181 tctttatcta cctcttcagg cgttacttcg acccctgcct tcttggccgc ctggtgaagc
    24241 agagtctcat ttatcagttc tgctagggta tcctccccgt attgctcctc aagcctcgcg
    24301 atgaaatcgc gccgcgatat cctctcgccg ttaactatgg ccacatgtgc cgagttggtg
    24361 taaactatcc atattatccc ggccacgacg attgcagcca caattatggc caaccgaaca
    24421 agaccggcct tgcgcttgtc catgcttgcc aacccccttc tctcaactac gatcctgctt
    24481 acgtcctgct actggcttaa acgaactatg ccaggcacga gttccacata aaaatgctac
    24541 ggctagtcta gcatagtgac gcaggactgt caacgaaacg ccagcataca ggctgatatg
    24601 atggcagcaa tcctacctgt cgtctccgtc agtcgaaccc ttaagcttgc tgggatcgta
    24661 gttgattcgg tcggcgatgg tgacgacatc gctctcttta ctttccttca tgtacctgcg
    24721 gtattcattg cgctccctat cttccatcgc ttgccgtatt gacaggctga tgcgatgctc
    24781 ctgctccctg aggcctatga ccttgactgt cacagtttcg ccaactgaca cgacctcatc
    24841 cggccttcca acgcgtctgt cggcgagctg cgagatgtga acgagtccct caatcccgtc
    24901 ttcaagccgg acgaaggcgc caaaatcgac gatcttcgtc acggttccag agactacaga
    24961 acccacaggg aaccgctggc caatgttttc ccacgggtca ggaaggagct gcttgtatcc
    25021 gagggatatc cgttcgcgct cgcgatctac attgagcacc aggaccttga tggactgtcc
    25081 ctcagcgagc acttcgccag gatgctttat cctggaccaa gagatatcgg agatgtgaag
    25141 caggccttca actccgtctc caatatccac gaatgctccg aacgacacga gtcgggttac
    25201 tgttccatca agcacctgac cccgctcgag cgtttggaat gcgcgctcct tagcaagtct
    25261 ttcttcctcc tcaagcactg cacggtgaga aagcaccaca ttggaacgga gcctttcaac
    25321 ttcgcggacc ttcagcctca acgtctgtcc aacatagaca ttcaaatcct ctacgaacct
    25381 gcgccctacc tgcgacgcgg gaacgaaacc tcggactccg atgtcgacaa ctagcccgcc
    25441 cttaacagct tctgtaacct tgccttcaat aatctccccg gtatccatgg cgttctcaag
    25501 cgtttgccac ttgaggaggg tgtcggctcg cgtcttcgag gcatacatca tgtcggaact
    25561 cggacgttga gaatcgtcga gcaccacgta gatttcgtca tccacagaga ccacctcaga
    25621 tggatgagag atctgctcaa tgctcaaatg ctttttgaag atgaccgcat ctttgcccgg
    25681 ttcgacttct acgaccactc tgtcgtcata gacctctttt actcggaccg ggataacgtc
    25741 aggatctgac gggagctcgt gagcaggagg cgtaaatctc tctgtggcag gcttctcctc
    25801 ttcagcttca acctgctcgc catctccagc ttcctcctgg tctgaatcgg catcaaccgt
    25861 ctctgccccg ccagtgtccg gcggagctga tccttccgca gcttcttctc cgccgtcgtc
    25921 agaggattcg tccgtcccga cctctgaagg gctagtctcg gtcttctcgc attcctcagt
    25981 tgcgctggtg ctttctgacg gttggaccgc gactcgctcc gcgctctcct cgggcacagt
    26041 ttctccttgc tcgactgcgg tctcttcgcg ctcctttgct agctcagtcg agctagcctc
    26101 gcaagtctgt tcgtcttggc actcaagaca tttggcttct tccgagttcc cctcgataat
    26161 ttcgtgcata gcgtccttga cctcctcaat tacccaacta ggcgtggatg ctccggccgt
    26221 cacgcctatt gtgcgggcgc tcagtatcgc ttcgcgatcg agcccgcacg cgttttccac
    26281 cttaagcacc cgagcgccgg attcgcggca tacttccgcg agacgcatgg tattggcact
    26341 cgctgcaccc ccgatcacaa ccattaggtc gacttctccg gcaagagtcc tggcggcttc
    26401 ctgcctttct gaagtggccg gacatatggt attcacggca tcgactaccg ctccccggtt
    26461 gcgaagaacg gagacaactg cctcgaacag ggattgtttg tgcgtggtct gtcctatcac
    26521 cgtgataggg cgcgccggca gcgtcaggga ttcggcttcg tttggatccg aaacgaccac
    26581 cacatcgccg gctgcgaatg cggcgactcc acgaatctcc ggatggtgct tatctccgac
    26641 gattaccacg taccggcctt cactatgtgc cctggccact tcccgctggg atcgaagcac
    26701 atgcgggcat gtggcgtcca cgagcgttaa ccctctctcc cgggcagcct gaatagtctc
    26761 gggagggagg ccatgcgacg gaattatgag cacacgacct gaggcagact cgatggagtc
    26821 aacttgttcg acgccacatt taaggagact cgacaccaca tcggggttat ggaccaaagg
    26881 ccccagggtt accgcgccgc ctgagccgga gcgcagtgct tcttgccctg cctcgcacgc
    26941 gatgcggaca gctcgttcaa ccccgcggca aaatccggcg tgtttcgcct ctcttatgat
    27001 gggcgcacaa gtcacttttt tatcgcctgc tcccagtctg ctaggaatcg ctctatccct
    27061 gcatccgtaa gggggtgctt cgccatctgc atcaatacct tgtaagggac agtagcgata
    27121 tcagatccga ccaacgcagc ttctatgaca tgcattggat ggcggatgct ggcggctatg
    27181 atctcggttg caatgccgtg aatatcgaaa atagaagccg tttccgcgac cacattcatc
    27241 ccatcggagc taatgtcgtc gagacgtccc acaaacgggc tcacgtatgt ggcgccagct
    27301 ctggcagcca acaatgcttg gctcgatgag aatatgagtg taacgttggt gttgattccc
    27361 ctggcagcaa gaacctttac ggctttgagc ccctcagatg tcatcggtat cttgatcact
    27421 atattggggg accaggtcga gatctcggct gcttcgcgga tcataccatc cgcgtcctgg
    27481 ctgatcactt cggcgctcac tggcccgtct acgatcctac agatttcaga gacaacttcc
    27541 ttgaagtcgc ggccttcttt tgcaacaagc gacgggttgg tggttacacc gcatacaaca
    27601 cccatttcgg caacttcgcg tatctcgtca atatttgcgc tgtcgataaa aagcttcacc
    27661 ttcgcacact cccatattct cacgcaatca taatcagttc accgatgcgt ttcattattt
    27721 cttcggcgag cgcgtccgtc tcttccctga cagttgctcc cgctcgatcg acttttggcc
    27781 atatgagctc accaaagacc accctgacca tgggttcttt gaacctcttc aaccctttgc
    27841 gatgccccgc aaaggttcca acaaccgctg caggaagcac gggtgtccct gtctgtatgg
    27901 caaaacgcgc agctccagga tacgccttct gcagttcccc ggtttcgctg cgcgtgcctt
    27961 caggaaacat gcctagagct cctccagcct taagtatatc cattgattgg cgatatgctt
    28021 cacggtcaac cctaccgcgc ctcacaggaa aagcataaag ccacctcaaa acggcagcca
    28081 agatgggatt ctcaaaaagc tcggcctttg ccatgaagcg tatccggcgg tcgcaagcac
    28141 agcccagagc tatcgggtcc aggatgcttg cgtggttcgc tactatgatg accggaccag
    28201 ttgcgggcac gttttctttt cccgtcacct taaatccgaa ggcgatggat aagtatagtg
    28261 tcatcacgaa ttttgcgaaa gagtaaaaca agaagcaggc ctcctaagag cagctaacat
    28321 cgtgtcgacc acctcatccg gagacagtgt agtgcagtcg atgacaactg cgtcaggcgc
    28381 cggtctgagc ggagagatct gtcttgtact atcaatggtg tcgcgcttct cgatctcctc
    28441 gactagatcc gctaggtcaa catggcgtcc ctgtctggcg tagtcaagct gtcgccgtcg
    28501 agcccgctcc tcagctgatg ccgtcaggta aaacttatgc gccgattctg caagcacaac
    28561 tgtacctatg tcacgaccgt ccattactac gtccgcgtcg tatgcgattg ctctttgggc
    28621 ttcaaccatg tgcttcctaa cctgtgagta cactgacacc ggtgagacaa ctgagtcaac
    28681 ttctgcgctg cgtatctctt cggtgacgtc ctcattgttg agaatagtcc gctgtccgcc
    28741 gcactcgctg cgctcaaccc ttatgcaaac agattttgcg acatccacaa ccgccgcctc
    28801 atcgtaggga tctagccctc gacgaatgac ttccagggcc aaagcgcggt agaggtcacc
    28861 agtgctcaca tacctatatc caagtgcatg ggcgagcatc cgcgcaaccg tgcttttccc
    28921 ggctccagcc gggccgtcta tggcgattga atgttccagc gacatacatc aacgctctcc
    28981 aatcagtgta caatttaaat ttattctacg ctagtacgct tattcctctg cgacaacgaa
    29041 aaatccttct gcgttcgtat aatctatatt attataacat tacatatttg agtgcgtccg
    29101 ggaccaatag agcacttatc tcccttctgt ggctttgtca cgtttggagt aacatcttcc
    29161 acctctattt ctagttgttc cacgtgcgcc ccgccagccg tcgagtcgat ccgatcttca
    29221 tcggcattgt ttacctccac ctccagcaag tctcccctgt gaattactac tgccgcgctt
    29281 ccatacttga aatctgcaac gggttttccg tttaccaaga ctgtgagggt gggctctgcg
    29341 cattccagac cgttgtctag agcggatagc tttaagagta ttccgccggc ctcaacggca
    29401 gcaccagccc gcacgtcggg tttgccgcta tcgccggact ttccaggcga aggtgtccat
    29461 gaccatgagg gatgcccttc gatgcgctct atcatcagca cactccttgg aattactcca
    29521 caagaaagca ggacttgcac caaaatgcag gctgaaaggg caaggcctgt aagggcggag
    29581 aatacaatgg tcgctacatc ccaattttgc aaaggtctag ccactccttc ttgacattgc
    29641 ggcgctcatt cctgccagac gtcctgtgga gaacgcggct tggagattgt agcctccggt
    29701 aggggcgtca atatcgagaa cctccccaca aaagtatagt cccggcaaaa gtttcgactc
    29761 cagcgtgcgt gggtccactt cccggagcgc tattcctccc gaggtaacta tggcttcctc
    29821 aatagggcgg gttttcagca cggttaaagg cattcgcttt agcgtctcga ccaatgccga
    29881 tctttgggca gctgtaatct ggtttgcagg gatagtctcg tctacctgtg caaggcttat
    29941 tacaaccgga ataatcgagt gtggaagaag catccccaag gagtttttga gctgtttttt
    30001 ggatgcggcc acgagatctc gctgtattcg gcgatggagg gtggcctcat ctaaggcggg
    30061 ttttagatct atggagagcg tgaaacgtcc actctcgatc gcttccgact ccctgagaga
    30121 tctgcatact gcgcgactca ggctgaggac gattggtccg ctcacaccga agtgggtaaa
    30181 gagcatttcc ccaaattccc tacctatcct gcgtcctctg tcgtaaaggc ttagctccac
    30241 gttgcgcaga ctgaggcctg cgactgatcc tggccaatct tctactgttt ccatgggaac
    30301 gagtgcaggc aaaggctgca cgatagtgtg ccctgcctcg gcggcaatgc ggtagccatc
    30361 ccctgtgctt ccggtgcccg gatacgagat tccgcctgtt gcgattatca cggcgtctgc
    30421 ccaagaattg ttcccgccgg agtcgattac gcccttgact ttgccgtcgg cgactgcgag
    30481 cccggttatt tgcctgccaa aagcgaaacg agccccttga ttcgtagcat ggcggtacag
    30541 cgcgttttgc acgtcagctg cactgtcgct cacgggaaag accctcttgc cccgttccac
    30601 cttgcactcc acgccaaggc ttaagagcaa cgcttgcagt tcctcgggcc caaagttgga
    30661 catggccgaa tagaggaatc gcccgcccgg gccgaaagcc tcgactatgt ccgcgactgg
    30721 cgaatcgttt gtaatgttgc atctgccttt acccgatatc aaaagcttct ttccaggaat
    30781 cctgtttcgt tcatatacaa cgacatcggc gccggctccg gccgcggcgg ctgccgccat
    30841 gagaccggat gctccggcgc cgataattgc tacatccaac ttgcttcctc cacttctgcc
    30901 gttaatccga tttcatttta tctttcccca atgggcaatt cccgccatga accggggacg
    30961 gatctaggcg gcccatccac ctcgccaagg ttgatgcttt tcagcttgtc agcgtaaacg
    31021 cctgtgggaa aggctaacct tccagatccg ttatacgtaa ttgagcttcc cacgatcgct
    31081 ccgctgaatt ccccttttcc gttaaacacg acatccgctc taggcgcata tatcacagcc
    31141 gccatgttcg gaaacccatt catagtaacg ttagggggaa agtcacgcgg gttctttggc
    31201 ccagtgcagt agataacgaa ctttgtagga tctaaaggag gtgcacctcc cctgctaccg
    31261 ttccaggtga aattgccgtc cacatatagc tttagcgcat ggtccccgat gatcttcag
//
LOCUS       DAOSYL010000025        31026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1201_length_31026_cov_8.460302, whole genome shotgun sequence.
ACCESSION   DAOSYL010000025 DAOSYL010000000
VERSION     DAOSYL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 31026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31026
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1201_length_31026_cov_8.460302"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..451
                     /gene="rpmH"
                     /locus_tag="PLU15_05745"
     CDS             317..451
                     /gene="rpmH"
                     /locus_tag="PLU15_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOO30459.1"
                     /translation="MKRTYQPKVRRRNRVHGYRKRMSTPAGRNVMRRRRAKGRKRLTV
                     "
     gene            563..940
                     /gene="rnpA"
                     /locus_tag="PLU15_05750"
     CDS             563..940
                     /gene="rnpA"
                     /locus_tag="PLU15_05750"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOO30460.1"
                     /translation="MPTVSLRRTSDFQRAFREGKSAAGQYVVVYAVPNGLPEVRLGYP
                     VGKKLGGAVKRNRIKRLLREAARLAPNMPGMGYDMVLVPRRKIADPNVRCHNVIGDLE
                     KVLDRLSLETELGEPRSCGGSDV"
     gene            959..1177
                     /gene="yidD"
                     /locus_tag="PLU15_05755"
     CDS             959..1177
                     /gene="yidD"
                     /locus_tag="PLU15_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOO30461.1"
                     /translation="MWAVRIELGLIRFYRTCISPLKRYPCCRYVPTCSQYAEEAVKKY
                     GVIRGTWLAMKRLARCHPFSAGGYDPVK"
     gene            1245..1877
                     /locus_tag="PLU15_05760"
     CDS             1245..1877
                     /locus_tag="PLU15_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOO30462.1"
                     /translation="MGITGVMQSILRFFAEGTGNLAWAIIIVTTLIRAALHPLQVKQV
                     RLMEQMKEITPKVQELEKKYKGQPEEYQRRVAELYRDNNVNPAVGCLSMLIPLPFFVI
                     FYRVLQDTAFIGGLPEAAKGFLWIKDFALPDPWALPILSAVTTWLSSRQTATDPSQKS
                     MAIIMPIFMGWIAMRMPAGAALYWVTNNLISVVQQWLIQRQLAAAKKGVS"
     gene            1878..2648
                     /gene="jag"
                     /locus_tag="PLU15_05765"
     CDS             1878..2648
                     /gene="jag"
                     /locus_tag="PLU15_05765"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOO30463.1"
                     /translation="MEPVEAVGKTVEEAVESGLSTLGLARSQVEIEVIDEGSKGLFGL
                     VGRRHARVVVRPLANIESKGEAYEGAMGAVESPVRAEEDLGGIDEDDDEWRGGSSFDE
                     DDAKSSGYVFLEELISLMGVDVDITETRSEDITLLEVEGRSLGMLIGRRGETLDAIQY
                     LVNLAANREARASGSSERERYVVDIGGYRKRREEVLENKALELAERVVQEQRDEVMEP
                     MSALERRVVHMALRDYDGVVTHSEGREPYRRIVISPKE"
     gene            2827..4302
                     /gene="mnmE"
                     /locus_tag="PLU15_05770"
     CDS             2827..4302
                     /gene="mnmE"
                     /locus_tag="PLU15_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOO30464.1"
                     /translation="MFREDTIAAIATPPGIGGIAVIRISGPEAKDVAQRVFVPARPGA
                     ASDGAAGLYPIRGWLCKYGHVHDASGELIDEAIMTWMPGPTSYTREDVAEISCHGGTA
                     VARAVLEATLKAGARIAQPGEFTRRAFLNGRISLEQAEAVLSMVNARTERARKAASSF
                     LAGSLGVKVREAEESIISLMTVIEASLDFPEEDIPDVAVQEIADESSRIAAMLEEACQ
                     EASMGRLLWEGASVAIVGRPNVGKSRLLNALLKEERAIVSELPGTTRDVVSEVANIRG
                     IPIRLIDTAGMRDSVDPVERIGVERAKIALADADVALAVVDGSEPLDMADAEIVSQTP
                     ESHTIVVVNKCDLANVLGDEDARRIAAGRPVLRVSALTGEGLDNLEEAIEALVLGDRG
                     AGIAEIGALAGQGLHGGQAADQRDGLETESEMLGMRREDCLRRAAQSLRDVACGVEEN
                     MPPDMLSIDLRSALDALGELTGETTREDIVDRIFAQFCVGK"
     gene            4373..6133
                     /gene="mnmG"
                     /locus_tag="PLU15_05775"
     CDS             4373..6133
                     /gene="mnmG"
                     /locus_tag="PLU15_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOO30465.1"
                     /translation="MDYDVIVIGLGHAGCEAALACARLGCSTLGLTVSMENIAQMSCN
                     PSLGGPAKAHLIREIDALGGEMGLAADETFIQLRMLNTSKGPAVRGLRAQIDKAEYQA
                     RMRRTLQRQPGLVLTQATVYDIIVRNGRVVGVSTEIGEISSRAVILATGTHLGGRVHM
                     GEMNYESGPDRQPAAVELGENLRRRGFAMYRFKTGTPARIDGRTVDYDATQIQLGEEL
                     PYGFSYLRRPYPREQIPCWQTWTNERTHEVIRENLDRAPMYTGAITGTGPRYCPAIET
                     KIVRFPDRPRHQVYVEPQGRGNEEMYLSGVSTSLPVDVQEKMIRTIRGLENVHIMKYG
                     YAIEYDCIDPTQLAPTLESLKIAGLYFAGQINGTSGYEEAAAQGLVAGINAALSVLRP
                     DERLLLDRSEAYIGVLIDDLVSRGTDEPYRLMTARAEYRLLLRQDNADLRLTPRGREI
                     GLVDEDRWERFAARRNAIELIRSGEELPEALRDYAEEATEQVEIESKFSGYIAKQRRQ
                     VEEFKKLEKWKIPEDIDYTKLTRLSREARERLGVVRPFSVGQASRVPGISPADLLALT
                     VHLREMTLQGRKEEAARDNG"
     gene            6126..6854
                     /gene="rsmG"
                     /locus_tag="PLU15_05780"
     CDS             6126..6854
                     /gene="rsmG"
                     /locus_tag="PLU15_05780"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOO30466.1"
                     /translation="MDEFVYYLKQGACELGVALSEPQIEMFCAYKTMLVHANRFVNLT
                     SITDDQGIAVKHFVDSLSCLLVAGTENFNSFVDIGTGAGFPGLVLKIMNPGARALLVD
                     SVRKKVDFVREVADSLGLDGVEALHGRAEDFGQDGEYREQFDLAVARGVANMGVLAEY
                     CLPFVRPGGLFVAMKGPRVVDEMDAGGKAIEILGGSQAILRHLALPYDAGERVLVAVR
                     KERNTPAKYPRKAGAPARKPLGDA"
     gene            6951..7718
                     /locus_tag="PLU15_05785"
     CDS             6951..7718
                     /locus_tag="PLU15_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO30467.1"
                     /translation="MGRVIAVANQKGGTAKTTTAVNLGAYLAMAGRRILLVDMDPQGN
                     ASSGLGVDKRAVESCIYNVLINDIEIEKAILNTGVQGLDIVPATLNLAGAEVEMVSLM
                     SREHRLRKALEPVRVIYDYCLVDCPPSLGLLTVNALTASDSVLVPIQCEYYALEGLGQ
                     LMRTIDLVKRHLNPELEIEGIVMTMYDARTNLSQQVVEDVKNNSGQHVFEAVIPRNVR
                     LSEAPSYGVPISMYDDRSKGAEAYRSLANEIMQREGA"
     gene            7721..8587
                     /locus_tag="PLU15_05790"
     CDS             7721..8587
                     /locus_tag="PLU15_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOO30468.1"
                     /translation="MATKRGLGKGLNAIIGEAMLNEESHELRGIEIPLDQLTANPFQP
                     RAEFDPDKLEELAESIRAHGVLQPIVVRRAGENYQIIAGERRWRAAQMAGLQTIPAVV
                     KELDDPGMIQVALIENLQREDLNPIEEASAYRKLMDEFSMTQEQLSGTLGKSRSTIAN
                     AVRLLNLPDEIQEHVSRGRLTGGHARCLLALDDAKLQLEVAKEVIDKGLTVRQTEELV
                     RRLVKNVSRETIEQAPQVPDPDVVAIIRHLSERLGTKVKITGSSGKGKVEIEYYSEDD
                     LGRILDIILGIA"
     gene            8614..9180
                     /locus_tag="PLU15_05795"
     CDS             8614..9180
                     /locus_tag="PLU15_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HOO30469.1"
                     /translation="MDWRRDRGLVWPGLDMEQSAVIGLICAQQQLVPMILSIILLISV
                     VVFINATNRIRRLEKAYRSLLGTESEEKDIEQIIMEYIRKTDMLQDQLDETRKELERA
                     QDAALAYIQKVGIVRYDAFDDTGGEQSFSMALLDANANGVVVSGLSGREETRVYAKPV
                     KGGRSPHTLSDEEIQAIARAIESSKEKL"
     gene            9347..10411
                     /locus_tag="PLU15_05800"
     CDS             9347..10411
                     /locus_tag="PLU15_05800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO30470.1"
                     /translation="MQQRFIRRVPARKRQGSLTVIAIPSSGEPPRSVGIPTQMLAICA
                     AALIGVIGIFVISYLNMSRGIATLNEAKMDSDNAIEQLLSENQTLKLEKQERLAKMQE
                     LASKTQMLEATVSRIADEKAEIWAIVKGSDAAQKNASSSNAVSSRGGASRGGAPGAER
                     ESPEAVLDAVANAPAVDVGADAFDGEQYLVETMAEELEMLLVATSAMQKDLSDLRVSA
                     ETYRSKLDHTPSGWPVNGRITSAYGNRRHPILRITRFHEGVDIAATHGTPVVAAADGV
                     VVLSGWSGGYGNAVEISHGFNCSTFYAHNSRNVVKVGQKVKKGQIIAYVGSTGLSTGP
                     HCHYEVRIGGRHVDPAPYMK"
     gene            10447..10866
                     /locus_tag="PLU15_05805"
     CDS             10447..10866
                     /locus_tag="PLU15_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOO30471.1"
                     /translation="MFRRRPEKAPGAARIDTYIGKGTTVEGSIEASGSVRIDGFVKGT
                     LRVDGDLIIGADGVVVASVTAANVTVAGELRGEVNSPGRLDIAPTGQMLGDVIVGSLN
                     VDDGALFLGQCNMKDEVRSQPKVLAAPDDSGNSDSQQ"
     gene            10972..11619
                     /locus_tag="PLU15_05810"
     CDS             10972..11619
                     /locus_tag="PLU15_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOO30472.1"
                     /translation="MQFRDRAEAGALLGRRLQQETWDDPIVLALPRGGVPVAECVADA
                     IGGELGVVVTRKICAPHNRELAIGAVDADGRMFIDSRIVQALAVDDSYIRAETSRQLQ
                     EIERRMAEYCENGDVPNRKARDVVLVDDGIATGYTILAAARYVRRHGPARTVIATPVC
                     PPDVFSKLQEEADMVVSLLLPAHFIAVGQFYEDFSQVSDADVRNILTRVYTKGSR"
     gene            complement(11620..12243)
                     /gene="yyaC"
                     /locus_tag="PLU15_05815"
     CDS             complement(11620..12243)
                     /gene="yyaC"
                     /locus_tag="PLU15_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOO30473.1"
                     /translation="MDSSFDAMAETCQPALAHREKTPFRAHVDTPDVSSSLAQALLAN
                     LGAPPGPNAPSLIIVCVGTDRSTGDALGPLVGSNLAERQIIHESVKVIGTLENPVHAG
                     NLSDIIVQIRPAQPGRCRLLAVDACLGKLGSVGYITVAEGPLKPGTGVNKDLPPIGDA
                     HITGIVNVGGFMEYLVLQNTRLSLVIRMAQAIAGGIVNYLTSEDQKT"
     gene            13287..14717
                     /locus_tag="PLU15_05820"
     CDS             13287..14717
                     /locus_tag="PLU15_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOO30474.1"
                     /translation="MRIDTSGKRKTILLALIFVAAVAAGMCLGARGAVVAALFDNERI
                     CRGVSIDGVSVGDLTVSEARSRLEELVREYEESPISMRCQDAIWRVNPGDLGVRIEFE
                     AALRAAHSLGRSGHFCERIAERASISRSGYNISWTVTVDEQLLRDFVFAMAQQVETAP
                     VDARLVIHDDDRVSIEPDSDGWLLDTVEFINALRKATTSRSPRELEIPVRRMPAKCST
                     ASIEAKGIRRLISAYTTKFDARKTNRVNNIRLGAQKLNNRMLAPGEVMSFNDVVGPRI
                     PERGFMEADIIFDSQLVPGVGGGICQVSTTLYNAALLGLMEPVSRTNHSLPISYVSMG
                     RDAAVSYGSIDLKIKNTTAHYVLIRSYVGSDTVSFKIYGDMPDNMDVSIYTEVVERLE
                     PSTIERVDYLAPPGSVRVESDGKPGYVVSVWRIIKVDGVEARRELISRDRYKPQPKVV
                     VLGPTAAPTATEPEPSLPSIVDPPQG"
     gene            14748..15245
                     /locus_tag="PLU15_05825"
     CDS             14748..15245
                     /locus_tag="PLU15_05825"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOO30475.1"
                     /translation="MTRVLLAVEIASFILMGFVIGSYVDSKQGTRPWGTIIGFLVGLF
                     LGVMSVRRAIDEISSRAVAGEGPKVVKVVRRPISVRADEASGDPKAFYYRGTEHKVEA
                     VMSKWRDFTSSDKAMLGGKRKERDWWLNGCGKVNYRIRTEDGRVFDLQYDERKSEWIL
                     DKFLE"
     gene            15218..15829
                     /locus_tag="PLU15_05830"
     CDS             15218..15829
                     /locus_tag="PLU15_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOO30476.1"
                     /translation="MDSRQVSGMTCCEKETDNGDGGSKSASLWDMFWTFFRIGAFTIG
                     GGYVMVPLIQKDVVDRKGWVTKDEFIDALAIAQTAPGPIAVNTSVYVGYALRGFAGAA
                     ASVAGCIIPSIIIILMIASVFDRISSLDVVQAIFSGIRPAVAVLIASAAIKLGKPVIR
                     SAGELAIAAAAAIMVSLLHISPALVVVIAALAGLLTGIGGDSR"
     gene            15826..16347
                     /locus_tag="PLU15_05835"
     CDS             15826..16347
                     /locus_tag="PLU15_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOO30477.1"
                     /translation="MNLLHIYLSFAKVGLFTFGGGYAMLPLIESDIIQKRGWLTMAEF
                     TDMVAIAEVTPGPIAVNSATFVGYKLAGIIGGIVATLGVITPSIVIVLALAALVRKYE
                     RTATLSRIKVGMRPAVAGLIAAAAISVAKVSMAEPRSYAVAAAAAVMVFVGKIDPILV
                     LAVCGILGALIFF"
     gene            16653..18155
                     /locus_tag="PLU15_05840"
     CDS             16653..18155
                     /locus_tag="PLU15_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOO30478.1"
                     /translation="MRGGKGGTFPEIPEMHRQISMIVSLATFEHLPKRVGGLAEAATS
                     IGESLARENEVMVFMPSHGIHKTEKTLKIRKYGKFALDAGSASTQITMYEMERNGVRV
                     FLLSDEVMDHPDVYMPREMLTAKILRFAYAFPAAINLIMGKEGKKPDVAHINDWHCVL
                     GGAAAKKYLEIPMAYTIHRICREKIAVKDMEDAGLSEFIDPESIEISGKREMFNLETY
                     GCRVCDHLNTVSYSYLCEEWDEFFGKYAGKATYVWNGMDHSFWDPAKLERAGMPRRER
                     RAALLKENGMEDGTLYFYVGRFDMEQKGVDHMFEAIRMIFDGEVAGADRLIPNLRVVV
                     LGSGDPVLEAMARDMEKKYPKNVRAIIGYLGRETTREYYGASDFCLIPSNFEPFGLVQ
                     LEAMCMGSIPIGTRVGGINDTVLDIDKAKENATGKLVSSRKPKELAAAMVKMGILDMD
                     DPSKIEKIRANGRPHVMTNFTWDRAAQRYLAMYQGKASIMLPFADYGGPF"
     gene            18174..19181
                     /locus_tag="PLU15_05845"
     CDS             18174..19181
                     /locus_tag="PLU15_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HOO30479.1"
                     /translation="MPEFRQDIITGNWVIVAKERAKRPEDFSEPEKPRKERFRENCPF
                     CVGNEKQTPPEVYAIREEGAEPDTPGWKVRAVPNKFPALTSNGQVNTGDVGIYRTMSG
                     IGAHEVILETPDHDRSPAYLTEEEIADVIATYRHRHRVLLEDERIQYVSIFRNHGRIA
                     GASLEHPHSQIIATPIVPPLVQHSISSAKDYHDRTGRCVYCHVLEEERKAGVRVVYSN
                     PGFIVFEPFASRTPFETWIMPLRHSPSFGDISDQEIEQLADALGLTLRKIAQGLNDPA
                     YNYMIRTAPTVSEYDGAEYLHWHISILPKLAIAAGFEMGTGIYINTATPEDTAKFLRS
                     I"
     gene            19325..19660
                     /locus_tag="PLU15_05850"
     CDS             19325..19660
                     /locus_tag="PLU15_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOO30480.1"
                     /translation="MSQWLGHIVRLVVSAIVLMLISSLIPGFASLTFGTALVAAVVIT
                     VLGWLVEAILGHGVSPYARGIVGFLVSAVVIYATSFIVPGMTITVAGALLAAFVIGLV
                     DMFVPTTLR"
     gene            19796..20731
                     /locus_tag="PLU15_05855"
     CDS             19796..20731
                     /locus_tag="PLU15_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOO30481.1"
                     /translation="METEALSSSAVDDIVAGGFSKQALTDKTVVILSSLIKIGTIVLI
                     SALILRISFRVIDRLFRSREDEHIALFKDTRRLVTLRTVSKSIVRYVVYFIGGIMILS
                     ELGVDTASLIAGAGIVGLAVGIGAQNLVKDVITGFFVLFEDQYAVGDYIAVAGVSGVV
                     ESIGIRVTRIRDFAGQLYIIPNGSIGMVTNYRGHNMRVLFDVIVPYSTDIEHALRVLQ
                     KAFDEQKASIPGIADGPRVLGVQEMNETGVFLRVWATAKPMEQWSVERQLKMIARKAL
                     DDAGVDPPVTHQRIIIDRFSDPVGSQAEEGGECEA"
     gene            20728..20955
                     /locus_tag="PLU15_05860"
     CDS             20728..20955
                     /locus_tag="PLU15_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOO30482.1"
                     /translation="MSYRLGDIVRMKKPHPCGSSTWEILRVGADFRIKCTGCGRVVML
                     TRAQFMKDVRGIADSGAGGDVHAESGNRGPA"
     gene            20924..22159
                     /gene="ychF"
                     /locus_tag="PLU15_05865"
     CDS             20924..22159
                     /gene="ychF"
                     /locus_tag="PLU15_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOO30483.1"
                     /translation="MLNLGIVGLPNVGKSTLFNALTRGRAQVANYPFTTVDPNMGMVQ
                     VPDNRLDAVAAVAGAQKVTPAAIRFVDIAGLVRGASRGEGLGNKFLSHIREVDAVVHV
                     VRCFFDTDVAHVYNRPDPVRDVEIIETELILADLESVDKRIAKTEPLLKTGDKRCQFE
                     MEVLTSLKKHLESGLPARQAQLDAEEREVVDELFLLTMKPVILVANLAEECAEQLGEL
                     LPMNSISGSDASRVVPNDVASLHALEDGDGGAKGTGEGSNSGIIGEIESILDALSSVA
                     HGAPVIPVCAELERQLSELPPDDASDYLASLGLAHSALDSLIATGYSILDLITFFTIK
                     GPETRAWTVARGTVAPKAAGKIHSDMERGFIKAEVIQWDELVGEGSFVAAREHGKVRV
                     EGKDYVMQDGDVVLFRFNV"
     gene            22297..22584
                     /gene="rpsF"
                     /locus_tag="PLU15_05870"
     CDS             22297..22584
                     /gene="rpsF"
                     /locus_tag="PLU15_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOO30484.1"
                     /translation="MRAYESMFVIAPTVPEEEIDAFIDRFVQLIVNNGGEVTEIDKMG
                     RRRLAYEIEDFTEGYYVVLYFNAQPDAVAELERVYKITDNVIRSMVVRKDS"
     gene            22624..23076
                     /gene="ssb"
                     /locus_tag="PLU15_05875"
     CDS             22624..23076
                     /gene="ssb"
                     /locus_tag="PLU15_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOO30485.1"
                     /translation="MMNRVVIVGRLTHDPELRFTQNGIAVTRFSLAVDRPFTNQQGER
                     ETDFLDVVVWRRQAENVAAYLKKGSLAAVDGRVQVRSYETPEGQKRKVWEIVADRVEF
                     LDRRGDTGSQMSQGGYGRTQPELGEDEDDVPMPEEAETFGDQIDPDIR"
     gene            23120..23350
                     /gene="rpsR"
                     /locus_tag="PLU15_05880"
     CDS             23120..23350
                     /gene="rpsR"
                     /locus_tag="PLU15_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOO30486.1"
                     /translation="MRRERGRKSRRRVCSFCVDKVQDIDYKDTARLRRYLNERGKIIP
                     RRISGNCAKHQRQVTLAIKRARQMALLPYTAD"
     gene            23546..23860
                     /locus_tag="PLU15_05885"
     CDS             23546..23860
                     /locus_tag="PLU15_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOO30487.1"
                     /translation="MPYPRYTDIACGVKTIDWLEAEILGSIASLYRSLARADEGPRVA
                     DLANAIIGCYLLGKRLGISYAELEQQISANIDENIEKGHEIERWYGDFSALANHRKGR
                     ST"
     gene            23857..24813
                     /locus_tag="PLU15_05890"
     CDS             23857..24813
                     /locus_tag="PLU15_05890"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HOO30488.1"
                     /translation="MSQSRNQNLRALTEGALLAALTVVLYVADVYTRILIYVVPVPIA
                     ILVVKHDLRTGLMAAAVSALGVGLILGPIDSVIVFVRVATLGLTLGVCLAKLLPWPRT
                     IAITSVASTVQIAIDFMLAAVVSKLSPTEFLGSMMDEFAAAGERAVEIYEGMGMGETS
                     MMLARQSVELMTVLLKSFLPVIVLSAAVLGAVIVAAVARWVLVRMNEKVVPVPPFARW
                     QLGWEWIWGLIAGILLSQLGQWMGIELISTIGKNVTMGFALLYTVQGVAIIWHFFTKR
                     NIPKALAVIVIILIYLTPPFNLLVPIAGVLDTWLDFRNLAAQ"
     gene            24888..25340
                     /gene="rplI"
                     /locus_tag="PLU15_05895"
     CDS             24888..25340
                     /gene="rplI"
                     /locus_tag="PLU15_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOO30489.1"
                     /translation="MKVVLLKDVPGLGKMDTALDVAEGYARNYLIPRGLAIPATSKEL
                     NRVNKLREAEKRKGVRLEEKAREDARKLKAQGVTIRAKAGETGRLYGSITSKDVADAI
                     HESLGMEIDRRKIELADSIRELGTFTATVRLFPGISADITINVIDEGK"
     gene            25394..27376
                     /gene="lonC"
                     /locus_tag="PLU15_05900"
     CDS             25394..27376
                     /gene="lonC"
                     /locus_tag="PLU15_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HOO30490.1"
                     /translation="MKSLVKKFTKIGSRNLADKLQDDEQLVRQVMALFEILSDIYGPD
                     RLVLKAGKLDALSLMRSAELDDRILALERIVFEDPTIDRKPSPEEYESVLADIEEEIA
                     DVIARRRVEDKIEKQISDRMQQRHEDYVNEIRLQILKEDSGPDTEDTLRKLAELEELE
                     KRRLAKSALEIVRPTAMSEIVGQDRAVRALLAKVASPFPQHVILYGPPGVGKTTAARL
                     ALEEAKKVALTPFGKESRFVEVDGTTLRWDPREVTNPLLGSVHDPIYQGAKRDLAEGG
                     VPEPKLGLVTEAHGGVLFIDEIGEMDPLLLNKLLKVMEDKRVKFESSYYDPSDPNVPS
                     YIRRLFEEGAPADFVLIGATTRDPSELSPALRSRTAEVFFEPLTQVDIENIVSNAAGK
                     LGVEMDPEVPSLVSDYTIEGRKATSLLVDAYGIALFREMDNRQDGKANEAASGDGPVR
                     ITLADLEETIRLGRLSPYVNVRASDSAEVGKIFGLGVAGFLGSVIEFEAVAFPAREEG
                     KGTIRFNETAGSMTKDSVFNAASVFRFLTDRDLADFDVHVNVVGGGDIDGPSAGTAVL
                     LAILSSVYACPIRQDVAVTGELSIQGKVRAVGGIFEKIYGARQAGIKKVILPAENVKD
                     VPPGITGIEVVSVSDVVDTFKHVFEGDAIFGELRRE"
     gene            27383..28741
                     /gene="dnaB"
                     /locus_tag="PLU15_05905"
     CDS             27383..28741
                     /gene="dnaB"
                     /locus_tag="PLU15_05905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOO30491.1"
                     /translation="MATADVVGVDRVPPQSLEAEQSTLGSMLLDKEAIATAAEILVAE
                     DFYRDAHRVIFDALISLFNKGEPADLITVTEALRQRNALENVGGASYISTLANTVPTA
                     ANCEYYAKIVKNKSIMRSLVTAGSQIAALGYDQTNEVAESLDKAEQLIFRISQRGETG
                     TVSDMKTVLMSTFDRIERLYTTKGAITGLSTGFTEVDNMLSGLQPSELIVIAARPSMG
                     KTALALNIAEHVGANEGKPVLIFSLEMSREQLAQRMLCSQATVDGQRLRRGNLSEADW
                     QRLSHAIGRLSEAPIFIDDSPTVTAMDIRARARRLKAEHGLSLIIVDYLQLVQGHARA
                     ENRNQEIAEITRSLKTLARELEVPVVSLAQLSRAVEATADKRPLLSHLRESGEIEQSA
                     DVVAFIYREDYYKPDTERRNIAEIIIAKQRNGPTGVVELVWQREYTRFRNLERFAQGE
                     SA"
     gene            complement(28749..29966)
                     /locus_tag="PLU15_05910"
     CDS             complement(28749..29966)
                     /locus_tag="PLU15_05910"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO30492.1"
                     /translation="MDYAKRAQRLEPERAFSVLKKVSEMVASGHDIIGFHIGEPDFTT
                     PENIKTAACSSIHANRTHYSPPSGVPAFRSAIADYISRTRGISVSPEQVVVAAGTKLA
                     IFCAIMSCVESGADVLGPVPSYPAYPSIVSMIGANFIPVPFIDTDQGFRLDLDRLASL
                     VTPNTSMIILNFPHNPTGTLLPREDLARIAEIAIEHDLWVLSDEIYSRLVYDGDFVSI
                     SSLPDMAERTILVDGFSKAYAMTGWRLGFAVVNTELAALFDTFMANAHSCTATFTQYA
                     GIEALTGPQHEVEKMRLKFLKRRDLVVTELNRVPGFRCHMPLGAFYAFPNVTEACRMA
                     GLADADQLQDALLDDAGVAVLSMSAFHSPNGPKDDQYIRISYAAGEREILEGTRRIRD
                     FMTSRIGRSGRHI"
     gene            30042..30824
                     /locus_tag="PLU15_05915"
     CDS             30042..30824
                     /locus_tag="PLU15_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30493.1"
                     /translation="MEKSILVTAAVAALFSLLSIPGYAQDLPRGIDIARVTAERKPHV
                     LLVHGWLATSLDMSVLKYKLEQAGFECETINFSYLGAVSSIADYAVELARKVEVDCAG
                     KADVAIVAHSMGGLVTRYYLDNLRQTAQVSLVITIGTPHHGTLSGKIAPTASRCAREM
                     APGSELLRLLNAQEGDCPGVEFHAIWTPTDGVVIPAENAIMCGAKNYQVTGFGMAHMT
                     MLASDTVFEIVHAILRGQDVDVSGPQLRDHTEAEKPVVFPWQ"
     gene            30931..31006
                     /locus_tag="PLU15_05920"
     tRNA            30931..31006
                     /locus_tag="PLU15_05920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30964..30966,aa:Lys,seq:ttt)
BASE COUNT         7327 a         7137 c         9566 g         6996 t
ORIGIN      
        1 agattgcgta gccctgtaca ggctgtataa aagcagcctt tgacaaattg aacaatgaga
       61 tgggagtgcg gttacgtgaa ggtgcccctc ttcccccagg gggagccaaa aaccgccttc
      121 tgacacctat ctcggcaaat gatgctcctt cattgccagg gaggaagctg ctcttgctaa
      181 actgtctaac tcgcctgcgc gggaaatccg ctacaaccca aatcacgcgt ccttgcgatt
      241 ccttgacacc aatatgggcc tagcatataa tttacgcggt atgctgccgg tgagcttgca
      301 gggaggataa gttgagatga aaaggacata tcagcctaaa gttaggcgtc gaaaccgagt
      361 ccatggatat aggaagcgca tgagcacgcc agcgggccgc aacgtgatga ggcgccgcag
      421 ggcgaagggg cgcaagcgac ttacggtgta ggcgcagggc gtcgctggtt tctggccgtc
      481 gtcggacggc ttttccttta gtggacactc cctattaaat gcagggtgtt ctgtcttcat
      541 tgggctgtta ggaggcccgc caatgccaac agtgagtttg cgtcgaactt cagattttca
      601 aagagctttc cgcgaaggca agagcgccgc aggacagtat gtagtggtgt acgctgtgcc
      661 taatggcttg cctgaagttc gcctggggta ccctgtggga aagaaactgg gcggcgctgt
      721 caagcgtaat cgtataaaga ggctgttgag ggaagctgcg cgcctcgcgc ccaacatgcc
      781 tggcatggga tatgacatgg tcctcgtgcc gaggcgaaag attgcagatc cgaacgtgcg
      841 ctgccataat gttattggcg atttggagaa ggttcttgac agactatcgc tcgaaactga
      901 gttaggagag ccgagaagct gtggcggatc ggatgtatga gggttggagg ggcagcaaat
      961 gtgggcagtc cggatcgagt tagggttgat tcgtttttac aggacatgca tatctccgct
     1021 taaaaggtat ccgtgttgtc gctatgttcc aacatgttcg cagtatgctg aagaagcggt
     1081 caagaagtac ggtgttattc gaggcacatg gctggctatg aagagactgg caaggtgcca
     1141 cccgttcagc gctgggggat acgatcctgt caagtaggcg tgatgaacaa atggcgacag
     1201 ctagggcaga aagccaagcc taagtgcgaa agggggatcc gccagtgggc atcactggag
     1261 tgatgcagag cattctgcga ttcttcgcag aaggaacagg caaccttgca tgggcaatca
     1321 tcatagtaac gactttaata agagctgcgc tccatcctct gcaggttaag caggtcaggc
     1381 taatggagca gatgaaagag attacgccga aagtgcaaga gctcgagaag aagtacaagg
     1441 ggcagcctga ggaataccag cgtcgggtgg cggaattgta ccgtgacaat aatgtaaacc
     1501 cggcggtggg atgtctcagc atgcttattc cgctcccgtt ttttgtaata ttctaccgcg
     1561 ttttgcaaga caccgcgttc attggcggat tgccggaggc tgcgaaggga tttttatgga
     1621 taaaagattt cgccctcccg gacccatggg cccttcctat tttatcagct gtaacaacat
     1681 ggctttcgag caggcaaacg gctacggatc cgtcacagaa gtccatggcg ataatcatgc
     1741 ccatatttat gggctggatt gctatgagaa tgccggccgg cgcggcgctg tattgggtca
     1801 cgaacaatct gatcagtgtg gtccagcagt ggttgatcca gcgtcaactg gcggcggcca
     1861 agaagggagt cagctagatg gagcctgtcg aagcagtagg caagactgtc gaagaggcag
     1921 ttgagtcggg tttgtccact ctgggtttgg caagaagtca ggttgaaata gaagtcatag
     1981 atgaaggatc taagggattg tttgggcttg tcggaaggag acacgcaagg gtcgttgtgc
     2041 gcccacttgc caacatagag agcaagggag aggcttatga aggcgccatg ggcgcagtcg
     2101 agtctcccgt gagggctgaa gaggatttgg gcggcatcga cgaagatgac gatgaatgga
     2161 gaggcgggtc cagtttcgac gaggacgatg ctaaaagcag tggatatgtg ttcttggagg
     2221 agctgatctc cctgatgggc gtagatgttg acatcacgga gacccggtct gaagatatta
     2281 ccctgctgga agtggagggc agatctcttg gcatgctcat tggcaggcga ggagagacgc
     2341 tcgatgcaat acaatatctg gtaaatttgg cagccaacag ggaggctcgg gcgtccggaa
     2401 gctcggagag ggagagatac gtagtcgata tcggcgggta tcgcaagagg cgagaagagg
     2461 ttctcgagaa caaggctttg gagcttgcag aaagggtagt gcaggagcaa agggacgaag
     2521 tcatggagcc gatgtctgcc cttgagcgac gtgtagtcca catggccctc agagactacg
     2581 atggcgtcgt tacgcactcg gaaggccggg agccatatag gcgaatagta atatccccca
     2641 aagagtgaaa aggatcgagc gtatagaggg tgacgatgaa taggagtgcg tgttttaatg
     2701 acgcgcactt tcttttatgc tctagatgag gaagcccgct gcgggatttg gggatgccga
     2761 tcgacttggt tgacactcac ggagattgtg cgtgtggtta gagtctgaag gaagggggaa
     2821 gctcagatgt tcagagagga tacgatagcg gctattgcca cacctcccgg aattgggggg
     2881 atagccgtaa tcaggatatc tggtcccgaa gcgaaagatg tggcccagcg tgtttttgtg
     2941 cctgctcggc caggtgcggc gtcagacggc gcggctgggc tgtatcctat ccgagggtgg
     3001 ttatgcaagt acggacacgt gcatgacgcc tcaggggagc taatagacga agcgataatg
     3061 acctggatgc ccgggcccac atcttatact cgcgaagatg tggctgagat aagttgccac
     3121 ggaggaactg ctgtggcgcg cgcggtcctc gaagctactc tgaaggcagg tgcgcgcatt
     3181 gctcaaccag gcgagttcac gcggcgggcg tttctgaacg ggcggatcag ccttgagcag
     3241 gctgaagcgg tgctttccat ggtaaatgcc cgaacagaga gggctaggaa agcggcctcc
     3301 tcatttctcg ccggatccct tggcgtgaaa gtgcgtgaag ccgaagagag catcatttcc
     3361 ctgatgacgg tgattgaggc atcgctcgat tttccggagg aggatatacc cgacgttgcg
     3421 gtgcaggaga tcgcggatga atcgtcgcgg atcgctgcca tgctggagga ggcgtgccag
     3481 gaagcatcta tgggacgtct gctttgggaa ggggcctctg tggccatagt tggaaggcct
     3541 aatgtcggga aatccaggct tctcaacgcc ctgctcaagg aggagcgggc tattgtgtcg
     3601 gaattgccgg ggacaacgag ggatgtggtg tctgaagttg ccaatatccg cgggatccct
     3661 ataaggttga tagacacagc cggaatgcgc gattcggtcg atccggtgga gcgaatcggc
     3721 gttgaaaggg ctaagatagc tctggcagat gccgacgtgg cacttgctgt tgtggacggg
     3781 tctgagccgc tcgacatggc cgacgcggag atcgtgtcgc agacaccgga atcgcatacc
     3841 atcgtcgtcg taaataagtg tgacctggct aacgtcctgg gcgatgagga tgcgcgccga
     3901 atcgctgccg gacgtcctgt cctcagggtt tccgcgctta ctggcgaagg gcttgacaac
     3961 ttggaggagg ccatcgaggc attggtgctt ggggatcggg gtgcgggtat agctgagatt
     4021 ggcgcgttgg ccggacaagg gctgcatggc ggtcaagccg cagatcaacg tgatgggctc
     4081 gaaacagaat ccgaaatgct cggcatgaga cgcgaagatt gcttgcggcg cgcggcacaa
     4141 tccctcagag atgttgcctg cggcgtggag gagaacatgc cgcccgacat gctgtcgatt
     4201 gatttgaggt cggccctgga tgcgctgggg gagcttactg gcgaaacaac gagagaggat
     4261 attgtggata ggatatttgc ccagttttgc gtcggaaagt aggagtcgaa tacggttgtg
     4321 cctgaccgca tgggcgatag gcggcatgtg agagacgggg ggtgaatcgg aaatggatta
     4381 cgatgtcata gtgattgggc tggggcatgc aggttgtgaa gcggctctgg catgcgctcg
     4441 gttaggatgt agcacgcttg gattgacggt gagcatggag aacatagcgc agatgtcgtg
     4501 caatccctca ctaggcggac ccgcaaaagc ccatctcatt cgggagatag acgcgttggg
     4561 gggagaaatg ggtcttgctg cagatgagac ctttatccag ctgcggatgt tgaatacttc
     4621 aaaaggcccc gctgttcgtg ggttgagagc gcaaatagac aaggccgagt atcaggcgag
     4681 gatgaggcga acgcttcagc gccagcccgg actggtgctc acacaagcta cagtgtatga
     4741 catcatcgtg cgcaatggaa gagtagtcgg agtgagcacc gagatagggg agatttcgtc
     4801 ccgggcagtg atccttgcta caggaacaca cctggggggc agggttcaca tgggggagat
     4861 gaactacgag tcaggccccg acagacagcc ggccgccgtt gagctgggcg aaaacctgag
     4921 gcggcgagga ttcgccatgt accgcttcaa gacaggaacc ccagccagaa tagatgggag
     4981 gaccgtggat tacgatgcga ctcagatcca gttgggtgag gaactcccat acggcttttc
     5041 gtatctgcga cggccctatc cgagagagca gatcccatgc tggcaaacgt ggacaaacga
     5101 aagaacccac gaagtgatca gggagaatct tgatcgtgcg cccatgtata ccggcgcgat
     5161 cacggggaca gggccgcgat attgccctgc gattgagaca aagattgtga ggttccctga
     5221 ccgccctcgt catcaggttt atgtggaacc tcaggggcgc ggaaacgagg agatgtatct
     5281 gtccggtgtg tcgacaagcc ttcctgtcga tgttcaggag aagatgatca ggacaatacg
     5341 tgggctcgag aatgtgcaca tcatgaagta cggttacgcc atagagtacg actgcataga
     5401 cccgactcag ctggcgccta cgctcgagtc cctgaagata gcggggttgt atttcgcagg
     5461 gcagataaat gggacctccg gttacgagga ggcggccgcg cagggacttg tggcgggaat
     5521 caatgcggcg ttgagtgtcc ttcgccctga tgagcggctc ttgctcgata gatcagaggc
     5581 ctatattggc gtactcattg acgatctggt ctcgagggga actgatgaac catatcggct
     5641 gatgactgca cgggccgagt acaggcttct tctgaggcag gacaatgcag atcttcggct
     5701 tactccccgc ggaagggaga ttgggctagt cgatgaggac cggtgggaaa ggttcgctgc
     5761 aaggcgtaac gccattgagc tgattcgcag tggcgaagag cttcctgagg ctctcaggga
     5821 ttatgctgaa gaggcgaccg aacaggtgga gatagaaagc aagttttcag gttatattgc
     5881 caaacagcgc cgtcaggtgg aagagttcaa aaagcttgag aaatggaaga tccctgagga
     5941 catcgattac accaaactga cgaggctatc gcgggaagcc cgtgagcgcc ttggggttgt
     6001 tcgaccgttt tcggttggac aggcatcgcg cgtgcccggc atttcccccg cggatctgtt
     6061 ggctctcacc gttcacctca gagagatgac tttgcaggga cgaaaagagg aggcagcgag
     6121 ggacaatgga tgagtttgtt tattacctga aacagggggc ttgcgagctc ggcgtggctc
     6181 tctcagagcc tcagatcgag atgttttgtg cttacaaaac aatgcttgtc cacgccaatc
     6241 gctttgtcaa cctcacatct atcacggatg accaggggat tgccgtcaag cacttcgttg
     6301 attcgctcag ttgcctgctc gtggcgggca cagagaattt caatagcttc gtcgatattg
     6361 gcactggcgc gggatttcct gggctggtgc tcaagatcat gaatcccgga gcccgtgcgc
     6421 tccttgtgga cagtgtaaga aagaaagtgg atttcgtgcg cgaggtagca gattctttgg
     6481 gcctcgatgg ggtggaagct ctgcatggcc gcgcggagga cttcggtcag gatggagaat
     6541 accgtgagca attcgacctt gctgtggcgc ggggagtcgc caacatgggt gttttggcgg
     6601 aatactgcct ccctttcgtc aggccggggg gcctttttgt tgcgatgaag ggtccgcggg
     6661 tagtcgatga aatggatgcc gggggcaagg ccatcgaaat actagggggc agccaggcaa
     6721 tcctccgtca ccttgcgctt ccttacgacg ctggagaaag ggtgttagtc gcggtgagaa
     6781 aagagcgcaa tacgcctgcg aagtatccgc gaaaggcggg agcgccggcc agaaaacctc
     6841 tgggtgatgc ataatcacac tttcgggtga tactgaatcg cccactatag tgaaggacat
     6901 tcttgcggcg tggcgaagtc atgaacagca ttctaaggag gcgtaacgca ttgggcaggg
     6961 tcattgcagt agccaaccag aaaggcggca cggcgaagac tacgaccgca gtcaacctcg
     7021 gtgcctatct tgcgatggca ggcagaagaa ttcttttggt ggacatggat ccacagggaa
     7081 acgccagttc cgggctgggt gttgacaagc gggccgtgga gtcatgcatt tacaatgttc
     7141 tgatcaacga tattgagatt gaaaaagcga ttctcaacac gggagtccag gggcttgaca
     7201 tcgttcccgc tacgctcaat ttggcggggg cggaggtgga gatggtcagt ctcatgtcgc
     7261 gtgagcacag gctcagaaag gcactcgagc cggtgagggt catatacgac tactgtttag
     7321 tggactgtcc tccgtcgttg ggcctcctaa cagtaaatgc gttaactgct tcggattccg
     7381 tcctggtgcc catacaatgt gagtattacg cccttgaagg cttgggtcag ctaatgcgga
     7441 ctatcgattt ggtgaaacgt cacctcaacc cggagctaga gatagagggt attgtaatga
     7501 ccatgtacga tgcccggacg aatctctcgc aacaggttgt tgaggatgtg aagaataatt
     7561 ccggacagca cgtttttgag gccgtgatac cgagaaatgt gcgcctgagc gaagctccga
     7621 gctatggagt tccgatatct atgtatgatg acagatcgaa gggtgccgag gcgtatagaa
     7681 gtctggccaa cgagattatg caaagggagg gggcttagcc atggcgacta agcggggatt
     7741 gggcaagggc ctcaatgcaa tcataggaga ggccatgctc aatgaggagt cgcatgaatt
     7801 acggggtatc gagataccgc tggatcagtt gaccgcgaac cccttccaac caagagccga
     7861 gtttgatccg gacaagttgg aggaactggc tgaatccatc agggcacatg gggttctaca
     7921 gcctatagtg gtaaggcggg ctggcgagaa ctatcagatc atcgccggag agaggcgttg
     7981 gcgggccgca cagatggctg gtttgcagac tatccctgcg gtcgtcaagg aacttgacga
     8041 tcctggaatg atacaggtgg cgctcattga gaacctgcaa agggaagact taaacccgat
     8101 agaggaggct tcagcctatc ggaagctgat ggacgaattc agtatgacac aggaacagct
     8161 atccggcact ttgggcaaga gccgatcgac aatagctaac gctgtgagat tgcttaatct
     8221 ccccgacgaa atacaggagc atgtctcacg tgggcgcctg acaggagggc atgcccgatg
     8281 cctcttagcc cttgatgacg ccaagttgca gctggaggta gcaaaggaag tcatcgataa
     8341 ggggctgacg gttcgtcaaa cagaggaact agtgagaagg ctagtgaaaa atgtttcacg
     8401 tgaaacaatt gaacaggctc cacaggtgcc cgacccggat gtggtagcta tcataaggca
     8461 cctgagcgaa aggctcggga cgaaggtgaa aataaccggg tcctcgggca agggcaaggt
     8521 agaaatagag tactattcag aagacgatct aggaaggatt ctcgatatca ttcttggcat
     8581 tgcttgacgg caacgccggt ggaaggaaag ttgatggact ggaggaggga ccggggcctc
     8641 gtgtggcctg ggcttgatat ggaacaaagt gcagtaattg gcctcatctg tgcacaacag
     8701 cagctcgtgc caatgattct atcaattatt ctattgataa gcgtggttgt ttttatcaat
     8761 gcaacgaaca ggatcagacg tctggagaag gcataccgct ctttgctcgg gacggagagc
     8821 gaagagaagg atatagaaca gataatcatg gaatatattc gcaagacgga catgcttcag
     8881 gaccaacttg atgagactcg aaaggaattg gagcgcgcac aagatgcggc tctagcttac
     8941 atacagaagg tcggcattgt tcgatatgat gcttttgacg atacaggcgg agagcaaagc
     9001 ttttccatgg cattgctgga cgccaacgct aacggtgtcg tcgtcagtgg gctgtcgggg
     9061 cgtgaggaga ctcgtgtcta tgccaaaccc gttaagggcg ggcgttcccc tcatacgtta
     9121 tctgatgagg aaatacaggc tattgctaga gccattgagt catccaaaga gaaactatag
     9181 tgcccaggaa ggcgaactga acgaaagtcg ggaaatcggg aatcattaat ggaagatgtt
     9241 gcaggaaatc ggattcatat ggcgaatatg tccgtgatgt tggtgtctgc cagggggcag
     9301 acttcttctt gagtcatctt gaacgggttc gaaagggggg cgaccggtgc agcaaaggtt
     9361 cattcggcga gttcctgccc ggaagcgcca agggtccctt acagtcatcg ccattccatc
     9421 ctcgggagaa cctccaaggt cggttggcat tccgacgcag atgctcgcga tctgcgctgc
     9481 agcactaata ggggtaattg gcatattcgt gatctcgtat ttgaacatga gccgagggat
     9541 tgcgacgctc aatgaggcaa agatggacag cgacaatgcc attgagcaat tgctttcaga
     9601 gaatcagacg ctcaagcttg agaagcaaga gcgacttgcc aaaatgcagg aacttgcgag
     9661 caagacacag atgctggaag cgacagtgag ccggattgct gacgagaagg cggagatatg
     9721 ggcgatagtg aaaggttcgg atgcggccca aaagaacgct tcatcaagca atgcggtttc
     9781 atctaggggt ggagcttcga gaggtggcgc tcccggggca gaaagggaat cccctgaggc
     9841 ggtgctcgat gccgtggcaa atgccccggc cgtagatgtc ggcgccgacg ctttcgatgg
     9901 agagcagtat cttgtggaga cgatggcaga agaacttgaa atgttgttgg ttgcaacgtc
     9961 ggcgatgcaa aaagatctgt cggacttgcg cgtctccgct gagacctatc gctccaagtt
    10021 ggaccacacg ccgtctggat ggcctgtcaa cgggcgaatc acatcagcgt acggcaacag
    10081 gcggcatccg atactgcgga tcactcgctt tcatgagggc gttgacattg cggcgacgca
    10141 tggcacgcca gtagtggctg ccgccgatgg agtagtagtc ttgtctggct ggtccggcgg
    10201 gtatggcaat gctgtagaga tttcacatgg cttcaattgc agcacctttt atgcccacaa
    10261 ctcgaggaat gtggtgaagg tagggcagaa ggtgaagaaa gggcagataa tcgcctatgt
    10321 gggcagcact ggcttgagca ctgggccgca ctgtcattat gaggtgcgaa taggagggcg
    10381 tcacgtggat cccgcgccat acatgaaata gagcgcttta gcacagactt agcaggaggg
    10441 gatcgcatgt ttcgtagaag gccggagaag gcgcctggcg cggcaagaat tgatacttac
    10501 ataggcaagg gtaccacggt agagggctcc atcgaggcga gcgggtcggt gcggatagat
    10561 ggctttgtaa agggcacact gcgcgtggat ggcgacctca taattggggc cgacggcgta
    10621 gtggtggcgt ctgtaaccgc ggccaatgtt accgtagccg gtgaactcag gggtgaagtg
    10681 aatagtcccg gaagattgga catagcaccc actggccaga tgttgggtga tgtcatcgtc
    10741 ggatccttga atgtggatga tggcgctttg tttcttgggc agtgcaacat gaaggacgag
    10801 gttcggtccc aaccgaaggt cctggcagct ccagatgaca gcggaaactc cgattctcag
    10861 cagtagccgg cttgaggaaa taatgtggga tcgttggggg taagcaatcc tttggaaggt
    10921 gcttgccctc ttttgatatg cagcaaattg aatcgaatgg gagggttgtc agtgcagttt
    10981 cgtgacagag ccgaagctgg ggctcttttg gggagacggt tgcagcaaga aacatgggat
    11041 gacccaattg tcctggcctt gccgcgcgga ggcgttcccg ttgcagagtg tgtagctgat
    11101 gccatcggtg gagagttggg agtagttgta acgagaaaga tctgcgctcc acataaccgt
    11161 gagttggcca taggcgctgt agatgctgac gggaggatgt ttatagattc acgcattgtg
    11221 caggcactgg ctgtagatga ctcctacatc cgcgccgaaa catcacggca attgcaggag
    11281 attgagcgga ggatggcaga atactgcgag aatggagacg tgccaaatcg gaaggcccgc
    11341 gatgtggtgc ttgttgatga cggcattgcc acgggataca cgattctggc tgccgcaagg
    11401 tacgtgcggc gccacggtcc agcgcgtaca gtgattgcca cgccagtctg cccgcccgat
    11461 gtttttagca agctccagga agaggcggac atggtagtga gcttgctgct cccggcgcat
    11521 ttcatagccg tggggcaatt ctatgaagac ttctcgcagg tatcggatgc agatgtgcgc
    11581 aacatcttga ctcgcgttta cacaaaaggt agtcgctgat catgttttct ggtcctcgct
    11641 agttaagtag tttacgatcc cacctgctat tgcctgagcc atccgtatta ccaaggacag
    11701 acgggtgttc tgaagcacca gatactccat aaacccacca acgttgacta ttcctgtaat
    11761 gtgcgcatcg cctattgggg gaaggtcctt gtttaccccg gttccaggct tgagcggtcc
    11821 ttcggcaacg gtgatgtagc cgacacttcc aagcttgccg aggcaagcat cgaccgcaag
    11881 aagtctgcat ctgcccggct gtgcgggcct gatttggaca atgatgtcgg agaggttgcc
    11941 tgcatggaca gggttctcca aggtgcctat tactttgacg gattcgtgga ttatctgacg
    12001 ctcagcaaga ttggaaccga ctaagggtcc cagcgcatcc cctgttgatc tgtcagtacc
    12061 gacgcatacg atgataaggc tgggtgcgtt cggacctgga ggagccccca aatttgccag
    12121 aagagcctgc gcaagggaac tgctcacatc aggcgtgtcg acgtgggctc tgaagggtgt
    12181 cttctctcgg tgcgccagtg caggttgaca ggtttcggcc atggcgtcaa agctgctatc
    12241 cattttgcga ccccacacct cccttgtctg agcgccgttt cgcagcaatg gacgggaatg
    12301 attctctagg ttgcggcatt tgaagtatat gagtgtgctg ccccttatat gtggtctgcg
    12361 gggtacgctt gtccacgatg caatcgccta caggatggta cgcagaagtt ggagcgaaaa
    12421 agcgtctggc aacagaagga gaatcattcg cgcaagggga aattggcacc ttgaggcttt
    12481 tcccggactg catctatcta tagcgcggaa ctgggggatg gctgtacacg gccggccaat
    12541 agcgggaaac tagtaaagag ccggtgctac tgtcaggcgt gagctgaggg gcacgcattg
    12601 ttcttgcggc tagagtttga atcttagacc aagttaggct gccaaaaagc aatcaccgcg
    12661 gtgataagcg cattcatcaa gccatagcgc ctgttggctc taactgcagc tctcgaaagc
    12721 cgccatatct tctctttggg gttagaagct atgaaatgcg gctttacctt tgtggatgct
    12781 gtagttgacc agtataatgt gaatccgcct gtcgggtagt aggaaacgac gtattcaagg
    12841 aactcaagct aacccactct atcggattga tggtggacgc agccgccttt gcggatattc
    12901 aaaccgaagg aatccgggtt tgattactgc atagccctca tagccgccag aagaccaagg
    12961 tcgtatctta tcccggacga gaacgaggtt tccagacact atggaactcg ggggcccctt
    13021 ttgtggcgta aatcgtagcg aaacgacgtc gatgccgtct tttgatatga catgaggtgg
    13081 atgtgtgccg catggcctta actgttgaaa tctcgtaatc attttggcct caaaagcgcc
    13141 acctggccgg aggcgggcct cctttcaggg caggcctggc gcgatggttt gtgcccgggt
    13201 tcgcatggcg tgcaagggtg ttcacttttg tggtaatatt ggtcacaacc ttaccctatt
    13261 catattgcgg aggcaagaga tttcttatgc gtattgatac tagtgggaag cggaagacaa
    13321 tactcctggc gctcatcttt gtcgcggctg tggctgcagg catgtgcctg ggtgcgaggg
    13381 gcgccgtagt ggcagcgctc ttcgacaatg agcgcatatg ccggggggtg tcgattgacg
    13441 gcgtcagtgt cggagatctt actgtctccg aggctcgttc gcgcctggaa gaactcgttc
    13501 gggagtatga ggagtctccg atctcaatga ggtgccaaga cgctatctgg cgtgttaacc
    13561 ctggcgatct tggggttagg atcgagttcg aagccgccct gagggctgct catagcttgg
    13621 ggcggtctgg tcacttctgc gaaaggatcg ctgagagagc gtcaatatct cggtcgggtt
    13681 acaatattag ctggactgtg acagtcgatg agcagctgct ccgagacttt gtttttgcca
    13741 tggcgcagca ggttgagact gctcccgtag atgcaaggct cgtcatacat gacgatgacc
    13801 gtgtttccat cgagccggat tcagatggct ggctgttgga cacggtcgag ttcatcaatg
    13861 cgctaaggaa ggccactacg tctcggtcgc cacgggagtt ggagattcct gtgaggcgca
    13921 tgcctgcaaa atgtagcaca gcatccatcg aagccaaggg cattcgccgc ctgatatctg
    13981 catatacaac taagttcgac gcgagaaaaa ccaatcgggt gaataacatc aggcttgggg
    14041 cacaaaaact gaacaatagg atgcttgctc ccggcgaggt gatgtccttc aacgacgtgg
    14101 ttggtccgag gatccccgag agagggttca tggaagcgga cataatcttc gattcccagc
    14161 ttgttcccgg ggttggcggc ggcatatgtc aggtttcaac tacgctatac aacgctgcat
    14221 tgctcgggct gatggagccc gtgtcccgca caaaccattc attgccgata agctatgtgt
    14281 ctatgggaag agatgctgcc gtttcgtacg gatccatcga tctcaagatt aagaacacga
    14341 ccgcccatta tgtcttgata cgatcgtatg tcgggagtga cactgtttcg ttcaagattt
    14401 acggggatat gcccgacaac atggatgtaa gcatatacac ggaggttgta gagaggctcg
    14461 aacccagcac aatagaacga gttgattatc tggcgccccc gggatctgtg agggtggaaa
    14521 gtgatggaaa gcctggctac gtcgtgtcag tatggcgcat tatcaaggtc gacggtgtcg
    14581 aggctaggag agagctgata tctcgggatc gctacaagcc tcaacctaag gtagtagtgc
    14641 ttggtccgac tgccgcgcca acagcgaccg aaccggaacc gagtctgcct tcgatagtgg
    14701 atccaccaca gggatagaag aatggctgtg catggagctg aaatgttatg acacgtgtgc
    14761 ttcttgccgt agaaatagcg agtttcatcc ttatgggctt cgtaataggc agctatgtcg
    14821 attcaaagca agggactagg ccatggggaa cgataatcgg ctttcttgtg ggtctgtttt
    14881 tgggcgtgat gagcgtgcgc cgcgcaatcg atgagatctc gtctcgggct gttgcggggg
    14941 agggcccgaa agtcgtaaag gttgttcgcc gccccattag tgtccgagca gatgaagcaa
    15001 gcggggatcc caaagccttt tattaccggg ggacggagca caaagtggag gcggtcatga
    15061 gcaaatggag ggatttcact tcttccgata aagctatgtt gggcggaaag cggaaggaga
    15121 gggactggtg gttgaacgga tgcggaaagg tgaactacag gataagaact gaggatggaa
    15181 gagtctttga tctacaatat gacgagagga aatcagaatg gattctcgac aagtttctgg
    15241 aatgacatgc tgtgaaaaag agacggacaa tggcgatggc gggtctaaat cggcgtcatt
    15301 atgggacatg ttctggacat tcttcagaat aggtgcgttt accataggcg ggggatatgt
    15361 gatggttccc ttaattcaga aggacgtggt tgacaggaag ggttgggtca ccaaggatga
    15421 attcatcgat gcgctagcaa ttgcgcagac ggcacccggc ccgatagccg tcaatacgtc
    15481 cgtatatgta ggctatgcgc taaggggttt tgcgggtgcc gcagcatctg tagcaggctg
    15541 cattattcca tctataataa taatcttgat gatagcatcg gtcttcgatc gaatctcctc
    15601 gctagatgtg gtccaggcga ttttctctgg catacgccca gctgttgcag tccttatcgc
    15661 gtccgctgcc ataaaactag gaaaaccggt gatccgctcc gccggagaat tggcgatagc
    15721 cgcggctgcg gcgataatgg tttctttgct tcatatcagc cctgcgctgg tggttgtcat
    15781 tgctgcattg gcaggtcttc tgaccgggat tggaggagat tcaagatgaa cctcctccac
    15841 atatacctca gttttgccaa ggttgggctt ttcacgttcg gcggcgggta tgctatgctg
    15901 ccgctgattg aatctgacat tattcaaaag cgcggctggt tgactatggc ggaattcacg
    15961 gacatggtcg caattgccga ggttacaccc gggcccatag ccgtgaatag cgccacgttt
    16021 gtagggtaca agcttgccgg gataataggt ggaatcgtag ctacactagg cgtcataacc
    16081 ccatcaatag tcattgtctt ggctcttgct gctcttgtta gaaaatatga gcgcaccgcc
    16141 acgcttagta ggatcaaggt cggcatgcga ccggctgttg cgggcctgat agctgccgca
    16201 gcaatatccg tagcaaaggt gtcgatggct gaacctcgtt catatgcagt agccgctgca
    16261 gcagcggtca tggtttttgt aggcaagatt gaccccatcc tggtccttgc tgtatgcggg
    16321 attctgggag cgctcatctt tttctgagtc tgactataac gatgcaggtg cgcccttgcc
    16381 aacggaaaaa tgggctcatc acggtctcaa ggagtctcca catatcgcat ccctcccgat
    16441 gattccaggg ccgcgcgacg gctgcatttc attatatttt tcgtgcagcc gaaagtgatg
    16501 gcatggggca tattaaagca agtcagcagt ttgttgagtg cccaggatga ttcgccgctt
    16561 tacacggcct ggcagagagc gagcgtctgg aagcggcttg aaggggcggc taggttatag
    16621 ccgcgaggat cgcaaaagcg tgccaaactc atatgagagg agggaagggc ggcacatttc
    16681 cggaaatccc ggaaatgcac cgccagatat ccatgattgt tagccttgct accttcgaac
    16741 acctgcccaa acgtgttggg ggtcttgcag aagctgccac gtctataggg gagtctctag
    16801 ccagggagaa cgaggtaatg gtcttcatgc cgtcacacgg catacataag accgagaaga
    16861 cgctgaaaat cagaaagtac gggaagtttg ctctggacgc aggttcagct tcaacgcaga
    16921 ttaccatgta tgaaatggaa cgaaatggag tacgcgtttt cttgttgagt gatgaggtta
    16981 tggaccaccc agatgtatac atgccaaggg agatgctgac ggccaagata ctccgattcg
    17041 cttatgcctt tcctgccgca atcaacttga tcatggggaa ggaagggaag aaaccggatg
    17101 tggcccacat caatgattgg cattgcgtgt tgggaggagc tgcagccaaa aaatacctcg
    17161 agatacccat ggcctacacc atacaccgca tctgccgcga aaaaatcgcc gtgaaggata
    17221 tggaggatgc gggtctatcg gaattcatcg accctgaatc aatcgaaatc tcgggaaagc
    17281 gggaaatgtt caacctcgaa acgtatggct gtagggtctg tgaccatcta aataccgtca
    17341 gctactcata cctttgtgag gaatgggatg agtttttcgg gaagtatgcc ggaaaggcga
    17401 cctacgtttg gaatgggatg gatcattcct tttgggatcc ggccaaactc gaacgggccg
    17461 gcatgccaag gagggaacgc cgcgctgcgt tgctcaaaga aaatggaatg gaagacggaa
    17521 cgctctactt ttatgttggc cggttcgata tggagcaaaa aggcgtagac cacatgtttg
    17581 aggcaatccg catgatattc gatggcgagg ttgccggggc agataggcta attccgaact
    17641 tgagggttgt ggtcttaggc tcgggagatc cagtactcga agccatggcc cgggacatgg
    17701 agaagaagta tccgaaaaac gtaagagcga tcataggata cttaggaagg gaaactacta
    17761 gggaatacta tggagcatca gacttttgtc tgattccgtc caatttcgag ccctttggcc
    17821 ttgttcagct cgaggcgatg tgcatggggt cgattcccat aggcacaaga gtagggggca
    17881 taaacgatac agtccttgac atcgacaaag ccaaggaaaa cgccaccggc aagctggttt
    17941 cctcacgtaa acccaaggaa ctagctgctg ccatggtcaa aatgggcatt ctcgacatgg
    18001 atgacccttc taagatcgag aagatcaggg ccaatggcag gccccatgtc atgaccaatt
    18061 tcacatggga tcgggcggcc caaagatact tggcaatgta ccagggaaag gcatcgatca
    18121 tgttgccatt tgcggattac ggaggtccgt tttaatcaag gaggccaggc accatgccag
    18181 aattcaggca agacatcatc acaggaaact gggttatcgt tgcgaaggaa agggctaaaa
    18241 ggccggaaga cttttccgag ccggagaaac cccgtaagga acgttttcgc gaaaactgtc
    18301 cattctgcgt aggaaacgag aagcaaacgc cacctgaggt ctatgccatc agggaagaag
    18361 gcgcagaacc cgacacccct ggctggaaag tgagggccgt ccccaacaag tttcccgcgc
    18421 ttacatcaaa tggccaggtt aacacgggcg atgttggcat ttatcgcacg atgtcaggca
    18481 taggggccca tgaggtaatt ttggagacgc ctgatcatga cagaagtcct gcctatctga
    18541 ctgaggaaga gatcgcagat gtaatagcaa cctatcgcca tcgccaccgt gtgctcttgg
    18601 aggatgagag gatccaatat gtgtctattt tccgcaacca tggcagaatt gcaggagctt
    18661 ctctggagca tccgcattcg cagataatag ctacgccaat agttccgcct cttgtccagc
    18721 attctatctc ctcggccaag gattaccacg atcgaacggg cagatgcgtc tactgccatg
    18781 tccttgagga ggagcggaag gcgggcgtgc gagtggtata cagcaaccct gggttcatcg
    18841 tgtttgagcc atttgcgtcg aggacgccgt ttgagacatg gataatgccg ctgcgccatt
    18901 ccccatcctt cggcgacatc tcagaccagg agattgagca gcttgcagat gctctcggat
    18961 tgaccctgcg taagatcgcc caaggactga acgatccggc atacaattac atgatacgca
    19021 ctgctccgac cgttagcgaa tacgatggcg cggagtactt gcattggcat atcagcatat
    19081 tgcctaagct ggccatagcg gccgggttcg aaatggggac aggcatatat atcaatactg
    19141 cgacacccga ggacacagct aaattcctga ggagtatatg acgggaggtc tgttttttcg
    19201 gcccaggtgc gagcgctttc gcgctcgcgg agcaagatcg agcatagtcc ccccaatgct
    19261 ggttatccta taaccacgat tggcgaatca aaccagcaca tgtactgggg gggcagaaga
    19321 gtcgatgagt cagtggttag gacatattgt gagacttgtt gtgtcggcga tcgtgttgat
    19381 gctaatctca tctctcatac caggttttgc aagtttaacg tttgggacgg cattagttgc
    19441 agcggtggtc ataactgtgc ttggatggct ggtcgaagcc atcctcgggc atggggtatc
    19501 cccgtatgca aggggtattg tgggctttct cgtgtcagca gtggtgatat atgctacgtc
    19561 gttcatagtg ccgggcatga ccataaccgt tgcaggcgcc ttgctagcgg catttgtgat
    19621 tgggcttgtt gacatgtttg tcccgaccac cttgcgctag tagtttcacg tgaaacaatt
    19681 gatgcctgcg cccacccctt tcggcagttg gccgggaggg gtttcacttt atcaggcagg
    19741 acaagttgag gtgatgtcta acataagcta tgtgtgaaga taggggtggt cgcttatgga
    19801 gacagaagcg ctgtcatcct ccgcagtgga cgacattgtg gccggaggat tctccaaaca
    19861 ggctcttacg gataaaacgg tggttatcct atcgtccttg atcaagatag ggacgatagt
    19921 cttgatctct gccctgattc ttcgcatttc ctttcgcgtg atcgatcgcc ttttcaggag
    19981 tcgcgaagat gaacatatcg ccttgttcaa ggacacgcgc cgtcttgtca ctcttagaac
    20041 ggtgagcaag agcattgtca ggtatgttgt atatttcatc ggaggaatca tgatcctttc
    20101 tgagctagga gtcgatactg ccagcctgat tgcaggagca ggaattgtgg gattggctgt
    20161 gggaattggc gctcagaacc ttgtcaagga tgtaataacg ggatttttcg ttctatttga
    20221 ggaccaatat gccgtcggag attacattgc ggttgccggg gtctcaggcg tggtggagtc
    20281 gattggaata cgggtgacgc gtattcgcga ctttgccggg cagctctaca ttatccccaa
    20341 tggttctata ggcatggtaa caaattatcg tgggcataac atgcgggtct tgtttgatgt
    20401 gatagtcccg tattctaccg acatagaaca tgcactgcgc gtgcttcaga aggccttcga
    20461 cgaacagaag gcgagcattc cggggattgc ggatggtccc cgcgtattgg gagtccaaga
    20521 gatgaatgaa acgggggttt tcctccgagt gtgggcaact gcgaagccca tggagcagtg
    20581 gtctgtagag aggcagctga agatgatagc gagaaaggct cttgatgatg ccggagtaga
    20641 tcccccagtg acgcaccaac gcataatcat tgatagattt tcggatccag taggctcaca
    20701 ggctgaggaa ggcggggaat gcgaagcatg agctatcggc tgggcgatat agtgagaatg
    20761 aagaaacctc atccctgcgg cagctcaacg tgggagattc tcagggttgg cgcggacttc
    20821 cgcatcaagt gcacgggttg tggccgcgtg gttatgctta cgagagctca gtttatgaag
    20881 gatgtgcggg ggatagctga ttctggcgcg ggaggcgatg tccatgctga atctgggaat
    20941 cgtgggcctg cctaatgtag gcaagtccac tctgtttaac gcgttaaccc gcgggcgagc
    21001 ccaggtagct aactacccgt tcactactgt cgatcccaac atgggaatgg tacaggtccc
    21061 tgacaatcgg cttgatgccg ttgcggccgt tgcgggggcg cagaaagtga ctcctgccgc
    21121 gatccgattt gtggacatag ccgggctggt tcgcggggca agtagaggcg aaggcctcgg
    21181 aaacaagttt ctgtcccata tcagggaagt agatgctgta gtgcacgtgg tgaggtgctt
    21241 ctttgatacc gatgtagcgc acgtctacaa tcgcccggat cctgtgaggg atgtcgagat
    21301 aatagagaca gagctcatac tggcagacct ggaaagcgtg gacaagcgca tcgccaagac
    21361 cgaaccgctt ttgaaaacag gggataagcg gtgccaattc gagatggagg ttctgaccag
    21421 tctcaagaag catctcgaat ctggattgcc ggcacgccag gcgcaactgg acgccgagga
    21481 acgcgaggtc gtggacgaac tcttcttgct tactatgaag cctgtgatat tagtggcgaa
    21541 tttagcggag gaatgtgcgg agcagctggg agagcttttg ccgatgaatt cgatctcagg
    21601 ctcggatgcc agtcgagttg ttcccaatga tgtggcaagt ctccatgctc tcgaagatgg
    21661 ggacggcggc gcaaagggga ctggagaggg ctcgaactca gggattatcg gggagatcga
    21721 gtccattctt gatgcgttga gttcggttgc ccacggggcg ccagttatcc cggtatgtgc
    21781 agagctagaa aggcagctct ctgagctacc tccggatgat gccagcgatt atctggcatc
    21841 cctagggtta gcccattccg cgctggacag tctgattgcc actgggtaca gcatacttga
    21901 ccttatcacc ttctttacga ttaagggccc tgaaactcgg gcatggactg tcgctcgggg
    21961 tacagtggcg ccaaaagctg cgggaaagat acattcagac atggagcgcg ggttcataaa
    22021 agcggaagtg atacaatggg atgagcttgt tggagaagga tcttttgttg cagctcgaga
    22081 gcatggcaag gtaagagtag aggggaaaga ctatgtaatg caggatggag acgtagtgct
    22141 ttttcgtttc aatgtataat cagggtgagc ccggatactt gggatttttc acccgcatag
    22201 tgggttgcgc ctgtctgatg tggatgttat aattcatgtc gcaatccctg ccctgcgtgg
    22261 aacgtagggc cgatagtccg aggggaggtg aagtcagtgc gcgcctacga gagcatgttt
    22321 gttattgctc ctacagtgcc cgaggaagag atcgatgcct tcatagacag gttcgtccaa
    22381 ctaatcgtga acaatggagg cgaagtgact gagatcgaca aaatgggcag gcgccggctg
    22441 gcgtacgaaa tagaggattt cacggagggt tactacgtgg tgctctattt caatgctcag
    22501 cctgatgctg tagcggagct tgagcgggtg tacaagatca cagacaacgt gatacgctct
    22561 atggtcgtcc gaaaggacag ctaattcctt gccgggctgc aaatatcaag gcagggtggt
    22621 gtaatcatga acagggtcgt aatagtgggg cgcttgaccc atgatcccga acttcgattc
    22681 actcagaatg gcatagctgt cactagattt tctttggccg ttgacaggcc ttttacaaac
    22741 cagcagggag aacgtgaaac agatttcctt gatgtggtag tctggcgcag gcaagctgag
    22801 aatgtagcgg catacctgaa aaagggcagc cttgctgccg tggatggccg tgtacaggtt
    22861 cgcagctatg aaacgcccga gggccagaag aggaaggtat gggaaatagt cgctgatagg
    22921 gttgagtttc tcgaccgaag gggcgatacg ggatcacaga tgtcccaggg tgggtatggc
    22981 cgtacacagc cggagctggg tgaagatgaa gacgatgttc caatgcctga agaggctgag
    23041 acattcgggg atcagataga tccggacata cgctgatagc gacagcacca ctgcctacat
    23101 cagtggaggg acaatatagg tgagacgtga aagaggacgc aagtccagaa ggcgagtatg
    23161 ttccttctgc gtcgacaaag ttcaggacat agattacaag gatacagcca ggcttcggag
    23221 atacctcaac gagcgcggga agatcattcc gcgccggatc tctgggaact gcgctaagca
    23281 tcagcgccaa gttacgctcg ccataaagcg ggcaaggcag atggcgttgc tgccgtatac
    23341 tgcagactag tctggtttag atggaaccgt ggctataacg gattggaaaa ctctttggcg
    23401 gcagcagccc ccaaggagtt ttcttgcaca tacgcccatg tgtcctacat tgcattggaa
    23461 ggttgggcgg aaggattttg cctttggtgt cgagaacgaa cgagtccatt gactgcatgg
    23521 cactttaaca cggggaggag tcggaatgcc atatccgaga tacaccgata ttgcctgtgg
    23581 tgttaagaca attgactggc ttgaggcgga gatacttgga agcattgcct cgttgtatcg
    23641 ttctctggcg cgagcggatg aaggcccgcg cgtggccgat ctggctaacg ccatcatcgg
    23701 ttgctatctg ctaggcaaaa ggttaggaat aagttatgct gagctggaac agcaaatctc
    23761 agcgaacatt gatgagaaca tcgagaaggg gcacgaaatc gaaaggtggt atggggactt
    23821 tagcgctttg gcgaatcaca ggaaggggcg ttccacttga gtcaatcacg gaatcaaaac
    23881 ttgcgagcgc ttactgaggg ggctcttctt gccgcactta cagtagtgtt gtatgtggct
    23941 gacgtttata ccagaatact catttacgtg gttccagtgc ccattgcgat tctcgtagta
    24001 aagcatgatc tccgcacagg cctcatggcc gcagcggtct cggctctcgg tgtagggctt
    24061 attttggggc caatagactc tgtgatcgtc tttgtgcgtg tcgcaactct cggtttgacc
    24121 ctcggagtat gcttggcaaa attgcttcca tggcctcgca ctatcgccat taccagtgtc
    24181 gcttcgaccg tgcagattgc catcgatttt atgttggccg cggtagtatc caaactctct
    24241 ccgacagagt tcctgggcag catgatggat gaattcgcgg cggctggcga aagagctgtt
    24301 gaaatctatg aaggtatggg aatgggcgaa acatccatga tgctcgccag gcagtccgtt
    24361 gagctcatga ccgtcttgct taagtcgttt ctgcctgtga ttgtgctgtc agccgcagtg
    24421 ttgggtgcgg tgatagtggc agccgttgct cgctgggtgc ttgtgcgcat gaatgaaaaa
    24481 gtggtgcccg tcccgccctt tgcccgatgg cagcttgggt gggagtggat atggggcttg
    24541 atagcgggga ttctcctttc gcaactcggg caatggatgg gcattgagct catatcgact
    24601 ataggcaaga acgtgactat ggggttcgcc ttgctctata ctgtacaggg agtcgcgatt
    24661 atctggcact tctttacgaa gcgaaatatt ccgaaagccc tggccgttat cgtgataatc
    24721 cttatatacc tgactccgcc gttcaatctt cttgtcccca tagccggagt cctcgataca
    24781 tggcttgatt ttagaaactt ggcggcgcag tagggctgtg cgccaacgta ctttgaaagg
    24841 gggtagcggg cggggaacga aataatgcgc ttcgcccgcg aaaagtcgtg aaagtagtcc
    24901 ttctaaaaga tgtcccgggc ttgggaaaga tggacactgc gctagatgtg gctgaaggat
    24961 atgcccgaaa ctacctgatt ccccggggcc tggctattcc ggcaacctcc aaggagctta
    25021 acagagtaaa caagcttcgc gaggctgaaa agcgcaaggg ggttcgcctt gaggagaagg
    25081 ccagagagga tgcgcgcaaa ttgaaggcac agggggtgac tattcgggct aaggctggcg
    25141 agactggaag actttacggc tcaattacgt caaaagacgt tgccgatgca atccatgagt
    25201 cgttggggat ggagattgat cgcagaaaga tcgagctggc cgactccatc agggaacttg
    25261 gcacgttcac tgcaactgtg aggctttttc ccgggataag cgccgatatt acgatcaatg
    25321 tcatcgatga aggaaaatga agatagttgc ccaaggcgta ccctagggca gagacgaaag
    25381 gggttttgct ttcatgaagt cgctcgttaa gaaattcacc aagatcggca gcaggaacct
    25441 ggcggataag cttcaggacg acgagcaact tgtgcggcag gtgatggccc ttttcgagat
    25501 cctttcagat atttatgggc ctgacaggct agttctgaag gctggaaagc ttgatgcgct
    25561 gagcctcatg cgttctgcgg aacttgacga caggattttg gctctggaac gaatagtatt
    25621 tgaggatccc accattgacc gcaagccatc gcctgaagag tacgaatcgg tcttggccga
    25681 tattgaggaa gagatagcgg atgtgatagc gaggcggcgg gtagaggaca agattgaaaa
    25741 gcaaatatcc gaccgcatgc agcagaggca tgaggattac gtaaatgaga taagattgca
    25801 gatactgaag gaagactccg gacccgatac tgaagatacc ctcaggaagt tggctgaact
    25861 ggaagagctc gaaaagaggc gacttgccaa gtccgcgctg gaaatagtgc gtccgactgc
    25921 catgtcggag attgtggggc aagatcgtgc ggtcagagcc cttcttgcca aggtggcttc
    25981 gccatttcca cagcatgtaa tcttgtatgg ccctccagga gtagggaaga cgaccgctgc
    26041 gcgcctcgcc cttgaggagg ccaagaaggt tgcccttacg ccgtttggca aggaatcgag
    26101 gttcgtggaa gtggacggca cgaccctcag atgggatccc agggaggtca cgaacccgct
    26161 tctcggttca gtgcatgacc cgatatacca gggcgcaaag cgcgacctgg ctgaaggggg
    26221 agttccagag cccaagctcg gtctcgttac ggaggcccac ggcggggtgt tgttcataga
    26281 cgaaattggg gaaatggacc cattgctctt gaacaagttg ctcaaggtca tggaggacaa
    26341 gagggtcaag ttcgagtctt catactacga tccttctgac ccgaatgtgc cgtcatatat
    26401 cagaaggctt tttgaagagg gcgcgccggc agacttcgtg ctgatcgggg ccacaactcg
    26461 tgatccctca gagctcagcc cggctctgcg ttcacggact gcggaggtct tctttgagcc
    26521 acttacccag gtggacatag agaatatagt gagcaatgcc gctgggaagc taggggtgga
    26581 gatggatcct gaggttccgt ctcttgtgag cgactatacc atcgagggaa gaaaggccac
    26641 gagccttttg gtggacgcct atggcatcgc cttgttcaga gagatggata ataggcagga
    26701 cgggaaggcg aacgaagctg catccgggga cggtcctgtc aggataacat tggccgatct
    26761 tgaggagacg atccgtctcg gacggctttc gccgtacgtt aatgttcgcg cctctgatag
    26821 cgccgaggtg gggaagatct ttggactcgg ggtggccgga ttcttgggct cagtgataga
    26881 gttcgaggcc gttgcgttcc cggctcgcga ggagggcaag gggaccatca gattcaacga
    26941 gacggcaggt agcatgacga aggattctgt attcaatgca gcttctgtat tcagatttct
    27001 gactgatagg gaccttgccg atttcgatgt ccatgtgaat gttgtaggcg gcggcgatat
    27061 cgatgggcct tctgccggga cagcggtcct tcttgcaatt cttagttctg tttatgcatg
    27121 cccgatcagg caggatgttg cagttaccgg cgagctttcc attcagggga aggtacgggc
    27181 ggtgggggga atcttcgaga agatatacgg ggcgcgacag gcgggcatca agaaagtgat
    27241 cttgccggcc gagaatgtca aagatgtccc tcccggcatt accggtatcg aggtggtgtc
    27301 ggtttcagat gtcgttgaca cgttcaagca tgtttttgag ggggacgcaa tctttggcga
    27361 attgcgtagg gagtgagtta ctttggcaac tgcggatgta gtgggcgttg atcgtgtgcc
    27421 accgcagagc ctcgaggcgg agcaatcgac attaggttca atgttgctcg ataaagaggc
    27481 gattgctaca gctgccgaaa tccttgtggc tgaggatttc tacagagacg cgcacagagt
    27541 gatattcgat gcccttatct ccctgttcaa caagggtgaa ccggctgact taatcactgt
    27601 tacagaggcg ttgcgccagc gcaatgcttt ggagaacgtg ggcggggctt cgtacatcag
    27661 cacgcttgca aacacggtcc cgacggctgc aaactgtgaa tactatgcca agatcgtcaa
    27721 gaataagtcc attatgcgat ctctggttac agcaggatcg cagatagctg ctttgggcta
    27781 cgaccagacg aacgaggtag cggagtcgct ggacaaggct gagcagctca tattcaggat
    27841 ctcgcaacgt ggggagacgg gcacggtctc tgacatgaag acggtgctga tgagcacttt
    27901 tgatcggatc gagcgccttt acaccacgaa aggggcaatc accggccttt ccacgggatt
    27961 taccgaggtg gacaatatgc tgtcggggct gcagccgtcc gaactgattg tcatcgcagc
    28021 gcgaccatct atgggaaaga cggcgctagc gctgaacata gccgaacatg ttggggccaa
    28081 tgaaggaaag cctgtcctca ttttcagtct cgagatgtct agggagcagc tcgcccagag
    28141 aatgctctgc tcccaggcga cggtggatgg tcagcgactc agacgaggca atctgtcgga
    28201 agcggattgg caacgtctgt cgcatgcgat cggccggctc agcgaggccc ctatattcat
    28261 cgatgatagc ccgacggtta cagcaatgga catacgtgcc agggcgagac ggttaaaggc
    28321 tgagcacggc ctgtcgttga tcatcgtcga ctatctacag ctggttcagg gccacgcgcg
    28381 ggcggagaac cggaaccagg agattgcaga gattacccga tcactgaaga cgctcgcacg
    28441 cgagctggag gtgccagtgg tttccctcgc gcagctcagc agggctgttg aagcgactgc
    28501 cgacaagcgc ccgctattgt cacacctcag agagagcggt gaaatcgagc aatcggcaga
    28561 cgtggttgcg ttcatctacc gtgaagatta ctacaagccc gacacggaac ggaggaatat
    28621 cgcggagata atcatcgcta agcagcgcaa cggaccgacc ggtgttgttg agctggtttg
    28681 gcagagggaa tacacgcggt ttcggaatct ggagaggttt gcccagggcg agtcggcata
    28741 agccgtggtc agatatgccg acccgatctg ccgattcggg aggtcatgaa gtcccgaatc
    28801 cgtcgcgtgc cctcgaggat ttccctctcg cctgcggcat aggatatccg gatgtactgg
    28861 tcgtccttgg ggccatttgg cgaatgaaag gcactcatgg acaggactgc tacgcctgcg
    28921 tcgtccagga gggcatcctg gagctgatct gcgtcagcaa gaccagccat acggcacgct
    28981 tctgtgacat tgggaaaggc atagaatgcc cctaggggca tgtgacatct gaaacctggt
    29041 acacggttga gctcggtcac gacgaggtcc cgccgcttca ggaatttgag gcgcatcttc
    29101 tccacttcat gttggggccc tgtgagggcc tctattccgg cgtattgggt gaatgtggct
    29161 gtgcaggagt gcgcattggc catgaaggtg tcgaacaggg ctgcaagctc cgtgttgacg
    29221 acggcgaaac ccagacgcca gccggtcatg gcataggctt ttgaaaaacc atcaacgagg
    29281 attgtccgct ctgccatgtc tggcagggac gagatgctga cgaagtcgcc atcgtagaca
    29341 agtcgggagt atatctcatc tgacaggacc cacaggtcgt gttcgatggc gatttcggca
    29401 atgcgcgcaa gatcctcccg tgggaggagt gtgccggtcg ggttgtgcgg gaagttcagg
    29461 attatcatgg aagtattcgg ggttaccagg ctcgcaaggc gatcgaggtc caagcgaaag
    29521 ccctggtcgg tatcgatgaa tgggactgga ataaagtttg ctccgatcat acttactatt
    29581 gacgggtagg ccgggtatga ggggacaggt cccaagacgt cggcgccact ttctacgcag
    29641 gacatgatcg cgcagaatat cgcaagtttg gtcccggcgg ccacgactac ttgctcggga
    29701 gatacggata ttccccgggt tctggaaata tagtcggcga tggcactccg gaaggccggc
    29761 actccggacg ggggcgagta gtgtgtcctg ttagcatgga ttgaagagca agctgccgtt
    29821 tttatgtttt caggcgtggt gaaatcgggt tcacctatgt gaaaacctat tatgtcgtga
    29881 ccggaggcaa ccatttcaga aacctttttc agtaccgaaa aggcgcgctc gggttcgagc
    29941 cgctgagccc tttttgcgta gtccattcat tcatcatctc caactacaga gattgtggcg
    30001 cgaagcgccc tgcacacaat gtggactgga gggttattcg catggagaag agtattctgg
    30061 tcactgctgc agtggctgcg cttttctctt tactcagtat tcccgggtat gcgcaggatc
    30121 tcccgcgagg catcgatatt gcccgggtga ctgctgagag gaaacctcat gttctgttgg
    30181 tccatggctg gctggctacg tccctggaca tgagcgtgct caaatacaag ctggaacagg
    30241 ccgggtttga atgcgaaacc atcaatttct cgtatcttgg ggcggtgagc tccatagccg
    30301 actacgccgt ggagctggcg agaaaggtgg aagtcgattg tgcgggcaag gctgatgtgg
    30361 caatcgtggc tcatagcatg ggcggattgg ttaccagata ctacttggat aacctgaggc
    30421 agacggccca ggtgagtctg gtcatcacca tcggaactcc gcaccatggg acactgagtg
    30481 gcaagatcgc tcccaccgca agtagatgcg ctagggagat ggctccggga agtgagcttt
    30541 tgcggctcct caatgctcaa gagggggatt gcccgggtgt tgaattccat gcgatctgga
    30601 ctcctacgga tggggtggtt attccggcgg agaatgctat aatgtgcggc gcgaagaact
    30661 atcaggtcac gggctttgga atggctcata tgacgatgct tgcgagcgat acggtctttg
    30721 agattgtcca tgccatactg cgggggcagg atgtggacgt atccggtcct cagctgagag
    30781 atcatacaga ggctgaaaag cctgtggttt ttccatggca gtagcatgaa attgtgccag
    30841 accctgtggc gacgtccagg ctattctcgg tgagtgcctt gaaagatcct ctgtgtacgt
    30901 gatataatgg cgaagctaaa gatgttgagt gggccactag ctcaggcggt agagcaccgg
    30961 acttttaatc cgggtgtcgc gggttcgagt cccgcgtggc tcaccatttt tatacccttt
    31021 tagctg
//
LOCUS       DAOSYL010000026        29555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1300_length_29555_cov_7.697593, whole genome shotgun sequence.
ACCESSION   DAOSYL010000026 DAOSYL010000000
VERSION     DAOSYL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 29555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29555
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1300_length_29555_cov_7.697593"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLU15_05925"
     CDS             <1..480
                     /locus_tag="PLU15_05925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO30494.1"
                     /translation="NAVEEAFKMMTYGVYIIGTAAAGKINGMTAAWVTRVSVDPPLLL
                     VAISKGHYTTELIRASRVFSVNLVSPQQAELAAKCGSVSGRICNKLVDEEIEYGETGA
                     PILTEAAAWLDCEEIDEFECGDHICFVGRVVGAGKRSEQALMYRKGSMQPVPAQNDA"
     gene            complement(608..1291)
                     /locus_tag="PLU15_05930"
     CDS             complement(608..1291)
                     /locus_tag="PLU15_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30495.1"
                     /translation="MASITAADVVSSIKAIAGTIDFASLGPYLMLNEDYLRHYLSAIL
                     NWDFRLLNLTGAIQPVQLHPEWPTYKEQTHLDYGRYGQLTNGEGETEYWPKEHGSAGF
                     IDFAIGDYESPEIGIELTMEYGWTHEEIVYDFIKLMDARNPFKVAISYNVLLRPAGFV
                     DRVDEPQHLLDNMNRAIADASARLGDGICDDSRQLVFIFTETDEDARRRHWHYDQHLQ
                     TFVKGLPNT"
     gene            complement(1462..2046)
                     /locus_tag="PLU15_05935"
     CDS             complement(1462..2046)
                     /locus_tag="PLU15_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30496.1"
                     /translation="MRKRIVTLLLFAVGLSLLTAGCPDTGIFQKKPAASYSKEDTFGP
                     YRITVSSVAKAKDTTAPEEGKITVARTGMKTVSTVFETDPVYTSWVTDLDSNDKFEVL
                     VWSVSPDEEKRGALHVYEWNEPELREQPFPDLTEAQKAGYRGHDDYSVDNNKVVRSFP
                     VFSTEGGADAQSSHKRALWYSYHNGEWKLEQESE"
     gene            complement(2604..3860)
                     /gene="larC"
                     /locus_tag="PLU15_05940"
     CDS             complement(2604..3860)
                     /gene="larC"
                     /locus_tag="PLU15_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOO30497.1"
                     /translation="MMIAYFDCYSGISGDMILGALIDAGLDPDDLLSELSRLEQGGLE
                     HFDLAVSKVLRKGIGGTHVEVITAEAHQERDREHSHDHHHGHDHEHDHDTLFRNLSAI
                     EKLLDGSGLDAEVRETSRAIFRRLAEAEALVHGIPVDRVHFHEVGAIDTVVDVVGAVA
                     GLRIMGVERVFASPIHVGTGLVRCAHGTLPVPAPATAELLRNVPVYATGVRGELATPT
                     GAAIITTLAESFGPIPEISIERIGYGAGRHDFEIPNLLRVLIGKTMQHYSTEDGDAAC
                     DLVTVECNIDDMNPEFYEHVMDLLLKRGALDAFVTPVLMKKGRPGHLITVLTPRAALD
                     IVTTTLFEETTTLGVRIQEVSRRTAQREWINVSTEYGVARVKVARFGDRLVNVAPEYE
                     DCRKLAKEYGVPLKAVYDEVKALARE"
     gene            complement(3871..4644)
                     /gene="larB"
                     /locus_tag="PLU15_05945"
     CDS             complement(3871..4644)
                     /gene="larB"
                     /locus_tag="PLU15_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOO30498.1"
                     /translation="MDPKSILLLLESVRDGDIDPGEALEELRMLPYEDLGFAKVDHHR
                     NLRTGFPEVIYSSGKTPEQVARIAQAILDRGASVFLATRADTDTFDAVRQVAPDAVYH
                     ETAHIISVQRDLPTPTEGMVLVAAAGTSDMPVAEEAAVTAKIMGNKVERLYDVGVAGI
                     HRLLDKVDLLRSARVLIVVAGMEGALASIIAGLVDKPVIGVPTSVGYGASFGGLAPLL
                     TMLNSCAAGVSVVNIDNGFGAGYLASIINRLGEDNPDPI"
     gene            complement(4679..5593)
                     /gene="larE"
                     /locus_tag="PLU15_05950"
     CDS             complement(4679..5593)
                     /gene="larE"
                     /locus_tag="PLU15_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOO30499.1"
                     /translation="MPEHAPKGHCPGNCVSHPSHRDYSPHCSSDSGNALEAKLDNLES
                     ILAEMGSALVAFSGGADSAFLLATAHMVLRGKVVAATISSPVHSRTELDQARRFADGL
                     GIEHEVIHLDLLGIPRFASNPSDRCHTCKRAVFEALLSLAQQRGIPYVIDGSNADDAS
                     DYRPGMAALTELGIESPLMGAGLTKQEVRTLSRRMHLETANKPASPCLATRIPYGHTI
                     TQAKLDQVQLAEDFIRTLLPKDSDHIQLRVRHHGDTARVEVTPELMHCIVDHARSISE
                     FFRQLGFTYVALDLGGYSQGNLNRLLEP"
     gene            complement(5704..6036)
                     /locus_tag="PLU15_05955"
     CDS             complement(5704..6036)
                     /locus_tag="PLU15_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOO30500.1"
                     /translation="MRVSEEYDLIVTIINKGWADEVVEASRRAGAEGGTILYGRGTGV
                     HEQKTFFGILIEPEKEVVLTLIPRSRTDAVMEAVVAAGQLEKPGTGIAFVLDAGKVAG
                     IVHRKDAE"
     gene            complement(6003..6773)
                     /locus_tag="PLU15_05960"
     CDS             complement(6003..6773)
                     /locus_tag="PLU15_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOO30501.1"
                     /translation="MGEIIVFQGFPAVMAEVGMTLIPLAILFFIFQTFFLKLPRTFMR
                     KVVVGMISTFIGLSIFLQGVNIAFLPIGKMLGAKIGGLSNPIYALPVGFILGLLVVLA
                     EPSARVLSGEVEKASAGSIRESVLLRTMASGVAVLVGLGYYFIARGIALTWIIIPGYI
                     IALILLKWVDPAFAAIAFDSGAVATGPLTVTFLMSLGLGIAAEKLGGPTMNAGFGLVC
                     LIALASTLAVEGMSFIFKRNSKKEDGRKCACQKNTTSS"
     gene            complement(6773..7465)
                     /locus_tag="PLU15_05965"
     CDS             complement(6773..7465)
                     /locus_tag="PLU15_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOO30502.1"
                     /translation="MSTPLQEAIAEVVAAVAPLTIAVAVLSIIFLGFSWTNLIQLLIG
                     AFFTTVGLFLFLRGTRIALLPMGEMIGAYLPRHGSMALLVATSFIFTFSVTIADPSVH
                     VLVSQVASFPDAGINSDFLAIFISAGMGLLVTVAILRIVLNVPIKYIFLGAYSTAIIL
                     SFFTKPELVPFFFDSGSVSTGPMAVPCIISLGLGTASVLQRKQSLADGFGLVGLATLG
                     PILVLMIWGVFI"
     gene            complement(7624..8250)
                     /locus_tag="PLU15_05970"
     CDS             complement(7624..8250)
                     /locus_tag="PLU15_05970"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO30503.1"
                     /translation="MDPKWLEWGLRLQAVAQNGLSYAHGPYDIERYELIRSIATEMMS
                     EQAHADPEIVRDIYTLETGYATPKIDVRGAIFADSSILLVKEKADGCWTLPGGWAEIG
                     ESPSEAVEREVFEESGYRVRASRLLALYDRGKHPHPPAPYHIYKLFFECEIIGEPSTP
                     SPDGIETDAVEFFPSDRIPNLSIPRVTPDEIRRLFQLHADPQAPTDFD"
     assembly_gap    8446..8545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8546..11827
                     /locus_tag="PLU15_05975"
     CDS             <8546..11827
                     /locus_tag="PLU15_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOO30504.1"
                     /translation="VDHLIWRTEALLGRGVWLAAVTGTNFVNLDRQFFSLLNPKVKTN
                     LLACVNRGSEVYGFDSEGRPVRLWAREATCAENTMMDQAVTMAQRVLKEQYGLATRIV
                     SDRMNRRKLDIMPFPEWEDPPKERIGELLDETQGRLRIAGLEGIESVIDLVGRCSEEV
                     GLDGRITSDVKHVELGLTDKADSVDYLVEEVAKTHGIEVSEILFIGDEFGSIGGCAGS
                     DSKMITPQTESASFISVGQEPEGVPPAVCHMGGGIESFAKILEAQIEMWRDDEENMFG
                     RDRLTDSLLLLPAEDNWVAYETCCDAETAASYETLFALTNGYLSVRGAHEDGLYRGNA
                     GSFVAGVFDKSSGPYDVTELVVIQDPLPVEIVVDGERVGNEGVRDYRRELDMRGGLLR
                     RFYRWSDGAGRVVRVESERFVSSVDEHLLCARLSVTMERGSGRVLLRSRLDGNVSNSG
                     AVHLKVDSVAARGDGTAEVCTVTMESQAGIAMACAQQASFVEAPGKARGEPDEALGTP
                     VYPRPFSGESAAGHDFEVRLAKGDSIEICKFVAVHTSRDVAWRACKAPPAMDQLRTAA
                     QASAEEAASRGFSGCRLEHLEEWRRQWERCDIRVDGPDRDQMAVRFAVYHLASMGPQS
                     DDRVSIAAKGIHGEGYKGHVFWDTEIFILPFYVAQFPDVARRLLMYRYHTLPGARRKA
                     REKGYRGAMYAWESADSGDETTPKWSAPHPETGERVRIWCGELEDHITADVAYAVWNY
                     CRWTGDRGFMKEHGMEMIVEAARFWASRAQWNEEKSRYEILGVIGPDEFHEHVNNNAF
                     TNYMARWTLLAAFRLAEDFPIKWEALLRRLGISEAELDEMRRVTNLLYLPLPDPVSGV
                     IEQSEGYHGLEDVDLAELKAIAEDSGKSVEENFGRERLIGSKLIKQADVVMLGRLLPG
                     LFGEDIWKANWDYYEPRTTHLSSLSASVHSTFASHLGLPEEAYAYFERCISIELEDEK
                     RNLEDGIHAANLGGIWQAVVRGFAGIQLLDPASQDGSCCGCGKEADPVISINPRLPKT
                     WDRLEIPLACRGRHLRAVITPYRAAFANETQDAEADGGGSVIIECVGQRKRIAPGQST
                     EFVLD"
     gene            complement(12495..13895)
                     /locus_tag="PLU15_05980"
     CDS             complement(12495..13895)
                     /locus_tag="PLU15_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30505.1"
                     /translation="MQSRAVDRRAAGARSNQPSKAMARRRWARTASIYVLLMFFSIFF
                     MGPLVFAFLSSLKDNPTEWPPTLSSPQLSPVNWGAAYRLGKAGAGDGFFGAFAPGAIV
                     PFSATYEVDEGQDPEPPIVVVPRRVPGAGAGAVRLGPFAADEAVVSKVREVSRGKVDG
                     RVRVTYEWEIRHTGELTYDRLPMDVEVPLYQKFVSATLAPNRLERLGRVQSWNNVTSG
                     IIPYVFYNYHRVFRENYSRTTGKSLFLLWIRNSFLLSAIRVVTTLLIASMAGYALARL
                     DFAGRNSLFFLTLFSMMIPGQVTFISNYLVLRDGIFGLSKLFGVDTLINTWPGYIIST
                     MVNGSAVFIMKQFFESLPHDLEESARIDGASTFQTYFKIMLPLAQPSLGALAILTFQG
                     TWNEFFWPLMILTSPPDKYTLTIGLLNFRSTYAVAFDWGPMLAGTIISALPIIVLFIV
                     FQSYFVEGISFTGIKG"
     gene            complement(13968..15080)
                     /locus_tag="PLU15_05985"
     CDS             complement(13968..15080)
                     /locus_tag="PLU15_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30506.1"
                     /translation="MKPKQSSAVLEKASPYIMLAPYIFFIAIFFGYAFLRTLYFSFTD
                     YNMFSKPVWVGLKNYADLFRNVQFGRAFKNSVTFSIIVTFFQTIFALILAIVLNTKIR
                     GIKFFRALYYMPSVTSSVVITLIFMWLFQRNGIFNYLTTSFLERKAIIGAFFASLVVL
                     QVLMVITERTRKQPCSWFEPSFILASALLSLVVVWLLQFAGVVSPNPGVEPVRTIWLN
                     TRQTIPEWAGKLGIPRPLVAIMILNTWTTAPTMMLLFLAGLQDIPSELYEAAAVDGAT
                     GFAAFRHITLPGLKHVTYLVVTMGLIGTLQMFDQVAIIGDQAPLESVITLAYFVYHNA
                     FPGSAVPRIGAASAAAMVLATFTFILTWIQRKLSRA"
     gene            complement(15263..16516)
                     /locus_tag="PLU15_05990"
     CDS             complement(15263..16516)
                     /locus_tag="PLU15_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30507.1"
                     /translation="MKIRRVLCVAILAAMLACATASQAANVKIRVAGFGGTDTAIVEE
                     LIARFVQPNLKGITVAYEPIPDAYDKWIVNALSAGTGPDLFYVDIFWAQGLFSSGAVE
                     PLDPYLAKSNILQGKDIIPALLNAFTYKGKVYGIPKDFNTLALIYNKDMFDIAKVPYP
                     DKNDDWNTFTAKLQKVQAAFPGVYGVCLSPEFARMGAFAYATGFRSFDKNGKTDILSK
                     EFRDAFNWYTGLAAKGIGVMPADIGQGWGGGALAVEKVACCFEGAWIVGFLRDESPNL
                     NYGATLLPKYPGTGQRGNLTFTVSWSMNAASKHKDEAFKVLELLTSPEAQQWVLERGL
                     ALPSRRALVNNPYFKKTDKEAVANQVVFEGAADGYVMPFEFGQYGGDWMMPINDALVS
                     VMSGQQSADAALQEAQKRLNELIGK"
     gene            complement(16714..17778)
                     /locus_tag="PLU15_05995"
     CDS             complement(16714..17778)
                     /locus_tag="PLU15_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO30508.1"
                     /translation="MSRTTLKDIAEQAGVSVTAVSRALSGYPDISEETRSRILRIACE
                     LNYQPNIAARTLVTQKSGVMGLFVLGREKGEGFSHPFSGQVINGFLDELTEEGYDMMV
                     FKANRPPDSTNSSYLDLCRRRMVEGAFLMGLRTDDPWLADLTTSTIPIVTIDMPLAGD
                     MTFSVGCDNLSAVHSAIRHLADLGHRRIGIVNGYKQASVSLERLQGYRNALDDLEIPF
                     DPSLVAEADFTAKGGKKAFLSMIDRSPDMTAVFAASDLMAMGVLEGARERGLTVPDDL
                     SVVGFDNIDMAAMSSPPLTTLDQPRYELGQTAADMLISACNGETPPSRVLLKANLIVR
                     SSTAEPNKRRPAREVVQKAH"
     gene            complement(18690..20033)
                     /locus_tag="PLU15_06000"
     CDS             complement(18690..20033)
                     /locus_tag="PLU15_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOO30509.1"
                     /translation="MEQRKTRVGLIFFPAFDWAITPDHPEREERLLYTRDQIIEEGLL
                     DVPGVEEYRPAIATDTDILRAHVCVPTVNRVITESHRIAAGGAMLAADLVLSGEVDRA
                     FALVRPPGHHAMRIVHGSRGFCNINNEAIMVEHIRRTHGPKMRIAIVDTDAHHADGTQ
                     DIYYNDPGVLHICLHQDGRTLYPGTGFIDERGGPRAYGMTVNVPLPPGTGDEGILYVL
                     ENLVLPILKDFEPDVIVNAAGQDNHYTDPLTNMNFTAFGYARLTDLLAPDIVVLEGGY
                     SIEGALPYVNVGILLALAGLDYSAVREPDWDPEIARQPQRVTEEIHRLTGKLQEMWAD
                     REEADIGAIFGKGEYFERGRRIYYDTDNIVEQQREQIRLCPSCSGWRAVFSHALHAST
                     GRTAQIAAMLVPWQACPDCRKAAYSQFELAQKSRAFDRVYLQDPDDDNFLSSRGT"
     gene            complement(20036..21781)
                     /locus_tag="PLU15_06005"
     CDS             complement(20036..21781)
                     /locus_tag="PLU15_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOO30510.1"
                     /translation="MRNRCDIVGIDVGGTNTDAVLVRDEDVIAAAKAPTNPDNLLEGA
                     QIAYRQVVEAHDGTSPIELHLSTTLSTNAIIEGQGDPTVVLATPGPGMSFDDMGLGFP
                     VHALSGYIDHRGREVAPVDVSAAMSTLKAACDDGAKALAIVGKFSHRNPAHELQIEQE
                     VIAEFPQYSPATLGHRLSGRPNFPRRLATAYLNASIARQQSLFAEMVQNLITGDEVER
                     VFILKADGGTMRLKESMIRPIETILSGPAASTMGAKALTRHPGANAVIVDIGGTTTDI
                     SVQVAGEPIYDRYGAEISGHKTLVPALFSRSIGLGGDSSIHVRLVNGAPTFYVGPRRA
                     GVPVVRGGSKPTPTDAAVAEGLSSLGSRELATRAMAQLGAPYGMDASSVAKAVLDAFT
                     RQLADEIEAVYAHLESIPAYTVSEILAPPDIRPAMLIGLGAPAPVFIPPAADRLGISW
                     EILPYYEEANAVGAAASQPTAAITLHADTMLGVLTVPEADHVERIKRPITFDIKQARS
                     EAIAWACRFAEEIGVEASQGVSIVEEESFNVVRGFYTAGHIYSIKAQVRPQVRRIKAA
                     ETSGVEGPDQSKEDV"
     gene            21993..22667
                     /locus_tag="PLU15_06010"
     CDS             21993..22667
                     /locus_tag="PLU15_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOO30511.1"
                     /translation="MQKLYSGKTKDVYLADDGNVLLYFKDSVTGVGDKIDSGANEVIG
                     EVAGKGHSSFSLTLMFFDLLHKAGIPTHFISVGPEDNTMLVKRARSLNLEVICRKKAW
                     GSFVRRYGAYVCEGQPLPSLVEFTLKDDDRGDPLITEDALVALNIASRQDIDYMKETA
                     RRATDLISGYLADRGLELIDIKYEFGVIDGKTMIIDEVSGDSMRVVKDGKVLLQNELY
                     EALLGR"
     gene            complement(22852..23061)
                     /locus_tag="PLU15_06015"
     CDS             complement(22852..23061)
                     /locus_tag="PLU15_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30512.1"
                     /translation="MGSSDTAKSLFFVRVIGGWITAVGSFIVAIAVTTEFSQMYPPCA
                     PQVPVPCANSKEGALKDVANAANWQ"
     gene            complement(23433..23621)
                     /locus_tag="PLU15_06020"
     CDS             complement(23433..23621)
                     /locus_tag="PLU15_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30513.1"
                     /translation="MKSNSCKSTLALLRVIGGWILAVGSFIVAVAVTLEFFQDDCSKH
                     DPDPSGRYVIEVKRAGTD"
     gene            23957..24403
                     /locus_tag="PLU15_06025"
     CDS             23957..24403
                     /locus_tag="PLU15_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO30514.1"
                     /translation="MRQTFLAPAVRDSGRLGWDDMDIAISPNNPVPMYQQIVDQVRAQ
                     ILSGELKPGDPLPSIRKLAADLLASVITTKRAYADLEAEGLIVTRPGLGTFVADLSLE
                     DIERLKAREVIGHLANAVEEGRSLGLEDSEIAELLQNVLDGRVNYG"
     gene            24396..25127
                     /locus_tag="PLU15_06030"
     CDS             24396..25127
                     /locus_tag="PLU15_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30515.1"
                     /translation="MDDAVLQLCKISKAYPGFELKDVSFELPRGYIMGLVGPNGAGKT
                     TIIRIMMNLARADSGQAAIFGLDNVAHETEVKRRIGYVPENHNFYDDMTAGWMARFVR
                     RYYPTWDQNYFDALAARFEIDLGKKVGALSKGNRAKLSLALAMAHRPELLILDEPTSG
                     LDPVVRRELLAELLDFMGDGERSVLFSTHITSDLDRVADYITIIVDGRIHDSDEKDAL
                     LEKARKDEPTASIEDIVILAARGEK"
     gene            25129..25797
                     /locus_tag="PLU15_06035"
     CDS             25129..25797
                     /locus_tag="PLU15_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOO30516.1"
                     /translation="MTNLIWKDLYVNRSMIFIAIGLFGGLFVFGALTGSEEIAAYSLG
                     IMNTFSPMMVPFCALNSAANEEKNRTLALLRSMPISPRTIVASKFISSLLAGILWSVI
                     NMVAASWGTRLFPGAGGEFPPSSLTGLYMMALTLPIAAVTLIAFFKWNSSAARNAIMA
                     LWVALYVIPTAFRPAGMSMTEFYLKIMSPTPSMISVVLVACLGVYVVGMLVAQAVFAR
                     RELA"
     gene            25872..26546
                     /locus_tag="PLU15_06040"
     CDS             25872..26546
                     /locus_tag="PLU15_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOO30517.1"
                     /translation="MISLVWKDLYTSRLMLLLPIGLFGGLLVFGALTGNDEIASLYFS
                     SLANTYSMLMVPGCAFANASNEEKNRTLSLLRSMPISPQTIVASKFLAPLFAGVLWSL
                     ISVAALLLGARLFPKAAGIALRSTPFLGPYLVALTLPTAAMILIVSFAWNANVAGIAF
                     VVLSAALYMISAIFRPAGMSIMGFYLKIMSPTPSMVSIAFAVSLAIYFLGMLVAQVIF
                     VHRELG"
     gene            26575..28725
                     /locus_tag="PLU15_06045"
     CDS             26575..28725
                     /locus_tag="PLU15_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOO30518.1"
                     /translation="MATVYEHIVNPGSIVVVGASDDVSKPGGRVAKNIVDHGYAGNLW
                     AVNPKHDDVLGLATFKSVGDLPGTPELAIIAIPARGVRGVLEELAAVGTRAVIILSAG
                     FGEVSEEGKREELALVEIAERHGMTLIGPNCMGVLTPCYAGVFAGPIPKLEPGCVDII
                     SGSGATADFLAELAIERGLLLSSVFTVGNAAQVGVEDILGMMDEHFGSGSAKVKALYM
                     ESVKKPQKLLRYARSLISKGCSIVGIKAGATGAGKRAAASHTGAMASDDTAVEALFEK
                     AGIIRVSSRSELLDVASVLLMAGPIDGRNVCVLTDAGGPGVLLADELARQGLKVPLLR
                     EATQKRLGEVLWPGSSALNPVDCLSTRTPSQIQQAVDVISEEERDRMDVMPLIMGDPG
                     MGGVAEMYDVIARAMDESPIPVIPVFPSPITSALALEDFKARGKSYFVDEVQLGRSLG
                     RVINRPRLDEPVETLPGYCREAISRALQGDEAHGSAHADNTRTALAPQTAAALLRAAG
                     IPIPGQAVAVGVEDLDGICSDLGFPVVMKVVGPLHKTDVGGVALNVASIEDARDTWRR
                     LMSIDGATGVLVQPMIEGTELIMGATREEGFGALVMFGLGGVFTEVLKDVRFALAPLG
                     MEKCKRMIDSIRGRRLLDGARGGRAVNVGAAADILARVGLLVSDFPEIAELDLNPVKG
                     YGSELYAIDARIIVERRPDEGGALARLRSRHHAR"
     gene            28909..29157
                     /locus_tag="PLU15_06050"
     CDS             28909..29157
                     /locus_tag="PLU15_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30519.1"
                     /translation="MTIGFAGGEIVGIEGPNASEKSTLLRTMCGIHLPISGEVLVYGK
                     RPDIFDSDFKRRVGYVSSRRTSSFALHCEFYGMDKHEW"
     assembly_gap    29196..29205
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6335 a         7624 c         8720 g         6766 t
ORIGIN      
        1 aacgctgttg aagaagcgtt taagatgatg acttatggtg tgtacataat aggcacggcg
       61 gcagcgggga agatcaatgg catgacggct gcatgggtca cgcgagtatc ggtcgatccc
      121 ccattgctgc tcgtagcaat ttcaaagggc cattacacta cagagctgat cagagcatcg
      181 agggttttct cagtaaactt ggtcagccct cagcaggccg aacttgccgc aaagtgtggt
      241 tcagtctctg gccggatatg caacaagctt gttgacgaag agatagagta cggcgaaacg
      301 ggcgctccga tactgaccga agctgccgcg tggctcgatt gcgaggaaat tgatgagttc
      361 gaatgcggag accacatatg cttcgtgggc agagttgtcg gcgccggcaa gagatcggag
      421 caggcgctga tgtacaggaa agggagtatg cagccggttc ctgcccaaaa cgatgcttga
      481 ggaaagattg atcggggaat aggccttaag aacaggtatg agccggaagg gccgatggtc
      541 cgccgcggtc aggcgacggg atacggtcac ttccggcttt ccgttgcgtt gtcgaccgat
      601 ctgctttcta cgtattcggc agacccttaa cgaaggtctg caggtgttga tcgtaatgcc
      661 aatggcgcct tcgggcatct tcgtctgttt cggtgaatat gaatacgagt tgtcgtgaat
      721 cgtcgcaaat tccgtctccg agccttgctg atgcatcagc gatcgctctg ttcatgttgt
      781 cgagcagatg ctgaggttcg tccaccctgt ccacgaaacc ggcgggacgc aggagcacgt
      841 tgtaggagat cgcaacctta aatgggtttc tcgcgtccat gagcttgatg aagtcgtaca
      901 cgatttcttc atgggtccac ccatactcca tcgtcagctc tatccctatc tcgggactct
      961 cgtagtctcc tatcgcgaaa tcaatgaacc ctgctgatcc atgttctttc ggccagtatt
     1021 ctgtttcgcc ttcgccattt gtcagctgcc cgtaccggcc atagtccaag tgtgtctgtt
     1081 ccttgtatgt gggccattca ggatggagtt gcactggttg aatagcccct gtcaggttga
     1141 gcaatctgaa atcccaattg agtatcgcag aaaggtaatg gcgaagatag tcctcattga
     1201 gcatgagata cggcccgagc gacgcgaagt cgatggttcc cgctattgcc ttgatgctgc
     1261 tgactacgtc ggccgcggtt atcgatgcca ttcatatcgc cccctggcag tagtctaaaa
     1321 ctcctatgca tggatgggcg gtggcgatgg gcatgcatga gcctgaacgc aaccgccatg
     1381 ttcctgcttc gagttcttgt tggttcttcg ccatcgagtt ccatatacct tcgtccgcca
     1441 gggtgagcag accgcgcgat cctactctga ttcctgttcg agcttccact ctccgttatg
     1501 gtagctgtac cacaaagccc gcttgtgcga agattgggca tctgctcccc cttcggtcga
     1561 gaaaacgggg aaggatctga cgaccttgtt gttgtcaact gaatagtcgt cgtgccctct
     1621 gtacccagcc ttctgtgctt cggtcagatc ggggaatggc tgctctctca attcgggctc
     1681 gttccattcg tacacgtgca aggccccgcg cttctcttca tccggggaca cactccatac
     1741 cagcacttcg aacttgtcat tggagtcgag atccgtaacc cacgatgtat aaacagggtc
     1801 agtctcgaag accgtcgaga cggtcttcat tccagtgcgt gccacagtta tcttgccttc
     1861 ttcaggagca gtcgtatcct ttgccttggc gactgaggac acagtaatcc tgtacgggcc
     1921 aaatgtatcc tccttggaat aactcgcagc aggctttttc tgaaaaatgc ctgtatcggg
     1981 gcagcctgca gtaagaaggc taagacctac agcgaatagt agaagcgtca caatccgttt
     2041 tctcatttct gttcctccct tatgcatttg gaatagtgca ctcgcgggca cagctattat
     2101 tcccaaaagt ggcacttgca taaccacagt gaagaaattg tgagggaggg ggagggaagc
     2161 catgggatca ggatgagctg gcggttgatt gggagcatga agctgacgtt acgtgtgtgg
     2221 gttttgccat gatgtcatct tggtcgaaac ccgatcgtcc ctcgggcagc agaaccatgt
     2281 ttgaactctg aagctggtgc cgcttcgccg agctccagac tatttctgaa agaagattgc
     2341 catgagttcg catttgggtt tcaccttgca cttggggtct gtttatgcac cgggtattgc
     2401 cataaggagg cagactccgt tttgatgctg tgggacatgg ctaaaacttg aacacttgca
     2461 attgaaaaat atgtcaaagc tagacatgcc cccaaacgtt taccccggag cccggcagag
     2521 agaagctggc tatatgatga tggcgctctg gtcgcaggtt tgcgcggata agcatctgca
     2581 cagagacaac agacgaggac tatctattcg cgcgccagcg ccttgacttc atcatacacc
     2641 gccttcagcg gaaccccgta ctccttagca agcttcctgc aatcctcata ctccggcgcg
     2701 acattgacga gtctatcgcc gaatcgcgcc actttcaccc gtgcgactcc atactccgtg
     2761 gagacattga tccattcgcg ctgggccgtc cgccgtgaga cttcttggat gcgcacgccc
     2821 agggtcgtag tctcctcgaa aagcgtagtt gtcacaatat ccagtgcagc gcggggagtg
     2881 agcaccgtaa tgagatgtcc ggggcgtcct ttcttcatca gcacgggggt tacgaacgcg
     2941 tcaagggcgc cccttttcag cagaagatcc atcacgtgct cgtaaaactc cgggttcatg
     3001 tcatcgatgt tgcattccac agtgaccagg tcgcatgctg cgtctccatc ctcagtggaa
     3061 taatgctgca tcgtcttgcc gatgagcacc cgaagcaggt tgggaatctc aaaatcgtgc
     3121 cgcccggccc cgtatcctat tcgctctatg cttatctcag gtatgggccc gaaggattcc
     3181 gcgagggtag tgatgattgc cgcgccggtt ggcgtggcaa gctctcctcg aacgccggtg
     3241 gcgtacaccg gaacgttgcg gagcagctcg gcggtggcag gcgccggcac gggcagggtg
     3301 ccgtgggcgc acctcacgag gcctgtccct acatggatgg gcgatgcgaa taccctctcc
     3361 actcccataa ttcgcaggcc tgcaacagcg cctaccacgt caacaaccgt atctatcgcg
     3421 cctacctcgt ggaaatgcac gcgatcgacg ggaattccgt gaactaatgc ttcggcttct
     3481 gcaaggcgcc ggaagatggc gcgactcgtt tcgcgaacct ccgcatccag gccgctccca
     3541 tcgagcagct tttctatggc cgagaggttt ctgaataaag tgtcgtggtc atgctcgtga
     3601 tcatggccgt gatgatggtc atgagaatgc tcgcggtcgc gctcttggtg ggcttcggcc
     3661 gttatgacct cgacgtgtgt gccgccgatg cctttccgca gcactttgct gactgcgagg
     3721 tcgaagtgtt cgagcccgcc ctgctcgagc cgggaaagct ctgaaaggag atcatccggg
     3781 tcaagccctg catcgatgag ggcgcccagg atcatgtcgc cgctgatgcc gctataacag
     3841 tcgaaatagg ctatcatcag tgaatgcctc ctaaatgggg tcgggattat cctcgcccaa
     3901 ccggttgatt atcgacgcga ggtatcctgc gccgaaaccg ttgtcgatgt tgaccactga
     3961 cactccggca gcgcagctgt tcaacatggt cagaagggga gccagtccgc cgaaactggc
     4021 gccatagccc acgctggtgg ggactccgat gactggcttg tctacaaggc cggcaattat
     4081 gctggcgaga gcgccttcca ttcctgccac aactatgagc acccgtgctg accggagcag
     4141 atcgacttta tcgagcaggc ggtgaatgcc tgccacaccg acgtcataca ggcgttccac
     4201 cttgttaccc atgatctttg cagttaccgc cgcctcttcc gcaacaggca tgtcggaagt
     4261 ccccgcggcc gccacgagca ccatcccttc ggtgggggtc ggcagatccc gctggacgga
     4321 tatgatgtgc gctgtttcat ggtagactgc gtcaggtgct acttggcgga cggcatcgaa
     4381 ggtgtctgtg tctgcgcgag ttgccaagaa tactgaggct ccccgatcca agatggcctg
     4441 ggctatccgc gctacctgct caggtgtttt gccactcgag tatattacct ccgggaaccc
     4501 ggttcgaaga ttgcggtggt gatcgacctt ggcgaaaccg aggtcttcgt atggcaacat
     4561 gcggagctcc tccagggctt cgccggggtc tatgtcgccg tcccttaccg attcgaggag
     4621 aagcaatatg ctcttgggat ccatcgtgtg catcgcccct ttcaacgctt cgccatagtc
     4681 agggctcgag tagccggttg aggttgccct gggaatagcc gcccagatcg agggcgacgt
     4741 atgtgaaacc cagctgtctg aagaattccg agatggatct ggcgtgatcc acgatgcagt
     4801 gcatgagttc gggtgtgacc tccacccgcg ccgtgtcgcc atggtggcgc acgcgaagct
     4861 gtatatggtc agaatctttg ggcaatagcg tccggatgaa atcttcggcc aactgaacct
     4921 ggtcgagctt ggcctgggtg atcgtgtgac cgtagggtat ccgtgtagcc aagcacgggc
     4981 tggctggctt gttcgcggtc tcaagatgca tccttcggga gagtgtgcgg acttcctgct
     5041 tagtcaaccc tgcgcccatc agcggacttt cgatccccag ctctgtaagg gccgccatgc
     5101 cggggcggta gtccgatgca tcatcagcat tggatccgtc aatcacatag ggtatcccgc
     5161 gttgttgggc cagggagagc agggcttcaa agacggcgcg cttgcacgta tggcatcggt
     5221 cggaagggtt gctcgcaaac ctgggaatgc ctagaagatc gaggtggatg acttcgtgtt
     5281 caatgccgag cccgtctgca aatcgccgag cttgatccag ttctgttctg ctgtgaacag
     5341 gggaggatat cgttgcggca accacttttc ctctaagcac catgtgggca gttgcgagca
     5401 ggaacgcgct gtcggccccg ccggagaagg ccacgagcgc gctccccatc tcagcgagta
     5461 tgctttctaa gttgtccagc ttggcctcga gggcattgcc agaatcagag ctgcaatggg
     5521 ggctgtagtc gcgatgcgag ggatgggaaa cgcaattgcc cgggcagtgc cctttcggcg
     5581 catgttcagg catctgtcag tgcctccttt ccgggttggg gcttggggcc ccgtaaaccc
     5641 cctcgcaggg agcatcgttg ccgttcagcc tgacagatag atcatgtagg gcgccggccg
     5701 gtgctattct gcatccttcc tgtggacgat tccagcgact ttgccggcat ccagcacgaa
     5761 tgcaattcca gtccccggtt tttccagttg tccggcagcg acgactgctt ccatcactgc
     5821 atcagtgcgg ctacgcggga tgagggtcaa aaccacctct ttttcgggct caatcagaat
     5881 cccgaagaag gtcttctgtt catgcacccc ggtgccccgt ccgtagagga tagtgccgcc
     5941 ttcggcgcct gcgcgccgag acgcctcaac aacttcgtcc gcccaacctt tattgattat
     6001 cgttacgatg aggtcgtatt cttctgacac gcgcatttcc tcccgtcttc ttttttcgag
     6061 ttcctcttaa agatgaagct catgccctcc acggcgagcg tggaagcgag agcgatcaag
     6121 catacgagac caaagcctgc attcatggtg ggccctccca gtttttcggc ggcgattccc
     6181 aggccgagcg acataaggaa ggtgactgtc aacggccctg tggccacggc gcctgagtca
     6241 aaggcgatag cggcaaaggc cggatcaacc catttgagca gaatgagagc tataatgtaa
     6301 ccgggtatga ttatccatgt cagtgcgatg ccgcgggcga taaaatagta gcccagtccc
     6361 accagcacag caactccgct ggccattgtg cggagaagga ccgattcgcg tatagaccct
     6421 gccgaggcct tttccacttc accgctgagc acccgggctg acggttccgc gagaaccacc
     6481 agaagcccta agatgaatcc cacgggaaga gcgtaaatag gattggacag cccgcctatt
     6541 ttcgcgccca gcatttttcc gataggaagg aatgctatgt tcactccctg caggaaaatg
     6601 gagagcccga tgaacgtcga tatcattccc acgacaactt tgcgcatgaa cgtgcgcggc
     6661 agcttcaaaa agaaggtttg gaatatgaag aatagaattg ccaggggaat cagggtcatg
     6721 cctacttccg ccatgactgc ggggaagccc tgaaagacga tgatctcgcc catcagatga
     6781 acacccccca gatcatgagg accaggattg gccctaaagt agccaaaccg acgagtccga
     6841 atccgtccgc cagcgattgc ttgcgctgaa gaactgatgc ggtgcccagt cccagcgata
     6901 tgatgcatgg tactgccatc gggccagtcg atacgctgcc cgagtcaaag aagaacggta
     6961 ccaattcggg tttggtgaag aaggacagaa ttatcgctgt agaataggcc cccaggaaaa
     7021 tgtattttat aggaacgttc agaactatcc ggagtatggc gacagttacc agcaggccca
     7081 tacctgcgga gatgaaaatc gccagaaagt cggaattgat gcccgcgtcc gggaaggacg
     7141 caacctgcga cacaagcacg tgcaccgaag gatctgcaat ggtgacactg aatgtaaaga
     7201 taaaggacgt ggccaccagc agcgccattg agccatggcg aggcaggtag gcgccgatca
     7261 tctcgcccat tggcaacagc gcgattcgtg tgccccgcag gaacagaaaa agccctactg
     7321 tagtgaaaaa tgcgccgata aggagctgta taaggttagt ccatgagaag cccaagaaga
     7381 tgatgctcag cacggccact gcgatagtga gaggcgctac tgctgctaca acttctgcga
     7441 tggcttcttg gagcggagta gacactgggc aatctccttt ccgagtgtaa tgagttttgt
     7501 tagatgggct actcccctat tatcgcatat atgagagcaa gggggaaatt cggagctgtt
     7561 cgcctgctcg aggatgtctt ggggttccac ccgccaggga ggcaacgttc tgatgcaacg
     7621 gcctcaatcg aaatcagtgg gcgcttgggg gtcagcgtga agctgaaaaa ggcggcggat
     7681 ttcgtctgga gtcacgcgcg gtatggagag attgggaatg cgatctgatg gaaagaactc
     7741 cactgcatca gtctctatgc catcggggga aggcgtagag ggctccccga ttatttcaca
     7801 ttcgaaaaag agcttgtaga tgtgataagg cgctggcgga tgcgggtgct tcccccgatc
     7861 atataaggcc agaagcctcg atgcccgtac tctgtaacct gattcctcga atacttcgcg
     7921 ttccacggct tcgctcggag attcgcctat ttctgcccat cctccaggaa gagtccagca
     7981 cccatcggcc ttttctttca ctaggagaat tgagctatct gcgaatatgg ctccccgcac
     8041 atcgattttc ggggtggcat acccggtttc caatgtgtaa atatcccgga ctatctcggg
     8101 atcggcatgg gcttgctcgc tcatcatctc tgtggcgatg gatctgataa gctcgtatct
     8161 ttcgatatcg taagggccat gtgcataaga gagcccgttc tgcgccaccg cctgaagtcg
     8221 aaggccccat tccagccact tcgggtccat gattcgctcc cccttccctt aacttgcaag
     8281 ttctccggat tgcccgagac tcctgccggc atgtcttccc aagtcatctg caactgcctg
     8341 aaacatcgcc gagcctgtta tgggggatgg gcaggaaacg caggagggcg ggaggactca
     8401 tcgcttaaac gttaaaggaa tctgaccgaa taatggagga tatgcnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnngtcga tcatctcata tggagaacag aggccttgct
     8581 tggcaggggc gtgtggttgg ctgccgtcac aggaacaaat ttcgtcaacc ttgacaggca
     8641 gttcttttcc cttcttaatc cgaaggtgaa gacgaatctg ctggcgtgcg tgaacagagg
     8701 atcagaggta tacggctttg attctgaggg acgccctgtt aggctgtggg cccgggaggc
     8761 aacgtgtgcg gagaatacaa tgatggacca ggcggtcacc atggcgcagc gtgttttgaa
     8821 agagcagtac ggccttgcaa cacggattgt ctccgacagg atgaacaggc gcaagcttga
     8881 cataatgccc tttcccgaat gggaagaccc tccgaaggag cggattgggg agcttctcga
     8941 tgaaacccag gggcgcttga ggattgcagg ccttgaggga atcgagtcgg tgatagacct
     9001 ggtgggccgt tgctctgaag aggttgggct cgatggcagg ataacgagtg atgtgaaaca
     9061 tgtcgagctt gggctcacag acaaggctga ttccgtagat tacctggtcg aagaggtcgc
     9121 aaagacgcat ggcatagagg tgtcggagat tcttttcata ggagacgaat tcggatcaat
     9181 cggcggatgc gccggcagcg attcgaagat gattaccccc caaacagaat cggcgagttt
     9241 catcagcgtg ggacaggagc cggagggcgt tccgcctgcc gtctgtcaca tgggaggcgg
     9301 gatcgagtcg ttcgcgaaga tactcgaggc ccagatcgag atgtggaggg acgatgagga
     9361 gaacatgttt ggtcgagata gactcacaga cagcctgctg cttctgcctg cagaggacaa
     9421 ttgggtcgct tacgagacat gctgcgatgc cgagactgct gcatcgtatg agacgctgtt
     9481 tgcattgacc aacggttatc taagtgtgag gggtgcccat gaagacggtc tgtatcgggg
     9541 taacgcggga tcgtttgtcg caggggtctt cgacaagtct tcgggaccgt acgatgtgac
     9601 ggaacttgtc gtgattcagg atcctctgcc cgtggagatt gtagtggacg gcgagagggt
     9661 cgggaatgaa ggggtcaggg attaccgccg ggaactggat atgcgcggcg ggctcctgcg
     9721 ccgtttttac agatggagcg atggcgctgg acgggtagtg agggtagaga gtgaacgctt
     9781 tgtatcctcc gtggatgagc acttgctctg tgcgaggctc tccgtgacca tggagcgggg
     9841 gtcggggagg gtattgctcc gatcccgcct ggacggaaat gtatccaatt caggcgcggt
     9901 tcacctgaag gtagattcag ttgcggcccg gggcgatgga acggcggagg tatgcacggt
     9961 gactatggag tcgcaagcag gcatagccat ggcttgcgcc cagcaagcaa gcttcgttga
    10021 ggccccgggc aaagcccgtg gtgagcccga tgaggcgctt ggaacccccg tttatccccg
    10081 gcctttctca ggtgagtctg ccgctggcca cgattttgag gttcggctag caaaaggtga
    10141 ttccatcgaa atttgcaagt ttgtagctgt gcatacctca cgggatgtgg cgtggcgtgc
    10201 ctgcaaagcg ccgcctgcga tggatcagtt gaggacggca gcgcaggcga gtgcggagga
    10261 agctgcatcc cgcgggtttt cggggtgcag actagagcat ctggaggaat ggcgcagaca
    10321 gtgggaacgc tgtgatatcc gtgtggacgg accggatcgg gaccagatgg ctgtgaggtt
    10381 tgccgtgtat cacctggcgt cgatggggcc tcagagcgat gacagggtga gcattgccgc
    10441 caaagggatc catggcgaag gctacaaggg ccacgtcttc tgggatacag agatcttcat
    10501 tcttccgttc tatgtagccc aatttcccga tgtggcccgc cggcttctca tgtacaggta
    10561 tcacacactg cccggggccc ggcgaaaagc tcgggagaaa gggtatcgag gggccatgta
    10621 cgcctgggaa agcgcagatt ccggcgatga gactaccccg aagtggtcag ccccacatcc
    10681 ggagacaggg gagagagttc ggatttggtg cggagaactg gaggaccata tcaccgcgga
    10741 tgtggcatat gcggtatgga attactgtcg atggaccggg gatcggggtt tcatgaagga
    10801 gcacgggatg gagatgattg tggaggccgc tcggttctgg gcgagccgcg cccagtggaa
    10861 tgaggagaaa agcagatacg agatactagg tgtgatcggc ccggacgaat tccatgagca
    10921 cgttaacaac aacgccttca ccaattacat ggctagatgg acattgcttg cagctttccg
    10981 gcttgccgag gattttccga taaagtggga agcgctactt cgccgactcg gcattagcga
    11041 ggccgaactg gacgagatga ggagagtgac caaccttctt taccttccat tgccggatcc
    11101 ggtttcagga gtgatagagc agtctgaagg atatcatggt cttgaggacg tggacttagc
    11161 ggaactgaag gcaattgctg aggattctgg aaagtccgtg gaagagaact ttgggcgcga
    11221 aaggctcata gggagcaagc tgatcaaaca ggctgatgtg gtaatgctgg gtcgcctctt
    11281 gccgggcctg ttcggtgagg atatttggaa ggcaaactgg gactactacg aaccgcgaac
    11341 cactcacctg tcgtcgctca gcgcgtccgt ccattcgacg tttgcgtcgc atctgggctt
    11401 gcctgaggag gcatatgcct acttcgagcg gtgcatctcc attgagcttg aggatgagaa
    11461 gcgaaacctt gaggatggga tacatgctgc gaaccttggg ggaatatggc aggcagtagt
    11521 gcgcgggttt gcggggatac agttgctgga tccagcgagc caggatgggt cctgttgtgg
    11581 gtgcgggaaa gaggctgacc cggtcatatc catcaatccg cgtctcccga agacttggga
    11641 caggttggag attccgctgg cgtgccgggg caggcaccta cgggcggtca ttactcccta
    11701 tcgcgccgct ttcgccaatg agacgcagga tgccgaggca gacggaggcg ggtcggtgat
    11761 cattgaatgc gtcggacaga ggaagcggat cgcaccggga cagagcaccg aatttgtcct
    11821 cgactgaacg tcagttgagc gaaggcagac aagtctatgc tatcccaact tttgcatttt
    11881 caaaaacaat gcctaactca ccggttgcgg atgttgggtt ggttgaccac ggagactttt
    11941 gcaagatctc tctgccttcg gcaaggaacc cttcgcaaca tatatacaac tgaaacttcg
    12001 aatgatcatg gccgtgcgaa atagtagggc aaggagggag gagcagatca tacggatact
    12061 gccgttcacg aatgacttga gattgcatga gaagatcaat ccacggccag cattcttttt
    12121 gataaggaaa atacgtgtat gcagtatcta tctgtcagaa ggctgttgag aagacgatca
    12181 aggcgtaggt tcagtgaagt gtctcccagg aagcatgatc ttgtccggct ggcagacgag
    12241 gcggctatga tcaacgttga ccgaatggat agagaaccag ttgaaaccgt ggtatagaag
    12301 tatgtggagg tttttgggca acacaacata aaggtagctc aggcgatttt atttggttct
    12361 tacgcgtttg gaagtgccga cgggaacagc gatatcgact tggcaatcat ctcgccagac
    12421 ctcggccatg accgtttatc cgaagccata gtgcttaagt tgctaaccga acatgaagat
    12481 accagcctct ttgctcaacc ctttatcccc gtgaagctga tcccttctac gaagtagctc
    12541 tggaacacga tgaacaatac gatgatcggc agggctgata ttatcgttcc ggccaacatc
    12601 ggtccccagt cgaacgccac tgcgtaggtg cttctgaagt tgagcaggcc gatggtcaat
    12661 gtgtacttgt caggcggcga tgtgagtatc atgagcggcc agaagaactc attccacgtg
    12721 ccctggaagg tgaggattgc aagcgcaccc agggatggct gagccagcgg cagcatgatc
    12781 ttgaagtatg tctggaacgt gctggcgccg tcgattctgg ccgactcctc taggtcgtgg
    12841 gggagggatt cgaaaaactg tttcatgatg aagacggcgc ttccgttgac catggtggat
    12901 atgatgtagc cgggccaagt gttgatcaga gtatccactc cgaagagttt cgacagtccg
    12961 aaaatgccat cccgcaggac taagtagttg gatatgaagg tgacctgccc ggggatcatc
    13021 atggaaaaca gcgttaggaa gaaaagcgaa ttccggcctg cgaaatcgag gcgcgccagg
    13081 gcgtagcctg ccattgacgc gatgaggagc gtggttacta cacgtatcgc gctgaggagg
    13141 aaggagttcc ttatccataa gagaaagaga ctctttcctg ttgtgcggct gtagttctcc
    13201 ctgaaaacgc gatgataatt atagaagacg tatgggatga tgccggatgt gacgttgttc
    13261 cagctttgga cacgtcccag cctttccagc ctgttcggcg ccaacgtggc ggagacgaat
    13321 ttctgataga gcgggacttc gacatccatg ggaagcctgt cgtatgtgag ctcgcctgta
    13381 tgccttatct cccattcata ggtcactcgg accctcccgt ctaccttccc gcgggacacc
    13441 tctctcacct tgctcactac agcttcatct gcggcaaacg gcccaagcct cactgcgccc
    13501 gctccggctc ccggaacgcg cctcggcact acaacgattg gtggttcggg gtcttggcct
    13561 tcgtctacct cgtaggttgc gctgaacggg acaattgctc ccggagcgaa agccccgaag
    13621 aaaccgtctc ctgctcccgc cttgcccagg cgataggctg caccccagtt caccggcgaa
    13681 agttgtggtg agctgagtgt tggaggccat tcggtgggat tgtcctttag gctcgacagg
    13741 aatgcaaaga cgagcgggcc catgaaaaag atggagaaga acatgagcag cacgtatatt
    13801 gaggccgtcc gcgcccatct tcttcttgcc atggcctttg atggctgatt gctccgtgcc
    13861 ccggccgctc ttctgtcaac tgctctgctt tgcatctgga tcatcctcct gacttccatc
    13921 ttggggttgt tcggtcgcca atcccataga tatggcctgc attagagtta agcccgcgag
    13981 agctttcttt gaatccacgt aagaatgaac gtaaaggtcg ccaaaaccat ggccgcagct
    14041 gaggccgcgc ctattctggg cactgcagaa ccagggaaag cattgtggta tacgaagtag
    14101 gccaacgtta ttacggattc cagtggcgct tgatccccta tgatggccac ctggtcaaac
    14161 atctgtagtg ttccgatgag ccccatggtt acaacgagat atgttacgtg tttcagccca
    14221 ggcaacgtta tatggcggaa cgcggcaaaa ccggtagctc cgtcaacggc tgcggcttcg
    14281 tagagttcgc tgggaatgtc ctgaagaccg gcgagaaaca ggagcatcat ggtgggcgct
    14341 gtggtccagg tgttcagtat cattatcgct acaagcggac gtggaattcc cagcttgcct
    14401 gcccattctg ggatagtctg gcgcgtgttt agccagatgg tccttacagg ctcaacgcct
    14461 ggattgggcg atactacccc tgcgaactgg agaagccaga ccacgaccaa tgacagaagg
    14521 gccgacgcca ggatgaagct gggctcgaac cacgaacagg gctgcttgcg agtccgctcc
    14581 gtaatcacca tgagaacctg gagcacgacg agggaggcga agaatgctcc tattatggct
    14641 ttgcgctcca gaaaacttgt cgttaggtaa ttgaatattc cgttgcgctg aaatagccac
    14701 ataaagatga gggtgatgac cacgctcgat gtgacactcg gcatgtagta cagtgctctg
    14761 aagaacttga tcccgcgtat cttggtattg agcactatcg caaggatcag ggcgaatatg
    14821 gtttgaaaaa atgtgacgat gatagagaag gttaccgagt tcttgaatgc ccgcccgaac
    14881 tgcacattcc ggaaaaggtc cgcgtagttc ttcagcccga cccacacggg cttcgagaac
    14941 atgttgtaat ctgtgaagct gaaatacagc gtgcgcagga acgcatatcc gaagaatatc
    15001 gcgatgaaga agatatacgg cgcaagcatt atatagggag aagccttttc caagacggcc
    15061 gatgattgtt tgggtttcac gatatcacct ccagagcact ggagctacgc atcgagggaa
    15121 tgaaacggtg aggcagggag ggactgtcgt gcgtagcgcg gccggccaac atcagcctta
    15181 ggccgcttgc ccgaccgtcc cgctcggtca gcgagtctcg aagcgcccca tggggcgctt
    15241 cgatcccgcc gcgctgaccg cgttactttc cgatcagctc attcaatctc ttctgggctt
    15301 cttgcagcgc cgcatctgca gactgttgtc cactcatgac cgagaccaga gcgtcattga
    15361 tgggcatcat ccagtcaccg ccgtactgac cgaattcaaa aggcatcacg taaccgtctg
    15421 ctgccccttc aaagacgacc tggttcgcaa cggcttcctt gtctgtcttc ttgaaatagg
    15481 ggttgttgac gagggcccgg cgtgacggaa gcgccagccc gcgttcgaga acccattgct
    15541 gcgcctctgg agatgtcagc aactccagca ctttgaaagc ctcgtcttta tgcttagagg
    15601 ctgcgttcat cgaccaagac actgtgaaag tgaggttgcc gcgctggcct gttcccgggt
    15661 atttaggcag caaagtggca ccgtaattga ggtttgggga ttcatcgcgg aggaagccta
    15721 cgatccatgc tccctcaaaa cagcaggcta ctttctcgac cgccagggcc ccgccgcccc
    15781 atccttggcc gatatctgcc ggcattacac cgatgccctt tgcagcaaga cctgtatacc
    15841 agttgaaagc gtcgcggaac tccttgctga gaatatcggt tttgccgttc ttgtcgaagc
    15901 tcctgaagcc ggtggcgtat gcgaacgcgc ccattcttgc gaattcaggc gacaagcaaa
    15961 caccgtacac tcccgggaag gctgcctgga ctttctgaag tttggctgtg aaggtgttcc
    16021 agtcgtcatt cttgtcggga tagggaactt tcgctatgtc aaacatgtcc ttgttgtaga
    16081 tgagggcgag tgtattgaag tccttcggta ttccatacac tttgcccttg taggtgaatg
    16141 cattgaggag tgcagggatg atgtctttcc cttgcaggat gttcgacttg gccaagtagg
    16201 gatcgagggg ttcaactgct cctgatgaga ataggccctg ggcccagaag atgtcgacgt
    16261 agaacagatc cggcccagtt cctgccgata acgcgttgac aatccatttg tcataagcgt
    16321 cgggtattgg ctcgtaagct actgtgatcc ctttcaggtt aggctgcacg aacctggcaa
    16381 tgagctcctc aacgattgcg gtgtcggtcc cgccgaaacc tgctacgcgg attttgacat
    16441 tggcggcctg agatgcggtg gcgcatgcca acatggccgc aagtatcgct acgcacaaca
    16501 ctctgcgaat cttcatcttt aaataacctc cttgtgaagt cggttgtgaa attcgcttgt
    16561 agtcaggtac aggggcgatg cccgtgttaa ttcggagaga acacatatca cactgcactt
    16621 tgctaatgta gtagctgtat tctgtgtgcg cgggtctgac agggcatggc tacgtggttc
    16681 atattcgctg ggttgctgta tgctccatgg ctgtcagtgc gctttctgga ccacctcccg
    16741 ggcaggtctg cgcttatttg gctctgctgt agagctacgt actatcagat ttgccttgag
    16801 gagaacgcga gatggcggtg tttccccgtt gcacgcagag atcagcatgt ctgccgcagt
    16861 ctggcctagt tcgtaacggg gttggtcgag ggtggtcagg ggcggagagg acattgcggc
    16921 catatcaata ttgtcaaacc cgactacgga tagatcgtca ggaacagtaa gtccccgttc
    16981 gcgggcgcct tccagcacgc ccatggccat gaggtctgat gccgcgaata cagcggtcat
    17041 gtccggagac ctgtctatca tggaaaggaa ggcctttttg cctcctttgg ctgtgaagtc
    17101 ggcctcggca actagcgagg gatcgaatgg aatctccaga tcgtccaggg cgttcctgta
    17161 gccttggagc cgctcgaggc tgacagaggc ttgtttgtat ccattgacga taccgatcct
    17221 tctgtggcct agatctgcca ggtgcctgat ggcggaatgg accgctgaca ggttgtcgca
    17281 tcccactgag aacgtcatgt ctccggctaa gggcatatcg atggtgacta tcggaattgt
    17341 cgatgttgta aggtctgcga gccatggatc atcggtgcgg agtcccatca gaaatgcgcc
    17401 ctctaccatt cgtcgcctgc agaggtcgag gtaagaagag ttcgttgagt cgggcggacg
    17461 gttcgccttg aacaccatca tgtcgtatcc ttcctcggta agctcatcaa ggaatccatt
    17521 gatcacttgt ccggaaaacg ggtgagaaaa gccttcgccc ttttccctgc ccaacacgaa
    17581 aagccccatt accccggact tctgagtgac cagggtacgc gctgctatgt tcggctggta
    17641 gttcagctca catgcgatgc ggaggatgcg gctccgcgtc tcctcgctta tgtctgggta
    17701 cccactcaaa gctctggaga cggcagtcac tgatacaccg gcctgttctg caatatcctt
    17761 tagcgtggtg cgactcatgc gttcgtggcc tcctcgtttg tcgttcgcaa ttgttatgtg
    17821 gggcattggg cctccacgga actaaatcga ttaagcaaca acattgacga taaaaactgc
    17881 gtttgcctgc ggttatgtgt tgccaagttt ttaaacgcca aggcgctact gcgatgcacg
    17941 ggcaaaatca cttaaacgtt aaacctaatg aacactattc tacatggggc tgaaaaatcc
    18001 tacctgggtt tgaaataaga ttggggggat ggaatggccg gttgggtgcg gccgtgtggg
    18061 cgaagtatag gcgaataacc tcagactgaa accgaaaccc gttttctgca tctttccgta
    18121 gcactatttc tccccaagta ccgaaggatc tgcgcccaga gaacaagagc gcgctacgcc
    18181 ttgattagtc ccgctgaact gccggacatg aggagtactg tcccctcatc gtctactttt
    18241 tgtgtatgta gcgaggaatg gcccgatatg ggccatttgg tcagggcttg ggccttcata
    18301 cccacccccg cctcaggctc gcacagtcgc attcgtatgt gcgttcaaat gaatgaaatc
    18361 gccgctacct tcggtgcggc ccgtagccat aaggctttgc tataggagtt cgtagccgga
    18421 ggcccttttc tctcagggcg tctgccatgc ggcgacatac aggacaaacc tctgggattg
    18481 tggtggattg agccgctgcc tatgattggc ttgtgtcgat aaattcggca catcagacaa
    18541 tagatgccga aaagtgacct gacgccgttt tcttggccac ttgggcgatt aaccccgtgc
    18601 agagcgcact agtttcaatg catattcgtt ttccaacgtc cggattgacc agctcgtgtt
    18661 cgtgccccta aacagcggca acgcagtagc taagtcccac gactggacaa aaagttatca
    18721 tcatccggat cttgcaggta gacgcggtcg aacgcacgac ttttctgtgc cagctcaaac
    18781 tggctatatg cggcctttct gcagtcaggg catgcctgcc acggcacgag catcgcggca
    18841 atctgggcgg ttcttccggt agaggcatga agcgcgtgag agaagacagc tctccacccg
    18901 ctgcatgatg gacacaaccg tatctgctcg cgttgctgtt cgactatgtt gtcggtgtcg
    18961 tagtagatgc ggcggccgcg ttcgaaatac tctcctttgc caaagatcgc gccgatgtcg
    19021 gcttcttctc ggtcagccca catctcctgc aatttgcctg tgaggcggtg aatctcttca
    19081 gttacccgct gcggctgcct cgcgatttcg ggatcccagt cgggctcgcg tacggctgag
    19141 taatcgaggc ccgccagagc cagcagaatt ccgacgttca cgtatggcag tgcgccttcg
    19201 atggagtatc ctccttcaag cactacgata tccggggcca agagatcggt gagccgggca
    19261 tagccgaagg cggtgaagtt catattggtc agcggatcgg tatagtggtt gtcctgccct
    19321 gcggcgttga cgatcacgtc cggctcgaag tccttgagta tgggaagaac caggttctcg
    19381 aggacataca gtattccctc gtccccggtt ccgggaggca ggggcacgtt caccgtcatg
    19441 ccgtaggctc gagggccgcc tctttcgtcg atgaacccgg tgccggggta gagggttcgt
    19501 ccatcctgat gcaagcagat atggagaacg cctgggtcgt tgtagtagat gtcttgcgtg
    19561 ccgtcggcgt ggtgggcgtc ggtgtcgact atggcgatgc gcatcttggg gccatgggtc
    19621 cggcgtatgt gctctaccat tatggcttcg ttattgatgt tgcagaatcc ccgtgagccg
    19681 tggacgattc tcatggcatg gtgccctggc ggtcgcacta gcgcaaaggc acgatccacc
    19741 tctccgctca gcactaggtc ggctgcgagc atggcgccgc ctgcagcgat tctgtgggac
    19801 tcagttatca cccggttcac agttgggaca cacacgtgcg cccgcagaat atcggtatct
    19861 gttgcgatcg caggacggta ttcttccact ccgggcacat ccaggaggcc ctcctcaatg
    19921 atctggtcgc gggtatagag gagccgttcc tcgcgttcgg gatggtccgg agttatcgcc
    19981 cagtcgaagg cggggaagaa gatcagtcct acacgagttt tgcgctgttc catttctaaa
    20041 catcctcctt cgactgatca ggtccttcga cgcctgaggt ttcagctgct ttgatgcgcc
    20101 taacttgtgg ccgcacctgc gcttttatgg agtatatgtg gcctgcggtg tagaagccgc
    20161 gcacgacatt gaagctttcc tcttcgacga tggaaacgcc ctgcgaagcc tctacgccga
    20221 tctcttcggc gaaccgacag gcccatgcga tggcttcgga gcgggcctgt ttgatatcga
    20281 atgttatcgg gcgctttatg cgttccacat ggtcggcttc aggaacggta agtacgccta
    20341 gcattgtgtc ggcatgaagc gtgattgccg cagtgggctg gctcgctgcg gcgccaacgg
    20401 cattagcctc ttcgtagtaa ggcagaatct cccatgatat cccaaggcgg tcggccgcgg
    20461 gcgggatgaa aacaggcgcc ggtgcgccga gccctatcag catggcgggg cggatatcgg
    20521 ggggcgcaag tatctccgag acggtatatg ccggtatcga ctcgagatgg gcataaacag
    20581 cctcgatctc atctgccagc tggcgggtga atgcatcgag cactgccttt gccaccgacg
    20641 aagcatccat gccatatggt gcgcccaatt gcgccatggc cctggtggcg agctcgcggc
    20701 ttccaaggga cgaaagcccc tctgctaccg ctgcatcagt aggggtaggc ttcgagccgc
    20761 cgcgaacgac cggaactccc gcacgccgcg gaccaacata gaaagtggga gctccattca
    20821 cgaggcggac atggatggag ctatcgcctc cgagtccgat tgaacgggaa aagagcgccg
    20881 ggaccaacgt cttgtggccg gatatctccg ctccgtagcg gtcgtatatc ggttctccag
    20941 ccacctgtac ggagatatcg gtggtggttc ctcctatgtc gactatcacc gcgtttgcgc
    21001 ccgggtgacg cgtgagcgct ttggccccca ttgtgctggc tgccgggcct gaaaggatgg
    21061 tttctatggg gcggatcatc gattctttga gccgcatagt gccgccgtct gccttgagaa
    21121 tgaagacgcg ctccacctcg tctcctgtta tgaggttttg taccatctct gcaaagaggg
    21181 attgttgccg ggctatgctg gcgttgaggt atgccgtggc aagccggcgg gggaaattgg
    21241 ggcgtccgga gagcctgtgg cctaaagttg caggagagta ttgcggaaat tcggctataa
    21301 cttcctgttc tatctggagc tcgtgggcgg ggttgcggtg agaaaacttg cccacaatgg
    21361 ccagtgcctt tgcgccgtca tcgcacgcag ctttcagtgt ggacatggcg gcagagacgt
    21421 ccacgggtgc cacttcgcgt cctctgtgat cgatataccc ggaaagcgcg tggacaggga
    21481 agccgagccc catatcgtca aaggacattc ccgggcccgg cgtggccagg acgacagtgg
    21541 gatctccttg tccctctatg atggcgttgg tggacagcgt ggtactgagg tgcagctcaa
    21601 tcgggctagt gccgtcgtgc gcctccacca cctgcctata tgcgatttgt gcgccctcca
    21661 ggagattatc gggattagtg ggcgcttttg cggctgcaat cacatcttca tcccttacca
    21721 ggacagcgtc tgtgttggtg ccgcccacgt caatgcctac aatgtcgcaa cgattcctca
    21781 aattgcccac tcccatgaaa gactcagtct ctactttact tctattatct tcttcaaagc
    21841 cgcctgattt gcctgcccgg gagaaaagtg gggtggagca tatggcgtca tgggcgagtg
    21901 cagggtagaa taagacttat gaatcgtggt atgcagcatc tgcaggcact gatagaatat
    21961 tgacatgctg gacagcatgg ggaggaatga atatgcagaa gctttacagc ggcaagacca
    22021 aggacgtata ccttgctgat gacggcaatg ttctgcttta tttcaaggac agcgttacag
    22081 gtgtagggga taagatagat tccggcgcca acgaagtcat aggcgaagtg gcgggaaaag
    22141 gacactcgtc cttcagtttg acgctgatgt ttttcgatct acttcataag gcgggaatac
    22201 ctacgcactt cattagcgtg gggccggaag acaacacgat gctggtgaag cgagcccggt
    22261 ccttgaatct cgaggtaatc tgcaggaaga aggcatgggg aagctttgtc cggcgctacg
    22321 gggcgtatgt ttgcgaaggg caaccgcttc cgtctcttgt ggagttcacg ttgaaggacg
    22381 atgatcgcgg ggaccccctc ataactgagg atgccctagt cgctctcaac atagcctcca
    22441 ggcaagatat agactacatg aaggagacag cccgcagggc gacagatctg atctcggggt
    22501 atttggccga ccgcggcttg gagctgattg acatcaaata cgaattcggc gtcatcgacg
    22561 gaaagaccat gattatagat gaagtttccg gtgacagcat gcgggttgta aaagacggaa
    22621 aggtcttgct ccagaacgag ctgtatgagg ctctccttgg acgatagccc atggccgtgg
    22681 acagtcgagc ggggccggtt ggaggccgat tcggcgaagg ctggatcagg agctacaggc
    22741 agggcaattc gcgcatacat ctactggcag atgagtgcag tgatatgtct ggaatccact
    22801 aacatgaaat gtcatgtaga gtggattcgc ccatgggccc ccgcccgact gctattgcca
    22861 attagcggca tttgcgacat ccttcagtgc accttccttg gaattggcgc agggaacagg
    22921 tacctggggg gcacacggag gatacatctg ggaaaactcg gtcgtaactg cgattgcaac
    22981 tatgaatgag ccgactgccg ttatccagcc gcctatgacg cgcacgaaaa aaagggattt
    23041 tgctgtatcc gatgacccca tatgagagtc ctcctcgaga gctcttttgc tctgtttgtt
    23101 aggggcagat atgtttggcg caggaaggaa tctggtttac tttgctgtcc ccaaagactg
    23161 ttttcgaggc ggcagccagc ttatccgtgc cgccatttgg gatgagctga aatcccaagg
    23221 tttttgacgc gcccagtctc agcttcctta cctatttgac ctgccttcat ctcgtctcga
    23281 ttagtgccaa acaccagtat caatgaatgg gcttccagaa tgtggcggta taatcccttc
    23341 gcccttcatt cagtatgggc cttgtcccgg cacacttcga atgtgccgac tgcactattt
    23401 ccccggtgga aaagtgggct agtggcgtag ggctaatcag tgccagcgcg cttgacttca
    23461 atcacatacc gtcctgaagg atcaggatca tgcttcgaac agtcatcttg gaagaattcc
    23521 agcgttacgg cgacagccac gatgaacgag ccaacggcga gaatccagcc gcctatcact
    23581 ctcaggagcg cgagtgtaga cttgcagctg tttgacttca tgtaaagtgg cctcccccca
    23641 atgctttcgc caagactgcc ggcgctgtcg ccttcacctt gcatgtacct tatacgcaag
    23701 ccatggctca agtgtgttcg cctttcggcc cttcagactc tctaccgcgg gtggagcccg
    23761 gaagatgagt ggcgtcggaa cggctcggaa tatctttcaa gtgaatccat caagatgcag
    23821 gtctgcgtat tctcgccgca aatatcggtg ttgacctggg aaccggcggc gtatatagtg
    23881 tgtatagacg atatacacgt atttgcccgg tcggcgtgca ggtgatgcgc aggagagcca
    23941 tagttgcatt cgggtgatgc ggcagacgtt tctggcgcct gcagtgcgcg attcgggacg
    24001 gttaggttgg gatgacatgg atatcgccat ttcgccgaac aatccagtgc ccatgtacca
    24061 gcagatagtt gaccaggtta gggcccagat tctctcaggc gagctcaagc cgggagatcc
    24121 ccttccgtcc atcaggaagc tcgctgcgga tctgcttgcc agcgtgatca cgacaaaacg
    24181 agcctatgca gacctggagg cggaaggatt gatcgtgaca aggccgggcc tggggacttt
    24241 cgtcgctgat ctttctcttg aggatattga acggctcaag gccagagagg tcattgggca
    24301 tctggcgaac gcggtcgagg aaggccgcag tcttggactt gaagacagtg aaattgcgga
    24361 attgctccag aacgtgctgg atgggagggt caactatgga tgatgcagtg cttcagctct
    24421 gcaaaatctc aaaggcatac ccaggctttg agcttaagga tgtctctttt gaactgccgc
    24481 gcggttacat catgggcctg gttgggccga atggagcggg gaaaacgacc atcatcagga
    24541 tcatgatgaa ccttgcgcgc gctgattccg ggcaagcagc catcttcggg ctcgacaatg
    24601 tagcccacga gaccgaggtg aagaggcgca taggctatgt tcctgaaaac cacaacttct
    24661 acgacgacat gactgcaggt tggatggcgc ggttcgtgcg ccggtattac cccacgtggg
    24721 atcagaacta cttcgacgcg cttgcggcca ggttcgaaat tgatctgggc aagaaggtag
    24781 gggcactctc caaggggaac cgggccaagc tttccctggc gttggccatg gcgcacaggc
    24841 ccgagctgct catcctggat gagcccacat caggtctgga tccggtggtt cggcgagagc
    24901 ttcttgcgga gctgctcgat ttcatgggcg acggcgaacg gtcggtcctg ttctctaccc
    24961 atatcacttc cgaccttgac cgggttgcag attacatcac tatcattgtg gatgggcgga
    25021 tacatgacag tgatgagaag gatgccttgc ttgagaaggc acgcaaggac gagccgacag
    25081 cgtccataga ggatatcgtg attttggcag cgagagggga aaagtgagat gacgaatctc
    25141 atatggaaag atctctacgt aaacaggtcc atgatattca ttgcgatagg gcttttcggg
    25201 gggctcttcg tttttggagc gctcacaggc tccgaagaga ttgccgcgta cagcttaggg
    25261 attatgaata ccttttcacc gatgatggtt ccgttctgtg ctctcaatag cgctgccaat
    25321 gaggaaaaga accggaccct cgccctgctc cgatcgatgc ccatttcccc gcggacgata
    25381 gttgcctcca agttcatctc atctttgctt gcaggcattt tgtggtctgt catcaatatg
    25441 gtcgcggcct cgtggggaac caggcttttc ccaggggctg ggggcgagtt tcctccgagt
    25501 tcattgacgg gcctatacat gatggccctg acgcttccaa tagctgccgt gacattgatc
    25561 gcgttcttca agtggaattc gagcgcagcg cggaacgcta tcatggcgct atgggtagcc
    25621 ctctatgtta tcccgacagc attccgtccg gctgggatga gcatgacgga gttctacctg
    25681 aagatcatgt ctccgactcc gtctatgata tcagttgttc tcgttgcttg ccttggagta
    25741 tacgttgtgg gcatgctggt tgcacaagcc gtatttgccc ggcgcgaact cgcttgatag
    25801 ggcgcgcagc gaggagccga cagcgtccat ggaggatatc ctcatttcga ccgcgagagg
    25861 agggacatga gatgatcagt cttgtctgga aggatctcta cacaagcaga ctgatgctgc
    25921 ttctccccat cgggcttttc ggcggactat tagtgtttgg agcgctcacg ggtaatgatg
    25981 aaatcgcatc attgtacttc tcgagtttag ccaacaccta ctccatgctg atggttccag
    26041 gctgtgcgtt tgccaatgct tcgaacgagg agaaaaaccg gactctctcc ctcttgagat
    26101 cgatgcccat atctccgcaa acgatagtcg catccaagtt cctcgcgccg ctgtttgctg
    26161 gggtgttgtg gtcactcatc agcgtcgctg ccctgttgct cggcgctagg ctttttccca
    26221 aagcagcggg gatcgcgctt cgatcgactc cattcctggg cccatatttg gtggccttga
    26281 cgcttccaac agctgctatg atattgatcg tatccttcgc atggaacgcg aacgtggcag
    26341 gcatagcttt cgtagtgtta tcggcagccc tctacatgat ctcggcaata tttcgcccag
    26401 ctggaatgag catcatgggg ttctacctga agatcatgtc tccgactccg tctatggtat
    26461 caattgcctt tgccgtttcc cttgcgatat actttctggg gatgctggtt gcacaggtaa
    26521 tattcgtcca tcgtgaactt ggttgagttt gcaagaggaa gcgggaatgg agttgtggct
    26581 acggtgtatg agcatatcgt caaccctggc agtattgtgg tagtcggagc aagcgatgat
    26641 gtttcaaagc ccggcggcag agtggcgaaa aacattgttg accacggtta cgccggaaat
    26701 ctctgggcgg ttaatccgaa acacgatgat gtgctcgggc tcgcgacctt caagtccgtc
    26761 ggtgatcttc ctggcactcc cgaacttgcg attatcgcta tccctgcacg cggggttcgg
    26821 ggggttctgg aggaactggc ggcagtcggg acgcgagccg taatcattct gagcgcgggg
    26881 tttggcgagg tttcggaaga aggaaagcgc gaggagcttg cgctggtcga gattgcagag
    26941 aggcacggta tgactctaat aggacctaat tgcatgggag tgctgactcc gtgctatgcg
    27001 ggagtattcg cagggcccat cccaaaactt gagccggggt gtgtcgatat cattagcgga
    27061 tcaggcgcta ctgcagactt ccttgcggag ctcgctatag aacgtggact actgttgtct
    27121 tcagtcttta ctgtgggcaa cgctgcgcag gtgggggtcg aggacatcct cggcatgatg
    27181 gatgagcact ttgggtcggg ttcggctaag gttaaggcct tgtacatgga atccgtgaag
    27241 aagccgcaaa agcttctgcg ctatgcgcgg tcgctgattt ctaaagggtg ctcgattgtg
    27301 ggcataaaag ctggcgcgac aggtgccgga aaacgggcgg ccgccagtca tacaggagcg
    27361 atggccagtg atgacacagc agttgaggct ctattcgaga aggctggtat cataagggtt
    27421 tccagcagat cagaattgct cgacgtggca agcgttttac tcatggccgg cccgatagat
    27481 ggaagaaatg tgtgcgtgct tactgatgcc ggcgggcctg gcgtgcttct cgccgacgag
    27541 ctggctcgac agggcctgaa ggtcccattg ctgcgggagg caactcaaaa aaggcttggg
    27601 gaagtgttgt ggccgggaag ctcagccctc aaccccgtag attgcctatc aacaagaacc
    27661 cccagccaga tccagcaggc ggttgacgtg atctcggaag aagagcgcga ccgtatggat
    27721 gttatgccgc tcatcatggg cgatccgggg atgggcgggg tcgctgagat gtatgacgtt
    27781 attgcccgtg ctatggacga gtccccaatt ccggttattc cagtctttcc atcccccata
    27841 acttcagctc tggcgctgga ggacttcaag gcccgcggga agtcctattt tgttgacgaa
    27901 gttcagctcg ggcggagcct ggggcgagtg ataaaccgtc cgcgcctgga tgagccagta
    27961 gagacacttc ccggatattg ccgtgaggcg atctcaagag cgctgcaagg ggatgaggct
    28021 cacgggtctg ctcatgcgga caacaccagg acggctttgg cgccccagac ggctgctgca
    28081 ctgttgcgtg ctgccggcat acctatccct ggccaagcag tggcagttgg agtcgaggac
    28141 ttggacggga tctgctcgga tctgggattt ccggttgtca tgaaggttgt gggccccctc
    28201 cacaagactg atgtgggcgg agttgcgctg aacgttgcga gcattgagga tgcccgtgat
    28261 acttggcgga ggttgatgtc aatagacggg gcaaccggcg ttctggtcca acccatgata
    28321 gaggggacgg aactgataat gggagcgacc cgcgaagagg gctttggggc actggtcatg
    28381 ttcgggcttg gcggagtctt cactgaggtc ctcaaggacg taaggttcgc tctggcgccg
    28441 ctggggatgg agaaatgcaa gagaatgatc gattccattc gcgggagacg cctgctcgac
    28501 ggggcaaggg gcggacgggc cgtcaatgtt ggcgcagcag ctgacatact ggccagggtt
    28561 gggcttttgg tctctgattt tcctgagatt gcggagctgg acctgaaccc ggttaagggg
    28621 tatgggtcag agctttacgc gatagatgcc cgcatcatag tagagagaag gcctgatgaa
    28681 ggcggggctt tggctcgctt acgcagcagg catcatgctc gctagagtca gggggcgagg
    28741 ccgcccggat tgcgtgggcg cgaacgcgaa cttttccagc ggggaagctg tggtggaacg
    28801 tacaggtgat gcttgggcaa tggcagtcaa gctcgacggg gtcgttgtgg agtatgagtc
    28861 gtgggaatat gaagggcgaa ggagaaacga agatgcgcgc tggatggcgt gaccataggg
    28921 tttgcgggcg gcgaaatcgt tgggatcgag gggcctaacg cgtctgaaaa gtcgacgctg
    28981 cttcgaacga tgtgcggaat acaccttccc atctccggcg aggtgttggt ttacgggaaa
    29041 aggcctgaca tctttgactc agacttcaag aggcgcgtgg gctatgtcag ttcccgtcgg
    29101 acatcttcgt ttgccctcca ttgcgagttc tacggtatgg acaagcacga gtggtaactc
    29161 ccgccttgac ctggaaccat tggcggcccg aaggcnnnnn nnnnnccatt gcgagctcta
    29221 cggtatggac aagtacgagt gacaacgggt agcgtcccgc cttgacctgg aaccattggc
    29281 cgcccgaccg ccgcgcgagt attcacaggg gcagagggcc agggctgagc tatggcttgc
    29341 gctccttcac agattggatc tggtgtttct ggacgaggcc acagaggccc tggatctcga
    29401 gtatctgccg ctcttcttgg gaacatgagc gggcaaggcg gtggcggccc gaaggccttt
    29461 ttggcccaaa gttgtgcgga tactggctaa gcagaggttc atgccgctgc cggattttct
    29521 aggctcagca ttaggagcca ccctcgtatt gttgg
//
LOCUS       DAOSYL010000027        27231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1451_length_27231_cov_7.291875, whole genome shotgun sequence.
ACCESSION   DAOSYL010000027 DAOSYL010000000
VERSION     DAOSYL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 27231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27231
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1451_length_27231_cov_7.291875"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..2698)
                     /locus_tag="PLU15_06055"
     CDS             complement(314..2698)
                     /locus_tag="PLU15_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOO30520.1"
                     /translation="MSEISKRLSITAISVLIVISLMSSAVLAAPVTIKDVPEDHWAYE
                     AVKLLVEKGYMGVYESGNFCGDDPVSRYLLAFVTAKLLRDVEAGRTTLSEDDMRVVRE
                     TATVLREQLAVVTARLEEVERIAAGAAGDAKIAQETSASVALNYSRLEQAMEEQRKQL
                     LAEIGKTAGASDQKLTAVFGDLKKQSQEIETELGQEISELAARADSLEAFAHRSFASE
                     AQSRADLEAKLTLSLNKISEDQRAYVAQMNELGARVNDVDGRLNALQSETGSRIATLS
                     LRLDDTAKGLAANKAWMESALAALDNKVSQLSDALDAQRVDTELKLGSMQAYQNGQLS
                     SAIEGERVAREKADAGLVQQIDALSLSLSKETQERKASDAEIAARQTELESTLNLKID
                     SAIQTERATRASELAELMARLESLTSSQDELEQTTALRLAAGIEREKADREKAQAGLA
                     QQVSALSLSLDEESKAREALGLDMLARQAQLESALTRKIDSAIEGENADRKAAYAELS
                     SKIDAFSLSLGKELETERAQREAADIAHQEEARRILDEIAAARRDITDIERKTAEADA
                     AIIAALEIQESRVAELGAELARLAADVRTLQEYAAGIEAELLQMSEKTSEDYASQQAA
                     LAELAKALDQTSADLSAAIKANAAEDLKTNGRVLQNTMRIQDVARSLDATNERLGQTD
                     IAVRQAEGSIEEITARLAEIDSHLASIDRLLAGSVDELAKRFSDDLVVERWEAEMREV
                     KLQSMIEELEARVEKLEGEKAQADAKKPGSAGAIVAVLGVIAAVAAIAIGSGGK"
     gene            complement(2726..4738)
                     /locus_tag="PLU15_06060"
     CDS             complement(2726..4738)
                     /locus_tag="PLU15_06060"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOO30521.1"
                     /translation="MVRSCKARAVVTALVLLWCAFIMHPGISRADTAAPVEISNQFIR
                     ITVNPGPAEAGRFGLETTGGDPRNPADDGLPLIYGRPMPWTSYTTVRIDGSDWVFGGK
                     TTRRAGRSGMYGEQVRPPQIEDGKVVCSYMLGDILVTQELSFVKSPSTRYLDTARIHY
                     KLENTGSVTRSVGLRLMIDTMLGANDGAPLRVGEKTIVSDTMLSRREIPDYWQAFDSI
                     VEPSVTAQGTMAGGELTPPTQIAFSDWGSFADNLWDVNLVHGREFVRARESDLDSAVA
                     MYWSPSLLAPGEVRHYVTYYGLGGITLAPGKLALGMTAPSEVGLSADGTASFLAVAYV
                     SNTGEGPAHDVTASLSLPIGFTLADGHARTRTVGDLEPGETAQVAWQVIASWGAVGGG
                     NLEVNVSATNANPNKVSRRVEILAPPRLLARISHLEALQVRGEEIYPWPVRLTVRVEN
                     TGGSPAHWARVKLDSIGGFSVVGGDVTQLLLGTLEPNRPLDFTWVLGGHPDAGTHTIL
                     VSASADDVPASRASASIQVPLLVPKVWVKPSKTRVMPGDIFSVDVMITNVPDLKSAKF
                     DLRYDNDFVDLALVSRGGLFAAGGELMPWSEGDSRSTPGRVAGTWGSLNSPGYAWGSA
                     ATFYFQARAMGDARFSVENLVLSDASGRVISLESVPTPCTVQITRQ"
     gene            complement(4770..5753)
                     /locus_tag="PLU15_06065"
     CDS             complement(4770..5753)
                     /locus_tag="PLU15_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30522.1"
                     /translation="MIQSRQTQGQLESLLISVLIHAAIIFLVPYVAPAPTVDVYPLDV
                     AGVIEISAIETEPASPAPAPGLNVKEVAPTPASEPPKAAPTKKKDPKPAVAPAPAAKP
                     QPQPAAEPKPVGKTEPKVPTPAVDKPEVVSSPAGKSVAPASEPEPVPAAASAQVPEPN
                     STLSKAVESPTASAKSYTEAKPASGSGTVGGTGEKPPTAGDPGETGVVPRPEYPGDDR
                     TGYGMISSTGGAGHGVVIPKGVQNIKGRGEAKVRIRVTSDGKVDSVSFLIPPPDKAME
                     KPIRNAIMGEWVFKPDTTLGREDAYYLDVWISYDGGTEDISVESERVSYKR"
     gene            complement(5750..6178)
                     /locus_tag="PLU15_06070"
     CDS             complement(5750..6178)
                     /locus_tag="PLU15_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOO30523.1"
                     /translation="MFENHRAKRRLGHVIITPLIDVMFYLVAFLLVFGTFRTETTGMP
                     MELPRAATPSDLAREHIIVAIDSSGRIYYDERIVVDSELTTGLLPRLAANPDRLVIIK
                     ADKSVTYERLIQVIDAIRMAGGVHLALAVERAPQSGGVER"
     gene            complement(6178..6804)
                     /locus_tag="PLU15_06075"
     CDS             complement(6178..6804)
                     /locus_tag="PLU15_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOO30524.1"
                     /translation="MAMLKYIKAGGPVMYPIIFVSIIGLAVVIEKIIQLFHTQTDSDR
                     LMRSVKLNLAQGRPVEAMQAVKNEKGPIAGVARAAIANYDKPREQIRAAVEAAGREEV
                     FMMERKMNVLDGVITLAPLLGLLGTVTGLIKSFQVLSAFGGLTTPAQLSGGIAEAMIT
                     TAAGLIVATPLVIIESWLSVVIDDRVADMEKYAIELIALAAGEDEGDQ"
     gene            complement(6893..8047)
                     /locus_tag="PLU15_06080"
     CDS             complement(6893..8047)
                     /locus_tag="PLU15_06080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO30525.1"
                     /translation="MSKKSADIVVIGGGVVGCATAFELARLGCKDIVLVERNTIASGS
                     TGRCGAGVRQQFGTELNCVLARESVRIFEQMDEYLDYKPGVEFRQSGYLMLAYSDSQW
                     EQFRRNVKLERSLDIDVEMLTPEGAREIVPFLNLEGMVGATFCQTDGHANPFHTAFAY
                     AQAAKRLGAEVLTHTSVVDIEVENNRIKAVVTDKGRIETSAVVNCAGAYSAKIAAMAG
                     IDLPVWGERHQILITEPVEAMMGPMVISFHHDFYCQQTPHGSFIMGIGMPGEPKSFNI
                     HSSVDFLEGMAKTVMKILPPLGNLRVVRQWAGLYDRSPDAQPILGECPPVERFYNANG
                     FSGHGFMVAPIVGRAMAELIMLGKPSVDISKLDAGRFERGELVLEPAVVG"
     gene            complement(8040..8336)
                     /locus_tag="PLU15_06085"
     CDS             complement(8040..8336)
                     /locus_tag="PLU15_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO30526.1"
                     /translation="MTDRPERIDDDVIVCRCEDVTLGDIRRLIANGYHTIDEIKRISR
                     AGMGQCQGRTCRHLIARELATALGVSVEEVEMPTFRPPTRPVLLSTIARGEGDE"
     gene            complement(8333..9427)
                     /locus_tag="PLU15_06090"
     CDS             complement(8333..9427)
                     /locus_tag="PLU15_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO30527.1"
                     /translation="MKEVDIAVIGAGPAGLAAALACAEAGASTLLIDANDVPGGQLVK
                     QTHKFFGSERQHAGTRGIQIPELFAKKLVEHPNVETMWDTTASGYYDSGELVVEDGKR
                     FIKILPKRLIVSTGASERFVLFENNDLPGVYGAGAVQTLMNVYGVVPGDRVLMVGSGN
                     IGLIVSYQLIQAGVDVAAVIEAAPCIGGYLVHAAKLRRAGVPILTSHTIVAAHGEECV
                     TGATIAKVDEKWQATPGTECEIECDVICLAVGLMPLVDILWQAGCEMKYVPELGGHVP
                     VRDDNMRTSNPNVYVAGDSGGVEEASSAMITGEIAGISAAESLGLKAPEADARLAQLK
                     ADLAAIRSGPAGEKICAGVCRATVCGGVWE"
     gene            complement(9459..9770)
                     /locus_tag="PLU15_06095"
     CDS             complement(9459..9770)
                     /locus_tag="PLU15_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO30528.1"
                     /translation="MRLKDHPILDFSAGQEVAFTFDGKKLKGIDGEPIAAALHANGVR
                     VLRHSPVSGRARGFFCAIGNCSSCLMTVDGVPNVRVCTEPLREGMVVETQKGHGQLPI
                     D"
     gene            complement(9879..11597)
                     /gene="recN"
                     /locus_tag="PLU15_06100"
     CDS             complement(9879..11597)
                     /gene="recN"
                     /locus_tag="PLU15_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOO30529.1"
                     /translation="MLSEIDVRNYALIERLSLRFGNGFNVLTGETGAGKSLVIDCVGL
                     VIGGRASSESVRAGCDSAVVEAVFNISDVPDVEALLLEQGIDPEPDGTLIVTREVSRQ
                     GRSRCRLNGRIVTLTALSAIGERLVDIYGQHEYQSLARPSRHIGLLDAFGGARLEQVR
                     DSVRQLYEEWRGCEEELAELRQHARERAQRADLLAFQVNEIESANLEPGEDVHLVAEQ
                     TVLANAESLYDAVVRAYSLIYESSYPDIASARDMLGEALEELDNASRIDGTLTSLAES
                     LSALCDHTTEISRALREYRDSIEFDPERLAEVEERLSAISRLKRKYGDSLADVIDHGQ
                     LAREELESMTGAGRRVDELEGRSAALSEELGNCASKLSAMRCEIAAELEKEVSRELRD
                     LSMPHAVFKVDVGRKRDGHEIVVDGTPVVIGPDGFDDVEFLFSANPGEEPRPLARIAS
                     GGEMSRVMLAIKSVLAVADAMPTMVFDEVDAGIGGEAAIAVASKLANIGKVRQVLAVT
                     HLPQIAAVADNHYAVSKLERSGRMEVTVEKLDIEERVTEVARMLAGEAPSSVTVAHAR
                     EMLSRR"
     gene            complement(11659..12108)
                     /gene="argR"
                     /locus_tag="PLU15_06105"
     CDS             complement(11659..12108)
                     /gene="argR"
                     /locus_tag="PLU15_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOO30530.1"
                     /translation="MKARRHQRILDIVRSQTIKTQEELAEELAREGITVTQATISRDI
                     KELRLIKAPVGDGTYRYTIPTDRSIEDINRRVERIFREVIISIEDSENIIVIKTLEGA
                     AQAVAAVLDDLAWREIVGSLAGDDTILLIIKPVQSVPDVMSRLNKFR"
     gene            complement(12152..12952)
                     /locus_tag="PLU15_06110"
     CDS             complement(12152..12952)
                     /locus_tag="PLU15_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOO30531.1"
                     /translation="MHAAKPEARRLAGHVEEWMRGAGVDVVIDDCLDRPLPEDIDCAI
                     VLGGDGTLLSAARRAATRGIPILGVNLGNLGFLTEVEPSQLEPALRALVDGNYTVEER
                     TMVEAVVFRNGQAVKTLCGLNDVVITKGAFARIVQLGVFVNDAYFATFPADGVIISSP
                     TGSTAYSLSAGGPLINPNIDVLLITPICPHSLFSRSLVIAGSDKVRVVVETAHEDVAV
                     TVDGQIGFELHSMDEIVVGRAPYVTRFVRLETRNFYGILRERLKRDRL"
     gene            complement(13053..13961)
                     /locus_tag="PLU15_06115"
     CDS             complement(13053..13961)
                     /locus_tag="PLU15_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOO30532.1"
                     /translation="MSQQAKIRLDQLVVERGLAASREAGKREILAGNVFVDGVREDKP
                     GTMIRADAKISLERRGPRYASRGGEKLAHALKTFAIDVNGRVAIDIGASTGGFTDVLL
                     KAGASRVYCVDVGYGQLAWSLRQDPRVDVFDRTNARYITPDMFDPRPCFASVDVSFIS
                     LSKILPALYAVLQGRAEAVCLVKPQFEAGRRNVGKRGVVRDPAVHAEVLRAVYGHARD
                     AGFEVLEYTWSPITGPEGNIEFLVHLRKQDANTQRICSCPHAGADAPTSCSLDGELCG
                     ITEAQLDRAIKSTVQQAHVAHGRSGD"
     gene            complement(13945..15864)
                     /gene="dxs"
                     /locus_tag="PLU15_06120"
     CDS             complement(13945..15864)
                     /gene="dxs"
                     /locus_tag="PLU15_06120"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOO30533.1"
                     /translation="MNSILQSIDSPSDLRRLSDREIRQLAEEIRHYLVHTVSRTGGHL
                     APNLGVVELTIAIHLALDCPRDKVIWDVGHQSYVHKLLTGRRGAFASLRQQGGISGFP
                     KPSESEYDAFATGHSSTSISAALGMALARDAAGENYTVMAVIGDGALGGGMALEAMNH
                     AGHTKTDLIVILNDNEMSISRSVGALAGVLGSARTHPGRRRFKYGFDRFVMGIPVIGK
                     ALKAAGYRLKKSVKSFVLPGMVFEELGFTYLGPIDGHNVKAVSRAIDDAKRAGGPVLI
                     HVITRKGKGYAPAEQDPAKFHGTGPFVVETGTQISSDRPTFTSHFSEFMLRAAEEDDR
                     IVAITAAMAEGTGLRSFAEAYPGRFYDVGIAEQHAVTFAAGLAAAGLRPVVAIYSTFL
                     QRAYDQIVHDVALQGLPVILAVDRGGIVGEDGPTHQGLLDVGYLRQIPGMTVMAPKDG
                     AELQSMFGTALEHDGPSAIRYPKSACPEREVEWPARPIPIGKAEVMRDGLHCAIFALG
                     PMVWPALEASRILSTRGVSCAVVNPRFVKPIDVDTLLEYAGRTGIIITCEDSCLAGGF
                     GSAVLESLSEAGVFDVHVVRLGAPDDFVGQGKRDDVLSDLGLDAKGIADAVMQALRGR
                     EGERAVGGGLYVSTS"
     gene            complement(15942..16526)
                     /locus_tag="PLU15_06125"
     CDS             complement(15942..16526)
                     /locus_tag="PLU15_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30534.1"
                     /translation="MPRDFGRIIAVGLWTFALSAALSYLSGSAAGNLSPWFSVAVLMA
                     IIATGILFDIVGTAAAAASEAPINAMAANRVYGASEALRLVRNSPAVASICNDVVGDI
                     CGTVSGAVGATIILRIAASTGGWLAESASVLVIALISAVTVAGKAIGKRVAIESANDV
                     VLSVGRIIATYERFTGRSKDAKGRKRNNGRSSKD"
     gene            complement(17434..17898)
                     /locus_tag="PLU15_06130"
     CDS             complement(17434..17898)
                     /locus_tag="PLU15_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOO30535.1"
                     /translation="MFAPDTGLFSNRILWTSILAWAIAQTLKVFTCIWAEGKPDWSKI
                     VGPGGMPSSHSAFVTSIAVGVGIQEGWNSALFAVTVGFALVVMYDAAGIRRAAGEHAQ
                     VLNQLIERAFREGYLESTKLRELLGHTPLEVAMGAFLGIVVSWLMIGWALKG"
     gene            complement(17909..18799)
                     /locus_tag="PLU15_06135"
     CDS             complement(17909..18799)
                     /locus_tag="PLU15_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO30536.1"
                     /translation="MRLPQWYSEFVEKVDAALDDVMPGGDVELRALHEAMRYSVFPGG
                     KRIRPAMVRASCSAVARVHPSCHTDAVIWPSVALELVHCYSLIHDDLPCMDDDDYRRG
                     KPSCHKAYGEAVALLAGDALLTLAFEVLTSNGFVDVVGPDATCSCVQALASAAGGNGM
                     IGGQVLDISPWPWPGSERVDSGALCRMEMLKTGKLFECAAEMGGIVGGANAEELGVVC
                     GIAADIGLAFQIRDDLEDADSQRDSGRSGFLTAVSVWGIDGARRQFAALMDSARTKCA
                     VLGDAASELTDIIDIVDREW"
     gene            complement(18796..19065)
                     /gene="xseB"
                     /locus_tag="PLU15_06140"
     CDS             complement(18796..19065)
                     /gene="xseB"
                     /locus_tag="PLU15_06140"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOO30537.1"
                     /translation="MSIPSDMTFETALARLEKIVEKMDSGEPSLEEALKLFSEGTELV
                     RYCSSVIEDAEGKLEVLVQGKDGRDVAVPFEEFNSGDEDREDDEA"
     gene            complement(19069..20364)
                     /gene="xseA"
                     /locus_tag="PLU15_06145"
     CDS             complement(19069..20364)
                     /gene="xseA"
                     /locus_tag="PLU15_06145"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOO30538.1"
                     /translation="MAGRYKAGTSGASDGGFGPHGSIGTCGACGSDGIFSASGIPNLP
                     GMPQMAGKVGSYEIFTVSEITRRVARALDRDPVLGDIWVNGEIANFTHHSSGHMYFTL
                     KDDSSRLRAVMFRGSNIGLRFRPADGMRVYAHGYVGVYERGGEYQLYVDLMEPAGLGE
                     LYLAFQQLRAKLEAEGLFDPAAKRPLPKFPRRLGVITSPTGAAVRDIITVTRRRWPGI
                     NILVIPALVQGSDAPASIVQAFDRAGEVPDIDVIIVGRGGGSAEELWAFNDEEVARAI
                     RRSRVPVVSAVGHETDFTIADFAADVRAPTPSAAAELVVPDASAYAHHVELLEARLVR
                     TVRMRVMQRRQYVDELSRRLIQAIQTKLRTNKDRLEYLGRMLDALDPEAVLGRGYAIC
                     RGQDGTVLRRADQVAGGDEVTVQLGCGELGCTIHDVKVG"
     gene            complement(20371..21354)
                     /locus_tag="PLU15_06150"
     CDS             complement(20371..21354)
                     /locus_tag="PLU15_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HOO30539.1"
                     /translation="MKVGTLGFDTSCYTTSVALVISGILAYDGRAPLQVSKGKVGLRQ
                     SEALFQHVRNLPRLVEEACDVARRESCDIVAVGASAWPRRMEGSYMPVFLASSGYGRA
                     VAAALEAPCLEISHQEGHIAAARWSSGLESRGGFLALHLSGGTTELLSVHDRFEMELL
                     GGTGDLNAGQFIDRVGVALGLPFPAGPDLERLAGHGVPGRVRLPVAVSGLKVSFSGPC
                     SAAMRELDSGAGHADMAYAVLECIGRSVGAMCCEGLRFFRAQELLVAGGVASNYIIRR
                     ILSETIEDQLPGAKVYYASPEHSRDNAVGCALLAWQMHLEGMRESREEGKE"
     gene            complement(21329..21886)
                     /gene="nusB"
                     /locus_tag="PLU15_06155"
     CDS             complement(21329..21886)
                     /gene="nusB"
                     /locus_tag="PLU15_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOO30540.1"
                     /translation="MRSERRRAREAAMRALYQMDMSGCTSEDAISYAVSSSSDLDLPE
                     RAQGYASQLVCAAVERMTEIDAVMAGIAHDWAVQRMSPTDRNIMRIAVCEMLYLKDVP
                     VATAIDEAVEIAKLYGTADSPRFVNGILGALAERIKDGQGPADGADAGEGRAESSDAA
                     SSGDVEGDSGGECLGGAHEGRDTRV"
     gene            complement(21903..22133)
                     /locus_tag="PLU15_06160"
     CDS             complement(21903..22133)
                     /locus_tag="PLU15_06160"
                     /inference="COORDINATES: protein motif:HMM:NF021542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HOO30541.1"
                     /translation="MPWFGDGRFNVPEGSSGQFVGAIVGAIFGLLVLIAGFWRALAFA
                     LFVGIGFIVGRYIDVNDEFRERFARRFGRRRN"
     gene            complement(22185..22724)
                     /gene="amaP"
                     /locus_tag="PLU15_06165"
     CDS             complement(22185..22724)
                     /gene="amaP"
                     /locus_tag="PLU15_06165"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOO30542.1"
                     /translation="MKTIDRVILIATAVIIVFLGSVVLASVSGGGFPNAFAIAFYDSV
                     ASNRIAAAIIGIAIVLVTLYVIMMALRVEESAEPIITGTGLGDVQISMAAVEAIAQRA
                     AQKVDGVRNVEAAAEGNEDGITLHARINVYPDVSIPEVCENLERELSEAMHAVIGLPV
                     KSIHTVVKGVSRMESKPRP"
     gene            complement(22763..23146)
                     /locus_tag="PLU15_06170"
     CDS             complement(22763..23146)
                     /locus_tag="PLU15_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOO30543.1"
                     /translation="MELMEDPGMGSVKIANEVVAVIAGLATIEVEGIAGMSGGIAEGW
                     SELWGKKNLARGVKVEVGEQQAAVDLNVVVKYGAVIPEVCEEVQRNVKRTIEIMTGLE
                     VVQVNVHVTNVFIEEKETSTDVRVR"
     gene            complement(23269..23979)
                     /locus_tag="PLU15_06175"
     CDS             complement(23269..23979)
                     /locus_tag="PLU15_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOO30544.1"
                     /translation="MIKKTKQYFGQRGDRFWLKASYLMLAVAVVILGITAIVDPDLYR
                     RRSSAPAVDAEGDNGLSVTEDDGSEEDLSEGEESADLCGATLPTSVLSDGNDQYGSVG
                     GPGSVTPFGAEPFIMPSGADFFEELKIDRDKTRSREREVIQDLIEDPNTHPDVRKRAQ
                     EALLSLAERARREAEAESMIQAKGYEQAVVFLSEAGASVVVKSGRLEEAEVKRIGDAV
                     SAATGVELRRISIMEREP"
     gene            complement(24076..24720)
                     /locus_tag="PLU15_06180"
     CDS             complement(24076..24720)
                     /locus_tag="PLU15_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30545.1"
                     /translation="MSEERRFGTDLLKMPGLKPLLLLAGLGVLLIIVGSLVKTSPRDS
                     GNAVDGYSGLEPISSETSPPSSAVGDRLSDLSPADRYRVDLETRSAAILSKVEGAGTV
                     SVQITLEGGTYREYAVNSVEEYKNVDEKMSSGEARVTTEKRETRDVVLPSDPSSGRGQ
                     SPILVLERSPAISGVLVAASGASDSRTKRELAEAAQTLLGVPAHRVMVVVAKSP"
     gene            complement(24717..>24977)
                     /locus_tag="PLU15_06185"
     CDS             complement(24717..>24977)
                     /locus_tag="PLU15_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30546.1"
                     /translation="AELTETVIEAKVREALAARFYGKNCFWEVRASIDRSGALLGIEV
                     NVSFGGAAEGCARPGDSEIAAAASAALGVDPEMIIVRQVGRR"
     assembly_gap    24978..25077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25078..25301)
                     /locus_tag="PLU15_06190"
     CDS             complement(<25078..25301)
                     /locus_tag="PLU15_06190"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HOO30547.1"
                     /translation="MEAISAWVRGIVVLVLAVTFLEMLVPRSGLKKFVDMVMGLAVIA
                     GIIAPLVNIGMGYVDGKLAEADRFAMSGNLG"
     gene            complement(25452..26858)
                     /gene="spoIIIAE"
                     /locus_tag="PLU15_06195"
     CDS             complement(25452..26858)
                     /gene="spoIIIAE"
                     /locus_tag="PLU15_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HOO30548.1"
                     /translation="MMRLGLVKLPLVQVGGGGLAEEAIAGRTPGSAWIAGTVRMVKKG
                     KTSVRSGGAGQVGTGPKGMRERSSCSPAKVAVIMICFILCSHWAVAKCSGRGESGIAD
                     SPDSLLAEAAMEQISAMDVAEIERFIRGLDDDIAENMPEFNIIEIMSNQAAGIFDLGG
                     LARKAVSFLLREVKVNLKLLGELIVVAMIGSILCTFGSGLGEEGPCAMAWAVCYMTLV
                     TMGIRSFSVSADLATGAVDSMISFMHAIIPALVGLLATSGGVTTAAVVSPLVLVATTT
                     VGTLVKTTVIPLLLLAAALTLVGNVSDRKQVTKLAGLLRWWSMTILGLASTLFVAFVG
                     VRGGLASVSDAASAKAAKFLAGSMIPVIGKVFGDAIDLVAASSLVVRGALGAFGLVSL
                     VVVCLFPVFKMVAVMLMFKIAAAVAEPLGDPRVSDCLGELADVLSALCICVTTVGLMF
                     FICISLVVGLSGLVVAIR"
     gene            complement(26855..>27231)
                     /gene="spoIIIAD"
                     /locus_tag="PLU15_06200"
     CDS             complement(26855..>27231)
                     /gene="spoIIIAD"
                     /locus_tag="PLU15_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HOO30549.1"
                     /translation="VVGMAILGAVLLAFLRQTRSDMAIVLSAALGIVVFLMVVTRLAY
                     FVEVLSDLAVRAGVNRMYLDTVLRVIGVAYLAAFGGQVCRDAGEGALATKVELAGKVL
                     ILAMSVPLMFALLDLILRILPS"
BASE COUNT         6055 a         8671 c         6489 g         5916 t
ORIGIN      
        1 tttcatcata gtaccttgcc ccgttgctgc aactgcctca tagcagttgt ggcaaggata
       61 tacggcaacc cgcagaacgg agttggaagg ttgccatgag aagtttgggg acagggatta
      121 cagcttaacg cgagaatcat gcaccattgc catgatcgat accaagacct gaagagacaa
      181 tcttcgttct ttgatcaaaa gcaggccggg gcctcaacga cccccgcctg cttataagct
      241 acatgctacg tataccgcct caaagaaaag gcaaaacgct tccaaaagga gcattagagc
      301 gctcgagagt ttcctacttg cccccgcttc ctatagctat tgcagctaca gcggcaataa
      361 cgccgagtac tgcaacgatg gctccggcag aaccgggctt tttcgcatct gcctgggcct
      421 tctcaccctc aagtttttcc accctcgcct caagctcttc aatcatcgat tgaagcttga
      481 cctctctcat ctcggcttcc catctttcca ccacaaggtc atccgagaac cttttggcta
      541 gctcgtccac acttcctgca agcaacctgt ctatgcttgc tagatgtgaa tctatctcgg
      601 cgagcctagc agttatctcc tctatcgagc cttcggcctg acgaactgct atatcggtct
      661 gccccaaacg ttcattcgta gcatcaagac tcctcgccac atcctgaatg cgcatggtgt
      721 tctgaagcac ccttccgttg gtcttcaggt cttctgcggc attggccttg atggccgccg
      781 acagatcggc agatgtctgg tccaaggcct tcgccaactc cgccagggca gcttgctgtg
      841 acgcataatc ctcgctggtc ttttcagaca tctggagcag ctccgcctcg atgcctgcgg
      901 catactcctg gagggtcctc acatctgcag cgagacgtgc aagttctgca ccgagttcgg
      961 cgactcggct ctcctggatc tccagggccg caatgatggc agcgtctgct tctgcagtct
     1021 ttctttctat gtcagtaatg tcgcgccggg cggccgcaat ttcatccaga atccttcttg
     1081 cctcttcctg gtgggcaata tctgccgctt cacgctgcgc gcgctcggtc tcgagttcct
     1141 tgcccaagga aagtgaaaag gcatctatct tgctcgagag ttccgcataa gccgccttcc
     1201 tgtcggcgtt ttcgccttcg atcgcactgt cgattttgcg agttagcgca gattcaagct
     1261 gagcttggcg ggccaacata tcgaggccca gagcctctcg cgctttggac tcttcatcca
     1321 aggacaaaga caaggcgctt acctgttgcg ccagtcctgc ctgcgccttt tccctatcgg
     1381 ccttttcacg ttctatcccc gcagccaatc tgagagcagt tgtctgctcc aattcgtcct
     1441 gggacgatgt taaagactcc agcctcgcca tgagttcggc aagttcggaa gctcgcgtgg
     1501 cacgctcagt ctggatcgcg ctgtctatct tgagattgag cgtcgattcg agctcagttt
     1561 ggcgggccgc aatctccgca tccgaagcct tccgctcttg agtctccttg ctcagagaaa
     1621 gcgacagagc atctatctgt tgcaccagtc ctgcatctgc cttttcccta gcaacacgtt
     1681 cgccctctat ggcagatgaa agctgtccgt tctgatatgc ctgcatcgac ccgagcttca
     1741 gctcagtgtc gacgcgctga gcatccaaag cgtctgacag ctggctgacc ttgttatcaa
     1801 gcgctgccaa tgcgctctcc atccaggcct tattcgctgc caagccttta gctgtgtcat
     1861 caagcctgag agagagcgtg gctatgcgtg atcccgtctc cgactgaagc gcgttcaatc
     1921 ttccgtcaac gtcatttacc cgcgccccga gctcattcat ctgggcaaca taagcccgct
     1981 ggtcctcgct gatcttattc agagatagcg tcagcttggc ctcgagatca gcacgagact
     2041 gggcctcaga agcaaacgac ctatgtgcaa aagcctccaa ggagtcggct ctggccgcaa
     2101 gctccgaaat ctcctggccc agctctgttt cgatctcttg actctgtttc ttcagatcac
     2161 cgaagaccgc agtaagcttc tgatcggacg cacccgcggt ctttccgatc tccgccagca
     2221 attgcttgcg ctgctcctcc atggcctgtt ccaaccggct gtagttgagc gccactgaag
     2281 cagaagtctc ctgcgcgatt ttggcgtcgc ctgccgcacc ggcggcaatc cgctctacct
     2341 cctccagcct ggcagtgacc acagccaact gctccctgag gaccgtggct gtctccctaa
     2401 ccactctcat gtcgtcctcg ctcagagttg tacggcccgc ctccacatca cggaggagtt
     2461 ttgccgtcac aaaggcaagc aaatacctgc tgaccggatc atctccacag aaattgccgc
     2521 tctcatatac gcccatatat cccttttcta caagaagctt cactgcttcg tatgcccaat
     2581 gatcctctgg cacgtccttt attgtgaccg gcgccgccag gactgctgag gacattagcg
     2641 agatcactat cagcacactt atcgctgtga tgctcagccg cttcgaaatc tcagacatca
     2701 tgaccccccc atttgaccgt tgatgttatt gcctggtgat ctgaaccgtg cagggagtag
     2761 ggacggattc cagcgatatc accctgcctg acgcgtccga aagaacgaga ttttctacgg
     2821 aaaatcgcgc atctcccatt gctctcgcct ggaagtagaa tgttgccgcg ctgccccaag
     2881 catacccagg cgaattcaag gagccccacg tccctgcgac tcggcccggc gtcgatcggg
     2941 aatctccctc gctccacggc atgagctcac cgcctgcagc aaatagtccg cctcttgaca
     3001 cgagggccag atcgacgaag tcattatcgt acctcaaatc gaacttagca gatttcagat
     3061 cagggacatt ggttatcata acgtccacac tgaatatgtc gccaggcatt acccgtgttt
     3121 tgctgggctt cacccaaacc tttggcacga gcaaaggcac ctgaatagat gcgctagccc
     3181 tagacgccgg cacatcatca gcagaagcgc tcaccagaat ggtgtgggtg cctgcatcgg
     3241 gatggccccc cagaacccaa gtgaagtcga gtgggcgatt gggttccaac gtcccaagca
     3301 gcaactgcgt tacatcgcct ccgacaacgg agaagccccc aatcgagtcc agcttgaccc
     3361 tggcccaatg agccggcgat cctccggtgt tctccacacg aaccgtaagc ctcacgggcc
     3421 acgggtagat ctcctccccg cgcacttgga gggcttccaa atggcttatc ctcgccaaca
     3481 ggcgcggagg cgccagtatc tcgacacgcc ttgaaacctt gttcgggttg gcgttggtcg
     3541 cgctaacatt gacctctagg ttgccgccgc caacagcacc ccatgaggca atcacttgcc
     3601 atgctacctg tgcggtctct cccggctcaa gatcgccaac cgttcgggtc ctggcatgtc
     3661 catctgccag ggtaaacccg attggaagcg acagagaagc agtcacatca tgagccggcc
     3721 cttccccggt attgcttacg taggccactg cgaggaacga ggccgtcccg tcagcagaaa
     3781 gccctacttc ggacggtgct gtcattccca gagccagctt gccaggggcc agggtaatcc
     3841 cgcccaagcc ataatatgtc acataatgcc tcacttcccc gggtgctaga agcgatggcg
     3901 accagtacat cgcgaccgca gaatcgaggt cagactctct ggcgcgcaca aattccctgc
     3961 cgtgaacgag attgacgtcc cataaattgt cggcaaacga accccaatcg gagaaagcta
     4021 tctgagttgg aggcgtgagc tcgcccccag ccattgttcc ctgcgcagta acagaaggct
     4081 cgacaatgga atcaaaggct tgccaataat caggtatctc tcttcttgaa agcatagtat
     4141 ctgaaacgat cgtcttttca ccgacacgga gtggcgctcc gtcgttggct ccgagcatcg
     4201 tgtcgatcat caaccgcaag cccacactcc gcgtcacgct ccccgtattc tccagcttgt
     4261 agtggatcct cgcagtatcg aggtaacgcg tgctcggact ctttacaaat gaaagttcct
     4321 gggtgacaag aatatctccc agcatgtagc tgcagacgac cttgccgtcc tctatctgag
     4381 gaggccgtac ctgttcgcca tacatacctg atcttccagc gcgacgagtc gttttcccgc
     4441 cgaagaccca atctgagccg tcgatgcgaa ccgtcgtata ggaagtccac ggcatagggc
     4501 gtccgtaaat aagcggcagt ccatcgtcgg ctgggtttcg gggatcgccc ccagtggtct
     4561 ccaatccaaa cctacccgcc tctgcgggtc cgggattcac ggtgatcctg atgaattgat
     4621 tgcttatctc aacaggcgcc gcagtgtccg ctcgtgagat tcccggatgc atgataaatg
     4681 cacaccagag aaggacaaga gctgtgacga ccgcgcgcgc cttgcatgat cttaccatga
     4741 tataaggcac ctccgcgtgc gttcaacaat caccttttat acgaaacgcg ttcgctttcg
     4801 accgatatat cctccgttcc gccatcatag ctgatccata catcgaggta ataggcgtct
     4861 tcccgtccca aagttgtgtc tggtttgaaa acccattccc ccataatcgc gttgcggata
     4921 ggtttttcca tagccttatc cggcggcgga atcaagaaac taactgagtc gacctttccg
     4981 tccgatgtca cgcgtatcct aacttttgct tctccccgac ccttgatgtt ctgcacgcct
     5041 ttgggtataa ccacgccgtg accggcgccg cctgtagaac ttatcattcc gtatcccgta
     5101 cgatcgtcac ctgggtattc cggccgcgga actactccgg tttctccagg atctcctgca
     5161 gtcggaggtt tttcacccgt tccgccgaca gtgcctgatc ccgaggcagg cttcgcttcc
     5221 gtatagctct tggccgatgc agtcggcgac tcgacagcct tgctcaatgt cgaattcggt
     5281 tctggaacct gtgcggacgc ggcagcggga acaggttcgg gttccgaagc cggggcaacg
     5341 ctctttcctg ctggactcga aacaacttcg ggcttgtcaa ctgcgggcgt tgggaccttc
     5401 ggttcggtct tcccgactgg tttcggctca gccgctggct gaggctgtgg tttggcggca
     5461 ggcgctggtg ctaccgcagg tttaggatct ttcttcttcg tcggcgctgc ctttggaggc
     5521 tccgacgccg gcgttggcgc aacttccttc acattgagcc ctggcgcagg tgccggggag
     5581 gcaggctctg tttcaattgc ggatatctct atgacccccg caacgtcgag cggatacaca
     5641 tccacggttg gcgcaggcgc cacgtacggc accaggaata tgatcgccgc atggatgagc
     5701 accgatatca gcaagctctc caactgacct tgagtctgcc gactctggat catctctcaa
     5761 caccgcccga ttgcggagca cgttcgacgg caagagccag gtggactccg ccggccatcc
     5821 tgatcgcgtc gattacctga atgagtcttt cataagtgac tgacttgtcc gcctttatga
     5881 tgacgaggcg atcagggttc gcagctagtc tcggcaatag cccggtggta agctcgctgt
     5941 cgaccacaat ccgttcgtca tagtagattc gcccgctcga atcgatagcc acgatgatgt
     6001 gttcgcgggc aagatcgctc ggagttgcag cccttggaag ctccataggc attccagtgg
     6061 tctccgttcg gaatgtgccg aaaaccagca agaaagcgac tagatagaac atgacgtcga
     6121 tgagcggtgt tattatcaca tggcctaacc ttctctttgc acggtgattc tcgaacatca
     6181 ctggtccccc tcatcttcgc ctgccgccaa cgctatgagc tcaatggcgt acttctccat
     6241 gtctgccact ctgtcgtcta tcaccactga gagccaactt tcaatgataa ccagaggcgt
     6301 ggcaacgatg agccctgcag cagtcgtgat catcgcttcc gcgatgccgc ccgaaagctg
     6361 tgccggcgta gtgaggcccc cgaacgccga caggacctga aagctcttga tgaggccagt
     6421 aacggttccg agaaggccga gaagaggtgc aagcgtgatg acaccgtcga gcacgttcat
     6481 cttccgctcc atcatgaata cctcttcccg gcctgcggct tctaccgccg ccctgatctg
     6541 ttcacggggt ttatcgtaat ttgctatagc ggcgcgggca acgcccgcga taggcccttt
     6601 ttcattcttt acggcctgca ttgcctctac ggggcgtccc tgtgcaaggt tgagtttgac
     6661 cgagcgcatg aggcgatccg agtctgtttg tgtatggaaa agttggatga tcttctcgat
     6721 gacgacggct agtccgatta ttgacacaaa aatgattggg tacatgactg gtccgccggc
     6781 ttttatgtat tttagcatcg ccatagctcc ccctctaccg acaaatgcgc ccgcttgtga
     6841 tgtttggacg ctcctccatt cttctacgtt atctggggaa attcctctag gtttatccga
     6901 ctaccgccgg ttccaaaacg agttcgcctc gttcaaatct tccagcatct aatttgctta
     6961 tatcgacaga aggtttgccg agcatgataa gctctgccat agcccgccca acgatgggcg
     7021 caaccatgaa accgtgcccc gaaaaaccat tggcattgta aaaacgctcc acaggcggac
     7081 actcgcccaa tatcggttga gcatctggag acctgtcata aaggcccgcc cactggcgga
     7141 caaccctgag gttgcctagg gggggcagga tcttcattac agtctttgcc atcccttcga
     7201 gaaaatccac ggatgagtgt atgttgaagc tcttaggctc ccccggcatg cctattccca
     7261 tgatgaaact tccgtggggt gtctgctgac agtagaaatc atggtggaaa gagatgacca
     7321 tcggtcccat catagcctca accggctccg ttattaggat ctgatgccgc tcgccccata
     7381 ccggcaaatc gatgcctgcc attgcggcta tcttagcaga atatgcccca gcgcagttga
     7441 caactgctga cgtttcgatc ctccctttat cggtcacaac cgcctttatg cggttgtttt
     7501 ccacctctat gtcgaccaca gaagtgtgag taagaacctc tgcaccgagc ctctttgcag
     7561 cctgcgcata tgcgaacgct gtatggaacg gattggcgtg cccgtccgtc tggcagaacg
     7621 tagctccgac cataccctca aggttaagga aaggaactat ctccctcgcg ccttcaggcg
     7681 tgagcatctc cacgtcaatg tccaagctac gctcgagttt tacattgcgt ctgaactgct
     7741 cccactggga atccgaataa gcgagcatca aatagccgct ctgcctgaac tcaacacctg
     7801 gcttgtaatc gagatactcg tccatctgtt cgaagatcct gacagactcg cgagccagca
     7861 cgcagttgag ttccgtccca aactgctgcc ttacacccgc gccgcatctc cctgttgacc
     7921 ctgaagcaat cgtgttccgc tctacaagca ctatgtcttt gcaacccaag cgagcgagct
     7981 caaaggctgt tgcgcacccg accaccccgc cgcctattac gacgatatca gcgctctttt
     8041 tactcatcgc cttcgcccct tgcaattgtg gatagtaaca caggcctcgt aggcggcctg
     8101 aaagtcggca tctccacttc ctccactgac acgccaagag ccgtagcaag ctctcgcgca
     8161 ataagatgcc tgcacgttct gccctggcat tggcccatgc ctgcgcggga aatgcgcttg
     8221 atctcatcta ttgtgtgata accatttgct atcagacgac gaatatcgcc caaggtaacg
     8281 tcctcgcacc tgcatactat cacgtcgtca tctatccgtt ccggtcggtc ggtcattccc
     8341 aaacacctcc gcacaccgtc gcacgacata ctccggcaca gatcttttcg ccagccggtc
     8401 ccgatctgat ggctgcaaga tccgccttga gttgtgcgag cctcgcgtct gcttccggcg
     8461 ccttaagtcc gagggattca gccgcgctta tgccggctat ctcgccggtt atcatcgccg
     8521 aactggcctc ttcgactcct cccgaatcgc ctgcaacata tacattagga tttgaagtgc
     8581 gcatgttatc gtcgcgaaca ggcacgtgtc cgcccaactc cggcacgtac ttcatctcgc
     8641 accctgcctg ccacaggata tcaaccaagg gcatcagccc cacggctagg catatcacat
     8701 cacattctat ctcgcactca gtgcctggag ttgcttgcca tttttcgtct accttcgcaa
     8761 tagtggcgcc tgtcacacac tcttcgccgt gagctgccac aatggtgtgg gaagtaagta
     8821 ttggcactcc agctctgcgc aacttcgccg cgtgaactag gtagcctcct atgcacggcg
     8881 cggcctcgat cacggccgcc acgtccacac cagcctggat aagctgatag ctgacgataa
     8941 gtccgatatt gcccgaaccc accatcaaca cccggtcgcc gggaacgact ccataaacgt
     9001 tcatcagagt ctgaaccgcg ccggcgccgt acactccggg caagtcattg ttctcgaaaa
     9061 ggacgaatct ctcggaagcg cctgtcgaca ctatcaagcg ctttggcaga atcttaatga
     9121 atctctttcc atcctccacc acgagctctc cggaatcata atagcccgat gcggtcgtat
     9181 cccacatagt ctctacattg ggatgttcca cgagcttctt tgcgaaaagc tccggaatct
     9241 gaatccccct tgttccagcg tgctgccgtt ctgatccaaa gaacttatga gtctgcttta
     9301 caagctgacc ccctgggaca tcatttgcgt caatgagcag agtagacgca cccgcttcgg
     9361 cgcacgccag tgcagctgca agccccgctg ggcctgcgcc aatcacagca atgtcaactt
     9421 ccttcaacag gttcaccgtc cttgctgctc ggcccgcctc agtcgatggg cagctggccg
     9481 tggcctttct gagtttcgac taccattccc tctctcagcg gttctgtgca cacacgcacg
     9541 ttcggcacgc catctacagt catcaagcat gacgagcaat tgcctatagc acagaaaaac
     9601 ccccgtgcac gtccactaac tggactgtgt cggaggacac gaacaccatt ggcatggaga
     9661 gcggcagcta ttggctcccc atcaatccct tttaactttt tcccgtcgaa agtaaatgca
     9721 acctcctgtc cggcagaaaa gtccagtatg ggatggtcct tcaatctcac cttgctcggc
     9781 cgtaggccac ctgtgaagcg gcccaagccc tccctcctcc tgacacaaaa tgctttcttt
     9841 cgcaattctt caacataggc atgccaactc cttcatccct agcgccgaga aagcatctcc
     9901 cgggcatgtg ccacggtcac agatgatgga gcctcgccag ccagcatcct cgctacctcc
     9961 gtcaccctct cctcgatatc gagcttttcc actgtcacct ccatcctccc actcctttcc
    10021 agcttggaaa cagcgtaatg attatcggcc accgcggcaa tttgcggcaa atgggttacc
    10081 gcaagcacct gccgaacttt gcctatattc gcaagctttg acgcgacagc tatcgccgcc
    10141 tcgcccccga ttcccgcatc cacttcgtcg aagaccatgg tcggcatggc atccgctaca
    10201 gctagcacag acttgatcgc caacataacc cgggacatct ctcctccgct ggctatacgc
    10261 gcaagaggcc tcggttcctc tcccgggtta gctgaaaaca gaaattccac atcgtcaaag
    10321 ccgtcaggac cgattacaac gggcgtgcca tccacaacta tctcgtgtcc atcccttttt
    10381 ctgcctacat caactttaaa gactgcatgg ggcatggaca ggtcccgcag ctccctggag
    10441 acctccttct caagctcggc cgctatttcg catctcatcg cagagagctt ggaggcgcag
    10501 ttgcccaact cctccgagag ggccgctgaa cgaccttcca gctcatccac gcgacgccct
    10561 gctcctgtca tggactccag ctcctcacgg gcaagttgcc cgtggtctat aacatctgcc
    10621 agcgaatcac cgtacttccg tttcaacctc gaaattgcgg aaagcctctc ttccacctcg
    10681 gcaagtcttt ccgggtcgaa ttcgattgag tcgcggtatt ctctgagggc acgggatatc
    10741 tctgtggtgt ggtcgcacaa tgcagacaag ctctcggcaa gagaagtcaa tgtcccgtcg
    10801 attcgagacg cattgtccag ttcctcgaga gcctcgccca acatgtcgcg ggctgatgcg
    10861 atatcaggat aagaggattc atagatgagg ctgtaagccc gaacgacggc gtcgtaaagg
    10921 ctttcagcgt tggctagtac tgtctgctcc gcgacgaggt gaacatcctc gccgggctcg
    10981 agattggcag attctatttc attgacttga aaggcgagaa gatcggcgcg ctgtgcgcgc
    11041 tcccgagcat gctggcgcag ctcggccaat tcctcctcgc atccgcgcca ttcttcataa
    11101 agctggcgga ctgaatcccg aacctgctcc aaccgggctc cgccaaaggc gtcgagcagg
    11161 ccgatatgcc gggatggtct ggcgagagat tggtattcat gttgcccata tatgtcgacc
    11221 aagcgctcgc ctatggccga aagcgcggtc aaggtcacaa tgcggccatt cagcctgcaa
    11281 cgtgaacgcc cctgcctaga aacttcccga gtcacgatga gcgtcccatc aggctcagga
    11341 tctatgcctt gttcgagcag gagcgcttcc acatcgggca catccgatat attgaaaaca
    11401 gcctccacca cagcagaatc gcagccggca cggacagatt cagaggaagc ccttccacca
    11461 ataacaaggc ccacacagtc tataaccagc gattttccgg cgccggtctc ccctgtgaga
    11521 acattgaatc catttccgaa tctcagtgac agccgttcta taagcgcgta gttccgcaca
    11581 tctatttccg aaagcatcgc cttgccccca aagacgcggc gaccagcaac agcctgctca
    11641 cacaaccggc ctgccctatt atcggaactt gttcagccgg gacatcacat cgggcacgga
    11701 ctgaaccggt ttgataatga gcaatattgt gtcgtcgccc gccaaagacc ctactatttc
    11761 ccgccatgcc aggtcatcca ggaccgcggc aaccgcctga gcagcgcctt ccaatgtttt
    11821 tatgactata atgttttcgc tgtcttcaat gcttattata acttcgcgga agatgcgttc
    11881 tacccgcctg tttatatctt ctatgctgcg atcggtcggg atcgtgtacc gataagtacc
    11941 gtcgcccaca ggcgctttta tcagccggag ttcttttatg tcgcgcgaga ttgtggcctg
    12001 cgtaacagta ataccctctc gcgcaagttc ctcagccaat tcctcctgag tctttatcgt
    12061 ttgcgatctg acaatatcca gaatcctctg gtgtcttctt gctttcacct ccgacgcctc
    12121 ccgcacatac cttttcccct gcggcccgcc attacaagcg gtcccgcttg agcctttccc
    12181 tcagtatccc gtaaaaattc ctggtctcaa gcctgacaaa cctcgtcacg tacggagccc
    12241 tgccaaccac tatttcgtcc attgagtgga gttcgaaccc gatctgaccg tcgacggtaa
    12301 ccgccacgtc ttcatgggcc gtctccacca caacccgcac cttatcgctc cctgcaatca
    12361 caagagaacg cgagaaaagg ctgtgcggac agattggagt gatgagaaga acatcgatgt
    12421 tgggattgat cagaggtccg cctgccgata gtgagtaggc agtggatcct gtagggctcg
    12481 atatgataac cccgtccgcg gggaaagtgg cgaaatatgc atcattcacg aataccccaa
    12541 gctgaactat tctggcaaaa gcccccttgg taatgaccac gtcgttaagg ccgcaaagag
    12601 tcttgacagc ctgtccattg cggaaaacga cagcctcaac cattgttcgc tcttcgacgg
    12661 tgtagtttcc atcaacaaga gctcgaaggg ccggttcgag ctgcgatggt tccacctccg
    12721 tgagaaaacc aaggttcccc agattaaccc ctaaaatcgg aattcccctg gttgcagccc
    12781 gcctagcagc agagagaagg gttccatctc ctccaaggac aatagcgcaa tcgatatctt
    12841 ccggaagcgg tcgatccaga caatcgtcga tgaccacgtc gacccctgcc ccacgcatcc
    12901 actcctctac gtggccagcc agccttcttg cctccggttt tgccgcgtgc actacgattc
    12961 ccgccctctt cgtaagaccc cccccttttc atcagtccag acccgaagcc ctatctacag
    13021 ccaatgaccg cgcggacacg ttgctgttgg ggctagtcgc cgcttcggcc gtgggccacg
    13081 tgcgcctgct gaacagttga tttgattgcc ctatcgagct gagcttcggt tattccgcac
    13141 aattccccat cgagactgca agaagtcggt gcgtcggcgc ctgcgtgggg acaactgcag
    13201 atccgctgcg tgttcgcgtc ttgtttgcgc agatgaacca aaaactcgat gtttccctca
    13261 ggtccagtaa taggagacca cgtgtactcc aacacctcaa aacctgcatc acgtgcatgc
    13321 ccataaaccg cccgaagaac ttcagcatgc accgcagggt cacgaactac gcctcgcttg
    13381 ccgacgttgc ggcgccccgc ctcgaactgg ggcttcacca agcacacagc ctcggcccgt
    13441 ccttgcagca ccgcatacaa ggcgggcagt attttggaca aagatatgaa ggacacgtct
    13501 acagaggcaa aacaggggcg cggatcgaac atatcaggcg ttatgtagcg cgcgttcgtg
    13561 cgatcaaaga cgtccacgcg cgggtcctgt ctcaggctcc aggcaagttg cccatagccc
    13621 acatccacac aataaactcg cgacgccccc gctttcaaga gcacgtcggt aaatccgccc
    13681 gtagaggcgc ctatatctat ggcgacgcgg ccatttacat cgatagcgaa agttttcagc
    13741 gcgtgcgcca gcttctctcc gccccgactc gcgtaccgcg ggccacgccg ttccagggat
    13801 atcttggcgt cagcccgaat catcgtcccc ggcttgtcct cgcgcactcc gtccacaaag
    13861 acgtttccgg ctagtatttc acgctttcct gcctctcggc ttgctgccag cccacgctct
    13921 acaactagct gatcgagtct gattttagct tgttgagaca tacaaaccac cgccaaccgc
    13981 cctctcgccc tctcgccctc ttagcgcctg catcaccgca tcagctatgc ctttggcatc
    14041 caaaccgaga tcagacaaga catcatcccg ttttccctgg cccacgaagt catctggcgc
    14101 tccgagcctc acaacatgca catcgaaaac gcccgcctct gacagcgact ccagaactgc
    14161 tgaaccaaac ccacccgcca gacaagagtc ctcgcatgtg ataatgattc ccgtcctgcc
    14221 agcgtattcc agcaatgtat caacatcgat cggcttcaca aacctgggat tcaccactgc
    14281 acaacttact ccacgggtcg acagaatcct gctagcttcg agagcaggcc ataccatggg
    14341 cccaagggcg aatatggcac aatgcaagcc gtcccgcatg acttccgcct tgccaatggg
    14401 aataggcctt gcaggccact cgacctccct ctccgggcaa gccgacttag gataccttat
    14461 ggccgatggc ccatcatgtt ccagagcagt cccgaacatg ctctgcagct ccgcaccatc
    14521 cttgggcgcc atcacagtca tcccgggtat ctgcctcaga tatcctacat caagcaggcc
    14581 ttgatgagtg gggccgtctt cgcccactat ccctccgcgg tcaacagcaa gaattaccgg
    14641 cagaccttgc aatgccacat cgtgcactat ctggtcatag gcccgctgaa gaaaggtgga
    14701 atatatggcc acaacgggac ggagaccagc tgcagcaagc ccagcagcaa aagtgaccgc
    14761 gtgctgctca gcaatgccca catcgtaaaa gcgcccggga taggcctccg caaaactgcg
    14821 cagaccagtg ccctcggcca tggcagccgt tatcgccaca atcctgtcat cttcttcagc
    14881 agcgcgaagc atgaattcgc tgaagtgcga cgtaaaagtg ggccggtcac tggaaatctg
    14941 agttccggtc tcaacaacga acggaccggt gccatggaat ttcgcggggt cctgctcagc
    15001 aggcgcatag cccttgcctt ttcttgttat gacgtgtatc aagaccggcc cacccgcgcg
    15061 cttggcgtcg tctatggcgc ggctcacggc ctttacattg tgtccatcga ttggccccag
    15121 ataagtgaag cctagctcct caaagaccat tccaggcagg acaaagctct tgacgctttt
    15181 cttcaacctg tatccagctg ctttgagtgc cttcccaatt accggtatgc ccatgacaaa
    15241 ccggtcaaat ccatacttga agcgtcgtct gcccggatgg gttcgagcgg agcccaacac
    15301 ccctgccagc gctccgacac ttcgggaaat ggacatctca ttatcattga gtatgactat
    15361 gagatcggtc ttggtgtgcc cggcatgatt catcgcttca agagccatcc cgccgccgag
    15421 cgcgccgtct ccaattaccg ccattacagt atagttttca cctgcagcat cacgcgccaa
    15481 ggccatgccg agggcggcag atatggacgt actggagtgt ccggtggcaa aggcgtcgta
    15541 ttcgctctct gacggtttcg ggaaaccgct tatgcccccc tgctgccgca gagaggcaaa
    15601 agccccacgc cttccggtga gaagcttgtg aacatatgac tggtggccta catcccatat
    15661 caccttgtcg cgaggacaat cgagagcaag gtgaattgct atcgtaagct caactacacc
    15721 caggtttggc gccaagtgcc cgccggtgcg ggaaacagta tgtaccaggt aatgccgaat
    15781 ctcctccgca agctgccgta tctccctgtc tgagagcctt cgcaggtcgg aaggtgaatc
    15841 gatgctctgg agaatgctgt tcatagggaa cccaccgtcc cttgaccgtc cgctccacat
    15901 ttgggaggat cggcaataat cattgatgct tcagcgtgcc gtcaatcctt gctacttctt
    15961 ccattgttcc gctttctgcc tttggcatcc ttggaccgtc cagtaaacct ttcgtatgtc
    16021 gcaattatcc gccccactga caacacgaca tcgttggctg attctatcgc tacacgcttg
    16081 ccaatggcct tgcctgcaac tgtgacagcc gaaatcagag cgatgaccaa cacagatgcg
    16141 gattctgcaa gccatccccc ggtcgaagca gctatgcgca gaatgatcgt agcgccgacc
    16201 gctccgctca cagtgccaca gatgtcgccg acaacgtcat tgcagatact agcaactgcc
    16261 ggagagttcc ggacgagcct tagcgcctca cttgctccgt acacacgatt agcggccata
    16321 gcgttgatcg gagcttcgct ggccgcggct gcagcagtcc ccacaatgtc gaagagaatc
    16381 ccagtggcaa ttattgccat caaaacagcg accgaaaacc aggggctgag gttcccagcc
    16441 gcagagccag aaaggtagct caaagccgcc gatagggcga atgtccatag ccctactgct
    16501 atgattcgcc caaaatcacg tggcaagtca tgttcacctc aaaaaatatg ggcttcagcc
    16561 tgaatgcgca taatatggat ccgaaatagt cggatgaata aataaaccga taggtggcag
    16621 cgccctgagt aaatattgtg acttgaggtc cagcaggttt tcccccctgt gcgccctacc
    16681 gtcgccggcg ggcggtttcc ctttacgaca ggttctcctc gtcgccgaag gagcaactgg
    16741 attgccactt agatcacacc gtaatcccca gagcgccatg caatgcaaac agcctaggag
    16801 ttttcctcgg caatgctggc cattctctat cctcttttgc agatgggctt tcaagcggca
    16861 aaaggtcatg ttccttggcc ggggagacaa tgacttggca cccgccatcc cgcacatcca
    16921 ctcaaggcag gctacgctgc acccagcacc attgggccca ccagggccag taccgacatg
    16981 caggttcacc ccaagccgta aagcttttca accccacgaa cttgggtctc cacggaagca
    17041 gggtcaacgc ccacatgcca ttgcggatcg ccccacaacc ttaaccccct gcaccaaccc
    17101 ccaactgggc gtcaaaagcc agcacaaggg acttcatcga tgtgccctta acggtatttt
    17161 atccccgtct tcgagaaagc caacaccgac taggatactg cgccacctat cctgttcgat
    17221 caacattata ccgcaaaacc tagacccatt caatggcagc tgcgcgagcg tgtcggtcgg
    17281 gagctgtcag tcatccatat ggctgacaat gccaggatcg acctggcttg aacggctcaa
    17341 cacttccggc aacgcaggtt ccaagccaca gagatttctc gacagacttc tatatcaaac
    17401 ggatcgagcg aagcgtgcat cgtccaaaaa cggctagcct ttcaatgccc atcctatcat
    17461 gagccaagac accacaatcc cgaggaaggc gcccattgcc acttcgagcg gtgtgtgtcc
    17521 gagaagctcc cgtaacttcg tagactccag atacccctcc cgaaacgccc tctcgataag
    17581 ctggtttagg acttgcgcat gctccccggc agcccgcctg atacctgcgg catcgtacat
    17641 cacgacaagg gcaaatccca ccgtcaccgc gaacagagct gagttccatc cttcctggat
    17701 ccccactccc actgcgatac ttgtaacgaa cgccgaatgg gaactgggca tgcctcccgg
    17761 gcctacaatc tttgaccagt cgggcttgcc ttcagcccat atgcatgtaa ataccttgag
    17821 tgtctgggct atggcccagg ccaatatgga tgtccacagt attctgttgc taaacaatcc
    17881 tgtatcgggc gcaaacatca gctcaccact accattccct gtcgacaatg tcaattatgt
    17941 ctgtcaactc tgaagcagcg tctcccaaaa ctgcacattt cgtccttgca gaatccatta
    18001 gagccgcaaa ttgccgtcgc gcaccatcga ttccccatac cgagaccgcg gtcaggaatc
    18061 cacttcttcc actgtccctt tgagaatctg catcttcgag gtcgtcacgg atctgaaaag
    18121 caagcccaat gtcggcagcg atcccacaaa ccaccccgag ctcctcggca tttgcgcctc
    18181 ccacgatccc gcccatttcc gccgcacact cgaaaagctt tcccgtcttc agcatctcca
    18241 tccggcacaa ggcaccggaa tcgacacgtt cagatccggg ccacggccaa ggagagatgt
    18301 ccaatacctg cccgccaatc atgccattgc cgcccgccgc cgacgccaat gcctgtacac
    18361 aagagcaggt cgcatcgggg ccaaccacat ccacaaaacc attactcgtg aggacctcaa
    18421 aagccaatgt gagaagggca tctccagcca gaagggccac ggcttcgcca tacgccttgt
    18481 gacatgaagg cttgcctcgc ctgtaatcat catcatccat gcacggcaaa tcgtcgtgga
    18541 tcagcgaata gcaatggacg agttccaggg cgactgaagg ccaaatcact gcatcggtat
    18601 ggcaactcgg atgcactcgt gccacggcag aacaggatgc ccggaccata gcgggcctga
    18661 ttctctttcc gcccggaaat acggagtaac gcattgcctc atgtaatgcc cgcagttcca
    18721 catcgccccc gggcatcacg tcatcgagag ccgcatccac tttttccacg aattcggaat
    18781 accattgggg caacctcaag cttcgtcatc ctccctgtct tcgtctcctg agttgaactc
    18841 ttcgaagggc acggccacgt cccttccatc tttgccctgg actaatacct ccagcttgcc
    18901 ttcagcatct tcgattacgg aggaacaata tcgcaccagc tcagtgcctt cgctgaaaag
    18961 cttgagcgcc tcttcaagcg acggctcgcc tgaatccatc ttttcgacta tcttctccaa
    19021 gcgggcgaga gccgtttcaa aagtcatgtc cgaaggaatg ctcattcttc aacccacctt
    19081 cacatcatgg attgtgcaac cgagttcacc gcagccaagc tgtactgtga cttcgtctcc
    19141 cccggcaacc tggtcagctc tgcgcaatac cgtcccgtct tgtccacgac atatcgcata
    19201 gccccgcccg agaacagcct ctgggtcaag agcgtccagc atcctgccga gatactcaag
    19261 gcgatccttg tttgttcgca acttcgtctg aattgcttga ataagtcgcc tgctcagctc
    19321 gtccacatac tggcgccttt gcattactcg cattcgaact gtgcgaacta gcctggcttc
    19381 caacagctca acatggtggg cataagccga tgcatcaggc acaaccagct ccgcggcggc
    19441 ggacggggtg ggcgcccgca catcagcagc aaaatcggca atagtaaaat cagtttcatg
    19501 ccctacggca gacacaaccg gaactcggga tcgccttatt gccctggcta cttcctcatc
    19561 gttaaaggcc cacaattcct cggcagagcc cccgcccctc cccactatga ttacgtcgat
    19621 gtcagggact tcaccagctc gatcgaaggc ctgaactatg ctggcgggag catccgagcc
    19681 ctgcacaaga gctggaatca ccagtatgtt aattcccggc caacgccttc gagtcacagt
    19741 tattatgtcg cgcaccgcag ctccagtagg ggacgtgatc acaccaaggc gtctcggaaa
    19801 cttcggcagc ggcctcttgg cggcagggtc aaatagccct tccgcctcca gtttggctcg
    19861 gagttgctgg aaggccaggt agagctcgcc gaggcccgcg ggctccatca agtcaacata
    19921 tagctggtat tctcctccac gctcgtaaac gcctacatac ccatgggcgt aaacgcgcat
    19981 tccatcggca gggcggaacc gcaacccaat gttgctccct cggaacatca ccgcgcgaag
    20041 ccgcgaacta tcgtccttca aggtaaaata catgtgtccg gacgaatgat gggtaaaatt
    20101 ggctatttct ccattcaccc agatatcacc caaaaccgga tctcggtcca gggcccgcgc
    20161 cacccgacgc gtaatctcgg atacggtgaa gatctcgtaa gaccctacct ttcccgccat
    20221 ctgcggcatg ccaggcaagt tcggaatccc cgacgcactg aaaatcccgt ctgatccaca
    20281 ggctccacat gttccaatgg acccatgtgg cccaaagcct ccatctgaag ccccagacgt
    20341 acccgccttg taccgacctg ccatctacat tcactccttg ccttcttccc gtgattcgcg
    20401 catgccctcc aaatgcatct gccacgcaag aagcgcgcat cctacggcat tgtcccttga
    20461 atgctcgggc gaagcgtaat acacttttgc gcccgggagc tggtcttcga ttgtctcgga
    20521 gaggatacgc ctgatgatgt agtttgacgc cacgcctccc gcaacaagaa gctcctgcgc
    20581 ccggaagaag cgcaatccct cgcaacacat tgcgccgacc gatctgccta tgcactcgag
    20641 caccgcgtat gccatgtcgg catggccggc ccctgaatcc aattctctca tggcagccga
    20701 gcaagggcca gaaaagctca ccttgagccc actaacggcc accggaagcc tgacccgccc
    20761 cggaacgccg tgtccagcaa ggcgctcaag gtcgggcccc gcagggaacg gaagcccgag
    20821 agccacccct accctgtcga taaactgccc cgcgttgaga tcgccagtgc cccccaaaag
    20881 ctccatttca aacctgtcat ggacactgag aagctcggtc gtgcctccgg aaaggtgaag
    20941 ggcgagaaag cctcctcgag attcgagtcc cgaagaccac ctggccgcgg caatgtggcc
    21001 ttcctggtga gatatctcca agcacggcgc ttcaagggca gccgctacag cgcggccata
    21061 ccctgatgac gcaagaaaga cgggcatgta ggatccttcc atacgtcgcg gccatgcgct
    21121 ggccccgacg gcaacaatgt cgcatgattc ccgcctcgcc acatcacatg cttcctcaac
    21181 aaggcgaggc aggttacgga catgctgaaa cagggcctcc gactgtctga gccctacttt
    21241 gcccttggaa acctgaagcg gcgccctgcc atcataggcg aggatgccgc tgattacaag
    21301 agccactgaa gttgtatagc agctcgtatc aaacccgagt gtcccgacct tcatgcgccc
    21361 cgccaagaca ttcgccccca gaatctcctt ccacgtcgcc gctgctggct gcgtcagatg
    21421 actcggcacg gccctcgccc gcatccgcac catccgctgg gccctggcca tccttgatgc
    21481 gctcagccaa ggcccccaag attccgttga cgaatctcgg agaatcggca gtcccataaa
    21541 gcttggcgat ttcgacggcc tcgtcgatgg cagtagccac aggcacgtcc ttaagataaa
    21601 gcatttcgca tactgctatc ctcatgatat tccgatcggt tggcgacatc cgctggaccg
    21661 cccaatcatg ggcgatcccc gccataacag catctatctc ggtcattctc tccaccgcgg
    21721 cacacacaag ctgagaagca tatccctgcg cccgctcggg aagatcaagg tcggaagagc
    21781 tcgatactgc ataggaaatc gcatcttccg atgtgcaccc tgacatgtcc atttgataaa
    21841 gggctcgcat ggcggcctca cgggcccgcc gcctttctga gcgcatgcgt caaccactcc
    21901 tgtcagtttc gccgacgtcc aaaccgccgc gcaaacctct ccctgaattc gtcattaaca
    21961 tcgatgtatc tgcctacaat aaaaccgatt cccacaaaaa gcgcaaaagc gagcgctcgc
    22021 caaaatcctg cgatcaagac gagcagcccg aagatggcgc ctacgatagc ccccacaaac
    22081 tgtccggagc taccttcggg aacattgaat ctgccgtctc caaaccaagg catgcggacc
    22141 atctcctcca ggcattgccc ccactttatg cgtcgctcag cgccctacgg tcggggcttg
    22201 ctctccatgc gtgatactcc tttaacaacc gtgtgtatgg atttgactgg caaccctata
    22261 acggcatgca ttgcttcgga aagctctctt tccaggtttt cacaaacctc tgggatgcta
    22321 acatcaggat agacatttat gcgcgcatga agtgtgatgc catcctcatt gccctccgct
    22381 gcagcctcaa cgttgcggac accatccacc ttctgggcag ccctctgcgc aatggcctcc
    22441 acagcagcca tggaaatctg cacatcgcca agacctgtcc cagtgataat cggttccgct
    22501 gactcctcta ctctgagagc catcatgatc acatacagag ttacaagaac tatcgcgatg
    22561 ccgattatgg cagctgcaat cctattgctc gccactgaat cataaaaagc aatggcaaag
    22621 gcattgggga atcctccccc cgaaacagac gcaagaacca ccgaaccgag gaagactatg
    22681 attacagcgg tggcaatgag aatcactcgg tcaatggttt tcaatgtggc cacccccgtc
    22741 atcaaggtaa caggcgatat tgctaacgaa cccgaacatc ggtagatgtc tccttctcct
    22801 caatgaacac gttagtcaca tggacattga cttgaaccac ttccaagccg gtcataatct
    22861 cgatcgtcct tttgacgttg cgctgaacct cttcgcagac ctcaggtatt accgcgccat
    22921 atttcaccac gacgttcaga tcgactgccg cctgctgctc gccgacctca accttgaccc
    22981 cacgggccag gttcttcttg ccccacagct cgctccagcc ttccgctatt ccgccgctca
    23041 tccctgcaat gccttcaact tcgatagttg caaggccggc tatgactgct accacttcat
    23101 ttgcgatttt gacagatccc attcccggat cttccataag ctccatattc acacctcctg
    23161 gcaagcgtcc agaggaaccc tcgctgatca tattagcatt ataacagaac tgccggtccc
    23221 caagtagcca atcatagaaa tgaattcacc gcatgcctag attatccttc acggttctct
    23281 ctccattatg ctgatccgcc gcaactccac gcctgtagcg gccgacaccg catcgcctat
    23341 acgcttgact tccgcttctt caagtcttcc cgactttacg accacgcttg cgccagcctc
    23401 cgatagaaag accacagcct gctcgtatcc ttttgcctgt atcatgcttt cagcttcagc
    23461 ttcgcgccgt gctcgttccg ccagggaaag aagagcctcc tgtgcgcgct tgcgcacatc
    23521 tgggtgggta ttcggatctt cgatgagatc ttggatcact tcgcgttcgc gagaacgcgt
    23581 cttgtcgcga tcaatcttga gttcttcgaa aaagtctgct cccgatggca tgatgaaagg
    23641 ctcggcgccg aagggcgtga ccgagcccgg gcccccgacg gacccatact ggtcattacc
    23701 atctgagagg acagatgtcg gcagagtcgc tccacacaaa tcggcagact cctctccttc
    23761 cgacagatcc tcctcactac cgtcgtcttc cgtaacactc aaaccgttgt cgccttcggc
    23821 gtcaactgcc ggcgcagatg agcggcgacg atagaggtcg gggtctacga tcgccgtgat
    23881 tcccaggatc accaccgcca ccgccagcat caggtaggat gccttgagcc agaatctatc
    23941 accacgctgc ccaaagtatt gctttgtctt ctttatcatg gacattgccc acctcccggt
    24001 ctgcgcgtct gcaattcgaa gatttccgcc gacaaggacc gagtgcaagt tttcgatcac
    24061 atccgtgcta gctgcctacg gggacttagc aaccacgacc atgaccctgt gagccggcac
    24121 accaaggagc gtctgtgcag cttcggcaag ctcccgtttg gttcttgaat ccgatgcgcc
    24181 cgacgccgcc accagcactc cggagatagc aggagagcgt tccaaaacca agatgggaga
    24241 ctgtccgcgc cctgaagagg gatcagaagg caagacgacg tcgcgggttt ctcgcttctc
    24301 agtcgtcacc ctcgcctcgc ccgacgacat cttctcatcc acatttttgt attcctccac
    24361 ggaattgacc gcgtactccc ggtatgtgcc gccttccaaa gttatctgga cactgactgt
    24421 gcctgctccc tccacctttg agagaatagc tgccgatctc gtttcaaggt caacccggta
    24481 tctgtctgca ggggacaagt cggaaagccg gtcgcccacg gccgacgaag gaggcgatgt
    24541 ttccgaagaa atcggttcta ggccagaata gccatcaact gcattgccgg aatcacgcgg
    24601 tgaagtctta acaaggctcc ccacgatgat caatagcaca cccaagccgg ccaggagaag
    24661 caatggtttg agccccggca ttttcagcag atccgtacca aatcgtctct cttcgctcat
    24721 cttcttccca cctgccttac aatgatcatc tctgggtcaa cccccaaggc tgcggatgca
    24781 gcggcagcta tttcagagtc gccggggcgg gcacaacctt cagcagcgcc cccaaagctc
    24841 acattcactt ctatccccag cagcgcgcca ctgcgatcga tggatgcccg gacttcccag
    24901 aaacagttct ttccgtagaa ccgggcggca agcgcttcgc gcaccttcgc ctcaataacg
    24961 gtctccgtaa gttccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
    25081 aagtttcctg acatggcaaa cctgtctgcc tccgccagct tgccatcgac gtatcccatt
    25141 ccgatgttga ccaatggagc aatgatgccc gctatcaccg caaggcccat aaccatgtcg
    25201 acgaactttt tcaggcccga cctgggaacg agcatttcaa ggaaagtaac cgccaacacc
    25261 aggacgacga tccccctcac ccacgcggat atggcttcca cgcgccatcc acctccaaag
    25321 catcgacatg gcacctgatt cggcgcccgc cacgttgcct aataacgccc tgattcacca
    25381 agcagcctca agggcagctt gaaccctggc aacaacctaa ctcccttgca gcgcgcctac
    25441 gccaaaaaga gtcagcggat cgccaccacg agcccactca accccacaac gagggatatg
    25501 cagatgaaga acatcaaacc gactgtggtc acacagatgc acaaggcgga gaggacatcc
    25561 gcaagctccc caaggcagtc tgacacgcgt ggatccccca gcggttcggc tactgctgct
    25621 gcaatcttga acatgagcat taccgcgacc atcttgaaaa caggaaacag gcagacaacc
    25681 acgagcgaaa ctaggccaaa ggcgcccaag gcacccctaa caactaggga agacgcagcg
    25741 accaaatcta tcgcgtcccc aaacaccttg ccaataactg gaatcatact ccctgcgagg
    25801 aacttggcgg ccttcgccga cgccgcatct gaaacagaag cgagccctcc acgcacgccc
    25861 acgaacgcca cgaacaaggt tgacgcgaga cccagaatgg tcatagacca ccagcgcaaa
    25921 aggccggcca gtttcgtcac ctgttttcgg tcgctcacat tcccaacaag cgtgagcgca
    25981 gcagcgagca gcaacagagg gatgactgtc gtcttcacca aagtcccaac agtcgtggtg
    26041 gccacgagca ccagaggtga aacgactgcg gcggtcgtca cccctccaga tgttgcaagg
    26101 agtccgacca acgccgggat tatggcatgc ataaaggaga tcatgctgtc cacagcacct
    26161 gtcgcgagat ctgcggacac cgaaaaagat cttattccca ttgtaaccag ggtcatgtag
    26221 catacagccc atgccatggc gcacgggccc tcctccccca atcccgatcc gaaagtgcac
    26281 agtatcgaac cgatcatcgc cactactatc agctccccga ggagcttcag attgactttg
    26341 acttcccgaa ggagaaagga tacggctttc cgcgcgagcc cgccaaggtc gaagattccc
    26401 gctgcttgat ttgacataat ctctattatg ttaaattcgg gcatattctc cgctatatca
    26461 tcatcgaggc cgcggatgaa gcgctcaatc tcggcaacat ccatcgccga tatctgttcc
    26521 attgcagctt ctgccagaag actatcgggg ctatcggcaa tcccggattc gcctcgaccc
    26581 gagcatttgg ccacagccca atgcgaacac aagataaagc agatcatgat gacggcgacc
    26641 ttggcagggc tgcaactgct ccgctcccgc atgcctttcg ggccagtccc aacctggcca
    26701 gcgcctccag aacgcacact tgttttcccc tttttcacca tccggaccgt cccggctatc
    26761 cacgccgatc ctggagttct tcccgcgatt gcctcttctg cgaggccgcc acctcccacc
    26821 tgcactaaag ggagtttcac cagtcctagg cgcatcatga cggaaggatc ctcaggatca
    26881 gatcgagtaa agcaaacatc aatgggacag acatcgccag gatgaggact tttcctgcaa
    26941 gctccacttt tgtagctaaa gccccctccc cggcatcgcg gcacacctgt ccgccaaagg
    27001 ccgcgagata cgccacacca attactctca gaacagtatc gaggtacatt ctgttgaccc
    27061 ctgcacgcac tgcgaggtcg gacaggactt ctacgaagta ggctaggcgc gttacaacca
    27121 tgaggaagac gacgatgccc agagcggccg aaaggacaat tgccatgtcg gaccgagtct
    27181 gcctcaggaa tgcaaggagg accgctccca agatcgccat gcccacaacc t
//
LOCUS       DAOSYL010000028        26906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1476_length_26906_cov_7.427582, whole genome shotgun sequence.
ACCESSION   DAOSYL010000028 DAOSYL010000000
VERSION     DAOSYL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 26906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26906
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_26906_cov_7.427582"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..289)
                     /gene="rpsU"
                     /locus_tag="PLU15_06205"
     CDS             complement(113..289)
                     /gene="rpsU"
                     /locus_tag="PLU15_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOO30550.1"
                     /translation="MSEVKVGKNESLDSALRRFKRQCQRSGVMSEIRKREHYDKPSVR
                     RKKKSEAARRRKYR"
     gene            complement(455..799)
                     /locus_tag="PLU15_06210"
     CDS             complement(455..799)
                     /locus_tag="PLU15_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOO30551.1"
                     /translation="MEECIFCRIARGEIDSSIVYEDENLVAFNDINPQAPMHVLIIPR
                     MHVSSLLELGADGEQLVGKAHRVAGQIARERGYDRSGFRVVVNCGEDAGQSVPHLHFH
                     VLGGRALGWPPG"
     gene            complement(862..2244)
                     /gene="mtaB"
                     /locus_tag="PLU15_06215"
     CDS             complement(862..2244)
                     /gene="mtaB"
                     /locus_tag="PLU15_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOO30552.1"
                     /translation="MRTVAIATLGCKVNQYDSEAIAGEFRRAGFAVVPFEDVADVYVI
                     NTCTVTQMGDKKSRQLIRRANSKNPDALIVAAGCYAQASPEEVAALPGVYVVSGTYDR
                     ARIVEAVLDFEKDSRGLETTGDHDAAAEDTHGSFRPKGANRVPKVLVEDTCVFGGYEE
                     LPADGWTERARAYVKIEDGCENFCSYCKVPYVRGPVRSRELERIRREIERFVVQGYKE
                     VVLTGIDMGAYGRDFGGSPDLADVLAAAANVDGVARVRLSSVDPTDVSEKLIDVMAKS
                     DVICPHLHIPLQSGSDKVLSRMNRRYTMSHYQDVVAMARERISDLAVTTDVIVGFPGE
                     DEEAFQATCDACQRLGFSRMHVFQYSRRRGTVASGLSRQVPRKEKERRSAELIGLGRE
                     LSLQFHRGLVGRVMPVLVEQAEQSQFEGLTPNYVRVYADGPALPGDIVGVRISEADEG
                     FVRGSIERTT"
     gene            complement(2361..2837)
                     /locus_tag="PLU15_06220"
     CDS             complement(2361..2837)
                     /locus_tag="PLU15_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOO30553.1"
                     /translation="MKFDGRSIARAGIIAGVYAGLCLLLAPISYRAFQVRVAEALTVL
                     PIVWPEAVIGLACGALIANVAGPLGVFDVVFGTAATAIAAWLTYRFRNSKMAYFFPII
                     VNGIVVGTYVPFLVGMEIHLWTIPATMLLVAAGEGVAVFALGVPLLNALRRTGMIE"
     gene            complement(2961..3443)
                     /locus_tag="PLU15_06225"
     CDS             complement(2961..3443)
                     /locus_tag="PLU15_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30554.1"
                     /translation="MKRLLDGFLLLSLLTVVVTTMTGCTLGRARIGITVNPDPLLIDF
                     NKSEIEGTVTLTLDGLGSFTVDEIGLDYIDAQGQRIEGEGLPQGGKLTQSISLVGTMG
                     VVRKTLPLKGVLGAEGVKIRNEWWLQGALHPASCRMTLLDISGRVVGAGSVGLKWHPY
                     "
     gene            complement(3594..4055)
                     /locus_tag="PLU15_06230"
     CDS             complement(3594..4055)
                     /locus_tag="PLU15_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30555.1"
                     /translation="MKRAHVLRGIAIAAVLVMASMTAGCLKTEIAMSISPGELVCVRD
                     AKFDGTITVEMKGFLAGGKYDTLTVKFFEGGNDEPLATMRVDDLMIVVSPLGKIVKQE
                     LAELDGLVAPAVLWDDEGVFRGAKAQFTLTSTGLGVQLNPLVCEVGLVEAR"
     gene            complement(4743..5090)
                     /gene="trxA"
                     /locus_tag="PLU15_06235"
     CDS             complement(4743..5090)
                     /gene="trxA"
                     /locus_tag="PLU15_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOO30556.1"
                     /translation="MANSVTPIEITDASFGAEIGDFSGVAIVDFWAAWCGPCRMQGPI
                     IDKLAEEYHAKDEIVKVAKLNVDENPRTAEKYGIMSIPTVIIFKNGKPVDKVVGVTPL
                     AVLKRRVEQVASV"
     gene            complement(5280..6032)
                     /locus_tag="PLU15_06240"
     CDS             complement(5280..6032)
                     /locus_tag="PLU15_06240"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOO30557.1"
                     /translation="MRRFFVGCDAISGDLVTISGRDARHIVKALRLKLGDAIVVVDGV
                     GRRYVARITATRETTVEARIESMCDSGVSEPHVPITLVQGLPKADKMDGIIQKCTEIG
                     IARIIPVMSERSVLRPDGASAERRMARWRRIAEEAAKQSGREAIPLVADIGTLKSAIE
                     MLRHEGALMVFPWELEQEYSLHQALSELNPSSFPSVGFFIGPEGGFSHAEVEYARAHG
                     ATTVTLGQRILRTETAGPVVAAIILYHMGELG"
     gene            complement(6034..6972)
                     /gene="prmA"
                     /locus_tag="PLU15_06245"
     CDS             complement(6034..6972)
                     /gene="prmA"
                     /locus_tag="PLU15_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOO30558.1"
                     /translation="MIWAEVSCEVSDEDSEMVAEVFRMAGAGGVAFTGPSLITDSMAV
                     AESSEAALYPEGACFGGPTRVIAYFPVDNLLEWRIGTIKRGLEEMWALKGGCLPCPTI
                     MLRQVENSNWAAAWKSHYKVEHVGDRIVVVPSWVDYSPREGEVVILLDPGEAFGTGQH
                     ESTRGALAAIESHVVPGARVLDIGCGSGILSVAAAKLGAARVYGVDSDSVAVAVARSN
                     VDENKVSHVVSIGLGDLASGISERYDVVVANILAGVVARLASDVPRLLYPKGKFISSG
                     FVAKQEESVRRALESVGHSVIDRLQFEDWVTLVSEG"
     gene            complement(6987..8141)
                     /gene="dnaJ"
                     /locus_tag="PLU15_06250"
     CDS             complement(6987..8141)
                     /gene="dnaJ"
                     /locus_tag="PLU15_06250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO30559.1"
                     /translation="MFATGNRDYYEVLGVERNASDEEIKKAYWDLARKWHPDVNPNDS
                     SAEAKFKEINEAYQVLSDPQKRARYDRYGHEDGVAGGGWSQDFGGFDPFGSIFDAFFG
                     DSPFTRAAYPESRGPVRGHDLKATVEITLEEAATGVNKEISVDRMTMCERCGGEGMEP
                     GTSRTTCPECAGRGQVHSMSRTAFGTFTRVTTCPKCRGEGTIVDFPCNDCGGAGCVKK
                     TERIEVNVPAGVDSGSRVRIRGAGDAGLRGGLAGDLYVYIKVKPHSVFKRDGNDLRIE
                     VPVSFPQLALGGEIVVPTIDGSKMTINIPRGTQTGEEFRQRGKGMPILRGFARGDMIV
                     TVRVVTPQKLSEREEELLRELAQIQGASAGETGGKKQSRWGKVLHRRDKS"
     gene            complement(8155..9207)
                     /gene="hrcA"
                     /locus_tag="PLU15_06255"
     CDS             complement(8155..9207)
                     /gene="hrcA"
                     /locus_tag="PLU15_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOO30560.1"
                     /translation="MAENMADRKKAILRAVTDDYISTAEPVGSRTIARKYGLGVSSAT
                     IRNEMADLEEEGYLEQPHVSAGRIPSDKGYRFYVDSLMPERNLTGLEEKKIRAECAKR
                     SDEIQSLIRATAKVLGELSNCASIVIGPGVKTAEIRHIQLVPFSSDSILVLVVTNAGL
                     VEHKILSIEHEMNPVDLENMSRMLNHRLRGRSLSQMRGKVLRDILSDLDAYASFLEQM
                     FELLLSAIRSSDENRVYVDGLMKLLGQPEFSEIERARPILEFLESDDLVLTELSGSAS
                     RGLGGAQVTIGSENTREELRECSVICAPYGIRGQVVGTIAVVGPTRIDYSLTIALVAH
                     IAESLSDLLTRTQSIK"
     gene            complement(9330..10553)
                     /gene="hemW"
                     /locus_tag="PLU15_06260"
     CDS             complement(9330..10553)
                     /gene="hemW"
                     /locus_tag="PLU15_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOO30561.1"
                     /translation="MNQNSGGVYVHVPFCVRKCSYCDFISYPAGDNIQVMEAYLSSVV
                     DELRLRKTELEQIRPITTLYVGGGTPTCVPWQKLAPFVSTLIAEVEKINASDPTYGGF
                     DSADLETTVEANPGTVDPRGLAALRQSGVNRLSLGFQSLDARELASLGRVHSPEDCLR
                     AYEWAREAGFDNVSVDLILGAPGQTGQSLAQTLDAVISLGPQHLSAYCLSVEEGTVLA
                     RAIASGVQTVPDDDLVAEFYEWLVSRVEDAGYVRYEVSNFAIPGFESVHNIRYWTCGY
                     YVGLGVAAHSHLPAGAGGSVRSWNVESVDKYVASISEGTRALSGFEERTAFEEAQDRL
                     MLGLRMSEGIDLASIGRLYGEDLLGEYGARFADLAEKGLVNFSGGKVRLTPRGFLLGN
                     LVFSECVDCGDSAQS"
     gene            complement(10550..12355)
                     /gene="lepA"
                     /locus_tag="PLU15_06265"
     CDS             complement(10550..12355)
                     /gene="lepA"
                     /locus_tag="PLU15_06265"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOO30562.1"
                     /translation="MNIEGIRNFCIIAHIDHGKSTLADRILELTGAIDERSMVEQFLD
                     KMDLERERGITIKLKPVRIRYARPNGEVYVLNLIDTPGHVDFTYEVSRSLAACEGAVL
                     VVDATQGIEAQTLANLYLAVDNGLEIVPVVNKIDLPNAEPERVREELVDLLGVGPEEV
                     IFTSAKTGEGVLDVLEAVVERIPPPSGDRDKPLRALVFDSHFDPYRGVIAHIRIVEGM
                     VKVGDVVKVMSTGTEHDVTELGVFRPEMEEVECLGPGEVGFLVAQIKEVGDVPVGDTI
                     TSAENPAASPLPGYRPAVPMVYCGLYPLENSAYGDLRDALEKLKLNDASIAYEPETSA
                     ALGFGFRCGFLGLLHMEIVQERLEREYNLELITTAPSVAYRVNKMSGECIMVDNPANL
                     PPVAQIETIEEPFVRTTIFVPSDYIGPVMELAVERRGEVHDMHFIGPTRVRLTYDMPL
                     AEIIYDFFDKLKSRTRGYASMEYEFLEYRPSDLVKLDILVGGKPVDALSSIVHADFAD
                     KRGRSLVERLRKLIPRQLFDVPIQAAVGSRVIARETVKAVRKDVLAKCYGGDVSRKRK
                     LLEKQKEGKRRMKQFGHVEIPQEAFMAVLADDDEL"
     gene            complement(12480..12734)
                     /locus_tag="PLU15_06270"
     CDS             complement(12480..12734)
                     /locus_tag="PLU15_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30563.1"
                     /translation="MRRIDQWETGRGRRAFAVVPAIACLFLIVVFIAIAVAGSNSSQL
                     AGAGSQWSPVRIRAVGGGYHGIVLEAFGRAVRIEFENALK"
     gene            complement(12738..14237)
                     /locus_tag="PLU15_06275"
     CDS             complement(12738..14237)
                     /locus_tag="PLU15_06275"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOO30564.1"
                     /translation="MQRGRGTRRKSRRAGGLKIGPLGGLFVFYVILCFAAVGLIIGTR
                     AVKDGVSVPAVSGVPGQDVVGDGETLASEPSLWSKVSKMVSSFLSLFRPSSDRSLPNR
                     SAEERFGSIGPRLVNAALRAMGGLDFADPRTLIRAELPSILAAQRTSDNPSRSATYGR
                     GSGEDPDISAPMRAEGSLVSNSTLVSGQLSDAELESLTSSGLLVFGDQPSSSDPEAGT
                     EVPDGTSVQVIVSSESANAAVPAVSKPVVESSSNTSSPGRDADSQGANSVPTREPVPS
                     QPSKFPQLAAREIADNMVLRGASPKIAILHTHTSEAYRATSGRDYCWGKPDGVVKVGA
                     AMAEELSEVLGVPSIHASTVHDWPDWTLSYSKSLKTMTSLVKSYSSLRAVVDIHRDSL
                     PANREKLKTAMVGGEKVARVLFVVTDDSSGLAHPNWRKNYHFALELSAEMDKMYPGVS
                     RGVAIVKNARFNQHVHPGAIILEIGGTDNTVEEACRSARLVARALARII"
     gene            complement(14295..15380)
                     /gene="gpr"
                     /locus_tag="PLU15_06280"
     CDS             complement(14295..15380)
                     /gene="gpr"
                     /locus_tag="PLU15_06280"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOO30565.1"
                     /translation="MGFDRNFGAKWRPSKELQHLSECDMGLSGGGYLTDLAVEANELV
                     QRASRAQVPGVSSQVEEMDFGSITRVRVQTEQAARSLGKAVGNYVTIDAPELPLRDKG
                     LQEDVSEALARELVSLIRANFNNVDFWTVPDFTTLVCGLGNWNATPDAVGPMVAGEVL
                     VTRHVYSLTPPEKRGGLKSVAALSPGVLGLTGVETAEIIAGVVARVRPHLIIVVDALA
                     ARSVSRLGTTIQISDAGIQPGSGVGNRRFGINRESMGIPVIAVGVPTVVHALTIISDA
                     LEIVGSSQQPGVGRPAAGPLSELGVGQPGLPPAVAQMLQPYFGTLIVTPKEVDVLVKE
                     LSHVVAGGINYALHPAIDEDEIYLYLQ"
     gene            15975..16259
                     /gene="rpsT"
                     /locus_tag="PLU15_06285"
     CDS             15975..16259
                     /gene="rpsT"
                     /locus_tag="PLU15_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOO30566.1"
                     /translation="MPNTRSAKKRVRQARTRTLRNTSVKSSVKTAVRKCHEAMDELGP
                     EACAKELNHAYSTIDRAAKKGVIHPNKAARLKSSLATKAVNMGKAPEAGR"
     gene            complement(16355..17389)
                     /gene="holA"
                     /locus_tag="PLU15_06290"
     CDS             complement(16355..17389)
                     /gene="holA"
                     /locus_tag="PLU15_06290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOO30567.1"
                     /translation="MRADLEQAIRAGRIAPIYILYGEDSYSRQVNADELAQAIAAARG
                     GADLNVYDMSESSVDDIVTAILTVPMFASSRVVVVRRFEEISAAQQGRIISLIAPGKD
                     RASLQMPEGTCVIFTSNSTSLPRKAVAAAGGNLVAVEFRPAYLKDARSYAYSRAKELG
                     VEIEPEAVELLLEFVGADYGAITQEMDKLITYLGPDKSVGRMRITVDDIRTAVGRSPT
                     QTVFEFCDAVIGGDCSGAMRALDDLMSTERHPLSILNTLATQVRRVMSARCELDRGLT
                     GDEAARAISRSPGKADWAARKAVGQAAKLSQADIARMISRIARADLDFKTGTMPDRLL
                     MEVLIAEICA"
     gene            complement(17460..18293)
                     /locus_tag="PLU15_06295"
     CDS             complement(17460..18293)
                     /locus_tag="PLU15_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30568.1"
                     /translation="MSKRRKTVGMSIVYILLTVGAVIMLLPFYYMLITSVKGPEELYT
                     PELVWFPKRFVWSNYSEAWNADPNFPRYFFNSAFIATITTALQLVTSALAAYAFAMID
                     FAGKETVFMILLGTMMIPSQVTLVPNYAIIAKAGWIDTYKALIVPFAASAFGIFMMRQ
                     FFQTIPMELWDAAQIDGCSRFEYLWRVIVPLSKPVFVTNGLFTFIGGWNSFLWPLIVT
                     NSSTYRTIQVGLSQFNQEFGTVPNLLMAASTIAIVPLVIIFFIAQNQLIEGIARTGLK
                     G"
     gene            complement(18310..19212)
                     /locus_tag="PLU15_06300"
     CDS             complement(18310..19212)
                     /locus_tag="PLU15_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30569.1"
                     /translation="MNSLERKRYVKDTVAAYMYLAPALIVIIVFNLFPAFYALYISLF
                     DTNLIGRWDWVGLGNYSELFRDADFLQSLVNTGKYVLGTVPVGMALALLIAWLLNKPI
                     RGLSAYRTAYFLPYVTPIVAISIVWTWIYKEDSAGMLNSLLCLFGAEPQAWLLDPKWS
                     MFALCLMSVWRHLGYNVVLFLAGLQNIPAEYYEAAEIDGASGWSMFAHITWPLLSPTT
                     YFILMVSIIGSFQVFTQVYVLWPSVSGGPLGTTRVVVKYLYDQGWGAFRFGYASAIGY
                     ILFAIIFVLTLVQKDLVGKRVHYQ"
     gene            complement(19209..21335)
                     /locus_tag="PLU15_06305"
     CDS             complement(19209..21335)
                     /locus_tag="PLU15_06305"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOO30570.1"
                     /translation="MHKQPISRRRLVLVWATLFSVMAGCVCLGAEPVVELNCYFGNLH
                     SHTSYSDGVLTPSDAFRHARDVAMMDFLAVTDHGYYLQEATNIHHWFKSLEEADEVYQ
                     PGVFVSMIGFEWTFTDGHMNGLDTPLAASRDTQRNLAAFIDFLAHYNGIGVFNHPNYD
                     IQPNWNDFEYLGEGDRFVCLLEVGSGPYRHNIRNERAYCRALDRGWHVGAISCQDNHK
                     ADWGTAAPTRTAVLASELTREAIYESLRSMRTYATEDSNVRVSFTSGDCQMGSDIIIS
                     RDDIDSGRPVYFEIVIDDPDEDDMLSSVEIITNGGVVVWEREVCGTGVFRADVEIMPA
                     QSYNWYYVRAVQADMDVIVTSPIWVSTGSQIAVSDLGCVDLAPREGNPARVCAEIVNR
                     NDVPIHGATVLLYEMREGAPTFISSAQVDLPAGRGMPVSFEWTPSKAGDVSFVLELVA
                     CGEGARDIYCGKSARVRSSDIKSVVIDEGHNNRCSGYMDAFARLLGEADYNPRINEGR
                     IDAQTLDGADILVINVPETGFSLTPTSFDDEELDAISSFVAAGGSLLLAGGSDFPGLR
                     SCDQFDLLLEQMGSAIRFNRDEMRRDDLSVLDVEWGLERAVYADEACSLAGPGWIDLS
                     EVPQVVVLAHAPGAWSADVSGEGRLFQYAGSPVFAAAETVGSGRLVCLGAPVYSSRDL
                     ARERCGNGAFVLSIISWLAGGEWNRQ"
     gene            complement(21496..22761)
                     /locus_tag="PLU15_06310"
     CDS             complement(21496..22761)
                     /locus_tag="PLU15_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30571.1"
                     /translation="MKRFATIALMAAIILATTAVGPSMAAGKTTITFWHAMGAQLGKT
                     LEGLVEEFNRQSPDVYVKAEYQGNYGALSQKIVGALVARKPPTMAQVYGNWAAEYISG
                     NELVPIQKFVKGPNGLSQLEVDDIWDGLREGCMFDGVWYTMPFNKSVYVLVYNQTALK
                     EAGISGPPKNWQELVNTAKALTVKDGDKIVRYGFGLRPNIDIFACFFFNAGGSWLNPQ
                     GKVAFNSPAGVTALQFMADMLNKYKVSYYVPGYLDADFGAGKVAMYFTTSPGLSYTEN
                     SVAGKFEWSAAPLPYMDPKFRSTPVAGTDLAIFSRATPEEQEAAWKFIKWLVEPRQTA
                     KWAIGTSYIPVRKSAMHLDMMKKWFAVHPRDEQSLQQLRYVKYDPNMAAWNNIRGDVS
                     EAVEKVFLGKATPKEALDAAAAKANSRIR"
     gene            complement(22871..25477)
                     /locus_tag="PLU15_06315"
     CDS             complement(22871..25477)
                     /locus_tag="PLU15_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOO30572.1"
                     /translation="MALTAGNENPGKHLFGFGTSVTHGNLGACAVAPVIALFLIVGIL
                     LGFREAGFCLRWAWLVLAGIAFARIETLRLSKKRRAVLAWAVLALAGISLGAVAMERT
                     DGMAARSIARYAGRDVMVRGTIRSVWPYVDPAELILDVDTVMDQGSNGSAVGAVRIRP
                     RWGHEEDWVTWDVRVGDIVWARVQVEYADRPMNPGEPDYRMILLQQGIHAVAWAEHDS
                     MVFAGRNGRRPLLLVADAVRRQMVASASAVLDPPERDLLAGMMFGDYRGVDPIILESF
                     RKSGAAHLLAASGLHVGLVAGLFGALVGIVGASPEAADIAAVLFGGIYAVAAGLRASV
                     VRAWLMLTVAVLAKARRRKGSLIHPLAIGASVQLLINPLLLWNAGFQMSYLAVGALAA
                     FAPFATRPRENAPLGLSALIIQVLTVSVGIQIATWPIIANMTGQVCFVGPLTNLAALP
                     LASVVMTAGVVGCAIYPFLPWVGSAIITGVGVVLRVLTHLVGWMSSLPGATICCRKLR
                     SFELVAYYLMVSVIWWLAWDRFARFRAARRLSRICRDKLGIVLTIAALILGVVIAWPR
                     PLEVVFLSVGQGDAAVLFTPEGHTLLIDAGTERAGERHVLPYLERCGKRHIDAAIITH
                     EHLDHAGGFAALAGKVQTDTVMIPRGMDQAWIDGILVGSECQYSNAPSLAQLVRGNQI
                     NAGSVRLEMINPIVYGGGPEAVGDGLRGTSLEGDVGFSGNLTFDPNEASLVFHVVCGE
                     FSMLLCADAGRKFEQEVFRCLGPEGHGVLRADVLKVGHHGSSSATSARFLEAVKPSIA
                     VISVGRNSYGHPSQDTVERLLQSGAAVLRTDEVGAVRVEWHPKSHRLTVSDMRALWQT
                     APMFRKLETLDK"
     gene            complement(25478..26164)
                     /locus_tag="PLU15_06320"
     CDS             complement(25478..26164)
                     /locus_tag="PLU15_06320"
                     /inference="COORDINATES: protein motif:HMM:NF018247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO30573.1"
                     /translation="MNKLSPRKIAAIVAAVGLLGLAAVALKQARRPALVVIENEETDS
                     SYQDGSIHGGDCLSCETDVEGPVAGSVTPELDDDLIVIHVAGAVARPEVYSLPHGSRV
                     ADAVRAAGGATSEADLDMVNLALPVHDGEQICIPRKEPTPSGSSEARIPSNRADISAL
                     SPKETLININTASVAELEQLPGVGPVTAAKIVEYRKINGPYSSVNDLLGVSGIGEAKL
                     SKIAPHATVR"
     gene            complement(26714..>26906)
                     /locus_tag="PLU15_06325"
     CDS             complement(26714..>26906)
                     /locus_tag="PLU15_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30574.1"
                     /translation="NQREDNPQMCYKRSEGLRSFSFGFCSQDLLDYGASCCRFGCRLG
                     NEGMDQSLGLLICQPLQKC"
BASE COUNT         6393 a         8412 c         6225 g         5876 t
ORIGIN      
        1 agcctctccc tgattgtaga cccgagcacc ccatacgtgt agggccagcc cgaacgacga
       61 aacagcaata tttacttttt tacgcactat gggggtatgg ccgaaaccgc ccctagcgat
      121 acttgcgtct gcgggcagcc tcagactttt tcttcctccg aacgcttggc ttgtcataat
      181 gctcgcgctt gcgaatttca gacattactc cggatcgctg acactgtcgc ttgaaacgac
      241 gcagtgcgct gtcgagggac tcattctttc caactttaac ctctgacata cagatacccc
      301 ccctccgtgc gagagtaacc aaccaccatt gctcgcttgc ccataagcga atcctttgac
      361 gcgcagggca atcttgtcaa attatacttt actcactgcg taggtgtcaa acgaaacgac
      421 aggtttaatg ctcaccgcgc ctcggtacac atcatcaacc tggcggccac cccaaagctc
      481 tgcctccgag cacatgaaaa tggagatgcg gcacactttg ccccgcatcc tctccgcaat
      541 tgactactac cctgaacccg ctccgatcgt atccgcgctc gcgagcgatc tgccccgcga
      601 ccctatgcgc cttgccgact agctgctcgc cgtcggcgcc gagctccaaa aggctagata
      661 catgcatgcg agggatgatc aacacgtgca tgggcgcctg agggttgatg tcgttgaaag
      721 ccacaagatt ctcgtcctca tatactattg acgaatcgat ttccccgcgc gcaattctac
      781 aaaatatgca ttcttccatt cactctacac cccctcggct gatttcacaa aggtacctgg
      841 gcagccgcat atgcggtcga atcatgtagt cctttctatg ctgcccctca caaacccttc
      901 atctgcctcg gatatgcgta cacccacaat gtcgccgggg agcgccggcc cgtcggcgta
      961 gacccgcacg taattcggag taaggccctc aaattgactc tgctcggcct gctcaaccag
     1021 gacaggcatg acacgaccga ccagtcctcg gtggaattgg agagacaact cgcgccccag
     1081 ccctatcagc tcagcgctcc tcctctcctt ctccttcctc gggacctgcc tggacaggcc
     1141 ggaagcgaca gtgcctctgc gacgcgaata ctgaaatacg tgcattctcg aaaaccccag
     1201 tcgctggcag gcatcgcaag tagcctgaaa agcctcttcg tcctcgccag ggaagccaac
     1261 tatgacgtca gtcgtaaccg ctaagtcgga tatgcgctct ctcgccatcg caactacatc
     1321 ctggtaatgc gacatagtgt agcggcgatt catccgtgaa agcaccttgt cgctgcctga
     1381 ctggagcgga atatgcagat ggggacagat gacatcgctt ttagccatca catcgatcag
     1441 tttttcgctc acatcagttg gatccaccga gctcaagcgg actctggcta cgccgtccac
     1501 gttcgcagcc gccgcaagaa catccgcaag gtcgggggat cccccaaaat cccgaccata
     1561 tgcacccatg tctatccccg taagcaccac ttccttgtat ccttgaacaa cgaagcgctc
     1621 gatctccctg cgaatcctct ccagttccct gctacgcaca ggccctctca cgtaaggaac
     1681 cttgcagtag gagcaaaaat tctcgcatcc atcctcaatc ttcacatacg cacgagcacg
     1741 ttcggtccac ccgtcagccg gaagctcttc atagcctccg aaaacacatg tatcttccac
     1801 gagcaccttt gggactcgat tcgccccttt gggtctaaat gaaccgtggg tgtcttccgc
     1861 cgctgcatca tgatcccctg tcgtctccag ccctctggaa tccttttcga aatcaagcac
     1921 ggcttcgacg attctcgctc ggtcatatgt tccagagacc acgtataccc caggaagcgc
     1981 cgcaacctct tcgggcgaag cttgcgcata gcagcccgca gcaactatca gagcatccgg
     2041 attcttcgag ttggccctcc gtatgagctg cctcgatttc ttatcgccca tctgagtaac
     2101 agtgcacgtg tttatcacat acacgtcggc gacatcttcg aagggcacaa ccgcaaatcc
     2161 ggctctcctg aattcccctg cgatcgcttc ggaatcgtat tgattcacct tgcacccaag
     2221 tgttgctatg gcgactgtcc gcacccgaaa gatacctccc aaatgtgcat ggcatgtccg
     2281 gtctcctgcc aaagactggc ccacttgacg cctccgttgc ctacaagagt cgtgcgctaa
     2341 gcgagtccta ccttgttgtt ctattctatc atgccggttc tccgaagagc attcagaagc
     2401 ggcacaccaa gagcaaaaac ggcaactccc tcgcctgcag ccacgagaag catggttgca
     2461 ggtatcgtcc agaggtgtat ctccatgccg acgaggaacg gaacgtacgt cccaacgaca
     2521 atcccgttga cgataatcgg aaagaagtag gccattttgg aattcctgaa gcgataggtg
     2581 agccatgcag ctatagccgt ggcggcagta ccgaacacca catcaaacac gcccagggga
     2641 cctgcgacat ttgcaatgag tgccccgcac gcgaggccta tgactgcttc cggccagaca
     2701 ataggaagca ctgttaaggc ctcagccact cttacctgaa aagctctgta tgaaataggg
     2761 gccagaagca gacacaaccc tgcgtacacg cctgctatta tcccggcgcg cgctatggat
     2821 ctaccatcaa acttcatctg ccctactcct ttcgccaaca tcgcacgtac ctaattctgc
     2881 gcacatccct agcgcaatac tcgtatgctg ctttcagtct ccggtagagc tccacatcca
     2941 tgcctgatat gaggcatggc ctaataggga tgccatttca accccacaga tcctgctcca
     3001 accaccctgc ccgatatgtc aagaagagtc attctgcagc ttgcagggtg caaagctcct
     3061 tgaagccacc attcattcct gatcttcacg ccctctgcgc cgagcacgcc ctttaagggc
     3121 aatgtcttcc tgaccactcc catggtcccg acgagggata ttgactgagt gagctttccg
     3181 ccttgtggaa ggccctcgcc ctcaattctc tggccctgag catcaatata gtcgagccct
     3241 atttcgtcaa cagtaaagct ccctaggccg tcaagcgtca atgtgacagt gccctcgatc
     3301 tcacttttat tgaaatcaat caaaagtgga tcgggattca ctgttatccc aatcctagcc
     3361 cgtccgagag tgcacccagt catcgttgtg accacaaccg taagcaacga aagcaacaaa
     3421 aacccgtcaa gtagccgttt catgaatccc gcctcccatg gccgcgtact cgcccaacgg
     3481 caaaccgcca ggcgcagggc ggatatgata tgaaataggg ggcctgtcgc ggcagcccgc
     3541 ggacaggagc ccctactacc agtgatcatc cgatcacgtc acattgctct atcctatctc
     3601 gcctcaacca atccaacttc gcatacaagc ggattgagct gaacgcccaa ccctgtgctg
     3661 gtcaaagtga attgcgcttt ggcacctctg aacacgccct cgtcatccca taacacagcg
     3721 ggcgctacta gaccatccag ttctgccagt tcctgcttga ctatcttgcc gagaggcgat
     3781 acaacgatca taagatcatc aactctcatc gttgccagcg gctcatcgtt gccgccttcg
     3841 aaaaacttca ctgtcagggt gtcatatttt ccgcctgcta gaaacccttt catctccaca
     3901 gtgattgtac cgtcaaactt ggcgtccctc acgcaaacaa gctctccggg agaaatgctc
     3961 atagcaatct ctgtcttgag acatcctgcc gtcatagagg ccatgacaag aactgctgcg
     4021 atggcaatac ccctcaaaac atgagccctt ttcatcaata gaccctccca catgacgcaa
     4081 ttggaaccac agcattgcgg taggataatt ctactagaag acctatattc ctgctacttg
     4141 tcccagatgg gcctcactca tcgtgcaagc aattacggcg caattttcaa gacatcccac
     4201 gatattccct caagctcatt ctgagcatgt aggccttgcc atgtcgtcgt ttcttccagg
     4261 aacctgctcg gacccccaaa aaaagcaatt gggaagcgta tcgagccgag tttgttgcga
     4321 gcggtgtttc accatgtaca cacctgtgag gtgatttgcc aggtttacca aggggaggca
     4381 gtgattctac gggaattgcc gcctcgcaat atcagttccg tccggtctac cttcagagcc
     4441 gttccttaca gcaccagcca tgtcccaacc gttcaactac gaacgactga tggtatcgtc
     4501 cagaataaga acctccctcg acattggctg ttaatgcaac cgtaggcgtt tggagcggag
     4561 atttgcgaaa ctggactaag cacggcgcct taccaaaaaa gaatgcccca tttcgatgcc
     4621 gcgttcccgc agctggcatc ataacagggc actgagaagt cttaataccc tgaccaaatc
     4681 gccagagtaa acaggataga gcaagagcca gccttcctgc gcttcagcaa atccgactcg
     4741 actcagaccg aggcgacttg ctccactcga cgtttcaaaa cagccaacgg cgtgactccc
     4801 actactttat ccactggctt cccatttttg aaaatgatga cagtcggtat gctcataatg
     4861 ccgtatttct cggctgtccg aggattctca tccacgttga gcttggcaac tttgacgatt
     4921 tcgtccttgg cgtggtattc ttccgccagc ttatcgataa tagggccctg cattctgcaa
     4981 ggtccgcacc aggcagccca aaaatctact atagcgaccc ccgagaaatc gcctatttcc
     5041 gcgccgaaac ttgcgtccgt aatctcaatt ggcgtgacac tatttgccat cacacagtca
     5101 gtcctccctc aagatccacc ccacattgcc cgcgcgatcc cgatctctgc atcattagtg
     5161 gcagccgtat acggtatggg ggtatcctca cttgaattat agatccatgg ttgaaaggtg
     5221 tcaacagatt ctgcaaacct aaagcagcac ggcatttttc gcatcttcca aggcttattc
     5281 tacccaagtt ctcccatgtg atagaggata attgcagcga ctaccggacc tgctgtctcc
     5341 gtgcgcagga tcctctgccc caaggtaaca gttgtcgcgc catgggcgcg ggcgtattca
     5401 acttcggcat gggagaaacc tccttctggg ccaatgaaaa agcccacact agggaatgac
     5461 gaaggattga gctcggagag ggcctgatgc agactgtact cctgttcgag ctcccatgga
     5521 aacaccatga gagcaccttc atggcgcaac atctctatcg ctgacttgag agtgcctatg
     5581 tcagcaacta gtggaatcgc ttcgcgcccg ctctgcttcg ccgcctcctc cgcaattcgc
     5641 ctccatcgcg ccatccttcg ctcggcagaa gctccgtcag gcctcagcac actccgttca
     5701 gacatgactg gaatgatcct tgcaatccct atctccgtgc acttttgtat gattccgtcc
     5761 atcttgtcag ccttcggtaa tccctggacc aacgtgatgg gcacgtgagg ttccgaaaca
     5821 cccgaatcac acatgctctc tattctggct tcgacagtcg tctcgcgggt tgccgtaatc
     5881 cgcgccacat atcggcggcc cactccatcg actacgacaa tggcatcgcc aagcttcaga
     5941 cgaagtgcct tcacgatatg gcgcgcgtct cttcctgata ttgtcaccag atcgcctgat
     6001 atagcatcgc atccaacaaa gaacctgcgc atttcaaccc tccgaaacca acgtcaccca
     6061 atcttcgaac tgcaggcgat cgatgacgct gtggccaaca gactccagag cccgccgcac
     6121 cgattcctcc tgttttgcca cgaaccccga tgatatgaac ttgcccttcg gatacaggag
     6181 gcgtggaacg tcggacgcaa ggcgcgcgac cacgcctgca agaatgttgg cgactaccac
     6241 atcatacctt tcagagatgc ctgacgcaag gtcaccaaga cctattgaaa ctacatgaga
     6301 aaccttattc tcgtccacat tcgaacgagc tacagccacc gcaactgaat cgctatctac
     6361 gccgtaaacc cgagcggctc ccaatttcgc tgcggctacg gacagaatcc ccgatccaca
     6421 tccgatatcc agcacgcgcg caccgggcac aacatgactt tctatcgcgg cgagcgcgcc
     6481 tctggtcgat tcgtgttgcc ctgtcccaaa agcttcaccc ggatcgagca atattaccac
     6541 ctccccttca cggggagaat aatcgaccca tgacggcaca acaactattc tatcgcccac
     6601 atgctctact ttatagtgag acttccacgc cgctgcccag ttggagttct ccacctgcct
     6661 cagcatgata gttgggcacg gtagacaccc gccctttagg gcccacatct cttcaaggcc
     6721 cctctttatg gtaccgattc gccactcgag gagattatcg acggggaaat aagctataac
     6781 ccgcgtgggc ccgccaaagc acgcaccttc ggggtacaac gccgcctcgc tactctccgc
     6841 aacggccatg ctatcagtaa tcaatgaggg gccggtgaaa gccactccac ccgcccccgc
     6901 cattcgaaat acctcagcca ccatttcgga atcttcgtcc gatacctcac acgagacttc
     6961 agcccaaatc acatcgatac accgccttac gacttgtccc gtctgtgaag taccttcccc
     7021 cacctagact gctttttgcc cccggtctca ccagcgctgg cgccttggat ctgtgcaagt
     7081 tcccgaagaa gctcctcttc tctttcgctc agtttctgtg gagtaacaac tcgcaccgtc
     7141 acgatcatat ctcccctagc aaacccgcgc aaaattggca tccctttgcc acgttggcga
     7201 aactcttcac ctgtctgagt tcctcggggg atgttaatcg tcatcttaga cccgtcgata
     7261 gtcggaacaa cgatctcgcc gcctagggcc agttggggga atgaaacagg cacttctatt
     7321 cttaaatcgt ttccgtctcg cttaaacacg ctatggggct ttactttaat atagacgtac
     7381 agatcaccgg cgaggcctcc ccggagtccc gcatcgcctg cacccctgat ccgcaccctc
     7441 gacccactat ccactcctgc gggaacattc acctctatgc gctctgtttt cttgacgcaa
     7501 ccagcgcctc cgcaatcatt gcacggaaag tccacgattg tcccctcgcc acggcactta
     7561 ggacacgttg ttaccctggt gaaggtgcca aaagccgttc tgctcatcga atggacctgt
     7621 ccgcgtcctg cgcattcagg acatgtcgtc cgagaagttc ccggctccat cccctcgccg
     7681 ccacatcgtt cacacatggt catcctgtcg acactgatct ccttgttgac ccctgtggcc
     7741 gcttcctcca gcgttatttc gacagttgcc ttgaggtcat gccctctcac aggtccccgc
     7801 gattctggat acgcagcacg cgtaaacggg gaatcgccga aaaatgcatc aaaaatactg
     7861 ccgaacgggt caaatcctcc aaaatcctgg ctccatccac cgccggcaac tccgtcctca
     7921 tgtccgtatc tatcatacct agctcgcttt tggggatcag agagcacctg ataggcttcg
     7981 ttaatctcct tgaacttcgc ttcagccgag gagtcattgg ggttcacatc cgggtgccac
     8041 ttgcgggcaa gatcccagta tgccttcttt atctcctcgt cagatgcgtt tctttcaacc
     8101 cctaaaacct catagtaatc ccggttgccc gtagcaaaca ccgcctcgtc cgcgctattt
     8161 aattgactga gttcgagtca gcaaatccga aaggctttcc gctatgtgag ccaccaatgc
     8221 tatcgtaagg gagtagtcaa tcctggttgg ccccactact gcgatggtgc ccaccacctg
     8281 gccgcgtatt ccgtaagggg cacaaatcac gctgcattcc ctcagttctt cccttgtgtt
     8341 ttcagaaccg atagtcacct gcgccccacc cagccccctg gacgcagacc ctgaaagttc
     8401 agtcaatacc aggtcatccg attcaagaaa ctccagaatc ggcctggcgc gttcgatttc
     8461 gctgaactcc ggttggccga ggagcttcat caagccgtcc acataaaccc tgttctcatc
     8521 tgatgatctg atagcagaga gcaacaactc aaacatctgt tccaggaagg atgcgtaagc
     8581 gtccaggtcc gacaggatat ccctcaacac ctttccacgc atctgcgaaa gcgaccttcc
     8641 gcgcagcctg tggttgagca tccgcgacat attctcaagg tcgacggggt tcatctcatg
     8701 ctcaatcgaa aggattttat gctccacaag acctgcgttg gtgacgacta gcaccaatat
     8761 gctatcggaa ctgaaaggaa ccaactgaat gtggcgaatc tcagccgttt tcactcccgg
     8821 acctatcact atggacgcac agttcgaaag ctctcccaac accttcgcag tggccctgat
     8881 caatgattgt atctcatcgc tgcgctttgc gcattctgcc ctgatctttt tttcctcgag
     8941 gccagtcaga ttccgctcag gcatgagaga gtcaacgtag aaccggtaac ctttatcgga
     9001 cggaatcctg cctgccgaaa catgtggctg ctcgagataa ccttcctcct ccaggtcagc
     9061 catttcattc cttatcgtcg cagaactgac tccaagtccg tattttctgg cgatggttct
     9121 tgagcccacc ggttcagccg ttgatatgta gtcatcggtc acagctcgca gtatagcctt
     9181 tttcctgtcc gccatgttct cagccatcgc tctcacccca ctctcaattt taccagcctc
     9241 tgcacgcccc aacgccacga taacccaggt cgtttagcac tcacagtagt cgagtgctaa
     9301 aaacaacata acatcacagt tcggccctgt caagactgag cactatctcc acagtcaaca
     9361 cactctgaga acaccaggtt cccaaggagg aatcctctag gagtaagtct cactttgcca
     9421 ccagagaagt tgacaagccc tttttccgcc aagtctgcga accttgcgcc gtactctccc
     9481 aacaagtctt ccccataaag acgccctatg gaagcgaggt caatcccttc cgacatgcgc
     9541 aaccccaaca tcaatctgtc ctgggcttcc tcgaaagccg ttcgctcctc gaacccagac
     9601 agcgcccgcg tcccttcgga tatcgaagca acgtacttgt ccaccgactc cacattccac
     9661 gacctgaccg atccccccgc cccggcaggc agatgcgaat gggcggccac cccaagtccg
     9721 acataatacc cgcaagtcca gtaccgaatg ttatgcacgc tttcgaagcc tggaatcgca
     9781 aaattcgaga cctcatacct tacgtaccct gcatcttcca cacgggaaac cagccactca
     9841 tagaactcgg ccaccaagtc gtcatcgggc accgtctgca cgcccgaagc tatagctcga
     9901 gccaacacag tgccctcttc aaccgaaaga cagtatgcag acaggtgctg cggtccaagg
     9961 gatatcacag catctaaagt ctgagccaat gactgaccag tctgtcctgg cgcccctaaa
    10021 atcagatcca cactgacatt atcaaacccc gcttcacgcg cccactcata ggcacgcaaa
    10081 cagtcttctg gcgaatgcac gcgtcccaat gaagctaact ctcgcgcatc caaggactga
    10141 aatccgagag aaaggcgatt aactccgctc tgacggaggg cagcaagccc gcggggatcc
    10201 acagtgcccg gattagcttc cacagtcgtc tccagatccg cagaatcgaa gcctccatat
    10261 gttggatccg aagcgttgat cttttccacc tctgcaatga gcgtgctgac gaagggagcc
    10321 agcttctgcc agggaacaca cgttggggtt ccgcccccta catatagcgt agtaatggga
    10381 cgtatctgtt ccaattccgt ctttctcagg cgcaactcgt caacgactga agacaaatac
    10441 gcctccatga cttggatatt atcacctgca ggatatgata tgaaatcaca gtagctgcac
    10501 ttgcgtacgc aaaacggcac gtggacatag actccccccg aattctggtt catagctcat
    10561 cgtcatcggc caatacagcc ataaaagcct cctgtggtat ctcaacatgc ccgaactgct
    10621 tcattcgccg cttgccctct ttctgcttct caagcaactt acgcttcctg gagacatcgc
    10681 ccccgtagca cttggcaagg acatccttcc tgacagcctt cacagtctcc ctagcaatga
    10741 cccgagatcc cacagcagcc tgaataggta cgtcaaaaag ctggcggggg atgagcttgc
    10801 gcagtctctc caccaatgac cttccccgct tgtcggcaaa atctgcgtga acgatgctgg
    10861 acagggcatc taccggtttc cccccgacca ggatatccag tttaacaagg tcagatggcc
    10921 tgtactccaa gaattcgtat tccatcgatg catagccacg cgtcctcgac ttgagcttgt
    10981 cgaaaaagtc gtaaatgatc tcggccaacg gcatgtcgta tgtgagcctc acccttgttg
    11041 ggccaatgaa gtgcatgtca tggacttccc ctcgtctttc gacagcgagt tccattacgg
    11101 ggcctatata gtctgaaggt acgaatatgg tagttcgcac aaaaggttct tcaatcgtct
    11161 ctatctgtgc tacaggtgga agatttgccg ggttgtccac cattatgcac tctccggaca
    11221 tcttgtttac ccgatacgcc acagatggag ccgttgttat cagttcaaga ttgtattccc
    11281 gttccagcct ctcctggact atctccatat ggagcagacc aagaaaaccg catctgaacc
    11341 caaatcccaa ggcagcagat gtctctggct cgtaggctat cgaagcatca ttcagcttca
    11401 gtttttcaag agcatccctg agatccccgt aggcgctgtt ctccagtgga tacaacccac
    11461 aatagaccat tggaacagcg ggccgatagc caggcaaggg cgacgccgca ggattctccg
    11521 cggatgttat cgtatcgccc acggggacat ccccgacttc ctttatctgc gccaccaaga
    11581 accccacctc gcccggaccg aggcattcta cctcctccat ttccggcctg aagaccccga
    11641 gctcggtaac atcatgttcc gttcccgtgc tcatcacctt gaccacgtcg cccaccttaa
    11701 ccatgccctc tacaatgcgg atatgggcga tgacaccgcg ataaggatcg aaatgggagt
    11761 cgaatacaag agcacgcaga ggtttgtcgc ggtctcccga tggcggagga atgcgctcaa
    11821 ccacggcctc caacacgtca agaacgccct ctcctgtctt ggcgcttgtg aagatgactt
    11881 cctcaggacc cacgccaaga agatccacca gctcctcccg aacccgttcc ggctcggcat
    11941 tgggcaagtc tatcttgttc accacgggca ctatctcgag gccattgtcg accgccagat
    12001 acagattggc cagagtctgt gcctcaatcc cctgggttgc atcgactacg aggacggccc
    12061 cttcacatgc agcaagggaa cgcgatacct cataggtaaa atcgacatga ccgggagtgt
    12121 ctatgagatt gagcacatag acctccccgt ttgggcgagc gtaccttatt cgcactggtt
    12181 tcagtttgat ggttatcccg cgttcgcgtt ccaaatccat tttgtcaaga aattgctcaa
    12241 ccatggaacg ctcatcgatc gcgccggtca gttccagaat gcgatcggca agcgtagatt
    12301 tcccatggtc aatgtgggca ataatgcaaa agtttctgat accttctatg ttcatatcca
    12361 cctcagagcc actatgcgcc gcttttcgcg gcctggtagg aactccacac taaattataa
    12421 cacatgtagc aatgactctt cggagacatg ctcgacaccg attgcccgga cctctcatac
    12481 tacttcagtg cattctcaaa ttcgatcctt acggctcgtc cgaaagcttc gagcactatc
    12541 ccatggtaac ctccgccaac cgcccgaatc cggacggggc tccactggct accggctccc
    12601 gccagctgag acgaattgct gcccgccact gcgatagcta tgaacacaac tatcaagaaa
    12661 aggcatgcaa tggcagggac caccgcaaac gcccggcggc ctctccccgt ttcccactgg
    12721 tcgattctcc tcattggcta gatgatcctg gccagagccc gcgcaacgag cctggccgat
    12781 ctgcaagcct cctctacagt gttatccgtc cctccaattt ccaagatgat ggcgcccgga
    12841 tggacgtgct ggttgaatcg ggcgttcttc acgatagcaa ctcccctgga aaccccagga
    12901 tacatcttat ccatctcggc agaaagctcc aacgcgaagt ggtagttctt cctccaatta
    12961 ggatgtgcaa gtcctgagct gtcgtcagtc acgacaaaca gaacacgcgc caccttttcc
    13021 ccgccgacca tcgccgtttt cagtttttcc cggttagcgg gcaatgaatc acgatggata
    13081 tctacgaccg ctctgaggct agaatacgac ttcaccaaag acgtcatagt cttcagtgac
    13141 ttcgagtaac tcaaagtcca gtccggccag tcgtgtactg ttgacgcgtg aattgacgga
    13201 acccccagaa cctccgacag ctcttcggcc attgccgcgc ctactttcac taccccatcc
    13261 ggttttcccc aacagtaatc ccttcctgac gtcgcccgat aggcttctga tgtgtgcgta
    13321 tgaagtatgg ctatcttagg agatgccccg cgcagcacca tattgtcggc aatctccctc
    13381 gcagccaact gcggaaactt ggacggctga ctcggcaccg gttccctcgt cggaacacta
    13441 ttcgcaccct gtgaatccgc gtctcgccca ggagacgatg tattgctgga actctccaca
    13501 acaggtttgg aaaccgcggg cacagctgca tttgcagact ctgatgaaac tatgacttga
    13561 acggacgtgc catctgggac ttctgtgcct gcctccgggt cggatgagga cggctgatct
    13621 ccgaagacca agagcccaga gctggtcaac gactccaact ctgcgtcaga caactggccc
    13681 gacaccaggg tcgaattgga cacgagagag ccttcggcgc gcatcggcgc agaaatgtcc
    13741 ggatcctcac cggaacccct tccatatgtt gcggagcgcg acgggttgtc cgaggtccgc
    13801 tgtgcggcaa ggatggaagg aagttcggct cggattagag tacgtgggtc cgcaaagtcc
    13861 agccctccca tcgctctaag tgccgcattc accaacctcg ggccgatcga gccgaacctt
    13921 tcctcagcgg atcgattggg aagactccgg tcgctacttg gccgaaacag gctcaagaag
    13981 ctcgacacca tcttcgaaac cttgctccac aaagagggtt cagaggcgag cgtttcgcca
    14041 tccccgacaa catcctgccc cggcacgccc gagacagctg gcacagaaac gccatctttg
    14101 actgcacgcg tgcctattat gaggccaaca gccgcaaagc agaggatcac gtagaatacg
    14161 aataggccgc ccaacggccc aatcttgaga ccgccggccc tccttgattt ccgtctcgtt
    14221 ccgcgccctc gctgcaatgg gatcacctct ttatgcagct gtatgcgcct ggcccatcag
    14281 gtattcatac cccctcattg caggtatagg tatatctcgt cctcatctat cgcaggatgg
    14341 agcgcgtagt taatccctcc agccaccacg tgagagagtt ccttgacgag cacgtccacc
    14401 tccttgggag taactatgag cgtcccgaaa tatggttgga gcatctgcgc caccgcagga
    14461 ggcagacccg gctgccccac ccccaattct gacagaggac ctgccgcagg cctcccgacg
    14521 ccgggctgtt gcgagctacc gacgatttcg agggcatccg agattatcgt gagcgcgtgc
    14581 accacagttg gaacccccac agcaattaca ggaatcccca tgctctcacg gttaatccca
    14641 aaccttctat tgcctacacc agatcccggc tggatgccgg cgtcggaaat ctgaatcgta
    14701 gttccaagtc tagacacgct gcgcgcggcc agggcatcca cgacaatgat caggtgtggt
    14761 ctaacccgtg ccacaacgcc cgcgattatc tcggccgtct cgactccggt caagcccaga
    14821 actcccgggg ataacgcagc cactgacttg agcccccctc gtttttccgg cggagtaagg
    14881 gaatatacgt ggcgagtgac taaaacctcg cctgccacca tcgggccaac agcatcaggg
    14941 gtcgcgttcc aattgcctaa cccgcatacg agagtcgtga aatcggggac cgtccaaaaa
    15001 tccacattgt taaaattggc cctgatcaag gagaccaact cccgggcgag cgcctccgac
    15061 acgtcctcct gcaatccctt gtcacgcagg gggagctcag gggcgtcaat agtcacgtaa
    15121 ttccccactg cctttccgag agaccgcgca gcttgctccg tctggactct cactcgcgtt
    15181 attgacccaa agtccatctc ctcaacttgg gaactgactc cgggcacctg ggcccggctc
    15241 gccctttgta ctagttcgtt ggcctcaaca gccaggtccg tcaagtagcc gccaccgctc
    15301 agccccatgt cgcattctga aagatgttgg agctctttgg acggacgcca ttttgcgccg
    15361 aaatttcggt caaaccccaa tcctaatgca cctcctcggg atgcgcagtc tgtatctgca
    15421 catgcctagt ctttcccact cggaggcccg ttcatcaatg tctgctggca tcttccccaa
    15481 gatccttcaa cgaccgaaac ctctcccgac cgggctggag tgccattgcc ggactcagcc
    15541 tataatcaag gcctccggaa acgacgtccg gctcaattcg tcgatttcag tattgaggtg
    15601 ctaacatggc cacagggtac cctcgctgtc gagttgtcag acgtcgcaaa tatcaattct
    15661 tcccgccaaa gacaacacat ttcggatgaa agctccattt ggaacgacgg ctccttatca
    15721 acgaaggact catcttaaat cagccaccat atactaacct ccagaaacgc tctggaattg
    15781 gtcttccacg ggcagactcg cttgaagcca gataacagca tatttcacgt ctccgcaaga
    15841 atgccattct caacagccga ttccgtcaca acttgccctt attcccctct catctggatt
    15901 tacttgcaat gacagaacgc ccgtggtaga atcataggcg tgcgatcaga tggcggaggg
    15961 ggaggtgact gagagtgcca aacaccagat ctgcaaagaa gcgggttaga caggctagga
    16021 cgagaacgct gaggaatacg tcggtcaagt cctccgtcaa gacagctgtt cgcaagtgcc
    16081 acgaagccat ggacgaattg ggacctgagg cctgtgccaa ggagcttaat cacgcgtaca
    16141 gcacgattga ccgcgccgcc aagaagggcg taatccaccc taacaaggca gcgcgtctga
    16201 aatcaagcct tgccacaaag gcagtcaaca tgggaaaagc gcctgaagcc ggcaggtagt
    16261 ttcgcataca aaacgatagc gcagaacctg cgccacccgc gtcgtccggc tattcagttt
    16321 gcagctccag taacaatcgt tgctggagtt tttgctatgc gcagatttct gcgatcaaca
    16381 cttccatgag cagcctgtca ggcatagtcc cggtcttgaa atcgagatct gctcgggcga
    16441 tgcgcgatat catcctggcg atgtctgcct gtgagagctt tgctgcttgt ccaacggcct
    16501 tgcgcgctgc ccaatcagct tttccggggc ttctcgaaat cgcccgcgcc gcctcgtcac
    16561 cggtcaaacc acggtcgagc tcgcatctgg cggacatcac acgccgaacc tgagtggcca
    16621 gagtattcaa tatgctcaga ggatgcctct ccgtgctcat cagatcatcc agagcgcgca
    16681 tcgcccccga acagtctccg ccgatcaccg catcacagaa ctcgaaaacc gtctgcgtag
    16741 gacttcttcc cacggcagtc cttatgtcat ccacggtaat gcgcattctt cccacgcttt
    16801 tatccggccc taggtatgtg atgagcttat ccatctcctg cgtgatggcg ccgtagtcgg
    16861 cgcccacaaa ctcaagaagg agctccacag cttcaggctc gatttccacg ccaagctcct
    16921 tcgcacgaga atacgcatac gatctggcat ccttcagata agctggccta aactccactg
    16981 caacaagatt gcctccagct gcagctaccg ccttccgcgg caagctggtg gaattcgacg
    17041 tgaagatcac acatgtgcct tcaggcattt gaaggctcgc gcgatccttg ccaggagcga
    17101 taagggatat tatcctgccc tgctgggccg cggaaatctc ttcgaatctg cggaccacca
    17161 ctactctgga tgacgcaaac attggaacag tgagtatggc tgtgacgatg tcatccacag
    17221 atgactccga catatcgtag acgttgagat ctgcgcctcc ccgggcagcc gcaatagctt
    17281 gcgcgagctc atctgcattg acctgccggg agtatgagtc ttcaccgtat aatatgtata
    17341 tgggcgcaat tcgcccagcc ctgatcgcct gctcaagatc tgctctcaat gatatccccc
    17401 tcatactcct tgcaaatcgc ccagtcgaaa ccggaatctg atccgtcccc gccgccttgc
    17461 taacccttta agcctgtgcg cgctatcccc tcgatcagct ggttctgggc tatgaaaaag
    17521 attatcacca atggcactat ggcaatcgta gaggcggcca tgagcaagtt gggaacagtt
    17581 ccaaactcct ggttgaactg agaaagccca acctgtatcg ttcgatatgt cgaagaattg
    17641 gtgacaatca gaggccacag aaacgaattc cacccgccga taaacgtaaa cagcccgttt
    17701 gttacaaata ctggcttcga caggggaact atcacccgcc acagatactc gaatcggctg
    17761 caaccgtcga tttgggcagc atcccacaac tccataggga tggtctgaaa gaattgcctc
    17821 atcatgaata tcccaaaagc gctcgcagcg aacgggacaa taagggcttt gtatgtgtcg
    17881 atccagcccg ccttggctat tatcgcgtag ttcggaacca acgttacctg gctcgggatc
    17941 atcattgtgc caagtagaat catgaacaca gtttccttgc ctgcaaaatc gatcattgca
    18001 aatgcataag ccgcaagagc cgacgtgact agctgcaaag ctgttgtaat agtggcaatg
    18061 aacgcggagt tgaagaaata ccgcggaaaa ttgggatcag cattccatgc ttcagagtaa
    18121 ttgctccata caaacctctt gggaaaccag acaagctcag gcgtatacaa ctcctccggg
    18181 cccttcaccg aagttatcag catgtagtag aaggggagaa gcattatcac cgctcccaca
    18241 gtcaggagga tatatacgat cgacattcca acagtttttc tacgtttcga caaactaatc
    18301 tccccccgac tattggtagt gcacgcgctt gcccacaaga tctttttgga caagcgtcaa
    18361 cacaaagatg atagcgaaaa gaatgtaccc gatcgccgat gcatacccaa acctaaaagc
    18421 cccccaaccc tggtcgtaaa ggtatttgac gacgacccga gttgttccga gcggtccgcc
    18481 cgatacagat ggccataaca catagacctg ggtaaagact tggaacgatc cgattatcga
    18541 caccatcaat atgaagtagg tagtaggcga cagcaacggc cacgttatgt gggcaaacat
    18601 cgaccagcca cttgccccat ctatctccgc cgcttcgtag tactccgcag gtatgttctg
    18661 caaaccggcc aggaaaagca cgacgttata acccaagtgc ctccagacgc tcatgaggca
    18721 tagagcgaac atcgaccatt tgggatcgag caaccaggcc tggggttctg ccccaaaaag
    18781 gcacagcaag gagttgagca tccccgcaga gtcttcctta tatatccacg tccacacaat
    18841 cgaaatcgcc acaataggag tgacataggg caggaagtaa gcggtcctgt acgcggacaa
    18901 tccgcgtatg ggcttgttga gcaaccacgc aatgagcagc gccaacgcca tacccacagg
    18961 gacggtccca agcacgtatt tcccggtgtt caccagggat tgcagaaaat cagcatcacg
    19021 gaaaagctcc gaatagttac caagccccac ccaatcccat ctgcctatga gattggtgtc
    19081 gaacaagctt atgtagagag cgtagaacgc gggaaacaag ttaaatacga tgatcactat
    19141 caacgccggg gcaagataca tgtatgctgc gacagtatct ttgacatatc tcttcctctc
    19201 caatgaattc attgcctgtt ccactctcct ccggcaagcc aagatattat cgacaacaca
    19261 aacgcaccat ttccgcaacg ttcccgggcg agatcgcgcg acgaatagac gggcgccccc
    19321 aggcagacca gacggcctga ccccacagtt tcggcagccg caaaaacagg cgacccggcg
    19381 tattgaaaca gccttccctc gccggagaca tctgccgacc aagcgcccgg cgcatgcgcc
    19441 aacaccacta cttggggcac ctcactaaga tcgatccagc cagggcccgc cagcgagcac
    19501 gcttcatcgg catatacagc acgctccagc ccccattcca catccaagac agacagatca
    19561 tcccgcctca tctcgtccct gttgaatctg atggccgatc ccatttgctc cagcaacaga
    19621 tcaaactgat cacaactgcg caagccgggg aaatcggacc cgcctgccag aagcagagac
    19681 ccgcccgccg caacaaaact ggaaattgca tcgagctctt catcatcaaa agacgtgggt
    19741 gtcagggaaa acccggtttc cggcacgttt attacgagta tgtctgcacc atctagagtc
    19801 tgggcgtcga tgcgtccttc atttatccgc ggattataat cggcttcgcc caggagtctg
    19861 gcaaatgcat ccatatatcc cgagcagcga ttgttatgcc cttcatctat taccacgctc
    19921 ttgatgtctg aactcctcac tctggcagac ttgccacagt aaatatcgcg tgccccttcg
    19981 ccgcaggcaa caagctccag aacgaaactg acatcccccg ccttgctcgg cgtccattca
    20041 aaagatacag gcatgccgcg tcctgcggga aggtccactt gggcagagga gatgaacgtg
    20101 ggcgctccct cccgcatctc atacaaaagc acagtggcgc cgtgtattgg gacatcgttt
    20161 ctgttcacga tctccgcaca cactcgcgcg ggattcccct ctctcggcgc aagatcaacg
    20221 cagcccagat cagacacggc aatctgtgaa ccggttgaaa cccagatagg gctggtgaca
    20281 atcacatcca tgtccgcctg gaccgccctc acataatacc agttatagct ttgagccggc
    20341 attatctcca catctgctct gaaaaccccg gtcccacaca cttcgcgctc ccacaccacg
    20401 acgccaccgt ttgtgattat ctccaccgat gacaacatgt catcttcatc gggatcatct
    20461 atgacgattt cgaaatacac agggcgcccc gaatctatgt cgtcccgcga gatgattata
    20521 tcgcttccca tctggcagtc gcctgaggtg aaggatacgc gaacattcga gtcctccgtg
    20581 gcataagttc tcatgcttcg aagcgactcg tatatggcct cccgggtcaa ctcggaggcc
    20641 aagaccgctg tacgagtggg agccgctgtc ccccagtccg ccttgtgatt atcttggcac
    20701 gatatggctc caacatgcca tcctcgatca agagctcgac agtatgcccg ttcgttccgg
    20761 atgttgtgtc ggtacgggcc tgaaccaact tccaggagac agacgaaacg atcaccttca
    20821 ccgaggtatt cgaaatcgtt ccagttcggt tggatatcgt agtttggatg attgaaaact
    20881 ccgattccat tgtaatgggc aagaaagtcg atgaaagcgg ccagattccg ctgcgtatcg
    20941 cgggacgcag cgagaggagt atccagcccg ttcatatgcc catccgtgaa tgtccattcg
    21001 aatccgatca tggaaacgaa tacgccgggc tgataaacct catccgcttc ctccaagctt
    21061 ttgaaccaat gatggatatt cgtggcttcc tgcagatagt agccgtgatc agtaactgct
    21121 aagaaatcca tcatcgcaac atcccttgca tgccgaaatg cgtccgacgg cgtgagaacc
    21181 ccgtcggaat aggatgtatg agagtgtagg ttgccaaaat agcagttaag ctcgacaaca
    21241 ggctccgccc ccaagcagac gcaacctgcc attaccgaaa agagcgtcgc ccagaccaac
    21301 accagtctac gcctactaat aggctgcttg tgcatgatag acaccgcttt cacaagattt
    21361 gaagcccggg gcgcacaggc attgcgcccg acgccccagg catccgtaga ccctattgcg
    21421 aagggacaac cttgtccgag tcgctgacga tcacagaata ctcaaatcca ttgcgtcccg
    21481 gctgcagcgc ccgggctacc tgattcttga gttcgccttc gctgccgcgg catcaagagc
    21541 ttccttgggt gtagcctttc ccaggaaaac cttttcaacc gcttctgaca catcgcccct
    21601 tatgttgttc catgcagcca tgttcgggtc gtatttgacg taacgcagct gctgcaaact
    21661 ctgctcatcc cttggatgca ccgcaaacca ctttttcatc atgtcaagat gcattgcaga
    21721 cttgcggaca ggaatatagc tggtgcctat tgcccatttt gcggtctgtc tgggctcgac
    21781 aagccacttg atgaatttcc acgcagcctc ctgctcctca ggagtggctc ttgagaagat
    21841 tgcaaggtcg gtgcctgcaa caggcgtgga tctgaactta gggtccatgt acggcaaagg
    21901 tgccgcgctc cattcaaact tgccagcaac agagttctca gtatagctga gccctggtga
    21961 cgtcgtgaaa tacatggcta ccttgcctgc tccgaagtcg gcatcgagat agccagggac
    22021 atagtatgac actttgtact tattgagcat gtcagccatg aactgaaggg ctgtgacgcc
    22081 ggcgggcgaa ttgaaagcta ccttcccctg cggattgagc catgaccccc ctgcgttaaa
    22141 gaagaaacag gcgaatatgt cgatgtttgg cctgagacca aagccgtacc tgacaatctt
    22201 gtcgccatcc ttcaccgtga gcgccttggc ggtgttaacg agttcttgcc aatttttcgg
    22261 cggaccggaa atcccggcct ccttcagagc ggtctgattg tagacgagca catacacgct
    22321 cttattaaag ggcatcgtat accaaacccc gtcaaacatg cagccctctc tgaggccatc
    22381 ccatatatca tccacttcta gctgggaaag gccattggga cctttgacaa acttctgaat
    22441 cggaacgagt tcgttgccgc ttatgtactc agctgcccag tttccgtata cttgcgccat
    22501 agtaggaggc ttccgcgcca ccagggcacc gacgatcttc tggctgagcg cgccgtagtt
    22561 tccttgatac tcagccttca cgtaaacatc aggactctgc ctgttgaact cctcgacaag
    22621 tccttccagg gtcttgccta gctgtgcccc catagcatgc cagaaagtaa tcgtcgtctt
    22681 cccggccgcc attgaaggac cgacagccgt agtagcaagg ataatcgcag ccatcagcgc
    22741 aatagtcgca aacctcttca atgcctactc cccgtactgc ttcagcaatc ggggaacgac
    22801 ccccctctct agttttgacc ttgcacacct agttttcgac gaagaggtct ctactcctgc
    22861 tgatttaagt ctatttatcc aacgtctcaa gctttctgaa cattggcgca gtttgccaca
    22921 gagccctcat atcgctcaca gtcagcctgt gggacttagg atgccattcc actctcacag
    22981 cgcccacttc atcagtccgg agaacagctg cacccgactg cagaagcctt tccactgtat
    23041 cttgcgacgg atggccatag ctatttcgcc ctaccgatat caccgcaata ctaggcttca
    23101 cagcctccaa aaatcttgca gaagtcgcac tggacgatcc gtggtgcccc accttgagca
    23161 catcggccct tagcacgcca tgtccctctg gccccaaaca ccgaaacact tcttgttcga
    23221 atttccgacc cgcatccgcg cacaaaagca tggagaactc gccgcacaca acgtgaaaca
    23281 caagggaagc ctcgttggga tcaaacgtca ggttgcccga gaatccaacg tctccttcca
    23341 acgaagtgcc gcgcaaccca tcgcccactg cttccggacc accaccgtac acgatgggat
    23401 tgatcatctc gagcctcaca gatcccgcgt tgatctgatt acccctaacc aactgtgcaa
    23461 gagacggcgc attgctatac tggcactccg accctaccaa tatgccgtcg atccatgcct
    23521 gatccatgcc tctgggaatc attaccgtat ccgtctgaac cttgcccgca agggccgcaa
    23581 agccgcctgc gtgatccaaa tgctcatgtg tgatgatagc cgcatcaatg tgcctcttcc
    23641 cacagcgctc aagatacggg agcacatgcc tttccccggc acgctcagtc cccgcgtcta
    23701 tcagtaacgt atgcccttcc ggcgtaaaga gcacggcagc atccccctgc cccactgaaa
    23761 gaaaaacaac ctcgagaggc cttggccacg cgatgaccac gcccaggatc agtgcagcga
    23821 tggtaagaac aatgccaagc ttgtcccgac agatacgcga tagccgcctc gcagccctga
    23881 acctggcaaa acggtcccac gcaagccacc agatgaccga gaccatcaga tagtatgcaa
    23941 cgagttcgaa gcttcgcaac ttcctacagc atattgttgc gccaggaagt gatgacatcc
    24001 atccgaccaa atgtgtaagc acccgcaaaa caacaccgac gcccgtaatg attgccgaac
    24061 ccacccaggg aagaaagggg taaattgcgc aaccaaccac gcccgccgtc atcacgactg
    24121 atgcaagcgg tagagccgcc agattggtta acgggcccac gaagcacacc tggcctgtca
    24181 tgttggcaat tatgggccaa gtcgctatct gtatgcccac agataccgtg agaacctgga
    24241 taattagcgc agacagccca agaggcgcat tctcccgcgg tcttgttgca aagggggcaa
    24301 aggcagcgag agcacccacg gcgaggtagg acatttgaaa ccctgcattc caaagaagca
    24361 aagggttgat caagagctga acggatgcgc caatggctaa gggatggatg aggctgccct
    24421 tccttcgcct ggcctttgct aggaccgcta ctgtcagcat caaccatgcc cgcacaacag
    24481 atgcccgcag gccggcagcc acggcataga tgccgccaaa caagaccgcc gcaatatcgg
    24541 cggcttcggg cgacgcgcct acaatgccga caagagctcc aaaaagcccg gcaacgaggc
    24601 caacatggag gccggaggct gccagcagat gggcagcccc agatttcctg aagctctcga
    24661 gaataatggg atcaacccct ctgtagtcgc caaacatcat ccctgcgagg agatcccgtt
    24721 caggcggatc gagaaccgca ctggctgagg cgaccatctg ccgccgcact gcatcagcca
    24781 ccaggagcaa cgggcgccga ccattgcgtc ctgcaaagac catcgaatca tgctctgccc
    24841 atgcaacggc atgtatccct tgctgcaaga gtatcatcct gtaatcgggt tcgccagggt
    24901 tcatgggacg atcagcgtat tcaacctgaa cccttgccca cacaatgtcc cctacgcgca
    24961 catcccaggt aacccaatcc tcctcatgcc cccaccgggg ccttatccga acagccccaa
    25021 ctgccgagcc atttgaaccc tggtccatga ctgtgtcaac atcgaggata agctccgcag
    25081 gatccacgta cggccatacg gatcgtatgg tgccacgcac catcacatcc cgccccgcat
    25141 agcgcgcaat cgaccttgca gccatcccat ctgtacgctc catggcaact gcgcccaagc
    25201 ttattccggc caaggccaag accgcccagg ccagcacggc tcgtcttttc tttgataatc
    25261 tgagcgtttc aattcgggcg aacgcaattc ccgccaaaac aagccatgcc caccgcaaac
    25321 agaagccggc ttcacggaat cccaagagta tgcccacaat caggaacaag gcaattactg
    25381 gcgccacagc acacgctccc aagtttccgt gggtgaccga tgttccaaag ccaaagagat
    25441 gcttcccggg attctcgttt cccgccgtga gcgccattta cctcaccgtg gcatggggag
    25501 caatcttgct cagtttcgcc tcgccgatgc cggagacgcc gagcaaatcg ttgacggaag
    25561 aatacggtcc attgatcttc ctatactcta caatcttggc agctgtaact ggaccaaccc
    25621 ccggcaattg ctcaagctcg gcgactgaag ctgtgttgat gtttatcaat gtttccttgg
    25681 gtgacaacgc cgagatatct gctctattcg aaggaattct cgcctccgaa gatccggatg
    25741 gcgtaggctc tttgcgagga atacatatct gctctccatc atgtaccggc agcgcaagat
    25801 tgaccatatc gagatcggcc tcagatgttg ctccccccgc cgccctaacc gcgtcggcaa
    25861 cccgcgagcc gtggggaaga gagtagactt caggacgagc caccgcgccc gcgacatgga
    25921 ttacgattaa gtcatcgtcc aactcaggcg ttaccgaacc agctacggga ccctcgacgt
    25981 ctgtctcgca cgacaggcaa tccccgccat gaatggatcc gtcctgatag gaggaatcag
    26041 tctcctcatt ctcgatcacc accagtgccg gtcgacgcgc ctgtttcaag gcaaccgccg
    26101 ccagccctaa caagcccact gcggctacaa tcgccgcaat cttccgtgga ctcagtttgt
    26161 tcattgacaa tcccccttga aagcgccgac cggtcatggt ggaaacgccg agctgctaga
    26221 ctcatgttac cattcggaca ccaccgccgc ctcgcccttt tgggtgagcc aactgggtga
    26281 tttttggcgc caaagcgtcg agaggaaaga tgccgacctc gtggtccttc atctcgccac
    26341 gcctcctttg ccatcaagct cacatggcta cagtctcttc ccaaagtcct ccggagcgtc
    26401 tctagctcaa aggcaatctg ccccgccgaa cttcgcttct cgactgagca gacgcagcgt
    26461 ccagtaggct cgtaacaccc ggctcacccg tttccaccgg catttcagcc cctgttatct
    26521 ttctcccata cttgcctaca gctgcagcaa tcaccgtgaa gatatggggc aacgtggctt
    26581 ggagcaacat atgtcaagcc tttggttcca aaactcggta tttacatgag gcccgaattg
    26641 ggcaaaaaga gtctcctacg gtcatcaatg gagccatagc actacgctta gttggttgag
    26701 ctccccccta gttttagcac ttctgcagag gctgacagat gaggagcccg aggctttgat
    26761 ccatgccttc gttgccgagc ctacatccga aacggcaaca ggatgctccg taatcaagca
    26821 agtcctgcga acagaaccca aagctgaaag accttagtcc ctccgacctt ttatagcaca
    26881 tctgcgggtt gtcttcccgc tggttg
//
LOCUS       DAOSYL010000029        25934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1559_length_25934_cov_8.129139, whole genome shotgun sequence.
ACCESSION   DAOSYL010000029 DAOSYL010000000
VERSION     DAOSYL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 25934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25934
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1559_length_25934_cov_8.129139"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLU15_06330"
     CDS             <1..193
                     /locus_tag="PLU15_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30575.1"
                     /translation="GGAWRALTDGRMNVRPRALIQARLRAYRISPAGFWVYVLSVGLY
                     QLMGFAFVVATTSRPISVL"
     gene            complement(292..669)
                     /locus_tag="PLU15_06335"
     CDS             complement(292..669)
                     /locus_tag="PLU15_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30576.1"
                     /translation="MAIPSIATVGGYRGAVVYQYLKNRLIPYLKIALEPGRARGPHLM
                     NPGIKCHGISANPASTRQAGALATTARGITQVKMMRIASGQKRIGYCPNCTRSIARLI
                     RAERPLLYRTSEAAITKRLYTED"
     gene            complement(788..1963)
                     /locus_tag="PLU15_06340"
     CDS             complement(788..1963)
                     /locus_tag="PLU15_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOO30577.1"
                     /translation="MSEEQQTRRRTPAQLRRSALIGVVFSVLGLYVVLQFTGGRTSWK
                     EFAQIDPAMLAIACGLVIVTWLFDSLRMRALIRSLGGDLDILTGMRISIMGAFVSNVT
                     PFDSGGEPMQAYLLTAKGLNAGQSTAVIAVKTICNALARFTLGIAASIWLFGFADFWS
                     IPKAMYVLLAIGIILYFVVFAFSLYLIFHPEKINVIVVPVVRNKFTLKFFKPETLDAV
                     LDRIDHELREFRNALQEFMENKKSALWKVMWLSYAWWITITIVPAVILVGLGITPKFT
                     HIMGITLIFYLAAAYAPTPGSSGAAELGFSLLFSSVVPHGLIGLFVTVWRAFTYYLNL
                     IVGGVLMAVGMIKKNMPSEKQGRSDEIPQPQSEARARESDTISTPSSGAAHTIPPAS"
     gene            complement(2279..3226)
                     /gene="arcC"
                     /locus_tag="PLU15_06345"
     CDS             complement(2279..3226)
                     /gene="arcC"
                     /locus_tag="PLU15_06345"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOO30578.1"
                     /translation="MDNAKTIVIALGGNAILQPGQNGTVDEQMENVDSTAEQVAELIA
                     QGHRVVITHGNGPQVGALLIQQEAGRDRVPPMPLDVCGAESQGQIGYMLQQSIGKMLA
                     RRSIPRPVATVITQMVVDPNDPAFANPTKPVGPFYSAYYAEQRMKETGEKWIEDAGRG
                     WRRVVPSPDPIRIVEQDAILSLVRTGAIVIANGGGGIPVIEKGGLYKGVEAVIDKDLG
                     GECLARDVGADVLLILTDVAHVALHYKTPHQVDLHTVQLDDLIRYQQEGHFKAGSMGP
                     KVEACRRFVERGGEAAIIAQLDRAVEAIHGNAGTRIVKQ"
     gene            complement(3293..4480)
                     /gene="nagA"
                     /locus_tag="PLU15_06350"
     CDS             complement(3293..4480)
                     /gene="nagA"
                     /locus_tag="PLU15_06350"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOO30579.1"
                     /translation="MAHKTLITGGPVIAPTSVIANGAVLIEDSTILNVGSAASLGKET
                     VDATIDAKGGIIAPGFINLHVHGGGGADAMDSSFDSLNCMSKFEASHGVVGFLPTIMS
                     SPLEQMTGACKAAAEAIDADRLGSRCTQGARILGINVEGPFLNSIRKGAQPEEGIMAP
                     NISVLEKLLDAGGGHIMAMTVAPEIPGAIDIIHALASQGILVSAGHSDAAYADMERGV
                     AAGIRHVTHTYNGMRGLHHREPGVVGAALTIDDLTCELIIDGVHVHPIAASIVVGMKG
                     AAHVVLVTDSMRAAGLPDGDYTLATQHVIVKEGQARLESGELAGSTLAMDVAVGNMVR
                     LVGVPLPDAIAMASSTPARQLGLDDRKGRLQRGMDADITILDANTYRATTTISMGKVI
                     FNA"
     gene            4830..5924
                     /locus_tag="PLU15_06355"
     CDS             4830..5924
                     /locus_tag="PLU15_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO30580.1"
                     /translation="MSKLRVGVIGTGMAFERLHYPAYERLSDVYEIGALCDQDAGKLA
                     DWGQRLRLGAESLYTDWRKMVARDDLDVIDIMVPIELNYAITERVARELAGKRKAIIC
                     EKPLAGTFKEALSARELAQRYSVLIMIAENYRYSEEMNIIRDLVVSRRVGDPVYFIYN
                     RTVDFPSDMWKDDFAAREWRQYPEFPGGAIADMAVHDLAAMRHIFGAIDRLQAFGRPQ
                     DAEFSPYSVIVVNMLFASGMIGQFSFFCSGKEMQRPLVGFRIFGTEGMIYLEDRNSGV
                     INVAYNDRRSEQIPYDPQKAYYNELLNLANALRGKEPVSVPPEMEYGDLKTVRDILKS
                     IQEQKIVTVDKTADYLPDYGQPGREGLWLQ"
     gene            complement(6124..6357)
                     /locus_tag="PLU15_06360"
     CDS             complement(6124..6357)
                     /locus_tag="PLU15_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO30581.1"
                     /translation="MTEKKWKAVSTKPRTVTIDKAAEVLAVSTTTIQKMMRDGRLHGI
                     KRGRDWRILRSDLEPFVADAGSHKSAPSNKTER"
     gene            6524..7174
                     /locus_tag="PLU15_06365"
     CDS             6524..7174
                     /locus_tag="PLU15_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOO30582.1"
                     /translation="MFKARYLPLLLLALIFIMSIFVYRVLPDRVPMHMNWKGEIDRYG
                     SKAEGAFFFPVVILIVHLIMSLVFRAYINSIDADARSVAADRLGIFKSLLILLFAAMY
                     ADFVMVALGRPSSMYEFLGIPVALLFYYTGVLMKDTPRNGIMGVRTKWSMADDEVWVK
                     THHMASTVYRVSALFPLLSLAWPNLFWLWLAVPLILAPTVMIFASRRYYMELVGRK"
     gene            7435..>7767
                     /locus_tag="PLU15_06370"
     CDS             7435..>7767
                     /locus_tag="PLU15_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30583.1"
                     /translation="MQRRLGICGPKTSSLERHIRLLEEAISPSTPREAVEKWAETRNW
                     ALQYAVIYPELREVARCLVVFDSSTSTGPTVARISVERDKNRARLSRHMSEERWHVVK
                     TSVKPESDC"
     assembly_gap    7768..7867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8110..9516)
                     /locus_tag="PLU15_06375"
     CDS             complement(8110..9516)
                     /locus_tag="PLU15_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO30584.1"
                     /translation="MKKGMATIGTVLCSIIIVVLALTLVSTTWANSGKPGADSAAGQD
                     ALGQNALAKYVIYINWDACAYRYYEWANSPGQPGTPNLNYLASRGALFTNARNGIPGI
                     TNPMQTSIVTGTWPAVHGNTYRYYDPVENLVKETGRHNDAETIAEVVEKSGLPCASVQ
                     QFMLQERGTYTDDPYHLYIQPGAGWLKRVAEAEKILNLKPVFGMNGKMITPAERPTFM
                     AIYADDLDAAGHNASPGYGFSVARDYDSWKAKMTKLMIDMDEGLGNLIDALKKLGIFE
                     ETAIILTADHGMTPYWGPSSLQDLIKTVESLGYKCEFLGKNERASKNTDIVLVSAGLS
                     VQFYFRNAISDAQYKALIDTLASKPYVGGYFTADDLKKEGAHPSLGSLMIWPEPPNHF
                     HTSGKLIGLGGQHDSGEESSRHVFMLISGAGVKQGVVIDSPVQIIDAAPTMSRLMGLR
                     QPANATGRVLYEALVVDE"
     gene            complement(9664..10776)
                     /locus_tag="PLU15_06380"
     CDS             complement(9664..10776)
                     /locus_tag="PLU15_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOO30585.1"
                     /translation="MKLKRTADSALAKKLNRDVVIDCIRKYGTLSRADIARLTMLSPS
                     TVSSIVAELVESGIVREQKGTAASSGGRRPIMLDLDASASCAAIIDVQVSRVLYGVID
                     LAGNPVWTARDKLDLTSPEDTYHQIFQIVAKTLARLPRSGGQAAGIGVSIPGIVDTTT
                     GTVIKSTRLRLKELPLAARLQQEFSLPSYVERDVRAAAWGERSYGLARSADDFVYVMV
                     CEGGLGAGLILGGKPYAGARMQAGEIGHMTVDIHGKQCSCGNRGCLVTLTYPAIDEVR
                     RNNESDSIVDCAAIGIVNLMNLLDPELVVLGGDIGQEPQFVSQVETVVRDRMVLTPRK
                     NVRLVPSQLGDMGIALGLAGLVFDKFYWSRVFEDVG"
     gene            11219..12085
                     /locus_tag="PLU15_06385"
     CDS             11219..12085
                     /locus_tag="PLU15_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30586.1"
                     /translation="MSNKKTGTIVATAALAAIMLLASIGVSASAADIFAGWSEPMLWE
                     HSGGDTERMPYLAGNDLYFAKDYDIYWSTFDETTGKWSEPEPVPGPINTAANEACPCV
                     VGNGKVLYFSRYDPITDYDFYRSEWDEEKAEWGEPELIEELSTDGQDWAIHVNEDETI
                     AYVISKIPYDEATVVGGRDIWKSVKVDGVWQTPVNLGEPLNSNGEEWSIFVDYMGRFW
                     IDGNRADSLGNFDIYVAENEKATPINLGAPINTDVAEREPTINEKFFFFSANKREGGH
                     GGWDLWYMVRLP"
     gene            12233..13264
                     /locus_tag="PLU15_06390"
     CDS             12233..13264
                     /locus_tag="PLU15_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO30587.1"
                     /translation="MHTVAIWGAGTMGRVHAKRYAMTGEAQLKYIIDNDLEKATRLAQ
                     PFDAVATVDPGPAVRDPQVDVIDVCLPTYLHRESVELAAGHGKHVFCEKPIALTLDDA
                     NAMISTCKSNGVKLGIAHVVRYFPEYEMARDMIASGRLGRVGVVRLSRTGPFPGGWND
                     WYADPQLSKGLFVDLSIHDFDFLNWCFGPVERICARKVATSRPLEYGLATLRMESGVI
                     AHVEGSWAHPRPFSYKFEISGSEGMIECDSAKTKPLTLLTLAQPSSDCSKNEKAREGG
                     PEAGLGVATSESPLRERDPYLREIQDFLAYVRGERLPRVLPEDAVAALEVSLAATRSA
                     DLGEAVKVR"
     gene            13300..14274
                     /locus_tag="PLU15_06395"
     CDS             13300..14274
                     /locus_tag="PLU15_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO30588.1"
                     /translation="MSFAHMHAYSYARAVCSLPDAQLVGVADDDEARGKAAAEQYGID
                     YFNDIEQLLDSGVDAVIVCSENARHRDHVVAAARAGAHVLCEKPIAADVCDGAEMIEA
                     CRAAGVVLATAFPVRHVPAVARTKQLVEEGKIGKVLAVKATNHGRMPGGWFIEPDLSG
                     GGAVIDHTVHVADLLRWILNDEFKSVYAEIDTRFYDFDIDDCGTLCLEMENGVFATLD
                     PSWSRPRTYPTWGDVTMEIIGTDGVISLNAFAQCLVEASDSQGAVVYRGWGDNSDLAL
                     VADFVSAAREGRRPAADGVDGLRALEVALAAYESSRLGEVVALDKLDC"
     gene            14354..15622
                     /locus_tag="PLU15_06400"
     CDS             14354..15622
                     /locus_tag="PLU15_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30589.1"
                     /translation="MTKNMSRYGALLLAVVLLVGLSCNVAAQKKVTIVHWQHHSPARL
                     ELVQTFAEEFMKQNPNVKIEIESVPESDYFQKLLPALAAGSGPDTFQIPTGQVPAYAA
                     SGVIQQIDPRVATARQIENDFIAPAIKRLKYDGKYYGFPVDTQTIVLFYNPKLFKEAG
                     LDPARPPQTWDELIKYAQAIHKRDVKGVTTQMGVATGGYGPVLVSLMIQNGAPLWDNK
                     TQLPDFNSPQAIEALKFATDMVTTYKVEDPQFGQRYNAFRQDKLGMVFGHPAMIGSFR
                     TTAPNLDFLVAEVPAPKAGGSRSTLLTSWAYVVSSKAESEICSKWVAYLTSADAQKRW
                     TLTTGELPSRRALLDLPEYKSDKLLAPCMESMKKSVPAPWTTRSLDDELVRKAYQMVT
                     LKGESPAAALKWLTQEGIKAEKEQQELAKP"
     gene            15836..16768
                     /locus_tag="PLU15_06405"
     CDS             15836..16768
                     /locus_tag="PLU15_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30590.1"
                     /translation="MSAEATTIPPRNGNERKYKWYRFQKASAPYIFILPGVVFYLMVT
                     IVPMITGFWMSFHNWNIIRPTQPWVGLANYRKLFADPLFWTAMKNTLTYSIGVVPLQM
                     ILALLVALLLNTGIKGKKFFRLLYYLPVITPLSIAAVIWTWIYHPNLGIFNQIVKFIG
                     LAPQDWLGDPAWAMFSVILVAIWSGIGYRMVVYLAALQGIPRSYYEAAEIDGAGNWDA
                     FRYITLPLLKPTTLYILVTSVISSFQVFGLVNVLTGGGPLDATQVVVSYIFKRAFGDL
                     QFGLAAAMSFVLFGIILVITLIQWKFLGKEVTYE"
     gene            16765..17667
                     /locus_tag="PLU15_06410"
     CDS             16765..17667
                     /locus_tag="PLU15_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30591.1"
                     /translation="MSANVNSSTLDSALRNQKNQGPAKASKWGKIIGKVLAYIVVTLG
                     AIMMALPFVWLILSSFKTLEEISLFPPTFFPKNPTLSNFREVFERIPFARYYMNSIIT
                     SVIPTVVTVITCSMAGFGFAKYRFAGRDVLFWVILATMMVPFPATIVPLYVMMAKAGL
                     VNTYAGLIIVGLSSASGIFMMRQFMMTIPDELLDAARIDGASEFRIFNQIVIPLVKPA
                     LATQTLFTFTGHWGAYIWPLICVNSDKLRTLPLAIPYFSGQYFSYQNLINAASLMAIA
                     PVLILFLFTQRYFVEGITITGMKG"
     gene            17809..20622
                     /locus_tag="PLU15_06415"
     CDS             17809..20622
                     /locus_tag="PLU15_06415"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOO30592.1"
                     /translation="MIRVFAGRVRPQGRRPVRSATVVAALVLCLMIMAFGPADHGANA
                     GVLTWLCPGSCSAGENVAARNVILFIGDGMGPGQIEAARDVSPFMLSMDFMPVMTRIT
                     TNSWGDNLTDSAASSTALATGHKTVNRSLGVLPDGTPVPNIVEAAKESGRYTGLVSTC
                     SITDATPAGFSVHVADRGSGREIAAAMLETGLDLIVGGGASYFLPTLFEKTPPPIEVA
                     RSKGYTVVQNKEDFLNASSVPLLGLFSMTHMDYDVQRNPDEQPSLAEMTAKAIELLSS
                     TEGEGFFLMIEGGRIDHACHNNDAEMLISEMLAFDKAVSVGLEYASGRDDTLVIVTAD
                     HETGGVDVSSGEIQFSTDGHTTEPVFLFAQGKCSELFASASDNTDVAKLMAMAMQVNL
                     GEVYTVEEGLAGATEKEAPIEEDGTDGAVSCVEEQSRASVGLRERPRLEVKPAHRVNV
                     MTYNIHSGFGADLVLDLDRIADLISEQKVDIAGMCEVDCKTGRSGGVDQAAYIASRLG
                     YEHVYGRTIYYGGGQFGNALISRYPIRSWCNHQLPNPAGNEPRAVLEAQVDVDGTILN
                     VFVTHLDVKDETGRRAQVGALLDIASKAVGPSVIMGDFNASPYADEISLALHEFNDTM
                     PTYRVLVNSDELVKEGLFARDYLKGGYTYDAYSPARRIDYIFTSFDIGIVDEPGAARV
                     PQTLASDHLPYIAAIEVPSADWSGQNDMTDAARTPLVTVFTGRANEEWYEDMRWDWQD
                     HINAIFDVVEEAGYECVEVAEDNIEVLWQVAQVRPTTLILSNARRMSDAQLQAVRDFV
                     AAGGRVLATGQTSLKSVDEKASGAHGFYLADLFGVSFVGWQGVAPLHSIIMPTSDGGV
                     LESPIEVADSRAIIVRRLPGTCELGTWADDDGSPTHADEYNSAVVLSGRVIYVGCDIF
                     APEMLSDDVRSFVADALDALFQL"
     gene            20732..23098
                     /locus_tag="PLU15_06420"
     CDS             20732..23098
                     /locus_tag="PLU15_06420"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOO30593.1"
                     /translation="MPQMQNSRIRIEVDGKTGEVRSIFNIVSRDELVKWPEAVHGAPL
                     RIVCRDLQGHRRVFAPGPASIATATKFNGVDKLALNVEHRTVVASGEAITPCIGSGGE
                     EDDASGLLGCGRGEDEIEIACSWSVELDEENPEISKWALSVDNRSQDYEVIEVLFPYV
                     RGIQVGVAPEDSILIFPHHAGEKIENPASRLASDRYMKFWRAESRIEPDGTYSREINY
                     CGLASMMWMDCYDRNGSGGLYMASYDSDFILTGIRSETGGPDDPWCGFGFRKYVPIGP
                     GEVWSSHPYVLGVHSGDWHWASEQYRSWAEEHLTVTGVPDDLARESAICPRYDFKNGQ
                     TVRNRFVDIPEMYGTAHREHINHFLISGWNRQGFDTDYPEFIPDMELGSSWQLREGCR
                     HVRSEGGFTTFYINVRLFDTESKFFDSLGRAWAAKGLSGEITHEMYGPRTFAVLCPGC
                     GDWQDWAVDTASWMVDAFGARGIYLDQLGSATPLPCYDSGHEHVKVGNRGERPHHGLY
                     NHGYVKMIERIKERCRLIDPSSFLMIENCGDVYSQYLYANLTWNGEEYDEFFNMYKYT
                     FPEFIQVNMVNPRRIDDRDLRYAWFFRDLARAFVLGSVFWMELGDRFGPGNEELREEA
                     LEALRLRQQAAPYIARGTYRDDLGLAFIGECDTAPDPAPRIWWDDEDGSEGLEGSFGH
                     SCDWTCLPFSDLISVTASRWTLAGGGVLILMSNPQRLKGKRVWVEDGCGGGAISGSRR
                     HAIQYELGGVRSDLEVVIDDRGGVTLEVPESRLSFIAVLPKEMVIGGR"
     gene            23102..23935
                     /locus_tag="PLU15_06425"
     CDS             23102..23935
                     /locus_tag="PLU15_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO30594.1"
                     /translation="MNAGERREGSGPVDMHIHTNASDGQPTADEVLKQAKAKGLSMVA
                     ITDHYDPFAAKYGAQSGNVFDDKSLAEQLKWRDGLAGEDDIEVFIGIERGPIPVGTAS
                     LPMQPDFVIGSVHYVTSKIPVERGRLYDERYWRAYMDEVLRVASDPEVDVIGHMAGYL
                     PMGQMLLPGSTFEERRQMEREIDARFFSREWYEDVFRAALANKTAIELHCATCTPRID
                     MVELGLKMGIKFSVGSDAHNVESVGKVAWAYDTLQSVGGGRHNLWCPLPQTRVGARPV
                     R"
     gene            23948..24676
                     /locus_tag="PLU15_06430"
     CDS             23948..24676
                     /locus_tag="PLU15_06430"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO30595.1"
                     /translation="MFEYFDVVRSLLDEVERAEAENMEEAATLIAEAIADGRVLHYFA
                     AGHSHMLGEELFYRAGGLVPVNAIVEPALMVTFGAGKSSHMERLTGLARIILDDAGVG
                     SGDVMIVASNSGVNPVPVEMAAEAKSDGVKVIGLTSVEGSRTTPIRNSVGKRLYEIAD
                     IVIDTHVPWGDAAIEVEGVSQKTGPVSTVIGAAILNTVVVRVIERLVERGIDPPIFMS
                     SNVPGGLEHNEVLLKQYRSKIKSF"
     gene            24698..25507
                     /locus_tag="PLU15_06435"
     CDS             24698..25507
                     /locus_tag="PLU15_06435"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOO30596.1"
                     /translation="MDAGYWAAKPFIDQRLGGLIGMLGLGAKYLDELRKLIDRIEKTQ
                     SESIAEAADAIVDSVSQGGAVHVLDTGHMLMNELFGRAGGLMMPRPVNIKMEVDNPGP
                     ARPGKTKEMVYLDEIPGLPEFILDRSDIYPGDVLIIGSVSGKNALPVGLALLARERGI
                     KVIALTSVAYSSSLEAQHPSGKHLFEAADIVLDNCGVIGDAILEVEGLDTKAIPTSGI
                     AAACLMWTLEAEIIDRMLKRGLTPQVWKSNHLPGAGEFNRKALAETERVGY"
     gene            complement(25543..25767)
                     /locus_tag="PLU15_06440"
     CDS             complement(25543..25767)
                     /locus_tag="PLU15_06440"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HOO30597.1"
                     /translation="MAQRIGAILFQIGGMMTVIGFILVRHPKAFSWFGHLPGDFMTET
                     VIAPIASMLVISLGLSALSWIFGALLRLIR"
BASE COUNT         5748 a         6533 c         7590 g         5963 t
ORIGIN      
        1 aggaggcgca tggcgcgctt tgactgatgg acggatgaat gtgcgtccgc gggcgctgat
       61 tcaggcgagg ctcagggctt atcggatttc gcccgcgggg ttttgggtgt atgttctctc
      121 cgtggggcta tatcagctca tgggatttgc cttcgtcgtt gccaccacat ccaggccgat
      181 atcggtgttg taatgccgat tgcccatttt gaacccggcg aaatgttcgc ttcggttgcg
      241 ggtttgccat cgctctattc ggcctgtgga tcggaggacg attcacggcg atcaatcttc
      301 agtgtacagc ctcttcgtga tcgcggcctc gctcgttctg tacaacaggg gtctttccgc
      361 ccgaatcaac ctggcaatgg acctcgtgca attcgggcag tatccgatgc gcttctgccc
      421 cgacgctatg cgcatcatct ttacctgggt aatccccctt gcagtggttg ccaatgctcc
      481 agcctgcctc gtagaagcgg ggttggcaga gatgccgtgg cactttattc caggattcat
      541 cagatgcgga cctctggccc tccccggttc caatgcaatc ttcagatagg gaataaggcg
      601 gtttttgaga tattggtaaa caactgctcc cctgtatcct cccacggtgg caatagaggg
      661 aattgccaac cgaacttgca catccatata atgggagggc acgctgcctg tccgcggcaa
      721 gagggtagag acgaaacccg atgttcgcgg ggagccgggg cggagcggga tcgcgagcgg
      781 cactttctta cgaggcaggg ggaatggtgt gggcagcccc tgaactgggt gtgctgatgg
      841 tgtcggattc acgtgcgcgt gcctcggact gtggctgtgg tatttcatct gatctgcctt
      901 gcttttctga aggcatgttc tttttgatca tgccgacagc catcaggaca ccgccaacga
      961 ttagattgag gtagtaggtg aatgcgcgcc atactgtgac gaagaggcct atgaggccat
     1021 gcggaaccac actgctaaat agcaggctga agccgagctc tgcagcgcca ctcgatccgg
     1081 gtgtgggagc ataggcggct gcaaggtaga aaatcagagt gatccccata atgtgcgtga
     1141 acttgggtgt tatgcccaga cccaccagga ttactgctgg aactatggtt atcgtgatcc
     1201 accacgcgta actgagccac atcactttcc aaagcgctga ctttttgttc tccatgaatt
     1261 cttgcaatgc atttctgaac tccctgagct cgtgatcgat gcgatcgagc actgcatcta
     1321 aagtctcggg cttgaagaac ttcagggtaa acttgttgcg tacaacaggt acaacgataa
     1381 cgttgatctt ttccgggtga aagatcaggt agagtgagaa cgcaaacacc acgaaataca
     1441 ggatgatgcc gattgcgagg agaacgtaca tagctttggg aatgctccag aagtcagcaa
     1501 aaccaaataa ccagatcgat gcggctatgc caagagtgaa gcgggcaagg gcgttgcata
     1561 tggtcttcac tgcgataact gccgtagatt gccctgcatt caagcctttt gcagttagaa
     1621 ggtacgcctg cataggctcg ccgccgctat cgaatggtgt tacgttagac acgaatgccc
     1681 ccatgatgga gatgcgcatt ccggtcagga tgtccaagtc tcccccaagc gatcgaatga
     1741 gagccctcat ccggagcgaa tcgaacagcc atgttactat cacgagtccg cacgcgatag
     1801 ctagcattgc cgggtctatc tgtgcaaatt ccttccatga cgttcgtccg ccggtaaact
     1861 ggagcacaac gtagagccct agcacgctga agaccacgcc aatgagcgcg ctccgtctga
     1921 gctgagcagg ggttctacgg cgggtttgct gttcctcact catggggttt cgccccctta
     1981 tagcgggttt ttgcaaaaac acaacacatt atatcatagc catggggcgc aagcacaaga
     2041 gagttcccga ttatatggaa attaaggcaa gcttttgtcc ctggctgaag ttagctggaa
     2101 ccgactgcgt actgagcata caattgatca atctacactg ccataggtgt ttatgcccgg
     2161 caagcaacat ctcgggcaag tctcgacagg ttcctgcgcg attccactta atgattgtct
     2221 ggatttcaag aacacatata gcagcatacg aggtcagggc tgtccacttc ttgcgcccct
     2281 attgtttcac tatgcgcgtg cctgcgtttc cgtgaatggc ctcaaccgcc ctgtcgagtt
     2341 gcgcgattat ggccgcctct cctccgcgct caacgaaacg gcgacacgct tcgaccttgg
     2401 gtcccatgct tcctgccttg aagtgacctt cctgttggta acgaatgagg tcatccaatt
     2461 ggactgtgtg caagtcgacc tgatggggcg ttttgtaatg cagggctaca tgggccacgt
     2521 cagttagtat gagaaggacg tcggcaccaa catccctagc aagacactcg ccgcctagat
     2581 ccttgtcgat aacggcctcc actcccttgt agagaccgcc cttttctata acgggaattc
     2641 cgcctccgcc gttagcaatt acgattgcgc ccgttcggac cagtgagagg atggcgtctt
     2701 gctccacgat tctgatggga tcaggcgatg ggactacacg tctccagccg cgtccggcat
     2761 cttcgatcca cttctctccg gtctccttca ttcgctgctc ggcatagtaa gcgctgtaga
     2821 atggccctac aggctttgtc ggatttgcga aagcgggatc attcggatcc accaccatct
     2881 gcgtgattac ggtcgctaca ggcctcggga tcgatcggcg cgccagcatc tttccgatgg
     2941 attgctggag catgtagccg atttgcccct gggattcggc gccgcataca tccaatggca
     3001 tgggagggac cctgtcgcgg cccgcttctt gttggatcag gagtgctccg acctgtggcc
     3061 cgttgccgtg ggttatcacc actctgtggc cttgggctat gagctcagct acttgttccg
     3121 cggtggaatc tacattctcc atctgctcat cgacagtccc gttctgcccc ggctgaagta
     3181 tcgcgttccc gcctagggcg atcacgatgg tcttggcatt gtccaactca aacactccag
     3241 tcttgcacat tgtccgccat gcttcggggc gctgggcgcc ttggcgtccg aatcatgcat
     3301 tgaagatcac cttgcccatt gagatcgtcg ttgttgccct ataagtgttg gcatcgagga
     3361 tagttatatc tgcatccatt ccccgctgga ggcggccttt tcgatcgtcc agccctagct
     3421 ggcgcgctgg tgtcgaggaa gccatggcga tggcgtcagg cagcggcacg ccgacgagac
     3481 gaaccatgtt gccgactgca acatccatgg ctagagtact accggcaagt tcgcctgatt
     3541 ccaggcgggc ctgaccctcc ttaacgatca catgctgggt tgcgagggtg tagtctccgt
     3601 cgggcagtcc tgctgcgcgc atcgagtcgg tgaccaaaac aacgtgcgca gcccctttca
     3661 tcccgaccac aatggaggcg gcgatcggat ggacgtgcac gccatcgatt atcagttcgc
     3721 atgtgagatc atcgatcgtc agcgctgcgc ctacaacccc tggctctctg tggtggaggc
     3781 cgcgcatgcc gttataggtg tgagtgacat gcctgattcc agctgccacg ccgcgttcca
     3841 tgtcggcata ggctgcatcg gaatgtcctg cagaaaccag tattccctgg gacgcgaggg
     3901 cgtggattat gtcgattgct cctggaatct ccggggctac cgtcatggcc atgatgtggc
     3961 cgcctcccgc atctaagagc ttctcgagga cagagatgtt gggggccatg attccttctt
     4021 cgggttgtgc gcccttacga atcgaattga gaaacggtcc ttcaacattt atcccaagga
     4081 tgcgcgcgcc ctgagtacag cgtgagccta gacggtcagc atctatcgcc tctgcggcgg
     4141 ctttgcatgc acctgtcatt tgctctagag gggaggacat gattgtcggc aagaagccaa
     4201 caactccatg ggaggcctca aactttgaca tgcagttgag cgaatcgaag ctcgagtcca
     4261 tggcgtctgc gcctccgccg ccatggacgt ggagattgat gaatccggga gctattattc
     4321 cgcccttagc gtctatcgtt gcatcgaccg tttctttgcc gagagaagcg gccgaaccga
     4381 cattgagtat cgtgctgtct tctatgagga cagctccgtt tgcaatcacg gatgtgggtg
     4441 ctattacagg tccgcctgtg attaaggttt tatgggccat tctttaaact tccttccaaa
     4501 agagttcgac gcaggcatta tcttatgccg gacgtctacc acccgaatgt tattctcctt
     4561 agtcgcgctt tcttccttca tggggacggt tatcgcatat catcgagtat tgccgctcag
     4621 actagtgcta agggaatgga ggactttctg ccatacaact gtggaagcac gggacgtaac
     4681 ctcaaaccga aatcgacaaa aagtcggctg agggacgctc acaagtggcc agcactgtag
     4741 tggttgatta cagctgtgag caaggtgccc ataatcagcg gctgattatg ggcggatcga
     4801 cacaattcgg ggattggagt gatcagggct tgagcaagtt gcgtgtaggc gtaataggaa
     4861 cgggcatggc attcgaacgg ctccactatc cggcctacga gaggctttca gatgtttacg
     4921 agatcggcgc cttgtgcgat caggatgctg gcaaattggc ggattgggga cagcggttaa
     4981 ggttgggcgc tgagagcttg tatactgatt ggcgcaagat ggtggccaga gatgaccttg
     5041 atgtgatcga cataatggtc cccatcgagc tcaattacgc tatcacagaa agggttgcgc
     5101 gggagctggc aggcaagcgg aaggcgatta tctgtgagaa acccttagcc ggcaccttca
     5161 aggaagcact ttcagcaaga gagctggcgc agcgatacag tgtactgatc atgatcgcag
     5221 aaaactacag gtacagtgag gaaatgaaca tcattcggga cctcgttgtg agcaggcgag
     5281 tgggagatcc ggtgtatttc atatacaacc gaaccgtgga tttcccctcg gacatgtgga
     5341 aagatgattt tgccgcaagg gagtggcgac agtatcccga gtttcctggc ggcgccattg
     5401 ccgacatggc agtgcacgac ctggccgcca tgcgccacat ttttggcgca atcgatcggc
     5461 tgcaggcttt cggcaggccg caagatgcgg agttctctcc atacagtgta atagtggtaa
     5521 acatgctctt tgcaagtggc atgatcggcc aattcagctt cttttgctcc ggcaaggaga
     5581 tgcagcgccc tcttgtgggg ttcaggatct tcggcaccga aggaatgatc tatctggagg
     5641 accggaactc aggagtgata aatgtcgctt acaacgacag gcgttcggaa cagatccctt
     5701 atgatcccca gaaagcctat tacaatgagc tgctcaacct cgccaacgcc cttagaggaa
     5761 aggagccggt aagtgtaccg ccggagatgg aatatggaga cctgaaaacg gtcagagata
     5821 tactaaagtc cattcaggag cagaagatcg tcactgtgga caagactgcg gattatcttc
     5881 ccgactatgg ccagccggga cgcgaagggc tatggctaca gtaggctagc ctcaagacgc
     5941 gtcacatgga acgggcgggg ctggcggcat agctcggagc caaggagtca acgtcatttt
     6001 gacaagaaat ctggctgtat ggcctccata tagattccgc cggcgggccg ggcttgtgcc
     6061 cggcttcttc attatttagt gcgccataag gggataagaa gttgtggcgg ttgtttcttc
     6121 ggcctaacgt tcagttttgt tgctaggagc ggatttgtgc gatccggcat ctgcgacaaa
     6181 aggctctagg tctgaacgga ggattctcca atcccgtcct cgcttgatgc catggagacg
     6241 gccgtctcgc atcattttct gtatagttgt ggtcgaaacg gctagtactt ccgcggcttt
     6301 gtctatcgtt accgtcctag gtttggttga aacggctttc cactttttct cggtcatgat
     6361 gacacctcca ctacttataa taacataaag ccactcgaag gaggttatgg ccaccagaat
     6421 gggaacaaaa tgaataccgc tcgacaattc tattatggta ggatgttctt gaagtagcca
     6481 ttcattggca gttccaatct ggcgcgagga ggcacaaaat accatgttca aggccaggta
     6541 tctaccttta ctgcttttag cgctcatatt cataatgtcg atctttgtgt acagggtgct
     6601 ccccgataga gtcccaatgc acatgaactg gaagggcgag attgaccgtt acgggtcgaa
     6661 ggcggaaggg gcctttttct ttcccgtcgt tatactcatt gttcatctga taatgtcgtt
     6721 ggtcttcagg gcctacataa acagtatcga tgcggacgcc cgtagcgttg ccgcggatag
     6781 acttggcatc ttcaaaagcc ttttgattct gctttttgcg gccatgtatg cagacttcgt
     6841 aatggtcgcc ttgggcaggc cgtccagtat gtacgagttt cttggcatcc ctgttgcact
     6901 gcttttctat tacacaggcg ttctgatgaa ggatacgccc agaaatggga taatgggggt
     6961 aaggacgaag tggtcgatgg cggatgatga agtatgggtg aagactcacc acatggcgtc
     7021 gacggtgtac cgtgtgagcg ctctgttccc actgctctcc ctggcatggc cgaacctctt
     7081 ttggttgtgg cttgcggttc cattgatcct ggctcccacc gtgatgatat ttgcatcgag
     7141 gcgttactat atggagctgg tcggaaggaa atgatcgaac agttgaaaaa tggcggcgga
     7201 atcgcgggag ccttgcgggc tgcagttgac tcatgccgga tttgacctga aaaccaggta
     7261 attgcgagaa tcttcatgag aagaggacat cgggttgcag ggattgcgat tgttattgcc
     7321 atgtccgcta tattaactat tttggccgat ggcgccatac agctgaccat aaacgggcgg
     7381 gccccttgag gccgataatg tcccagagtt gacaatggca aggtggtggt gctgttgcag
     7441 aggcgcttgg ggatttgcgg gcccaaaaca tcgtcgcttg agcggcatat cagacttttg
     7501 gaggaagcga tatcgccatc tactcccagg gaggccgtgg agaagtgggc cgagacgcgc
     7561 aattgggcgc tccagtatgc ggtgatctat cctgaattgc gagaggtcgc aagatgtttg
     7621 gtggtattcg actcttcaac atctacagga cctaccgtgg ccagaatatc tgttgaaaga
     7681 gacaagaatc gagccaggct ctcgcggcat atgtcggagg agaggtggca tgtggtaaag
     7741 acttccgtaa agccggagtc agactgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnntgg tcaaggactt gattgaggag aagtaagatc ttcattttcg acgattagcc
     7921 atgaaatcag caggaagcag gcatcatctc gctgcctgct tcctgcggga aatactcttg
     7981 cttgtcatca atgggaatta ccgaccaaaa gctcctcgcc cagctgagct ttgaaggcct
     8041 caatatagat tccaacagcc actatagaca tgccgccttc actggaaggg tcaggcgccc
     8101 cgctgatttc tattcatcca caaccaaagc ctcgtagagc acgcgccctg ttgcgtttgc
     8161 cggctgtctg agccccatca gcctcgacat agttggcgct gcgtcgatga tctgcactgg
     8221 cgaatcgatg acgactccct gcttcacacc ggctccgctt atgagcatga atacatgccg
     8281 ggaagattct tcgcccgagt catgttgccc cccaaggccg attagttttc cggaagtgtg
     8341 gaaatggttg ggcggctcag gccaaatcat gagagatccg agggacggat gggctccttc
     8401 tttcttcagg tcatctgccg tgaaatagcc gcctacatac ggctttgaag ccaatgtatc
     8461 tataagagcc ttgtactggg cgtcagagat cgcgtttctg aaatagaact gtaccgaaag
     8521 gccggcgctt accagcacta tgtccgtgtt cttgctggca cgctcgttct tgcccaagaa
     8581 ctcgcacttg tagccgagac tttccacggt ctttatgaga tcttggaggc tcgatggtcc
     8641 ccagtaggga gtcatgccgt ggtctgctgt gaggatgatc gctgtttcct cgaatattcc
     8701 tagttttttg agggcgtcga tcaggttccc cagcccctca tccatgtcga tcatgagttt
     8761 ggtcatcttt gccttccacg aatcataatc tcgtgccact gaaaacccgt agccaggcga
     8821 agcgttgtgc cccgcagcgt cgaggtcatc ggcgtagatc gccatgaagg tgggcctttc
     8881 ggcgggggtt atcatctttc cattcatccc gaacacaggt ttcaaattga gaatcttttc
     8941 cgcttctgcc acgcgcttca accatcccgc gccaggctgg atatacagat ggtagggatc
     9001 gtcagtatag gttccccgct cctgcaacat gaattgctgc acagatgcgc acggtaatcc
     9061 gctcttctcc acgacctcgg caatggtctc agcgtcattg tgacggccgg tctccttaac
     9121 gaggttttcc acagggtcgt agtaacggta tgtattcccg tgcacagcgg gccatgtgcc
     9181 agtgactatg ctcgtttgca tggggttggt aatgccggga ataccgtttc gggcattggt
     9241 gaagagtgct cctcgcgaag caagataatt caggttggga gtgcccggtt ggccgggtga
     9301 attagcccat tcataatacc tgtaagcaca ggcatcccag ttgatataga tgacgtactt
     9361 ggccagagcg ttctgaccaa gggcatcttg cccggctgcg ctgtctgcac cgggctttcc
     9421 gctatttgcc caggttgttg agaccagtgt caatgccaaa acaacaatga taatgctgca
     9481 taaaactgtg ccgattgttg ccatcccctt tttcatgcca tccggcgctc atcgacgtat
     9541 ccttgtggaa acgtggggga gagcgccttc cctccttact ctgctagaat tatgctctgc
     9601 gcacagaggg tgcgccagga atgctcactg tgccccggac ggctttggtc cggcggctac
     9661 tggtcatccg acatcttcga atacgcgact ccagtagaat ttgtcgaaca cgagcccggc
     9721 gaggcccagt gcaattccca tatcgccaag ctgtgaaggg actaaccgca cgttcttgcg
     9781 cggggtcaat accatgcgat ctcgaactac cgtttccacc tgtgatacaa actgaggttc
     9841 ttggcctata tctcccccaa gcaccaccag ttccggatcg agcaggttca tcagattcac
     9901 aatgccgatt gcggcgcaat ccacgataga atcgctttcg ttgtttcgcc tcacctcgtc
     9961 aatggctggg tatgtaagcg tcaccagaca gcctctgttc ccacaagagc attgcttccc
    10021 atggatgtcc acggtcatgt gcccaatctc gccagcctgc attcgcgcgc cagcgtaagg
    10081 tttgcctccc aagatcagcc ctgcccccag cccgccctcg caaaccatga cgtatacgaa
    10141 atcgtcagcg cttcgggcga ggccatacga gcgttctccc cacgctgcgg cacgcacatc
    10201 cctttccaca taagaaggca atgagaactc ttgctgcaag cgggccgcta gaggtagctc
    10261 tttcagacga aggcgggtcg acttgataac ggtgccggtc gttgtgtcaa ctatgccagg
    10321 aatggacacg cctattcctg ccgcctgtcc gccagaccgt ggcagcctgg caagggtttt
    10381 cgctactatc tgaaagatct ggtggtaggt gtcttccgga cttgtcaggt cgagcttgtc
    10441 ccttgcagtc cagactgggt tgcccgctag atctatcacg ccgtagagca cgcgggatac
    10501 ctgaacatcg attatggcgg cacagcttgc tgaggcgtcg aggtcgagca taatgggacg
    10561 gcgtccgccg ctgctggcag cagtgccctt ttgctctcgc acaatgccgg attccaccag
    10621 ttctgctact atgctagaca ccgttgatgg gctgagcatg gttagcctgg caatatcggc
    10681 acgcgacaga gtcccatatt tcctaatgca atcgatgact acatctctat tgagcttctt
    10741 ggcgagcgcg gaatcagcag tgcgtttgag tttcatatgt gcttacctct cagcgtgtag
    10801 tcaggttgcg ctatcgaact ggcacaaacg gcgcgaatga cgattgcgcc agtggcgtga
    10861 tttttagagt ggcgaataaa ttcgccatgc aactaaacgg cttcgaatca aatgccttta
    10921 cattatcata tggtacgttt agtgacaatg caagattaag atgacgccag agacagatct
    10981 ggtctgaact ggccatttaa cgtgaacttg acaatcgttg actctgctga ggccaggtgg
    11041 tatattaata gtacattggc atggcagaag cgccagtaca ttcgactctc gcagaaacta
    11101 tcggattacg ctggagacgt acgaccctgc attcccgcga tgggaatcgg ccgggaaaac
    11161 tcccggtgac tctacgacat ccatatgggt gttacaagat ctaaaggagg gtgcagtcat
    11221 gtctaacaag aagacaggaa cgattgtcgc aaccgccgct ttggccgcga tcatgctgct
    11281 ggcaagcata ggcgtttcgg cgtcagcggc cgacatcttc gccggatgga gcgagcccat
    11341 gctctgggag cattccggtg gcgacacaga gaggatgcca tatttggcgg gcaacgatct
    11401 ttactttgca aaggattacg acatctactg gtcgaccttt gacgaaacca caggcaagtg
    11461 gtccgagccg gagccggtcc cgggaccgat caacaccgca gccaacgaag cctgtccctg
    11521 cgtagttgga aacggaaagg tcctctattt ttcgcgttat gaccccatca cggattacga
    11581 tttctatcgt tcagaatggg acgaggaaaa agccgaatgg ggcgagcccg agcttatcga
    11641 agaactgagc accgacggac aggactgggc gatacacgta aacgaagatg agacgatagc
    11701 gtatgtgata tcgaagatac cttacgacga ggcaaccgtt gtgggcggcc gcgacatctg
    11761 gaaatccgtc aaagtcgacg gtgtgtggca gaccccggtc aaccttggag agccgctcaa
    11821 cagcaacggt gaggaatgga gcatcttcgt cgattacatg ggccgttttt ggatagatgg
    11881 caaccgcgcg gactccctcg gcaacttcga tatatatgta gccgagaacg agaaagcgac
    11941 tccgatcaat cttggcgcgc caatcaacac tgacgtggcg gagcgcgaac ccacaataaa
    12001 cgagaaattc ttcttcttct ctgccaacaa gagagaggga ggccatggtg gctgggatct
    12061 ttggtacatg gtgaggctgc cataggacct tcgtccccta agatcgcagc gatcacaatg
    12121 tcaattggtt aggctcgacg tgaaggcagc ttgaggttgg catcgtagcg cgaaggcggc
    12181 ggccttggaa atcgacctaa tacagacatg gattgtcaag gagggctatt gggtgcatac
    12241 cgtggctatc tggggagccg gcaccatggg ccgggtccat gcaaagcgtt atgccatgac
    12301 tggcgaagct caactgaaat acataataga caacgatctt gagaaagcga cccggcttgc
    12361 acaacctttt gatgccgttg caacggtcga tccaggcccc gctgttcgcg atccgcaagt
    12421 ggacgtgatc gatgtctgcc ttcccactta tcttcacaga gaatcagtgg agcttgccgc
    12481 aggccatggc aagcacgtat tctgcgagaa acctatagca cttacactag atgacgcaaa
    12541 tgccatgatt tcaacgtgca agtccaatgg cgtgaaactc gggatagcgc atgtagtccg
    12601 ctatttcccg gaatatgaaa tggcgcgcga catgatagcc tctggcaggc tggggcgcgt
    12661 gggcgttgtg cggctttccc gcacaggtcc gttcccgggt ggatggaacg attggtacgc
    12721 cgacccccag cttagcaagg ggctttttgt agatctatcg atacatgatt tcgactttct
    12781 gaactggtgt tttggccctg tggaacgaat ctgtgcgcgt aaggtcgcaa cgagtaggcc
    12841 cctggagtat ggccttgcaa ctctacggat ggaatcgggc gtcatcgctc acgtagaggg
    12901 cagttgggca catccaaggc cgttttccta caagttcgag atatctggaa gcgaaggcat
    12961 gatagagtgc gacagcgcaa agaccaagcc cctaacgttg ctcactcttg cacagccttc
    13021 ctccgattgc tcaaagaatg aaaaggcgag agagggaggc cccgaggcgg gtttgggagt
    13081 tgcaacttcc gagagtcccc tgagggagag ggacccgtat cttcgagaaa ttcaggattt
    13141 cctggcgtac gttcgaggcg agcggttgcc ccgggttctt cctgaggacg ctgtggcggc
    13201 gttagaagtc agcctggcgg cgacccgctc ggcggatctg ggagaggcgg tgaaggtaag
    13261 gtgacggatg gtgcggagaa gaagctccgc gttggagtaa tgagctttgc gcacatgcat
    13321 gcctacagct atgcccgggc tgtctgctca ttgcccgatg cccagctggt tggggttgcg
    13381 gacgatgacg aggcgagagg caaagcagca gccgagcaat acggcattga ctatttcaac
    13441 gacattgagc agctgttgga ttcgggggtt gacgctgtca tcgtctgttc cgaaaatgct
    13501 cgccacagag accacgtggt tgctgcggcg agagcaggcg cgcatgtact gtgtgaaaag
    13561 cctattgctg ccgatgtctg cgacggggcc gagatgattg aggcctgtag agccgcaggg
    13621 gttgtgctgg ccactgcctt tcccgtccgt cacgtgcctg cggttgcgcg aacgaaacag
    13681 cttgtggagg aggggaagat cggtaaggtg ttggccgtca aggccacaaa ccacgggcgc
    13741 atgccgggag ggtggttcat cgagcctgac ctttcgggcg ggggcgctgt gatcgaccat
    13801 acggttcacg ttgcggatct gcttcggtgg atcctcaatg acgagttcaa gtcggtttat
    13861 gcggagatag acaccaggtt ctacgatttt gacatcgatg attgcggcac actctgtcta
    13921 gagatggaaa atggcgtgtt tgctactttg gacccgagct ggtcgagacc gcgaacctat
    13981 ccaacctggg gcgatgttac gatggagatc atcgggactg acggggttat ctcgctgaat
    14041 gcattcgccc aatgcttggt cgaggccagc gattctcaag gagccgttgt atacaggggg
    14101 tggggcgaca attccgatct tgccctcgtt gcggacttcg tctccgcggc acgcgaggga
    14161 cgtaggcccg ctgccgacgg cgtggacggc ctcagggcgt tggaagttgc tctggcagca
    14221 tacgagtctt ccaggctcgg ggaggttgtc gcactcgata agttggattg ctagaccgtg
    14281 atttgggtat cagtcaattg aattatgcgg ccgcgtctga ggcgcggtca gcaaatccta
    14341 ggaggcaaga accatgacca aaaacatgag caggtacggt gcgttgttgc tcgcagtcgt
    14401 gcttctcgta ggactctcct gcaacgtagc ggcgcagaag aaggtcacga tcgtgcactg
    14461 gcagcatcac tctcctgcgc ggctcgaact ggtccagacc ttcgcagagg agttcatgaa
    14521 gcagaaccct aacgtcaaga tagagatcga atcggtgcct gaaagcgact acttccagaa
    14581 gcttcttccg gcgcttgccg caggatcagg tcccgacacg ttccagattc ccacaggcca
    14641 ggtgccagcg tatgccgcct caggcgtcat tcagcagatt gacccgagag tggccaccgc
    14701 ccggcagatt gagaatgatt ttatcgcgcc ggcaatcaag aggcttaagt atgatggaaa
    14761 atactacggt tttcccgttg acacgcagac gatagtcttg ttctacaacc cgaagctctt
    14821 caaagaagct gggcttgacc ccgcaagacc tccgcagaca tgggacgagt tgataaagta
    14881 tgcgcaggca attcacaagc gggatgtcaa gggcgttaca acgcaaatgg gcgttgctac
    14941 aggcggatat ggccccgtgt tggtctctct catgattcaa aatggagctc cgctttggga
    15001 taacaagacg cagctccccg atttcaactc tccacaagcc atagaagcgc tgaagtttgc
    15061 gacggacatg gtgactacgt acaaagttga ggatccccag tttggccaga gatacaatgc
    15121 gttccgacag gacaagttgg gaatggtgtt cggacaccca gccatgatag gcagtttcag
    15181 gaccacggcg cccaatcttg acttcctggt ggctgaggtc cctgcaccga aggctggcgg
    15241 atcccgctcc acgctgctca cttcgtgggc ttatgtggtg tcaagcaagg cggagtcgga
    15301 aatctgctcc aagtgggtag catacctgac ttcggcagat gcgcagaaac gttggacgtt
    15361 gaccactggc gagctgcctt cgaggagagc actgctcgac ctgccggaat acaagagcga
    15421 caagctgttg gctccttgta tggaatcgat gaagaaatca gtgcccgcgc catggactac
    15481 caggagtctc gatgatgagc tggtaaggaa ggcctatcag atggtgacac tgaagggcga
    15541 gagccctgca gctgccctga agtggctaac gcaagagggc ataaaggctg aaaaggagca
    15601 acaggaactg gcaaagccat agtaagctgc ggaccgggat gtattgcaca acttcatctc
    15661 tatgtgggac tgtccccacg cgggtgagag cgcacatccg gttgtgattc tgtagaggtt
    15721 gtggcacatg gaattcgcgc agatgtgcgg ggccgggtgt tggcatcctg gtcccgcaca
    15781 tctcgcatcc agatgtccgg tcccagctcg gcttggataa ggaggttgat gcgccgtgag
    15841 cgcagaggcg acaacaattc caccacgaaa cggcaatgag cgcaagtaca agtggtatcg
    15901 ctttcaaaag gccagcgccc cgtatatctt catactgcct ggggtagtgt tttacctcat
    15961 ggtcacgatt gtgccgatga taacggggtt ctggatgagt ttccacaatt ggaacattat
    16021 ccgtccgaca cagccttggg tgggtcttgc caattaccgc aaactctttg cagacccgct
    16081 gttttggacg gccatgaaga acacgcttac atattcgata ggcgtggttc ccttgcagat
    16141 gatcctggcg ctgcttgttg cgctcctgtt aaacaccggg attaagggga aaaagttttt
    16201 caggcttctg tactatctgc ctgtgattac tccgctatcg attgcggcgg ttatctggac
    16261 ttggatatac catcccaatc tcggcatatt caaccagata gtcaagttta tcgggctggc
    16321 gccccaggat tggttgggcg atccggcctg ggcgatgttc tcggtgattc tcgtcgccat
    16381 atggtcggga atcgggtatc gcatggttgt gtacctcgcg gccttgcagg gcatacctcg
    16441 gagttactac gaggcagcag agatagacgg ggcagggaat tgggacgctt tcagatacat
    16501 cacgcttccg ttgctcaagc ccactaccct gtatatcttg gtcacaagcg taatcagttc
    16561 gttccaggtg ttcggacttg tgaacgtgct gaccggaggc ggcccgcttg atgcgacgca
    16621 agttgttgtg tcgtacatat tcaagagggc ttttggtgac cttcaattcg gacttgcggc
    16681 ggcgatgtcg tttgttctgt tcggaataat cctcgtaatc acgctgatac agtggaagtt
    16741 tcttggaaag gaggtcacgt acgaatgagc gcaaatgtca acagcagcac cctcgattcc
    16801 gccttgcgta accagaagaa tcaaggcccg gcaaaggcat cgaagtgggg gaagattatc
    16861 ggaaaagtgc tcgcgtatat cgtcgtgacc ttgggcgcca tcatgatggc tctgcccttt
    16921 gtgtggctca tattgtcgtc ctttaagact ttggaggaga tatcgctctt tcctccgacg
    16981 ttctttccga agaaccctac tttgtcgaat ttccgagagg ttttcgagag gataccgttt
    17041 gcccgttact acatgaacag catcatcacg tcggtgatac ccacggttgt cacggtgata
    17101 acctgttcca tggcgggctt cgggttcgcg aagtacaggt ttgccggaag ggacgtgttg
    17161 ttctgggtca tactcgctac catgatggtt ccttttccag ccacgatagt gccgctttac
    17221 gtgatgatgg ccaaagccgg gctggtaaac acatacgcgg gtctgatcat agtgggtctt
    17281 tcgagtgcgt ccgggatatt catgatgaga cagttcatga tgacaatacc tgacgagctt
    17341 ctcgatgctg cgcgcatcga cggcgccagc gagtttcgaa tcttcaacca gatcgtgatt
    17401 ccgctagtaa agcctgcttt ggctactcag acccttttca cgtttacggg gcattggggg
    17461 gcctatatat ggccgctcat atgcgtgaac tcggataaac tgcgtacgtt gccgctggcg
    17521 atcccctatt tttcggggca gtacttcagt tatcagaact tgatcaatgc ggcgtcactc
    17581 atggcaattg cgcctgtgct gatccttttc ctgttcacgc aacgctactt cgtggagggc
    17641 ataacgataa caggaatgaa gggctagaag gcgagtttca ggttatgaga aagcagggcg
    17701 cccgggctgt ggcgtccggt acgttcttcc agggccgact cgcaatggcc ccggttggcg
    17761 ccttgcccga acatcgcatg acgctattat gatgaggggg tattactaat gatccgggtt
    17821 tttgcaggta gagtgcgccc gcaagggcgg cgacctgtaa ggtcggcaac ggtcgtggct
    17881 gcgctggtcc tctgcctgat gataatggca ttcggacccg ctgaccacgg ggcgaatgcc
    17941 ggagtgctta catggctctg ccccggctca tgttctgccg gggagaacgt ggccgcacgc
    18001 aacgtcatat tgttcatcgg agacgggatg ggacctgggc agatcgaggc tgcccgcgat
    18061 gtgagtccgt tcatgttgtc tatggatttt atgcccgtga tgacaaggat aactacaaac
    18121 tcgtggggag acaaccttac tgactcagcc gcgagttcga ccgctcttgc caccgggcac
    18181 aagacggtca atcggtcgct cggcgtgctc cctgatggaa cgccagtgcc caacatagtg
    18241 gaagctgcca aggagagtgg gcgatacacc ggattggtca gcacctgttc gataacggac
    18301 gcaactcctg cggggttttc ggtccatgtt gccgatcgcg gaagcgggcg tgagatagct
    18361 gcggcaatgt tggagaccgg tctagatctg atagtcgggg gaggagcctc ttactttttg
    18421 ccgacgctct ttgaaaagac tcctccgccc atagaggtgg cgcgcagcaa gggatatacc
    18481 gtagtccaga acaaggagga tttcctcaac gcatcgtcgg ttccgctttt agggctgttt
    18541 tccatgaccc atatggatta cgacgttcag agaaatcctg atgagcagcc cagtcttgcc
    18601 gagatgactg ccaaggccat agaactcctt tcatcaacag aaggtgaagg gttcttcctc
    18661 atgatagagg gaggcaggat agaccatgcc tgccacaaca atgatgccga gatgctcatc
    18721 agcgagatgc tggctttcga caaggcggtc tcagttgggc tggagtatgc gtctggacgc
    18781 gacgacaccc tggtcatagt gacggccgac catgaaacag ggggagtcga tgtctccagc
    18841 ggagagatac agttcagcac cgatggacac accacggaac cagtatttct gtttgcgcag
    18901 ggcaagtgtt ctgaactgtt tgcatctgcg tctgacaata ctgatgtggc gaagctaatg
    18961 gccatggcca tgcaggtcaa cttgggcgag gtgtacacgg tggaagaggg actagccggg
    19021 gccaccgaaa aagaagcgcc catagaagaa gatgggactg acggtgccgt ctcttgcgtc
    19081 gaagaacaat cgcgtgcatc ggtcggcctt cgtgagcgcc caaggcttga agtgaaaccc
    19141 gcacaccgag tcaatgttat gacatacaac atacatagtg ggtttggagc agatctcgtg
    19201 ctcgacctcg atagaattgc tgacctcatt tcggagcaga aggttgatat cgcaggcatg
    19261 tgcgaggtcg actgcaaaac gggaagatcg ggcggggtag accaagccgc atacatcgcc
    19321 tcaaggctcg gatacgagca cgtgtacggc cgcaccatct attatggcgg cggacagttc
    19381 ggcaacgcgc taatctctcg ttaccctatc cgttcatggt gcaatcacca gcttcctaac
    19441 ccggcaggga acgagcctag ggcggtcctt gaagctcagg tcgatgtgga cggaacaatc
    19501 ctcaacgttt tcgtcacgca cctggatgtg aaggatgaga cgggccgtcg tgcgcaggtt
    19561 ggcgccttgc ttgacatcgc gtccaaagcg gtggggccga gcgtgataat gggagatttc
    19621 aacgcgtctc cctacgcaga tgaaatatct ttggcgttgc acgagttcaa cgacactatg
    19681 cctacgtaca gggtccttgt gaacagcgat gagctggtca aggagggact gttcgccagg
    19741 gattacctaa agggaggata tacatacgac gcctatagcc ccgcgcggcg catcgactac
    19801 atatttacct cctttgacat cggcatagtg gatgaaccgg gagcggccag agttccgcag
    19861 accctcgcat ccgatcatct gccatacatt gccgccatag aagtgcccag tgccgactgg
    19921 tccgggcaga acgatatgac ggatgcggct cggactccgc tggttacggt ttttacgggc
    19981 cgggcaaatg aagagtggta tgaagacatg cgatgggatt ggcaggacca cataaacgcc
    20041 atattcgacg tggtagaaga ggcgggatac gagtgtgtcg aggttgctga ggacaacatc
    20101 gaagtccttt ggcaggtggc gcaagtgcgc cccacgacac tcatactgtc caatgcgagg
    20161 cggatgtcgg acgcccagtt gcaggcagtt cgggattttg tggcggcagg tggaagagtg
    20221 ctggcaaccg gacagacctc tcttaaatct gtagatgaga aggcgtctgg tgcccacggt
    20281 ttctacctcg ctgatctgtt tggcgtttcg ttcgtagggt ggcagggggt tgctcccttg
    20341 cacagcatca tcatgccgac aagtgacgga ggcgtactcg agtcgccaat agaggttgcc
    20401 gattctcgcg cgatcatagt aaggcgcctt ccaggcactt gcgaattggg cacatgggca
    20461 gatgacgatg gcagtcctac ccatgctgat gaatacaact ccgctgtggt gttgtcgggg
    20521 cgcgtgattt atgtaggctg tgatattttc gcgcctgaaa tgctttcaga tgatgtgaga
    20581 tcatttgtgg cagacgcatt ggatgcactt ttccagctct aactcgtgct gttgctgtac
    20641 ggaccatcag tgcaagagct ttcggaaatt gtgcaacaca acatgaaggg ttggggacgg
    20701 cgcagggcgc cgaggaacag ggggaatgtg catgccccag atgcaaaaca gcagaatccg
    20761 cattgaagta gatggaaaga ccggagaagt cagaagcata ttcaatatcg tatctcgtga
    20821 cgagttggtg aagtggcctg aggccgtgca cggcgctccc cttcggatcg tatgccgcga
    20881 tttgcaaggg cacaggcgtg tatttgctcc agggcccgcg tcaattgcca ctgcaacaaa
    20941 gtttaacggt gtggacaagt tggccttgaa cgtggagcat cgaacggtgg tggccagcgg
    21001 tgaggccata acgccgtgta ttggatctgg tggcgaggag gatgacgcgt cgggtctcct
    21061 cgggtgcggt agaggcgaag acgagatcga gattgcgtgt tcgtggagcg ttgaactgga
    21121 tgaagagaat ccggagattt cgaagtgggc attgtccgtt gataaccgct cacaagatta
    21181 cgaggtcata gaggttctct ttccgtatgt ccgcggaatc caggtaggcg tagcaccgga
    21241 ggacagcatc ctcattttcc cgcaccatgc tggcgagaag attgagaacc cggcaagtcg
    21301 gcttgcatcc gatagatata tgaagttttg gcgagcagag agccgtattg agcccgacgg
    21361 gacttattcc cgagagatca actactgtgg acttgcgtca atgatgtgga tggattgtta
    21421 cgatcggaat ggttcgggcg gactatacat ggcctcatac gacagtgact tcatactgac
    21481 cgggatcagg agcgagaccg gggggccaga tgatccctgg tgtggatttg ggttcagaaa
    21541 atacgtgccc attgggccgg gagaggtgtg gagctcgcac ccatacgttc ttggggttca
    21601 ttcaggcgac tggcactggg cttctgagca atacagatca tgggctgagg agcatttgac
    21661 tgtgactggt gtccccgacg acttggcgcg cgagtcggca atctgtccac gctacgattt
    21721 caagaacggc cagaccgtga ggaacagatt tgttgacatt ccggagatgt atgggactgc
    21781 tcaccgtgag catatcaacc attttctcat atccggctgg aacaggcagg ggtttgacac
    21841 cgattatccg gagttcatcc ccgacatgga gctgggcagc tcttggcagc tgagagaggg
    21901 ctgcaggcac gtgaggagcg agggtggatt caccaccttc tacatcaacg ttcgcctttt
    21961 cgatactgag tccaagttct tcgattccct gggaagagcg tgggccgcaa agggattgag
    22021 cggcgagatc acccatgaga tgtatgggcc tcggacattt gcagtccttt gtcccggctg
    22081 tggcgactgg caggattggg cggtcgacac tgcatcgtgg atggtcgacg ccttcggggc
    22141 aagaggcatc tacctcgatc aactggggtc ggctactccg ttgccatgct acgattcggg
    22201 ccacgagcac gtgaaggtgg ggaatcgcgg ggaacggccg catcatggcc tctataacca
    22261 cgggtacgtg aagatgatag aaaggatcaa ggagagatgc cgcctgatag atccatcatc
    22321 ctttctgatg attgagaatt gcggcgatgt ttatagccag tacctatatg caaacctgac
    22381 ctggaatggc gaggaatacg atgagttctt caacatgtac aagtacacat tccccgagtt
    22441 tatccaggta aacatggtaa atcccagacg aatcgacgac cgcgaccttc gttatgcctg
    22501 gttcttccgg gacttggccc gggcattcgt gcttgggtcg gtattctgga tggagcttgg
    22561 agaccgtttc gggcccggaa acgaagaatt gcgcgaagaa gctctggagg cgctgcgcct
    22621 gcgacaacag gctgccccct acattgctcg tggtacttat agggacgact tggggctggc
    22681 ctttatcggc gaatgcgaca ctgcgccaga tccggcgccg aggatttggt gggatgacga
    22741 ggacggttcc gagggtctgg aaggcagttt tgggcatagc tgcgattgga cgtgcttgcc
    22801 atttagtgat cttatatcgg tcactgccag tcgctggact ctggccgggg gaggagtcct
    22861 tatcctcatg tctaacccgc agaggttgaa ggggaagcgt gtctgggtgg aagacgggtg
    22921 cggagggggc gcgatttcgg gaagcaggcg ccatgcaatc cagtacgagc tcggaggtgt
    22981 gcgatccgac ctggaggttg tcatcgatga taggggagga gtcactctgg aggtccccga
    23041 gtcgaggctt tcgtttattg cagtgcttcc caaagagatg gtcataggag gacgataggc
    23101 tttgaatgcc ggagaacgcc gcgagggaag cggccctgtt gatatgcata ttcacacgaa
    23161 tgcgtctgat gggcagccta cagcggatga agtgttgaag caggctaagg cgaaggggct
    23221 ttctatggtg gccataactg atcactatga ccctttcgcg gcgaaatacg gggcccaatc
    23281 aggaaatgtg ttcgatgaca aaagcctggc ggagcagctc aagtggagag acgggcttgc
    23341 cggggaggat gatatcgagg tcttcatcgg gattgagcgg gggcccattc cggtgggaac
    23401 cgcatccctg ccgatgcagc ccgatttcgt gatcggaagc gtgcactatg ttacatcaaa
    23461 gatccccgtt gagcgtggaa ggctctacga tgagagatac tggcgcgcct acatggatga
    23521 ggtgctgagg gtagcttcag atcccgaagt ggacgtaata ggacacatgg caggctacct
    23581 gcccatgggg cagatgttgc ttcccgggtc cacctttgag gagaggcggc agatggagcg
    23641 cgagatcgat gccaggttct tctcgcgcga gtggtatgaa gacgtcttca gggcggccct
    23701 agccaacaag acggcaatcg agctgcattg tgcgacttgc acccccagga tcgatatggt
    23761 cgagttgggg ctgaagatgg ggataaagtt ctctgtgggc agcgatgccc ataatgttga
    23821 aagcgtcggc aaagtggcgt gggcatatga tactcttcag tctgtcggcg gtggacggca
    23881 caatctgtgg tgtccgcttc ctcagacaag agtcggtgcg cgcccagtcc gttagggggt
    23941 agtctaggtg ttcgagtatt ttgacgtcgt ccgatctttg ctggacgagg ttgagcgggc
    24001 tgaggctgag aatatggagg aggcagccac gctcatcgcg gaggctattg cagatgggcg
    24061 ggtgctccat tatttcgcgg ccggccattc tcacatgctc ggagaggagc tgttttacag
    24121 ggcaggggga cttgtgcctg tgaacgccat cgtcgagccc gcgctcatgg ttactttcgg
    24181 cgccggcaag agctcccaca tggagcggct tacagggctt gccaggatca tacttgatga
    24241 tgcaggagta ggcagcggag acgtcatgat cgttgcatcc aattccggag taaaccctgt
    24301 gccggtggaa atggcggccg aggccaagtc cgacggcgta aaggtgatag gcttgacgtc
    24361 tgtcgagggt tctcggacca ctccgatcag aaacagcgtt ggcaagcgac tttatgagat
    24421 cgccgacatc gtaatcgata cgcacgtgcc atggggcgat gcggctatcg aggtggaagg
    24481 ggtttcgcag aagaccggac ctgtttcgac ggtgataggg gcggcgatac tcaacactgt
    24541 tgtagtgcgg gtcatcgaga gactcgtgga acgcggaatc gacccgccta tcttcatgag
    24601 ctctaacgta ccaggcggat tggaacataa cgaggtattg cttaaacagt atagatccaa
    24661 gatcaaatcg ttttgatagg cggaaggcta gccactgttg gacgctggtt actgggctgc
    24721 gaagcccttc attgaccaaa ggttgggagg actgattgga atgctcggtt tgggcgcgaa
    24781 gtaccttgat gaactgcgaa agctgataga caggattgag aagacacagt cagaaagcat
    24841 tgctgaagct gcagacgcta ttgtagactc cgtgagtcag ggtggagctg ttcacgtctt
    24901 ggacacgggc cacatgctta tgaacgagtt gtttggccgg gcgggcggac tgatgatgcc
    24961 gagacctgtt aacatcaaga tggaagtgga caaccccggc cccgcaaggc ctggtaagac
    25021 caaggaaatg gtctatctcg acgagatacc cgggttaccg gaattcatcc tcgacagaag
    25081 cgatatctat cctggcgatg tccttatcat agggtctgta tccgggaaga acgcgctccc
    25141 cgtaggcctg gcgcttcttg caagggagag gggcataaag gttattgcgc ttacatccgt
    25201 agcctattcg tcgtcgctcg aggcgcagca tccgtccggc aaacacttgt ttgaagcagc
    25261 agacatcgtc ctcgacaact gcggcgtcat aggagatgcc atccttgaag tagagggcct
    25321 tgacaccaag gcgattccga catcgggcat tgcagcggcc tgcttgatgt ggacgctcga
    25381 ggcagaaatc atcgatagga tgctcaaacg gggcttgacg ccccaggtgt ggaagagcaa
    25441 ccatcttcct ggagcggggg agttcaacag aaaggccctt gcagaaacgg agcgggtggg
    25501 gtactgacag aggagcgaag aagaggcgaa gccccgattc gatcagcgaa tcagacggag
    25561 cagcgcgccg aatatccagg agagcgcgga caggccgagg ctgatcacta gcatcgatgc
    25621 gatgggggct attacggttt ctgtcatgaa atcccctggc agatgtccaa accaggagaa
    25681 ggcttttggg tggcgtacga ggatgaatcc gatgaccgtc atcatgccgc ctatctgaaa
    25741 tagaattgct ccaatgcgct gagccaaaaa gatcactcct caagctttgt ttttcagaca
    25801 cgatccagag ggcaagcatc cgccttgccc tctggatcaa gctcatgcat atggataagg
    25861 atgcacctgt tttagcatgt aaaggattcc gatggtgccc ggaccgcagt ggcttcctat
    25921 cacagagcct gccg
//
LOCUS       DAOSYL010000030        25760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1578_length_25760_cov_8.644427, whole genome shotgun sequence.
ACCESSION   DAOSYL010000030 DAOSYL010000000
VERSION     DAOSYL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 25760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25760
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1578_length_25760_cov_8.644427"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLU15_06445"
     CDS             <1..886
                     /locus_tag="PLU15_06445"
                     /inference="COORDINATES: protein motif:HMM:NF018155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30598.1"
                     /translation="AVTADHGLLERFVNEDGSHNLSHTCSRVPFGLVLPTGGPSVQLR
                     AAEDATLADVAPTLLSMMGVPVPEEMTGKCRAVHEGTYDRLVLVILDGWGVGMDDSTI
                     NPIAVANTPCMSRMRKECPYMTLAAAGMAVGLPKGRSGNSETGHLTLGAGRVVPQDEL
                     RISQAVGAGGLKENEAVNYGFEAARSHGGDIHVIAMLSERSSHGNMDEGIAVLRAAAG
                     KGIGRAWLHLILDGRSSPPQGAADLLEILEAKIPSGMNVEVVTAMGRAYALDRSGSYQ
                     EKTEVAYRALVMGEGRDF"
     gene            940..>1477
                     /locus_tag="PLU15_06450"
     CDS             940..>1477
                     /locus_tag="PLU15_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30599.1"
                     /translation="MNVRAGAIPALGDIIESCIRAVDRLIIVLLCEPFFPLWVIVLPH
                     RFAVAGSLEEAKARPDGQFQDLRGLDRAFCPRDAMDRTTTADTTTMDYTTMVGQAMGG
                     RIDLPTSHTQDTMHSCKQSPPAIRSNRSKTPYTPTPFVLMAPRVEDMSITGYSLRCGV
                     ELIQKALDIRNATWMPIIE"
     assembly_gap    1478..1577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1578..3425
                     /locus_tag="PLU15_06455"
     CDS             <1578..3425
                     /locus_tag="PLU15_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO30600.1"
                     /translation="WKFLLIFIVFAAIMGATAWVCMSTIDSFASDVDEMASRYIPQLE
                     NALKAQTTAVSIDGYLAQLVMAEDYDTLTYYEHKIEEAKQAHEKNMRIVQGRLKTPKG
                     RGVYDELQGLWEEYLSFVAEVESLARMGAADTARFNMNNGQVITAELEGLHDQLVACV
                     KGETDSVIERIQLNATRNLRRIVWITAIGALAGLVVGFPIGRSIAVPARRLAMLAPRV
                     AAGDLTVDANSEAADEMGVLTRAFGGMIANLRNVVSESSAMARDVAAASQELSAGSEE
                     TAASIQEVTTTVRQIAGQAQEQSYSANETALSTAELGKSIRRVHEGAETQADVVSRTS
                     AAYDEMGHSFDKTLILIDDVRLSTRRNVEAATDGCKVVDGLAARMAEIDKKTIAASER
                     ISELEGLSHEIGRIVGVIEDIADQTNLLALNAAIEAARAGEQGRGFAVVAEEVRKLAE
                     SSARETRAIGGLIQQVRESTEQAVATMKLQGREVADGNCAAQEAGKTLVDILSAAEET
                     VLAIDSMMKEIEQFRMIARQVEANMKEVLEIAQSNTDDAQNMSIHIGKVEQAVETIAA
                     AAAESAASVEEVSASTEEISAAVHRVASSAQTLAEMSARLQQSVAKFTV"
     gene            3526..5481
                     /locus_tag="PLU15_06460"
     CDS             3526..5481
                     /locus_tag="PLU15_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO30601.1"
                     /translation="MEYMISGIVGQCVERPSGMFLGVDTVKFNSLRTKLVVVITCLVL
                     AVVAITGCVFYFQSWSRVFAEIQQRILAIARTAASNIDGDEHCAVVELRMEDTYAYES
                     IQEYLRRVRNDNPDVVFVYTLAKDTDGEVLIIVDETEGEDHAEIGHLYQSNPAMERAF
                     AGAASVMEDLERDEWGTFLTAYAPIFNCTGDVAGVLAVDLDAGQVLAARKKQISAAVL
                     SAVVGVILAGAIAFALAKSIAVPVKDTVAMLKDISQGEGDLTQRLEVLTTDEIGHLAE
                     HFNVFADKLQGIIGKISITANEVATASEELAAGSGEVSKAAQQIAASTVQVAQGAQDE
                     SEHARRSVELADRMNESINKLAEDAGQQESHIEETFEVVASMISSLAETGEGLRVTSE
                     ASADNARMAGEGIENVNQVIEGIERVKETTDDVVERIEELDAYSQEIGKILEVIGDIA
                     DQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLAESSAAETKAIAELVMRVREATAG
                     SVATIRTSAEEVEKVSSLSTETGAVFNRVFEIANRTEKDIENIMGKTDQLLENSARVE
                     EVMRDLVKLAGHNRVVAEELAGLAGEVRESADNTAAVAEETAAAAEESSASVEEISAS
                     VEEMTASAESLSRMAGRLTELVGQFKV"
     gene            5850..6791
                     /locus_tag="PLU15_06465"
     CDS             5850..6791
                     /locus_tag="PLU15_06465"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO30602.1"
                     /translation="MNPNIEMLAKSAIEEMPEGIVVLDCDGRVVISNRTARSEWGVIV
                     GEDFAACAESNCSCADGGRESGYACKRLKHVVRRIKLTDGEGSRLGDVVCGFSDYESR
                     SDSSDARGYAIRNVANAMIAVISEGVEGLVKSIGVIDHQTARHSFRVRNMSVAMARMR
                     WGTGGDYRDLALAGTLHDIGKALIGEEVLAKAGSLSAEEWAEVRKHPVIGEKMLLQPA
                     AFMAQIAKAVRHHHERYDGAGYPDGLSGDAIPEMARILAIADSFDAMTSDRPYRLALL
                     RSEAIKEIAENAGTQFDPDWVEVFMELMKSERSPFMN"
     gene            6922..7125
                     /locus_tag="PLU15_06470"
     CDS             6922..7125
                     /locus_tag="PLU15_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOO30603.1"
                     /translation="MANRRPIVSDEVKYEIARELGFADKIQVGADGYDYGNITTREAG
                     LIVRSLIEKAQRAMADQLTRRGQ"
     gene            7739..8752
                     /gene="galE"
                     /locus_tag="PLU15_06475"
     CDS             7739..8752
                     /gene="galE"
                     /locus_tag="PLU15_06475"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOO30604.1"
                     /translation="MRVLAVGGAGYVGSHVVRSLAQAGHSVIVLDNLSTGHPEAVDVA
                     RRVAGGGCVELVVGDVGDGTRVRELLVSKEIDAVMHFAAASLVGESMHNPSKYWRNNV
                     GCAVELLDAMAEAKVDKIVFSSSAAVYGEPTTTPIREDHPKAPTSTYGMTKLAIEHML
                     IGYDQAYGIRSISLRYFNAAGAHGSGEMGEAHDPETHLIPIILSAALGIRENISVFGT
                     DYPTPDGTCIRDYIHVDDLAEAHLSAMDALSRGAATTAYNLGNGSGFSVLEVLREAEH
                     AVGKRIPAVHGERRAGDPAVLVASSDKAMNELGWAPHYTDISDIIASAWKWHSSHPHG
                     YLR"
     gene            9100..10353
                     /locus_tag="PLU15_06480"
     CDS             9100..10353
                     /locus_tag="PLU15_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO30605.1"
                     /translation="MTVVRAQHRRRDARVNYKLFFIDAFGYPLAFSLISTSTIMPLLL
                     TDLGASNLAIGLVPAIQSLGALMPGILVAPFLERLPLKKRLFVALGLAERLFILSMAG
                     IVAIWGVAKPSLAVKLFLLAWTLASLMAGINMTVYYAILPKCIPIDQRGGLFGWAGAL
                     SGVFGIIGAETAGYILETVEFPRNFALLFIMAFVILIASLVQFIWLREPPDEKSEGSR
                     SIKEYIAAASYEVREDRAYVWTIAALAVMAFAMSATSFYSTYAVRLFGAGTRDVGRLT
                     AFAVGATIVGMPALGWIADREGHKLSLEITAACFACGAALALAANNVTEMYPAVVFAN
                     IGMSGIAVSHNLILAEFAPTHTDVPMYTAFSWMLLAPFRTGAPILGGYISDIAGFPAM
                     FSISLAAGIAAVCIFLFAVEEPRRA"
     gene            complement(10447..11820)
                     /locus_tag="PLU15_06485"
     CDS             complement(10447..11820)
                     /locus_tag="PLU15_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HOO30606.1"
                     /translation="MVHNFAWKGIVEGFYGRPWTHKERLDMISFISEVGMNAYMYAPK
                     DDPYHRENWRNPYPSRKLESLRELASHAKSHGISFVFAVSPGMSVRYSDKRDIDAFMQ
                     KLKALYGAGVRDFAILYDDIPNALSNEADREKFPNLASAQVCFANSVWERLKKWSGDN
                     TLMVCPTQYNGDYNTEYVLGIGAGLHQDIDIMWTGPDVCSRELTYEYTQAVTAALMRP
                     VVYWDNYPVNDAGMRGELHIGPYTGRDRRLPEVCRGFFLNPMNQAEASKIALGAAASY
                     LRNPEGYDPQAAWEAAAVRVLGEDALEAITTFADACAISPLHPEDPPMLKEAVDRAVE
                     RATENFQEGAGILSAYMLKMKASAELLRTNPNTKFVEEARPWLEEYVRWTDIGMHIAE
                     TVEKSGAYLVSSASSGRRGFSLHALPIVSARRKLSRMMSDAVNFKTRVCGDVLLQLGR
                     DLLRQLQ"
     gene            12061..13461
                     /gene="lysA"
                     /locus_tag="PLU15_06490"
     CDS             12061..13461
                     /gene="lysA"
                     /locus_tag="PLU15_06490"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOO30607.1"
                     /translation="MTYNRYDNIEANGKGHLTIGGCDAVALARRYGTPLYVVDEMAVR
                     NACRAHRREFESRYPRVRILYASKALMTKAIAMIVAEEGLSLDVCSGGELYTALSAGC
                     PPDRIYFHGNSKTLSEIDFALSAGICRFVVDNMDELRLIDLLANAKGLVADVILRLTP
                     GIEAHTHEYIKTGQIDSKFGIAIPNGDALRAVKYAKELRNVKLTGLHCHVGSQIFETE
                     PFLHAIEVMMDFAREACDATGFIMEELDIGGGLGVRYTGGDDPLSVADLAEAIAPALL
                     KAAEERCLPLPTLLMEPGRAIVGEAGTTLYTVNAVKHIEGVRTYVTVDGGMADNPRVA
                     LYQAEYDAVLANKADRPPTCEVSIAGNCCESGDMLAWDIALPDVEVGDILAIFCTGAY
                     NYSMASNYNRHLRPAMALVKDGWSDLIVERETYEDLVRKDVVPDRLKSSHRSAAVSAE
                     SAMLVYPRASGANASE"
     gene            13516..14394
                     /locus_tag="PLU15_06495"
     CDS             13516..14394
                     /locus_tag="PLU15_06495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO30608.1"
                     /translation="MNVRDEPANRICEVEDEYAFLGCNALRGDGYTAVLSDICPDMRD
                     SNHVRQINLPRAADPEGFISRAEELFRSKGREHCRFDLDCRTRPQCLYTALRKSGYVP
                     HPTVVQVYRGHDAEGAGGVFSGLSGGERDDRQSDFHPSASGSEEWRNLMREHYAGYGQ
                     CGAFVERAVDLAIHRLAAAWGEGRDYRVFLAFAGDDSDASGVCEIFRHKGVAKIERLY
                     VRKNSRMQGIGSSMIMHAVDYALRAGDDLAYLVADSLDWPRRMYRKLGFEDLMEIVEW
                     TRIWGGSADDEGYVTW"
     gene            14703..14924
                     /locus_tag="PLU15_06500"
     CDS             14703..14924
                     /locus_tag="PLU15_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOO30609.1"
                     /translation="MREKVEALLDEIRPSLQADGGDVELVDVTEDGIVKVRLKGACAG
                     CPMSTLTIKNGIERVLKNEIPEVKAVQSV"
     gene            complement(14996..16168)
                     /locus_tag="PLU15_06505"
     CDS             complement(14996..16168)
                     /locus_tag="PLU15_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOO30610.1"
                     /translation="MAALVLTGIICAVVFSKGYSPQTPETPNGFRSEPANAEESAVWP
                     GSRKLSQDHESAAGAVVPIDDGGGASPESESEPRSATLCECGDLHKLWLCEPRMVGKQ
                     VRDLQEGLAVAGFPAGHVDGLFGPATEGAVRAFQASVGLSEDGIVDLSVWMTLGRVCE
                     QIVAASLEPSGDIIPIAKTSPAKPQTEQEGTTRKGLPPGWRALLIDTSNLTITLLEDG
                     LPIKQYRVGVGEPSTPTPLGQFKVIEKAAWAGGFGTRWMGLNVPWGKYGIHGTNKPWT
                     VGQRKSGGCIRMLNRDVEELYSMVPVGTPVVITAGHFGTLGSFRPRIEPGAKGSYVLE
                     VQKRLAKLGYEHAGFDGVYGPGTERAIMQLQRDKGLPVTGIVDMRTYEALGLFIFD"
     gene            16378..17418
                     /gene="tdh"
                     /locus_tag="PLU15_06510"
     CDS             16378..17418
                     /gene="tdh"
                     /locus_tag="PLU15_06510"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOO30611.1"
                     /translation="MRGEMRAISKMRPEPGAELITAPIPQPGRGEVLVRVRATAICGT
                     DVHIYEWDPWSQARVTTPRIFGHEFCGDVVEVGEGVTKVKEGQFVSVETHTACGKCFY
                     CLTGLAHICQNAKIMGIDRDGCFAEYVVVPEFNCWIWDIDISDEVAAIQDPFGNAVHT
                     TLATDLVGKTVLITGMGPIGLCAISVAKAAGATEVYVTDVSDYRLDLACKLGADLAIN
                     PKREDPVAAVREATGGVGADVLLEMSGSPSAIVQGFDALRKAGFASLLGIPSEPIKLD
                     LANAIIFKSATVQGINGRRMFETWFQARSLLKAGLDITPVITHKMSLWDDLDEGMELM
                     MSGQCGKVILYP"
     gene            17538..18410
                     /locus_tag="PLU15_06515"
     CDS             17538..18410
                     /locus_tag="PLU15_06515"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOO30612.1"
                     /translation="MPEVIYSEEKLKAQDGVELFCRVWKGVSLEGEPALKARSTIVIA
                     HGLADHSGRYEHVAKFFAQKGYVVYAYDHRGQGKSEGVRGHVNKFSQFYDDLWFVIRR
                     AKGRNPGKKCFLVGHSMGGLIALGYAARRPKTINGLIVTSPCLALRMRVSPIKKGFGT
                     FISWLFPKMAVDTGIAAEGLSHDSNVVEAYKKDPIRYPKITTKFYVEFDKAMKNVQSL
                     AKSIVTPCLFMPGGDDPICSTEATIKFYENVPETTDKKLIVWDGLYHEIMNEPEKDKV
                     LDTMAQWIAEREGK"
     gene            18410..19321
                     /locus_tag="PLU15_06520"
     CDS             18410..19321
                     /locus_tag="PLU15_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOO30613.1"
                     /translation="MPALSSAERQRYSRQIRLARVGEVGQGRIRAARVLIVGVGGLGS
                     PIALYLAAAGVGVIGLVDPDVVELSNLQRQIAHTTCDIGRLKVDSAERRILALNSEVQ
                     VEKWAVALDVDNAGDLVGRYDIVVDALDSTGARLVLNDACVAAGKPLVHGAVLGWTGE
                     AMTYVPGCGGPCYRCVFGGAVVEEGAQCWVGDDDACTAIRHDDGAHHGRSEEESLGQL
                     AGAQAKDDRGNGLEKLGPFDPSRVGVLATVPAVIGSIEATEVLKLITGAGDLLVGRLL
                     LWDGLEMRFDEVKVAADPECGACGIDG"
     gene            complement(19893..20798)
                     /locus_tag="PLU15_06525"
     CDS             complement(19893..20798)
                     /locus_tag="PLU15_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30614.1"
                     /translation="MISRRGIVIIVLIGLAVVASSTHPWVSGSNLLADKYSSMFDPYI
                     NTDTGIIAKAQFEEAFSAAGIKCVAIQVPVGDITVIKTGGNNITIDGERIAYGETLEK
                     AETKLSLVPLEARTRDDVLEIISLVDTSATTGSSSRINMVIQVPAGIDVRSTLSAGEI
                     TLNGIDGAIYAKVDTGKVNTDSTRGDLDIEVSVGAIDVRDAVVANKLSLITSMGPIDF
                     LGTLGKNNYIESSAGPIKISVPRSTCVRIDAAISAGQINSSLPITGLNSDNQKSSATG
                     ILGNGTPTGELKVRASMGEISISEL"
     gene            complement(20838..21620)
                     /locus_tag="PLU15_06530"
     CDS             complement(20838..21620)
                     /locus_tag="PLU15_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30615.1"
                     /translation="MKRWWSLVGKEWRGSWTVILLALAATIGFDTWLAIKAPVWSARI
                     NGGLAFGLSFIPFVGMGIAALVVGYYTMRNEWRDRTSLQLLSYPVSGAGILWAKLLVA
                     VLGLGLITVVATVGTWFVTQRSSIPYSTIMSATEDILRLTPWERTLDIIWMVVCSILG
                     TAFIVAVGLFSFTIGTIVPRISGLIAVIVYGLTWYLTFAFAPHAMYAFSFVPNPGLIV
                     YTGLGPMAARKVIPLLPFWPTIMGTVILILIAGKILELEVDA"
     gene            complement(21622..22314)
                     /locus_tag="PLU15_06535"
     CDS             complement(21622..22314)
                     /locus_tag="PLU15_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30616.1"
                     /translation="MPAIELTDVHKRYYRSHALRGVNLSVDRGKIVGLLGPNGSGKST
                     LLRICAGLASPTSGKVQVHGQDISIATKAITAYIPDIDSFYSWMTVRQAIAFESSMFD
                     DFVLADAMDLVRELKLDPDNKISSLSKGQRGRLKLVLAMSRRAELVLMDEPLSGIDPP
                     SRTAILETIADRYRAGEQTIIISTHEVLESEKLFEDVIFLSEGRIVLTGEADQLRAER
                     GKSLNEIFAEVC"
     gene            complement(22314..22751)
                     /locus_tag="PLU15_06540"
     CDS             complement(22314..22751)
                     /locus_tag="PLU15_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO30617.1"
                     /translation="MGISFDESVPIYTQIMKSVLWDVATGALLPGDKLLSVRDFAKQA
                     KTNPNTVQRAYAELERMGVVETQRGQGTFVSASPEIAREVRSEMTTAAVESFVEEMRR
                     LRYSDSDIISLVSQQLAKTGTNSSNGAAQNGPSTTNDHDGGVQ"
     gene            23068..23144
                     /locus_tag="PLU15_06545"
     tRNA            23068..23144
                     /locus_tag="PLU15_06545"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23102..23104,aa:Met,seq:cat)
     gene            complement(23386..25158)
                     /locus_tag="PLU15_06550"
     CDS             complement(23386..25158)
                     /locus_tag="PLU15_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO30618.1"
                     /translation="MDAGPKRSAATGPNGTFTLSNVPAGRVILNASCPGYRPRQVNVT
                     VPAGNSINCEIELYPNTSGLATVRGCATVANNYYPEEDQSNHVLSAKTASRSLSAFQK
                     APLGQQPAPETEADTIQVKVRPGTDRDELAHNLLQAGYEIVDELPLTGIILIRPMQRT
                     ARFLVESEAATLDSIDGVDWAYPDYPVYAAAVPNDPLYLYQWHYPAISLPEAWDIATG
                     EGSPVIVAVIDTGIRPEHEDLQGKIVAGWDFIDKDSDPTDWPNSTKKYSHGTHVSGTI
                     AAATNNGVGVAGVSWGAQIMPIRVLGPDGKGSSSNVAEAIIWAVNSGADVINLSLGLA
                     NHPGSAMEDAVRYAFSRGVTMVAAAGNDSGAPVLYPAAFPEVIAVSATGQNNELASYS
                     SYGPEITVAAPGGTALFPVLSTGYSSANPAPNKYFLSQGTSMATPHVSGIAALLIATH
                     GRMSPASIAELISATSMDLGPDGRDHEFGAGLVNAYAALVGATIDRAVFAIKDNAGKW
                     ITDSVQGTRDRTFTIFSAPAGDHVLVGFLDVDGNGLISKGDFYGEAPITIPIAGTVYP
                     PRLVLNYVDGATATKRFTTSSRLP"
     gene            25187..25441
                     /locus_tag="PLU15_06555"
     CDS             25187..25441
                     /locus_tag="PLU15_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30619.1"
                     /translation="MASLTVPMIEPVFGGEGLPMQPLRKMQAKAVMRRYVMLRCACKA
                     MPPTSVAKGKDHVLEIFEDEGLDPLGESRKCLGDRTRPSF"
BASE COUNT         5707 a         6257 c         7741 g         5955 t
ORIGIN      
        1 tgctgtaact gcagatcatg ggctgctcga gaggtttgtg aatgaagatg ggagccacaa
       61 ccttagccat acatgtagtc gtgtgccgtt cggcttggtg ttgccgaccg gagggccgtc
      121 tgtgcaactg agggcggcgg aggatgccac ccttgcagat gttgcgccca cgctgttgag
      181 catgatgggc gtacctgtgc ccgaggagat gaccggcaag tgccgcgccg tccatgaggg
      241 aacttatgat cgactggtat tggttatttt ggatggatgg ggagttggca tggatgattc
      301 aaccatcaac ccgattgcag ttgccaacac gccgtgtatg agcagaatga ggaaggaatg
      361 tccatatatg actctggctg cggctgggat ggccgttggt ttgccaaagg gaaggtcagg
      421 caattcggag accgggcatc taactctcgg cgccgggcgg gttgttcccc aagacgaact
      481 tcggatttcg caggctgttg gcgctggagg gctcaaagag aatgaggcag tcaattacgg
      541 ctttgaagcg gcgcggagcc atggcggcga tattcatgta atagctatgc tgtcggaacg
      601 gagctctcac gggaatatgg acgaagggat tgcagtgctc agagcggcag cggggaaagg
      661 aattggacgg gcttggctcc atctgatcct tgatggtcga agcagtccgc cgcagggtgc
      721 tgctgatcta ctagagattc ttgaggcaaa gatcccgtca gggatgaatg tagaagtggt
      781 aacggcgatg gggcgtgcat atgccttgga tcgcagtgga agctatcagg aaaagacaga
      841 ggttgcctac agggctctcg ttatgggaga gggccgcgat ttctagctta acgtccgcct
      901 aacccgtaaa tgttgagaac agcagaatga tgccggctat tgaatgtgag ggcaggggca
      961 atccctgccc tcggcgacat tattgagtcg tgtatccgtg ctgttgacag actaatcatt
     1021 gtcttgctgt gcgaaccgtt ctttccgctc tgggttattg ttctacctca tcgcttcgcg
     1081 gtcgctggaa gtctggagga agcgaaggcc cgcccggatg gccagttcca ggacctccgg
     1141 ggcctggacc gggcgttctg ccccagggat gccatggacc gtaccaccac ggcggatacc
     1201 accaccatgg actataccac catggtgggc caggccatgg gcggccgaat tgatctgccc
     1261 acaagtcata cccaggatac gatgcacagt tgcaagcagt ctcctccggc aatacgtagt
     1321 aatcgtagta aaacgccata tacacccact ccgttcgtgc ttatggctcc tcgtgttgag
     1381 gatatgtcta tcacaggata ttccctgcgg tgtggcgtgg aactgataca gaaagcattg
     1441 gacataagga atgccacatg gatgccgata atagaaannn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnntgg aagttcctcc tgattttcat cgtatttgcg gccattatgg
     1621 gagctacagc gtgggtatgt atgtcgacga tagactcttt cgcttcggat gttgacgaga
     1681 tggccagcag atatattccg cagctagaaa acgcgctcaa ggctcaaacg acggctgtga
     1741 gtattgacgg ttaccttgct cagcttgtga tggccgagga ttacgatacg cttacatatt
     1801 acgaacacaa gatagaagag gctaagcagg cccatgaaaa gaacatgaga atagttcagg
     1861 gacgactcaa aaccccaaag ggacgtgggg tttatgatga attgcagggc ctatgggagg
     1921 aatatctctc tttcgttgca gaggtggagt ctcttgcaag gatgggcgcg gcagatacgg
     1981 cgagatttaa catgaacaat ggacaagtta tcacggctga actggaaggt ctccatgatc
     2041 agcttgttgc atgcgtcaaa ggagaaacag acagcgttat cgagagaatc cagcttaacg
     2101 ctactcgcaa ccttcgccgg atcgtctgga tcacagcgat tggtgccctc gcaggtctcg
     2161 ttgtcgggtt tcctatagga cgttctattg cggtgccggc caggagactg gcgatgcttg
     2221 cgccgagagt ggccgcgggc gatttgactg tagatgcgaa ttcagaagct gcggacgaaa
     2281 tgggcgtgct aacgcgggcc ttcggcggca tgattgccaa cttgcgcaac gttgtttctg
     2341 aatcttctgc aatggctcgc gatgtcgccg cagccagcca ggagctttcg gcgggctcgg
     2401 aagaaacggc ggcgtcaatt caggaagtga ccacaacagt acggcaaatt gccggtcagg
     2461 ctcaggaaca gtcgtactcg gcgaatgaga ctgctttgtc cacagctgaa ttaggaaagt
     2521 ctatcagacg tgtacatgaa ggagctgaga ctcaggccga cgttgtgagc cgtacatctg
     2581 ctgcgtacga cgagatgggc cattcgtttg acaagacgtt gatcctaatt gacgacgtgc
     2641 gtctgtcaac caggcgcaat gttgaggccg ctactgacgg gtgcaaagtg gtggatggat
     2701 tggcagcaag gatggccgag atcgacaaga agacgattgc cgcgtctgaa agaatcagcg
     2761 aactcgaagg actttcacac gagatcggaa ggatcgttgg cgtgatagag gatattgcgg
     2821 accagaccaa ccttttggcg ctcaacgccg ccatagaggc agcgagggca ggcgaacaag
     2881 gtcgcgggtt cgcagttgtg gctgaagaag ttcgaaagct tgccgaaagc tctgcaagag
     2941 agacaagggc tattggaggc ctcattcagc aagttagaga gtcgacagaa caggccgttg
     3001 ctaccatgaa actgcagggg cgggaggttg cggacgggaa ttgtgctgcc caagaggccg
     3061 gcaagacgct tgttgacata ttgagcgccg ccgaagagac tgtcttggcc attgatagca
     3121 tgatgaaaga gattgaacag ttcaggatga ttgctaggca ggtggaagcg aacatgaagg
     3181 aggtactcga gattgctcag tccaataccg atgatgcaca gaacatgagt attcacatcg
     3241 gcaaagtcga acaggcagta gaaacgattg cggctgcagc tgcagagagc gcggcatcgg
     3301 tagaagaagt ctcggcttcg actgaggaga tcagcgcagc tgtacatcgg gtggcgtctt
     3361 ctgcccaaac ccttgccgaa atgtccgccc gcctgcagca aagcgttgct aaattcacgg
     3421 tgtagtcggc gcaacccgca tgtcctttac cgcaggggaa tttgtgtcgc aaaaggcggt
     3481 cttgatctgc tgcggcaatt gatgcctggt tggcgggtat gcccgatgga atatatgatt
     3541 tcgggaattg taggccagtg cgtcgaaagg ccttcaggga tgtttttggg ggtagataca
     3601 gtgaaattca attcactcag gaccaagctg gttgttgtga ttacctgctt ggttttggct
     3661 gttgtggcta tcacgggatg tgtattctat tttcaaagct ggtcacgggt gtttgccgag
     3721 atacagcagc gcatactggc catagcccga accgcggctt cgaacataga tggcgacgaa
     3781 cactgtgccg ttgttgagct tcgaatggaa gatacctatg cgtatgagtc gattcaggag
     3841 tacctgagga gagtgcgaaa cgataatccg gacgtggttt tcgtgtacac tctggcgaag
     3901 gatacggatg gcgaggtact cataattgtt gacgagactg agggtgagga ccacgcggag
     3961 attgggcacc tgtatcaatc taaccccgct atggagcggg cttttgccgg cgccgcctct
     4021 gtgatggagg atcttgagag agacgagtgg ggtacctttc ttacagccta tgctcctatc
     4081 ttcaactgca caggtgatgt cgccggcgtg cttgctgtcg atctggacgc cggccaagtg
     4141 ctggcggcac gcaagaagca gatatcggcg gcggttttga gtgctgttgt tggagtaatt
     4201 cttgccggag ccatagcctt tgcactggca aaaagcattg ctgtgcctgt gaaggacacg
     4261 gtagcaatgt tgaaagacat ttcccagggg gagggagacc tcacacaacg tcttgaggta
     4321 ttgacaacgg atgaaatcgg ccatttggcc gagcacttca acgtattcgc cgacaagctg
     4381 cagggcataa tcggtaagat atcaattacg gccaacgaag tcgccacggc cagcgaggag
     4441 ctggcggcag gatcggggga agtgtcgaaa gcggcacagc aaattgcagc atctacagtc
     4501 caggttgccc agggagctca ggatgaaagc gagcatgcca ggcgcagcgt agagcttgct
     4561 gacagaatga atgagtccat aaacaagttg gcggaggatg ctgggcaaca ggagagccat
     4621 atagaggaga cattcgaagt agtggcgagc atgatctcat cactggcaga gacaggcgag
     4681 ggattgcgcg ttacgtcaga ggcttctgcc gataatgcca gaatggccgg tgaaggcata
     4741 gaaaatgtga accaggtcat cgagggcata gagcgcgtca aggagactac cgatgacgtg
     4801 gtggagcgaa tagaagaact cgacgcctac tctcaggaga tcggcaagat cctcgaagtg
     4861 ataggcgaca tagcggacca gactaacctc ttagctttaa acgccgcaat agaagctgca
     4921 agagcaggcg aacacggacg cggcttcgcg gtcgtggcag acgaagtcag gaagcttgcc
     4981 gaaagctcgg ctgctgagac caaggcgatc gctgaattgg tgatgagggt cagggaggct
     5041 accgccggat cggtggcgac gataaggacc agtgcggaag aggtggaaaa ggtaagttcc
     5101 ctctccacag aaacgggcgc cgtgttcaac agggtttttg agatcgcgaa tcggacggaa
     5161 aaggacatag agaacataat gggcaaaact gatcagctcc tcgagaacag tgcccgagtg
     5221 gaagaggtta tgagagatct ggttaaattg gccggccaca atagggtcgt agccgaggag
     5281 cttgcaggct tggcaggtga ggtgcgcgag tctgcagata atactgctgc agttgccgaa
     5341 gagactgctg cagccgcaga agaatcttcg gcgtcggtcg aggaaatcag cgcatcggtt
     5401 gaagagatga cggcttcagc tgagtctctt tcgaggatgg ctggcagatt gaccgagctt
     5461 gtggggcagt tcaaggtctg aagaaagcac gcctgaaagg tagcgagatt acggacattt
     5521 ggccctaggc catgcagtgc aggatggttt tccagcctgc actgctattt cggtggaggt
     5581 tcgcgactgg aaacaacata ggatatgtgg agtataagct tggaaaccac gccctatatt
     5641 gacaccattg gtttggagtt ctatacttct ttgttggtat ttgaatttgt catgccgata
     5701 ttccgttcca ggttggtcgg agcgcagatt tatggtgtgc tccctgagtt ggtgttggcg
     5761 ggcgactgaa tttccgcaaa tgaagcgcca ttgcacactc ctggtcggtt tgatatagag
     5821 caccatttct ttcaggaggt tgcccgcagt tgaatccgaa catagaaatg ttggcaaaaa
     5881 gcgcgatcga ggagatgccg gagggtattg tagtgcttga ctgcgacggg agggttgtga
     5941 tttcgaaccg aacggccaga agtgagtggg gggtcatagt cggggaggat ttcgcagctt
     6001 gcgccgaatc caattgttct tgtgcagatg gcggcagaga atctggctat gcttgcaaga
     6061 ggctcaagca cgtggtcaga cggatcaagc tgaccgatgg cgaaggttcc aggttgggcg
     6121 atgttgtatg cggcttttca gactatgaga gtagaagcga ttccagtgat gccagaggct
     6181 acgcgatcag gaatgttgct aacgccatga ttgctgtcat ttcagaaggc gtggaagggc
     6241 tggtaaagtc cataggcgtg atcgatcacc agaccgcacg acactctttt cgagtgcgaa
     6301 acatgtctgt ggcaatggcc agaatgaggt ggggaacggg cggagactac cgagatttgg
     6361 ctttggctgg tacactgcac gacataggaa aagcgttgat tggggaagag gtattggcga
     6421 aggccggatc gctttctgct gaggaatggg ctgaggtgcg gaagcacccc gttatcggag
     6481 agaaaatgct tctgcaacct gccgctttta tggcgcaaat tgcaaaagcc gtaaggcatc
     6541 accatgaaag gtatgatgga gcaggctacc cagacgggct ttctggggac gctatacccg
     6601 aaatggccag gatacttgct atagcagaca gctttgacgc tatgacatcg gaccgcccct
     6661 atcgcctagc tctgcttcgt tccgaggcta tcaaagaaat tgccgaaaat gcggggactc
     6721 agttcgatcc tgactgggtt gaagttttca tggaacttat gaaaagtgaa agaagcccat
     6781 ttatgaactg atatggcgtt tgacggttgt ctgcaggaat tcaggctgct tggaggttgc
     6841 ggcgaatgcc gccactttac ccagcagcat gcctgttttt ctgggacgtt tgcgtggaac
     6901 catactcttg gggtgattgc cttggctaat cgtcgtccca tagtttcaga cgaagtcaaa
     6961 tacgaaatcg ctcgagaact tggcttcgcc gacaaaatcc aagtgggcgc cgacgggtat
     7021 gactatggta acatcactac ccgcgaagct ggtttgatcg tccgaagcct tatcgagaaa
     7081 gcgcagcggg cgatggctga tcagctgacg aggcgggggc aatagccccc gccccaaaca
     7141 tttgctgatg aacagtgtct gtgccacgtg acataggagc cattgggatg gaatcctgcc
     7201 agtgccatcg cgtttggcca gtgaaggtgt cttctagtgc cttcagtttc ggttttacgc
     7261 tagaaagaaa ggctgcaaag atagcagctt tttacatatc agaagccgct tccctcgcct
     7321 ttcacatggc caaagcgtaa agcagcgagg ctacaggatt attgcagggt catcttgcca
     7381 aagttctcca ggaactgatt gttcaggcgt atccccaaaa gcctttgagg cccggacaag
     7441 aagccaaggt ctaagcggag gttggaaacg aacctagcca acttccagtc acatcgcgat
     7501 cgcaagtgct caaggtttgg acagaggctg actaaaagtt ggtcgctctt cccctaaaac
     7561 ccgcttgcgc cgacaattca atctacggtg caaagcaacc tcgaatccgg ctttggttcc
     7621 ctgggcggtg cccgcgcgag ggtcgtcagc gccgccatga attgcctggc agtgagaagg
     7681 tgagaatgat cctcctgtcg aagtgcactc tgtgtttctt ttgaaggggg attttgcttt
     7741 gagagtgctt gcagttggag gcgcgggata tgtgggaagc catgttgttc gcagcctggc
     7801 tcaagcgggt cactcggtga ttgttctcga caatctgtcc actgggcatc cggaggccgt
     7861 ggacgttgct cgaagggtgg cgggcggggg atgcgtggaa cttgttgttg gcgatgttgg
     7921 cgatgggacc cgggtacgcg agcttctcgt ctcgaaggag atcgacgctg tcatgcattt
     7981 cgcagcagcc agtttagtgg gagaatccat gcacaacccg tcgaagtatt ggcgcaacaa
     8041 cgttgggtgc gccgtcgagc tcttagatgc aatggcggag gctaaagtcg ataagatagt
     8101 cttttcgtcg agtgcggccg tatatgggga gcctactaca acgcctatcc gcgaagacca
     8161 tcccaaagct ccaacgagca cgtatggcat gacgaagctc gccatagaac acatgcttat
     8221 cggctacgac caggcttatg ggataaggtc catatccctt cgctatttta acgcggcggg
     8281 cgcgcatggc tctggagaga tgggggaggc ccatgaccct gaaacccatc tcatccccat
     8341 catcctttcg gctgctctag gcattcgtga gaacataagc gtgttcggga ctgactaccc
     8401 gaccccagat gggacttgta tccgtgacta tatccacgta gacgatcttg ccgaagcgca
     8461 tctttctgcg atggacgcgc tgtcgcgcgg ggccgcaact acggcgtaca atcttggcaa
     8521 cgggtcaggt ttttctgtgc tcgaggttct tcgggaagca gaacacgcag tgggcaagcg
     8581 cattcctgca gtccatggcg aaaggcgcgc gggcgacccg gccgttctgg ttgcctcttc
     8641 cgacaaggcc atgaatgagc tcggatgggc gccacattat acggacatta gcgatatcat
     8701 agcctcggcc tggaagtggc atagcagcca cccgcatgga tatttgcgat gacgaaagaa
     8761 gacgggcaat tcggctgcga tgcggacgcg acttgcccgt tttatccacg aagggacgct
     8821 tttgtcctgg ggtagacggt tggaggcaat gcaaaactga atgaggttgg gatgcactcg
     8881 ctgttagggc ttgggaagtg cccaccttca cacgccttta tacattagtg tataatacat
     8941 cttgggccca cggccttcgc ggtcgcgatg ggcttttttt cttgataatc ccactgcaac
     9001 gaagcgggtg gggccaaaaa gagcatgagc cttgcgttct tgttatgttg ctgctgttct
     9061 tgcaagaata gttgtacatg atttaggagt gaataatgct tgactgttgt tagggcccaa
     9121 catcgccgcc gggatgcaag ggttaactat aagctgtttt tcatagatgc ctttgggtat
     9181 cccttggcgt tctctctaat atcgactagc acgataatgc cacttctact taccgatttg
     9241 ggtgcgtcca accttgccat tgggcttgtg cctgccattc agagccttgg cgccctgatg
     9301 cctggcatac tcgtcgctcc atttctggaa cggttgcccc tcaaaaagag gctcttcgtt
     9361 gcgcttggcc tggcggagcg gttgtttatt ctctccatgg caggaatagt ggcgatatgg
     9421 ggcgttgcga agccttctct tgccgtgaaa ctgttcctgt tggcttggac ccttgccagc
     9481 ttgatggcgg ggatcaacat gacggtatac tatgccattc ttccaaaatg cattcccatt
     9541 gatcagaggg gagggctttt tggatgggcg ggcgcgttgt cgggagtatt cggcataatc
     9601 ggcgctgaaa ccgcaggcta catcctggag acggttgagt ttccgagaaa tttcgcgctg
     9661 ctgttcatca tggccttcgt gattctcatt gcatcgcttg tgcagttcat atggcttcgc
     9721 gaacctccgg atgagaagtc tgaaggatct agatcaatca aggagtatat tgccgctgca
     9781 tcttacgagg tgcgggagga cagagcctac gtctggacga tcgcagcctt ggccgtgatg
     9841 gcctttgcca tgtcggcgac atcgttctac tcgacatacg cggtcagact tttcggagca
     9901 ggcacgagag atgttggaag gctaacagct ttcgctgtcg gggccactat cgtaggcatg
     9961 cccgcattgg gctggatagc cgatagagag ggacacaaac tgagcctgga gattacggca
    10021 gcctgtttcg catgtggcgc agcgctcgcc cttgccgcca acaacgtgac ggagatgtac
    10081 ccggcggtag tgttcgccaa catcggaatg agcggaatag cggtgagcca caatctcatc
    10141 cttgccgagt ttgcgcccac ccacactgat gtgccaatgt acaccgcgtt ttcctggatg
    10201 ctgcttgccc cgttcaggac tggcgcacca atccttggag gatacatctc cgacattgca
    10261 ggatttccgg cgatgttctc catatcgctc gcagccggga tagcagccgt ctgtatcttt
    10321 ctttttgcgg tagaagaacc gcgccgcgcc tgatgcccaa agcctagatc atgcaagatg
    10381 cctcaccagg tctgctttcg ggtgaaggcg cagcgcagcc cggtgcgcct gtgtcggcat
    10441 ccgaggtcat tgcaattgcc tcaacaggtc gcgaccgagc tggagcagga catccccgca
    10501 caccctggtc ttgaaattca cggcatccga catcattctc gacagcttgc gtctggcaga
    10561 gactattggc aacgcgtgca gcgagaaacc gcgacgtccg gatgacgccg atgatacaag
    10621 ataggctccg gatttttcga cggtttctgc tatatgcata ccgatgtctg tccaccgaac
    10681 gtattcttcc agccacggac gggcttcctc aacgaacttc gtgtttgggt ttgtgcgcag
    10741 caactccgca gacgccttca ttttgagcat gtatgcggaa aggattccgg ctccctcttg
    10801 aaaattctca gtggcgcgtt ccacggcccg atctacggct tctttgagca tcggaggatc
    10861 ctcgggatgc aatggactga tggcgcatgc atccgcaaaa gtggtgatcg cctccaaagc
    10921 atcctctccc aataccctca cagctgcagc ctcccaagcc gcctgcgggt cgtatccttc
    10981 gggattgcga agataagatg cggccgcgcc tagggcgatc ttcgacgctt ctgcctgatt
    11041 catcggattg aggaaaaatc cacggcatac ttcaggcagt cttcgatctc tgccggtgta
    11101 tgggccgata tggagttcgc ctctcatccc cgcatcgttc acgggatagt tgtcccagta
    11161 gactactggg cgcatcaagg ctgctgttac cgcctgcgta tactcgtaag tgagctcgcg
    11221 ggagcaaacg tccggtcctg tccacatgat gtctatgtct tgatgtagtc cagcgccgat
    11281 tcccagaaca tattcggtgt tgtaatcgcc gttgtactga gtgggacaga ccatgagcgt
    11341 gttgtctccg gaccacttct tgagtcgctc ccaaacggaa ttggcgaagc agacctgggc
    11401 agaggccaag ttcgggaatt tctcgcgatc agcctcgttg gagagagcat ttggaatatc
    11461 gtcatagagg atcgcaaaat cgcggacgcc tgctccgtaa agggccttca gcttctgcat
    11521 gaaggcgtcg atatcgcgct tgtcggaata tcgaactgac attcccgggc tcaccgcgaa
    11581 tacaaaggag atgccatgcg atttggcgtg ggacgccagt tcacgcaggc tctctaattt
    11641 tctcgaagga tatggattgc gccagttttc cctgtgatag gggtcgtcct tcggggcgta
    11701 catgtaggcg ttcatgccta cctccgatat gaaggatatc atgtcaaggc gctctttatg
    11761 ggtccatggt ctcccataga agccctcgac aatccctttc catgcaaaat tatgaaccaa
    11821 gccaacattc ctccttgcct acagttgcgt gataggctat tgagaaaatt caaggcaaac
    11881 tcgggttatc cttcccgtgg gctgttagtt tactctgccg tggggcacag actttgtctt
    11941 taaacacaga caacaaacgc gggcttcgca aaatactctt gcaacaacgc gtcgcatatg
    12001 ttagtgttaa cctaatttag ctggagctat tacagtggcg ccgacgggag agtgcagcag
    12061 ttgacctata acagatatga taacatcgag gcaaacggca aaggacactt gacaatagga
    12121 gggtgtgacg cagtggcttt ggccaggcgc tacggcactc cgctttacgt cgtagacgaa
    12181 atggcggtgc gcaacgcttg ccgtgctcat cgccgtgagt tcgagtccag gtatccacgg
    12241 gttcggatat tgtatgcaag caaagcactg atgacgaagg ctattgcgat gatagttgcc
    12301 gaagagggat tgtctctcga tgtctgttcc ggcggcgaat tgtatacagc tctttctgcc
    12361 ggttgtccgc ctgaccgcat atacttccac ggcaacagca agacactgag cgagatcgat
    12421 tttgcccttt ctgcagggat ttgccgtttt gttgttgaca acatggatga actccgcctc
    12481 atagacctgt tggcgaatgc caaagggctt gtggccgatg tgatcctcag attgactccg
    12541 ggaatcgagg cgcacactca cgaatacata aagaccgggc agatagattc gaagtttgga
    12601 atcgccatac ccaatggcga cgctcttcgg gcagttaaat acgctaagga actgaggaac
    12661 gtgaagctga cggggctcca ctgccacgtg ggctctcaaa tattcgagac tgagcctttt
    12721 ctccacgcga tagaggtaat gatggatttt gcgcgggaag cttgtgacgc aacgggcttt
    12781 atcatggaag aactggatat cggaggcggc ctaggagtac ggtacacagg cggtgatgat
    12841 cctctgagcg tggcggatct cgccgaggcg attgcgcccg cgttgctcaa ggcggctgaa
    12901 gagcggtgct tgcctcttcc cacattgctg atggaaccag gaagggccat cgtaggcgaa
    12961 gccgggacta ctctttacac ggtaaatgcg gtcaagcaca tagaaggcgt gcggacatat
    13021 gtaactgtag acggaggaat ggccgacaat ccccgggttg cactttacca ggcagaatac
    13081 gatgcagtcc tggcaaacaa ggccgatcgg cctcccacat gcgaggtgtc gattgcggga
    13141 aattgttgcg agtcaggcga catgttggca tgggatattg cgctaccgga tgtggaagtc
    13201 ggcgatatac tggctatatt ttgcaccggc gcttacaact actccatggc cagcaactat
    13261 aatcgacatc tccgtcccgc tatggcgctg gtgaaggatg gctggtcgga cttgatcgtg
    13321 gagcgcgaga catacgaaga tctcgtccgc aaggacgtgg tgccagatcg gctcaagtca
    13381 tcacataggt cggcggcagt gagcgcagag tcggctatgc tcgtctatcc tagggcttcg
    13441 ggggcgaacg cgtctgaata atgggagaat gttggcgagg ctcggccgat ccgggttgac
    13501 atgggggtta aggttttgaa cgtccgtgat gaacctgcca accggatctg tgaagtggag
    13561 gatgaatatg cattcctcgg atgcaacgca ttgcggggag acgggtatac agccgtcctt
    13621 tccgatatat gccccgacat gcgagattcg aaccatgtca ggcagatcaa tttgcctcgg
    13681 gcagcggatc ctgagggctt catcagcagg gcggaggagt tgttcaggag caaagggcgc
    13741 gagcattgca ggtttgatct ggactgccgt acacggcctc agtgccttta cacggcgctg
    13801 aggaagtctg gctatgttcc gcatccaaca gtcgttcagg tttatcgcgg ccatgatgca
    13861 gagggtgcgg gcggagtttt cagtggattg agtggcggcg aaagagacga caggcagtca
    13921 gattttcacc cttctgcgag cgggtccgaa gagtggagga acttgatgcg ggagcactat
    13981 gcagggtatg gccagtgcgg tgcttttgtg gagcgcgctg ttgatctcgc catccataga
    14041 ctggcagccg cttggggaga aggacgtgat taccgggttt tcctggcctt tgcaggcgat
    14101 gattcggacg cgtcaggggt gtgtgagatc ttccgccaca aaggagtggc gaagatagag
    14161 aggctgtacg tgcgaaagaa cagtcgcatg cagggcatag gcagcagtat gatcatgcat
    14221 gcagtggact atgctcttcg ggcaggcgat gatctggcat atctcgttgc ggactcattg
    14281 gattggccga ggcgtatgta tcgcaagctt ggttttgagg atctgatgga gattgtggaa
    14341 tggacgcgca tatggggcgg gagcgcggat gatgagggtt atgttacatg gtgagcgctt
    14401 cgcggcatgc tctgcacagg tgacgtagcc ccaagggaaa gtaacccgca tccggtgagg
    14461 acttggtccc aaactcccat agccttaact agccgtttca tgccgaatgc aagtttggcg
    14521 ggggtcgtgc cgaaacctgt ctgttttcga gccacgatcc ccgccctacg tactctggct
    14581 atcatatagc ctatatagag tgtagtcggt gccaacgata ctgatgttca tgcgccaagt
    14641 ctgttgaccg gccgggtgcg gaatgctagt attatggcac atgtcgaagg gagctgaatg
    14701 gtatgcgcga gaaggttgag gcccttttgg atgagataag gccgtcgctt caggcagacg
    14761 gcggggacgt ggaactcgtt gacgtgactg aggacggcat cgtaaaggtc aggctgaaag
    14821 gcgcttgcgc cgggtgtcct atgtcgactc tcaccatcaa aaatggtatt gagagggtgc
    14881 tgaagaacga gatacccgaa gtaaaagcgg ttcagtctgt ataggcttcg cacaatcgca
    14941 agctcagacc ttgaaggacg aggcggcggt ggatgtgccg tcgccttttg cgtggctaat
    15001 cgaagatgaa gagtcctagt gcttcatagg tccgcatgtc tacgattccc gtcactggaa
    15061 gccccttgtc tcgttgaagc tgcattatgg cgcgttcggt tccggggccg tataccccgt
    15121 caaagccagc gtgctcatag ccgagtttgg caaggcgttt ctgcacctcc agcacgtaac
    15181 ttcctttggc gcccggctcg atccggggac ggaatgaccc caaagtgccg aagtggccgg
    15241 cggttatgac taccggcgtc cctacgggaa ccattgagta gagttcttcg acatcgcggt
    15301 tgagcattct gatgcagccg ccagacttgc gttgtcccac agtccaaggc ttgtttgttc
    15361 cgtgtatccc gtatttcccc cacgggacgt taagtcccat ccaccttgtg ccgaatcccc
    15421 cggcccatgc cgccttctct atgactttga actgccctaa aggcgtgggc gtagatggct
    15481 cgccgacgcc gacccggtac tgcttgatgg gcaaaccgtc ctcgaggagt gtgatggtga
    15541 ggttggatgt atcgataagg agagcgcgcc agcctggcgg cagtcccttt ctggttgtgc
    15601 cctcttgttc ggtttggggt ttggccggcg aagtttttgc gatgggaatg atgtccccac
    15661 tcggttccaa tgaagctgcg acaatctgtt cgcatactcg gccaagggtc atccatacgg
    15721 acaggtcaac tatgccgtct tcagacaatc caacactggc ctgaaaagcc cgcacggctc
    15781 cttccgttgc aggtccgaag agcccgtcga catgtcctgc aggaaatccc gcaaccgcca
    15841 gcccttcctg taaatcgcgg acttgctttc cgaccattct gggctcgcac agccacagct
    15901 tgtgtagatc cccacattcg cagagcgttg ccgaacgagg ctccgattcc gattctggag
    15961 atgcgcctcc gccatcgtcg attggcacta cggctccggc ggctgactca tgatcctgcg
    16021 atagctttct gctgccgggc caaaccgccg attcttctgc gtttgcaggc tcgctcctga
    16081 acccattggg tgtttccgga gtttgcgggg agtatccttt ggagaataca acagcgcaga
    16141 tgatgcccgt aaggacaagg gctgccaagc gaagagggcc tcggccgtct ctttcagggc
    16201 ttctcgtcaa cgcaaccacc cactagaagt cgatgtgttt ccagcatagt ctttccagca
    16261 acatcatggt taacgcattc cagtaagcag gtatgcggaa aaaaggctag aatacatatt
    16321 caaatagtca acaatggccg gccaacatat gccgacacag ttgaaggggg cttatggatg
    16381 cggggagaga tgcgggcaat atccaagatg aggcccgagc caggcgcaga gctgataacg
    16441 gcgcctattc ctcaaccggg aagaggagaa gtactggtcc gggtgcgtgc aacagccata
    16501 tgcggcactg acgtccacat atatgagtgg gatccgtggt cgcaggcccg agttacaact
    16561 cccagaatct ttgggcatga gttctgtgga gatgttgtgg aagttggaga gggcgtaacc
    16621 aaggtcaaag aaggtcagtt cgtatccgtt gagacgcaca ctgcatgcgg caagtgcttt
    16681 tactgtctga ctgggctcgc tcacatctgt cagaatgcga agatcatggg catagaccgc
    16741 gatggatgtt ttgcagagta tgttgttgtt ccggagttca attgctggat atgggatatc
    16801 gatatatcgg acgaggtagc ggcgattcag gacccatttg gcaacgcagt ccatacgaca
    16861 cttgccactg atcttgtggg gaaaacagtc cttataacag gaatgggccc aatcggcttg
    16921 tgcgctatca gtgtcgccaa agccgccgga gctactgagg tgtacgtaac cgatgtgtct
    16981 gattacagac ttgacctggc ttgcaagctt ggggcagatc ttgcgatcaa cccgaagcgg
    17041 gaggatcccg tagcggctgt gcgcgaggcc accggagggg taggcgccga cgtgctgctg
    17101 gagatgtcgg gaagccccag cgctattgtt caggggtttg atgcccttcg aaaggcggga
    17161 ttcgcatcac ttctgggaat cccttctgag ccgatcaagt tggacctggc caatgcgatc
    17221 atattcaaaa gcgccactgt tcagggcata aatgggcgca ggatgtttga gacttggttc
    17281 caggcaaggt cgctgcttaa ggcgggcctc gatatcacgc cggtgataac ccacaagatg
    17341 tcgctatggg atgatctgga tgaaggaatg gagctcatga tgtccggcca gtgcggcaag
    17401 gtaatcctct atccgtagcc gcggggggcc ggggttttta agaattgggc ggaaattaac
    17461 agtgtcttga ggcgttggtt gacatagctc cgccatgccc taggcaacac tgaccgccgc
    17521 cgcgggaggg atttcatatg ccggaagtca tctattccga agagaagcta aaggcccaag
    17581 acggtgtcga actcttttgc cgggtgtgga agggcgtgag tttggagggc gaacctgctc
    17641 tgaaggcgcg ttccacaatt gtcatagcac acgggcttgc ggaccattcg ggcaggtatg
    17701 agcatgttgc aaagttcttt gcccaaaaag gatatgttgt ttacgcctat gaccaccggg
    17761 ggcagggcaa gtcagaaggc gtgcggggac atgtcaacaa attctcacaa ttctatgatg
    17821 atctttggtt tgtgatccgt agagccaagg gccgcaatcc cggaaagaaa tgcttcttgg
    17881 tggggcattc gatgggcggg ttgattgcct taggctatgc agccagacgt ccgaagacga
    17941 taaacggttt gatagtgact tcgccatgtt tggctttgcg aatgagggta tcgccgataa
    18001 agaaggggtt cggcacgttc atctcgtggc ttttcccgaa gatggctgtt gacacaggga
    18061 tagcggcaga ggggctttct catgactcga atgtagtgga agcctacaag aaggatccaa
    18121 ttcgctaccc caagatcaca accaagtttt atgtggagtt cgacaaggcc atgaagaatg
    18181 tgcagagctt ggcgaaatcg atcgtcactc cgtgcctttt catgcctggg ggagatgacc
    18241 cgatctgctc cacagaggct actatcaagt tctacgagaa tgtgcctgag actaccgata
    18301 agaagttgat agtctgggac gggctttatc atgagatcat gaacgagccg gaaaaggaca
    18361 aagtgctcga tacgatggcc caatggattg cagagcggga gggtaagtag tgcccgcgct
    18421 gagcagtgct gagcgccagc ggtacagcag gcagatacgc cttgccagag tgggcgaagt
    18481 tggtcagggg agaatccgag ctgccagagt cttgattgtg ggcgttggcg gcctgggttc
    18541 tcctattgct ctgtacctgg ccgcagcggg ggtgggcgta atcggcctcg ttgaccccga
    18601 cgtggtggag ctatcgaatc tgcagaggca gatagcccac accacctgcg acatcgggag
    18661 gcttaaggtt gattctgctg agcgaaggat cctggctcta aactcggagg tgcaggttga
    18721 gaagtgggct gtggcgctcg atgtcgataa cgcgggggat ttggtagggc ggtatgacat
    18781 agtggtggat gccttagatt ctaccggggc acgattggtg ctaaacgatg cgtgcgtggc
    18841 ggcggggaag cccctcgttc acggggcggt gctgggctgg accggggagg ccatgacgta
    18901 cgttccgggc tgcggagggc cttgttacag atgtgtgttt ggcggcgcgg tggtggaaga
    18961 gggggctcaa tgttgggtcg gggacgacga tgcctgcact gcgataagac atgatgacgg
    19021 agcgcatcat ggaaggtccg aggaggagag ccttggtcag ctggccggtg cgcaggcgaa
    19081 ggatgatcga ggtaatggcc ttgagaagct cggcccattt gacccgtctc gcgttggggt
    19141 gctggctaca gtccccgcgg tgataggcag cattgaggct actgaagtgt tgaaactgat
    19201 tacgggagcg ggagacctgc tggtagggcg gttgttgctc tgggatggac tcgagatgag
    19261 gtttgatgag gtaaaggttg cggctgaccc ggagtgcgga gcgtgcggta ttgacggctg
    19321 agtaggtctt gacaagtttt gtgcgattcc gaagtctgtg ctacctgcgg ttcaaaactg
    19381 ggccaattga tctggacaag atgctgcttg tgattgctca ggcatgtctc caaaagcgtt
    19441 cgagcgctcc taggagcagc tgagtcctta cgccggatag ccaacagtag cgcaaacgcc
    19501 gcatgccgtt tgaggctagg acaagaagtg tgcggcgatg gccaagtgct gccgggagaa
    19561 gaacgaacct ggctagattt tagtcacctc ccattgagac gaacgagcga accgccctca
    19621 tgatggaaga ttgggccgac atatggctcc atagaagcat gctctgagat gatgacgtgg
    19681 atgtgggcaa aaagacggtg ggtatcgaag gcggcacacg gtcctggttt gcacgggcgc
    19741 agcctgcggt gatatcatga gcccctgaag tgagcagacg agctgagggt ctgcttgatc
    19801 tttcctgtgc gctcagcctg ctcatcttct gtgcccaaat gccagcgtcc gatctttggc
    19861 cattggcgaa tgactagagc cgcaatgcag gctcatagct ccgaaatgct gatctcgccc
    19921 atggatgcgc ggactttgag ttctcctgtc ggcgtgccgt tgccgagaat tccggtggca
    19981 gacgacttct gattgtcgga gttcagacct gtaatcggca gcgagctatt tatctgtccg
    20041 gcggaaattg ctgcgtctat tcgcacacac gtagacctag gaacactgat cttgatggga
    20101 cctgcagagc tctcaatgta attgttcttt ccgagagttc caaggaagtc tatgggcccc
    20161 attgacgtaa tcaggctcaa cttgtttgca acgactgcgt cacgcacgtc tatcgcgcca
    20221 acggagacct cgatatccaa atcgcctctg gtggaatcgg tgttcacctt gcccgtatct
    20281 actttggcgt agatagcccc atcgatccca ttcagggtga tctcgcccgc agagagcgtg
    20341 gacctaacgt ctatccctgc tggaacctga attaccatgt tgattctgct ggacgagccc
    20401 gttgtagccg atgtgtcaac caggctgatt atctcgagaa catcatcgcg agtccgggct
    20461 tcgagcggca caagtgacag cttcgtctct gctttttcca atgtctcgcc gtaggcgatc
    20521 cgttcgccgt ctatagtgat gttgttgccg cctgtcttga tgactgtaat gtcgcctacg
    20581 ggtacctgta tagccacaca cttgatgccg gcggcgctga aagcctcctc aaactgtgct
    20641 tttgcaatga tgcctgtatc cgtattgatg taaggatcga acatggatga atacttatct
    20701 gcaagcaggt tgcttcctga cacccatggg tgggtggagc tcgctactac agccagcccg
    20761 ataagaacga ttataactat ccctcgccga gaaatcatct attatgtccc tcctatttat
    20821 gaagctctat ttgtcgatca ggcgtcgact tccaactcaa ggatcttgcc ggcgatcaag
    20881 atgagaatta cggtgcccat gatagtcggc cagaacggaa gcaacggtat gactttgcga
    20941 gccgccatcg gtcctagccc cgtgtataca atcagccccg ggttgggcac gaaggagaaa
    21001 gcgtacatcg cgtgcggtgc gaacgcaaag gtcaaatacc atgtgaggcc gtagacgatc
    21061 acagcgatca aacctgagat gcgtggcact atggttccta tcgtgaagga gaagagtccc
    21121 accgcaacga tgaacgcagt gccaaggatc gaacatacca ccatccatat gatgtcgagg
    21181 gttctctccc acggcgtcaa acgcaggata tcctctgttg ctgacatgat agtggagtag
    21241 ggtattgagg accgttgggt cacaaaccat gttccaactg ttgcaacgac ggtgatgagt
    21301 cctaggccga gaacggccac gagcaacttg gcccagagaa ttccggcgcc gctcactgga
    21361 tacgatagga gctgcagaga ggttcggtcc ctccactcat tgcgcattgt gtagtatccg
    21421 actaccaggg cggctatgcc cattcccaca aagggtatga atgacagccc gaaggcgagc
    21481 cctccgttga ttcttgccga ccatacgggc gccttgatcg caagccacgt atcgaatccg
    21541 atggtagctg ccaaggccag caaaatgacg gtccaggagc cgcgccattc ctttcccaca
    21601 agtgaccacc aacgcttcac ttcaacacac ctccgcgaag atttcattta aggatttgcc
    21661 ccgttcggct cttagctggt cggcttcgcc cgtcaagaca attcgtcctt cagagaggaa
    21721 gatcacgtct tcgaagagct tttccgattc aagaacttcg tgggtggata tgattatcgt
    21781 ctgctcgcct gcgcgatacc tgtcggctat agtctcgagt atcgcggtgc gcgaaggcgg
    21841 gtcgatgcct gagagtggtt cgtccattag caccagctct gcgcggcgag acattgcaag
    21901 gaccagtttc aacctgccgc gctgtccctt cgagagcgac gagatcttgt tgtcgggatc
    21961 cagtttcagc tctcgtacaa ggtccatggc gtctgcgagg acgaagtcgt cgaacataga
    22021 actttcaaag gcaattgcct gcctcaccgt catccacgag tagaagctgt ctatatccgg
    22081 gatgtatgcg gttattgcct tcgttgcaat ggaaatgtct tgcccgtgta cttgcacttt
    22141 tcccgaggtc gggcttgcaa gcccggcgca tatcctcagc aaggtagact tgccggatcc
    22201 attggggccc aaaaggccta cgatctttcc ccggtccacg ctgaggttta cgcctcgcag
    22261 ggcgtgggaa cggtaatatc ttttgtggac atcagtcagc tcaatagcgg gcatcactga
    22321 acacccccat catggtcatt tgtagtgctt ggaccgtttt gagcagctcc gttggagcta
    22381 ttggttccgg ttttggccag ttgctgtgag acgagcgaga ttatgtcgct gtcggagtag
    22441 cgcaaccgcc tcatctcttc aacgaaagac tccactgctg cagtggtcat ctctgatctg
    22501 acctccctcg ctatttccgg gctcgcggag acgaaagttc cttgacctct ttgagtttcc
    22561 accactccca tcctctcgag ctctgcatat gcgcgttgaa ccgtgttcgg attggtcttg
    22621 gcctgtttgg cgaagtcgcg caccgacagt agcttgtcgc cgggaagcag ggcgcctgtg
    22681 gccacatccc agagcacgct cttcattatc tgcgtgtaga taggtacaga ttcgtcgaag
    22741 ctgatcccca atcagatccc tcctttccga agcgcttcat ggcgctgtca tactgtacta
    22801 gtggactaat acagtgacac tataatatga tgccgttgga aggatgtcaa ggggttttga
    22861 ggactattct gggtggataa taaagggtgc gttgaacgat ggaactaggg cgtcgggctt
    22921 gccgagagcg tcatgcgcag ataactaagg tgttcgttgc gactatacat cccgcgccag
    22981 cgctagttcg attatttgca ttcccgaggt cgccgttgac aaccccacct ggggatgata
    23041 aaatcatgtt cgcaggaatt tagatatggc ggcgtagctc aggtggtcag agcatgcggt
    23101 tcatacccgc agtgtcactg gttcgagtcc agtcgccgct accatacgaa attacagcgc
    23161 catcagggtt tgcctggcgg cgctttttcg tttgcattgg cccgcttgtc gctgccgaga
    23221 acagtcctgt ttgatagtga aaaaatgcat aggttaaccg ccacgcctgg ggtcgacatt
    23281 cagggctcta cggggccgat ccatgttagg aaggggattg acgatgggat agctgttgtc
    23341 gagtggagtg ccaggtgcct gctgtgttca gagactgaga ccttgctatg gcaggcggct
    23401 agaagtggtg aagcgtttgg tggcagttgc cccgtcaacg tagtttagga cgaggcgagg
    23461 ggggtacacc gtgcctgcaa tggggatagt gatcggcgct tccccgtaga aatctccctt
    23521 gctgattagg ccgttgccat cgacgtccaa aaagccaacg agcacgtgat cgccggctgg
    23581 cgccgagaag atagtgaatg tgcggtcgcg cgtaccttgg acggaatcgg ttatccactt
    23641 tcctgcatta tccttgatgg cgaagactgc cctgtcgatg gtcgcgccga ctagagccgc
    23701 gtaagcgttg acgaggcctg caccgaattc gtggtcgcga ccatcggggc ctaggtccat
    23761 agaggtagcg cttatgagct ccgcgataga agccgggctc atacgaccgt gggtcgctat
    23821 taggagcgct gcgatgccgc ttacatgggg tgttgccatg gacgttccct ggctgaggaa
    23881 atatttgttg ggtgccggat ttgcgctact gtatccggtg ctcagcactg ggaaaagggc
    23941 ggtgccgccg ggcgcggcga ctgtgatttc cgggccataa ctggagtagc tagcaagttc
    24001 gttgttttgg ccagtagcgc tcacagctat tacctccgga aaggcagctg gatacaggac
    24061 tggtgcgccg ctatcgtttc cggctgccgc caccattgtg actccacgtg agaaggcgta
    24121 tcttactgcg tcttccatgg ctgagccggg atgattggcc aggccaagac tgaggttgat
    24181 cacgtcagct ccactattga ccgcccatat gattgcctct gccacgttgg acgagcttcc
    24241 ttttccatcg gggcccagca ctctaatggg catgatctgg gcgccccagc ttactcccgc
    24301 cacaccgacc ccgttgttcg ttgcagctgc gatggtgcct gatacgtggg tgccatggct
    24361 gtatttcttc gtggaattgg gccaatcagt gggatcagaa tccttgtcaa tgaagtccca
    24421 gcctgcgact atcttgcctt gcagatcttc atgttcgggg cgtattcctg tgtcgatcac
    24481 tgcaacgatg acggggcttc cttcgccggt ggctatgtcc catgcttccg ggaggctgat
    24541 tgcagggtaa tgccattggt acaagtacag ggggtcattg ggaactgccg cagcgtatac
    24601 agggtaatct gggtatgccc aatctacgcc atcgatggag tcgagggtcg ccgcttcgct
    24661 ttcgacgaga aatcttgcag tgcgttgcat aggacgtatg aggatgatgc ctgttagggg
    24721 cagttcatcg acgatctcgt atccggcttg gagtaggtta tgtgcgagtt cgtcgcggtc
    24781 tgtgccaggc cggactttca cctggatggt gtcggcttct gtttccggcg caggttgctg
    24841 gcctagcggg gccttttgga atgctgacag tgaacgcgaa gctgtctttg cgctgagaac
    24901 atggttcgac tggtcctcct ccgggtagta gttgtttgca acggttgcgc agcccctgac
    24961 agttgccagg cctgaggtgt tcgggtatag ttcgatctcg cagttgatac tgttgccggc
    25021 tggcacagta acatttacct gtcttggcct atagcctggg catgatgcgt taaggattac
    25081 tcttcctgca ggcacattgg acagggtgaa ggttccgttg ggcccggtgg cagcggacct
    25141 tttggggccg gcgtccacag tagctccacg taggggtgta tgtgacatgg catctttaac
    25201 ggtgccaatg atcgagccag tcttcggagg tgagggcctg ccgatgcagc cgctcaggaa
    25261 gatgcaggct aaggctgtga tgaggagata tgtgatgctg cgatgtgcgt gcaaagcaat
    25321 gccccctacg agtgttgcta aagggaaaga tcatgtcttg gagatattcg aggacgaggg
    25381 attggatcct ctaggagaga gcaggaaatg cttgggggat aggacgcgtc cgagttttta
    25441 ggctctcgtt agcagaattg gttctgttcc tgtggttaag gcatgaaata aggggagggt
    25501 gacagcaagg acgcttgcag cagttatgcg gttttgggac gaactatgct ggcacagatg
    25561 ttttcaacca tcaactggga cattggccaa agccttatgt gtgatggcaa aacttatgct
    25621 tgaaacccgt ggcggggcat agtataatgt aagctgcgcg ggggtcaaga aggctccagc
    25681 gtgtaactga atggatagat aatgtgtgcg ccactagctc agatggtaga gcagctgact
    25741 cttaatcagc gggtccgggg
//
LOCUS       DAOSYL010000031        25611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1586_length_25611_cov_7.715214, whole genome shotgun sequence.
ACCESSION   DAOSYL010000031 DAOSYL010000000
VERSION     DAOSYL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 25611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25611
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_25611_cov_7.715214"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLU15_06560"
     CDS             complement(<1..770)
                     /locus_tag="PLU15_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HOO30620.1"
                     /translation="MRHQSAYGSESQPGQVGFTYDANGHLASSTSINGVSTAYATDAQ
                     GRLKSITATRQQPFENILQLGYSYTSQGNVSRISGINGGSPFELNYGYDRINRLTFAQ
                     VPRPGGMANATYQYDGAGNRTVESWSDTGTVQYQYLPGNYLRTRGTTVYSWGPYGQLT
                     SKTQGQTRTDYAYSAQRLMNGVKVDGAALAKYEYDAMGRRIKTIQGETTTITLHSGND
                     IVYEVRTMPQAAGSNAGPNAIPGDPIIVDPIGPIIEDPP"
     assembly_gap    1636..1735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1736..2172)
                     /locus_tag="PLU15_06565"
     CDS             complement(<1736..2172)
                     /locus_tag="PLU15_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO30621.1"
                     /translation="MEAILTRRSIRKYTSEPVTDDEVKQLLRAAMHAPSAGNQRPWEF
                     IVIRDKETLNEITGIHKYAQMLKQAQVAIVVCGDVKKQKYDGFWVQDCSAATQNMLLA
                     ATALGLGAVWLGVHPVVERVEDLRKILGIPVNVVPLSIVSVGRP"
     gene            2550..3536
                     /locus_tag="PLU15_06570"
     CDS             2550..3536
                     /locus_tag="PLU15_06570"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOO30622.1"
                     /translation="MFGYTKPYGQHSLANAANVPQQGAQACRNLAAKETCSYKAHYCG
                     LCFALSRQYGPLWRACANFDSTLLLLLMSAQQPDAPTLTKAFCPLGAPRRYCVIDGDE
                     LFMKIGSAFTVAMVDLKSQDAIIDGARAARPARLLGRRAFARAERVLAENHFDASSLD
                     EIQKRQGKLESMAVENPLSVSFDDLAAPTGHGLAKVFEYTADLAGLRPNAEPLRRIGN
                     AAGKIIYAADCLEDLETDLARGRFNGLAACSMVVPGTLILTGEASAGLKYLLDRCRAE
                     IAYALRELETVRYRAIIENILLTSLPLRASEVLASRTESGHEPNHHKVDDIG"
     gene            3533..4006
                     /locus_tag="PLU15_06575"
     CDS             3533..4006
                     /locus_tag="PLU15_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30623.1"
                     /translation="MKIESLKIVIDSDELEAALKCIEMPQNVRIRGAHFEPDELVVDL
                     SVLAPIPLYPSFHVCVIQAAGPTVSFQVSTPLMTGLVDRFISNATTNLPDGVAYLGKG
                     IVSLDIPTLANGTVSSINIRGIQFEDGHATVAVDEIDIDISAITQLSSVNRPGSV"
     gene            complement(3984..4694)
                     /locus_tag="PLU15_06580"
     CDS             complement(3984..4694)
                     /locus_tag="PLU15_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30624.1"
                     /translation="MAKKNNGARPLAESAIAMALAGIMWHVFAPMPGIGPYFKYMSAS
                     LIAIVGSRHGRHWSTMTAVGLNALVGLAYGPKELLDSLLLVTPIGLMLPTGGPDGDEA
                     ADDRREFGLLNTETDKSKAEASFRRHDTWSAGKWWAMILLSYALLYPARVWALATLMG
                     AKVSDVVGSMAATSYHVFERFWALANISADTVVGAAVDWMRIILPVVGMGYGAMLYLV
                     NRPAYFGGLAILKRTRVG"
     gene            complement(4696..5703)
                     /locus_tag="PLU15_06585"
     CDS             complement(4696..5703)
                     /locus_tag="PLU15_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO30625.1"
                     /translation="MDTRALYQERLGPIVRRVEQALADCSDLDPEFEKMYWAAVGGGM
                     RFRPCLLQTAFVMCGGENEEIWIPAALAVELLHKASLIHDDLVDGDPLRRGKPSFCHV
                     YGAQRAVIMGDLLVARAYRAIDGLEDLVRGEAARRARQRFVAAHYDMCRGELLELMPW
                     RGAPTVDCAVRVISGKTASLMEQSLAIGAVLGGGNAQEIDALAKYGRFLGFIFQTVND
                     INNLTGFDSNTKGASLGDLEEQRVGFPMVGLAMQVGEERLAALSAQARRDAEGAKQAG
                     IEMSVLLEEGPVRQWLEGILEDLQQEAVRALSAFPESFERHALESICKDMFESWFWEP
                     D"
     gene            complement(5703..7235)
                     /locus_tag="PLU15_06590"
     CDS             complement(5703..7235)
                     /locus_tag="PLU15_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO30626.1"
                     /translation="MKAVGIDLGTTFSCLAVLNDYGKPEIVPNKEGERITPSVIMFDQ
                     ENQFTVGTIAKNSAVADPGNVVEFVKRQMGNADYVFVHGETEFRPEDLSALIIKKLVT
                     DAEVLLGTELPGAVITVPAYFDDARRQATMDAGEIAGLNVLAIVNEPTAAALAYGMSN
                     PDINQRIIVYDLGGGTFDVTILEVEEGKIRVVATDGDHMLGGKDFDDRIIMHCARKFE
                     EEHGIDLLEDPEALQDLRQRAEAAKKALSTRQSAKVAVSAQGKRHTVELSREQFEEMT
                     ADLLGRSELLLDTVLKSAELTWNDIDQTLLVGGMTRMPAVCELVRKASGKEPTVSINP
                     DEAVAHGAAIYAGIMLAGKGDPDIILTDAGRRLRDTKIEDVTSHSYGILLVDSATGQD
                     INKIMIPKNSPVPAERKETYYVHEDGQRIVEMVVLQGEDSDPANCTEIGEAVLELDGP
                     KPRNYPIEVVYKYDSNMRMDAVITDPQTGASKALQITERGRLSSAEVEEKREAMKDAE
                     VI"
     gene            complement(7264..7935)
                     /locus_tag="PLU15_06595"
     CDS             complement(7264..7935)
                     /locus_tag="PLU15_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30627.1"
                     /translation="MTTAEIHRIWADFDVSMGGRNGLIIHSSMTVRGYNPGAGREMTI
                     IYWFYYTDNSPVRGAIDEYADVAGNACTAEDFTPPYRDSTYDDFIVFMPYDAFGFDRA
                     GYFEAYCNLGIYYGDKLVARRKRVLMFALSSAGGQGQFEDRPREGRGRPDVLPPPLEE
                     RDGHEDVWSQLGIPRSASRNQKEQMLLEQYNTYRARVNHPDLEKRQEAERMMALIARA
                     RQELA"
     gene            complement(7957..9492)
                     /locus_tag="PLU15_06600"
     CDS             complement(7957..9492)
                     /locus_tag="PLU15_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO30628.1"
                     /translation="MGRAVGIDLGTTFSTLAVLNEYGKAEIVPNAEGERTTPSVVYFG
                     DDDSVVVGRVAKNAAVADPYNVVEFVKRQMGNADYMFLRDGREYGPEEISAFILKKLV
                     QDAEALLGEGITDAVITVPAYFDDVRRQATLNAAKIAGLNPLRIINEPTAAALAYGMG
                     NNASKQRILVYDLGGGTFDVTIMDVSHGEIRVVATDGDHMLGGNDFDDQIMILVNEAF
                     RKEHGVDLLEDLENQMDLRQRAESAKKTLSTLESAKISVSAAGKRLSHEITRSEFEEA
                     SGHLLQRTRLLVESVMASAKASWKDIDTILLVGGSTRMPMVREMLESMSGKAPNQAIN
                     PDEAVAIGAAIQAGIIMGQRGDASILKTTEGRRLANTVVTDVTSHSFGIILMDTMTNE
                     LYNQIMIAKNTPIPARIVDRFYTVSHGQEGVDFVVLQGEDRDPENCVAIGETKLLFER
                     PVHKGYPVEVTYEYDANMMIHAYMEDKKTGRRAELHISQRGRLTDSEVAEKKRLMDRM
                     RVD"
     gene            complement(9493..10050)
                     /gene="grpE"
                     /locus_tag="PLU15_06605"
     CDS             complement(9493..10050)
                     /gene="grpE"
                     /locus_tag="PLU15_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOO30629.1"
                     /translation="MDAGQGRMDDDTIGLADVQTPCIHEKLDVLIAMFEEKIEYDEAK
                     DKAFNKLYEELDMFKNDFVDRRMNEVYADLLLLYDNIKSYISRDDVQAPENEVARECL
                     EYVLEEVLEVLYRRGIERMACESDAFDPQCQRAVRMERANSPGEDGKVVKVMKDGFIK
                     DDRVLRHQMVAVARWDGDGHERVDD"
     gene            complement(10095..10529)
                     /locus_tag="PLU15_06610"
     CDS             complement(10095..10529)
                     /locus_tag="PLU15_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30630.1"
                     /translation="MVLNTSLAIGPVMWDNFNCDSASWRENTLRFTRTLSDHGLGQLV
                     YDLLMEAFKADQAGMYLHSHSFLATLEDLLVQEGELESFIYTMGGKARPADPGDDAPL
                     PIPGPSPGPKMTGGDGPCDNDELCEQCVAGFCVYSICQSCSG"
     gene            10910..11431
                     /locus_tag="PLU15_06615"
     CDS             10910..11431
                     /locus_tag="PLU15_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOO30631.1"
                     /translation="MFSENMLAELNKQYNYELYSGYLYMAMEAYFHGKGLSGFANFFK
                     VQTEEERAHARIFYEFIIRKGGTITLDSIEKPEADFDSPLDLFKKALAHEEFVTSRIN
                     KLVDIALEDKDHATNSFLQWFVDEQVEEEENFQGIVQKLELIGNDVHALLMLDAELGQ
                     RVFTVPAPLAQAQ"
     gene            complement(11587..12234)
                     /locus_tag="PLU15_06620"
     CDS             complement(11587..12234)
                     /locus_tag="PLU15_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30632.1"
                     /translation="MDTKRAFSAIAVASVLLLGVVGCFRQKASSNELRSYFPEKTGFT
                     WVYSGFAEYGHRMILDSITEVRSKGDVVHQISGMVEDMSGGESEQDFGFQLQYVFGGD
                     EVVERILHKGVVFPHRIDGLVMLKAPLEKGQKWSFESVNGNRVDAEITETGVDAEDRL
                     DYCKVEYTAPDPEMPDGLYHETRVFKKGIGVVSFTSTIMPDIEFSYTLTSVSSPD"
     gene            12551..13378
                     /locus_tag="PLU15_06625"
     CDS             12551..13378
                     /locus_tag="PLU15_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO30633.1"
                     /translation="MIPIRAYYSIGEISRLFGMSIQTLRHYDRIGLLRPAYVNHDNNY
                     RYYSHEQFLQIKLIKYLKTLGFSLAQIRDIVNQDLSSEVIIELLREQEEAVNNRIDQL
                     VALRQDMRAFEERLAGLAERTFGEAFLDSKPERKVIAYDYINKPNANLAVGMRKILAD
                     IEREHSVLKYEPGMLVSYTEFAASGRVEFKKIIVELDDELDYPHHNILRLPAGPHATI
                     IYEGATEEGQKYYGKIVEFISDHGLSPKGDFIHAIAVSYVNSNGTGVSLHEVSVLTE"
     gene            13450..14787
                     /locus_tag="PLU15_06630"
     CDS             13450..14787
                     /locus_tag="PLU15_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO30634.1"
                     /translation="MSKFLHYTVPSVMGCLVTSLYLVVDGVFIARGVGETAVAAVTLV
                     LPLTMAFVALSMIFSVGGGNLVSTSRGAGDQARAVNVFRESIYMLLSIGIIFSILGTL
                     FADEIAIMLGATGELVSPAAEYTKYYMLFTVPVLLAIALSTFIRHDGSPKLSMRSMLY
                     GAVTNIVLDYVFVFPLRMGLRGAAIASGLGQIVTVITCMKHFIERRGILRIGKASFEW
                     EDIKDIFVLGFPSFLTEVSYSVMMYLHNLAVISQVGEIGVTAYGIVNYINNISYMALF
                     GIGQGIQPMVSYYYGRGKFDKGRQQYIYGIKASLVMSVLFLVICLFVGRPLIMIFASS
                     EDVIDLGYTILTYLNLAYVLLGVNLTTQMYLQSMHMPRYANIICVLRGFVFVKIGLAL
                     LPKVFGDAGIWGTLLFAEGMSLITALVIVSASAEPEQYPSPIGNSQTDHASRP"
     gene            complement(14860..15990)
                     /locus_tag="PLU15_06635"
     CDS             complement(14860..15990)
                     /locus_tag="PLU15_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO30635.1"
                     /translation="MKGSIVMEIGGDFCFADIRCANGEPSYQNVLDGLSKGRPVSMLA
                     SGRTAYLNAWLNLKKGGSLVRRALLPSYLCYSMLSPFHRMGVETEHYRVFSDLSVDTD
                     DLMEKAFDDIQGTLVVALNYFGFPDSVDLLSALAEIRRRGGAVVYDATHSALCERPWP
                     HTTFEPNLCIMSLRKSLPVVDGAVCVWLDGSEPAFCSNEAQLSGFHSARALAMMMKSA
                     YVADGFGNRRDYVLLFAQAEAALDSSFSASAAMSPVSRSILKRVGIEEIRRVRRRNFR
                     RLLEELATCGGVHALISELPEDAVPLGFPVVVEDRDLLWRHLEHYGIHADIHWELPPE
                     VPEEEYPESAWLARRILTLPCDQRYSEEDMARMARVLKEYRP"
     gene            16450..17313
                     /locus_tag="PLU15_06640"
     CDS             16450..17313
                     /locus_tag="PLU15_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO30636.1"
                     /translation="MQIVTDYAADISPEQLEGLEVHYLPLMITVDGKTYRSGVDITPD
                     QFYELQATAVEIPTTSQPSPGEFADLYRSLAQTDPDILSIHISSGLSGTVNVARIGAK
                     MVPEADVEVYDTKTLSAAQGWHVEMAAKAVRAGWEKSRIVAMLKQVTEASETLFTLST
                     LKYLIHGGRISHLKGLVASLLNIKPIIGVEKAGGTYVTRGQARTMTRAIDALADIVSS
                     MYPPETPMRAQILHSQNPTGVSRLQDKLDKVFVCNWLPTGPIAPVLGAHTGPGLVGIA
                     IAPEALFPQMP"
     gene            17366..17962
                     /locus_tag="PLU15_06645"
     CDS             17366..17962
                     /locus_tag="PLU15_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30637.1"
                     /translation="MKLRDLALRILMMISGLSLIGLGIAMFVKAGLGADPITVLCQGI
                     SITANISLGRALQLNGLALFVVLLIIGREYIGLGTFAGSFVTGAMADVFSAAMRSPTA
                     PWQSWPILIASIAVISSGVAIYLSAELGDGPLEGVMMLISDRLGAPIAWVKIAMDIAA
                     TVAGIAMGAVPGVGTIASVLLTGPIIQTMLQVIKKSMA"
     gene            complement(18002..18808)
                     /locus_tag="PLU15_06650"
     CDS             complement(18002..18808)
                     /locus_tag="PLU15_06650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO30638.1"
                     /translation="MIALRIGVNQIFTPNGDIGRFVRGTCGHIVDRNDDGSETTVGII
                     AGLWFQIDDWIIVGKPCDYRDMFSQFSPEVSLAGDLLVPEPPEIRRDVTDSDRHDIIL
                     LECVFVEKPYRNCGIGTAALEGFLRYARSRAGVVAIEPFPMEQGDDGVIRAALKSSVP
                     VEHKPAGPGSECELLPISDGGNCSTLWPRFLRRLGFEPIPEECCHHFAVDPSACSPLA
                     RQRAADGNPCPGLMTVDDIAVEVVYSPVQLPVPYEGMKAVVTIDAPDVAW"
     gene            complement(18984..21275)
                     /locus_tag="PLU15_06655"
     CDS             complement(18984..21275)
                     /locus_tag="PLU15_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOO30639.1"
                     /translation="MEVESLATEEQEKDGLRATAEDMEGQGTPNEAEIAAQMWAGIAT
                     KISGELGIARSKVDSVIRLLDEGNTIPFLARYRKEQTGGLDEEAIRSIDERLDYLRNL
                     ETRKLEVMRLIDEQGALTPEIRQLLETAETLQRVDDIYRPYRPKRRTRASMARERGLG
                     PLAEWLLLRPETGDPLERAAEYVNLEAGVASAEEALAGARDIVAEILADDPEVRAWAR
                     QWTAGHGEISTEATDSEARTTYEQYYDYCEPVNRIPPHRVLAINRGEREKVLRVRITL
                     EAEPIVAYLKRRLMREGPKSSPADAELALVAKDAYDRLLAPAVERDVRNALTEEAEQQ
                     AIRVFGANLKALLLTPPIRGKVVLGLDPAYRTGCKIAVVDATGKLLETTVVYPTPPQK
                     RIEEAEQVLLKIIDEHGVDLIAIGNGTASREAEAFVAGLIPKAERSISYVVVNEAGAS
                     VYSASKVAREEFPDLDVSERSAVSIARRLQDPLAELVKIEPRSIGVGQYQHDVSGKEL
                     STTLNGVVESAVNRVGVDLNTASVPLLSYVAGISPSVAANIVAKRESEGPFGEREELL
                     SVSRLGPKTFEQCAGFLRVPGGRYPLDNTAIHPESYPVAEHLLAELGFAVDDVAERDR
                     TKLCQRLSALDAQDWAERLDVGLPTLEDIIEALQRPGRDPRDEFPQPILRKDVLTMDD
                     LRPGMMLEGTVRNVVDFGAFVDIGVQQDGLVHISELSNRYVRHPLDVVSIGDIVKVRV
                     LSVDIERGRIALSMRDIGEEKLG"
     gene            complement(21555..22808)
                     /locus_tag="PLU15_06660"
     CDS             complement(21555..22808)
                     /locus_tag="PLU15_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO30640.1"
                     /translation="MTLRKQHCRFLLLLLLAIIIAWPQAALATSAAGSEQQEQMTVVV
                     CVIDGCPNLSLGCLPETAYLRQLCADERCQYKSIEAVFPSSTAAGHAAIFTGTYPERN
                     GVTGKEYLDDDGELLRFKDPAVLEEPTLFHVARSAGLGTAMISAKANLCKMLSEATDF
                     AIWPESVPDWVIETVGPPPDESTQYEDFAGWHIQLDRWVLRTVEAYLEQNDMPVFVGI
                     NVGSVDKCGHRFGPLPAAETAECLCSISEGLEELAATLEATRPGRWSLIITADHGMTR
                     VDTGIAARDIVGDITDEDVPITLDGGVLYVWAEDNRGKIADALRQTEGVAEVIEVEDV
                     ARRAELHIEHPRTPPLIAVTDRGCMFIEALLFMDYTLGSHGTTLDTDVDVPLIVVGRN
                     ADSVDIENVSSITDIASIARELLGI"
     gene            complement(22805..23320)
                     /locus_tag="PLU15_06665"
     CDS             complement(22805..23320)
                     /locus_tag="PLU15_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30641.1"
                     /translation="MRKYRLAVVFCLTFVILGSMCFASPAKPSGKVVLTVKGPIANTN
                     AKGACEFDLEMLEGLGLTEYQVKDPWLGKKTYAGVNIAKILGYVGAPKNVSEVVVIAK
                     DGKEVIISAADVAKYEIMVATKDGGKAIGSGLGGPVKLVFPYDDNPGLDKQYPKESWN
                     WFVTTIEVRTK"
     gene            complement(23401..24582)
                     /locus_tag="PLU15_06670"
     CDS             complement(23401..24582)
                     /locus_tag="PLU15_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO30642.1"
                     /translation="MESQASRTPKGIQLIAAGMLLWGFGYGLHSYMFPVYLRNIGCTP
                     QEVGFVYSISMAVMAASSIPGGMLADKYDRKRVILLSWIMGVPSVILYYFAKDWRLAA
                     LGIALYSGSMLGYPALNAYAGACSPEGESGRTFGLISAGFSGGMIASPILSAYLSSIW
                     PIENLFLLSFAFFAAACCVIAFLPPEPESESTASVSTYASLLADRSFMKFVFCYSLCA
                     FSFYMVQQIIPQYLSDVRHTNISIVSLLGSIMSLGQTMITVVVGRMADNRGTLVAVGA
                     NVIVFVISMLCFIAISGQVAVVICLFLLGGFMAGHGVAFAGVGEVLGDGADGKAFALF
                     NLATWGASIVGPYLGGFFYSTSENTPFYIAALLLVVGGTMLIRQGSTIHVVGEVRKGQ
                     G"
     gene            complement(24598..>25611)
                     /locus_tag="PLU15_06675"
     CDS             complement(24598..>25611)
                     /locus_tag="PLU15_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOO30643.1"
                     /translation="YVGLKLTGETCIDSSAASCMRWHSDDTGDITSSAPGARLFPRVV
                     DPWEAIGEVTREAADETGLPSGIPVAAGSIDAFVEALGAGVTSPGLICDSTGTSTCVS
                     AVAPDGCSLQTVKHVIPGERLIVSPISYSGGCLAWAMSVLYPEVYSSKLDWRDTVAEA
                     LDCSESGASGLVFLPYFVGKRSPVAVPRATGAFLGLRPEHDSCHMIRAVLEGCAYAMR
                     QSMEAWQDSMKAVDVRAVGNGARHRGWLQMKADVLNLPVASMKVKEGTLLGAAILAGT
                     AANVFSSIKEAVQVVVLTDAVFTPEGVRGDGYDRGYAAFCRAESALQEFWNSAEPAWP
                     AQH"
BASE COUNT         5888 a         7668 c         6136 g         5819 t
ORIGIN      
        1 ggcggatcct caattatcgg accaatcgga tcgactatta tcgggtcgcc cgggattgcg
       61 tttggcccag cgttcgaacc cgcggcctgc ggcattgtgc gaacctcata cactatgtcg
      121 tttccagagt gcaaggttat ggtggtcgtt tcgccctgga tcgtctttat cctgcggccc
      181 atggcgtcgt actcgtattt cgccaaagcc gcgccgtcca ccttcacccc gttcatcagg
      241 cgctgagcgc tgtacgcata atccgttctt gtctggcctt gcgtctttga tgtcaactgc
      301 ccgtagggcc cccaggagta gactgtcgtc ccccgggtcc tcaggtagtt tccaggcaag
      361 tactggtact gcactgtgcc agtgtccgac cagctttcaa ccgttcgatt gcctgccccg
      421 tcatactggt aggttgcgtt ggccattccc ccgggcctgg gaacctgggc aaacgtgagc
      481 ctgttgatcc ggtcgtaccc gtagttcagc tcaaacggag agcccccatt gatcccagat
      541 atgcgggata cattcccttg ggacgtgtac gagtagccaa gttgcagtat gttttcaaac
      601 ggctgttgcc ttgttgcggt tatggacttc agcctgccct gggcatcggt cgcataagcg
      661 gtgctcaccc cgttgatcga agtagagctt gcaagatggc cgttggcatc gtatgtaaac
      721 cccacctgcc cgggctggga ctcagacccg taagcagatt gatgtcgcat gaacacaaac
      781 ggtcatgcag catagtgaac aaaggcatgc aagcaggatg gctgtaatgc gccgacgggt
      841 ggacggcgct ctcacgggct cgaaatggct ggtcgcgaag ttgccgaaga acgccttttt
      901 catatcttct gcctcgcgcc gaaagccaca cgcttgtttc gttggaatat agaagacgat
      961 ggccctaggt ccgatttgtg gctggattgt atggtaacta tgtcattcgc ggtgttttac
     1021 ctaatccttt cgcaccgttc tgcttcttag tcataaacgc accgaattgg cgcatcgaac
     1081 acacgctctc tgtaattctt aggttctcac taactgcgca catcttccga caggttggag
     1141 caggtctccc agtcagtttg ccaagccgcc acatcacgtg aacgactgat ctcaacatcg
     1201 aatgtcatct tatcgcgcaa agcgcccata gagcttgctt gctacgcatg aggcaacagt
     1261 cttcacgggc agcactcgct aacgggacaa cagcaagcag cctgaggaca cacagcaatg
     1321 ctcggcggcg cttggcgaca ctggcacggc cccgttccgc ctgtcttgac tagacaaagt
     1381 ggttgccaat aagccattat gaacttgccc gaccgcgacg accgcaattg tgcctggcaa
     1441 gaacgaggcc gaaacctgcc ggcctatcga tagaggcaaa accagatggc tgagaagata
     1501 agtgagaaaa ccaagaagat gcttgttcac atatacgctc tattcctggc gttaggagac
     1561 aggcgagttc cctggcacac caaagcagtg gcagcaataa ccgtgggata cgctttctcc
     1621 ccaatagact tgatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtcg
     1741 gcccaccgac acgatcgaca ggggaaccac attgacggga atgccgagga tcttcctcag
     1801 atcctccacc cgctccacaa ctggatgaac tccaagccag accgcgccca atcccaatgc
     1861 cgttgccgcc agcagcatgt tctgcgtggc agctgaacag tcctgcaccc agaatccatc
     1921 atacttctgc tttttcacat ctccgcacac gacaatggca acctgagctt gcttgagcat
     1981 ctgcgcgtac ttatgtatcc ccgtaatctc attgagcgtc tctttgtccc tgatcactat
     2041 gaactcccac ggtctctggt tgccagctga cggggcatgc atcgccgccc tcagtaactg
     2101 cttaacttcg tcatcggtta caggctcgga agtatacttg cgaatgctgc gcctggttaa
     2161 tatagcctcc atggcatcca cttgctgact ccccccaagt atgagatgac atctcgttat
     2221 tggccgactc gacacagctt aagccgcgta agtcagataa atactgccga attcgctgtg
     2281 ttcacgtgat aggctcctgt atcgtccccc tcccatgttc actcaagtcg cgcgacatgg
     2341 gttctcccga ccagtgccac actctgccca tacgaaaacc cgcatggcat tgccgccatc
     2401 gccctcgcga atctatggtt caagcaggat atcgcagcct ccaaggcgaa gaacaggcgc
     2461 ttggaagcaa gtagtgcctt aacccgcgca acgagcgccg ggataatcag tcacagccaa
     2521 cacctccgaa acagcgggag gcggaccaaa tgttcggata caccaaacct tacggccaac
     2581 acagcctggc caatgcggcg aatgtgcctc aacaaggggc ccaggcatgc cgaaatttgg
     2641 ctgctaaaga aacttgcagc tacaaagccc attactgcgg actctgtttc gctctttcgc
     2701 gccaatatgg cccgctttgg cgagcctgcg ccaatttcga ctccacgctc ctgctcctgc
     2761 tcatgtcggc acagcagccg gacgcgccca cgctcacaaa agccttttgt ccattgggag
     2821 cacccaggcg gtattgcgtc atagacggcg atgaactctt catgaaaata ggttccgctt
     2881 tcacagtcgc catggtagac ttaaaaagtc aggatgcgat catagacggc gccagggccg
     2941 caagaccggc acggcttttg ggcaggcgcg ccttcgcccg agccgagaga gtccttgccg
     3001 aaaaccactt cgacgcatca tctcttgacg agatacaaaa gcgccaagga aaactggaat
     3061 cgatggctgt cgagaatccc ctgagcgttt cctttgacga tctggctgcg ccgaccgggc
     3121 acgggctcgc caaggtcttc gaatatacgg ccgacctcgc cgggctccgc ccgaacgccg
     3181 agccgcttcg gagaataggc aatgctgcag gcaagatcat ctatgccgcc gactgccttg
     3241 aagacctgga aaccgacctt gcccgggggc gcttcaacgg cttggccgca tgttccatgg
     3301 ttgttccggg cactctgatc ctaacaggcg aggcatccgc aggactcaag tacctactag
     3361 accgatgccg ggccgagata gcatacgctc ttcgggagct agagaccgtg cgctaccgcg
     3421 ccataatcga aaacatactt cttacatcgc ttccgctgcg cgcatccgaa gttttggcgt
     3481 cgcggacgga atccgggcac gaaccaaacc atcacaaggt ggatgatata ggatgaaaat
     3541 cgaatcattg aagatagtca tagacagcga cgagctcgag gccgccctca aatgcattga
     3601 gatgccgcag aacgtgagga tacgtggcgc tcacttcgaa cctgacgaac tcgtagtcga
     3661 tctctctgtg ctggcgccta tccccctata cccctcattc cacgtctgtg tgattcaggc
     3721 cgctggccca accgtctcct ttcaggtctc caccccgctc atgacagggc tggttgatcg
     3781 tttcatatca aatgcaacaa cgaacctccc tgatggcgta gcgtaccttg gcaaaggcat
     3841 agtaagcctc gacataccca cgctggcaaa tggcaccgtc agctcgatca acataagagg
     3901 tatccagttt gaagatggcc atgccactgt cgcagtggat gagatcgaca tcgacatctc
     3961 agccatcact cagctgagtt cggtcaaccg acccgggtcc gtttgaggat cgcgaggccc
     4021 ccaaagtagg ccggccggtt caccagatac agcattgcgc catagcccat gccgactaca
     4081 ggcaggataa tccgcatcca gtccacggcc gctccgacaa ctgtatcggc tgatatattc
     4141 gccaatgccc aaaacctctc gaaaacatgg tagctagttg ctgccatcga ccctacgaca
     4201 tcggatacct tagcgcccat caacgtcgcc agcgcccata cgcgggcggg gtatagaagg
     4261 gcatagctta agagaatcat cgcccaccat ttgccggcac tccaggtatc gtgcctcctg
     4321 aaagaggcct cggccttgct tttgtcagtt tcagtattca ggaggccgaa ctcacgtcta
     4381 tcatcagcag cctcgtcccc atcaggcccg cccgtcggca acatcaaccc gatcggcgtg
     4441 acgaggagca aggagtccaa caactctttc gggccatacg ccaggccgac cagagcattg
     4501 agacccacag ccgtcatcgt cgaccagtgc cttccgtgcc tcgaaccaac aatggcaata
     4561 aggctcgcgc tcatgtactt gaaatatggc ccgatacccg gcatgggcgc aaacacgtgc
     4621 cacatgatcc ctgctaaggc catggcaata gctgattccg cgagcggcct cgccccattg
     4681 tttttctttg ccacctcaat caggctccca aaaccacgat tcgaacatat ctttgcagat
     4741 ggactcgaga gcgtgcctct cgaaactctc ggggaatgcc gacaatgccc tgacagcctc
     4801 ctgctggaga tcttccaaaa tgccctcgag ccactggcga acaggccctt cctctaagag
     4861 cactgacatc tcgatcccag cttgcttcgc cccttccgcg tctcgccgcg cctgggccga
     4921 aagcgccgcc agccgttcct cccctacttg catcgcaagg ccgaccatgg ggaatccgac
     4981 tctctgttcc tcgaggtcgc ccagtgaagc gccttttgtg tttgaatcga accctgtgag
     5041 attgttgatg tcatttaccg tctgaaaaat gaacccaaga aaccttccat acttcgcaag
     5101 cgcgtcgatc tcctgcgcat tgcctcctcc aagaaccgca cctattgcca atgactgttc
     5161 catgagggat gcggtctttc ccgaaatcac cctcacagcg caatcaacgg tcggggctcc
     5221 gcgccaaggc ataagttcaa gcagctcacc tcggcacatg tcgtaatggg cggcgacaaa
     5281 ccgctgcctc gccctccgag cagcctcccc acgcacgaga tcttccagac catctatagc
     5341 ccgataagcc ctggctacga ggaggtctcc catgataacg gctctttgag ccccatacac
     5401 atggcaaaac gacggcttcc ctcgcctcag aggatcccca tcaacgaggt cgtcatggat
     5461 taacgaggct ttgtggagca actccacggc cagggcagca ggaatccaaa tctcttcgtt
     5521 ctctcccccg cacatcacga acgcggtctg cagcaggcaa ggacgaaacc tcattccgcc
     5581 gcctacagca gcccagtaca ttttctcgaa ttcgggatcc aggtcactgc aatccgcaag
     5641 cgcctgctca accctgcgca caatgggacc gagccgctcc tgataaagcg cacgcgtatc
     5701 catcagatta cctctgcgtc cttcatggct tcacgttttt cctcaacttc agcactggaa
     5761 agccttcccc tctcagttat ctgcaacgct ttcgaggccc ctgtctgagg atctgtaata
     5821 acagcatcca ttctcatgtt cgaatcatac ttatagacga cttctatcgg atagttgcgc
     5881 ggcttgggcc catctaactc cagcacagcc tcgcctatct cggtgcagtt ggcaggatcg
     5941 ctgtcctcgc cctgcagaac caccatctcc acaatccgct gcccgtcctc gtgaacataa
     6001 taggtctcct tccgttcggc cggaacgggc gagttcttgg ggatcattat cttgttaata
     6061 tcctggcctg tcgcagaatc gaccagaagt atgccatagc tatggcttgt cacgtcctct
     6121 attttggtat cgcgaagcct acgcccggcg tcggtcagga ttatgtccgg atctcccttg
     6181 cccgcaagca ttattccagc atagatagcg gccccgtggg cgactgcctc atccgggttg
     6241 atgctgaccg tgggttcttt tcccgaagcc ttcctaacaa gttcgcacac cgcaggcatc
     6301 ctggtcatcc cgcccacaag caacgtctgg tcgatgtcgt tccaggtcaa ctctgccgat
     6361 ttgaggaccg tatcgagcaa tagctctgac ctgcccaaaa ggtctgcggt catctcctca
     6421 aattgctccc tcgaaagctc gacggtgtgc cgtttcccct gggcgctcac ggctaccttg
     6481 gcagactggc gagtcgacag cgccttcttg gccgcctccg ctctttggcg caggtcctgc
     6541 agagcctctg ggtcctcgag aaggtcgatc ccgtgctcct cctcgaactt gcgagcgcaa
     6601 tgcattatga tgcgatcgtc aaaatccttg cccccaagca tgtggtcgcc gtcggtcgca
     6661 acaaccctga tcttgccttc ttcaacctcc aggatcgtta cgtcgaaagt cccgcccccg
     6721 aggtcataca cgatgatgcg ctggttgatg tcggggttgc tcattccgta ggcgagcgcg
     6781 gccgcagtgg gctcgttcac gatcgcaagc acattgagcc cggcaatctc ccccgcatcc
     6841 atcgtggcct gtcttcgcgc atcatcgaag taggccggga ccgtgattac tgctccggga
     6901 agttccgtgc cgagcaagac ttctgcatcc gtcaccagtt tcttgatgat tagcgctgaa
     6961 aggtcttccg ggcggaactc ggtttcgccg tgaacaaata catagtctgc atttcccatc
     7021 tgcctcttca cgaactcgac tacattgcca gggtcggcaa cagcgctgtt ttttgcgatg
     7081 gtgcccactg tgaactggtt ttcttgatcg aacatgatca ccgagggggt gatgcgctct
     7141 ccctctttgt tcggcacaat ctcgggtttg ccgtaatcat tgagcacggc gagacacgaa
     7201 aaggtggtcc cgaggtctat acctactgcc ttcataaatc gtcactcctc cacaccaaac
     7261 aactcatgcc agttcctgtc tggccctggc tatcagcgcc atcatccgtt cagcttcctg
     7321 cctcttctca agatcgggat ggttgactct ggcccgatag gtgttgtact gttcgagcag
     7381 catctgctcc ttctgattgc ggctggcaga cctgggaatg cccagttggc tccatacatc
     7441 ttcgtgccca tcgcgctcct ctagcggagg aggaagcacg tcaggcctcc cgcgcccctc
     7501 ccgggggcgg tcctcgaact ggccctgacc tcctgcactc gatagcgcga acatcaacac
     7561 cctttttctc ctggccacaa gcttgtcccc atagtagatc cccaggttgc agtaggcctc
     7621 aaagtagcct gccctatcga atccaaatgc gtcatagggc atgaaaacga tgaaatcatc
     7681 gtatgtgcta tcccgataag gaggggtgaa atcctcggct gtgcacgcat ttcctgcaac
     7741 atctgcatat tcatcgattg ctccgcgcac gggggaattg tctgtatagt aaaaccaata
     7801 gattatggtc atctcacgac ctgcgccggg attgtatcct ctcactgtca ttgaagaatg
     7861 aataataagt ccgtttctgc cgcccataga tacgtcaaaa tccgcccaaa tcctatgtat
     7921 ttctgcagtt gtcatttccc gctctcccgc tcgcggctaa tccactcgca ttctgtccat
     7981 gagccgcttc ttttctgcca cttcagaatc tgtcagcctg cccctttggg atatgtgtag
     8041 ttcggcgcgt cttcctgtct tcttgtcttc catataggca tgtatcatca tgttggcatc
     8101 gtactcatat gtcacttcca ccgggtatcc tttgtgcaca ggcctctcga aaaggagctt
     8161 agtctcgcct atggccacac agttttcggg atctctgtcc tctccctgaa ggacgacaaa
     8221 atccacgcct tcctgcccat gggagaccgt gtagaagcgg tcgacaatcc gcgccgggat
     8281 aggagtgttt ttggctatca ttatctggtt atacagttca tttgtcatcg tatccatcag
     8341 tatgatgccg aacgaatgag acgtcacatc ggtgaccacg gtatttgcaa gcctgcgtcc
     8401 ctccgtagtc ttgaggatac ttgcatctcc acgctgcccc atgatgatcc ccgcctgaat
     8461 cgcagcacct attgcaacgg cctcgtcagg gtttatggcc tggtttggcg ccttgcccga
     8521 catcgactcg agcatctcac ggaccatggg catcctcgtg gaaccaccga caaggagtat
     8581 ggtgtctata tctttccatg aggctttggc gcttgccatg acagactcca ccaagagcct
     8641 cgtccgttgg agcaagtggc cggaagcctc ttcaaactcg ctccgcgtta tctcatgcga
     8701 cagacgcttg cctgcagcac tcacgctgat cttcgcagat tcaagagtcg atagagtttt
     8761 cttggcagac tcggcgcgct gcctcaggtc catctggttt tcaagatcct ccagaagatc
     8821 aactccgtgt tctttcctga aagcttcgtt gacaaggatc atgatctggt catcgaaatc
     8881 gttcccgccc aacatgtgat cgccatcggt ggccaccacc cttatctcac cgtgcgagac
     8941 atccatgatg gtgacgtcga aggttccccc gcccagatcg taaacgagga ttctctgttt
     9001 ggaagcgtta ttgcccatcc cataggccag tgccgccgcg gtaggctcgt ttattatcct
     9061 cagtggattg agccctgcga tctttgccgc gttgagcgtc gcttgccttc tcacatcgtc
     9121 gaaataggct ggaaccgtga tcacagcatc ggtgatgccc tcgccgagga gggcctcagc
     9181 atcctgaacg agcttcttca agatgaaggc cgagatctcc tcaggcccgt actcccgccc
     9241 atcacggaga aacatatagt ctgcattccc catctgacgt ttgacaaact ccacgacatt
     9301 gtacggatct gcaacagcgg catttttcgc caccctaccg acgacgacag aatcatcatc
     9361 gccgaaatag acaacggaag gcgtcgtccg ttcgccttct gcatttggca caatctctgc
     9421 ctttccatat tcattgagga ccgccaaagt ggagaaagtt gttccaaggt caatccctac
     9481 cgcccggccc atctagtcat ccaccctttc gtggccatcc ccgtcccagc gggcaaccgc
     9541 aaccatctga tggcgcaaaa ccctgtcatc cttgatgaag ccgtccttca tcactttgac
     9601 aaccttcccg tcttctccag ggctgttcgc gcgttccatc cttacagctc gctggcactg
     9661 aggatcaaag gcgtccgatt cgcaagccat gcgctctatg ccgcgccgat agaggacttc
     9721 gaggacctct tcgaggacat attcaaggca ctcccgggca acttcgtttt cgggtgcttg
     9781 gacatcatcg cgcgagatgt agctcttgat attgtcgtat aacaaaagca ggtcggcata
     9841 gacctcattc atgcgcctat cgacgaaatc attcttgaac atgtcaagct cctcatagag
     9901 cttgttaaaa gccttgtctt tagcctcatc atactcgatc ttctcctcga acatggcgat
     9961 gagaacatcg agtttctcat gaatgcaagg cgtctgcaca tccgcaagac caatcgtatc
    10021 atcgtccatt ctgccttggc cagcgtccac tcagaattct cctttcccca tgagttgcac
    10081 atgcttttgc gcccctatcc cgaacaggat tggcagatcg aatacacgca gaatccggca
    10141 acgcactgct cacacagttc atcattatcg cagggtccat cgcctcctgt cattttcgga
    10201 ccaggactag gcccagggat aggaagcggc gcatcatctc cgggatcagc cggccgtgcc
    10261 ttgcccccca tggtatagat aaaggactcg agctctcctt cctggacaag caggtcttct
    10321 agggtagcga gaaacgagtg gctatgcaga tacatgcccg cttgatcggc tttgaaggct
    10381 tccatgagca gatcataaac gagctgcccc agtccatggt cggaaagggt gcgggtgaaa
    10441 cgtaacgtgt tttcacgcca cgaggcggaa tcgcaattaa aattgtccca cattacaggg
    10501 cctatcgcca acgacgtgtt cagaaccata actccatcct ccgtccgcct cccaagggca
    10561 cttcacagat ttcataacta gtttatggtt tctacaacca gctatggaat ccttcactat
    10621 ttgcgtccaa aggcaaagca gaacatgacg gacgcaggca gagttgccca taaatcagct
    10681 gcctgttcaa ccaggatggg tccccgcgct cttcactcat gcagcttgat cctcgacagg
    10741 caaggtcttg gcaatactcc cttccgccag acccgctaac ctacgttcat gctatggctg
    10801 cagaagcaaa caccgcttga attttctcgc gctggcagtg tacacttaga attgctagtc
    10861 cacaaattgc ctgagcaacc acgtgctcag gatacgagga ggcatgatca tgttttccga
    10921 aaacatgctt gcagagctta acaagcagta taactatgaa ctatactcag gctatcttta
    10981 tatggccatg gaggcctact tccacggcaa gggcctatca ggtttcgcca acttcttcaa
    11041 ggtacaaact gaagaagaac gggcccacgc gcgcatcttc tacgagttca tcattcgcaa
    11101 aggtgggacc attacccttg attccatcga aaagcccgag gctgacttcg acagtccgct
    11161 cgatctgttc aagaaagccc tagcgcacga ggagtttgtc acctccagaa tcaacaagct
    11221 ggtagatatc gcccttgagg acaaagacca cgcaaccaac agctttctcc agtggtttgt
    11281 ggatgaacaa gtagaagagg aagagaactt ccaaggcata gtccaaaagc tcgagctaat
    11341 cggcaacgac gtgcacgccc ttctgatgct cgatgccgaa cttgggcaga gggtcttcac
    11401 agtgcccgct cctctggccc aggcacagta agggcccaag gttttatcag ctgtccatca
    11461 attgcgggca ttcaaagcta gaatgagttg aggcacagcc caaagcgcca atgtggtcga
    11521 catgttggca aaggctgtgc ctctttctct cctgatctgc cctaggcata tcggttgcgc
    11581 agtctgctaa tcgggggatg aaaccgatgt gagagtgtag ctgaattcga tatcaggcat
    11641 gatggtggat gtgaaggaga caactccgat acccttctta aaaaccctcg tctcgtgata
    11701 gaggccatcg ggcatctcag gatcaggagc agtatactcc accttgcagt agtccaacct
    11761 gtcctcggca tcgaccccgg tctcagtaat ctcagcgtcg acacggtttc cattcactga
    11821 ttcaaaagac cacttctgcc ccttttccag cggggctttg agcatcacaa ggccgtcgat
    11881 cctatgcgga aacacaacgc ctttgtggag tatgcgctcc acaacctcat cccctccgaa
    11941 aacatactga agttgaaagc cgaagtcctg ttctgactcg cctccggaca tgtcttccac
    12001 catgcctgat atctggtgta ccacgtcccc tttggagcgc acttccgtaa tgctatctaa
    12061 gatcattctg tgcccatatt cggcgaatcc tgagtatacc caggtgaagc ccgttttctc
    12121 cggaaaatag ctcctcagtt cgttgctgga agccttctgc cggaaacacc ctacaacgcc
    12181 caaaagcaac acgctagcca cagcgatggc tgagaaagcc cttttggtgt ccatccccaa
    12241 acccgccttt catccaatgg ataattgcct gagccaatcc ataactatgc tgccgcgaag
    12301 ccttcctctt attcattgtc cggcgcgacc ccttgatttt gtaatcccac tgcacgggtg
    12361 ctcttgcagt ccccactact caaccggatc caatggctct gataaggtgc cccacacgaa
    12421 tccatatgcc cttcgatctt gcccaatctc tgccgccaac ttagacacta caagcctgcc
    12481 agggccatgg tttgacaaca ggctcccgcg tcgataaact gacgaagtaa gccaataaag
    12541 cgggcatgag gtgataccga tcagagccta ctattctatt ggggaaatat cgaggctttt
    12601 cggcatgtcg atacaaaccc taaggcacta cgacagaatt gggctcctcc gcccagccta
    12661 tgtgaaccat gataacaatt acaggtacta ctcccatgaa caatttcttc agatcaagct
    12721 gataaagtac ctgaaaaccc tcggcttctc attggcccag atccgagaca tcgtcaacca
    12781 ggacctgtcg tccgaggtga taatcgaact cctccgtgag caagaagagg cggtcaataa
    12841 cagaatagac cagctcgtcg ccttgcggca agacatgcgc gccttcgaag aaaggctggc
    12901 cggcctcgca gaacggacct ttggagaggc tttcttagat agcaagcctg aacgaaaagt
    12961 catagcctac gattacatca acaaaccgaa tgcaaacttg gccgttggca tgaggaagat
    13021 tctcgcggat atcgagagag aacacagtgt actcaagtat gagccgggta tgctagtatc
    13081 atataccgaa ttcgcagcct caggaagagt agagttcaag aaaatcatag tcgagttaga
    13141 cgatgaactc gactatcctc accacaacat attgagactt cccgcaggtc cccacgccac
    13201 tatcatatat gagggcgcca cagaagaggg ccaaaaatac tacggcaaaa tagtagagtt
    13261 catatccgac catggcctaa gcccgaaggg cgatttcatt cacgctatag ctgtctccta
    13321 tgttaacagc aatggcacag gagtgtctct gcacgaggta tcggttctca cggaatgatc
    13381 ttctcccgtc agcttgactc tataccaaca atagactgta agctttcctg ttagggaggc
    13441 ctttcagtcg tgagcaaatt cttgcattat accgtcccat ccgtgatggg atgtttagta
    13501 acttcattgt atctcgtcgt agacggcgtc ttcattgcca ggggagttgg agaaacagca
    13561 gtagctgccg taacattagt gctgcctctc acaatggcct ttgtggcatt gagcatgatc
    13621 ttctctgtgg gcggaggcaa tctcgtatca acttcgagag gcgcaggcga ccaagcccgg
    13681 gcggtcaatg ttttcagaga gtcaatatac atgctgcttt cgataggaat tatcttcagc
    13741 atcctaggaa ccctgttcgc ggatgaaata gcgataatgc tgggtgcaac gggtgaactc
    13801 gttagcccgg ccgctgaata cacgaaatac tacatgcttt tcaccgtgcc cgtcctgctc
    13861 gccatcgcat tgagcacctt catacgacac gacggatctc caaagttgtc gatgcgttct
    13921 atgctgtacg gagcagtcac caacatcgtg ctcgactacg ttttcgtgtt ccccctccgg
    13981 atggggctgc gcggcgccgc aatagcttca gggctgggcc aaattgtcac cgtaatcacg
    14041 tgcatgaaac atttcatcga gcgacgcggg atcctgagaa taggcaaagc ctcattcgaa
    14101 tgggaagata tcaaagatat attcgtcctc gggttccctt cctttctcac tgaggtatct
    14161 tactccgtaa tgatgtacct gcacaatttg gcagtaatca gccaagtcgg cgagatagga
    14221 gtcaccgcct acggtatagt caactacatc aacaacatct cctacatggc cttgttcggc
    14281 atcggccagg ggattcaacc aatggtcagc tactactacg gacgaggcaa gttcgacaag
    14341 ggacgccagc agtatatata cgggataaaa gcgtctcttg tgatgtcagt cttgttcctg
    14401 gttatctgct tattcgtcgg aagaccactt ataatgatat tcgcctcgtc ggaagacgtg
    14461 atcgacctgg gttacacgat actgacatat ctgaacctgg cctacgttct gctgggcgtc
    14521 aatctgacta cgcagatgta tctgcaatcc atgcacatgc ccagatacgc caatatcatc
    14581 tgtgtgctaa gaggattcgt attcgtcaag attggccttg ccttactgcc gaaggtgttc
    14641 ggggatgcag gaatctgggg cacactattg tttgccgaag gaatgtccct aattacagcg
    14701 ctcgtcatag tcagcgccag cgccgaaccg gagcaatatc catctccgat agggaatagc
    14761 cagacagatc atgctagcag accctgaaaa ccggcgcgat tcgcctaaaa ccccgcacca
    14821 gcgccgaact ttgcaccacg tatgcccagt atggagtgat catggacgat actccttcaa
    14881 taccctcgcc atcctggcca tgtcttcctc cgaatatcgc tggtcgcaag ggagcgtcaa
    14941 gatgcggcgt gccaaccacg cactctccgg gtactcctcc tcagggacct ctggcggcaa
    15001 ttcccagtgg atatctgcat gtatcccgta atgttccaga tggcgccaga gcaggtcccg
    15061 atcttcaacc actacgggaa acccaagcgg gaccgcgtct tccgggagtt cagagatgag
    15121 tgcatgcacg cccccacatg ttgccagctc ctcaaggagt ctccgaaaat tcctgcgccg
    15181 aacccgcctg atctcttcga ttccgaccct tttcagtatt gacctggaaa caggcgacat
    15241 ggcggcgctc gcgctgaaac tgctatccag cgccgcctca gcctgagcaa acaggagcac
    15301 gtagtcccgg cgattcccga atccgtcagc cacgtaggcg ctcttcatca tcattgccaa
    15361 cgccctcgcc gagtgaaatc cgctcagctg cgcctcgttg ctacagaatg caggctcact
    15421 tccgtccagc cagacgcaga cggcaccgtc aacaaccgga aggctcttcc tgaggctcat
    15481 tatgcacaga ttcggctcaa aggtagtgtg cggccatggg cgttcacaca gggcgctgtg
    15541 ggtcgcatcg taaaccaccg caccgcccct tcgcctgatc tcagcgagag cggaaagcag
    15601 gtctactgag tcaggaaacc cgaagtaatt gagggccacc acgagtgtgc cttggatatc
    15661 gtcaaacgct ttttccatga gatcgtcggt atcgacagaa agatctgaga agacccgata
    15721 atgctcggtc tcaactccca ttctgtgaaa cgggctgagc atggagtagc aaaggtaaga
    15781 tggcaagagc gcccttcgca ccagacttcc gccttttttc aggttgagcc atgcattgag
    15841 gtaggcagtc cgccctgaag caagcatgct tacaggacgc cctttgctca gcccgtccag
    15901 tacgttttga tatgacggtt cgccattcgc acatcgaata tccgcaaaac agaaatctcc
    15961 gccgatctcc atcacgatgc tccctttcac cacaagcact gcatgttaag tataactccg
    16021 caccatccat cgcgcattat gaccttacta ttccacccca acaggtcgaa agccccttta
    16081 cagcactata tcggggcaat tgggttggaa cggagtcttt gcgacgaatg acaaaggctt
    16141 gcattcaaga tgagaatatg gatccctacg agtctaatcc gatgccgatt atgtgcattt
    16201 gagtcgtcgc caaactcgca ttgtctgatg cccggtccct agctgccggt attcgcgcac
    16261 aatcaaatct gctggcgccg cggtcatttg aatggtaaca ttgagaacag gtatagacat
    16321 gaaaatgcgg ggccgaaagg catcgacaac atccgccggc aagcgtcagt tgcatgcatt
    16381 catgcaccgc agagtgtctg cacgaatagc gcactcacca aatataggtg cgccgaggag
    16441 gaagctttga tgcagattgt aacagactac gccgccgaca tctcccccga acagcttgaa
    16501 ggtttggagg tacattacct tcctttgatg atcacggtag atggcaaaac gtaccgcagc
    16561 ggggttgaca tcactcctga ccaattctac gagttacagg caacggcagt ggagatacct
    16621 accacgtctc aaccttcgcc cggagaattc gccgatctct atcgcagcct tgcgcagaca
    16681 gatccggata ttctgtccat tcatatatcc tccgggctga gcggtacagt gaatgtcgca
    16741 agaatcgggg caaaaatggt gcctgaagcc gatgtggaag tatatgacac aaagactctt
    16801 tcagccgccc aagggtggca cgttgagatg gctgcaaagg ctgtgcgtgc cggatgggaa
    16861 aaatcccgga tagtggcgat gctcaagcag gtcactgaag cctcagagac cctcttcacc
    16921 ctctcaacat tgaagtatct aatccacggc ggacgaatca gccatctcaa aggactggta
    16981 gcatcgttgc tcaacataaa accaataatc ggcgtagaaa aggcaggcgg cacatacgtc
    17041 acacgtggac aggcacgcac gatgactagg gcaattgacg cgttagcaga tatcgtcagt
    17101 tcgatgtatc ctcctgagac gcccatgcgt gcgcagattc tccattcgca aaacccgaca
    17161 ggcgtatctc ggcttcagga caagctcgat aaggtcttcg tttgtaactg gctcccaaca
    17221 ggcccgatag ctccggtgct cggtgcgcat acaggcccgg gccttgtggg gatagccatt
    17281 gctcccgaag cactatttcc gcagatgcca taggaggcta tcaccgtctg cagcgaacaa
    17341 tcatgaccat gttaggagcg agcatatgaa actccgcgat cttgcgcttc gaattctcat
    17401 gatgatttcc ggcctgtcct tgataggctt ggggatagcg atgttcgtaa aagcgggcct
    17461 cggagcagat ccgataaccg ttctatgcca gggaatatcg atcacagcca acatcagttt
    17521 aggcagagcg ctacaactta acggactagc cctctttgtt gtattgctca tcatcggcag
    17581 agaatatata ggattgggca catttgcggg gtccttcgtc actggagcca tggctgatgt
    17641 gtttagcgcc gcaatgcgca gccctacagc cccttggcag tcgtggccca tactgatagc
    17701 atcgatagcg gttatcagct cgggagtggc aatctacctc tctgcagagc tgggagacgg
    17761 acctttggaa ggagtgatga tgcttatcag cgaccgcctc ggagcaccaa tcgcctgggt
    17821 caagatagca atggacattg ccgcaacagt tgcaggtatt gcgatggggg ccgtgcccgg
    17881 agtcggaacg atcgcttccg tcctcttaac cggccccatc atacagacaa tgcttcaggt
    17941 aatcaagaag tccatggctt aatggccaat tgagtcggag cgtgcctttt tccttcgact
    18001 gctaccacgc tacatctggc gcgtctatcg tcacaacagc cttcatgccc tcatacggaa
    18061 ctggtaactg aaccggactg tagaccactt cgactgcgat gtcatcgaca gtcataaggc
    18121 ccggacatgg gttcccatct gcagccctct gcctcgccaa aggcgagcat gcgcttggat
    18181 caacagcgaa atgatgacaa cattcctcag gaatcggctc aaacccgagt ctgcgcagaa
    18241 aacggggcca aagagtgctg cagttgccac cgtctgatat gggcagcaac tcgcactcag
    18301 atccaggccc cgccggcttg tgctcaacag gcactgaact tttcaaagct gctcggatga
    18361 cgccatcatc cccctgttcc atgggaaacg gttcaatggc caccaccccg gcgcgtgatc
    18421 gcgcatatct cagaaagccc tccaaagccg cggttccaat accacagttg cgataaggct
    18481 tttctacaaa aacgcattcc aagagaatga tatcgtgcct gtccgaatca gtgacatccc
    18541 ttcgtatctc aggaggctcc ggcacaagca agtcgcctgc cagggagact tccgggctga
    18601 actggctgaa catatcccgg taatcacatg gtttgcccac gatgatccag tcatcgatct
    18661 gaaaccatag gcctgctatt atccccacag tcgtttccga tccatcatcg ttcctgtcaa
    18721 caatgtgtcc gcatgtgcct ctcacgaacc tgccgatatc gccgttgggt gtaaaaattt
    18781 ggttgacgcc gattctaagt gcaatcatca cgacagcctc ctcccgtaga ttactgtgac
    18841 gagtaggcca ccgcatcata gcacacaggt cggcacaatc atgtcaccgg gctagtccga
    18901 cagctcacga ctttcttcaa acaaggacta ggcacgtggc gccaccgcca caaccagttt
    18961 ccagccgatt gttgcaacgt cgttcaacct agcttttcct ctcctatgtc cctcatggat
    19021 aaggctattc ggccccgctc tatatcgacc gacagcacgc gaaccttcac gatatcccct
    19081 atactcacca catccagcgg atgcctgacg tacctattgg aaagctcgga tatatgaacg
    19141 agcccatcct gctgtacacc tatatctacg aacgcgccga agtccaccac atttcgcaca
    19201 gtgccttcga gcatcatgcc cggacgcagg tcatccatgg tcagcacgtc cttgcggagt
    19261 attggctgtg gaaactcatc ccgcgggtcg cgaccaggcc gctgaagcgc ttcaatgata
    19321 tcttccagcg taggcaggcc aacatcgagc cgctcagccc aatcttgagc atccaaggcg
    19381 ctcagccttt gacaaagctt agtccgatcg cgttccgcaa cgtcgtccac ggcaaacccc
    19441 agttctgcca atagatgttc ggccacaggg taagattcgg ggtggatcgc ggtgttgtcc
    19501 aacggatacc tgccccccgg caccctcaag aagccggcgc actgctcgaa ggtcttgggg
    19561 cccaaccggg agactgaaag cagttcttct ctttcgccaa aaggcccttc ggactctcgt
    19621 tttgcaacaa tattggcggc tactgatggg ctaattcccg caacatacga cagcagagga
    19681 actgacgccg tattcaggtc gacacccacg cggtttacag cagactcaac cactccattg
    19741 agcgtagtgc tcagctcctt gccggaaacg tcgtgctgat actggccgac gccgatggat
    19801 cgcggctcta tcttcaccag ctcggccagg ggatcctgaa gccgacgcgc tatggagaca
    19861 gcgctgcgct ccgaaacatc cagatcaggg aattcttcgc gcgcaacctt ggacgcggag
    19921 tagactgatg ctcccgcctc atttacaacc acatacgaaa ttgaccgctc cgccttggga
    19981 atcaagccag ccacaaacgc ttccgcctcg cgggatgctg tcccattgcc aatggcaatc
    20041 aggtccaccc cgtgctcgtc aatgattttg agcaagactt gctcggcctc ctcgatcctt
    20101 ttctgaggag gtgtaggata gactacagtg gtctcaagca gcttacccgt tgcatctacc
    20161 accgctatct tgcaccctgt gcgatacgcg ggatcgaggc cgagcacgac cttgccacgg
    20221 atcggcggcg tcaggagtaa agccttcagg ttggctccga ataccctgat agcctgctgc
    20281 tctgcttctt ccgtcagcgc attacgcacg tcccgctcaa ctgcaggggc caacagacgg
    20341 tcgtaggcgt cttttgccac gagggccaat tcggcatctg ccggactcga cttcggccct
    20401 tctcgcatca acctccgctt caggtatgcc actataggct ctgcttcaag cgtgatacga
    20461 acgcgcaaga ccttctccct ctcccccctg tttatcgcca acactcgatg gggaggaatt
    20521 ctattcacag gctcgcagta atcgtaatac tgctcatagg tagtgcgcgc ctcagagtcg
    20581 gttgcttctg ttgatatttc gccatggcct gccgtccatt ggcgagccca tgcccgcact
    20641 tcaggatcat ctgccagaat ttcggctact atgtcgcgcg cacctgccaa agcctcttcc
    20701 gcgctggcaa cacccgcttc aagattgacg tattccgccg cccgctcgag aggatcacca
    20761 gtttcaggcc gcaagagcaa ccattctgcc aatggcccca aaccccgctc acgcgccatg
    20821 ctcgcccttg ttctgcgctt gggacggtat gggcggtata tatcatcaac acgttggagg
    20881 gtttcagccg tctccaacaa ctgccttatc tctggcgtca gcgccccctg ctcgtctatg
    20941 agcctcatca cctcgagctt gcgcgtctca agattccgca ggtaatcaag cctctcatct
    21001 atagagcgaa tggcttcctc atcgagtccc ccggtctgtt ctttcctgta gcgggctaag
    21061 aaggggatgg tgtttccctc atccaggaga cgtatgacgc tatccacctt ggatcttgca
    21121 attccaagct ccccgctgat cttggttgca atccccgccc acatctgcgc agcgatttct
    21181 gcctcattcg gagtcccttg tccttccatg tcctctgcgg tcgcacgtag tccatctttc
    21241 tcttgttctt cagttgccaa agactcgact tccatatctt aacttgcccc ctgtaatatc
    21301 caactcaatg cgtgcgatag tattccgcaa tcgccgattt aatcttagca tcctttttga
    21361 gtcagtgtcg aacggcgcgg ccatgcgcat acgcacaaca tggcgacatg gacctgtaac
    21421 ttgccatgac ataacatatc gtcacatgcg ggacaaagaa cggtaaaggc ctgccagaat
    21481 gccttcttgg caggcccttg ccccatgtac tacgactcaa tctgcataca taactaacgc
    21541 taatcggata acagtcagat tcctaacagc tctcttgcga tagaagcgat atccgttatc
    21601 gagctcacgt tttctatgtc cacactatcg gcgttccgac ctactacaat caaagggaca
    21661 tcaacgtcag tatcgagggt agtcccgtgg cttcccagtg tgtaatccat gaataacaat
    21721 gcctctataa acatgcagcc acggtcagtc actgcgatca gcggaggggt gcgaggatgc
    21781 tctatgtgga gttctgcgcg tctagccacg tcttcaacct ctatcacttc agccacgccc
    21841 tcagtctgtc tcagcgcatc cgcgatcttc cccctgttat cttcggccca aacgtatagg
    21901 actccgccgt caagagttat tggcacatct tcatcggtga tatcgccgac gatgtccctt
    21961 gccgcgatgc cggtgtcgac cctagtcatg ccgtggtctg ctgtgatgat caaactccac
    22021 cgtcccggac gtgtcgcttc cagtgttgca gccaattcct caagaccttc gctgatagag
    22081 cagaggcact cagctgtttc ggcagcgggc aaaggcccga acctgtgtcc acacttgtca
    22141 acgctcccaa cgtttatgcc gacaaataca ggcatgtcat tctgctctag ataggcctcg
    22201 accgtcctca aaacccaccg atctagttgg atatgccatc cggcgaaatc ctcatactgg
    22261 gtggactcat cgggcggagg acccacagtt tcgatgaccc aatccggtac cgactcgggc
    22321 cagattgcga agtcagtagc ctccgagagc atcttgcaca agttagcttt tgcggagatc
    22381 attgcggtac ccaaccctgc agagcgcgcc acatggaaca gcgtcggctc ttccaaaacc
    22441 gcaggatcct tgaaccgcaa tagctcccca tcatcgtcta gatactcctt gcctgttacc
    22501 ccatttctct ccgggtatgt tcccgtgaaa attgccgcat gcccggcggc tgtcgaagac
    22561 gggaatactg cctcaatcga cttatactgg catctctcgt cagcacacag ttgcctgagg
    22621 tacgcggttt caggcaagca gcccagtgac aagttagggc acccatctat cacgcagact
    22681 accaccgtca tctgctcctg ctgctctgac ccggcggcgc tggttgccag cgccgcctgc
    22741 ggccaggcta tgattatggc caatagcagc agtaacagga acctgcaatg ctgcttgcgc
    22801 aatgtcattt cgtcctcact tcaatggtcg tgacaaacca gttccagctc tccttcggat
    22861 actgcttgtc aaggcctggg ttgtcatcat acgggaaaac aagcttgacg gggccaccaa
    22921 gccctgatcc gattgctttt ccgccatctt tcgtggctac catgatctcg tacttcgcaa
    22981 cgtccgcagc gcttataatg acctctttgc cgtcctttgc gattaccacg acctcactta
    23041 cgttcttcgg cgctcccacg tagcccaata tcttggcgat attcacgcca gcgtatgttt
    23101 ttttgccaag ccagggatct ttgacctgat actcagtgag tcctagtccc tcaagcatct
    23161 ccaagtcaaa ctcgcatgcc cctttagcat tggtattggc tatagggcct ttcacggtca
    23221 ggaccacctt gccgctgggt ttcgcgggac tcgcgaagca catcgaaccc aagattacga
    23281 acgtcaagca aaagacaaca gctagtctgt atttgcgcat ttcgtctctc cctttcgata
    23341 tggttttggc gcaacgccat ttcagttgat cgcaagattc cttgacccaa gttcccacgg
    23401 ctatccttgc cccttacgca cctccccgac aacatgtatg gtagacccct gcctgatcag
    23461 catcgttcct cccactacca acaagagagc tgcaatgtag aatggcgtgt tttccgatgt
    23521 ggaatagaaa aagccaccca ggtagggtcc tacaatcgac gccccccaag ttgccaaatt
    23581 gaagagggca aaagcctttc cgtctgcgcc atcgccgagg acctccccca cccctgcaaa
    23641 ggcaacgcca tggccagcca tgaatccgcc cagaaggaag aggcatatga ccacagcaac
    23701 ctggcctgaa atggcgatga aacacagcat cgagatgaca aagacaatca cattggctcc
    23761 taccgctaca agagtccccc gattgtctgc cattcgccct accacgacag ttatcattgt
    23821 ttgcccaaga gacattatcg aaccaagcag actgacaatg cttatgtttg tgtggcgcac
    23881 gtcgctgaga tactgcggaa ttatctgctg caccatgtaa aaagagaaag cacacagaga
    23941 gtagcagaat acgaacttca tgaacgatcg gtcggccagc agacttgcat atgtagaaac
    24001 gcttgcagtg ctctcacttt cgggttctgg tggcaggaac gctatcacgc aacatgctgc
    24061 tgcaaagaac gcgaacgaaa gaaggaacag attctcgatg ggccaaatgc tagacagata
    24121 agcggataga atcgggctcg ctatcattcc ccctgaaaat cctgcgctta tcagcccaaa
    24181 cgttcggccc gactcgcctt cgggagaaca agccccagca tatgcgttga gtgcggggta
    24241 gcccaacatc gaccctgagt aaagagcaat gccaagagca gcaagtctcc agtctttcgc
    24301 gaaatagtac aatattacag atggcacgcc cataatccac gacaaaagga tcactctttt
    24361 tcggtcatac ttgtcggcca gcattccccc tggtatgctc gacgccgcca taaccgccat
    24421 ggatattgag tagacgaatc ccacttcctg tggggtgcat ccaatgttgc ggagatacac
    24481 aggaaacatg tatgaatgaa gaccatatcc gaatccccaa agcaacatgc ctgcagctat
    24541 taattgtatg cccttcggcg tacgactcgc ttgcgactcc accaacgtta cctccgatca
    24601 atgctgcgca ggccaggctg gttctgcact attccagaac tcctgcaatg cactttccgc
    24661 cctacaaaac gccgcgtaac cacggtcata tccatcaccg cgcacgccct ccggggtgaa
    24721 tacagcgtcc gtgagaacta caacctgaac agcctctttg atgctgctga atacatttgc
    24781 agcagttcct gccagaatcg ctgcgcccag aagtgtgcct tccttcacct tcatgcttgc
    24841 caccggcaga ttgagcacat cagccttcat ctgcaaccaa cctcggtgcc ttgcgccgtt
    24901 ccctacagcc ctaacatcca ccgctttcat cgaatcctgc cacgcctcca tgctctggcg
    24961 catggcatag gcacagcctt ccaaaacagc acggatcatg tggcaagaat catgctccgg
    25021 tcgcagtcca agaaaagcgc ctgtcgccct tggaaccgca acggggctgc gtttgccgac
    25081 gaaatatggc agaaacacca agccagacgc tccagattct gagcaatcga gcgcttcagc
    25141 gaccgtgtcc ctccagtcga gcttgcttga gtacacttct gggtagagaa cagacatggc
    25201 ccaggcaagg catccaccac tgtaggatat tggggaaact atcaatcgct ctccgggaat
    25261 gacatgtttt acagtctgta ggctgcatcc atcaggagcc actgcagaaa cgcatgttga
    25321 cgtgccggtt gagtcgcata ttagcccggg actagtcacc cctgcgccca gagcctcgac
    25381 gaacgcgtcg atgcttcccg cagcaaccgg tattcctgag ggaagccccg tctcgtctgc
    25441 agcctcgcgg gttacctccc caatggcctc ccacggatcc accaccctcg gaaacagcct
    25501 ggctccgggg gcagatgagg taatatcacc tgtatcgtct gagtgccacc tcatacacga
    25561 cgccgcagaa gaatcgatac acgtctcgcc tgtaagcttt aaccccacgt a
//
LOCUS       DAOSYL010000032        25344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1605_length_25344_cov_8.030567, whole genome shotgun sequence.
ACCESSION   DAOSYL010000032 DAOSYL010000000
VERSION     DAOSYL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 25344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25344
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1605_length_25344_cov_8.030567"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1586
                     /locus_tag="PLU15_06680"
     CDS             213..1586
                     /locus_tag="PLU15_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO30644.1"
                     /translation="MQNLIKDALKSHRADYVEIRIEDHERTKISLRGKNVDELATSRS
                     VGGCVRALVSGGWGFVSFNDIANLKDHVALAVSQAKLVSRVCGDNLLAPVEPITDTVV
                     PALVVDPRSISLASKRALLEEYNDIMLSYSPKIQTTAVRYRDTYSKKYFANSECTYIE
                     QEHCDVGASFTAIAREGDNVQQMLVSAGGIDGYQCIKGHHDEVRECARRAVENLSAAP
                     VDAGQYTVVLDPGLAGVFVHEAFGHLSESDFIYENSMLREIMVLGKQFGSPILNIADG
                     AAVPGHRGSYKYDDEGVPSSVTPLIRDGVLVGRLHSRGTAAKMSERPTGNARAINYRF
                     QPIVRMTNTYIENGETSFDEMISDIDKGIYAIDAYGGETTMEMFTFSAMEAFMIRKGK
                     VAERVRNVVLTGNVFETLMHIDAIGNDRKMNDGGVGGCGKGQQFPLPVSDGSPHIRIK
                     NVVVGGN"
     gene            1589..>2220
                     /locus_tag="PLU15_06685"
     CDS             1589..>2220
                     /locus_tag="PLU15_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOO30645.1"
                     /translation="MKDLLDVAIKNADAAELYLVNSETIPVVFEMSKLKQAETKRTTG
                     VALRIIRSGKIGFSSSTDLSKAAADNLVNMATESADMGETARFDFPGPITDGEVMPAP
                     LIYDDRIADIDADELIRMGQEIVDIITEEDPSIKCGVQLEREVQEVTITNSNGGGGRY
                     RKTTLTAMADALLVEGTDIFLIYSMAGSCCSNFDSRSIAQDVLERVRLGRT"
     assembly_gap    2221..2320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2321..2975
                     /locus_tag="PLU15_06690"
     CDS             <2321..2975
                     /locus_tag="PLU15_06690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOO30646.1"
                     /translation="MPLFIALQSALNGKTVLEGSSPLADKVGRQVFDPRVFVYDDATV
                     DCALGSCPMDAEGVKSRKTPLIEAGVVKGHYYDLQTAGKAGTSSTGNGVRQAHAGDYF
                     EGQPTPGTSNPLITPGTESLEDMIASMDEGIIVDQVMGAGQGNVLTGDFSVNVHLGFK
                     VEHGKIVGRVKDTMVAGNTIKALNDIVAISDRAEWAYGQYSAPAFLFRSLGIAAKGS"
     gene            complement(3269..3946)
                     /locus_tag="PLU15_06695"
     CDS             complement(3269..3946)
                     /locus_tag="PLU15_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOO30647.1"
                     /translation="MPIRFILFDLDETLYPASNGLMNIVRDRIIEYIMLRLGVSRREA
                     EEYRLRYFMEYGATLGGLYHEHGVDVRDYMEFVHDVPHSDHIDPNPELDAMLSRLSPA
                     KVIFTNADRSHAQRVLSALRVTPGHFNCIFDYEAAGCCPKPELEAYRRILDYLDAEGA
                     ECILVEDNLRNLAPAKRVFGIHTVLVDSDGTRRIRGGPEYAADWVIRDILELEGLLEQ
                     VYGRAGR"
     gene            complement(4225..4869)
                     /gene="sigH"
                     /locus_tag="PLU15_06700"
     CDS             complement(4225..4869)
                     /gene="sigH"
                     /locus_tag="PLU15_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOO30648.1"
                     /translation="MSVNLQYEIYLHYDELADEEIVELAKQGDSDAEEYLIHKYKNFV
                     RAKARSYFLIGADREDIIQEGMIGLYKAIRDFKPDKLASFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYVSLNKPIYDEESDRTLLDVLSGNKITDPEELVISREEFNDIEDKMD
                     EFLSDLERDVLNAYLDGKSYQEIAIELNRHVKSVDNALQRVKRKLERYIKKRDG"
     gene            complement(5240..5998)
                     /gene="rlmB"
                     /locus_tag="PLU15_06705"
     CDS             complement(5240..5998)
                     /gene="rlmB"
                     /locus_tag="PLU15_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOO30649.1"
                     /translation="MDDEFIIWGRQPVMEALESDREFNKIFVARGGHDKRIVAAARER
                     RIPVVEVDKAALDRMTASETGVPVVHQGVVAQLSPILYAELDDLAAAAQARREDLFVA
                     VLDGITDPQNLGSIIRTANAVGAHGVVIPARRSSQVSPVVAKASAGAVAYTPVARVGN
                     LSQAIDRLKKMGVWVVGTAADGDRRFFEADLTGPIAVVVGSEGKGMSRLVEESVDFTV
                     SIPMAGEVSSLNAGVAWAVIAFEVARQRLGVNRG"
     gene            complement(5991..6389)
                     /locus_tag="PLU15_06710"
     CDS             complement(5991..6389)
                     /locus_tag="PLU15_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOO30650.1"
                     /translation="MSLLNLAYVGDAVWELFVRSQLAAEGGACIRVNALHRSAEEMVN
                     AEAQSQIVHKLVDFLSPEERDVVRRGRNVKPGHVFRGPDPIDYRYATGFEALLGYMYL
                     AGKDERLREIMEEAMRLAQQKDGVEEPANG"
     gene            complement(6406..7938)
                     /gene="cysS"
                     /locus_tag="PLU15_06715"
     CDS             complement(6406..7938)
                     /gene="cysS"
                     /locus_tag="PLU15_06715"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOO30651.1"
                     /translation="MAIRIYNTLTRRKEEFVPVVPGKVGIYSCGPTVYDYFHIGNARA
                     FVVPDVIRRYLRYRGYNVTLVQNITDIEDKIIKRAAERGVAPQDIVDEFTTAYIEDRE
                     ALGVQAPDIQPRATEHVGDMIDMIKILVEKGLAYVGDGDVYYDVSRFEPYGKLSNQNI
                     DDLVAGARVDVGEAKQDPLDFALWKAAKPGEPSWDSPWGPGRPGWHIECSAMSTKYLG
                     KTFDIHTGGVDLVFPHHENEIAQSHGANGVAPVRYWVHNGYINVDGQKMAKSLGNFKT
                     VRDILQQYPGKVVRYFLISNHYRKPINFSDDELRMCERALSRLEDAIVNAAFTASIDL
                     DNREEMVRLAAAKSGKAWADDSAEARLDGVVKEVRRRFEESMDDDFNTAGAIGSLHDL
                     ATALNTYVNREMPSPPNEEARVAISGAVLALMDLGDVLGIIDPSSMKPAAGNAKEDEG
                     ELANAEKIVEGLVELLLDVRARAREAKEYGVSDMIRDRLAEMDIIVEDTRDGARWKFA
                     SK"
     gene            complement(7991..9280)
                     /gene="ispD"
                     /locus_tag="PLU15_06720"
     CDS             complement(7991..9280)
                     /gene="ispD"
                     /locus_tag="PLU15_06720"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOO30652.1"
                     /translation="MGARLRVTAIVPAAGSGTRMGLAVPKQYLQLRGIPIVAHTVSRL
                     ARSGAVDEIIVSVPPGDEEVFERDIVERYSLSSLCRLKVVAGGSDRQQSVSLALRAME
                     QDLASSATGTEGRGAGVEEAGVDAETCHIVLVHDGVRPFVSKAQIVAVLEAAAVHGAA
                     TCAVAPKDTIKLVGDNGLVAMTPDRSGLCLVQTPQAFHLSILACAHRRAEEEGYVGTD
                     DCSLVERLGLPVALTPGSYDNIKITTPEDLALAEVLLAREDSDCRGESVVSVVRVGQG
                     YDVHRLVHGRDLILGGVQVPYEKGLDGHSDADVLTHAICDAILGAASLGDIGMHFPDT
                     DQEYAGICSVALLERVVALAADAGYRVLGVDATIIAQRPKIAPFVPQMRESLAYAMNV
                     PVEYVNIKGKTTEGLGYIGSGEGIAALAVASLSPASS"
     gene            complement(9332..10450)
                     /locus_tag="PLU15_06725"
     CDS             complement(9332..10450)
                     /locus_tag="PLU15_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOO30653.1"
                     /translation="MSVRNKMTRVLMLGAGVIFGYLIYRAMPQTGAALSVEGGELRVM
                     VGAMTGGGLVGFAVAPVISRFGLWFGRYVDARIRKVSTHGVISGAVGMIVGLIIANLL
                     AIPIASIPEFGSYIGIALNVFFGVSGLIVGYHKREEIIPMLAPLPRSGENVHVSAPAR
                     DRGIARAYVGKATPKILDTSVIIDGRIADIADSGFIEGTLIVPVFVLEELQHIADSSD
                     VLKRNRGRRGLDILNRMQKGQNVTVQIVDETFDDPEVDSKLIRLGQAMDATILTNDFN
                     LNKVAELHGVEVLNINELANALKPVVLPGEEMTVHVIKDGKESGQGVAYLDDGTMVVV
                     DGGRRYIGDYVDVLVTSVLQTSAGRMIFAKMKSAERVS"
     gene            complement(10537..11013)
                     /locus_tag="PLU15_06730"
     CDS             complement(10537..11013)
                     /locus_tag="PLU15_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOO30654.1"
                     /translation="MYSVGDKVVYPMHGAGVIEAIEEKEVLGEKDYYYIMRLQMGDMK
                     VMVPRGSADEVGLRPVIDCDEVPKVYEVLKAEATPMSQNWNRRYRANMEKMKTGDIFI
                     VAEVVRNLGIRDRDKGLSTGERRMLDNAKQILASELAMVEKRPLEEVANVIDEILS"
     gene            11260..11655
                     /locus_tag="PLU15_06735"
     CDS             11260..11655
                     /locus_tag="PLU15_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOO30655.1"
                     /translation="MADLSCGEFQETVSEYLVRHRSVLDVMSKLQEAGARVNRAVAKS
                     VTSCGCLEVSAQKQKFPENATLADLKRMINSHISGELCPECREALETELGQMLFYIAA
                     LCAITGLDLDEIMMKEHDRLRMLGLFNLT"
     gene            complement(11713..12771)
                     /gene="disA"
                     /locus_tag="PLU15_06740"
     CDS             complement(11713..12771)
                     /gene="disA"
                     /locus_tag="PLU15_06740"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HOO30656.1"
                     /translation="MSERMRKIFQMVAPGTALYDGLENILRARTGGLIVVGDTPEMMK
                     LVNGGFRLDAEFTPAAVYELAKMDGAIILSEDAERIMYANATLTPDPDIPSRETGLRH
                     STAERVSKMTGQLVIAISQRRSVITVYYGHTRYVMRDIGVVLAKANQALQTMANYRDS
                     LVKALTNLTALEFIEEVTLVDVARVVQRYELMRRVGEEVSGYIAELGTEGRLVKIQAD
                     EVMSGMAEEVLFVAMDYMPDGGQVEAEKLMNEIAGWAVDEVLDLATLSRALTQNPSAT
                     PESSMSPRGYRALSKISRLPLSVIGNVVEGFGSLKAIRNATVEELDEIEGIGEVRARA
                     VREGLARLRDQVVRDLVI"
     gene            complement(12771..14180)
                     /gene="radA"
                     /locus_tag="PLU15_06745"
     CDS             complement(12771..14180)
                     /gene="radA"
                     /locus_tag="PLU15_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOO30657.1"
                     /translation="MKGAKVAFVCQECGYESARWMGKCPACDSWNSFVEERRPDVRVS
                     RPISSVGAGWGGSGAVAAPVPIGQISASGEKRLSTGIGEFDRVLGGGVVAGSVVLIGG
                     DPGIGKSTILLQAAQSIAASKGVVLYVTGEESVEQVGLRAARLGAGSDSLLVLAETDV
                     RAVVEAMVTIDPVLVVIDSIQTMEDVELGSSPGSIGQVRECTNRLMRAAKERGVPVFL
                     VGHVTKSGAIAGPKALEHVVDTVLYFEGEGNHAHRIIRAAKNRYGSTNEVGIFVMTDR
                     GLAEVENPSEALLSERPGGAAGSVVVASVEGTRPLLVEVQALVARSVTGTPRRTTTGV
                     DYNRTAIIIAVLDRRAGFAIGTEDIYVNVAGGVTLTEPAADLGIAAAIVSSFRNIPAA
                     PKTAVFGEVGLAGELRGVSQADMRVNEAKRLGFKRCVLPSHNVKSMTRRQLAGSGVEI
                     VGASTLREALEAVFAVVDG"
     gene            complement(14220..16685)
                     /locus_tag="PLU15_06750"
     CDS             complement(14220..16685)
                     /locus_tag="PLU15_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOO30658.1"
                     /translation="MFGSFTERAQRVLYLSQEEARKLGHSFVGTEHILLALTCESQGV
                     AGRALHNMGVEPEAVRDEVESIIGRGEPGQAEMVGLSPRAKRVLELSIDEARLMGHGY
                     VGTEHLLLGLLREGEGVAAQVLENLGVDLDRARAEILDLLGGGVSGSSQRQEAQGQAG
                     AKLQRRKGTPTLEQYGRDLTAMANEGKLDPVIGRETEISRVIQVLSRRTKNNPVLIGD
                     PGVGKTAIVEGLALKLNRGDVPELLKEKRVIQLDMGSLVAGTKYRGEFEERLKKVIDE
                     IIASGDVILFIDELHTVVGAGAAEGAIDAANILKPALARGEMQCVGATTIDEYRKYIE
                     KDAALERRFQPIQVGEPSVEETVEILDGLRDRYEAHHRVKITDGALRAAAVLSDRYIT
                     DRFLPDKAIDLVDEAGSMVRLAAHVAPPDIKRLEDDLERVRTEKEAAIQNEEFEKAAE
                     LRDHEQSIRDTIEEQRKQWQNQRYLEGAVVTENQIAEIVASWTGIPVMQLEESEAERL
                     LKLEDILHKRVISQDEAVTAVARAVRRAHAGLKDPRRPVGSFLFLGPTGVGKTELARA
                     LAEALFGDEDAMIRLDMSEYMERHTVSRLIGAPPGYVGYEEAGQLTEKVRRRPYSVVL
                     FDEIEKAHPEVFNILLQVLEDGRLTDGQGRTVDFRNTVLIMTSNVGATQIHRDTVLGF
                     RTEEDEKNSYERMRDKVLSELKRTFRPEFLNRVDEVIVFHALGRKDMEEIVDLMLGDL
                     KAHLEPRGIHLTFTDGAKSLLVEEGYNPDYGARPLRRVIQRMVEDRLSDEILEGNIRD
                     GQSVEVNEAEGKLVFKALELQRE"
     gene            complement(16686..17753)
                     /locus_tag="PLU15_06755"
     CDS             complement(16686..17753)
                     /locus_tag="PLU15_06755"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOO30659.1"
                     /translation="MAAKRPYEKNRAVWMAGDGPDADIVVSSRVRLARNLRGVPFPQQ
                     ANDEQMKSVLEQVMGAVRATPSIGPTEILALQELLPIERQLAVEEHLTSPQHIQDPKN
                     KAIVLNRDKSVSIMVNEEDHMRIQTILPGFQLEEALRLASVTDDAVEETIDYAFDENL
                     GYLCACPTNVGTGLRASVMVHLPALALVGMVGQVLGAVSKVGVAVRGAYGEGTEAIGN
                     MFQISNQVTMGRAEEEIVAHLKAVCGQLADSERNARKAIMSDARNQLEDRVWRSYGLL
                     TNARIISSDEALKLISDVKLGADLGIIPDLPQSCFTVLSVDIMPAHVQDFVGKELTAW
                     ERDMCRATLIRERLGMNKEEH"
     gene            complement(17731..18327)
                     /locus_tag="PLU15_06760"
     CDS             complement(17731..18327)
                     /locus_tag="PLU15_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOO30660.1"
                     /translation="MICQVCREKPATVHVTKVVNNVRTELHLCQQCARERGELHVLST
                     PSFAISNLLAGLLQGDLGHEVHVGIPDGAGRKCRTCGRDYSEFVNSGFLGCADCYSEF
                     APLLNPLLGRIHGHVRHTGKAPRRAGKAVRFRREIDDLRRRLQQYVEAEEYEKAAIVR
                     DEIRRLEKAAAEVEIDGTGGDAEAKEKGGSGDGGEETV"
     gene            complement(18345..18818)
                     /locus_tag="PLU15_06765"
     CDS             complement(18345..18818)
                     /locus_tag="PLU15_06765"
                     /inference="COORDINATES: protein motif:HMM:NF017648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOO30661.1"
                     /translation="MGSLSDYIESYLKLLLDTGPHGFVEVQRRELAERFRCAPSQINY
                     VLSTRFTVERGYVVETRRGGGGYIRIFKLDDEPETSGIQMVYESVGDAIDRKAAEDLI
                     ARLVDMGALDCKDAACVRAAVFDQESDEDPELEGIIRARVLRAVLMLMLTRPPME"
     gene            complement(18993..20252)
                     /locus_tag="PLU15_06770"
     CDS             complement(18993..20252)
                     /locus_tag="PLU15_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO30662.1"
                     /translation="MLSTAVSDHQGRVFRNDNLHAAARTGNTLVELLESDVIPMPEGG
                     SLMRLPSRRPVGIDATSGQFVSAGLEEAVAAVLPQGYTRTFLPAYIGLEGAPQLPLFG
                     YSAVAFAGDQLMVAAIRTDERDTWDPIHYNLPELAEDIERRLRQFPENRILAQLARCA
                     TEYQCFTAQNVFYGRWEGGIPVSPTCNANCLGCISLQASECCPSPQQRIDFVPTVSEI
                     VEVALPHLEKAVGGIISFGQGCEGEPTRQWPTIVEACRELRQRTDAGTINVNTNAGDV
                     HAIEAICDAGVDSLRVSLASARPDYYGRYHRPRGWCLADVESSIIAARSRSLFVSLNY
                     LVFPGFFDQEPEVDALCDLIERCDIQMLQLRNLNIDPDIYADVMLTENVDSDSFYPAG
                     VDEAIAHIRDCFPSLAIGNFTMPIQRK"
     gene            complement(20282..21424)
                     /locus_tag="PLU15_06775"
     CDS             complement(20282..21424)
                     /locus_tag="PLU15_06775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO30663.1"
                     /translation="MPKQGLAAFMCVLVISFCVPTFSCGAMAAGSDEVSFSTDVMRRS
                     GALELVKAIFSRSWTILLAALGAKSDVVADWESGLYPRKPRLDPGANIISTLKTESEL
                     IGPSVAATNALDLDAGKQGSSSSFARAQASIVTHRVEQGESLWTIANSYGVSIETISW
                     ANSLANPNKLKVGQVLTFPSVTGVIHEVSRGDTVWTIAKRYGVSRDKIVEANAIADPD
                     ALKVKQSLVIPGGKPPVTSSRIVVASRGSSDGRISGAASSGGSLSWPVSGRITSRFGP
                     RWGRMHNGVDIAVPTGTSVSAAAAGCVIFAGWRGGYGRLVILDHGNGLQTYYGHNSAI
                     TVSRGEQVAAGERIALSGSSGISTGPHVHFEVRAGGKPQDPMGYLP"
     gene            complement(21654..22937)
                     /gene="rho"
                     /locus_tag="PLU15_06780"
     CDS             complement(21654..22937)
                     /gene="rho"
                     /locus_tag="PLU15_06780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOO30664.1"
                     /translation="MNIAELEKMTVPALHEIAKQLGIPGHSQLRKQELTFEITKAAIQ
                     KEGALWADGILEVLPEGFGFLRVGKMTPTSDDIYISPSQIRRFGLRKGDMVSGQVRPP
                     KDGEKYFALLRVEAVNGLDPELAARRSHFEDLIPVYPDERLKLEHDPKDVTTRLIDLM
                     APIGKGQRGLIVSAPKAGKTTILKKIANALTYNHPELTLLVLLIDERPEEVTDMERSV
                     DGMVVASTFDQPSSNHVRVADMVLERAKRLTEHGKDVLVLLDSITRLARAYNLEEPPS
                     GRTLSGGVDPAALHHPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKGTG
                     NMELHLDRKLAEKRIFPAIDINKSGTRKEELLMDAEELETMWLLRKAMATTGSTEMTQ
                     LLIDRIQRTKSNEELINIIRKSQFAENVRNWHNSM"
     gene            complement(23219..24832)
                     /locus_tag="PLU15_06785"
     CDS             complement(23219..24832)
                     /locus_tag="PLU15_06785"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOO30665.1"
                     /translation="MAKYVFVTGGVVSGLGKGITAASLGRLLKARGVEVAALKFDPYI
                     NVDPGTMSPYQHGEVFVTEDGAESDLDLGHYERFMDINVTRDNNVTTGSIYSAVLAKE
                     RRGDYLGGTVQVIPHITNEIKERVTRLGRDGAEVVIVEIGGTVGDIESLPFLEAARQL
                     RTEAGRENVMYIHVTLVPYIACAGEFKTKPTQHSVKELRSIGIQPDVIVARSDRILPP
                     EIKNKIALFCDVEPEAVIFNPDADSIYDVPLILEQEGLDRIVIRRLELPCSENRADMC
                     SWREFVKKSKNLSDSVSIALVGKYVSLPDAYLSVVEALRHAGIDCGVQVDLNWVDSES
                     ISSELVAEEALRGADGIIAPGGFGPRGVGGKILAAGYARKHGVPFFGICMGMQCAVIE
                     FARNVCGLSGANSTEFDPGTPHPVARLMADQREIEHIGGTMRLGVYPCVLDPASKLAK
                     AYGENVVEERHRQRYELNWEYAQVLSEYGMRISGTSPEGDMIEAFELMDHPWFVGTQY
                     HPEFKSRPGKPHPLFREFIVKSMEYSKSR"
BASE COUNT         5803 a         7676 c         5947 g         5818 t
ORIGIN      
        1 cgcaaaaact tattgggagt cacttagcca catctgttcg attgccaacc gcaagttcgg
       61 acagggcata tgggtcgcca cactactgaa ggataatcaa gctcaatgtt taagttctgc
      121 acatgctccc aaagcaaagc gcgacattcc catccaaaca tatctcccgc aaaccaggca
      181 aaaagctcag cccaaaaagg aggcacaaga tcttgcagaa cctgatcaag gatgcgttaa
      241 aatcgcatcg agcggactat gtggaaatta gaatcgaaga ccatgaacgg accaagatat
      301 cgcttcgtgg caagaacgta gatgaattgg ccacgtcccg ctcagtagga ggctgcgttc
      361 gcgccttggt atccggcggt tggggattcg tctccttcaa cgacatcgcc aacctcaaag
      421 accacgtggc gctggcagta agtcaagcaa aactggtatc gcgcgtgtgc ggcgacaatc
      481 tccttgcccc agtagaaccc ataaccgaca cggtagtgcc ggcattagtc gttgacccca
      541 gatcaatcag ccttgcaagc aagcgagcac tgcttgagga atacaacgac attatgttga
      601 gctacagtcc caagattcaa acaactgcag tcagatacag agacacttac tcaaagaagt
      661 acttcgccaa ctccgagtgt acatacatcg agcaagagca ttgcgacgtc ggagccagct
      721 tcaccgccat cgcacgggag ggcgacaatg tgcaacagat gctggtctcc gccggaggaa
      781 tcgacggtta ccaatgtatt aaagggcacc acgacgaagt cagagagtgc gccagacgtg
      841 cagttgaaaa cctcagcgca gcacctgtgg atgcgggcca gtatactgtg gtactcgacc
      901 ccggcctagc aggcgttttc gtgcacgagg ccttcggaca tctaagcgaa tctgacttca
      961 tctatgagaa tagtatgctc cgcgaaatca tggttctggg gaagcagttt ggctccccga
     1021 tcctcaacat tgcagacggc gccgccgttc ccggccatag aggatcatac aaatacgacg
     1081 acgagggcgt tccttcctcg gttactcccc tcataagaga cggagtgctc gtcggcagac
     1141 tgcattcccg gggaacggcc gcgaagatga gcgaacgccc cactggaaac gctcgcgcca
     1201 taaactacag attccaacct atagtgcgga tgaccaacac ttacatcgaa aacggagaga
     1261 caagcttcga cgaaatgatc tccgatatcg acaaggggat ctacgccatc gatgcctatg
     1321 gcggagagac aactatggag atgtttacat tcagcgcaat ggaagccttc atgatccgca
     1381 aagggaaagt ggcggaaaga gtccgcaacg tggttctcac aggcaatgtc tttgaaacat
     1441 tgatgcacat agacgccatc ggcaacgacc gcaagatgaa tgatggcggt gtcggagggt
     1501 gcggcaaggg ccaacaattc ccattgcctg tctcagatgg cagcccgcat atacggatca
     1561 aaaatgtcgt tgtgggagga aattagccat gaaagacttg ctcgatgtgg cgatcaagaa
     1621 tgcagacgca gctgagctct accttgtcaa tagtgagacc atccccgtgg tcttcgagat
     1681 gagcaagctc aagcaggccg aaacgaagag gactactgga gtggctctgc ggatcatccg
     1741 ttctggcaag ataggcttct cctcctcaac agaccttagc aaagccgctg ccgacaatct
     1801 cgtcaacatg gcaacggagt ccgcagatat gggtgaaaca gcaaggttcg actttccagg
     1861 cccgatcacc gacggagaag taatgcctgc gccactcatt tacgatgaca gaatcgcaga
     1921 catcgacgcc gatgaactca tccgtatggg ccaagaaatc gtcgatatca tcacagaaga
     1981 agacccctcc atcaagtgcg gtgttcagct tgaaagagag gttcaagaag tcacgatcac
     2041 caactcgaac ggaggaggag ggcgctaccg caagactact ctgactgcca tggccgatgc
     2101 cctgttggta gaaggaaccg acatcttctt gatatactcc atggccggtt catgttgcag
     2161 caacttcgac tcccgctcca tagctcagga cgtgctagag cgcgtgaggt tggggcgcac
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catgccgctt ttcatcgcct
     2341 tgcagtcagc ccttaacgga aagacggttc ttgaaggttc atcaccgttg gcagacaaag
     2401 taggccgcca ggtgttcgac ccaagggttt ttgtatatga tgacgcaact gtcgactgcg
     2461 ccctcggaag ctgcccaatg gatgccgagg gcgtaaagtc gcgcaaaaca cccctgatcg
     2521 aggcaggtgt cgtcaaaggc cattactacg atcttcaaac cgcgggcaag gcaggcactt
     2581 ctagcacggg aaatggcgtc agacaagccc acgccggcga ctacttcgag gggcagccga
     2641 ctccaggcac gagcaatccg ctgatcaccc cgggcaccga gagccttgaa gacatgatcg
     2701 cgtcgatgga tgaaggaatc atagtcgacc aggtaatggg ggccggacag ggcaacgttc
     2761 tgacaggaga tttctctgtc aacgtgcacc taggtttcaa ggtagaacat ggcaagatag
     2821 tgggccgcgt aaaggacacg atggtcgccg gcaacaccat aaaagcactt aacgacatag
     2881 ttgctataag cgaccgcgca gagtgggcct atggacagta ctccgcccct gcgttcttgt
     2941 tccggtctct tggaatagcg gctaaaggaa gttagggggc cacaagatgg aatgggcacg
     3001 gcatgaatca gaagcgaacg ccttgcccac gccatgtgct catcgggtgt acccggatca
     3061 ttcggcggcc aacggaaacc gctaatcgcg ggttcgccag ccgactgacc cacccatcgg
     3121 tcgaaatctc ggggttacct cgcccttgag cgcatcatac gaaaggtgat gtaaaagcag
     3181 cgccaattat acgcccatct acaagagcca ccgccctgaa agaggtttgg gtcagcatga
     3241 ggtcaaaccc gccccgctga cccaccaatc aacgcccggc tcgcccatac acctgctcca
     3301 gcagcccttc cagctccagt atgtcgcgga taacccaatc ggctgcatat tccgggccgc
     3361 ccctaatccg cctggtgcca tctgaatcca ccagaacggt gtggattcca aacactctct
     3421 tggcaggggc caggttccgc aaattgtcct caacaaggat gcactcggct ccctcggcgt
     3481 ccagatagtc aaggatccgt ctatatgctt cgagttcagg ctttggacag caacccgccg
     3541 cctcgtaatc gaatatgcag ttaaaatgcc cgggggttac tctgagggct gaaaggaccc
     3601 tctgagcatg gctgcggtct gcattggtga atatgacctt tgccgggctc agccggctta
     3661 acattgcatc cagttccgga ttgggatcta tgtggtcaga atgaggcaca tcgtgcacga
     3721 attccatgta gtctcgcacg tcgaccccgt gttcgtgata tagccctccc agggtagctc
     3781 cgtattccat aaaatacctc aaccggtatt cctcggcctc ccgccgagag acaccgaggc
     3841 gaagcattat gtactcgatt attctgtcgc gcacgatgtt catcagccca ttggacgccg
     3901 ggtatagtgt ctcgtctaga tcgaacagta taaatcgaat gggcacaacc actcacccca
     3961 agcaaggata tcactgcagg ttaagcctgc gtgggcaggc agttttcgag attcacacga
     4021 tgccattgtc aagacgataa tggcattggc tcgcaaaaca gagatagaga gacgcgccgc
     4081 ggtcggggaa cctgcatcct cccgttccgc agcgcgctga ggaaaatcga tagatgatac
     4141 gggcacaaaa gccaatctaa gagcaacata cgcctctcca gacttacctc gagagatggg
     4201 tgcttaagcg agtgcccatc gcagctatcc atcacgtttc tttatgtacc gctccagctt
     4261 gcgtttgact ctttgaagcg cattgtcaac agactttaca tgacggttca gttcgatcgc
     4321 tatttcctga tatgatttgc catcaaggta tgcgttgagc acgtccctct ccaggtcgct
     4381 aaggaactca tccatcttgt cctcgatatc gttaaactct tctctgctga taacgagttc
     4441 ttccgggtca gtaatcttgt tgcccgacaa aacatccaac agtgtacgat ctgattcttc
     4501 atcatatatc ggtttattca gggaaacata tgagtttagt ggaatatgtt tctgtctcgt
     4561 cgctgtctta atagcagtaa taatctgtct agtaatgcac agttcggcaa aagcacgaaa
     4621 tgaagctagt ttgtcaggct tgaagtctcg gatggccttg taaaggccta tcataccctc
     4681 ctgaattatg tcctccctgt ctgcgccaat caaaaaatag gaccgggcct tcgccctaac
     4741 aaagttcttg tacttgtgga tgagatattc ctcggcgtcg ctgtcgccct gctttgccaa
     4801 ttctacgatt tcctcatcag ccagctcatc atagtgcaaa tagatttcat attgcaggtt
     4861 tacgctcaaa tgcgtcccct cccgacacaa caagcttagc aacgggatat cttgataccc
     4921 gagactggct gtgaacaatc aactaccggc taataaacaa gccggccact ttgacaagag
     4981 tttagaaaag cccacttcgg caggtattaa gcctgtacca gctccaaaga ctacggccat
     5041 tatatatcag cgacttcaca cgagtcaagc aaagcgcctg gtagccgctt acatatacaa
     5101 tagctatctt tggcaatcac caccttcaac ccacattcca accccatttg ccatgtacag
     5161 ccgatggtgc caactgcggt ctgcagcaga cgtcatcaag gcagcctctc ttgccacaaa
     5221 gcacagccct atctggctct cagcctctgt tgaccccgag tctctgcctg gcaacctcaa
     5281 acgctatcac tgcccatgca actccggcat taagggaaga cacctcaccc gccatcggta
     5341 ttgagactgt gaaatcaact gactcttcta caagtctcga catgcccttt ccctcgctgc
     5401 caacaacgac tgctataggg cctgtaaggt ctgcctcaaa aaagcgacga tccccgtccg
     5461 cagcagtgcc aacaacccag actcccatct tcttaagcct gtcgatggct tgtgagaggt
     5521 tgcccacacg ggcgacaggg gtataagcaa ctgcgccagc agaggctttc gccacaacag
     5581 ggctcacctg agaactcctt ctagccggta tcaccactcc atgtgcgccc actgcattgg
     5641 cagtgcgaat aatggatccg agattctgcg gatcggtgat cccgtccagc accgccacga
     5701 acaagtcctc tctgcgcgct tgtgccgctg ccgcgaggtc atcaagctct gcatagagta
     5761 ttggcgaaag ctgcgccaca actccctgat gaactactgg aacacccgtc tccgacgctg
     5821 tcattcgatc gagcgccgcc ttgtccactt ccaccacagg aattctccgc tcccgcgccg
     5881 ccgcaactat tcgcttatcg tgtccgcctc gagcgacaaa tatcttgttg aactctctat
     5941 ccgactccag cgcttccatt acaggctggc gtccccatat gatgaactca tcatccattg
     6001 gctggttcct cgactccatc cttttgttga gccaatctca tggcttcctc catgatttcg
     6061 cgcagccttt cgtccttacc tgcgagatac atgtatccaa gcaaggcttc gaacccggtt
     6121 gcataccgat agtctatagg atcagggccc cgaaaaacat gcccgggttt cacatttctg
     6181 ccccgtctca ccacgtctct ctcttctgga gagaggaagt ccactagctt gtggacgatc
     6241 tgcgactgcg cctcggcgtt gaccatctct tctgccgaac gatgaagagc gttcacgcga
     6301 atgcacgctc cgccctcagc tgcaagctgc gaacggacaa aaagctccca aacggcgtcc
     6361 ccaacataag ccagattaag cagggacatc tcacgcactt gaccctcatt tagatgcaaa
     6421 cttccacctc gccccgtctc ttgtatcctc gacaattatg tccatctcag cgagacgatc
     6481 acgaatcatg tccgatacgc catactcctt agcctcgcgc gctctcgccc tcacatcaag
     6541 aagaagctcc accaaacctt ccacgatttt ctccgcattt gccagctctc cttcatcttc
     6601 cttcgcattg cccgctgcag gcttcatgga tgagggatct attatgccaa gaacatcgcc
     6661 caaatccatc agagccaaca ccgctcctga gatggccacc cgggcttctt cattgggagg
     6721 cgatggcatc tccctgttca cataagtatt tagagcagtt gcaagatcgt gtagcgatcc
     6781 tattgcgcct gcggtattga aatcgtcgtc catcgactcc tcgaatcttc ttcgaacttc
     6841 tttcacaact ccatccaatc gggcttcagc gctgtcatcc gcccaagcct tgcctgattt
     6901 tgccgccgcc agtcgcacca tttcttcccg attatccaga tcaattgacg ctgtaaatgc
     6961 cgcgttgaca atcgcatctt ccaatcgcga aagtgccctt tcgcacatgc gcagctcatc
     7021 gtcgctgaaa ttgatcggct tccggtagtg attggatatg aggaaatagc gcaccacctt
     7081 gccggggtat tgctggagaa tatcccgaac ggtcttgaag ttgccaagcg atttagccat
     7141 cttctggccg tctacattga tgtagccatt gtgaacccaa taccgcactg gcgccactcc
     7201 gtttgcgcca tggctctgag ctatctcgtt ttcatgatgg ggaaagacca gatcaacacc
     7261 gcctgtatgt atatcaaatg tcttgcccag atatttcgta gacattgcgc tgcattcgat
     7321 gtgccatccc ggccggccgg gcccccacgg actatcccac gagggttctc ccggctttgc
     7381 cgccttccag agagcaaagt cgagcgggtc ctgtttggcc tcgccaacat cgactctcgc
     7441 acctgccacc aaatcatcta tattctgatt cgacagcttt ccatatggct caaacctgga
     7501 gacatcgtag taaacatcgc catctccaac ataggccaaa cccttttcga caaggatttt
     7561 tatcatgtca atcatatcgc caacatgttc tgtggcccta ggctgaatat caggagcctg
     7621 aacaccgagt gcttctctat cctctatgta ggcagtggta aactcgtcga caatatcctg
     7681 gggagccaca cctctttcag cagccctctt gatgatcttg tcttctatgt cggttatgtt
     7741 ctggaccagc gttacattat accccctgta gcggaggtat ctccgaatga catcaggcac
     7801 cacaaaggcc cgcgcattac caatgtgaaa atagtcgtat actgtaggcc cgcagctata
     7861 gatgcccacc ttgcccggga caacaggcac gaactcctct ttacgccgtg ttaaggtatt
     7921 atagattcta atcgccatgg tccagccccc ctccaaagtg catggcataa cttttgccag
     7981 atccgccgag tcaactcgac gcagggctga gcgacgcaac agccagggcc gcgatacctt
     8041 cgcccgaccc tatatacccc agcccctcag tggtctttcc ctttatatta acatactcaa
     8101 caggcacatt catcgcatac gccaagctct cacgcatctg cggcacaaaa ggtgctatct
     8161 tcggacgttg agcgattatt gtggcatcaa cccccaagac gcgatagccc gcatcggccg
     8221 ccaacgcaac aacgcgctcc aaaagcgcaa cgctgcatat tcctgcgtat tcttgatcag
     8281 tatcgggaaa gtgcattccg atgtcgccaa ggcttgccgc ccccaaaatc gcgtcacata
     8341 tcgcgtgggt caacacgtct gcatctgagt gcccatccag tcccttctca tacggcacct
     8401 gtacccctcc aaggatcaga tcccgcccat gcacaagtct atggacatcg tatccctgcc
     8461 ccactctgac gacgctcaca acgctctcac ctcggcaatc cgagtcctcc cgcgcaagca
     8521 gaacctctgc gagcgccaga tcctccggcg tagtaatctt tatgttgtcg tacgatccgg
     8581 gcgtcagcgc aaccggaagc cccaaccttt caacgagcga acaatcatcg gtgccaacat
     8641 agccttcttc ctctgcacgc ctgtgagcgc acgccaagat cgatagatgg aatgcctgtg
     8701 gcgtttgaac aaggcacagc ccggatctat cgggagtcat ggcaacaagc ccattatctc
     8761 ccacgagctt tattgtgtct ttaggggcca cagcacaggt ggcagcgcca tgaaccgctg
     8821 ccgcctccaa aaccgcaacg atctgagctt tcgacacgaa tggccggact ccgtcgtgga
     8881 ccaatactat gtggcaggtt tcagcgtcga ccccagcctc ctcaacgccc gcacctctgc
     8941 cctcagtccc agttgccgaa gaggcaaggt cctgctccat cgctctaagg gccaacgaaa
     9001 ccgattgctg ccgatcggag ccacctgcta ccaccttcaa gcggcataac gaagagaggg
     9061 agtatcgctc tacgatatcc ctctcaaaaa cctcctcatc cccgggagga acagacacga
     9121 ttatctcgtc aacagccccg gaacgcgcca atcgtgatac tgtgtgagcc actatgggaa
     9181 tgcccctgag ctgtaaatat tgttttggaa ctgccaggcc catccgcgtg cccgatccag
     9241 ccgccggcac tatggcggta acgcgcagtc gcgctcccat tctggctaca cctcgcaaga
     9301 gaatgatctt gttgagcgaa accagctaat ctcagctgac tcgctcggcc gatttcatct
     9361 tggcaaatat catcctgcct gctgatgttt ggagcacact cgtgacaagc acgtcgacgt
     9421 aatcaccgat atagcgcctg cctccgtcta ccacgaccat cgtgccgtcg tccagatacg
     9481 ctacaccttg gccagattct tttccgtcct tgattacgtg caccgtcatt tcctcaccag
     9541 gcagtacaac cggcttgagg gcgttcgcca gctcattgat gttgagcact tccacaccgt
     9601 gaagttccgc caccttgtta aggttgaagt cattggtcag aatagtggcg tccatggctt
     9661 gccccaacct gatgagttta gaatcgactt ccgggtcatc gaatgtctcg tctacgatct
     9721 ggacagtcac attctgaccc ttttgcatcc ggttaaggat atcgaggccc ctgcggccgc
     9781 gatttctttt gagcacatct gacgaatccg cgatatgctg caactcttca agcacgaata
     9841 cgggaacaat cagcgtccct tcaatgaagc cgctgtccgc tatgtcggct atcctgccat
     9901 ctattattac gctggtgtcg aggatcttcg gcgttgcttt gcctacatat gccctggcga
     9961 tgccgcgatc ccgagctgga gcgctaacgt ggacattttc gccgctgcgt ggcaaaggcg
    10021 caagcatcgg gatgatctct tcacgcttgt gataccctac aatcagtcct gacaccccaa
    10081 agaagacgtt gagggcaata ccaatatagc ttccaaactc aggtatggat gcaatcggaa
    10141 tcgcaagcaa gttagctatg ataaggccca ctatcattcc gacagcaccc gaaataaccc
    10201 cgtgcgtaga cacttttcgg atcctggcat caacatatct gccgaaccat aacccgaatc
    10261 tagaaatcac cggcgccact gcaaaaccaa caagcccgcc gccagtcatc gccccgacca
    10321 ttactcgcaa ttccccgcct tccactgaca gggcagcccc agtctgcggc attgcgcggt
    10381 aaatgaggta accgaaaata acgcctgccc caagcatgag cactcgcgtc atcttgttgc
    10441 gcactgacac actcacctcc tcgcatttgg agggggctgt tccctccatt atagtctagt
    10501 ctcccctggg ttcccgggga gtatgtggag ctcctgttag ctaagtatct cgtcgataac
    10561 gttcgccact tcttccaagg gcctcttttc caccatggcg agctcgctcg ccaggatctg
    10621 ctttgcatta tccagcatac ggcgttcgcc tgtggaaagc cccttgtccc tgtcgcgtat
    10681 tccaagattg cggacaacct cggcaacgat gaagatatcc ccagtcttca tcttctccat
    10741 gttcgccctg tatcttcgat tccaattctg gctcatcggc gtcgcttcag ccttaagcac
    10801 ctcataaacc ttgggaacct cgtcacaatc tataaccggc ctgaggccta cttcatctgc
    10861 gctgccgcga ggcaccatca ccttcatgtc gcccatctgc aagcgcatga tgtagtaata
    10921 gtccttctcc ccgaggactt ccttctcctc aatagcctcg atcaccccag caccatgcat
    10981 agggtagacc accttgtcac caacactgta cacctcttgc atcccttctt cctcggagcg
    11041 ccttctttga attgtaccat tgcctggcaa agcagtcaat tccgccgcat agatccctca
    11101 accaccagcc atccccgtga aaaaggcaca gccctttgca gacaaataat ccaaccacac
    11161 ggagaagcgg attgacaata caattgccca aacgtataat caagatgaat tttcagcggc
    11221 catatcacct gtgttttccg cttttggagg tgaatagcct tggccgacct atcatgcggc
    11281 gagtttcaag agacggtctc cgaatatctt gtccgtcata gaagcgtcct cgacgtaatg
    11341 tcaaaacttc aggaagccgg ggccagagtc aaccgtgcag tcgcaaaatc ggtgacttcc
    11401 tgtggttgtc tggaagtttc cgcacagaag cagaaatttc cggagaatgc gaccctggcg
    11461 gacctgaaac gcatgatcaa ctcgcacatc agcggcgagc tctgcccaga atgcagggaa
    11521 gcccttgaaa ccgaattagg acaaatgtta ttctacattg cagccctatg cgccataaca
    11581 ggacttgatc ttgacgaaat aatgatgaaa gaacacgacc gcttgcgcat gctgggcctg
    11641 ttcaatctga cgtaattgag ggggagcggt atcgctgccc cgtcgcctat tcgcagggca
    11701 gattttccgc atctagataa ccaaatcacg cactacctga tcgcggagac gggctagtcc
    11761 ttcccgtact gccctcgctc gcacttcgcc gatcccttcg atctcatcaa gttcctcgac
    11821 ggtggcgttc cttatggctt tcagcgatcc aaacccttca actacgtttc cgatgacaga
    11881 cagtggcaac cgagatatct tcgacaacgc ccggtacccc cttggcgaca tcgatgactc
    11941 aggagttgca gacggatttt gagtcagggc cctcgacaag gtggccagat ccaaaacttc
    12001 gtctactgcc caaccggcaa tctcattcat cagcttttcg gcctcaacct gccctccatc
    12061 gggcatgtag tccatagcta cgaaaagaac ctcctcagcc attccgctca tgacctcgtc
    12121 agcctggatt ttcaccaatc tgccctcagt gccaagttcg gcaatatagc cagatacctc
    12181 ttcgccaacc cgcctcataa gctcgtatct ctgaactact ctggcaacgt caaccaatgt
    12241 gacttcctct atgaattcga gagcagtcag gttcgtgagc gccttgacca atgaatcacg
    12301 gtagttggcc atagtctgca atgcctgatt ggccttggcc agcacaacac ctatatcccg
    12361 cataacatat ctggtgtgtc cgtagtagac cgtaatcacc gaccgccttt gggaaatggc
    12421 gatgactaac tgcccagtca ttttcgatac ccgctccgcc gtgctgtggc gaaggcccgt
    12481 ttccctcgat ggtatgtccg gatccggagt gagggtggca ttggcgtaca tgatccgttc
    12541 ggcgtcttcc gaaaggatta tagccccatc catcttggcc aattcataca cggccgccgg
    12601 ggtaaactcc gcatcaagcc taaagccccc attaactagc ttcatcatct caggcgtatc
    12661 gcctacaaca attagccccc cagtcctcgc acgaaggatg ttctccagtc cgtcatatag
    12721 agccgttccg ggcgccacca tctgaaatat cttgcgcatt ctctccgaca tcagccatcc
    12781 accaccgcaa ataccgcctc caatgcctct ctaagagtgg aagcccctac tatctccacc
    12841 ccggatcctg ccaattgccg tctggtcatg ctcttcacat tatggctcgg aagaacgcat
    12901 cgtttgaagc cgagcctctt ggcctcattg actctcatgt cagcctggct aacgccgcgc
    12961 aactctccgg ccaatccgac ttcgccgaag accgctgtct ttggagctgc agggatgttc
    13021 ctaaagctcg acacgatggc ggccgcaatt ccaagatctg cggcaggctc tgtcagcgtg
    13081 actcctcctg ccacatttac gtagatatcc tccgtgccta tggcgaatcc cgcacgcctg
    13141 tcaagcaccg cgatgatgat cgcagtcctg ttgtaatcga cacccgtagt agtcctgcgc
    13201 ggcgttcctg taacagagcg cgccaccaat gcttggactt cgacgagcaa cggacgtgtc
    13261 ccttcaacgc tggcaacgac gacagagccg gccgcacccc ctggccgctc cgacaacagt
    13321 gcctcagagg gattctcgac ctccgctagc ccccggtctg tcataacaaa tatccccact
    13381 tcattggtgg aaccatatct gttcttggca gctcgaatta tcctgtgagc atggttgcct
    13441 tcaccttcaa aatacaagac cgtgtcgaca acgtgttcga gcgccttagg acctgcaatg
    13501 gctccggatt tggtcacatg gcccactaga aatacaggga cccctctttc cttggcagcc
    13561 cgcatcagcc ggttcgtgca ttcgcgcacc tgccctatgc ttccgggaga cgagccaagc
    13621 tccacatctt ccatggtctg aatcgaatct atcacaacga gaaccgggtc gatcgtgacc
    13681 attgcttcca ctaccgcgcg cacatcagtc tccgccagca ccaaaagcga atctgacccg
    13741 gcaccgagcc ttgcggctct aaggccaacc tgctcaactg attcttcgcc cgtcacataa
    13801 agaacgacac ctttggacgc agctatgctc tgggccgcct gaagcagtat ggtggacttg
    13861 ccgatcccgg ggtctccgcc gataagcacc accgaaccag ctaccacacc cccgccgagc
    13921 acgcggtcga attcgccaat ccctgtggaa aggcgctttt cgccgctagc agatatctgt
    13981 cctataggaa ccggcgcggc aaccgcgccc gatcctcccc acccggcccc tacgctcgat
    14041 atgggccgcg ataccctaac atcgggtcgg cgctcctcga cgaaactgtt ccagctgtca
    14101 cacgccgggc acttgcccat ccatctcgca gactcatagc cgcattcctg gcatacgaac
    14161 gccactttcg cgcccttcat acagactcct ctttctcaaa acatcgaggc tcgcgaggtt
    14221 cactcccgct gtagctccaa tgccttaaag acgagcttcc cttcggcctc gttcacctct
    14281 acgctctgcc catcccttat gtttccttct aggatctcat ctgacagtcg gtcctccacc
    14341 atcctctgaa tcactcgtct gagcggcctt gcgccatagt ctggattata gccctcttca
    14401 accaggagcg actttgcccc atcagtgaaa gtcaggtgta tgccccttgg ctccagatgg
    14461 gctttgaggt ctcccaacat cagatcgacg atctcctcca tgtccttgcg ccccaacgcg
    14521 tggaagacta tgacctcatc aacgcggttg agaaactcag gcctgaacgt gcgtttcagc
    14581 tcggaaagaa ccttgtcgcg cattcgttcg taggaattct tctcgtcctc ttccgttctg
    14641 aatcccaaca ccgtatcgcg gtgtatctgg gtcgctccta catttgacgt cattatcagc
    14701 acagtgttcc tgaaatcgac tgtcctgccc tgtccatcgg tgagccttcc gtcttcgagc
    14761 acctgaagaa ggatgttgaa gacttccggg tgggcctttt ctatctcgtc aaacagcacc
    14821 actgaatacg gacgcctccg cactttctca gtgagctgcc ccgcctcttc atacccgaca
    14881 tatcccggag gggcgcctat gagccgcgac acagtatgcc tttccatata ctcagacata
    14941 tcaaggcgta tcatggcatc ttcgtctcca aatagcgcct cagccaacgc ccgggccagt
    15001 tcggtttttc cgacacctgt aggccccaaa aagaggaaag accctacggg acgccgcgga
    15061 tccttcagcc ctgcatgggc acgcctgact gccctggcca ccgcggtgac tgcttcatct
    15121 tgagatatca cccgcttatg gaggatatcc tccagcttca gcaggcgctc ggcctcgctc
    15181 tcctcgagct gcattactgg aatgcctgtc catgaagcca cgatctctgc aatctggttt
    15241 tccgtaacca cggcaccctc caggtatctt tgattctgcc actgcttgcg ctgctcctct
    15301 atggtgtctc ggatactctg ctcatggtca cgcagctcgg ccgctttctc gaattcctcg
    15361 ttctgtatgg ctgcctcctt ctccgtcctg actcgctcga ggtcatcttc gagcctcttg
    15421 atgtcgggag gagctacatg cgctgccaga cgaaccatcg atccagcctc gtccaccaga
    15481 tctatagctt tgtcgggcag aaaccggtcg gtgatgtacc tgtcagacaa gaccgccgcc
    15541 gccctcaaag ccccgtctgt tatcttcacc ctgtggtgag cctcatacct atcacgaagg
    15601 ccgtcgagga tttcaactgt ctcctccaca ctgggctcgc ccacctggat cggctgaaat
    15661 ctccgctcga gcgcagcatc tttttcgatg tattttctgt actcatcaat cgttgtcgcc
    15721 ccaacgcact gcatctcgcc acgggcaagc gcaggcttta gaatgttggc ggcgtctata
    15781 gcaccctcag cggcacccgc tcctaccacc gtgtgcagct catctatgaa aagaatgacg
    15841 tcgcccgagg caattatctc gtctattacc ttcttgagcc tttcctcaaa ctctccacgg
    15901 tacttagttc cggcaaccaa agaacccatg tcgagctgga tcactctctt ctctttgagg
    15961 agttccggaa catcgccccg gttcagtttg agcgcaagcc cttcaactat ggctgtcttg
    16021 cctacccctg gatcccctat gaggaccgga ttattcttcg ttctgcgaga aaggacctga
    16081 ataaccctgg aaatctcggt ttcacgcccg atgacggggt caagcttgcc ttcgtttgcc
    16141 attgcagtca ggtcgcgccc atactgctcg agcgtaggtg tccctttcct tctctgaagc
    16201 ttggcccctg cctgcccctg agcttcctgc cgttgggacg atccagagac tcccccacca
    16261 agcaagtcga gaatctctgc tctggcacgg tccaaatcga ccccgaggtt ctccaagacc
    16321 tgtgcggcga cgccttcgcc ctccctgagg aggccgagta gaaggtgctc agttcctaca
    16381 taaccgtggc ccatcagcct ggcttcatct atggaaagct ccaggacgcg ctttgccctc
    16441 gggctgagcc caaccatctc tgcctggcca ggctcgccgc ggcctataat gctctcaact
    16501 tcatctcgaa ccgcttccgg ctctaccccc atgttatgca gagcgcgccc ggccacgccc
    16561 tggctttcac aagtcaacgc caggagaatg tgctcggtgc ccacaaagct gtggccgagt
    16621 ttgcgagctt cctcttgtga caggtagagc actctttgcg ctctctctgt aaaagatccg
    16681 aacatctaat gttcctcctt gttcatccct aaacgctctc tgataagtgt tgcccgacac
    16741 atatctcttt cccaagcagt gagctctttg cccacgaagt cctgcacatg cgcgggcatg
    16801 atatcgactg ataacaccgt aaaacaggat tgcggaaggt ccgggatgat cccgagatca
    16861 gccccgagct tcacgtcaga gatgagctta agtgcttcat ctgagctgat gatcctggca
    16921 ttggtcagca atccatagga tctccagacc ctgtcctcca gttgattcct agcgtccgac
    16981 attatagcct ttctggcgtt gcgctcgctg tcagcaagct gcccgcatac agccttgagg
    17041 tgcgcaacta tctcctcttc cgctcgaccc atcgtaacct ggttgctaat ctggaacatg
    17101 ttcccgatag cttcggtgcc ttcaccgtag gcgcctctca ccgcaacccc gaccttagat
    17161 accgctccca atacctgccc caccatgccc accaaagcaa gggcaggcaa atgcaccatt
    17221 accgaagccc gtaaccctgt cccgacatta gtgggacaag cacacaaata tcccagattc
    17281 tcgtcgaacg cgtaatcgat cgtctcctcc accgcgtcgt cagtaaccga agctaacctg
    17341 agagcctctt caagctggaa cccgggcaat atcgtctgta tcctcatgtg gtcttcttcg
    17401 ttcaccatta tgctaaccga tttgtcccga ttgaggacga tcgccttgtt ttttgggtcc
    17461 tgtatatgtt gcggcgaagt caaatgctcc tccaccgcaa gctgtctttc tataggaagc
    17521 agttcctgta aggccagtat ctcagtgggc ccaatggacg gagttgccct gacagccccc
    17581 attacctgct caagaacgga tttcatctgc tcatcattgg cctgctgtgg aaagggtacg
    17641 ccgcgcagat tccgcgcgag cctcacccgg ctagatacca cgatatccgc atctggacca
    17701 tccccggcca tccatactgc gcgattcttc tcatacggtc tcttcgccgc catccccact
    17761 gcctcccttt tccttagctt ccgcgtcgcc gcctgtgcca tcgatctcga cctcagccgc
    17821 agccttctcc agcctgcgaa tctcgtcgcg gactatggcc gccttctcgt attcttccgc
    17881 ctcaacgtac tgctgcagcc tcctgcggag gtcatctatt tctctgcgaa aacgcacagc
    17941 cttccctgct cttctgggcg cctttcctgt gtggcgtaca tgtccgtgaa tgcgtcccaa
    18001 aagagggtta agcagcggcg caaactcaga atagcaatcc gcacacccaa gaaacccgct
    18061 attcacgaac tcagaataat cgcgcccaca cgttctgcac tttctgccgg cgccatcggg
    18121 aatcccaaca tgcacctcgt ggcccaggtc accctgaaga agcccggcga gtaggttgga
    18181 aatagcaaaa ctgggcgtgc tcagcacgtg cagttcccca cgctccctgg cacattgctg
    18241 gcagaggtgg agttcagtgc ggacattatt cacgaccttt gttacatgca cagtcgctgg
    18301 tttctcacga cacacctggc aaatcattgc ttaccctccc tgttctactc catcggcggc
    18361 cgtgtcagca tcagcataag cacggcacgg agcacccgcg cccgtatgat cccctcaagt
    18421 tcagggtctt catcgctttc ctgatcgaag actgcggctc gaacacaggc cgcgtctttg
    18481 caatcgaggg cgcccatatc aacaagccta gcgatcaggt cttccgctgc cttacggtca
    18541 atagcatctc ctacggactc gtacaccatc tggatgccac tcgtttcagg ttcgtcgtca
    18601 agcttgaaga ttctgatgta gccgcctccg ccgcgtctgg tttctacaac atatccgcgc
    18661 tcaacggtaa agcgtgtgga caacacgtaa ttgatctgcg acggagcgca cctgaatctc
    18721 tcagcaagct cccgtctttg cacttccaca aagccatgag ggcccgtgtc aagcagcagt
    18781 tttaaataac tctcaatgta atccgataga ctgcccatcg cgtcctcccg ttgatctgac
    18841 cattgttgac cttttagtac catgatacac ttgtctgtcc aaaagagcaa catgcctaaa
    18901 ggcctcttgt agctatacaa accggcaact gaccaatttg gtcaaacaag gtcagacgcg
    18961 tccaggcaca tgatgtggga tgtcctgaca ggctatttcc tttgaattgg catggtgaaa
    19021 ttgccaattg ctaggctggg gaagcaatct ctgatatggg cgatggcttc atccactccc
    19081 gcaggataaa acgaatcgga atcgacattt tccgtgagca tgacgtcggc gtagatgtct
    19141 gggtcgatgt tgaggtttcg aagctggagc atctggatat cacaacgttc tatgagatcg
    19201 cataacgcgt caacctcggg ttcctggtca aaaaacccag gaaataccag gtagttgagc
    19261 gatacgaaca aactgcggct tcgggcagca ataatcgagc tttcaacatc tgcaaggcac
    19321 caaccacgag gcctatggta tctgccataa taatcagggc gggcactggc aaggctcacg
    19381 cggagcgagt caacccctgc atcacatatc gcctcgatgg cgtgcacatc accggcattc
    19441 gtattcacat tgatcgtacc cgcatccgtc cgctgacgca attcccggca tgcctcgacg
    19501 atcgtcggcc actggcgagt gggctcgccc tcacatccct ggccaaagct gattatcccc
    19561 ccgacagctt tctccagatg gggcaacgcc acttcaacaa tctcagagac tgtggggacg
    19621 aaatctatcc gttgctgagg ggagggacag cactctgacg cctggagtga tatgcaccct
    19681 aggcaattgg cattacacgt gggcgagaca gggatgccgc cttcccaacg cccgtagaat
    19741 acgttctggg cagtgaagca ttggtattct gttgcacatc tggcgagctg cgcaagtatg
    19801 cggttttcgg ggaattgtct cagtcgcctc tcaatatcct ctgcaagctc tggaaggttg
    19861 taatggatcg gatcccacgt gtctcgctca tcagtcctga ttgcagctac catcaactga
    19921 tcaccggcaa aagccacagc agaataaccg aagagcggaa gttgtggcgc cccctccaat
    19981 cctatgtatg cgggaagaaa cgtgcgagtg tagccctgag gaaggactgc tgctaccgcc
    20041 tcttcaagtc cagcactgac gaattggcct gatgtcgcat caatgcctac aggcctccga
    20101 ctaggaaggc gcatcaaact tccgccttcc ggcatcggga ttacgtcaga ctctaacagc
    20161 tccacgaggg tattgcctgt gcgcgccgcc gcatgcagat tatcatttct gaatactcgg
    20221 ccttggtgat cgctgactgc tgtagataac atagaacatc tcctgtttca acatggcgct
    20281 tttagggcag ataacccatt ggatcctgag gcttgccgcc cgccctcacc tcaaaatgaa
    20341 cgtgaggacc agtggaaatc cccgaactcc cggacaaggc gatcctttca ccggctgcaa
    20401 cctgttcccc gcgggacacg gtgatagctg agttgtgtcc gtagtatgtt tgcaagccat
    20461 ttccatggtc caggatgacg agccgtccgt agccgcctct ccagcccgca aagatgacac
    20521 agcctgcagc agccgccgat acagatgtgc cggtcggcac agcaatgtcg acgccattat
    20581 gcatgcggcc ccatctaggt ccgaacctcg atgtaatcct tcccgaaacc ggccaggaga
    20641 ggcttccgcc cgatgatgcc gcaccggaga ttcttccatc agatgaccct ctcgaggcta
    20701 ccacgatgcg actactagtc actgggggtt tgcccccggg aataacgagg gattgcttca
    20761 ctttcagggc atcagggtcc gcaatggcgt tagcctcgac aatcttatcc cggctgacgc
    20821 cgtacctctt ggcaatagtc catacggtat cgccgcggct aacctcgtgt atcacgcctg
    20881 taactgaagg gaaagtaagg acctgtccga ccttgagttt gttgggattg gccaacgaat
    20941 tggcccaact tatcgtctct atgctcactc catagctgtt tgcgatggtc cataagcttt
    21001 caccctgctc cacccggtgt gtaacaatgc ttgcttgagc ccgagcaaac gatgaagagc
    21061 taccttgttt cccagcgtcc aagtccaggg cattcgtcgc tgcaacgctc gggcctatga
    21121 gctcgctctc tgttttcagg gtggatatta tattggcacc agggtccaga cggggcttcc
    21181 tcggatacaa tccgctttcc cagtcagcaa ccacatcgga cttggctcca agtgccgcta
    21241 gaagtatcgt ccagctgcgg ctgaatatgg cttttacaag ttccaaagca ccggatctgc
    21301 gcattacatc ggtgctgaag ctcacctcat cagaacccgc cgccatagcg ccacaagaaa
    21361 atgttggcac gcaaaaagag ataacgagca cgcacataaa cgcagctaat ccttgttttg
    21421 gcaaccgtct ccctccaata agcctgactc caaagcctcg tggttgatgg ccatactaca
    21481 tattataacc acatgcgaga agaaggtcat ccctaagcgt cgactgcctg tccacgccga
    21541 tatttccgga cacatgagaa gcccaagaaa atgaaacgag gggctcatca agagctcact
    21601 gctccagacg aggaactctc agatactcct tgactttgat tgcgggatgc gcctcacatt
    21661 gaattgtgcc agtttcgcac attctcggcg aactgggatt tgcggattat gttgatcaac
    21721 tcttcgttgc tctttgtgcg ctgaatcctg tcaataagca gttgagtcat ctccgtggaa
    21781 ccagttgtgg ccatggcctt tctcaacaac cacatggtct ccaactcttc cgcgtccatc
    21841 agaagctcct cttttctcgt tcccgacttg ttaatgtcaa tagctggaaa gatcctcttt
    21901 tccgccaact tccggtcgag atgcaactcc atgttgccag tgcccttaaa ctcctcgaag
    21961 ataacctcgt ccattctgct gccagtgtcc actagcgcag tggctatgat tgtaaggctg
    22021 ccgccttctt cgatgtttct cgccgcaccg aagaacctct tggggtgatg cagagcagcc
    22081 ggatcaacgc ctcccgagag tgttcgtcct gacggcggtt cctcgaggtt gtatgcccgt
    22141 gccaaccgcg tgatggaatc gagcaacacc aaaacgtctt tcccgtgctc cgtgaggcgc
    22201 ttggcgcgct ccagcaccat atcggcaacg cgcacatggt tcgaagaggg ctgatcgaaa
    22261 gttgaggcca caaccatgcc gtcaactgaa cgttccatat ccgtgacttc ttcgggtcgt
    22321 tcgtcgatga gcagaacgag gagggtcaat tcagggtggt tatacgtcag agcgtttgct
    22381 atcttcttaa gtatcgtggt tttaccagct ttaggcgccg acacgataag gcctctttgc
    22441 cccttgccga tgggcgccat gaggtctatg aggcgcgtcg tcacatcctt aggatcatgt
    22501 tccagcttca gtcgttcatc aggatagacc gggatgaggt cctcgaaatg agatctccgc
    22561 gccgctaatt cgggatcgag cccgtttacg gcctcaacgc ggagaagcgc aaaatacttc
    22621 tcgccgtcct tcggaggacg gacctggcct gacaccatgt cccctttccg caagccaaac
    22681 ctgcgaatct gcgaaggaga tatatagatg tcgtcagagg tgggggtcat tttccccaca
    22741 cgcagaaagc cgaagccttc tgggagcact tcgagtatcc catctgccca cagcgcgcct
    22801 tccttctgaa tggcagcttt cgttatctcg aacgtgagct cctgttttcg gagttgcgag
    22861 tgacccggaa tccccagctg ctttgcgatt tcatgaagcg caggtacggt catcttttcc
    22921 aattcagcaa tattcatctg tgttgcatct tcctttcagg atatatgcgc taaaggcaga
    22981 gccatccggc gcgcaggcta ccgccgtgcg ctaagggaac ttctattgag gctatttgat
    23041 tgggcatact attggtttta gtgatgagac ttgcttgaag cattagcttg atcgcgaagg
    23101 tcctcaacat gtcacagaga cgctattcgc cacagcaaca acaaaatcct ccggcgaagc
    23161 aacctcacta accattattt ccacagcaca ttcattccat gacgactata agctgcgctt
    23221 atctcgattt cgagtattcc atagacttga caataaattc ccgaaacagc gggtggggct
    23281 tgccaggcct cgacttgaat tcgggatggt attgcgtacc cacaaaccac ggatgatcca
    23341 tcaactcgaa agcttcgatc atgtcgccct cagggctcgt ccccgagatc ctcattccgt
    23401 attctgacaa cacctgcgca tactcccaat tcaactcgta cctttggcgg tgcctctcct
    23461 ctacaacgtt ctcaccataa gccttcgcaa gcttggaagc tgggtccaac acgcacgggt
    23521 ataccccgag cctcatggtg ccgcctatgt gctcgatctc gcgctggtca gccattagcc
    23581 gggccacagg gtgcggcgta ccagggtcaa attcagtgct attggcgccc gacagtccac
    23641 acacattccg agcaaactcg atgacggcac actgcatccc catacaaatg ccgaagaatg
    23701 gcacaccatg cttacgagca tagcctgcgg ccaagatctt cccgccgaca ccgcgaggcc
    23761 caaagcctcc tggcgcaatt atcccgtcgg caccacgaag cgcttcctcc gcgacgagct
    23821 cgctggaaat gctctctgag tccacccaat tcaggtcaac ctgcacgcca cagtcgattc
    23881 ccgcatgacg taaggcttct accacactga ggtaggcatc ggggagcgac acatacttgc
    23941 ctaccagggc aatgctgacg gagtctgaga gatttttcga tttcttcacg aattcgcgcc
    24001 aactgcacat atcggctctg ttttcagaac acggcagttc aagcctgcga atgactatcc
    24061 tgtcaaggcc ttcctgctcc aatatgagcg gaacatcgta gatcgaatca gcatcgggat
    24121 tgaagatgac tgcctccggc tcaacgtcgc agaatagggc tatcttgttc ttgatctcgg
    24181 gcggaagaat gcgatcggac cgagccacga taacgtctgg ttgtattcct atgctccgca
    24241 gttctttcac cgaatgctgg gttggcttcg tcttgaactc gcctgcacag gcaatatatg
    24301 gcacgagcgt gacgtgaatg tacatcacat tttcgcggcc cgcttcagtc ctgagctggc
    24361 gtgcagcctc cagaaaagga aggctctcaa tatcacccac agtcccgcct atttccacaa
    24421 tcacgacttc ggcgccatca cggcccagac gtgttaccct ctctttgatc tcgttggtaa
    24481 tatggggtat tacctggaca gtcccaccaa ggtagtctcc acgtctctcc ttggcaagga
    24541 ccgcagaata gatgcttccg gtagtaacat tattgtcgcg agtgacattg atgtccataa
    24601 atctttcgta gtggccaaga tcgaggtcgg actcagcgcc gtcctcggtg acaaagacct
    24661 ctccatgctg atacggactc attgtgccgg ggtctacatt gatgtaggga tcaaacttga
    24721 gggctgctac ctcgacgccg cgcgccttga gcaaccttcc aagggatgct gcagtaatac
    24781 ctttgcctag tccggatacc actccgcccg taacaaaaac atacttggcc aactagctgc
    24841 ctcctctacc ggatgtccac taaccgagtt cgtattatat cactaggatt tgactttcac
    24901 aacatggctg aacttctttg agtttttgtg ctgtgctttt tgcctcgggt ccaaccatcc
    24961 gctagcccct ctcaagccac agccagcaag gcttcggatg acgaaagcac gttctccatg
    25021 agaagcatct cgagcataat gcaagtatta caaaaggcga taacgttcaa taagtgagga
    25081 agacgctgaa aatgctgcaa gcaacatgtg tttcactgcc gataaggcaa tcacaggact
    25141 aagtagaccg caacaaatct ctgcccctcg agtttgtact gccgctaggg gactgctaag
    25201 gtcttttact tgtaaatttg gccttcggta gaagcctagg gaagcgccgt gcggcgtgga
    25261 gccggccaag gttcaccgcg ggggcgagca ggttttgcag accagtccaa tttttagaca
    25321 tttgcgatcg caaagcgttc aaaa
//
LOCUS       DAOSYL010000033        24753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1657_length_24753_cov_7.484452, whole genome shotgun sequence.
ACCESSION   DAOSYL010000033 DAOSYL010000000
VERSION     DAOSYL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24753
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1657_length_24753_cov_7.484452"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..>1700
                     /locus_tag="PLU15_06790"
     CDS             54..>1700
                     /locus_tag="PLU15_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOO30666.1"
                     /translation="MPSEADVKNLTGLSGDEAARRLAEEGPNELPTSKKWSIWAIVLE
                     VVGEPMFMLLVGCGVLYLLLGDTKEAAMLMGFVFVVMGITIYQERKTENALEALRDLS
                     SPRALVIRDGRQLRIPGREVVCGDIFLVSEGDRIPADAVLIWGINLSVNESLLTGESL
                     PVRKVTAEGRPESIDMGRPGGEETPHLYSGTLVVQGQGVALVKAIGIQTELGQIGKAL
                     QTVELEETALSRETGRMVRKLAILGFVLFAIVVLAYGATRGNWIDGILAGITLAMAML
                     PEEFPVVLTIFLALGAWRMSQKNVLTRRMPAIETLGAATVLCVDKTGTLTQNRMSVSR
                     LSVAGELYEAVGARVEVLPDKFHEIVEFAILASEKDPFDPMEKALTEFGHEHLINTEH
                     LHDDWALVEEYALSREFLALSHVWMSSHADGAFYVVAAKGAPEAIADLCHLDEAKTCG
                     LMDEVSRMAADGLRVLGVAKAEYGVGDLPERQHDFEFELLGLVGLADPIRPAVPDAIG
                     ECYQAGIRVVMITGDYPGTAQHIAREIGLRPSDVVITGSEL"
     assembly_gap    1701..1800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1801..2703
                     /locus_tag="PLU15_06795"
     CDS             <1801..2703
                     /locus_tag="PLU15_06795"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOO30667.1"
                     /translation="ELREMVKTCCIFARMVPEQKLRLVEALKVNGEVVAMTGDGVNDA
                     PALKAANIGIAMGGRGTDVAREAASLVLLDDDFSSIVRAVRQGRRIYDNLRKATAYIF
                     AIHIPIAGLSLLPALFGWPLVLLPVHVAFLELIIDPACSVVFEMEQEEADVMRRPPRD
                     PAKPLFGAELMRIGIAQGLSVMAFVTAVYIISLFLLDRGEMEARSLTFTTLVLANLGL
                     ILANRSWESTTMAGWRRPNPALKWVVGGAIVFLATVLSVPALRTLFHFTVLHPVDIAI
                     CFATGTLSIVWFEVFKALRMDVVS"
     gene            complement(2824..3702)
                     /locus_tag="PLU15_06800"
     CDS             complement(2824..3702)
                     /locus_tag="PLU15_06800"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOO30668.1"
                     /translation="MRVRISALAFTLILVLTMSASALAADDPVGTAAMMDIGMGARAL
                     GMGGAHIAVADDSAAVYYNPAGLAFMSERHVGSLYTQQYGAAGFMAAAYAQKHVGVGG
                     LRLDASGIEGTDEFANETGVFGVSDFTVIVCGGIEALPNLGIGAALKYYSQALPDNPG
                     KGVTGDIGLLFIAPSKKLRVGAVVKNVGGELKYSSGYADAFDRSVGAGVAFYPVDRLL
                     LAADLIYKDELETRIGGEFQLGRILLRAGGAFGSKQNSLTAGAGFSASMFTIDYAYQT
                     HSILPDSHRLSLGMKF"
     gene            complement(3801..13382)
                     /locus_tag="PLU15_06805"
     CDS             complement(3801..13382)
                     /locus_tag="PLU15_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO30669.1"
                     /translation="MAKSHWRKTPWIALLIMSLALALATAVGAAQSTPSGLPRGGWQP
                     GPDIYHPSRVIVRFSDVVSSEHATQSIAALGYSVDRVADFGVSANFPHGLRIGIVELP
                     DGVSPDTAVDRLSKTPGILYAEPDYIRYKDQAYGDAPIFPNDPQFNKMWGLHNQNCQF
                     KDPRMSGDPVDDADIDAPEAWGICTGSEETVVAIIDTGSYILHPDLAPNIWVNPGEIP
                     GNGIDDDGNGYIDDIWGWDWFNGDNSVWDPGERDQYGYLNDEHGTHTSGTIGAITNNA
                     IGVAGINWNIKIMILKFLGPEGGYTSDAILAFNYAANKGAKVISCSWGGGGYNQALKD
                     AIEATHAIVSCAAGNSGQNTDINPHYPSSYDSANIISVAAMMQNDTPCNYPGWWSTCW
                     GPTSVDLYAPGGYILSTIPPDPPPSQPGEAYDFFYGTSMATPHVSGAAALLHSLRPGL
                     PLYPSAPGSIPGQQTVKDILLNTVDVKPAYEGNVVSNGRLNIAAALLELGGPVITSID
                     AQPRFGPPPLEVTFTASAISPGGEIVDKWWDFGDGSTPVHEFNAVHTYTEQGDFTATF
                     HAVDGEGLEATASLTIKVFFPPVIGVDPDEIHVDLWWAEETTRQVTVSNTGLGELNYS
                     VNIRLVGKMNASGEARLGAGGPDSFGYFWLDTDEPGMEPPEWEDIRPIGTQITLEDDN
                     GVVVDLPFEFPFYGQKKSQIAICSNGYLTFGGKLNAWTNTTIPNTADPNDLLAVFWDD
                     LTLSSSGTCHYYGDEDIFIVQYTNVPRLSSGGPYTFQVRLSPQGVIMYQYLTMAGTRL
                     NEATIGIENATGTVGLEVAYNKNYVHDDMGIMFLPGWIVSSSSGGTVEPGEVDTFDLM
                     FFADHLPKGTWKAVVEIESNDPANPKVDVDTYMFVRSRINPTIRSASANPWAGSAPLE
                     AHFSVDAYDQDGEVVDAIWDFGDGSPVVSGTFNPIHTYMAEGNYTATVIVVDDDGFEA
                     AAEIDIVVTDLPQVAVDPASFNQVIRAHRQRTETLTVTNTGVATLEFAATAYTAGIPA
                     EAGELDPFGAGGPDGFGYMWRDSDEPGGPIFEWVEISGIGTKLPVSGDDSRVVNLPFD
                     FPFYGDIKTEIRICSDGYLTFGTKGTAYTNVAIPNPAEPNDMMAVYWDDLNPPQAPAD
                     GGVFHYYDAVNNRFIVQWNKVPRYYNNGQYTFQAILYPNGTIVYQYLNMQFASATYEK
                     DGTIGIENATGTDGLQVLYNTPGYMHNNLAIRIKPISWMSVEPTEGELAPGESIGLDI
                     TFDLALVGSGTLDGAVVLDTNDIRNPRTIVPVHIHVIPNSPPTITACAANPQQGPPET
                     SFQFVGAACDPDGSIIDKYWSFGDGSPVVHEFVANHIYAAAGVYTAVFTAVDNDGYTA
                     TATVTVTVAQSASASWTPKQFSFTVGGGQTATGILTLSNAGPGALLFGSGEFPSMIQT
                     PERLIAPEDIKDTEAKTAFGLYLPNQNPERSKWLPEAVGSVIKSWQCPSPIGDPWGVG
                     VLLDSDEVVIADGAVDPTDDYVVTSDGAYTGRWWSADFGGSWSGDMAFDGTYIWQVNV
                     GGDNAIYKVDPASGQTVGSIRNTAWASVSQRGLAYNANDDTFYIGGWNEDVIYKLKGE
                     SWDSPGAVIEQWSMPVSIAGLAYHPVADVLIVTCNSSPDMIYFVNATTHATIAQFQHP
                     AGGAYMGVGCELARDGNLWVASWGENKMYLVETGLGPIGSGDWLSWQPAEGSVPAGGS
                     VQITITANSEKLSPGPHEGNVVLTTNDVENPLIVVPVTLQVAEPPAITKATAEPTFGE
                     PPLEVTFHAAFVAPEVPVASYGWNFGDGISSTELDVVHTYTLPGNYTATFTVIDQLGA
                     TAEASIDIVVKHSPHATVDPALIEITLPPNGEATETVTVGNVDGNAPLTFSVKVKGGS
                     APHVAMPERIGTVIDPNAATAEGLYMPFDPAAVERIAANIQPGGVGDVVKSWPAPAAV
                     EIAWGLGFDATNVWLADPLPKNDYIITPEGVFTGTIFNTPWAGSWPGDMAYDSNRNLM
                     WQVNVGGDNGIYGLNPATGEVVTKITSGGAWTSVSQRGLGYDADTDTFYIGGWNEDII
                     YHIMGPSWSTPGAVIAAYSFPVSIAGLEYHPDGVLWVSTNSAPDMIYGVDMESLAVIH
                     QFQHPYRGDYSGAGLALNDDGNLWVASQTDNYVYLIDTEMPLSAGIVVQPTSGTVPTG
                     QTADLAVTIKAAELGKPGQDVRKYLEILTNDPVNPALFVDLIVHIEPGPTIVEVTATP
                     EIGQPPLTVAFAATVEAGAKPITDMWWEFGDGSDLVHEATTEHVYTDLGVYKAIFHVV
                     DENDVEATAEVTVTVKWLPVLGVEPEEFDAVIQVGEEKQTILTVSNTGIAPMNFEIAV
                     APSFAESPEWKAYAASEPIKGDYASEPRGYAGAGAGGPDHYGYVWIDSKNPGGPAFDW
                     VEISGLGTRVYLGDDDGVMVPLPFTFPFYGQMKTQVGISANGYLSFDPDSINYYWTNA
                     PIPTSAKPNDMLAVFWDDLYPGGGGEIYYYNDTAGERFIVEYKNVPRLGGSASYTFQV
                     ILKPNGTIIYQYLNMVGLLNSATVGIENTTGTDGLQVLYNAAYIENDLAIGFAPVGSI
                     LKVNPTSGYLVPGGYQDVVLTLGSPDAPYGTYSLNLYVSANDPYRPFTTIPVKLKLNA
                     APSVVITAPVGGAELHGTCEVEWTATDPDDDGDNLLIDLAWTRDGVEWHDLGTGLSNT
                     GAFEWNTIEVGKAGDSFRLRARVTDPTGAYHEFVTGEFTIINNAPTADFGFTPSSATR
                     RDVVKFTDKSTDDGRIVAWHWEFGDGTDSSEQNPEHRYAEKGEFTIALTVTDNGELKG
                     TVEKSIVVGNAAPIAAFSFSPESPKAGEVVTFTNESTDDAGIIAYFWEFGDDTTSVAP
                     EPTHTYATSGIFTVKLMVIDDDYTASSVEHDVEVVNAAPVAAFSFTPASPTVHDNVEF
                     IDESTDDGPIAAWHWTFGDGAESDQQNPSHRYASKGKYTVTLTVTDNGGLWGTAEGEI
                     TVVNLPPEVTIVKPEVGTVWTGMQTIEWEAVDPDDDANTLKITLEYKPAGGQDWQTIA
                     ANQANTGTCVWDTSSVKQGGRYRIKVTATDPSDATGQAMSDEFTIVVLTHAIVAAPNP
                     AKDGVTFYYDLAADATLYVYDIAGRLVYSAELPAAAHAHEWNLRSGDRPIANGVYLYI
                     AVSGQEKTEVGRLVISR"
     gene            13952..15346
                     /locus_tag="PLU15_06810"
     CDS             13952..15346
                     /locus_tag="PLU15_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011246575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOO30670.1"
                     /translation="MRIETDSLGKRQVPVDAYYGIQTLRGQENFQITGLSVHPALIRG
                     LIMVKKATALAHSKLGQIPSRIGQAIAEAADDALAGKLDSSFMLDPIQGGAGTSINMN
                     VNEVLANRAIEILGGVKGDYSLVSPLNHVNMGQSTNDAVPTGARIALLTQLEGTIEGL
                     DALACALEAKSEEFKDVVKMGRTHLQDAVPITAGQEFGAWAKALRRDIARIEAASEGL
                     HIINLGGTAVGTGLNVDIRFIHLVCEELSKISGYPLQTAEDLVDCTQNVDVFANLSSC
                     MKVCAVNLCKIANDMRLLASGPIAGLHELLLPPVQPGSSIMPAKVNPVIPEVVNQVAF
                     QIIGNDLSVTMAAQAGQLELNVFMPVLLFNLLQSADILGNAARTFADKCVVGIEVNRE
                     RCLNMVETNVGVVTALVPYLTYQVACDVAKEAQATGKTVRSIVLERGLLSEEQLDHVL
                     SFSHLTSPSGMDGA"
     gene            complement(15485..16903)
                     /gene="pyk"
                     /locus_tag="PLU15_06815"
     CDS             complement(15485..16903)
                     /gene="pyk"
                     /locus_tag="PLU15_06815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOO30671.1"
                     /translation="MRKTKIVCTIGPASDDLDTLRSLANAGMDVARLNFSHGVHEEHA
                     ERIRRIRAVAAECGKNIPIMLDTKGPELRIGAFESGSITLAPGDTFIITTQPLLGNSE
                     IVSVNYPGLPILVSQGDKILLDDGQLEMEVRSVKGPAVECEVIVGGVLSDRKRLSVPG
                     RRIDLPTLSSEDKMDLAFGIEHGIDIIAASFIRTASSVQEIKSFTESRGVSLPVIAKI
                     ESREGIENINSILRSADGLMVARGDLGVELPAEDVPIIQKQLIQKCRRAGKPVITATQ
                     MLDSMIRNPHPTRAEVSDVANAVLDGTDAVMLSGETASGKYPVESVRTMARVVERTET
                     AGHFAEAPVQRGGSAAITATDAIGHATVQIATELKAAAIITSTKSGWTARMIAKHRPK
                     TPIVAVATDEQTARILNLVWGVYPVQGHDIATTDEMISTAVAKAQEAGFVREGDTVII
                     TAGVPVGMSGTTNLIKVHEITG"
     gene            complement(17030..17572)
                     /locus_tag="PLU15_06820"
     CDS             complement(17030..17572)
                     /locus_tag="PLU15_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30672.1"
                     /translation="MRRIYNSHDNDRRRVFVTDIIDKVVKGIGTGVDIVGSKTKELWD
                     STRIKSKIGDLKADRHKVMEELGEEAYEMLRKNAFNFDRLYAKAAEITEIDKEIAEAE
                     EALANIREESDGEEKPEATEIEESAAEPEPADVSEEEAEEAEESCSDAPKCACGASIV
                     SGARYCIFCGRPVPSGSSCD"
     gene            17833..19188
                     /locus_tag="PLU15_06825"
     CDS             17833..19188
                     /locus_tag="PLU15_06825"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO30673.1"
                     /translation="MITLKEFLAREVVPALGCTEPGAVALAAARARQELGGEYIERIS
                     ADVSDSIYKNGMNVGVPGASGETGNSLAAAMGALAGRADLGLEVLRDCTPEDVAAAKR
                     MISEGRVELNCMPHVCGVYVEVTVESRTHAATCVIEGEHSNIVRVDVDGETVFSKEEA
                     APAGSTFPRDDGNVLADHANEQETDGFLEGIDYRTLLSLADDMGSDEVEYVMRGVEMN
                     MAIAEYGLTSESKSGLGAGKAIWKLMSENKIEKDLAFLIRSYSSAASDARMAGVQLPT
                     MSAAGSGNQGITAIIPVALAGESMGKSREEIARAVTVSLLSTSFVRSRTGRLTPVCGC
                     SVAAGAGAAAGIACLLGGNWMHSARAMQLVLANTAGIVCDGAKGTCALRVGAAACEAY
                     LAALIAVNDTGIDDAQGIVDITIEGTAQNVARLNAEGMKDVDRVLIEILEERVRRGDA
                     L"
     gene            19191..20096
                     /locus_tag="PLU15_06830"
     CDS             19191..20096
                     /locus_tag="PLU15_06830"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOO30674.1"
                     /translation="MRRVYAGTPMVGGISVGTVKALAPKRLPEAGAGVVRPVGAGREG
                     ADWTAALSLPMWEYAQIWAATVATASLLTTALQRKAEDELGGAAADIFEAQHAIVSDP
                     MLEASVFSRVEEGIPLPQAVSGAITEIVAGLEASDDEATRARAEDVYDVGLRFITTLA
                     GIGAASAMDDGQFEPVLVVGGSPSEEGVLVAHDLLPSELVMLNRKMMLGLVLEAGSVH
                     SSIDVIAEALGIPVIIQAAGVLSGLVDGESIIVDADNGTVILEPYEDELKEYASRILK
                     AGHQTFYQATQAIMHDVRKPPESLS"
     gene            20235..21158
                     /locus_tag="PLU15_06835"
     CDS             20235..21158
                     /locus_tag="PLU15_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOO30675.1"
                     /translation="MIDKVTRGKIFDIRRYSVHDGPGIRTAVFFKGCPARCLWCHNPE
                     SQRREQEVMYWPNKCRGCGLCASVCDVGAVRVEHGRPVTAWELCTGCGKCAEVCPARA
                     RTIVGREVSVSEVMDEIEMDRAFYDESGGGITFTGGEPLAQPAFLCELAQTCKKVELH
                     TAVDTSGIAARHVVELVAPFVDLWLYDLKIVDRFAHLFATGCSNELAISNLRWLVKEG
                     YDVTLRIPLIPGYNDDNESLGDFIEFIGSLEWKDPPQAAILPYHRLGNEKYERLGRVC
                     CVEDLDEPDSQNIDRVRRVFEAVGIDVQIGG"
     gene            21164..23521
                     /locus_tag="PLU15_06840"
     CDS             21164..23521
                     /locus_tag="PLU15_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOO30676.1"
                     /translation="MNTRVSRLRQQSLDAVPYISAERAELITEFYANDNTPSPALKRA
                     RAFRYLMEHKAICINDGELIVGERGPAPKATPTYPELCCHSLEDLDILSSREKIPFAV
                     SPETISIYEEKIIPYWRGRSMRDLIFTEMTDEWKAAYDAGIFTEFMEQRAPGHTVADD
                     KIYRKGFRQLREDIARHLASLDYLGDPEAHDKAEQLRAMDIAAEAICIFARRHARLAE
                     SMAREETDPVRASELRRIAEICSWVPENPPRDFWEALQAYWFVHLGVITELNTWDSFN
                     PGRLDQHLYPFYKKGLEDGTLTEDSARELLQCFWIKFSNQPAPPKVGVTAAESGTYTD
                     FANINCGGLTPDGRDGVNGLTYLILDVIDEMRLLQPSSNIQLSAKNPDRFLKRALRIV
                     RKGWGQPSIFNADMVVQEMLRVGKSLEDARCGGTSGCVETGAFGKEAYILTGYFNLPK
                     ILELTLYDGVDMRTGRQLGPKTGPAALFETYEELWEAYTEQLNYFIDVKIKGNNVIER
                     LYAKYMPAPFMSLLIDDCIALGRDYNDGGARYNTSYIQGVGIGTITDSLSAIRHHVFD
                     EPRVTMDQLLQALERDYEDREDLRRLLWDDTPRYGNDDDRADTIMTDVFKTFFDAVDG
                     RPNVKGGRYCINMLPTTCHVYFGSVTGATPDGRRAWQPLSEGISPVQGSDRRGPTAVF
                     KSASKMDHALTGGTLLNMKFSPKLLEDEAGINNLASLVRTYFKLGGHHVQFNVVKAET
                     LREAQADPEAHRDLIVRVAGYSDYFCDLSRALQDEIISRTEHQVM"
     gene            23545..24381
                     /locus_tag="PLU15_06845"
     CDS             23545..24381
                     /locus_tag="PLU15_06845"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOO30677.1"
                     /translation="MLVDYHVHLERAGLSRANAMRFVDRALANGVWEIAFTEHAYNFV
                     ECAPMLERPNYVKEHGHGFRLDDYISLIEDMKAEGLSVKLGIEFDYIDETIEDVADFL
                     EEGHPWDLVIGSVHWIGEWGFDIDPTSWNDVDVEWAYSAYFERVCRAARSGVFDVIGH
                     PDVIKVFGARLATGLEGPGEEGPLRGYYDALTAAAASSGTCLEVSSAGLRRPVEEAYP
                     AMTLLKMACERGVGITLASDAHEPEEVASRYDELVSWAREAGYTKLTIFDQRTGRQAA
                     MG"
BASE COUNT         5081 a         6317 c         7351 g         5904 t
ORIGIN      
        1 gggtttttat cctgccagaa caccgaccga ataaagatga tgggggtagt aatgtgccaa
       61 gcgaggccga tgtgaaaaac ctgactggct tgtcgggcga tgaagccgcg aggaggctgg
      121 cagaggaggg accgaacgag cttccgacta gcaagaagtg gtcaatatgg gcaatagtcc
      181 tggaagtagt gggagaaccg atgttcatgc tcttggtcgg gtgcggcgtt ctttatctct
      241 tgctcggcga cactaaagag gcggctatgc tcatgggctt cgtattcgtc gtaatgggga
      301 taacgatcta ccaggaacgg aagaccgaaa atgctttgga ggccctgaga gatctctcta
      361 gtccacgcgc tctggtcata agggacgggc gacaattgag gataccagga cgagaggtag
      421 tgtgcgggga catattccta gtctctgaag gagatcgcat tcctgcggac gctgttctta
      481 tatggggaat taatctgtcc gtaaatgaat cgttgctgac gggagagtct cttcctgtga
      541 ggaaggttac agctgaaggg cggcctgagt cgatcgacat gggacgtccg ggaggagaag
      601 aaaccccgca tctgtactcg ggaactctgg ttgtgcaagg ccaaggagtt gccttggtta
      661 aagccattgg cattcagact gagctggggc aaatagggaa ggcattacag acagtcgagc
      721 tagaggagac tgcccttagc cgcgaaacag ggcgaatggt gcgcaaactg gcgatactgg
      781 gcttcgtgct cttcgcaatc gtagtcttgg cttacggcgc cacgcgagga aactggattg
      841 atgggattct ggcaggaata acgcttgcaa tggctatgct gcctgaagaa tttccagtgg
      901 tcttgacgat attccttgcc ctcggggcat ggagaatgtc gcaaaaaaac gtgctgacac
      961 ggcgtatgcc cgctatagaa acattgggcg ccgccactgt gctctgtgtg gacaagaccg
     1021 gaacacttac gcagaaccgg atgtctgtaa gcagactgtc ggttgcagga gaactctacg
     1081 aggccgtagg ggcgcgggtg gaggttctgc ccgacaagtt tcacgagatc gtggaatttg
     1141 ccatcctagc gagcgagaaa gacccgtttg accccatgga gaaggcccta acggagttcg
     1201 gccatgagca tctcatcaat acagagcatc tgcacgatga ttgggcattg gtggaggaat
     1261 atgcgctctc ccgcgagttc ttggcattgt cgcatgtttg gatgtcttca catgccgacg
     1321 gagccttcta tgtagtggct gccaaggggg ctcctgaagc cattgcagac ctgtgtcacc
     1381 tcgatgaggc gaaaacctgt ggcttaatgg acgaggtcag ccgtatggcc gctgacggtc
     1441 ttcgagtgct tggcgtagcc aaggctgagt atggggtggg cgatctgcct gagcggcagc
     1501 atgattttga gttcgaactc cttggattag tggggcttgc agacccaata aggccggctg
     1561 ttcccgatgc aattggcgag tgttaccagg cgggtatcag agttgtaatg ataaccggtg
     1621 attatcctgg cactgcgcag cacattgcgc gggagattgg cctcagaccg tcggatgtag
     1681 tgatcacagg atctgagctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 gagctgaggg agatggtgaa gacgtgttgc atctttgcac gcatggtccc cgagcagaaa
     1861 ctgaggttag tggaagcgct caaggttaac ggtgaggtcg tggcaatgac gggagatggc
     1921 gtgaacgacg cgccagcgct caaggctgcc aacataggaa ttgcaatggg aggacgggga
     1981 actgatgttg cgcgcgaggc tgcctcgcta gtcctgctag acgacgattt ttcctccatt
     2041 gttagggctg tgaggcaggg ccgcagaatc tacgacaacc tcagaaaggc cacggcttac
     2101 attttcgcga tacatattcc catagccggg ctttcactgc tcccagcgtt gtttggttgg
     2161 ccgctcgtgc ttcttccggt tcacgtggca ttcttggagc tcatcatcga tccggcatgc
     2221 tccgtagtat tcgagatgga gcaggaagag gccgacgtga tgaggaggcc gccccgggat
     2281 cctgccaaac ccttgttcgg cgccgagctc atgaggatcg gaattgccca aggccttagc
     2341 gtcatggcgt tcgtgactgc ggtctacatc atctccctgt tcctcttgga tcgcggagag
     2401 atggaggcac ggagtctgac cttcacaacg cttgttctgg cgaatcttgg actgattctt
     2461 gccaaccgtt cgtgggaatc cacaacgatg gcagggtgga gaaggcctaa cccggccttg
     2521 aagtgggttg ttggtggagc tatcgtgttc cttgcgacag ttttaagcgt tcccgcgttg
     2581 aggaccttgt tccattttac cgtattgcat cctgtggata ttgctatctg ctttgcgaca
     2641 gggacattga gtattgtatg gttcgaggta ttcaaggcgc tgcgcatgga tgtagtcagt
     2701 tagtgggatg acccggccaa tgacgctgaa tttcagaaag ggaaatgcgg ggttctgctt
     2761 gaatgcgtaa ccccgcattt cctacgcttt ggtgcaatgg gtgtatgaga tgtttgctgg
     2821 ttatcagaat ttcattccaa gagatagcct gtggctatcc ggcaggatac tgtgggtctg
     2881 atatgcgtag tcgatggtga acatgctggc tgagaagcct gcgccggctg tgagggagtt
     2941 ttgcttgctt ccgaaagctc cccctgctct tagcaggatt cggccaagct ggaattcacc
     3001 gcctattctg gtttcaagct cgtccttata tatcagatca gctgccaaaa gaagtctgtc
     3061 aacgggatag aaggcgactc ctgcacctac ggatcggtcg aacgcatcgg cgtaacccga
     3121 cgagtatttc agctcgccac ctacgttttt cacgacggct ccgactctca gtttcttgct
     3181 tggagctata aacaggagac cgatgtcgcc ggtgactccc tttcccggat tgtcgggcaa
     3241 ggcttgggag tagtacttga gagccgctcc tatccctaga ttggggagag cttctattcc
     3301 accacagacg atgactgtaa agtcactcac gccgaacacg ccagtctcgt tggcgaactc
     3361 gtctgttccc tcgattcccg acgcgtcgag cctcaatcct cccacgccca catgcttttg
     3421 ggcatatgcc gcagccatga accccgcagc tccatactgc tgtgtgtata gagaaccgac
     3481 atgacgttcg ctcatgaagg ccaaacctgc ggggttatag taaaccgcgg cggaatcgtc
     3541 tgccacagct atgtgcgcgc cgcccattcc aagcgcccgt gcccccatcc ctatgtccat
     3601 catcgccgcc gttcctaccg ggtcatcggc ggcaagcgcc gacgcgctca tggtcaggac
     3661 gagaatgagt gtaaacgcca gcgctgatat tctcactcgc attagccgct cctccttagg
     3721 tgagtgtagt gttcgggagt tgctgggcca ccgggcggcc gcgtgggcga ccgcccgcgc
     3781 ccatactgtt caagccgggt ttagcggctg attaccagtc ttccaacttc ggttttctct
     3841 tgcccgctta ctgcgatgta gagatagact ccgttggcaa tcggtctgtc gcctgacctg
     3901 aggttccact catgagcatg tgccgcggcc ggaagctctg cgctgtaaac aaggcgtcct
     3961 gcgatgtcgt acacgtagag cgttgcgtcg gcggcaagat catagtagaa ggtcacgcca
     4021 tccttcgccg ggttcggagc tgccacgatc gcgtgtgtta gtacaacgat ggtgaactcg
     4081 tcgctcatgg cctgtccggt agcatcgctt ggatcggtgg ccgtgacttt tatcctgtag
     4141 cgtccgccct gcttgacact ggaggtatcc caaacgcatg taccggtatt tgcctgatta
     4201 gcggcgatgg tctgccaatc ctgacctccc gcgggtttgt actcgagggt gatcttgagc
     4261 gtgttcgcgt cgtcgtcagg gtcgacagcc tcccactcga tggtttgcat gcccgtccag
     4321 actgtgccga cctccggttt gactattgtt acctctggcg ggagattcac gactgttatc
     4381 tccccttcag cggtgcccca aagaccgccg ttgtcagtta cagtgagtgt cactgtgtac
     4441 ttgcctttgg aggcatacct gtgactcggg ttctgctggt cgctttccgc gccgtcgccg
     4501 aacgtccaat gccaggcggc tatagggccg tcgtccgtgg actcgtcaat gaattccaca
     4561 ttgtcatgaa ccgttggcga agcaggcgtg aagctgaacg cagccactgg tgccgcgttg
     4621 actacctcca cgtcatgttc gacgctgctt gcagtatagt cgtcatctat caccatgagc
     4681 ttcacagtga agatgcccga tgttgcgtat gtgtgagtgg gctcgggtgc gacgctcgtg
     4741 gtgtcgtctc cgaactccca gaagtacgcg attatcccgg catcatctgt ggattcgttg
     4801 gtgaatgtca cgacttcgcc tgccttggga gactccggtg agaagctgaa cgctgcgatc
     4861 ggcgctgcgt tccccaccac gatactcttc tccacggttc ctttcagctc gccgttgtcg
     4921 gtgacggtga gtgcgattgt gaactcgccc ttctcggcgt accgatgctc cgggttctgt
     4981 tcagagctgt ccgtgccgtc tccgaactcc cagtgccatg ccactatccg accgtcgtct
     5041 gtcgatttgt cggtaaactt cacaacgtcg cgccttgtag cagacgacgg agtgaagccg
     5101 aagtcggcgg ttggggcatt gttgatgatg gtaaactcac ccgtgacgaa ctcgtggtac
     5161 gcgcctgtgg ggtcagtgac tcttgctcgc aaccggaagc tgtcgccagc tttgcctact
     5221 tcaatggtgt tccattcgaa agcgcctgtg ttggaaagtc cggttccgag gtcgtgccac
     5281 tcgacgccgt cgcgggtcca ggcaaggtcg atcaggaggt tgtctccatc atcatcaggg
     5341 tctgttgccg tccattcgac ctcacatgtc ccgtgcagtt cggctccgcc gacaggcgcc
     5401 gtgatgacga cgctaggtgc ggcgtttagc ttgagtttta cggggatggt cgtaaatggg
     5461 cggtatgggt cgttggccga cacatacagg ttgagcgaat atgtgccgta cggagcgtcg
     5521 ggagatccga gtgtgagaac tacgtcctga tatccccctg gcacaaggta tcctgatgtc
     5581 gggttgactt tcaggatcga tccgacaggc gcaaaaccta ttgcaagatc gttttcgatg
     5641 taagcggcgt tgtagagtac ctgtaggccg tcggttccgg tggtgttctc gatcccaact
     5701 gtggctgaat tgagaagccc aaccatgtta agatactggt agattattgt tccgttgggc
     5761 ttcaggatta cctggaacgt gtagcttgcc gacccgccca atctgggcac gttcttgtac
     5821 tcaacgataa agcgctcgcc cgcggtgtcg ttgtagtagt agatttctcc gccaccgcct
     5881 ggatagaggt catcccagaa tacggcgagc atatcattgg gctttgctga cgtgggaatc
     5941 ggcgcatttg tccagtagta gttaatggaa tcgggatcga agctgagata accgttcgcg
     6001 cttatgccga cttgcgtctt catctggccg tagaacggga aggtgaaggg caggggcacc
     6061 attaccccgt cgtcatcgcc caaatacacg cgtgttccga gtccgctgat ttctacccaa
     6121 tcgaaagcag gcccaccggg gttcttgctg tcgatccaga cgtagccgta atgatcaggt
     6181 cctccagcgc cggcgcccgc gtaacccctt ggctcggaag cgtagtcgcc ctttatcggc
     6241 tctgaagcgg cgtacgcttt ccattcgggg ctttcggcga agctgggagc gactgcaatt
     6301 tcaaagttca tcggcgcaat gccggtgttg ctcactgtta agatggtttg cttttcctct
     6361 ccgacttgga tgaccgcatc gaactcttca ggttctacgc ccagcacagg cagccacttg
     6421 actgtcactg tcacctcggc ggttgcctcg acgtcattct catcaactac gtggaagatc
     6481 gctttgtata cgccgaggtc ggtgtagacg tgctcggttg tagcttcatg cactaggtcg
     6541 cttccatcgc cgaactccca ccacatgtca gttatcggct tggcgcctgc ttcaacagtg
     6601 gcggcgaagg cgactgtaag cggcggttgg ccgatctcag gagtagcggt gacttcgact
     6661 atggtgggtc ctggttcaat gtggacgatg aggtccacaa agagcgctgg gttgaccgga
     6721 tcgtttgtca ggatctcgag atacttgcgc acatcctgac cgggcttgcc gagctcggcg
     6781 gctttgatcg tcacagcaag gtctgcggtc tgccctgtag gcacagtccc gctcgtaggc
     6841 tggacaacta tgccggcgct caacggcatc tcggtatcga tcaggtagac gtagttgtcc
     6901 gtctgtgatg cgacccatag gttgccgtca tcgttgagcg ccaggcccgc gccggagtag
     6961 tccccacggt acgggtgctg gaactgatgg attacggcga gagattccat gtctactccg
     7021 tagatcatgt caggagcgga gttggtggat acccatagca cgccgtcggg gtgatactcc
     7081 agccctgcta tgctcacagg gaaggagtat gccgcgatta ctgcgccggg agtagaccag
     7141 ctcggtccca tgatgtggta aatgatatcc tcgttccatc cgccaatgta gaaggtgtca
     7201 gtgtcggcgt cgtaaccgag gccgcgctgt gaaacagagg tccatgcgcc gccagaggtt
     7261 atcttggtca caacctctcc tgttgcgggg ttgaggccgt agattccgtt gtcgccaccc
     7321 acgtttacct gccacatcag gtttcgattg gagtcatatg ccatgtcgcc gggccatgat
     7381 cctgcccacg gcgtgttgaa gatggtgcct gtgaaaacgc cttccggtgt gatgatgtag
     7441 tcgtttttgg gcaggggatc ggcgagccac acgtttgtgg cgtcgaagcc gaggccccag
     7501 gcgatttcaa ctgcagctgg cgcgggccat gacttgacta cgtcgcctac gccgccgggt
     7561 tggatgttgg cagcgattct ctcaactgcg gctgggtcga atggcatgta gaggccttct
     7621 gccgtggccg cgttgggatc gatcactgtg cctatccgtt caggcatggc cacgtgaggg
     7681 gcgctgccgc ccttcacctt cacgctgaaa gtgaggggag cgttgccatc cacgttgccg
     7741 actgtgacgg tctcggtcgc ttcgccattg ggaggcagcg tgatctcgat caatgcggga
     7801 tccactgttg catgtggcga gtgtttcacg actatgtcga tgcttgcctc cgcagttgcg
     7861 ccgagctggt cgattactgt gaatgtggct gtatagttgc cgggcaacgt gtaagtatgg
     7921 actacgtcca gctctgtgga ggatatcccg tcgccgaagt tccaaccgta acttgcgacc
     7981 ggcacctcgg gcgcgacgaa tgctgcgtgg aatgtaacct ccagaggagg ctcgccgaat
     8041 gtgggttcag cagttgcttt cgtgattgca ggaggttcgg ctacctgtag tgtcactggc
     8101 acaacgatta gcgggttttc gacgtcgttg gttgtgagca ccacattgcc ctcatgcggt
     8161 cctgggctga gtttttcgct gttggctgtg atcgtgatct gcactgatcc gcccgcaggt
     8221 acgctgcctt ctgcaggctg ccaactcagc cagtcgccgg agccgatcgg tccgaggccg
     8281 gtttccacta ggtacatctt attctcgccc cagcttgcga cccacaggtt gccgtcgcga
     8341 gcaagttcgc acccgactcc catgtaggct ccgcccgctg gatgctggaa ctgggcaatg
     8401 gttgcgtgcg ttgttgcgtt gacgaagtag atcatgtcag gcgaagagtt gcatgtgact
     8461 atcagaacgt cagcaacagg gtggtaagca agccctgcta tgctgacagg catggaccat
     8521 tgttctatta cagcgccggg gctgtcccag ctttccccct ttaacttgta gattacgtct
     8581 tcgttccatc cgccgatata gaaggtatcg tcgttggcgt tgtatgccag gcctcgctgg
     8641 gatacactgg cccacgctgt attgcgtatg gatccgacgg tctggccact tgcagggtcg
     8701 accttgtaga tggcattgtc accgccgacg tttacctgcc agatgtaggt tccatcgaag
     8761 gccatgtcgc cggaccagga gccgccgaaa tcagctgacc accacctgcc ggtgtatgcg
     8821 ccatccgatg taaccacata gtcatctgtt gggtcgactg cgccgtctgc gatcactacc
     8881 tcgtcggagt ccaagagaac tccgacgccc cacggatcgc ctatgggcga cggacactgc
     8941 cagctcttta tcaccgaacc tacagcttcg ggcagccact ttgacctctc ggggttctga
     9001 ttgggtagat acagcccgaa agctgtcttg gcctcagtat ccttgatatc ctcaggcgcg
     9061 atgagtcttt ccggtgtttg gatcatgctt gggaactcgc cgctcccgaa gaggagagca
     9121 cctggccctg cgttcgagag agtgagtatt cctgttgccg tttggcctcc gccaactgtg
     9181 aagctgaact gttttggggt ccagcttgca gatgcggatt gcgcgacagt caccgtcact
     9241 gtggcagtgg cggtatatcc atcattgtcc acagctgtga acacggctgt atacacgcct
     9301 gcggcggcgt agatgtggtt ggcaacgaac tcatggacaa ctggcgatcc gtcgccaaac
     9361 gaccaatact tgtcaattat cgaaccgtcg gggtcacatg ctgcccccac aaactggaac
     9421 gatgtttccg gaggtccctg ctggggattg gcagcacacg ccgtgattgt aggcgggctg
     9481 ttcgggatca catggatatg gacggggact atcgttcttg gatttcgaat atcgttggtg
     9541 tctagcacaa ctgcgccgtc caacgtccca gatccgacaa gtgccaggtc gaacgtgatg
     9601 tccaaaccga tgctttctcc gggcgccagt tcgccttcgg tgggctcaac cgacatccag
     9661 ctgatgggtt tgattctgat agccaggttg ttgtgcatgt agcctggtgt gttgtagagc
     9721 acttgcaggc cgtctgttcc tgtggcattt tcgatgccga tcgtgccgtc cttttcatag
     9781 gtagcgctgg cgaattgcat gttgagatac tggtagacta ttgttccgtt gggatagagt
     9841 atcgcctgga acgtgtattg gccgttgttg tagtaccgag gcactttgtt ccactggact
     9901 atgaaacggt tgttgactgc gtcatagtag tggaaaacgc cgccgtcagc gggcgcctgg
     9961 ggcgggttga ggtcgtccca gtagacggcc atcatgtcgt ttggctcagc tggattggga
    10021 attgctacgt tggtgtaagc agtccccttt gtgccaaatg tcagataacc gtcggaacag
    10081 atacggatct ccgtcttgat gtctccgtag aacgggaagt cgaatgggag gttgactact
    10141 ctggagtcat cgccggagac tggcagctta gtgcctatcc cgctgatttc gacccactcg
    10201 aatatgggtc cgccgggttc gtctgaatcg cgccacatgt agccgaatcc gtcgggaccg
    10261 ccggcgccga acgggtcgag ttcgccggct tcagcaggaa tccccgcggt ataggctgta
    10321 gccgcaaact ccagtgtcgc cactcccgtg ttggtcacgg tcagcgtttc tgtacgctgt
    10381 ctgtgggcgc ggatcacttg gttgaatgaa gcgggatcta cggccacctg gggcagatcg
    10441 gttacgacta tgtctatttc tgccgcggcc tcgaacccgt cgtcatccac aactatcact
    10501 gtagcggtat agttgccttc tgccatgtag gtatggatag ggttgaacgt cccgctcaca
    10561 accgggctgc cgtcgccgaa gtcccatata gcgtcgacga cctcgccgtc ctgatcatat
    10621 gcatccactg aaaagtgggc ttcaagcggg gcgctaccgg cccacgggtt ggccgacgcc
    10681 gaccttatag tcgggtttat gcgcgacctg acgaacatgt aggtatccac atcgactttg
    10741 gggttcgccg ggtcgttgct ttcgatctct actacagcct tccaggtgcc cttgggcaaa
    10801 tggtcagcga agaacatcag gtcgaacgtg tccacttctc cgggctcgac agttccccct
    10861 gaactgcttg agacgatcca acccgggagg aacatgatac ccatgtcgtc gtggacgtag
    10921 ttcttgttgt atgctacttc caatcctact gtgcccgtgg cgttctcgat gccgattgtg
    10981 gcttcgttca ggcgtgttcc ggccatagtc aggtactggt acatgatcac gccttggggc
    11041 gaaagccgta cctggaatgt gtaaggtccg ccgctgctca gcctgggcac attagtatac
    11101 tgaactatga agatgtcttc atcaccgtag tagtggcaag taccgctaga gctcagggtg
    11161 aggtcatccc agaataccgc gagcagatcg ttgggatcgg cagtattcgg gatagtggtg
    11221 ttggtccatg cgttcagttt gccgccgaag gtaagatacc cgttggaaca tatggcgatt
    11281 tgcgatttct tctgcccgta gaacgggaac tcgaatggga ggtcaactac gacgccattg
    11341 tcgtcttcca atgtaatctg ggttcctatg ggccttatat cttcccattc gggaggctcc
    11401 atgccgggct cgtcggtgtc gagccagaag tagccgaagg aatcaggtcc tcctgcgcct
    11461 agcctggctt cgcccgatgc attcatcttc ccgaccagcc tgatgttgac gctgtaattg
    11521 agttcgccaa ggcctgtgtt gctcacggtg acctggcgag ttgtttcttc tgcccaccac
    11581 aggtcgacgt ggatttcatc tggatccact cctattacgg gcgggaagaa gaccttgatg
    11641 gtcagggaag cggtggcttc gagaccctcg ccgtcgaccg cgtggaatgt ggctgtgaaa
    11701 tcgccttgct cagtgtatgt gtgcacagca ttgaattcgt gcactggtgt gctaccgtcg
    11761 ccgaagtccc accacttgtc cacgatctcg ccaccaggcg aaatcgcaga cgcggtaaac
    11821 gtgacctcca gcggcggcgg accgaacctg ggctgggcat cgatcgaggt tatcaccggc
    11881 ccgcccaatt cgaggagcgc cgcggctatg ttcaatctgc cgttgcttac cacgttgccc
    11941 tcgtacgcgg gtttcacgtc gacagtgtta aggagtatgt ccttgactgt ctgttgcccg
    12001 ggtattgatc ccggcgcact ggggtacagg ggcaggcctg gcctgaggga gtgcaacagg
    12061 gctgccgcgc ctgacacgtg aggtgttgcc atcgacgttc cgtagaagaa gtcatatgcc
    12121 tctccaggct gcgatggagg aggatccggc ggaatagttg agaggatata tccgccaggc
    12181 gcgtacaggt cgacgctggt cggaccccag cacgtgctcc accagcccgg gtagttgcat
    12241 ggggtatcgt tctgcatcat cgcagccacg gatataatgt tggcactgtc gtaactcgat
    12301 ggatagtgcg ggtttatgtc tgtgttctgt ccgctgtttc cggcagcgca cgacactata
    12361 gcatgtgtcg cctctatggc gtctttgagc gcctgattgt agcctccccc gccccagctg
    12421 cagcttatca ccttcgcgcc cttgttggcc gcgtagttga aagcaaggat ggcatcggag
    12481 gtatagcctc cttccgggcc gaggaatttt aggatcatga tcttgatatt ccagttgatg
    12541 ccggcgacgc caatcgcgtt gtttgtaatg gcgccgatgg tgccggatgt gtgagttccg
    12601 tgctcgtcat tgagatatcc gtattggtcg cgttcgcctg gatcccatac ggagttgtcg
    12661 ccattgaacc agtcccatcc ccagatgtcg tcgatgtaac cgtttccgtc atcatcgatg
    12721 ccgttgccgg ggatctcgcc tgggttgacc cagatgttcg gtgcgagatc tggatgtagt
    12781 atgtaggatc cggtgtctat gatagctacg actgtttctt cggacccggt gcatatgccc
    12841 catgcttctg gggcgtctat gtcggcgtca tctacaggat cgcccgacat gcgcggatcc
    12901 ttgaactggc agttctggtt gtgcagaccc cacatcttgt tgaattgcgg atcgttgggg
    12961 aagattgggg catccccata tgcctggtcc ttgtagcgga tgtagtctgg ttcggcgtac
    13021 agtatgcccg gggtcttgct cagtctgtcc actgctgtgt cggggcttac gccgtctgga
    13081 agctccacta tgccgattct cagtccgtgc gggaaattgg cgctgacgcc gaaatccgca
    13141 acacggtcta cagaatagcc gagggcagca atggattggg ttgcatgttc cgaggataca
    13201 acatcagaga acctcacgat taccctgctg ggatggtaga tgtcgggacc cggttgccac
    13261 ccgccccgcg gcaagcctga gggcgtgctc tgcgccgctc ctactgcggt tgcaagggct
    13321 agagccaacg acatgatcag tagggcaatc catggagtct ttcgccagtg gctcttggcc
    13381 atatgtcgat tactcccctt tcatgctgat gatttcattg tgtttgggaa cgcaaacctg
    13441 aagtggacgt gaaaacgtta atactgcttg tcccgctcgt cacccccttg gtccgggcag
    13501 gctaaaactg cctttctggg tttggcaatc agctgccata cgaattctgt gcggtgcccg
    13561 caaatcccta aatactacat attgcggccc caatcctgcc aactatggat aattatgata
    13621 ataaacgaat taggacgaat tcctctgcgg tttgcatgag ctgatcggaa tggggcgtac
    13681 ttgtggaaac gctaggagga ttccattaag aaaagttgaa caagtaatgg gctcgtccga
    13741 tagctggctc gctcctgttt aaacagaacg gtggttatcg agagggtggg tggtgggggc
    13801 tggctgaagc gacggaccgg ttgccgaaaa tgcttaatag ggagcgtcag ggatgtcagt
    13861 ggttagtgac aatggtgata caagggttgt ttcagggaat gcaggcacag atagtggcat
    13921 gtcgaagcaa gggacatctg cggatggcaa aatgcgtatt gaaacggatt cgctggggaa
    13981 aaggcaggtg ccggtcgacg cgtactacgg gatacagact cttcgcgggc aagaaaactt
    14041 tcagatcaca gggctttcgg ttcacccggc acttattcgt gggttgatca tggttaagaa
    14101 ggcgacggct cttgcccaca gcaagttagg ccagatcccg agccgaatcg ggcaggctat
    14161 agctgaggcc gccgacgacg ctttggctgg gaagctcgac agcagtttca tgcttgaccc
    14221 aatccaaggc ggagctggaa catcgatcaa catgaatgta aacgaggttc ttgcaaacag
    14281 agccatagag atcctcggcg gggtaaaggg cgattactct ttagtgagcc ctttgaacca
    14341 tgtcaatatg gggcagtcta cgaatgatgc ggtgcccacg ggggcgcgga ttgctctgct
    14401 tacacagctt gaagggacaa ttgaaggact ggacgctctt gcgtgtgcgc tcgaggcgaa
    14461 gtccgaagag ttcaaagacg ttgtaaagat ggggaggacc cacttgcagg atgcggtgcc
    14521 tatcaccgca gggcaggaat ttggggcttg ggcgaaggca ctgaggcgcg acattgcgcg
    14581 gatcgaagcg gccagtgaag gtcttcacat catcaacctg ggcggaacgg cggtaggcac
    14641 tgggctcaat gtggatataa ggttcattca tcttgtctgc gaggaactct cgaagataag
    14701 cggttatcct ctccaaactg cggaagactt ggtggattgc actcagaacg tggacgtatt
    14761 cgccaatctt tcgtcgtgca tgaaggtgtg cgcggtcaat ctctgcaaga tagcgaatga
    14821 tatgaggctc ttggcctccg gacctatagc cggccttcat gaactgttgc ttcctcctgt
    14881 tcaaccgggt tcatcaataa tgccggcgaa agttaaccct gtgatacctg aggttgtgaa
    14941 ccaagtcgca tttcagatca taggaaatga tctatcggtt actatggctg cccaggcagg
    15001 ccagcttgaa ctcaatgtat tcatgcctgt gctgctcttc aatctcttgc aatctgcgga
    15061 cattctgggc aacgcggcga gaaccttcgc ggataagtgc gtggttggca tcgaggtgaa
    15121 cagggaacgc tgccttaaca tggttgagac caatgtcgga gtagtgactg cattggttcc
    15181 gtacctgact taccaggtag cttgcgacgt ggccaaggag gcgcaggcca ctgggaagac
    15241 ggtaagaagc attgtgctgg aacggggctt gctttcggaa gaacagctcg accacgtgct
    15301 gtcgtttagc catttgacca gccccagcgg tatggatggg gcgtagcctg tacacaggct
    15361 gaaagcctaa agacatctta agagggctcc gaacggcagt ctgttgtcct ggcaggttag
    15421 cgattcgtcg ctgcgcttac ggcaacgcct ctgctaggca gattgcactt cggagccctt
    15481 ttttttatcc tgttatctcg tggaccttta tcaggtttgt ggttcctgac atgcccaccg
    15541 ggacgcctgc cgtgatgatc acagtgtcgc cctcgcgaac gaatccggct tcctgtgcct
    15601 tggcgacagc ggtcgagatc atctcgtctg tcgttgcaat atcgtgacct tgcacggggt
    15661 atactcccca gacaaggttg agaatccttg cagtctgttc gtcggttgcg acagccacga
    15721 tcggggtctt cggcctgtgc tttgctatca tccgggcggt ccagcccgat tttgttgaag
    15781 tgatgatggc tgcagctttc agttccgttg caatctgcac cgtggcatgg ccgatcgcgt
    15841 cagttgcggt tattgctgcg cttccgccac gctgaaccgg agcttcggcg aagtgcccgg
    15901 ctgtttcggt gcgttcaacg actctggcca tggtacgtac ggactcgaca ggatattttc
    15961 cgctggcagt ctcgccgctg agcattacgg cgtcagtccc gtcgaggact gcattggcca
    16021 catcgctcac ttcggcgcgg gtgggatgag ggtttctgat catcgagtcc agcatctgag
    16081 tggcggtaat caccggcttg cctgccctgc ggcatttctg tatcagttgc ttctggatga
    16141 tcggaacatc ttcggcggga agttcgacgc cgaggtcgcc acgggcgacc atcaacccat
    16201 ctgcagacct gaggatagag ttgatgttct cgatcccttc tctgctctcg atctttgcaa
    16261 tgaccggaag cgatacgccg cgtgactcgg tgaaagactt tatttcctgc acggaagagg
    16321 ccgttcggat aaaggaggcg gcaataatgt cgatcccgtg ttctatgccg aaggccagat
    16381 ccatcttgtc ctctgatgac agtgtcggaa ggtctatcct tcgccctggg acagacaacc
    16441 gctttcggtc gctgagtact cccccgacga tgacttcaca ttcgactgca ggacctttta
    16501 cggaacgaac ttccatctcc agctgaccgt catctaacag aatcttgtcg ccctgcgaaa
    16561 ccaaaatggg caggccgggg tagttcaccg atacgatctc ggaattcccc agaagcggct
    16621 gtgttgtgat gatgaatgtg tcgcccggag cgagcgtgat tgaacctgat tcgaatgctc
    16681 cgattctcaa ctccggtccc ttggtgtcga gcatgatcgg gatgttcttc ccgcattctg
    16741 ccgccaccgc gcgtatgcgg cggattctct cggcatgttc ctcgtggacg ccatgggaga
    16801 agttgagccg cgcaacatcc atgccggcgt ttgccaaact tcgcaacgtg tccagatcgt
    16861 cgctggccgg ccctattgta catacgattt tagttttccg cattactgac acctcttgag
    16921 tatggacctt gcccggttgc agtctgccca aaaaatagat tcgccagtgt tcaccttttc
    16981 gaccctgtgc agttggctac agaaaggagt agccgcttgc cagaggctgc tagtcgcagc
    17041 tggatccaga gggtacaggt cgcccgcaaa agatgcagta acgcgcaccg gatactatcg
    17101 atgcaccgca cgcgcacttt ggagcgtctg agcatgattc ttcagcctct tccgcttctt
    17161 cctcagaaac atcagcaggc tctggttcgg cggccgattc ttcgatttcg gttgcctcag
    17221 gtttttcttc accatcggac tcttctctaa tgtttgccag agcctcttct gcttcagcga
    17281 tttccttgtc tatttcggtt atctctgccg ctttggcata gagtctgtcg aagttgaagg
    17341 cgtttttacg aagcatctcg tatgcctcct cgcccaattc ttccatgacc ttatgcctgt
    17401 ccgccttgag gtcgccgatt ttcgatttga ttcgcgtgga gtcccatagt tccttggttt
    17461 tgcttcctac tatgtcaaca cctgtaccta ttccttttac caccttgtcg attatgtcgg
    17521 tcacgaagac tcgcctcctg tcgttatcat gtgagttata aattcgcctc atttcacata
    17581 tatcctccgt ccgtgttagt catggcgggt tgcgcccttt ggcaggcagc gcatcccata
    17641 tcatgtgcat cacagaatac gtcgaccatg cgagttgcat gactgcaaaa gtgttggact
    17701 gcaaaccttg cgatttggag ggaaacaccg agcgtttcaa gaataaataa gctgtacggc
    17761 gcgggcctgt agaataggga tttccagaga cgtgcgccgg gatcatcgta agatgggagc
    17821 gggcgagcag gcatgataac tttgaaggaa ttcttggccc gagaggttgt tccggccctc
    17881 ggttgtacag agcccggcgc agttgcgctg gctgcagctc gggctagaca agaattgggt
    17941 ggcgagtata tagagcgcat aagcgccgat gtcagtgaca gcatatacaa gaacgggatg
    18001 aacgtcggag tgccgggggc gagcggcgaa acagggaatt cgctcgcggc tgctatggga
    18061 gcgcttgcag gcagggccga tttgggattg gaagtgctgc gcgactgcac ccctgaagat
    18121 gttgcagctg caaaacgcat gatcagcgaa ggccgggttg aactcaattg catgccccac
    18181 gtgtgcggtg tttatgtaga ggtgacggtg gaatcgcgga ctcatgctgc tacctgcgtt
    18241 atagaagggg agcattccaa catagtcagg gtggacgtag acggcgaaac tgtcttcagc
    18301 aaagaggaag ccgccccagc ggggtcgacg ttccctcgcg atgacggtaa tgtccttgct
    18361 gaccatgcca atgagcaaga gactgacggc tttcttgagg ggatcgatta tcggactttg
    18421 ctcagccttg ccgatgatat ggggtctgac gaggtagaat atgtgatgcg tggcgtggaa
    18481 atgaatatgg ccatcgccga atatggccta acttccgaat ccaaatcggg gctgggagcg
    18541 ggcaaagcca tatggaagct catgagcgaa aacaagatcg aaaaggattt ggccttcctc
    18601 atccgttcct actcgagcgc agcctcagat gcgagaatgg ccggggttca attgccgacg
    18661 atgagtgcgg caggaagcgg caaccaggga ataacggcga taatcccggt ggcgctggcc
    18721 ggtgaatcca tgggcaaatc gagggaggag atagctcgag cagtcacagt gagcttgctt
    18781 tccacaagtt tcgtgcggag taggacgggg aggctcacgc cagtgtgcgg ctgttctgtg
    18841 gctgcgggcg ctggggcggc ggccgggatt gcgtgccttt tgggaggcaa ctggatgcac
    18901 tccgcccgcg ccatgcagtt agtgttggct aatactgctg gcatagtgtg cgatggggct
    18961 aaggggacgt gtgccctgag ggtcggggcg gctgcatgtg aggcatactt ggctgcgctc
    19021 atagcggtga acgacactgg catcgatgac gcacagggta tcgtggacat caccatcgaa
    19081 gggacagcgc agaatgttgc acggctgaat gctgaaggaa tgaaagatgt ggatcgtgtc
    19141 ctgatcgaga tattggaaga acgagtccga agaggagatg cgttgtagcc atgagacgcg
    19201 tttatgccgg gactcccatg gtggggggca tatccgtagg aacggtgaaa gcactggcgc
    19261 ccaagcgtct tcctgaagcg ggggctggcg tcgttcgccc ggttggagcc ggcagggaag
    19321 gggccgattg gacagcggca ttatccctcc caatgtggga gtatgctcag atatgggctg
    19381 caacagtggc cactgcttcg cttctcacaa cagccctgca acggaaggcc gaggatgaac
    19441 ttgggggggc tgctgcggat atctttgaag cgcagcacgc tattgtctcc gaccctatgt
    19501 tggaggcctc tgttttcagc agggttgagg agggcatccc gcttccgcag gcagtttccg
    19561 gagctattac cgagattgtg gccggtctgg aggcgtcgga tgacgaggca actcgtgcaa
    19621 gggctgaaga tgtttatgac gttggcctgc gctttatcac cactttagcg ggtattggag
    19681 cagcgtctgc catggacgac ggccaattcg agcctgtgct cgtggttggt ggctctccta
    19741 gtgaagaggg ggtccttgtc gctcatgatc tgctcccgtc tgagctggtg atgctgaacc
    19801 gcaagatgat gttgggtctc gttcttgagg ctggatccgt gcactcaagt attgacgtga
    19861 ttgcggaagc gcttgggatt ccagtgatta tccaagctgc gggggtgctg tcggggttag
    19921 ttgacgggga gagcattatc gttgatgcgg ataatgggac cgtcattctc gagccctatg
    19981 aggatgagtt gaaggagtat gcctctagga tactcaaagc cggccaccag actttttatc
    20041 aagcaacaca ggcgataatg cacgacgtgc gcaagcctcc ggaatccttg agttaggcgt
    20101 tgggacatca gcatatcttc ccagatgtct ttgagttccg acatgtcaga atgatgttgt
    20161 taatctggaa acgagatgcg ttttcatctt tagactccaa aaaggacatc cctagatggc
    20221 gtggtggata atgcatgata gacaaggtga cgcgcggcaa gatcttcgac attagacggt
    20281 attctgttca cgatggccct ggaataagga ccgccgtctt cttcaagggt tgtcctgcca
    20341 ggtgcctttg gtgtcacaat cccgaaagcc aaaggcgtga gcaagaagtg atgtattggc
    20401 ccaacaaatg caggggctgt ggactttgcg cttctgtgtg tgatgtagga gccgtgcgcg
    20461 tggaacatgg gcgcccggtg actgcgtggg agttgtgcac tggatgcggg aagtgtgcag
    20521 aagtttgccc ggctcgagcc aggacaatcg tgggccgtga agtttccgtt tccgaagtga
    20581 tggacgagat cgaaatggac agagccttct atgatgagtc tggcggcgga atcacattca
    20641 ccggcggcga gcctctggcc caaccggcat tcctatgcga actggcgcag acatgcaaga
    20701 aagtagaact ccacaccgca gtcgatacta gcggcattgc agcccgccac gtagtggagc
    20761 tagtggcgcc ctttgtagac ttgtggcttt atgacttgaa gatagtcgat cgcttcgcgc
    20821 acctctttgc tacgggatgt tcgaacgagc tcgccatctc caacctgcgt tggttggtga
    20881 aagagggata tgatgttacg ttgcgtattc cccttatccc cgggtacaat gatgataatg
    20941 agagccttgg cgactttatt gaattcatag ggtccctcga atggaaggac cctccccaag
    21001 cggccatcct gccttaccat cgtcttggga atgagaagta tgagaggctt ggacgtgtat
    21061 gctgcgttga agatttggat gagccggata gtcagaatat agatcgcgtc cgcagagttt
    21121 ttgaagctgt tggaatcgat gttcagatcg gaggctgaat ctgatgaata cgcgagtcag
    21181 tagattgcga cagcagagcc ttgacgctgt tccatatatc tccgccgaga gggctgaact
    21241 gatcacggaa ttctatgcca atgacaacac tccttcgcca gcactcaaaa gggcgcgtgc
    21301 ctttaggtat ttaatggagc acaaggccat ctgcatcaac gatggggaac tcatcgtcgg
    21361 cgaaaggggt cccgctccaa aagcgactcc gacctatcct gaactctgtt gccattccct
    21421 tgaagaccta gatattctca gtagccggga gaagataccg ttcgccgtct cgcctgagac
    21481 aatcagcatc tatgaggaga agataatccc ctattggagg gggaggtcca tgcgggatct
    21541 gatcttcacc gagatgactg atgaatggaa agctgcgtac gatgccggca ttttcactga
    21601 gttcatggaa cagcgagctc ccgggcatac agtggcggac gacaagatct atcgcaaggg
    21661 gtttaggcaa ctcagggagg atatcgcgcg gcacctggca tcccttgatt atctgggcga
    21721 tcccgaagct catgacaagg cagagcagct cagggcgatg gacatcgctg ccgaagccat
    21781 ctgcatattc gcgcgccgcc acgctcgatt ggccgaatcc atggcacggg aggagactga
    21841 tcctgtaagg gcctcagagt tgagacgcat agccgaaatt tgttcgtggg tgcctgaaaa
    21901 cccgcctcgg gacttttggg aagcattgca ggcctattgg ttcgtgcacc tcggagtgat
    21961 taccgagctg aatacgtggg attcgtttaa ccccggaagg ctggaccagc atctttaccc
    22021 gttttacaaa aaggggcttg aggacggcac gttaaccgaa gattctgccc gcgagctctt
    22081 gcagtgcttt tggatcaaat tcagcaatca gcccgctcct cctaaggttg gtgtgactgc
    22141 cgccgagagc ggtacgtata cggatttcgc gaacatcaac tgtggaggac tgactcctga
    22201 cgggcgagat ggggtcaatg gcttgacata cctcatacta gacgtgatag acgaaatgag
    22261 gctccttcag ccgagctcca acatacagct gtcggcgaag aacccggacc gctttcttaa
    22321 gcgtgccctt agaatagtga ggaaggggtg gggacagcct tcgatattca acgccgacat
    22381 ggtagtacag gagatgctca gggtgggaaa gagcttggag gacgccagat gcggcgggac
    22441 gtcggggtgt gtggagactg gcgctttcgg aaaggaggcc tacatcctca ctggctattt
    22501 caaccttcct aagattctgg aattgacact gtatgatggg gtggacatgc gcaccggaag
    22561 gcagcttggg cccaagacag ggcctgctgc gctgttcgaa acgtatgagg agctatggga
    22621 ggcatataca gaacagctca actacttcat agatgtaaag attaaaggga acaacgtgat
    22681 tgagaggctt tatgcaaagt acatgccggc gcccttcatg tcactgctga ttgatgactg
    22741 catcgcctta ggtcgagatt acaacgacgg aggagccaga tacaatacat cctacattca
    22801 gggagtagga attggcacta taacggattc cctttctgcc atcaggcatc acgtgtttga
    22861 tgagccccga gtcacaatgg atcaattgct ccaggcctta gaacgcgatt acgaggacag
    22921 ggaggatctg cgtcggctgc tctgggacga tactcctagg tatggaaatg atgatgacag
    22981 ggctgacacg ataatgactg acgtcttcaa aacgttcttt gatgcagttg acgggcggcc
    23041 gaatgtcaag ggtgggcggt attgcatcaa catgcttccc accacgtgcc atgtgtattt
    23101 cggttcggtt acaggggcta cccctgatgg ccgaagggcg tggcagcccc tgtcagaagg
    23161 gatctctccc gttcagggca gcgataggcg cgggcctact gcggtcttca aatccgcctc
    23221 caagatggat catgcgctga ccggcggcac tcttctcaac atgaaattct ctccgaagct
    23281 gctcgaagat gaagcaggca taaacaacct cgcgagcctg gtgcgtacct acttcaagct
    23341 tggaggacac catgtccagt tcaatgtggt aaaggcagaa acgttgcggg aggcgcaagc
    23401 ggatccagaa gcccatcgcg acttaatagt tcgcgtggca gggtatagcg attatttctg
    23461 cgacctttcc cgcgcattgc aggatgagat aatttccagg acggaacatc aagtgatgta
    23521 ggcaattcga taggggtgtg cgggatgctt gttgattatc acgtgcatct ggaaagggca
    23581 ggattgtcgc gcgccaacgc aatgcggttc gtggaccggg cgttggccaa tggcgtttgg
    23641 gagatagcct ttacagagca tgcttacaat tttgtggaat gtgcccccat gcttgagcgt
    23701 ccgaactatg taaaagagca tggccatggt ttcaggctcg acgactatat ttcgctcatt
    23761 gaggatatga aggctgaggg cttgtcggtg aaactcggga tcgagttcga ttacatcgat
    23821 gagaccatcg aagatgtagc cgactttctg gaagagggac atccctggga tctggttatc
    23881 ggatcggttc actggatcgg agagtgggga ttcgatattg acccgactag ctggaacgat
    23941 gtggatgtgg aatgggcgta ttccgcatat ttcgaacgtg tatgtcgtgc ggcaaggagc
    24001 ggcgttttcg acgtgatagg ccatcccgat gtgattaagg tgttcggcgc taggttggca
    24061 acgggtttgg agggcccagg cgaggaagga cccttgagag gatactacga tgctttgaca
    24121 gcggcggccg catcgtcagg aacatgcctt gaagtctcaa gcgccggact ccggcgtccg
    24181 gtagaggaag cgtatcctgc aatgacgttg ctcaagatgg cttgcgagcg gggcgtgggg
    24241 attaccttag cttcggatgc gcatgaacct gaagaggtcg catcgcgtta tgatgaactt
    24301 gtgtcgtggg cccgagaggc cggatacact aagttgacca tatttgacca gcgcactgga
    24361 aggcaagcgg cgatggggta gggtgcgccg ggagcatgtg gtttgctgct gcagggggag
    24421 ttcccagatg cttgttatcc tcagccagtt gcagaccctg atttttctcc gcccactggc
    24481 aaaatcctta gctgccagag ctaagtagac cgcaacaaat ctcttcgcga tcctcgagtt
    24541 gtactgccgc tgtctgggac ctaggcgggg aactgctaag gtcttttact tgtaaatgta
    24601 gccttcggta gaagcctagg gaagcgccgt gcggcgtgga gccggccaag gtttgccgcg
    24661 ggggcgagca ggttttgcag accagtctaa aacttagaca cccgcgcccg aaaagcgact
    24721 aaataataga catattcctt tgaatgcata gac
//
LOCUS       DAOSYL010000034        24719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1665_length_24719_cov_7.954711, whole genome shotgun sequence.
ACCESSION   DAOSYL010000034 DAOSYL010000000
VERSION     DAOSYL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_24719_cov_7.954711"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..362)
                     /locus_tag="PLU15_06850"
     CDS             complement(159..362)
                     /locus_tag="PLU15_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30678.1"
                     /translation="MGHKDIGVSIFAADAVPCSRRAILREVGRESGALPISRAGLDNS
                     YTVGGNALKGLNNFRPVQEFLSW"
     gene            417..3338
                     /locus_tag="PLU15_06855"
     CDS             417..3338
                     /locus_tag="PLU15_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOO30679.1"
                     /translation="MLEERYDVVVRRDAIDLLANDILDMIDDDSVMVIDDYHLVGGNP
                     EVNELVERLINTAPPHFHVVLSTRERPSFGSLARWRVKGEALEITEADLAFTNDEISA
                     LFTGVYDYSITPHQLTAISERTEGWIIALEMTWHALREGASFEDLITRPAASLTSVFD
                     YLAQEVLVKQAPYVVEFLIDVSVLDELDPAICNHVSGRDDSDSILAGLSSSGLFVYST
                     RTGAYRFHNLLREHLRRICRKDPGRWAAANQRAADYYGERGQSLQAVDCLLAADDWNA
                     AAQRLSNVAPELVTSGRYDALAARLSDLPGTVLEAYPDLLVYRADVYRLTSDYQNALS
                     WYSMADEIFAAKSDNTGRVRALKGKAMIYLDTVQPAKSEELLSEAFGLVASDDRLEQA
                     ALLRMMAETKTNEGQIQAAVEFARSAEQLINQAMEDELDIRVHLRTGRLSSALDSLRR
                     RVDKESGSFRPSRSHRETQLLLSLIYSLIGEGDAARSCAEAGIALGRDLKAPFVEAVG
                     YMRLGHACYISPYLGAPDAEECYRRALEITDEIGVVRGRAEPLMGLALLHAARGEAAL
                     AETEAREAMEICQAAGDEWLAGFVQLGVGSGLASSGNDLQARAWLETADETFARCGDE
                     YCATVCSMWLAVLALRGQENEERLTGILEILFRDVKSHSYEFLFTKRTLFGPPDLGAL
                     APLLIEASRLGVDREHVDWIRSVTGLPELEYHPGYTLHIRTLGRFALYRGTEEVSNRE
                     WQREKARSLFQLLVVNRKAFLHRDRILDALWPGLDSDIAARHLKVAFNTMLNVLEPDR
                     PPRLTSFCVTREGPLYGLNPHSGCWVDADEFESLTIKARRLAETDPEAARDLYRSALD
                     LYRGDFLPECLYEDWATVERERLLALYLRSAQSFAGLLFSGGETDECINVCEQVIAKD
                     VCWEEAYRLLILCYLQRGNKSMALRVYERCETTLKDEMGVGPSRDTMAIFQRVRMMPE
                     R"
     gene            3603..4376
                     /locus_tag="PLU15_06860"
     CDS             3603..4376
                     /locus_tag="PLU15_06860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO30680.1"
                     /translation="MQSGTPYGRVFAEVYEKKWTWFARQLAPKIESFYAATPIGRCNK
                     SILDVCCGTGQLALYFLERGFSVAGVDFSEHMLAHARAAAAEWIETGKARLVQANATS
                     FDVGAGYGLAVSTYDSLNHLPDIDALSACFRCIYRSLADDGWFLFDLNTDLAFRTDWN
                     GVSVQDDEESMIVMRSIYDSVNRRAFMKLSGFVRAQDGRYDRFEEFVSETAFPLKEVK
                     HALEDAGFSSVRCVDAADFGTPLDNDPETKKRVFLVAGK"
     gene            4482..4841
                     /locus_tag="PLU15_06865"
     CDS             4482..4841
                     /locus_tag="PLU15_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30681.1"
                     /translation="MREQRFVIRAAAVLDIGRGLPPAAQQTPRGLPAQFLTDCDTREL
                     RHDAQFEVKVHDGVYGVGACQFIGTLYEAIQLSQVRNSTFATGIIQILGIGDKAGASQ
                     ARLSRSKDRLWPLCLDV"
     gene            5389..5925
                     /locus_tag="PLU15_06870"
     CDS             5389..5925
                     /locus_tag="PLU15_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO30682.1"
                     /translation="MELWDVYDSNREKVGKTAVRGQGLEAGEYHVVVHIWLVNSEGNF
                     LIQKRAASVEAAPNIWATTGGSALAGENSAAACARELYEEIGVRADMGKARLAFTEKR
                     ADCFCDIWIIRQSINIEDCKLQVEEVSEVKWASPGEIRFMIAQGTFWGYRYIEGLFSL
                     VQADESESRPRKRSGFNS"
     gene            7183..7923
                     /gene="fabG"
                     /locus_tag="PLU15_06875"
     CDS             7183..7923
                     /gene="fabG"
                     /locus_tag="PLU15_06875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOO30683.1"
                     /translation="MRLDGKVAIITGSGRGLGKEAAFLFAEEGAKVVVCDINRQSAEE
                     VALAIVDAGGEAMATMVDVTDSESVQRMVDAAMERFGTIDVLVNNAGITADAQLVKMT
                     EDQWDRVIAVNLKGVYLCTKAVAPIMIAKGSGRIINTSSVVGIYGNFGQSNYVATKAG
                     VIGMTKVWAKELGRKGITVNAVAPGFTMTEMMSTVPEKVLTGIRDRTPLGRLGEPRDI
                     AYAYLYLASNEASFVNGATIQVDGGLTL"
     gene            7964..9256
                     /locus_tag="PLU15_06880"
     CDS             7964..9256
                     /locus_tag="PLU15_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOO30684.1"
                     /translation="MDVKELYRSKLISTQEAVGMVCSGQSIVSAMCAAEPPGLLGAIS
                     SRKCELENVTIMSCLLMRDYDFLKPEMKGHFLNEAWYYGQYSYMAHPGGTVTFLPNHL
                     SECGLKKAQIDPPDIFWGTATPPDKNGYMSLSLSLTYEKPMIAAAKTVVLEINENLPR
                     TLGDTHIHISQVDYVVENAVPLVEIDHITPSEVEMEIGRNVASLIEDGSTIQLGIGGI
                     PNAVTASLMDKRDLGVHTEMLTDGMVDLFNAGVVTGRAKTLWPEKMVGCFALGTRKLY
                     DFIDDNLGVELHGGHITNDPRVIAKNHKMVSINTTMQVDLAGQANSETLGTRQYSGGG
                     GALNTCMGAQMSPGGKSILALRSTAKGGAISTIVPCFGDGQSVTIPRADIDYVVTEYG
                     IARLKAMNIRKRVEALIAIAHPNYRDWLWSEAEALQYV"
     gene            9296..10300
                     /locus_tag="PLU15_06885"
     CDS             9296..10300
                     /locus_tag="PLU15_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOO30685.1"
                     /translation="MERYARIIGTGLYLPETEISNAHMAERINQAAPGLGDVVFKFEE
                     SSRIMKRYYAPRDWATSDLAAKAAMAALEDAGVAPEDLDLIILGTDSPDFITPAMSVV
                     TQHKIGAKNAGTYDIGCACASFPTGIAAAAGHIATNPWIRYVLVIGAYMMSKLADPND
                     VTSFFYGDGAGAAVLTVDDKPGFITSSFIADGSYHEAWGIYSGGTYEPATCDSVQAGR
                     TNVRFVSKYPAEVNHQGWPKVVRALAERGGFEVSDIDLAIFTQVNWYSIDIVMADLGL
                     PIEKAHRIMDRMGYTGSACIPMALDDARKQGRINPGDLVVLVGSGVGYNQAGVALRMW
                     "
     gene            10501..11679
                     /locus_tag="PLU15_06890"
     CDS             10501..11679
                     /locus_tag="PLU15_06890"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOO30686.1"
                     /translation="MREVVIASAARTAIGTFGGTLATVPAPRLGTIAVKAAIERAGIR
                     NDAVDEVIMGCVLQAGQGQNPARQAAIGAGLPVEIPAMTVNKVCASGLKSVALAAQAI
                     MVGDAEIVVAGGLENMNMAPYILDKARTGYRMGNAEIIDEMVHDGLWCIFGNTHMGIT
                     AETVAEKYGISREEQDEFAAKSQQKAAEAIEAGRFAEEIVPVQIPQRKGDPIVFAQDE
                     HVRPGTTVEVLAKLRPAFKKDGTVTAGNASGINDGAAAVVVMSAERARDLGIEPLAKI
                     RSYASLGVDPSVMGIGPVPATRKALAKAGLSLEDIDLIEANEAFAAQSIAVGRDLAWD
                     PDKVNVNGGAIALGHPIGASGTRILVTLLHEMKRRDAKLGLATLCIGGGMGMAMIVER
                     "
     gene            11898..12119
                     /locus_tag="PLU15_06895"
     CDS             11898..12119
                     /locus_tag="PLU15_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30687.1"
                     /translation="MTRETVIASFVIRFVKPAVSMGPLERADMDLDDGAPNLRIAVRH
                     IQGGYERRFADLDDAIGFIRDEIQLLELE"
     gene            12132..13001
                     /locus_tag="PLU15_06900"
     CDS             12132..13001
                     /locus_tag="PLU15_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO30688.1"
                     /translation="MFGQQVVSGLSAGCLYALAALGLVLVYRTMEIVNFAHGEMAMVS
                     TFVAHTLLIKAGLPYILAMIGALIFAFIFGMAVERIFLRPIQEGPLISMMIMTLGLFM
                     VFNGAAGWIWGFDPVSFPYAVKGRPILIGDIIVTRDSLLVLAVTVTIAGVLYAALKFT
                     MAGIAIRAASQNPRAARLMGVPVPKVYSLTWGISAVLGAVAGILIAPTTFLSPSMMAE
                     VQIKAFTAAVLGGFSSLPGAVVGGLLLGVLENLVAGYISTQLKSTFAFALIVIVLLIR
                     PSGIMGATEKRKV"
     gene            12998..14029
                     /locus_tag="PLU15_06905"
     CDS             12998..14029
                     /locus_tag="PLU15_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO30689.1"
                     /translation="MRLVKDTPKGTRCLLKMPGGRSVLLGAALLLLPLVTMAFGRTYV
                     VYLYSLVMVYVIVSLGLNILTGYTGQISIGHAGFMGIAAYASAIFTGRLGIPFVPAMM
                     LSGLLAAVIGLGLGVPALRLHGHYLAICTMGFGVAVNQLSAVWDGLTGGYQGMKVPAA
                     SIFGYRFESDLSYYYLALAVTWLMVVAARNILRSRPGRALMAVRDSEVAAEAMGINLA
                     KYKVAAFAISAFYAGIAGSLYAHLVKYVSPYDFNMGVSMTLLASICVGGLGSVPGSIL
                     GAIFMTLMPHAFSRVKNLPLVLSGISLILVVLFLPYGLASLPWKIKSARSARSTGGTA
                     GRVETSGDA"
     gene            14016..14795
                     /locus_tag="PLU15_06910"
     CDS             14016..14795
                     /locus_tag="PLU15_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30690.1"
                     /translation="MAMLELEGITVRFGGLVAVDSLSFGVREGTIHGLIGPNGAGKST
                     VFNCISGFVRQDEGSISFRGQRLACEPHDVIARGIARTFQNVELFRGQTVLDNVLIGL
                     HTAGTANFITGAFQTRSARKDEERLREQAMKTLDFLGIGEYAGRIAYGLPYGVQKLVE
                     LARALALRPNLLLLDEPVAGMNPTERGELAEIIRNIKKEFGMTVLLVEHDMSFVMGLC
                     DEITVMNFGRKIAEGTPSEVQQDPQVIQAYLGEDAGDAEAI"
     gene            14779..15522
                     /locus_tag="PLU15_06915"
     CDS             14779..15522
                     /locus_tag="PLU15_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30691.1"
                     /translation="MLRLSDVDVYYGMVGALRNVSMEVRQGEIVALLGANGAGKTTTL
                     RAISGIVRPASGSIEFEGRAIHGLSPERIVSLGIVQAPEGRQVFPELSVKENLLIGTY
                     SRRDRAKIPEDFAHVYDHFPVLKERESQPAGTLSGGEQQMLAIGRALMASPKVLLLDE
                     PSLGLAPLATKQIFKIIEALNAEGTTILLVEQNARLALAVSNRAYILQTGSVALSGPS
                     DELLASEDVKRSYLGGSTGGRDIAEVERA"
     gene            15752..16948
                     /locus_tag="PLU15_06920"
     CDS             15752..16948
                     /locus_tag="PLU15_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30692.1"
                     /translation="MKRHWTLVLASLMVVLLMGIPGPVTAAPEPGVTANQVLIGAFQD
                     QSGPAAVVGINMRKGMEAYFNWINSKGGVNGRKIRFIVEDDGFQPSRSIAAAKKLIEQ
                     DKVFALVGTLGTPGVQATIDYIQSKKVPSIYQGSGISELAFPPKRYFFPVQPNFISEG
                     RIIAQYVVEGLKCKRIAVACEQTDIGSEGLRGIKEQLAVYKMQPVEVVNFGAADVDFS
                     SQVLKLIKAKPDAVIIYSTLKPCAGFLKQADLMGLDAQFLTTYVNADPIQMPALAGEA
                     CVGLIAPGWVPVLGNDEDSVKYLEIYQATYPNEMPSAFAAAGFIAAEVFCEGLRRCGA
                     NPTREGLVAAMETFRDWNGIMAKGITYTPEWRSGKASMYFMRFAKPKFEEDSIEVISG
                     WVKLKI"
     gene            17585..18541
                     /locus_tag="PLU15_06925"
     CDS             17585..18541
                     /locus_tag="PLU15_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOO30693.1"
                     /translation="MPDKASGQPVVLEYVKMRLPELSALDRESTVFIISVSPIEVHGP
                     HLPVGTDVFIAEELAARYIRELFARHPELTFVTLPSLYLGSDALPVKGSLSVPAPYLE
                     GALFSYGKGLAAQGFRYLFVADNHGGPRHQMAIEAAARALWKKHKFYLIDPFNIEFRY
                     MVEHDPDFVKRTGLGPGVCGDDPDSHAGTNETSLMLACCEEQVAGGYREVPASLPPPH
                     TGGASFVAGLAKIVRFFGGDVLSRDLEHLANTLAWVGDPDMLPYMGDPSKATVEAGEA
                     MLAARVEVAMELFERALASGGKDPVHLEPMLWGVRAMRKLPE"
     gene            18767..21319
                     /locus_tag="PLU15_06930"
     CDS             18767..21319
                     /locus_tag="PLU15_06930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO30694.1"
                     /translation="MEICFLGGAEEVGASCILVKTESGKNVLLDAGVRVSEEGTEMLP
                     NLEPLNDVVIDAIILSHAHLDHSGALPLVAKLSPGASIFATTATRDLATVLLYDSVKV
                     AEFREGLQLFDADDAQQALDRVVTYGFGTSFEAVEGIQAVFLQAGHILGAAAVLLQTE
                     EGTLLYTGDFTTFEQETVGMQAFSGALKRGADVVITEATYGSRIHAPRSQEMQRLLDT
                     IGETLDAGGRALVPAFAVGRSQEIILALRNYMRRTKRKYPVYVDGLIRNVNTVFARNP
                     NYLKQRYKKEVLRGHELFYTNGIEAVGSKSQRDKILASKDPCVIIASSGMLTGGVSPI
                     YAERILEDEKNLLAIVGYQDEESPGRRLLDLAELPKNERTVLLNENEYRVRCKVGKFG
                     LSAHADSLGIIASVKALRPDFVLLNHGNEESLEALSQALADELPDTRIEVAGSGGVYE
                     YVPAPGGGKRRYSLKPQVRRIALHKEEPVDPEELWQHLFQTGTLGTTLTVADLMMVWY
                     GPREISDEEKRAFRKEVSGSPRFRSAYTNPNIVYVLTEEEYVDAVTPKPMEQNEAWAL
                     IQERLSPFGLQKVGFASDGSVTLYFPTPKYAERCTDEIGELEREILRRIEVAPNSNME
                     FLKTKIKTDLAEEFGIRVNRDPSFGAQSVTVKEPAGLAQPGIRGRTLGEYAEAFEEET
                     GYKLLFRVEGQSLLDQVAARRSQDPWTPEGVPSHGVSEPMEQNMAKGMIDDAFAGAVN
                     QPKVSIYRDEGRMALAFITPQVGERYKEILDKLEAATGWRLEIQESARVNELADLARE
                     LLAANGLGGMKVGVHSGYAEARGPVDVDEAVAQRLSKAYSELTGYELRFRRT"
     gene            21384..23009
                     /locus_tag="PLU15_06935"
     CDS             21384..23009
                     /locus_tag="PLU15_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO30695.1"
                     /translation="MKAVYGSALLTKPRPSWTLTMAWALVAAALSGHAAAGEIGAELV
                     LSPQARAIVETNRPICPGPSGDDIEREAIELLYNYTGLEDEIDVAQIWRNQGRSVRTG
                     QSHQWHISPHYAAEEVELLFEVLKYGYAGYEYFGGDEAFEAAKDGILREIGEFAEEIP
                     MWQYIDIIAANLSFVRDGHLSIGERRICEYHQFRASPTHSFCMDNEGQFWNADGERIL
                     SIDGQSPSAFMKPSIDEAGDIVYLLGMVLPEGNGDVALKLEYADGCDEMAWLVPFSPM
                     VLDGPVYELSEVGGVPLITCGDFRDLPGQRRAIARFAEDAEGFRDEDVIILDLRSNRG
                     GRYAYGREWCRRLSGGDVGPSTYSRRLVTDVALALLSNTLSESDDNLSSLENQDDAPW
                     MKDRSEDPELPGWSYAVVEQEARAGRTPFVVVLLDSYVASAAEVFVRRLRYCQNTVFI
                     GTNTRGAALCGNAGVMELSYSGLKVSLPTMICFDGEMQNIDGCGYVPDFWVHPDFVME
                     RAVRFIEKQLVSVQADGALGQTSEEEPGGIMRQ"
     gene            23052..24626
                     /locus_tag="PLU15_06940"
     CDS             23052..24626
                     /locus_tag="PLU15_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO30696.1"
                     /translation="MRNPLHLRESRLFQALMAFCVIAIVTPVQPVLASSRTRLVLSPE
                     AKAIVEANRPELSEASREDLLARFSSMLYEYSDFEDEAYPSALRWDTAAGRESVMEEW
                     SISPARAVQEVDLLFHALKYGYAAYQFFGGDEVFCAARERMLDEIGRHYHDIPFWQYS
                     QIIIANLKFIQDGHFAFGSRTLCEWSTFRVSPYLRFSLDEDGFFINDGGKRVASVSGQ
                     APSSFLKPSIDEDGNIVYVLGMLVPEQQVDLMCEIEYADGSREIAWLVPIISMDYGGA
                     VYELGEIEGFPLVSCRSFDPDHDEGKALNRFVEDAEKLRQEDVILLDLRSNHGGSTIY
                     GMLWCQRLTKGNVGPYFFTAELATDTSIALLLNGLSERLGEEFSSDQAEALWRYWKNA
                     EDPDRPGWRRIGIEQGGHISDAPFIVALVDSNAGSATEAFVRRLRSLDNVVIIGTNTR
                     GALLCGNPGMLVLPYSGFEVRIPTMFWLDSEMHNYDGVGYSPDFWVHPDYALERAVKF
                     IRRHLVREGASVSLGG"
BASE COUNT         5584 a         6204 c         7594 g         5337 t
ORIGIN      
        1 cccgctgggc gcgggcggct gccgttggaa gaagacagag cgcgttagaa cggaaaaacg
       61 gatgcgagga ggaaagcggc gtctgcagcg gcagctgccg gcgcagaagc gacatggtct
      121 atagccctcc gatagttcga accaaattcg tgccgccgtt accaagaaag aaactcttga
      181 acaggcctga aattattgag gcccttcaag gcattgcctc ctaccgtgta actattgtcc
      241 aggccggccc gggatatggg aagagcaccg ctctcgcgtc ctacctcgcg caaaatcgca
      301 cgcctgctgc atggtacagc gtcagcggcg aagatgctga ccccaatgtc tttatggccc
      361 atctcatctg gtccctccgc gctttgtggc ccgccatcgg ggggcggtcc ttgtcgatgc
      421 tggaggaacg atacgacgtt gtggtgcggc gcgatgctat cgatcttctt gcaaacgata
      481 tcctcgatat gatcgacgac gactctgtca tggtcatcga tgattaccat ttagtgggcg
      541 ggaatcccga agtcaacgaa cttgtcgaac gcctgatcaa caccgctccg cctcactttc
      601 acgttgtcct ttcgaccagg gagcgcccct cctttggttc actggccaga tggcgcgtga
      661 aaggcgaagc gctggagata accgaggccg acctggcttt caccaacgac gagatatccg
      721 ccttattcac cggagtctat gactattcga tcactcccca tcagctaact gcgatatccg
      781 aacgcaccga aggatggatc attgccttgg agatgacgtg gcacgctctg cgcgaaggag
      841 catcattcga agaccttatc accaggccgg ccgcctcgct gaccagcgtc ttcgactacc
      901 tcgcccagga ggtcctcgtg aaacaggcgc cctatgtagt ggaattcctc attgacgtct
      961 cagtccttga cgaactggat ccggctatct gcaaccacgt ctcgggcagg gacgacagcg
     1021 acagcatcct tgccgggttg tcttccagcg gactcttcgt ctactcgaca agaacagggg
     1081 cgtacaggtt ccacaacctc ctcagggaac atctgcgccg gatctgtcgg aaggatccag
     1141 gaaggtgggc ggcagcgaac cagagggcgg ccgattacta tggagaaaga ggccagagcc
     1201 tgcaggcagt ggattgcctg cttgcagcgg acgattggaa cgccgcggcg caacgcctgt
     1261 cgaacgtggc gcctgaactt gtgacgagtg ggagatatga tgctctcgct gcgaggctca
     1321 gcgatcttcc cggaactgtc ctggaggcct atcccgacct attggtttac agagctgacg
     1381 tttacaggtt gaccagcgac taccagaacg ccctgtcatg gtattccatg gccgatgaga
     1441 tctttgcagc caaatctgac aatactggaa gggtgcgtgc tctcaagggg aaagcaatga
     1501 tctatctcga tacagtccag cccgcaaaga gcgaggaact gctttcggag gcttttggac
     1561 tcgtcgcttc cgatgacagg ctggaacagg ccgcccttct caggatgatg gctgaaacta
     1621 agacaaatga aggtcaaatt caggccgctg tcgaattcgc gcgctctgcc gaacagctga
     1681 taaaccaggc aatggaggac gaactggaca taagggttca ccttcgaacc ggtaggctct
     1741 cgtctgccct cgattccctg cgccgcaggg tagacaagga gagcggatct ttcaggcctt
     1801 cccggtcaca cagagaaaca cagctccttc tctcgctgat ctactcactc ataggcgaag
     1861 gcgatgccgc tcgcagttgc gccgaggctg gaatagccct cggacgcgac ctcaaagctc
     1921 cctttgtcga agccgtaggc tacatgcgcc ttggtcatgc atgttatata agcccgtatt
     1981 taggcgcccc tgatgcagag gagtgctaca ggcgggcact ggagattacg gatgagatag
     2041 gggtggttcg gggacgggcc gaacccctca tgggccttgc gttgctccat gccgcaaggg
     2101 gcgaggcggc gctggcagag acagaagccc gggaagcgat ggagatatgc caggcagccg
     2161 gcgatgaatg gcttgccgga ttcgttcagc tcggcgtggg ttcgggcttg gcctcgtcag
     2221 gcaatgacct gcaggcccgc gcctggctgg aaacggccga tgaaacattt gcaaggtgtg
     2281 gcgatgaata ctgcgcaact gtctgcagca tgtggcttgc agtattggcc ctgcgcggcc
     2341 aggagaacga ggagcgtttg acggggatcc ttgagatcct tttcagagat gtgaaatccc
     2401 acagctacga atttctcttc accaagagga cgcttttcgg cccgccagac ttgggggcgt
     2461 tggcgccatt gctcatagag gcgagccgcc tcggcgtcga cagggaacac gtggattgga
     2521 tcagatcagt gactggcctt ccggaactgg aataccatcc tggttacact cttcacatta
     2581 ggacactcgg caggtttgcg ctctatagag gcacagagga ggtttccaac cgagagtggc
     2641 aaagggagaa ggcgcgaagc ctcttccagc tgctcgtagt aaacagaaag gcattcctcc
     2701 acagggaccg aatcctcgat gcgctctggc ccgggctcga ttcggacatt gctgctaggc
     2761 acctgaaagt ggccttcaat acgatgctca acgtgctcga acccgacagg cctccgcggc
     2821 tcacttcatt ctgtgttacc cgcgaggggc cgctctatgg gcttaacccg cactcaggat
     2881 gttgggtgga tgcggatgaa ttcgaaagcc tgaccataaa agcgaggcgt ctcgccgaga
     2941 ccgaccctga ggccgcccgc gatctttatc ggagcgccct cgacctttac cgaggggact
     3001 ttcttcctga gtgtctctat gaggactggg cgacggtcga gcgcgaacgg cttttggccc
     3061 tgtatcttcg ctcagctcag agcttcgcag gactcttgtt ctcaggtggc gagactgatg
     3121 aatgcatcaa cgtgtgtgag caggtgattg cgaaagatgt atgttgggag gaggcctatc
     3181 gcctcctgat cctctgttac cttcagcggg gcaacaagtc catggcactc agggtgtatg
     3241 aaaggtgcga gacgacgctc aaagacgaaa tgggggtagg gcctagccgc gacaccatgg
     3301 caatattcca gcgcgtcagg atgatgcctg aaaggtgagg agagtgggct aatacaggat
     3361 ttgggatgcg ggcgattcgg gctttcaggc tgtggcgtcg aggttcattc tctaaaccga
     3421 caaaaacgag cggcgatcaa agaagatacc ttgtccatta agaaaactgt gacatagccg
     3481 atttcgtgtt gccggcacat actgtggcgg gccccgaacg tgctcaggag gggaataggg
     3541 tagcctgcga gtgattgtgt tcgggcaaat ccggtcgcag taagattgga ggaagcgcag
     3601 ctatgcaaag cgggacgccg tatggtcgag tattcgcaga ggtgtatgag aagaagtgga
     3661 cttggtttgc gcggcagctg gcgcccaaga tagagtcgtt ctacgccgcg acgccaatag
     3721 gaagatgcaa caagtcgatc ctggatgtct gttgtggcac cgggcagctt gccctctact
     3781 tcctcgaacg cgggttcagc gtcgctggtg tggatttttc cgagcacatg ctcgctcatg
     3841 cccgcgccgc ggcggcggaa tggattgaga ccggcaaggc gcgcttggtt caggctaacg
     3901 cgacgagctt cgatgtgggc gcaggttatg gcctggccgt ttcgacatat gattctctca
     3961 accatcttcc cgacatcgac gccctttcgg cttgttttcg ttgtatctat cggtctcttg
     4021 ccgacgatgg atggtttctt ttcgacctca atacggatct ggcattcagg accgactgga
     4081 acggggtttc ggttcaggac gatgaggaat caatgatcgt gatgagatcg atctacgact
     4141 ctgtgaacag gcgagccttc atgaagctgt cggggtttgt gagggcgcag gatggcaggt
     4201 acgataggtt tgaggagttc gtgtctgaga cagcgtttcc tctgaaagag gtcaagcatg
     4261 ctttggagga tgcagggttc agtagcgtga gatgcgttga cgcagcggat tttgggacgc
     4321 cacttgacaa tgatcccgag accaagaaga gggtattttt ggtggctggc aagtgaaagc
     4381 ccggcgtcag gccggcagac gacggtcggt aagtaagatg agggctctgc ggagagccta
     4441 agataacaaa aggaggagtg tgttgaggaa ttaggagatt cttgcgagaa cagcgtttcg
     4501 ttattcgtgc tgctgccgtc ttggacattg ggcgtggatt gccgcccgca gctcaacaga
     4561 ctccgcgagg cctgcctgct cagtttctta cggactgcga tacccgcgag cttcgacatg
     4621 atgcccagtt cgaagtgaag gtccatgatg gtgtgtatgg tgtgggtgcc tgtcaattca
     4681 tcgggaccct gtacgaggcg atccagctct ctcaggtgag gaattctacg ttcgccaccg
     4741 gcattataca gattttggga atcggggata aggcgggagc atcacaagcc agattatcac
     4801 gtagtaagga tcgactgtgg ccgctgtgcc tcgacgtcta gctggatcaa ctatcttctc
     4861 aatgatgatt gtgaagggca gggtttgtat tcttagacag gaaatcggca ggtcatggtt
     4921 tcaaccgcat caagcacgaa gcatactact acattgtgga cctcactcac tacctactcg
     4981 agcttgggca gaaaaacacg ccggagtctg tcgattggga ggattacatt cgcagcgaca
     5041 ttctgctttg aaatgtgtac gaggtggttt cgcttgaaga gtacatatac cacttccggc
     5101 tggaaaggga tttgcgatat gctattgcct tgtctgtagg cttcacggct ccggacgcgt
     5161 ctcccgttgc ccacaagtat ccgggtgggc gtcttgaagt aacattccct gaagggttgg
     5221 agctgagggt attgtatggt gaccctgatg acttcgcttt tgccctcgag ccggcaaaaa
     5281 cgactaggag gaactttcgt cagcaggctt tgcaatgccg cagccctgac gtgggaggcc
     5341 aattgtggta gagtagttgc agtctgtttt gagaggcggg gcgcattcgt ggaattatgg
     5401 gatgtatatg actccaacag agagaaggtt ggcaagactg cagtgcgagg gcagggactt
     5461 gaggccggag agtatcatgt tgttgtccac atatggttgg tgaatagtga aggcaacttc
     5521 ctgatccaga agcgtgcagc atctgtcgag gcggcgccta acatctgggc cacgaccggc
     5581 ggatcggcgc ttgccgggga aaacagcgcc gctgcgtgcg caagagagct gtatgaggag
     5641 atcggagtca gggccgacat gggtaaggcc cggcttgcct tcacagagaa gagggcagac
     5701 tgtttctgtg acatttggat catccgacaa agcatcaaca tagaagattg caagctgcag
     5761 gttgaggaag tgagcgaggt gaaatgggcc tctcccggag agatccggtt catgatagcc
     5821 caggggacgt tctgggggta ccgatacatt gaaggcctgt tcagcctggt gcaggctgac
     5881 gaatccgaaa gtcgcccaag aaagcgttcc ggcttcaaca gctaatacag gcgtcggccc
     5941 catctttgcc gctcccttca tggaatgctg acacatactt ggatcatctt ggcgcttggg
     6001 gcccgttaaa agcccagcat agcccgccaa tctggaacag atcgactcag caggtcgccg
     6061 tgcggtggga aaacgaagaa gaaggcgctg cggtgatagt cggaatactc tgaggctggg
     6121 aaagccagcc acattgggaa gcggcgaggc tccacaaagg ctaaaacgcg aggcctcttt
     6181 ccacctgctt cattctgcac tgcatacata aaaaagtaga cgatgcgggg acagcattcc
     6241 tcatgtccgg cagttcagtg ttgccaatca aggctgcggt tgtcgtaagc gctagagtct
     6301 tgcgaggcca cgagcgtgct cgcgctgtag ttcttcaagc attgatcgaa ctagccctgc
     6361 tccgaaaagc atggacctgt atgctgaaaa tcggctgatg cgacttttcg cgaatatccc
     6421 ttccaatccg cttcggccca cctgagtaga ccgcaacaaa tctctgcgcg atccccaagt
     6481 tgtactgtcg ctgtctggga gactcctaag cctaggaaag cgccgtgcgg cgtaaggttc
     6541 gccgcggggg cgagcaggtt ctgcagacca gtctaaaact tagacacccg cgcccgaaaa
     6601 gcgactaaat aatagacaca ttccttgaag gcaggcccaa gtcgcccttt catatgggtg
     6661 cgttgaatgc ataaactgct ggagcgctca cacggatctt atgcgcatcg ctttcgtcgt
     6721 tgtaaagaag atggttgccc gcaagcgtcg aaccgggatt tcgattacgc catctaccct
     6781 ggctttgccc cgtgaatcat atcccggcgt ggctcaatgc tgcgggcgtc tatggctaag
     6841 gccaagatgc gtacgttgtc gatgcacaaa tgcgaaccaa ttaccgcact gcgacatcgg
     6901 tgcaaagacg tgtctacacc tatcagacat gatatggcag gccgcaaaaa actattggga
     6961 gttacttagc atgcctgtag cttgccacat cttgaagccc tattccaaag acgctcaatc
     7021 ggaaacaggg cacgaaaagt tgttacccgt ctgtaacttc cccgtaacct attatcatta
     7081 ccatcaattc agagtctgtg tactccgctc caatgacaca gcgaggcacg gaagataccg
     7141 tagtaccgga atattgcgga ttgcggggag gagcagaaga gaatgcgact tgacggcaag
     7201 gttgcaataa tcacaggatc cggaaggggt ttaggcaaag aagccgcttt tctttttgcg
     7261 gaagagggcg caaaggtagt ggtctgcgac atcaaccgac agagcgctga agaggttgcc
     7321 ctagccattg tcgatgcggg cggcgaagcc atggccacaa tggtggacgt gaccgactct
     7381 gaaagtgtcc agaggatggt ggatgccgcc atggagcggt tcggaaccat cgacgtgctg
     7441 gttaacaacg ccggcataac cgccgacgcc cagctggtga agatgacaga ggaccagtgg
     7501 gatcgggtga tcgccgtcaa cctcaagggc gtgtatcttt gcaccaaggc ggtggcgccc
     7561 attatgattg ccaagggttc gggcaggatc atcaacacct cttcagtggt gggcatctat
     7621 ggcaacttcg ggcagtccaa ctacgttgcc accaaagccg gggtcatcgg aatgacaaaa
     7681 gtctgggcca aggagctcgg acggaagggg atcactgtaa acgctgtagc ccccggtttt
     7741 accatgacag agatgatgtc caccgttccc gaaaaagtcc tcaccggtat cagagacaga
     7801 actcccttgg gcagactggg cgagcctcga gacatcgcct atgcatacct ttatctcgca
     7861 tctaacgaag caagttttgt caatggcgcg acgattcagg tggatggcgg tctgactctt
     7921 tgatcacgat ttggcacaag acatgttgcg aggagggacg gtaatggatg ttaaggagct
     7981 ttaccgcagc aagctgatct ccacccaaga ggcggtgggc atggtctgtt cgggccagag
     8041 catcgtttca gccatgtgtg cggcagagcc cccgggactt ttgggggcga tctcttcccg
     8101 caagtgcgag ctggagaatg tgaccatcat gtcttgcctc ctgatgcggg attacgattt
     8161 tctcaagccc gagatgaaag ggcatttcct aaacgaggcc tggtattatg gtcaatactc
     8221 ctatatggcc catccaggcg gaacagtcac gttcctcccg aaccacctca gcgagtgtgg
     8281 gctcaagaag gcccagatcg atccgcccga catcttctgg ggaactgcca ctccgcctga
     8341 caagaatggt tacatgtcct tgtccctcag tctcacctat gaaaaaccca tgatagcggc
     8401 ggcaaaaacc gtcgtccttg agataaacga gaatctccct cgcaccctag gcgacaccca
     8461 catccacatc tcgcaggtgg actatgtcgt cgaaaatgcc gtccccctcg tcgagataga
     8521 ccacatcacc ccttcagagg tcgagatgga gataggacgt aacgtggcgt ccttgatcga
     8581 ggacggaagc accatccagc tggggatagg tggcattcca aatgcagtga cagcatctct
     8641 catggacaaa cgcgacctcg gagtccacac cgagatgctt accgacggca tggtggacct
     8701 cttcaatgcc ggggtggtta cagggcgcgc gaagacgctg tggccggaga agatggtcgg
     8761 ctgttttgcc ttgggcactc gcaagctcta cgacttcatc gacgacaact taggagtgga
     8821 gcttcacgga ggccacatca ccaacgatcc tagggtgata gccaaaaacc acaagatggt
     8881 gagcatcaac accaccatgc aggtggacct cgccggccag gccaactccg aaaccctggg
     8941 aacgaggcag tacagcgggg gaggcggtgc cctcaataca tgcatgggtg cgcagatgtc
     9001 gcccggaggc aagtcaatac tggcgctgag gtctacagct aagggcggtg ccatatccac
     9061 catagtgccg tgttttgggg acggacagtc agttacaatt cccagagccg acatcgatta
     9121 cgtggtgacg gagtatggca ttgccaggct gaaggctatg aatattcgga aaagggtcga
     9181 agctctgatc gccatagccc atcccaacta tagggattgg ttgtggtcgg aggccgaggc
     9241 tttgcaatat gtgtagcccg tggcggaaac taccgaatta acggaggcgt tgttgatgga
     9301 acggtatgcg aggatcatcg ggaccggtct ctatcttccg gagaccgaaa tatccaatgc
     9361 ccacatggct gagaggatca atcaggccgc acctggccta ggtgacgtgg tgttcaagtt
     9421 tgaagagtct tcgcgcataa tgaaacggta ctatgctccg cgcgattggg ccacaagcga
     9481 ccttgcagca aaagcggcaa tggccgcctt ggaggacgcg ggggttgccc ctgaagacct
     9541 ggatttgatc atcttgggca ctgacagccc agatttcata acccctgcca tgtccgtggt
     9601 aacacagcac aagatcgggg caaaaaatgc cggcacctac gacatcgggt gcgcctgcgc
     9661 ttcctttcct acaggtattg cggctgcagc tggccacatc gcgaccaacc cgtggatccg
     9721 ttacgtgcta gttatcggcg catacatgat gagcaagttg gccgacccca atgacgtcac
     9781 ttccttcttt tacggggacg gggcgggggc ggcagtgctt accgtcgatg acaagcccgg
     9841 gttcatcacg tcatcattca tagctgatgg atcctaccac gaggcatggg gtatctactc
     9901 tggcggaacg tacgaacctg ctacctgcga ctctgtccag gcgggccgga cgaacgtgcg
     9961 gttcgtgtcg aaatacccgg cggaggttaa ccatcagggc tggcccaaag tagtgcgggc
    10021 gctcgccgag aggggcgggt ttgaagtttc cgatatagac ctggccatat tcacccaagt
    10081 aaactggtat tccatagaca tagtcatggc cgacttgggc cttcctatcg aaaaggcaca
    10141 tcgcatcatg gacaggatgg gatacaccgg atctgcatgc attcccatgg ctttggacga
    10201 tgcgaggaaa caggggagga ttaaccctgg cgacctggtg gttctcgtgg gttccggagt
    10261 aggctacaac caggccggag tcgcactgcg catgtggtag cgggggagcc acgaactcca
    10321 gccgaatcgg gggcttgggg cgctgccatg gatggtattg ggactggttc cctaactgag
    10381 aaaggctggg cgggggtgga aggttcaggc ccaacttcgt tgaatcttgg aatgtgcagg
    10441 ggtctcggcg aatgctgcct tgaccttgtg cgaccagaat tgcaatggag gtatagaggc
    10501 atgagagagg tagtcatagc ttctgctgcg cgaactgcca taggcacgtt tgggggaact
    10561 ctcgcgactg tgcccgcgcc ccgtttgggc actattgcgg taaaggcggc tatcgagagg
    10621 gcagggattc gaaacgacgc ggtggatgaa gtgatcatgg gttgcgtgct ccaggccgga
    10681 caagggcaga accctgcgcg acaggcggcg atcggggccg gattgccagt ggaaattccc
    10741 gcaatgaccg tgaacaaagt ctgcgcttca gggcttaagt cggtggcctt agccgcccag
    10801 gcgatcatgg tgggtgatgc cgagatcgta gtggcgggcg ggctggagaa catgaacatg
    10861 gccccataca tcctcgataa ggcgaggacc gggtatcgca tggggaacgc tgagatcatt
    10921 gacgagatgg ttcatgacgg gctgtggtgc atcttcggaa acacccacat gggcatcacc
    10981 gcggagaccg ttgccgaaaa gtacggaatt tcccgcgaag agcaggacga gtttgcggcc
    11041 aagagccagc aaaaagcggc cgaggccata gaggccggca gatttgctga agagatagtt
    11101 cccgtacaga tcccccagag aaagggagat ccgatcgtat ttgcccagga tgagcacgta
    11161 aggccaggta cgacagtgga ggttctcgca aagctcaggc cggcctttaa gaaggacggc
    11221 acggtaactg ctggaaacgc ctcgggcatc aacgacggcg cggcggcggt agtggtcatg
    11281 tcggccgagc gcgcacgcga cctggggata gagcctctgg ccaagatcag atcctacgcc
    11341 tctttaggtg tcgatccgag cgtaatgggg atcggaccgg tgcctgccac cagaaaggcg
    11401 cttgcaaagg ccggactgtc cctggaagat atcgacctga tagaagcgaa cgaagccttt
    11461 gctgcgcagt ccatagcggt cggacgcgac ttggcatggg atcctgacaa ggtgaacgtc
    11521 aacggaggcg ctattgccct tggccatccc atcggcgcaa gcggaacgag gatcctcgtc
    11581 accctcctcc atgagatgaa acggagagat gccaagctcg gccttgctac cctttgcatc
    11641 ggcggaggca tgggcatggc gatgatagtt gagaggtaat ctctacaggc aatcactttg
    11701 ccgggtggtc tgaaagttcg gaggctcggg aacttccgag gcaagcctcg tcagctggcg
    11761 agcgatgcaa agcagagcac tgggaatttg agatggacgc gggtggggcc ggatgacgac
    11821 cctgcgcggc aatagcctct gggaaggacg gtcccgcatc tattacactt aacagaaata
    11881 gcggaggagt tcgagaaatg acgcgcgaga cagtgattgc gtcgttcgtg atcaggttcg
    11941 tcaagcccgc ggtgagcatg ggcccgctgg agagggcaga tatggacctg gacgatgggg
    12001 cgccaaacct cagaattgcg gtcaggcaca tccaaggggg atatgagcgc aggttcgcgg
    12061 atttggatga tgccatcggc ttcatccgag atgagatcca actgcttgag ctggagtagg
    12121 gggtggggaa tgtgttcggg cagcaggtag taagcggcct ttcagctgga tgcctatatg
    12181 ctctggcagc gttggggctc gtactggtct accgaaccat ggagatcgtc aacttcgccc
    12241 acggcgaaat ggccatggta tctactttcg tggcgcacac cctgctgatt aaggcaggac
    12301 tgccatacat acttgccatg attggggcat tgatattcgc attcatcttc ggtatggcag
    12361 tcgagcggat attcctcagg ccgatccaag aagggcccct catcagcatg atgatcatga
    12421 cactcgggct tttcatggtt ttcaacggag cggcgggatg gatatgggga tttgacccgg
    12481 taagctttcc atacgcggtt aagggacgcc ccattctcat cggggatatc attgtgaccc
    12541 gcgacagcct tctcgtgttg gcagtcaccg ttacaattgc cggagtgctc tatgcggcgc
    12601 tcaagttcac catggccggc attgctataa gagctgcctc acagaacccg cgcgccgcga
    12661 ggctcatggg agtcccggtg ccgaaggtct attcgctcac ctggggcata agcgcagttc
    12721 taggcgctgt tgccggaata ctgatcgctc caaccacgtt cttgagccct agcatgatgg
    12781 cggaggtcca gataaaggcc ttcaccgctg cggtgcttgg aggattctcg agcctgcccg
    12841 gggccgtggt cggcggattg ctcttaggcg tgcttgagaa cctggttgcg ggttacatct
    12901 ctacacagct caagagcact tttgcctttg ctcttatagt catagtcctc ctgatccgtc
    12961 ccagcgggat catgggcgcg accgagaaaa ggaaggtgtg aggctcgtga aagacacccc
    13021 caagggaact cgttgcttgc tcaagatgcc tggcggtcgc agtgttttgc tcggcgcggc
    13081 cctccttctg ttgcccctcg ttacaatggc tttcggcagg acttacgtag tatatcttta
    13141 cagccttgtc atggtctacg tcattgtctc attggggctg aacattctga caggctatac
    13201 aggacagatt tccatcgggc acgctggttt catgggaata gcggcctacg cttcggcgat
    13261 attcaccggc aggttgggca tcccgtttgt tcccgcaatg atgctttcgg ggctactggc
    13321 ggcggttata gggctgggcc ttggggtgcc tgcgctgcgg cttcatgggc actacctcgc
    13381 catatgcacc atgggctttg gagtcgccgt aaaccagctg tctgcggtct gggacgggct
    13441 gacaggcgga tatcagggaa tgaaggttcc tgccgcatcg atcttcggct acaggttcga
    13501 gagcgacctc agctactact acttggcctt ggcagtgact tggctgatgg ttgttgcagc
    13561 ccggaatatc ctccgttcgc gcccgggtag agccctgatg gccgtgcgcg acagcgaagt
    13621 cgcggcagag gccatgggga taaaccttgc caaatacaaa gtggccgcct ttgccataag
    13681 cgcgttctat gcagggattg ccgggagcct ctatgcgcac cttgtgaagt acgtgagccc
    13741 gtacgatttc aacatggggg tttccatgac attgctcgct tcgatctgcg ttgggggcct
    13801 gggcagtgtg ccagggtcta tcctaggggc gatctttatg acgctgatgc cccatgcttt
    13861 cagccgagtg aaaaacctgc cgttggtcct atcaggaatc tccctgatac tggtagtgct
    13921 cttcctgccc tatgggctgg cgagcttgcc ttggaagata aagagcgcaa gaagtgcaag
    13981 atccacaggg ggtacagcgg ggagggtaga gacgagtggc gatgcttgaa cttgaaggga
    14041 taaccgtgag gttcggggga ctggttgcag tcgactccct cagttttggg gtgcgcgagg
    14101 ggactatcca cggactgata ggtcccaacg gagccggaaa gagcaccgtc ttcaactgca
    14161 tatctgggtt tgtgaggcag gacgaaggga gcatatcctt ccgcgggcag cggcttgcat
    14221 gcgagcccca tgacgtcatc gcacggggta tagcccgaac attccaaaac gtcgagcttt
    14281 tccgcggaca gaccgtactc gacaatgtcc tgatcgggct acacacagcg ggaaccgcta
    14341 atttcatcac cggggcattc cagacccggt cggcgaggaa ggatgaagag cgcctccggg
    14401 agcaagcaat gaaaaccctc gattttttgg gaatcggcga atacgccggc aggatcgcct
    14461 acggtctccc ctatggagtc caaaagctgg tggagctcgc cagggcattg gcgctcaggc
    14521 cgaatctgct cttgctcgac gagcctgttg caggcatgaa cccaaccgag cgaggggagc
    14581 tggcagagat cataaggaac atcaaaaaag agttcggcat gacggtgctt ttggtcgaac
    14641 acgacatgtc tttcgttatg gggctctgcg acgagataac tgtgatgaat tttggccgga
    14701 agatagcgga gggcactccc agcgaggtcc aacaggaccc gcaggtcatc caggcctatc
    14761 tcggggagga tgcaggagat gctgaggcta tctgacgtcg atgtttacta cggcatggtc
    14821 ggagcgctcc gaaatgtgtc gatggaggtg cgccagggtg agatcgtggc ccttttaggg
    14881 gcgaatggtg ccggaaagac cacaacgctg cgggcgatct ccgggatcgt caggcctgct
    14941 tccgggtcaa tcgaatttga gggcagagca atccacggac tttctccgga gcgcatagtc
    15001 tcccttggaa tcgttcaggc ccccgagggg cggcaggtct ttccggagct ttcggtaaag
    15061 gaaaatcttc tcattgggac atattcccgc cgggaccgcg ctaagattcc agaggacttc
    15121 gctcacgttt acgatcattt tcccgtcctg aaggagaggg aatctcagcc ggcagggacc
    15181 ctcagcggag gggagcagca gatgcttgcg atagggcgag ccttgatggc aagtcccaag
    15241 gtcttgcttc ttgacgagcc ttcgctggga ttggccccac tggcaacgaa gcaaattttc
    15301 aagataatcg aggcactcaa tgccgaaggc acaacgatac tcctggtgga gcagaacgcc
    15361 aggcttgccc tagcggtatc gaacagagca tatatactgc agacgggcag cgttgccctt
    15421 tcggggccca gtgatgagct gctcgcaagc gaggacgtga agcgctccta cttgggggga
    15481 agcactggag gcagagacat tgccgaggtt gagcgagctt gatgtatcgc gaagtttcag
    15541 cgtgtagccg aagtcggccc gggcttggcg atttccagga ctgagtaggc gagtcgcggt
    15601 tccgactcgc tggcgaagac aaccgagatc aggatactcg ggattgagcg gtttgagact
    15661 gtatcaggct gcaccttgcc gacaagagcg aagatatgcg cggtgcggta tggtgctgac
    15721 catacgtcaa aaagctgagg aggttagcag gatgaaaagg cattggacgt tggtgctcgc
    15781 atcccttatg gttgttcttc ttatgggaat cccgggaccg gtcacggccg ccccggagcc
    15841 cggcgtgacc gcaaatcagg ttctcatagg cgcgttccag gatcaatcgg gcccggccgc
    15901 tgtggtcgga ataaacatgc gcaagggcat ggaagcctac ttcaactgga tcaactcgaa
    15961 gggcggagtg aatggacgga agatccggtt catagttgaa gacgacggtt ttcagccgtc
    16021 taggtcgatt gcggccgcca agaaactgat tgagcaggac aaggttttcg cgctcgtcgg
    16081 aacgctgggg actcctggcg tgcaggcgac catagactac atccaatcga aaaaggttcc
    16141 gtcaatatac caaggcagcg gtatcagcga attagcgttc ccaccgaaaa ggtatttctt
    16201 cccggttcag cccaacttca tcagcgaggg ccgcattatc gcccaatacg tggtagaagg
    16261 gctgaagtgc aagcggatcg cagtggcatg tgaacagact gacattgggt cagaaggcct
    16321 taggggcatc aaggagcagt tggccgtcta caagatgcaa ccggtcgaag tcgtcaattt
    16381 cggcgcagca gatgtcgact tcagctccca ggttctcaag ttgatcaagg ccaagccgga
    16441 cgcggtgata atctattcca ccctcaaacc gtgcgcaggc ttcctgaaac aggctgatct
    16501 catggggctt gatgcgcagt tcctcacgac atacgtgaat gccgatccaa ttcagatgcc
    16561 ggcgctcgcc ggtgaggcat gtgtggggct gatcgccccg ggttgggtcc cggtgcttgg
    16621 gaacgatgag gattccgtca agtacctcga aatctaccag gccacctatc ccaatgaaat
    16681 gccgagtgct ttcgcggcag ccggattcat tgcggctgag gttttttgtg aggggttgag
    16741 gagatgtggg gccaatccca ccagggaggg cctcgtggct gcaatggaga cattcagaga
    16801 ctggaatgga attatggcta agggcataac ctacacccca gaatggcggt caggcaaggc
    16861 ttcgatgtac ttcatgaggt ttgccaagcc caagttcgag gaggactcga tcgaagtcat
    16921 atcaggctgg gttaagctga agatatgacg ttggggcaca gcttgcgtcc ggagggtcga
    16981 aagcaggcgc cgaaaacggc aacaagggct aagcaagtcg tctaaagcgt attggcagca
    17041 aggctcaaac ctcaatcagg gaaaacggga tgattgcgca aagactatct gagaattgct
    17101 aaacagtata gtctgaagat actacgattt tttcgtagta tcttttctta tgctgcgaaa
    17161 caatcgcagt gttgcaaaga aatatcacgg ggccacagta tctgctctga ccggctccca
    17221 atgacccttc ccatcggcct cccgtttctg ctgccttctt atggagtatg ccccatcgat
    17281 ctgggcaggt ggcctttgcg aggctggcga ttctctgatc tcctggaact ggatcctaag
    17341 tggtgggggg tgctcgagtc ctctctgaga tgtgctcaga tgaaaggtat aacgtgtgaa
    17401 atgttgaacc aatatgaagc gtcaggttac tgctgaagta ggatgtcatc cgcgggcgcg
    17461 ggggctggcc tgaacgtccg cagcgggttg gcatgcgcaa cagagcacga cgttgatgcg
    17521 tcttcgcgac aagacaggta gggcaatcgt atacgttcct atttcacaga ggaggtggtt
    17581 ggacatgccc gacaaagcgt caggacagcc agtcgtcctg gaatacgtga agatgcggtt
    17641 gccggagctt tccgctcttg accgcgaatc cacagtcttc ataataagtg tgagcccaat
    17701 cgaggtccac ggaccccatc tgccggtggg cacggacgtt ttcatcgccg aggagctggc
    17761 cgcgagatac atccgtgagc tttttgcacg ccatcccgag cttacattcg taaccctacc
    17821 ctcgctatac ttgggctcag acgcccttcc ggtgaaggga tcgctttcgg tgcctgctcc
    17881 ctatctagag ggggccttgt tttcttacgg caaaggcctg gcggcccagg gttttcgcta
    17941 tcttttcgtg gccgacaacc atggcggtcc tcgccatcag atggcgattg aagcggcggc
    18001 ccgggcactc tggaagaagc acaagtttta cctcatagat ccgttcaaca tagagttccg
    18061 ctacatggtt gagcacgacc cggatttcgt gaagaggacc ggactcgggc ccggcgtatg
    18121 cggagatgat cccgacagcc atgcagggac aaatgagaca tcgctgatgt tggcatgttg
    18181 cgaggagcaa gtcgctggag gataccgcga agttccggcc tctctgcccc cgccgcacac
    18241 cggaggggca tcattcgtag ccggactggc caagatcgta aggttttttg gcggagatgt
    18301 gctctctcgc gacctggaac accttgcgaa cacgttggcc tgggtaggtg atcccgacat
    18361 gctgccatac atgggcgatc cgagcaaggc cacggtcgag gcgggggagg ccatgcttgc
    18421 tgccagagtt gaagttgcga tggagctatt cgagcgcgcc cttgcgagcg ggggtaaaga
    18481 tccggtgcac ctggagccga tgctttgggg cgtgagagcc atgagaaaac tgccagagta
    18541 gcagaatgcg ggcggattat gtatctggca gatccgctcg gccgacatgg aagaccgctc
    18601 tatcttgcga tcatccgtca actgggccgc aacgcgccat gggctgtaac cgcccatccc
    18661 tgcctcggag ggtgcgttct ccgcattgcg gttatcataa aaacttgcgg ccataagttc
    18721 aaatgacagg caagagatgg aatttgacac atgggagaag tagattatgg agatttgttt
    18781 tttggggggc gctgaggaag ttggcgcctc atgcatactt gtcaaaacag aatcggggaa
    18841 gaacgtgctc ctcgatgcag gcgtcagggt gagcgaagag gggacggaaa tgctccccaa
    18901 ccttgagccg ctcaacgacg tagttatcga tgcgataatc ctgtcccacg cccatctaga
    18961 tcacagtggc gcccttccgc tagtggccaa actttcgccc ggggcatcca tattcgccac
    19021 gacggcaacg cgcgatttgg ccacagtctt gctgtacgat tcagtcaagg ttgcagaatt
    19081 cagggaaggg ctgcagctct tcgacgcaga cgatgcccag caggccctag acagggtggt
    19141 cacatacggg ttcgggactt cttttgaggc agtagagggg attcaggcgg tgttcctcca
    19201 agcagggcac atcctcggcg ctgcggcggt actgcttcag acagaagaag ggacgctcct
    19261 ttacactggc gactttacca catttgagca ggagaccgta ggtatgcagg cgttcagtgg
    19321 agctctcaag cgcggggccg atgtggtgat caccgaggcc acctatggat cgaggattca
    19381 cgctccgagg tctcaggaga tgcaaaggct gctcgacact atcggcgaga cgctggacgc
    19441 tggcggaagg gcgcttgtgc ctgcttttgc cgtgggaagg tctcaggaga taatcttggc
    19501 tctcagaaac tacatgcggc gaacgaagag aaaataccct gtgtacgttg atggtctgat
    19561 acgaaacgta aataccgttt tcgctaggaa cccgaattac ctgaagcaaa gatacaagaa
    19621 agaagtcctg cggggccatg agctttttta cacgaatggt atcgaagcag tggggagcaa
    19681 gtcccaaagg gacaagatcc tcgcatcgaa agatccgtgt gtgataattg cgagttcagg
    19741 gatgctcaca ggcggagtgt cgccgatata cgcagagagg attctcgagg atgagaagaa
    19801 tctcttggcg atagtggggt accaggatga agaatcgccg ggaagaagat tgctggactt
    19861 ggctgagctt ccaaagaatg aacggacggt acttttgaac gaaaacgaat accgggttag
    19921 gtgcaaggtg gggaagttcg gtctttcggc ccatgcggac tcgctcggga tcatcgcttc
    19981 agtaaaggct ttaaggcccg acttcgtcct cctcaaccat gggaatgagg agtctcttga
    20041 agcgctctcc caagccctgg cagacgaatt gcctgacacc cgcatagaag tggccggctc
    20101 gggtggggtc tacgagtatg ttccggcgcc gggcggggga aaacgacgat actccttgaa
    20161 gccgcaggtg cgcaggatag cgttgcacaa agaggagccg gtagatccgg aggagctttg
    20221 gcaacacctg ttccagacag ggactctggg aacgacgctc acagttgcgg atttgatgat
    20281 ggtgtggtac ggtccccgag agatctccga cgaagagaaa cgggctttcc gcaaggaagt
    20341 cagcgggtcg ccgcgcttca ggtccgccta caccaacccc aatattgtgt acgtgttgac
    20401 tgaggaagag tatgtggacg ctgttacgcc gaagccgatg gaacagaatg aggcgtgggc
    20461 actgattcag gaaagattgt cgccgtttgg acttcagaag gtgggatttg cttcagatgg
    20521 aagcgtgacg ctgtattttc cgacgccgaa atatgccgag aggtgcacag acgagatcgg
    20581 agaattggag cgggagatac tgcgcagaat cgaagttgcg ccaaactcca atatggaatt
    20641 cctgaagacc aagatcaaga cggacttggc agaggagttc ggcataaggg tcaaccgcga
    20701 tccgtccttc ggcgcacaat cggtcactgt gaaggagcca gcagggttgg cacagccagg
    20761 aatccgggga cgaacgttgg gagaatatgc agaggcgttc gaggaggaga caggctacaa
    20821 attgctgttt agggttgaag gacagagcct tctggatcag gttgccgcac gacgcagtca
    20881 ggacccgtgg acgccggagg gtgtaccgtc acacggcgtg agtgaaccta tggagcagaa
    20941 tatggcgaag ggcatgatcg acgatgcttt cgcgggagcg gtcaatcaac cgaaggtcag
    21001 catttacagg gatgagggac ggatggcgct cgccttcata accccccagg tcggggagag
    21061 atacaaggag atcctggaca agctcgaggc ggcgacagga tggagactgg aaatccagga
    21121 gagcgcgaga gtgaatgagc tggccgacct ggccagggag ctcctggccg ccaacggcct
    21181 aggagggatg aaggttggcg ttcattcggg ctatgccgag gcaaggggcc ctgtggacgt
    21241 tgacgaggcg gttgcccaga ggctgagcaa ggcctactca gagttgacag gttacgagct
    21301 gaggttcaga cggacgtaga ggctgtacca cttcgtggtt gacgggtttt gccggggaag
    21361 cgaaactaca cgggggcatt acgttgaagg ctgtttatgg gagtgcatta ctgacgaaac
    21421 ctcgcccatc ttggactttg acaatggcgt gggcgttggt ggctgccgca ttgtctgggc
    21481 atgctgcagc aggggagata ggagctgagc ttgttctgtc gcctcaggca agagccatag
    21541 tggagacaaa caggccgatt tgtcctgggc cgtctggtga cgacatcgag agagaggcca
    21601 tagagctgtt gtataactac acaggcctgg aagatgagat tgatgtagcc cagatttggc
    21661 gaaatcaagg ccgatcggtc agaaccggcc aatcgcacca atggcatatc tctccacatt
    21721 atgctgcgga agaagttgaa ctgttgttcg aagtattgaa gtacggatat gctgggtacg
    21781 aatacttcgg cggcgatgaa gccttcgaag cggcaaagga cgggatactc cgcgagatag
    21841 gcgaattcgc ggaggagatt cccatgtggc aatacataga catcatcgcc gcaaatctca
    21901 gtttcgttcg ggatggacat ctctcaattg gggagagaag aatctgtgaa taccatcaat
    21961 tcagggcaag tccgacccac agtttttgca tggataatga gggtcaattc tggaatgccg
    22021 atggcgagag aattctatcc atagatggcc agtccccgtc tgcattcatg aaaccgtcta
    22081 tagatgaagc cggcgacata gtctacctgt tggggatggt tctgcctgag ggaaacgggg
    22141 atgtcgcgct caaactcgaa tacgcagatg ggtgcgatga aatggcctgg cttgtccctt
    22201 tttcacccat ggttcttgac gggcctgtct atgagctgtc cgaggttgga ggcgtgccgc
    22261 tcataacgtg cggagacttt cgtgatcttc ccggccaacg gcgcgccatt gctcgattcg
    22321 ccgaggatgc ggagggtttt cgggatgaag acgtgatcat cttggacctt aggtccaatc
    22381 gcgggggaag gtatgcctat ggtagggaat ggtgtcgccg gttgagcggt ggagacgttg
    22441 ggccgagcac ctattccagg cgactcgtta cagatgtggc attggcattg ttatccaaca
    22501 cgttgtccga atcagatgat aacctgtcta gtctcgaaaa ccaggatgat gcgccttgga
    22561 tgaaggacag aagcgaggac cccgagttgc ccgggtggag ctatgcagtc gtcgaacagg
    22621 aggcccgggc aggtaggacg ccatttgttg ttgtgctcct cgattcatac gtggccagtg
    22681 cagccgaagt cttcgtcaga aggttgcgat attgccaaaa tactgtcttt attgggacga
    22741 atacgcgagg tgccgcgctt tgcggcaatg cgggggtgat ggagctttct tattcagggc
    22801 tgaaggtgag cctgcctact atgatctgtt ttgacggcga gatgcagaat atcgatggat
    22861 gtgggtatgt tcctgatttc tgggtgcatc ctgactttgt gatggagagg gctgtgaggt
    22921 tcatcgagaa gcagctagtg tcggtgcaag ccgatggggc cctcgggcag acgagcgaag
    22981 aagaacctgg aggcattatg cggcaataac gattatccgg aatccatatg atttcttcgt
    23041 gaggtgaggt catgagaaat cccctacacc tgcgcgaatc acgtctattc caggctctca
    23101 tggcattctg cgtaatcgcc attgtgacgc ctgttcaacc tgtgttagca tcatccagga
    23161 cgcgattagt cttatcgccg gaggcgaagg ctatcgtgga ggcgaacagg cctgagcttt
    23221 ccgaggcatc tagggaggat ctgttagcgc gtttttcaag catgctgtat gaatacagcg
    23281 atttcgagga tgaggcgtat ccgagcgcac tcaggtggga cacagctgcg ggccgagaga
    23341 gtgtcatgga ggaatggtcg atcagtccgg caagggctgt gcaagaggta gacctcttgt
    23401 tccacgcact gaagtatggt tatgctgcct atcagttctt tggtggcgat gaagtcttct
    23461 gcgcggcgcg ggagaggatg ctggatgaga taggccgaca ttaccatgat attccgttct
    23521 ggcagtattc ccagatcatc atagctaacc taaagttcat ccaggatggc cattttgctt
    23581 ttggaagcag gacattatgc gaatggagca ctttcagagt cagtccgtat cttcggtttt
    23641 cactggatga agacggcttt ttcattaacg atggcggcaa gcgcgttgct tcagtcagtg
    23701 gccaagctcc gtcttctttt cttaaaccgt caatagatga agatggaaac atcgtctacg
    23761 ttcttgggat gctcgtcccg gagcaacagg ttgatttgat gtgtgagata gaatacgcag
    23821 acggcagcag ggagattgca tggctggtgc caatcatctc catggattac ggcggagcgg
    23881 tctacgagct tggagaaata gagggtttcc ccttggtgtc ttgtcgcagc ttcgatcctg
    23941 atcatgatga agggaaggct ctcaacaggt ttgtggagga cgcggagaag ttgcgacaag
    24001 aggatgttat cttgcttgac cttaggtcaa atcacggggg atcgacgatc tacggcatgc
    24061 tttggtgcca aaggttgacg aaagggaacg tgggcccgta cttttttaca gcagagttgg
    24121 cgactgacac ctctatcgct ctgctcctga atgggctttc ggaaaggctt ggggaggagt
    24181 tttcttccga ccaggccgag gctttgtgga gatactggaa gaatgccgag gacccagata
    24241 ggcccggatg gcgtcgcata ggcattgagc agggtgggca tatcagcgat gctccgttca
    24301 tagtagcact tgtggattcc aacgctggga gcgcgacgga ggcttttgtc cgtcgtctgc
    24361 ggagcttgga taatgttgta atcatcggaa ctaacacccg aggcgctttg ctttgcggaa
    24421 atcccggtat gcttgttctg ccctattcag gtttcgaggt gagaataccc acgatgttct
    24481 ggctcgacag cgagatgcac aattacgacg gcgtcgggta ttctccggac ttttgggttc
    24541 atccagacta tgcgttggaa cgggccgtga agttcattcg cagacatctc gttagagaag
    24601 gggccagtgt ctcacttggc ggttgatgga atcgaacgtc taaagaaccg ggtcttcccg
    24661 ttgcctgctg catgcgttgg gggattgctc tttacattgg cattggtctt ctcatccgc
//
LOCUS       DAOSYL010000035        24558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1678_length_24558_cov_7.899400, whole genome shotgun sequence.
ACCESSION   DAOSYL010000035 DAOSYL010000000
VERSION     DAOSYL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24558
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1678_length_24558_cov_7.899400"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLU15_06945"
     CDS             <1..953
                     /locus_tag="PLU15_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO30697.1"
                     /translation="LKALADGNAQEARGLIDKALVLDSTRVDSLLAASLACWMAGDLE
                     AATDAVKAASCYISDDIGELVMVGLAYARGGNDASTAYHYLMRALSLAEGDGELTSVL
                     ADALLEIGFPDESRDAAERALSIQPELAEASLTLGSAYIALDNYEDAIEHLIRATHGI
                     PDNAVPPFLLGVALYETGQWADAARAFGEAAKREPDDHMIDVWRAKALYETGQTDAAI
                     SIINNLIEEAQEDADLAVEIGNFYGLNAEMPSKAIPLLEKAIKLDPTLSEAYFNLIYA
                     YLLTGNETQARKTLMRLSEIDIELAQLAIENLMSITDEIY"
     gene            complement(1159..1656)
                     /locus_tag="PLU15_06950"
     CDS             complement(1159..1656)
                     /locus_tag="PLU15_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30698.1"
                     /translation="MWLLGSNPFRSVLSIKKPWVINLPFQFQRMSDHPSFSRLWKDYL
                     DAVSPDLTRYEEAMLQAARAVSTRLGVPEGQLPQMILVPNPLQAPQIAEAVAVDERVF
                     LIMAEPELESIVHEMLHHLLAPGIMAAKASIDRAYALWKPVRDDMMRMGYESAIRGGA
                     SLKRT"
     gene            complement(1697..1981)
                     /locus_tag="PLU15_06955"
     CDS             complement(1697..1981)
                     /locus_tag="PLU15_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30699.1"
                     /translation="MSLGKDASSGAAEANMLVQPDKRVFMVYVGLTASGYELASLEEG
                     EPMARVAARAADVEWSPDLLAYYRWARVESREVNLYWPRAFLLVAAGLRI"
     gene            complement(2126..2956)
                     /locus_tag="PLU15_06960"
     CDS             complement(2126..2956)
                     /locus_tag="PLU15_06960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO30700.1"
                     /translation="MDVRRDNGCVCRVREFYESNVEMEWVRLARHRTEFALTARAMEE
                     FLQPASTVLDCGSGPGRYSLLLASRGHKVTLLDISEESLRFAKARAEEQGVRFEECVS
                     ASAVDMSMFEDGSFDVVLLMGPLYHLLDPSDRVTCLTEARRVLRSDGLVFATFLVRYA
                     ALRDVAKYDPEWLADYPDDANRILKDGIYTFEGRDNPPFTDFWSVHPTEAAAIMEKAG
                     FETQILLAQEGLVSMIDEKVNLLQGEAWEAWVDLNYRCASDPTIHGAAEHLLYVGRRT
                     "
     gene            complement(3016..3834)
                     /locus_tag="PLU15_06965"
     CDS             complement(3016..3834)
                     /locus_tag="PLU15_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO30701.1"
                     /translation="MNIGVSTLLYENEDIITAVSSIAEAGQKRIELFCEIAGFRPGEV
                     SDETVDTLADLALEHGITYSIHPPCIGLNPASSDSSERARVVEAYIGTIELATRLGIR
                     DMVVHCGSKSDPRVPTEIALGFASQTLQTVGDVAQQAGVVMMLENTCWKETSILESPQ
                     DLIDLADLCPPSTRLLLDVGHAMIWGVNPTECARAWFPRLAQIHAHDNHGECDEHLPL
                     GDGIIDWKELISFLAMERWDGVFMIEVGQQEDSADALERSLDIVRECLAQCANA"
     gene            5197..5685
                     /locus_tag="PLU15_06970"
     CDS             5197..5685
                     /locus_tag="PLU15_06970"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO30702.1"
                     /translation="MTNRKDLLDMPVMTIDEGAEAGRITGLVIDPISVHAVALIVSTN
                     ERFNAPKAVPFPLVQGLGEDAVMIENKSAMAEFSSLPELTTIGKTDSELCGLTVIART
                     GKIIGYVEDFAVDDETGRIGSFLVRLKGQSLSVDIDTKNVITIGSNALIVIADTVDAL
                     DS"
     gene            5729..6772
                     /locus_tag="PLU15_06975"
     CDS             5729..6772
                     /locus_tag="PLU15_06975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO30703.1"
                     /translation="MRMPTHPVARRLAMAAIAVVSIVVQDATAVVGLPMATNKSPECI
                     NPLAHTCHVLPCLDSKARFENSEGARWRPEKKGLSGIVAGSGMLSVRDRTSSNTKTEV
                     ACPLDEESRLSFVSRTSSAARAAPLLSMTVTKPMDGRKRAILTFDDGPSSTYTPEILD
                     ILKDEGVHAVFFVVGSQAESRPHLLSRMAAEGHEIGNHTYSHLDPASATYEELVCEIR
                     RTSEIVEQATGSPTTWLRPPGGVVSPEVISAAESTGHNIVLWNVDTRDWRVAKQADAD
                     RLVAEAVASATASQVSIVLMHDGGGQRNATVSALRSIIQLLRDNGYEICSLAELVGTT
                     DDGKASPSPIWTR"
     gene            complement(6836..8893)
                     /locus_tag="PLU15_06980"
     CDS             complement(6836..8893)
                     /locus_tag="PLU15_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO30704.1"
                     /translation="MAGENHSCHCSDDNVHGHDCHGHGKGNETARVYETVLALALFAV
                     GMLVKAWSSSEAAVLVSDSILLASYIAAGWRVLWGAARNILRGQVFDEFFLMSIASLG
                     AIAIGELPEAVGVMAFFAVGELLQDRAVERSRTSIRALMDVRPDYANLVRDGECIRVS
                     PEEVSTGDLIMVRPGERVPLDGVIIDGETTMDTSALTGESMPRTFGAGDQALAGMINR
                     SAAVTVRTLRPYSDSSAARILELVEEAETRKAHTERFITTFSRYYTPAVVGLAVAIAV
                     LPPLIIPGQVFSEWLHRALVLLVVSCPCALVISIPLSYFAGIGGASRRGVLIKGADCL
                     EALNDVDSVIFDKTGTLTQGSFRVVETTGENGFTGYEVLRLAAHAGANSSHPVSTSIR
                     EAFEGEPGRGRIDVSVISDSRELAGMGVAAEIGGERVIAGNDRLLHHENVVHDVCVTD
                     GTVVNVAKDGILAGRIRLADEIKPGASEAVQRLHEAGVNRIVMLTGDVGDIAERVAGE
                     VGIGEYRANLLPDEKVRAVEMIMAEQVSMAQSGGGWPDASAHRRACGASGRGAGKVAF
                     VGDGINDAPALARADVGIAMGALGSDVAIEAADVVIMDDNPARIADAIETARRTRRIV
                     MENIVFAIIVKLAVLAMGTMGVATMWNAVFADVGVALIAVVNATRAYGNLGHARGSAS
                     GRA"
     gene            complement(8874..9224)
                     /locus_tag="PLU15_06985"
     CDS             complement(8874..9224)
                     /locus_tag="PLU15_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO30705.1"
                     /translation="MDEVEGRIPEVAGISEIFQILGNETRCRILYLLSFEELCTCHMA
                     EMLGITMPAVSHHLRLLRAHRLVKTRPEGKHVYYSLADEHVLTLIQVAQEHYEEEGLL
                     GAESGGCGDGGRES"
     gene            complement(9272..9460)
                     /locus_tag="PLU15_06990"
     CDS             complement(9272..9460)
                     /locus_tag="PLU15_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30706.1"
                     /translation="MSRWSAEAERPYALAKSLLTQDVVLLQQFRARLEGKVMGAIDIV
                     AFGVLTNQPLHHYDILND"
     gene            complement(10291..10743)
                     /locus_tag="PLU15_06995"
     CDS             complement(10291..10743)
                     /locus_tag="PLU15_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOO30707.1"
                     /translation="MDTAVITDKTKEIRELTCIVCPVGCSLQATIEDGNVLDVEGNSC
                     KRGREYAVDELCAPKRTVTSTVRVEGGFLPLVPVRTAAPIPKEKIDQALLEVAKATLE
                     APVRIHQVIIADVAGTGVDVIASRSMTKTVGTYPAFGAKTVLVHDACV"
     gene            complement(10752..12104)
                     /locus_tag="PLU15_07000"
     CDS             complement(10752..12104)
                     /locus_tag="PLU15_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO30708.1"
                     /translation="MSRIECDVAVIGAGPAGLAAAERAKQSGAGRVILLDRDRELGGI
                     LNQCIHNGFGLQLLAQELTGPEYAEHFINKTFDSGVETMLNTMVLDVTRDRQIIAINS
                     DEGLMRIDAEAIILAMGARERNRGAVTIPGTRPAGVFTAGAAQRFINMEGYMPGRRVV
                     ILGSGDIGLIMARRMTWEGAKVEAVLEILPFPSGLTRNIVQCLDDQGIPLYLSHTVIS
                     VKGEERVEGVTVARVDENLKPVPGSEWDIECDTLLLSIGLIPENELSREAGVELDAVT
                     GGPVVTDEMETSVPGIFACGNVVHIHDLVDNVTREAWAAGEGAARLVTGKQPRGATIR
                     TRAGDGIRYVVPHVVHVQNLADRDVRLFMRATHPAENVRVEVVVGDNVLFSKFERVVR
                     PAEMLALTIPAGRISEEAIASASLADAGRRGEEGSKNGPELVVRVTPRDVHKRGGVQA
                     "
     gene            complement(12097..13617)
                     /locus_tag="PLU15_07005"
     CDS             complement(12097..13617)
                     /locus_tag="PLU15_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO30709.1"
                     /translation="MAATKRITADVVVIGAGIVGTAIARELSKYELSVVLVEKEADVA
                     FGTTKANTALVHAGYDADPGTLKAKLNVAGNALYPKITQELGVLYKNTGSLVVALDED
                     QMLHIHELKIRGEKNGVPRLEVIPAERLLELEPHVNPDAVGALWAPTGGITCPWELAI
                     AYTENAVLNGARVLLSSPVIGIDVHEGRVTTVHCPNISIDARFVVNAAGVWADDIERM
                     AGRDDFEVTPRRGEYYLFDKRASRIVARPLFPVPTPFSKGIVVAPTVDGNLLAGPNSV
                     LTGRKDDYATTAPGLAEVLEGARKLIPDLPVREAITNFAGLRAVAEPGGDFIIGVSPG
                     LSNFINAAGIQSPGLTAAPAIAEMVRDILSDAGLELMEKPDFDPIRKRPLRFTELTRE
                     EQARMVAEDPKWGHIICRCETVTEAEIVAALHSPVPCTTVDGVKFRTRAGAGRCQGGF
                     CGPRVVAIIARELGIPVEKVTKKGGKSRIVMGPSKEPLLECSCSVEGMEKEVGDDV"
     gene            complement(13643..15148)
                     /gene="glpK"
                     /locus_tag="PLU15_07010"
     CDS             complement(13643..15148)
                     /gene="glpK"
                     /locus_tag="PLU15_07010"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOO30710.1"
                     /translation="MGEYILALDQGTTSSRAILFDHLGRSVGVASREFKQIYPKPGWV
                     EHDPNEIWRTQLKTARGVIDSTGVGPDEIAAIGITNQRETTILWDKSNGCPIGNAIVW
                     QCRRTAGCCEELKAAGEAKAIHEKTGLIVDAYFSATKIKWILDNAPDLRGRAERGEIL
                     FGTVDSWLIWNLTGGRVHATDYSNASRTMLFNIHTLDWDDEILEILDIPRAMLPEVRP
                     STGVFGYTQPSLLGAEIPIAGVAGDQQAALFGQACFSPGSAKNTYGTGCFMLMNTGED
                     VVMSRNNLLTTIAWGIDGRVEYALEGSVFIGGAVVQWLRDGLRIIDSAAESEECANRV
                     ADTGGVYVVPAFVGLGAPHWDPFARGTIVGITRGTERDHIVRAALEAICYQTRDVLEA
                     MTADSGIELKQLKVDGGAVANNFMMQFQSDILGVPVARPKVIETTALGAACLAGLGVG
                     FWNGQDELACTWQRDREFTPKMCHGEREKLYAGWKRAVERSRNWEQSISPC"
     gene            15827..18958
                     /locus_tag="PLU15_07015"
     CDS             15827..18958
                     /locus_tag="PLU15_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO30711.1"
                     /translation="MSKASSLEKSLAKKLNRDISCYEKDGAIVLEGNVPTWDHVVAAG
                     KSGAKLGYKGVVNRLTCDGILAMPGPKAPSIRDASLEGERMDVLVIGGGVIGTAILRE
                     LSRWDIRACLIEKEADLAMHQSGRNAGMIHPALAPSPGSKKAQFNSLGNPMIPAIADE
                     LGVPFRYNGMTIIVERRIYGIAYPVLANRARRNRAGEIRFVPPAELQSMEPNVRDDIG
                     GAFFMPQSGIIDPFMLTIAFAENAVENGATVWLNTMAESFIVEHGRILAVVTNRGVLF
                     PKAVVNAAGLWADTVAEAAGDMFFTIHPRHGEMLILDKKKGNLARSTLSMPPVYQART
                     DTKGGGVIPTIDGNILIGPDAVELPYREHYATRAERTENLVKKQQRFINGISASDVIT
                     YFAGTRAATYEEDFVIERSERVRNLVHAAGIQSPGLTSSPAIAHEVASMCIDVLKETH
                     EVEVRPNAKFNPRRNAPPDFARLPLEERQRIIKANPDYGRVVCRCEVITEGEIRAALR
                     SPVPPTTLDGLKRRVRTGMGRCQGGFCTPLVLDIACDETGIAPDKFTNKGGASYLLSS
                     PTRPELEEAISVNPRRDAPIGHAEVLSATNDEDCTPQALGAYDVVIIGGGPAGLAAAI
                     AAHDAGAKVGLIEREARLGGILKQCVHDGFGTIEFNTHLTGPEYAERFMSQAAQRDIT
                     CYTQTFVLDVARNGDGRKPDNFRPSEGRNFALTVENRDGIFNIESKALVLATGCRERT
                     ARQVFIHGTKPAGIYSAGTAQYMVNVQGRLPGRRCVILGSGDVGLIMARRLTLEGATV
                     VAVYEAKSEPTGLPRNISQCLDDFGIPLYLSHTVTKVFGDERVEAIEIAQVDGEMKPI
                     VGTEQRIECDCVIVAVGLIPENELAETLGAEISNTTRGPKVDQTFQTTIPGLFACGNC
                     VHVHDLVDYVTQSGRVAGLSAAQYAQGERRIGQITHVCPSDDLLYSVPQFIDAEMANE
                     AFTIYFRSRGTHQNVVVRLTQSGKVLAESKYPVVRSQQMQAIGVKATNASFDPYAPLS
                     LELAMRQSNMGEE"
     gene            18964..19407
                     /locus_tag="PLU15_07020"
     CDS             18964..19407
                     /locus_tag="PLU15_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOO30712.1"
                     /translation="MTAQSVGKGQISQHKAFTCIVCPRSCDLTAELVEDGWRISGNRC
                     PMGRDFALKEVNNPTRVVCSTARTTDRSCPLLPVRTDIEVPLEDVPRVMAEIIKAVVD
                     VSTGVDMGDVIIHNVAGTGANVIASASATMPMGVSSIETRTDRFH"
     gene            19379..20935
                     /locus_tag="PLU15_07025"
     CDS             19379..20935
                     /locus_tag="PLU15_07025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOO30713.1"
                     /translation="MKQGLIVSIDCGTQSARALLFDCRGSLVAKGKAEFEPYFSTQPG
                     WAEQDPEVYFDAICKACHDLASSAPDDWGRIVGVVLTTQRDTCVVVDENGNVLRPAML
                     WLDQRMAECNKPYPFIYRLAFGAVGMLETAELTRKKCKSQWIRENEPDIWDKMDKFLL
                     LSGWLNYKLTGNMADSVANQIGHVPFDYKHKRWPVSKLDYRWYMSGLSRRHLPQLVRP
                     AQVIGTITPEAAEATGLPVGTPVYAGGSDKGCETLGTGCLNPTSACISLGTTATLQIT
                     TEKYMEPIKFMPPYPAVVPGHFNPEVQIYRGYWMINWFKQEFGYREVLIAEQRGVAPE
                     VILNELLHETPPGCMGLMLQPYWGAGLRMPEARGAVIGFGDIHTRAHLYRSIIEGVAY
                     ALRDAADKVEKVSGTKIERIMISGGGAQTDSICQITADVFNKPVLRGETYEGSGLGAA
                     IIGFCGAGYYSSFDDAVANMVHYEARFQPDPPTARTYDQLYKCVYKKMYSRLKPLYEE
                     MRAITGYPES"
     gene            20952..22673
                     /locus_tag="PLU15_07030"
     CDS             20952..22673
                     /locus_tag="PLU15_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO30714.1"
                     /translation="MSNKKPFEPDWYEGPIPPKSYRSIFKWGGADEYKHPNLHLYELM
                     KEVFEMTDEDFRAPVNLGLEEVSFDVPITLSGEHLKAITNIVGTENVATDDYSRLKAS
                     YGKMIYDNIRLREGIVENLPDIVVYPRHKQDVSEIIGYCNRHKIAVYPRGGGSGVMRA
                     TECVHGGISLDLTKHMNRILTLNEVNHTITVEPGIYLPDLEARLNKAPEFFGASRRYT
                     AGYLPQSFIGATVGGSVVTRGAGQNSTYYGKIEDLVLCQEYVTPAGDIKTKEFPRAAV
                     GPSIDQIMIGSEGTYGVLVSVTLKIFRYTPETTQRFSYIFPTWSNGLAASREMMQSRF
                     GYPSVLRISDPEETDVALKLYGVEDTILDKMMDARGYKRMERCLMLGMSEGEENFAKN
                     VKRQINRIAKRYGAMNTTGYVAKSWEKGRFTDPFMREDLHDYGIIIDTLECAVAWDKI
                     ESLWAETRKVCKARPKTVVMSHSSHFYPQGTNLYFIFIGRFETIDEYLDYQASIIDSI
                     RANGGALSHHHGIGKMLAPWYQDAMGENHVAVLRALKRHFDPNNIMNPGGTLALDLEE
                     SFRRPIE"
     gene            22723..23445
                     /locus_tag="PLU15_07035"
     CDS             22723..23445
                     /locus_tag="PLU15_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO30715.1"
                     /translation="MIDKLSPVPFYIQLRDGIKRNVIEGVWKVGEQIPTENELMEKYE
                     VSRATVRNAISSLVNEGFLVKQRGRGTFVARQQPSMAFEPLISFAYNVESMGASSESQ
                     VVENSIIVPDEELLRLLRWKEPKECLYVKRVRHGDGIPLAIEESFFNDSTAKFLKLKD
                     LSGSISRLLLEELRISISRVEQIVAARKATAEEKKLLNLDKEPQVLEMERWIILEDEE
                     EPFYYLRFIMRGDLYSLTPVPR"
     gene            complement(23605..24204)
                     /locus_tag="PLU15_07040"
     CDS             complement(23605..24204)
                     /locus_tag="PLU15_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO30716.1"
                     /translation="MHNDYMVPVADKRQLILDAAQHVFAEKGFHTSTMDAVAEAAGVA
                     KGTIYLYFKGKNELLIELVDDRTRRLTDMILNGIASAEDVFGKVFAVIEAHFQFYSIH
                     SEFMLLFSGQLGLLSPEMEERLVTGTAALINMVCGVIEEGMALGVISSDVDPMRASFA
                     LEGMIHSVAYQWLSAGKSVSVSVVSHEVYTLFTHGIACP"
BASE COUNT         6017 a         7173 c         6099 g         5269 t
ORIGIN      
        1 ggctgaaggc cctggcggac ggaaacgctc aagaggcccg agggctcatc gacaaggcac
       61 tcgtacttga ctcaacacga gttgattctc tgcttgcagc atcattggcc tgctggatgg
      121 ctggagacct cgaggcggcc acagatgcgg tcaaggcagc ctcgtgctac atatcggatg
      181 acatcggaga actcgttatg gtcggtctgg cttatgcacg tggaggaaat gacgcttcga
      241 cggcctacca ttacctgatg cgggcgctga gccttgctga gggcgacgga gaactcacat
      301 cagttctcgc agacgccctt ctcgaaatag ggttccccga tgaatctcgg gacgctgccg
      361 aaagggctct gtcaatacag ccagaacttg cagaggcatc actgacgctt ggcagtgcct
      421 atatcgccct cgacaactac gaggatgcca tagagcacct aatcagagca acccatggaa
      481 taccagataa cgctgttccg ccatttcttt taggagtagc tctatacgaa acgggccaat
      541 gggccgatgc cgccagagcc tttggcgaag ctgcgaagcg tgaacccgac gatcacatga
      601 tcgacgtctg gagagccaag gccctctacg aaaccgggca aacggacgcc gcaatcagca
      661 taatcaacaa tctcatagag gaagcccaag aagatgccga ccttgcagtc gaaataggca
      721 atttttacgg gctgaacgca gagatgccaa gcaaagcaat accccttttg gagaaagcta
      781 tcaagctcga tccgacgctc tccgaagcct atttcaacct gatatacgcc tatctgctca
      841 ctggcaacga gacacaggct cgcaagaccc taatgaggct ctctgagata gatatcgaac
      901 tggcgcaact agctatcgaa aacctcatgt cgataacaga cgagatctat taggtttcga
      961 gccaactgat gccgcagaca gctggacgcg gctctatggc cataggtcct ttttaggtag
     1021 aggaatcgct taggcgagct ggtgcgccag ctcgctttgc ctcagcttcc tgtgactgtg
     1081 ggcaccctcc aacctctccc gttaccagat ccagatctgc ctctgcatgc tgcactaccc
     1141 agatcgtccc tgccctcact aagttctctt caaagacgcg cctccacgaa tcgcactctc
     1201 gtatcccatt ctcatcatgt cgtctcgcac aggcttccat agagcatatg cccggtctat
     1261 gctagccttg gcagccataa tcccaggagc caaaaggtga tggagcatct cgtggactat
     1321 ggattcaagt tccggctcag ccataatcaa gaagaccctt tcatccacgg ccacagcctc
     1381 ggcgatctgc ggcgcctgta gcggattggg cacgaggatc atctgaggca actgcccttc
     1441 aggaacgcca agtcgagttg agacggcacg cgccgcctga agcatggctt cttcgtatct
     1501 tgtcaagtcg ggagatacag cgtctaaata gtccttccac agtctgctga aggacggatg
     1561 atcggacatg cgctgaaact ggaagggaag attgatcacc cacggcttct ttatgctcag
     1621 gaccgatcgg aagggatttg atccaagaag ccacattgct gccgcccggg cagccccaat
     1681 cccagtcccg gccctcttag attcgcaagc cggccgcaac caggaggaac gctcgaggcc
     1741 aatataggtt gacttcacgt gattcaaccc tcgcccacct atagtaggcg agcagatccg
     1801 gcgaccactc aacgtctgca gctcgcgcgg caaccctcgc catgggttca ccttcttcga
     1861 gcgaagccag ttcgtatccg gacgccgtca gcccaacata gaccatgaaa accctcttgt
     1921 caggttgcac caacatgttg gcctctgccg caccagatga tgcgtctttg cctaagctca
     1981 ccgaaagccg cccccaaacc gtctcatcaa tgcgccaaca agagatcaac tgtacgccat
     2041 atgtcggcct ggacaatccg accttttctg cctgaggttc tagccttggc tgccgggaaa
     2101 tgctcgagtg ctttcccgag gctgcctagg tgcgcctacc aacataaagc aggtgttctg
     2161 ccgcaccgtg aattgtgggg tctgaagcac aacggtagtt caagtcaacc catgcctccc
     2221 aggcttctcc ttgcaataga ttgaccttct catcaatcat gctcacgagt ccttcctgcg
     2281 ccaagagaat ctgagtttca aagccggctt tctccatgat agctgctgcc tcggttgggt
     2341 gcacagacca aaaatcagta aacggcgggt tgtcccgccc ttcgaaggta tagatcccgt
     2401 ctttcaaaat gcgatttgca tcatccgggt aatcggccag ccactccgga tcgtacttgg
     2461 ccacatcccg cagagcggcg taacgcacca gaaacgtggc aaagaccaga ccatcgctgc
     2521 gcagaacacg tcttgcctcc gtaagacagg taaccctatc agatggatcc aaaagatgat
     2581 aaaggggccc catcagaagt acaacatcga aactgccgtc ttcgaacatg ctcatatcca
     2641 cggcggaagc acttacacat tcctcgaacc ggacaccttg ctcttccgct cttgctttcg
     2701 cgaacctgag gctctcctcg gagatatcca gcagcgtaac cttgtgtccg cgagaggcaa
     2761 gcaagagaga gtaccggccg ggaccgcttc cgcagtccag gacggtagat gccggctgca
     2821 agaattcctc catggcccgc gcagtcagcg caaattccgt cctatgtctt gcaagccgca
     2881 cccattccat ctcgacgttg gattcgtaaa actccctcac cctgcatacg cagccattat
     2941 cgcgtctcac atccacgcag tttccctccc caccttttgt gccagcagag gctgcaggcc
     3001 gactctatca cctactcacg cattggcaca ctgggccaag cattcgcgca ctatatcgag
     3061 agaacgctcg agcgcatcag cagagtcctc ctgctgtccc acttctatca tgaatacccc
     3121 atcccatctc tccattgcaa ggaaggatat gagttctttc cagtctatga tgccatctcc
     3181 cagcggcagg tgttcatcac actcgccatg gttgtcatgg gcatggatct gcgctaacct
     3241 aggaaaccaa gcgcgggcac attcagtagg attcactccc cagatcatcg catgccccac
     3301 atcaagaagc agccgcgtgg aaggcggaca caagtcagcc agatcgatga gatcttgggg
     3361 gctctccaga atgctagtct ctttccaaca cgtattttcg agcatcatca ctaccccggc
     3421 ctgttgtgca acgtccccca cggtctgcaa tgtctgactc gcaaagccca gggcgatctc
     3481 cgtcggcacc ctagggtcgg acttgctccc acagtggacc accatatcgc gaatgccgag
     3541 cctggtagcg agttcaatag taccgatata ggcttcaact acccttgccc gttcggacga
     3601 atcagatgag gcagggttca gacctataca aggcggatga atggagtacg taattccatg
     3661 ttctaaagcg aggtccgcaa gagtgtccac ggtttcatcg gaaacctctc ccgggcggaa
     3721 accggcgatc tcgcagaata gttcgatcct cttctgaccc gcctctgcga tactggaaac
     3781 agcagtgatt atgtcttcat tttcgtaaag gagagtcgat acaccgatat tcaagacttg
     3841 taatccccca tccctgtgtc taatctatga acactgtttc tatgtgaaga atgcaaaacc
     3901 tctcagcccg agttgagcat cacaggaaaa cccgtctttc acgtcgggca cagcaggcaa
     3961 cggcatagct ccgtgaatgt cacagggcgc cacaaaatgc taactcccta tttcgcaatg
     4021 ttcctgtgct tttcgacagt tcgatttcaa tccagtaccg ccgccacagc ctatcacatc
     4081 tttgactccg ttcaatctaa atcgcgacat gctgcggtcg aaggccccat actgtcactc
     4141 aagtatgacc atggcaagca cgactcgcaa tcggcgcgat agagcatata cccgaagcct
     4201 ctcgataact gcagacaaaa actgtagatt ggaataaatc aatggaactt tcttcttgac
     4261 tcaagctgtc tcaaaacata tatttttaga taccacatca cttgaaccac atgatatcta
     4321 gcgcttttcc tctcaatgct caatgccaac aggggggata taaccttgac ctgttcagct
     4381 attacctcaa taatggcatc tcttctctct tcggtttcta tgatgtctac aacctacagg
     4441 tctctgtggc agctaccctc ctttaacaga agtatagcga tccttgcctt gggagccttc
     4501 gccgtggcat gcgacgcggt agtccgcacg tttttttcga catcgtccat agacagaagg
     4561 ccttgcttgg ctacagccat agtcccgtta ttctcgtcct tctctcctcc agatgccaaa
     4621 ggtccccagc caagacatta tgccctttcg cggacccgaa ctggatcgag tgaaggcaac
     4681 gcatgcgagc caacaggccg accctggttg ccgaagctct ttttattcac catttccctc
     4741 acgtcattaa tgcgcgaact attagataga tctctccgag tcgtgaagca acttcttcca
     4801 tggatagcga ggtccggtac aggagagcaa ttgcacgtca agcggggcgc gtcacagcgc
     4861 gacgatatcg gatgcattgc caatatagcg ctggattacg gcatcgactt ggcgttcgac
     4921 tggtcatctc ctctcatcct cactccgttt gtggaatgga ccgagccatc gatagacaac
     4981 gccttaagct ccgtcatgcg cctgttaacc ctttctgaag ttgcaagaga tgaggcaatc
     5041 agctgtaagt tagtggagat acaaagatct cttcatgcag ctaaatcttc gttgagatta
     5101 cgcgaccgcc ttagtccgca ggaaaaaggc ctgtttgacc aacgactgcg tgaacgagta
     5161 agtagactca atgtgatgct gagggggtag tcggttatga cgaaccgaaa agacttgctt
     5221 gatatgccag tgatgaccat agatgagggg gcagaggcag ggcgcataac cgggctggtg
     5281 atcgatccca tcagtgtgca cgctgttgcg ctcatcgtct ctaccaacga gcgtttcaac
     5341 gccccgaaag cagttccgtt tccattggta caaggtctag gtgaagacgc agtgatgatc
     5401 gagaacaaat cggcaatggc cgagttctcc tcgctgccgg aactgactac cattggaaaa
     5461 acagattcgg aattgtgtgg actcacagta atagcccgca ccgggaagat aataggatat
     5521 gttgaggact ttgccgtgga tgatgagaca ggcagaatcg gatcattctt ggtgcggctg
     5581 aaagggcaat cactctcagt tgatattgac acaaaaaacg tcatcaccat aggctcgaat
     5641 gcccttattg tgatagctga cactgtcgac gctctagatt cataacacgc gcacagtcca
     5701 tcaggtctca gacttggagg tctgtgaaat gcgcatgccc acgcaccctg tcgctcgcag
     5761 gctggcgatg gcggcaatcg ctgttgtcag catagtggtg caagacgcaa ctgctgtggt
     5821 aggactcccc atggcaacaa ataagtctcc agagtgcatc aaccctttgg cccacacatg
     5881 ccatgtactg ccttgcttgg attcaaaggc ccgtttcgag aattcagaag gcgcgagatg
     5941 gcggccggaa aaaaaggggc tctcagggat cgtagccgga agcggaatgc tgtctgttag
     6001 ggatcgcaca tcaagcaaca ccaaaactga agtcgcctgc ccgctcgacg aagaatcaag
     6061 gctttccttt gtttccagga catctagtgc tgcacgtgcg gcgccgttgc tgagcatgac
     6121 cgtgaccaag ccgatggacg gtcggaagcg cgcgatcctc acattcgatg acgggccctc
     6181 ttcaacctac acccctgaga tcctcgatat tctgaaggac gaaggtgtcc acgcagtctt
     6241 tttcgtcgtt ggcagtcagg cagaatcccg tccccatctg ctctcaagga tggccgctga
     6301 aggacatgag atcggcaatc acacttacag ccatctcgat cccgcatctg ctacatatga
     6361 agaacttgtc tgcgaaatac ggcgcacttc tgaaatagtc gagcaggcca cgggctcacc
     6421 aacaacttgg ttgaggccac ctggcggagt cgtatcaccg gaagtgattt ccgctgcaga
     6481 atcgaccgga cacaatatag tcctgtggaa tgtggatacc cgggactggc gcgtcgccaa
     6541 acaagcagat gcagatcggc tggtggcgga agctgttgcc agcgcgacgg cttcgcaggt
     6601 gagcatagtg ctcatgcacg atggcggagg ccagagaaac gccacagtct ccgcgctaag
     6661 gtcaatcatt cagcttttga gggataacgg ctacgagata tgctccctcg cggagctagt
     6721 cggcacgact gacgatggta aagcctcacc gtcgccaata tggacaagat gaaaccagag
     6781 agcgggcatg tgcgtctccc ctgccagtct gccagcccac ggcgccattg gcgcctcagg
     6841 cccggccgct cgcagatccc cgcgcatgcc ccagattccc atatgctcta gtcgcattga
     6901 cgactgcgat taacgcaaca cctacatcgg caaagacggc gttccacatg gttgccacac
     6961 ccatggtgcc cattgcaaga accgccagct ttacaattat ggcgaaaacg atgttctcca
     7021 tcactatccg cctggtgcgc cgcgcagttt cgatggcatc tgcaattcgg gcaggattgt
     7081 cgtccatgat caccacgtca gctgcctcaa ttgccacgtc agatcccaac gcccccattg
     7141 caatgcccac gtcggctctg gcaagggctg gagcatcgtt tatcccatcg cccacaaaag
     7201 caacctttcc ggctcccctt cccgaagcgc cgcaagccct tctgtgtgca gacgcatctg
     7261 gccagcctcc ccccgattgt gccatgctca cctgctcagc catgatcatc tctaccgccc
     7321 tgaccttctc atcaggcaga aggttcgctc gatattcgcc tatcccaact tcgccggcca
     7381 cgcgctccgc gatatcgcca acgtcgccag tgagcatgac tatcctgttg acgcctgctt
     7441 catgaaggcg ctgcactgcc tcactcgccc ctggctttat ctcatctgca agacggattc
     7501 tgcctgcgag gatcccgtcc ttggccacgt tcaccacagt cccgtcagta acgcatacat
     7561 catggaccac gttttcatga tgcaacaggc ggtcgttccc ggcaatcacc cgctcccctc
     7621 cgatctcagc tgcaactccc attccggcca gttcgcggct gtcggagatt actgagacat
     7681 ctatcctgcc ccgaccgggc tcaccttcga atgcttcacg gatggatgtc gataccgggt
     7741 ggcttgaatt ggcgccggcg tgcgccgcca gcctgagcac ctcgtaccct gtaaaaccgt
     7801 tttcgcctgt tgtctccaca acccgaaatg agccttgagt gagtgtgcca gtcttgtcaa
     7861 agatcactga gtccacatcg tttagggcct ccaaacagtc tgcgcccttt atcaacacgc
     7921 cgcgcctcga cgctccccct atgcctgcaa aataactgag ggggatcgag atcacgagtg
     7981 cacacgggca tgataccaca agaagaacca gagccctgtg tagccattcg gagaaaacct
     8041 gaccgggtat gatcagcgga ggcaagaccg caatcgcaac ggccagccca acaactgccg
     8101 gagtatagta tctgctgaat gttgttatga accgctctgt gtgggccttc cgagtttcgg
     8161 cctcttccac cagctcaaga atgcgagccg ccgacgaatc cgaatagggc ctcaaggttc
     8221 tgacggttac agcagcgctt ctgttgatca tccccgccaa tgcctgatcc ccggcgccga
     8281 aggtccgagg catcgattcg ccggtcagag cagatgtatc cattgtagtc tcgccgtcga
     8341 taatcacgcc gtccaatgga accctctcgc caggcctaac catgatcaga tcgcccgtag
     8401 acacttcctc cgggctgacc cgtatgcact ccccatcacg cacaagattg gcataatcag
     8461 gccttacatc catcagcgcc cttatggaag tgcgtgaacg ctccacagcc ctgtcctgca
     8521 agagctcgcc aacggcgaag aaagccatca ctccaacggc ctcgggcaat tctcctatcg
     8581 caatagcacc gagagatgcg atgctcatta agaagaactc gtcgaacacc tgcccacgga
     8641 gtatgttgcg ggctgcgccc cacagtaccc tccagcccgc cgcgatatac gaggccagaa
     8701 gtatgctatc ggagactaga acggcagcct cagatgaact ccaagctttt accaacatgc
     8761 caactgcgaa aagagcgagc gctaacaccg tctcataaac ccttgcagtt tcgttaccct
     8821 ttccatgccc atggcaatcg tgtccatgaa cattgtcatc gctgcaatga caactatgat
     8881 tctctcccgc catctccgca tcctccactt tctgccccaa gcaagccttc ctcttcgtaa
     8941 tgctcctggg ccacctgtat cagtgtcaaa acatgctcat cagcaagaga ataatataca
     9001 tgcttgcctt ctggccgcgt cttcacaagg cgatgggccc ttagaagtct cagatgatga
     9061 gatactgcag gcatggtaat gccgagcatc tcagccatat gacaagtgca aagctcctcg
     9121 aatgaaagca aatagagaat tcggcatctc gtttcattac caagtatttg gaatatttct
     9181 gagatgccgg ctacttctgg gattcttcct tcaacttcgt ccatacagac acccccagag
     9241 ccatctggcc attcaacgat taagcaatcg tttaatcatt gaggatatca taatggtgta
     9301 atggctggtt tgtcaatact ccaaaagcta ctatatcgat tgctcccatg actttaccct
     9361 caaggcgggc acggaactgc tgtaaaagaa cgacgtcctg agtaagcagt gacttcgcca
     9421 gtgcataagg ccgttcggct tcagcagacc acctactcac agtggcaaga gcctcatccc
     9481 gtctgagcct tggagctcgg gaatctgtct cccgcataaa gacctggcaa catttcttct
     9541 gccggcaagc tcaagagaac ggaaatgatc aggggttaac tcgaccgaga agagcatttg
     9601 tcagccatat ccctgaaaaa cgcatttctc cttcaacact caacaacacc cccagcccat
     9661 ctgccgtgca cagataaagg agagtgacag cggcttctat cctcacaggt agttcgggaa
     9721 cccagcaggc ccgtgtactc acaaaagaaa cccgatttcc gtcggtcagg agatcgtcag
     9781 ggaatgcgtg gacacaaaga tgctgctagt cttgtcaccg aggaaagcta tggaaatggg
     9841 aagaaccagc gcatcctggt tggggcagcc tgataggaaa tggaggggat acatatcaat
     9901 actaggtctc gtgggaagct gtgccccacg tatgagcaat cagttccgca tcgtaggcgc
     9961 cgctcatctg ccggaacaag gcaatcgcga tcagctttaa gaaattggca gcctcacgtg
    10021 taggacagcc ggcctcttca agctccgcta cgaatatatc tcgcccccgc tcgctagtgc
    10081 atcccaaggc gccacgacag gcataacccc tacagcaatg acaggaacga caaaaactgc
    10141 gagggcctta cgcgaccaca tggctcgttt tggtagactg aagagcagcc gttgcctact
    10201 atatcccgca tactacgcaa atagcctcat gaaaaaggca tatattcagg catgaaatca
    10261 ggcgcggcac aggaccgcgc caatgccaag ttacacgcat gcatcatgaa ccagaaccgt
    10321 cttggcacca aaagccgggt atgtgccaac agttttcgtc atggatctgc tggcaatcac
    10381 gtcaacgccg gtgcctgcca cgtctgcaat gataacctga tggattctta caggcgcctc
    10441 caaggtagcc tttgcgacct ctaacagagc ctgatcgatc ttttccttgg gaatcggcgc
    10501 agcagtgcgc acaggcacga gcggaagaaa cccgccttcc accctcaccg tggaagtaac
    10561 agtccttttc ggggcgcaca gctcatccac tgcgtactca cgcccgcgct tacaggaatt
    10621 gccctcaacg tctaggacat tgccatcctc aatagtggct tgcagactgc aaccgacagg
    10681 acatacaatg catgtaagct ccctgatttc ctttgtcttg tcagtaatga cggccgtgtc
    10741 catctctgca ctcatgcctg tactcctcct ctcttatgaa cgtcacgcgg tgttacgcgc
    10801 accacaagtt caggaccgtt tttcgagcct tcttctcccc gccggcccgc atcggccaag
    10861 ctcgcactgg ctattgcctc ctcagagatt cttcccgctg gtatagtaag ggccagcatc
    10921 tccgcaggac gcacgacacg ctcgaacttg ctgaacaaca cattgtcgcc gaccacaacc
    10981 tccaccctca cgttctccgc aggatgcgtt gcccgcatga agagacgcac gtcgcgatca
    11041 gcgaggtttt gcacatggac tacgtgtgga accacatacc ttatcccgtc tccggcgcga
    11101 gtgcgaattg tggcacctcg cggctgcttt ccggtcacga gccttgccgc accctccccg
    11161 gctgcccagg cctccctggt aacattatcg accaggtcat ggatgtgcac tacattgcca
    11221 catgcaaaga tgcccggaac tgatgtctcc atctcatctg ttacaacagg tcctccagtg
    11281 accgcatcca gctctacgcc ggcctcacgg ctcagttcgt tttcgggtat cagcccgatc
    11341 gaaagcagaa gcgtgtcgca ttcgatgtcc cattcggagc cgggcaccgg cttgaggttt
    11401 tcgtccaccc tggccacggt cacaccttcg accctctcct ctcctttcac ggagataaca
    11461 gtgtggctca ggtaaagcgg aatcccctgg tcatccaggc attggactat gtttctcgtc
    11521 aagcctgaag gaaaaggcag tatctcgaga accgcctcga ctttggcgcc ttcccacgtc
    11581 atacgcctcg ccataatcag gcctatatct cctgacccta gaatcacgac gcgcctgcca
    11641 ggcatgtatc cctccatgtt gataaatcgc tgcgccgccc cggcagtgaa aactcccgcg
    11701 ggccgcgttc cggggatagt tactgcccca cggttgcgct ccctagcgcc catagccagt
    11761 atgattgcct ccgcatctat cctcatcagc ccttcgtcgc tgttgatcgc gattatctgt
    11821 ctgtcgcgtg ttacatccaa gaccatggta ttgagcatcg tctctactcc ggaatcgaat
    11881 gtcttgttga taaaatgctc cgcatactcc ggccctgtaa gctcctgcgc cagcaactgg
    11941 agcccaaatc cattgtgtat gcactgattc agtattccgc ccaactcgcg atcacggtcc
    12001 aacagaatca cacggccggc gcccgactgt ttcgcccgct ccgcagccgc cagaccagcc
    12061 ggtcctgcac ctatcaccgc cacatcgcat tctatcctag acatcgtcgc cgacctcctt
    12121 ctccatgccc tctacgctgc acgagcattc aaggagcggc tccttgctag gtcccatcac
    12181 gatacgggat ttgccgccct ttttagttac tttctccaca ggaatgccta gctcgcgtgc
    12241 gatgatcgca actaccctcg gcccgcaaaa gcccccctgg caccttcctg caccagcgcg
    12301 agtgcggaac ttcaccccgt caactgtggt gcagggaacc ggcgagtgca aagccgccac
    12361 tatctcggcc tcggtcacag tctcacacct gcatatgatg tggccccatt tcggatcctc
    12421 cgctaccatc cgagcctgtt cttctcgggt caattcggta aatcggagcg gacgcttgcg
    12481 aatgggatcg aaatcgggct tctccatcaa ctccaagccg gcgtctgaca gtatatcgcg
    12541 gaccatctcc gctatggcag gcgccgccgt aagtccggga gactgtatgc ctgctgcatt
    12601 tatgaagttt gacaaaccgg gcgacactcc tatgatgaag tctccacccg gttcggctac
    12661 tgcgcgaagt ccggcaaaat tggtaatggc ctccctcact ggaaggtcag gaatgagctt
    12721 cctagcacct tccaaaacct cagccaggcc cggagctgtt gtggcataat catctttcct
    12781 gcctgtcaaa actgaattgg gtccggcaag caggttcccg tcgactgtag gggcaaccac
    12841 gatccctttg gagaacggcg taggcactgg gaaaagcggc cgtgcaacga tacgagaagc
    12901 cctcttgtca aagaggtagt attctccccg tctgggtgtg acttcgaagt catcccggcc
    12961 tgccatcctc tctatgtcat cagcccaaac tccagcggcg ttcacgacga acctcgcatc
    13021 tatagagata ttggggcaat ggactgtcgt aacgcgcccc tcgtgcacat ctatgccaat
    13081 caccggcgaa cttagcaata cccgtgcccc attgaggacc gcgttttctg tataggcaat
    13141 ggccaactcc cacgggcagg ttataccgcc tgtgggagcc cagagcgcgc caaccgcatc
    13201 cggattcaca tgcggctcaa gctcgagcaa ccgctccgca ggaatgacct cgagcctagg
    13261 cacaccattc ttctctcccc taatcttgag ctcgtgaatg tgaagcatct gatcttcgtc
    13321 aagggctacc accaacgagc cggtgttctt gtagagaacc cctagttcct gagtgatctt
    13381 tggataaaga gcattgccgg ccacgttcag cttggccttc aaagtccctg gatccgcgtc
    13441 ataaccagca tgaacaaggg ccgtattggc cttggtggtg ccaaaggcca catctgcctc
    13501 tttctcaacc aacacaacac tcaactcata tttggaaagc tcgcgggcta tggcagtgcc
    13561 tacaatgccc gcgccgataa caacaacatc tgctgtaatc cgtttcgttg ccgccatatc
    13621 gttaccccct agattttcca cctcaacacg gggaaatact ctgctcccaa ttgcgcgaac
    13681 gctccaccgc cctcttccaa cccgcataaa gcttttcacg ttcaccatgg cacatctttg
    13741 gggtaaactc ccgatcccgt tgccatgtgc acgccagctc gtcctggccg ttccaaaagc
    13801 ccactcccaa tcctgccagg caggcagcgc ccaacgcggt agtctcaatc accttgggcc
    13861 tggccacagg gactccgaga atatcagatt ggaactgcat catgaagttg ttcgccaccg
    13921 ctccgccgtc cactttgagc tgcttaagct ctatcccgct gtcggcagtc atagcctcaa
    13981 gcacgtcacg cgtctgatag cagatggcct ccagagccgc cctgactatg tgatcgcgct
    14041 ctgtgcctcg ggtaatcccc actattgttc cgcgtgcgaa cggatcccag tgaggagccc
    14101 caagccctac gaacgcaggg accacataca cgccgccagt atcggccacg cgattggcgc
    14161 attcctcgga ttcggctgca gaatcgatta ttcgcaaccc gtccctgagc cattggacca
    14221 ccgcgccgcc aataaagacg ctgccctcaa gtgcatactc aaccctgcca tcgatgcccc
    14281 acgcaatagt agtgaggaga ttgttcctgg acatgactac atcctcgccc gtgttcatca
    14341 gcatgaagca acctgtcccg tacgtgtttt tcgcactgcc cggcgaaaaa catgcctgcc
    14401 cgaaaagggc agcttgctgg tcgccagcta cgccagcaat aggaatctcc gccccaagca
    14461 gactaggttg ggtatagccg aagactccgg tagaaggcct gacttccgga agcatagccc
    14521 gaggaatatc gaggatttcc agaatctcat cgtcccagtc gagtgtatga atgttgaaaa
    14581 gcatagtgcg cgacgcatta gaataatccg tggcatgtac tcggccgcct gttagattcc
    14641 atatgagcca tgaatccaca gtcccgaaga ggatctcccc tcgctctgct ctgcctcgaa
    14701 ggtcgggcgc attgtcgagg atccacttga tcttggtggc agaaaagtaa gcatctacga
    14761 taagcccggt cttctcatga atcgctttgg cttcgccagc agctttcaac tcctcgcagc
    14821 agcccgcagt cctcctgcat tgccagacta ttgcgttgcc gataggacag ccattcgact
    14881 tgtcccacaa tatcgtcgtc tcccgctgat tggttatccc tatagccgct atctcatcag
    14941 gaccaacacc ggtggaatcg ataacacccc ttgcagtttt cagctgagtt ctccagatct
    15001 cgttcgggtc gtgttccacc cagccaggct tgggataaat ctgcttgaat tctctgctgg
    15061 caacgccgac actccgcccc aagtggtcga aaagaattgc acgcgaacta gtcgtcccct
    15121 ggtcaagcgc gagaatgtat tcccccattc gatacacctc cgcatcgata ggcccactgt
    15181 ttagaccatc atattgagct atgaatatgg cctaagcgag caaaaagaaa agcccttcat
    15241 cgagcagaag ccatgtaagc tccgtcccgc tgtaggactc tccaaatctc cgtcctattt
    15301 aacatgatcc cttttccctt ttccgactac gacagtataa taacacatgc ccacctgctt
    15361 ttctacccaa agggccaaaa atcctctata cacgacaggt tatccccaaa aacagaatat
    15421 cgtatcatgc cacgcgcctt cacaatcccc aagaggccca accgcgtcaa aagccgattc
    15481 attcagcatc ttacagatga tgctccgagc aaatcagcat agcccacttt catggccaat
    15541 cccgtcatca tccccatccg ccattggcca tcggcggaac ttgctagctt taatagatat
    15601 tccgctttaa gctggcaaac aggatgccca ctcccatgag actatttatc catgcattcc
    15661 aattcagtcc agagttgaca catgagtcca taagtagtag tgttaaggta cgtacatccg
    15721 tattattagt accaatttgt tgggccctgc ccacagtctg caaaggctta tgcccgagtt
    15781 ttagcaaaac agtgctctcc ccattaatta tcgagtggtg agtatcatgt cgaaagcgtc
    15841 ttctctcgaa aagtcgcttg ccaagaagct caatcgcgac atctcatgct acgaaaaaga
    15901 cggcgccata gtcctggaag gaaatgtgcc cacatgggat catgttgttg ctgccggcaa
    15961 aagcggagca aagctaggct acaagggtgt agtcaaccgc ctgacttgcg atggaattct
    16021 ggccatgccc ggccccaaag cgccttccat tcgagacgcg tccctggaag gagagcgcat
    16081 ggacgttctc gttatcggcg gcggagtgat cggcaccgca atcttaagag agctctccag
    16141 atgggatatt cgagcatgtc tcatagaaaa agaagccgac ctcgccatgc atcagagcgg
    16201 cagaaacgcc ggcatgatcc atccggccct ggcgccctcc cccggttcga agaaagcgca
    16261 attcaactca ttggggaatc cgatgatccc ggccatcgcg gatgaactag gtgtcccttt
    16321 cagatacaac ggcatgacga taatcgtaga acggcgaata tatggaatcg cttatcccgt
    16381 tctcgccaac agggcgcgac gaaaccgcgc aggtgagatc agatttgtgc ccccagccga
    16441 actacaatcc atggagccta acgtcaggga cgacatcgga ggcgccttct tcatgccgca
    16501 aagcggaatc atagaccctt tcatgctcac aatagcattt gctgaaaatg cagttgaaaa
    16561 cggcgctacc gtctggctaa acaccatggc agaatcattc atcgttgaac acggaaggat
    16621 tctggcagtt gtcaccaatc ggggtgtcct ctttcccaag gcagtagtga acgccgccgg
    16681 gctatgggcg gacacggttg ccgaggccgc aggcgacatg ttcttcacaa tccatcctag
    16741 gcatggcgaa atgctgatcc tcgacaagaa gaaaggcaac ctggcaagat ccaccctctc
    16801 catgccccct gtctatcagg cgcgcactga caccaagggc ggtggcgtga ttcccaccat
    16861 cgatggcaac atcctgattg ggcccgatgc cgtcgaattg ccctatcgcg aacattacgc
    16921 gactagagcg gaacgaaccg agaacctcgt caaaaagcaa caaagattca tcaacggaat
    16981 atcagcatct gatgtgatca cctattttgc cggcacgagg gcagcaacat atgaggaaga
    17041 cttcgtcatc gaacggtcag aacgcgtgcg caacctggtg catgcagcag gaatacagtc
    17101 gccgggccta acctcatcgc ctgccatcgc gcatgaggtt gcatcgatgt gcatcgatgt
    17161 gctcaaagag acgcatgaag tcgaagtcag gcccaacgcc aagttcaacc ctcgcaggaa
    17221 cgccccccct gatttcgccc gactcccgct ggaagaacgc cagagaatta tcaaggcaaa
    17281 cccggattac ggcagggttg tatgcagatg cgaggttatc acggaaggcg agattcgggc
    17341 cgcgcttcgc tctcctgttc cccccacaac ccttgacggg ttaaagcgga gggtcaggac
    17401 tggcatggga cggtgtcagg gcggattctg cacccccctg gtactcgata tcgcctgcga
    17461 tgaaacgggg atcgccccag ataagttcac caataagggc ggagcctcgt atctgctatc
    17521 cagtcccaca cgcccggaac tggaggaggc aatcagtgtc aatccccgca gagacgcacc
    17581 gatcgggcat gcggaagtgc tctctgcgac aaacgatgaa gactgcactc cacaggcttt
    17641 gggagcgtat gacgtagtga tcatcggagg agggccggcc gggctcgctg cagccattgc
    17701 cgcacatgac gcaggcgcca aagtcggact catagaacgc gaagcgcgcc tcggcggcat
    17761 cctaaagcaa tgcgtccacg acggcttcgg aacgatcgag ttcaacacgc atctgactgg
    17821 cccagagtac gccgaacgct tcatgtcgca ggcagcccag cgggacatta catgttacac
    17881 gcaaacgttc gtgctcgatg tagcccgcaa tggagatgga cgcaaaccag ataacttcag
    17941 gccctcggaa ggccgcaatt ttgcacttac tgtggaaaac cgggacggaa tattcaacat
    18001 tgaatcgaag gcattggtcc tagccacagg gtgcagagaa cgcacagctc gacaagtctt
    18061 catccacggg acgaaacctg ccggcatata cagtgcaggt acagcccagt atatggtgaa
    18121 cgtgcaaggg cggctgcccg gccgcaggtg cgtaatactt ggaagcggcg atgtgggcct
    18181 cataatggct cgcagactga cactggaggg tgccacggtg gtggctgttt atgaagccaa
    18241 atcagagccg acgggccttc cccggaacat ctcccaatgc ctagatgact tcggcatccc
    18301 tctttatctc tctcacaccg taacaaaggt tttcggcgac gaacgcgttg aggcgataga
    18361 gatcgcccaa gttgacggcg aaatgaagcc gatcgtcggg acagagcaaa gaatcgaatg
    18421 cgactgcgtc atcgtcgcag ttggattgat tcccgaaaac gagctggccg aaacgctggg
    18481 agccgaaatt tccaacacaa ctcgcggtcc caaggttgat cagacgttcc aaaccactat
    18541 tccaggactc tttgcctgcg gcaactgcgt tcacgttcac gacctcgtcg attacgtaac
    18601 acaaagcggc agagtggccg ggctgagtgc cgcccaatac gcccaaggag agcgaagaat
    18661 tggccagata acccatgtat gcccctctga cgatctcctc tattccgttc cccaattcat
    18721 cgacgccgaa atggcaaatg aagccttcac gatctacttc agatcacgag gcacacacca
    18781 aaatgtcgtg gtccggctaa cccaaagcgg caaggtcctc gccgaatcga agtatcccgt
    18841 ggtgcgatcc cagcagatgc aggccatagg ggtgaaggcc acgaacgcaa gcttcgatcc
    18901 atatgcccca ctctctttgg agctggcgat gcgccaatcg aacatgggag aggagtgatc
    18961 attatgacag ctcaaagcgt tggcaaaggc caaatatccc aacacaaggc ctttacatgc
    19021 atagtctgtc cgcgcagttg cgatcttaca gctgaactag tggaagacgg ctggagaata
    19081 tcggggaaca ggtgcccaat gggtagggat tttgccctaa aagaagtaaa caatcccacg
    19141 agagtcgtgt gttctactgc gagaacgact gaccgatcct gtccgctcct tcccgtaagg
    19201 accgacatcg aagtgccgct ggaggacgtt ccccgtgtaa tggcagagat catcaaggct
    19261 gttgtagatg tgagcactgg agtggacatg ggagacgtca ttatccacaa cgtggcaggc
    19321 actggcgcga acgtaatagc gtcggcaagc gctactatgc caatgggggt atcctcaatt
    19381 gaaacaagga ctgatcgttt ccattgattg cggaacccaa agcgcacgag cgctgctatt
    19441 tgattgtaga ggaagcctgg tggcaaaggg caaggcggag tttgaaccct acttttctac
    19501 gcagccaggc tgggcagagc aagatcctga agtctatttc gacgccatct gcaaggcatg
    19561 ccatgacctg gcatcaagcg ctcctgatga ttggggccgc attgtaggag tagtcctgac
    19621 cacccaacgc gatacctgcg tagtcgttga cgagaatggc aatgtgctga gaccggccat
    19681 gctctggctc gatcagcgaa tggccgaatg caacaagccc tatcctttta tctacagact
    19741 cgccttcggc gcagtaggga tgctcgaaac agcggagctc actcgcaaga aatgcaagtc
    19801 acagtggatc cgtgaaaatg agccggatat ttgggacaag atggacaagt tcctgctcct
    19861 ttccggatgg ctcaactaca agctcaccgg aaacatggcg gactcggttg caaaccagat
    19921 aggacacgtt ccattcgact acaaacacaa aaggtggccc gtttcgaagt tagactacag
    19981 atggtacatg agtggtttga gccggcgaca ccttcctcag ctcgtaaggc ccgcgcaagt
    20041 gataggcacc ataacgcctg aagcagcaga ggccactggg ctgccagtag gcaccccggt
    20101 atatgccggg ggatctgaca aaggatgcga aaccctcgga actggatgcc tcaatccaac
    20161 tagtgcatgc atcagccttg gaactacagc aacattgcag ataactaccg aaaagtacat
    20221 ggaaccgatc aagttcatgc cgccttaccc tgcagtagtg ccaggacact tcaaccctga
    20281 agtgcagata taccgcggct attggatgat caactggttc aagcaagaat tcggataccg
    20341 cgaagtgctc atagcagaac agcgtggagt cgctcctgag gtaatcctaa acgagctgct
    20401 ccatgagact ccgccgggct gcatgggcct gatgctccag ccatactggg gcgcggggct
    20461 caggatgcca gaagcccgcg gcgccgtaat cgggtttgga gacattcaca cgcgtgccca
    20521 tctgtatcga tcgataatag aaggcgtagc atacgctcta cgagatgcgg ctgacaaggt
    20581 tgaaaaagtc tccggcacaa agatagaacg gataatgata tcgggcggcg gcgcgcaaac
    20641 agactccata tgccagataa cagcggatgt attcaacaag cctgtgttgc gcggcgaaac
    20701 ctacgaggga tctggacttg gcgctgcaat cataggattc tgcggcgcgg gatattacag
    20761 cagcttcgat gatgcagttg ccaacatggt tcactacgaa gcccgcttcc aaccagatcc
    20821 accgactgca agaacatatg accagctata taagtgtgta tacaagaaaa tgtattcgcg
    20881 cctcaagccg ctctatgagg aaatgcgcgc gattaccgga tatcccgaaa gctaggaaga
    20941 ggtgatcaat tgtgagcaac aagaaaccgt tcgagcctga ttggtatgaa gggccaatcc
    21001 ctccaaagtc atatagatcg atattcaagt ggggaggcgc cgatgaatac aagcacccaa
    21061 accttcatct ctatgagctt atgaaggaag tattcgagat gaccgatgag gattttcgcg
    21121 ccccggtcaa cttagggctt gaggaggtca gttttgatgt accgataacg ctctccggcg
    21181 aacacctcaa agccatcaca aatatcgtcg gaacggagaa tgtcgcgaca gacgactact
    21241 cgcgactcaa agcttcatat ggcaaaatga tctacgacaa catacgtctg cgtgaaggaa
    21301 tcgtcgagaa cctcccggat atcgtcgtat atcccaggca caaacaggat gtgtcggaga
    21361 tcataggcta ctgcaacagg cataaaatag cagtctatcc gcggggcggc gggagcggag
    21421 tcatgcgggc caccgaatgc gtacatggcg gaatatctct ggatctcacg aagcacatga
    21481 accgcatcct caccctcaat gaggtaaacc acactatcac agtagaacct ggcatatacc
    21541 ttcccgacct tgaagcgcga ctgaataagg cacccgagtt cttcggagcc tccaggcgct
    21601 ataccgcggg ttatctgcct cagtccttca taggcgccac cgtcggcggt tcagttgtga
    21661 cccggggagc agggcagaac tcaacatatt acggcaagat agaagacctc gtcctctgtc
    21721 aggagtatgt cacccctgcg ggagatataa aaacaaagga gttcccgaga gcggcggttg
    21781 gaccttccat cgaccagata atgataggca gcgaaggcac atacggggtt ctcgtatccg
    21841 ttaccctgaa gatattcagg tatacgccgg aaacaacaca gcgtttcagt tacatcttcc
    21901 caacgtggag caatggcctc gcagcatccc gcgaaatgat gcaaagcagg ttcggatatc
    21961 cgtcggtgct ccggatctcc gacccagaag agactgatgt ggcattaaag ctgtatggag
    22021 tagaagacac tatcctcgat aagatgatgg atgctcgtgg gtacaagcgt atggagcgtt
    22081 gcctaatgct ggggatgtct gagggtgaag agaatttcgc caagaacgta aaaaggcaaa
    22141 taaacaggat agcgaaaaga tacggcgcga tgaataccac gggatacgtc gccaagtcat
    22201 gggaaaaagg ccgtttcacc gatcccttca tgcgggagga cctccatgat tatggaatca
    22261 tcattgatac tttggaatgc gccgtcgcat gggataagat cgaatccttg tgggctgaga
    22321 ccagaaaggt ctgcaaggcc aggccgaaaa cggtagtaat gagccactcg tcccacttct
    22381 accctcaagg aacgaacctc tatttcattt ttatcggacg ttttgagact atcgatgaat
    22441 acctcgatta ccaagccagc atcattgata gcatacgggc caacggcgga gcgctcagcc
    22501 atcaccatgg gatcggaaag atgctggcgc catggtacca agacgcaatg ggtgaaaacc
    22561 acgttgccgt gctcagagca ttgaagcggc attttgaccc aaacaacatc atgaaccctg
    22621 gaggaaccct ggccctcgat ctagaagaat cgttccgcag accgattgag tagggcatta
    22681 tgtgcccttc ggggctagtg agcagtatgg cgagggttgt gcatgataga caaactaagc
    22741 ccagtccctt tttatatcca attgagggat gggatcaagc ggaacgtgat tgaaggagtt
    22801 tggaaagtgg gagagcagat tcctaccgag aacgagctga tggagaagta tgaagtcagt
    22861 cgtgccacgg tgagaaacgc catttccagc ctggtcaatg aaggctttct tgtgaaacag
    22921 cgaggccgcg gaacattcgt cgcgcgccag caaccatcta tggctttcga gcccctgata
    22981 agtttcgcat acaatgtaga gtctatgggg gcttcgagcg aaagccaagt tgtggagaac
    23041 agcatcatag ttcccgacga ggaactcctt cgtttgttga gatggaaaga gcccaaggag
    23101 tgtctttacg tcaagagagt gaggcacgga gacggcattc ccttggcaat tgaagaatcg
    23161 ttcttcaacg attccacagc caagtttctc aagctgaaag atctctcagg atccatatcc
    23221 cgcctcctcc ttgaggaatt gagaatatcg atatcccggg tcgaacagat agtcgctgcc
    23281 cgcaaagcca cagcggaaga gaaaaagctg ctcaacctcg ataaagaacc tcaggtgctt
    23341 gagatggagc gatggataat cctagaagat gaagaagagc ccttttacta tctgagattc
    23401 attatgcgtg gggatctgta ctcgcttaca ccagtgccca ggtgataatc tcggagaggc
    23461 aatgtttcga tgccagcagc aaggcgcctc gcagccctca ctttggggac gcgttggcgc
    23521 ctcttggcgc tctgtgggac gatcacccct gtagcccgtt ttcgacgaag cagcgacatc
    23581 gcctgtaaag cgccgatgcg atgctcaggg gcaagctatg ccgtgggtga acagcgtata
    23641 cacttcgtgg ctcacaacgc ttacggaaac gctcttgcct gcagaaagcc actgatatgc
    23701 tacagaatga atcatgcctt caagggcgaa ggaagctctc attggatcca cgtctgaact
    23761 gattacgccc aaagccatgc cctcctctat cactccgcac accatattga tgagcgctgc
    23821 tgtgccggtc actaatcgct cttccatttc tggagaaagc agtcctagct gccccgaaaa
    23881 gagcagcatg aactcgctat gaatagagta gaactggaaa tgggcctcga taacggcaaa
    23941 aacctttccg aacacgtcct cagccgatgc gatgccattg agtatcatat ccgtcagccg
    24001 tcgcgtccgg tcatcaacaa gctcaatgag gagttcgttc tttcccttga aataaaggta
    24061 gatagtgccc ttggctacac ctgcagcctc ggccaccgca tccattgtgg aagtatgaaa
    24121 gcctttttcg gcaaacacat gttgtgcagc atctagtatc agttggcgct tgtcagccac
    24181 gggcaccata taatcgttgt gcatactgcg cctccctcct caccaccgcc tgcatcctcc
    24241 cgccattcgg attttcgaac actgcaggat aggcaatggc cagttcgatt gttactctag
    24301 attattcccg atctttatat ttcccccttc cacacggcat tcccttgttg catagcacaa
    24361 agagagccga accgttacac cggcacgctc tctttgcggc aacattcgaa agcgcggggg
    24421 cgctccgcca ccggcccgca gacgcctgca tgctatgtgc gattcgccac tgtgtgccca
    24481 agcatctccc ggaaagtctg ctactctaac gaaggccggg caaccatggc tcaaaaggga
    24541 cgccgagaca tcccttat
//
LOCUS       DAOSYL010000036        24435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1691_length_24435_cov_8.319319, whole genome shotgun sequence.
ACCESSION   DAOSYL010000036 DAOSYL010000000
VERSION     DAOSYL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24435
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_24435_cov_8.319319"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..138)
                     /locus_tag="PLU15_07045"
     tRNA            complement(64..138)
                     /locus_tag="PLU15_07045"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Asn,seq:gtt)
     gene            complement(802..1911)
                     /gene="rpoD"
                     /locus_tag="PLU15_07050"
     CDS             complement(802..1911)
                     /gene="rpoD"
                     /locus_tag="PLU15_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOO30717.1"
                     /translation="MTKEFNAPELKNLVAKGKKRGFLTEREIVDALQDVDLTPDQIEE
                     MYEVLGEFGVDIVVGNDSSEADDDVVVDSEDDSADEEEETEIDVSVPEGIALDDPVRM
                     YLKEIGRVPLLTAEEEVELAKRIEQGDEEAKRELAEANLRLVVSIAKRYVGRGMLFLD
                     LIQEGNLGLLKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLVRVQRQLLQELGREPTPEEIAEVMDYPVERVREIMKVAQEPVSLETPIGEEEDSHL
                     GDFIEDEDAIAPPDAASFLLMKEQLEEVLYTLAPREEKVLRLRFGIDDGRARTLEEVG
                     QEFGVTRERIRQIEAKALRKLRHPSRSKKLKDFLE"
     gene            complement(1974..3821)
                     /gene="dnaG"
                     /locus_tag="PLU15_07055"
     CDS             complement(1974..3821)
                     /gene="dnaG"
                     /locus_tag="PLU15_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOO30718.1"
                     /translation="MSSKFSNDLVDKVREANDIVSVVSDYVQLKKVGNRFIGLCPFHS
                     ERTPSFSVSPDRQLFYCFGCHVGGNVFTFIMKMEGLTFPEAVRRLGLRAGIDVSTSVE
                     SPQERAARERRESIYRVNALALAYYRQTLVHSPSAECARDYLKQRRIEADTQEKFKLG
                     YAPASWDSLLTMLAKKGIGPTEVCEAGLALRASGGNRYYDRFRNRLVFPIVNVYGRVV
                     GFGGRALDDSTPKYLNSPESAVFSKRSNLYGIDLASDSMRRSGKAVLVEGYMDVLSAH
                     QAGQTNVVATLGTALTTEQARIVLRYADHVVLCYDADAAGSDATARGVSTTSAVGLDV
                     RVAVLPQGHDPDSFIKEFGGEAFQRSVDEAVPFIRYQLDSLLDAVDWSDLESRLHGLE
                     KVVDVLSGVDSEVERAEYTRELSERFNVDPGALALDVRKARMAAMRGTSRAGPGGNLR
                     SGSRPKPSAWNHGLDDPIWGSKAYLEVERALVRLATESLQNRTRIMETIGPDGFKMPA
                     HQRLFRLLCDVGENGAVTPAELVDCVDAELKDYASSVLFGAKPWESEPDALENALTAW
                     QQFSTRHRLNEIDDEIKEADVAGNYERVYELQAEQKKLRYLLGRDVPLY"
     gene            complement(4107..5129)
                     /locus_tag="PLU15_07060"
     CDS             complement(4107..5129)
                     /locus_tag="PLU15_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOO30719.1"
                     /translation="MTVRERLEEEELERLSPHAAFSRNTRGRDREEEPDEFRTCYQRD
                     RDRIIHCKAFRRLKHKTQVFLSPEGDHYRTRLTHTLEVAQIARTMARALQLNEDLTEA
                     IALGHDLGHTPFGHSGEAALNEVVDGGFRHNEQSLRVVEFVERREGKPGRGLNLTWEV
                     RNGILCHSADWPEEAATLEGQVVKCADKIAYVNHDVDDAMRAGIINEGMIPEEAQRIL
                     GRTRSERLDTLVKDVIDESTNRGKIAMSPEVAQGMAALRNFMFERVYIGSVAKIEADK
                     AKRLLIQLYCYYLDNVDELIEESGESLQGEDVVRIAVDYIAGMTDRYAMKQWTERFVP
                     AAWGVY"
     gene            complement(5268..7919)
                     /gene="ppdK"
                     /locus_tag="PLU15_07065"
     CDS             complement(5268..7919)
                     /gene="ppdK"
                     /locus_tag="PLU15_07065"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOO30720.1"
                     /translation="MGKNNMPKYVYYFGGKQADGKGDMKNLLGGKGAGLAEMTNLGIP
                     VPPGFTITTEVCTAFYENDRKWPEGLEEQIRENLKRLEEEMGAKFADPDNPLLLSVRS
                     GARVSMPGMMDTVLNLGLNDATAQGLIKKTGNPRFVYDSYRRFVHMYSDVVLEVPHEA
                     FEEAIAAKKKARGVKLDMDLTADDLKELVEEYKEIARKHTGKDFPEDPWEQLRGSINA
                     VFDSWNNQRAITYRKINGIPGDWGTAVNVQTMVFGNMGETSGTGVAFTRDPATGENVF
                     YGEYLMNAQGEDVVAGIRTPKPISELKKSMPEIYAQLEQIYHTLEKHYKDMQDIEFTI
                     QDGRLFMLQTRNGKRTGTAAVRIAVEMVEEGLIDKKTAVLRVPPAQLDQLLHPMIDAK
                     AKVDVIAKGLPASPGAAVGRVVFTAKAAEEWAARGEKVVLARTETSPEDIGGMHVAVG
                     ILTSRGGMTSHAAVVARGMGTCCVAGCGALIINEKEKRITCGDLTISEGDWITLNGST
                     GEVILGQVPLVEPELTGNFGKLMEWADEFRALKVRTNADTPEDAKQARYFGAEGIGLC
                     RTEHMFFAEDRIRAMREMILADTEEQRRTALAKLLPYQKGDFMGIFREMDGLPVTIRL
                     LDPPLHEFLPSLEQSKQIEELASEMGIAEQKVRDRIGALHELNPMLGFRGCRLGVVFS
                     EIYEMQTRAIFEAACELVREGVNVMPEVMVPLVGTTGEMEMIREYVVGIADKTIKEYG
                     VDLDYMVGTMIEVPRAALIADEIAKSAEFFSFGTNDMTQMVFGYSRDDAGKFLGEYMD
                     RNVLKHDPFQSLDQEGVGQMVTMGVERGRSTRPDLKIGVCGEHGGDPDSVEFFHKAGL
                     DYVSCSPYRVPIARLAAAQAEIKQPRK"
     gene            complement(8007..10118)
                     /gene="glyS"
                     /locus_tag="PLU15_07070"
     CDS             complement(8007..10118)
                     /gene="glyS"
                     /locus_tag="PLU15_07070"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOO30721.1"
                     /translation="MVQSDRVGAVLEIGTEELPARFMPGILEDARTTAASVLEEARLD
                     VGRVRTYATPRRIAIILDDVAPSQADLVREVRGPATRIAYDEDGNPTRAAEGFARSAG
                     ISVAELVRKETPQGEYVYAIVREDGCDAATVLSEAFPRMIDSISFPKSMRWGWGELCF
                     ARPIRWILALLGKEVVDFEYHGMSSGRVTWGHRTLAPGPVEIVSSSEYPARLREIGVM
                     LDPQERASTIMSQAQSLAAECGGTPVVEEGLLEEVTFLVEWPTAFAGRFSSDYLALPE
                     PCVITPMVGHQRYFPVRDADGHLMPVFIGVRNGDDYRLDVVAAGNERVLAARLADARF
                     FYDDDMKVPLAERVPHLKNVVFQEKLGTMYDKAERIEVLGRSLISDSSDADILKRAAF
                     LAKADLVTAMVREFTELQGVMGREYALRQGEDEKTAQAIFEHYLPRYAGDILPSTRVG
                     AALSIADKMDTVAGYFAIGLIPSGSADPYALRRQAAGMLAVHAEWRFSKGLDEIARLA
                     VSLYEEAGVVQCSTEQLVKDVLEFLGTRLKVSLLESGIRHDIADAAISSGVGRPACVR
                     EKAYAVASVVDQDWFVRLATAATRVKNIAGRSEPGKCDPELFDDPAEQGLYDAVCKLE
                     ADLGVQEKEDASGLVERDMYIDAMKRTADICDDIDRYFDAVLVMADDDRVRKNRLAML
                     KWVLDIVSSVIDTTQIVFPGN"
     gene            complement(10105..10974)
                     /locus_tag="PLU15_07075"
     CDS             complement(10105..10974)
                     /locus_tag="PLU15_07075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOO30722.1"
                     /translation="MNFQDLIFALEQYWARQGATIQQPYVIEVGAGTMSPHTFLRVLG
                     PKPWKVAYVQPSRRPADGRYGENPNRLYQHHQYQVILKPNPDDVVDLYLGSLSAMGIN
                     PKEHDIRLVEDNWEAPTLGAWGTGWEIWLDGMEITQFTYFQQVGGIDTRPVSVELTYG
                     LERISMYLQGVDNVYDMMWADGIAYGDMYHRAEYEFSKYAFEYADIGILSDSFDNYER
                     EAVRCIGAGLLLPGYDYVLKCSHVFNVLDARGAFGAAQRAEYMGRVRALARKVAVAWV
                     RSEEEEAAENGSE"
     gene            complement(10999..11655)
                     /locus_tag="PLU15_07080"
     CDS             complement(10999..11655)
                     /locus_tag="PLU15_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOO30723.1"
                     /translation="MEVDFLRMLAGLYTRALPRAFRSYRRYLLFAAMLLVTCMIVGYT
                     GFSPSGDSLPWIFNSLIGLRDSMAGASTWEQIGMLFWNNLKATLVSIGLGWIFGIVPV
                     ISVCLNGVLIGACTKLMVVRNALSASVVIASFLPHGILELPAFLAGQVVGLRLGFLLP
                     VVWRGRASKNDLGRAAVEGAIILGLFCVPLLAIAAVVELKVTPAVVRMFAPELPTLPL
                     "
     gene            complement(11896..12297)
                     /locus_tag="PLU15_07085"
     CDS             complement(11896..12297)
                     /locus_tag="PLU15_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOO30724.1"
                     /translation="MKVDLDKIDKLRARIDLGYDEAAAYLEAAGGDLVQALIMAERDI
                     GSAVDGSLDLDGGQRESTDGSTWTGRLADVLRRGSEMRLVVERDGANVAYMPVSAGLV
                     GAIFAPELAAVGAILALATRCTMRVEPNPNE"
     gene            complement(12294..13061)
                     /gene="recO"
                     /locus_tag="PLU15_07090"
     CDS             complement(12294..13061)
                     /gene="recO"
                     /locus_tag="PLU15_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOO30725.1"
                     /translation="MSKCEGVILRVKDFGEADRILTILSDTDGKFDAVARGARRPRSR
                     LVGVTQQFSRVNALLFRGRNLDTLSTVELLSSYSSITSDVMKMAYASYVAELVDLMTG
                     ERQRNEMLYALVISTFEALDAGPADPDMVLKAFEIKFMSVSGFRPELARCVSCGKPEN
                     QASWFGPEAGGLLCDGCRSSDWGSVRFSAATRELAKRLLIWDYDMIAVLKPSRSSFEE
                     LNNAFRAYVDQRVGRKLHSLDFISDLKSMERGGKQDS"
     gene            complement(13166..13744)
                     /locus_tag="PLU15_07095"
     CDS             complement(13166..13744)
                     /locus_tag="PLU15_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOO30726.1"
                     /translation="MNLLLHSCCAPCTTYCLEKLRSDGHDVYGYFYNPNIHPYVEFRR
                     RLDTYRDYCASVGLDADICESYGLRQYIVEVGSDIDGRCAVCYRMRLLSAAKLALEKG
                     VDAFTTTLSISPYQNHELLARVGEDVSREVGVPFIYYDFRPGYRRSVEISRELGLYRQ
                     PYCGCIFSEEERYSPKFRKSRKRTKKGSADKE"
     gene            complement(13741..14769)
                     /gene="ruvB"
                     /locus_tag="PLU15_07100"
     CDS             complement(13741..14769)
                     /gene="ruvB"
                     /locus_tag="PLU15_07100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOO30727.1"
                     /translation="MEERIVTGEKTDEDRGSELSLRPRRLSEFIGQNRVKESLSIFIE
                     AARSRGEPLDHVLLYGPPGLGKTTLANIISVEMGVRLRVTSGPAIERAGDLAAILTNL
                     APGDVLFVDEIHRLGRPVEEILYPAMEDYALDIIIGKGPSARSIRIDLPRFTLVGATT
                     RTGLISSPLRDRFGVVHRLELYSVDDLATIVSKSAEILGIEIEALGAYEIARRSRGTP
                     RVANRMLRRVRDYAEVRADGVITQDVACDALAMFDVDELGLDRLDRKILATIIDKFGG
                     GPVGVDTIAAAINEERDTIEDVYEPYLMQLGFIDRTPRGRVATSLAYQHIGREFPTDE
                     ADGQARLL"
     gene            complement(14785..15393)
                     /gene="ruvA"
                     /locus_tag="PLU15_07105"
     CDS             complement(14785..15393)
                     /gene="ruvA"
                     /locus_tag="PLU15_07105"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOO30728.1"
                     /translation="MISQLRGKVASIERDAIVLDVGGIGFSVSVTSGTRATLGRIGEE
                     VRLFTAMVIRDDCVNLYGFESVEEREFFHMLCEVKGIGPKTALSILSSLSPETLSMAI
                     SSGDIAMLATAPGVGKRTAERLAVELKGKVGQFISDEFIERGASANADSTAAQDAISA
                     LVSLGYSPVEAERAVAAAVAAGYSDDASAIIRAALSRMSRAE"
     gene            complement(15390..15896)
                     /gene="ruvC"
                     /locus_tag="PLU15_07110"
     CDS             complement(15390..15896)
                     /gene="ruvC"
                     /locus_tag="PLU15_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOO30729.1"
                     /translation="MLVLGIDPGTAITGYGLVQEANGRIECKDMGCVLTRADLPLAVR
                     LSQIYDELFEIIRRDKPDCAAVEQLFFNTNAKSALAVGHARGVALLACEKAGIPVAEY
                     TPLQVKMAVAGYGRADKKQVQSMVKLLLALDSEPKPDDVADALAIAICHLSTAPYLAK
                     ISGDGDRL"
     gene            complement(16036..16800)
                     /locus_tag="PLU15_07115"
     CDS             complement(16036..16800)
                     /locus_tag="PLU15_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO30730.1"
                     /translation="MSGHSKWANIKHKKAKADAQRGAAFTKASRELHVAVKQGGPDPE
                     ANFRLKMAIQAARAVNMPNDTIQRAIKRAAGDGDGESYEEIVYEGYGPGGVALVVEAM
                     TDNRNRTASDVRHIFSRHGGNLGETGCVNWMFDRKGSITVDTESFAGDEDEMMMIALD
                     AGAEDLKAYDDYYEIITSPEDLDAVRKAMESAGVEYSGARIIRVPQNVMVLGTKEAGP
                     AMRLVDALDEHDDVQHVYTNFDIPDEVLEELEKEGA"
     gene            complement(16917..17744)
                     /locus_tag="PLU15_07120"
     CDS             complement(16917..17744)
                     /locus_tag="PLU15_07120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOO30731.1"
                     /translation="MAELLKLLAISLIPGILWVIYFHRKDIYDKEPARLVAKAFLAGA
                     VMIIPAGLVEAPLRGVLTNPPSLPVLLLASVFGIGLVEEFFKYLAVRRTVYDLAEFNE
                     PMDGIVYAVSAGLGFAAFENVLYAASYGLPVGIVRGALTSLVHASFSGIVGFQMGIAK
                     FAPKSMEGPLVARGLMTASVLHGLYDFLLMTELMNFYLTLLVVVLLYVGLAGKIRTAL
                     HVSPFRFAELYGKEDGRSEDSVEIGRIGDAAGTGDPDVPSGNGRVANGEDDSSSSRR"
     gene            17948..18232
                     /gene="groES"
                     /locus_tag="PLU15_07125"
     CDS             17948..18232
                     /gene="groES"
                     /locus_tag="PLU15_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOO30732.1"
                     /translation="MVKPCADRVLVKPHTEEEKTPGGIVLPDTAKERPQWGEVKAVGP
                     GRWDEEGKRRIPLEVNVGDTVIFAKYGGTEIKIDGEEYLILRESDILAVK"
     gene            18296..19915
                     /gene="groL"
                     /locus_tag="PLU15_07130"
     CDS             18296..19915
                     /gene="groL"
                     /locus_tag="PLU15_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392083.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOO30733.1"
                     /translation="MAAKQLAYAEEARTSLKNGVDAVASAVKVTLGPKGRNVVLERKY
                     GSPVITKDGVTVAKEIELEDPYENMGAQLVKEVASKTNDVAGDGTTTATVLAQIIVNE
                     GLRNVTAGANPMFLKRGIDKAVELVVDELHKLAIPVDGKEDIAHVASISGNDEAVGEW
                     IAEAMDKVGKDGVITVEESKGIETTVEQVEGMEFDKGYISPYFVTSTESMEAILEDPY
                     ILLYEKKISNVQELLPLLEKIARAGRPLLIVAEDIEGEALATLVVNKLRGILNVVAVK
                     APGFGDRRNAMMEDIAVLTGGEFISEATGAKLENVDLKQLGQAKTVKVNKEKTTIIEG
                     RGEKAKIEARVDQIRREIDDSDSDYDREKLQERLAKLAGGVAIIKVGASTETELKEKK
                     HRIEDALSATRAAVEEGLVAGGGATYINMISKLDQIEAEGDEKTGVNIVKRALEEPLR
                     TIANNAGLEGSVVVERVKSENKPGFGLNALTGEYCDLIKAGIVDPVKVSRSALQNAAS
                     IASMMLTTEAVIAEIPTEEPPMPYPPGGMDY"
     gene            complement(20231..20305)
                     /locus_tag="PLU15_07135"
     tRNA            complement(20231..20305)
                     /locus_tag="PLU15_07135"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20271..20273),aa:Asn,seq:gtt)
     gene            20377..21513
                     /locus_tag="PLU15_07140"
     CDS             20377..21513
                     /locus_tag="PLU15_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO30734.1"
                     /translation="MNKSQVSITRCKDYEREHVKEALSEALSPLGGMGTFIEPGSRVL
                     IKPNLIAGTPPERGATTHPEVLRAVIEEVRSVGGKPIVGESPGFESFHVAASKSGLLE
                     VLEEMGVDAAPFETSRNVPLSPGHIVTSLPIAEPVVDADAVISLPKLKSHGLVMYTGC
                     VKNMFGVICGSQKGKYHLRFQDRELFSTLLAEIYATAKPTLSILDGIIGMDGNGPRNG
                     DPFDLGIIMAGSDGVAIDAVACSIVGINPVESPPLRIASEKGYGTIDLGNIDIYGPSI
                     EELQVQGFRVPKAARPSMRQRLMSGETGRVLRNWFTTRPSVVRNSCRGCRVCARACPA
                     DAIEIRDGVAHMDYDKCIRCYCCQELCEYGAITLKTPPLARIFR"
     gene            21833..23575
                     /locus_tag="PLU15_07145"
     CDS             21833..23575
                     /locus_tag="PLU15_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOO30735.1"
                     /translation="MVVLKATRLSKTYGIETIFKNASFDISLGERVALFGPNGSGKST
                     LLRLITGDEEPDDGQVWIDPKMRVGYVPQKLEFPESATPLFLCQVASLGLPDVGNAEE
                     ALSRFGISKKMRMRRVGTLSAGERTRLGLACAWVIRPDILLLDEPTCHLDIEGMELLT
                     DLLLSHKGAALIVSHDRRFLDDVAMRVLELAPDGIRSFTGNYSAYRMQKEAELDAQMK
                     AHIHYKREERRLEARISQQMEWFRSAHRSAGQNDFLRAKAKKGARRAKATAKRLERLR
                     KEKVPKPKASESPLMQTTPADKTGVRMILMEGAARAFGSTPLFEDLDLAVSRGDHLGI
                     IGPNGCGKTTLLRTMLGLESLTAGTVWRSPSCRFFYMDQHYETLDPNLTVLEAITEAQ
                     SRGSAPGNDRGKQIQAARDLLASLLFRGADVHKRISVLSWGERARVSIARMLISNYDL
                     IALDEPTNNLDVQSREAIESCLAEWKGTLVVVSHDRRFLEHTCNMIAAFNSGQVSVFP
                     GTYGEWEEHRKLEAAQAIGPAQKQGHTVLELEHRLALLSAKLSEKFQSDEEKVAIEEE
                     FLATARTLNRLRQK"
     gene            complement(23653..24303)
                     /locus_tag="PLU15_07150"
     CDS             complement(23653..24303)
                     /locus_tag="PLU15_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO30736.1"
                     /translation="MEREVREIIEEQLFSSEVILWAGRPRRGLALRASDWFLIPFSLV
                     WLCIATEITGNARKFGVPSVRYEFGLSGRRVFAQPLTVFEVVGWLFIAIGLYLLVGRF
                     FVDARVRRNTYYGVTDRRILILTGFFGNRFVSIPLDRVGEMNAIRRADGSGTIQFGRP
                     TYIPGWGDSTMNGHHYQNWRRVDPPSFEMIEDVLSVRNIIVSAQQGTYDGSGGAGG"
BASE COUNT         5876 a         7306 c         5775 g         5478 t
ORIGIN      
        1 ggattcgaac ctgggaccaa tcggttatga gccgaccgct ctaccgctga gctatgggcc
       61 caatggctcc ccgagcagga ttcgaacctg cgacccaccg gttaacagcc ggttgctcta
      121 ccgctgagct atcggggaac taacttgcaa aaagtattat accgtacgca cattcgaggg
      181 tcaacaccac ataaggcagc atttgcattg cagtcatctt ctcatcaacg atttccggca
      241 tcctaaccgc catttcatct atgagggtat acgcctactt tatctcgctc ctcgatgccc
      301 tcgtaatccg agaggctaca ggcaactcgt aaacggcatc cgcgacgaga tgcagtttat
      361 acccaaacca actcatgatc ctgtccctac gctgccatct ttgctcatat gtcttcactc
      421 cgaaatccgc gtcgatattc ctgcggccat catagctatc gaaactccca tcaacctttt
      481 attgatgcct catcagtttg cacaagaatc tggtgcatgc cgatggcaaa tgaacaaggc
      541 cggcccagtt tcggggctat gtgttccgcc actgccattg acatcttgtc cgaaaagaaa
      601 aggcggcttc ggccccatgc accaccagca tggtcaacac agccgccaaa attcgatctc
      661 cattcctcgt ctggccaaca agccaagcgc caatgcctaa agtgatggca tgccccgcga
      721 tcagcttacc ccttcaacat gccagaccca acggaactaa tacatccgta catctccacc
      781 taaggtgaaa tatgcacaaa cttattctag gaaatccttg agcttcttgc ttcggctcgg
      841 atgtcgcagt ttgcgcaggg ccttggcttc tatctggcgt atgcgctccc tggtcactcc
      901 gaactcctgt ccaacctctt caagcgtccg agcccgaccg tcgtcaattc caaatctcag
      961 tctcagaacc ttctcctcac gcggcgccag agtgtaaagc acctcttcca gttgttcttt
     1021 catgagaagg aacgaagcag catcgggcgg agctatggca tcctcgtctt cgataaagtc
     1081 accaagatgc gaatcctctt cttctccgat gggggtttcc agggaaacag gctcctgcgc
     1141 taccttcatg atctccctta cccgctccac ggggtaatcc atgacctcgg caatctcctc
     1201 cggcgtaggc tcccggccta gctcctgcaa aagttgcctt tggacacgca caagcttgtt
     1261 tatggtctcc accatgtgaa caggaatgcg tatagtcctt gcctgatcag caatggccct
     1321 ggtaatggcc tgccttatcc accaagtggc gtaggtgctg aacttgtaac cctttcgata
     1381 atcgaacttc tccaccgctt tcaagaggcc cagattgccc tcttgtatca ggtcgagaaa
     1441 gagcatgcca cgaccgacat accgtttagc tatgctcaca accaacctca gatttgcctc
     1501 agccaactcg cgttttgcct cttcgtcgcc ttgctctatc ctcttcgcta actctacttc
     1561 ctcttcggca gtgagcaagg gaactctccc gatttccttc agatacatac gtaccggatc
     1621 gtccagggca atcccctcgg gaacagacac gtctatttcg gtttcctctt cctcatcagc
     1681 tgaatcgtct tcagaatcaa caactacgtc gtcatcggcc tcgctactgt cgttgcccac
     1741 cacgatgtct accccgaact cccctagcac ctcgtacatc tcctcgatct gatccggggt
     1801 gaggtctaca tcttgcaacg catccactat ctcacgttca gtgagaaagc ctcgcttctt
     1861 gcctttagcg acgaggttct tcaattcggg cgcgttgaat tcctttgtca tcttttcctc
     1921 accttccgaa tcgccttgcg caaagccgac ctgcttgcca acaaccatag caactaataa
     1981 agagggacat cgcgtccaag caagtaacgc aacttcttct gctctgcctg gagctcatat
     2041 acgcgctcat agttccctgc aacgtcggct tccttgatct cgtcgtcaat ttcattaagc
     2101 cggtgccttg ttgaaaactg ctgccatgct gtgagcgcgt tttctagagc atcaggttct
     2161 gattcccatg gtttcgcgcc aaacagcaca gatgaggcat aatccttcaa ttcagcatcc
     2221 acacaatcca ccagctcggc cggggtcaca gccccattct cgcccacatc acatagcagg
     2281 cgaaacaatc tttgatgtgc tggcatctta aaaccgtctg gtcctattgt ttccataata
     2341 cgcgtccggt tctgaagaga ctctgtcgcc aaacgaacta atgccctttc gacttcaaga
     2401 tacgctttgc tgccccagat cgggtcatca aggccatgat tccatgctga cggcttgggc
     2461 ctcgacccgg aacgcaaatt ccctccaggg cctgcccggc tcgttccacg catggctgcc
     2521 attcgcgcct ttcgaacatc cagagccaga gcacctggat ccacgttaaa cctttctgaa
     2581 agctctcttg tgtattcggc ccgctcgact tcgctgtcaa ctcctgataa aacatccact
     2641 accttctcaa gcccatgcag gcgactttcg aggtcagacc aatcgactgc atccagcaaa
     2701 ctgtcaagtt gataccttat gaacggaacg gcctcatcca cagacctttg aaaggcttcg
     2761 cctccgaatt cctttataaa gctatcagga tcatgcccct ggggtaacac agctacccgc
     2821 acatcaagcc ccacagcgga cgtggtacta acgcctcggg ccgtcgcgtc tgaccctgcc
     2881 gcatcagcat cgtaacagag aactacatgg tccgcgtatc tcaacacaat cctggcctgt
     2941 tcagtagtga gagctgtccc aagggtggcc accacattgg tttggcctgc ctgatgtgca
     3001 gagagcacat ccatgtatcc ttccaccaga acggctttcc cgcttcttct cattgaatca
     3061 gacgccagat cgatcccata caggttcgat ctcttgctga agaccgccga ttcagggctg
     3121 ttcaggtatt tcggtgtcga atcatccagg gctcgaccgc caaatcccac aacccgacca
     3181 tacacattaa cgatgggaaa caccaaacgg ttcctgaatc tgtcataata cctgtttccg
     3241 cccgaagcac gaagggccaa tcctgcctca catacttccg tcggtccgat tcccttcttg
     3301 gcaagcatcg taagaagcga atcccacgag gccggcgcgt agcctagttt gaatttttcc
     3361 tgagtatcgg cttctattcg ccgttgcttc agataatccc ttgcacattc agcagacggc
     3421 gaatgaacta gcgtctgcct atagtatgcc aaggcaagag catttactct gtatatcgac
     3481 tctcttcgct cccgcgctgc ccgctcctgc ggggactcga cgcttgttga tacatctatt
     3541 ccggcacgca ggccgaggcg gcgaactgcc tccgggaacg taagcccttc cattttcatt
     3601 atgaaagtga agacgttccc tcccacatgg cacccgaagc agtagaagag ctgacgatcg
     3661 ggcgacacgg aaaacgatgg cgtccgctca gagtggaacg ggcaaagacc aataaacctg
     3721 ttccccacct tcttgagctg cacgtaatct gacacaactg atacaatgtc atttgcctct
     3781 cttaccttgt cgaccaagtc attactgaac tttgaactca aaccaaccag cccctgccgg
     3841 ggtacttttc gccggcgata gatattctcc tgccagatgc ctgtttagtg gcgtcaaaca
     3901 aaaccccgct tccgaatctg ctcctggcgg aagcgagggc atccgaaccc agtgaccaca
     3961 agaccacttg cacatccgta attctaccta tgatcagatt tcccttccta tgatcttcaa
     4021 tctttcccca tggcgggcag acgatctacg ccgagtataa tgtctcctaa tgcacccagc
     4081 ttatgtagga cgatcattcg actgtctcaa tagacccccc acgcagcagg aacaaatctc
     4141 tctgtccatt gcttcattgc atatctatct gtcatgcctg cgatgtaatc tacggcgatc
     4201 ctgaccacat cctcaccctg caatgattcc ccggactcct ctatcagttc atctacattg
     4261 tcaagataat agcaataaag ctggattagg agccttttcg ccttatccgc ctcgatcttg
     4321 gcaaccgatc cgatgtatac cctctcaaac atgaaattcc gcaatgcggc catgccctga
     4381 gcaacttcag ggctcatcgc aatcttgcct ctattcgtgc tctcgtcaat gacgtccttt
     4441 acgagcgtgt caaggcgctc agatctcgtg cgtcccagga tccgctgcgc ctcctctgga
     4501 atcatgcctt cgttgatgat tccagcgcgc atagcatcat ccacatcgtg gttaacatac
     4561 gcgatcttgt cggcgcactt tacgacctgt ccctcaagtg tagcagcctc ttccggccaa
     4621 tccgccgagt ggcaaagtat cccgttgcgg acctcccatg ttaggttaag ccctcgcccg
     4681 ggcttgcctt cccttcgctc aacaaactct accactctca aactttgctc gttgtggcga
     4741 aacccgccat ccacaacctc attgagggca gcctcgccag aatgtccgaa tggcgtatgc
     4801 cccaagtcat gcccaagagc tatggcttca gtaaggtctt cattgagttg gagcgcccgt
     4861 gccatggtcc gagcaatctg ggcaacctcg agggtatgag ttagccgtgt tcgatagtgg
     4921 tctccttcag gagagagaaa aacctgtgtc ttgtgtttca gcctccgaaa agccttgcaa
     4981 tgtatgatcc tgtccctgtc tcgctggtag caggtgcgaa attcatccgg ctcctcttcg
     5041 cgatcacgcc cgcgagtatt gcgcgagaat gcggcatgcg gtgaaagcct ctccagttcc
     5101 tcttcttcca gacgctctct gaccgtcatg gcacactcct cctccctatg attattgccg
     5161 taagtggccc acgcggcatc tccgccacgt gggccacaga ctactctcag atagggagct
     5221 ccgtctcgta agttgaagtc atcctgttgg gtttcgctcc gcgctcccta ctttcttggc
     5281 tgcttgatct cggcctgtgc tgcagcaagg cgcgcgatcg gcacacgata aggagaacag
     5341 ctaacgtaat caagcccggc cttgtggaag aactccacgg aatcagggtc tccaccgtgc
     5401 tcaccacata cgcctatttt cagatcaggc ctggtagacc gtccgcgctc aacacccatg
     5461 gttaccatct ggcccacgcc ttcctggtcc agcgactgga acgggtcatg tttcaaaacg
     5521 ttgcggtcca tgtattcgcc gaggaacttc cctgcgtcgt cccggctgta tccgaagacc
     5581 atctgcgtca tgtcattggt accgaaggag aagaactcag cagacttcgc tatctcgtca
     5641 gcgatcaacg cagcccgcgg aacctcgatc atcgttccga ccatgtagtc aaggtcgact
     5701 ccatattcct tgatcgtctt gtccgctatg ccgacaacat attcgcgtat catctccatc
     5761 tcgcctgtgg tcccaacgag aggcaccatt acctcgggca ttacatttac cccttcgcga
     5821 actagctcgc acgcagcctc gaatatggcc cttgtctgca tttcatagat ctcggagaac
     5881 acaacaccaa gtcggcagcc tctgaacccc aacatcggat tgagctcgtg cagagcgccg
     5941 atcctgtctc gcactttctg ctcggcgatc cccatctcag acgccaattc ctcgatctgc
     6001 ttgctctgct cgaggcttgg caggaactcg tgaaggggtg gatcgagcaa cctgatggtt
     6061 acgggcagac catccatttc ccggaaaatc cccatgaagt cgcctttttg gtacgggagc
     6121 agcttggcaa gagctgtgcg cctctgctct tcagtgtcag caaggatcat ctcgcgcatg
     6181 gcgcgtatcc tatcctcggc gaagaacatg tgctcagttc ggcacagtcc gattccctca
     6241 gccccaaaat accgagcctg cttggcatct tccggcgtat ccgcattcgt tcggaccttc
     6301 aaagcgcgga attcatcggc ccactccatg agcttcccga agtttcctgt aagctcgggc
     6361 tccaccaacg gaacctgccc caatatgact tcacctgtgg atccgttcag agtgatccaa
     6421 tcgccttctg aaatagtaag atcgccacag gttattctct tctccttctc attgatgatg
     6481 agagcaccgc aacctgccac acaacacgtt cccatgcctc tggcaacaac ggctgcatgg
     6541 ctcgtcattc cgcctcgact ggtaagtatg ccaacggcaa catgcatgcc gccgatatct
     6601 tcaggcgatg tctcggtcct agcaaggact accttctccc cacgagccgc ccattcctcc
     6661 gcagccttag cggtgaagac aactcttccg actgcagcgc ctggcgatgc tggcaagccc
     6721 ttggctatca catcgacctt ggccttagcg tcgatcatcg ggtgcagcag ctgatcgagc
     6781 tgggctggcg gaactcggag aaccgctgtc ttcttgtcta tcaagccttc ctcgaccatt
     6841 tctactgcaa tgcggactgc agctgttccg gttctcttgc cgtttctcgt ctggagcatg
     6901 aagagacgac catcctgaat cgtgaactct atatcctgca tatctttgta gtgtttttca
     6961 agggtatgat atatctgctc caactgggca tatatctccg gcatggactt tttcaactcg
     7021 ctgatgggtt tgggagtcct tatacctgca acaacatcct cgccctgagc attcatgagg
     7081 tactcgccat agaagacatt ttcgcctgtt gcagggtcac gcgtaaaggc gactcccgta
     7141 cctgaagttt ctcccatgtt tccaaagacc atggtttgga cgtttaccgc agttccccaa
     7201 tccccaggga ttccgtttat cttgcgataa gttattgcac gctggttatt ccacgaatcg
     7261 aagactgcgt tgatcgatcc tcgcagctgt tcccaaggat cttcagggaa atccttgcct
     7321 gtgtgtttgc gcgctatttc cttgtactct tccaccagtt ccttcaggtc gtccgcagtg
     7381 agatccatgt cgagcttgac tccgcgagcc tttttcttag cagctattgc ctcctcaaag
     7441 gcttcgtggg gaacctccaa gacgacatcg gaatacatgt gcacaaaccg tcgatagctg
     7501 tcgtagacga accgaggatt tcctgttttc ttgatgagcc cttgggcagt agcatcgttc
     7561 aaaccgagat tgaggactgt gtccatcatg cctggcattg aaacacgggc accagacctt
     7621 actgaaagca aaagcggatt atccggatcg gcgaacttgg cacccatctc ctcttcgagg
     7681 cgcttgagat tctcccgaat ctgttcctct agcccttcgg gccatttccg atcgttctca
     7741 taaaaagcag tgcagacctc cgtggtgata gtaaaaccgg gcgggacagg aatccccaag
     7801 ttggtcattt cagcaagacc cgctcccttg ccgcccagga ggttcttcat atcgccttta
     7861 ccgtcggcct gtttacctcc gaagtagtag acatactttg gcatgttatt ctttcccatg
     7921 ctaatccccc tatctaatgc agcaatctac gcatcaaatg gcattgaatt ctactcaaac
     7981 tctctttttc cttcttctgc gcccggctaa tttccgggga agactatctg tgtcgtgtca
     8041 atgaccgaag atacgatatc taaaacccat ttgagcattg caaggcggtt cttcctgact
     8101 cggtcatcat ccgccatgac caacaccgca tcgaaatatc gatcgatgtc gtcacagata
     8161 tcagccgttc tcttcatggc atctatatac atatcccttt ccaccaaacc agacgcatct
     8221 tccttctcct gcacgcccaa atccgcctcg agcttgcaaa cagcgtcata caggccttgt
     8281 tcagcaggat catcaaagag ttccgggtcg cacttgccag gctcagaacg cccagctatg
     8341 tttttgactc gcgtcgcagc agtagccaaa cgcacaaacc agtcctgatc gactacagac
     8401 gccaccgcat acgccttctc acgaacgcaa gccggccgcc ccacacccga agaaatggca
     8461 gcgtctgcaa tgtcatggcg tatccccgat tccagtaacg ataccttgag cctcgtgcct
     8521 aggaattcga gaacatcctt tacaagctgc tcagtggagc actgcacaac cccggcctcc
     8581 tcgtacaggc tgaccgcaag ccgcgcaatc tcatccaggc ctttggaaaa cctccactca
     8641 gcatggaccg cgagcatgcc cgcggcttgc cttctgagcg cgtacggatc tgccgatccg
     8701 gacgggatga gcccgatcgc aaagtagccc gcaacggtat ccatcttgtc ggctatgctc
     8761 agcgcagcgc ccacacgggt gctgggcaga atgtcccctg cgtatctcgg caagtagtgc
     8821 tcgaatattg cctgagcggt tttctcgtcc tcaccttgcc gtagagcgta ttccctgccc
     8881 attacgcctt gaagctccgt aaactcacga accatcgctg taaccaggtc agccttggcc
     8941 aagaacgccg cccgcttgag aatatctgca tcactagaat cagatatcaa gctgcgtccg
     9001 agaacctcga tgcgttccgc cttatcatac attgtaccca gtttctcctg aaagaccacg
     9061 ttcttcaggt gcggcaccct ctccgcaagc ggcaccttca tgtcgtcgtc gtagaagaaa
     9121 cgcgcatctg ccagcctcgc agcgagcacc cgctcgttcc cggccgccac cacgtcaaga
     9181 cggtagtcat cgccattacg cactccaata aacacaggca tcagatgccc atcggcgtcc
     9241 cgcaccggga aataacgctg atgtcccacc atcggtgtta taacgcatgg ctcaggaagc
     9301 gcaaggtaat cgctggagaa ccttcctgcg aaggccgttg gccactcaac tagaaacgtg
     9361 acctcttcca gaaggccctc ttccacgaca ggggttcccc cacattccgc cgccagcgac
     9421 tgcgcctggc tcatgattgt tgaagcccgc tcctggggat ctagcattac tccgatctcc
     9481 cgaagccgcg ccggatactc cgagctcgaa acgatctcca cagggcctgg cgccagcgtc
     9541 cggtgtcccc atgtaactcg tccactggac ataccgtgat attcgaaatc cacaacttcc
     9601 ttgcctaaca aagccaatat ccatctgatg ggccgcgcaa aacacagttc cccccaaccc
     9661 catctcatcg atttcggaaa gctgatcgaa tcgatcatcc tggggaacgc ttcactgagc
     9721 actgtcgccg cgtcacatcc atcttcgcgg actatggcgt atacatattc accttgcgga
     9781 gtctctttcc gcaccaattc agccacagag attccggcag agcgtgcgaa tccttcagca
     9841 gcgcgagtgg gattcccgtc ttcatcgtaa gcaatgcggg tcgcaggacc gcgaacctca
     9901 cgcacgaggt cggcctggga tggcgctaca tcgtcaagga ttattgcaat cctacgcggg
     9961 gtggcataag tgcgaacccg accaacatca agacgggcct cttcaagaac cgaagcagct
    10021 gttgttctgg cgtcttccag gattcctggc ataaacctcg cgggcagctc ctcagtgcct
    10081 atctcgagaa ctgccccgac gcgatcactc tgaaccattc tctgccgcct cctcttcctc
    10141 agaccttacc catgccactg caaccttcct tgccaaagct cggacacgtc ccatatactc
    10201 cgcgcgttgg gcagccccaa aagcgcccct tgcatcaagc acgttgaaga catgcgaaca
    10261 tttcagcaca taatcatacc cgggaagcaa caggcccgcc ccgatacatc gtacagcctc
    10321 gcgttcgtag ttgtcgaagg aatcgctgag gattccgata tccgcatact cgaaagcata
    10381 cttggaaaac tcatattcag ctctatgata catatcgcca taagcaatac catcagccca
    10441 catcatatcg taaacgttat ccacaccctg gagatacatg gaaatccttt cgaggccgta
    10501 tgtgagttcc actgaaacag gcctcgtatc gatcccacct acctgttgaa agtaagtgaa
    10561 ctgcgttatc tccatcccgt caagccatat ctcccacccg gtgccccagg cacccaaggt
    10621 aggagcctcc cagttgtctt ccaccagtcg aatatcatgt tctttgggat tgatgcccat
    10681 cgccgacagg ctccccagat acagatcgac cacatcatcg ggattcggct tcaggatcac
    10741 ctggtattga tggtgctgat aaagtctgtt ggggttctct ccgtaccggc catcagcagg
    10801 cctgcgcgac ggttgtacat acgcaacctt ccatggcttt ggccccaaaa ccctcagaaa
    10861 ggtgtggggg ctcatagtgc ctgcgcccac ctctatcacg taaggttgtt gaatcgtggc
    10921 gccctggcgc gcccagtatt gttccaaagc aaatatcaaa tcctgaaagt tcaagcgcgg
    10981 tccctcccca tctcatcgtc atagaggcag cgttggaagc tccggagcaa acatccgaac
    11041 aactgccggc gtaaccttga gctccacaac agcagctatc gccagcagcg gcacacaaaa
    11101 caagccaagg atgatcgctc cctcaacagc cgcgcgcccc agatcgttct tgcttgcgcg
    11161 cccacgccac accaccggaa gaagaaagcc caaacgcaac cccacaacct ggcctgccaa
    11221 gaaagccggc aactccagta ttccatgggg aagaaaagac gcaatcacaa cagatgccga
    11281 tagggcattt ctgacaacca tcagcttcgt acacgccccg atgagcactc cgttaaggca
    11341 tacacttatt acggggacga tgccgaatat ccaccccaga ccgatggaga caagcgtggc
    11401 ttttaggttg ttccaaaaca gcattccaat ctgttcccat gtagaagcgc cggccatgga
    11461 atccctcagc cctatgaggc tgttgaatat ccacggcaga gaatcgcctg aagggctgaa
    11521 tccggtgtaa cccacaatca tgcaggtgac aaggagcata gcggcaaaga gcaagtatcg
    11581 ccggtaactg cgaaacgccc ggggcagtgc ccgagtgtaa aggccggcaa gcatcctcaa
    11641 aaagtccacc tccatgaaaa aaagcctccg cccccgaaag ctcgtggggg gcacgggagg
    11701 ctatgttccc gaagcgccgg gcgcgttcgc ttgcacgctg tcatttgccc taactatagc
    11761 aaatggccct gcaatatgtc aattcccggc tctggtttta gcaccttccc tcgtacgccg
    11821 aaaaaagctt gcattagata gtcctcccgc taacgtggat gtaccccgaa cccggaagga
    11881 tgatgctcga tccgctcact cgttcggatt cggctctacc ctcatcgtgc atctggtcgc
    11941 cagggccaag atggccccca cggctgccag ctcgggagcg aaaatagccc ctaccaatcc
    12001 tgccgacacc ggcatataag ctacattggc cccgtcgcgt tccaccacca gcctcatctc
    12061 agaaccacgt ctgagaacgt ccgccaggcg tccggtccat gtcgaaccgt ccgtggactc
    12121 acgttggcca ccgtccaaat ccaggctgcc atccacagcg cttccaatgt ctctttccgc
    12181 cattatgaga gcctgaacaa gatcgcctcc tgctgcttcc aaatacgcag cggcctcatc
    12241 atagccgaga tcaatccgcg cgcggagctt gtcaatttta tcgaggtcaa ccttcaacta
    12301 tcctgtttgc cccctcgctc catcgattta agatcagaga tgaagtcaag cgaatgcagc
    12361 ttgcgcccta cccgctgatc cacataggcc ctgaaggcat tgtttagttc ttcaaagctg
    12421 ctacgactcg gcttgagcac cgctatcatg tcgtaatccc atatcagaag gcgcttggcc
    12481 agttcccgcg ttgcagcgga aaatcggacg cttccccagt ccgaactgcg gcacccatcg
    12541 cacagcaacc ccccggcctc cgggccaaac catgacgctt ggttttcagg ctttccacag
    12601 gaaacacatc ttgcaagctc aggtcggaag ccagaaacag acatgaactt tatttcaaac
    12661 gccttaagca ccatatcagg gtctgcgggc cccgcatcga gcgcttcgaa tgtcgaaata
    12721 accaaggcgt atagcatttc gttacgctgc ctttcgcctg tcatgagatc gaccagttca
    12781 gcaacgtatg aagcatatgc catcttcatt acatccgatg tgatgctgga ataagaagac
    12841 agcagctcca cagtgctcag cgtatccaag tttctgccac ggaacaatag cgcattcacg
    12901 cgcgaaaatt gttgagtaac cccaaccagt ctgctccgag ggcgccgagc gccgcgggcg
    12961 acagcatcaa acttcccatc cgtatcagaa aggatcgtta agatccggtc tgcttcaccg
    13021 aaatctttaa cccgaagaat aaccccttca cattttgaca acgccaatcc gcatcatcct
    13081 caacatgcgg accaccgggg ctttgtaatg taacgttccc atcggcgctc cgcgctatgc
    13141 aaggaatacg actgctcccg aaggtttact ccttatcagc agagcctttt ttcgtccgct
    13201 tacgcgactt gcggaacttt ggggagtagc gctcttcctc actgaatatg cagccacaat
    13261 acggctgtcg ataaaggccc aattctcgcg agatttcaac gcttcgccta tatccaggcc
    13321 tgaaatcata atagataaag ggcactccga cttccctcga cacgtcctca cccacccgcg
    13381 ctaaaagctc gtggttttga taggggctga tagaaagagt tgtagtaaat gcgtcaacac
    13441 ccttctccag tgcaagcttt gccgcggaaa ggaggcgcat gcgatagcag acggcgcatc
    13501 tgccgtcgat atcactccca acctccacaa tgtactgccg aaggccataa ctctcacaaa
    13561 tatccgcgtc cagaccaact gatgcacagt aatccctgta tgtgtccagc cttcgccgaa
    13621 actcgacata cggatgtatg ttggggttat agaaataccc gtatacatca tgcccatcag
    13681 acctcagctt ctcgaggcaa taggtagtgc acggggcaca gcaagaatgg agcagcaaat
    13741 tcacagcaac ctcgcctgcc catctgcttc atctgttgga aattcccgtc ctatgtgctg
    13801 gtatgccaag cttgtagcca ctcgaccgcg gggcgtcctg tctatgaaac ctagctgcat
    13861 caagtacggc tcatacacat cctcgatagt atctctctcc tcattgatgg cagccgcgat
    13921 agtgtccact ccgaccggtc cacccccgaa cttgtctatt atagttgcta gaatcttcct
    13981 gtcaagcctg tcgaggccaa gttcatccac atcaaacatg gccaaggcat cacaagccac
    14041 atcctgtgtt atgacgccgt ctgcgcgcac ctcggcgtaa tcgcgtaccc gcctcagcat
    14101 cctattggcc accctagggg ttcccctgga ccgcctcgct atctcgtaag cccctaaggc
    14161 ctcgatctca attcccaaga tctcagcgga ttttgagacg attgtggcaa gatcgtctac
    14221 agagtatagc tctagcctgt ggactactcc aaacctatcg cgaaggggcg aggatatcag
    14281 tcctgtccta gttgtggccc ccaccagagt aaatcttggc aggtcgatcc gtatcgatct
    14341 tgcagacggg cctttgccga tgatgatatc gagcgcataa tcctccatgg ccgggtagag
    14401 tatctcctca accggccttc ccagcctgtg gatctcatcc acgaacagca cgtccccggg
    14461 ggcaagattg gtaagtatgg cagccaagtc ccccgcgcgt tctatagccg gcccagacgt
    14521 gacccgaagc cttacaccca tctccactga gatgatgttg gccaacgtcg tttttccgag
    14581 gcctggggga ccatacagca gcacgtgatc gagaggctcg cccctacttc tggcggcttc
    14641 tatgaaaatc gagaggctct cctttactct gttttggcca atgaactcag agagccgcct
    14701 tggacggagg ctcaactcag acccgcgatc ttcatctgtc ttttcccccg tcactattct
    14761 ttcctccata gaaatgccct ccgatcattc cgcgcgcgac attcttgaga gcgccgcgcg
    14821 aataatggca gaagcatcgt ccgaatagcc ggctgccacg gctgcagcca ccgccctttc
    14881 ggcttcaaca ggtgagtagc ctagcgatac aagcgcactg atcgcatcct gggcagcagt
    14941 tgaatctgcg tttgccgatg cgcctctctc tatgaattcg tcggagatga actgcccaac
    15001 cttacctttc agttcaactg caagtcgctc cgccgtcctc tttcccaccc cgggagctgt
    15061 agccaacatc gcgatgtcac ccgaagagat agccatggaa agcgtctccg gagaaagcga
    15121 ggataggatc gacagcgcag ttttagggcc aatccccttg acctcgcaga gcatgtggaa
    15181 gaattctcgc tcctccacac tctcaaaacc ataaaggttc acacagtcat cgcgaatcac
    15241 cattgcggta aaaaggcgaa cttcctcccc tattcgcccc aaagtagctc tcgttcccga
    15301 cgtcaccgag acagagaacc caatgccccc aacatccaaa acaatggcgt cccgctcgat
    15361 cgaagccacc ttgccgcgaa gctgagatat catagacgat caccatcccc ggagatcttt
    15421 gcaagatacg gagcggtaga tagatggcat atggctatcg ccagcgcatc tgcaacatca
    15481 tccggctttg gttccgaatc caacgccagc aaaagcttga ccatgctctg cacctgcttc
    15541 ttgtctgccc tgccataccc agcaactgcc attttcacct gaagaggagt atactccgcc
    15601 accggaatgc ccgctttctc acacgctaaa agcgcgacgc ctcgcgcgtg tcctaccgcc
    15661 aaggcgctct tggcgttagt attaaagaaa agttgctcaa ccgcagcgca atccggctta
    15721 tcacgcctga taatctcaaa aagttcatca taaatctgag aaagcctgac agcaagaggg
    15781 aggtctgccc gagtaagcac gcaccccata tctttgcatt caattctgcc atttgcttcc
    15841 tgcaccaggc catagcctgt aatagccgtc ccgggatcga tgccaagcac gagcaccatc
    15901 gccacctccc attcatagca actaataacc gtgagctaag gcaagcccgt ccgcggcata
    15961 ttgcaaacat gcacgccaca tagaaaagcg gccacgcctc gtgcgtggtc gccatgggaa
    16021 ggaagaagca cttgctcaag ctccttcctt ctctagctcc tcaagcactt cgtcgggaat
    16081 gtcgaaattc gtgtacacgt gctgcacatc atcgtgctca tcgagggcgt caacgagacg
    16141 cattgccggg ccagcctctt tggttccaag aaccataaca ttttgcggaa ctcgaattat
    16201 ccgagcacca gagtattcga ctccagcact ctccatggcc ttccgcaccg cgtcaagatc
    16261 ctcgggcgag gtgatgattt catagtaatc gtcgtaagcc ttgagatcct cagctcccgc
    16321 atcaagcgcg atcatcatca tctcgtcttc gtcaccagca aaactttccg tatccacagt
    16381 tatcgagcct ttcctgtcga acatccagtt cacgcatcct gtttccccaa gattgccgcc
    16441 gtgacgcgaa aagatgtgac gaacatcgga tgcggtgcga ttcctgttgt cagtcatagc
    16501 ctctaccacg agagcaactc cacccggccc atatccttca tacacgatct cctcatagct
    16561 ctcgccatct ccatcgcccg ccgccctctt aatggcccgc tgaattgtat cattgggcat
    16621 gttaaccgct cgcgcagcct gaatggccat cttgagcctg aagtttgcct ctggatcagg
    16681 cccaccttgt ttaacggcga catgcagttc gcgcgatgcc ttagtgaaag cagcaccccg
    16741 ctgagcgtca gccttagcct ttttatgctt aatgttagcc catttcgaat gtcctgacat
    16801 catgcgtacc cccttccaca ggaactccaa tgactttgcc gcgtacattc taacacagcc
    16861 ctagctcgtt ggaaaggatt cggcatcaga gcataggaag aagagacatg ccgccctcat
    16921 ctacgactgc tcgagctgtc gtcttctccg tttgccactc ttccattacc agacggcaca
    16981 tccggatcac cggtcccagc agcgtcgcca atccgtccaa tctccacaga atcctcagat
    17041 cttccatcct ctttcccata cagttctgcg aacctgaacg gtgaaacgtg gagagctgtc
    17101 cttatcttcc cggcgagccc gacatatagc aagacaacca cgagcaaagt aaggtagaaa
    17161 ttcatcagct cggtcatgag aagaaaatca tacaaaccat gaagcaccga cgcggtcatc
    17221 aatcctctgg ctacgagcgg tccttccatg gacttcggag cgaacttggc aatacccatt
    17281 tgaaagccga cgatcccgga aaaggatgcg tgcaccaagg atgtaagcgc acctctgact
    17341 atcccaactg gaaggccata ggatgccgcg tagagaacat tctcaaaggc ggcaaatccg
    17401 agcccggccg agaccgcgta gactatgcca tccattggtt cgttgaactc ggcaagatcg
    17461 tacactgttc tccgaacggc caaatacttg aagaactcct cgaccaagcc tattccgaaa
    17521 acagacgcga gcaaaagaac aggcaaactc ggaggattgg taagcacgcc tcgtagaggc
    17581 gcttccacaa gacctgccgg gattatcatt accgcccctg caagaaatgc ctttgcgacc
    17641 aaacgtgctg gctctttgtc gtatatgtct ttccgatgga agtagataac ccataggata
    17701 ccgggaatca gcgatatcgc aagaagcttt agaagttcag ccatggtcca aatcccatcc
    17761 ttcatcggtt cggttaagta ccagtatttc cggacaaacg gcagtcgtcg actgtatata
    17821 gactcctatc ccggaaataa ttctatcata agccttgaca acaccaaaac gaggagtata
    17881 tcatatcttt gttagcactc acatccgttg agtgctaaga gcaacgccgt gaaggaggta
    17941 aacgtcgatg gtaaaacctt gtgctgacag agtgcttgta aagcctcata cggaagaaga
    18001 gaagactccg ggcggaattg ttcttccgga taccgctaaa gagcgccctc agtggggcga
    18061 agtgaaggct gttgggcccg gcagatggga tgaagaaggc aagcgccgca tcccactgga
    18121 ggtaaacgtt ggggacacgg tcatattcgc caaatacggc ggcactgaaa tcaagattga
    18181 cggcgaagaa tacttgattc tccgcgagtc cgatattctc gcggtcaagt agaacctact
    18241 gccccacgaa gaccaacatg caaatcattc atgcgtatga aggaggcaaa cgaaaatggc
    18301 tgctaagcag ttagcatatg cagaagaagc gcgtactagc ctgaaaaacg gtgtcgacgc
    18361 agttgcatct gcagtcaagg tcacattggg tcctaaagga cgcaacgtcg tgcttgagcg
    18421 gaagtatgga tcaccagtca tcactaagga cggcgtgacg gtggccaaag aaatcgagct
    18481 agaggatccg tatgaaaaca tgggagccca gctcgtcaag gaagttgcat cgaaaacgaa
    18541 cgacgtcgca ggcgacggca ccacaaccgc cactgtcctt gcccaaataa ttgtgaacga
    18601 ggggctgcgc aacgtaactg ctggcgcgaa cccgatgttc ctgaagcgcg gtatagacaa
    18661 ggccgtagaa ctcgtcgtag acgagctaca taaactcgcc atccccgtgg acggcaagga
    18721 agacattgcc cacgtagcct cgatttccgg gaatgacgag gccgtcggcg aatggatcgc
    18781 tgaagccatg gacaaagtcg gcaaggacgg agtaatcacc gtcgaggagt ccaagggcat
    18841 cgagaccact gtcgaacagg tcgaaggcat ggaattcgat aagggataca tatcccccta
    18901 cttcgtcact agcacggagt cgatggaggc cattctcgaa gatccatata ttctgcttta
    18961 cgagaagaag atcagtaatg ttcaagagct cctcccattg ctggagaaga tcgcccgtgc
    19021 tggcagaccg cttttgatcg ttgccgaaga tatagaaggc gaagcccttg caactctcgt
    19081 cgtcaacaag ctccgcggaa tcctaaacgt tgtcgctgtt aaggcgccag ggtttggtga
    19141 caggcgcaac gcaatgatgg aagacatagc tgtgcttaca ggcggagaat tcatctcaga
    19201 ggcaacgggt gcaaagcttg aaaacgtcga cctgaagcag ctcggccagg ccaagacggt
    19261 caaggtcaac aaggaaaaga cgaccatcat cgagggccgt ggagagaagg ccaagatcga
    19321 ggcgcgtgtc gaccagattc gccgcgaaat cgacgattcc gattccgact acgaccgtga
    19381 gaagctccag gagcgcctcg caaagctcgc tggcggcgtg gctattatta aagttggcgc
    19441 ctcaacagag accgagctta aggagaagaa gcatagaata gaagatgctc tttcggcaac
    19501 aagggcggcc gttgaggaag gactagttgc aggcggcggc gccacctaca tcaacatgat
    19561 ttccaaactc gatcagatag aagcagaagg cgatgagaag accggagtca atatcgtcaa
    19621 gcgcgctctc gaggaaccgt tgcgcaccat cgccaacaat gccggccttg agggctctgt
    19681 tgtagtcgag agagtgaaat cagagaacaa gcccgggttc ggcttgaacg ctctgactgg
    19741 ggaatactgc gatctaatca aggcaggaat agtcgacccc gtgaaggtca gcaggtcagc
    19801 cctgcagaac gcagcctcca ttgcatcgat gatgctcacc accgaggctg tcatcgccga
    19861 gattcccacg gaagagccgc cgatgccgta tcctccggga ggaatggact actagacgtt
    19921 cgccaatact aaaacaagcg cgggcattcc ctgcaatacc gggatagccc gcgtcctctt
    19981 ttcattcacg cccttttctt cctgtggtat catataagca ttccataggc atggaaccca
    20041 gatttctgcc catctgcaag tatattgccg attacataga gcatggcccc tttggcgcca
    20101 ttcatttcgc tgaattgagc cgctggactt cgccatgttc atctcttgcg gccccaacct
    20161 tgctggggtt gaagcgtaac aggtgggcct ctgtggaagg agaaactacg gacggaaatg
    20221 tacaaaaaaa tggctccccg agcaggattc gaacctgcga cccaccggtt aacagccggt
    20281 tgctctaccg ctgagctatc gaggaacgtt atgaatttat aatagcagcc caacgaagtg
    20341 tagtcaagag gcaaaactag ttacggaggt actgcattga acaagtctca agtttccatc
    20401 acaagatgca aggactatga gcgagagcac gtaaaagaag ccctcagtga agcgctctcg
    20461 cctctcggcg gcatgggcac gtttatcgag cccggcagca gagttttgat taagcccaac
    20521 cttattgccg ggacgcctcc agagcgcgga gctactacac acccagaggt tctcagggcg
    20581 gtaatagagg aagtgagatc ggtcggcggc aaaccaatag tcggcgagag ccccgggttc
    20641 gaaagcttcc atgtggccgc cagcaagtcc ggactcctag aagtgcttga agagatgggt
    20701 gtagatgcgg ctccgttcga aacgtcaagg aatgtgccgc tcagccccgg ccacatagta
    20761 acatccctgc ccatagccga gccggtagtt gacgccgacg cagtaatcag tcttccaaag
    20821 ctgaaatccc acgggctcgt catgtataca gggtgcgtaa agaacatgtt cggagtaata
    20881 tgcggatccc agaagggcaa gtatcatctg aggtttcaag atcgagagct gttttcgaca
    20941 cttctcgccg aaatttacgc cacagccaaa ccgacgcttt caatactcga cggtataatc
    21001 ggcatggacg ggaacggccc gcgaaacgga gacccattcg atctcgggat tattatggca
    21061 ggatcggacg gggtagccat agacgctgtt gcgtgctcaa ttgtcggcat caaccccgtc
    21121 gaatcgccgc cgcttcgcat cgcttcagag aagggctacg gaaccataga tctgggcaac
    21181 atcgatattt acggcccgtc cattgaagaa ctgcaagtgc aagggtttcg ggttcccaag
    21241 gccgcccgtc cttccatgcg gcaaaggctg atgtcgggcg agacaggccg ggtactacgg
    21301 aactggttta cgactcggcc ctcagtagtt cgcaattcgt gccgtggatg cagagtctgc
    21361 gccagggcat gcccagctga cgccattgag atacgcgatg gtgtagcaca catggattac
    21421 gacaagtgca tccggtgtta ctgctgtcag gagctatgcg agtatggagc cattactctc
    21481 aagacccctc ccctcgcccg catttttcga tagaagcagt ttacatgaaa tccggtatat
    21541 gctaacatgt attcagttct taacataggc tttgcggaga ggtaacatat acatgttcag
    21601 taagctggca ttctagctta agcgaatacg ctcaatgcac gcagcgccat gggggcctgc
    21661 ggcgtttgag catttgtggg aatgctggca acctcgcaaa aatagcagat tggatggcta
    21721 cacccgaagg cttgcagcat cggtgtagcc attttgtttc cgcaaagccc tatgctcgtg
    21781 agagtgccca gtcccacttg ccatggaatt catgggtttg ggagtggata ctatggttgt
    21841 tttaaaggca acccgccttt caaaaaccta cggaatagag acaatattca agaacgcctc
    21901 gtttgacatc tctttaggtg agcgtgtagc tttattcggc ccaaacggat caggcaagag
    21961 cacgttgctt cgcttgataa caggagatga ggagcccgat gacgggcagg tgtggatcga
    22021 cccaaaaatg cgtgtgggat atgttcccca aaagctagag tttccagagt cggcaacccc
    22081 tctcttccta tgccaagttg cgagtttggg gctacctgat gttggaaatg ccgaagaggc
    22141 tctgtcacga tttggaattt caaagaagat gcggatgcgg cgcgtaggaa ccttgagcgc
    22201 aggagaacgg actcgtctgg gactagcttg cgcttgggta atccgtcccg atatcctgct
    22261 actggatgag cccacttgtc acctggatat cgaagggatg gaactgttga ccgatctctt
    22321 actgtcgcac aagggcgctg ctctgatagt ctctcacgac cgccgtttcc tagatgacgt
    22381 agcaatgcgc gtcctggagc tagcgccaga tggaattcgt tcattcacag gcaattacag
    22441 tgcataccga atgcaaaaag aagctgagct tgatgcccaa atgaaagcgc acatccatta
    22501 caagcgtgaa gaacgacgct tagaagccag gatatctcag cagatggaat ggttccgatc
    22561 ggcccatcga agtgccggac agaacgattt tctgagagcc aaggccaaaa agggcgcacg
    22621 gagggcaaaa gcgactgcaa aacgcctgga gaggctgcgc aaggagaagg ttccaaaacc
    22681 taaggcatct gaatctccgc tgatgcagac aacgccggcc gacaagaccg gcgttcgcat
    22741 gatactcatg gaaggggcag ctagagcttt tggctcgact ccgctcttcg aagatctgga
    22801 tctagccgtt agccgggggg atcatctggg aatcatcgga cctaatgggt gtggcaagac
    22861 aacgctgctt cgcaccatgc tcggattgga atctctcact gcaggcactg tatggaggag
    22921 cccctcatgc cgttttttct acatggatca acactacgag actcttgacc cgaaccttac
    22981 tgtgctggaa gcgataacgg aggcacagtc tcggggcagc gctcctggca atgacagagg
    23041 aaagcagata caagcggccc gcgatctatt ggcatccctg ctctttcgag gggcagacgt
    23101 tcacaaacgt atatcggtat tgagctgggg tgaacgcgct cgtgtatcaa tcgctagaat
    23161 gcttatatcc aattatgacc taatcgccct cgacgaacct accaataatc ttgacgtgca
    23221 atcccgagag gcaatcgagt catgcctggc agagtggaaa ggaacgctgg tcgtcgtctc
    23281 tcacgacagg cgctttcttg aacacacatg caacatgata gcagccttca attcaggcca
    23341 ggtaagtgtc tttcccggaa catacggcga atgggaggaa cacaggaagc ttgaggctgc
    23401 acaagccatt ggcccagccc aaaagcaggg ccacaccgtg cttgagctcg agcacaggct
    23461 cgcgctgctt tctgcaaagc tatcggagaa attccagtca gatgaggaaa aggtagccat
    23521 agaggaagag tttctggcaa cagcccgaac actcaatagg ctgcgacaga aatagactcc
    23581 ttgcatagaa caggaagacc cggcccgctc atgtgcaacc ttgaagctag ggccgggttc
    23641 ctatgctcgg cgtcagccgc ctgcccctcc cgatccatca tatgtccctt gctgcgctga
    23701 gacgatgatg tttctgacag aaagaacatc ctcaatcatc tcgaacgagg gcgggtccac
    23761 tcgcctccag ttctggtaat ggtggccgtt cattgtagaa tcgccccagc cggggatgta
    23821 tgtaggcctg ccaaactgaa tggttccgct tccgtcagct cttcgaatag cattcatttc
    23881 tccaaccctg tccaatggaa tagatacaaa cctgttgccg aagaaacctg tcaggatgag
    23941 aatccgccta tcggtcacac cgtagtacgt attgcgcctg acccgggcat ccacaaagaa
    24001 gcgcccaacg aggagataca gcccgatggc gatgaacaac catcccacta cctcgaacac
    24061 cgtaagcggc tgcgcaaaca cacgtcggcc tgaaagcccg aattcatatc tcaccgaggg
    24121 gactccaaac tttcttgcat ttcccgtgat ttcagtcgca atgcacaacc aaactagact
    24181 gaatggaatg aggaaccaat ctgaggcccg aagcgccaat cctcttctag ggcgccccgc
    24241 ccacaatatg acctcgcttg aaaacaactg ctcttctatg atctccctga cctcacgctc
    24301 cataatcgtc cccccttcat cacctttgag tatatgaaat attcccggaa gaagccaaaa
    24361 aaggcctcgg catctagcca aggctcttga agataaaaaa gaatccggca gctacctact
    24421 ctcccaggcc gtctc
//
LOCUS       DAOSYL010000037        24403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1694_length_24403_cov_8.496386, whole genome shotgun sequence.
ACCESSION   DAOSYL010000037 DAOSYL010000000
VERSION     DAOSYL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24403
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1694_length_24403_cov_8.496386"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1031)
                     /locus_tag="PLU15_07155"
     CDS             complement(99..1031)
                     /locus_tag="PLU15_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30737.1"
                     /translation="MRMSKAQVVTMIVLCVVLCLGGGYAVGNSSPVERLIGMERLLYG
                     GPQEGPVVARLESIERAIFGEVGPGTLPERLDRCWNFVQGDRAGSMNLKFKLKAVQWT
                     MYGRITDGAIVPMLENIESNLIGQPQTGSIGSRLDSLMQLTIPSGRPNVDYALLPKGS
                     LVKTKLVTAISSVKSRPGDVVRFEVVDDVIFEGRLLMPAGSLLDAIIVETVRPTKIGV
                     RARVDMELYPAEGMDCSPVTLGVDQAAIGANSNMTVVAGDGLRRFSVVGKEGALGGVL
                     SQNPELELPVGAECFFSVMQTAKILGLAVPDARR"
     gene            complement(1069..2520)
                     /locus_tag="PLU15_07160"
     CDS             complement(1069..2520)
                     /locus_tag="PLU15_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30738.1"
                     /translation="MSNHCRAGKIQCFACFILALSVFILAFSAHTIASSPLVPSEDWR
                     YRALAELGRGGLLYEDDAARYASGIPVTEKELSASVERTIYRLNQANGDSTSVVGATA
                     GPLKLSGGPSDTLRLSARQICLVADLVAEFGSSFSDPSLTRLPGSLPLDYEDGLALTL
                     RHATHGNAELHDILKLTANTGSVGYPIRTIEAVTTLDSLEAMLKQAVAHQEPQAQAKM
                     EMGVNSPIGMLAIGLSSEAAQRSTVPARDYSEPATAQGFDLSTSVQLSDILRFTASFA
                     TDDKAVERPTSTAVGVSIGEQEGSGVSVGHRVTDLAGPAPNSLRETITSVDVKYSLPD
                     AGGKASGPDSLTVRAGYELYGREPITLDGEKNSLQATTSLAIDYRLLFGEAASLQAAY
                     RYEHVRDLVASGAVWARDGLYETSLYGWPSEGSLKLVEGSDAVTRTVASIDFSYRLMN
                     DASVMLGYKLIDFTEINALDPKNMATAEVTIRF"
     gene            complement(2603..3004)
                     /gene="ruvX"
                     /locus_tag="PLU15_07165"
     CDS             complement(2603..3004)
                     /gene="ruvX"
                     /locus_tag="PLU15_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOO30739.1"
                     /translation="MDEIVLAVDPGREKAGIAVVSRERGTMHKCVIEASECVEQVCEL
                     AEKHGVARIVIGDRTGSSEIIEALGHVCDLEIIAVDEHMSSMEARERYLRENPGKGIA
                     RFLPIGLRTPKEPYDDYVAEILAARYFDSVS"
     gene            complement(3071..>5253)
                     /locus_tag="PLU15_07170"
     CDS             complement(3071..>5253)
                     /locus_tag="PLU15_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30740.1"
                     /translation="EWQLALDEQNAELMMAFYDSIQAQREDIMAMFEDTESRFELALD
                     ELRAELMMAFYDSVEAEADERARAIDELAEDFDLKLMELHAEVAMETYDSVFAAREEC
                     LAAVDAVVATIDALQAEFSRELAALNLRVDAVERELAVVRGMADQNAKDIAETYHRIK
                     TEDIAPLEERVAKLEGKAAEHDEKISGIWDDLNRFRFTGTNTAQFTDIDLRGDGFYQD
                     PWNPDGKKYNPTSVLQNKLELELNIVPEKGVSIDVGLSAVTDIFGKVDREVGLFDGGL
                     NLDVTTPSGNAKVLAGDLARPRNFTKYQISRKVFGDAGVEGLSLDLTTGPFVTTGMIA
                     KIEPAEDEMQYVLGGGASYTLGSLKVDGRLLKITDDEESAVNAATTPNESVAGAGFAL
                     KLGEGWKLDGEISSWNKDDVATGLLARNLHAAGKAGVLNLTGYFEQVDNGYAPKYLGT
                     VSNDPDRVVANTRKAGFEVKTDSLNGLTITGSIDRAGDEWYASKDVVTYATDAEYEME
                     LGPIDVTLRGGLAQDHNGCPAMDELACATTTDLGFDVAFSPLKAGFTWEHEVEANATS
                     YIGYADLNMPLAADTLTLKGNWKQSFGSNEYYTYGLGIDMKMPITENVSTMGGTMSYG
                     KSSGMARDKDNYARFLVGADLEWKLRPATTFTGKASYELRDYEKDAQPSGEYLQYGLA
                     LSHEIYKSTTLKMNYDFKDVYYSPRNALTNYSARIIGLSLKTVF"
     assembly_gap    5254..5353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5354..6753)
                     /locus_tag="PLU15_07175"
     CDS             complement(<5354..6753)
                     /locus_tag="PLU15_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOO30741.1"
                     /translation="MKKLLAVLVATAMIGIIAIPVLASPFSDLPEESHWALDALTMLA
                     ALGIVEGYPDGSFKGTQPATRYEVALIVARALEYLDKDIRQMSEKMFDLEGRLDAGVV
                     QQEAEPEAEPVLQPSASPDATILEQVIYEKIAAMSDEQWVNFEEKLYELVARVDDLSE
                     DNKAEHAKLWEAIEALKEVRSATVVVSEGADIGLALDSQKAEIMKALSDAVNAEEDER
                     QKAIDALGASMDAALSEQKGEIMMALYDSLEAAKDEGSREMAELEAKLQLALDEQNAA
                     LTMAFYDSLKAAAEGDEQALQGLSGAWQLALDEYNAELTMAFYDSLKASKEETDRAID
                     DVLAKLDMALAEQNAELTMAFYDSIEAAKDEQDRKMADMEAEWQLALDEQNAELMMAF
                     YDSIRAAQNDNAADLEALKAEWQLALDEQNAELMMAFYDSIRAAQNDNAADLEALKAE
                     WQLALDEQNAELMMAF"
     gene            complement(7042..8364)
                     /locus_tag="PLU15_07180"
     CDS             complement(7042..8364)
                     /locus_tag="PLU15_07180"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOO30742.1"
                     /translation="MGGVVLIIVLTIAGGVIAYVGDRIGMKVGRKRLSIFGLRPKYTS
                     MVITIITGVIIAGCTLLVLSLASENVRIALFEIRELQDSLATANNDLEAKRAEVDVLS
                     ARVREVADEYDALESKFEAANRELDRVSHERSRALQELAKVESKLRDSESRLRETETR
                     YKQAGADLARASGELERARDEIASLESEKMSLERNVSSLSAAQASLEKSLEQMKLDMT
                     AQAATSALIMAEAEKSSWYGEVVFRAKEVILGEVVDCSRPRTEVNNQVTEILLKVNAI
                     ASKRGARSSRDDDPFVLLFDEGNYLAAIKDLHEAEGKMIFRVLSYKNSWYGDPLVVEV
                     EVFPDAKIYSAGDVIVSRTINGAAGGAEVQKQLLALMEDVNAACIGKGMTSDEEGRIG
                     TIMTISDFTSAIVEIVKSNANVKVEAVARRDIWRSHKSPPVELVMQGI"
     gene            complement(8393..9112)
                     /gene="lptB"
                     /locus_tag="PLU15_07185"
     CDS             complement(8393..9112)
                     /gene="lptB"
                     /locus_tag="PLU15_07185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOO30743.1"
                     /translation="MGVRATDLVKSFQGRRVVDGASLELERGEVVGLLGPNGAGKTTT
                     FYIMVGLESSDSGCVTIDGVDVVRRPVWQRARLGLGYLAQEPSIFRKLTVEQNITAVL
                     QISTGSSSERTERLESLLEQFDLVGLRRQMGFTLSGGERRRVEIARALAAEPRYILLD
                     EPFTGVDPIAVGELQRIVENLKEAGLGVLITDHNVRDTLAIVDRAYIMHEGRTIVSGT
                     AREVSEDAFARRFYLGERFTL"
     gene            complement(9402..10667)
                     /locus_tag="PLU15_07190"
     CDS             complement(9402..10667)
                     /locus_tag="PLU15_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30744.1"
                     /translation="MDIVLVSSSPEEVHGLARPVAEKALSMLPAADIWMFVSPGGYAS
                     GREAKVASTYPGVRRAVSVREFLAFLAFGKRPSGFASSGSGIVIHLGGNLWCSVLLSR
                     RLRYPLVVYTDRTILGAKTVSAFLVEDERIQASLIAKGVPAERVRLIGNLMVDGVRAG
                     MDRDQARAYLGVANGERLVLLFPGTRVQEAAVMAPFLFRAVELLAKVERDLKFVLCLS
                     PFIGTASLDSIFSYSKHRGKIDGSSAILQKSAGPLEAVTAGGVHVRIETMKRYEAMMA
                     ADIAICTPGPICSELAFLGIPYVAAVPLNVPTAVPFPGVSPFLCSLPFVGELFRKRAV
                     EQVEESGRFLSVPNARAEKLVAPEVVGHLRAEDVVIPCVELLGDTARRDRISRELREA
                     MGVAGAAEMVVRTAIAANRGTLQAIEKGQ"
     gene            complement(10732..11487)
                     /locus_tag="PLU15_07195"
     CDS             complement(10732..11487)
                     /locus_tag="PLU15_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO30745.1"
                     /translation="MRDVKKAYGSRQALSGFDLDVHEGETVVIMGPSGCGKSTALRCI
                     IRLTEPDSGSILWRGRSVLGMSSDELRSYRRRTGFVFQRSNLVSRLSVLENAMLPGVM
                     AGVSIDEAKRLAYGALEKVGIERLAAAYPPNLSGGEMQRAAIARAIAQEPELLLWDEP
                     TASLDPILVVDVLKLIESLARSLGSTMIIVTHEVQFALRVADKVVLVSDGAVVDEGKP
                     EDVLLTPGSDLARQYSESLSYFAGHREEKEVGL"
     gene            complement(11475..13886)
                     /locus_tag="PLU15_07200"
     CDS             complement(11475..13886)
                     /locus_tag="PLU15_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30746.1"
                     /translation="MYALVRGGFILDVAAVLLVTIILGSFVAAGIASAADDFFVHTVS
                     DVVGEYGEYDLIVHVRTESRREALRALRATLRAKLPGARVKESATVLSVSNFLVSLAK
                     KDEESVTALVQGVSGIPGFAGYTFLLEPKVTVSGVGSKTAPIVQDKLTRLDGVEFALR
                     DGGDIHLICESPESVKSVAQAAEAILGRYQVVDIRFAPSALASEGAFRGRLVQRVAAG
                     LAGGDVKGQVVRDVTPRGQADEAQVLAGAMVKMKAFLEAWAPQASIRLDEGKVNPVHT
                     GADTGFSGLDTGDSVVLSVEDKPVGSLPAPEDMVVSVTDVDGDKARGIIVDGAKEPKD
                     QSSQVLSAYLLEDGVVSGRVGSATTSRPQEDLSRSLSEAEEALAQYDAGIADAMRAAK
                     IGEEALESYERAIDSLIYLQRALESIGVDGEADIMPIDDEAVSALLHLTEEALEAIEA
                     LRSTADVVVMFSGSYDSLLADLELWQARLESFAQKLETIQSAATGAGQVAEMLRDMSS
                     AASGILSTLHQLDPASLRENIEAAKVKLAQMSEVDVDGIAEQVREVKSALPKFDGDEI
                     VSSIGAINKLVGDQQGSSDKVQLLIESGLTPDELATAVEEVAGKDVLAVYTIPVGIVQ
                     PGVRSEVQNLLRSVKSTISGLAACGIVAVSLMVDHSTVIAGAIGLERRRQGVLRPGFE
                     EYLYGALAGAISLYAIGAITGSTLGSAGGPLFAAIGGLLGLVGAGVSLKMSPVDLDEV
                     EASIAFGMTGTQIMREIIIPSGKPGILSFINRPHVVFDGIDHVSADRKDRREGGSTRC
                     SQCAM"
     gene            complement(14476..15198)
                     /locus_tag="PLU15_07205"
     CDS             complement(14476..15198)
                     /locus_tag="PLU15_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOO30747.1"
                     /translation="MSLRLATTIEGESGMKGQARRRAMSLVVLAVLLVSAAIVAEAAA
                     KKVNLQVHGKVRYEGSKGRTIVEGGLTIRYDDTVITATNAVIDNAAETAKLTGGVLLT
                     QKDVVLNADEMDVDLKNDTAVVKGNVRLEKREEQAEKDESGKAKVSVVKMTCNILEIS
                     TKTQDFIAQGSVLMTKDKQKAQAQKANYDNAGKTLTLQGSVAIEDEGNKTIKCDRAIL
                     YTDKELVEAEGKPVEITFEIEE"
     gene            complement(15170..15841)
                     /locus_tag="PLU15_07210"
     CDS             complement(15170..15841)
                     /locus_tag="PLU15_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30748.1"
                     /translation="MDPRIVRIVSVAILLVLGCLYVYAFVLNKPADEDRSFDPDPDRA
                     SSDLTFGGVSIEGRENGERVWKISAANVDLRDGDGHVEMSEIGHGEIYRDGEPYVSFR
                     ACKGVFDTKTGQLSLSGGVDVFSGGRKLLRTDEVAWKPDDSRIVIPGQAEIITEQGVV
                     SAASLRVDVASDKIYASGDLTIREGFGGRDVVVKSGEICFDPAENSFEAWGGTEIEFE
                     IGDDD"
     gene            complement(15910..19221)
                     /locus_tag="PLU15_07215"
     CDS             complement(15910..19221)
                     /locus_tag="PLU15_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOO30749.1"
                     /translation="MRILDRYVVHEFMGPFILSILAFIVIMLSGQLFWLVDLIVQKKV
                     PVLIVARMLLYSLPGIVVQVLPVAVLFGVLIGLGRLARDSELQIMRVSGIPFARIALP
                     LIVLGAVMSAVGFWLNESVAPWATHQSEIIVREMIFQEAFPDVEEDVFFKAPGNRYFY
                     VGKADQKRGTFERVMIYETARGEFPRLVSARSGRAESGKWVLEDGSIHELDEEGHVRM
                     AISFETLEISLEQSLDSFASSQKTPEEMSMKELKSNIDLFERSGIRVDALRVDYYLKV
                     AHHVASLVFAVLGAPLVVKSPRRGGGYFGVVAAAVLSFAYYIVQALARSVAMRGGLSP
                     LLAAWLPNIVFGVPGLFMLFAVDRPRMNLAARRRNSGGGDGSAGIKFSRGGVHMWLGV
                     SILTTLYIFAICSPPAVASNIPVKVDAEKLVYDGAIGLWVMSGNVKIEYKDLVISGDY
                     AEFNMDRKVVAVNGSVRVVSGQDALAGESAIFNVSTGEVVVDEAWAKLTGEGVEGFLY
                     MSGSQLQKEDDYLWLSHGSVTTCDLDKPHYRLEVGEMEVYVDDRIVLKSVSYYEGDLK
                     LLTLRRLVVPLKEDERFELPRVGYGAYEGWYLKTKYNYKLSEDAYGSLYADYFQLLGP
                     AVGITQNLQLGKGGVLTAYLYGLWNTGSGTFDTTLELSQDLKLPFDIGAKAGYKQRNY
                     VNILGDEIAERAYNLSLNRRVEDSSTSFAGTFRETDGATYASVANATFRHSSTLLHDI
                     KLSSNVDYKKQVKDTDVTQDVVSYKVQADRAYGDFKVMGLAQAQVYVEDPSDVETKPP
                     PWKALKKLPEFTATASGLAVGESSLILDVKGVFGNYVEDAIRSGTRSEVSAAKGELDL
                     TLRTKSRQLFPWLSMDGYARGVFDVYQGYGDRMAAVCSVGAYVKPFSGTTLRLTYTET
                     GIYGSSPFRFDEINAGSKLGGNLTTSLGPAKLTLSTGYDVVSERYDPLNAALRLDIAK
                     GLSIDFSGRYDLESGNPTSLVGKVVAQPSPDIQVKAGGSYDFVRGQITRVESSGDVRF
                     RPEWRLQWTGVYDFFKGGLVRGDIGITRDLHCREVALTYQHTSRRVWLEFRLKAFPGT
                     PLGFGLGEEGILFQQ"
     gene            complement(19419..19877)
                     /locus_tag="PLU15_07220"
     CDS             complement(19419..19877)
                     /locus_tag="PLU15_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOO30750.1"
                     /translation="MFKNVPKTLLIVLVCAILVGAVAVVANLSAVSRAAGEAAVIGKV
                     DGVRLGQEFPAMQKAVDNILKQKAALEKELNTKAEGQDQETQRTLLDQYNLKYNEYAK
                     QQLEPAENALKSAIAAVAKEKGVTVVLDSSVVHLGGIDMTDAVLKKGGAK"
     gene            complement(19985..21739)
                     /locus_tag="PLU15_07225"
     CDS             complement(19985..21739)
                     /locus_tag="PLU15_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOO30751.1"
                     /translation="MKDTSTKRSCCILAAAMAVVFLASSFTMAAQSKSLGVVTAIEVI
                     GNSRIDSDIITQAINIKVGDELTESAIRESFTAIDALGWFADLGANTVPYLGGVKLII
                     VVQEFPVLSKILVSGNALIPTEELLGIMGSKVGSQLNANQLRSDLEALGSHYHDKGYW
                     VSIEPSLSESGELILHLVEWTVSEVKFAGNQKTKPKVIQRCIETKPGDYINVNKINED
                     RRRIYMLGAFENVEAKVEPVPGKYEYVVTYVLTERKTGIANLALTYSSQDKLMGYMEL
                     GDENFLGNAQTVNIKAEFGRTKTNYELGFYEPWVGASGKTSIGFNVFSKTLSKKYTPS
                     DENETSSSGQVLKLEEDPTASIDYSQKRTGGDVTIGRTLSLNTKAFVKLKMENAVNDP
                     EQKHPDVDFPGGGATRSLTLSAVNDTRDDLWNPATGHKLSSSLEYAGGILGGDNNFAK
                     LRVDACQYYQVRDGHILAGRVSAGYGLDELPDDERFRLGGAETVRGYKYGDIEGDRMA
                     LANAEYRFNITKGLQGVLFCDAGQAWNADGSLDVGSTKVGYGAGVRIMVPVLGMIRVD
                     YGIGEKGGQAYFSFGQTF"
     gene            complement(21876..23924)
                     /locus_tag="PLU15_07230"
     CDS             complement(21876..23924)
                     /locus_tag="PLU15_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30752.1"
                     /translation="MSKSSLVVNVKQKVLIAITVIGVIVSGTMWVRQNLAGMASQAVS
                     LGISRVLGLSDVRIGHVTARGIRSIDLAGVCIGDEVSIDHLLVDYDVSDLFFGRAGLI
                     ESITGVHARGVSVDIAAPLVHEPANRGHVERKSGARSDGGTKTELSRFSAPIEICDVL
                     IEFPSEDQGFRKLEVAKVTVESTLLDGCGVWKVALSNATYTDGTAFDIEGAKAFVDIR
                     GETVELSDIDAQLLGGSVRGRLVLCPSVDGPLFLTGSLDIADLCCGESTISGYVRMDE
                     DGAVAARGSVKLPQAGIDGFLEAEYEGCLRAPGVEIGTRNEARSDARPAAEAGSAYEA
                     HGVLHLVDLSVGEHRASDIDLDLDASYGGGKIKAAIGGERMSMERVGKALGRAIPVEG
                     WVDWKADIEFEDSTLAAVVTIRDGDLRSELWPQEFTDLSGSIEVAEAIPNNGAGSLEE
                     VRVRTIRAAMGGGTVELSGGGQMWTIHGLNLEIEHPLLCGLVDIDATIHTGERMKVGG
                     ILSLCNADVDVVTAGFSKAAVIPDADLDIKIVVGDGVKAVREESWAEIMPSVVQIQGS
                     VRQPRAVGYVQVGPGEVYICDIPLEILSGRVEFANTPDSDIDFSVVARDSSAEFPILA
                     TIYGRPGDVHLSFEPASGVEENFPSGELLMKLLDARLRLYILSRLGQLVGALPQASRD
                     "
BASE COUNT         5671 a         7645 c         5533 g         5454 t
ORIGIN      
        1 tctccatcct tttcgcaagt cattctaaag gctggagttc ccggtctgca tgcctcagca
       61 taggaactcc agcctcatta taccatcagc ctttggcctt atctcctggc atctggaaca
      121 gccagcccca gaatcttagc tgtctgcatc acagagaaga aacactctgc acctacaggc
      181 aactcaagtt cagggttttg actcaagact ccacccagcg cgccttcttt gcccaccacc
      241 gagaacctgc gcaatccgtc tcccgcaacc acagtcatgt tggagttagc gcctatcgcc
      301 gcttggtcca ctccaagagt gacaggagaa cagtccattc cctcagcggg gtagagctcc
      361 atatctaccc tggcccttac gcctatcttc gttggacgca cggtctcgac gattatagca
      421 tcgagcaatg agcctgccgg catcagaagt ctgccctcga atattacatc gtcgactacc
      481 tcaaatctga ccacatctcc cggcctcgac ttgacagagc tgatcgccgt cacaagcttt
      541 gtcttcacaa gagatccctt gggcaacagc gcataatcca cattcggcct tccggaagga
      601 atcgtaagct gcataagcga atcaagccgg cttcctatag atcctgtttg cggctgcccg
      661 atgaggttcg attctatgtt ttctagcatg ggcactatcg cgccatcagt aatccgtccg
      721 tacatcgtcc actgcacggc cttcaacttg aacttcaagt tcatgctgcc tgctcggtcg
      781 ccttgcacga aattccagca cctgtccagc ctttccggaa gcgtcccagg gccaacctct
      841 ccaaatatcg cacgttcgat ggactcaagg cgcgcaacca caggtccctc ttgaggcccg
      901 ccgtatagga gccgttccat gccgataagg cgctccacag gacttgaatt gcctacggca
      961 taacccccgc caagacatag caccacacat aatacaatca tggttacgac ttgagccttc
     1021 gacattctca ttgagctacc tccccttact ggtacgatga aagactcttt agaatctgat
     1081 ggtgacttcg gccgtcgcca tgttcttggg atcgagggca tttatctcag taaaatcgat
     1141 cagcttgtag cctaacatga ccgaagcgtc attcatcaac ctatagctga agtcgatcga
     1201 cgccacagtg cgagttacgg cgtcagaacc ctcaacaagc ttcaatgagc cttcactggg
     1261 ccaaccatac agactcgtct cgtacaggcc atctctcgcc cacactgctc cggatgcaac
     1321 aaggtcgcga acatgttcgt acctgtacgc tgcctgtagt gatgcagcct ccccgaaaag
     1381 cagcctgtaa tcgatggcca aagaagtagt cgcctgcaag ctattcttct caccatcaag
     1441 cgtgataggc tctctgccat agagctcgta gccagccctc acggtcaaag aatcaggccc
     1501 tgaagccttc ccgccggcat cgggaagcga atacttcaca tctaccgatg tgatagtctc
     1561 gcgcaaacta ttcggggcag ggccggcaag atccgttacc ctgtgaccaa cgctcacccc
     1621 ggacccctcc tgctctccta tggaaactcc taccgctgtg gatgtaggcc tctcaacagc
     1681 cttgtcgtcc gtcgcaaagc ttgcggtgaa tcgaaggata tcggataact gcacgctggt
     1741 agacaaatca aaaccctgag cagtcgcggg ctcggagtag tcccgggccg gcacagtcga
     1801 acgttgagcg gcctcactcg acagcccgat cgccaacatt ccaatcgggc tgttgactcc
     1861 catctccatc ttggcttgag cctgaggctc ctgatgtgca accgcctgct taagcatagc
     1921 ctccaggcta tccaacgttg tgaccgcttc tatggttctg ataggatagc caacactgcc
     1981 tgtgttcgcc gttagcttga gaatatcatg cagctcagca tttccgtgcg tagcgtggcg
     2041 aagcgtgagc gccagcccgt cctcgtagtc tagtggaagg cttcctggaa gcctcgtcag
     2101 ggacgggtcc gagaagctcg atccgaattc cgccacgaga tctgcaacaa gacagatctg
     2161 cctggctgat agcctcagcg tatccgacgg gccgcctgaa agtttcaaag gcccagctgt
     2221 agcgcccact accgatgtgg aatcgccatt agcctggttc agccggtaaa tggtcctttc
     2281 aacactggca gacagctctt tttcggtgac gggaataccg ctcgcatatc gggcagcgtc
     2341 atcttcgtac agaagccccc cgcgccccag ttccgcaagg gctctgtatc tccagtcctc
     2401 agaaggaacc aggggcgacg aggcgatcgt atgagcagaa aacgcaagaa tgaagacgga
     2461 taaagcaagg atgaaacagg caaagcactg tatcttccca gcccgacaat gattgctcat
     2521 acttctttcg ccccctctgc tctacactgc gctatcctcc gcagtttgta ctatattctt
     2581 cacaaatcct acgattcctc tcctatgata cactgtcaaa atatcgagcc gcaaggatct
     2641 cggctacata atcgtcataa ggttctttcg gggtcctcag cccgatcggc aaaaagcgag
     2701 cgattccctt tccaggattc tctcgaagat agcgttcccg cgcctccata gaggacatgt
     2761 gctcgtcgac ggcgataatt tccaggtcgc aaacatgtcc taatgcctct attatttcac
     2821 tcgatccagt cctgtcccca atcacgatcc tcgcaacgcc atgtttttcg gccaactcgc
     2881 acacttgctc cacacattcc gacgcctcta tcacgcattt atgcatcgtc ccgcgttccc
     2941 gtgatactac tgcaattcct gctttctccc gccctgggtc tacagcaagc actatctcgt
     3001 ccacagcaca tcacctccta tgaaaatgcc ccgggggaca tacgctcccc ggggcatcca
     3061 acggctattc ttagaatacc gtcttcaagg ataggcctat gatcctcgcg gagtaattcg
     3121 tcaaggcgtt ccttggcgaa tagtagacgt ccttgaagtc gtagttcatc ttgagcgtag
     3181 tggacttgta gatctcatgc gagagcgcca acccgtactg tagatactcg cctgaaggct
     3241 gcgcatcctt ttcgtagtcg cgcagctcgt aggaggcttt ccctgtaaag gtagtcgccg
     3301 gccgcagttt ccattcgagg tctgcgccca ccaggaatct cgcgtagtta tccttatccc
     3361 tggccatgcc actgctcttc ccataagaca tggttccgcc catcgtcgag acattctcgg
     3421 tgatcggcat cttcatatca atgcctaagc cataggtgta gtactcattg ctgccgaagc
     3481 tctgcttcca gttacccttt agggtcaacg tatcagctgc aagcggcatg ttcaagtccg
     3541 catagccaat gtacgaagtt gcattggctt ccacttcatg ttcccaggtg aaccctgcct
     3601 tcagagggct gaatgccaca tcgaacccaa gatcagtggt ggtggcacac gcgagctcat
     3661 ccatcgcagg gcagccgtta tggtcctgag ccaacccgcc cctgagggtg acgtcgatag
     3721 gtccaagttc catttcatac tcggcatcgg ttgcgtaagt cactacatcc ttgctggcgt
     3781 accactcatc acctgcgcga tcgatactgc cggtgatggt gagaccgttg agcgagtcag
     3841 tcttaacttc gaagcctgct ttgcgcgtgt tggcgaccac tctgtcagga tcgttgctga
     3901 ccgtgcccaa atacttcgga gcatagccat tgtcaacctg ttcgaagtag cctgtgaggt
     3961 tcaaaacccc agccttgcct gccgcatgca ggttgcgagc caagaggccc gtggccacgt
     4021 catccttgtt ccaagaagag atctctccgt caagcttcca gccttcgccg agcttcagcg
     4081 caaaccctgc gccagcaaca ctctcgttgg gcgtagtcgc ggcattcacc gcagactctt
     4141 catcatcagt tatcttgagc agcctgccat ccaccttgag gcttcccaat gtgtagctgg
     4201 ctccgccgcc cagcacatac tgcatctcat cctcagcagg ttcgatcttc gcaatcattc
     4261 ctgtagtgac aaaaggcccg gtggtcagat cgaggctgag accctctaca ccagcatccc
     4321 caaatacctt ccggctgatc tgatacttag tgaagttcct cggtctggcc aaatcgcctg
     4381 cgagcacctt ggcattgccg ctcggcgtgg tcacatcgag attgagaccc ccatcgaaga
     4441 ggccaacctc cctgtcaact ttgccaaaaa tgtcagttac agcgctgagc cctacatcaa
     4501 tgcttacgcc cttttccgga actatgttca gctcgagttc gagcttgttc tggagaactg
     4561 atgtaggatt atacttcttg ccatccgggt tccagggatc ctgatagaac ccgtcgccgc
     4621 gcaggtcgat atctgtgaac tgcgccgtgt tggttccagt aaacctgaac ctgttcaggt
     4681 catcccagat ccctgagatc ttctcatcat gctctgccgc cttgccttca agcttggcga
     4741 cgcgctcctc gagcggagct atgtcttcag tctttatcct gtgatatgtt tcggcaatat
     4801 ccttcgcgtt ctggtcagcc ataccgcgga ctacagccaa ttcccgctcc accgcgtcta
     4861 cgcgaaggtt aagagcagcc agttcccggc tgaactcagc ctgcagagca tcgatggttg
     4921 ccacaacggc gtcgaccgct gccagacact cttctcgagc cgcaaagacc gaatcatagg
     4981 tctccatggc gacttcggca tggagctcca tgagcttgag atcaaaatcc tctgccaact
     5041 catcaatggc tcttgctctc tcatcggctt cagcctcaac cgaatcatag aaagccatca
     5101 tcagctcggc cctgagttca tcgagcgcga gctcaaatct ggactccgta tcctcaaaca
     5161 tggccattat gtcttctcgt tgagcttgga tggaatcata gaaggccatc atgagctcgg
     5221 cgttctgctc gtcgagagcc aactgccact cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnntagaagg ccatcatgag ctcggcgttc tgctcgtcga gagccaactg
     5401 ccactcggcc ttcaaggcct cgagatcagc cgcgttgtca ttctgtgcag cgcggatcga
     5461 atcgtagaag gccatcatga gctcggcgtt ctgctcgtcg agagccaact gccactcggc
     5521 cttcaaggcc tcgagatcag ccgcgttgtc attctgtgca gcgcggatcg aatcgtagaa
     5581 ggccatcatg agctcggcgt tctgctcgtc gagagccaac tgccactcgg cctccatatc
     5641 ggccattttg cggtcttgct catctttggc ggcctctatg gaatcataga aggccatggt
     5701 gagctcggcg ttttgctccg cgagcgccat gtccagcttt gccaggacat catctatggc
     5761 gcgatcagtt tcttctttgg aggccttcag agaatcatag aaggccatag tgagctcggc
     5821 gttatattca tccagagcca gctgccaagc accgctcaga ccctgaagcg cctgttcatc
     5881 gccttcagcc gcagctttaa gggaatcata gaaggccata gtcaaagcgg cattctgctc
     5941 atcaagagct agttggagtt tggcctccag ctctgccatc tcacgacttc cttcatcctt
     6001 agcagcttcg agcgaatcat acaacgccat catgatctcg cccttctgct cggatagggc
     6061 ggcatccatc gacgcgccta gggcatcaat cgccttctgg cgttcgtctt cctctgcatt
     6121 gacagcatcg ctaagcgcct tcataatttc ggctttctga ctatccaaag ccaatccgat
     6181 atcggcaccc tcgctcacaa ccactgtggc actacgcacc tctttaagag cctcaatagc
     6241 ttcccaaagc ttagcatgtt cagccttgtt atcctcgctg aggtcatcaa cccgcgccac
     6301 cagctcataa agcttctcct caaaattcac ccactgctca tcagacatag cagcgatctt
     6361 ctcatagatc acctgctcta agatggttgc atcgggcgac gcggaaggct gcaagacagg
     6421 ttccgcttca ggttccgctt cctgctgaac aaccccagca tccaatctgc cttcaaggtc
     6481 aaacattttc tcggacatct gcctgatgtc tttgtcgagg tattccagtg ccctagccac
     6541 tatcaaggcc acttcgtacc ttgtcgcagg ctgtgttccc ttgaatgatc catcagggta
     6601 gccctcgacg atccccaacg ctgctagcat cgtcagtgcg tcaagtgccc agtgcgactc
     6661 ctccggcaaa tcggagaagg gactcgccaa caccgggatg gcaatgatgc caatcatcgc
     6721 ggtggcaacg agaacggcca ggagtttctt cattagtcct tcccccctca aattgatcct
     6781 gatcaactag acgctaagaa ctcaatgccg atagaggcga ccaggacgag tgctcatgtt
     6841 tcctcgccct gcaaccaaaa cggggacgag cccttttgcg tacaatacgg ccaaacaacc
     6901 acagctttct cctgccgttg ttagggccga tcagaacata ggtatgacca agagtatacc
     6961 tgcaatttca tattctccaa acactggtca actccttcat gaatcagaag atttttgaat
     7021 actccaccag ccgcccacag gctaaatgcc ctgcatcacc agttcaactg ggggagactt
     7081 gtgcgaccgc catatgtctc gacgcgccac ggcctccact tttacattgg cattgctctt
     7141 tacaatctca actatcgcag aagtaaaatc ggagattgtc atgatggtcc cgatccggcc
     7201 ttcctcatcg ctggtcattc cctttccgat gcaggctgcg ttgacgtctt ccatgagcgc
     7261 caagagctgt ttttggacct ccgctccgcc ggcggcacca ttaatcgtcc tggagacgat
     7321 aacatcccct gctgagtaga tcttggcatc gggaaatacc tccacttcta caacgagcgg
     7381 atctccatac caggaattct tgtacgacag caccctaaag atcatctttc cttcagcctc
     7441 atgcaagtcc tttatggcgg caaggtagtt gccttcatcg aacagaagga caaatggatc
     7501 atcatccctg ctggaacgag cccccctctt ggaggctatc gcattcactt ttagcagaat
     7561 ctccgttacc tggttgttca cctctgtcct tggccgcgag caatccacca cttcgccgag
     7621 aatcacctct ttggctcgga aaacaacctc tccgtaccac gacgatttct ccgcttccgc
     7681 cattatgagg gcgcttgtgg ccgcctgagc tgtcatgtcg agcttcatct gctccaggct
     7741 cttttcgagg ctggcctgcg ccgcagacag agaagatacg ttccgctcca gcgacatctt
     7801 ttcgctttcg agcgacgcta tttcatcgcg cgccctttca agctcgccgc tggcgcgagc
     7861 aagatctgcg cctgcttgct tgtatctagt ctcggtctct ctcaaccggc tttcggaatc
     7921 acgcagtttg gactctactt tggcaagctc ctgcaaagcc cgcgaccgtt catgcgacac
     7981 tcgatccagt tctcgattgg ccgcttcaaa cttggattcc agagcatcgt attcgtcggc
     8041 tacctcccta actcgagcgg ataagacatc aacctcagcc ctcttggctt cgaggtcatt
     8101 gttggcagta gcgagcgaat cctggagttc acggatttcg aagagggcga ttcttacatt
     8161 ctcagaagcc agagaaagaa ccaaaagagt gcatccggca atgatcacgc cagtgatgat
     8221 ggtaatcacc atagaagtgt acttgggcct cagcccaaag attgaaaggc gttttcggcc
     8281 caccttcatg ccgatgcgat cgccaacata ggcaatcacg cctccggcta ttgttaggac
     8341 gattatcagt acgactccgc ccatcacgta tccctcccgg caagaacaag gcttagaggg
     8401 tgaaccgctc tcctaagtag aaacgccttg caaaagcgtc ctccgaaacc tcccgcgcgg
     8461 tccctgacac aatcgtgcgg ccttcatgca tgatgtaggc ccgatccaca atggccaaag
     8521 tatctcttac attgtggtca gtaatgagca ccccaaggcc tgcttccttc agattctcca
     8581 ctatgcgctg caactctcct acggctattg gatcgactcc ggtgaagggt tcgtccagca
     8641 atatgtaccg aggctctgcc gccagcgcac gcgctatctc cactcgcctg cgctctccgc
     8701 ctgacaatgt gaaccccatc tgccgacgta aaccgactag gtcgaattgc tcaagaaggc
     8761 tttccaggcg ctctgtccgc tcactggagc tccccgtgga aatctgcagc acagccgtga
     8821 tgttttgctc tacagtaagc ttccggaata tggacggctc ttgcgcaaga tagcccaacc
     8881 ccaaccgagc ccgctgccac acaggtcttc gcacaacatc cacgccgtcg atggtaacac
     8941 aacccgaatc agacgattca agcccgacca ttatatagaa ggtagtggtc tttcccgctc
     9001 catttgggcc caacaggccc acaacctcgc cacgttcgag ctccaaactg gctccgtcta
     9061 ccactcgtct tccctggaag ctctttacta ggtcggtggc ccgcacaccc aacaagaact
     9121 cactcctcat ctaagcaaat acaatcttgc caggcaccac ctattataga cgacagcaag
     9181 cacccacttg ttccaatatt cggccccaaa catgaaaaca tgcacgacta cacttgatag
     9241 tctttttcga cacagacgtc atttctacct gcacaagaaa ggttgtatcc atgacgggta
     9301 ttgctggtat gcagatgaaa gccactgcag gagccgctga aggcattcgc caaaaacatg
     9361 gacagctaac taaaagcggt ggaccgctac gaaaggcttt cttactgccc cttctcaata
     9421 gcctgaagcg tccccctgtt cgccgcgata gctgtcctga cgaccatctc cgcagctccc
     9481 gccactccca tggcttcgcg caactcccga gatattctgt cgcgacgggc agtgtcaccc
     9541 aaaagctcga cacacgggat taccacgtcc tccgcgcgca agtgtccaac aacctccggc
     9601 gctaccagct tttctgccct ggcattaggg acagacagga acctaccgga ttcctccacc
     9661 tgttccacag cgcgctttcg gaagagctcc ccaacgaacg gaagtgagca gagaaatggg
     9721 gaaacaccgg gaaaaggcac agcagtaggc acattgagcg gcaccgcagc cacataagga
     9781 atacccaaga atgccagctc cgaacagata ggaccaggag tgcatatcgc gatatcagcc
     9841 gccatcatgg cctcgtaccg cttcatagtc tctattcgta catgcactcc cccagcggta
     9901 acagcctcga ggggccctgc gctcttctgg aggatagcgc ttgagccatc tatcttgccc
     9961 ctgtgcttgg aataggagaa gatcgaatcc aaagaagcag tgccgatgaa aggagaaaga
    10021 cagaggacga atttcaagtc ccgctccacc ttggcaagga gctccactgc gcgaaataga
    10081 aacggggcca ttaccgcagc ctcctgaacc ctagttccag ggaacagcag cactaacctc
    10141 tcgccgttcg caacccccag atacgccctt gcctggtcac ggtccattcc ggcgcgaacg
    10201 ccgtccacca tgaggtttcc gataagtctg acgcgctcgg cggggacacc tttcgcaatc
    10261 agcgaggcct gaatcctctc gtcctctaca agaaatgcag aaacagtctt ggcgcccaag
    10321 atcgtcctgt ctgtatacac aacaagcggg taacgcaatc gtcggcttag gagaaccgag
    10381 caccaaagat tcccgcccaa atgaatcaca atgcccgatc ccgaactggc aaaacccgat
    10441 ggtctcttgc caaatgccag aaaggcaagg aactcgcgga cagacaccgc cctcctcact
    10501 cctgggtagg tagaagcgac tttagcctct cgtccactgg catagcctcc cggggagaca
    10561 aacatccata tatcagccgc cggcagcatt gacagagcct tttcagcgac gggcctcgca
    10621 agcccatgaa cttcctctgg agagctggag accagcacta tgtccaaatt taaccacccc
    10681 acttgcagca gaaatgctgc tcgtcaagtg tctactgagc atatcgggtt tctacaggcc
    10741 cacctccttc tcctcccgat ggcctgcgaa atatgacaaa gactccgaat actgacgggc
    10801 caaatccgag cccggcgtga gcaacacatc ctcaggttta ccttcgtcca cgaccgctcc
    10861 atcggacacg agcacaactt tgtccgcaac gcgcagggcg aactggacct cgtgagtgac
    10921 tattatcatg gtggatccga gcgaacgggc aagtgactct atcagcttga gcacgtccac
    10981 cacgaggata gggtcgagcg aggccgtagg ctcatcccaa agcaggagtt caggctcttg
    11041 ggcaatggcg cgagctatcg ctgccctttg catctcaccg cctgacagat tcggtggata
    11101 tgctgccgcc agacgctcaa tccccacctt ctccaaagcg ccgtatgcaa gccgtttagc
    11161 ctcatcaatg gagacccctg ccattacacc gggcagcatg gcattttcga ggacgctcag
    11221 gcgcgacacg agattcgaac gctggaacac aaagcctgtc ctgcgtctgt acgatctcag
    11281 ctcatcagaa gacataccca aaaccgaccg gccccgccac agtattgacc cggagtcggg
    11341 ctctgtcagc ctgataatgc accgtagggc tgtagacttg ccgcatcccg acggccccat
    11401 gataactaca gtctcgccct cgtgtacgtc gagatcgaac cctgaaagcg cctggcgcga
    11461 tccgtacgcc tttttcacat cgcgcattgc gagcacctag ttgaacctcc ttcccgacga
    11521 tcttttctat cggcagacac atgatcaatg ccgtcgaaga ccacgtgagg ccggttgatg
    11581 aaggacagaa tcccgggctt tccggagggt ataattatct ccctcatgat ctgcgttccc
    11641 gtcataccaa acgcaataga ggcctcgacc tcatcgaggt cgaccggcga cattttgagc
    11701 gaaactccgg cgcccacaag tcccaagagg cctccgatcg cggcaaatag cgggccccct
    11761 gcagacccca gcgttgaccc ggtaattgcg ccaatagcat aaaggctgat cgctcccgcc
    11821 aaagccccgt aaagatactc ctcaaaaccc ggacgcagca cgccctgcct tctcctttcg
    11881 agaccgatag cgcccgcaat cacagtagaa tggtccacca tgagcgagac agcaacgatt
    11941 ccgcaggcag ccaacccgga tatggtgctc tttactgacc tcagcaggtt ctgaacctca
    12001 ctgcgcaccc ctggttgaac tatccccaca ggtatagtgt aaactgccag cacatccttc
    12061 ccggccacct cctcgacggc tgttgctagc tcatcaggag tcagccccga ctcaatcagc
    12121 agttgaacct tgtcggaaga gccctgttga tccccgacaa gcttgttgat ggccccgata
    12181 gaactgacga tctcatcccc atcgaacttg ggaagcgcgg atttcacctc ccttacctgc
    12241 tctgcaatgc cgtccacatc cacctccgac atctgagcaa gctttacttt cgcagcttcg
    12301 atgttctcgc gcaaagaggc aggatccagc tgatgcaaag tagataggat tcctgacgcc
    12361 gcagacgaca tatctctgag catctccgcc acctggcccg cgccggttgc agccgactgt
    12421 atcgtctcca gtttctgagc gaacgactcc agcctggcct gccacagctc gagatcggca
    12481 aggagcgagt cataacttcc tgagaacatt acaacaacgt cagccgtgga gcgcagagct
    12541 tctatcgcct ccaacgcctc ttcagtcaga tggagcagcg cgctcaccgc ctcatcgtcg
    12601 atgggcatga tgtccgcttc gccatctacg ccaatgctct ccagggccct ttggagataa
    12661 atgagagagt ctatggcccg ctcgtatgat tccaaagctt cctctccaat cttcgcggcc
    12721 ctcatagcat ccgcaatccc tgcatcgtac tgtgccagtg cctcttccgc ctccgatagt
    12781 gaacgggaca ggtcctcctg gggccgactt gtagtagcgc ttcccacacg gcctgaaaca
    12841 acaccgtctt ccagcagata tgctgaaaga acctgcgacg actggtcttt gggctctttt
    12901 gccccatcga ctattattcc gcgagcctta tctccgtcga catcagtcac agacaccacc
    12961 atatcttcag gcgccggcaa cgatccaacg ggcttatcct ccacagacaa gacaacagaa
    13021 tctcctgtat ccaggcctga aaaccccgta tccgccccag tgtgcacagg gttgaccttt
    13081 ccctcgtcca gtcgtataga agcttgcggc gcccaggcct ccaggaatgc tttcatcttc
    13141 accatagccc cagccaacac ctgggcctcg tcggcctgtc cccgcggcgt cacgtcgcgc
    13201 acaacctggc ccttcacatc cccgcctgcc aacccggcag ctacgcgttg cacaagtcgg
    13261 ccccggaaag caccttccga agccagtgcg ctcggcgcaa acctaatatc gaccacctgg
    13321 tagcgcccga ggattgcctc ggccgcctgc gccacagatt tcacactctc cggcgactca
    13381 catatgagat gaatatctcc gccgtcgcgc aaagcaaact ccactccatc cagccgcgtc
    13441 aacttatcct gaacaatcgg cgctgtcttc gaaccgacac cactcacggt caccttaggc
    13501 tcgagcaaga acgtataccc ggcaaacccc ggaatgccgg acaccccctg caccaaagct
    13561 gtcactgatt cctcgtcctt tttcgccaac gacacaagga agttcgacac cgacaagacg
    13621 gtggcgctct ctttgactcg ggcccccggg agcttcgcgc gcagtgtcgc cctgagcgcg
    13681 cgcaaagcct cgcgtctgga ctcagtcctc acgtggacaa tcagatcgta ttcgccgtat
    13741 tcgcctacta catctgatac tgtatgaaca aagaaatcat cggcagcgga cgcaattcct
    13801 gccgctacaa acgatcctag tatgattgtg actagaagca ccgcggcaac gtccagtatg
    13861 aacccaccgc gcacaagggc gtacatgcag ctaccccccc ccattcactc gacattatag
    13921 catgtgtgcc cactttgcac ctctcttttg ccatgtaatg tcttccatgt ggccgcaaat
    13981 gacatagcca gctgaactac gggcactgaa agctccagtg aatcccgctt caggggcgtt
    14041 catcagtaac ctgtggattc tacagcagct tttggcgagc tcgataggga taatcgggag
    14101 catcaccctc tcacagaacc gtgcgtaccg acctcgtaca cggctctact tttagacctc
    14161 actccctaaa agcagtccca ccaacctccc aggttatcca gctgacaagc caatcccggt
    14221 tcgatttcgc tatgttcaca cgtaaacatc tttgggtacg ggcaatccag gacttctttg
    14281 atcaacaaat atgccgggcg aaaacgcggg aaggagggcc gctttgcggc cctccttcat
    14341 acccgatctt cactcgacta ctgactacac cttcgcatgg aaagggacga ttagcgtcac
    14401 gccttccatt cttcttttgg tcccctaaaa aacccagcat cgatcttggt caggaacaac
    14461 gaccagccac atgcgttact cctcgatctc aaaggtgatc tctacaggct tgccttccgc
    14521 ttccaccagt tccttatcag tatagaggat cgccctgtca cattttatgg tcttgttccc
    14581 ctcgtcctcg atggcaacag aaccctgcaa agtcagagtt ttgccggcgt tatcgtaatt
    14641 cgccttttgc gcctgggctt tctgcttatc cttagtcatc aagaccgatc cctgggcgat
    14701 aaaatcctga gtcttggttg agatctccag aatgttgcat gtcatcttca caacagagac
    14761 cttcgccttt ccggactcgt ccttttccgc ctgttcctcg cgtttttcca gcctcacatt
    14821 tcccttcact acagcagtat cattcttgag atccacatcc atctcatcag cattgagtac
    14881 aacatccttc tgcgttaaaa gaacgccgcc agtcagtttg gcagtctcag ccgcattgtc
    14941 tataaccgcg ttcgtcgcag taatcacagt gtcgtcatac cgtatggtca ggccgccctc
    15001 tacaatagtt ctgcccttgg aaccctcata acgtaccttg ccatgaactt gcaggttcac
    15061 ctttttcgcg gcggcctccg caacaatggc cgctgaaacc aaaagcaccg ccaaaaccac
    15121 aagagacatc gctcttcttc gcgcctgccc cttcattccg ctctccccct caatcgtcgt
    15181 cgccaatctc aaactcaatc tcagtcccgc cccatgcttc gaaggagttc tccgcagggt
    15241 cgaaacaaat ctctccgctt tttacgacaa cgtctcgacc accgaacccc tcgcgaatcg
    15301 tcaagtcacc gctggcataa atcttgtcgg aggccacgtc cacccgaaga gaagcagcag
    15361 agaccacccc ctgttcggta attatctctg cctgccctgg aatgacaatc ctagagtcat
    15421 cgggcttcca tgccacttca tccgttctca acagcttccg ccctcctgag aaaacatcga
    15481 cacctcctga gagcgacagc tggcccgtct ttgtatcaaa aacgcctttg catgctctga
    15541 atgagacata gggttcgcca tccctgtata tctcgccgtg cccaatctcg ctcatctcca
    15601 catggccgtc gccatctcgc agatccacat tggccgcgga aatcttccac accctctcgc
    15661 cgttttcccg accctcgata ctgacgccgc caaacgtgag atcactggag gcgcggtccg
    15721 gatccgggtc aaaacttcga tcttcatcgg ccggcttgtt gagcacgaaa gcgtaaacgt
    15781 acaggcaacc aagaaccagc aatatggcaa cactgactat cctgacaatc ctcgggtcca
    15841 tcagcatcac gcctcctgac aagccctcag cacggccaaa ggttacaccg gagtgttcat
    15901 gtcgcaacct cactgttgaa agagtattcc ttcttcgccc aggccaaacc cgagaggtgt
    15961 gcctgggaat gccttcagcc tgaactcgag ccacacccgt ctcgacgtgt gctggtatgt
    16021 gagagccact tccctgcagt gcagatcgcg ggtgatccct atgtctcctc gcacgagacc
    16081 gcccttgaag aaatcataca caccagtcca ctgcagacgc cattcgggtc tgaacctgac
    16141 atctcccgac gactcaaccc tggtaatctg tccgcgaaca aaatcataac tgccgccggc
    16201 ctttacctgg atatcaggcg acggctgggc cacgactttg cccacaagtg acgtcggatt
    16261 cccgctctcg agatcgtatc ttcccgaaaa atcgatcgac agccctttag cgatatccag
    16321 ccgcaaagcg gcgttcaaag gatcatacct ttcggagaca acatcatatc ccgtcgataa
    16381 agtgagcttc gccggtccta gcgatgtcgt aaggttcccg cccagctttg acccggcatt
    16441 gatttcgtca aacctgaagg gcgacgaacc atagataccc gtttcggtat aggtgagcct
    16501 cagggtagtg ccggaaaacg gctttacata cgccccgacg gaacatacag cggccatccg
    16561 gtctccatag ccctgataga catcgaagac tccacgtgca tatccgtcca ttgatagcca
    16621 tggaaaaagc tgacgagact tggtcctgag cgtcagatcc agctccccct tggccgcact
    16681 cacttctgat cgtgtcccgc tccgaattgc atcctcaacg taatttccaa acacgccctt
    16741 cacatcaagg atgagagaac tctcgccgac agcaagtccg ctggctgtag ccgtaaactc
    16801 cggaagtttc ttcagagcct tccacggagg cggcttagtc tctacgtcac tcggatcttc
    16861 cacgtatacc tgggcctgcg caagccccat caccttgaag tccccgtagg cgcggtcagc
    16921 ttgcaccttg tagcttacta cgtcctgggt gacgtctgtg tcctttacct gcttcttata
    16981 gtctacattt gaagaaagct ttatgtcatg gagcaaagtt gaagagtgcc tgaaggttgc
    17041 attagcgact gaagcgtaag tggctccgtc agtctccctg aacgtgccgg caaaggaggt
    17101 gcttgagtcc tcgactcgcc tattcagact gagattgtag gcacgctcag caatttcatc
    17161 cccgagaata ttgacgtagt ttctctgttt gtaaccggcc tttgcaccta tatcaaatgg
    17221 cagcttcagg tcctgagaaa gttcgagcgt agtgtcgaag gtaccggaac ctgtgttcca
    17281 caacccgtaa aggtaggccg tgagcacgcc gcccttacct aactgcaagt tttgagttat
    17341 gccgactgcg gggcccaaca gctggaagta gtcggcatat aaggatccgt aagcgtcttc
    17401 actcagcttg tagttgtact tggtcttcaa gtaccagccc tcatacgcac cgtatcccac
    17461 cctgggaagc tcgaaccgct cgtcttcctt taggggcacc acgagtcgcc gcaaggtcag
    17521 caacttcaga tccccctcgt aataggagac agacttcagg actatcctgt catcaacata
    17581 gacctccatc tcccctactt ccaaacggta gtggggcttg tcaagatcgc aagtagtaac
    17641 agagccatgg gaaagccaaa gataatcatc ttccttttgc aactggcttc cagacatgta
    17701 caggaatcct tcaacgcctt cgcctgtcag cttagcccag gcctcatcaa ccacaacctc
    17761 gcccgtggaa acgttgaaaa tggcgctttc gcccgcgagc gcatcctgcc ctgaaacgac
    17821 cctgaccgac ccgtttactg ccacaacttt ccgatccata ttgaactcag cgtaatctcc
    17881 ggatatcacg agatccttgt actcgatctt cacattcccc gacatcaccc aaagacctat
    17941 tgcgccgtcg tacacaagtt tttcggcgtc taccttgaca ggaatattgc ttgcgaccgc
    18001 aggaggggag cagattgcga aaatgtacag agtcgtcaag atcgataccc caagccacat
    18061 atgaacgccg cctctggaaa acttgatccc agcagaccca tcgccccctc ccgaattcct
    18121 ccttcttgca gccaaattca ttcggggacg gtcaactgcg aacaacatga aaagacctgg
    18181 cactccaaag acaatattgg gcaaccaagc agctaaaagc ggggacaggc cgcccctcat
    18241 ggccacagat cgcgccagag cctgcacgat gtaataggca aaggagagaa cggctgccgc
    18301 cacgaccccg aagtaaccgc cgccacgtcg gggagacttc acgacaagcg gggcaccgag
    18361 aacggcaaag accaaagatg ccacatgatg ggcaaccttg agatagtaat cgacgcggag
    18421 cgcatctaca cggatcccgc tcctctcaaa aaggtcgata ttggacttga gttccttcat
    18481 gctcatttcc tctggggtct tctggctcga tgcgaagcta tcgagcgact gctccaggga
    18541 aatctccaga gtctcaaagg aaatcgccat tcgcacatgc ccttcctcat cgagctcatg
    18601 gatactgcca tcctcaagca cccacttgcc tgactcagcc cgaccactcc tggcagagac
    18661 aaggcgcgga aactcgcccc tagccgtctc gtatatcatc acacgttcaa acgtgccacg
    18721 tttttggtca gccttgccca cgtagaaata gcgattgccc ggcgccttga agaaaacatc
    18781 ctcttcgaca tcgggaaaag cctcctgaaa tatcatctcc ctgacgataa tctcggactg
    18841 atgagttgcc cacggcgcca cgctctcatt gagccaaaat ccgactgcgc tcataaccgc
    18901 ccccaaaacg atgagaggca aagcaatcct ggcgaaagga atcccggaaa cccgcataat
    18961 ctgaagttca gaatcgcgag ccagcctgcc aaggccgatc aagacgccaa acagaaccgc
    19021 aacaggcaaa acctgaacca cgatccccgg aaggctgtaa aggagcatcc tagcaacaat
    19081 gagcacaggc actttcttct ggactatcag gtctacaagc cagaagagtt gcccactcag
    19141 cattatcacg atgaacgcca atatagatag aataaaggga cccatgaact cgtggactac
    19201 atacctgtca agaatcctca tcagtcgtga cctcgcagtc tacagctgcc tcttgagcgc
    19261 aactgagcat cgcaatcgca tctttggcac aaccccatgc atttcaacat aacagccctg
    19321 cccccgcggt gggcatagag cgtgccaaag cgcctcaaga caagaagggg gagacgaatg
    19381 tcgcctcccc aagtgtgatg cactgccgct acttgctatt atttcgcccc gccttttttg
    19441 aggacggcgt ctgtcatgtc tattccacca aggtggacaa cgctcgagtc cagaacgacg
    19501 gtaacgccct tctccttggc gactgccgct atggcgctct tcagagcatt ctccgcaggt
    19561 tccagctgct gctttgcata ctcattgtac ttcagattat actggtcaag caaagtcctc
    19621 tgggtctcct gatcctgccc ctcagccttg gtgttcagtt ccttttccaa ggcagccttc
    19681 tgcttgagaa tgttgtcaac agctttctgc atcgcaggga attcctgtcc aagcctgacc
    19741 ccatcaactt ttcctatgac agcggcctcg ccagcggccc gcgatacagc cgaaagatta
    19801 gccacaacag caacagcacc gaccaaaatg gcacacacca aaacaatcag gagagtcttc
    19861 ggcacgtttt tgaacattaa tgtcatcctt tccaatcaac tcaaaaatcg caatcagaac
    19921 agtataacga ggagcgccga cgacgcggcg ccccgtcaat tatactacat gagccagctt
    19981 aagactagaa agtctgccca aagctgaagt aggcctgtcc tcccttttca ccgatgccat
    20041 aatcgacacg gatcatgccc agcaccggaa ccatgattct cacgccagct ccataaccga
    20101 ccttagtact tccaacgtct aaagagccat ccgcgttcca cgcctgcccc gcatcgcaaa
    20161 acagcacacc ctgcagacct ttggtaatgt tgaaacggta ttccgcgttt gccagggcca
    20221 tgcgatcacc ctcgatgtcg ccgtacttgt agcccctgac agtctccgct cccccgagcc
    20281 tgaaacgctc atcatcaggc agctcgtcca atccatatcc ggcgctaacc ctacctgcca
    20341 gaatatgccc atcacggacc tgatagtact ggcaagcatc aacccgaagt ttggcaaagt
    20401 tgttgtcgcc gccaagaatt ccgcctgcat actccagcga actggacaac ttgtgacctg
    20461 tggcaggatt ccaaaggtca tctctggtat cattgaccgc cgacaacgta agcgaacgcg
    20521 tagcaccgcc gcccgggaaa tccacatcag gatgcttctg ttcagggtca ttaacagcgt
    20581 tttccatctt gagcttgacg aacgccttag tgttgagcga aagcgtgcgg ccgatcgtca
    20641 cgtctccacc cgtccgcttc tgcgaataat cgatagaggc cgtcgggtcc tcctcgagct
    20701 ttaagacttg ccctgaggaa gaagtctcgt tttcatcaga tggcgtatac ttcttcgaca
    20761 aagtcttgct gaagacgttg aagcctatgg aagtcttgcc ggatgctccg acccaaggtt
    20821 catagaagcc aagttcataa ttggttttcg tgcggccaaa ctccgctttg atgttgaccg
    20881 tctgcgcatt gccgaggaag ttctcatctc ccagctccat ataacccata agcttgtcct
    20941 gagaactgta tgtcagggcc agattggcaa tgcccgtctt ccgttcagtt aggacgtacg
    21001 ttaccacgta ttcatacttc cccggcacag gctctacttt cgcctctaca ttctcgaacg
    21061 cgccaagcat gtagattcgc ctgcggtcct cgtttatctt attgacattg atgtagtcac
    21121 ccggctttgt ctcaatgcac ctttggataa ctttcggctt agttttctga ttccccgcaa
    21181 acttgacttc cgacacagtc cattccacca gatgaagtat gagttcaccc gattcagaaa
    21241 gtgatggttc aatgctcacc cagtagcctt tgtcatggta gtggctgccc aaagcctcca
    21301 ggtcactcct gagctgattg gcattgagct gagagccaac cttcgacccc attattccga
    21361 gaagctcctc agtaggtatt agtgcgtttc cgctaactag aatcttgctc aaaaccggaa
    21421 attcttgcac tacaatgatg agcttcacgc ctccaaggta cggaacggtg ttggcgccga
    21481 gatccgcaaa ccagccgaga gcatcgatag cggtgaagct ttcccttatg gcgctttcag
    21541 tgagctcatc acccacctta atgtttatgg cctgtgtaat aatatcagag tcaatcctgc
    21601 tattgccaat aacttctata gcggtgacca ccccaagaga cttggattgc gcagccatcg
    21661 taaaagaaga tgccaaaaac acgaccgcca ttgcggcagc cagtatgcaa caggatcgct
    21721 tggtgctagt atctttcaaa aatctcgcct ccccaaatcg cgctgttcgg ccacgactgc
    21781 agcccgctca ggccaccatc ataaactgat gagttggatt cagtattcta ccctcgccta
    21841 gcgattcctc ccgcctcaaa gcccgggaat ggcaatcaat cccggctcgc ctgaggcagc
    21901 gcgcccacga gttggccaag ccggctcaga atatacagac gcaaccgggc gtccagaagt
    21961 ttcatgagaa gctcccccga cggaaagttt tcttccacgc ccgacgctgg ctcgaaagac
    22021 aggtgaacat cgccgggcct tccatatatc gtggccagta tgggaaactc agcagaggaa
    22081 tcacgcgcaa cgacagaaaa atcgatatct gaatctggag tgttcgcaaa ttccaccctc
    22141 ccggacagaa tctccagagg aatatcgcag atatacacct ccccgggtcc cacttgtaca
    22201 taaccgacag cccggggttg cctcacactt ccctggatct gcactacaga gggcatgatc
    22261 tcagcccagg attcttccct tacggccttc actccatcac caacaacgat ctttatgtca
    22321 aggtcggcat cgggaatcac agctgccttg gaaaatccag cagtaaccac atcaacatcc
    22381 gcgttgcaga gactgagaat gcccccgact ttcatccgct ctccggtgtg gattgtggcg
    22441 tctatgtcga ctaacccgca caacagcgga tgctcgattt ccaaattcaa gccatgtatg
    22501 gtccacatct gtcctccacc ggagagctcg actgttccgc ctcccattgc agctcgaatc
    22561 gtgcgaaccc tgacctcttc cagcgaacct gcaccattat tcggtatagc ctcagcaacc
    22621 tctatcgaac cgcttaggtc cgtgaactcc tgaggccata gttccgatct gagatcgcca
    22681 tcacgaatag tcaccacagc ggccagcgtc gaatcttcaa attcgatatc ggctttccaa
    22741 tcaacccatc cttcaactgg aatagcacgc cccaaagcct tgccaaccct ctccattgac
    22801 atgcgttccc cgcctatagc ggctttgatt ttccctccgc catagcttgc atctaagtct
    22861 aggtctatat cgctggcccg atgctctccg acactcaagt cgaccaggtg gaggactcca
    22921 tgggcttcgt atgcactgcc tgcctcggca gcaggccgcg catcgctcct agcttcattc
    22981 cttgttccta tctccactcc cggtgcgcgc aggcagcctt cgtattctgc ttcaagaaac
    23041 ccatctattc cagcttgcgg aagcttcaca ctcccacgag ccgcaacagc tccatcttcg
    23101 tccattctaa cataaccgga gattgtcgac tccccgcaac acagatccgc tatgtcaaga
    23161 cttccagtga gaaacaaagg gccatctacg cttggacaca ggaccaatct tccccttaca
    23221 ctgcctccta ggagttgcgc atcgatgtcg gaaagctcca ccgtctctcc gcggatatca
    23281 acaaaggcct ttgccccctc aatatcaaac gccgtaccat cagtgtacgt ggcatttgaa
    23341 agcgcgacct tccagacacc gcacccatcc agcaatgtgc tctccacggt caccttcgca
    23401 acctcgagct tgcggaaacc ctggtcttct gatggaaact caatcagaac atcacagatc
    23461 tcgatgggag cgctgaaccg actgagttcc gtcttcgtcc cgccatcaga acgcgcaccg
    23521 ctctttctct ccacatggcc gcggtttgca ggttcatgga ctaacggcgc tgcgatgtcg
    23581 acgctgacgc cgcgagcgtg cacaccggta atgctctcta ttaacccggc ccgaccgaaa
    23641 aagagatcag atacgtcgta gtcaacaaga agatggtcga tactgacctc gtcacctatg
    23701 caaacacctg caaggtctat ggaacggatg cccctcgccg tcacgtgccc tatccgaaca
    23761 tctgagaggc ccaacacgcg tgagatgccg aggctgaccg cctgggacgc catgccggcc
    23821 aaattctgcc tgacccacat cgttccggaa acgatcacgc cgatcactgt tatggcaatt
    23881 agaacctttt gcttcacatt taccaccaac gacgatttcg acaaaggact catccttccc
    23941 ttcgactcat aggcagtgaa agtccggggc acaatgcagg gttttctctt attcccaacc
    24001 tcaacccgcc caggggtttc cccatcagtt cacatcttgt ctgtcataca ttacacataa
    24061 tggagggcag ccccggtcgg gcgcgctact cggcgaagat tgaaaacaca tttgatcgct
    24121 atgttcaccc gttcaaggaa gcaacaaacc gcgtcggcct tcaacaaacg ttgctcatct
    24181 gcaggtggcg tcttccaggc aaccaaatag tcatgtgtca cgactttttt gattggcgaa
    24241 cgggacttac gcagcataaa caagcacaag gggtcagcag cattccgcaa gaagcaggga
    24301 gctaggaggt tgcaggctgc atgcgtccgc aaactcagag gccgacttgg aggccgcccc
    24361 attcatgggc attggagttt tgcccggcta gtctgagaag aag
//
LOCUS       DAOSYL010000038        24141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1725_length_24141_cov_8.094578, whole genome shotgun sequence.
ACCESSION   DAOSYL010000038 DAOSYL010000000
VERSION     DAOSYL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1725_length_24141_cov_8.094578"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            608..1570
                     /locus_tag="PLU15_07235"
     CDS             608..1570
                     /locus_tag="PLU15_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014277157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOO30753.1"
                     /translation="MTNHVLAGVMGLCVGDALGVPVEFTHRKTLKENPVVGMRAYGTY
                     NQPAGTWSDDTSMTLCLADSLIRGLDYRDIMERFLAWLNEGAYTPYGKAFDAGNTTKK
                     ALYKFSRGATPLECGGTDERDNGNGSLMRILPILFHLRSVYGPGFYDIDEAFDIIHNI
                     SALTHAHERSQIACGIYVSIASALMEGKDLKTHVETGIRRAMQYYIGQARFSHELHHY
                     ERLAHEGFNRLDEADIRSSGYVVDTLEAAVWCLLNTGSFEECVLKAVNLGEDTDTVAA
                     VAGGLAGLYYGYESIPGQWLSVIARREYVETLCNELYESFRNFL"
     gene            complement(1844..3097)
                     /locus_tag="PLU15_07240"
     CDS             complement(1844..3097)
                     /locus_tag="PLU15_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOO30754.1"
                     /translation="MINRAGLQQPEVEGWNVTGPVVKLGVTKLLEEPEKYLGTARVGL
                     VANYNAVDEYATPIIRLFAESPKIEFTRIYVAEHGLWGCEQAGVKVGHVGDLRTGVDV
                     VSLYGETVKPTPQMLEDIDVLVCDGNDIGSRYWTCLSTTALCIEAAAEAGKQIIVTDR
                     PNPIGGMAVEGNLLDIEYRSFVGYDRIPIRHGMTPGELAAMYVGGAGLDVDLLVIPMD
                     NWGREMLFPETGHFWTGTPNMPGFETALVYPGTCLFEGTTLSEGRGTTKPFRLIGAPW
                     LDAIDLADRLNTMELGGVRFRPAHFRPMFSKHSGVECAGVEVYVTDAWELDPVRLGVS
                     MLIAAREQNLDAFGWYGAGSPTGSADLARPFPVEGRHLFIDLLAGGAELRRWIEEGAT
                     SDEIMARWERETSEFMDAREPYLIY"
     gene            complement(3213..4103)
                     /locus_tag="PLU15_07245"
     CDS             complement(3213..4103)
                     /locus_tag="PLU15_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOO30755.1"
                     /translation="MTNDRDENRVQQTKNADYCLVTIRGRYPSLAPAEQKVAFFILEH
                     PESAAEMTSVQLAAAAGVSESTVVKCCQRLGFTGFSQFKLALVRELATSREGAFGEVE
                     PGDDVPAICDKIFTATLQAVEDTAKVLDPSEVARAAEAIHRARRLFFFGVGSSGVVAQ
                     DAQLKFMRIGMTAFSYLDTHAQLTQAALLNPEDVAVVITYSGRTREAMDVIDLARECG
                     ACTVCITNFPTSPAAVGSDIVLCTTAHEATGLRGGAMTSRLTQLAVIDCLFTAVAMKE
                     SDEAMELLMKTVDALASRKI"
     gene            complement(4139..5335)
                     /locus_tag="PLU15_07250"
     CDS             complement(4139..5335)
                     /locus_tag="PLU15_07250"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOO30756.1"
                     /translation="MCSTMKVVGLMSGTSADGIDAALVEIEDRDSGLKAETLAFGNYH
                     YPERVREKIFELFSPKTSSADRICRFNFVLGRLFADAAVSVIEQAGLELGDVDLVAMH
                     GQTVCHLPWAGEGEFQVPSTLQIGEPAVVAERTGITVISNFRSRDIAAGGQGAPLVPY
                     ADYILFRHEDKTRVVQNIGGIANMTVLPRGARPQDVIAFDNGPGNMVIDAVVSEVTGG
                     CMDYDVDGAIAARGRVVEEFVAELMDHPFVRIEPPKSTGREEFGAEYVKSVMARARAL
                     GIGDEDIVASVTAFTAECIGKHLSDLVVPEYGLDELIIGGGGAYNPTLMRMIRERVDP
                     VPVVTHETYGISSDAKEAIAFAILGYLGIHGIPANLPSATGAHHQVLLGAITPGRRSE
                     KVWDRL"
     gene            complement(5355..6998)
                     /gene="nagZ"
                     /locus_tag="PLU15_07255"
     CDS             complement(5355..6998)
                     /gene="nagZ"
                     /locus_tag="PLU15_07255"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOO30757.1"
                     /translation="MELLDSMNIEEKIGQMVMVGLDGPELTSSDQEFIDKYKIGNVVF
                     LGRNIIDPKQAQSLTYGLQETASRRRHPIGFLLSIDQEGGVVARLTRGGTVFPGNMAL
                     GATRSEEYARRAAKVTADEMLALGFNMNLAPVIDVNSNPNNPGIGVRSFGEDVDMVTR
                     LGVATIEAYQASGVVATAKHFPGKGDVTVDSHLDLPTVPHNRERLERVEFAPFAASIR
                     AGVGAVMTAHVFFPAVEAEPDLPATLSRKVLTGLLREELGFGGVLLTDDLFMGAIAKR
                     FSIDEAAVRALEAGADIALLCHNQAEQAVAIEGIWDAVRSGRISEERVDESVRRILGL
                     KERFGLLAPERYLVRRSLKPVGSPENRRVALEIARDSVTLVANRQALIPMTPEDYPGL
                     VVMSPDIRGLTQVEDGQLAESPLVAAIKRFVPGVRDFGFSQSPDEREIAEAVSFASAK
                     DAVIIGTYNAHLYREQGRLVRALLEALDPNIPVVVVALRNPYDIQQFPSAETYIAAYG
                     FRECTMRAVAELIFGAIAPKGRLPVSIPGICACGAGLTL"
     gene            complement(7041..8084)
                     /locus_tag="PLU15_07260"
     CDS             complement(7041..8084)
                     /locus_tag="PLU15_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOO30758.1"
                     /translation="MPAERFPPLLGVGHGGEAGLLLGVDGGGTRTDCLLATLDGVVIG
                     RGSSGPSNLQAVGIDAAVCEIEKAVRLAMREAACSGGTARITSACFGLAGAGRKRDRD
                     MVEMRLRQLLAEVCSPDAPFPALTVVNDGIIALAGANAGEPGVVVIAGTGSLAYGVSA
                     DGREARAGGWGYILGDEGSAYDIGVKGLREALRAADGRQIPTCLTEDLAVALEIEEIE
                     DVVSVVYSGMTRPEIAALAPVVAKAAEKGDHVAQDIVVDAGRELGLMALAVIDRLGMS
                     GGAGNVAAVGGVLESNEMALDAMRGRVKVSAPAWSVERGKFSPAVGALLVAGRAIGGG
                     SIDEDHAPDNVCS"
     gene            complement(8091..8999)
                     /gene="murQ"
                     /locus_tag="PLU15_07265"
     CDS             complement(8091..8999)
                     /gene="murQ"
                     /locus_tag="PLU15_07265"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOO30759.1"
                     /translation="MDYADLATEETNPRSRGLDRMSTVQILEVMNEEDRRVPEVVARA
                     IPEIAKAVDMIVARMAAGGRLLYVGAGTSGRLGILDAVECVPTFGVDPNQVSAMIAGG
                     PGAVFHSVEAAEDDESSGVRDVAESITELDVVVGISASGVTPYVQGALKAAKEVGAGT
                     VAIVCNHADKLDLDVDVVISLPVGAEVLTGSTRLKAGTAQKLVLNMISTATMIKLGKV
                     YDNLMVDMQATNRKLKRRAVRIVSIATGLDEEVSEDLLDKAGGNIKTAIVMALASVNR
                     REAERALEKACGHTRNAVQAAIERTR"
     gene            complement(9068..10081)
                     /locus_tag="PLU15_07270"
     CDS             complement(9068..10081)
                     /locus_tag="PLU15_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO30760.1"
                     /translation="MKILSTTGLKKYFPIRSGVFLQVTGYVRALESTDFEINEGETIG
                     IVGESGCGKSTLGRTLVKIYEPTAGTITYYDPLGASHDISKGLGRDVRQTFRKDVQMI
                     FQNPFDSLNPRMTIRDIIREPIETHKLFDDVAAMDERVAELLTRVGLYPEYAQRYPHE
                     FSGGQRQRIAVARSIAVNPRLLICDEPTSALDVSVQSQIINLLKEIQRETGMSLIFIS
                     HNLDVVHHMSDRIMVMYLGNVVESAPASELFDEPAHPYTSALMSAIPSWNPKDRKLAK
                     VKLEGEPPSPINPPSGCPFHPRCPHVMELCHEIKPEPQEVNDGHLCACHLHARADAEV
                     AAR"
     gene            complement(10078..11067)
                     /locus_tag="PLU15_07275"
     CDS             complement(10078..11067)
                     /locus_tag="PLU15_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30761.1"
                     /translation="MTTACERILTVSNLKTYFRTPDGIVRAVDDVSFHLNRGEVLALV
                     GESGCGKSVTAHSIMGLVKAPPAKLEGSIVFDGRELIGLSASEYRKIRGMQISMVFQE
                     PMSSFDPLYTVGEQLMEVAQAHMPWGEEEAKERIIETLRLVHIPEPDKRYSEYPHQMS
                     GGMLQRIMIAMALITSPKIIIADEPTTALDVTIQAQVLNLMQDIQSEYDGSIIFITHD
                     LGTVAEIADRVHVMYAGKIVEKASVVELFDNPLHPYTAGLLASRVRREYKGQDLPYIE
                     GYVPRADQFPEGCRFNPRCLRATAKCRELQPPDLEPAPGHTVACWLYEGGDGR"
     gene            complement(11064..12155)
                     /locus_tag="PLU15_07280"
     CDS             complement(11064..12155)
                     /locus_tag="PLU15_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30762.1"
                     /translation="MEGTRKRIADSFFKHKLGMVGLVVLIIFYLVVIFADFVAPYNFA
                     ETHRNFIYAPPSKVKWVDSSGKWVGPHIHEMVKSRDPVTYELVYAEDPDSVVPLKFFV
                     RGEKYRFLGLWESDLHLFGVEASPEKAMFLVFGADRFGRDLFSRIVIGGRVSMTVGLL
                     GTFLSVFIGAIVGALSGYYGGWVDVLIQRFTELLRSFPRIPLWLALAVIIPPSWPSTW
                     VYFGIVTVLSLIGWMGVARVMRGMTLSLREKEYVLAAKVMGVSDWKIITKHLIPNTLS
                     YLIVVSTLSIPGMILGESTISFLGLGIKEPMTSWGLLLSQAQSLSELEAHPWLMVPGI
                     FIMIAVLSFNFVGDALRDAVDPYRTVEKV"
     gene            complement(12167..13135)
                     /locus_tag="PLU15_07285"
     CDS             complement(12167..13135)
                     /locus_tag="PLU15_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30763.1"
                     /translation="MRAFIARRILIMIPMMLLASVICFWITELQPGDFVSQYLDNPRI
                     SPEQIRLLRERLGLDKPAHVRYFMWLKGVVTKWDFGYSFAYERPVGDLIWERMGWTVT
                     IALLTVVFQWLLAVPMGIYSALHPYSPLDYTLTVIGFIGISVPDFFLALILMYWSLKA
                     GATSIGGLFSPQFIGAPWSLAKFADLLRHLWMPLVVVGLGGLAGLMRVMRGNMLDVLG
                     SPFITSLRAYGLDEQTVKRHAVKNALNPMVSIAGSELPNIFSGTIIAAIVLNLPTMGP
                     FFYNALLNHDQYLVMAFLMFIALITQIGNLLADIALAFLDPRIRMS"
     gene            complement(13203..14972)
                     /locus_tag="PLU15_07290"
     CDS             complement(13203..14972)
                     /locus_tag="PLU15_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30764.1"
                     /translation="MRRRLWLVVVLCLLMTTPALAAPYVEKEDLTPSTKPVRGGTLRL
                     ALPASPQSFLFYGALDNNAYTVIGQTMTGLVELHPVTNAIRPGLAESWETSPDGKEVI
                     FHLRDVKWSDGAPFTADDVIFTFENFIMNPVAKGNSADRFTIVDVTDGKKKPVQWVKI
                     DNRTVKAVLPSPFGPFYMNLSHAYIYPKHKLESKIDKKRPDSVNELWLTNVNPKEIVA
                     NGPYRIASYVMDQKVVLERNPNYWKVDRFGNQLPYFDKLEYLIIKDDEVRLAKFMSGE
                     IDYMAVSAKDFPVLKGKERAGAPFTVYMAQPTQPTPSPVHISFNYDVEDRNLRTLFTN
                     IDFRRAMECLLDRDRIIDEVYNGLAIPGGGLVLPSNKAFYNPKIEEIMRPYDPRKANA
                     ALDKLGLDKRGRDGFRLFPDGKRVEFTLTVASAPQDHQDIALIFKEDLEKAGIKVNLQ
                     ILDATLVGNMFGAGNFEAGIRAFGNQPDLELRKAIWQPGTQLYYWHYSTLDKDNLAAI
                     TKNMTDWEKRVYLLFDRGSVTTDPAKRKEIYDEIQEIYYDVVPVIFVCKGMNLFAANN
                     SLGNIWQDKQGIVYFSSYTTYRK"
     assembly_gap    15698..15797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16007..16855
                     /locus_tag="PLU15_07295"
     CDS             16007..16855
                     /locus_tag="PLU15_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30765.1"
                     /translation="MQIRSEILDTYLRGESTERFSEHWIFQTVDRFLPQGQSAYTPPD
                     ESEEARAAIRRVAQEIREAVDGFVPNNVEVWDALFSGWRLIIDATKINMVVGLPDIYD
                     ALALSDPSGAIQVVYDLPNWLRYEGKHNISDVVGNLLTHELCHVCIGAIYTDIETDSE
                     SADHIMCIDAMAFNEGFAHLVSYNYTPIDKVDWESETLRNVYKKSIARLKLALAETDP
                     PRQQQYLLDAVCGSYYDKYACMAGMLFLAKRWREGGIPALVDEFERGYHGFAQNIVDN
                     TKVSVE"
     gene            16884..17252
                     /locus_tag="PLU15_07300"
     CDS             16884..17252
                     /locus_tag="PLU15_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30766.1"
                     /translation="MLNHFVEVIKAARELSLSPVPVRRLDFDQVIISIFGLRLEADRR
                     TLLFHYFAFLPSSVRSGVVELLVYGLNLGDWDEIQSYKGASKQLPESDDAIFRTSRLF
                     GERYHSPEGWRPPDNLKPLA"
     gene            complement(17385..18506)
                     /locus_tag="PLU15_07305"
     CDS             complement(17385..18506)
                     /locus_tag="PLU15_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOO30767.1"
                     /translation="MKRLASVLFIVSLLVLGSLGALAADDPLLFSGKIVTVDKYGNVL
                     TDISEEAVAASGAEVGDMVIVKIGDKTLVVPFVTIYGDVDRGTPLMRTTNNAIQFAIN
                     YGNFAQTYGASEGTEVSVYLKEKGAYLSEIQIRSLKKLENRDEYDSDRAFANFREVTA
                     GNIKPGILYRVSHPSLGDERSSYAARLVEEHGINTVLNLSDTPEEFEANLAHSEYYRQ
                     LNEKGNIVAVGMGVDFAAKEFTEKFKTVLEFMIDHDGPYAVHCVEGKDRAGLVIAVLQ
                     GLAGATVEEIVEDYMVSYENYYRLEKGTRGYELVSKIMYDQLREMNGGEAVTDANLKA
                     VVVNYLKNMVGLDDDQIETLETILVGEASARAEVVFLAA"
     assembly_gap    19157..19256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19304..20542)
                     /locus_tag="PLU15_07310"
     CDS             complement(19304..20542)
                     /locus_tag="PLU15_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30768.1"
                     /translation="MDLLTNSLFLLSLAILCGHYLGKISLARFNLGASGTLFAGMGLS
                     WLIYDRFVAPYEIAVANGAIDVPSYAVRILQNGFVDKANFDFFLLLFVASVGLLAAKD
                     VARVFRGNGAKFLLLAISITTTGAGVAYSLFRLIGKLDMHAITGLYAGALTSSPGLGA
                     ALESAACMFPDPASAAARAAQASVGMGYAIAYPFAAITVIICMQLLPKLFDVDLQQEV
                     AQLDSDLANGHTWSASKPASFCVPGIAFVSLIGYMLGSISIPLGSVGKVSLGTTGGVL
                     VTGLILGDRGRLRMDERALNVTKQVALYYFLAYVGLNYGYQAATAMTGPNAMLVLVGI
                     LTTATSVLVGFLFGRYVLKMNWIILSGAICGSMTSTPGLGAAIDATGRNEAAGGYGAT
                     YPVGLLCKVVIVQVLSKLVW"
     gene            complement(20590..21513)
                     /gene="sucD"
                     /locus_tag="PLU15_07315"
     CDS             complement(20590..21513)
                     /gene="sucD"
                     /locus_tag="PLU15_07315"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOO30769.1"
                     /translation="MSILIDEQTSVLVQGITGNQGRFHTQQMLAYGTNVVAGVTPGRC
                     GMEVEGVPVFDTVRAATEEYSIDASVLFTPAAAVKDAALEAMEAGIRTLVIITEHVPV
                     HDCLEIMAYADRIGATIIGPNTYGIVSPGRCKIGIAPSSIFLPGSVGVVARSGTLSYE
                     IVGNLTASGVGQSTVVGIGGDRVVGQSFIDILRLFEADSATSVVVLIGEIGGSAEEEA
                     APYITQMTKPVVAYIAGQSAPPGKRMGHAGAIIERGRGTYHHKRRALEEAGVRVAELP
                     SQVTGIVQTELSMLHADPFYGLIWSFSRPQQ"
     gene            complement(21562..22677)
                     /gene="sucC"
                     /locus_tag="PLU15_07320"
     CDS             complement(21562..22677)
                     /gene="sucC"
                     /locus_tag="PLU15_07320"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOO30770.1"
                     /translation="MRLFEYMGKQLFIDFGIRVPRGMVCDTPKTAAQIAQRLGGPVMI
                     KAQVLSGKRGKGGGIRLADGPKESKLAAEQLLQSTVQGFPVELLLIEEKLAIGQELYL
                     SLIVDGVSKKPVLIASAQGGMDIENVPEEQIIRQPIDVELGLPTFLARDIVRRLKIVP
                     SSPCGKALTQVVTALYRLFTAIDAELVEINPLAICGDTVYAADAKITVDDASLYRHAH
                     IPTVSELTLGEQEASKLGLSYVDLDGEIGVMANGAGITMATLDMITYFGSKPANFLDI
                     GGGANQEATRKGLELVLAKQPKAVLVNIFGGITRCDEVAQAIVGIRRQYGKSIPMVVR
                     LAGTNQDAGRAILEKEAGIEVFDLMQTAAQRAVELAG"
     regulatory      complement(22859..23047)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            23237..23953
                     /gene="fabG"
                     /locus_tag="PLU15_07325"
     CDS             23237..23953
                     /gene="fabG"
                     /locus_tag="PLU15_07325"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOO30771.1"
                     /translation="MRETVLITGASRGIGYATAELFAVNGYTVYANYRSSPKSLELLS
                     EKLSERGCTLIPLYADVSNSNDVEHMVSQTNGVDILINNAGIAQFKQFVDMSETDWDN
                     MMSVNLKSVYLCTHCVIPHMIKRKHGAIVNISSVWGVTGGSCEVHYSAAKAGVIGFTK
                     ALAKEMAPSGIRVNCIAPGVIETDMNKELSDYDRQLIKEQTPLGTIGLPDHIAQAALF
                     LAENQFITGEILNVNGGFHI"
BASE COUNT         5529 a         6940 c         6011 g         5461 t
ORIGIN      
        1 ccatatttat tctacgtcgg aatatggttt tcctcctttg gttttgcctt cacaatacca
       61 ccatattaat acaatgcatt gttgagctgc ccatttccat acaagtttcg cattatgtct
      121 cctcttctac atgttggcct cataaactcc attttgcata tataaaccca aaaaaccctt
      181 accaattacc tcttaatggc gtcgccagga ctccctgaat aatctgtttt cgtaaaatct
      241 acacactccc ctgcagaaat gccacaaagc ttatcgataa agatgcccta acagcgatgt
      301 cccgctcagg aacatcttcc gtgaccacgc ggcctctttt tcattcatcc ccatcctgct
      361 ccaaacttgc accaccaatc agattttaat tacactttca catgagacaa tcgaaatcca
      421 aagccgcaaa gatttggaac aggcctaaga ggagcctctc gcaggcgcaa cgaatctatg
      481 cccaaacgga ttatcgctca attgaactgt aggatgatgc ttggcatagt cgcatctggg
      541 aggcgaactt cgagagattc gcacagaagc gacggcttga ctactgacgt atatgaggag
      601 tgtatgcatg acgaatcatg tgttggcagg agtaatggga ctatgcgtcg gcgatgcgct
      661 gggcgttccc gtggaattta ctcacagaaa gactctgaag gaaaacccgg tggtcggtat
      721 gcgcgcctac ggcacatata accagccggc cgggacttgg tctgatgata caagcatgac
      781 gctatgcctt gccgacagcc tcatcagggg attggactat cgggatatca tggagagatt
      841 cctcgcatgg ctcaatgaag gggcgtatac gccatacgga aaggccttcg atgcggggaa
      901 cacaactaaa aaagccctgt acaagttctc ccggggagca acccctttgg agtgcggagg
      961 tacagatgaa cgcgacaacg gaaacggctc gctaatgagg atccttccaa tcctatttca
     1021 cctacgctct gtctatgggc ctggctttta cgacatagat gaggcttttg acatcatcca
     1081 caacatttca gccctcaccc atgcccacga gcgcagccag atcgcatgcg gcatctatgt
     1141 atctatcgct tcagctctca tggaagggaa ggatctcaaa acacacgtcg aaacggggat
     1201 cagaagggcc atgcaatact acatagggca agcccgattt tcccatgagc tccatcacta
     1261 cgaaaggctt gcgcatgaag gcttcaatcg actggatgaa gccgacatca gaagcagcgg
     1321 atacgtcgtc gatacgctcg aagccgccgt ttggtgtctg ctcaacacag gcagtttcga
     1381 ggagtgtgtt ctgaaggcag taaacctagg cgaagatacc gacacagtcg ctgcagttgc
     1441 cggagggctt gcagggcttt attacgggta cgaaagcatt ccgggtcaat ggctttcagt
     1501 gatcgcaagg cgggaatatg ttgagactct ctgcaacgaa ctttacgaat catttcgcaa
     1561 cttcttgtaa acggcattgc ctgtcagacg ttctgacgcg cacgtatacc tcacctttgg
     1621 gatgtggcat aggtgcccga gccggcaaca aagttgccga tagcagcgag ctccgctaaa
     1681 taggggagcg ggaaatatcg ggtgctatct agttccaaac atattccgcg gcactagact
     1741 tggaggggca tttttcatgt atagccatcc tttagagaat cagccgcatg caggcggcaa
     1801 acagcgcgtt taggggctaa agcatgaact tgctctacca ctgctaataa atgagatatg
     1861 gctcccgcgc atccatgaac tcggatgtct cccgttccca cctggccatt atctcatcag
     1921 aagtcgcgcc ctcctcaatc catcttctca actcggcgcc tcctgcaaga agatcgataa
     1981 acagatgccg cccttcgacc gggaacgggc gcgccaggtc agcagagccg gtcggacttc
     2041 ccgcaccata ccatccaaaa gcgtccagat tctgctccct tgcggctatc agcattgaaa
     2101 cccccaagcg aaccggatca agctcccaag catctgtcac ataaacttct accccagcac
     2161 actccacgcc gctgtgcttt gaaaacatgg gcctgaagtg ggccggccta aagcgtacgc
     2221 cgccaagctc catggtgttg agtctatcgg caagatcgat agcatcgagc cagggcgctc
     2281 cgatcagcct gaatggcttc gtcgttcccc ttccttcgga aagcgtggtt ccctcaaaca
     2341 agcaggttcc ggggtacacg agggcggtct caaaaccggg catgtttggc gtgccggtcc
     2401 aaaaatggcc ggtttcgggg aagagcatct cccttcccca gttatccatg ggaatcacga
     2461 gaagatccac gtcaaggcct gccccgccga cgtacatcgc ggccagttcc cccggggtca
     2521 ttccatggcg aataggtatc cgatcgtacc ccacaaaaga gcggtactcg atgtcgagaa
     2581 gattgccttc aacagccatt ccgccgatgg gattgggcct gtccgtcacg attatctgct
     2641 ttcccgcttc ggcagccgcc tcaatacaga gtgccgtggt ggaaaggcaa gtccaatagc
     2701 gcgatccaat atcattgccg tcgcagacca acacatcgat atcttccagc atttgcggag
     2761 tcggcttcac cgtttcgccg tacagactga ccacatctac gccggttcgc aaatcaccga
     2821 catgtcctac cttcaccccg gcctgttcac agccccacaa gccatgctca gccacgtaaa
     2881 tccgggtgaa ctctatcttc ggggactcgg caaagagcct gattatcggg gttgcatact
     2941 catccaccgc gttgtagttg gccacaaggc ccactctcgc cgtgccgaga tacttctccg
     3001 gctcttcgag gagcttcgtc acacccagtt tgaccacggg cccagtcacg ttccacccct
     3061 ccacttcggg ctgctgcagt ccggctcgat tgatcacgcc acagatcctc ctagccccat
     3121 cacttcacac caatccccgg ttctctggca ggcagtgaaa aacgtaccca ggggccaata
     3181 tccccacctt ccgaacacca ctcgaaagcc atctagatct tccttgatgc cagggcatcc
     3241 acggttttca tgaggagctc cattgcctcg tctgattctt tcatggccac tgcggtgaaa
     3301 aggcagtcga tcacggcaag ctgtgtcagc cgagaggtca tcgcccctcc cctgagccca
     3361 gtggcctcgt gtgccgtggt gcagagcacg atatcagagc ccacagccgc tggcgaagtc
     3421 ggaaaattcg tgatgcagac cgtgcacgcc ccgcattccc gcgcaagatc gatgacatcc
     3481 atggcctccc tcgtccggcc tgagtaggtt atcaccaccg ccacatcctc tggattcagc
     3541 agagcagcct gtgtaagctg agcatgggta tcaagatagc tgaaggctgt catccctatg
     3601 cgcatgaact tgagctgggc gtcttgggca accacccctg aggaacctac gccgaagaag
     3661 aacaaccgtc tggcacgatg tatggcctct gctgcgcggg cgacctcgga ggggtcaagc
     3721 accttagctg tatcttcaac cgcctgaagc gttgccgtga atatcttgtc gcagatggcg
     3781 ggtacatcgt ctccaggctc aacttcgccg aacgcaccct ccctgctggt agcgagttct
     3841 ctgaccaacg ccaacttgaa ttgggaaaat cctgtgaatc caaggcgttg gcagcatttc
     3901 accacagtcg actcgctcac tcctgcagct gcagccagct gaaccgaggt catctcggcg
     3961 gcggattcag ggtgctcgag gatgaagaat gcgacttttt gctcggcagg agcaagagaa
     4021 ggatatcgtc cgcggatagt tacaaggcag tagtcggcat tcttagtctg ctgcaccctg
     4081 ttttcatctc tgtcatttgt catctgtctc ctgccccctc aaagtatcct tgtgtagcct
     4141 aaagccgatc ccataccttc tccgaccggc gcccgggcgt tatggctccg agcagaacct
     4201 ggtgatgggc gccggtcgcg ctaggcagat tcgccgggat cccatgtatg ccgagatatc
     4261 cgaggatcgc aaaagcgatg gcctctttgg catcgctcga tatcccatac gtctcgtgag
     4321 ttacaacagg cacaggatcc actcgctccc tgatcatcct catcaaggtg ggattgtagg
     4381 cgccgcctcc cccgatgatc agctcgtcca acccatattc aggcaccacc aagtcgctca
     4441 gatgcttgcc gatgcattca gcggtaaaag ccgtgacaga cgcgacaatg tcctcgtcgc
     4501 cgattcctaa ggcccgcgcc cgagccatga cactctttac atactccgcg ccgaattcct
     4561 ctctccccgt gctcttggga ggctcaatcc gcacaaaagg atgatccatc agctccgcca
     4621 cgaattcctc taccactctg ccccttgccg caatagcccc atccacatcg taatccatgc
     4681 atcccccagt gacttcgctc accaccgcat ctatcaccat gttccccggc ccattgtcga
     4741 aagcgatcac atcctgaggc cgcgcgccgc ggggcaggac cgtcatattg gcaatccccc
     4801 ctatattctg cacaaccctc gtcttgtcct catggcgaaa gaggatgtaa tcggcataag
     4861 gtaccaatgg agccccctgg ccgcctgctg caatatcgcg cgacctgaag ttcgagatga
     4921 cggttatgcc ggtgcgctcg gccactaccg cgggctcgcc tatttggagc gttgaaggga
     4981 cttggaactc cccttctcct gcccagggca gatggcatac ggtctggccg tgcatggcca
     5041 cgagatctac atctcctaat tccaagcccg cctgctctat cacagaaacc gcagcatctg
     5101 caaagagcct gcccagcacg aagttgaacc tgcatattct atccgctgaa gacgttttgg
     5161 gggagaaaag ctcgaatatc ttctctctca ctctctcagg atagtggtaa ttgccaaacg
     5221 ccaacgtctc cgctttcagg cccgaatccc gatcctctat ctccactagg gcagcatcta
     5281 tgccgtcagc ggatgtgcct gacataaggc ccacaacctt catcgttgaa cacaaagcga
     5341 cccgactctc ctccctatag agtcaacccg gccccgcaag cgcatatccc cgggatggaa
     5401 acgggcagcc tccctttggg cgcgatcgcg ccgaagataa gttccgccac ggctcgcatc
     5461 gtgcactccc tgaacccgta ggcggctatg tacgtctctg cagacgggaa ctgttgaatg
     5521 tcgtatggat tcctgagcgc cacaaccacc acggggatgt ttggatcgag cgcctccagc
     5581 aaagccctga ccagtctgcc ttgctctcgg tagaggtggg cgttgtatgt gccgataatc
     5641 accgcatctt tggcgctcgc aaaactgacc gcctccgcta tttcccgttc atcaggggac
     5701 tgggaaaaac cgaaatcccg aaccccggga acaaagcgct tgatcgctgc aacaagcgga
     5761 gattccgcca gttgaccatc ttcgacctgt gtcagccctc ggatgtcggg agacatgact
     5821 acaaggcccg ggtaatcctc cggcgtcatg ggaatgagtg cctgtctatt cgcaacaagg
     5881 gtcaccgaat cccttgcgat ctccagggca acacgacgat tttccgggct tcctaccggc
     5941 ttcaggctgc gtcggacgag gtatctctct ggagcaagca agccgaaacg ttccttgagc
     6001 cccaaaattc gcctgaccga ttcatccacc ctctcctctg agatccttcc actcctcaca
     6061 gcatcccata tgccctcaat cgccacggcc tgctcggcct gattgtgaca gagcagagcg
     6121 atatccgccc cagcctccag cgcccgcact gccgcctcgt cgatggaaaa ccttttcgcg
     6181 atggcgccca tgaagaggtc gtctgtgagc agcactccgc caaaccccag ctcttccctg
     6241 agcaggcccg tgagcacttt tcgggaaaga gtcgccggaa ggtcaggttc cgcctccacg
     6301 gccgggaaaa atacgtgggc ggtcatgacg gccccaaccc ctgctcgtat ggaagccgca
     6361 aacggggcaa actccaccct ctccaacctc tctctgttat ggggaacggt aggcaggtcg
     6421 aggtgggagt ctactgtgac atcgcccttc cccggaaaat gctttgcagt ggctaccacc
     6481 cccgaggcct ggtaagcctc gatagtcgca actccgagcc gggtcaccat atcgacgtcc
     6541 tccccgaatg agcggacccc tatcccaggg ttgttgggat tgctgttaac atcgattacc
     6601 ggcgcgaggt tcatgttaaa tcccaaggca agcatctcgt ccgccgtcac cttggctgcc
     6661 cggcgtgcgt actcctctga ccgggtcgcc ccgagggcca tgttcccagg aaacacagtc
     6721 ccgccccttg tcagccgggc aaccaccccg ccctcctggt cgatggagag gagaaatcct
     6781 attggatggc ggcgcctgga ggcggtctcc tggagcccgt aggtgagaga ctgtgcctgc
     6841 ttcggatcga ttatgttcct tccgaggaag actacgttgc cgatcttgta tttgtcgata
     6901 aattcctgat cagatgacgt aagctcgggg ccatccagac ccaccattac catctgccct
     6961 attttctctt caatgttcat ggaatcaagc agttccattc tacccccgcc tttccatcac
     7021 ccagccaccc aactgcccca tcaactacag acgttatctg gcgcgtggtc ctcgtcgatc
     7081 gagccgcccc caatcgcccg tccggcgaca agcagggcgc caaccgccgg cgaaaatttg
     7141 cctcgctcaa cagaccacgc gggcgccgat accttcaccc tgcctcgcat ggcgtcaagc
     7201 gccatctcgt tggactcgag cacgccgccg acggctgcaa cgttgccagc gccgccgctc
     7261 atcccgagtc gatcgattac cgctaaggcc atcagtccaa gttctcggcc cgcatcgaca
     7321 actatgtcct gtgcgacatg atctcccttt tcggccgcct tggcaactac aggggcaagc
     7381 gccgcgatct ccggtctcgt catccccgaa tacaccactg aaacaacatc ttcaatctct
     7441 tcgatctcga gcgcaacagc gaggtcttcc gtcaaacatg tgggaatctg cctcccgtcc
     7501 gccgcccgga gcgcctcacg gagcccttta actccaatat cgtatgcaga accctcatcc
     7561 ccgaggatgt atccccatcc ccctgctcgc gcctcccggc catcagccga cacgccatac
     7621 gccaaggatc cggtccccgc gatcactact acgccgggct cgcccgcatt cgccccggca
     7681 agcgcaatga tcccatcgtt gaccacggtg agcgcaggaa acggcgcatc cggggaacag
     7741 acttctgcaa gcagctgacg aagcctcatt tcaaccatgt cgcggtcgcg cttacggccg
     7801 gcgcccgcta ggccgaagca tgccgaagtg attctcgccg tcccgccgga acatgccgcc
     7861 tcgcgcatgg ccaatctgac ggccttttct atctcacaaa ccgccgcatc aattccgacc
     7921 gcttgaaggt tggaagggcc agaggatcct cggcctatta caacgccgtc cagggtggcg
     7981 agcagacaat ctgtcctcgt cccgccgccg tctacaccca aaaggagtcc ggcttcgcct
     8041 ccgtgtccaa cgcctagtaa aggaggaaat cgttcagcag gcatttctcc tcacctcgtg
     8101 cgctctatgg ccgcctgtac cgcatttctg gtatgcccac acgccttctc cagagcacgc
     8161 tcggcctcgc gcctattgac tgatgccaac gccataacga tggcggtctt gatgttcccg
     8221 cccgccttat ccagcaaatc ctcgctgacc tcttcatcca gccccgtggc gatggatacg
     8281 attctaacag cacgacgctt cagtttcctg ttggtcgcct gcatgtccac catgaggttg
     8341 tcgtacacct tgcccagctt gatcattgtg gcagtggata tcatgttaag gacgagcttc
     8401 tgggcggttc cggctttgag gcgggtggag cctgtgagca cttcagcgcc gacgggcagg
     8461 gatataacaa catctacatc aaggtccagc ttatcggcgt ggttgcacac gatcgccaca
     8521 gtgcccgcgc cgacctcttt cgccgctttc aatgctccct gaacgtaagg ggtaacgccg
     8581 cttgcggata ttccaaccac aacatccaat tccgtgattg actccgcaac atcgcgaacc
     8641 cctgacgact cgtcatcctc tgcggcttca acagaatgga atacggctcc aggtccgccg
     8701 gcgatcatgg cagaaacctg gtttggatct actccgaacg tggggacgca ttccacggca
     8761 tccaggatcc ccaggcgccc gctggtcccg gcgcccacgt acaggagcct tccgcctgcg
     8821 gccatacgcg ccacgatcat gtcaacagcc tttgcgatct ccggaatagc cctggctacg
     8881 acctcgggca cgcgccgatc ctcctcgttc ataacctcaa ggatctgcac tgtgctcatc
     8941 cggtctagcc cacgggatct ggggttcgtt tcctctgtgg caagatcagc gtagtccata
     9001 ttctccacaa gcaccaccac tttccgcgcc ggtcacgcca gcttgtggcc tggggcgcct
     9061 aactcaacta cctcgcagca acctctgcat ccgcccgagc atgcagatgg caggcgcaca
     9121 ggtgtccatc gttcacctct tgcggctcgg gcttgatctc atgacaaagc tccatcacgt
     9181 gcggacacct cggatggaac gggcaacccg acggcggatt gatggggctg ggcggttccc
     9241 cctcaagctt gactttagcc agcttgcggt ctttcggatt ccagctgggg atggccgaca
     9301 tcaacgcact cgtgtagggg tgagccggtt cgtcgaaaag ctccgaggca ggcgctgatt
     9361 ccacaacatt gccaaggtac atcaccatga tcctgtcgct catgtgatgg accacgtcca
     9421 gattgtggga gatgaagatg agagacatgc ccgtctcccg ctgtatctcc tttagcaagt
     9481 tgatgatctg gctctgaacg gagacgtcca gagccgacgt gggctcatcg catatgagaa
     9541 gcctaggatt gaccgcgatc gagcgcgcca ccgcgatccg ctgtcgctgg cctccggaga
     9601 actcatgggg atatctctgg gcgtattcgg ggtacaagcc cacccgggtc aaaagttctg
     9661 cgacacgctc atccattgcc gccacgtcat cgaagagttt gtgggtctcg atgggttcgc
     9721 ggattatgtc ccgtattgtc atcctcggat tgagtgaatc gaaggggttt tggaatatca
     9781 tctgcacgtc ttttctaaat gtctgcctca catctcggcc aaggcctttt gagatgtcgt
     9841 ggctcgcccc gagaggatca tagtaggtta tcgtccctgc agtgggctcg tagatcttca
     9901 ccaacgtcct cccgagggta ctcttgccgc acccggactc gccgacgatg ccgatggtct
     9961 ctccttcgtt tatctcgaag tcggtcgatt ccagtgccct tacataccct gtaacttgca
    10021 gaaacacccc cgaccttatg ggaaagtact tcttcagccc cgttgtggac agtatcttca
    10081 tcttccatca cccccctcat aaagccagca ggcaacggta tggccaggcg caggctcgag
    10141 atcgggaggc tgaagttccc tgcacttggc cgttgcccgc agacacctcg ggttgaacct
    10201 acacccttcg ggaaactggt cggctctggg cacgtagcct tcgatgtatg gcaggtcttg
    10261 gcccttgtat tctcgtcgga cacgggaggc caaaagcccc gctgtgtatg ggtgcagggg
    10321 gttgtcaaag agctccacaa cggacgcctt ctccactatc ttgcctgcat acatcacatg
    10381 aacccggtcc gcaatctctg ccactgtgcc gaggtcgtga gttatgaata ttatggaacc
    10441 gtcgtactcg ctctggatgt cctgcatgag gtttaaaacc tgagcctgaa tggtaacatc
    10501 cagggccgtc gtgggttcgt ctgcgattat gatcttaggg ctcgtaatga gagccatggc
    10561 tatcatgatc ctctggagca tgcctccgct catctggtgg gggtactcgc tgtagcgctt
    10621 atcgggctcg ggaatgtgga caagcctcaa tgtctcgatg atcctctcct tcgcctcttc
    10681 ctcaccccac ggcatgtgag cttgtgccac ctccatcaat tgttcgccta ctgtgtacag
    10741 ggggtcaaat gacgacatgg gctcctgaaa gaccatgctg atctgcatgc ctcttatctt
    10801 tcgatattcg ctggccgaaa gcccgatcag ctcgcggccg tcaaagacga tgcttccctc
    10861 aagttttgcg ggaggcgctt ttaccagccc cattatggaa tgggcagtca cactctttcc
    10921 acagccggat tcgcccacta aggccaggac ctcgccccgg tttaggtgga aactgacatc
    10981 gtcgactgcg cgcacgatgc catcgggcgt gcggaaatac gttttcaggt tcgacacagt
    11041 gaggatacgt tcgcatgcag ttgtcatacc ttctctaccg tcctatacgg atctaccgcg
    11101 tccctcagcg catcacccac gaaattgaag gaaagcacgg caatcattat gaatattccg
    11161 ggcaccatca gccatggatg ggcttcgagc tcgcttaagg actgggcctg gctgagcagt
    11221 aatccccaac tggtcatggg ctccttgatg cccagcccaa ggaagctgat ggtgctttcg
    11281 cccagtatca tccctgggat cgaaagggtc gaaactacaa tgagatagct caaggtgttc
    11341 ggtatcaggt gcttggtgat gatcttccaa tccgagacgc ccatcacctt cgctgcgagg
    11401 acatattcct tctccctgag gctcaatgtc atcccccgca tcaccctggc cacccccatc
    11461 cagccgatga gggagagcac ggtcacgatt ccaaagtaga cccaggtgct tggccaacta
    11521 ggagggatta tcactgcaag cgccagccac agtgggatcc tcgggaaaga tctgagaagt
    11581 tcggtgaacc gttggatcag cacgtcaacc caacccccgt agtagcctga aagcgcccca
    11641 actatggcgc caatgaacac gctgaggaag gttccgagca gtcccacggt catcgacacc
    11701 ctgccgccta tcactatcct ggaaaacagg tcgcggccaa acctgtcggc gccaaagacc
    11761 agaaacatcg ccttttcagg agatgcctct actccgaaga ggtgcaagtc cgattcccac
    11821 agtccgagga acctgtattt ctcccccctc acgaagaact tcaggggaac tacactgtcg
    11881 ggatcctcgg catataccag ctcatacgtg accggatccc tgctctttac catttcgtga
    11941 atgtggggcc ccacccattt gccagagctg tcaacccact tcaccttgga aggaggcgca
    12001 tagatgaagt ttctgtgcgt ttcggcgaag ttgtaggggg ccacaaaatc ggcaaatatc
    12061 acaacgaggt agaagatgat cagtacaaca aggccaacca tacccagttt gtgtttgaag
    12121 aagctgtcag caattctttt cctggttcct tccatggtgg acacccctag ctcatcctta
    12181 ttctcggatc gaggaaggca agcgcgatgt cggcaagcaa attcccgatc tgcgtaatga
    12241 gtgcgatgaa catcagaaaa gccatgacca ggtattggtc gtgattgagc agcgcattat
    12301 agaaaaacgg gcccatcgtc ggaagattca gaacgatggc cgcaattatt gtcccagaga
    12361 atatgttggg cagctccgac ccggcgatgc tcaccatggg attgagagcg ttctttacag
    12421 catgcctttt gacggtctgc tcatcgaggc cgtaggccct gagcgatgtg atgaatggcg
    12481 atcctaagac atcgagcatg ttgcctcgca tgactctcat cagcccggcc aggcccccca
    12541 agcccacaac gaccaggggc atccaaagat gcctcaggag gtcggcaaat ttcgcaaggc
    12601 tccaaggagc gcctatgaac tggggcgaaa agagcccgcc tatagacgta gcgcccgcct
    12661 tgagcgacca gtacataagt attagggcga ggaagaagtc gggaacggag atccctatga
    12721 acccgatgac ggtcagggtg tagtcaaggg gcgaataggg atgcaaggcc gagtagatgc
    12781 ccatcggcac ggccaaaagc cactggaaga ccactgtgag caaggctatg gtgaccgtcc
    12841 agcccattcg ctcccatatg aggtcgccga ccggccgttc gtaagcgaaa gagtaaccga
    12901 agtcccactt ggtgactacg cctttcagcc acatgaaata cctgacatgg gctggcttgt
    12961 cgaggcctag tctctccctg agcagcctga tctgctccgg tgatatcctg gggttatcca
    13021 gatactgact gacaaagtcg ccaggctgaa gctctgtgat ccaaaaacag atcaccgacg
    13081 ccaggagcat catgggaatc atgatcaata tacgtctcgc tatgaatgcc cgcattcatg
    13141 ccctctcctc taaacgtgca aatagagcag ggggaagcgg ggcgcttccc ccttatcgaa
    13201 cgttactttc tgtacgttgt gtagcttgag aagtagacta tgccctgctt gtcttgccat
    13261 atgttgccga gtgagttgtt ggctgcaaac aagttcatgc ccttgcacac aaagatcacc
    13321 gggacgacgt cgtagtagat ctcctggatc tcgtcgtaga tctctttcct cttggcagga
    13381 tcagttgtaa cagaccctct atcaaagaga agatagactc tcttctccca atcagtcatg
    13441 tttttggtga tcgcggccaa gttatccttg tcaagcgtcg agtagtgcca gtaatagagc
    13501 tgagttcccg gctgccatat cgccttcctc agctcgaggt cgggctggtt tccgaaggcc
    13561 cttattccgg cctcgaagtt gcctgctccg aacatgttcc ctacaagcgt ggcatcgagg
    13621 atctgcagat tgactttgat tcctgccttc tccaggtcct ccttgaagat caaggcgata
    13681 tcctggtgat cctgaggcgc actggccacg gtgagggtga actcgaccct cttgccgtca
    13741 gggaagagcc tgaacccatc gcggcctctc ttgtcaaggc ccagcttgtc gagggctgca
    13801 ttggccttgc gtggatcgta cggcctcata atctcctcta tcttcgggtt gtagaaggcc
    13861 ttgttggagg ggagcaccag gccgccgccg gggattgcga ggccattgta aacttcatcg
    13921 attatcctgt ctctgtcgag gaggcactcc atggctctgc ggaagtcgat gttggtgaag
    13981 agcgtcctga ggttgcggtc ctccacatcg tagttgaagg agatatggac tgggctgggc
    14041 gtcggctgcg tgggctgggc catgtaaacg gtaaagggtg cccctgcccg ctctttcccc
    14101 ttcagtacgg ggaagtcttt ggccgaaacc gccatgtaat cgatctcacc cgacatgaac
    14161 ttggcgagcc tcacttcgtc atccttgatg atcagatact ccagcttatc gaagtatggg
    14221 agctggtttc cgaacctgtc taccttccag tagtttgggt tcctctctag gacgactttc
    14281 tgatccatta cgtagcttgc gattctgtat gggccgttgg ccactatctc cttgggattg
    14341 acgtttgtaa gccacagctc attgactgag tcgggcctct tcttgtcgat cttgctctca
    14401 agtttgtgct tggggtagat gtaagcatgg gaaagattca tgtaaaacgg cccgaatggc
    14461 gacggaagaa ccgccttgac tgtcctgttg tcgatcttga cccactggac aggtttcttc
    14521 ttcccgtcag ttacatccac gatggtgaac ctgtcggcag aattaccctt ggccaccggg
    14581 ttcatgatga agttttcaaa ggtaaagatg acatcatcgg cggtaaaggg agctccgtca
    14641 gaccatttca catctctcag gtggaagatg acttcttttc cgtcgggaga agtttcccac
    14701 gattcggcca gacccggcct gatggcgttt gttacaggat gcaattcgac aagtccggtc
    14761 atggtctgac cgatgacggt gtaggcgttg ttgtcgagcg ccccgtagaa caggaacgat
    14821 tgcggggaag ccgggagcgc gagacggagt gtgcctccgc ggactggctt tgttgaaggt
    14881 gtgagatctt ctttctcgac ataaggtgca gccagcgccg gggtggtcat cagcaagcag
    14941 agaacgacaa ccaaccaaag acgacgtctc attgaaagac cctccttatg ttaaggtatt
    15001 agctaagaac ctgggttatc catgacatct cagacacagc atcccttcgc atcgcatcag
    15061 tcccttccga gcaagtttcc gcttgataac gtctcggtac atcccccgcc cgcgcggggg
    15121 taactgacag catttgctac gcctaatcgg gtcaatccag taataccaat tcggcctcca
    15181 tgcgggaaat ccttccgatt aggaaaattt atttgcattg tatgtcttta tcggaataac
    15241 ttattctcga gctttggcga aggcttggaa ttgatatttg ctgcatgctc cgtgctcgcc
    15301 gtggactagg acagattcga tcagcacgcg agacaagaga atgttgcagc cccaaacacc
    15361 ttcgccctgt caagctttgc gccagaaggg actgtaggtt ccatgaatcc cgacgccctc
    15421 ctcttcacat gatcgatagc agatcagagt cgctagagac cacgcgcgac gatgttctcc
    15481 aaagcctggt tgaagcctgt taggtctcaa gccattcgca gcgaccggag acaaaatcct
    15541 catttgagta tacttgcgcg aactaaagcc tacagaattg aggatgaagg cagttgaaat
    15601 cccagttatt gagcacttga cagggatata acctacgtgc gtattcagcg cccaaggcgt
    15661 ctggcattag cacgacagcg gtcatcggcc aaccgcgnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnagt attgagcagc atcaatttga aggcgaaatg tgagccaaag
    15841 gatagctgcg ataggcggac taggcaagcc tccccatagc ttaccttgca cacgatctga
    15901 ggaccgatcg aacacggtag tgggcaagga attgtgtggc aaatggcgaa tgcgataaat
    15961 taaatactat ccgacgaaga accgatgcgg ataggaggtc ctctatttgc agattaggtc
    16021 ggaaatactc gacacatacc ttcgcggaga atccactgag aggttcagcg aacactggat
    16081 attccaaacg gtcgacagat ttctccccca gggccagtcc gcatataccc cacctgatga
    16141 gagtgaggag gcccgtgcgg cgattcgccg cgttgcgcag gagatcagag aggctgttga
    16201 cgggtttgtt cccaataacg tggaagtatg ggatgctttg ttttccggct ggaggctcat
    16261 cattgacgca accaagatca acatggttgt aggcctcccc gacatatatg atgcattagc
    16321 tctttcggat ccttccggcg ccattcaagt ggtctatgat cttcccaact ggctgcgata
    16381 cgagggaaag cacaacatat ctgatgtagt cggcaacctg ctcacacatg aactgtgcca
    16441 tgtatgtatc ggtgccatct acacagatat tgaaaccgat tcggaatctg ctgatcatat
    16501 catgtgcata gacgcaatgg cattcaatga ggggttcgcc catttggttt cctataatta
    16561 cacacccatc gataaagtgg attgggagtc tgaaaccctc aggaatgtat acaaaaaaag
    16621 cattgcgcgg ctgaaactag ctctggcaga aaccgatccg ccaaggcagc agcaatatct
    16681 gcttgatgcc gtgtgcggca gctattatga caaatacgcg tgcatggcag gcatgttgtt
    16741 cctcgctaaa agatggagag agggcggcat tcctgcgctt gtcgatgaat ttgaacgcgg
    16801 ctaccacggg ttcgcacaaa acatcgtgga caatacaaag gtgagcgtcg aataatcacg
    16861 ctctctggct gcttgggtgt tggttgctca accatttcgt cgaagtcata aaagcggcaa
    16921 gagaactatc attatctccc gttcctgtac gcaggctcga cttcgatcag gttatcatca
    16981 gcatcttcgg gcttcggctg gaagctgaca ggagaacgct gcttttccac tattttgctt
    17041 ttctcccctc tagcgttcgt tcaggcgtcg tggagttgct ggtatacggt ttgaacttgg
    17101 gggattggga tgagatacaa agttacaaag gggcatcgaa gcaactaccc gaatccgatg
    17161 acgccatctt caggacaagc cgcctttttg gcgaaaggta ccatagcccc gagggctggc
    17221 ggccgccgga caacctgaag ccattggcat aacagcggga gctgccgggc tccatgggct
    17281 tgccatagat cagacagctc ccgcattgac tattctggaa tctactcaat cgcgagtatg
    17341 tacagagaac tccacttctt agagttcggg attcatgaag tgctttaagc agccaagaag
    17401 acgacttctg cccgggccga tgcctcgccg accagtatcg tttcgagtgt ctctatctga
    17461 tcgtcatcca gccccaccat gttcttgagg tagttcacta caacggcctt gaggttggca
    17521 tcggtgaccg cttcgccgcc attcatctca cggagctggt cgtacattat cttggagact
    17581 agctcatacc ctctggtgcc cttctccagt ctgtagtagt tctcatagga gaccatgtaa
    17641 tcttcgacga tctcttcgac cgtcgctccg gcaaggccct ggagaaccgc tatgaccaat
    17701 ccagccctgt ccttcccttc cacgcagtgg acggcgtacg ggccatcgtg gtcgatcatg
    17761 aactcaagga ccgtcttgaa cttctcggtg aactctttcg ccgcgaagtc cactcccata
    17821 ccgactgcaa cgatgtttcc tttctcgttg agctggcgat agtactcgga gtgcgcgagg
    17881 ttcgcttcga attcctctgg ggtgtctgac aggttaagca ccgtgttgat gccgtgctct
    17941 tccaccagcc tggctgcgta tgaggaacgc tcgtcgccga gtgacgggtg ggagactctg
    18001 tacaagatgc ctggcttgat attccctgcc gtgacttctc tgaagttggc aaaggccctg
    18061 tcactgtcgt actcgtcgcg gttctcaagt ttcttgagac tgcggatctg tatttcagac
    18121 aggtaagcac ctttctcctt cagatagacc gatacttcag tcccctcgga agctccgtat
    18181 gtctgtgcaa agttgccgta gtttatggcg aactggatag cgttatttgt cgttctcatc
    18241 aagggggttc cccggtcgac atccccatag atagtgacaa agggaactac aagtgttttg
    18301 tcccctatct tcacgatgac catgtcccct acttcagccc ccgaagctgc aacagcctct
    18361 tcgcttatgt cggttagcac attgccatac ttgtcaactg tgacaatctt gccgctgaaa
    18421 agaagaggat catctgccgc gagtgcgcct agcgaaccca agaccaaaag ggaaacgatg
    18481 aacagaacag acgccagtcg cttcaatctt aatacctccg cttcgcctga tttttgggag
    18541 cttatgcctg gaccgggact tcctttttcg aattgagata gatccaagcc gccccaggaa
    18601 ttgactctag caagtcctat tatagcatga taggcggcga catatcgggt tttggtcggc
    18661 ggcacccggt agatgttgcc caattatctg ccagatgtag gaccggggag aagcaagcga
    18721 gcccgtttgt gtgttaacca tactagtgct cgcgcttaga cgcgaaaggg ctgagtttcc
    18781 tttccaatgc tctgtgcctc gatacaccag catcaatgtt gcgctgctca gcatgcgtca
    18841 ggtaaaatca tggaacaacc cagcagataa ccgttggcat ttggggattt gccatctata
    18901 tcgggcatca cagtgaaatg cctaaacttc ccgatgtatc acatgatggg tcgaaaggtc
    18961 gatctgtata cctggaactt cgatcgcatc taagatcaag caggttctcg ggcttcacta
    19021 cgagtcagat tgcgccaaat acgagtttgg ccgggactcc ccttgcattc tccaaagggt
    19081 ttggctctag caaaatcaac agcgcaggtg aatgatgcga aagtagaaat taactccaaa
    19141 ttctcgcaag gtctccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaacc
    19261 gatggctgat tatgattact gcttattcgc ttttccaccg gccttaccag actaacttgc
    19321 tcaatacctg cactatcacc accttgcaga gcaaccccac aggataggtg gcgccataac
    19381 caccagcagc ttcgttacgc ccagtagcat caatagctgc gcccaggcca ggagtggaag
    19441 tcatgctgcc gcatatagcg ccggacagaa tgatccagtt catcttcaac acataacgtc
    19501 cgaacaagaa gccgacaaga acggaagtcg ctgtagtcag gataccgacc agaaccaaca
    19561 tggcattcgg tccagtcatg gcggttgcag cctgatatcc gtagttaagg ccgacataag
    19621 ccaagaagta atacagagct acttgtttgg tcacgttgag ggcccgctca tccatacgca
    19681 gacggccacg gtcgcctaga atcaggcccg taaccagcac tcctcccgtg gtgcctaaac
    19741 tgaccttgcc cacggaaccc aagggtatac tgatgctgcc cagcatatat ccaattaggg
    19801 aaacgaatgc tatgccaggt acgcaaaacg aggcaggctt ggatgcagac caggtgtgac
    19861 cattggccag gtcgctatct agttgggcaa cttcttgctg caaatccaca tcgaaaagct
    19921 tcggaagcag ctgcatgcat atgatgactg taatcgcggc aaacggatag gcgatggcat
    19981 aacccatgcc tacgctggct tgcgcagcac gagctgccgc tgatgcggga tccgggaaca
    20041 tgcaggcggc agattccaga gccgcaccaa gcccaggcga gctggtcagc gcaccagcat
    20101 acagtccggt tatagcgtgc atatccagct ttccgatcag gcgaaaaagc gagtaggcca
    20161 cgccagcacc agtagtggtt atggaaatgg ctagtagcag aaacttggct ccattacccc
    20221 taaaaactct tgctacatct ttggcggcga gtaatccaac tgaagccaca aatagaagca
    20281 ggaagaaatc gaagttggcc ttgtccacaa atccattttg cagtatgcgc actgcatagg
    20341 aaggaacatc aatagccccg ttcgccaccg cgatctcata gggagccacg aaacggtcgt
    20401 atatcaacca gctcaaaccc atacccgcaa agagtgtacc ggatgcgccc aaattgaaac
    20461 gtgctaggct gatctttccc aagtaatgac cgcaaaggat ggccaagctc aatagaaaaa
    20521 gtgaattcgt cagcaagtcc acgtgtcatt catctcctgc cttggtcacg ccgtgcaaag
    20581 tgtttcactt tactgctgcg gtcggctgaa agaccagatc agcccataaa aaggatctgc
    20641 atgcaacata gacaactctg tctgcacaat gccggtcact tgcgagggca attcggctac
    20701 ccgtacaccg gcttcttcaa gcgccctgcg tttatggtga taagtgcccc tacctcgctc
    20761 gataatagct ccagcgtgtc ccatacgttt accaggggga gcactttggc cggcgatgta
    20821 ggcaactaca ggcttggtca tctgagtgat atagggtgcc gcctcctcct cggcactgcc
    20881 tccgatctct ccaattaaga caaccactga ggtagcagag tcggcttcaa aaagccgcag
    20941 aatatcgata aatgattgcc ccacgacacg atcgccacca atacccacta ccgttgattg
    21001 accaacgcca gaagccgtga gattgccaac tatctcatag gatagcgtgc cagagcgagc
    21061 gaccacacct acactccccg gcaaaaagat agagctaggt gcgatgccta tcttgcatcg
    21121 gccgggagag acaatcccgt acgtgtttgg gcctatgatc gtggccccaa tacgatctgc
    21181 ataagccata atctccaggc agtcatgtac cggtacatgc tcggttatta tcaccaatgt
    21241 tctgatgccg gcctccatag cctccagtgc tgcatcttta actgcagctg ccggtgtaaa
    21301 gagcaccgaa gcgtcgatag aatactcttc tgtcgctgca cgtacagtat caaaaacagg
    21361 aacgccttct acttccatgc cacaacggcc aggagtgact cccgccacta cattagtgcc
    21421 gtaagcaagc atctgctggg tgtgaaaacg gccctgatta cccgtgattc cctgtaccaa
    21481 caccgaggtc tgctcatcaa tcaggataga catgctaaac ctcctcaata tagtagaagc
    21541 aatgctctgt aactggttga cctaacctgc taattccact gctcgctgag cagcagtttg
    21601 catgagatca aaaacctcga ttccggcttc cttctcaaga atggcacgcc cagcatcctg
    21661 atttgtgcct gctaaacgaa ccaccatggg aattgatttg ccgtattgtc tcctgatgcc
    21721 gacaatggcc tgcgccactt cgtcgcagcg cgttatgcct ccaaagatat tgacgagtac
    21781 ggccttgggc tgcttagcca agacaagttc cagtcctttt cgggtagctt cttggttagc
    21841 tccgccgccg atgtccagaa aattagctgg tttgctgccg aaataggtta tcatatcgag
    21901 cgtagccatg gtaattccag ctccattggc catcacacct atttcaccgt caagatccac
    21961 atatgatagt cccaacttgc tagcttcttg ctcacctaaa gtgagctcac ttaccgtcgg
    22021 aatatgggca tggcgataca gcgaggcatc atcgacagtg attttggcat cggctgcata
    22081 gacagtatca ccgcagatcg ccagcggatt gatctccacc agttcagcat ctatggcggt
    22141 aaacagtcgg taaagcgccg tgactacttg cgttagtgcc tttccgcagg ggctgctggg
    22201 cacaatttta agcctacgga caatatctcg cgccaagaaa gtaggcaatc caagctctac
    22261 atctatcggt tgtctgataa tctgctcttc tggcacattc tcaatgtcca tgccgccttg
    22321 ggcagaggca atgagcacgg gtttcttact tacaccatca actatcaagg acaaatacag
    22381 ttcctgcccg atggccagtt tttcctctat caaaagcagc tccacaggga agccttgcac
    22441 tgtagattga agtaactgtt cagccgcaag ttttgattct ttggggccat cagccagccg
    22501 aatacccccg cctttgcctc ttttacccga aagaacctga gcttttatca ttaccgggcc
    22561 acccaacctc tgtgctatct gcgcagcggt cttgggagtg tcgcacacca tacctcgggg
    22621 cacacgtatg ccaaaatcga tgaataactg ttttcccatg tattcgaata atctcatctg
    22681 atcatccccc tcgtgtctga ataactccta ctctcgttac aagaacacta catgagctga
    22741 ttgagctagc taaataagaa agtccctcac gccgtgaagg cataagggac cgactaacct
    22801 aggccatcta tcttgcatcc gatgcctggt atatcgcact cctcctttcc tccgaaggcg
    22861 gcgtactttc tggaagggca ggtctcctgg cttacgctca tcgttctgct gacccttccc
    22921 gtctatcatg cattagacag tggcatgcca gcgaactcac gcttacagtg gcgggaccgc
    22981 tcaggacttc cacctgattc cctattctcc ccgggaactt cggggcacca cttccattag
    23041 atgtttagtt ttcatgttta attatatcat taccatttga aactaacaat gagttggaaa
    23101 ctgtatattg ggcatctgtg gtattgccgc ccatgaacaa ggaggacatt atattggagg
    23161 agaaaaagct acaatactga atagagatat ttctgaggaa acacagccat aggcggcctg
    23221 gaggttacat agagccatga gagaaactgt tctgattacc ggagcgtcac ggggaattgg
    23281 gtatgccact gcggagttgt tcgcggtcaa tggatacacg gtctatgcca actatcgcag
    23341 cagccccaaa agcctggagc ttctttccga gaagctctcc gagcgcggat gtaccttgat
    23401 ccctctttat gcagatgttt cgaatagcaa cgatgtggag catatggtct cgcagacgaa
    23461 tggagttgat attctcatca ataatgcagg gatagcgcag ttcaagcaat tcgtcgacat
    23521 gtccgagact gattgggaca acatgatgtc tgtgaacctg aaatccgtct atctgtgcac
    23581 tcactgcgtc attccacaca tgataaagcg caaacatgga gctattgtaa acatctcttc
    23641 agtgtggggt gttacgggcg gttcttgcga ggtacactat tccgcagcaa aagccggagt
    23701 gatcggcttc actaaggccc tggccaagga gatggcccca tccggaatac gcgtgaattg
    23761 catcgctcca ggagtaatag aaacagacat gaacaaagag ctatccgact atgacagaca
    23821 gttgataaaa gagcaaacgc cattaggaac gatcggcctt cccgatcata ttgcacaggc
    23881 agccctcttc cttgcagaaa atcagttcat taccggagaa atactcaatg tcaacggagg
    23941 gtttcatatc tgacatgtcg catacgcctg aaatcgtgtt ccctctgcaa actgttctgc
    24001 tgggcgaaag gtacgatggc ccttaaagct gggttccgga acagatcatc caggcccatg
    24061 gaattgcggg gttggcactc gatggctgcc gctcggcaat ccagagccat tgacatgaca
    24121 tagaacctac cgggctccgt a
//
LOCUS       DAOSYL010000039        23833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1754_length_23833_cov_8.706283, whole genome shotgun sequence.
ACCESSION   DAOSYL010000039 DAOSYL010000000
VERSION     DAOSYL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23833
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_23833_cov_8.706283"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..586
                     /locus_tag="PLU15_07330"
     CDS             35..586
                     /locus_tag="PLU15_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HOO30772.1"
                     /translation="MNMAELTAESGKALKGLLEAARLKPHDIVVVGCSTSEVMGRKIG
                     SASSVDVAEAIMAGLLPVISENQLYLAIQCCEHLNRALVVEQECAERYRLERVTVIPH
                     VAAGGALAAEAMKRFADPVVVESICAHAGLDIGDTFIGMHLKRVAVPVRLEVTKIGAG
                     HLTAARTRPKLIGGERAHYPTRS"
     gene            2407..11256
                     /locus_tag="PLU15_07335"
     CDS             2407..11256
                     /locus_tag="PLU15_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO30773.1"
                     /translation="MACGYRKSPRLRATLLVVFLTLLTVGLGSQGALGFEAKMGGGWQ
                     PGPDEYHPSRLIVEFDTGIRIQAAADSVHKLGYSLESVQSFVPTEKFPGGLSVGIVDI
                     PESETVDRAIARLSGAPGIIRAERDYKKYKCSEPVFPNDIYFPQMWPLHNHDLPENYR
                     DPEMPWYYDPVNDADIDAPEAWAMFKGSSEVIVAVIDTGTYIDHPDLADHIWVNEAEL
                     YGEPGVDDDGNGYIDDIHGWDFYNNDNSVFDRDERDKWGDLNDEHGTHCSGTIGAMTN
                     NGIGVSGINWNVTIMPIKFLGPDGGYTSDSIKAIRYAAANGATIASCSWGGGGYEQIV
                     KDVIEASGMLFLCAAGNEGVNTDLEPHYPSGYDLPNIISVGASMQNDEPCYYSGWWGS
                     NYGAKTVDLFAPGGFILSTLPPDPVPDEPAEKYAFFYGTSMATPHVSGVAALVAGQNP
                     DMPLYPGAPGWKPGDRTVRDIILGSVDVKSAFKGKCVTGGRLNAANALRAASGPVIVS
                     ATAQPAYGQPPLDVVFSASATTPVGVIVDQWWDFGDGSAVVHEFDAQHRYSTVGDYNA
                     SFHVVNDLGIESVATVEINVCIPPVIEINPTSVESNLAWGETQTRQVTITNTGEGELN
                     YSVGVELFGSSQEGSATEPRGWGGPDDFGYIWIDSDHPAGPEFDWIDISRIGTALPLQ
                     DDGGQTVPLPFEFPFYGKAKTKIGIASNGYLSFGTWLSTWRTTPIPDSTQPNDLLAVC
                     WIDLDPQRSGMVYYWGDDSKFIVEWQDVPGTLAGGPYTFQAILTPDGAIKYQYKSMLG
                     THTNECTIGIENSSGTVGLQVARDELYVHDELAILFSPTWINVSPVEGTVAPGSSAML
                     DVRFDAGQLPRGSFPAAIRVKSNDPIAPEIVVDTCLNVNPIMPPVITSIEADPRGGAA
                     PLEVNFTASAYDLYGEVVRIVWDFGDGSDPVEGTLTATHTYTAEGEYDAVLTVVDDDG
                     LTATATLRIAVRALPKAEVNPPEIRQAIRAHRTRTERITVANTGDADLVIEACAAACG
                     SPGEEPEQFIKEGSSGQDKFGYIWRDSNMPGGPKFEWAEISEIGTKLTTLKSNQYEEI
                     ELPWEFSFYGQPKTSVKVNAAGFLTFGPTLGSPTSNTPLPDIGFPNELLAVWWDTLRP
                     ASAPQDGGVFHYYDAENDRVIVEYKDVPRSWNSGQLTFEVMLYPDGRIVYQYLDMSFG
                     NDADEASATIGIENADGTDGLEVLCDAYGYIENNLAIEFRTYQWLSVSPGSATIEPGQ
                     SQVFDVVMDLASVPPVDLDGYVVFDTNDVRAPRTIVPVHVSVIENIPPVISACAVNPS
                     QAPAGTVFQFVAAADDLDGTIVDKYWTFGDGAPVVHEFTAEHEYVEEGTYVATFTAVD
                     NDGYKTSVSIRVRVQDAPCASWTPGQFRFTAAQRQTVSDILILHNTGEGELVFGSAPA
                     SILPQAEDVMLPESTEDEYAMDTRGSIEPERDNARSPWLPENIGRVIKSWTCPWPMTA
                     TYGVAVNYNTGELFLSDSLLKNDLIVTPDGKWTERGWAVKFPTTFPADMAFDGQWLWQ
                     VGVGSGNPIYKMDPATGEVQGSISNRVWSAKAQRSLAYNPNDDTFYIGGWNDRIIYHV
                     KGETWDVPGEVLDKWPMPVPISGLAYHPVANILAVTTNANPDMVYFVDCESHATVAQF
                     PHPYAYYADYTGAGCSFDADGNLWLVSQANNSMYLVETGLGPIAADWLSWAPENGRVE
                     PAGSAAVTVTVDTTELVPGTVKGSVILYTNDAEYPMIMVPVVATVKAAPRITECTVTP
                     VIGEPPLEVTFHAKYEVPETPVASYGWDFGDGTTYEGLDAVHTYAAEGTYEAVFRVVD
                     AIGGVDEARTTIEVKWLPCATVSPQEINITLAPNEMASQTVTLGNVEGNAPLNYSIKV
                     QRKSAPFVAMPEVLQSVADPYARTAADLSVPFDQSIVAQIAENVKPQGAGNLVTSWTI
                     PSVAEGPWGIGVEDHHVWISDTRLIADHKMTKDGEYTGVSFETPWIGFGGWPADMAFD
                     TVRNIMWQVNTAGDLGIYGIDPATGEVVDRIISNELWAKMSQRGLAYNPDDDTFYIGG
                     WNQKIIFHIKGPSWDQPGAILEQWSFPVNIAGLAYHPAGVLWVTDNGQPDMIYGIDVE
                     THKVIEQFLHPAYLYNSGAGAEVDSEGNLWVISMDEKMVYVVSTGLPPMPPIGVDPGS
                     GSIPAGETCEITVEFDALKLGRPGDDVERYLEIVTDDPMVQSLNVKLNIHIEGGPVIS
                     KVTATPAIGQPPLAVVFDGLVEPGDVPITDVWWDFGDGSESVHDAHAEHTYTSVGQYE
                     AELHAVDENGVSASEKVTVTVKWLPVFNVEPMSFDEVVLAGTEAQSLLTISNTGVSAM
                     DFAVTTMPSFAQSPEWKRYAEMEPVKGESASRPIGFAGAGAGGPDQFGYVWMDSNQEN
                     GPVFDWFEIREVGRRIELFDEDGVTVPLPFKFPFYGSLKTQVGIASNGYLSFDQSQLK
                     GHFRNSPIPNSADPNDLMAVYWTDLDPEAFGDVYYYHDELNGRFIVEYDNVPEWGDEI
                     GSTYQVILYPNGTIVYQYLSINGDPEAVTVGIEDAIGADGLQVVYNAAYLEDGLAVAF
                     SPAGSVVRVNPDSGHLVPGGKQDVVVTLGSPDAAPGTYSLYLYVTSDDPFRPCATIPV
                     TVKLNQAPTVTIVSPAASDKWEGTKEIVWKAADPDDPADSLSVDIAWTRDGETWNEIA
                     SALPNSGSYEWDTKEVGKSGDSFRVRIVVSDPAGESAEAVSGEFAIEVLNVAPEVKIL
                     KPEAGAVWTGTRAIEYEAVDADGDTLAILLEYDCLDDEAGWQLIADGEENTGKYLWDI
                     SKLAKGGLYKVRVTATDPDGAEGHDVSDEFTVIVLAHAVVAAPNPARQSVTFYYDAET
                     DGKLYVYDVAGRLVYSAELTAGSTFHMWDLTLGGRPVASGLYLYVVVTDGGETSEVGR
                     LVIQR"
     gene            11374..12258
                     /locus_tag="PLU15_07340"
     CDS             11374..12258
                     /locus_tag="PLU15_07340"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOO30774.1"
                     /translation="MQRKISAFLLAVTLVLAMSAAAFAAEDADPIGTAAMLDIGMGAR
                     AMGMGGAHIAVADDAAVIYYNPAGLARIEGRSVTSLYTSLHGAAGYMALGYAQKNVGG
                     GFLRLNASGIEETDEFANVIGTFGMSDFTAMAGYGREVVPGLSLGGTVKLYSQSLPDV
                     SGKGVTGDVGVLYVMDDAKLSFGAVARNLMGKVRYDNIAEDAFERAFGVGAAYRPSDS
                     LLFAADAVFEDGLAARVGAEYRVKQFALRAGGVLDGDETSLTAGAGFAMEGFSVDYAY
                     QTHSVLPDSHRVSLNVRF"
     gene            12696..14243
                     /locus_tag="PLU15_07345"
     CDS             12696..14243
                     /locus_tag="PLU15_07345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO30775.1"
                     /translation="MGYAIVGVLIVSGIILLGISIYVAIEGRRRRNLWFAGFALAAAL
                     HSFGYAFKVTANSVEEALVYIRIAYLGISFLPTFGFLAALELAGKDRHLKKGTILSMI
                     AFSAVTLAIVCTNSLHHLYYIDIVLTKANVLTIVHVVPGIWYKVFMTYVNLAFAAGIV
                     IMARALAEAPVDLKPQYWVYFVSFLVPGVGNLVYLLGLSPYDAYLAPFYLMFMSVLLL
                     FGLNENQLFQVVSIAKSRVLDSMDDAVLIINRNNRIIDHNHSLTHIVAGNSDDYVGER
                     LSDVFSSHPQVVDFVTNHANGQTNVALQDGSGRVYRVRVRTVQGRRKGAFVKCLTMSD
                     MTREMNVMRDLKDLAHRDALTNIANRRALYCQAEDVLRRCGTGMAVSSIMLDIDDFKQ
                     VNDSYGHEAGDMVLKEVALLLKQSIRAQDILARYGGEEFVLIMPDTEVDAAFALAERL
                     RKKIAGAEIFYNDQRIELTVSMGVAGEICSPDLQVDQLIKNADAALYEAKRSGRNKVA
                     LYRDCDG"
     gene            14929..16743
                     /locus_tag="PLU15_07350"
     CDS             14929..16743
                     /locus_tag="PLU15_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOO30776.1"
                     /translation="MSLWDRSMCEFMQQVAEAVSTVLQLDVTIVERGLTRVAGTGRYS
                     RLVGQKVASSSVFAESMRTGKPRLITDPRRNVACGKCEVAEKCIETCHMVYPLMMDGE
                     PLGVVGLICFTEDQRRRVVNCSDEYVAFMEQMARLVESGAKNAYVTQEVKKSRDQLTG
                     IVNAVDEGIIAVDDDGIVLCCNRAASKVLGLDGVELVGKKLGDVVADAPILEVVQTRT
                     PYKGKEVIVSVPAGRIQYVSSGSVLRNGGCVVGAVELLTSSKNARRFAYQVSNSQPDQ
                     PIDNILGTAPALMEAKKMALSTAASFSTVLLIGESGTGKELFARAIHYHSARRYGPFV
                     TVNCAAMPEELLDSELFGYEEGAFTGARKGGKPGKFELADEGTLFLDEVADCSLRLQA
                     KLLRALDDGEICRIGGTQTLTSNARIIAATNRNLEQMVRQREFREDLYFRLNVIPIRI
                     PPLRERKTDIIPLFSYFLNKHAILSQRDIPVLCEEVEQILLVYDWPGNIRQLENATQY
                     VLHTCLGHTVEPHHLPAQVTKGTMEHGGSSTGDIANETGSQAFNWFVSKSEWERMAIA
                     EGLRQLGHSGKAKETIAERLGISRATVYRRIREYDLGG"
     gene            16970..22702
                     /locus_tag="PLU15_07355"
     CDS             16970..22702
                     /locus_tag="PLU15_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO30777.1"
                     /translation="MAKARRPNWASMAIIMVIVVFFAASVESASTASPVDAGNAWAQQ
                     ELAGEAFEDGEYLVRFIDGVGSSEVTGSLDRIGMKIVKPIWFKPSDAFPQGLTIYRVK
                     VCDGQRINAESVVEKLGANPQVMYVSPQRKVYAYDSPSAVIPDDPLFPRMWGLNNTGQ
                     ESYPGLVGTPDADIDAPEAWAIRHDVPEIVVAVLDTGIEWDHPDLMDNIWINSGEIPG
                     NGADDDGNGYIDDIYGWDFANDDNSVYDGLDSHGTHTAGTIGAVGNNGLGVTGIAWNT
                     KIMCLKFLEDGVGDTGNAIEALAYAWNNGAHITSNSWGYSGPLDPALEDAFRACDLLH
                     ICAAGNTGSDNDSKYPKQSHYPSSFPLDNIIAVAATDCNDRLAAFSCWGKTTVDIAAP
                     GHRILSTLPTDQTPEGYLPYAWMNGTSMATPHVAGACAVLMAEFPDMPLYPWGSDWQD
                     DQVTIKELILSTADKKPDLEGVVLSGGRLNICNALRQQVPPKIANVSGFPPYGSPPLA
                     VTFAATVEDEANILDAWWDFGDGSEAVHAYTASHVYGQTGMYTSTFYAIGRNGIGANA
                     SVRVTVYDEGSVIFVDDDGGASDSEVFLNALNGIGVPWVLVEPPVPFSPDISNPVIWT
                     TGRTMQRTLTSEDQIWLSQYLDNGGRLFLCGQDIIWDLGRDNAFVQQYLHVAEYVKDV
                     RTDNVYGIPDDPLTDGMVIKLDYPFKDYSDTVLPDKYATPIFSNDSGRPCALKYYGPD
                     TYRLVFFVFPFEAIPDIKWTDGENADTGIEATATDVMAAIYEFISEEGDPKASVCPES
                     FDITLARGSSASEVLAISNTGESRFFFELGIDRSDAGNPGGDGPFGRRDTYGYIWRDS
                     DSPEGPEFKWVDITDRGTLGVLGDDYSIDIELPFEFMFYGEAKNRVTISADGFLTFGV
                     TGSAAVNTALPDRALPNDLIAVFWDDLQVTDGGVYYYYDAANERFIVSWVGAHRIENK
                     VPEGCFNFQAILYRNGKIVYQYASMDGTMTNATVGIEDRSGEVGLQVICNAEYLHSNM
                     AVELIHEADGISFGRRIGAVRPGEMIDIEVVIDAQMLDEGKYARQILVNIDDPVNPVI
                     AVPVNIAVTGNELPVVTKATVTPDHGEPPLTVMLEAGAYDSDGELADLWWDFGDGTPP
                     EHDLQTTHVYENPGFYEASFHAVDNLGASTSVTVPVRVEGAAYASVKPDALVVSVIPH
                     STATRRLEIQNSGTSPLRFMVTGFKTNEANFTGYERKAVLDGWDPNAYDSGDQFVIED
                     RTITKLDIGDKVQSWPINGVFRAWGCGYDGENVWFTDLRKRDWQFTTEGEKTGVTFEL
                     PWVGQWAADLAWDGEYLWHVNVGGDNRIYCIDPADGEVVTSIRDPYGIWDATSQRGIA
                     YDPVEDVFYIGGWNQLVIYKIAGLSWPRPGEILRTYDDSTLPVAGIARVGRYLVLTLN
                     GRPDMVAIMDSSTGEMLTWFQHPSGSRNKAGGCEADEHGNVWMTDLNNTAYLLDMGFL
                     LEDMPVWLSADPVEDIVEPGAASQVTLAFDTDRMSVGDVCSTSIAVTSNALDNPLIVV
                     PVTLRVDGLRASFEWREDEPNTVTFTDKSVDEDGGEIVSWLWDFGDGGSSSEQNPVHT
                     YAMNHTYSVTLTVTDSDGAVASDTRQVVLVNAEPVVDFEYRFVEDLAVHFIDKSVDPD
                     GIISSWAWDFGDGTVSSEQHPIHVYSELREYEVTLTVVDNDGAPATIARTMKPDMLPR
                     VTLLAPVGGELWVGKHEIVWIATDVDDPDDTLTIKIEQSSDGGESWMTIADGENNEGL
                     YIWDTTLLNKGGCYIVRITATDPKGGAGTDTSEEFTIIVISRSVVAAPNPAEDNVIFY
                     YDIEADGRLYVYDVAGRLVYEAELPAAANAHHWNLMAGDRPISNGLYLYVLVTETGER
                     SEVGRLVIQR"
     gene            22820..23704
                     /locus_tag="PLU15_07360"
     CDS             22820..23704
                     /locus_tag="PLU15_07360"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOO30778.1"
                     /translation="MQRKISAFLLAVTLVLAMSAAAFAAEDADPIGTAAMLDIGMGAR
                     AMGMGGAHIAVADDAAVIYYNPAGLARIEGRSVTSLYTSLHGAAGYMALGYAQKNVGG
                     GFLRLNASGIEETDEFANVIGTFGMSDFTAMAGYGREVVPGLSLGGTVKLYSQSLPDV
                     SGKGVTGDVGVLYVMDDAKLSFGAVARNLMGKVRYDNIAEDAFERAFGVGAAYRPSDS
                     LLFAADAVFEDGLAARVGAEYRVKQFALRAGGVLDGDETSLTAGAGFAMEGFSVDYAY
                     QTHSVLPDSHRVSLNVRF"
BASE COUNT         5604 a         5767 c         7101 g         5361 t
ORIGIN      
        1 cgcctgcgac atggtggcgg aaggaggtag aggcatgaat atggcggaac tgacagctga
       61 aagcggcaaa gctctaaagg gccttttgga ggcggcaagg ctgaaaccgc acgacatcgt
      121 tgtggtcggc tgcagcacca gcgaggtaat ggggcgcaaa atcgggtcgg catccagcgt
      181 cgatgttgct gaagcgataa tggccgggtt gttgcctgtt atctccgaaa accagttata
      241 cttggccatt caatgctgtg aacatttgaa cagggcctta gtggttgaac aagagtgcgc
      301 agagcgatac cggctggagc gggttacagt cattccccat gtggcagccg gcggggcgct
      361 ggcggcagag gccatgaaga gatttgcaga tcccgtggtc gtggagtcta tctgtgctca
      421 cgcaggtctc gatataggag acacattcat tggcatgcat ttgaaaaggg tagccgtgcc
      481 tgttcgcctt gaagtcacaa agataggcgc cggccacctc acagccgccc gcaccagacc
      541 caagctcata ggaggcgaac gagctcacta tcccacgaga tcgtaggatt gcaggggcat
      601 cgaggcctaa gcaagcctcg ataatttcct gggcgactgc cgggttttca gtctttagcc
      661 gtttcatagg gctgcaggaa ggcaacctcg ggttgaaaca aagagcatca ctgaacctcg
      721 cttcaagggc gcctccctgt gacctgcgaa tttcccgaca tggttttgcc atcacgccag
      781 tgattaccat attactgcaa cgccgtgttc cgcttggcgc gtatagttgc caatggcccg
      841 cattgggtca tttttcgctg catgtacgaa aaagtagacg atgacgggac aacactcttc
      901 atgtccggca gttgagcgga gccaatcgag gctgtggtcg cttcaatccc gaggccacga
      961 gcgtgtacag gctagggtac gctcttgttc tttaagtact gatcaaatcg gttctgctcg
     1021 agaagaaatg acgctacgga gacttgtgga gcacgattaa gccactgtta gttacgaagg
     1081 tgtcctcgtt tttccttggg cccacttatg ggcgttggca gtagccgcta ccaagccgag
     1141 ttgacgcgcc tagggcagga gattcctctg ggggtatgtg ctgctgaaag gctcaatagt
     1201 ggggatctca accatcctca ttctcatgag ttttcgtccg cggaaccatg tcaaaaccaa
     1261 gatatgggcg cagatggtcc caaatggctg gaaacgccgt ggaccactgc cacatttttc
     1321 aaagcccttc cactactatc ccgccatggc agacagattg ccctgattat ggagttagct
     1381 ggctttgaca ttgcacaaaa ggcaagctat aatgcattta atttggggaa tttatgaaat
     1441 actcacttta gttagctgtg caagccaatg agtggaggct tgtagcctgg tatggcgatg
     1501 gtcaaagctg actgcataaa tgatgctatg gtctgggaat tcacaacgtc acacccattt
     1561 gatccttcgg cttgtcttat tcaatgaaca ttaggaacag ggcaatacag caaagcttgc
     1621 aatagtagta ttaataaagc gaattcgtat tacgaatacg catcagcgac tgattgacgt
     1681 ggtagggcaa gggaaactgc gtattccaac ttgctcggag tgcggtagaa cttcagcaat
     1741 actagatgaa atcgtttgag gcacacacgt gaacaggtga cgaatgccaa gttgaactgg
     1801 ggatggcacg ctttgtgggt ggcgggcgca tttcggacca ttgaagttgc cgcaaagtca
     1861 ggtaaagcag tgtgagaaat gcaacgtgtt gaatgttgaa tcaagcagcg tagtgaggct
     1921 ggatactgtc tcatatcggg acttgtctga gcctctttgc attactttga agctgctttg
     1981 cctgattatt agtccacaga agagacgcct ttccaaacag catgtcactt tatctgcttc
     2041 aattgggcgt tcactacttg actgagtgtg cgagtagtga cggagcctgg cctcaatgaa
     2101 agatgttcat agagattcga cagcctatgt cttggcgcag ccatggccgt ttcagcgcgt
     2161 ggacggttga ccgtgcgggc gtgtttgtgc tgccaagcgc tgcattcaga cgcgcggttc
     2221 atgccatagg catagacagt cagacatttg ggcagatggg ggaggtgatg cgtggggcat
     2281 ggattcgaga tgacacatgt caactgtagt agcaacttgc gttatcatgc gcgttgctgc
     2341 gggttggcac gaaagtgatg cctaccggcg gtggcgcgta gatgaggaaa ggggagaaac
     2401 tcatttatgg catgtggcta tagaaagtca ccaaggcttc gagcaacgct actggtggtc
     2461 ttcctgactc tgctcaccgt ggggctggga tcccagggcg cactagggtt cgaagcaaaa
     2521 atgggcggag gatggcagcc tggacccgac gaataccatc ccagccgcct aatagtggaa
     2581 tttgataccg gaatacgaat ccaggcggct gccgattcag ttcataagct cggctatagt
     2641 ctggaaagcg ttcaatcatt tgtgcccaca gagaagtttc cgggcgggct ttctgtcgga
     2701 atagtagata ttcccgaaag cgaaacggtt gaccgggcga ttgctaggtt gagcggggcg
     2761 cctggaatca tccgagctga acgcgactac aaaaagtaca agtgctcgga accagtcttt
     2821 ccaaatgaca tctacttccc gcagatgtgg ccattgcaca accatgatct gcccgaaaat
     2881 tacagagacc cggagatgcc gtggtactat gacccagtca atgacgccga catcgacgcg
     2941 cccgaggcgt gggcgatgtt caaaggatcg agcgaggtaa tagtggctgt aatcgacaca
     3001 ggcacttaca tagaccatcc cgaccttgcc gatcacatct gggtaaacga ggccgagcta
     3061 tacggcgagc caggcgtcga tgacgacggc aacgggtata tcgacgacat acatggatgg
     3121 gacttctaca acaacgacaa ctccgtgttc gacagggatg agcgcgacaa gtggggcgat
     3181 ctcaacgacg aacacggcac ccactgctcc gggaccatag gtgccatgac caacaacggc
     3241 ataggcgttt cggggatcaa ctggaatgtc accatcatgc ctatcaagtt ccttggtccc
     3301 gatggcggat atacctctga ctccatcaag gccatccggt acgccgccgc caacggcgct
     3361 actattgcca gctgcagctg gggcggaggg ggatatgaac agatagtcaa ggacgtcatc
     3421 gaggcgtccg gaatgctctt tttgtgcgcg gcagggaatg aaggcgtcaa tactgacctc
     3481 gaaccgcact atccgtctgg atatgacttg cccaacatca tatccgtagg ggcgagcatg
     3541 cagaatgacg agccttgcta ctattcaggc tggtggggat cgaactacgg ggccaagact
     3601 gttgacctgt ttgccccggg cggattcatc ctttctacgt tgccgccgga tccagtacct
     3661 gatgaaccag ctgagaaata cgcgttcttc tacggaacgt ccatggcgac gccccatgta
     3721 tcaggagtgg ctgcactcgt agccggccag aatcctgata tgccgctcta tccgggcgcg
     3781 cctggatgga aaccagggga tcgcactgtg cgcgacataa tccttggcag tgttgatgtg
     3841 aagtcagcat ttaaaggcaa atgcgtcaca ggaggtagac ttaatgccgc caatgcgctg
     3901 agagccgcaa gcggaccagt gatcgtctcc gcaacagccc agcccgctta cggccaacca
     3961 ccactggacg ttgtcttcag tgcatctgcg actaccccag ttggagtcat tgtggatcag
     4021 tggtgggatt tcggcgatgg ttcggctgtg gttcacgaat tcgatgctca gcacagatac
     4081 tcgacggtcg gcgactacaa tgcctcgttt cacgtagtca atgatttggg cattgaaagc
     4141 gtggcgactg tagagataaa cgtatgcatt cctccggtga tcgagatcaa ccccacttcg
     4201 gtcgagtcca accttgcgtg gggagagact caaacccggc aggtgaccat aacgaacacg
     4261 ggagaaggcg aactgaacta ctccgttgga gtggagctct ttggctcgag ccaggaaggc
     4321 tccgctacag agccccgcgg ttggggaggt cctgacgatt tcggttatat ctggatcgac
     4381 agcgatcatc ctgcggggcc cgagttcgac tggatagaca taagccggat cgggacagca
     4441 cttccgcttc aggatgacgg cggacagacg gtgccgctgc cttttgagtt cccgttctac
     4501 ggaaaggcaa agaccaagat aggcatagcc tccaatgggt atctttcgtt cggaacatgg
     4561 cttagcacat ggaggaccac accaattccg gacagtaccc agcccaacga cttgttggct
     4621 gtatgctgga tcgacttgga tccccaaaga tcaggaatgg tctactactg gggcgacgat
     4681 agcaagttca tcgtggagtg gcaggatgtg cccggaaccc tcgcgggagg gccatacaca
     4741 ttccaggcta tactgacgcc tgacggcgca atcaagtacc agtacaaatc gatgctgggc
     4801 actcacacca acgagtgcac gataggcatc gagaactcca gcggaaccgt cggccttcag
     4861 gtggccaggg acgaactgta cgtccacgac gagttggcca tcttgttcag ccctacctgg
     4921 ataaacgtgt cgcctgtgga ggggactgtg gcgcctggaa gctccgcaat gctcgatgtg
     4981 agattcgatg caggacagct cccgaggggc tcgttccctg cagccatcag ggtgaaaagc
     5041 aacgacccaa tcgctccgga gattgtggtt gacacgtgcc tcaacgtcaa cccgatcatg
     5101 ccgccggtga taacgtctat cgaggcggat cctcggggag gtgcagcgcc tctcgaagtc
     5161 aacttcactg cgtccgccta cgacctgtac ggcgaggtcg tccggattgt gtgggacttc
     5221 ggcgacggca gcgaccctgt cgaaggaacg ctcactgcaa cccacaccta tacagcggaa
     5281 ggggagtacg atgctgtcct gaccgttgta gacgatgacg gccttacggc taccgccacg
     5341 ctccgcatag ctgtgcgcgc gcttccaaag gctgaagtga acccgcctga gataaggcag
     5401 gccatccgtg ctcaccgcac acgaactgaa aggattaccg ttgcgaatac gggagatgct
     5461 gacctagtca ttgaggcctg tgccgccgcg tgcgggtcgc caggcgagga gcccgaacag
     5521 ttcatcaagg aaggctctag cgggcaggac aagttcggct acatatggcg cgactccaac
     5581 atgcccggcg ggcctaagtt cgagtgggca gagatatcgg agatcggtac gaagctgact
     5641 acgctcaaga gtaaccagta cgaagagata gagcttccgt gggaattctc cttctacggg
     5701 cagccgaaga cttctgtcaa ggtgaacgct gcagggtttt taaccttcgg acctactctg
     5761 ggttcgccta cgtccaacac tccgctgccg gatatcggtt tccccaacga gcttcttgct
     5821 gtttggtggg ataccctgag gccggccagt gcgcctcagg acggcggcgt tttccactac
     5881 tatgatgcgg agaatgaccg cgtcatagtg gaatacaaag acgtgccgag gtcgtggaat
     5941 tccgggcaat tgaccttcga ggtcatgctc tatcccgacg gcaggatagt ctaccagtat
     6001 ctcgacatga gcttcggcaa tgatgccgac gaggccagtg ccaccattgg cattgagaac
     6061 gccgatggaa ctgacggact tgaggtgctc tgcgatgctt atggctatat cgaaaacaac
     6121 ctggcgatcg agttcaggac atatcagtgg ctctcggtca gcccgggttc tgctacgatc
     6181 gagccagggc agagtcaggt cttcgatgtg gttatggatc ttgcttctgt gccgcctgtc
     6241 gaccttgacg gctatgtcgt cttcgacaca aacgacgtca gggcgcccag aacgatagtt
     6301 cctgtccacg tcagcgtcat agagaacatt ccgccggtca tctctgcttg tgcagtcaat
     6361 ccgtcgcagg cgccggccgg gacggtattc cagtttgttg cggcagcgga tgatctcgac
     6421 gggacgatag tggacaaata ctggaccttc ggcgacggcg ctcctgtagt tcacgagttt
     6481 actgcggagc acgaatatgt tgaagaagga acatacgttg ccacatttac agcagtggac
     6541 aacgatggtt acaagactag tgtatctata agggtcaggg tgcaggatgc gccgtgcgcg
     6601 tcttggacgc cgggacagtt ccgcttcacg gcggcacaaa gacagactgt ttccgacatt
     6661 ctgattctgc ataacaccgg cgaaggagag ttggtgttcg gaagtgcgcc ggccagcatc
     6721 ctgccacagg cagaagacgt gatgctcccg gagagcaccg aagatgagta cgccatggac
     6781 acccgcgggt ctatcgagcc cgagagggat aatgcccgtt cgccgtggct tcccgagaat
     6841 atcgggagag ttatcaagag ctggacgtgt ccttggccga tgacggctac ctacggtgta
     6901 gcggtcaact acaacactgg cgagctgttc ctcagcgatt cgctgctcaa aaacgacctc
     6961 atcgtcacgc cggacggcaa gtggactgag cgcggttggg cggtgaagtt cccgacaacc
     7021 ttcccggcag acatggcctt tgatggccag tggctgtggc aggttggggt gggatccgga
     7081 aatcccatat acaagatgga tcccgcgacg ggagaagtgc aaggctcgat ctcaaaccga
     7141 gtctggtccg ccaaggccca gcggagcctt gcctacaacc ccaatgatga cacgttctac
     7201 ataggcggtt ggaacgacag aatcatctac catgttaagg gcgaaacttg ggatgtccca
     7261 ggcgaggtgc tggataagtg gccgatgccg gttcccatct cgggacttgc gtaccaccct
     7321 gtggcgaaca ttctcgccgt aactaccaac gcaaaccctg acatggtcta cttcgtggat
     7381 tgcgagagcc atgctaccgt ggcgcagttc ccgcacccat atgcatacta cgcggattat
     7441 acaggcgctg ggtgctcgtt cgacgccgac ggcaatctct ggctggtgtc acaggccaac
     7501 aacagcatgt acctggttga gaccggtctg gggccgatcg ccgctgattg gctcagctgg
     7561 gcgccggaga acggcagggt ggagcccgcg ggttccgcgg ctgtcactgt aaccgtggat
     7621 acgacagagc tcgtgcccgg aacagtgaag ggttctgtaa ttctctatac caacgatgcg
     7681 gaatacccga tgatcatggt tcccgtggtc gccactgtga aggcggcgcc gaggattacg
     7741 gagtgcacgg tgactcctgt gatcggagag cccccgctgg aggtgacctt ccatgcgaaa
     7801 tacgaggtgc ctgaaacccc agtggccagc tatggttggg atttcggaga cggaaccact
     7861 tacgaaggtc tcgatgccgt gcatacatac gctgctgagg gaacctatga ggctgtcttc
     7921 agagtagtgg acgccatcgg cggagtcgat gaagcgagga cgactatcga agtgaagtgg
     7981 ctcccctgcg cgacagtgtc gccacaggag atcaacatta ccctcgctcc taacgaaatg
     8041 gcgtcgcaga cagtcacctt gggtaatgtg gaaggcaatg ctccactcaa ctactcaatc
     8101 aaggtgcagc gtaaaagcgc gcctttcgtg gccatgccgg aagtccttca gtctgtggct
     8161 gatccgtacg cgagaaccgc agcggatctg tccgtgcctt tcgatcagag catcgtggca
     8221 cagatcgcgg aaaacgtgaa accgcaagga gcagggaatc tcgtaacttc atggaccatc
     8281 ccgtctgtgg ccgaagggcc atggggaatc ggagttgaag atcatcacgt atggatctcc
     8341 gatactcgat tgattgcgga ccacaagatg acaaaagacg gagaatatac tggcgtgtcc
     8401 tttgaaacgc cgtggattgg gtttggagga tggcctgccg acatggcatt tgatacagtg
     8461 cgcaacatca tgtggcaggt gaacaccgct ggcgatctag gcatatacgg gattgatccg
     8521 gccactggcg aagtggtgga caggatcatc tccaatgagc tttgggccaa gatgtcgcag
     8581 cggggcctcg cctacaatcc cgatgacgac acgttctaca tcggtggttg gaaccagaag
     8641 atcatcttcc acatcaaggg accgagctgg gatcagcccg gagcaatact ggaacagtgg
     8701 tcgttccctg tgaacatagc agggcttgca tatcacccag caggggtcct gtgggtgacc
     8761 gacaatggac agcccgacat gatctacggt atcgacgtgg aaactcacaa ggttatcgag
     8821 cagttcctcc acccggctta cctgtacaac agcggtgccg gagcagaagt ggacagtgaa
     8881 ggaaacctct gggtaatctc gatggatgag aagatggtat acgtcgtgag caccggattg
     8941 ccgccgatgc ctccgatagg cgtcgaccct gggtcaggga gtatcccggc gggcgagaca
     9001 tgcgagatca ccgtcgagtt tgatgctcta aagcttggcc gtcccggtga cgatgtcgaa
     9061 agatatctgg aaatcgtaac tgacgatcct atggttcaat ctctcaacgt gaaacttaac
     9121 atccatatcg agggaggacc ggtgatcagc aaagtcacgg ctacccctgc catcggccag
     9181 ccacctcttg ccgtcgtatt cgacggtctg gttgagccgg gcgatgtgcc gattacggat
     9241 gtatggtggg atttcggcga cggttccgag tctgtacacg atgcccatgc cgagcacaca
     9301 tacaccagcg taggccagta cgaggccgag cttcacgcgg tggatgaaaa tggcgtaagc
     9361 gcgtcggaga aggtcacagt cacggtcaaa tggctgcctg tgttcaatgt cgagccgatg
     9421 agcttcgacg aggtcgtgct cgccgggact gaggctcagt cgctgctgac catatccaac
     9481 accggagtct cagcgatgga ctttgcagtc acaaccatgc cgagcttcgc ccagagccct
     9541 gagtggaagc ggtatgcgga gatggagcct gtgaagggcg agtcggcctc taggccaata
     9601 gggtttgcag gcgccggcgc cggcgggccc gaccagttcg ggtatgtatg gatggacagc
     9661 aatcaggaga acggaccggt gttcgactgg ttcgagataa gagaggtcgg aaggagaatc
     9721 gagctcttcg acgaagatgg cgtcacagtg cctcttccgt tcaagttccc gttctacggc
     9781 agcttgaaga cgcaggtggg gatagcttca aatggttatc tcagcttcga ccagagccag
     9841 ttgaaggggc atttcaggaa tagcccgatt ccgaattcgg cagatccaaa cgatctgatg
     9901 gcggtctact ggaccgacct tgatcctgag gcgttcgggg atgtctacta ctaccatgat
     9961 gaactgaacg gaaggttcat agtggaatac gacaatgtgc ctgagtgggg cgatgagata
    10021 ggatctactt accaggtaat cctctatccc aacggcacga tagtctatca gtatctatcg
    10081 atcaatggcg atccagaggc tgttacggtc ggtattgaag atgccatcgg tgctgacggg
    10141 ttgcaggtag tgtacaacgc tgcatacttg gaggacgggc ttgccgtggc cttctctccc
    10201 gcaggttccg tcgtgagagt caaccccgac tccgggcatt tagtgccagg cggaaagcag
    10261 gatgtcgttg tgacgctcgg ttcgcctgat gcggcgcccg gaacatactc gctctatctg
    10321 tacgtgacgt cagatgatcc gttccgtccg tgcgcaacta tccctgtgac ggtcaagctc
    10381 aaccaggcgc caacggtgac gattgtctct ccggcggcga gcgacaagtg ggaaggaacg
    10441 aaggagatcg tctggaaggc agcggatccg gatgatcctg cagattcgct gtcagtcgat
    10501 atagcatgga cccgtgatgg agagacgtgg aacgagatcg cttcggctct gccgaactca
    10561 ggttcctacg agtgggacac aaaggaagtc ggcaagagcg gcgacagctt ccgtgtgcga
    10621 atcgttgttt cagatcctgc gggagaaagc gcagaggcgg taagcgggga gtttgccatc
    10681 gaggtgctca acgtggcgcc tgaggtgaag atcctcaagc ctgaagccgg tgcggtctgg
    10741 acagggacac gggcgatcga gtatgaagct gtcgacgcag acggggatac tctcgccatc
    10801 ttgctggagt atgactgtct cgacgatgag gctggctggc agttgatagc tgatggagag
    10861 gagaacacag gcaagtatct gtgggacatc tcaaaactgg ccaagggcgg gttgtacaag
    10921 gtgcgggtga ccgcaaccga cccggatggg gccgagggac atgacgtcag tgatgaattc
    10981 acagtcatcg ttctcgccca cgcagttgtt gcagcgccca acccggccag gcagtcagtg
    11041 acgttctact atgacgccga aacagatggg aagctctacg tgtacgatgt ggccggacgg
    11101 cttgtttaca gtgcagagct gaccgcggga tccaccttcc atatgtggga tctcactttg
    11161 ggcggcaggc cggtcgccag cggactgtac ctctacgtcg ttgtgaccga tggcggtgaa
    11221 acatctgagg ttggacggct ggtcatacaa aggtagacaa ctcaaaaagc aatgaatgtg
    11281 cctcttagag gcggggcgca gggcgggttt ccctgcgcct cctctgaggg gtaagcaaag
    11341 caccaggtca tgaaagcaaa ggaggaccag ctgatgcaac gcaagataag cgcgtttctg
    11401 ttggcggtaa cattggtgtt ggccatgtcg gctgcggctt ttgccgcaga agatgccgat
    11461 cctatcggca ccgctgcaat gcttgacata gggatgggcg ctcgggccat ggggatgggc
    11521 ggtgcccata tcgccgtggc cgatgacgca gccgtgatct actacaaccc cgcagggctt
    11581 gcaaggatag agggacggag cgtgacttcg ctctacacga gcttgcacgg ggctgccggg
    11641 tacatggcct taggatacgc tcagaagaac gtgggcgggg gattcctcag gctcaatgct
    11701 tcggggattg aagagactga tgaatttgca aacgtgatcg gaacctttgg aatgagtgat
    11761 ttcacggcga tggccggata cggacgcgaa gtcgtgccag ggctcagcct tggcggaacg
    11821 gtgaagctct atagccagtc gctgcctgat gtatcaggca aaggtgttac aggagatgtc
    11881 ggagtgctat acgtgatgga cgatgccaag ctgagcttcg gcgctgttgc caggaacctc
    11941 atgggcaaag tgcggtacga caatatagct gaagatgcct tcgaacgagc ctttggcgta
    12001 ggcgcagcct accgtccttc ggatagcctt ctgtttgctg ctgatgcagt gttcgaggat
    12061 ggccttgcgg caagggtcgg ggctgaatac cgcgtcaagc agtttgccct cagagccggc
    12121 ggagtgctgg acggggatga gacatcgctc acggcgggcg cagggtttgc catggagggc
    12181 ttctccgtgg actatgcgta ccagacccat agcgtgctcc ctgattcgca cagggtttcg
    12241 ctgaatgtga ggttttaact aggtgaagag tgtcagccgg gccggccaat cgggccgtgt
    12301 tcggcagcaa ggcccgcagc gcggaagatg aaggcgttgc gggccttgaa ttagttcaag
    12361 ttctgcactt tcacaagcaa tgccaagctc atataaggtt acgttctaga attggtcact
    12421 taccatgatt tcatggagca catcaaaggc cttgtagatt ctcagggttt ctttgtgggc
    12481 agttgggaga aggaccgtga ctcgctccac gagtcattgt tacggaaggg cttgtcgatt
    12541 gctatgagaa ggtgttgcgc aagcactgca gagctgaagg atttaagagc ctcggacgag
    12601 aaattaaaat tatttgaacc atttcggcgt ctggagttgg taaccgcgga ttgtttccga
    12661 gattgctttt gctggttgct ggaggtgtgc gccacgtggg atacgcgatt gtaggggttt
    12721 tgatcgtctc tggaatcatt ctgttgggca tcagtatcta tgtggcgatt gaaggcagaa
    12781 gaagaaggaa tctgtggttt gcaggattcg ctctcgccgc tgccctgcat tcgtttgggt
    12841 atgcattcaa agtgactgcc aacagtgtag aggaggcact tgtctacata aggatcgcgt
    12901 acttgggcat ctcttttctt cctaccttcg gttttttagc cgctttggag cttgcaggaa
    12961 aggataggca tctcaagaag ggcacgatct tgagcatgat agcattttcc gcagtcactt
    13021 tggcgattgt atgcaccaac agcttgcacc atctgtacta tattgacatc gtactcacca
    13081 aggcgaatgt gcttacgata gttcacgttg tgcctggcat atggtacaag gttttcatga
    13141 cgtacgtcaa cctagctttt gctgcgggca tcgtgatcat ggctagagcc ctagcggagg
    13201 caccggtaga tctgaaaccc caatactggg tttacttcgt cagctttctc gttcccggag
    13261 tggggaacct tgtttatctt cttgggctca gcccatatga tgcatacctg gctccgttct
    13321 acttaatgtt catgagcgtg ctcctgctct ttggtttgaa tgagaatcaa cttttccagg
    13381 ttgtgagcat tgccaagagc agagtacttg acagtatgga tgatgctgtt ctaatcatca
    13441 acagaaacaa tcgcattatc gaccataacc attccttgac gcatattgtg gcaggcaatt
    13501 cggacgatta cgtcggggag cgcctgtctg acgtcttcag ttcccaccct caggtagtcg
    13561 attttgtgac gaaccatgca aatggccaga ccaatgtggc tcttcaagat ggttccggcc
    13621 gcgtgtacag agtcagagtc cgcacggtgc agggcaggag aaagggcgca tttgtgaagt
    13681 gtctgactat gagcgacatg actcgagaga tgaacgtgat gagggactta aaagaccttg
    13741 ctcatcgtga tgccctcact aatattgcga acagaagggc gctctactgc caggcggaag
    13801 atgtattgag gcggtgcggg actggcatgg ccgtctcttc aatcatgttg gatatcgacg
    13861 acttcaagca ggttaatgat agctacggac atgaggcggg agacatggtc ctgaaggaag
    13921 tggctttgct tctgaagcag agtatacggg ctcaggatat tttggctcgt tacgggggcg
    13981 aggagtttgt gttgataatg ccggacaccg aagtggatgc cgcctttgcg ctggcggaaa
    14041 ggttgcgaaa gaagatagct ggcgctgaga tattctacaa tgatcagagg attgagctta
    14101 ctgtcagcat gggggtagca ggcgaaatct gctcgccaga tctgcaagtc gatcagctta
    14161 tcaagaatgc agatgccgcg ctttatgagg ccaagcgctc cggacggaac aaagttgcgc
    14221 tctatcgtga ttgtgatgga taaagccggt gttctgccat gatggcattg atcttgccag
    14281 cgatttgggg tgagaggcat atgatgtcct cgccttcgag atagtgtacc actgggtctg
    14341 tatggttgtg agaaggagga aggccaaatc gcagcattga gcgaagacgc gcgttgccat
    14401 ccgccgcctg ggccgacatc cccaaaactg cgattgacaa ttgttctggg tatggttagc
    14461 tcccagatga tccaaccata ggcgtcaaac ccatacaatg cttcactgca tcctaaggcc
    14521 tggtaaaagg tgcatcaacc ccacattgtt gtgaggcaat ggtgaccgat ttcaaaggga
    14581 ttccagattg ttctgacgaa tagattagag aaatgcctta tctgggggaa gccccgagct
    14641 tctccgatca tgcaggccat gccataagaa gtgggagggc ttcgacgggt cgacatggga
    14701 ctgtttgcta tgactgaaat gcgcaacata ccgacgcact acctaatgga ccagatgttg
    14761 ttggcttcaa catcgcaata ttgatctagc acattcgccc acattgcctg ctttcgccga
    14821 gctagggaac ttgcagcaga catgttgcat catcattaca gctgactggg gtgtgatgag
    14881 catgcacaag gactgcgact aaagatagat tccaagggtg gttgcgtgat gtcattgtgg
    14941 gacagatcga tgtgcgagtt tatgcagcaa gtagctgagg cagtgtctac tgttttgcag
    15001 ttggatgtca ctatagttga acgcgggttg acgcgggttg ccggcactgg acgatactcc
    15061 agacttgttg gtcagaaggt tgcctcgtcg tccgtttttg ccgagtcaat gcgaactggc
    15121 aaacctcgct tgatcacgga cccgaggcgg aacgtcgcct gcgggaagtg cgaggtcgca
    15181 gagaaatgta tcgagacatg tcacatggtt tatcctctca tgatggatgg cgaaccttta
    15241 ggggtggtag gcctcatatg cttcacggaa gatcagcgcc ggcgtgttgt gaactgttct
    15301 gacgagtatg tggcatttat ggaacagatg gccaggctcg ttgaaagcgg tgcgaagaat
    15361 gcctacgtta cacaagaagt caagaaatct cgtgaccagc ttactggtat agtcaatgct
    15421 gttgatgaag gcataattgc agtagacgac gacggaattg ttctctgctg caatcgagca
    15481 gcatcaaagg tgcttggctt agacggagtc gagcttgtag gaaaaaaact cggtgacgtg
    15541 gttgctgatg cacccatact ggaagttgtg caaacccgga cgccatataa agggaaagag
    15601 gtcatagtct cggtcccggc ggggcgaatc cagtatgtca gcagtggatc agttctgcgc
    15661 aacggaggtt gtgtcgtggg cgctgtggag ttgctcacca gctccaaaaa tgcaagacgt
    15721 tttgcatacc aagtttcaaa tagtcagcca gaccagccca tcgacaatat acttggcacg
    15781 gcgcctgcac tcatggaggc taagaagatg gccctcagta cggcggccag cttctccaca
    15841 gtcttgctga ttggcgagag cggcacaggt aaggaactct ttgctcgcgc tattcattat
    15901 cacagcgctc gacggtatgg accatttgtc acagtgaatt gtgctgccat gcccgaggag
    15961 ctgctcgatt cggagctgtt tggttacgaa gaaggtgcgt ttaccggcgc ccgcaaaggg
    16021 ggcaagcccg gcaagtttga gctcgcagac gaaggaacct tgttcctcga cgaggttgca
    16081 gattgctcgc tgaggcttca ggcgaaactc cttcgtgcac tggacgatgg agagatttgt
    16141 cgtatcggtg gaacacagac tctgacttca aatgcgcgaa tcattgctgc aaccaataga
    16201 aatctcgaac aaatggttcg acagcgcgag ttccgagagg acttgtattt ccgcttgaac
    16261 gttattccca tccgcattcc gcctctaaga gaacgtaaga ccgatattat tcccctcttc
    16321 agttatttcc tgaacaagca tgctatttta tcgcagcggg acattcctgt gctttgcgag
    16381 gaggtcgagc agattctgct tgtctatgat tggccgggca acataaggca attggaaaac
    16441 gcgactcaat atgttctcca tacgtgccta ggacacactg tagaacctca ccacctcccg
    16501 gcacaggtga ccaaaggcac aatggaacat ggaggctcgt cgactggtga catagccaat
    16561 gaaacaggaa gtcaggcctt caactggttc gtatcaaaat cggaatggga acggatggct
    16621 atagccgagg gcttgaggca gctagggcat agtggcaaag caaaggagac tatcgcggaa
    16681 cgactgggta tcagtcgtgc cactgtatat agaaggatcc gtgagtatga tctgggcgga
    16741 taactgtcaa gcataaagta gccagctatc tcccgttaat tgtctgcctg ttttgttgcc
    16801 ttagcgtggt ctcgaggtga gaccaatctc agattgagat tagggcacgt gccatccccc
    16861 tctggcttct gccagcggat acgtgttgat caaatcatgg cacagatatt gcaaataaat
    16921 ctgaggtact ggtttgtgat tattatctga atgaagtgga gggagattag tggccaaggc
    16981 taggcgccca aattgggcaa gtatggcaat tatcatggtc atcgttgtat tctttgcggc
    17041 ttcggtcgag tcggcatcta cagctagccc ggtagatgct gggaatgcct gggcccagca
    17101 ggaactggct ggggaggcgt ttgaggatgg cgaatatctg gttagattca ttgacggtgt
    17161 aggttcttcg gaagttacgg gcagcttgga ccgtatcggg atgaagattg taaagccgat
    17221 atggttcaag ccgagtgatg catttcctca aggactgact atttatcggg tgaaggtctg
    17281 tgatggccag agaatcaatg cggaaagcgt ggtcgaaaaa ctcggggcaa acccgcaagt
    17341 aatgtacgta tcgccacaga gaaaggttta tgcgtacgat tcgccatcag cagtgatccc
    17401 ggacgatccc ctgtttccca ggatgtgggg cctgaacaat accggtcaag aatcgtaccc
    17461 ggggctcgtc ggtactcctg atgctgacat agatgcgcct gaggcgtggg ccatcaggca
    17521 cgatgtgcca gaaatcgttg ttgccgttct cgataccggg atagagtggg atcatccgga
    17581 tcttatggat aatatatgga ttaattccgg ggagatcccg ggcaatggtg ccgatgatga
    17641 cggaaacggg tatattgatg atatttatgg atgggatttc gcaaacgatg acaacagcgt
    17701 gtatgatggc ttggattcac atggaacgca taccgcggga accataggcg ctgtaggcaa
    17761 caatggcctt ggagtgaccg gaattgcctg gaataccaag attatgtgtt tgaagtttct
    17821 tgaagatggt gtcggggaca ctggtaatgc tattgaagct ctagcgtatg cttggaacaa
    17881 cggagcgcat atcacctcga attcctgggg gtacagcggg cccctcgatc cagcgctcga
    17941 ggatgccttc agggcctgtg atttgctcca tatatgcgct gcgggaaata ctgggagcga
    18001 taatgattct aaatatccaa aacaaagcca ctacccgtcc agttttccac tggataacat
    18061 aatcgcggtc gccgcaaccg actgcaatga taggctggcg gctttttcgt gttggggaaa
    18121 gaccacagtg gatattgccg ctccgggcca ccgtattctt tcgacactgc ctacagacca
    18181 aaccccggag ggatatctgc catatgcatg gatgaatggc acatccatgg cgactccaca
    18241 cgttgcggga gcctgcgcag tgctaatggc cgaatttccg gatatgcctc tttacccttg
    18301 gggtagtgat tggcaggatg accaggtgac aataaaagaa ctcattctgt cgactgctga
    18361 caagaagccc gatctcgaag gcgtagttct atctggtggg cggctaaaca tctgcaacgc
    18421 cctgcgacag caggttccgc cgaagatcgc taatgtaagt ggttttcctc cctatgggag
    18481 ccctccattg gcggttacct ttgcggctac ggtggaagat gaagcgaata ttttggatgc
    18541 gtggtgggat ttcggggatg gcagcgaagc ggtgcatgca tatacggcca gtcatgtata
    18601 tggtcagacg ggcatgtata cttcaacttt ctacgccata gggagaaatg gtattggggc
    18661 aaatgcgagc gtgagggtta ccgtgtatga cgagggctcc gtcatctttg tggacgatga
    18721 tggtggggcg agcgatagcg aggtattcct caatgctttg aatggcattg gagtgccatg
    18781 ggtattagtg gaaccacctg tgccattttc ccctgatatc agcaatcctg tgatatggac
    18841 caccggcaga acaatgcagc ggacattgac ctccgaggat caaatctggc tttcgcagta
    18901 tcttgataac ggcggcagat tattcctatg cggtcaggac atcatctggg acttaggtcg
    18961 tgataatgcc ttcgtccagc agtacctaca cgtggccgag tacgtgaagg atgttagaac
    19021 agacaacgtg tacggcattc cggatgatcc gctcactgat gggatggtta tcaagctgga
    19081 ttacccgttc aaagactaca gcgacacagt acttcctgat aaatacgcaa ccccgatatt
    19141 cagtaacgat tcggggcgcc cctgtgcgct gaagtactat ggaccggata cgtacaggtt
    19201 ggttttcttc gtcttcccgt ttgaggcgat tcctgatatc aaatggaccg acggagagaa
    19261 cgcagacacc gggattgagg cgactgcgac agacgtcatg gcggcaatat atgagtttat
    19321 ctctgaagag ggagatccga aggcgtctgt ttgccccgag tctttcgata taaccctcgc
    19381 ccgcgggtca tcagcgtctg aggtccttgc catatccaat acgggagaat cgaggttctt
    19441 cttcgagctt ggcattgacc gatcggacgc tggcaatccg ggcggcgatg ggccattcgg
    19501 cagacgcgat acgtatggct acatatggag agatagcgat agcccggagg gacccgagtt
    19561 caaatgggtt gacatcacgg atcggggtac cctcggcgta ctgggtgatg attactccat
    19621 agatatcgag ttgccgttcg aattcatgtt ttatggcgaa gcgaagaata gggttacgat
    19681 ttccgctgat gggttcctca cttttggcgt cacaggttcc gcagctgtga acacggccct
    19741 gcccgatcgt gcccttccca atgatctaat agctgttttc tgggatgatc tgcaggttac
    19801 tgatggcggg gtctattact attacgacgc tgcaaatgaa agattcattg tgtcgtgggt
    19861 gggcgcccat cgcatcgaaa acaaggtccc agaaggttgc ttcaacttcc aggcaatact
    19921 gtaccgcaac gggaagattg tgtaccagta tgccagcatg gatggaacca tgaccaatgc
    19981 cacagtgggc attgaagaca gatctggcga agtgggcctc caagtcatct gcaatgccga
    20041 gtacctccat agcaacatgg cagtggagct gatccacgag gcagatggca tttcgttcgg
    20101 tagaaggatt ggcgcggtga ggcccggtga gatgatcgac atagaagtag tcatcgacgc
    20161 ccagatgcta gatgaaggca aatacgcgcg gcagatattg gtgaacatcg acgatcctgt
    20221 caacccggtc atagccgtgc cggtgaacat cgcagtgaca ggcaacgagt tgcctgttgt
    20281 aacaaaggcc acggtcactc ccgatcatgg cgaaccgccc ctaacggtga tgcttgaagc
    20341 gggagcgtat gactcagatg gagaactggc cgatttgtgg tgggattttg gagatgggac
    20401 ccctcccgag cacgatctcc agaccactca tgtttatgag aatcctggat tctatgaagc
    20461 ctccttccat gccgtggaca acctgggagc aagcacttca gttacagtgc cagtcagggt
    20521 tgagggggct gcttatgcca gtgtcaaacc tgatgctctg gtcgtttcag taatcccaca
    20581 cagcacagcg acacgtcgac tggaaataca aaatagcggg acttcgccat tgagattcat
    20641 ggtgacgggt tttaagacga acgaagccaa tttcacgggc tacgaaagaa aggcagtttt
    20701 ggatggttgg gatccgaatg cgtatgattc tggggatcag tttgttattg aagacagaac
    20761 catcacaaaa ctggatattg gcgataaggt gcaatcatgg ccgataaatg gagtattcag
    20821 agcatggggt tgtggatacg atggggagaa cgtgtggttc actgatctca gaaagcgcga
    20881 ttggcaattc actaccgagg gagagaagac aggcgtaact ttcgagttgc cctgggtagg
    20941 acaatgggcg gccgacctcg cttgggatgg agaatatctc tggcacgtca atgtgggagg
    21001 cgataacagg atatactgca tagaccctgc cgatggcgaa gtggtcacat caataaggga
    21061 tccctacggt atttgggatg ccacctccca gcggggaatt gcatacgatc ctgtggagga
    21121 cgtgttctac atcggcgggt ggaaccagct ggtaatctac aaaattgcgg ggctttcatg
    21181 gcccagaccc ggtgagattc ttcggacgta tgacgatagt actctgccgg tggcaggcat
    21241 cgcacgagtc ggcaggtact tggtcttgac gctaaatggt cgaccggaca tggtggccat
    21301 catggattcc agcactggtg aaatgctcac ttggtttcag catccgagtg gcagcaggaa
    21361 caaagctggc ggctgcgagg cagacgagca cggcaacgtt tggatgactg atctaaacaa
    21421 tacggcatat ctcctggata tgggtttctt actagaagac atgcccgtgt ggctgagcgc
    21481 cgatccggtc gaagatattg tggaacccgg agcagcttca caagttactc tggcatttga
    21541 cacagataga atgagtgtgg gcgatgtttg cagcacgagc attgccgtaa ccagcaacgc
    21601 tttggacaat cccctcatag tggttcctgt gaccttacga gtggacggac tgagggcgag
    21661 ttttgaatgg cgcgaagatg aacccaatac ggttacgttc acagacaaat cagtggatga
    21721 agatggcggg gagatcgtca gttggctatg ggattttgga gacggcggat cttcgtctga
    21781 acagaatccg gttcatacct atgcgatgaa ccacacgtac tctgtaaccc tcacagtgac
    21841 agattccgat ggagcagtgg ccagtgatac tcggcaggtt gttttggtca atgctgaacc
    21901 ggtggtcgat tttgaataca ggttcgtgga agacttagcg gttcatttca tcgacaaatc
    21961 agtcgatccg gacgggataa taagcagctg ggcatgggat tttggcgatg ggactgtgtc
    22021 ttcagagcaa catcccatcc atgtttatag tgagcttaga gaatacgaag tcacccttac
    22081 cgtggtcgat aatgatggag cgccagctac catcgcaagg accatgaagc cggacatgct
    22141 tccacgggtt acactgcttg cgcccgttgg aggagaatta tgggtgggca agcacgagat
    22201 cgtatggata gctactgatg tagatgatcc tgatgatact ctcacaatca agatcgaaca
    22261 gtcttctgac ggcggcgaat catggatgac aatcgctgat ggagagaaca acgagggctt
    22321 atacatatgg gacaccacac ttttgaataa gggtgggtgc tatattgtgc ggattactgc
    22381 tactgatccc aagggcggag caggaaccga tacaagtgag gagttcacaa tcattgtgat
    22441 atcgcggtct gttgtggctg cgccaaaccc ggcagaagac aatgtgatct tctattacga
    22501 tatcgaggca gatggaaggc tatacgtata tgacgtagcc ggaagactcg tctatgaggc
    22561 agaattgcca gcagccgcta acgcgcacca ttggaacctc atggcggggg ataggccaat
    22621 ttccaatggg ctgtatctct atgtgttggt gactgagaca ggcgagaggt ccgaagtggg
    22681 acggctggtc atacagaggt agacaactca aaaagcaatg aatgtgcctc ttagaggcgg
    22741 ggcgcagggc gggtttccct gcgcctcctc tgaggggtaa gcaaagcacc aggtcatgaa
    22801 agcaaaggag gaccagctga tgcaacgcaa gataagcgcg tttctgttgg cggtaacatt
    22861 ggtgttggcc atgtcggctg cggcttttgc cgcagaagat gccgatccta tcggcaccgc
    22921 tgcaatgctt gacataggga tgggcgctcg ggccatgggg atgggcggtg cccatatcgc
    22981 cgtggccgat gacgcagccg tgatctacta caaccccgca gggcttgcaa ggatagaggg
    23041 acggagcgtg acttcgctct acacgagctt gcacggggct gccgggtaca tggccttagg
    23101 atacgctcag aagaacgtgg gcgggggatt cctcaggctc aatgcttcgg ggattgaaga
    23161 gactgatgaa tttgcaaacg tgatcggaac ctttggaatg agtgatttca cggcgatggc
    23221 cggatacgga cgcgaagtcg tgccagggct cagccttggc ggaacggtga agctctatag
    23281 ccagtcgctg cctgatgtat caggcaaagg tgttacagga gatgtcggag tgctatacgt
    23341 gatggacgat gccaagctga gcttcggcgc tgttgccagg aacctcatgg gcaaagtgcg
    23401 gtacgacaat atagctgaag atgccttcga acgagccttt ggcgtaggcg cagcctaccg
    23461 tccttcggat agccttctgt ttgctgctga tgcagtgttc gaggatggcc ttgcggcaag
    23521 ggtcggggct gaataccgcg tcaagcagtt tgccctcaga gccggcggag tgctggacgg
    23581 ggatgagaca tcgctcacgg cgggcgcagg gtttgccatg gagggcttct ccgtggacta
    23641 tgcgtaccag acccatagcg tgctccctga ttcgcacagg gtttcgctga atgtgaggtt
    23701 ttaactaggt gaagagtgtc agccgggccg gccaatcggg ccgtgttcgg cagcaaggcc
    23761 cgcagcgcgg aagatgaagg cgttgcgggc ctctttctgc tctgtgctag gcgatagtgt
    23821 tgcccggcat gct
//
LOCUS       DAOSYL010000040        23316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1811_length_23316_cov_8.130863, whole genome shotgun sequence.
ACCESSION   DAOSYL010000040 DAOSYL010000000
VERSION     DAOSYL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23316
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1811_length_23316_cov_8.130863"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1054)
                     /locus_tag="PLU15_07365"
     CDS             complement(143..1054)
                     /locus_tag="PLU15_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOO30779.1"
                     /translation="MARKNEFGGSWWAKRWTFALESFGWANRLQRGRSYARAGNVLSV
                     DVSPGMAEAKVQGSRSRPYKVQIRMKPLNESDWDRVTTAMAESAAFAASLLAGEMPEN
                     IEEAFAQCDAALFPVSEEDLATSCTCPDWANPCKHIAAVHYVLAQEFDRDPFLLFLLR
                     GMRKEDLLISLRRKRSQAACQEASEPENTTRCTDAAAALAEDLCPSPEEFWNGDVEHL
                     DHVAISIAPPSIDAVAIKHLGPLPLRDQGNTNLSRTFIQTMDHYYKAVTKQAITWAYG
                     DDQEGSTAFDCNSHKDNGTVHKKSLQR"
     gene            complement(1106..4456)
                     /locus_tag="PLU15_07370"
     CDS             complement(1106..4456)
                     /locus_tag="PLU15_07370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO30780.1"
                     /translation="MIHATWLPSSSQAPGKVSTSTAISDARALGGVFFIWAEVPQRYG
                     HKARGRQGVYKQHPFQATRAELAEAKLVLSAIATGTSGAQDSERLTSAPPEFRRALLL
                     LPSVCGYPQPSPDLGSQTESLEEPDPFGQKAHATFELFQVNGIAIAAPDAVKIFSALP
                     DSARILPCSLQGQHPVMLGADVRYWSLAAKLVLELVARQRFLPGICQAAGARASERKQ
                     CSSFRGVWLPVLDEQTDILRINALAHAMPGISRAVLPHTKARSGEAGMTLAQATDSIL
                     CKEDLLQSFICTAMDETVRAILENERSEMISRAQALGQRGDEAENRLTMELLGVVSPT
                     HSWIANLAREDSRISGDKSDVRSLFRAVKEWAAQAETPGPQGGFRTCFKLETPEDPED
                     PWTLRYLLQAVDDPSVLVPAEKVWEESGGVLRFLGRRFENPQERLLADLARVGSFIPQ
                     VEESLKDPCPESCQLSPAEAYSFLRDICPLLQEGGYGTLVPPWWDRTSGNAANVGITL
                     KVGGQAASSRLTASTLVDYDWKVAIGDQEMSSEEFDRLASLKVPLVKVRGEWVVIRPD
                     DVEAAIRFWNNPKRRQQMSVQEILSLSLGMGEEIEAQNGLPVTAIEADGWMGELLDRL
                     NSGEKISELPQPEGFVGNLRPYQIRGYSWLAFLKQWGLGACLADDMGLGKTIQIIALL
                     LYEKDPGPSLLVCPTSVAGNWKRELARFAPSLNVMLHHGSDRLIGDEFAQEAKQSDVV
                     ITTYALLSRDEDTLSSLHWNSLVLDEAQNIKNHGTKQAQSARRLTADFRVALTGTPVE
                     NRLFELWSIMDFLNPGYLGSAAGFRQHFATPIERYGDAAASARLRKLTAPFILRRVKT
                     DPLIIQDLPEKHETKVYCNLTKEQATLYEAVVRDMIATIEEASGMERRGAILAALLKL
                     KQVCNHPAQFLHDNSRLEGRSGKLSRLEEMLDEIVEEGDRALIFTQFSEMGTLLKHYL
                     SKALRQEVLFLHGGVPQKERDRMIERFQNDPNGPPIFILSLKAGGLGLNLTQASYVFH
                     FDRWWNPAVEDQATDRAFRIGQKKNVNVYKFLCGGTVEERIDQMIDEKRALADSIIGT
                     GEEWITEMSTDELRNIFTLRDESIIDDD"
     gene            complement(4653..5915)
                     /locus_tag="PLU15_07375"
     CDS             complement(4653..5915)
                     /locus_tag="PLU15_07375"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOO30781.1"
                     /translation="MKLTLVGAGSTYTPELVDGLIRRRQDLPVETLSLMDIDKERLTT
                     VGSFVKRMVDASGESLNIEMHTQSCDAIVGADFVVTQIRVGGQLWRDLDCKLAMEHGL
                     IGQETTGFAGFAKAIRTIPPILEICKTMREAAPDAWLINFTNPSGIITEAIARHGRVR
                     AIGLCNVPINMKLEAARALGVPANEISLDYIGLNHLSWIRKVHWLGRDVTDQALAIAA
                     SSMQKNISGTPATPKLAEALSMVPSPYLKYFYRPDASIAEQKSSPKTRAEEVMEIEAE
                     LMRIYKDPAVVSKPPALAKRGGAHYSTVSIDIMSSVYNDSGKVEIVDIPHAGAVDGFP
                     ASSVMEIPAVIDATGAHPIHIGSIEPEIRGLMHQVKAYEELTIEAAVTHSYNRALLAL
                     INNPLVQDAGKAERLLDALVESKRASLR"
     gene            complement(5912..6940)
                     /locus_tag="PLU15_07380"
     CDS             complement(5912..6940)
                     /locus_tag="PLU15_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOO30782.1"
                     /translation="MSTKDSPYVIGVDGGGTKTLAVAATLAGEIIAAARSGPANYQSA
                     GKDVAASNIRQVISEIAEITSAPRGCLAVYYALSGADRPLDFEIIEDMIGGITPSPPW
                     PHWEVDNDALAPLALGSEDGTGVVVICGTGTNCIGINATGQRKQVGGLSPAFGDAAGG
                     SVIGIRAYSAAIRGEDGRGRPTLLSQLIRSHIGSSDLLSVVHHTYADGEGFAFSSIAP
                     LVFEAAERGDSVAVEILIDNGRELGISALAALRELFSPEDDPTVVLAGSIAQNPNPIT
                     LTAVREAVLPEYPNARIMVPTAEPVLGAVLLATRLAGKGLAVESSREFMKRLEHSYST
                     FDRSESLQ"
     gene            7482..8513
                     /locus_tag="PLU15_07385"
     CDS             7482..8513
                     /locus_tag="PLU15_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO30783.1"
                     /translation="MRKWWVAISTIYEVAQKAGVSVATASRALNDSGYVSRESRDRVL
                     AAAKALDYHINVNARSLTSGKTYTIALVLPDVTNPFFPAVARGVEDYASDNGYCVILC
                     NTDGNARKESDYVRMLRSRKVDGVIFAVSVASKSRVSALTDDGIAVVLVDRDLGDSCD
                     IVKTDNIAGALLATNHLIGLGHTSIAFISGPMSLATSQERFRGYHDALVSGGVSFDER
                     LVVEGDFRMKSGYEAMRDLLRKPCQFTAVFAANDLMAVGAMTALEESGFHVPTDVAVM
                     GYDDIDIASVTRPRLSTVAQPKYEMGWIAAETVIRRMCNPKASRMEELLQPMLVVRDS
                     TVERRGASV"
     gene            8510..9505
                     /gene="rbsK"
                     /locus_tag="PLU15_07390"
     CDS             8510..9505
                     /gene="rbsK"
                     /locus_tag="PLU15_07390"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOO30784.1"
                     /translation="MSIVVVGSCNMDYMMQVTSLPKRGETIFAVSHSTAAGGKGANQA
                     VAAARLGSRVYMVGRVGSDEAGRVLVESLEMAGVDASKVGKDVMESTGSAYITVDRDG
                     ANTIVVNRGANFAIKPSHVTAAESLIQEADVVVVQLETPIEVVFQALRLARKHGAVTV
                     LNPAPMAPLKGEMLALADWLIPNETEAFSLLRSLGLSREPNGKIVKGRDPAIDVARRL
                     ANVTGSNVVVTFGDKGCAYAGPLDREGIAFPAYEVDAIDTTGAGDTFVGALACVLDES
                     LGGSIDIATLIAHAQAAAAISTTSLGAQTSMPIRTEVEEFLAERGGGSGHGRQRG"
     gene            9486..11006
                     /locus_tag="PLU15_07395"
     CDS             9486..11006
                     /locus_tag="PLU15_07395"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO30785.1"
                     /translation="MGDKEVDPILSMEGICKSFPGVKALDGVDFSLWPKEVHALVGEN
                     GAGKSTLIKILAGVYKMDSGKILVEGRPVDISSPRQAQEHGISVVHQEFTLVPQMTVA
                     ENIMLSNMPSIPGLRSLVDKKTMVYESEKILNGLGVDIDVFAPVRLLTMAEQQIVEIA
                     KALSGKTRILVMDEPTAALSNVEVERLFDVIRTLVSKGIAVIYISHKLEEVFRISHRI
                     TVLRDGRVVSSSPISEVTRNEVVKQMVGRELTTMYDRCKADIGGPAMEVKGLLLDPAG
                     EPISFTVHEGEILGIAGLMGAGQIQLARALFGIGRTYGGEVLLRGRPASLSSPSKAVR
                     SGVGLVTENRKEEGLVQLLSVSKNITITVLDAISRRGVLSSWMEDRIVTEKIEELDIR
                     TPSPKQPVMNLSGGNQQKVVLAKWLAVDPDVMIMCEPTRGIDVGAKAEIYRLIGGMAK
                     NGKAVLLISSEIPEVVGMSDRVLVLHSGRVVGELMGDECTQEAVLRLATGGSVVGA"
     gene            10993..11946
                     /locus_tag="PLU15_07400"
     CDS             10993..11946
                     /locus_tag="PLU15_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOO30786.1"
                     /translation="MLAPRPVIDILRRYGMTFILLAMIAALSFMNPRFFSVSNFLTIM
                     RQVAINGALAAGMTMVILTGGIDLSVGGNVALAGCFAAGALQSTGNPWIAIVVALGVG
                     LMIGMVNGFFVSYAKLPPFIVTLATLALARGFTLVYTEGRPILVDSPIFEVIGGGFLA
                     GVPVPVLIVAVVYLIVGFVLNRTKFGRLVYAIGGNEQACRLSGVNVERTKMLVYVWCG
                     LLAGLSSVILSARLLSAQPTAGTGYELDAIAAVILGGTSLSGGEGGVGGTLVGAFIIG
                     VLANGLNLLNVSPFYQDVAKGAVILAAVLMDKAFHSTANAR"
     gene            11974..12393
                     /gene="rbsD"
                     /locus_tag="PLU15_07405"
     CDS             11974..12393
                     /gene="rbsD"
                     /locus_tag="PLU15_07405"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOO30787.1"
                     /translation="MKKSGIINPDLCRAIASLGHTDSIVICDAGLPIPQGVERIDLSL
                     VRGVPSFDQVFQEVVRELQVERAIVAEEALRENPHVIRVITEALGEGRVEFVPHLRLK
                     ELTEYAKTVVRTAEFRPYANVILYSGVEGLFEDGLPD"
     gene            12500..13399
                     /gene="rbsB"
                     /locus_tag="PLU15_07410"
     CDS             12500..13399
                     /gene="rbsB"
                     /locus_tag="PLU15_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HOO30788.1"
                     /translation="MRSNRILSVFLCVLMIGSMFASMASAAAPKIGLAISTLNNPFFV
                     DLRDGALEEAKKAGLGITVADAQNDPNRQLSQVENFIQQGVNIILVNPCDSAAIVSAI
                     KAANRAKIPVITVDRGADGGEVICHIASDNVQGGQMAGEYLAKLIGYKGKVVEIEGIP
                     GASAARDRGKGFNEVMKKYPNIKIVARQEAGFDRAKGMTVMENILQAQPDIDGVFCHN
                     DEMALGALRAIEAAGRLSAIKIVGFDATDDAIKAVQDGKLVATVAQKPRAMGSMAVTV
                     AKRVLDGAKVDAFIPVPLELVAK"
     assembly_gap    13580..13679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14773..15507
                     /gene="cysE"
                     /locus_tag="PLU15_07415"
     CDS             14773..15507
                     /gene="cysE"
                     /locus_tag="PLU15_07415"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOO30789.1"
                     /translation="MLKDIGSYIDAAMARDPAARNRLEVLLLYPGIHALIWHKLSHWL
                     YVNKAFGLARLTSQLVRFFTGIEIHPGATIGRGVFIDHGMGVVIGETAEVGDDVTIYQ
                     GVTLGGTGKETGKRHPTLEEGVVVSAGAIVLGSITVGSNSKIGAGAVVIRNVPPDSTV
                     VGVPGRVVARAGVRVREADLDHGDLPDPIGRALDLMALRVERLEAELETLRRISRSGQ
                     AVGYGGAPCEPLPESEEEEEAIEYCI"
     gene            15491..16417
                     /gene="cysK"
                     /locus_tag="PLU15_07420"
     CDS             15491..16417
                     /gene="cysK"
                     /locus_tag="PLU15_07420"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOO30790.1"
                     /translation="MSIASDVTGLIGHTPMVRLSRFGGGGRGEIIAKVESANPGGSVK
                     DRAALYMVDAAERAGVLAPGGTIIEPTSGNTGVGLAMIAAARGYRIVLVMPDSMSIER
                     QKLLAAYGAELVLTPGSHGMAGAVARARELAAEHSDWFMPLQFENPENCRAHEETTAR
                     EILADVEGRLDAFVAGIGTGGTVTGVGRALKREIPSVLVVGVEPQESAVISGGEPGLH
                     GIQGIGAGFVPSVLEMSILDEVISVSSDDAYRATRALAACEGLLVGISSGAVAVAALE
                     VARRLGEASRVVVLFPDTGERYLSVPGLWRVG"
     gene            16732..17664
                     /locus_tag="PLU15_07425"
     CDS             16732..17664
                     /locus_tag="PLU15_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO30791.1"
                     /translation="MNRRTNDDGSSALQKGFDAIARQNWFEAEEVFADFVDSEDASVE
                     AWLGWAIVQQSMGFRDEAASAVRQALAVLADDELEIQVLGELYSSIGLEQDALPFLLR
                     AADAASGDLDELERIAGLMLKIGYVRWGVDIAERVLAVDPERPEAWLIIGLSRFIDSR
                     WDEAVKAFEESLVRNPAHPMTHLSLGAARLEQNLLEQALEHLDFVIALPRNADEDLRY
                     ACSAMIRRARVLLLMGRRAEAVDTISEAAEIAGDSPTDLFDIGLFYLDDMGSANEALP
                     FLRKAAALDPYDSFKQATLAHAQLEAGEGVDSPR"
     gene            complement(17693..17983)
                     /locus_tag="PLU15_07430"
     CDS             complement(17693..17983)
                     /locus_tag="PLU15_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO30792.1"
                     /translation="MFDEAGFVVRATLATWTATDDGDDEPGKFKIRAIMPGNYSLRAT
                     AEGYIPAEVSGLVVHEGEDLELEEIWNEDCIRITGGEFDEEGRIVLLTHANR"
     gene            complement(17976..18548)
                     /locus_tag="PLU15_07435"
     CDS             complement(17976..18548)
                     /locus_tag="PLU15_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOO30793.1"
                     /translation="MQTGKWLPSTDTLAICLADAPIDTCEDVNIGIKKVFVKPFNSSD
                     QIIVAEFPDPVRVNLLDLRINEFVMTASISAGIYEDICLLLDAEGYVKLRGEDFAYPL
                     VAGDAEELDEDDDEQAIEIEYPFEVCSSRVTEILIDFDLAKVLQPNELGNGYVLDPDE
                     IEAVDKLDVGEIEGEVVATDHRQASCCRVV"
     assembly_gap    18932..19031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19032..20130
                     /locus_tag="PLU15_07440"
     CDS             <19032..20130
                     /locus_tag="PLU15_07440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOO30794.1"
                     /translation="RPDSSYSVPVDVECVALSRTRIYELDVFRAICTITVLLGHVSSY
                     PVGVLSTDSRFYGLYLGVNSFCRFAIQAFLFLSAAVLVHSYADRWQGPESALAMWRKR
                     LIDPGIPYVVWSVIYTLLAIRRGRHIDFPTFLRLLATGKAWDHLYFIVLIFQFYLLFP
                     LLLRALRSLSCRPVLWLGIGALIQAGFHMWDQRVFAIPNRASWAARFGFYLFAGMLAG
                     LDFDVLQDWTRRNRWAIAAVWAVSAVLNCSVSAAIRLGTPHRLSGYAELIVNVYAVAS
                     CLFFLAVSQWVTALNGWPMRAAMGISNASFGIYLSHPLGLAMWRRLTATGNPILFHLS
                     VWAGAVVVLALSWAATLWLKRFKFGWTLVGK"
     gene            complement(20198..21529)
                     /locus_tag="PLU15_07445"
     CDS             complement(20198..21529)
                     /locus_tag="PLU15_07445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOO30795.1"
                     /translation="MAKVIDWTIAIFGQYGLVGLFVVSFAESSFFPVPPDVMLIPLCL
                     ARPRASMLYATVATVSSVLGAVFAYLVGARGGRPLLLKFTSQDGLSKIQGLFKRFGGW
                     AVGIAAFTPIPFKVFTIASGIFQVRLAPFLIASTLGRGARFFLEAALVLKLGEQVRDI
                     IGTQFELITLMVGLVVAVAAIVWNRKSSVQAPIFSDKRGSGTKPYWRIRLPKSAASPG
                     LSGKRFWAEWGIYAIAGFVFLLIFFEELVELAQPDHAPFLRQIDMGIMAWIRAIRNPF
                     LSTTLTFTATLGSYPFVFACLALAVTYNLFRHRRARAATLAICVIGGLGLAVLTRWAY
                     SLPGPELALPVPDILSRSFAAQPIPVALPFYGMATHMLGSRTRSRARRRAIRAAAGVI
                     MALICFSGIYMGFQWPSEAAAGVIAGAMWLLICIRPLSLTGQGHKLGSKSG"
     gene            complement(21586..22458)
                     /locus_tag="PLU15_07450"
     CDS             complement(21586..22458)
                     /locus_tag="PLU15_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30796.1"
                     /translation="MEKGYDTQGRVDYGQIPEEQRGINSYASDNDTGSSCLKRAAKPR
                     SPLYTILVLAAIMVAMITYTRGDPRGRVVITPPWQPGEVSVLEIRDSHGQLIAAWELR
                     IDSDGKATLFTSDQHGSTYSEHAMVAADPDTLVPIRTELTYESDHGRIVYAALYGEDG
                     VSIEASVPGQNEESIVELPDTPYFDNEQFIMVIRALPLKRNFKATLKDVITRAAMKTT
                     ITLNVAKKEKLEVPAGSFETWKVDLMGTGRTVWIAVPHPHQIVRFEDRKARTTADLVQ
                     YTPGVELETGSEFE"
BASE COUNT         4903 a         5750 c         6934 g         5529 t
ORIGIN      
        1 gcgttggagc ccagcgagga ggcgggatgc agcattctct gcgtcgggca actacttttt
       61 aagcagtggt gtaacgagtg agcgaaagag gcttcggttt tgcgggagac cggcgcgagt
      121 ttgaagccag gcgcattcat gatcagcgtt gcagggattt cttgtggact gtgccgttgt
      181 ccttatgtga attacagtcg aacgcggtgg agccctcttg atcatcgccg taagcccatg
      241 tgatggcctg ttttgtcact gccttgtagt aatggtccat ggtctggatg aatgtccggc
      301 tcaagttggt gttgccttga tcccgcaaag gaagcggccc tagatgcttg atggcaacgg
      361 cgtcgataga gggaggcgca atggagatag caacatggtc taagtgctca acatcgccgt
      421 tccagaattc ctcgggggac gggcagagat cttcggccag cgcggctgca gcatctgtgc
      481 agcgcgttgt gttctcaggc tcggatgctt cctggcatgc tgcttgagag cgcttgcgcc
      541 tcaacgaaat tagcaggtct tcctttctca ttcccctcaa gaggaagagg agaaaagggt
      601 cgcggtcgaa ctcctgcgct aagacatagt ggacggctgc aatgtgcttg caggggttgg
      661 cccaatcagg gcaagtgcac gatgttgcca agtcttcctc tgagacgggg aagagggccg
      721 catcgcattg cgcgaaagct tcttcgatgt tctcaggcat ttcgccggcc aagaggctgg
      781 cggcaaacgc cgcgctttcg gccatggcgg ttgtcacgcg atcccagtcc gattcgttta
      841 ggggcttcat ccttatttga accttgtatg gccgagagcg ggaaccctgc accttggctt
      901 ccgccatgcc ggggctgaca tctacggaaa gaacattacc cgcccgcgca tatgacctgc
      961 ctctttgcag tcggtttgcc cagccgaaac tctccagggc aaaggtccac cttttcgccc
     1021 accagctgcc gccgaattcg tttttccgcg ccatgaacca atagcctccc atgcgccgct
     1081 cgggttatcc gccgagctat tgacgctagt cgtcgtcgat gatgctttcg tctctaaggg
     1141 tgaatatgtt gcgcaattca tcggtggaca tttccgtgat ccactcttcg cccgttccaa
     1201 ttatgctgtc cgccaatgct cttttctcat ctatcatctg gtcaattcgt tcttcaacgg
     1261 tgcctccgca gaggaatttg tagacattga cgtttttctt ttggcctatg cgaaaggccc
     1321 tgtccgttgc ctggtcctcc acggcggggt tccaccaacg gtcaaaatga aatacataac
     1381 ttgcctgcgt cagattgagc cccaggcctc cggctttgag ggagagaatg aagatgggcg
     1441 gcccgttggg atcgttttgg aaccgctcta tcattctgtc tctttccttt tgcggcacgc
     1501 caccgtgaag gaacaacact tcttgcctga gtgctttgga aaggtagtgc ttgaggagcg
     1561 ttcccatttc ggaaaactgc gtgaagataa gggctctgtc cccttcttcc actatctcat
     1621 cgagcatttc ctcaaggcgc gagagtttcc ctgaccgtcc ttccagcctg ctgttgtcat
     1681 gcaggaattg cgcaggatga ttgcagacct gtttcagttt gagaagagcc gcaagaatag
     1741 ctcctctccg ctccatgcct gaagcttctt cgatggtggc gatcatgtcg cggacaactg
     1801 cttcatacag ggttgcctgc tcctttgtga gattacaata gactttggtc tcatgctttt
     1861 ccggtaagtc ctgtattatt agcgggtcag ttttgactcg acgcaagatg aagggggcag
     1921 tcagcttgcg gagccgggcg gatgctgcgg catcgccata gcgctctatc ggcgttgcaa
     1981 agtgttgtct gaagcctgcg gctgagccaa ggtaaccggg gttgagaaaa tccatgatag
     2041 accacagttc aaagagacgg ttttctacgg gggtcccggt caaggccacc ctgaaatccg
     2101 cggtcagcct gcgggcgctc tgagcctgct ttgtgccgtg gttctttatg ttctgtgcct
     2161 catccagcac cagcgaattc cagtggagcg atgagagtgt atcctcgtcg cgagaaagca
     2221 gcgcgtaagt ggtgataacc acatcgcttt gcttggcttc ttgggcgaac tcgtctccta
     2281 tcagcctgtc tgatccgtga tggagcatca cattgaggga aggggcaaac ctggccagct
     2341 cccgcttcca gtttcccgct acagatgtag gacagacgag taatgacggg ccggggtcct
     2401 tctcgtaaag gagcagagcg atgatctgaa tcgtcttgcc aagccccata tcgtcagcga
     2461 gacaggcgcc tagcccccat tgcttcagaa aggctagcca ggagtagccc ctgatctggt
     2521 atgggcggag atttcctacg aatccttcgg gttgaggaag ctccgagatc ttctcgccgg
     2581 aattgagccg gtcgaggagc tctcccatcc agccatctgc ttcgatggct gtgacaggga
     2641 gtccgttttg agcttctatt tcctccccca tgcccaaaga tagagagagt atctcctgca
     2701 cactcatttg ctgtcgtcgc tttgggttgt tccagaacct aatggcggcc tcaacgtcat
     2761 caggccgtat tacaacccac tctcccctca cttttacgag cggcaccttc agcgaggcaa
     2821 gcctgtcaaa ttcctctgag ctcatctcct gatccccgat ggccactttc cagtcgtagt
     2881 ccaccagggt ggaggcggtg aggcggcttg acgctgcctg gccgcctact ttgagcgtga
     2941 tgcccacatt ggcagcgtta cctgaagttc gatcccacca tggtgggact agcgttccat
     3001 agccaccctc ttgcagaaga ggacagatat cgcgcagaaa ggagtatgcc tctgccggcg
     3061 agagttggca cgattcgggg catggatcct tcaggctttc ttcgacttgg gggatgaacg
     3121 agcccacacg ggccagatcg gcgagaagcc tctcctgcgg gttctcgaat cttcttccta
     3181 ggaaccgaag gacgcctccg ctttcctccc ataccttctc tgcgggtacg agcacagacg
     3241 ggtcgtctac cgcctgcaac aggtatctca aggtccatgg atcttctggg tcttcgggcg
     3301 tctccagctt gaagcatgtc cgaaacccgc cttgcggacc aggcgtttct gcttgagcgg
     3361 cccactcctt caccgctctg aacagggagc ggacgtcgga tttgtctccg ctgatgcgcg
     3421 agtcttctct ggccaggttt gcgatccacg agtgggtggg ggaaacgact cccaaaagct
     3481 ccatggtgag cctgttctct gcctcgtcgc cacgctggcc cagggcctgg gcacgtgaaa
     3541 tcatctcgct ccgttcgttt tcgaggatgg ctctaacagt ttcatccatt gctgtgcata
     3601 tgaagctttg aagcaaatcc tctttgcaaa ggatcgagtc cgttgcctga gcgagagtca
     3661 ttcctgcctc tccagagcgg gccttggtat gaggcaaaac cgccctcgag atgcccggca
     3721 tagcgtgggc cagtgcattg atgcgaagga tgtcggtttg ttcgtctaat acggggagcc
     3781 agactcccct gaaggaagag cactgtttgc gctctgatgc gcgggcgccc gctgcctggc
     3841 agatgcctgg aagaaagcgt tgccttgcca cgagctccag gacgagctta gcagccaacg
     3901 accaatatcg gacgtctgct cccagcatga ctggatgttg cccctgaagg ctgcatggca
     3961 ggatcctggc gctgtcaggc agcgcggaga agattttgac agcgtccgga gctgcaatgg
     4021 cgatcccgtt cacctggaat agttcgaagg tggcatgagc tttttggccg aagggatccg
     4081 gttcctccag agactctgtc tggctgccca ggtcgggtga tggctgggga taaccgcaca
     4141 ctgatggaag gagcagaagg gcgcggcgaa actctggcgg agcggatgtc agacgttcgc
     4201 tatcttgggc gccgctcgtt cctgttgcga ttgccgaaag aaccaatttg gcctcagcca
     4261 gctctgcgcg ggttgcttgg aaggggtgtt gtttgtaaac tccctggcgt ccgcgtgctt
     4321 tgtgcccgta gcgttgagga acttctgccc agataaagaa gactcctccg agagcgcgtg
     4381 cgtcggagat agctgttgaa gttgagactt tgcccggcgc ctgtgaagac gacgggagcc
     4441 atgttgcatg aatcatcaaa gcgcagtccc ccccgagaac aggttagcac agctcggaca
     4501 gggcagcaag ggagtctgct gattctgcga gatggccaaa ttgccatcca ctagcctgtg
     4561 ttttaagcaa gtctcgacac gtgtctgcgc gatgttgaag cttgtagctc tgcggaatag
     4621 gctttggctc ctcatcaaga ctgggaggct gcttaccgca gtgacgccct tttcgattca
     4681 accagcgcat ccaggagtct ttcagctttg cctgcatctt gaaccaatgg attattgatc
     4741 agggctagga gagctcggtt gtatgaatgg gtgacagccg cttctatggt gagctcctca
     4801 taagccttga cctgatgcat gagtccgcgt atctcgggct caatactgcc gatgtgaatg
     4861 gggtgggcgc ctgtggcgtc aattacggct ggaatttcca tgaccgatga cgccggaaag
     4921 ccgtcgaccg cgcctgcgtg agggatgtcg actatctcga ccttccccga gtcgttgtat
     4981 acagacgaca ttatgtctat ggagacggtg gaatagtggg cgccgcctcg cttggcaagg
     5041 gcaggaggtt ttgagactac agcgggatct ttgtagattc tcatcagctc tgcctctatt
     5101 tccattacct cttctgcccg ggtttttgga gaagattttt gctctgctat tgaggcatcc
     5161 gggcggtaga agtatttcag gtatggggat ggcaccatac tgagcgcctc tgccagcttt
     5221 ggagtcgccg gggtgccgga tatgtttttc tgcatggaag aggctgcaat ggccagcgcc
     5281 tgatcggtta cgtctctgcc cagccaatgc acctttcgta tccagctcaa gtggttcagc
     5341 ccaatgtaat cgagggagat ctcgttcgct gggactccca gagctcgagc tgcctcgagc
     5401 ttcatgttga tgggaacatt gcagaggccg atcgcgcgta ctctcccgtg gcgcgcaatg
     5461 gcttctgtaa tgattccgga ggggttggtg aagttgatca gccaagcgtc aggggccgct
     5521 tcgcgcatgg ttttgcagat ctcgaggatg ggaggaatgg tccttatagc cttggcaaat
     5581 cccgcgaacc cggtggtctc ttggccgatg agcccgtgtt ccatcgcgag tttgcagtcg
     5641 agatctcgcc agagttggcc tccgacgcgt atttgggtaa ctacaaagtc agcgccgact
     5701 attgcatcgc aggactgtgt atgcatctcg atgtttaagc tttccccgga tgcgtctacc
     5761 atccgcttga cgaaagagcc aacggtggtg agacgttctt tgtctatatc catgagactg
     5821 agggtttcca cggggaggtc ctggcggcga cggatgaggc cgtccacgag ctccggtgta
     5881 taagtgctgc cagcgcctac taatgtgagt ttcattgtaa gctctcactc ctgtcgaagg
     5941 ttgaatatga gtgctcgagc ctcttcatga attccctgct ggattctaca gccaaaccct
     6001 tgcctgccag gcgtgttgcg agtaagaccg cgccgaggac tggctcggcg gtagggacca
     6061 taatgcgtgc gttggggtat tcgggaagta ctgcctcgcg tacggcggtg agggtgatgg
     6121 ggtttgggtt ctgggctatg ctgcctgcca gaacgactgt tggatcatct tctggcgaaa
     6181 aaagctctct cagggctgca agggctgata ttcctagctc gcgcccgttg tcgataagta
     6241 tctccacggc tacgctgtct ccccgttctg ctgcttcgaa caccagcggg gcgatggatg
     6301 agaaggcaaa gccttcgcca tcggcgtagg tatggtggac tactgatagc aaatcggatg
     6361 agccaatatg gctacggatc aattgagaca gaagcgtggg gcgtccccgt ccgtcttcgc
     6421 ctctgatggc ggcggagtaa gctcgaattc cgatgacaga cccgcctgcg gcatcgccaa
     6481 aagcaggact cagtccgcca acctgttttc tttggccggt agcatttatc ccgatgcaat
     6541 tggttcctgt gccgcagatc acgacaactc cagtgccatc ctctgagcct agtgcgaggg
     6601 gtgcaagggc atcattgtcc acctcccagt gcggccacgg aggactgggc gttatcccgc
     6661 ctatcatgtc ctctatgatc tcgaagtcga ggggacgatc tgctccggaa agcgcgtaat
     6721 agacagcgag gcatcctcga ggagcggatg ttatctcggc gatttcggag atgacctgtc
     6781 tgatgttgga tgcggctaca tccttgcctg cgctttggta gttggcggga ccggatcttg
     6841 ccgcagcgat aatctctcct gcgagggtgg cggcgactgc gagggtcttg gtgcctccgc
     6901 cgtcaacacc tattacatat ggtgagtcct tggtagacat gccattgata cctgccttgt
     6961 tcttagtcgg tagattgcga agatagtctc acgatgatgt ccacgagagg attctccaca
     7021 agtgggcgtc ctcctgtccg tctttgtctg cccaagttta cttggtggcc atggctgtgg
     7081 agatatcagg gagcgggtag cctctttgtt atgtccgccg cattcgagat ctacaagaga
     7141 cgctggccga gagaaagagt gataatcgaa ttgtcccacc gttttgtatc cactgtggct
     7201 aaagcacagg cagaacccgc caaccgcgta ttcgtgaacg cgccctcggc ataacttgtc
     7261 ggccattcca aataattatt gacacaagga aggaattgca tactgtacat ggaatataaa
     7321 tgtggaaccg catacacatt gccatgacgc tacagaaaaa cgtcgaggca agggcggtta
     7381 gtagtgtgaa acctgttaca cggtgtctgt gttgatgcct agaatctgca atatagattt
     7441 ctcatcataa ggcgagagaa gcggttgcca gacaaccagt tatgagaaag tggtgggttg
     7501 caataagcac catatatgag gtggcgcaga aagcgggagt atcggtcgcc actgcctctc
     7561 gggcgctcaa tgacagcggt tacgtgagca gggagtcgag ggaccgtgtc ttggccgctg
     7621 caaaagcact ggactatcac ataaacgtca acgcgcgcag ccttaccagc gggaaaacgt
     7681 acaccatcgc tctggttctg cccgatgtca ccaacccatt ctttcctgcg gttgcaaggg
     7741 gtgttgaaga ctacgcttct gacaatggat attgcgtaat actgtgtaat acagacggta
     7801 acgcgcgcaa agagtctgac tatgtaagga tgttgcggtc cagaaaagtg gacggcgtga
     7861 tattcgcagt cagcgtggcg tcgaagtcga gggtaagcgc tttgacggac gatggaattg
     7921 cggtagtctt agtcgacaga gatttgggcg attcatgcga cattgtgaag accgacaata
     7981 tcgcaggagc attactggcc acaaatcacc ttattggcct cggccacacc agtatcgcct
     8041 ttatctccgg ccccatgtcg ctggcgacat ctcaggaacg ttttcgggga taccacgatg
     8101 cgcttgtttc aggaggagtt tccttcgatg aaagattggt ggtcgagggc gatttccgca
     8161 tgaagagcgg atatgaggcg atgcgcgacc ttctaagaaa gccgtgccag ttcactgccg
     8221 tctttgctgc caatgacctg atggcggtcg gcgcgatgac agcgcttgag gagagcggat
     8281 tccatgtgcc gaccgatgtg gctgttatgg gatacgacga tattgatatt gcgtcggtga
     8341 cgcgtccgcg tctgtcgact gttgcgcagc ccaagtatga aatgggttgg attgcggcgg
     8401 agacggtcat caggcggatg tgcaatccca aggcgtctcg catggaggag cttttgcagc
     8461 ctatgttggt cgtcagggat tccactgtgg aaaggagagg tgcttcggtg tgagcatagt
     8521 cgttgtggga agttgcaata tggactacat gatgcaggtc accagtcttc cgaaacgtgg
     8581 cgagacgatc tttgctgtca gccattctac tgcagcaggg ggaaagggag caaaccaggc
     8641 ggttgccgct gcgaggctgg gatctcgggt atacatggtt ggacgtgtgg gatctgacga
     8701 agcggggcgg gtccttgtgg agagcctgga aatggccggt gtagacgctt cgaaggtagg
     8761 caaagacgtg atggagtcga ccggttctgc ctatataacc gtggatcgag atggagctaa
     8821 taccatcgtc gtgaaccggg gcgccaattt cgccataaaa ccttcacatg tgacggccgc
     8881 tgaaagcctg attcaggagg cagatgttgt tgttgtgcag ctggagacgc cgattgaggt
     8941 agtcttccag gccttgaggt tagcacgaaa gcatggtgct gtgacggtgc tcaatcctgc
     9001 tccaatggcg ccgttgaaag gggagatgct ggcgcttgcc gactggctga ttccaaatga
     9061 gacggaggcg ttctccttgc tgcgttcgtt gggtctatcg cgggagccaa acggcaagat
     9121 agtgaaaggc agggatcctg ctatagatgt tgccagaagg ctggcgaatg tgacgggctc
     9181 taatgtggtt gtgacttttg gggacaaagg atgtgcatat gcaggcccgc tcgatcgcga
     9241 aggcatcgcc tttccagctt acgaggtaga cgcgatagac acgaccgggg cgggcgatac
     9301 atttgttgga gcgctcgcgt gcgtgttgga cgagagctta gggggttcga tagatattgc
     9361 cacgcttatt gcccatgcgc aggcagcggc agccatctcc actactagtc ttggagcgca
     9421 gacctcaatg cctatccgca ccgaggtgga agaattcttg gcggaaaggg gaggaggtag
     9481 tggacatggg cgacaaagag gttgatccta tcctcagcat ggaaggcata tgcaagagct
     9541 ttcccggagt aaaagccttg gacggcgtcg atttcagcct atggcccaaa gaggtgcatg
     9601 cgctggtagg cgagaacggg gccggaaagt ccactctcat caagatctta gctggcgtat
     9661 ataaaatgga ttccgggaag atcctggtcg aaggccggcc agttgacatc agttctccgc
     9721 gccaggctca agaacacggc attagcgtgg ttcaccaaga gttcacgctc gtgccgcaga
     9781 tgactgttgc tgagaacatc atgctatcga acatgccatc tattcctggg ctcaggagct
     9841 tggttgacaa gaaaaccatg gtctatgagt ctgagaagat cctcaacggc ctcggtgtgg
     9901 acattgacgt cttcgcgcct gtccggttac tgaccatggc cgagcagcag atagtggaga
     9961 ttgcaaaagc gttgtctgga aagacccgca tcctcgtgat ggatgaacct acagcagcct
    10021 tgtctaacgt cgaggtggag cggctgttcg atgtcatccg aaccctggtt tcgaagggca
    10081 tagctgttat ttatatctcc cacaaactcg aagaagtctt tcgaatatct cacaggatca
    10141 ctgtccttag agacgggcga gtcgtttcaa gttcgccgat ttcggaagtt acgcgaaacg
    10201 aagtcgtcaa acagatggta ggccgcgagc tgacaacgat gtacgataga tgcaaggcgg
    10261 acattggagg tcctgcaatg gaagttaagg gcttgctttt ggatccggca ggagagccta
    10321 tatccttcac agttcacgaa ggcgagatcc tgggaatcgc aggcttgatg ggcgccggtc
    10381 agattcagtt ggcgagagct cttttcggga tagggcggac gtacggcgga gaggttcttc
    10441 tccggggaag accggctagt ctctcatctc cgagcaaggc agtgagatcc ggtgtgggct
    10501 tggtgactga aaacagaaaa gaagaaggcc ttgttcagct gctttcagtt tcgaagaata
    10561 tcaccattac agttctggat gccatttccc gcaggggtgt gctctcttct tggatggagg
    10621 accgcattgt taccgagaaa atcgaggagt tggacataag aacgccgtct ccaaagcagc
    10681 ctgtcatgaa cttgtccggc ggaaaccagc agaaggtagt acttgcaaag tggcttgctg
    10741 ttgaccctga tgtgatgatc atgtgtgagc ccacgcgcgg catagatgtt ggggcgaagg
    10801 ctgagatcta caggctcata ggaggcatgg ccaagaacgg caaggcagtg ctcctgattt
    10861 cctctgagat acccgaggtt gttggaatga gcgatagagt tttggtgttg cattcgggca
    10921 gagtcgtagg cgaactgatg ggcgatgagt gcacacagga agcggtattg agattggcga
    10981 ctgggggtag tgttgttggc gcctaggcct gttatcgata ttctcagacg ttacgggatg
    11041 actttcatac ttttggccat gatcgctgcc ctttcattca tgaatccgcg gttcttctct
    11101 gtttcgaact ttttgacaat aatgaggcag gtggcgatca acggagcact ggcggcaggc
    11161 atgaccatgg tgatacttac gggagggata gacctttcgg tgggcggaaa tgtggcctta
    11221 gcggggtgtt ttgcagcagg cgctctgcaa tccacaggca acccttggat tgcgattgtc
    11281 gtggcgcttg gggtggggct catgatcggc atggtcaatg gcttctttgt ttcttatgcg
    11341 aagctcccgc catttattgt tactttggct acgttggcgc ttgccagagg ttttacgttg
    11401 gtgtacacgg agggcaggcc aatactggtc gattctccaa tcttcgaagt gataggcggc
    11461 ggtttcttgg ctggcgtgcc agtgccagtt cttatcgtgg cagtggtcta tttgatagtc
    11521 ggcttcgtgt tgaaccggac gaagtttgga cggcttgttt acgctatagg cggaaatgaa
    11581 caggcctgca ggctgtcggg agtaaacgtt gagagaacca agatgttagt ttacgtatgg
    11641 tgcggccttc tggcagggct gtcatcggtg attctatcag cgaggctgct ttcagcccag
    11701 cccactgcgg ggacgggtta cgagcttgat gccattgctg cagttatcct tgggggcacg
    11761 agcttgtccg ggggtgaagg cggcgtagga gggacgcttg tcggcgcttt cattattgga
    11821 gtgttggcga acggactcaa tctgctcaat gtgtctccgt tctaccagga tgtggccaaa
    11881 ggcgctgtga ttctggcggc tgtgctcatg gacaaggcct ttcacagcac tgccaatgcc
    11941 aggtgataga cgaaggatgg aggacgatag agaatgaaga aatccggcat aatcaacccc
    12001 gatttgtgta gggctatagc cagccttggg catacagact caattgtcat ctgcgatgct
    12061 ggactgccga ttccgcaagg cgttgagagg atagatttgt cattagtgcg cggtgtgccg
    12121 tcttttgatc aagtgttcca ggaggttgtt cgggagttgc aggttgaacg agcgatagtg
    12181 gcagaggaag cgctgaggga aaatccgcac gtgatcagag ttataacgga agctttgggc
    12241 gaaggtcgag tcgagttcgt gccccatctt aggctgaagg aactgacgga gtacgcgaag
    12301 acggttgtca ggactgctga gtttaggccc tatgccaatg tcatcttata ctcgggtgtt
    12361 gagggcctgt tcgaggacgg attgccggat tgactgggag gtgagcataa aggtcataca
    12421 cgggtcatac cgggcatact gtggatgtgt tgggagttta taggagacta tggcactatg
    12481 ataaggggag gcgctttgaa tgagatccaa taggatactt tcggtctttc tgtgtgtgct
    12541 gatgataggg agcatgtttg cgtcaatggc gagcgcagct gctccaaaga ttggtcttgc
    12601 gatttcaact ctcaacaacc cgttctttgt ggatctgaga gatggagctt tggaggaggc
    12661 caagaaggct gggctgggca ttactgtcgc tgatgctcag aatgacccca acagacagct
    12721 atcgcaggtg gaaaacttca ttcagcaagg ggtcaacatc attttggtca acccttgcga
    12781 ttctgcagcc atagtttcgg ccataaaggc ggcaaatcga gcgaagatac cggtgatcac
    12841 cgttgatcgc ggcgcagatg gcggtgaagt catctgccat attgcgtccg ataacgtgca
    12901 gggcggccag atggccggcg aatacttggc aaaacttatc ggctacaagg gaaaggtagt
    12961 agagatagag ggcatacctg gcgcttctgc tgcgcgcgac cgggggaaag gcttcaatga
    13021 ggtgatgaag aaatacccga acatcaagat tgtcgcgaga caagaagcgg gatttgacag
    13081 ggcgaagggc atgacagtca tggagaacat tctccaggcc cagccggaca tagatggggt
    13141 gttctgccac aacgacgaga tggcgttggg cgcgctcagg gccatagagg ctgctggcag
    13201 gctgtctgcc atcaagatag tcggatttga cgctacggac gatgccataa aagcagtgca
    13261 agacggcaag ttggttgcca ctgtagccca gaagcctcgc gcaatgggga gcatggcagt
    13321 gacagtggca aaacgcgtgc ttgatggggc taaggtggat gccttcatac ctgttccgct
    13381 ggagctcgtg gctaagtagt catcgagttg catagagaat aggttataga ctgtggagcg
    13441 gttgtggcca aggccacttg gctgcagccg cttcatgtcg cctacctaac gcctataggc
    13501 gctacccaag tcagttgttg gctggcgcgc aaggcattgg tttgatctgc atgtgctatg
    13561 ttagcgtcgc agcgtcaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    13681 catccggaga caatgctaag taggtctcga tacgtttctg tgcgatgtgt tacctgcggt
    13741 tcaaacctgg accgtttgat ctggacatga tgctgcttgt gattgctcag gtatgtccaa
    13801 aaaagcgttt gagtgttccc cggggcagct gaggcccttg cgccggatag caatcgagtt
    13861 caacagtcgc gcaaacagcg ccgcaaggct gggctgccct ttgagacccg gacaagaagt
    13921 gtgtggcggg ggagaaaggg ggtgtcaacg gacttggcca acttcaagtc acatcgcgat
    13981 cgcaagtgtt caagtcttag acacatccca ttgatgcgaa ccagcggata ccttgttatg
    14041 gatatgctca ctcgcaaaaa acggtccaac gcgtgtcaac aggctgttcc cgagctaagt
    14101 atacgtgtgc aggcaaaatc caagaccaag ctgtgccgat tacttgactt gtctgaagta
    14161 ccgagaaagc cgcctatcgg caagaatttt tccaaagcta cttgggcgtt gtcttgaggc
    14221 agggatggtg ggattagaat ggaaggctga acgttcagat gggcccgacg ggatcatgtg
    14281 ctaggccaat aatttggaat actgggcttt tggctatctt gacagcagac gtcggaagac
    14341 atatgctata tcccaaacag caaagatgca agcggctatg agagggaggt cgggggcgga
    14401 ctgcccagag agagggaacg tcaccgggtg caagcgttcc cgggcaagcg cttcttgagt
    14461 gcgcccttga gcctggcgct gaaactgtct gcaggcagcg ggtgggagta ggtggccacg
    14521 ggtgcgcccg ttatcgcgtg ttccccattt cgagcaaggc tttgcctgcc aaagtgggga
    14581 gcctgagctg cggttggccg agcgcgcatt gtcggatatc ggccatccgt gagcagagtg
    14641 ggaccacgga cagatgagcc gtctctgtaa agaggcggct tttgtgttat ataggccttt
    14701 tcctggcagg gtcgcctctg cagactcgtt gctgtttaac acattacggc acagccgagg
    14761 agttgcatga aaatgctgaa ggatattggt tcatacatcg atgctgctat ggcgcgtgat
    14821 ccggcggcca gaaacaggtt ggaagtgttg cttttgtacc cgggcattca cgctttgata
    14881 tggcataagt tgagccattg gctatacgtg aacaaggcat tcgggcttgc caggctcact
    14941 tcacagctgg ttcgcttctt tacggggatc gagatacacc cgggagcgac cataggccga
    15001 ggtgtcttta tcgaccacgg catgggggtg gttatcggag agactgctga ggtcggagat
    15061 gacgtcacca tctaccaggg cgttacatta ggaggtaccg ggaaggagac cggaaagcgc
    15121 caccccactt tggaagaagg ggtagttgtg tcggcaggcg ctatagtgct tggttcgata
    15181 acagtgggga gcaattcgaa gatcggtgct ggcgctgtcg ttattcgtaa tgttccacca
    15241 gattcgacgg tagtgggcgt tccaggccgt gttgttgcaa gagcaggcgt gagggttcgc
    15301 gaggccgatc tcgatcatgg cgatttgcca gaccccatag gacgcgctct cgacctcatg
    15361 gcgctcaggg tggaaaggtt ggaggcagag cttgagacat tgcgcagaat tagccgttct
    15421 gggcaggccg tgggatacgg aggggctcca tgtgagccgc tgcctgaatc ggaagaggag
    15481 gaagaggcaa ttgagtattg catctgatgt gaccggattg atcggccaca cgcctatggt
    15541 gcggcttagc cggtttggcg ggggcggtcg gggcgagatt attgctaagg tagaatcggc
    15601 caaccccgga gggagcgtga aagacagggc tgcactttac atggtagatg ctgcggagcg
    15661 tgcaggcgtt ctggcgccgg gaggcacaat aatcgagccg acgtctggta atacgggggt
    15721 ggggcttgcc atgattgctg cggccagagg ctacagaata gtccttgtta tgcccgattc
    15781 tatgagcatt gaaaggcaga agcttcttgc tgcatacggg gcggagcttg tgctgacccc
    15841 aggctcacat ggcatggcgg gcgctgtggc cagagctcgc gaattggcgg ccgaacactc
    15901 cgactggttt atgccgctgc aatttgaaaa tcccgaaaac tgccgggctc atgaggaaac
    15961 aacggcccga gagatacttg cagacgtcga aggtcggcta gacgcattcg ttgccggaat
    16021 tggaaccggc ggaactgtga ccggtgtggg gcgggcatta aaacgtgaaa tcccgtcagt
    16081 actcgtggtg ggagttgagc cgcaggagtc agctgttata tccggcggcg agcccggcct
    16141 ccacggcata cagggcattg gagcgggttt tgttcccagc gtgctggaga tgtcgatcct
    16201 cgatgaagta atctcggtta gctctgacga tgcctatcgc gcgactcggg cacttgcggc
    16261 ctgcgaaggg ttgttggtgg gaatatcctc tggagctgtg gccgtggccg cgcttgaggt
    16321 agcgcgcaga ctgggagagg cgagccgggt tgttgtccta ttccctgaca ctggcgagcg
    16381 gtacctgagt gttcccggat tatggcgagt tggatgagct agccggttct gaatgcgaga
    16441 cctttgggat ctgttgttta ggagaggttg agcaaacaag gcagtcaagc cgtgcttttc
    16501 gcaccgcttg gcccatagac caaggtggac aatgacgcga ttgccggagg aattcaatgc
    16561 ctcctaatcg ccacttgaag gagaatcatg ttctgcgggg aaatttagat aggcccgatt
    16621 tcgatgcccg gttgtgggga cgaacaggtt cggtgtccga aaagaactgc tgtgcccgct
    16681 cagtctggag atgggggtag gcagcggata gcggccgagg aggagacaac agtgaacagg
    16741 cgcacgaatg atgatggcag cagcgccctc cagaagggct ttgatgctat tgcccggcag
    16801 aactggtttg aagcagaaga ggtttttgca gacttcgtgg attccgaaga tgctagcgtt
    16861 gaagcatggt tgggctgggc cattgtccag cagagcatgg gatttaggga cgaagccgca
    16921 tctgcagtca ggcaggcgtt ggctgtcctt gccgatgacg agttggaaat acaggtgctg
    16981 ggagaattgt attccagcat cggcctggaa caagatgcgc tcccattttt gctcagggcc
    17041 gcggacgccg cctccggcga tctggatgag ctggagcgca ttgccggttt gatgctgaag
    17101 atcggctacg tgagatgggg agttgacata gccgagcgtg ttctggcggt tgaccctgaa
    17161 agaccggagg cttggcttat tatcggcctt agccggttca tcgattcccg ctgggatgag
    17221 gctgtgaagg ctttcgagga gagcctcgtg agaaatcccg ctcatcccat gacccacctc
    17281 tccctcggtg cggcaaggct ggaacagaac cttttggagc aggctttgga acatctcgat
    17341 tttgtcatag ccctgccgag gaatgccgac gaagacttga ggtacgcatg ttcagccatg
    17401 atcaggcgtg ctagagtcct gctgttgatg ggccgcagag cggaagctgt tgatactatt
    17461 tccgaggccg cggagattgc aggggacagt cctactgact tattcgacat aggccttttc
    17521 tacttggatg acatgggctc tgccaatgag gctctgccgt tccttcgcaa agctgcagcc
    17581 cttgatccat atgattcatt caagcaggct acccttgccc atgcgcagct ggaagcgggc
    17641 gagggtgttg acagccccag ataggcattt gccgttccaa tgtccgcaga cattatcggt
    17701 tcgcatgggt taggagaacg atccgtcctt cctcgtcgaa ctctccgcct gtgatgcgta
    17761 tgcaatcttc attccatatc tcttccagct cgagatcttc accctcgtgc acgacgagtc
    17821 cggatacttc ggcgggaata tagccttccg ctgtggcccg aaggctgtaa ttgcctggca
    17881 tgatggctct gatcttgaac ttgccgggct cgtcatcacc atcatccgtc gctgtccaag
    17941 tggctaaggt tgcgcgaacg acgaatccag cttcatcaaa caactctaca acaactggcc
    18001 tgtcgatgat cggtcgccac gacctcgcct tcgatttctc cgacgtcgag cttgtctacg
    18061 gcttcaattt catcaggatc tagcacgtaa ccgttgccga gttcgtttgg ctgaagaact
    18121 ttggcgaggt caaaatcgat aagaatctcg gttaccctgc tgctgcaaac ctcaaatgga
    18181 tactcgattt ctatggcttg ttcgtcatca tcctcatcaa gctcttctgc atcaccggcg
    18241 acaaggggat atgcaaaatc ctctcctcga agcttcacat agccttctgc atccagcaat
    18301 aggcaaatgt cctcgtaaat gccagccgaa atagatgcgg tcatcacgaa ctcgttgatc
    18361 ctcagatcga gcagattgac tctgacggga tcgggaaatt ctgctactat aatctggtcc
    18421 gaggaattga aaggcttcac gaagaccttt ttgattccga tgttcacgtc ttcgcaagtg
    18481 tctatgggag cgtctgcaag gcagatagcc agggtgtcgg tggatggaag ccatttgcca
    18541 gtctgcatcc tgggagcaat gccaggcaac cgcagacaat tgtgagtagc aacatagccg
    18601 caatcgagtg ttgtttcaat cgaaacgcct ccttgatcag catgatacct gcccattgtt
    18661 taatgcgaag ggaagaggat cgctgatttt gctgttaccg cgttggcagg tggcttaggg
    18721 catgatgcag aattcgtcct gagggatgga tgaacgtgag ggcttttgcg tatcaagttg
    18781 ccgctttcag gtttggcagg cataacttat cgatccgctg acatggttgg attgctccag
    18841 tccctctttg gcggtggccc gccggaacct ggtctttcgt gcggaaggac gggcgcattc
    18901 aggtgaaatt agcgcccttc gagccgctcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn ntcggccaga ttcatcttat tcagtgccag ttgatgtgga gtgtgttgca
    19081 ttgagcagaa ccaggattta tgaattagac gtatttcgcg caatctgcac cataactgtg
    19141 ctcttagggc atgtctcatc gtacccggtt ggcgttttga gcacggactc caggttttat
    19201 ggcctttatc taggcgtcaa ttccttctgt cggtttgcta tccaagcctt cctctttcta
    19261 agcgctgcgg tgcttgtcca cagctatgcc gatagatggc aggggccgga gagtgcgctt
    19321 gcgatgtggc gaaaaaggct tatcgatcct ggaattcctt acgtggtctg gtctgtgatc
    19381 tataccttgc tggctattcg gcgagggcgg cacatagact ttcctacctt tcttcgcctc
    19441 ttggccacgg gaaaggcctg ggaccatctg tattttatcg ttttgatatt ccagttctac
    19501 ttgctcttcc ccctgttgtt gagggctttg cgatctttga gctgcaggcc cgttttatgg
    19561 ctgggcatcg gggcgcttat tcaggccggg ttccacatgt gggatcaacg ggtatttgcc
    19621 atacccaaca gggcgagttg ggcggcgagg ttcggattct acttgttcgc cgggatgctt
    19681 gcgggtctcg atttcgatgt cctgcaggat tggacgaggc gaaataggtg ggcaattgcg
    19741 gcagtgtggg cggtgagcgc cgtgctcaac tgttcggtca gtgcagccat acgcctgggg
    19801 actccccatc gtctgtcggg atatgctgag ctgattgtga acgtgtatgc cgttgccagc
    19861 tgcctcttct tcctggcggt gtcgcaatgg gtgacggcgt taaacgggtg gcctatgcgc
    19921 gcggcgatgg gtatcagcaa tgcgtcgttc gggatttatc tctcgcatcc attgggattg
    19981 gccatgtgga ggcgtctgac tgccactggc aaccccatac tcttccacct gtcggtatgg
    20041 gccggggcag tggttgtttt ggcgctgtcg tgggctgcta ccctctggct caagcggttc
    20101 aagttcggct ggacgctggt gggaaagtag gggcactgcc cctagagggt ttggtatatg
    20161 accttcttgg ccagctcagg gtctgcaata agtctgccta gccagacttg gaaccgagtt
    20221 tgtggccttg ccctgtcaga gacagaggcc ggatgcatat gagtagccac atcgctcctg
    20281 cgatgacgcc ggcagctgcc tctgatggcc actgaaagcc catgtagatt ccggaaaagc
    20341 agatcaaggc catgatcacg ccggctgctg ccctaatggc ccgccgccgg gctcgtgatc
    20401 gggtgcggct gcccagcatg tgggttgcca taccgtagaa gggcagcgct acggggatgg
    20461 gctgggcggc gaagctgcgg gacaggatat cgggaaccgg aagcgcaagc tctggtcctg
    20521 gaaggctgta cgcccagcgg gtgagaactg ctaagcccag cccgccaatg acgcagatgg
    20581 caagggtggc ggctcgtgcg cgcctgtggc gaaaaaggtt gtaggttact gccagagcca
    20641 ggcaggcaaa gacgaaggga tacgatccga gagtggcggt gaatgtgagc gttgtgctga
    20701 gaaatgggtt tcgaatggcg cggatccatg ccataatgcc catatctatc tgccgcaaga
    20761 atggtgcgtg gtcaggctgt gcgagttcga cgagctcttc gaagaatatg agcaggaaga
    20821 cgaatcccgc aatggcatat atcccccact cagcccagaa cctcttgccc gacaaaccgg
    20881 ggctggcagc ggacttgggc agacgtatcc gccaataagg ttttgtcccg gaacccctct
    20941 tgtcgctgaa aatgggcgct tggacggaag acttcctgtt ccatactatg gctgcgacgg
    21001 ccacgaccag gccgaccatg agggtgatca attcgaactg tgttccgata atatccctta
    21061 cttgttcgcc aagcttcaag actagcgccg cttcgaggaa gaaccgggcg ccgcggccga
    21121 gggttgaagc tatgagaaag ggcgcgagtc gcacctgaaa gatgccggag gctatggtga
    21181 acaccttgaa gggtatcggg gtgaaggcgg ctatccccac tgcccagccg ccgaatcttt
    21241 taaatagtcc ttgaattttg ctaaggccgt cttggcttgt gaacttgagc agcagtggcc
    21301 gtccgccgcg cgctccaaca agatatgcaa atacggcgcc cagcaccgat gagactgtgg
    21361 caacagtcgc atacaacatc gaagctcgag ggcgcgccag gcatagcgga atgagcatta
    21421 cgtcaggggg gacaggaaag aagctcgatt ccgcgaatga gaccacaaaa agtcccacta
    21481 atccgtactg cccaaaaatc gctattgtcc agtcaatgac ctttgccata gttcaaccca
    21541 ccaatgtgtt ctttcctctc aggacaggcc tttgtcactt gtccactatt cgaattctga
    21601 gcctgtttcc agctccacgc ctggcgtgta ttgcacgagg tccgctgtgg tccgggcttt
    21661 ccgatcctcg aaccttacaa tctgatgggg atggggaacc gcaatccaga ccgtgcgtcc
    21721 agtgcccatc aggtcaacct tccacgtttc gaacgaaccg gcgggaacct ccaatttctc
    21781 cttcttcgcg acgttcagcg ttatcgtggt tttcatcgct gccctggtga tcacatcctt
    21841 caaggtggcc ttgaagttgc gcttcagggg aagggcgcgt atgaccatta tgaactgttc
    21901 attgtcgaag tacggggtgt cgggcagttc tacaatggac tcctcgttct ggcctggaac
    21961 cgatgcttct atagatacac catcttcgcc atacaaagca gcatagacaa tgcggccatg
    22021 gtcggattcg tacgtcaatt cggttctgat gggaacgagc gtatcaggat ctgcggccac
    22081 cattgcatgc tcgctatagg tggatccatg ctgatccgaa gtgaacaggg tagctttgcc
    22141 gtctgaatct attctcagtt cccacgcggc tatgagctgg ccatgggaat cgcgaatctc
    22201 aagcacactc acttccccgg gttgccaggg cggagtgatc actactcgtc cgcgcgggtc
    22261 gcccctggtg tatgtgatca ttgctaccat gattgctgca agcaccaaga tggtgtacag
    22321 gggcgatctg ggctttgcag ccctttttaa acagcttgat cccgtatcgt tgtcagatgc
    22381 atagctgttt attcctcttt gttcttctgg aatctgacca tagtcgacac gcccctgcgt
    22441 gtcatatcct ttctccacct agttccactc ctctcgctac tgaacagact gcatatgtgg
    22501 tcctgcacaa tcagattccc aaggctgaat acttcctgaa atccgatctt ccccttggcc
    22561 tttaggcgaa atacgattct caaggcccac tagttgggct gcccaaagcg ggcaaacgct
    22621 taccccaatg atacctttat gaacgcccac gctcaatacc tgatgcattt tgggttgccg
    22681 gggcgtatgc gacgacttat cagtagatgg gggaagtatt attctccagc cgccacaggc
    22741 atgctgaggc gcagcccgaa tctatgacat tgagtgatga cgacttgggc gtgatctaga
    22801 agcttattct acgacaaatg gcttggaacc tttccggcga gcctgtaaaa aggcggaatg
    22861 aagtgcaaga gcgaatgatt agttatgaga ggcttgtctg ccttgaaggg gtgccgaaga
    22921 tttgcgtcta ggtaactccc gataaggttc tgcgctaccg gttagcctgc catatgatgt
    22981 ctgctaggag tagacacgtc tttgcaccta tgtcgcagtg cggtaattgg ttcgcatttg
    23041 tgcatcgaca acgtacacat attggcctta accataaacg cccgtagcac tcagccgcgc
    23101 cgggatatga tccccggggc agggccaggg tagatggcgt aatcgaattc ccggttcgac
    23161 gctcgcgggc aaccatcttc tttacaacga cgaaagcgac gcgcatatac tcctgaccat
    23221 gagatccgtg tgagcgctcc gatggtttat gcatttaact tactcatatg aaagggcgac
    23281 ttgggtctgc cttcaaggaa tgtgtctatc tacggc
//
LOCUS       DAOSYL010000041        21972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1945_length_21972_cov_8.042296, whole genome shotgun sequence.
ACCESSION   DAOSYL010000041 DAOSYL010000000
VERSION     DAOSYL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_21972_cov_8.042296"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1178
                     /locus_tag="PLU15_07455"
     CDS             180..1178
                     /locus_tag="PLU15_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO30797.1"
                     /translation="MILVTGATGHIGNVLVRELIAKGESVRALVMPSDDERPIADLPV
                     DIAWGNVLVPITLERALEGIDTVYHLAGLVSIAPGQEELLESVNVGGTRNVIEACMKM
                     GVRRLVYCSSIHAIVEPPPGIPIDESCGFDPSKSRGDYDRSKARASLEVLNAVEQGLD
                     AVIVCPTGVIGPYDYLDSIMGQVIRDLASNSMKVLPAKGGYDFVDVRDVAAGLILAAE
                     KGRTGEAYILSGNYIDINSLGALVDELCGQKPVRRPRVPMWLVQACANCSDFFCNVTG
                     KKSRLTRYSIETLLSNANISSAKARQELGYSPRPLRESIADTLKWMAESDRLGTTA"
     gene            complement(1175..3118)
                     /locus_tag="PLU15_07460"
     CDS             complement(1175..3118)
                     /locus_tag="PLU15_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOO30798.1"
                     /translation="MHAAGRIRRGALCSVFGKKTIFVILALTAFMWTSSTLVDTPIEA
                     SVALACPIMEVGDIEPGMKGTGKTVFHGTEPEDFDVEVIAVLKGAGKLSDLVLIRASG
                     QAIEAAGGIAEGMSGSPVYIDGRLLGAISYVFAGSDHFMGMVTPISDMLRILSYPDST
                     GASLVKGTVGMTDRLWLDGALNAAVTPVSVSGLSGRSLERISQSLQQYGLTVRPSVSV
                     GSAKGRISVGSNSTVPSGVPDRGIPHIVPGSTVGVQLAKGDVDITAFGTVTYVDGDAF
                     LAFGHPLLGRGASALSACSASVQGIIKSESTPFKVLAPGATVGVITQDRLNGIGGRLG
                     QADAYVPVSIRVVDKETGRNRQISTFIAPDESLIADIFGAAALAAMDGVTDRMGLGTA
                     NVSLKIKMAGHDPYERANTFFSPSDVAGMSVGEPYDTLWLVANNAAEKVEIEGIEMYV
                     EVEPVRRTAMIESVRVMEPTVRPGEAVNVSVTLRQHRGGRVTKMMSVVVPEDAPGGVL
                     SMCVRGGNVSIFDEGDYVYDSMSMEGVLYMEVDDVMAKIASMPRGNELVLELEPYLFE
                     EEEHGSVVSPSLESHRDDLRDMGAKEKDQKTTVSTGAYEESSDKPTTARMETDWVIEG
                     IKWVTIEVETRDEGMEWCEGSEI"
     gene            complement(3137..4150)
                     /locus_tag="PLU15_07465"
     CDS             complement(3137..4150)
                     /locus_tag="PLU15_07465"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO30799.1"
                     /translation="MSSYGADIGIDFGSDNVRVYMPGKGVVIDEASVVVFHRETGAMI
                     EVGDRGRELLLADPGSCVPVRPLVFGSIRRHDAALLLMKRMIWRVLGGRGFTKPRVVL
                     TVPSCPSDVEIRAWMTMATEAGARSVMLLEAPMAAAMGADMNVFDARGQMMVDVGAEV
                     TDIGVIAVGEMIVSERVRTGGRAMDAAIQKHIRADKGLVLGDMAAEDVKRRVGVARNG
                     FEPSSAMVHGRDLETGLPRQEAISTEDVFEAISGKIDEIIEGIRCVLQKVPVEIGSDI
                     SSHRIVLAGGTSRLRGLSDAISEGVGLPVRVARDPEFCNIRGIGKFIADIDPRMTTAI
                     KSV"
     gene            complement(4257..4511)
                     /gene="spoIIID"
                     /locus_tag="PLU15_07470"
     CDS             complement(4257..4511)
                     /gene="spoIIID"
                     /locus_tag="PLU15_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOO30800.1"
                     /translation="MREYIRRRVLEITDHILETQATVRQTARLFNVSKSTVHKDVTER
                     LPSIDKQLAKKVKKILDQNKAERHLRGGEATRRKYKSGNK"
     gene            complement(4578..5267)
                     /locus_tag="PLU15_07475"
     CDS             complement(4578..5267)
                     /locus_tag="PLU15_07475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO30801.1"
                     /translation="MKWPFGSRSTSMMLVVGIAAFFLGFSLAILVSNVRRDTPAQIVG
                     HTDSGRFESEADEAIAIKPHEESGADSIEGVNAISAADSGSLSESEQGLVSMPEAMTA
                     PVDGSVVAGYGWRRDPVMDDWRFTDGIDISCLPGTQVRAALSGKVAGVDRDERGVLVV
                     RLEHAGGVETVYENLRIAWVKAGDEVGQGASLGEVGASSAASDGECIIRFIVLERGNA
                     IDPVQCIGKRF"
     gene            complement(5358..6341)
                     /gene="spoIID"
                     /locus_tag="PLU15_07480"
     CDS             complement(5358..6341)
                     /gene="spoIID"
                     /locus_tag="PLU15_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOO30802.1"
                     /translation="MSVSRFAWSLVSICVIVIIVLPGAIALFVRPRTAPGTTASLWVD
                     LWDCRAGTVRRMPLADYLVGVVAAEMPAAFDLEALKAQAVAARTYTLRKMWQTQETSA
                     STDVHKGAVICSDPSHCQAWSSREELLKKWGVAGYLANIRKVIAAVEETEGLVMTYNG
                     RLIDAVYHSCCGGMTEDAAAVWGRRVPYLVPVSCGCQQKALELGEMKTLEGAELVKAL
                     GLGSRSLPAMSSGVQVVARTATARASMVSVYGVPVSAADLRRALSLKSTRLAVVSSGG
                     KVTITTTGYGHGVGMCQWGAENLARAGLTFDAILKHYYSGIEIQRLDSSRK"
     gene            complement(6819..8096)
                     /gene="murA"
                     /locus_tag="PLU15_07485"
     CDS             complement(6819..8096)
                     /gene="murA"
                     /locus_tag="PLU15_07485"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO30803.1"
                     /translation="MDSFVIKGGESLHGRLRVGGAKNASLPILAASLLACGSSTVEDV
                     PELLDVETMCGVLTSVGARVSRSKNKVVVDATEISSFEPPYDLVRAMRASFLVMGPLL
                     ARMGCARIALPGGCAIGSRPIDLHLKGFAALGATIDMGYGQIEARADRLEGARVYLDF
                     PSVGATENIMMAAVLASGTTIIENAAQEPEIVDLANFLNSMGARVRGAGTTVLRIEGV
                     PELSPTSYMVVPDRIEAGTFMIAPAIAGGSLVIENVVSEHLKPITAKLRECGVTVEEN
                     DDGSMFVEMNDRPRGVDIKTMPYPGFPTDLQAQFMALMTICSGSSVITETVFENRFMH
                     VAELMRMGANIKIDGRSAVVEGQDKLLGAQVKCTDLRAGAALVLAGLAAEGDTEVADI
                     YHIDRGYENFEGKLASVGANIVRVASAEAEQAV"
     gene            complement(8195..9352)
                     /gene="wecB"
                     /locus_tag="PLU15_07490"
     CDS             complement(8195..9352)
                     /gene="wecB"
                     /locus_tag="PLU15_07490"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOO30804.1"
                     /translation="MGRIKVLSVFGTRPDTIKLAPVVAELSKYPDMISSVTVGTAQHR
                     DMMDQVLSVFGISPDYDLGIMQDDQTLFDIAERGLRGLEEVLVREKPDITLVHGDTST
                     AFVGALASFYCKTAIGHVEAGLRTYQKYDPFPEEMNRRLVGCLADLHFAPTADHLENL
                     LRENFPRENIYVTGNTVVDAVMRVAQSRTGYDDPKLASLDLEGKRLILVTAHRRENIG
                     EPLRNICAAIRDVLADYPDVVVVMPVHLNPRVRETVMEELSGAERCFLVDPVSYPDMV
                     GLMEACYMVMTDSGGLQEEAPALGKPVLVLRRTTERPEGLRTGTLRLVGVGREQIREE
                     AGILLEDAEQYRRMASAPNPYGDGNAAHRTVMGILHHYGLVDERPSDFMPI"
     gene            complement(9403..10434)
                     /locus_tag="PLU15_07495"
     CDS             complement(9403..10434)
                     /locus_tag="PLU15_07495"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOO30805.1"
                     /translation="MNRYLMSFLFTIASSFLLTPQIRAFALKRGIVDRPRGRHVHKAP
                     VPMLGGVAIYLSFAAACLIFTKVNKNAIGLLLGGGLIVAFGVWDDICEIKPRTKLCGQ
                     IAAAVVLVLFGVKIEFVTNPLGGMIYLGFMSVPLTIIWLVAFMNVVNFIDGLDGLAAG
                     VSAIGALAMAFAAFQTKQGQLVIMSMALAGSALGFLYYNFNPASIFMGDAGAMFLGFA
                     IAGISAMGALKTPATLALAVPVLVMGVAILDTAFAIFRRVRKGAPVSVGDKDHVHHRL
                     LAMGMTQREAVLTLYAVSGLLAAAACGIVVVNPRVGLGGAVVAFGALIIVGEKSGVMK
                     VGPRRKMDE"
     gene            complement(10558..11016)
                     /locus_tag="PLU15_07500"
     CDS             complement(10558..11016)
                     /locus_tag="PLU15_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOO30806.1"
                     /translation="MDGAGRERPSWDEYFMEIARVAAKRSTCLRRQVGAVAVRDKQIL
                     ATGYNGAPAGLAHCSEVGCVRAKLGIPSGERHELCRGLHAEQNLIIQAAVHGVAISGA
                     TVYCTFHPCVVCSKMLINAGIARIVFAGEYPDRLADEMLQEAGVELTRIP"
     gene            complement(11045..11674)
                     /gene="upp"
                     /locus_tag="PLU15_07505"
     CDS             complement(11045..11674)
                     /gene="upp"
                     /locus_tag="PLU15_07505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOO30807.1"
                     /translation="MGEVHVIDHPLIQHKLTLIRDKNTGPKDFRDLVGEVAMLMAYEV
                     TRDMQLESIEVETPIAHTSGKQLAGKKVAVVAILRAGLGMVDGVLKLIPAAKVGHIGL
                     YRDPDTLRPVEYYCKLPYDVPERELIMLDPMLATGGSACAAIQFAKDRGATNIKLVNL
                     IAAPEGIRLVQQAHPDVEIYVAAVDERLDSHGYIVPGLGDAGDRLFGTK"
     gene            complement(11698..12129)
                     /gene="rpiB"
                     /locus_tag="PLU15_07510"
     CDS             complement(11698..12129)
                     /gene="rpiB"
                     /locus_tag="PLU15_07510"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOO30808.1"
                     /translation="MKVAIGSDHAGYDAKEEVLKKLSDEGHDVVDFGTYSHESVDYPD
                     IAHAVAEAVASGECERGILLCGTGIGMSIAANKVRGVRAALCHDDFTAKMAREHNDAN
                     ILAVGSRTTAMHTIMRMVDIFLSTEFAGGRHADRVGKIEKA"
     gene            complement(12126..12584)
                     /locus_tag="PLU15_07515"
     CDS             complement(12126..12584)
                     /locus_tag="PLU15_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOO30809.1"
                     /translation="MPRKILLVCGGNTCRSPMAEAIMRKVIESEFPQMAGEIVVESAG
                     VAAVEGDTASENALTAAAKIGLDLSDHRARALTAKLISEADIILAMEGKHIASILAMD
                     PSAAGRVHTLADADIADPFGSSLEIYERTRDEIEAAVRRSLDVINRGDAG"
     gene            complement(12597..13763)
                     /locus_tag="PLU15_07520"
     CDS             complement(12597..13763)
                     /locus_tag="PLU15_07520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOO30810.1"
                     /translation="MSDAPEKRGKPTTVLIVVDPSGSAERLEAELGEAARVLRDGGTV
                     AFPTETVYGLGGNALDRRAVARIFAAKGRPQDNPLIVHIADMGALGSIASSVSPGARA
                     LADRFWPGPLTLILPKSDAIPDEVTAGLDSVAVRMPSHPVAAALIRAAGVPVAAPSAN
                     ISGRPSPTSAEHVWHDMAGRIDMLVDGGSADIGVESTVIDMTCSPPMILRPGGITLEE
                     IREVIPETVVLTGGEGLDGPAKSPGLRHRHYSPEAQMWLVIGEWDAQVDEIKSRAAAG
                     VRLGRRVGLLVSTETAAKLCTWIRDCGGEGPAGEIIVAEIGSRNDASQVASGLFDGMR
                     RLDAAEVDMMLAESYDARGMGLAIMNRLMRAAGGRIIRVQGEAGNDLISEKSIE"
     gene            complement(13794..14711)
                     /gene="prmC"
                     /locus_tag="PLU15_07525"
     CDS             complement(13794..14711)
                     /gene="prmC"
                     /locus_tag="PLU15_07525"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOO30811.1"
                     /translation="MGVTGRERQLLAILKLASEYLERQGVDSARLDAEVLLAHVLAMD
                     RIDLYVNYDRPMDDRELAEYRAVLRKRASRMPVAYITGFKEFMSLEFAVDERVLIPRP
                     ETEILVETVRDRITRASKERPRIADIGTGSGAIACSLALMLPSARVLATDVSSDALGV
                     AKANVKKHGLEDRVVCRQGDLLEGLGASMEREMVDAIVSNPPYIPSSELDALSPEISQ
                     YEPRGALDGGPDGLDFYRRLARGCVEFLADGGFLALEIGNGQAPDVIGLLTEAGFGDV
                     ESIDDYSGTARVVVASRPAGCRVLKNGAM"
     gene            complement(14722..15789)
                     /gene="prfA"
                     /locus_tag="PLU15_07530"
     CDS             complement(14722..15789)
                     /gene="prfA"
                     /locus_tag="PLU15_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOO30812.1"
                     /translation="MIGKLDEIEARYVELEQQIADPAVIADQITYRKLAKAHSDLEEI
                     VAKYRKLKDVMAEIVSVKEMLDGHLEPDMRELAEAELDELESRRGALESELRVLLLPS
                     DPRDEKNVIVEIRAGTGGDEAALFVGDLFRMYARYAERHGWELESMASSPTELGGFKE
                     IIFMISGDKVYSRLKYESGVHRVQRVPETEASGRIHTSAATVAVLPEAEEVDVEIDPN
                     DLRIDVFRSSGHGGQSVNTTDSAVRITHIPTGMVVTCQDEKSQHKNRDKALRVLRARL
                     LEQLTEEQEADIAETRKSQVGSGDRSERIRTYNFPQSRVTDHRIGLTTHRLAYVLDGD
                     LDEIIEALILHEQGEKLKRAE"
     gene            complement(15791..16678)
                     /locus_tag="PLU15_07535"
     CDS             complement(15791..16678)
                     /locus_tag="PLU15_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOO30813.1"
                     /translation="MNPRYQYGGQAVIEGVMMRGRDSAAIAVRRPDGDISVKMRDIDS
                     SKRPAILKAPFIRGVVTLIESLVVGMESLMYSANESSEEEEQLSGGEVVLSLVMAFGL
                     FILLFIVLPNVIVAFMQKHIPSVVLVNLLEGVLRIAIFLVYIVAISKMEDIQRVFAYH
                     GAEHKTIAAWEAGEELTVDNARKYSTLHPRCGTSFLLIVMVLSILVYSFLGEQTLGER
                     ILSRLILLPVLAGLSYEVLKLAGRPNPSVFVKWLSAPGLALQKLTTREPDDSMLEVAI
                     ASLNAVIEKDQASSEAAVI"
     gene            complement(16675..17277)
                     /locus_tag="PLU15_07540"
     CDS             complement(16675..17277)
                     /locus_tag="PLU15_07540"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOO30814.1"
                     /translation="MVGTGWIEVICGSMFSGKTEELIRRVKRARIARQNVVAVKPALD
                     IRFSSKDVVSHDGDKIGCLPVKDSDEAEAIIPDDVQVVAFDEAQFFDSGIVDLCVRLA
                     DRGVRVIVAGLDCDFRGEPFGSMPELLARAEYVTKLSAICVKCGNPATRTQRIIDGRP
                     ANYSDPQVVIGASEMYEARCRRCHQVPGKPTRNRTGGNQG"
     gene            complement(17402..17599)
                     /gene="rpmE"
                     /locus_tag="PLU15_07545"
     CDS             complement(17402..17599)
                     /gene="rpmE"
                     /locus_tag="PLU15_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOO30815.1"
                     /translation="MKDNIHPKFYITTVTCACGESFETGSTRENIRVDICSKCHPFST
                     GVQRVVDTGGRVEKFRKKHNL"
     gene            17805..18965
                     /locus_tag="PLU15_07550"
     CDS             17805..18965
                     /locus_tag="PLU15_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30816.1"
                     /translation="MSVLNRNALLARKFYRAKLTAQHADNMIKISFTRIRGCTIVLVR
                     DSTISMSSRYARMRQESVSESVRVRIGPETRPSAATSLVRDHVSISDSARAALARADD
                     SMSTDKSQALKGIPTDEASNDPKIRTIKRIIELLTGIKIETIELSEVVRSRQTQTPQS
                     MPEPEWSIEYDREYSYHDFQYMSFEAEGWITTEDGEQIKFSLSLSMRHEESAYSATSI
                     HIQNGRKIDPIIVNLDGEAAQLSDELFEFDLNADGKTEMIPGLHRGSGFLVLDRNGDG
                     IVNDGTELFGPSTGNGMNELAELDSDGNRWLDERDPAWTQLYIWTGGNPGDEPLVSLA
                     EAGIGAIYLGSVGAQFQMKNSANRPVGELARMGLYVRQNKTVGTMEQIDLIV"
     gene            19232..21733
                     /locus_tag="PLU15_07555"
     CDS             19232..21733
                     /locus_tag="PLU15_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOO30817.1"
                     /translation="MSHKGFTPLGRVLNLHIGDNQVSMETLDSRVDVSVIADDLVRVS
                     MTLLEPAHPRLPGSFSVIERQWPNPQVSIEDTPESVAISTPAVIVDISKDPLRIAFYN
                     PDGALICSDSAPRGMGWDELGAGCYKSFHPHTHFYGFGERTGGLDKRGTTMTLWNTDN
                     CPYTPGTDPLYISIPFFIAFSGESAYGLFMDTAARSTFNMGKPHPMLNSSKIPPQPTG
                     IPEVPEGEYWFGTSQKKLDYYFFAGPSIPDVVRGYAALTGYMKLPPLWALGYHQCRFS
                     YCPDSQVLEVAKRLREADIPCDAIYFDIDYMDGFRVFTFEPEDFPDPKELISQLSDMG
                     FKVVAIVDPGVKVDPDYSVYDKGRANQYFIKKADGKDYKGTVWPGTVSYPDFARADVR
                     SWWAENHSALFDAGVRGIWNDMNEPSNFDTESKTMDEDALHGSRGEEVAHAYVHNAYG
                     NLMSEATQEAFRRLLPKTRPFIISRAGCGGIQQSACVWTGDNSSWWEHLLMSIPMCLN
                     LSLSGVSFVGADVGGFLYDSDPELVTRWTQLGTFIPLFRNHSAIWTVPQEPYSLGEPY
                     ASICREFIKIRYRLIPYMYTLMKECADDGTPVMRPMVFEFPDDEKTHTMSDQFMLGPD
                     MMIAPIYMPGADCRPVYFPRGSWANLFNGNVISSLGESVLVGASLGQIPVFLREGAII
                     PWGMEMSYVGQHRQALEIIDIFPEEGPWERTFSLYVDDGETLGYQEGKFGSVEISCRT
                     SERETGRVLEISIDAQEDDYACAMAVRTLRIWNVAEEPVEVSVDGQRLPMLGNVCEVA
                     GGTGQTGYSYDEAGSRLCIGVHKISRNLEIVVAYR"
BASE COUNT         5216 a         6738 c         5141 g         4877 t
ORIGIN      
        1 ggaggccgcc ccattcatgg gcattggagt tttgcccggc tagtctgaga agaagcccga
       61 atgttgacag gagatctatt gagccatata ctgtttctga ctgaccattc agtcagtcag
      121 tggcagcatt tgcacacgag cgtcatctag cgcaatccat tccgaaggag caattccaca
      181 tgatattggt aacgggagca acaggccata taggcaatgt ccttgtgcgg gaactcatag
      241 ccaaaggcga aagcgtaaga gccttggtga tgccctccga tgacgagcgg ccaatagcag
      301 atcttcctgt tgacatagca tggggcaacg tgttggttcc cattactctg gagcgagcgc
      361 tcgaaggcat cgatactgtc taccatttag ccggcctagt cagcatagcc ccagggcagg
      421 aagaacttct tgaaagcgtc aatgtaggcg gcacccgaaa cgtcatcgag gcttgcatga
      481 aaatgggcgt tcggcgacta gtctactgca gctctatcca tgccattgtg gaaccgccac
      541 cgggaatacc catcgatgag tcctgcggat ttgatcctag caaatcccgg ggcgattatg
      601 atcgttccaa ggcccgagca tccctcgagg ttctcaatgc ggtagaacaa ggcctcgacg
      661 ccgtcattgt ctgtcccacg ggggtaatag ggccctatga ctacctcgat tccatcatgg
      721 ggcaggtcat acgagatctt gcctccaact ccatgaaggt tcttcccgcc aagggaggct
      781 acgattttgt ggacgtccga gatgtggccg ccggcctcat ccttgccgcc gaaaaaggaa
      841 gaaccggaga agcatacatc ctctccggaa attacatcga tatcaattca ttgggagccc
      901 tagtagacga gctgtgcggg caaaagccgg tgaggcgtcc ccgcgtgcct atgtggcttg
      961 ttcaggcatg tgccaactgc tcagactttt tctgtaacgt gaccgggaaa aaatcacgtc
     1021 ttacaagata ctccattgag acgcttctta gcaacgctaa tataagcagc gccaaggctc
     1081 gtcaggaact tggctattca ccgcggccat tgcgggaatc cattgcagat acgctgaaat
     1141 ggatggcaga gtcggatcgt ttggggacaa cagcctagat ctccgatcct tcacaccatt
     1201 ccattccctc gtctcgagtt tcaacctcta tcgtcaccca tttgatccct tcaatgaccc
     1261 aatccgtctc catgcgcgca gtcgtaggct tgtcggatga ttcctcataa gccccagtgg
     1321 agaccgtcgt cttttggtct ttctcctttg cacccatgtc ccgcaagtcg tctctatgtg
     1381 attcaaggct cggggacacg acagagccat gttcttcctc ttcgaacaag taaggttcga
     1441 gttcaagaac aagctcattc ccacgaggca tcgaggcaat tttcgccatc acatcgtcga
     1501 cttccatgta aaggactcct tccatggaca tcgaatcgta gacatagtct ccctcatcga
     1561 aaatgctcac gttgcctccg cgcacacaca tggaaagcac tccgccgggc gcatcctcag
     1621 ggaccacgac ggacatcatc ttcgtcacac gtccccctct atgctggcgc aaagtgacag
     1681 acacgttcac cgcctcgccc ggccgcacag taggctccat aactcgcacg ctctcgatca
     1741 ttgccgtccg acgcacgggc tcgacttcaa cgtacatttc tatgccttca atctccacct
     1801 tctctgcagc gttgtttgca accagccata gtgtgtcata gggctcacct acactcatcc
     1861 ccgccacatc agaagggctg aaaaacgtgt tcgcccgctc atatggatca tggccagcca
     1921 tctttatctt aagagaaaca tttgcggtcc caagtcccat tcgatctgta acgccgtcca
     1981 tggccgccaa ggccgcagcc ccaaatatgt cggcaataag cgactcgtcc ggagcaataa
     2041 acgtcgagat ttgtctgttg cgaccggtct ctttgtcaac cacccttatg gacacaggca
     2101 catacgcatc tgcctggccc aaccgcccgc caataccgtt cagacgatcc tgagtaataa
     2161 cccccactgt agctccggga gcaagaacct taaatggcgt agattcagat ttgattatgc
     2221 cttgaaccga cgcagagcac gcagacaatg cgctagctcc ccggcccagc agtggatggc
     2281 caaaggccag gaaagcgtcg ccgtctacat acgtaacagt cccaaacgca gtgatgtcga
     2341 catctccctt ggcaagctga actccaacgg tcgagccagg cacaatatgg ggtattcctc
     2401 tatccggaac ccctgaaggc accgtggaat tcgagccaac agatatgcgg cctttcgcac
     2461 tacccaccga aaccgaagga cgaaccgtca gtccatattg ctgcagtgat tgcgagattc
     2521 tctccaaaga ccttcccgag agcccactca cacttacggg ggttactgca gcattcagtg
     2581 ccccgtctag ccacaatcga tccgtcatac ccacagtccc cttgacgagg gaagcgccgg
     2641 ttgaatcggg atatgataag attctcagca tgtcggagat gggcgtgacc atacccatga
     2701 agtgatcgct tccggcaaaa acgtaggaaa tagcgcccaa caatctgccg tcgatataca
     2761 cgggacttcc gctcatcccc tcagctatcc cgccagcagc ctcaatcgcc tgacccgatg
     2821 cgcgtatgag tacgaggtcg ctcagcttcc cagcgccttt aagcaccgct attacctcta
     2881 catcaaaatc ttccggctcc gttccgtgga acacagtctt tcctgtgccc ttcatgccag
     2941 gctcgatatc gcccacctcc atgatcggac atgcaagagc aactgatgcc tcaattggag
     3001 tgtccaccaa tgtagagctt gtccacataa aggctgttaa ggccagaata acgaaaatag
     3061 tcttttttcc gaacaccgaa cacaacgccc ctctcctgat ccttcctgca gcgtgcaatt
     3121 ccaaccttcc tatctatcat acactcttta tggctgttgt catccgggga tctatatctg
     3181 caatgaactt gcctatcccc cttatattac agaattcggg atcacgagca actctaactg
     3241 gcaaccccac gccctcgctt atggcatcag aaagccctcg caggcgcgac gttcctccag
     3301 ccaatactat tctatggcta gaaatatccg atccgatctc cacaggaacc ttttgcagaa
     3361 cacatcggat tccctctatg atctcatcga ttttcccgct gattgcctca aacacatcct
     3421 cagtggaaat cgcctcctgg cgaggaaggc ctgtctccaa gtcccgacca tgtaccatgg
     3481 cagaactcgg ctcaaaaccg tttcgggcca caccgactct gcgtttgaca tcctctgcag
     3541 ccatgtctcc caacactagc cccttgtcgg cgcggatatg cttctgaatc gctgcatcca
     3601 ttgcgcgccc acccgtcctc acgcgttcgg agactatcat ttctccgacg gcaatcaccc
     3661 ctatgtcggt tacttctgca cccacgtcta ccatcatctg cccgcgagca tcgaatacgt
     3721 tcatatccgc gcccatcgcc gccgccattg gcgcttccag caacatcaca cttctcgccc
     3781 cagcttcagt cgccatggtc atccaagctc tgatctctac atctgatgga catgacggaa
     3841 ccgtcaacac caccctgggt ttcgtaaaac cgcgtcctcc cagcacccgc catatcatcc
     3901 gcttcattag caacaacgcc gcgtcgtgtc gacggatcga tccaaaaacc aaaggcctaa
     3961 cgggcacaca ggaccctggg tccgcaagca gcagctctcg accgcggtcg ccgacctcaa
     4021 tcatagcgcc tgtctccctg tgaaagacaa cgaccgaggc ctcgtctata acaactcctt
     4081 ttccaggcat gtacaccctc acgttatcag aaccgaagtc tattccgatg tccgccccgt
     4141 acgacgacat gttgcacccc caaaagcaga ttaagggaag gaccggcgcg ggcccgccta
     4201 tgccggtctt ctcgatggtc gcatctcagc ctttctcaga ttcccaccca cccctgtcat
     4261 ttgttgcccg acttgtattt tctccgggtc gcctcaccac ccctgagatg tctttccgcc
     4321 ttgttctgat ccagaatctt ctttactttt ttcgcaagct gcttgtctat ggaggggagc
     4381 ctctccgtaa catccttgtg gacggtgctc ttgctcacat taaacaggcg tgccgtctgt
     4441 cgcactgttg cctgggtctc caggatgtgg tcggttatct cgagaacacg ccggcggatg
     4501 tattccctca tctagcgtcc ggcctccccc ctgccgtttg ctagatctat attcgctcag
     4561 gggtggatta tgcctagcta gaagcgcttg ccgatgcact ggacagggtc gatcgcgttt
     4621 ccccgttcca aaacgatgaa tctgataatg cattcgccat cagaagccgc ggaactcgcg
     4681 ccaacctctc ccaaactagc gccctggcca acttcgtctc ccgccttgac ccaagcaatc
     4741 ctcaggttct cgtagacagt ttcaacaccc cctgcgtgtt ccaatcttac aaccaagact
     4801 ccacgttcat ccctgtccac gcccgcgact tttcctgata gagctgcacg aacctgagtc
     4861 ccagggaggc acgatatgtc gattccatca gtgaatctcc aatcatccat cacaggatcg
     4921 cgccgccaac catagcccgc aaccactgag ccatcgaccg gcgccgtcat ggcctcgggc
     4981 atactgacta agccctgctc cgattcgctg aggctgccgg agtcggccgc ggatatggca
     5041 ttcaccccct caatggagtc agccccagac tcctcgtgag gtttgattgc gattgcctca
     5101 tcggcctcgc tttcaaaacg gcccgagtct gtatggccta cgatttgagc cggcgtatcc
     5161 cggcgaacgt tgctcactag tatggccaag gagaatccga gaaagaaagc ggctatccca
     5221 accacgagca tcatcgacgt agatctgctt ccaaacggcc atttcataaa atcacctcgg
     5281 gttctaattc ttccccgagg cctcatccag tatgcttgct gatctccaga gcacgcgaac
     5341 tctcgataat gctcacatta cttgcggctt gagtcgagcc tctgtatttc gatgcccgag
     5401 tagtaatgct tgagtatggc atcaaaagtc aaacccgctc tggccaagtt ttcagccccc
     5461 cactgacaca tccctacgcc gtgcccatag cctgtggtcg tgatagtaac cttaccacca
     5521 ctagacacaa ctgccaggcg cgttgacttg agtgagagcg ctctccgcag atccgccgcg
     5581 gatacaggaa caccataaac cgacaccata ctagccctgg ccgtggcagt tctagccact
     5641 acctgaaccc ctgaagacat cgcaggaaga gacctgcttc caagtccgag cgccttcaca
     5701 agttctgcac cctcaagcgt cttcatctct ccgagctcca aagctttttg ctgacacccg
     5761 caactcaccg gaacaagata ggggacccgc cttccccaga cagcggctgc atcctctgtc
     5821 attcctccac agcatgagtg gtagactgcg tctatgagac gaccgttata tgtcatgaca
     5881 agtccctccg tctcctcaac cgcggcaatg acctttctta tgttagccag gtatcctgca
     5941 actccccatt ttttcagaag ttcctcccta gagctccatg cctggcaatg ggatgggtca
     6001 ctgcagataa ctgcgccttt atgaacatct gtcgaagctg aggtctcctg cgtctgccac
     6061 atcttgcgga gagtgtatgt ccgggcagcc acggcttgtg ccttcaatgc ttcgagatca
     6121 aaagctgctg gcatttctgc ggcaaccacg cccacgagat agtctgccag tggcattcgc
     6181 cgaactgtgc cggccctaca gtcccacaga tcaacccaga gagaagctgt agtgccaggc
     6241 gccgtcctag gacgcacaaa cagagctatg gcgccaggca gtacaattat gacaatgacg
     6301 catatagaaa ccaaagacca cgcaaatctg gacacagaca tgcatacctc ccctgccgcg
     6361 cacgaagctg tacagtctat gccctcattg aaaccgtaga acagccgagt tccgtccgat
     6421 acaatgctgt aaaaaggcct tagccctgtt gtagtggatg gggatggtat aaaaaggcca
     6481 ctggttctcc agtagaatgc accaactatc tactaggagg agaaccaatg gctacatatc
     6541 agattagtat atccgaagag caattgcgag gacactctct gcatgccagt tgcatcctac
     6601 aagcctcaac atgcttttac cattttcgac caaatggacc ggactcggaa ataacgcggt
     6661 caatacataa tagtgactgc taataccata cagaactctg ccggccaagg cctccagcac
     6721 ataaaaaaag acgcctgcat caagacgttc tcagatatcc ttctataggt ccaggtagtt
     6781 gtgggtacag ttcgcaagcc acggaaagcg tgacaatgct atactgcctg ttcggcttcg
     6841 gcagacgcaa cccgcacaat gttcgcgcca acgctcgcca gtttgccttc gaagttctca
     6901 tagccccggt caatatggta tatgtctgcc acctcagtat ccccttcagc tgcaagacct
     6961 gcaagaacca gcgctgcgcc ggccctgaga tcggtacact tcacctgtgc accaaggagc
     7021 ttgtcctgcc cttcaacaac agccgaacgc ccatctatct tgatgtttgc acccatccgc
     7081 atcagctctg caacgtgcat gaacctgttt tcaaagacgg tctccgttat cactgaagat
     7141 cccgagcata tagtcatcaa tgccatgaat tgggcttgaa gatcggtagg gaaccccggg
     7201 taaggcattg tcttaatatc cacgcctctt ggacgatcgt tcatctccac gaacatcgac
     7261 ccgtcatcgt tctcctcaac tgtgacaccg cattcgcgga gtttcgcagt gatcggtttc
     7321 aagtgttccg acacgacgtt ctctataacg agcgacccac cggcaatcgc aggagctatc
     7381 ataaaagtgc ccgcttctat tctgtcggga accaccatgt aagacgttgg agatagctct
     7441 ggcacgcctt cgatccgcag aacggtagtt ccggctcccc tcacacgtgc acccatcgag
     7501 ttcagaaagt ttgcaaggtc aacaatctca ggttcctgag ctgcgttttc tataatagtc
     7561 gtcccagacg ccaacactgc agccatcatg atattctcag tagcacccac actagggaag
     7621 tccaaataaa ccctggcccc ctccagtcga tccgccctgg cctctatctg cccatatccc
     7681 atgtctattg tggcgcctag cgccgcaaaa ccctttaggt gaagatcgat aggacgagat
     7741 cctatggcgc atccccccgg aagcgcaatc ctggcacagc ccatgcgtgc aagaagcggc
     7801 cccataacca agaaagacgc ccgcatcgcc ctcaccagat cgtagggagg ctcaaacgac
     7861 gaaatctcgg tagcgtcaac aacgaccttg tttttcgacc ttgatacacg agcgccgaca
     7921 gatgtgagca caccacacat agtctcaaca tcaagcaatt caggaacatc ttctaccgta
     7981 gaagaaccac aggcaagcaa ggaagctgct agtataggca aagaggcgtt cttcgctccc
     8041 cccacgcgca gcctgccgtg tagggactca ccacctttga taacgaaaga atccacttta
     8101 cctcccccaa ttccaaagca aacaggcatt ctgccccctt gcgcagttta acatcgggtc
     8161 tatccagagc acaaccggga atatcttccc ggtactatat gggcataaaa tcggaaggtc
     8221 gttcgtcaac gagcccataa tggtggagta ttcccataac tgttctgtga gcggcatttc
     8281 cgtcaccata agggttaggc gcgcttgcca ttctccgata ttgttctgca tcctcaagaa
     8341 gaatacctgc ttcttcccga atctgctctc tacccactcc aactagtcgc aaagttccgg
     8401 ttcgaagccc ctcaggcctc tcagttgtcc gtctgagcac cagcactggc ttgcccaaag
     8461 caggtgcttc ctcctggagg cctccggagt cggtcatcac catgtagcat gcttccatga
     8521 ggcccaccat gtcggggtag cttaccggat caacaagaaa acacctttca gccccgctaa
     8581 gctcctccat tacggtctcc cttaccctcg gattgagatg aacaggcatc acaaccacaa
     8641 catcgggata gtccgccaac acatcacgga tagccgcgca gatattccgc aaaggttcac
     8701 ctatgttctc acgccgatga gcagtgacca agattagcct cttgccctcg agatctaggg
     8761 acgcgagctt cggatcatcg taccccgtac gactctgggc aaccctcatt acagcatcga
     8821 ctacagtgtt ccccgtaacg tagatgtttt cgcgggggaa attctcccga agcaaattct
     8881 ccaggtgatc agcagtgggc gcaaagtgca gatcggcaag gcagcccaca agccttcggt
     8941 tcatttcctc cggaaaaggg tcatacttct gatatgtgcg caaaccagct tctacgtgac
     9001 caatagcagt cttgcaatag aatgaagcaa gcgctccaac gaacgcagtg gaagtatcgc
     9061 catgaacaag agtaatgtcc ggtttttcac ggactaatac ttcctccagg ccacggaggc
     9121 ctctctctgc aatgtcgaaa agcgtctggt catcctgcat tatgccaaga tcatagtcgg
     9181 gtgaaatgcc aaacactgaa agtacttgat ccatcatgtc acggtgctgc gcggtcccaa
     9241 ctgtcacaga gctgatcata tccggatact tcgaaagctc ggcaactacg ggcgcaagtt
     9301 ttatcgtgtc tggccttgtg ccaaaaacag acagaacctt aatccttccc atgaaacaac
     9361 ctccgacata ccatcaatga gcaacgcaaa cgtgcaagac ctctattcgt ccatcttccg
     9421 acgtggcccg accttcataa ccccggactt ctcacctacg atgattagag caccaaatgc
     9481 aacgaccgcc ccgcctaggc caactcgagg gttcacaacc actatcccac acgctgcagc
     9541 tgcaaggagc ccgctaactg cgtatagcgt gagaacagct tcacgctggg tcattcccat
     9601 ggccaacaac ctatggtgaa cgtgatcctt gtcgccgaca gaaactggcg cccctttgcg
     9661 aacacgcctg aagatggcaa aagccgtatc aagtatcgca acccccatca ccagcaccgg
     9721 gactgccaac gccagtgttg ccggggtctt aagcgcgccc atggcagata tgccggctat
     9781 ggcaaaaccg agaaacattg ccccagcatc ccccatgaat atcgaagcgg ggttgaagtt
     9841 gtagtatagg aaacccaatg cactccccgc taacgccata gacatgataa cgagctgacc
     9901 ctgcttagtc tggaatgcgg caaaagccat tgcaagggcg cctatagccg acactccagc
     9961 agctaaacca tcgagaccat caatgaagtt gaccacattc atgaacgcca caagccatat
    10021 gattgtaagc ggcaccgaca tgaacccgag atagatcatg ccgccaagcg ggttagtcac
    10081 gaattcgatt tttaccccaa aaagaaccaa cacgacagca gctgcaattt gcccacaaag
    10141 cttggttcgt ggcttgatct cgcaaatatc gtcccacacc ccaaaagcca ctatgagacc
    10201 gcccccaaga agcaatccaa tggcattttt gttgaccttt gtaaatataa ggcatgctgc
    10261 agcgaatgac agatagattg ccactccccc cagcattggc acaggcgcct tgtggacatg
    10321 tctgcccctt ggacgatcga ctatgcctcg tttcagggca aatgcccgta tctggggagt
    10381 gagtagaaat gatgatgcaa ttgtgaacag gaaagacatc aaatacctgt tcaagttgtc
    10441 gcactcccat cggttcggta taaaacaatc atgacgccga atccaggcta gatccgtgtc
    10501 gatcactgcg gatggatctt gccccagttt cctcgcagaa gtctgtacac acggacgtca
    10561 aggaatccga gtcagttcta cgccggcttc ctgcagcatc tcgtctgcta atcgatccgg
    10621 atattccccc gcaaatacga ttctcgcgat ccccgcattg atcaacattt tagaacatac
    10681 aacgcacgga tggaacgtac agtaaactgt agctcctgat attgccacac cgtgcacagc
    10741 tgcctggatt atcaggttct gttcggcatg aagcccacgg cagagctcgt gccgctcacc
    10801 agaagggata ccgagcttcg ccctcacaca cccgacttca ctgcaatggg caagtccggc
    10861 aggagctcca ttataccccg tcgccaggat ttgcttgtcc cttactgcta ctgcaccgac
    10921 ttgccgtcta agacacgtag atctttttgc agccaccctt gctatctcca tgaaatactc
    10981 gtcccatgaa ggcctctccc ggcctgctcc atccatagcc ataccttctc tgcgcagacg
    11041 cgcgctattt ggttccgaac agcctgtctc cggcgtcgcc caggccaggc actatgtagc
    11101 catgggaatc gagtcgttca tctacggccg caacatagat ctcgacatca ggatgggcct
    11161 gctggacaag gcgaatgccc tccggcgccg caatcaagtt taccagcttg atgttggttg
    11221 cccctctgtc cttggcaaac tgaattgccg cacaggcaga gcctcctgta gcgagcatcg
    11281 gatcgagcat tatcaattcg cgttcaggca cgtcatatgg aagcttgcag tagtattcta
    11341 ccggccttag cgtgtcagga tcacggtaga gcccgatgtg tcccaccttt gcagcaggaa
    11401 tcaatttcag gaccccgtct accatgccaa gaccagccct gagaatggcg acaacagcca
    11461 ctttcttccc ggccaattgc ttgcctgacg tgtgcgctat cggggtctca acctcgatag
    11521 actccagctg catgtcgcgg gttacctcgt atgccatgag catggcaacc tcgcccacca
    11581 gatccctgaa gtccttcggt ccggtgttct tgtctctaat gagagttaac ttgtgctgta
    11641 tcaatggatg atcaattacg tggacttctc ccaatcagta cacccccgat agccttacta
    11701 ggccttttca atcttgccta ccctgtcagc gtgccttccc cctgcaaatt ccgtagacag
    11761 aaatatgtct accattcgca ttatcgtgtg catggcagta gtgcgggagc caacagccag
    11821 tatgttcgca tcattgtgct ctctggccat cttggccgtg aagtcgtcat ggcagagtgc
    11881 agccctcact ccgcgcacct tgttcgccgc aatcgacatc cctatcccgg tgccgcacag
    11941 gagtatgccc ctctcacact cgccactggc gactgcctca gccactgcgt gggcaatatc
    12001 aggatagtcc acactttcat gggaatatgt tccgaagtcc acgacatcgt ggccttcgtc
    12061 tgaaagcttc ttcaaaacct cttcctttgc atcgtaaccg gcatggtccg atccaatagc
    12121 cactttcaac cagcatcacc tcgattgatc acgtccaagg atcgcctcac agccgcctct
    12181 atctcgtccc tggtccgttc atatatctca agtgaactcc caaaagggtc cgcaatatct
    12241 gcatcagcaa gagtatgcac ccggccagct gctgatggat ccatcgccag aatgcttgcg
    12301 atgtgcttac cctccatcgc aaggattatg tcggcctccg agatcagctt cgctgtgagc
    12361 gctctggcac gatggtctga caggtcaagg ccgatctttg cggccgccgt caacgcattt
    12421 tccgacgcgg tatcgccctc tacagcagca acgcccgccg attcgaccac gatctcgccc
    12481 gccatttgag gaaattctga ctcgataacc tttcgcatga tggcctcagc catcgggctg
    12541 cggcacgtgt tcccgccgca gacaagcaga atcttacggg gcactgcgtt caccgcctat
    12601 tcgatggatt tctccgaaat cagatcattt cctgcctcgc cctgcactct tatgatccgc
    12661 ccgccagccg ccctcataag cctgttcatt atcgcaagcc ccatgcctcg agcatcatac
    12721 gattcagcca acatcatatc gacctctgcc gcatccagcc ttctcattcc atcgaagagc
    12781 cccgatgcaa cttgagaggc atcattcctc gacccgatct cagctacgat gatctcaccc
    12841 gccggccctt ccccaccaca atcgcgaatc cacgtgcaaa gtttcgcagc cgtctccgtg
    12901 ctcacgagga gccctacacg ccgccctagg cgcaccccag cggctgctcg gctcttgatc
    12961 tcatccacct gcgcatccca ctcgcctata accagccaca tctgtgcctc cggcgagtag
    13021 tgcctatgcc tcaggcctgg agactttgca ggcccatcca agccttcacc gcccgtcaac
    13081 actacagttt cggggatgac ctcacgtatc tcctcaaggg tgatccctcc cggccgcagt
    13141 atcatcggag gcgaacaagt catatcgatg acagtcgatt ccacaccaat gtcagcagac
    13201 cccccatcga caagcatgtc tatccgtccc gccatatcat gccacacatg ctctgccgaa
    13261 gtcggactcg ggcgccccga tatgtttgcg gacggcgccg ccacaggaac ccctgcagcg
    13321 cggatcagag ccgctgccac aggatgagat ggcattctca ccgccacact gtccaatccc
    13381 gcagtgacct catctggaat cgcgtcagac ttggggagaa tcagcgtgag aggtccaggc
    13441 caaaacctat cagccagcgc tctggcgccc ggcgacacac tcgaggcgat gctcccaagc
    13501 gcccccatat ccgctatatg gacgatgaga ggattatcct gtggtctccc ttttgcggca
    13561 aaaatgcggg ccactgcccg cctgtccagc gcatttcctc ccagcccata gacggtctct
    13621 gtaggaaacg caaccgttcc gccgtctcta agaacccgag cagcctcccc gagctccgct
    13681 tccagacgct cggccgagcc tgacgggtcc accacaatca gcacagtagt aggcttccct
    13741 cgtttctcgg gagcatcaga catcagcatc tcctccaacc aaatcatctg cccctacatt
    13801 gcaccgtttt tgagcacccg acaccccgct ggccgggacg ccaccacaac cctagccgtc
    13861 ccggagtaat catcgatgct ttccacatcg ccaaatccgg cctcggtcag aagaccgatt
    13921 acatccggtg cctgtccgtt ccctatttct agagccagga acccgccatc ggcgagaaac
    13981 tccacacaac ctcgggcaag ccttctataa aaatcgagcc catccgggcc accgtccaac
    14041 gcaccacgcg gctcatactg ggatatttcc gggctcaaag catccaactc agacgaggga
    14101 atatatgggg gattggaaac tatggcatcc accatctccc gctccatgct cgcccccaaa
    14161 ccctccagca agtcgccttg cctgcagacg acgcgatcct cgagcccgtg ctttttcacg
    14221 ttcgccttcg ctacacccaa ggcatcagag gacacgtcag ttgccaacac tcgggcactg
    14281 ggcaacatta gagccagaga acaagcgatt gcgccgcttc ccgtgcctat gtcggcaatc
    14341 cgcggccgtt ctttcgaagc gcgggtaatg cgatcgcgca ccgtttccac caagatctca
    14401 gtttcagggc ggggtatcag cacgcgctca tcaacggcaa attccaagga catgaactcc
    14461 ttgaatccag tgatataggc tacaggcatg cgagaggcac gctttctgag aacagcccgg
    14521 tattcggcca attctctatc gtccattggg cggtcgtagt tcacgtaaag atctatcctg
    14581 tccattgcca ggacatgcgc caacaatacc tcggcatcca gtctcgcaga gtccacgcct
    14641 tgtctttcca gatactctga agccaatttc aatatggcca gcaattgcct ctcacggcca
    14701 gttacaccca tctggatact cctattccgc ccttttcaac ttctcgccct gctcatgaag
    14761 gatgagcgcc tcgattattt catctaaatc tccatcgaga acataggcaa gcctgtgggt
    14821 tgtcagccct atcctgtggt ccgtcaccct actctggggg aagttgtagg ttctgatccg
    14881 ttcgcttcta tctcccgacc ctacctgact tttgcgcgtt tcggctatgt cggcctcttg
    14941 ttcctctgta agctgctcaa gaagcctggc cctcaagact ctgagagcct tgtctctatt
    15001 cttatgctgc gacttttcgt cctggcatgt aacgaccatg cctgtgggaa tgtgcgtaat
    15061 cctcacggca gagtcggtag tattgacgct ctgcccgccg tgccccgatg atctgaacac
    15121 atcgatcctc agatcgttcg gatcgatctc cacgtcgact tcctcggcct ccggcagaac
    15181 cgctacagtg gccgcagaag tgtgtatgcg gccagacgct tcagtctcag gcacacgctg
    15241 cactctgtgg acgccacttt cgtatttgag ccgactgtag accttgtccc cggaaatcat
    15301 gaatatgatc tctttgaagc ctccaagttc ggtgggactg gaagccatgc tctccaactc
    15361 ccacccatgg cgctcagcat accttgcgta catacggaac aggtcgccaa caaagagcgc
    15421 tgcctcgtct ccccctgttc ccgcccgtat ctccacgatg acgttttttt cgtctctggg
    15481 atcagatggg agaagaagaa cacggagctc gctttcgagc gcccctcgcc tcgactcgag
    15541 ttcatccaac tccgcctcgg cgagctcgcg catatccggt tccaaatgcc cgtcgagcat
    15601 ttccttgaca gacacgatct ccgccataac gtccttgagt tttcggtatt tggccactat
    15661 ctcctcgaga tctgaatggg ccttagcgag tttacgatac gtgatctgat cagcaatgac
    15721 cgccggatca gcgatctgct gctccaattc cacgtatctc gcctctattt catcgagctt
    15781 tcctatcatc ttatatcacc gccgcctcac ttgaggcctg atctttttct atcacggcat
    15841 ttagcgaagc tatcgccact tccaacatgg agtcgtccgg ctcgcgagtg gtcaacttct
    15901 gcaacgccaa gccgggagcg gacaaccact taacgaacac agacgggttg ggtctgcctg
    15961 ccagcttaag aacttcgtat gatagtcccg caagcactgg caagagaatc agccggctca
    16021 gtattcgttc gcctagggtc tgctcgccaa gaaagctgta tacgaggatg gacaagacca
    16081 tgacgatgag cagaaaactc gtcccacacc tcggatggag cgtactgtac ttccgtgcgt
    16141 tatccacagt cagctcctcg cctgcctccc atgcagcaat agtcttatgc tctgcgccat
    16201 ggtatgcaaa aacacgctgt atgtcctcca ttttcgaaat ggccacaata tagaccagga
    16261 atatcgcaat cctgaggact ccttctaaca ggttgaccaa cacaacgctg ggtatgtgtt
    16321 tttgcataaa agcgacaatg acattgggaa gcactatgaa aagaaggatg aagagcccga
    16381 acgccattac taggctgagg acgacttctc cccctgacag ctgctcttcc tcttctgatg
    16441 actcattggc cgagtacatc agtgactcca ttcccacaac gagggattcg atgagcgtca
    16501 ctactccgcg tatgaacgga gctttgagta tagctgggcg cttggaacta tcaatatcgc
    16561 gcatctttac cgatatgtcg ccgtcagggc gacggacggc aatcgccgcg ctgtcacggc
    16621 ctcgcatcat cacgccctcg atgacagcct ggcctccata ctggtaacga ggattcatcc
    16681 ttggttacct cctgtccggt tccgagtagg tttccctggc acttggtgac acctgcggca
    16741 ccttgcctca tacatctcgc tggcccctat gaccacctgt ggatccgagt agttcgcggg
    16801 cctgccgtcg atgatcctct gagttctggt tgcagggtta ccgcatttga cacatattgc
    16861 tgaaagtttc gtcacatact ccgcgcgcgc cagaagctcc ggcatggagc cgaagggttc
    16921 gcctctgaaa tcgcagtcta gccccgctac gataacgcgc acacctctgt cggcgagtcg
    16981 cacacataga tcgacaatgc cagaatcgaa aaactgcgcc tcgtcgaaag ccacaacctg
    17041 cacatcatcg gggatgatag cttcagcctc gtcagaatcc ttaacaggca agcatccgat
    17101 cttgtcgcca tcgtgcgaca ccacatcttt cgatgaaaac cgaatatcca aagcgggttt
    17161 gacagccact acgttctgcc tagctatcct tgcacgcttc actctacgaa ttagctcttc
    17221 agtcttgccg ctgaacatgg agccgcatat cacttcaatc cagcctgtac ccaccatgct
    17281 tccttgcaaa gcataccctc ctcgcaggga gactcaaaaa accggagccc ggcagggctc
    17341 cgactgtaca tgcctcttgg gggtggcccc atatactgaa gccgccccca agcaaaactg
    17401 gctaaagatt atgcttcttg cggaacttct caacccgacc gccggtatcg acgaccctct
    17461 gcacgccggt ggaaaacgga tggcactttg agcaaatatc aaccctgatg ttctcgcggg
    17521 tagacccagt ctcaaagctc tctccgcaag cgcaggtcac agtcgtaata tagaacttag
    17581 gatggatatt gtccttcaac ctcttcacct ccgaggcata gacctcacag ttgtccttga
    17641 ctagctcagt atagcacaga gaaaagccat attcaaatag gccctgcact cgaagacgga
    17701 ccacatttga ctagttgcag tccggcctcg gaacagagaa catgcagccc aacaggctga
    17761 gagcagcaaa tctgacggtt actagcaata attgatgccc tcgcgtgtct gtcctgaacc
    17821 gaaacgcgct tttggcccgc aagttctaca gagctaagct cacggcgcaa catgccgata
    17881 acatgataaa gatatctttc acgcgcatta gggggtgtac catagtgttg gtaagagact
    17941 cgacgatatc gatgtcttcc cggtatgcgc gaatgcgaca agagagcgtg tcggagagcg
    18001 ttcgggtgcg gataggcccc gaaacccgcc catcggcagc gaccagtttg gttcgtgacc
    18061 atgtcagcat atccgatagt gcccgcgccg ctctggctcg agcagacgac tccatgtcaa
    18121 ccgacaagtc gcaggcgctg aaagggattc ccacggacga agcctccaac gatccgaaga
    18181 tccgcacaat taagcggatc atagaactgt taacaggtat caagattgag acaatcgagc
    18241 tttccgaagt agtaagaagc cgccaaactc agactcctca gtccatgccg gagcccgaat
    18301 ggagcattga gtatgacagg gagtattcat accacgattt ccagtacatg tccttcgaag
    18361 cagagggctg gataaccact gaggacggcg agcaaataaa gttctccctt agcctttcga
    18421 tgcgccacga agaatcagca tacagcgcaa cgtcgattca tatccaaaat ggcagaaaaa
    18481 tcgatcccat catcgtaaat ctcgacggcg aagccgccca actctccgat gagctgttcg
    18541 agttcgattt gaatgcggac ggcaagacag agatgatccc gggactccac cgaggcagcg
    18601 gtttccttgt tctcgacaga aacggcgatg gcatagtaaa cgacggaacc gagctattcg
    18661 gcccgtcgac cggaaacggc atgaatgaac tggccgagct cgactcggac ggcaacagat
    18721 ggctcgatga acgcgacccg gcctggaccc agttgtatat ctggactgga ggcaatccag
    18781 gcgatgaacc cctcgtatcc ttagccgaag caggaatcgg agccatatat cttggttccg
    18841 tgggcgccca attccagatg aaaaacagcg ccaaccgccc tgtaggagag ttggcccgca
    18901 tgggacttta tgtccgccaa aacaagaccg tcggcaccat ggagcagatc gacctcatag
    18961 tctaatcaaa ttccgaatgc gatctccgcc gcaaggaagg gcaatagagg ccccgttaaa
    19021 acgcacaact ttgcctgcta tgccctctcc ctgtttggcc gctgatacac cccttcacta
    19081 ccttgtagtc cgtcatcgct ccaatcaacc ccatacttgg acactggccc actcgcacac
    19141 ccgggagggt attggagatc ggtctagaac tacgcaaatg tcattagcca aaacgctcta
    19201 atgccacaga ttttgcatgg aggatcacaa aatgtcacac aaaggtttca ccccactcgg
    19261 ccgggttctc aacctgcaca ttggcgacaa tcaagtctct atggaaaccc tggattcgcg
    19321 cgtggatgta tctgtcatcg ccgacgacct cgtgcgagtg agcatgactt tgcttgaacc
    19381 agcccatccc cggcttccag gatccttttc cgtaatagag cgacaatggc ctaatcccca
    19441 agtgagcatc gaggacactc ccgaatcagt ggccatttcg acgcctgcag tcatcgtgga
    19501 catctcgaaa gaccctctta gaattgcatt ttacaacccg gacggagcac tgatttgcag
    19561 cgacagcgcc ccccgaggaa tgggctggga tgaacttggc gcagggtgct acaagtcctt
    19621 ccatcctcat actcacttct atgggtttgg ggaacggaca ggcggccttg acaagcgggg
    19681 cacgaccatg accctttgga acaccgacaa ctgcccgtac acgccgggca cagacccact
    19741 ttacatatct atcccattct tcatagcttt ctccggcgaa tccgcctacg gcctgttcat
    19801 ggacacagca gcaaggtcca catttaacat gggcaaaccc catcccatgt tgaacagctc
    19861 caagattccg cctcagccta caggtattcc cgaagtacca gagggcgaat actggttcgg
    19921 aacgtcacaa aagaagctgg actactactt cttcgcaggc cctagcatac cagacgtagt
    19981 ccgaggctac gcagcactga ccggctacat gaagttgccg cctctgtggg cattaggcta
    20041 ccaccagtgc aggttcagct actgccctga cagccaagtt ctggaggtcg ccaaacgatt
    20101 gagggaagca gatattcctt gcgatgccat atactttgac attgattaca tggacggttt
    20161 tcgagtattc acttttgagc cggaagactt tccggatccc aaagagctca tttctcagct
    20221 ttccgacatg ggcttcaaag tagtcgccat agtcgatccc ggagtcaagg ttgatcccga
    20281 ttattctgtt tacgataaag gccgagccaa tcaatatttc atcaaaaagg ccgacggaaa
    20341 agattacaag ggaacagtgt ggcccggaac agtctcatac cccgattttg cccgggccga
    20401 cgtgcgctcc tggtgggccg agaaccattc cgcccttttc gatgcaggag tgcggggcat
    20461 atggaatgac atgaatgaac cgtctaactt cgacacagaa agcaaaacga tggacgaaga
    20521 tgctctccac ggctctcgag gagaggaggt ggctcacgcc tacgtccata atgcttatgg
    20581 caacctcatg agcgaggcta cacaggaagc attccgcagg ctcctgccta aaactcgacc
    20641 attcattatc tcacgagccg gctgcggagg aatccagcaa agcgcgtgcg tctggactgg
    20701 agacaactcg agctggtggg agcatctcct catgtccatt ccgatgtgcc tcaatctaag
    20761 cctttcggga gtgtcatttg tcggcgccga cgtaggaggg ttcctgtatg attcagaccc
    20821 tgaactagtc acccgttgga ctcagcttgg cacattcatt ccgctcttcc gaaaccattc
    20881 agccatctgg actgtcccgc aggaacctta ttctctggga gaaccgtatg cgtcaatctg
    20941 ccgcgaattc attaagatcc gctataggct cattccatat atgtacacac tgatgaaaga
    21001 gtgtgccgat gacggaaccc ctgtaatgag gccaatggtg ttcgagttcc ctgacgacga
    21061 aaagacgcac acaatgagcg accagttcat gctcggtccc gacatgatga tagcgcctat
    21121 ctacatgcca ggcgctgatt gcagacccgt ctacttccca aggggcagtt gggccaacct
    21181 gttcaatggc aacgtgatat cgagccttgg ggaatccgtc ctcgtggggg cctccctcgg
    21241 ccagatcccg gtcttcctcc gcgaaggcgc cataatcccg tgggggatgg aaatgagtta
    21301 cgtcggccag caccgacagg cgctggagat catcgatatc ttcccagagg aaggtccttg
    21361 ggagcggacg ttttcgctat atgtggatga cggagagacc ctcggctatc aagagggcaa
    21421 attcggttca gtggagatat cctgccgtac atcagaaagg gaaaccggcc gagtcctaga
    21481 aatctccatc gacgcccaag aagatgacta cgcatgcgcg atggccgtaa ggaccctgcg
    21541 catatggaat gtggctgaag aaccagttga ggtgagcgta gacggtcaac gcctacctat
    21601 gctcggcaat gtgtgcgagg ttgcaggagg caccggacag acagggtatt cctacgacga
    21661 ggcaggaagt aggctttgca taggcgtcca caagatctct aggaatctgg agatagtcgt
    21721 ggcatatcgc taagtagacc gcaacagatc tctgcgcgat cctcgagttg tgctgtcgct
    21781 gtctgggact tagccggggg ctgaacttgt aagtttggcc ttgggtaaaa gcccagggaa
    21841 gcgccgtgca gcgtggagcc ggccaaggtt tgccgcgagg gcgagcaggt tttgcagacc
    21901 agtctaaatt ctagacattg aaggcaggcc caagttgccc tttcatatga gtgcgttgaa
    21961 tgcatagact gc
//
LOCUS       DAOSYL010000042        21653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_1982_length_21653_cov_7.377026, whole genome shotgun sequence.
ACCESSION   DAOSYL010000042 DAOSYL010000000
VERSION     DAOSYL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21653
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_21653_cov_7.377026"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLU15_07560"
     CDS             complement(<1..563)
                     /locus_tag="PLU15_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOO30818.1"
                     /translation="MADGQSGGRTLLRQYLKSGDLLMAAMVLLILLMMIVPIPPIVLD
                     VLLALNLAFSLGVLLVTMYMTEPLQFSVFPSLLLLSTLLRLSLNVSATKLILLRGYAG
                     SVIDAFGNFVVGGDYIVGLIIFVILVIIQFVVITNGAGRVAEVAARFTLDAMPGKQMS
                     IDADLNAGMITEEEARMRRRKIQQEAD"
     gene            complement(588..1652)
                     /gene="flhB"
                     /locus_tag="PLU15_07565"
     CDS             complement(588..1652)
                     /gene="flhB"
                     /locus_tag="PLU15_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOO30819.1"
                     /translation="MAKPSHEDRTEQPTPRRREEARKEGQVARSADLNAALGLIIGLM
                     ILESSFSFLGQTVANFMITRIGGLGSADCSQVGAMSLLMEGMITFLIAAAPVALGAMF
                     VGLVVNLAQVGFMFSPKLLVPKFSRIDPISGLGRIFSKEALVGLLKSAVKAVAIGYSG
                     YLALRTMIPQITMASYMAIDEGLATAADAGIYVVKQMSVLFVIIGVADYAYQRMELER
                     NLRMTKQELKEEIRQQEGDPMVKSRIRQRQRQIAMRRMMEDVKTADVVVVNPTEYAVA
                     LKYDQERMNAPVVVAKGQRLIAQRIREVAEEAGVPVVPNPPVARAIFSAVEIGQEIPP
                     ELYRAVAEILAFVYRLSKAR"
     gene            complement(1739..2521)
                     /gene="fliR"
                     /locus_tag="PLU15_07570"
     CDS             complement(1739..2521)
                     /gene="fliR"
                     /locus_tag="PLU15_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOO30820.1"
                     /translation="MAEGTLGFTEREFLLFFLVLVRMTGLFILAPLFGHASVPARAKA
                     GLAFACALVVYPMMAQTPFEMPTDVYELTSDVARELFVGATLGFVSLLVLAAAQYGGE
                     MIDTQIGFSLANIVDPSFGQQVSLLGQFHYLVAMMVYMAVDGHHYLIGALMRSYSVIP
                     IGGAGMSSELFSLVLARFSDLFMIAIRMSLPPVACLLITEIALGVLAKSVPQMNVLMV
                     GFPAKIVVGLAVVGLAIPGFVGCAATAFASLRKAIVVLMRAF"
     gene            complement(2543..2812)
                     /gene="fliQ"
                     /locus_tag="PLU15_07575"
     CDS             complement(2543..2812)
                     /gene="fliQ"
                     /locus_tag="PLU15_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOO30821.1"
                     /translation="MNEAMVAALASEAVSVAFKVSLPVLLTALVVGVLVSVLQAATQI
                     QELTLTFVPKLLAIAAVLLLTGSWMTSTLTGFAREVFAMLPLLAK"
     gene            complement(2840..3574)
                     /gene="fliP"
                     /locus_tag="PLU15_07580"
     CDS             complement(2840..3574)
                     /gene="fliP"
                     /locus_tag="PLU15_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722487.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOO30822.1"
                     /translation="MAGIGIALACSAVAYCQPMPIPNIRIGVDAAESPTEVAGALRII
                     ALLTVLSIAPAILLMTTSFTRIVIVLSFVRNAMGTQQVPPNQVLIGLALFVTLFVMMP
                     VYSQINEEALKPYMAGIISQEEAMARGVAPLRSFMLKQTREKDLALFVDLAGLPKPGG
                     PEDIPTYVIMPAFMVSELKTAFQMGFVIFIPFLVIDMLVASVLMSMGMMMLPPVLVSL
                     PFKVLMFVLVDGWGLVVKSLVESVAR"
     gene            complement(3649..4050)
                     /locus_tag="PLU15_07585"
     CDS             complement(3649..4050)
                     /locus_tag="PLU15_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOO30823.1"
                     /translation="MEESMAMLLLRAALALAAVAGLIYLVAWLLKKARASGMIKVSGP
                     ERRIRLIETARLGADSYLHLAEVDGKIALIGVGRGGISIVQWPRGSGQEGTGDMPDGD
                     GGLSAALYGQTDEESTDSQDGGGSEGGVDAV"
     gene            complement(4053..4301)
                     /gene="fliN"
                     /locus_tag="PLU15_07590"
                     /pseudo
     CDS             complement(4053..4301)
                     /gene="fliN"
                     /locus_tag="PLU15_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009965.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            complement(4531..5514)
                     /gene="fliM"
                     /locus_tag="PLU15_07595"
     CDS             complement(4531..5514)
                     /gene="fliM"
                     /locus_tag="PLU15_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOO30824.1"
                     /translation="MAEVLTQEEINALLAQFSKSEEEDQEHRGALSDGVSYTVYDFKR
                     PNRFSREQLRTIHMLHETFARYLSTTLSAHLRTPVKVTLIDVQQRIYDEYLSSLKSPA
                     VMYVFTPEPLDGQAIIEFNPYIVFIMIDRLMGGPGRVRGRVRELTEIEEKLVRAMVDR
                     AMDDLKDAWDPMVRFDPHIDAYESNPQFAQIVSPNDTTCVISFEIRVGEANGSMSICM
                     PHSTLQPVLQKLSAQQWYQKGTKISSEKSANAISAGLEGVKVPIAACLGRASITVSEM
                     LELQTGDVIKLDEHAFDPLTVVIGNSPKFHAIPGLVGKNYAIQIVGTVEST"
     gene            complement(5533..6021)
                     /locus_tag="PLU15_07600"
     CDS             complement(5533..6021)
                     /locus_tag="PLU15_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOO30825.1"
                     /translation="MKKAENGNKRKFKSKRKTTFAVAALLVGAILGYCLVARRAPGTE
                     SGASEMAQTEQTNADRDEIGPEYTFDTFIVNIADTNGHRFLKCTLTVEFDDKAGVTEA
                     AGKVPLLRDAIIDVLSSKTLEELEPGEPRDALRAELVEAVARLIAKADVKRVFFSEFV
                     VQ"
     gene            complement(6026..6775)
                     /locus_tag="PLU15_07605"
     CDS             complement(6026..6775)
                     /locus_tag="PLU15_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOO30826.1"
                     /translation="MRRRKRNDESGGSQPSWMTTYSDLMSLLLTFFIMLFAFSSIDMQ
                     RFREALISLQGALGVLSGGPALLEPGEMPIPPDDPSQIGDDTMSQARTDLVIIKGQLE
                     EYLEEKGLRGKVHVEMNKRGLLIRFTDTVLFDLGMATLRPDSRMLLRDLAKFIGPIQN
                     EVVVEGHTDNVPLRRGAQYETNWELSTARATTVVRYFIEELGLSPKRFSAAGYGEYRP
                     VLPNTSYANRELNRRVDILIIASDVTDIPGM"
     gene            complement(6772..7617)
                     /locus_tag="PLU15_07610"
     CDS             complement(6772..7617)
                     /locus_tag="PLU15_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOO30827.1"
                     /translation="MDSATVIGFILGVACILIALMQSDAGLRPFWDIQSVLITVGGAL
                     GAVIISFSAEQLKDIPKMIKIAFRSEEEDPLVIIDGMVDLAEMARREGLLALEDSLNR
                     TDDPFLQRGIQLIVDGTDPELVRDILETDLAYIEDRHRNGKAIFDAMTAYAPAFGMIG
                     TLIGLVLMLRTLDKPSTIGPAMAVALITTLYGAVIGNLVTGPIATKLGVKSAEEIRLR
                     EMMLEGILSIQSGENPRIVEEKLMGFLAPKDRLRAQERAGMRRGGARSRREMAEDTGG
                     SGTTL"
     gene            complement(7622..7846)
                     /locus_tag="PLU15_07615"
     CDS             complement(7622..7846)
                     /locus_tag="PLU15_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOO30828.1"
                     /translation="MIKVTKLNGSPIHINAELIETIAATPDTVITLTNGTKLIVEESV
                     EKVVDMIIRYHRLINRRAVIVRDSAEKQTR"
     gene            complement(7979..9241)
                     /locus_tag="PLU15_07620"
     CDS             complement(7979..9241)
                     /locus_tag="PLU15_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOO30829.1"
                     /translation="MMRSMFAGVSGTRAHQVRMDVIGNNIANVNTVGYKYGRATFQDM
                     LSQTMRGSMAPTGSRGGVDPMQVGMGVSTRSIDIVFTPGNLEYTGRLTDMAIQGSGLF
                     VVRDSSGGLMFTRDGAFKIDAEGNLTHPSTGFMVQGWMADAKGTIDRSGEIQGLRIPL
                     GTSMSAKATDRITFVGNLNAEGDVGTLHSTSVPIYDSLGIQHTLILEFEKTGDKAWTW
                     TARYGELDSDTGEIPSVGTGVVTFDENGAVVRGQDPSQDPAAPTITFTPAGGAADLKV
                     NLDFTALSQFGEACSVNWSTQDGYAAGTMETFNIDDRGIITGVYSNGQYKIIGQMVLA
                     SFANAEGLTRQANNMFAATTNSGSPQLGEATTGGRGSVMGTTLEMSNVDLAREFTDMI
                     ITQRGFQANSRVITAADEMLQELLALKR"
     gene            complement(9337..9750)
                     /locus_tag="PLU15_07625"
     CDS             complement(9337..9750)
                     /locus_tag="PLU15_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOO30830.1"
                     /translation="MTVDRTDMIAGRGMTPGISGVRPDRPAPAGTEVPLARGAGATFQ
                     DVLAEQLSPGGVKFSAHARSRLAARRIDLGQEDLSRLEGGVSRAAAKGARESLILLDD
                     KAFVVSVRNRTVITAVDGEHLKENVFTNIDSAVIV"
     gene            complement(9763..10167)
                     /locus_tag="PLU15_07630"
     CDS             complement(9763..10167)
                     /locus_tag="PLU15_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOO30831.1"
                     /translation="MYTSRAASVGTYAVPPENTPRKTLGKDEFLMLLITQMRNLDIGE
                     GSSNQEFVAQMAQFTLLEEVQNLSYSVQELMCRQLIAEAGSFVGKDIEAWIPGYDMPV
                     KGHVDSVEVIGGLPYLVVGDMRINMAYVTKIS"
     gene            complement(10182..13721)
                     /locus_tag="PLU15_07635"
     CDS             complement(10182..13721)
                     /locus_tag="PLU15_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOO30832.1"
                     /translation="MDGMKVGLAYKIPGELGGVGDGPDSARFLGLAAALDVGCNRGPG
                     GDARAPGSSLEFLWVLQEISSEMPGEAEAALTVARRLARCGHERADGQKDAPVLEGAA
                     RDETSVASRKVGAQALGKLRLDSTDDIRPAAEDGAQDEDEPDGESSVFTRTDLMEPVL
                     GPTVGVAVAIELPAPIVDTSRETNVPGDQTRGFRVGPARVDAARDSALGYEEGIDDAA
                     SVTDGFSELAMGNCDDGVVRAQLGRDVDPMESRDEHARSYASGDRVDGAVIREAKEGV
                     TTGEGGELAPAGPSELVMGEESTKSVQSDGMQCATMAGGDRVRSADSRRVDDVPSDGS
                     IQEAANGEGQAEPKSISGIPLMGGGADKIGHARIGAGSGFWSVVELDGRAALLDDVES
                     AMFLSGAMPVGSMGGRGRTVQMEMAERPAQRGGIEQAATSGKTPTVNVDRYHFRAGEN
                     GSGSHGAMMGGGTSGNRSRIWENVRTATPIQADDRGGTDGRKAAQTNVGVRRTHERGA
                     ASPKGPTGEGEASVEGAFGIDRADTGGAWERGVSTDAFFANLQGDDFQVDIADSEVGM
                     SAGATDEPAYLRNVSSAASEGAMAHTGTVDSLSELGRNAEMAITHLVAGQSEGDGQKG
                     ETMDESSSRADAHGEAVLQRRANQGQNSVRGVPDAESDETASLWKTEVSTADAFFSAI
                     ERDAISAEMRDSSPITARSFVGDVSEENARVGAMERPGDRVADGSGRIAAPTEDIAPK
                     SGNAVAGGFSADPEASLSAPSGGGVGLGMTSGVWGRGIDSRLAEERPMGVEQEGVSHG
                     SDGVDWAGTSNPKGIGGADEGKPERAELTEVESGAKARVGHGVSSRRHSEAETVVSTA
                     GSKLEARRIQTADSQSMAERATSDAGLIKSDGFGVRPAGRQNIVSGMDEGEESLAARE
                     EADGADAADGESVLRVRVPEPDVTMRRDNTSSDMQSDARSRERSHGSREVVFEPRRES
                     AQSGSEQIGHSDNGVVGFEGLVANLEAVNTGAAPTEGVPIDGRNRISDEAGRSMMRQI
                     AAHIEARRGVGSGEFRAKLVPDSLGEVRIRIRVQGGVCTATIRVGDSEVGEYIEEHGT
                     DLKVLLAESGIDLADLSVSVGSHSASEGAREWTQGQYAAAAFAGGGRRGRKDYAPVEE
                     WFCNSHRNELAPQEIELAAAYARSGHGRIDYFA"
     gene            complement(13816..14274)
                     /gene="fliJ"
                     /locus_tag="PLU15_07640"
     CDS             complement(13816..14274)
                     /gene="fliJ"
                     /locus_tag="PLU15_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOO30833.1"
                     /translation="MDTFTFKLQPVLDHRRRLEDQAKQEFAKAQSRELEARQVLADME
                     WNFGEGQSEMAEAARDDVDLAEVSACQRYLNRLRTAMLEQNDLVATLHLTSEEKRTGV
                     VDGMKARKIVEKLKEKEYEQYMVDINRLEQKQTDELATTRHGRSKKWTGL"
     gene            complement(14285..15628)
                     /gene="fliI"
                     /locus_tag="PLU15_07645"
     CDS             complement(14285..15628)
                     /gene="fliI"
                     /locus_tag="PLU15_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOO30834.1"
                     /translation="MCADHIASDLARDIDFSRYHAKLKGLDTIKQRGRVAQVIGLVIE
                     SDGPHAQVGEICSIYPRNATLPVTAEVVGFRENKLLLMPLGEMAGIEPGSEVVAGGNF
                     LTVRVGDELLGRVLDGLGRPMDGLGPIHTVSEYPVSSSPPNPLLRPRISKPLPLGVRA
                     IDGLLTCGCGQRVGIFAGSGVGKSTLLGMIARNTRADVNVIALVGERGREVLEFIEGD
                     LGEEGLARSVVVVATSDTPALVRIKGAMVATAIAEYFRDQGLNVMFMMDSVTRFAMAQ
                     REVGLAIGEPPATKGYTPSVFALLPKLLERSGTSERGTITGLYTVLVDGDDLTEPIAD
                     AVRSILDGHIVLSRKLADANHYPAIDVLRSVSRVMDNIVSPGQRAAAGRFRDLLATYR
                     EAEDLINIGAYVAGSNPKIDESIAAIDGFNAYLRQGVGEKVSFDEAVEGLITVVG"
     gene            complement(15618..16355)
                     /locus_tag="PLU15_07650"
     CDS             complement(15618..16355)
                     /locus_tag="PLU15_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOO30835.1"
                     /translation="MIKRTLIDDVLVASPAFCVPTDRPELSFVASDEEEFVPEADGEA
                     AIEQAEARAKEIIARSRREADLILKDAKAEAESIAKRAEKEGYELGQAAGLAQVREAY
                     AEALNTIDSVADSIVAAADALVSGCECEVAKLAVAVAEKLVRKTIDEDPEAVLCMVRE
                     AVRRTEGARTVVMRVSPADLEVIARAHNELIGLSPDLRSLEIVEDARIERGGCVIEME
                     VGSVDARIDEQLREVERSFLEEAGHVR"
     gene            complement(16357..>17353)
                     /gene="fliG"
                     /locus_tag="PLU15_07655"
     CDS             complement(16357..>17353)
                     /gene="fliG"
                     /locus_tag="PLU15_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOO30836.1"
                     /translation="AASGRRKAAIVLVSLDSETAASVLKHMKDDDIEQLTLEIATLGK
                     VTPEQQETALEEFYQLALAQEYIAQGGIDYAKQILEKALGTAKAMEIINRLSANLRVT
                     PFAFARNVSPEQLTGFIEGEHPQTIALVVAHLRPDQAALVLSALPPDLQADVALRVAQ
                     MEGARPEVIKEVEEVLERKLTTFGTQEFQAAGGVKSLVEVLQNVDRSTEKVILERLEE
                     ENPEIAEEIKNLMFVFEDIVFMEDRYVQMIIREIDSKDLALALKAASDEVKDKIMRNM
                     SERAATMLQEELEYLGPVRLRQVEEAQMKIVAVIRKMEEAGQIVAIREKGDEVIV"
     assembly_gap    17354..17453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17454..18868)
                     /gene="fliF"
                     /locus_tag="PLU15_07660"
     CDS             complement(<17454..18868)
                     /gene="fliF"
                     /locus_tag="PLU15_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOO30837.1"
                     /translation="MGIPDAGQDDIAQTEKSGLMSGLTKKQKIIVAAIVGGVFIVLIL
                     LIVSSSSKDYAVLYSGLSVEDAGRIVEHLKESGVKYRLTSGGHTIEVPEDRVYELRME
                     LAAKGFPQGAGAGFELFDKTSLGATEFTQKVNYTRALQNELARTIMTLTPVDQARVHI
                     VIPEERLFSEQQKPTTASIVLQMKPGAYLLDAQVRGVVHLASSAVQGLTPENVTVLDT
                     DGNLLWRASTAESTAGPMTTAQLEAKMGFEQEMQRRIETMLDRAFGLGKSIVRVYADI
                     NFDSAERASEIYQPLEGGLGIPVAVQTTQEERVGDSDAARGYAGTPSNVEGYPVLSGT
                     GSGESRYTRSESTTNYEVSKVVQKEIIAPGKVERVSVAVMLDRVMPMDRMEEMRQVIS
                     AAVGIQPERGDQIAITTMEFARAASEDEMFASAESLAKADKQAFIMRLVKSLTPLILG
                     LLVLLFAMSFLRVFREQQEYAT"
     gene            complement(18869..19171)
                     /gene="fliE"
                     /locus_tag="PLU15_07665"
     CDS             complement(18869..19171)
                     /gene="fliE"
                     /locus_tag="PLU15_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOO30838.1"
                     /translation="MSRIDGIGDGMALLGTPSVSSNDGTDLVRAFGDVLEQALDTVND
                     MQSRADRLTEKLAAGEVSDIHQVMIAVEQVNLALQLTMQVRNKIIESYQEVMRMQI"
     gene            complement(19401..19790)
                     /gene="flgC"
                     /locus_tag="PLU15_07670"
     CDS             complement(19401..19790)
                     /gene="flgC"
                     /locus_tag="PLU15_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOO30839.1"
                     /translation="MAPMSSFGVSASGMSAERLRLDAISNNLANINTTRTPEGGPYRR
                     IMVMLESSAGGVRVAGIERDSSPPMMVYDPGHPDAGADGCVAMPNVNPVLEMVDMITA
                     TRAYEANVAAFNAAKSMAAKALEIGKI"
     gene            complement(19794..20231)
                     /gene="flgB"
                     /locus_tag="PLU15_07675"
     CDS             complement(19794..20231)
                     /gene="flgB"
                     /locus_tag="PLU15_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOO30840.1"
                     /translation="MEFFESPTIKVLGAALDISSARHTAISRNVANANTPGYKAVDMD
                     FSETLKRSMAGAGAPRSVGVKATRPGHFGSRSHAATGAAGLSPVAVSSDPDGVSMRAD
                     GNSVDIDLEMAKMAENVILYNTVSHILASKFGMLRYVISEGRR"
     gene            complement(20438..>21653)
                     /locus_tag="PLU15_07680"
     CDS             complement(20438..>21653)
                     /locus_tag="PLU15_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO30841.1"
                     /translation="AGSFLSIISAFASAAIENAMLYRNLTEANEKLEVSRRYAERLIE
                     EAPLGMVVIDGECRVLTWNRALAEITNVCRGAATGKDLHDIITGSGSERLFALIDKAS
                     AEGDSTVAENIALHIEHGDDHIFRIIVSPVERLLGDNPGFIIMAEDVTEKVRLGEQLK
                     QAERLKTLGEFAAGVVHEINNPIGIICACAEVMEKKLSRHGPKMAECVKTAQIIQDEA
                     IRCSSIVKNLLGFARQGEINPEEVDVEEVLRQVVEFIKGYAAASNVEMDLKVSGDIPL
                     IIGDVLQLEQVFLNLSINAIEAMAGGGRLEVSACCAGDEVRISFSDSGPGIEGVAAQK
                     LFNPFFTTKSTGTGLGLSVSLGIVERHGGRIEVGDSDAGGACFTVVLPLTIDAWEDHL
                     PRSIGKHLHAGE"
BASE COUNT         4666 a         7000 c         4980 g         4907 t
ORIGIN      
        1 aaatcagcct cctgctgaat ctttctcctc cgcatcctag cctcttcctc ggttatcatc
       61 cctgcgttga ggtcggcatc gatgctcatc tgcttgcctg gcatggcgtc gagagtaaac
      121 cttgccgcaa cctcggccac cctgcctgca ccgttcgtga tcacgacgaa ctgtatgatc
      181 acgagaatga cgaagataat aaggcctacg atgtagtccc ctccgactac aaaattcccg
      241 aaggcgtcta tgacagaccc cgcatatcca cgcagaagta tgagcttggt tgcggagacg
      301 ttaagcgaca atctcaacaa tgtgctcaac aggagcagtg acgggaagac ggagaactgc
      361 agtggttcag tcatgtacat cgtgaccagc agaactccga gggagaatgc caggttgagc
      421 gcgagaagca cgtctaagac gattggcgga atgggaacta tcatcatcaa taggatgagc
      481 aataccatgg cggccatcaa caggtccccg ctcttcagat actgccgcag caacgtgcgt
      541 cctccgcttt gcccgtcagc catttctccc accccctccg tccaaaatca tcgggccttt
      601 gacagtctgt agacgaaagc caatatttcc gcaaccgccc tgtacagctc gggcggaatc
      661 tcctgcccta tctctactgc gctgaatatc gccctcgcaa ccggaggatt aggaacaaca
      721 ggaactcccg cctcctccgc aacctctcgg attcgctggg ctatgagacg ctgtcccttg
      781 gccaccacaa caggggcatt cattctctcc tggtcatatt taagggccac tgcatattct
      841 gtggggttca caaccaccac atccgcagtc ttaacatcct ccatcattcg cctcatcgcg
      901 atctggcgct ggcgttgcct tatcctggac ttgaccattg ggtcgccctc ttgctgcctt
      961 atctcttcct tcagctcctg cttcgtcatg cgcagattcc gctccagttc catcctttgg
     1021 tatgcatagt cggctacccc gattatgacg aacaagacgg acatttgctt caccacgtat
     1081 atccctgcgt ctgctgcagt agctaaccct tcgtcaatcg ccatgtaaga tgccatcgtg
     1141 atctgcggaa tcatggtccg cagcgcgaga taacccgaat agcctatagc gaccgccttc
     1201 acagccgact tgagcagtcc cacgagggct tccttggaga atatacgccc caaaccacta
     1261 ataggatcaa tcctgctgaa cttgggaacc aacagttttg gggaaaacat gaaccccacc
     1321 tgcgcaaggt tgaccacaag tcccacaaac atcgccccaa gcgcgaccgg cgctgcagcg
     1381 ataaggaagg tgatcatccc ttccatcaac aaactcattg cgcccacctg agaacaatct
     1441 gcgcttccca gaccgccaat ccttgtgatc atgaaattcg ccacggtctg ccccaaaaaa
     1501 gagaaagaag actcgagaat catgaggccg attatgaggc cgagtgcggc gttaaggtcg
     1561 gcgctccggg ccacctgccc ttcctttcgc gcctcctcac gcctgcgtgg tgtcggttgc
     1621 tctgtcctgt cctcatgaga aggtttagcc aatcgccctc acctcccaaa tccggcctcg
     1681 agctcctcca ctcgcccagc gcagctctgc cggctatgta cggtctccag acagcagact
     1741 agaatgccct catcagcacg acaatagcct ttcgcaagct cgcaaacgcc gtagcagcgc
     1801 acccaacaaa ccccgggatc gccagcccaa caactgcaag accaaccact atttttgcgg
     1861 ggaatccgac cataagcacg ttcatctgcg gaacggactt ggccagcacc cccaacgcta
     1921 tttcggttat gagcaagcaa gccactgggg gtagcgacat cctgatggca atcatgaaga
     1981 gatcggaaaa ccttgcaagc accagcgaaa aaagctcaga cgacatcccg gccccgccaa
     2041 tgggaatcac cgaatagcta cgcatcaggg caccgataag atagtggtgg ccgtctactg
     2101 ccatatacac catcattgcc acaaggtaat gaaactgccc cagcaagctg acttgctggc
     2161 cgaatgaagg atctactata tttgccaaac tgaagccgat ctgagtgtct atcatctcgc
     2221 ccccatactg agcggccgcg agaaccagaa gcgatacgaa cccaagcgtc gccccgacaa
     2281 aaagctctct ggccacgtct gacgtcaact catacacatc agtcggcatt tcgaaaggcg
     2341 tctgggccat catgggataa acaaccaatg cacatgcgaa agcaagccct gcctttgccc
     2401 gagcgggcac actggcgtgc ccaaaaagcg gtgccaggat gaacaagcct gtcatcctca
     2461 caagcaccag gaagaaaagc aaaaactccc tttctgtgaa tcccaatgtg ccttcagcca
     2521 ccttaagcac cgcctgtata gactactttg ccaacaaagg caacatagcg aagacctcgc
     2581 gcgcaaaccc cgtgagggtg ctggtcatcc acgatccagt cagaagcaat actgcagcga
     2641 tggccaacag cttaggaacg aaagtgagtg tgagctcctg tatttgcgtt gcggcctgca
     2701 gcactgatac gagaactccc actaccaatg cggtaaggag caccggcaaa ctgactttaa
     2761 atgccaccga caccgcctcc gacgccagtg cagcaaccat cgcctcattc atgttcaccc
     2821 ctccaaactc ccaccatgct tacctggcca cactttccac caacgacttc acaaccaatc
     2881 cccatccgtc caccagcaca aacattaaaa ccttgaatgg cagcgaaacg aggaccggcg
     2941 ggagcatcat catccccatc gacatcaaaa cgctcgcaac gagcatgtca atgacgagaa
     3001 atgggatgaa tatgacgaag cccatttgaa atgccgtctt gagttcgctg accatgaatg
     3061 ccggcattat cacataggtc ggtatgtcct ccggcccgcc cggctttggc aatccggcaa
     3121 gatccacgaa gagcgcgagg tctttctccc gcgtctgctt gagcataaaa ctgcgcaagg
     3181 gtgctacccc gcgggccatg gcttcctcct ggcttatgat gccggccatg taaggcttca
     3241 atgcctcctc atttatctga gaatagacag gcatcatcac gaacaaggtg acaaagaggg
     3301 ccagcccgat caggacttgg ttcggcggca cctgttgcgt gcccatagca tttcggacaa
     3361 atgagagaac aatgactatt ctggtaaacg aagtcgtcat caagagtatt gccggcgcaa
     3421 tcgacaatac cgttagcaga gcgataatcc gcagtgctcc cgctacttct gtcgggctct
     3481 ccgcggcgtc cacccctatc cttatgttcg ggataggcat gggctggcag taggccactg
     3541 ccgaacatgc aagcgctatc cctattcccg ccataatcgc ccctatccaa acaagcgctg
     3601 tccttgctga gcggggtatc cgagtctcca accgggcgcg tctcttgttc atacagcgtc
     3661 caccccgcct tcgcttcctc caccatcctg gctgtcggtg ctctcctcat cggtttggcc
     3721 atacagggca gcgctcaagc ccccatcgcc atcaggcatg tcccctgttc cctcttggcc
     3781 actgccgcgt ggccattgaa caatactgat cccgccacgc cccacgccta tcagcgcaat
     3841 cttgccatcc acttcagcaa ggtgcagata ggaatcggcg cctaggcgag cggtctcaat
     3901 gaggcggatg cgcctttccg ggcctgatac ctttatcatc cccgacgcac gagccttctt
     3961 gagaagccaa gccacaagat aaatgaggcc cgcaaccgct gcgagggcaa gcgcagcgcg
     4021 caggagcaac atggccatac tctcttccat tcctattgca ctgctttctc cggtgccttg
     4081 acgaactcca cgatcctcac cccgaaattc tcctcaatca ccacaacctc gccccgggca
     4141 atcagccgac cgttgatgag gatgtcaaca ttctctcctg ccgccttgtc aagctcgatt
     4201 atggaccctg gcccgacaga cattatgtcc ttcacaagca tggtagtccg cccgagctcg
     4261 accatgaccg gaagcggcac atccatgagc aggtccagat tgtcgccttt cccgccttcc
     4321 atgccgggtt cgtcaaatgg catgaactgg acgggctgta catttgcctg ttcctcctgc
     4381 acctgcttgg atgatctcac tgcagactga gccgactggt ttgagtgtga cgcctccgga
     4441 acctcaggcg tcagatcaaa ctgaggaaca gcatcatcat cagtctgcat gtcgtcccac
     4501 gagaattcac tcatgacatt cccctcattt ctacgtagac tctacagtgc caacgatctg
     4561 gattgcgtaa ttcttcccga caagccccgg aatcgcatga aacttcgggc tgtttccgat
     4621 cacaactgtg agagggtcga aggcatgctc atccagttta ataacatcgc ccgtctgcag
     4681 ttccagcatc tcgctgacgg tgatcgacgc ccttcccaaa caggccgcaa tgggaacctt
     4741 tactccctcg agcccagcgc tgatggcgtt ggccgacttt tcggaactga tcttggtgcc
     4801 tttctgatac cactgctggg cactgagctt ctgaagcaca ggctgcaacg tggaatgcgg
     4861 catgcaaatg ctcatggagc cattggcttc tcccaccctg atctcgaatg agatgacgca
     4921 cgtggtatcg tttggcgaaa ctatctgcgc gaactgtgga ttggattcat atgcatcgat
     4981 atggggatcg aatctcacca taggatccca agcgtctttc agatcgtcca tggcccgatc
     5041 caccatcgcg cggacaagct tctcctctat ttcagtgagc tcgcgcactc gtccccgtac
     5101 acgtccgggg ccgcccatga gcctgtctat cattatgaaa actatgtacg ggttaaattc
     5161 tattatggcc tgtccgtcaa ggggctccgg tgtaaagaca tacatgactg ccggcgactt
     5221 cagagagctt aggtattcat cgtatatacg ctgttgtaca tcgatgagcg tcaccttcac
     5281 cggcgtgcgc aaatgcgctg aaagcgtcgt tgagaggtag cgcgcgaagg tctcgtggag
     5341 catgtgaatt gttctcagct gctcacggga gaatctgttc ggacgcttga aatcataaac
     5401 cgtgtagctg acaccatccg acagagcccc acgatgttcc tgatcctctt cctcgctttt
     5461 cgaaaactgc gcgaggagcg catttatctc ctcttgtgtc aaaacttcgg ccaatgaaac
     5521 caactcccgg atctattgga caacaaattc actgaaaaat acccttttca catctgcttt
     5581 cgcaataagg cgggcgacgg cctcgactaa ttcggctctc aacgcgtccc ttggctcgcc
     5641 cggttcgagc tcctccaagg tcttggatga aagcacatct atgatcgcat cgcgaagtag
     5701 cggcaccttt cccgccgcct ccgttactcc cgctttatca tcgaactcca cagtaagcgt
     5761 gcacttcagg aaacggtggc cattggtatc agctatgttt actataaacg tgtcaaaggt
     5821 gtactcgggg ccaatctcgt ctcgatccgc gttcgtctgt tcggtctgcg ccatctcaga
     5881 cgcgccgctt tccgtgcccg gcgcccgtcg cgcaacaaga cagtatccga ggattgctcc
     5941 gaccagcagt gccgcaactg caaacgtcgt cttccgcttg cttttgaact ttctcttgtt
     6001 gccattctca gcttttttca ttccatcaca tccccggaat atcagtcaca tctgacgcaa
     6061 taatgagtat atcgacacgc ctgttcaatt ccctgtttgc atatgatgta ttcggaagca
     6121 cggggcggta ctcgccataa cccgcagccg aaaaccgctt cggggatagt cccagttcct
     6181 caataaaata gcgaacgaca gtcgtcgcgc gcgccgtaga aagctcccag ttggtctcgt
     6241 attgggctcc acggcggagc ggcacattgt cggtgtgccc ttcaactacg acctcattct
     6301 gtatgggccc gatgaacttc gccagatcgc gcagaagcat cctggagtcg ggcctcagtg
     6361 tcgccatgcc caagtcgaac aggaccgtgt ctgtgaatct gatcagaagc cccctcttgt
     6421 tcatctcaac gtggaccttg ccgcgaagcc ccttttcttc gagatactct tccaactggc
     6481 ccttgatgat caccagatcc gtgcgggcct gcgacatggt atcgtcacct atctgcgacg
     6541 ggtcatccgg aggaatgggc atctctcccg gctccaataa agccgggccc ccgctcaata
     6601 cgcccaacgc gccctggaga gaaatcaacg cctctctgaa cctttgcatg tcgatcgaag
     6661 agaaagcaaa gagcatgatg aagaaggtca gaaggagcga catcaggtcg ctgtaggtcg
     6721 tcatccaaga aggctgcgac cctcccgact catcatttct cttgcgcctc ctcatagcgt
     6781 tgtgccactc cctcctgtgt cttccgccat ctcccgccgg gacctggcgc ctccgcgcct
     6841 catcccagcc cgttcttgag ctcgcagtct atccttgggt gccaaaaacc ccatgagctt
     6901 ctcttctacg atacgcgggt tctcgcccga ctgtatcgag aggattcctt cgagcatcat
     6961 ctcacgcaag cgtatctcct cggcgctttt cacaccaagc ttagtagcga taggacctgt
     7021 gaccaagttg cctatcacag cgccgtacaa agtggtgata agagccaccg ccatggcggg
     7081 gcctatggtg ctgggcttat ccagagtgcg aagcataaga acgaggccga tcaatgtccc
     7141 gatcatcccg aaggccggag catatgccgt catggcgtcg aatatggctt tgccgtttct
     7201 atgcctgtcc tctatatagg caaggtcagt ctctagaata tctcgcacca gttccggatc
     7261 tgtgccgtcg acaattagct gaattcctct ttgcaaaaac gggtcgtccg tcctgttcag
     7321 gctgtcttca agggccagga gcccctctct tcgcgccatt tccgccagat caaccattcc
     7381 atcgatgatg accaacggat cctcctcttc agacctgaaa gcgatcttta tcatcttggg
     7441 gatatctttg agctgctccg ccgaaaagct gattatcaca gcacccagcg ctcctcccac
     7501 agttatcagg accgattgaa tatcccaaaa cggcctgagc ccagcatcgc tttgcatgag
     7561 cgctatgaga atacacgcta ctcccaatat aaaccctata acagtcgcac tatccatttg
     7621 cctacctcgt ctgcttctcc gctgaatccc tgacaatcac tgcccgacgg ttaataaggc
     7681 ggtgatagcg gataatcatg tcaaccactt tctccactga ttcttccact atgagtttgg
     7741 tgccattagt gagtgtaatc acagtatcgg gggtagcggc tatcgtctcg atcaactccg
     7801 cattaatatg tatcggcgat ccgttcaatt tggttacctt tatcattcaa caaggccccc
     7861 ttgtacacta ctcgcatcag cagaggcaca ggggggagag ggctagactc tgcccgatcc
     7921 ccctctcccc gcctcaatgc ttcttgccgg cacgtgtaac gtcacaacct gccaacgatt
     7981 atctcttcag tgccaaaagc tcctgaagca tctcgtctgc ggcagttatc accctcgaat
     8041 tagcctggaa accgcgctgg gttatgatca tatccgtgaa ctcccttgca agatcgacgt
     8101 tggacatctc aagagtcgtc cccattacag acccgcgccc gcctgttgtg gcctcgccaa
     8161 gctgcgggct gccggagttc gtcgtagccg caaacatgtt gtttgcctgt cttgtcaggc
     8221 cctccgcgtt tgcaaaggag gccagcacca tctggcctat gatcttgtac tgtccgttgg
     8281 aatatactcc cgtgattatc ccccggtcat cgatattgaa cgtctccatg gtgcctgcgg
     8341 catagccatc ctgagtgctc cagttaacag aacacgcttc cccaaactgg ctcaacgccg
     8401 taaaatccag gttcactttg aggtctgctg caccccccgc aggagtgaac gttatcgttg
     8461 gggctgctgg gtcttgactt ggatcttggc ccctcaccac ggcgccgttt tcatcaaaag
     8521 tcaccacgcc cgttcctaca cttggaatct cgcctgtgtc ggaatcgagc tctccatatc
     8581 tggcagtcca cgtccacgct ttgtccccgg tcttctcaaa ctccaatatg agcgtgtgct
     8641 gaatgccaag tgagtcatag atagggacag aagtgctgtg caaggttcca acatcgcctt
     8701 cggcattgag gtttccgaca aacgtgattc tgtccgtagc tttggcactc atgctggttc
     8761 ccagcggaat tctgagtccc tgtatctcac cgctccggtc gattgtccct ttggcatcgg
     8821 ccatccatcc ctgcaccatg aaaccagtgg acggatgggt gaggtttcct tcagcatcta
     8881 tcttgaacgc tccatccctc gtaaacatca agcctccact ggaatcgcgg accacaaaca
     8941 atccgctgcc ttggatggcc atatcagtca gccttccagt gtattccagg tttccggggg
     9001 taaacacgat atcgatcgac cttgtggaaa cccccatgcc aacctgcatc ggatcgaccc
     9061 ctcccctgct tccagtaggc gccattgaac ccctcatggt ctgggagagc atgtcctgga
     9121 aggttgccct tccatacttg tatccaacgg tgttgacatt ggctatgtta ttgccgatca
     9181 cgtccatgcg cacctgatga gcgcgggttc cggacacgcc ggcaaacatt gaacgcatca
     9241 ttcacgttcg acctccatat ctcttttttc cgcaggccgt ctcagtccca gacggtccag
     9301 ggcttcctca tagaggtccg gcccaaatta gcggatctag actatcaccg cagaatcgat
     9361 attggtaaac acgttctcct tgaggtgttc gccatcgact gcagtgatga ctgtgcgatt
     9421 tctcacgctt acgacgaatg ccttgtcgtc caaaaggatt aaagattctc gcgcaccctt
     9481 ggcagctgcc ctgctcacgc caccctccag cctcgaaagg tcctcttgtc ccagatctat
     9541 cctgcgggcg gccagcctgg agcgtgcatg agctgagaat ttgactccgc ctggagacag
     9601 ctgttctgcg agcacatcct ggaaggttgc tcccgcgccc ctggctaagg gcacctcagt
     9661 tcccgctggc gccggcctgt ccggtcgcac cccggagata cccggggtca taccgcgacc
     9721 agctatcatg tctgtcctgt ccactgtcat ctgtcgacac ccctaagata tcttcgtcac
     9781 atacgccatg ttgatccgca tgtccccaac taccaggtaa ggcaatcctc cgattacctc
     9841 caccgagtcc acgtgccctt tgacaggcat gtcgtagccg ggaatccatg cctcgatatc
     9901 ttttcccaca aaggacccgg cctctgcaat cagctgcctg cacatgagtt cctgcactga
     9961 ataacttaga ttctgaactt cctccaagag ggtgaactgg gccatctgcg ccacgaactc
    10021 ctgatttgaa gatccttcgc ctatgtcaag gtttctcatt tgtgtaatca gaagcatcag
    10081 gaactcgtcc ttacccaggg ttttcctggg agtgttctcc ggaggaaccg catatgtccc
    10141 tacgctcgca gctctggaag tgtacaatcc tcatcccccc ttcaagcaaa gtagtcgatc
    10201 cttccatgcc cggatcttgc ataagccgca gcaagctcga tctcctgcgg cgcgagctca
    10261 tttctatggg agttacagaa ccactcttca acaggcgcgt agtccttgcg tcccctgcgc
    10321 ccgccgcctg cgaaagccgc ggcagcgtac tgtccttgcg tccactcccg cgcgccctca
    10381 gacgcagagt gcgatcccac cgatactgag aggtccgcca gatcaatgcc actctctgcg
    10441 agaaggactt tgagatctgt cccatgttct tctatgtact ccccaacttc actatcgccc
    10501 acgcgaatcg tcgccgtgca gactccgccc tggaccctta tccttatccg aacctctcca
    10561 aggctgtcag gaacgagctt cgccctgaac tctccggatc caacgccgcg cctggcttca
    10621 atgtgggcag ctatctgccg catcatcgaa cggccagcct catctgatat gcggtttcgt
    10681 ccatctatgg gcacgccttc tgttggtgcc gcgcctgtgt tgactgcttc caagttcgct
    10741 accagccctt caaacccaac aactccattg tcggaatggc cgatctgctc agatccggat
    10801 tgtgccgact cccgccttgg ttcaaagacg acctcgcgac tgccgtgcga tcgctctcgc
    10861 gaacgggcat ccgattgcat gtccgagctt gtattatccc gcctcatagt cacatccggc
    10921 tccggtacac gaacccgcag tacactctcg ccatcagcag catcggcgcc atccgcttcc
    10981 tccctcgctg ccaggctttc ctctccctcg tccatcccgc ttacgatgtt ctgacgccct
    11041 gcaggtctca ccccaaaccc atcggatttg attagcccgg catcggatgt cgctctctct
    11101 gccatcgact gagagtcggc tgtctgaatc cgccgagcct caagttttga tcccgcagtg
    11161 cttaccacgg tctccgcctc actgtgacgt cgagaagaaa ccccatgtcc aactcgtgcc
    11221 ttggcacccg attcgacctc tgtcaactcg gccctttccg gcttcccttc atcagcgccc
    11281 ccgattcctt tgggattaga cgtgcccgcc caatcgactc cgtcagagcc atggctcacg
    11341 ccttcctgct ccacgcccat gggcctttcc tctgctaaac ggctgtcgat gcctcggccc
    11401 cagaccccgc tggtcattcc caggccgaca cctcccccgc tcggagcaga aaggctggct
    11461 tccggatccg cggaaaaccc gccagcaaca gcattacccg atttcggggc tatgtcttcc
    11521 gttggggctg cgattctccc gctgccatcg gcgactcggt ctccaggacg ctccatagcg
    11581 cccacccgcg cgttttcttc agacacatcg cctacgaacg atctcgccgt gattgggcta
    11641 ctgtcccgca tctcagccga aatcgcgtct ctttcgatgg ccgaaaagaa tgcatcagct
    11701 gtcgatacct cagtcttcca gagactagcc gtttcatcgc tttcagcgtc gggaaccccc
    11761 cgaacagagt tttgcccttg gtttgcacgc ctttgcagta ctgcctcgcc atgcgcatcc
    11821 gcacggctag agctttcatc cattgtctcg cctttctggc catctccttc ggactgaccc
    11881 gcaacgagat gagttattgc catctctgca ttccggccca actcgctgag gctgtccact
    11941 gtcccagtgt gtgccatagc cccttcgctt gctgcactgc tcacattgcg aagataggca
    12001 ggttcatctg ttgcccccgc actcattccg acttcggaat ccgctatgtc gacctgaaag
    12061 tcatctcctt ggaggtttgc aaagaacgcg tccgtcgaca ctcctctttc ccaggctccg
    12121 cccgtatcgg cgcggtcaat gccgaaggct ccttcgacag atgcttctcc ttcacccgtc
    12181 ggcccttttg gggatgcagc accgcgctca tgcgtgcgcc tgacgcctac gttagtttgg
    12241 gcagccttac ggccatcagt tccccctctg tcatcagcct ggataggcgt tgccgtcctc
    12301 acattctccc atatcctact gcggtttcca gacgtgccac cgcccatcat ggccccgtga
    12361 gatccactgc cattctcgcc agcgcggaaa tggtatctgt ctacattcac cgtcggcgtt
    12421 ttcccgctgg ttgctgcctg ttcgatgccc ccgcgctgag ctgggcgctc cgccatttcc
    12481 atctgcacag ttctgccccg tccacccatg ctcccaacag gcatcgcgcc cgataggaac
    12541 atggcggact ctacatcatc cagaagagcc gcgcgaccgt caagttccac aacgctccag
    12601 aagcccgacc cggcgccaat ccgggcatgc ccgatcttgt ctgctcctcc gcccatcagc
    12661 ggaatgccag atatagactt gggttctgcc tgaccctcac cattagctgc ctcttgaatg
    12721 gagccatcgg aaggaacatc atccacccgc ctgctatctg ctgaccgaac ccggtcaccg
    12781 cctgccattg tggcacattg cataccgtct gattgtacag acttcgtcga ttcctcgccc
    12841 atgacaagct cgctcgggcc agctggcgct agctcgccgc cttcgccggt tgtgacacct
    12901 tccttggcct cacgtatcac cgcaccatca accctatctc ctgaagcata agatcgagca
    12961 tgctcgtccc tgctttccat aggatctaca tcccgaccta gctgggcccg gacaacgcca
    13021 tcatcgcaat tgcccatagc aagctcagaa aacccgtctg tgaccgatgc cgcatcatcg
    13081 attccttcct catatccaag cgcgctatca cgcgccgcat cgacgcgtgc gggtccaaca
    13141 cgaaagcccc gtgtttgatc accaggaacg ttcgtctctc gcgatgtgtc aactatcggc
    13201 gccggtaact ctattgccac agccactccc acagtcggcc ctaaaaccgg ctccataaga
    13261 tccgtcctcg tgaaaacgga actttcgcca tcaggctcgt cttcatcctg cgcgccatct
    13321 tcagctgcag ggcggatatc atcggtcgaa tccaacctca gctttccaag agcctgagca
    13381 cccactttcc gggacgcgac agacgtctcg tctctggccg caccctccaa gacaggagca
    13441 tccttctgtc catctgccct ctcatgcccg cagcgagcca ggcgccgggc gaccgtcaat
    13501 gcagcttctg cttcgcctgg catttccgaa gagatctcct gcagcaccca gaggaattcc
    13561 agtgaactgc caggcgccct cgcgtcccca cccggtccac gattgcagcc cacgtccaaa
    13621 gcggccgcaa gccccagaaa gcgggctgaa tccgggccgt ctcccacgcc tcccagctcg
    13681 ccagggattt tataggcaag acccaccttc ataccatcca tcttgtctat cacctccctt
    13741 caccctcata tccaacgtgt ccagggcgct ggcttttcac ctttgtccct gccgcatgcg
    13801 gcagtcagcc aataatcaga gccccgtcca cttctttgac cttccatgcc ttgtagtggc
    13861 caactcatcc gtttgctttt gctcaagacg gttgatatcg accatatact gctcgtactc
    13921 cttctccttg agcttctcca ctatcttgcg ggccttcatt ccatctacaa cgcccgtcct
    13981 cttctcttca gacgtaagat ggagcgttgc aacgaggtcg ttctgctcca gcatcgcagt
    14041 tctcaatcga ttcaagtatc gttggcacgc ggagacctct gccaaatcga catcgtcccg
    14101 agcagcctca gccatctcgg actgcccttc cccgaaattc cactccatat ccgccaatac
    14161 ctggcgggcc tcgagctcgc gcgattgggc cttggcaaat tcctgctttg cctggtcttc
    14221 caggcgtctt ctatgatcca ggaccggttg cagtttgaaa gtaaaagtgt ccatgccgat
    14281 ccccctatcc cacaactgta atcaaacctt ctacagcctc gtcgaacgaa accttctccc
    14341 ccacgccctg cctgagatag gcattgaacc cgtctattgc ggcaatcgac tcatctatct
    14401 tcgggttcga tcctgccaca taggccccga tatttatcaa atcctccgcc tctcggtatg
    14461 tggctagtag atcgcggaac cttccagcag ccgcccgctg cccaggagag acaatattgt
    14521 ccattactcg gctgacgctc cggagcacat cgatggccgg gtagtggttg gcgtcagcaa
    14581 gtttgcgcga aaggactatg tgtccgtcaa gaatgcttcg aactgcgtcg gcaatcggtt
    14641 cagtcaggtc gtcaccgtcc acgagcaccg tgtaaagacc ggttatcgtc ccccgctcgc
    14701 tagtccccga gcgttcaagc aattttggaa gcagcgcgaa gacagaagga gtatatcctt
    14761 tggtcgccgg cggctcgccg atggccagcc caacctcgcg ctgagccatg gcaaaacggg
    14821 ttaccgaatc catcatgaac atcacattga gcccctggtc acggaagtat tcggcgatcg
    14881 cggtagccac catggctccc tttattctga ccagcgctgg agtgtccgaa gttgccacaa
    14941 ccaccacaga cctggccaat ccttcttccc cgaggtctcc ttctataaac tcgaggacct
    15001 cgcgtccgcg ctctcccacc agcgcaatca cgtttacatc ggcccttgta ttcctggcga
    15061 tcatgcccaa cagagtactc ttgcccaccc cgctccctgc gaagattccc acacgctgcc
    15121 cgcagccgca cgtgagcaat ccgtctatag ctcgaacccc gaggggcagc ggcttcgaaa
    15181 tcctcggcct caaaagggga ttcggtgggg aactcgacac agggtattca gacacagtgt
    15241 ggatcggccc caatccgtcc atgggcctcc caagcccgtc cagaaccctc cctagaagct
    15301 catcacccac gcgcacagtc aggaaattgc cccccgccac gacctcgctt cccggctcaa
    15361 ttccagccat ctctcccagc ggcatcaaga gaagcttgtt ctcacggaac cccaccacct
    15421 cagccgtcac aggcaaagtg gcgtttcgag ggtatatgga gcaaatctct ccgacctgcg
    15481 catggggccc gtctgattcg atcacaaggc caataacctg tgccactctt ccacgctgct
    15541 tgatagtgtc aaggccttta agctttgcat ggtatctact aaaatctata tccctggcca
    15601 gatcggacgc aatgtgatca gcgcacatgt cctgcttcct ccaagaagga tcgctccacc
    15661 tcgcggagct gctcatctat tctggcatcc accgacccaa cttccatttc aatcacacag
    15721 ccaccacgct cgatcctggc gtcttcaaca atctccaggc tgcgaagatc gggggaaagt
    15781 ccgatcaact cgttatgtgc ccgggcaatc acctcaagat cagcaggtga aaccctcata
    15841 accactgtcc tggccccttc agttcttcgc accgcctcac ggaccatgca aagaacagct
    15901 tccggatcct catctatcgt tttgcggaca agcttctcag ccactgccac agcgagcttc
    15961 gccacctcgc actcgcatcc gctcaccaag gcgtcggccg ccgctactat tgaatcggca
    16021 acagaatcta tcgtgttcag agcctcagca taggcctccc ggacctgggc aaggcccgca
    16081 gcctgcccca actcgtatcc ttccttctca gcccgctttg caatactctc cgcttcagcc
    16141 ttggcatcct taagaatcag gtccgcctcc cgccgagatc tggcgatgat ctctttggcc
    16201 cgggcctcag cctgctctat cgcagcctct ccgtccgcct cgggcacgaa ttcttcctca
    16261 tccgaggcga caaaggatag ttcaggcctg tcagttggca cgcaaaatgc gggagaagcg
    16321 acaagaacgt cgtctatgag tgtccgcttt atcaccttag acaatcactt cgtccccctt
    16381 ctcgcgtatg gcgacgatct gcccggcctc ttccatcttt cttatgactg cgacaatctt
    16441 catctgcgcc tcctcaactt ggcggagcct tacaggaccg agatactcca actcctcttg
    16501 gagcattgtt gctgcccttt ccgacatgtt ccgcatgatc ttgtccttca cctcatccga
    16561 agcagctttc aaagccaaag caaggtcctt ggagtctatc tccctgatga tcatctgcac
    16621 gtagcggtct tccatgaaca cgatgtcctc gaagacgaac atgagattct tgatctcttc
    16681 agcaatctcc gggttctctt cctcgagcct ttcgagaatg accttttcag tggagcggtc
    16741 aacgttctgc aaaacctcca ccagcgactt gacgccgcca gcagcctgaa actcctgcgt
    16801 tccgaaggta gtcagttttc gttcgagcac ttcctcgact tccttgatca cctcgggcct
    16861 tgcgccctcc atctgggcca cccgaagcgc cacatccgcc tggagatccg ggggaagcgc
    16921 cgacagaacc agcgccgcct gatctggtct gaggtgggca acgaccaagg ctatagtctg
    16981 cgggtgctcg ccctctataa agccggtgag ctgctcaggg ctgacattgc gcgcaaaggc
    17041 aaaaggcgtc acgcgcagat tggccgaaag cctgttgatg atctccatgg ccttagccgt
    17101 acccaacgcc ttctccagaa tctgtttcgc gtaatctatt ccgccctggg ctatgtactc
    17161 ctgagcgaga gccagctggt aaaactcctc cagggcggtc tcctgctgtt caggcgtgac
    17221 cttgccaagc gtcgctatct ccagcgtcag ttgctcaata tcatcgtcct tcatgtgctt
    17281 gagaacggat gcggcagtct cagaatcgag agacaccagg actatagctg ccttccgcct
    17341 tccgcttgcc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttgcat
    17461 attcctgctg ctcacggaag actcgaagaa aagacatggc aaacaggagc acgagcaatc
    17521 cgaggatgag aggggtaagc gacttgacaa gacgcataat aaaggcctgc ttatccgctt
    17581 tcgcaagact ctccgctgat gcaaacatct cgtcctcgct cgcggcccgc gcaaattcca
    17641 tggtggtaat ggctatctgg tccccccgct ccggctgaat acctaccgca gcgcttatca
    17701 cttgccgcat ctcctccatg cggtccatcg gcatcacccg atcgagcatc acagcgacag
    17761 agaccctctc cactttgccc ggagcgatga tctccttctg aacaaccttg gacacctcgt
    17821 aattagtcgt gctttcgctt ctcgtatacc tcgattcacc gctgcccgtc cccgaaagaa
    17881 ccggataacc ttcgacattc gacggcgttc ccgcatagcc ccgggcggca tccgaatcgc
    17941 caacccgttc ttcctgagtc gtctggacag ctaccggtat ccccaggccg ccttctaaag
    18001 gctggtagat ctcgcttgcc cgctctgcgc tgtcgaagtt gatgtcagcg tatacccgca
    18061 ctatcgactt gccaagtccg aaagcacggt cgagcatcgt ctcgatccgt ctctgcatct
    18121 cctgttcaaa ccccatcttg gcttcaagct gcgccgtagt cattgggccg gcagtcgatt
    18181 ccgcagtact ggctcgccaa agcaaattcc cgtctgtatc gagcacagtc acattctcag
    18241 gggtaaggcc ctgcacagca ctggacgcca gatggaccac accccgcacc tgagcatcga
    18301 ggagatatgc gccgggcttc atctgaagga ctatcgatgc tgtcgtgggc ttttgctgct
    18361 ccgaaaagag cctttcctcg gggatcacaa tatggaccct cgcctgatct acaggggtaa
    18421 gagtcataat cgtcctcgcc agctcatttt ggagggcccg ggtgtagttt accttctgcg
    18481 taaactcagt cgcccccaga ctcgtcttat cgaacagctc aaatccggcg ccggcgccct
    18541 gtgggaaacc cttcgccgcc aactccatcc tcagttcgta gacacggtcc tctggaacct
    18601 cgatagtgtg ccccccagaa gtcaacctgt acttcacgcc cgattccttt agatgctcga
    18661 cgatcctgcc ggcgtcctca acagacaagc cggaataaag tacggcgtag tcctttgagg
    18721 agctggatac aatgagaaga atcaggacga tgaatactcc cccgacaatt gccgcaacta
    18781 tgattttttg cttctttgtc aaaccggaca tgagcccaga cttctcagtc tgagcgatat
    18841 cgtcctgacc tgcatcggga atacccattt atatctgcat cctcattact tcctgatatg
    18901 attcgatgat cttgttcctt acctgcatgg taagttggag agccaagttc acctgctcca
    18961 ccgcgatcat cacctgatgg atatcgctga cttcgccggc ggcaagcttc tcagtgagcc
    19021 tgtcggcacg ggactgcatg tcattgacgg tgtcgagcgc ctgctcgagc acgtcgccaa
    19081 aagcgcgaac caaatctgtt ccatcattgg acgacacgga cggcgtccca agcagcgcca
    19141 tgccgtcgcc tatcccatcg atcctcgaca tccgcatgca cctccatgcg cctgcttcaa
    19201 gattcccttt ccatctgagc aaccctcagt caaactctgt gccgcctgta ttttgcattt
    19261 tgcgaaatcc agagcagagc ttttccacac tactcaagaa tgttctcaaa atcagtctgg
    19321 cagcttccag tatctcttcg cgccatccaa aatccacgtt caagctcccg gatagcgttc
    19381 aggcactcgt cgaaaaccta ctatatcttc cctatctcga gcgctttcgc agccatagac
    19441 ttggcagcat tgaacgcagc aacattcgcc tcgtatgccc gggtagccgt aatcatgtcc
    19501 accatctcca gcaccggatt gacattaggc atggccacac agccatcggc cccggcatct
    19561 ggatggcccg gatcatatac catcatcggc ggcgaactgt cccgttctat tcccgccacg
    19621 cggactccgc cagcagacga ttctagcatc accatgatcc gcctatatgg cccgccctcc
    19681 ggcgtgcgcg tggtattgat attcgcaaga ttgtttgaaa tagcgtcaag acgcagcctt
    19741 tcagcggaca ttcccgacgc gctcaccccg aaactcgaca tcggcgccat tcattaccgc
    19801 ctcccctcgc tgataacata cctaagcatc ccgaacttgc tggccaaaat gtgcgacaca
    19861 gtgttgtaca agatcacatt ctccgccatc ttagccatct cgagatcgat atccacactg
    19921 tttccatccg ccctcatgga aaccccgtca ggatcagacg acactgccac cggcgaaagc
    19981 cccgccgcgc cagtcgccgc atgagaacga gatccgaagt gccccggccg cgtagccttt
    20041 accccaacag aacgaggggc gcccgcgcca gccatggacc gcttcaatgt ctcgctgaaa
    20101 tccatgtcga ccgccttgta tcccggggtg ttggcgttcg ccacattgcg cgagatagcc
    20161 gtgtgcctgg cggaggagat atccagggcc gctccgagca cttttatagt cggactttca
    20221 aagaattcca cggaccgaag cctcctttcg aaaaggaaat acgaaatcca ataaatatag
    20281 atgcaataat gatgccagcg aaacagaaca aaagagcctt tccgcaaacg gacgtggaaa
    20341 ggctatgtaa gctggcaata tgttcctcat ccagtttcaa gccgaaagtg gcgccagcgc
    20401 aaacggggac ccgctcattt tctgaggtga gcaattttta ctcacctgca tgcaagtgtt
    20461 tccctatgct ccggggcaaa tggtcctccc aagcatctat agtgaggggg aggaccacag
    20521 tgaaacacgc gccgccagca tcagaatctc ccacttcgat cctgcccccg tggcgctcca
    20581 cgataccaag gctcaccgac aatcccagac ctgtgccagt cgacttcgta gtgaaaaaag
    20641 gattgaacag tttctgggca gctacgccct caattcccgg tcctgaatca gaaaaggaga
    20701 tccttacctc atcccccgcg cagcaggcag atacctccaa ccggccacca cctgccatcg
    20761 cctctatggc gtttatggac agattaagaa agacctgctc aagctggagc acatcaccga
    20821 ttatcagcgg gatatccccg cttactttca aatccatctc tacattggaa gcagccgcat
    20881 agcccttgat gaactcgaca acctgcctaa gtacctcttc gacgtctacc tcttcagggt
    20941 taatctcgcc ctgtctggca aagccgagga ggttcttgac tatggaagaa caccttatag
    21001 cctcatcctg tataatctgg gcagtcttta cacactcagc catcttgggc ccgtggcggg
    21061 agagcttctt ctccatcacc tcagcacacg cacagattat gcctataggg ttgtttatct
    21121 cgtgcactac ccctgcggca aactcgccca aagtcttcaa tctctccgcc tgcttaagct
    21181 gctctccgag gcgaaccttt tccgtcacat cctcggccat gattataaaa ccggggttgt
    21241 cgccgagaag acgttcgact ggggagacaa taatccggaa aatgtgatcg tccccatgtt
    21301 caatatggag ggcaatgttc tccgcaacag tggaatcccc ctctgcagat gccttgtcaa
    21361 tcagggcgaa tagtcgctca ctgccggatc ccgtgatgat atcgtgaagg tccttccctg
    21421 tggcagcccc ccgacagaca ttagtaatct cggccaacgc cctattccaa gtaagcacgc
    21481 ggcattcacc atctatcacc accatgccta aaggggcctc ctcaatcagt ctctccgcat
    21541 atcttcgcga gacctcgagt ttctcattgg cctcagtcag attccgataa agcatagcat
    21601 tttcaatcgc tgcactagca aaagcagata tgatagacaa gaagctgcct gcg
//
LOCUS       DAOSYL010000043        20456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2148_length_20456_cov_6.277143, whole genome shotgun sequence.
ACCESSION   DAOSYL010000043 DAOSYL010000000
VERSION     DAOSYL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20456
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_20456_cov_6.277143"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1223)
                     /locus_tag="PLU15_07685"
     CDS             complement(237..1223)
                     /locus_tag="PLU15_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOO30842.1"
                     /translation="MSGCRIIGPEVSNIPWENKPEGYRYVVWRSSRNPIIPRDLLPDS
                     NSIFNSAVVPFGDGFAGVFRCDDRRREMRIHAGFSEDGVEWRIDPQPIEFICDDREIG
                     SLVYAYDPRVCWIDDRYYVTWCNGYHGPTIGVAYTYNFKEFFQLENAFLPYNRNGVLF
                     PRKIGGKYMMLSRPSDRGHTPFGDIFCSQSPDMIHWGCHRHVMSPRGSWQSTKIGPGP
                     VPIETSEGWLLIYHGVLTSCNGFVYHMGAALLDIDRPWKVLCRTEPYILSPQEIYECV
                     GDVPNVVFPCAALADSATGRIAIYYGAADTVTCLAYAYVDELVDFVKSNSTL"
     gene            complement(1264..4041)
                     /locus_tag="PLU15_07690"
     CDS             complement(1264..4041)
                     /locus_tag="PLU15_07690"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOO30843.1"
                     /translation="MRQRMILVAAAVGLLGILCVAVLAAEIPGSLASSRKIVEPKGFK
                     AAAENDSLALFVNGSTTEIAVLDKKTGALWYSNPPDQDERETLARGMARDRLHSQLVI
                     SYFTPGDQRQFMTSCADSVAYGQYHIIPIASGVRVEYVMGAQWQEKDRLPLIISKKRM
                     DEVILAHMESEKDKEFILSSYMLVELRKGGTGERMTLPGWDASTVFRDYTVRLLEDSA
                     SDARWEPSLDEATKWLIAQLVENRSDIEDRSCLKHEDVSQMVDNPTYILMGDTTDWDR
                     EDIASLLAAAGYTADDVVRDHMENHYDPPKPNPRTFSLVIEYVLDGENLIVQVPCSEL
                     EYPIDIVNDEGEKETYPVESISILEFFGAAGQGQDGYMFVPDGSGALVRLNNGKTRFS
                     AVDLTLYGMDQAIMPLDESSAPPQACIPVFGIVANGRAMMAIIESGDAMARIKADVSG
                     RANCYNSVSSSFTVIPKGTVSLSGSGRNISAELKNREVNIYQVRPYQSDICIRYSFLG
                     GSDASYVGMAKCYRDYLRDECGLEKLEPKDDTTFILELVGAARIRKPILGIPTDVVVP
                     LTTCSQAAEVIEALESAGVSEIKMKYTGWFLGGVEHSYPSRVCIDKAVGGASGFAELR
                     DFLGSKGIGLYPDAAFLTVYRSRLFDGFRVGRDGARYLTRRTARIWDYNPATAQRKPG
                     TGRYLLSPARLGSVVSKFNRDYGKLGVGGVSLRDLGRQLYADYREKPDTLVDRGQAKD
                     LITAQVARFAEEYEVMLDGANAYALPYAACAVNLPLDAGGGNIVDESVPFLQIALHGY
                     VEYAGEPINLATDCRHAMLRTIETGACPYYKLMYAPSSALVGTEFNYMYSVGWSDWAD
                     HASEFYRRCNVALHGCGGVEITDHERLAPGVYRTTYSNGRYVIVNYTKEDVIVESCVV
                     GAEDFIAGQAY"
     gene            complement(4079..6250)
                     /locus_tag="PLU15_07695"
     CDS             complement(4079..6250)
                     /locus_tag="PLU15_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOO30844.1"
                     /translation="MIHKRFYTSLAVSVLMLLVYGSASAYAAPYSSYTYDWWKNAVPS
                     ASAYVPSIVVDGADMGVGPLKNPGDLYVRGDRIYVADSGNNRVVCTDRNFRPYMVISS
                     FHVPDGIDGFDEPKGVFVTEAGHIYVADKGNERIVEFDSEGEIVRIIGKPRSDVEGVL
                     SEDMIYRPLRVGVDRAGRVYVIADGIFDGIMEFGVDGGFKGFMGAPKVAPNPWEYFWS
                     KISTKEQKRRRALFLPIEYSSLDIDGRGFIYASVSGGATQKEEAVRRLNPTGVDVLRR
                     TGSFPPVGDVDFPHEESEASITGRSVLIDVAARENGMYSVLDRQRCRIFTYDENGELL
                     YIFGARGDVRGAFRNPVAIDWMGEAFLVLDEANGCITMLEPTEYASFMHQAIDLHNQG
                     KYDEAADMWRNVLRYNANCEAAYIGIGRSLMRQGLYSEAMDNFKLGNSREGYSKAFKL
                     HKKEVANRTFGYVMTAVVVVILALAVLRKVRLFSRSASDGDASELEPMTKYHARMIYR
                     AEEGPITGKRIWDSLKYAFWVICHPFDGFWDLKHERRGNTVSATIILVLIALTHVFAR
                     QYTGFVLNKNDPKRLNVLLEISTVLVPFALWIVVNWSLTTLTDGKGTLKDIYIATAYA
                     LFPIFLINVPLTIWSRYMTMEQGAFFTFFRVLSLIWAGWLVFSGNMVTHDFEGGKTFG
                     MSLLTVAGMGVVIFISLLYASLIDQFIGFVKDIRTEIVFRM"
     gene            complement(6275..7129)
                     /locus_tag="PLU15_07700"
     CDS             complement(6275..7129)
                     /locus_tag="PLU15_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30845.1"
                     /translation="MGSSTGRARRSTGGNVLVCLFLLINGVFMSLPLVYTVSNAFKPL
                     SELFIFPPRLWVRNPTFDNFRDLFVLMARSRMPFSRYLFNTIFISVAGTAGHVIMASL
                     AAYALSKHDFPGRQLMFNVVVLSLMFAPQVTAIPNYLIMSKLGWVDSHAAIIVPAWAS
                     SLGLYLMKQFMDQVPDSLLDAARIDGASEFRVFWQIVLPTVKPAWLTLIILSFQSLWG
                     ETGGRFIYSEQLKTLPYAMSQMVSGGIARAGVGAAVGLLMMIVPITVFLINQSNVVET
                     MSTSGMTG"
     gene            complement(7153..8079)
                     /locus_tag="PLU15_07705"
     CDS             complement(7153..8079)
                     /locus_tag="PLU15_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30846.1"
                     /translation="MTTVATDAELREPHGLGWKIKKHRVSYLFVAPYATFFFLFTVLP
                     VAVAIVLSFTDYNMLESPKWIGWDNYLRLFLVDDVYLIAVKNTFIFAAITGPLSYFLC
                     LIIAWLVNELPPKLRAALTLLFYAPSISGSLFLMWTIIFSGDAYGIINGILLYWGIID
                     QPIQWLTDPKYMMTVVIIVTLWMSLGTSFLVFIAGLQNVDKSLYEAAAVDGIKNRWQE
                     LFFITLPAMKPQLMFGAVMSITSSFAAAGQIIPLVGFPSTDYAAHTVVAHLLDYGNIR
                     FEMGYACAIAVILFVTMVVVQQIVQRILRNLG"
     gene            complement(8101..11124)
                     /locus_tag="PLU15_07710"
     CDS             complement(8101..11124)
                     /locus_tag="PLU15_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO30847.1"
                     /translation="MIPAKLRKTLMAVVALALSMYSATCICVAGGEGSRLVGAESAIP
                     YEEYLSVYSGAEEPQDEIVIDGAAYVSANMPGLETLRDHGGSDGESVLTAGRGYIEWE
                     FTVENPGFYNIEVEYCAAEGRSSALQRELWINGELPFSEAAFLTFSRAWRDTEAVRID
                     NQGNHLRPCQAEAPIWQKAVLSDQMGNYQRPLLFYFGKGNNRIRLVSILEPMIIRSVR
                     LFQMATPKSYAEVSADYGEKGFKAVAGFFEKVQGEDAYLKSSPTLFQVHDAGDPTVEP
                     YHHVLIRLNAIGGDRWTQPGEWIAWHVEVPKDGLYKIALKAKQDYKRGSFTNRRLYVN
                     GDIPFAEVDAIRFPYAINYEMRVLGDDVGEPYLFYLHEGVNEIRLEPVLGDLGPMLLR
                     AEAALYDMNTVYRKIVMITSTNPDPMRDYQLEERVPELISKLAELSEVMRCIGEQLEA
                     YTGARGDYVALLYELARQLGSMAKKPESIPKRIAELRDAGASLGSWILSTREQPLMID
                     YIVVASIEQEMPKAKPTWLQRFKHEVKAFLASFVHRYDLVGNVYSTENGDGVRPLKVW
                     IGAGRDQAQALKSMIEDSFTPATGIPVNLELVSMGVLLPATLSGNGPDVALGLDPAQP
                     LNFALRGAVVSLTQFPDYPEVAARFRPSAMTPFSFRDQVYALPEQQPFPVMFYRKDIL
                     REMGISLPETWDDLIGIIPELQKSHMDIGLPVSGTGAVGAGNVGEAVNAGSVSSNAGV
                     MTFLTLLYQNGGRLYGPDGLCTGLDDEEAVDAFTRWTELHELYTVPLDYNAINRFRIG
                     EMPILITGYTLYNTLSVFAPELRGEWGFTLVPGTRRPDGSIDRSCPAGSCGAGGVPGL
                     ASSVASGPATVIMSSAPDLEGAWEFVKWWTSTETQVRFGREMESLMGPSARYPSANVD
                     AVQELPWKSDECQMLMEQWEWVRGIPEVPGGYMVGRHLDNAFRRVVYHHEPPREVLLD
                     YNRMINEEITMKRLEFGLPVRYDELEAEWCSFDWQ"
     gene            complement(11152..12312)
                     /locus_tag="PLU15_07715"
     CDS             complement(11152..12312)
                     /locus_tag="PLU15_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOO30848.1"
                     /translation="MGGIGAAKSMAKPISIILLVSVLATVVVSASAAGCTGAGGERMY
                     ESSEWRGFHMRFGSHDDVPNLKRVIIEYLIPMGVNVLILEVNTNFQFKSHPELSEGAH
                     TLTHEDAHELAALCRANGIRLIPLFECLGHQGWGGAPNSILRGYPEWDETPHVPVEAE
                     WPTIYCRSWCPLHPEVNPVIFDLMDELIEAFEADAFHVGMDEVFELADDGCPRCAGKK
                     KSDLFAKAVNDYHDHLVTEKGVEMLMWGDRLVDAVGLGYSRWDADVLGIYPAIDMIPK
                     DIIICDWHYDKKAAYPSVYWFFRKGFRVWPATWNDPDAPMAFMEFAYDAREKLDMKDG
                     MLGMLVTGWNATGENLLTALSGGDLGKDEGNIWGIATTLRTVMDEYKRKSAD"
     gene            complement(12346..13503)
                     /locus_tag="PLU15_07720"
     CDS             complement(12346..13503)
                     /locus_tag="PLU15_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOO30849.1"
                     /translation="MDGLQIFSDIGDMQKINRITVLRVLHEHSPLSRLDLAEKSGLSA
                     PTITRIVKELIDEGVVIEAGKAESQRGRTPVLLELNYGGLFFIGIQLNRPKTDVVVSN
                     LKGEVRTYVSDDIDHADPDWVAAKLSEMIETALDDDGIKRKNVLGIGVSVSGLVSSDG
                     KTVLRSDNLCWENVDMRKVLADYSDMPIVVENDAKASLLGELWLGHGAEGSTIVFISA
                     GNGVGASIYHDGVILRGAFGAAGEIGHSPIVYRGKQCWCGNQGCLEAYVGLRQVQEEY
                     KMLTSSSKRVLEAYHDGDPVAASLVRRAAEMLGWSVLHCAYYVNPDQVIIGGKWLEAG
                     DEFLDVLRQTVEEGFALDFSMRPKISYSSLYPHTEVLAAVGLIINEFFQYK"
     gene            complement(13515..16652)
                     /locus_tag="PLU15_07725"
     CDS             complement(13515..16652)
                     /locus_tag="PLU15_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOO30850.1"
                     /translation="MYFLLEHVARLAQELKEHVVSAKAPIEGWSIKEGDFSRPDDVDQ
                     ASAEWSKFLPDMRWGGRDSHAWFRADAAISESFAGKPVALNVHTGDKGWDATNPQFLL
                     YVDGKIVQGLDVNHCEAILCSSGEPGRIYRIDLDAYSGMRESRPSLYAELIAIDEKVR
                     KLYYDIVVPLGVVQNLPEDDKTRIDMLLVLNEALNRVDFRSPRSASFHCSIDRAQAYL
                     EEELYGKMCGHGDVLAACVGHTHIDVAWLWRIAQTREKAARSFSTVLKLMDEYPDYVF
                     MSSQPQLYKFVKEDYPALYERIGERVREGRWEPEGAMWVEADCNVVSGESLVRQIMFG
                     KRFFAEEFGVDNKILWLPDVFGYSAALPQIMKKSGIDYFMTTKISWNQYNKLPYDTFM
                     WRGIDGSEVLTHFITTRDYETSPTSWFATYNGDLKPSQVMGAWQRYQQKALNNDVLIS
                     YGHGDGGGGTTREMIENGLRMARGIPGCPRVRMATVRSFFDRLEKTVSGNRRLPTWVG
                     ELYFELHRGTYTSMGRNKRANRKSELLYGDVETLSTFAMLMGADYPQDEINRNWERIL
                     TNQFHDILPGSSIKEVYDDSQEDYDCIISTGHELARNALDAIVSSIGTDDVSVVVFNT
                     LGYDRDEIAEFALPENVEHPCLVDSDGGIIPCQVQAVNGKRIGLFIAHGVPSKGYKTY
                     RLLDRPTQVPSGLAVSSFKLENAYFELQLDEKGTFISIYDKVNQRQVLKKDERGNRLL
                     AFQDMPANWQNWDIDISYQEKVWEVDRVDKMEIVEEGPVRAGIRIHKTFFDSKIVQDV
                     LIYSDIPRIDFKTYIDWKQSQILMKAAFPVDVNAEKAAYEIQFGNVERPTHWNTSWDV
                     ARFEVCGHRWADLSEGGYGVSLMNDCKYGYDIKDGEIRLTLLKSGIEPNPVADQEEHW
                     FTYSIYPHAGDWKTASTTRAAYSLNVPLRAEVVGPQPDGSLPKSLSMLRIDADNVMLE
                     TVKKAEKSDEIVVRLYEFQNKRTNVNVAFFGELEAVSECDLLENDIDSVVAQGSTFSF
                     EIKPYEIKTFKLKVK"
     gene            complement(16831..18120)
                     /locus_tag="PLU15_07730"
     CDS             complement(16831..18120)
                     /locus_tag="PLU15_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO30851.1"
                     /translation="MTRIIACAVLVVGLLAGLPGPGAAASYDLKGKTVTFVGWWDYLD
                     KFAPGKEREGRIQEAEKKFNCKIVAKPLNWDDYTSLLMTRLLSGDSEYDVWLMNHNFF
                     WQLAARDAFCPLDKVLPQSYYKSLPKARQGAVKVMSHGGNTYGFGSYGNILGCLRFMA
                     YNKTMFEREGFPDAQKLYEQGKWDWDHCLDIANKATKDLDGDGNPDQWGLSSLWHWGV
                     ALSNDGKLTRADSKGRPIFTGDEINVTEGMAMYLDLYEKYKVCLDDDWGGDQFVAGKV
                     AMQPLEFWRFEFIGPKMKDRWGIVPVPKGPRAADYRYPNLQLESLVLPANSKDPKAFV
                     ALADFLFTAEDHDNDISGSIKAGCPDRVSAEIVKRGVENWKGEMYDLNLGAVAGQVWS
                     DALMAWLNGEKSAAVAMKEVKPAIQTAINDFFAPKKK"
     gene            complement(18288..19259)
                     /locus_tag="PLU15_07735"
     CDS             complement(18288..19259)
                     /locus_tag="PLU15_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30852.1"
                     /translation="MEATKEAVRGVAPPGQREVRQQHAKRPPFWRKAGRIVWRILRAG
                     LIIGICYVILMPLMTKISSSFMEVRDMFDVTVRWIPRHFTLENYKVVWEAMKYPTAFW
                     NSLKLSLIVAVLQTFSSALVGYGFARFKFKGSNLLFALVLVTLLVPSQTILVPLYLNF
                     RYFNLFGLIPGQGLNLLNSYWPFILIAMTGAGMRGGLFIYVMRQYFRNVPKELEDAAY
                     VDGASPLRTFFTVMLPGAVPSMVTIFLLSFVFQWNDSTLTSVFMGNEVLLPWTLRTLA
                     DTLGARAEYASLVNNTGMLLFMAPLLILYFVMQKHFVEGVERTGIVG"
     gene            complement(19316..20221)
                     /locus_tag="PLU15_07740"
     CDS             complement(19316..20221)
                     /locus_tag="PLU15_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30853.1"
                     /translation="MKHSTRRLTLERRRAVSGYLFTLPFTIGFLVLFLVPFIQSIAFS
                     LSELRITSTGYQLDYLGLKNFHDAILVQPDFNKKLIEELGKMLRQVPLVIVFSFFAAV
                     MLNEEFPGRSVARTIFFLPVIMSSGVVFFMEKTDYILQTVAAGTQAAVAEGLVGGSAI
                     RAFLWQLKLPEWFMSYIVSAVDNIPVVIRSSGIQILIFLAGLQSIPGSLYEAADVEGC
                     TRWERFWLITFPMLSPLVMTNLVYTIIDFLTGRQSEFLSWIQDFTFRASYGMGTAMAL
                     LYFLAVGIIVAIVVSIVSKWVFYQE"
BASE COUNT         4931 a         5972 c         4656 g         4897 t
ORIGIN      
        1 cattatcatc atcacatgac cttcttgaac ttccacaaca gcatgcttca gaattcatat
       61 tggaccattc tgcacgaact acgtgccctc atccggagaa tccaggccca aacggcttgc
      121 aacccctcga gccgccttcc agccgtttcc tagcctgtga gtcaaaagcc gacaggcagg
      181 ggttgaagtt cagcctccct acgattggcc aaagccgaga gggccacaca ggctagctat
      241 agtgttgagt tgctcttcac gaaatccacc aattcatcta cataggcata tgccaagcat
      301 gtcactgtat cggcagcgcc gtagtagatt gctatacgcc cagtggcaga atctgcaaga
      361 gctgcacacg gaaaaacaac attcggcacg tcccctacgc actcatatat ctcctgtgga
      421 gatagtatat aaggttctgt cctgcagaga accttccaag gtcgatctat gtcgaggagg
      481 gctgcgccca tgtggtacac aaacccattg cacgatgtga ggactccatg atagatgagg
      541 agccatccct cactggtctc aataggcaca ggtccagggc cgatcttcgt gctctgccac
      601 gatcccctag gcgacatcac gtggcggtga caaccccagt gaatcatatc agggctctgg
      661 ctgcagaata tgtcgccgaa cggcgtatga cctcgatcac tcggtcggct caacatcatg
      721 tacttgccgc ctatcttgcg cggaaatagc actccattcc tgttgtacgg cagaaatgcg
      781 ttctcaagct ggaaaaactc cttgaagttg tacgtgtatg ccacgcctat cgtcggccca
      841 tggtatccat tgcaccatgt gacataatac cgatcatcta tccagcagac acgggggtca
      901 tacgcgtaga caagactccc gatttcgcgg tcatcacaga tgaactctat gggctgcgga
      961 tcaatgcgcc attcgactcc gtcctcacta aagcctgcat gaatgcgcat ctctcgcctg
     1021 cgatcatcac agcgaaagac tccggcaaat ccatctccga agggcacaac cgcgctgttg
     1081 aatatggagt tcgaatcggg caacagatcc ctgggaatta tcggatttcg ggatgatctc
     1141 catacgacat atctgtatcc ttcgggcttg ttctcccagg gtatattgga aacttcaggc
     1201 ccgattatcc tgcatccgct catgacgact cctcctgtat ccgctctttc tccaacagcg
     1261 tcttcagtac gcctgtccag ctatgaagtc ctccgcacct accacgcagg actccacaat
     1321 aacgtcttcc ttggtgtagt tgacaatgac atatctgcca tttgagtacg tggtccggta
     1381 taccccggga gccaaccttt cgtggtccgt tatttccaca ccaccacagc catgcagcgc
     1441 cacattgcat ctccggtaaa actcggacgc gtggtcagcc cagtcgctcc accctacaga
     1501 atacatgtag ttgaactctg tgcctacaag cgcagatgat ggcgcgtaca tcagcttata
     1561 gtaggggcat gcccctgtct caatagtccg tagcatagca tgcctgcagt ctgtagcaag
     1621 attaatgggc tcaccagcgt attcaacgta gccgtgcagc gcaatctgga ggaacggaac
     1681 actctcatcc acaatattcc cgccccctgc gtcgagagga aggttcactg cacatgcagc
     1741 atatggcaga gcatatgcat tagccccatc gagcatcact tcgtattctt cagcgaatcg
     1801 cgccacttgt gctgttatca gatcctttgc ctgcccgcga tcgacaaggg tgtcaggctt
     1861 ctctcgataa tcggcataga gctgtcttcc taagtcgcga agggatactc ctcccacacc
     1921 aagcttcccg tagtcgcggt taaacttcga tacaacactc ccaagtcttg cgggggacag
     1981 aagatatctg cctgttccgg gcttgcgctg agcagtggcc ggattgtaat cccagatccg
     2041 cgctgtgcgc ctagtgaggt atcgagcccc atcgcgcccg actcggaaac cgtcgaacag
     2101 cctgcttcgg tataccgtca gaaatgctgc gtcaggatag aggcctatgc ctttcgaacc
     2161 caggaaatca cggagctctg caaaaccaga tgctcccccc actgccttgt caatgcagac
     2221 ccttgacggg tacgaatgct ccacaccgcc aagaaaccag cctgtatact tcatcttgat
     2281 ctcagaaacc ccggcgctct ccaatgcctc gataacctct gccgcctgag agcaggtggt
     2341 caaaggtact actacatccg taggtattcc gagtatgggt ttgcggatgc gggctgcgcc
     2401 taccagctca agaatgaacg ttgtgtcatc tttcggttcc aacttctcaa ggccacactc
     2461 gtcacgcaga tagtcgcggt agcacttagc catgcctaca tagcttgcat cgctgccacc
     2521 gagaaatgag tagcggatgc agatatcgct ttgatagggt cgaacctggt atatgttgac
     2581 ctccctgttc ttgagttccg ccgagatatt cctcccgctg cccgacaatg agacagttcc
     2641 cttcggaatc accgtgaaac tggacgagac actattgtag caattggcgc ggcccgacac
     2701 atccgccttg attctggcca tggcgtctcc actctctatg atggccatca tagcccgccc
     2761 gttcgctacg atgccgaata ctggaatgca cgcctgaggc ggcgcactgc tctcatcaag
     2821 tggcattatg gcctgatcca tcccgtaaag ggtgaggtcg actgctgaaa aacgcgtctt
     2881 cccgttattc aaccgaacta gcgcccctga accgtcgggg acgaacatgt agccgtcttg
     2941 gccttgtcct gcggcgccga agaactcaag tatacttatg gattctactg ggtatgtctc
     3001 cttttcaccc tcgtcattga cgatgtctat ggggtattcc agttcagagc agggcacttg
     3061 cacgatcaga ttctcaccgt caagcacata ctcaatcacc aacgagaagg tcctcggatt
     3121 cggcttcggg ggatcatagt ggttttccat atgatcgcgc accacatcgt cggcggtgta
     3181 gcctgcagcc gcaagtaggc ttgctatgtc ttccctatcc caatcagttg tgtcgcccat
     3241 cagaatgtaa gtcggattgt ccaccatctg agatacatcc tcgtgcttta agcacgatct
     3301 gtcctctatg tccgacctgt tctcgacgag ctgcgcaatg agccacttgg tggcttcatc
     3361 gagagacggt tcccatctgg cgtccgacgc agagtcctca agcaatctta cagtatagtc
     3421 gcgaaagact gtggaggcat cccatcctgg caaagtcatg cgctcaccgg tgccgccctt
     3481 cctgagctct accagcatgt agctggataa gatgaactcc ttgtcctttt cgctctccat
     3541 atgcgccaaa atgacttcat ccatccgttt cttgctgatg atcagaggga gtctgtcctt
     3601 ttcctgccac tgcgcaccca ttacgtattc aacccgcaca ccactagcaa taggtataat
     3661 gtggtactgc ccgtaagcaa ctgaatcggc gcaactcgtc atgaattgac gttgatctcc
     3721 tggagtaaaa tacgagataa ccagctgtga atgtagccta tccctggcca ttcccctggc
     3781 caaggtctcg cgttcatcct gatccggcgg attcgaatac caaagggcgc ccgtcttctt
     3841 gtccagcaca gcgatctcag ttgtgctccc attgacaaac agcgcaagtg agtcattctc
     3901 tgcagctgct ttaaagcctt tgggttctac gatttttctg gaacttgcta ggctccctgg
     3961 tatctccgca gccaggaccg ccacgcataa tatgccaagc agcccgactg cggctgccac
     4021 aaggatcatt cgctgcctca tgtattcaag acctccgttc gccccaatat ccaaacgcct
     4081 acattctgaa gactatctca gtgcgaatat ccttcacaaa accgatgaac tgatctatca
     4141 ggctggcata gagcagggaa atgaaaataa ccactcccat gccagctacg gtcaagagcg
     4201 acatcccaaa cgtctttccg ccttcaaaat cgtgtgtaac catgttgcct gaaaagacta
     4261 gccaacccgc ccatattaac gacagcaccc tgaagaacgt gaaaaacgcg ccctgttcca
     4321 tcgtcatgta cctgctccag atggttaatg gaacgtttat caggaatatc ggaaagagcg
     4381 cgtaggccgt tgctatgtat atgtctttca gcgtcccctt tccgtctgtc agtgttgtga
     4441 gcgaccagtt tacaacaatc caaagggcga acggcaccag cacagtgctg atctcgagca
     4501 gcacgttcag cctcttggga tcatttttgt taaggacgaa gcctgtatac tgccttgcaa
     4561 acacatgtgt caaagcaatg agaacaagaa tgatggtggc agacacggta ttcccgcgac
     4621 gctcgtgttt caaatcccag aagccatcga atggatggca tattacccag aaagcgtact
     4681 tcagcgaatc ccatatccgt tttcctgtga taggcccctc ttccgcgcga tagatcatcc
     4741 tggcatggta tttggtcatc ggctcaagct ctgacgcatc gccatcggat gcactgcgcg
     4801 aaaacaagcg cacctttctg agaacagcaa gtgccaatat taccaccact acagccgtca
     4861 tcacataacc gaaagtgcgg ttggccactt ccttcttgtg aagtttgaag gccttggaat
     4921 agccttccct gctgttcccc aatttgaagt tgtccatagc ttcactgtat agaccttgac
     4981 gcatgagcga tctgcctatg cctatatagg ccgcttcaca gttcgcgttg tatctgagaa
     5041 cgtttcgcca catatcggca gcttcatcgt acttcccttg gttgtgcagg tcgatagctt
     5101 gatgcataaa tgaagcatat tctgtgggtt caagcattgt gatgcacccg tttgcctcat
     5161 ctaggacgag aaacgcctct cccatccagt caatggccac agggtttctg aaagcgcctc
     5221 tgacatcccc tcgagcccca aaaatataca gaagctcgcc gttttcatcg tatgtaaaga
     5281 tgcggcatcg ctgcctgtcg aggactgaat acatcccatt ttcacgcgcg gcaacatcga
     5341 tcaacacaga acgcccagtg atgcttgctt ccgattcctc atgtggaaaa tcaacatcgc
     5401 ctaccggcgg aaaagaaccc gtccttcgca gcacatccac gcctgtgggg ttgagacgcc
     5461 gaaccgcctc ttccttctgc gtagcgcctc ccgagactga ggcgtatatg aaaccacgcc
     5521 cgtcaatgtc gagactactg tattctattg gcaggaacaa tgctcgtctt ctcttctgct
     5581 ccttggtgga aatcttcgac cagaagtatt cccagggatt cggggcaacc ttcggagcac
     5641 ccatgaaccc cttgaagccc ccatctacgc cgaactccat tatcccatcg aaaatgccat
     5701 cggctatgac gtagacgcgt cccgccctgt ccacgcccac tcttaagggt ctatagatca
     5761 tgtcttctga caagacaccc tctacatcag accgaggctt gccaatgatc ctcacgattt
     5821 cgccttcaga gtcgaactcg actattcttt cgtttccctt atctgccacg taaatgtgtc
     5881 ctgcctccgt cacgaaaacg ccctttggtt cgtcgaagcc atcgatgcca tccgggacgt
     5941 ggaatgatga tatcaccatg tacgggcgga agttccggtc cgtgcagacg actctgttgt
     6001 tgccggaatc agcaacgtag atccggtcgc ccctcacata aaggtcaccc gggttcttca
     6061 agggcccgac ccccatatcc gccccgtcta ccacgattga gggaacatag gcggatgcgg
     6121 acggaacagc gtttttccac caatcatacg tatagctact gtagggcgcc gcataggcag
     6181 aagcactccc atacaccaac agcattagca ccgatacagc caatgatgtg tagaatctct
     6241 tgtggatcat cagtacgctt ctcctttcag cgggttagcc cgtcattccc gacgtgctca
     6301 tggtctctac cacgttgctc tggtttatca ggaacacagt tattggcact atcatcatca
     6361 gcaaacccac cgcagcccca accccggctc tggcgattcc gcccgaaacc atctggctca
     6421 tggcatatgg caaagtctta agctgttccg agtaaatgaa ccgccctcct gtctcacccc
     6481 acagcgattg aaaggagagt atgatcagcg taagccatgc aggcttcact gtaggcaaaa
     6541 cgatttgcca gaataccctg aattcgcttg ctccatctat cctcgcagca tccaataagg
     6601 agtctggaac ctgatccatg aattgcttca tcaagtatag tcctaacgat gacgcccatg
     6661 ccgggactat gatcgcagcg tgagagtcaa cccaccccag cttcgacatg atcaagtagt
     6721 tgggaatcgc cgtaacttgc ggagcaaaca tcagcgaaag cactactaca ttgaacatga
     6781 gttgccttcc ggggaaatca tgtttggata gcgcataggc ggcgagggac gccatgatca
     6841 catgcccggc ggttcccgca acgcttatga agatcgtgtt aaacaaatac cgggaaaatg
     6901 gcatcctcga acgcgccatc agtacaaata gatcccgaaa attgtcgaaa gtcggattcc
     6961 gcacccagag ccgtggcggg aatatgaaga gctcgctaag gggcttgaat gcgttgctga
     7021 ccgtatagac caatggcaac gacatgaaga cgccgttgat taagagaaat aggcatacca
     7081 gcacgttccc tccggttgag cgtctcgctc gtccagtact acttcccata acaaatgccc
     7141 tccccgactg ccctatccaa ggttcctcag aatcctctgg acaatctgct gcaccacaac
     7201 catagttaca aagagtatca ctgcgattgc acaagcatat cccatttcaa atcgaatatt
     7261 cccgtagtcg agcagatgcg ctactactgt gtgggccgca tagtcggtgc ttggaaaacc
     7321 gaccaaagga attatctgcc ctgccgctgc gaacgaggaa gtgatggaca tgactgcacc
     7381 gaacatgagc tggggtttca ttgccggaag agttatgaag aacagttcct gccacctatt
     7441 cttgatcccg tctacggcag cagcttcata tagactctta tctacgttct gaagaccggc
     7501 gataaacacc aaaaagcttg ttccaagact catccataag gtgacgataa tcactacagt
     7561 catcatgtac ttcggatcgg tcagccattg aatcggttgg tcaatgattc cccaatagag
     7621 cagaatgccg tttattatgc cgtatgcatc accactgaag atgatcgtcc acatcaagaa
     7681 aaggctgcct gatatggacg gggcgtaaaa cagcagcgtc aaggcggccc tcagttttgg
     7741 cggcaactca ttgaccaacc acgctatgat caaacacaga aaataactga gcggacccgt
     7801 tatcgccgca aagatgaagg tgttcttcac agctattaga tacacatcgt ccaccaagaa
     7861 caggcgaaga taattgtccc accctatcca cttgggcgac tccaacatgt tgtaatctgt
     7921 gaaactcaac actatcgcta ccgccaccgg caagactgtg aacaggaaga agaatgtagc
     7981 gtaaggagca acgaacaggt aagacaccct gtgcttcttt atcttccatc ccagtccatg
     8041 aggttctctc agctcagcat ctgtcgctac tgtggtcacc gtcctacccc tccttcacca
     8101 tcactgccaa tcgaaactgc accactctgc ctcgagctcg tcgtagcgga ccggtaaccc
     8161 gaattccaac cgtttcatag ttatctcttc attaatcatc ctattgtaat cgagcagaac
     8221 ctcacgaggg ggttcatgat gatacacaac tcgtctgaat gcgttatcta ggtgtctgcc
     8281 caccatgtag ccgccgggaa cctccggtat gcctcgtacc cactcccact gctccataag
     8341 catctggcac tcatcggatt tccaaggaag ctcctggaca gcatcgacat ttgccgaagg
     8401 ataacgtgct gacggcccca tcaagctctc catctcacgt ccgaacctga cctgggtttc
     8461 ggtgctagtc caccatttta cgaactccca cgccccttct aggtcaggcg cagagctcat
     8521 tatgactgtt gctggcccgg aggccacaga cgaagctaac cccggcacgc ccccggctcc
     8581 acaactaccc gcaggacacg atctgtctat gcttccatcc gggcgtctcg tgcccgggac
     8641 cagtgtaaac ccccactctc ctctgagctc cggcgcaaag accgacagcg tgttgtagag
     8701 tgtgtagccc gtgataagta tcggcatttc gccaattctg aatctgttga tggcgttgta
     8761 gtcaagggga acagtataca gctcgtgcag ctctgtccac ctagtaaagg cgtcaacagc
     8821 ttcctcatcg tcaaggcctg tacataatcc gtcgggaccg tacagccgtc cgccgttctg
     8881 ataaagcaag gtaaggaaag tcattacccc agcatttgat gagacgcttc ccgcattcac
     8941 tgcctcgcca acgtttcccg ctcctactgc tccagtgcct gaaactggca gaccaatgtc
     9001 catgtgcgat ttctgcagct caggaatgat accaatcaaa tcgtcccagg tctcagggag
     9061 cgaaatgccc atctccctca gaatatcctt tctgtagaac atcacaggga acggctgctg
     9121 ttcgggcaag gcatacacct gatcccggaa agagaaaggc gtcatcgcac tgggcctgaa
     9181 ccttgccgca acttcaggat agtcaggaaa ctgagtcagg ctgacaacag ctcccctcag
     9241 ggcgaaattg aggggctgtg ctggatcgag tcctagggca acatctggac cgttccctga
     9301 aagagttgcc ggcagcaaca cccccatcga gacaagttcg agattgaccg ggatccctgt
     9361 ggcaggagtg aaagaatctt ctatcatgga tttcagtgct tgagcctgat cgcgtcctgc
     9421 acctatccat actttgagcg gtctgacgcc gtccccattt tctgttgaat atacattacc
     9481 taccaaatcg tatctgtgta cgaatgatgc aaggaaggcc ttaacctcgt gtttgaacct
     9541 ttggagccaa gtcggttttg cctttggcat ctcttgttca atcgatgcaa cgacgatgta
     9601 gtcgatcatg agtggctgct cccgagtgga aagaatccac gatccaagag aggccccggc
     9661 atcacgcagt tctgcgatgc gctttggaat gctctcaggc tttttcgcca tactgcccaa
     9721 ctgccgcgca agctcataca aaagggccac gtagtccccg cgagcacctg tatatgcctc
     9781 aagctgctct cctatgcagc gcatcacctc gctcagttca gccaatttcg agatgagttc
     9841 gggcactctt tcctccagct ggtaatcgcg cataggatcc ggattcgttg atgttatcat
     9901 cacgatcttc cggtaaactg tgttcatgtc gtaaagggct gcctctgccc gaagcagcat
     9961 gggcccgaga tcgcccagta ccggctcaag ccttatctca ttgacccctt catgaagata
    10021 aaagagatac ggctccccaa catcgtcccc aagcactctc atctcgtagt tgatcgcata
    10081 tggaaacctt attgcgtcca cctcggcaaa cgggatatcg ccatttacgt agaggcgacg
    10141 gttggtgaac gaccctcgct tgtaatcctg cttcgccttg agggcgatct tgtaaaggcc
    10201 gtccttaggc acctccacgt gccacgcaat ccattctcca ggctgggtcc atctgtctcc
    10261 gccaatcgca ttgagcctta taagaacatg gtggtatggt tctacagtcg gatctcctgc
    10321 atcatgcact tgaaacagag ttggactgga tttgagatat gcgtcctctc cttgcacctt
    10381 ctcaaaaaag cccgccacgg ctttgaagcc tttttctcca taatccgcgc tgacttcagc
    10441 atagcttttg ggggtagcca tctgaaaaag acgcaccgac cggataatca ttggctccag
    10501 aatggatacc agtcttatcc tgttattgcc cttgccaaag tagaacaaaa gcggcctctg
    10561 ataattcccc atctggtcag aaagaacagc cttctgccat attggagcct cagcctgaca
    10621 gggtctgagg tggttcccct ggttatctat gcgaacagct tctgtgtccc gccatgccct
    10681 actaaaagtg agaaatgcag cttcggagaa tggcagctca ccgttgatcc atagttctcg
    10741 ctggagcgcc gaacttctgc cctccgcagc acaatactct acttcgatat tgtagaagcc
    10801 agggttttcc acggtaaact cccactcgat gtagccccga ccggcagtaa ggaccgactc
    10861 accgtcactg ccaccgtgat ctctcagcgt ctccaagcca ggcatattcg ctgacacata
    10921 ggcagcccca tcaatcacga tctcatcctg aggttcttcg gcgccgctgt aaacactcaa
    10981 gtactcctcg tatggaatgg cagactctgc acccacaagc cttgagcctt cacctccagc
    11041 aacgcaaatg caagtcgccg aatacatgct caaggctaac gccaccactg ccatcaacgt
    11101 ctttcgcaat ttggcaggaa tcataagccc aactcccagc gcttatatgt gttagtcagc
    11161 tgacttccgc ttgtactcgt ccattacagt cctgagtgtg gtcgcaatcc cccagatgtt
    11221 tccctcatcc ttgcccaagt cgccgccgct caatgcagtc agaagattct cgcccgtagc
    11281 attccaccct gtcacaagca tgccaagcat cccgtctttc atatccagtt tttcccgtgc
    11341 atcatacgca aactccatga aagccatggg cgcatccggg tcgttccatg tcgcaggcca
    11401 cactctgaat cccttgcgga agaaccagta taccgaaggg tacgcagctt ttttgtcata
    11461 atgccaatcg catatgatga tgtccttggg aatcatgtcg attgcaggat atatgcccag
    11521 aacatcagcg tcccacctgc tgtatcccaa ccccaccgcg tccacaaggc gatcgcccca
    11581 catgagcatt tctacgccct tctctgtgac aaggtggtca tggtaatcgt tgacagcctt
    11641 ggcaaacaga tctgacttct tctttccggc acaccttgga catccatcgt ctgccaactc
    11701 aaaaacctca tccattccga catggaaagc atcggcctca aaggcctcga tcagctcgtc
    11761 catgagatcg aagatgaccg gattgacctc tggatgcagc ggacaccagc tgcggcaata
    11821 gatcgtcggc cactcggcct ccaccggcac gtgaggcgtc tcatcccatt caggataccc
    11881 cctgagtata ctgttcggcg cgccccccca tccctggtgc ccaaggcatt caaagagcgg
    11941 tatcagtctt atcccattcg cgcggcagag cgccgccagt tcatgagcgt cctcgtgtgt
    12001 gagggtgtgc gccccctctg aaagctcagg atgggatttg aactggaaat tcgtgtttac
    12061 ctctaggatc agcacgttca ctcccatagg aatgaggtat tctatgatga ctctcttgag
    12121 attggggaca tcatcatgag aaccgaacct catgtgaaat ccacgccatt cactagattc
    12181 gtacattctt tcacccccgg ctccagtgca accagctgct gacgcagaga caacgaccgt
    12241 cgccagcaca ctcactagca ggattatcga aataggcttt gccatgctct tggcggcccc
    12301 aatgcccccc atgctcaatg ccctcctatc aagcgctctc aacactcact tgtattgaaa
    12361 gaattcattg ataatgagcc ccaccgccgc aagaacctct gtgtgtgggt ataggctgga
    12421 gtaggagatc ttcggcctca tagaaaaatc cagtgcaaac ccttcctcca ctgtctgccg
    12481 gagaacgtcg aggaattcgt ccccggcttc aagccacttc ccaccaatta tgacttgatc
    12541 agggttgaca tagtatgcgc aatgaagaac actccatcca agcatctccg ctgccctacg
    12601 cactaggctt gcggcaaccg gatctccgtc gtgatatgcc tccagcactc tcttcgatga
    12661 agaagtgagc atcttatact cttcctggac ttggcgaagt ccgacatagg cctcgaggca
    12721 accttgattt ccgcaccaac actgcttgcc cctgtacaca atgggagaat gccctatctc
    12781 cccggccgcc ccaaaggcgc ctctaagaat cacaccgtca tggtatatac ttgcacctac
    12841 accgtttcca gcgctgatga agacgatagt cgaaccctca gctccatgcc ctagccacaa
    12901 ctctccgaga agagaggctt tagcatcgtt ctctaccaca atcggcatat cactgtagtc
    12961 agcaagcacc tttctcatat ctacattttc ccagcataag ttgtcggatc tgagcactgt
    13021 cttcccatca gatgacacaa gccctgatac tgatactccg attcctaaca cgttctttcg
    13081 tttgatgccg tcatcatcca gagcggtctc aatcatctcc gacagctttg cagctaccca
    13141 gtccgggtcc gcatgatcta tgtcgtctga aacataagtc ctaacctctc ccttgaggtt
    13201 actcacaact acgtcggtct taggcctatt gagctggatg ccgatgaaaa acagtccccc
    13261 gtagttgagc tcgagcaaga cgggagtgcg cccacgctgt gactccgctt tgcccgcctc
    13321 aatcacgacc ccttcatcta tcagctcttt cactatcctg gtaatggtcg gtgcagaaag
    13381 cccggatttc tcagccaaat cgagcctgga caggggggag tgctcgtgga gcaccctcag
    13441 aaccgtgatt ctattgatct tttgcatgtc gcctatgtca ctgaaaatct gaagtccgtc
    13501 cacttggcat cactctactt cactttgagc ttgaacgtct ttatctcgta aggctttatc
    13561 tcgaatgaaa acgtgcttcc ctgtgccacg acagagtcga tatcgttctc aagcagatcg
    13621 cattctgaca cagcctcaag ttctccgaag aaggccacgt tcacattggt ccgtttgttc
    13681 tggaattcgt atagtctcac aacgatctca tcggactttt ccgccttctt caccgtctca
    13741 agcattacgt tgtcagcgtc tattctgagc atggacaggc ttttcggaag gcttccatcc
    13801 ggctgtggcc ccaccacctc ggcgcgcaac ggcacgttga gtgagtaggc agccctggtc
    13861 gtagatgccg tcttccagtc gcctgcatgc ggatagatcg aatacgtaaa ccaatgctct
    13921 tcctgatcgg ctacgggatt gggctcgatt cccgatttca gcaaggtcag acggatctct
    13981 ccatccttta tgtcataacc atatttgcag tcattcatga ggcttacacc gtagccaccc
    14041 tcagaaaggt ctgcccacct gtgcccgcaa acctcgaacc gtgcaacatc ccatgaagtg
    14101 ttccaatgcg tcggcctttc cacattgccg aactgaatct cgtaagccgc cttctcagca
    14161 tttacgtcca ccggaaaggc tgccttcatg aggatctggg attgtttcca gtcgatgtaa
    14221 gtcttgaagt ctatccgcgg gatgtcggag tatataagaa catcttgaac gatcttcgaa
    14281 tcaaaaaatg tcttgtggat cctgatcccc gcacgtacgg gaccttcctc cacaatctcc
    14341 atcttgtcaa ctcggtcaac ttcccacacc ttctcctggt atgagatgtc gatatcccaa
    14401 ttctgccagt tagcaggcat gtcctgaaat gccaggagcc tgttgcctcg ctcgtccttt
    14461 ttcagaacct gcctctgatt taccttgtca tagatggata tgaaggttcc tttctcgtcc
    14521 agctgaagct cgaaataggc gttctccagt ttgaaactgc tcactgctaa accactagga
    14581 acctgtgttg gcctgtccaa gagtctgtat gtcttgtatc ctttggaggg cactccatgt
    14641 gcgatgaaaa ggccgattct ctttccgttc acagcctgca cctgacaagg gataatcccc
    14701 ccgtcgctat caactaggca gggatgctct acattctcag gaagagcgaa ctctgcaatt
    14761 tcgtcacgat catatcccaa tgtgttgaaa accacgactg atacgtcatc agtgcctata
    14821 cttgaaacaa ttgcatccag tgcattccgg gccagctcat ggcctgttga gataatgcaa
    14881 tcgtaatcct cctgcgaatc atcatacact tccttaatgg acgatcctgg caatatgtcg
    14941 tggaactggt ttgtaaggat tctctcccag tttcgattga tctcatcttg cggataatct
    15001 gcacccatca acatagcaaa ggtcgacagc gtctctacgt ctccatacag cagttcgctc
    15061 ttgcgatttg ctcgcttgtt tctccccatt gaggtatatg tccctctatg cagttcgaag
    15121 tagagttcgc caacccacgt aggcagccga cggtttcccg atacagtctt ctcgagtcgg
    15181 tcaaagaaac tgcgtacagt cgccattctc acccttgggc agcctggtat tccccgcgcc
    15241 atcctaagtc cgttctcaat catctctcga gtggtgcccc caccgccatc accgtgaccg
    15301 taagagatca gaacatcatt gttcaaagcc ttctgctgat agcgctgcca tgcccccatg
    15361 acttgagacg gtttcagatc gccattgtat gtggcaaacc aagaagtggg cgacgtctcg
    15421 taatcccttg tcgtgatgaa atgcgtcaac acttccgatc cgtcgattcc cctccacatg
    15481 aacgtgtcgt aagggagctt gttgtactgg ttccagctga tcttcgtggt catgaaataa
    15541 tcgatgccgg atttcttcat tatctgtggc agggctgcac tgtaaccgaa tacatcagga
    15601 agccatagga tcttgttgtc gacaccgaac tcctcagcaa agaaccgttt gccgaacatt
    15661 atctgtcgca ccaaagactc gccagacacg acattgcagt cggcctcaac ccacatagcg
    15721 ccttccggct cccacctgcc ctcacggact cgttccccga ttctctcata cagagccggg
    15781 tagtcctctt tcacgaactt gtagagctga ggctggctag acatgaaaac gtaatcggga
    15841 tactcgtcca tcagcttgag cactgttgaa aaactcctgg ccgccttctc gcgcgtctgc
    15901 gcgatcctcc atagccaggc cacatctatg tgcgtatgtc ctacgcacgc ggctagcacg
    15961 tccccgtgcc cacacatctt cccatacagt tcttcctcga ggtaggcttg ggccctatca
    16021 atagaacaat ggaacgatgc ggagcgaggg cttcgaaaat ccacgcggtt gagtgcctca
    16081 ttgagcacga gcaacatgtc gatccgggtc ttgtcatctt cgggaagatt ctgcactacg
    16141 ccgaggggca cgacgatgtc gtagtaaagc ttacgcactt tctcatcaat ggcgatcagt
    16201 tcagcataga gcgacggccg cgattcacgc attccgctgt atgcatcgag gtcaatgcgg
    16261 tagatacgcc ccggttcacc gctggagcag agaattgcct cacagtgatt gacatccagc
    16321 ccctgaacga tctttccgtc aacatacaga aggaactggg ggttggttgc gtcccatccc
    16381 ttatctcccg tatgaacgtt gagggcaaca ggcttgccgg caaaagactc tgaaatggcg
    16441 gcgtcagccc gaaaccatgc atgactatct cttccacccc accgcatgtc gggtaggaac
    16501 ttgctccatt cagctgatgc ttggtccaca tcgtcaggcc ttgaaaagtc gccttccttg
    16561 atactccatc cttctattgg agctttcgct gaaacaacat gttctttcag ctcctgagca
    16621 agtctggcta catgttcaag aaggaaatac attgctgtgc cccctaacat cgcatgcctg
    16681 atgattcact ccaaaggcgc ccacccgacc gtacctgcag ctgattctgc cgaagggagc
    16741 tagcccctct tggtgcgtcg cggttgatcc tttgagattg agttctgctc ggacccgaat
    16801 ccgagcagaa ctcttcgata tacacaaaga ctacttcttc ttaggagcaa agaagtcatt
    16861 tatcgccgtc tggatcgcag gcttgacttc cttcattgcc accgctgcgc tcttctctcc
    16921 attgagccac gccataagtg cgtccgacca gacttggcca gcaacggcac caaggtttag
    16981 atcatacatt tcgcccttcc agttctcgac tccccgcttg actatttcag cggataccct
    17041 gtcggggcag ccagccttga tgctgccaga aatgtcgttg tcgtggtcct ctgcggtgaa
    17101 caggaagtcg gccaaagcca caaacgcctt cggatccttg gaattggcgg gaagaaccag
    17161 cgattccagc tgaaggttgg ggtatcgata atcagcagcg cgcggcccct tcggaacggg
    17221 cactatgccc cagcggtctt tcatcttcgg gcctatgaac tcgaatctcc agaactccaa
    17281 tggctgcatt gccactttgc ccgctacgaa ctggtcgccg ccccaatcat cgtcgaggca
    17341 tactttgtat ttctcataga ggtcaagata catagccatt ccttcagtga cgtttatctc
    17401 gtcgcctgtg aagattggac gtcccttgct gtcggcccgg gtcagcttgc catcgttcga
    17461 cagagccaca ccccaatgcc acagggacga gagaccccac tggtccgggt ttccatcgcc
    17521 gtcaaggtct ttggtggcct tgttggcaat gtcaaggcag tgatcccaat cccatttacc
    17581 ctgctcataa agcttctggg catccgggaa accttcacgc tcaaacatgg tcttgttgta
    17641 agccatgaat ctgaggcatc caaggatatt gccatagctg ccaaacccgt aagtgtttcc
    17701 accgtgcgac atgactttaa ccgcgccctg tcgcgctttg ggcaggctct tgtagtatga
    17761 ctgaggaaga accttgtcaa ggggacagaa ggcatctctg gccgccaatt gccagaagaa
    17821 gttgtgattc atgagccata catcatactc cgaatcacct gagagaagcc ttgtcatgag
    17881 caaagaggtg tagtcgtccc agttcagggg cttagctacg atcttgcagt tgaacttctt
    17941 ctcagcctct tggattctgc cttctctttc tttcccgggc gcaaacttgt caagatagtc
    18001 ccaccagccg acgaaagtca ccgtcttgcc ctttagatcg taagacgcag cggcgccagg
    18061 gcctggcagc ccagccagga ggccaactac caataccgcg cacgcgataa tccttgtcat
    18121 ccgcttcact ctttttacct ccctaaatct gctcatgcga ctatgctttg aactcgccaa
    18181 ttcaatcgac acggctaaat ctagctgatg ctgttgtctt caatgacact ccggccaatc
    18241 atcaccccca gttttgtgga ccaccctcag tccacagacc tctcagttta gccgactatg
    18301 cctgtgcgct cgacgccttc cacaaaatgc ttttgcatga cgaagtagag gatcaaaaga
    18361 ggcgccataa acagaagcat acccgtattg ttcacaagcg aggcatattc agcccgggcc
    18421 cccaaagtgt cagccagtgt ccgaagagtc cacggcagca acacctcatt gcccataaaa
    18481 actgatgtaa gtgtgctgtc attccactgg aacacgaatg agagcaggaa aatcgtcacc
    18541 atgcttggca cggccccagg caacataaca gtgaaaaacg ttctgagcgg actcgcccca
    18601 tcaacgtaag ccgcatcctc cagctcttta ggcacattcc tgaaatactg cctcatcacg
    18661 tatatgaaaa ggcccccacg catgccggct ccagtcattg ctatcaatat gaacggccaa
    18721 tagctgttga gaaggttgag accctggcct ggaatgaggc caaagagatt gaaatagcgg
    18781 aaattgagat acaacgggac gagtatcgtc tgcgaaggca ccagaagcgt taccaagaca
    18841 agggcaaata gaagattgga acccttaaac ttgaaccggg caaatccata gccaacaagc
    18901 gcgcttgaga atgtctgcag gaccgccact attaaggaga gcttgagcga attccagaag
    18961 gccgtcgggt atttcattgc ttcccataca accttgtagt tttcaagagt gaagtgcctc
    19021 ggaatccatc tgactgtgac atcgaacatg tcacgcactt ccatgaagga tgacgagatc
    19081 ttcgtcatca gcggcatcaa tatgacgtag caaatgccga ttattaaccc tgcccgaagg
    19141 atcctccaca ctatccggcc tgccttgcgc caaaaaggcg gtctcttcgc gtgctgctgc
    19201 cgaacttccc tttgtccggg cggcgctact cctctcactg cctccttggt tgcttccatt
    19261 tgcacaaccc tccacaccaa gaatgtgtgc tgacactatc gccttctgca gaccatcact
    19321 cctgataaaa cacccatttc gagacgatgg aaaccacaat tgcaactatg attccaacgg
    19381 cgagaaaata gagcagagcc atggctgtgc ccataccgta gctcgcccta aacgtgaaat
    19441 cctgaatcca gctgaggaat tcgctctggc ggcccgtaag gaaatctatt atcgtgtaga
    19501 ccaggttagt cataacaagt ggactcaaca ttgggaaagt tatgagccaa aaacgctccc
    19561 aacgcgtgca tccttccacg tcggccgctt catacagaga tccgggtatg gattgcaggc
    19621 ccgcgagaaa tatgaggatc tggatccccg atgacctgat aactactggg atgttgtcga
    19681 ctgcagaaac aatataggac atgaaccatt ccggaagctt gagctgccac agaaaagccc
    19741 tgatagcgct accgcctacc aagccctctg cgacagcggc ctgcgtcccc gcagcaactg
    19801 tctgcagtat gtaatccgtt ttctccatga aaaaaactac tccggaactc ataatcacgg
    19861 ggagaaagaa tatagttcga gcaacagaac gcccagggaa ttcctcgttc agcattacag
    19921 ctgcaaagaa gctgaatact atcaccaatg gcacttgacg cagcatcttg cctagttctt
    19981 caatgagttt cttgttgaaa tccggttgca ccaagatagc gtcatggaag ttctttaggc
    20041 caagatagtc gagttgatag cctgtcgaag tgattcgcaa ttcgctcaaa ctaaaggcaa
    20101 tggactgaat aaaggggaca agaaagagaa cgagaaatcc gatggtgaac ggaagtgtaa
    20161 acaaatagcc tgatacagcc cgtctgcgtt ctaaggtcag cctgcgcgtg gaatgtttca
    20221 cgtcacgccc ccctcaactt gcagcatctg aaggtcagac agttaatttc agggtgaaat
    20281 ctaatgtgat tatagcccct tctcttttac atgtcaagaa aagttcgtca tttctgggca
    20341 ttctacaccg gacagtgtcc gacaataaca catttacctg acgtttggca catccattgt
    20401 gtcaaaatca catcgtgacg agctcgggtt atctggctgg caagggccaa gaatat
//
LOCUS       DAOSYL010000044        20107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2204_length_20107_cov_8.232944, whole genome shotgun sequence.
ACCESSION   DAOSYL010000044 DAOSYL010000000
VERSION     DAOSYL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_20107_cov_8.232944"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1146..1652
                     /locus_tag="PLU15_07745"
     CDS             1146..1652
                     /locus_tag="PLU15_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30854.1"
                     /translation="MPKRNVLVVFLLVWVFAFSAVSYAADATNPYASDMQVVSVIVPE
                     VAEMTFITGNSFTMEFVWATDSWTLVAPDDVIHTLKYVTNVAGRRITLQAGIWNQNFY
                     GSNLHLSVTPDGGSPIFITGPSKDLINPVLLTGAYQEINLTYSAFADLTVIPGTYTFQ
                     IIYTMIDN"
     gene            1792..2652
                     /locus_tag="PLU15_07750"
     CDS             1792..2652
                     /locus_tag="PLU15_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30855.1"
                     /translation="MLYPNRRCVAGATTLALAVMLCVMMPPSAHASVNVLSGLTQMHE
                     VALGGEYSGVIVLENLGDVVERVELYQTDYRFTAEGYTYYEEPGTVRRSNAAWIRLGQ
                     SVVEIPPKSRASIPYRILIPNDTRLVGTYWSMVMAEMLGSVDEVDIDEPYKKDENAIV
                     LGVKQKFRYGIQIVTDIGGTGKKKVVFSNPALKRIESSGALTLSVDMANAGERLVNPS
                     VWVEVYDKSGTKVGRLEGKNAKIYPDTSVRATIELGALEPGSYKVIIVADNHDEYVAA
                     VRYSLEVPVP"
     gene            2658..5321
                     /locus_tag="PLU15_07755"
     CDS             2658..5321
                     /locus_tag="PLU15_07755"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOO30856.1"
                     /translation="MRRASLLPMIAFVLSAVCAFWAFAEESSALSIHPVNRLIQADPG
                     SVVTIVVAIENHSAEAVDVDLSSELPQSWFAFFGDETVRLAPGETEILPISAMIPKNV
                     LAGSYEILVHARYTCPRMNDRQSSEIRFTVLVNEVRSIRVTLVNAPDYVVDTAYTASF
                     LLSNHGNVVEDLKLSTCDIQGAYITLDPAWVSLEPGGSTPIDVHVILTAAPGRVLDHT
                     VLVKAGNRDLLSNWASASARVKVLPQKLSDSDAYHHFPLKIQLSAGVLGTSNRAQLKV
                     AGAGPLAHGGNDRLRFDLSDTGGVVEYRAPGMRLILGDGRFGLSPLTEWQAKARGIGV
                     QISQERAGLELYTHDGAGSPKVGLRAAATSSNGSSLSLQLLTQVEPATNILTVKGVHR
                     IPGVEIDCEYGMLANGSSSGAQALRASGRSKLGPVEAVVTVQNADRGYTAIDNPGIDM
                     SLKLKSLSKLPVSGTLLLQKHDDGELHSRKLEANAVWKLDSTELGLKYSDTAKWDDGS
                     DDLGRSRQASVSLNVFDSSNASLKEEISVQNCSGMLGNPGSWTVAYALKKQLIESRWK
                     WTSCLSASMPLSQGASVSVGLGADASYQVVPGLEMRGGFRAGTIRQSGLTVSAGATYK
                     PSDWAALSIDASCKIVDGGVRDLGVKVGQTAFFDIPLARRSDVGEISGKVVGVDGKGM
                     PGIIVNAGGFSTVTDSSGNFRFPAVVEGEQYVSVSVRELGPEYLVMPSSAVSVKVERN
                     TESTVQLTVARSGRIEGIVRFSSDEGSCVVPDEMIWRIADGISTQSAPKLLSGLLVEV
                     SGARRYVVPLDFEGRFAIDHLPPGDYRVTIDRSSVPSLYELTPSAMNITLGEGEKASA
                     EFAIYQVLREYDFTEGGEIGHELIVEGQQER"
     gene            5661..7298
                     /gene="pgi"
                     /locus_tag="PLU15_07760"
     CDS             5661..7298
                     /gene="pgi"
                     /locus_tag="PLU15_07760"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOO30857.1"
                     /translation="MSVTELPAWRALAEHRLEMENIHMRELFASDPERFDRFSIRLWD
                     LLFDYSKNRITDETMSLLFNLAEQARLAEAIEAMFSGERINTTENRPVLHVALRNRSN
                     RPIPVDGTDVMPEVNAVLSQMRRFSEAVRSGDWKGYTGKAITDVVNIGIGGSDLGPRM
                     VVHALKPYGHDRLRVHFVSNVDPTDISETLSDLAPETTLFLIASKTFTTQETMMNANT
                     ARNWLLRHAGEDAAVAKHFVAISTNRDAVEAFGIDDRNMFVFWDWVGGRYSLWSAIGL
                     SIALYVGMDDFESLLAGAHKVDEHFRTAPLDRNIPAIMGLLGIWYNNFWGAETCAVLP
                     YDQYLAYFPDYLQQADMESNGKSVTRTGGPVDYQTGPVLWGQPGTNGQHAFYQLIHQG
                     TKLVPCDFLAAAQSHNPIGDHHEVLVANFLAQTEALMKGRTSDETRAELAAQGCTGQE
                     LELLTAAKTFEGNKPTNTFLYKKLTPETLGMLIALYEHKIFTQGVIWNVNSYDQMGVE
                     LGKELASKILPELKDVRPAVGHDSSTVGLINAFKSMR"
     gene            7445..8701
                     /locus_tag="PLU15_07765"
     CDS             7445..8701
                     /locus_tag="PLU15_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30858.1"
                     /translation="MKRKAEKNGGVPVKFMSNIMAFIKRSESNVIAIRPNEYLFLCDE
                     NGHDMAALIAYSPEMKMDMLSEFKQDASAQYASGYSAVTGAIVDKLGAVGPVMMSGAR
                     AGELMQIVAPPEIVKALADGSVKLMQTPTGATSSVVAEGSSRIVGQVRLAPADVAPIV
                     APVVLYQILNAIAGAHQLSKINARLDSLQRAIDSVIFRQQANSYARLYAAIDALQELD
                     REYCAIGMFTDDMRWRLSIATNDIQAVNYESSFIIGRFKRMSEDLIRSSHKRQGAIQA
                     NTMLNEESNVYMLDAAIYVTAVKAGLMAEQMWIRHDLQFVPEYVGYRLADLNCEIEEL
                     KETVPPMRYIEELQDHADSCLREMRSLQRWTSPRLSKEIRSRSEQFASMQEDDSRQYT
                     AQSLFIWEDDKGEIKALSGVEAIDTK"
     gene            complement(9318..9860)
                     /locus_tag="PLU15_07770"
     CDS             complement(9318..9860)
                     /locus_tag="PLU15_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOO30859.1"
                     /translation="MPYPFNLSLRPETLSQVVFELLESCIVQGIDRFLLINGHGGNNS
                     AIDIALRRLGREYPSAKAAVSYAWWISGPRLCPPGTFGLENGLGHAGKAETAAGLAIV
                     PDLVDMDAARGEWPASPAEVSIKTTIPQISRTGATGDPTGATREQGEKMIGALSDYLA
                     RFLKEMDKTGWGFAREDWSF"
     gene            complement(9844..10029)
                     /locus_tag="PLU15_07775"
     CDS             complement(9844..10029)
                     /locus_tag="PLU15_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOO30860.1"
                     /translation="MSKVNEFRLEKLNRHRFEASHFNKAILVIGATENHGYHLPMGMD
                     TYAAYAIAERTADAVSL"
     gene            complement(10043..10819)
                     /locus_tag="PLU15_07780"
     CDS             complement(10043..10819)
                     /locus_tag="PLU15_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO30861.1"
                     /translation="MPEHDSTREWKSFFDIYAPYYMDEPFTLNTCREVDFIIEIMALE
                     PGASVLDVGCGVGRHSVELAKRGYQVTGVDISEGMLHEAARRAEQARVDVKWLRQDAT
                     ELKLDRTYDACICLCEGAFGLLNSGENAADRDISILRGIASALAPKGPFLLTALNALR
                     TIRMYSDDDVAAGRFDNIALCQIHPIGSFIADDIRNELPENILSSVIREKAFTPMELT
                     LMLNITGFSVEHIWGGTAGNWGRRPLLMDEIELMVMARLD"
     gene            complement(11250..13370)
                     /gene="feoB"
                     /locus_tag="PLU15_07785"
     CDS             complement(11250..13370)
                     /gene="feoB"
                     /locus_tag="PLU15_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOO30862.1"
                     /translation="MNHSSAHSRPVIAIAGNPNSGKTALFNAMTGSRQHVGNWPGVTV
                     ERKEGTLTRGETSALVVDLPGTYAFSSRALDEVIARNFIMHEHPDVAINVVDATNLER
                     NLYLTLQMLEMGVPTIVALNMIDEAEELGLDIDIDELSNHLGVGVVPTATVHGRGVEE
                     LVDLALKTANAVAGAEGLEDVEKPADAGTPSYWIDYGPILEKEIALLEQALAGVPDLC
                     RRRSARWLAIKLLEKDADILQEIRNSANEQANTAYGKLIAELEVAAEEACARVKKAAG
                     REAVDLIAEKRYELAASISASVIVSRPDDAEITPTQRIDKFVLHPWLAYPIFFVVVWL
                     MFQITFTLSEPIADALSGLFATFGTWLAGSLEAAGTPNIVIGLLVEGVVGGLGAVLEF
                     APPIFMLFLMISLLEDVGYMARAAVLSDRLMRSVGLHGKAFIPMILGFGCNVTGILAT
                     RSLDSKRDRLISILINPLISCAARLPVYVLFATAFFESRRGLVVFSLYALGVVLAFIF
                     AKVLSLFVEPDDEATFVLELPPYRMPRISGVLIQTWERGKEFLEKAGTVILLSVVLVW
                     ALSNLPPGVDPGSPDSLIGRIGKAIAPILAPAGFGQWQAAAALLFGVIAKELIVGTLG
                     VLYGTTGEAGLIAAIRASWTPLAAYSFMVMALTYIPCTATIAAIRRETGSRRWTVFAV
                     AYTLVLGWTLAVIIYQVGRLFGLA"
     gene            complement(13498..13890)
                     /locus_tag="PLU15_07790"
     CDS             complement(13498..13890)
                     /locus_tag="PLU15_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOO30863.1"
                     /translation="MSDANGAISLSQLNPGDRGTILSIDCEGPLRRRLMDMGLTPGTE
                     VCIEGTAPFGDPLIVCARGCRLGLRRGEAAGISVIPCADRPCSEACKLENHGPFRRRH
                     RHGQVAASGDKCLRESGRHQHRKADCTE"
     gene            complement(13887..14276)
                     /locus_tag="PLU15_07795"
     CDS             complement(13887..14276)
                     /locus_tag="PLU15_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOO30864.1"
                     /translation="MKNSTPISPSLEDYLEAVLEVADESGAARTTDIADKLGVSKASV
                     NQAMGLLVDRGLIEREKYGPVFLTDSGDEQARAVSRRHHTIKAFLVSVLGVAEHIAEE
                     DACQIEHVVSKDTMNGLVEFMERRDAE"
     assembly_gap    14857..14956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14971..15459
                     /locus_tag="PLU15_07800"
     CDS             14971..15459
                     /locus_tag="PLU15_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOO30865.1"
                     /translation="MPRPPKCRRVEFMPEVTVFKPAGVRLAEMEEEILLLEELEALRL
                     KDVEGLEQEECADRMQVSRPTFQRVLSSAREKVARALVEGKAIRIEGGSYRLAARKLR
                     CEDCSYEFEAPVAMYRQGAGAACPRCGARHGRRNDGDQTAGGMMHHGRCGRGWKNTEG
                     DE"
     gene            15579..16547
                     /locus_tag="PLU15_07805"
     CDS             15579..16547
                     /locus_tag="PLU15_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO30866.1"
                     /translation="MKYIFGPVPSRRLCRSLGVDLIPYKHCSFDCIYCQLGATVHKRA
                     ERQQFVETGEVIAELESALPCVEADYVTLSGSGEPTLCLGIGELIEGIKELTEIPVAV
                     LTNSSLMSCAEVRKELLGADLVIPSVDAATQDVFERINRPCSGLRIEDILCGLEEFSY
                     EYSGKLWIEVMMVRDINDSDDEIDKIASALTRIRCEKIQLNTVERPPAESFARALTQG
                     ALEKARRRFDDRAELIVASGIYQDADGSFHSPAGAGLGAPGIEADCGRVLELLKRRPC
                     TVSDIAEGLDLNINEAAKFVGILKGEGLICAIRREGRAYFFANDGN"
     gene            16760..17170
                     /locus_tag="PLU15_07810"
     CDS             16760..17170
                     /locus_tag="PLU15_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOO30867.1"
                     /translation="MPRGDGRGPTGFGPMTGRGAGFCTGYDVPGYANPAMGRGPGFGR
                     APRCYGGGGFGRGYRRMYYATGLPRWMRSGAGAWGQAAYGPAYSPYAQEAMRPSKEQE
                     IGYLKRQSEFLMEELEAVRGRLSELSGSQEYDEG"
     gene            17295..17681
                     /locus_tag="PLU15_07815"
     CDS             17295..17681
                     /locus_tag="PLU15_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO30868.1"
                     /translation="MKLAVTAQGTSLSAGLDPRFGRCEHFVIIDVDTEEFEAFSNESV
                     MASGGAGTESAQFLADKGAEAVVTGNVGPNAAMALEAAGIKVYRMSSGTVKDALEAFK
                     SGTLEAISGPTVNSHHGMGGHGRGGR"
     gene            17795..18202
                     /locus_tag="PLU15_07820"
     CDS             17795..18202
                     /locus_tag="PLU15_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO30869.1"
                     /translation="MKIAVATDGSTVSPHFGHCSEFTIVTVENGKAVDSAILPNPEHQ
                     PDFLPQYLSEHGVSCIIAGGMGPRAQQHFAARGIKTVIGASGSVDDVVHAYLAGSLEV
                     GESACDHGHGHEQHGECGHGENGGTHRRGCGHN"
     gene            18300..19199
                     /locus_tag="PLU15_07825"
     CDS             18300..19199
                     /locus_tag="PLU15_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO30870.1"
                     /translation="MIVSIASGKGGTGKTTVAVNLALALEGAMPIRFLDCDVEEPNAH
                     LFLHPEIERSEKVSLPVPVVDESKCNGCGKCARVCAFHAILAFSSRTIVLPELCHGCG
                     GCALLCPQGAITESPKVIGEVETGRAGDIRFAHGRTGIGSPLAPPVVKAVREALDKEG
                     LGAGLTIIDAPPGTSCPVVASIKGSDYCVLVTEPTPFGLNDLGLAVGLVRELGIPCGV
                     VINRAGLGDEAVSAYCSREGIPVLLEIPYDRKYAECYAKGGRLVDDFPELRGALKGLW
                     RKISASGESGAEGEDGRREMGEI"
     gene            19196..>20107
                     /locus_tag="PLU15_07830"
     CDS             19196..>20107
                     /locus_tag="PLU15_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO30871.1"
                     /translation="MKELLVISGKGGTGKTSVVASFAALAENKVLVDCDVDAADLHLL
                     LSPEVVESHEFMASRQATVDVSRCTGCGHCSEVCRFGAIAMAYGHPTIDPISCEGCTV
                     CSHICPEGAITMVDVMSGHWYVSHTRFGPMVHAKLKAAEENSGKLVSQVRRKAREIAE
                     SQGHELIITDGPPGVGCPVISSISGVDLALIVTEPTVAGVHDMERVLDLTCHFGVESA
                     VCINKCDLDEANAERIEDYCCARGIEIAGKIPFDEDMVRAVVRGEPPVLYGDGAPDCA
                     GPDSVAGEAAQAIRQLWDRVAVLLARDA"
BASE COUNT         4491 a         4970 c         5923 g         4623 t
ORIGIN      
        1 ctcggtgcgg cgaacttcac gcctgccctt gaggagtttc cgagcagatt cctagtcttt
       61 gctatgggga ttatcacaca cagaacagcg cttgcctatg tgggcgctgc ttgacgctta
      121 ctttggaagg actcgtgggt tatcggttat tctcgatccg tgtggacccg catcactcaa
      181 acttctatcc tacacacatt ccaccccaag gtatcagcgc gaactccaca tactcagttt
      241 ctattacgct gttttgctga atagcttgat aactatccct gaacaatcta aaggcgctag
      301 aagcaagagc ccatcatctg tttggcaatc gaaaagttca tagttcggca gtcaaaaagt
      361 gcataggcta ccaccacgcc cggggtcgga tctcagggcc ctattgggcc gggtcgtttt
      421 tgtaaatagt gttttgttgg gggtgggcac ctccaacgtc atctgggaca tgcaattggg
      481 agagggcgtg ccgtagacgg tagctttccc ctgtgaatgc aagctcacat tccaagtgcg
      541 tcccgcctgt ctcttgggtc ttcttatcgt tccctgcgtt cgtcgcgtaa tttgttggat
      601 tgcatgatat atctgaaaaa gagaagcata tgtatgccat gattgccttg caatggttta
      661 tgctctgccc tataggctgt tttgatgaga gggtgctcga gcctgttgcc atggggctgt
      721 aatcatatta cctgcacggt tgagatgtgc gaggtgaccg tcatcagtcg acaccgtcta
      781 cggcaagcag cgctgattgc acagaaaaac taagcaaaat cgcgtgcttt cggtatcgag
      841 ggatcgatta gatatggaac ggtttcgtat acttccgctt tgcacgcaaa gctggtggaa
      901 tattacataa aagacaggac gtgcgaatcg ccaggcagac ctgattattt cggcgtgcgt
      961 gtatcggaat gcctcgtttg ggagtccata ggattcgaca acctgcccac ggaatctgct
     1021 gtagactctg gttggtttca tcctgggcca gcacgactca atggggtctt tcaaagatgg
     1081 gtgggcagag ttgccaactg cctgccttaa gtcacaaata atcacaaaca ggaggttttt
     1141 tgcgaatgcc aaagcgaaac gtcttggtag tatttctcct agtgtgggta tttgcatttt
     1201 ccgcagtatc gtatgctgcg gatgccacga atccctatgc ctcagatatg caggtcgttt
     1261 cagtaatagt cccggaagtc gctgagatga catttattac cggaaacagc ttcacaatgg
     1321 aattcgtttg ggcaacggac tcatggactc tggtagcccc tgatgatgtt atccatacgc
     1381 taaaatatgt cacaaacgtc gctggtcgaa ggattacgtt gcaggcggga atctggaatc
     1441 agaatttcta tggcagcaac ctacatctga gcgtgacacc ggacggagga agtccgatct
     1501 tcattacagg gccctcgaaa gacttaataa accccgtgct gttgacagga gcatatcagg
     1561 aaattaacct aacctactct gcttttgcag acctcactgt catcccgggt acttatacat
     1621 tccagataat ctacacaatg atagataact gagttagcct agttggatca cgaaaggctc
     1681 atggaggccg gggttcttcc tcggcctttc ccatcggcag ccctgtgcgg gtactggctg
     1741 aagagggagg atgtctctgt gcacggttga agcgaaggga gcgatgttgt catgctatat
     1801 ccaaatcgca gatgtgttgc aggtgccact actttggctc tggctgttat gctttgtgtg
     1861 atgatgccac ctagcgccca tgcctcggta aacgtgctga gtggcctcac tcaaatgcat
     1921 gaggttgctc taggcgggga gtatagcggg gtcatagtac tggaaaatct gggcgatgtg
     1981 gtcgagaggg tggagcttta tcaaacagat taccggttta ctgcagaagg ctacacatat
     2041 tatgaagagc ctggcactgt tcgacgctcc aatgctgctt ggattcgcct gggacagtct
     2101 gttgtagaga ttccgcccaa atctagagct agtatcccct acagaatcct gatacccaac
     2161 gatactaggc tcgtcggtac ttactggagt atggtgatgg cagagatgct gggcagtgta
     2221 gacgaggtcg acattgacga gccttacaag aaggatgaga atgcgattgt cctaggagtg
     2281 aaacagaagt ttaggtacgg catccagata gtaaccgata tcggcggtac gggcaagaaa
     2341 aaagtagtct tcagcaaccc ggcactcaaa cgcatcgagt cttccggcgc ccttacattg
     2401 agcgtagaca tggccaacgc cggcgaacgg ttagtcaacc cgtctgtatg ggttgaggta
     2461 tacgacaaga gcggaacgaa ggtaggacgg cttgagggca agaacgccaa gatatacccc
     2521 gacacgtctg ttcgggcaac gatcgagctc ggcgcgctcg agcccggaag ttacaaggtg
     2581 attatagtgg ctgacaatca tgacgagtac gtggcggctg ttcggtacag cctggaggtg
     2641 ccggtgcctt gatcggtgtg aggagagcca gcctgcttcc gatgattgct tttgtgctct
     2701 ccgccgtctg cgctttttgg gcattcgctg aagagtcgag cgcgttgtcg attcatcctg
     2761 tcaacagatt gattcaggca gatccgggca gtgttgttac cattgtggtg gcgattgaaa
     2821 accacagtgc ggaggcggta gatgttgacc tcagttcgga actgcctcaa tcgtggtttg
     2881 cgttcttcgg agatgaaacg gtcaggctgg cgccgggcga aaccgaaatt ctgcccatat
     2941 cggcaatgat tcccaagaat gtgttggccg gaagctacga aatcctcgtc catgcgaggt
     3001 acacatgtcc gcgcatgaac gatcgtcaat cctcagaaat ccggttcact gtgttggtaa
     3061 acgaagtcag gagtatcaga gttacacttg tcaatgcgcc ggattacgtt gtggacactg
     3121 cttacacagc atcatttctc ctgagcaacc atggcaatgt cgtggaggac ttgaagctct
     3181 cgacctgcga tattcagggg gcgtacatca cactcgaccc tgcctgggtc agcttggagc
     3241 cgggcgggag cactccgata gatgtgcatg taatcttgac tgctgctcct ggaagggtcc
     3301 tcgaccatac agtacttgtc aaagcgggaa accgggattt gctgtcgaac tgggcgtcgg
     3361 ccagcgctcg ggtgaaggtg cttccgcaga agctgagcga ttccgatgcc taccaccatt
     3421 tccccctcaa gatccagctg agcgcaggtg tgcttgggac atctaatcgg gcgcagctca
     3481 aagtggccgg ggcaggcccg cttgctcacg ggggcaatga cagactacgc tttgatctat
     3541 cagatacggg aggggttgtc gaatacagag ctcctggaat gcgtctgata ttaggtgacg
     3601 gcaggttcgg cctgtccccg ctgaccgaat ggcaggccaa ggcgcgaggc ataggcgtgc
     3661 aaatcagtca ggaaagggcg ggactcgagc tctatactca cgatggcgct ggctctccta
     3721 aggtggggct tagggctgcc gcgacttcta gcaacgggtc ttcgctttcc cttcagcttt
     3781 tgacacaagt agagcccgcg acgaacattc tcacagtaaa aggtgtgcac aggatacctg
     3841 gcgtcgaaat cgattgtgag tatggaatgc tggctaatgg gtcgagttct ggcgcacaag
     3901 cgctgcgtgc aagcggcagg tcaaaacttg gcccggttga ggcagttgtg accgttcaga
     3961 atgcggatcg tggctacacc gcaatagaca atcccgggat tgacatgtcg ttgaaactca
     4021 aaagtctctc aaagctgccc gtatcaggca cccttctgct gcaaaagcat gatgatgggg
     4081 aattgcatag ccgcaaattg gaagctaatg ccgtatggaa gttggattcg actgaactag
     4141 gattgaaata ctccgatacg gcgaaatggg acgacggttc ggatgatctc ggcaggtcta
     4201 gacaggcctc agtctctctc aacgtcttcg atagcagcaa tgcctctctc aaggaggaga
     4261 tttctgtgca gaactgctcc ggcatgttgg gaaaccccgg aagttggacc gtggcatatg
     4321 ctctcaagaa gcagttgatt gaaagcaggt ggaagtggac ctcctgcctg agtgcgagta
     4381 tgccgttgag ccaaggcgct tcggtaagtg ttggcttggg tgcagacgct tcatatcaag
     4441 ttgtgcccgg gctggagatg aggggcggtt ttcgcgcagg cactataagg cagagcggct
     4501 tgactgtatc tgcaggagcg acgtacaagc catctgattg ggcggcccta tcgatcgatg
     4561 catcctgcaa gatagtggac ggcggggtgc gtgacttggg agtgaaggtt ggccagacag
     4621 ccttcttcga tattccccta gcccgccgct cagacgtggg ggagatatcg ggaaaggttg
     4681 tgggcgtgga cggtaagggt atgccgggta ttatagtgaa cgcgggaggg ttttcaaccg
     4741 taacggattc atcgggtaat ttcagattcc ctgcggtggt cgaaggcgag cagtatgtat
     4801 cggttagcgt gcgtgaactg ggccccgagt atctagtgat gccctcgtcc gccgtcagtg
     4861 tgaaggtgga acggaacacc gaatccacag tgcaattgac tgttgccagg tcgggaagga
     4921 tcgaaggtat tgtcagattc tcttctgacg agggaagctg tgtagtgcct gatgaaatga
     4981 tatggcgtat tgcagatggg atatcgactc aaagtgcgcc caagctgctg tcagggctgc
     5041 tggtggaagt atccggagct aggcggtacg tggttccgct tgactttgag ggccggtttg
     5101 ctatcgacca tctcccccca ggggactaca gggtaactat agatcggagc tctgtgcctt
     5161 ccctttacga gctgactccg tcagcgatga acataacatt gggcgaaggt gaaaaggcat
     5221 cggctgaatt tgctatctac caggtacttc gggaatatga tttcactgaa ggtggtgaga
     5281 ttggacacga gctaatagta gagggacagc aagaaaggta gttcagggaa attgcgcggg
     5341 agttgaggtg atcaggcgac gcagcggtat ctgctaatag gggcaaaccg cgttctgcat
     5401 gtggtatgtc tgcgaagaag gcctttcatc aaaatgggac gtgccatacc tatcctccat
     5461 atgagcaacg ccgacaaggc gttagcctct ccagatcacg gtgttcagct cgagggctgt
     5521 accgggtttt gcctaagaaa tagccttgtt cccatgctac acccggggta tgataagaat
     5581 tggcatgaaa tgatgcagtt cgcttgaatg gctccctcgt ttttgaagag ccgagctgtc
     5641 ttatggggag gaacaccacc atgtctgtaa cagaacttcc ggcgtggcgg gccttggctg
     5701 agcaccggct ggagatggaa aacatccata tgcgcgagct gttcgcatct gacccggaga
     5761 gattcgaccg attttcgatc aggctttggg atttgctctt cgactactcc aagaaccgca
     5821 ttacagacga gaccatgtct ttgctattca accttgccga acaggcccgc cttgcagagg
     5881 caatagaggc gatgttcagc ggcgagcgga taaacacgac tgagaatcgc ccagtacttc
     5941 atgtagccct gaggaatcga tccaatcggc ccatcccggt ggatggcacg gacgttatgc
     6001 ctgaggtaaa tgcggtgctt tctcagatgc gccgcttttc tgaggcggtg cgcagcggcg
     6061 actggaaggg ctataccgga aaagccatca ccgatgttgt caatatcggg attggaggat
     6121 cagatctggg tcctcgcatg gttgtgcacg cgctaaagcc ttacggccat gaccgcttgc
     6181 gcgtccactt tgtgtcgaat gtagatccca cggacatttc agaaacccta tccgaccttg
     6241 ccccagagac gacgctcttc ctcatcgctt cgaagacttt cactacgcaa gagaccatga
     6301 tgaacgccaa tacggctcga aattggcttt tgcgccatgc cggagaagat gcagctgtag
     6361 ccaagcactt cgtggcgatc tccaccaatc gcgacgcggt tgaagcgttc gggatcgatg
     6421 atcgcaacat gttcgtcttc tgggattggg ttggcggccg ttactcgctc tggtcggcca
     6481 ttggactttc gatagctctc tatgtgggca tggacgattt cgaatctctg ctcgcaggcg
     6541 cgcacaaagt agacgagcac tttcgcactg cgcccttgga ccggaacatc ccggctataa
     6601 tggggttgct cggcatatgg tacaacaact tttggggtgc cgagacctgc gctgttctcc
     6661 cttacgatca gtacttggct tatttccccg attacctgca gcaagcggac atggaaagca
     6721 acggcaagag cgtgaccagg acgggggggc cggtggacta tcagactggg ccagtacttt
     6781 ggggacaacc aggaaccaac gggcaacacg ctttctatca gctcattcac cagggcacga
     6841 agctggtccc gtgcgatttc ctggctgcag cacagagcca taatcccatt ggcgaccatc
     6901 acgaagtgct tgtagccaac ttcctggcgc agacagaggc gctcatgaag ggccgaactt
     6961 ccgacgagac ccgggcggag cttgcggctc aaggctgcac tggtcaagag ctggagttgc
     7021 tgaccgctgc gaagaccttt gaaggaaaca agcctacgaa cacgttcctt tacaagaagc
     7081 ttactcctga aacccttggc atgttgatcg ctctgtacga gcacaagata ttcactcagg
     7141 gcgttatttg gaatgtgaac agttatgacc agatgggggt cgagctaggg aaggaactcg
     7201 ccagcaagat tttgcccgag ctgaaggacg tcaggcccgc agtcggtcat gacagcagca
     7261 ctgtcgggct aatcaatgcg ttcaaaagca tgagatagcc gatgatgtca tccgtttggc
     7321 agccggagag ttcgtagttc ggcggtcaaa atgtgcatag ctaactatgc ctggggccag
     7381 tggttgctct ccatttttag ccatatatgc atgcaagcct gtgcgagttg gcggcgaagc
     7441 atccttgaaa agaaaagccg agaagaacgg aggcgtgccg gtgaagttca tgtcgaacat
     7501 tatggccttc ataaagaggt cggaatcgaa tgtgatagca atcaggccca atgagtacct
     7561 gtttctttgt gatgagaatg gacacgatat ggctgctctg atcgcgtatt cgcctgaaat
     7621 gaagatggac atgctaagcg agttcaaaca agacgcaagt gcccagtatg cgtccggtta
     7681 cagcgcagtg actggggcga tagtcgacaa gcttggcgcg gtgggaccgg tcatgatgag
     7741 cggggcgaga gccggcgaat tgatgcagat tgttgcgccc cccgagattg tgaaggcttt
     7801 ggcggatgga tcggtgaagc tgatgcagac gccgactggg gctacaagca gcgtggtagc
     7861 tgagggatcc tcgagaatag tgggccaggt taggttagcg ccggcagatg ttgcgccaat
     7921 agttgctcct gttgttctct atcaaattct aaatgccatt gcaggtgcgc atcaactgag
     7981 caagatcaac gctcgtttag acagccttca aagggctata gattccgtaa tcttccgtca
     8041 acaggcgaac tcatatgccc gactctatgc cgccatcgat gcgttgcaag aactggatag
     8101 agagtattgc gctatcggaa tgtttacgga tgacatgcgc tggaggcttt ctatagctac
     8161 gaatgatatt caggctgtaa actatgaaag cagctttatc attggccgct ttaagcgcat
     8221 gagcgaagat ctgataagat catcccataa aaggcagggt gcgatacagg cgaatacaat
     8281 gctgaacgaa gagtcgaatg tgtacatgtt ggatgcggcc atatacgtga ctgcggtcaa
     8341 ggcggggctc atggctgagc agatgtggat aaggcacgat cttcagtttg tccctgaata
     8401 tgttgggtat aggcttgccg acttgaattg cgagattgaa gagctgaaag agactgttcc
     8461 gccaatgcgt tatattgagg agcttcagga tcacgcggat agctgtttgc gggaaatgcg
     8521 gtcgttacaa agatggacca gcccgcggct gagtaaggag atacgaagcc gctctgagca
     8581 atttgcgtca atgcaggaag atgattctcg gcaatatact gctcaatcgt tgttcatatg
     8641 ggaagacgat aagggcgaaa tcaaagcctt atccggagtg gaggcgatcg atacaaaata
     8701 gacgttaccg ctgccacagc gcttcggttc ggctacagtt cgtctgcttc gtcgatgttg
     8761 catgcgatgc aaaactccgc agtgtgatga ggaccctctg acggttggcc attcgacggg
     8821 cactgcaacc ggatcataat tctctttgcc gtattgcata gaaaggccat tgatataata
     8881 gtgtgtgccg cttcccagct ggacgccatg ggcagaatcg atgtggccag agcaaaagga
     8941 atgccaaatg ggatggcgag ttggctcccg atcatcctat ttccaaagat cgggatggat
     9001 gtctcttggt caagccctgc gtcttttgga atatcgtagt cctcacggaa tggcgggcta
     9061 cgcgaatctg cccaaaaaca gtgaacgagt ctgatcgtat tagggaagtc aatgccacaa
     9121 aatgtttcat gtgaaacatt tgaccatgta ttatgcacat agatgtccag aaagctctgc
     9181 ttttcatggc ataaactcgg tctgctcccg tctggcacct gggcgagttg ttggcctcag
     9241 caaggagctg aggccaaagt gtgggcagtc cggacgcata tgccctccta acatgagcgt
     9301 tcggggacgc tgcacggtca gaacgaccag tcttccctgg cgaagcccca gcctgtctta
     9361 tccatctcct tgaggaatct cgccagataa tctgagagag ctcctatcat cttctcgcct
     9421 tgctcccgcg tggcgccagt gggatccccg gtcgctccgg tcctggagat ctggggtatc
     9481 gtggtcttga tgctcacttc agccgggctg gccggccact cgccccgtgc tgcatccata
     9541 tcaaccaggt caggcacgat cgccagccct gcagctgttt cggctttgcc ggcgtgccca
     9601 aggccgttct ctaggccaaa ggttcccggc ggacacaggc gcggccccga tatccaccaa
     9661 gcgtacgaca ctgccgcctt ggctgaagga tactctcggc caaggcgtct caaggcgatg
     9721 tcgatggcag agttgttccc gccatggccg tttatgagga gaaaacgatc gatgccttgg
     9781 acgatacagg actccagtaa ttcgaagact acttgggaga gggtttcagg acggagactt
     9841 aagttaaagg gatacggcat cagcggtccg ttctgctatg gcgtatgcgg cgtaggtgtc
     9901 catgcccatg ggaagatgat agccgtggtt ctcggttgcg ccgataacaa gaatagcttt
     9961 gttgaagtgg gatgcttcga acctgtgccg gttgagcttt tccagtctga attcgtttac
    10021 ctttgacatc tgcgctgcct cctcagtcaa gcctggccat gaccattaac tctatctcat
    10081 ccatcaaaag aggacgacgc ccccaatttc ctgcagtgcc gccccagatg tgctccacgc
    10141 tgaaccccgt tatgttcagc atcagcgtca gctccattgg ggtgaaggct ttctccctta
    10201 tcacggagga taatatgttt tccggcagtt cgttgcggat gtcgtcggct atgaacgagc
    10261 ctatgggatg gatttggcac aaggcaatgt tgtcgaatct tcctgcggct acatcatcat
    10321 cgctgtacat gcgtatagtc ctgagggcgt tgagagcagt aagaaggaag ggaccctttg
    10381 gagccagggc tgaagcgatg cccctcaata tggagatgtc gcggtctgcg gcgttttcgc
    10441 ctgagttgag gaggccgaaa gctccttcgc acaagcatat gcatgcatcg taagtgcggt
    10501 ccaatttgag ctcggtggca tcttggcgca gccacttcac atcgactctc gcctgttctg
    10561 ctctgcgggc agcctcatga agcattcctt cggatatgtc tacgccagtg acctgatatc
    10621 cccgcttggc aagttcaacg ctatgccgtc cgacaccgca acctacgtcg agcaccgatg
    10681 cgccaggctc aagggccatt atttctatta tgaagtcaac ctctctgcag gtgttgaggg
    10741 tgaaaggctc gtccatgtaa tagggcgcat agatgtcgaa gaagcttttc cattcacggg
    10801 tcgaatcgtg ctcaggcaaa gctattcctc ctcgcgttat ctttaccctg tttgttctgt
    10861 gtgcggaggt ctcttcctgc atcgccctga cgatgttgac accgttgtgc caagggcaat
    10921 ccgggattcc cctgagctcg atgagcagct agccgaggca accgggcgtt ttgaaaggtg
    10981 tatcgcccta gaggttttgt tagagtatgg agtcaagttc gacaggaagt tgatcggaac
    11041 gagaacccta gggataagcg cggcaaggtt tggtttgatt gggccagtgc cagatgcgcc
    11101 tgggatgctt cgtggcctcc gagctatgtg ccaggcataa tctctcaggc gtttgctgat
    11161 gatcgaagta ctgttggcgc cttcgggccg ctgtgcatcg acgaaggagg ccagaggcgg
    11221 cagccgcctc tggctcggtt acgagctgct caggccagcc caaaaaggcg acccacttga
    11281 tagattatga ctgcaagcgt ccacccgagc acgagagtat atgccacggc gaaaaccgtc
    11341 catcgccgag aacctgtctc cctgcgtatg gccgcaatcg tggctgtaca cggtatgtat
    11401 gtgagggcca taaccatgaa tgaataggcc gcgagcgggg tccagctggc tctgatcgca
    11461 gcgatgagtc ccgcttcccc tgttgtgccg tagaggactc ccagggtgcc gacgatgagc
    11521 tccttggcta tcaccccaaa gagtaaggcc gctgctgctt gccattggcc gaagcctgcg
    11581 ggggcaagaa tgggagctat tgcctttccg attctcccta tcagcgaatc cgggctgccg
    11641 ggatcgacgc ctggcggcag attggacaat gcccatacca ggacgactga gagcaggatg
    11701 actgtgcctg ccttctccag gaattccttt cccctttccc acgtctgaat caggaccccg
    11761 gatattctcg gcatgcgata cggcggcagt tcaaggacga atgtggcctc gtcatccggt
    11821 tccacaaata ggctcaatac cttggcaaag atgaaagcta gcacaactcc gagggcgtac
    11881 aaggagaaga caaccagtcc gcggcggctt tcgaagaaag ctgtggcgaa cagtacatac
    11941 acaggtagac gcgctgcgca ggatattaga gggttaatca atatggatat aaggcggtcg
    12001 cgcttgctgt ccaggcttct ggtggccaga atgcccgtga cgttgcatcc gaacccgagg
    12061 atcatcggga tgaaggcttt cccgtgaagc ccgactgacc gcatcagcct gtctgatagc
    12121 actgcagccc ttgccatgta gcccacgtct tcaagaagtg agatcatcaa aaaaagcatg
    12181 aatattggcg gtgcgaactc caatacggcc cctagtcccc cgactacgcc ttctacgagc
    12241 aagccaataa cgatattggg tgttcccgcc gcttccagag aacctgcaag ccaggtgccg
    12301 aatgttgcaa ataagccgct gagtgcgtct gcaattggct cgctgagggt aaatgttatc
    12361 tgaaacatta gccatactac gacgaagaat atgggatatg cgagccacgg atggagcacg
    12421 aacttgtcga tacgctgcgt aggagtgatc tcggcgtcat ctgggcgtga gactatgacg
    12481 gacgcggaga tgcttgccgc aagttcgtag cgcttttcgg caatcaagtc cacagcttca
    12541 cggcctgctg ccttctttac gcgcgcgcag gcctcctctg ctgcgacttc gagttcggca
    12601 attagctttc cataggccgt gttggcctgt tcattcgctg agttcctgat ttcctgcaaa
    12661 atgtcggcgt ctttttcaag cagctttatg gccagccacc gcgcagatcg tctacggcag
    12721 agatccggga cgccggcaag tgcctgctcg agcagggcaa tttccttctc taaaatggga
    12781 ccgtagtcta tccagtatga aggcgttccg gcatcggcgg gtttttctac atcttcgagg
    12841 ccttcagccc cggcgaccgc attggcagtc ttgagggcga gatcgactag ttcctcgact
    12901 cctcttccat gaacggtagc tgtgggcacg accccgactc cgaggtggtt cgacagctca
    12961 tctatgtcta tatcgaggcc gagctcttca gcctcgtcta tcatgttcag tgccacaatg
    13021 gtgggcacgc ccatttccag catttgaagg gttaggtaga ggtttctttc gagattcgta
    13081 gcgtcaacga cgtttattgc cacgtctggg tgctcgtgca tgatgaagtt acgggcaatt
    13141 acctcatcca gcgctcgcga gctgaaagcg taagtacccg gcaggtccac gactaatgct
    13201 gaggtttcgc ctcttgtcaa tgtgccctct tttcgctcta ctgtgactcc gggccaattg
    13261 cccacgtgtt gtcttgaccc tgtcattgca ttgaaaaggg ctgtcttccc tgagttgggg
    13321 ttgcccgcaa ttgctatgac aggcctagag tgtgcacttg aatggttcat ctcattgtgc
    13381 ctccgtagat gattcgtgta aatgatgtat aaagcctaaa agacgcataa tagttaccaa
    13441 cgcctaactt ttggtccaaa aagagtgcgc ccaatggacg cccgtgcgag tatttctcta
    13501 ttcagtgcaa tctgctttcc tgtgttgatg gcgcccgctt tcccgcaggc atttatcgcc
    13561 tgaggccgcc acctgtccat gtctgtggcg tcgacggaac ggcccgtgat tttccagctt
    13621 gcatgcttcc gagcagggcc tgtcagcaca cggtatgacg gatatgcctg ccgcttcgcc
    13681 tcttcgcaga ccgaggcggc agccgcgggc acacactatc aggggatctc cgaagggcgc
    13741 tgtcccttct atgcagactt ctgttccagg ggtcaagccc atatccatca accgtctcct
    13801 gagtggtccc tcacaatcta tgctcaggat tgttccccga tcgcccggat tgagttggct
    13861 gagggatatt gctccgttag catcgctcat tccgcgtctc tcctctccat gaattcaact
    13921 agtccgttca ttgtgtcttt gctgactaca tgctctatct ggcacgcatc ttcttctgct
    13981 atgtgttcgg ccacgcctag caccgatacg agaaaggctt tgatggtgtg gtggcgcctg
    14041 gacacggctc gagcctgttc gtccccggag tctgtgagaa agactggacc gtacttttcg
    14101 cgctcaatta ggccgcgatc gaccaaaagc cccattgcct gattgacgct ggctttcgat
    14161 acgccgagct tatcagctat atcggtggta cgggcagcac cgctttcgtc cgccacttcc
    14221 agcacggctt cgaggtaatc ctcgagcgat ggcgatattg gcgttgagtt cttcacttct
    14281 ttctcccacc ttcatactga ggcggccgat ctggatcagc cgaaagctaa gtaaggcatg
    14341 actaacttgc cctgataaga atctatgcca tttttcagga gatgtcaaga gctttctgac
    14401 gcgtattgac atgtacgtgc cgtagcggga aaataattat ggacatatga tcaatatgca
    14461 acaatataat ctacccgtct cgactggcct ttatcaactc tatccaggtg cgatacgtcc
    14521 tgggacggga aggcagtttg aaggactcat gtgcgttcag tgcaggttag ccatagtcaa
    14581 ttgcgtgctc aggggatggg tgtagatggg atgagagggc acctggttgt ctctttgcaa
    14641 gttgcagttg agcctggggc tttcctttgt agccttaaaa catcgccttg ggcgactata
    14701 tcgcacgcgg gccctaaccc caaggaacgt gaatttcagg atcccgcaat attcgattgt
    14761 aagcggctca gattgctgtt gcgccgattg aagaaaactt gtaggagctg atattatgcc
    14821 gaggcctcca aaatgcagaa gagtcgagtt catgccnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnntagg agctgatatt atgccgaggc ctccaaaatg cagaagagtc
    15001 gagttcatgc ccgaagtgac tgtcttcaag cctgctggcg ttcgattggc ggagatggaa
    15061 gaggagatcc tcctcctgga agaactggag gctttgcgcc tcaaggatgt tgaagggcta
    15121 gagcaagagg agtgcgccga taggatgcaa gtgtcccggc ctacattcca gagggtgctc
    15181 tcttcggcca gggagaaggt ggcccgggcg ctcgtggaag gtaaggctat ccggattgaa
    15241 ggcggaagct accgtcttgc tgcgcggaag ctgcggtgcg aggattgcag ttatgagttc
    15301 gaggcacccg ttgccatgta tcgccaaggc gccggagcgg cttgccctag gtgcggtgcg
    15361 cgtcatgggc gacgtaacga cggggaccag acagcaggcg gcatgatgca ccacgggcgc
    15421 tgtggcaggg ggtggaagaa taccgaaggc gacgaataga ggttaaagct tcaaggtgcg
    15481 tccaaagcgg ccacatgcca ggcaactgac ctttacgcct tgccaaaaac ttcgccgggg
    15541 ccaaatgagg caaccagagt atacgggggg cgttgagctt gaaatacatc tttggtccag
    15601 ttccctcaag acggctgtgc cgttcgttgg gggtcgactt gattccgtac aagcattgtt
    15661 ccttcgactg catctattgc cagctcggcg cgaccgtcca caagagggcg gagcgccagc
    15721 aattcgtcga gacaggcgag gtcatcgccg agctcgagtc ggctttgccc tgtgtggaag
    15781 ccgactacgt cactttgtcg gggtcgggcg aacccaccct atgcttgggg ataggggagc
    15841 tgatcgaggg gataaaggaa ttgacagaga ttcctgtggc ggttctgacc aatagctcgc
    15901 tcatgtcatg tgctgaggtc cgaaaagagc tccttggcgc ggaccttgtg attccttctg
    15961 ttgatgccgc aacgcaagac gtttttgaaa ggataaaccg cccatgctcc ggattgagaa
    16021 tcgaggacat cctctgcggc ttggaggagt tctcgtatga gtactcgggg aagctttgga
    16081 tagaagtgat gatggtcagg gacataaacg acagtgatga tgaaatcgac aagatagcct
    16141 ctgccctgac acgcataagg tgtgaaaaga tccagctcaa tacggtggaa aggcctcccg
    16201 ccgaatcctt tgcccgcgca ctgacacagg gggcgctgga aaaagcgaga agacggtttg
    16261 acgatcgggc ggagttgatc gtggcgtccg ggatttatca ggacgctgat gggtcttttc
    16321 atagcccagc cggggccggg ctgggggcgc ccgggatcga agcagactgt gggcgggtgc
    16381 ttgagctact caaaagacgg ccgtgcaccg tcagcgacat agccgaaggg ctcgatctca
    16441 acatcaatga ggccgctaag tttgtgggga ttctcaaggg agaaggactt atctgcgcca
    16501 tccgccgaga aggacgggcc tattttttcg ccaacgatgg gaactgaacc attgccggca
    16561 catttctgcg ctcagattat tgacatatgc tcacaaccat gggtatactg gtaatgaaca
    16621 tatgcccata accctacaac agcaattgat ggcgtcaagc caagcacgta ttcaatccaa
    16681 ccggaggatg ggcatatgaa aaagccgtgg aggtagtcgt tcgatcaaac cccaaggctt
    16741 tggagaggag gtgctttaca tgccgagagg agatggaaga ggaccaactg gtttcggtcc
    16801 tatgacaggc aggggcgcag gcttctgcac cggatatgat gttcctggat atgctaatcc
    16861 cgcaatgggc agggggccgg gatttggacg cgctccgcgc tgttatgggg gaggcggatt
    16921 cggacgaggg tacagaagga tgtactacgc gacgggactg ccgagatgga tgcgttctgg
    16981 agcaggggca tggggtcagg ccgcgtacgg gcctgcttac agcccttacg cccaagaggc
    17041 catgaggccg tcgaaagaac aggagatagg ctatctaaaa cgacagtcag agtttctcat
    17101 ggaggagctc gaggccgtcc gggggcgtct gagcgagctt tctggttccc aagagtacga
    17161 tgaaggctag tccttcgagg cgaaggggtg agagaggtcg gatttgagcg tccttcggct
    17221 ccttcgccgg ccagcaatcg acctgtaccg aggatagtgg acaaagttgc cggaggttaa
    17281 ggggagatgg tagtttgaag ctagcagtaa cggcgcaggg aaccagcctc tcagccgggt
    17341 tggacccgcg tttcggcagg tgcgaacatt ttgtgatcat agacgtcgat accgaggaat
    17401 tcgaagcatt ctccaacgag agcgttatgg cgtcaggcgg agcgggaact gaaagcgccc
    17461 agtttcttgc tgacaagggc gcagaggcgg tcgttaccgg aaatgttggt cccaatgcag
    17521 cgatggctct ggaagccgcg ggcataaagg tctacagaat gtcttccggg acagtgaagg
    17581 acgctttgga ggcttttaag tcagggacgc tcgaggcaat ttccggacct acagtcaatt
    17641 cccatcatgg aatgggcggg catggccgcg ggggtcgcta ggctcaaggg ctgggccagc
    17701 tggcaagtgg ggcagacccc gtcggcgcaa acggagcttc ccacgatagg atcgagcaga
    17761 attggtcttt tcacatttaa gggggtaacc atagatgaag attgctgtgg caactgacgg
    17821 ttccaccgtc tcgcctcatt tcgggcattg ctctgaattc accatagtca cagtggaaaa
    17881 cggcaaggcc gtcgattcgg ccattcttcc aaaccccgag catcaacccg attttcttcc
    17941 tcagtacctt tctgaacacg gagtttcctg catcatcgca ggcggaatgg gtccaagggc
    18001 ccagcaacac tttgctgcta ggggcatcaa gaccgtgata ggagcgtccg ggagtgtgga
    18061 cgatgtcgtc cacgcgtatc ttgcgggatc tcttgaggtg ggcgagagcg cttgcgatca
    18121 cggccacgga catgagcagc acggtgaatg cggacacggg gaaaacgggg gaacccatag
    18181 gcgcggctgc ggccacaact agcgggggat tggcgggcag gcccgttcga gcctgcctgt
    18241 ttttggaagg accaaatacg cgaagttggg gcatgcacga tgaatggtgg cgatggaatg
    18301 tgattgtttc aatagcgagc ggcaaagggg gaacgggcaa gactactgtc gcggtcaact
    18361 tggcgttggc cttggaaggg gctatgccga tacgcttttt ggactgcgat gttgaggagc
    18421 ccaatgccca tcttttcctc catcccgaaa tcgagcgctc cgaaaaagta agtttgccag
    18481 ttccggttgt ggacgagagc aagtgtaacg gatgcggaaa atgcgcccgg gtgtgcgctt
    18541 tccatgcgat ccttgccttc agctcccgga cgatcgtcct gcccgagctc tgtcatggat
    18601 gcggggggtg tgcgcttctt tgcccgcagg gggcgataac ggagagcccg aaggtgatcg
    18661 gggaagtgga gacggggcgc gcaggagata tcaggtttgc ccatggacgg acagggatcg
    18721 gaagccctct ggcaccgcct gtggtgaaag cggtgaggga ggcgttggac aaggaaggcc
    18781 taggggccgg gttgacgatc atagacgcgc cccccgggac gtcctgcccg gttgtggctt
    18841 cgatcaaggg cagcgactac tgcgtgctgg tgacggagcc tacgcccttc ggcctcaacg
    18901 atcttgggct ggccgttgga ctggttcggg agctaggcat accctgcggc gtagtgatta
    18961 acagggccgg gttgggggat gaggccgttt cagcctattg cagccgcgag ggcatccctg
    19021 tgttgcttga aataccatac gacaggaagt acgccgaatg ctacgctaag ggcggaaggc
    19081 ttgtggatga ctttccagag ctccgaggcg ctttgaaggg gttgtggcgc aagatcagtg
    19141 cgagcggcga atctggcgca gagggagagg atgggcgaag ggagatgggt gagatatgaa
    19201 ggagttgctc gtgataagcg gcaagggagg caccggcaag acgagtgtag ttgcgtcatt
    19261 tgcggctttg gcggagaaca aagtcttagt cgactgcgat gtggatgctg ccgaccttca
    19321 cctcttgctt tcgccagagg tagttgaatc ccatgaattt atggcttcgc gacaggcaac
    19381 tgtcgatgtg agccgatgca cggggtgcgg tcattgcagc gaagtgtgcc gcttcggcgc
    19441 tatagccatg gcctatgggc atccaaccat cgatccgatt tcctgtgaag ggtgtactgt
    19501 gtgctcccat atttgtcccg agggtgctat aaccatggtc gacgtcatgt cagggcactg
    19561 gtacgtatcc catacccggt ttgggccgat ggtccatgcc aagctcaagg cggctgaaga
    19621 gaactcaggg aagctcgtat cccaagtgag gcgcaaggcg cgggagatag ccgaaagcca
    19681 ggggcacgag ttgatcatta ccgacgggcc gcctggggtg ggatgccccg tcatatcctc
    19741 catctccgga gtggatcttg ccttgatcgt aacggaaccc acggtggcgg gggtccacga
    19801 catggagcgg gtcctcgacc ttacgtgcca tttcggtgta gagtcggcgg tatgtatcaa
    19861 caagtgcgat cttgatgagg ctaatgctga gcgcatagag gattactgtt gtgcaagggg
    19921 aatcgagatt gcggggaaga tcccgttcga tgaggatatg gtaagggcgg ttgtaagggg
    19981 tgagccgcct gttctgtacg gagatggtgc tcctgattgc gcgggcccag atagcgttgc
    20041 gggcgaagcc gcgcaggcta tacgccagct ttgggatagg gtcgctgttc ttctcgcgcg
    20101 tgatgcg
//
LOCUS       DAOSYL010000045        19171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2362_length_19171_cov_8.307282, whole genome shotgun sequence.
ACCESSION   DAOSYL010000045 DAOSYL010000000
VERSION     DAOSYL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19171
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_19171_cov_8.307282"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..195
                     /locus_tag="PLU15_07835"
     tRNA            108..195
                     /locus_tag="PLU15_07835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:142..144,aa:Leu,seq:cag)
     gene            complement(454..1047)
                     /locus_tag="PLU15_07840"
     CDS             complement(454..1047)
                     /locus_tag="PLU15_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30872.1"
                     /translation="MEEEKTVNWFARLIGIITAPPVMAGAAAATVRHYDPTLFGDGTT
                     WYLVTLMLLTAIPLFSYVVAYMVPSIRAMGRKGQRKLAFIVSVICYVVGAAVCLIAKA
                     PRIVSAFFLSYLASGGLLSLINAAFKFKASGHACGFAGPVTLVSMVIGPVALWAFALL
                     PLVFWARLSMKRHSKGELWSGALVGIGATAVVMLIFP"
     regulatory      1797..1940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2053..3600
                     /gene="pruA"
                     /locus_tag="PLU15_07845"
     CDS             2053..3600
                     /gene="pruA"
                     /locus_tag="PLU15_07845"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOO30873.1"
                     /translation="MPLKQFSNEPLMDFSIVENRDRMADALATVEEEFGHYYPLIIAG
                     QRVETEERVASVNPCAHEQVVGYVAQADRAQAEMAMEEAWKAFETWREVPAEGRAATV
                     LRLSALMRKRKAELAAWLVFEVGKNWVEADADVAEAIDFCEYYAREMIRYEGLNQVYP
                     HPNEQNVQRYIPLGVGVVISPWNFPLAILTGMTTAAVVAGNTVIIKPANTAGVIAAKL
                     MELVDEAGFPPGVINYLPSSGKTIGDYLVQHPKTRFINFTGSKDVGLHINEVAAKHQP
                     GQIWIKRVVAEMGGKDFIIVDETADLDKAAMGIVKSAFGYQGQKCSACSRAIIVDSVY
                     DEVLEKTREMAAELRVGEAAANFAVNAVIDEKAFRSISNYIDIGREEGRIVLGGDRDS
                     SVGYYIEPTIIEGVSSHARVAQEEIFGPVLAFIRADSYDHAVEIANCTEYGLTGSVYS
                     RNRARLESAKDKVHVGNLYLNRHCTGALVGLHPFGGFNLSGTDSKAGGPDYLLLFMQA
                     KAISEAM"
     gene            complement(3716..4222)
                     /locus_tag="PLU15_07850"
     CDS             complement(3716..4222)
                     /locus_tag="PLU15_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOO30874.1"
                     /translation="MMIDYYAILGVSQHAGEELIRAAFREKARLWHPDVCHSPEAHER
                     FLVISRAYSTLIDPTRRAQYDRDLARADKTRIRSGFSCSASTSARFSHKLLVGLAGVL
                     LIIASALAIAGTAIPLALPPLIPLLVWLLHQSKLIGLQVVVTYTILASCLLLGLLLLG
                     LGLAYLSA"
     gene            complement(4247..4765)
                     /locus_tag="PLU15_07855"
     CDS             complement(4247..4765)
                     /locus_tag="PLU15_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30875.1"
                     /translation="MIAGEPLDVVRAFEDAYNDHDAKTINSLYAPDAKFQVENIMAIE
                     GKPALQGKTDYDIELHTHIHINPVSVKENRLRARVMASSEWLLETGIGTAQYEAEFEV
                     ADGMIKSIIARPVPQTKAAIGRVLNDFIEWAMTERPCEAGILMPDGRIVFNAHNARLG
                     LIMLKEWKAKRD"
     gene            5375..6154
                     /locus_tag="PLU15_07860"
     CDS             5375..6154
                     /locus_tag="PLU15_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30876.1"
                     /translation="MINALGLTKRFGDTLAVDSLDLHVGAGEIFGFLGPNGAGKTTSI
                     NMMIGILTPTAGRVELAGIDVAKDPLKAKAIIGYVPDQPNIYEKLTAWEFLMFVANLH
                     EVERGRAEKKARNLLKMFDLLDRADEQLGGYSHGMKQKVVIASALLHEPKILFMDEPT
                     VGLDPGSARLVKDILRELASEGVTVFMSTHVLEIAERMCDRVGIIQKGRLLAVGNMDD
                     LREVAAAKGAMKHPEASTLEDLFIELTGGSDYAEVSRYLEG"
     gene            6160..7953
                     /locus_tag="PLU15_07865"
     CDS             6160..7953
                     /locus_tag="PLU15_07865"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30877.1"
                     /translation="MDKTFLILSVELRQAFRGTRGESEKSSQGKRIARNALGYLALIS
                     LAIFVGTSVYNIAAALSDTLAAYPDMVRAVWLNLMSGLSLGVFVMLFMTGISVVYQAL
                     YDSGDLKFLLSTPTPVGSVVGAKLTVALVRNLAALVPFMYPIWVGYGAGSRAPFSFYF
                     MSILAILLAALLFTAVTAIFAMIVMRNVPTQKMRQVIMIGSLVVGFMFVLATQVLSAK
                     MTRSNGLDATALAQAAQGWGLGRTWYLPHVWVVKTAFLTTPGFGFSLWESLFPLAVAA
                     IGAFVGAIGLSANAFVTGWSAAGDVETGKSGAMRRRRTRKGTGEKAICCELADDAESE
                     MPDTMIRRHTGQGWGIFAKDLTIIKRQPIIWYSILVAIVASGFYLYNMSYGARSGDVG
                     AIPDIMKDVILFVLVMMSTISSGQIAGMCISMDGESLWLLRSMPIDPGTYFNAKMALA
                     VMPGATVFTVLALAMAFIPDIPQYPLYLSLPVGYAAISMLGALEVMSDAMRPNFSFRM
                     SGASGTQNQKDPGKSLISLGISMAGTFVLGAAFAFNNYYSHLGFSEGMSRQAAILVGT
                     IAFATIVVAGHAIARSLALASIKRILRGESN"
     gene            8076..8222
                     /locus_tag="PLU15_07870"
     CDS             8076..8222
                     /locus_tag="PLU15_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30878.1"
                     /translation="MDLSKSFDCVSHDIVMAKVARVVEDRLVLKLIREFFEAKVMEDD
                     VCKN"
     gene            complement(8746..9501)
                     /locus_tag="PLU15_07875"
     CDS             complement(8746..9501)
                     /locus_tag="PLU15_07875"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO30879.1"
                     /translation="MLGYSYLRGAEKVLDEIATTQMGAIEKAASAIANSLSHGGLLYL
                     FGTGHSASVAVEPFHRAGSLVPVRPIVEHAMTLHSSPKKATALERLEGYAGIILDDSG
                     IRPGDSLIVISNSGINSVPVEMALGAKSRGVTVIAITSVRHSKAESSRHPSGKRLFEA
                     ADIVVDNCSIPGDAMLDTCASPIKIGPTSTLAGIAIVNAISVKVIGKLHEMGIEPPVM
                     MSSNIAGGDDFGAKWDEAYADSEAARSLASKRP"
     gene            10073..11506
                     /locus_tag="PLU15_07880"
     CDS             10073..11506
                     /locus_tag="PLU15_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO30880.1"
                     /translation="MISTLDFAKPLVNQALGYLQADPVGNLPKALAIAEKLASQENHK
                     QQVREVGKAILDPDSNWRELAIRLLTETHPNIMKGIGVNFFVNSALVGVPTQYDLAEE
                     LGVQVPYAILMDPTEKCNLRCTGCWAGDYQRVRELPFETMDRVCTEAEELGIYLIVVS
                     GGEPMVAKDKLIRLAEKHPNQLFHPFTNGTLFDEEFVSEMIRLGNIAPAISVEGLEEQ
                     TDARRGKGVYQKIMNTMDMLREAGCVFGFSACYARNNTEIIASDEFIDTMIDKGCSFG
                     WFFTYVPVGSDPNLDLMATPDQRALMYDTVRQWRFTKPIFMVDFWNDGEFALGCIAGG
                     RRYLHINANGDVEPCAFIHYAVDNINEKPLKECLASPLMKAYQKRQPFNDNMLRPCPL
                     IDNPEKLVEIVEESGAHSTQLGEHLVDHRELAEMIQEGYTKHWAPKADEIWNANPHPF
                     FEMQNKYYEEERTEDRKKQKEIQSKQG"
     gene            complement(11856..12968)
                     /gene="mnmA"
                     /locus_tag="PLU15_07885"
     CDS             complement(11856..12968)
                     /gene="mnmA"
                     /locus_tag="PLU15_07885"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOO30881.1"
                     /translation="MGPNINNQTIAVAMSGGVDSSVAAALLVAQGHRVIGITMTTWHW
                     PGPFEGDACGDSFGEEAADSAARVARSLGIDHVVVDLSEEFKKTVVADFVRAYAGGST
                     PNPCVICNQLIKFDLLHHKARELGADMVATGHYARLRQDAVSGRYLLLRPADSRKDQT
                     YMLYRLGQEQLARSVFPLGDLEKVQVRKIAKSIGLEAADRPESQEICFLPPGKYREFL
                     ASNDPSFQTPGPIVDTEGHVIGEHRGIAMYTVGQRKGLSLAADRPLYVLEIRPDSNTI
                     IVGPVERLYSSWLEVESTNFIPFDTLSDKMDVQAKIRYRSPAAKAVIFPAANGRVRVE
                     FNEPQRAITPGQSAVFYDGDLLVGGGIISSNQKADV"
     gene            13286..14503
                     /locus_tag="PLU15_07890"
     CDS             13286..14503
                     /locus_tag="PLU15_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOO30882.1"
                     /translation="MYTARISISGHLIDSGLLSRLLDAVSDLGGKFEIVETQFGRMKE
                     EESCATIEVGADTCEELDKILDKAKELGATVFDGEPVQLAVVEQDGVCPDEFYTSTNI
                     ETYVNIDGHWVPVDDIEMDCAIVVDAQKPAARCCPLAAVSRGEYVVVGSSGIRVASMA
                     SAKETEVFSFMGSDVSSERPKALVVEQIAREMKEVRARGGKILVVAGPAVIHTGAGPH
                     LARLIDAGYVNVFYAGNAVATHDVEWALLGTSLGVYLKDGTSAPMGHTHHLRAINTIR
                     KAGGLRPAVEKGILTKGVMYSLVTNDVPYILAGSIRDDGPLPDVITDSVKAQDAMRKN
                     LKGVEIALMLSTMLHAIATGNLLPARVKTVCVDINPATVTKLADRGTFQAIGIVSDVE
                     WFLKELAGHLAAN"
     gene            14638..15252
                     /gene="pncA"
                     /locus_tag="PLU15_07895"
     CDS             14638..15252
                     /gene="pncA"
                     /locus_tag="PLU15_07895"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOO30883.1"
                     /translation="MEALLLVDLQNDFLPGGALGVLEGDQVIPVANRVQEYFDIVIAT
                     ADWHPPGHGSFASSHPGCKAGDVIELSGMPQVLWPDHCVQHTEGAEFSPHLNTGKIDK
                     VIYKGMDPEIDSYSGFFDNGRQKATGLETYLRDQKVDTVYVMGLATDYCVKYTALDAV
                     SLGYRTWLIEDGCRGVGVAEGDVERALAEMKAAGVRVVKSGDLG"
     gene            15343..16518
                     /locus_tag="PLU15_07900"
     CDS             15343..16518
                     /locus_tag="PLU15_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOO30884.1"
                     /translation="MKFQGLVIGVPTEIMKGERRVAAMPDTVKKMTAEGAKVLVQKGA
                     GEGSYFHDEEYSAAGAEIVDNVEDLFARADIILKVKEPIFNDAVGKHEVDMMHEGQCI
                     ITFLHPAAPANHQMVQDMAAKGVISLTLDGIPRISRAQTMDALTSMSTVAGYKACLMA
                     ANRLPKFMPMVGTAVGMIKPSTVTVIGTGIAGLQAVATAKRLGAVVNSIDIRPDANEQ
                     AKSLGAKVVDVGVPSEIAIAPGGYAAKLPDEWLEKERETVKPVVIDSDIVITAALIPG
                     KLAPVIVTEEMVKAMRPGSAIVDIAIDQGGNVEITQPGEVIEKYGVSIDGTKNIPGMM
                     PTASTWMFANNIYNYTANLVKDGKIDLNLDDEIIKSSLVTRDGQIVHEGAREAMNMK"
     gene            16534..16818
                     /locus_tag="PLU15_07905"
     CDS             16534..16818
                     /locus_tag="PLU15_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOO30885.1"
                     /translation="MSELSLIILFAIATFVGYKVISQVPSLLHTPLMSGTNALSGVTV
                     LGALSATAAAKAQGASAVGAVLGCVAIIMAMINVVGGFLVTDRMLRMFKK"
     gene            16854..18851
                     /locus_tag="PLU15_07910"
     CDS             16854..18851
                     /locus_tag="PLU15_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOO30886.1"
                     /translation="MDWNEAIFLGLSLADLLGLVFVAGTVWGIRLMSSPATAVRGNLI
                     GAVSMTGAIIVTLVAEDIVSVQILWLGMAVGGALGYILAARVAMIEMPQMVALLNGFG
                     GGSSAIVSLLTLLGPGAIGPFEKVTAILGLLVGAITLSGSLIAAGKLAGKINQRPVIF
                     ENQTFVNAVAIIISVAAGVRAVLAPADAVLWPSVIALVASLAYGVLFTIRVGGADMPI
                     TVSLLNSLSGVAASVSGLAIRNPLLVAIGAVVGASGLILTQIMCRAMNRSLGQVLTGK
                     TTVAHIKGSEAKPVSAPTKAAAGETSAQARLASKAEKPEKADYISIAKAAKKVIVVPG
                     YGMALAQAQGDVKALMDKLEAGGADVKVAIHPVAGRMPGHMNVLLAEVDVPYDKLYEM
                     DQINDEFAETDLVIVVGANDVINPAANTAEGTPIYGMPILNVYQAKHTVIFNYDTKPG
                     YAGVDNPLYEADNVTLVLGDAASTVKEFMAELDASGAAAESQLSPGKVDCLDIARAAK
                     KVIVVPGYGMAIAQAQRDVKALMDKLEADGADVKVAIHPVAGRMPGHMNVLLAEADVS
                     YDKLYEMDQINDEFAETDLVIVVGANDVINPAANTAEGTPIYGMPILNVYQAKHTVIF
                     NYDTKPGYAGVDNPLYEADNVTLVLGDAASTVKEFMAKLGL"
BASE COUNT         4436 a         4661 c         5557 g         4517 t
ORIGIN      
        1 ggtaactatt gcatactggc gcgtggcgtg atatactagc tgagcaggct gggatgtgcc
       61 tttttcacag ggcgcatcca aagcgacaag tgaacatgtt tgatcgtgcc gaagtggtgg
      121 aattggcaga cgcgctagat tcagggtcta gtgggggtta accccgtgcg ggttcaagtc
      181 ccgccttcgg caccattatt ttgggcagga ttgtttcctg cccattcatt ttgtttcaca
      241 tctgagggcc catgctctga gcctggcgca agaaatcggt tccgatcgct ggcgccaggc
      301 atcgtaattt ctgctttcgc agcactgaag tgctgagaca gcttcagatg gcctgcggta
      361 tctggcaggc caaattggca agcttcggca gcgcagactg acagaccacg cgaaatcggc
      421 cgctacccgc tgacaagctg gccacgccac cgcctacggg aagatgagca tgactacggc
      481 agttgcccca atccccacta atgcgcctga ccaaagctcg cctttggaat gccgcttcat
      541 agaaagcctg gcccagaata cgagcggcag aagggcaaag gcccaaaggg caacagggcc
      601 tatgaccatt gacacgagag tcacagggcc ggcgaagccg catgcatggc cgcttgcttt
      661 aaacttgaag gcggcgttta tcaggcttaa taagcctcct gatgcaagat atgatagaaa
      721 gaatgcagac acaatccgcg gagccttggc tatcaggcaa actgcggcgc cgactacgta
      781 gcatatgacg gaaactataa atgccaattt tctctgacct tttctgccca tcgctctgat
      841 gctcggcacc atgtacgcta ctacatatga aaacaaggga atcgcggtga ggagcatcaa
      901 tgtcaccaga taccacgtcg tgccatcccc aaacagcgta ggatcgtagt gacgaaccgt
      961 tgctgcagcg gcgcccgcca ttacgggggg agcggtaatg atgcctatca atcgggcaaa
     1021 ccagttcact gtcttttctt cttccataag ctgtcctccg ttcttcagcg cttaccgagt
     1081 aagcctctat tggatagtgc gcggctaggg agtttgggtt gcttgaagtt caggcctgac
     1141 ccgcccggtg aggatgggat gcttctttgg aactgttcac gttgcacggt acagatgcga
     1201 tggcggaacg aatatcggtc ggaagcgagc agcgcgagca cttaacttta ataatattct
     1261 tcactggaca ggaggcttcc tgcctcgaaa gctggaagcc caatacttgc agaccatttc
     1321 aatgcggttc tcgattgcgt tgtcctatct gcctgaccgg tgcgtataac cctgcggtag
     1381 ctgcttgata tacatagaac agggagacag gcttctttga cggttggcgc agccccgaag
     1441 gttcttcatt cgattgacga ttccgcaggt cattcatcac agccattacc gatggagtct
     1501 tagccgccta cataaccctt ctaggtgctt ttcggatatc ggggcacgaa atctggccgc
     1561 tcacgaatgc tctgacatat cgtgaatagc attatgtcaa gaactcgcgg ccttgcgagg
     1621 tagatgcgct gatggctggg cagtcttgat gtagtaggtt ctgtgaaggc tggaaaggtc
     1681 aatatgtatt cgggcacgcg cagtgaagtt ggctgcaggg tgcgcgagtt cggcttactt
     1741 gcaatatatt gtaagttgag tgcaagttgt aggattcctg ccatttttgt cgaatctgcc
     1801 atatagcgcc tccgagtcat ctcttcttac ccgatgatgc tggagcggac atgaccggtg
     1861 gcaactacgt tgttgcctga gggtctggct ggaaacggcc tggcctcccg tgtttggaaa
     1921 ggagcgcagg caggctccgt cacatgcgcg ttcggtggcg gacagggcat gccatgcgct
     1981 tgcaagacac gaaacgtctc tttgttcact gtgttgtgat gctgaccctc ggcaacgtta
     2041 ggaaaggggt ctattccgtt gaagcagttt tccaatgaac cattgatgga cttctcgatt
     2101 gtggaaaatc gagacaggat ggctgatgcc ctggcgactg tggaagagga attcggacac
     2161 tattatcccc ttataatcgc aggtcaacgc gtggaaaccg aagaaagagt cgcgtcggtc
     2221 aatccttgcg cacacgagca ggtagtgggc tatgtcgccc aagctgaccg ggcccaggcc
     2281 gaaatggcta tggaggaggc ttggaaggct ttcgagacct ggcgtgaggt tcccgctgaa
     2341 ggacgcgctg ctacagtcct gaggctgtct gcgctgatgc gcaagcgcaa agcggaactc
     2401 gctgcctggc tcgttttcga agtgggcaag aattgggtgg aggcggatgc agatgtagct
     2461 gaggccatcg atttctgcga gtactacgct cgggagatga tacggtatga aggcctcaat
     2521 caagtgtatc cacaccccaa cgagcagaat gtgcagcgtt atatccccct cggagtaggg
     2581 gtggtgatca gcccgtggaa tttcccgttg gcgatcctca ctgggatgac gactgccgcg
     2641 gtagtggccg gaaacactgt gatcataaag cccgcaaata ctgctggtgt gatagcggcc
     2701 aagttgatgg agctcgtgga tgaggcaggt tttcctccag gcgtcatcaa ctacctgccc
     2761 agcagcggca aaaccatagg tgactaccta gtccagcatc ctaagacaag gtttatcaac
     2821 tttaccggat cgaaagatgt gggccttcac attaacgaag tggccgccaa acatcagcca
     2881 ggtcagatat ggatcaagcg cgtagtggca gagatgggcg gtaaggactt catcatcgtg
     2941 gatgagacgg ctgatttgga caaagcggct atggggatag tcaagtcggc cttcggatac
     3001 caagggcaaa agtgttctgc ttgttcaaga gcgataatcg tggactcggt ttacgacgaa
     3061 gttctcgaga agacgagaga gatggctgcc gaattgagag tgggggaagc tgcagcaaac
     3121 ttcgccgtta atgcggttat cgatgagaaa gctttcagga gcatttccaa ctatatcgat
     3181 attggccgtg aagaaggaag aattgtgctg ggcggcgatc gtgattccag tgtcgggtat
     3241 tacatagagc ccaccatcat agagggtgtg tcttcccatg cacgcgtggc ccaggaggag
     3301 attttcgggc ctgtgctcgc atttatccgg gcggacagct atgaccatgc ggtggagatc
     3361 gccaattgca cggaatatgg gctcacgggt tccgtctaca gcaggaatag ggcgcgattg
     3421 gagtctgcca aggacaaggt tcatgtggga aatttatatc ttaacaggca ttgcactggg
     3481 gcattggttg ggctccatcc gtttggcggt ttcaacctga gcgggaccga cagcaaggcg
     3541 gggggccccg actacttgct ccttttcatg caggctaaag cgatttcaga ggctatgtaa
     3601 ggtctaaagc tcgcaaaaga tcggagttta tctccgccct ccgccatgat acaatggccg
     3661 agccttaaga tggcaaagag catctttgtg ggctcggcct cgtcctttta cgcgttcatg
     3721 ccgaaagata ggcgaggcct aaccccagca gtaagaggcc gagaagcaga caactggcga
     3781 gaatggtgta tgtgacgacg acttgtaacc cgatcagctt cgactggtgc agaagccata
     3841 caagaagggg tatcagcggt ggcagggcaa gggggatcgc ggtccctgca attgcgaggg
     3901 ctgaggcgat tatcaacagc acgcctgcga gtcctaccaa aagcttgtga ctaaacctgg
     3961 cagatgtgga cgcagaacat gagaagcccg accgaatccg cgttttatca gcgcgcgcaa
     4021 gatccctatc gtactgggcg cgacgggtgg ggtcgataag tgtgctgtat gcccgggaaa
     4081 taactaaaaa gcgttcgtgc gcctcagggc tgtggcacac gtcgggatgc cacagcctag
     4141 ctttctcgcg aaaggccgcc cttatcagtt cttcaccggc gtgttgagaa acgccgagaa
     4201 tagcgtaata gtcgatcatc atgcaccacc atcatgcgtg tggggctcaa tcgcgctttg
     4261 ccttccattc tttgagcatg atcagcccca gtctggcatt gtgcgcattg aacacgattc
     4321 tgccatctgg cattagaatg cccgcctcac agggccgctc cgtcattgcc cactcgatga
     4381 agtcgttcag aacgcgcccg attgcggcct tagtctgagg aacgggtctg gctatgatcg
     4441 acttgatcat accgtcagcc acctcgaact ccgcctcata ctgggctgtc ccgattcctg
     4501 tttcgaggag ccattcacta gacgccatta ctcgggcgcg cagcctgttt tcctttacag
     4561 agacaggatt aatgtgtatg tgagtatgca gctcgatatc gtaatcagtc tttccttgaa
     4621 gggcgggttt gccctctatg gccatgatgt tttcgacttg gaattttgcg tccggggcgt
     4681 atagagagtt tatggttttc gcatcatgat cgttgtaggc gtcctcaaat gctcggacaa
     4741 cgtcgagagg ttcaccggca atcacattcc aacacctcgc tcatcttgat cagattgtcg
     4801 aacgtcgcta gcgcaggatt ttctgagcgc gcgatggatc atggatgata gcctagagga
     4861 ttgaagatgg gcttgatcct ccttcacgtg tatctcctga ggatttgtca gacagccggg
     4921 ttgcatattg cctattctag attcgcccac gtacacctgc aaactattcc atcaaggcgt
     4981 ttgcctgcca cttagctgcg tccatcataa gcgaatactt ccggtcttgc cgcttctgtg
     5041 gtgttggaac gttcaatttg ggagatggct gcacggatct tgaatggtag gtgcgtcgag
     5101 gcgtaccgca tgtcggggcc gaatatgact ttaaagctcc caaatatcat cttaccatgt
     5161 gccgtgctag agaaggtgat gtataattgt atgagggtgg ctgttccctc atgcttcgcg
     5221 tcgtcaaggc agaagacggg tttgagatgg caaaaaagaa cgcagacggg gccatgaaga
     5281 tctattcgaa ttctagatct tgacgacctc tgatagggat atgccttttg gcactgtgat
     5341 tttgaaggtg gctgcgaaca ggagggattc tttgatgatc aatgcactcg ggcttaccaa
     5401 gaggttcggc gacacgcttg ccgttgacag ccttgatctt catgtaggcg caggcgagat
     5461 attcggcttt ctcggtccca atggagccgg taagaccact tctatcaata tgatgatagg
     5521 gattctcact cctaccgcgg gtcgcgtcga gctcgcaggg atagatgtgg caaaggatcc
     5581 tctcaaggcc aaggcgatta ttggttacgt gccagaccag cccaatatct acgagaaact
     5641 tacagcctgg gagttcttga tgttcgtggc caatctccac gaagttgaga gaggacgggc
     5701 cgaaaaaaag gctaggaacc ttttgaagat gtttgatctt ctggatcggg ctgatgagca
     5761 actgggtgga tactcgcatg ggatgaagca aaaggtcgtc atagccagcg cgctcttgca
     5821 tgagcctaag atactgttta tggacgaacc tactgtagga cttgatccag ggagcgctag
     5881 gctggtaaag gatatcctcc gcgagcttgc ttcggagggt gtcacggttt tcatgagcac
     5941 acatgtacta gagatcgcag agcggatgtg cgatcgggtg ggtattattc aaaaggggag
     6001 attgctggct gtgggcaaca tggacgatct acgtgaggtc gcggcggcca aaggcgccat
     6061 gaagcatccg gaggcttcca cgttggaaga ccttttcata gaactaactg gcggttccga
     6121 ttatgcagaa gtctccagat acttggaggg ctagacgaca tggacaagac gtttctcatc
     6181 ctatcagttg aacttcgaca agcgtttcga ggaacccggg gtgagagtga aaaatcgagt
     6241 cagggcaagc gcatagcgag aaatgccctg ggctatttgg ctcttatcag tttggccatt
     6301 ttcgtaggta ccagtgtgta caatatcgct gcggccttgt ccgatacgct tgccgcttat
     6361 ccagacatgg tgcgggcagt atggttgaac ctgatgtcag gtttatcact aggagttttc
     6421 gtcatgctct tcatgacagg tatctccgtg gtctatcaag ctttgtatga ttcgggcgat
     6481 ctcaaattcc tgttgagcac ccccacacct gtgggttcag tggttggggc gaagttgacg
     6541 gtggcccttg tgaggaacct ggcggcgctt gtcccattca tgtacccgat atgggttgga
     6601 tatggtgccg gtagtcgcgc accgttctct ttctacttca tgtccatcct ggcgatcctc
     6661 ctcgctgcac tgctgtttac cgcggtgacg gccattttcg caatgatagt aatgaggaac
     6721 gttccaactc agaagatgag gcaagtcatc atgatcggct cgcttgttgt gggatttatg
     6781 ttcgtcctcg ccacgcaagt tctcagcgcc aagatgaccc gttccaatgg cctcgatgcc
     6841 acagccttgg cgcaggcggc ccagggctgg ggactgggca ggacatggta tttgccacat
     6901 gtctgggtgg ttaaaacggc ctttctcact acgccgggat ttggattttc actctgggaa
     6961 tcgctttttc ctctggctgt tgctgcgata ggggcatttg taggcgccat aggcttgtcg
     7021 gccaatgctt ttgtgactgg ttggtcagct gcaggcgatg tggagactgg caagtcaggc
     7081 gccatgcgtc gtcgacgaac gcgtaaaggg accggagaga aagcaatctg ttgtgagctg
     7141 gctgacgacg ccgaatcaga aatgcccgat acgatgattc gaagacatac cgggcagggt
     7201 tgggggatct ttgccaagga cttgaccatc ataaagagac agcccattat atggtatagc
     7261 attcttgtag ccatagtagc atctggattt tacctctata acatgtccta cggggcgcgt
     7321 tcgggcgatg tgggagccat tcccgatata atgaaggacg tgattctttt tgtcttagtc
     7381 atgatgtcga cgatttcttc agggcagata gccggcatgt gcatcagtat ggatggggag
     7441 agcctctggc tgcttcgatc catgcccatc gaccctggga cgtacttcaa cgctaaaatg
     7501 gcgctggcgg tcatgcccgg ggccacagtc ttcaccgtgc tcgcgctggc tatggctttt
     7561 atccccgaca ttccccaata tccgctctac ctgtcgcttc ctgtgggata cgccgccata
     7621 tccatgcttg gcgcgctgga ggtcatgtct gatgccatga ggcccaattt cagcttcaga
     7681 atgtcggggg catcagggac gcaaaaccag aaagaccctg gcaagtcgct catatccttg
     7741 gggatcagta tggcagggac attcgtcttg ggtgcggctt ttgcattcaa taactactat
     7801 tcgcatctcg gcttttctga gggtatgagt agacaagcgg caatccttgt tggcacgatc
     7861 gcttttgcaa ctatagtcgt cgcaggacat gcgattgcgc gaagtctggc tttagccagc
     7921 atcaagcgta tattgagagg cgaatcaaac tgagcaaaaa ccgctttgtg ctgggcacag
     7981 gcgtacgttg tccccatcgg tttcatggga tgctgtaagc gatctagaag gtcgtaaaac
     8041 agagggggaa ggtggggacc gattggggat ttcctatgga cctatccaaa tccttcgact
     8101 gcgtcagtca tgatatcgtc atggccaagg tggctagggt ggtcgaagat aggctagtgc
     8161 tcaagcttat ccgggagttt ttcgaggcta aagtcatgga agacgacgtg tgtaagaact
     8221 gagtagggcg aggcacaagt cggtccgagg agtcctctgt tcgccaacat catgctgaac
     8281 gacctaggat gagaactcga gaagagaggg catagattca ctaaaagtcg gcgcgccgga
     8341 gaacgggtga tggcgagcgt tcgacgtttt gtggagggga aatctgaagt tcaagctcca
     8401 attgtggaaa tcaggccgca accacgaccc gggagcggtc taaagagcgg atttggaggc
     8461 tcatcggcct cagcgagagg tcgagtatcg acagaggcct ttgcgagctc agtctctacc
     8521 tgtgtgggtg gacgggatac tgccggatcg cgcggacacc tagggtatac cagaggctag
     8581 actaatggat gcagcgatgc gcgcggctcg gcaaggtcac ccaaagccga agtcttctcg
     8641 gacaccggta ttttgcaagg caacgcgggt ttgaatactg gggtgttgcc gcgttctttc
     8701 ctactgtgga taggctttta gcgaagagga agcgcaactt ggcgcttagg gccgtttgct
     8761 cgcaagcgac cttgctgctt cggagtctgc ataggcctca tcccattttg ctccgaaatc
     8821 gtccccgcct gctatgttcg agctcatcat cacaggcggt tcaataccca tttcatgcag
     8881 cttgccaatg accttgacag atattgcgtt gacaatcgcg atcccagcta atgttgatgt
     8941 cgggccgatc tttatcgggg atgcgcacgt atcgagcatt gcatctcctg gaatgctgca
     9001 attgtccacc acgatgtctg ctgcctcaaa caacctcttg ccgctaggat ggcgggaaga
     9061 ctcggcttta ctgtgtctta ctgacgtgat agctattacg gtgacccccc gagacttggc
     9121 gccaagcgcc atttcgaccg gaaccgagtt gatgccggaa ttggatatga caataagcga
     9181 atcgcctggg cggatgcccg agtcgtccag tatgatgccg gcatatccct ccaggcgttc
     9241 cagggcggtg gcctttttag gggagctgtg caacgtcatg gcgtgttcga ctatgggtct
     9301 gacaggcacc aaactgcctg cccggtggaa cggttctacg gcgactgacg ctgaatgtcc
     9361 ggtgccaaaa agatatagca ggcctccgtg gctcagcgaa tttgcaattg cggaagccgc
     9421 cttctcgatc gcgcccattt gagtcgtggc gatttcgtcg agcacctttt cggcgccacg
     9481 taaatatgaa tagccaagca ctgcctagcc cacctccgga caatccgtcc tgctcgcgaa
     9541 tttgctacag aatcgagaat tctgaactta attcgatttt ccttcatggt atttgtgaca
     9601 aatcgagtct gaaggacaaa gatctgtgcg gcaatgggaa tacttcagaa ggtatatgta
     9661 tatcaatgta gaataaatct acaaatcaga gtggtgtgtc cgtatgtcga actgacggat
     9721 aggtgagtgc ttcagggggc cagcacgtga cagaactgga gcgagggtgg tcgcctgaaa
     9781 ttggcaggaa gcgaaaggga cgtggcatgg ctatatttcg gcgcctatcg ctgtagccat
     9841 aaaggcacct cgtagattct cggatttggt gtacatatcg gcgcattgcg gcgtatctcg
     9901 cgttcgcgtt tatttgaaaa ctctccttca aagggataga atggcatgtg catacattca
     9961 tagtaatgaa ctgtaatctt gacatctgag gatggaggcg tatttgaagg gtgtgatgtt
    10021 atcttcatcg gagtgatgca ggaaaaaaat ccagtgattg gaggggattg atgtgatatc
    10081 tactttggac tttgctaagc ctttagtaaa ccaggcgctt ggatatctac aggcagaccc
    10141 cgtaggcaat ctgccgaagg cgcttgcgat tgcggaaaaa ctggcgtccc aggagaatca
    10201 caagcaacag gtccgagaag tcggtaaggc tattcttgac ccagatagca actggcgtga
    10261 gcttgctatt cgcttattaa cggagactca tcccaatata atgaagggca ttggagttaa
    10321 tttcttcgtc aactctgcgc tcgttggagt gccgacacag tatgatctcg cagaagaact
    10381 gggcgtacag gttccttacg cgatactcat ggaccccacg gaaaagtgta atctcaggtg
    10441 tacagggtgc tgggctggcg actaccagcg cgtccgtgaa ctcccgtttg agaccatgga
    10501 tagggtatgc accgaagctg aggaattagg catttatctt atagtagtat ccggcgggga
    10561 acccatggtt gccaaggata aactgatccg tctggctgaa aagcatccga atcagctgtt
    10621 tcatccgttc accaatggca cgctgttcga cgaggaattc gtctccgaaa tgatcagact
    10681 gggcaacatt gctcccgcta tttccgtgga aggtctcgag gagcagacag acgcaaggcg
    10741 aggaaagggc gtctatcaga agataatgaa caccatggat atgctgcgcg aagccggatg
    10801 cgtattcgga ttctccgcat gctatgcccg caacaacaca gagataatcg ccagcgatga
    10861 gttcatagat accatgatcg ataagggttg ctcgtttgga tggttcttca cgtacgttcc
    10921 tgttggatcc gaccctaacc tcgatctgat ggcaactccc gatcagcgtg ctctgatgta
    10981 tgacacagta cgtcagtggc gtttcacgaa gccgatcttc atggttgact tctggaacga
    11041 cggcgagttc gcgctcggat gcatagcggg tggcaggcgc tatctgcata tcaacgcaaa
    11101 tggcgacgtt gagccgtgtg cattcattca ctatgccgtt gataacataa acgagaagcc
    11161 gcttaaggag tgcctggctt cgccactgat gaaggcttac cagaagcggc agccgttcaa
    11221 cgacaacatg ctgaggccgt gcccgctcat cgacaatcct gagaagcttg tcgagatcgt
    11281 ggaagagtcg ggtgcgcatt ccacacagct tggagagcat cttgtggatc acagagagct
    11341 tgcagagatg atccaggaag gatacaccaa gcattgggct ccgaaggctg atgaaatatg
    11401 gaatgcgaat ccgcatccgt ttttcgagat gcagaataag tactacgagg aagagcggac
    11461 tgaggatagg aagaagcaaa aagaaatcca gagcaaacag ggctaaacag taacccaaca
    11521 gaatagcatt gaaagcccgc gtctgagacg cgggcttctt gccgcgttac gtctgtctaa
    11581 tgaggcctct cgcgaagtcg gcgccctgtt ttctgcatgg ttttccgtag cgttactcct
    11641 ttccgagcag aaccggtttg gtcaacgttt gaagaataca gtagcgcgct ccagcgtcta
    11701 atcccgtctt gacctcgtaa aactcttgta tttgaggaga cttgctgcgg ctctgaataa
    11761 cgacatatgc ttcatgcaca atgagggcta tacggttcgg cagcccgatt ctgcaaggga
    11821 gagtccgagc agtccagcgg gaatgggaca tgccctcaga catcagcttt ctgattgctt
    11881 gaaatgatcc cgccgcccac aagcaaatcc ccgtcataga aaacagcaga ttgtccagga
    11941 gtaattgccc gctgaggctc gttaaactct acgcgcactc tcccattggc tgcaggaaat
    12001 attactgctt tggctgccgg cgatctatat cttatttttg cctgcacgtc catcttgtcg
    12061 cttagtgtgt cgaaggggat gaaattagtg ctctcaacct cgagccagct gctgtagagg
    12121 cgttcgacgg ggccaactat tattgtgttc gagtcgggtc gtatctccaa tacgtaaaga
    12181 ggccgatcgg ctgcgagact cagtcccttc ctttggccaa cggtatacat ggctatgcca
    12241 cggtgctccc ctataacgtg gccctctgtg tctacgatgg gaccgggagt ctggaaggaa
    12301 ggatcatttg atgccaagaa ctcccgatac ttgcccgggg gaagaaagca tatctcctgg
    12361 ctttccggtc ggtctgccgc ttcaaggccg atggacttgg ctattttgcg aacctggacc
    12421 ttttcgagat cgccgagagg aaagactgaa cgcgccagct gttcctgccc gagtcgatag
    12481 agcatgtatg tctggtcttt gcgagaatcg gccgggcgga gaagcaggta tcttccggaa
    12541 accgcatcct gtcgaagcct tgcgtagtgt ccggtagcaa ccatgtctgc gccgagttct
    12601 ctggccttgt gatgcaatag gtcgaacttg atcagctgat tgcatatgac gcacgggttc
    12661 ggggttgatc cgccggcata ggcgcggacg aagtccgcaa caacagtctt cttgaattcc
    12721 tcggacaggt ctacaacgac atgatcgatg cctagcgatc gagcgacccg agctgcggaa
    12781 tctgccgcct cttcgccgaa gctgtccccg cacgcatctc cttcgaaagg gccgggccaa
    12841 tgccaggttg tcatcgtgat tcctatcacc ctgtgtcctt gggctacgag gagcgcggcg
    12901 gcgactgacg agtctacgcc cccgctcatg gcaactgcta ttgtctgatt gttgatattc
    12961 gggcccatgg gcgcccctcc tcttaccgcg tgagtccgct gtgcttcaca attgttctgt
    13021 tgtgcttgcg gttgcgtcaa ccatgattac tatagcccac tttcgggtct gcgtggaaga
    13081 ttcgctgtag ggaaccttgt tcctgatggt catatctgat actattggct agggatatac
    13141 aggcaatgat atcttaatat ggttatacat tatacatagc tattgcataa tccttcggca
    13201 aagcacccca ttattcacga ggccatgaag caggcagagt ccagcatgtt cagaacaaaa
    13261 gcttccggat aggagtgatg cgccggtgta tactgcgagg atttcgattt cgggccatct
    13321 cattgattcg ggtttgctgt cgcggcttct tgatgccgtc tccgatcttg ggggaaagtt
    13381 tgagatcgtc gagactcagt tcgggcggat gaaggaagag gagagctgcg cgactatcga
    13441 ggtgggtgct gacacgtgcg aggagctaga caaaatcctt gacaaggcga aggaactggg
    13501 ggccaccgtc ttcgacggcg aacccgttca gcttgccgtc gtcgagcagg acggtgtttg
    13561 tcctgatgag ttctatacta gcacaaatat tgagacttac gtcaatatcg atgggcactg
    13621 ggttcctgtg gacgatatag agatggactg cgcgattgtt gttgatgctc aaaaaccggc
    13681 agctagatgt tgccctctag ccgcagtttc ccgtggtgag tacgtggtgg ttggaagcag
    13741 cgggataaga gtagcctcaa tggcctcggc caaagagact gaggtcttct ccttcatggg
    13801 cagcgatgta tcgagcgagc ggcctaaggc tctggttgtg gaacagatag cccgggagat
    13861 gaaagaggtg cgggcccgtg gcggaaagat cctagtggtg gcaggtcctg cagtgattca
    13921 cacaggcgcc ggtccacatc ttgccaggct aattgatgcg ggttacgtaa atgtcttcta
    13981 tgccgggaat gcagtggcta cgcacgatgt tgaatgggct ttgctcggta cttcccttgg
    14041 ggtttatctc aaggacggga catccgcccc tatgggccat acgcatcacc taagagcgat
    14101 caacactatt cgaaaggcgg gcgggttgag gcctgcggtg gagaagggga tactgaccaa
    14161 gggggtaatg tactccctag ttactaatga tgttccgtac attttggcag gttccatcag
    14221 agatgacggc ccgttgccgg acgtgataac cgattctgtc aaagctcagg acgccatgag
    14281 gaagaacttg aagggtgtcg aaattgccct gatgctttcg accatgttgc atgcaatcgc
    14341 aacagggaat cttctcccgg cccgtgttaa gacggtatgt gttgacataa atcctgctac
    14401 agtcaccaaa cttgccgacc ggggtacttt ccaggctatt gggatagtca gtgacgtgga
    14461 gtggttcctc aaggaattgg ccgggcatct tgcagcgaat tagcctttag tgcactgctt
    14521 gtgatggtgt aacttggctg cgcatgacag gtccgaaaac ccatatacat gtcatgctgt
    14581 tgcgacagga tatttggctc tgatgtagaa aactgcacaa tgtgggggtg ccttggtatg
    14641 gaggccttat tgcttgtcga tctgcaaaat gatttcttgc cgggcggtgc tctgggggtg
    14701 ctagagggcg atcaggtcat tcctgtcgcc aatagagtac aggaatactt cgacattgtt
    14761 atagcgacag ccgattggca tccgccgggt catgggagtt ttgcctcttc acatccggga
    14821 tgcaaggcag gcgatgttat tgagctttcc ggcatgccac aggttttatg gcctgaccat
    14881 tgtgtgcagc atactgaagg agctgaattc tctcctcatc tgaacactgg caagatcgat
    14941 aaggtgatct ataagggaat ggatccagaa atagacagtt acagcgggtt cttcgataac
    15001 ggaagacaaa aagctactgg tctcgaaacg tatcttcggg atcagaaggt agacacggtg
    15061 tacgttatgg gcttggctac tgactactgt gtcaaataca ctgctcttga cgccgtatcc
    15121 ttgggctaca gaacctggct cattgaggat gggtgcaggg gcgtgggagt tgccgaaggg
    15181 gacgtggagc gcgcgttggc tgagatgaag gcagcaggtg ttcgcgtagt gaaatcggga
    15241 gatctaggtt agccgcggga gtgacctggg gataatgttc tgcaaacgct ggagcgttat
    15301 ctcatgatat accataaatc atggaacagg gagtggctac agatgaaatt ccaaggtttg
    15361 gttatcggag tccccaccga gatcatgaag ggggagcggc gagtagccgc gatgcccgac
    15421 actgtcaaga agatgacagc tgaaggcgca aaggtactgg ttcagaaggg cgctggagaa
    15481 ggttcctatt tccacgatga agagtacagt gcagccgggg ctgagatcgt cgacaatgta
    15541 gaagacctct ttgctagggc cgacatcatt ctcaaggtga aagagccgat tttcaacgat
    15601 gccgtaggca agcatgaagt ggatatgatg cacgaaggcc aatgcatcat caccttcctc
    15661 catcctgcag ctccagccaa tcaccaaatg gttcaggaca tggccgcaaa aggcgttata
    15721 tccctcaccc ttgacggaat acctaggatc tcaagggctc agaccatgga tgccctcacc
    15781 tcaatgagca cggttgccgg ttacaaggct tgcctgatgg cagcaaacag gcttcccaag
    15841 ttcatgccca tggtgggaac ggcagttggc atgatcaagc cgtctacggt taccgttatc
    15901 ggaacgggca tcgcgggtct gcaagcggtt gccacggcta agaggttggg tgctgtggtc
    15961 aactctatcg atatcaggcc cgacgccaat gaacaggcaa agagccttgg agcgaaggtt
    16021 gtcgatgtgg gcgttccatc tgagatcgcc attgcgcctg gcggatatgc ggccaagctg
    16081 cccgacgagt ggctcgaaaa ggagcgggag acagtcaagc ccgtagtaat tgattccgac
    16141 atagtcatca ctgcagccct gatcccgggc aagctcgcac ccgtgatcgt aactgaagaa
    16201 atggtcaagg ctatgaggcc tggctcggcg attgttgaca ttgcgatcga ccagggcgga
    16261 aacgtggaga tcacccagcc tggcgaggtc atagagaaat acggcgtcag tatcgatgga
    16321 acaaagaata ttcctggaat gatgcccacg gcctccacat ggatgttcgc aaacaatatc
    16381 tacaactaca ccgcaaacct cgtcaaagac ggcaagattg acctgaatct tgacgatgag
    16441 atcatcaagt cttccttggt cacccgtgac ggccagatcg tccatgaagg cgctcgtgaa
    16501 gcaatgaaca tgaaataggg agttgtgacg tccgtgtctg aactgtcact tataatcctc
    16561 tttgccatcg ctacatttgt tggctacaag gtgataagcc aagttccaag cctgcttcac
    16621 acgcccctca tgtcgggcac aaatgctctt tccggggtga ctgtgttagg cgcactcagc
    16681 gcaacagcgg cagccaaagc ccagggagct tcggctgtag gtgcagtgct cggatgcgta
    16741 gcgataatta tggcgatgat taacgtggtc gggggcttct tggtcaccga caggatgcta
    16801 aggatgttca aaaagtaggg agggttcagc ggcactgacc gctgtatgat ctgatggatt
    16861 ggaacgaagc aatctttctc ggtcttagcc tggcagactt gctagggctg gtcttcgtgg
    16921 cagggactgt ctggggcatc aggctgatga gctcccccgc taccgccgtt agaggcaacc
    16981 tcataggcgc ggtgagcatg acaggtgcaa tcatagtcac gttggttgca gaggatattg
    17041 tcagcgtaca gattctctgg ttagggatgg cagttggcgg tgcgctaggc tatatccttg
    17101 cagcaagggt ggccatgatc gaaatgcctc agatggtcgc cctgctaaat gggtttggcg
    17161 gaggcagctc tgccattgtt tcactcctta cgttgctggg cccgggggca attgggccat
    17221 tcgaaaaagt aaccgctatc ctcgggcttc ttgtcggcgc gataacgctt tccggaagcc
    17281 ttatagccgc ggggaaactt gcaggcaaga taaatcaaag gccagtcatc ttcgaaaatc
    17341 agacatttgt aaatgctgtc gccatcatca tttcggtggc ggcgggagtt cgggctgttc
    17401 tggctccagc agatgcggtc ctgtggccga gcgtgatcgc acttgtcgcg tcgcttgcgt
    17461 acggggtgct ctttacgata cgcgttggcg gggccgatat gccgattact gtgtcgctgc
    17521 ttaactcgct ttcaggtgtg gcggcatcag tttcggggct tgctattcga aatccccttc
    17581 ttgtggcaat aggagcagta gtaggcgctt caggcctcat ccttacacag ataatgtgca
    17641 gagcgatgaa caggagcctt ggccaggtgc ttacgggcaa gaccactgtg gcgcatatca
    17701 aggggagtga ggcaaaaccg gtttcagccc ctacaaaggc tgcagctggg gagacctctg
    17761 ctcaagcaag actggcgtcg aaggcagaaa agcccgaaaa ggctgactac atctctattg
    17821 caaaggcggc taagaaggta attgtggttc ccgggtatgg catggcgcta gctcaagcgc
    17881 agggcgacgt gaaggccctc atggataaac tggaggccgg cggggcagac gtcaaagtgg
    17941 caatccaccc agtggcgggc cggatgccgg ggcacatgaa tgtgcttttg gcagaggtcg
    18001 atgttccata cgacaagcta tacgagatgg accagatcaa cgacgagttt gccgagacgg
    18061 atctggtgat cgtggtcgga gcgaacgacg ttatcaaccc ggcggcaaat accgcggaag
    18121 gaacgcccat ctacgggatg cccattctca acgtatacca ggcaaagcac actgtaatat
    18181 tcaactacga caccaagccc ggatacgcag gggtcgataa cccgctgtat gaggcggaca
    18241 atgtgacgct ggtccttggg gatgcggcga gcacggtaaa ggagttcatg gcggagctcg
    18301 atgcaagcgg ggctgctgct gagtcgcaac tgtcgccggg caaagttgat tgtcttgaca
    18361 tagccagggc cgcaaagaag gtcatcgtag tgcccgggta tggcatggcg atagctcagg
    18421 cgcagcgcga cgtgaaggcc ctcatggata aattggaggc cgacggcgca gatgtcaagg
    18481 tggcgatcca cccagtggcg ggccggatgc cggggcacat gaatgtgctt ttagcggaag
    18541 ctgacgtctc atacgacaag ctatacgaga tggaccagat caacgacgag tttgccgaga
    18601 cggatctggt gatcgtggtc ggagcgaacg acgttatcaa cccggcggca aataccgcgg
    18661 aaggaacgcc catctacggg atgcccattc tcaacgtata ccaggcaaag cacactgtaa
    18721 tattcaacta cgacaccaag cccggatacg caggggtcga taacccgctg tatgaggcgg
    18781 acaatgtgac gctggtcctt ggggatgcgg cgagcacagt aaaggagttc atggcgaagc
    18841 tcggacttta gccgaactcc atggcgggat agtgtttcca cattagatag ctgcaaaccc
    18901 ttcgcccggc ggaagccgga gcggagggtt tcatttctcg ctgctatgac attattgttc
    18961 ccgcagggga accgatcggc ttaggtaaag gaatgtgtta ggatcccaac ccccgcctta
    19021 tgaagcaggc agtcaagccc taatgctagt ttcgtcagtg gcgccgaaga cctagggttg
    19081 gcagactcat gtccgttttc cctacgccct catctaggaa attgaccagc atgattccga
    19141 gccgtatttt cggacgcttg gcgggccagg a
//
LOCUS       DAOSYL010000046        18693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2457_length_18693_cov_8.062721, whole genome shotgun sequence.
ACCESSION   DAOSYL010000046 DAOSYL010000000
VERSION     DAOSYL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18693
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_18693_cov_8.062721"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1030
                     /locus_tag="PLU15_07915"
     CDS             329..1030
                     /locus_tag="PLU15_07915"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOO30887.1"
                     /translation="MEFLANQGKRLSVEIDGRTYMRIPIKTHVVVADDDIVDVVGRYA
                     EPLLCTGDMLVVSEKIVAITQHRAYPVDQIEPSRLARFLSRFVYKSPYGIGLASPCTM
                     ELAIREAGAPRIVAAACVAGVGKLFGAKGVFYRICGANVAAIDGPCDYTIPPYDRYAI
                     LAPSKPKDVAQSLSDATGMPVAIVDANDLGVSVLGLSAGCPEPELIKKILADNPLGQS
                     CEQTPIGIVRQAKDI"
     gene            1502..1888
                     /locus_tag="PLU15_07920"
     CDS             1502..1888
                     /locus_tag="PLU15_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOO30888.1"
                     /translation="MGMPPLGDQELELLRFITEHSPITVREAYENYGKDRGLARTTIL
                     TMMERLRNKGYLTRSEGKGANEYSACLTRSALMRGVVRGFVEETLGGTLSPFVAYIAE
                     EASVSPQELEELKRLVEELESREEEN"
     gene            2062..3024
                     /locus_tag="PLU15_07925"
     CDS             2062..3024
                     /locus_tag="PLU15_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOO30889.1"
                     /translation="MFLQLFAAPQIDLAVLPRTRTVIAHVVSYRFAEQIPTRGDLARG
                     VGGPLMALWIIGVTIGVAGVAIAYAKARSMALRSVSVRREELDAMVARICRNMKISCL
                     PELVVAGWASVPMTLRRRSRHVVVIPLKFLAPERQSDLELALAHELAHIKRHDLNWNW
                     LPTAAKILFFFNPLIWVVDRELGIAQEMACDEMAVRATRSKAARYGRVLLTMVRPDRE
                     AGSTWRVATASAGASAEVIRRRLVELGHMQDPPARRVMASIVTLLFMCLLVMPFQIVE
                     APRDGLPRPGVSRIRDKIYISEPWGEKASLDGVTIGIDFEPSGK"
     gene            complement(3539..4663)
                     /locus_tag="PLU15_07930"
     CDS             complement(3539..4663)
                     /locus_tag="PLU15_07930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOO30890.1"
                     /translation="MIRRKTFPLVLISLSLILVLWAQPGAPGMSSRFSFAAVGDALIH
                     QGVYNAARVEDDAFDFRPMLRLIADRIARHDLAFYNQETILGGVELGLSTYPRFNSPQ
                     EVGDAFIDTGFNLVSLANNHTLDRGENAVLRSLEYWSGKESVLTAGSYFSQTDRDEIQ
                     TVEVNGITCAFLAYTTLTNGLRPPNGKAYYVNVYDPETARADVEKARANADVVIVSMH
                     WGDEYVFAPTKEQTEIAEHLAELGVHIIVGHHPHVVQPVTMVGDTLVIYSLGNFLSAQ
                     KELFKLVGLLVSLDVVKTETPEGVKISLENVTGTLLYNPRHSPLGRYVVVPFDMMTEA
                     ILKDFDKVHSRFSAYVKKDDTIAIEPPADAHEQECVDCGS"
     assembly_gap    4903..5002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5304..5810
                     /locus_tag="PLU15_07935"
     CDS             5304..5810
                     /locus_tag="PLU15_07935"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOO30891.1"
                     /translation="MDAEYFVDLALEWGADHAVPFEISDIVFDPRTILKCAFGCGDWG
                     YGHTCPSRPLSLRPWEYEQVLRRYSWGIIIHSTDKRISQDVSFGIEREAFLRGYYLAF
                     SLSDCAICGQCAGFVGKPCANPQKARPAFHSVGIDVFKTVHRFGLPLDTLKSEDETPN
                     WYSAVFIE"
     gene            7000..8196
                     /locus_tag="PLU15_07940"
     CDS             7000..8196
                     /locus_tag="PLU15_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO30892.1"
                     /translation="MKRSKTAVIIVLLIAVAGGAGFMWWNRSRSKPGAASNPYIPIEV
                     RRGELSTDIYATGNVQTIRTVDVYPTKAGIVAQVDVDEGTEVRAGDVLIRLERDELEV
                     QQTEAEVRQKRENLASSEVALEKIRSLHAKGAATEKELRSAESDVLQAKESYNAAALK
                     LDRLVTKASDSVIRAPISGVVSSVKATVGAGVSTSNPVAVITDLSDVVVRVTVDEYDI
                     GSVEVGQGAEVVMDALDDRKYAGNVCFVGKIGQNQSGVVVFNVDIRLDNPTQDVRPGM
                     SAEASIMVDRVENALIVPNSVLETRRGETVARIYNADGSVQLRPVIVGLATDGGTEIV
                     DGLNLGDMVAVPNPRAVSSNEDQGSAGREPEQGMPGMMPGMPGEAPTGVPGMGGPSGG
                     FAPPQR"
     gene            8208..8906
                     /locus_tag="PLU15_07945"
     CDS             8208..8906
                     /locus_tag="PLU15_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30893.1"
                     /translation="MSLIELKEVSKIYSMGKGSIEVRALKGVDLAVDEGEFVSIMGQS
                     GSGKSTLLNIVGCLDKPTSGEYWLDGCLVGSLGSDELAEVRNKIMGFVFQSFNLLPKL
                     TAVGNVELPLVYAGVHPRIRREEAIRALDMVGLADRASHRPNELSGGQQQRVAIARAL
                     VSNPRVVLADEPTGNLDTKSSYEIMDILADMNKRGITVVIVTHDPDIAAYTPRVLTFR
                     DGECIEDVRALEGV"
     gene            8911..10083
                     /locus_tag="PLU15_07950"
     CDS             8911..10083
                     /locus_tag="PLU15_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30894.1"
                     /translation="MRPADYLRLALSGVTSNPFRSILTALGVIIGVSAVIITVSIGTG
                     AKVQTQSQIQRLGSNLITISSGYRSLRSVITSDILPIIESINTVGEVVPQVQRQSTVV
                     HGAEGLSTTVTGTSANYPSVRNLKLAQGSFLTDADIDKGSWNCVLGADLAEELFGDDN
                     PLGQKVRLDRVRFTVIGVAQRQGDSGFASSDNAMYVPYTTMQRRITGSKNINTIIAQA
                     VDASSMDLAYSQIYAMLMMATGDEDSFRISNQADMLEVASDVTDTMTLLLSGIAAVSL
                     VVGGIGIMNIMLVSVTERTREIGIRKAIGAKEHEILSQFMLEAAALSLAGGLLGIVVG
                     LVGSRIVSQVLSWPTAVDMKSVALGFTLSLIVGVFFGAYPAYKGAKLDPIVGLRYE"
     gene            10106..11485
                     /locus_tag="PLU15_07955"
     CDS             10106..11485
                     /locus_tag="PLU15_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO30895.1"
                     /translation="MRASVGRRMPIFIIMLILAASSGATADGTFGARWSIDTFADAAV
                     NVWKNSPQARQPLELALIGEGLSVPVRSASITISSGMSSASQLTESTGDRGDAEKVSA
                     KLPITDRISLNAAYDLNKSSGSLKLSYTLPHTWLAGSDEGIAQRLFGHSGASEDAKVS
                     TDMARMKLDASESSIKLKAQKAYIGALKSIKNRETAEEEWRLAQTALEIATKRCQVGL
                     STERDVEQAELSVLDAEIALVKAENEERWARRELSKLAGVDMLEAELEDLPQFSLDMP
                     SLDVMIEAAFESDVELAQAELGLDSSIRELEAIKSLLPTVSAEVQFPSGSSVPKFVAS
                     ATWSISLSRSLEAQKAEINVDQKRQSVTERREALAENVEKSLEQLHISVWSAAKWVKQ
                     LEQSQESYDTALKSYKDGELLLVDLERAQLNLQKARNSYIANWYGVWELWYTLANTCR
                     VEDLGMGNR"
     gene            11515..12606
                     /locus_tag="PLU15_07960"
     CDS             11515..12606
                     /locus_tag="PLU15_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO30896.1"
                     /translation="MKRNCMLKMVPVLLAVTLMLSGIALAGEYVDETLEVTLAQYVEA
                     YVGNSDAVKAAREDAENAQTQWQRAVDEKKAALTVKELENAAETKRLALDEAVNNAAL
                     QAVQAYVGLGQALRELEAKRVSLAVSQERLRVTNLRYEAGLTTKDAVLEQENSYLSAS
                     DSLLKAEQSLRQTKRSFCQNIGAELCDDIVLATDVSTLYGGVPEYGLNDSIEAAQGVG
                     SSYFSAAAGKELARRKCEALQDPLIATPTERETAEKSWKDAETRFASAEQSLIDSIEN
                     ALVELESLVRNLNMQTISAQRAQSNLEVARIKFQHGEMLQYELDNETSSANQALYRMA
                     KAKEDLFVHMLKLESLIGGSVLDIVMSKK"
     gene            12617..13291
                     /locus_tag="PLU15_07965"
     CDS             12617..13291
                     /locus_tag="PLU15_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO30897.1"
                     /translation="MTKVLLADDEARMRRLVSDFLKREGFVILEAENGREALDLFLSD
                     QDIELVILDVMMPGMDGWVVCREIRRRSQVPIIMLTARAEESDQLFGFDIGADEYITK
                     PFSPMILVARAKALLRRTQAGDGGLRHLGLLEIDEGRHVVSVDGERLELSPKEFELLV
                     YFADNANLALSRDQILSSVWDYDYFGDARTVDTHVKRLRAKLGPAAGYIETVRGIGYR
                     FEATEE"
     gene            13288..14838
                     /locus_tag="PLU15_07970"
     CDS             13288..14838
                     /locus_tag="PLU15_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO30898.1"
                     /translation="MKSIRARLFLGIVGLVVGYVLLSWLLNSQLLDVYYHRIKTNSLV
                     SGYKHVKQAYDSGDLAQLLLDLETMERKEGLMVVIVDSSYTLQYASRPVQSARHGSAG
                     PTGPMDSALLDSLDQQRMPPPGPGPNPAPNPDPFPVNTIERAIADLEGEYEVLSFTRD
                     DRLKLDFISLIGRLPDGGYVFLSAPISSIEESASAANRFFLVSGVLMIAVGSIAAYWY
                     AGRFTQPIHDMKVIAERVAKLDFSMEYVGSTDDEIGALGESINIMSRQLEYSINELVR
                     ANERLQQDIERERQIDEMRKEFIANVSHELKTPIALIQGYSEGLKLNVNDDEENRDFY
                     CDVITDEAARMNRLVGQLLKLAQIESGNIAPRKICFDIVEFINHIVRKNGLALQERGV
                     DLGIEGESCVEVVADIDMMEQVLVNYITNGIDHCDGERKMTITIEEVDEKARISVFNT
                     GQHIPEDCMDSIWTSFYKTDKARTRDHGGTGLGLSIVKAVQKAHGNDCGCKNVAGGVV
                     FWFDADLC"
     gene            complement(15360..16202)
                     /locus_tag="PLU15_07975"
     CDS             complement(15360..16202)
                     /locus_tag="PLU15_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO30899.1"
                     /translation="MNRRAVRAIARKDIMSITSNIQVWLPMLIVPLVFAVLFPIGASF
                     AIRATDTSTSSDFQELLDVLEKLPQSSLDSLNGIFDSLITDQQRIAYMMFNYFMAPFF
                     LMIPLMAASTISVDSFVGEKERGTLEGLLLSPVDMRSIFAAKALASLIPAIAISLTSF
                     VLFAVCANVFAWPIFGRLFFPSIAWAPLILLVMPAIGLLAVLINVFISARVSTFQAGY
                     QMSGFLVLPVIALMIGQFTGIMLLDAKVLLAIGAALIIVDYALLMAITKRLDKAQIFE
                     SQVR"
     gene            complement(16195..17097)
                     /gene="ccmA"
                     /locus_tag="PLU15_07980"
     CDS             complement(16195..17097)
                     /gene="ccmA"
                     /locus_tag="PLU15_07980"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOO30900.1"
                     /translation="MIQIKDVTKRYGEQRAVDALSFNVARGEILGLIGPNGAGKTTTI
                     RMLCGVVKPDAGQVRVAGLDPQSQGDEVRSQIGVLTETAELYSNLSGIENLRFFAELY
                     GIDDPRRPHELMEEFGLADHKEKKAGAYSTGMKKRLGLAKALLANPPILLLDEPTSGL
                     DPAGIAMVLDEIERLNSQKGVTVVLCSHVLHQLETVCQRYVIIDRGRTVAQGTLPELA
                     EKCPGPTIVEVDADYILDGEAYRGIPARPLPVGDIQFTVSSRNEVPKLIRQLSQDADV
                     YSVKLKGHDLESVYFRLLEGEKDE"
     gene            complement(17192..17458)
                     /locus_tag="PLU15_07985"
     CDS             complement(17192..17458)
                     /locus_tag="PLU15_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30901.1"
                     /translation="MNELDPILWIFLGALLIAQATWIFRDARLRREDYAWLWGLFGLI
                     NIPSSIIVYILVTRRNNVRCAGCSKPIPRELEICPYCGSIKAEK"
     gene            complement(17455..17961)
                     /locus_tag="PLU15_07990"
     CDS             complement(17455..17961)
                     /locus_tag="PLU15_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30902.1"
                     /translation="MNIDCRNFQDMLLDMAYPESGEADDQGRLDKDISTTDVARHLKN
                     CPECRDYADALVDIASLLPSSHVNVPSGSAERPATTDLGIEGIAPAWPRLDATIERGI
                     KMRIARERRNMALFALTATGILTSVWVPLLASKPGAFLGLQAIGFFGIALFYLPLHVL
                     REGRGDVA"
     gene            complement(17958..18497)
                     /locus_tag="PLU15_07995"
     CDS             complement(17958..18497)
                     /locus_tag="PLU15_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO30903.1"
                     /translation="MPDETSLVRRAQQNDRQALEELFWINYPILLGALIRITGDPRSA
                     EDIAQETVLRAIVNLERYEPRAKFSTWLITIALNTHKNKLRTARREILTEDVFETGSE
                     EESAEEKALRNMAVDEAMSLLDALGPEKRQVFVLRHYYGFKYEEIADAVGCPIGTVRS
                     RLHDSVEILRREISRRDMK"
BASE COUNT         4392 a         4460 c         5439 g         4302 t
ORIGIN      
        1 gccaccggct acgatttgcg gcaggtttgc atcgctccat gaattatcgc cactcaggct
       61 ttgactgcaa gcgtgatgag catgttcaaa tcctcgttga gacatgatgc agacttgtag
      121 aaaggcttct caaggcttgc gttattctga tcggccgcaa agccagattc gacagccaag
      181 acagttgact acaacactag tctgtgctaa tgttgactac agatgtaggc acactggtga
      241 ctgatgcggg gggccagcgg agttgcagct agaaagctgg attcagaagt cctatttgca
      301 tggagcagca aaggggatgt tgaaagtgat ggagtttttg gcgaatcagg gtaagagact
      361 atcggtggag attgacgggc gcacctatat gcgtattcca atcaagactc atgttgtcgt
      421 cgcagacgac gatattgtgg atgtggttgg gaggtacgct gaacctctgt tgtgtacagg
      481 cgacatgttg gtagtgagcg agaaaatcgt agctattact cagcacaggg cgtatccagt
      541 tgaccaaatc gaaccttcgc ggcttgctcg ttttctctct cggttcgtgt ataaatcgcc
      601 ctatggaatc gggctggcga gcccgtgcac gatggagctg gctataaggg aagctggcgc
      661 gccgaggata gtggcggcgg cgtgtgtggc aggggttggc aagcttttcg gagctaaagg
      721 agtcttttac cggatatgcg gcgcaaacgt ggccgcaatc gacggcccat gcgactatac
      781 tatcccgccc tacgacaggt atgcgatttt ggccccgtcg aagcccaaag atgttgctca
      841 gtcgttaagc gatgctactg gtatgccggt ggcgattgtg gatgcgaatg acttgggcgt
      901 ttccgtactt ggattatcgg cgggatgccc cgagccggag cttataaaga agatccttgc
      961 cgacaatccc cttgggcagt catgcgagca gactcccatt ggaatagtgc gccaggctaa
     1021 ggatatatag gttatagcgg aactttcaag attcgacgtt ttctcggcgt tcaaagcggc
     1081 ctgaagaaac agtgatagcc gtggttggat gtgtgtttct tgtaggtgac gacgatcggc
     1141 aataaaggga gaacaacgtc aggcgcccac ctcgtggcca agacagaatg tggcaagccg
     1201 agttgactgc ttagcgatta cacagagaca gacagcgggc aatgcacatc gattagcacc
     1261 tatctcaatt taatgtgggc acgtgtgcca tggtgctctt ttggctgctt tggggaaaac
     1321 acagggtttg cgttaacccc caaactaggc ccgcgtgacc ttagaagagg tgacggcagt
     1381 tacggttgct acaatgccca cgtgcactcg gttgcagttg acgccctctg attccatgcg
     1441 tatactgact acagatatag ttaggagtgc tttaagacga cgagatgggg ggtatgggca
     1501 tatgggaatg cctcctctgg gcgatcaaga gttggaactg ctacgattta tcaccgaaca
     1561 ttcgccgatc acggtgcgtg aggcttatga gaactatgga aaggaccggg gccttgcgcg
     1621 aaccacaatc ctcacaatga tggaaagact cagaaacaag ggttatctga ctaggtcgga
     1681 ggggaaaggg gcaaatgaat attctgcctg cttaacccgt tcggcgctca tgcgcggagt
     1741 ggtgcgtgga ttcgtggaag agacgcttgg aggcactttg tcgccttttg tagcatatat
     1801 cgccgaagag gccagtgtaa gtccgcagga acttgaagaa ctaaagcgac ttgttgagga
     1861 actcgaatcg cgggaggaag agaattgagg ggctgtctgc agtatctgtt ccaaatctca
     1921 tcccaatact gggcaagttc catgtggagg gctttctatc aggggagcct ggcgctcgcg
     1981 tttgtatggg ctgtatgcaa agccttcccg cacatccccg cccgtgtcag atgctggcta
     2041 taccgtcttg gattggccaa attgtttctt caattgttcg cagcgcctca gatcgatctc
     2101 gctgttttgc ctcgaactcg aaccgtaatt gcacatgtcg tgtcatatcg ttttgctgag
     2161 caaataccca cccgcggaga tctggcccgg ggggttggcg ggcctttgat ggctctttgg
     2221 attattgggg tcaccatagg cgtggcaggg gtcgcgatag cttatgcaaa ggcgcgctcc
     2281 atggcgttgc gcagcgtgtc agtacgtcgg gaagagctcg atgccatggt ggctcggata
     2341 tgccgaaaca tgaaaatcag ctgcctgccc gagcttgtag tcgcaggctg ggcatctgtc
     2401 ccgatgacat tgcgaaggag gagcaggcat gttgtggtta tccctctaaa gttcttggct
     2461 cccgaacgtc agagcgacct ggagttggcg ttggcccatg agttggccca cataaagagg
     2521 cacgacttga actggaactg gcttcctacc gcggccaaga ttctattctt ctttaaccct
     2581 ctgatctggg tggtcgatcg tgagctcggt attgctcagg aaatggcttg cgacgagatg
     2641 gctgtgcgcg ctactcggtc taaagctgcg aggtacggca gggttctcct cacgatggtc
     2701 agaccggatc gggaggcagg ctccacatgg cgagtagcga cggcgtctgc aggggcgtct
     2761 gccgaagtaa tacggagacg cctggtcgag ctaggtcaca tgcaggaccc gcctgcaaga
     2821 agagtgatgg catctatagt gacgttgctc ttcatgtgtc tgctggtgat gccatttcag
     2881 attgtagagg ctccacgcga tggcctgcct cgtcctggag tgtcgaggat cagagacaag
     2941 atatacatat ccgagccttg gggcgaaaaa gcatccctcg atggagtgac tataggcatc
     3001 gactttgaac cttcaggcaa atgacggttc aaagaagaca ttgccatggc catcaagtcg
     3061 cagccgactg acgctaccgg ttgccctcaa cacttccata agagatccac tggccaactt
     3121 cgggcatttt tgcggacgat tgggttttga accgttcgcc gacagatgct tcagactgtg
     3181 tcgataaaat cgcgctgagc atgtagtttt ggtagtatgc agggtagcga gcaacttgta
     3241 tcttcgcagc ctagtcgggc ctcttgctca tctgcattcc aatatttggt tactcataac
     3301 catcgccacg gcttctgttg cgaggacacg cgtgttcgca gagccgcatg gtgcaagagg
     3361 cacggtcttt aggaagaatc ggtattccct aaagaatcag gaagtgaagg tagggaggcc
     3421 tgcagggcgc tatagactgc caaacggacg gccttgacgt tgtggtccag ggtcttggcg
     3481 tgcgagttga agctcgtatg tgagcccgca ccaaggtatt gaacgtccag aggctgtctc
     3541 atgaaccgca gtctacacac tcctgttcat gcgcgtcggc agggggctcg atggcgatgg
     3601 tgtcgtcctt tttgacgtat gctgagaatc tgctgtggac tttatcgaaa tccttgagga
     3661 tcgcttcagt catcatgtcg aaaggaacta ccacgtagcg ccctagaggg gagtgacggg
     3721 gattgtatag gagggtcccg gtgacgtttt ccagactgat cttcacgccc tcgggcgtct
     3781 ccgtcttgac tacgtcgagt gagacaagaa ggccgaccag tttaaacagc tccttttggg
     3841 cggagagaaa gttgcccagg gagtagatga ccagagtatc gcctaccatg gttaccggct
     3901 gcactacgtg agggtggtgt cctactatta tgtggacgcc gagctccgcg aggtgctccg
     3961 ctatctccgt ctgctcctta gtaggtgcga aaacgtattc atcaccccag tgcatggaga
     4021 cgatgaccac atcagcatta gccctggctt tctccacatc ggccctggct gtctcggggt
     4081 cgtagacgtt cacgtagtat gccttgccat tggggggtct caggccgttg gtgagggtgg
     4141 tataggcaag gaacgcacac gtgatgccat ttacctccac cgtctgtatt tcgtcccgat
     4201 ccgtctggct aaagtagctt ccggctgtga ggacgctttc tttaccgctc caatactcca
     4261 gagagcgcag aacagcattt tcccctcggt ccagggtgtg gttgttcgct aacgatacga
     4321 ggttgaaccc ggtgtcgata aaggcgtccc ctacctcctg aggggagttg aagcgtgggt
     4381 acgtcgacag accgagctcg acgccgccta ggattgtctc ctggttgtag aaggcgaggt
     4441 cgtggcgggc aatgcggtcg gcgatgaggc ggagcatagg gcgaaaatcg aaggcgtcgt
     4501 cctctactct ggcggcgttg tacacacctt ggtggataag ggcgtctcca acagcagcaa
     4561 aggagaaacg ggaagacatt cccggggccc cgggctgtgc ccagagaacg agaatgaggg
     4621 aaaggctgat aaggacgagc ggaaaggtct ttcttcggat catgatctcc tccatgagca
     4681 tgcgtttcaa tcaatagctc aattcgcccc gctcaggaca tactcctctt cgcgttagcc
     4741 gggtgttgtc atgatggggg atcctgacac agctttctcc tctaacctcc ttgagtttgg
     4801 tataatcata acatcagcag ggcttcatgc ccgaggcact ggggtattct gagcattcct
     4861 aagcggccaa ggtctgaatt tcagacggag tcaggttgtc gannnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nngcgctcat caactcgaag cgcgtgagcg ttggcgatgg
     5041 gaattcggga tctcctaggt ggcaagccat ccgtacggga agtcgctaga tgagaatgaa
     5101 gcggagaaaa tgtcgagctg gaagaggctt tacggatact gtggcaatag gatgagtgcc
     5161 ccggcttgtc tcaaacgagc ccgacggtta gcgtcggatt tgtttttgcc tgaggaggcc
     5221 gcggcattgg ttacgggatt aagttgagga atcctatctt ggagacggac caagttgaga
     5281 cttccaaaga ggtgtagtgg cttatggatg ccgagtattt cgtggatctg gccttggagt
     5341 ggggagctga tcatgccgtc ccctttgaaa tcagtgatat cgtattcgat ccgcgcacta
     5401 tcttaaagtg cgcttttggt tgcggtgact ggggctatgg gcacacttgc ccctctcggc
     5461 ctctttctct taggccttgg gagtatgaac aggtgttgcg ccgttattct tgggggatta
     5521 tcattcattc tacggataaa cgtatttcgc aggatgtctc cttcggcatt gaacgcgaag
     5581 cctttttgcg cggatattat ctggcctttt cacttagcga ttgcgccata tgcgggcaat
     5641 gcgccggatt tgtcggtaag ccgtgcgcca atccccaaaa ggcgcgtccg gcctttcaca
     5701 gtgtgggcat tgacgttttc aagaccgtgc accgcttcgg cctgccgttg gatactctca
     5761 agagcgagga tgaaacgccc aactggtatt cagcggtatt catcgagtga taactggatt
     5821 gtgccgggag agttgtctat cacggcaatc aggcatgctt ggcttcccac ggggatgtta
     5881 tagctgagca acccgacagt gaacagtttt cagcttccat agcccaagtt agctaatatg
     5941 atggctgaca gcttaaggtt acgaaagcct agatgcttgg atgttccagt atctttgtct
     6001 gaagatacgt agattggcgg aacaagcaag tcatggaggc tgctacggat cacagaagcg
     6061 gcagcagaag tccgttcacc gaaggatata gacgatgcgg ttacaaaccg tagttcaaag
     6121 ggtcatgaat acagcaaaaa ccgttgggac attcgtattc tccagagctg aacccgaaaa
     6181 agaacatgtg ggccgggccc cgagaatact ctgcccgaaa cagtgcgtat gcagttaaga
     6241 gggagccttc gtatacgcgt acaacacacc aagcaaaatg agaaggtgtg tggacgttag
     6301 atcgtaattt ggggcatcat ggattcactt tgctgattga ggattatcgt ccgtccaacg
     6361 gaaactcccg cttcacgttc taggcgatag atgactcagt acgggccatt tggtcagggc
     6421 ttagacactt atcgacaatc cccgcttcag gctcgcacat cacatgattg agattgcagc
     6481 taagaacccc tccatcacgc tctttgactg gccatacagc aatatggtaa tcactggcgc
     6541 tatgacaaaa ccgtgtcggg aaattcacag gtcacaggta tgcacccatg aaacggggtc
     6601 tagtgctact cttagtttga atccgatcta gcccaatcgc ctcctttccg cacactgtaa
     6661 ttggttgttc tacaaagctg gtgcgcaggc ttcctaaacc cgcctaaggg ccacaatcct
     6721 gacccaccac agatcaagat tacatgaaga gcgctgatgc gttgcactat ccagtcttcc
     6781 ctgaggcacg tttttactta tccctccaat atcttcacat tcacatcgcc cattttctgc
     6841 tttcggacat ttggcactca gaagtgttca gtgtctcttc gaagccgtcc gggaacccta
     6901 gaggctggaa gagaaggcaa aagttcatca tgtgttcaca ttcccgacac atgacgctgg
     6961 taatctgtgc tcaggcggtg agtgatggag gtcgaaggca tgaaacgaag caaaactgct
     7021 gtgatcattg tgttgctgat tgccgtggcg ggcggtgccg gattcatgtg gtggaatcgc
     7081 tccaggtcga agccaggagc cgcgagcaac ccatacatac ctatcgaggt cagacgggga
     7141 gagctctcaa ctgacatcta tgccacaggc aatgtgcaga ccattcggac tgtcgacgta
     7201 tatccaacca aggcgggcat cgtcgcccag gtggatgtgg acgagggaac cgaggttaga
     7261 gcgggggacg tgttgatcag gttggaacgc gatgaactgg aggtgcagca gactgaggct
     7321 gaagtgaggc agaagcggga gaacttggcg tcttcggaag ttgccctcga gaaaatccgg
     7381 agtctccatg caaagggggc ggcgactgag aaggaactca ggtcagccga gtcagatgtg
     7441 ctgcaggcca aagagtccta taatgcggcg gcgctaaagc tggatcgcct tgttaccaaa
     7501 gccagcgaca gtgtgatccg ggccccgatc agtggggtgg taagcagcgt gaaggcgaca
     7561 gtcggcgcgg gcgtgtctac atccaatcct gtcgccgtaa ttacagacct cagcgatgta
     7621 gtggtcagag tgaccgttga tgagtacgac ataggctctg tcgaggtagg gcaaggcgcc
     7681 gaggttgtca tggacgcatt ggacgatcgg aaatatgctg gcaacgtgtg ttttgtggga
     7741 aaaatcggtc aaaaccagtc cggcgtggtt gtattcaatg tagacatcag actggacaac
     7801 cccactcaag atgtacgccc ggggatgagc gctgaggcca gcatcatggt ggatcgagtc
     7861 gagaacgcgc tcatcgtccc caacagtgtt ttggaaacgc gcagaggcga gactgtggcc
     7921 cgcatctaca atgctgatgg cagtgtacag ttgcgccctg ttatcgtcgg tctcgcaact
     7981 gatggcggaa ctgagattgt ggacggtctg aatctcggag acatggtggc agttccaaat
     8041 cctagggctg tatcatcaaa tgaggatcag ggctctgcag gacgtgaacc agagcaaggc
     8101 atgccgggga tgatgcctgg gatgcccggc gaggcgccga ccggagtccc tggcatgggc
     8161 ggtccgagcg gtggattcgc tccacctcag agataggggc gatggcaatg agtctcatcg
     8221 aattgaaaga agtcagcaag atctactcca tgggaaaggg atctatagag gttcgtgcat
     8281 tgaaaggagt cgacttggca gtggacgaag gcgagttcgt gtcgatcatg ggccagtcgg
     8341 gatcgggcaa gtctaccctg ctgaacatag ttggatgcct tgacaagccc acctctggcg
     8401 aatactggct ggatggctgc ttagttggga gcctcggctc agacgagctg gcagaagtta
     8461 gaaacaagat catgggcttt gtctttcagt cctttaactt gctgcccaaa ctgactgccg
     8521 taggcaatgt ggagctgccg ttggtatatg cgggagtcca tcctcgcatc agacgggaag
     8581 aagccatccg cgccttggac atggtgggcc ttgcggacag ggcatcccac agaccaaatg
     8641 agctttctgg aggccaacag cagagggtcg ccatagcccg tgccctggta agcaatcccc
     8701 gcgtagttct ggcagacgaa cccactggaa atctggacac caaatcgagc tacgagatca
     8761 tggacatcct tgccgacatg aacaagcggg ggataactgt ggtaatcgtt acccatgatc
     8821 ctgacattgc agcctatacc cctagagtgc tgacgttcag ggacggcgag tgcattgagg
     8881 atgttcgcgc tttggagggg gtgtagacaa gtgaggcctg ccgactatct gagactcgcc
     8941 ttgagtgggg ttacaagcaa tccgtttcgt tcaatactga cggctctcgg cgtgataatc
     9001 ggtgtatcgg ctgtgatcat caccgtatcg ataggcactg gggctaaggt gcagacgcag
     9061 tcgcaaatac agagactagg ttcgaatctg ataaccataa gcagcggata tagaagcttg
     9121 agatctgtta ttacctccga tattctgccg atcattgaaa gcatcaacac cgttggcgag
     9181 gtagtgccac aggtgcaaag gcagagcaca gtagtgcacg gagcagaagg tctcagcaca
     9241 accgtaactg gcacctcagc aaactatcct tcggtaagga acctgaagtt agcgcaaggc
     9301 tcctttttga ctgatgcgga tatcgacaaa ggttcatgga attgcgttct cggggcggat
     9361 ctggcggaag aactcttcgg agatgataat cccctgggtc agaaagtccg gcttgacaga
     9421 gtgcgtttca cggtgatagg cgtcgcgcaa cgccagggcg acagcggatt cgcaagttcg
     9481 gataacgcca tgtatgtgcc gtatacgacg atgcagcgtc gtataaccgg gagcaagaac
     9541 ataaacacaa tcattgccca agcagtagac gcttcatcaa tggatcttgc ctacagtcag
     9601 atatacgcca tgctcatgat ggctaccggc gacgaagact cattcaggat atctaaccaa
     9661 gccgacatgc tggaagtagc atctgatgtg actgacacca tgacattgct gttgtcaggc
     9721 attgcagcgg tgtcgctggt cgtggggggt atcgggatca tgaacataat gctagtttca
     9781 gtgactgaac ggactcgcga gataggcata cgcaaggcta ttggagcaaa ggagcatgag
     9841 atcttgtccc agttcatgtt ggaggccgct gctttgagtc ttgccggggg ccttcttgga
     9901 atcgttgtcg gtttggtcgg gtcccgcatc gtgagccaag tgttaagctg gcccactgcg
     9961 gtggacatga aatcagtggc gttgggattt actctgtcat tgatagtggg agtgttcttt
    10021 ggggcatacc ctgcatacaa gggggcaaag ctcgatccca tcgtggggtt gagatacgaa
    10081 tgataaggaa tgtgagggat ggcgcatgag ggctagcgtt ggcagaagaa tgccgatttt
    10141 catcatcatg ctgattctgg cggcctcttc tggggcaacg gctgacggga cattcggggc
    10201 acgctggtca atcgacacgt ttgcggatgc tgctgtcaac gtctggaaga acagtccgca
    10261 agctcgacag ccgctggaac ttgcgctgat tggcgaggga ctgagcgtgc ccgttcgttc
    10321 agcatccata accatctcca gcggaatgtc gagcgctagt cagttaactg aatccacagg
    10381 agaccgcggc gatgctgaga aggtttccgc caaactcccg ataaccgaca ggatcagctt
    10441 gaacgcagct tacgatttga acaagtcttc gggttctctc aagctatcat atacccttcc
    10501 acacacttgg cttgcgggtt cggatgaagg tatcgctcag cgactgtttg gccattcggg
    10561 agccagtgag gacgccaagg tatcgacgga tatggcaagg atgaagctgg atgcatcaga
    10621 gtcgtcaata aagctcaagg cgcagaaagc ctatattggc gcgctgaagt ccattaagaa
    10681 tcgggagacg gctgaagagg agtggcggct tgcccagact gcacttgaga tagcaacgaa
    10741 aagatgccag gttgggctct ctacggaaag agacgtggag caagcagagc tttctgtact
    10801 cgatgctgaa atcgccctgg tgaaggcgga aaacgaggag cggtgggcta ggagagaact
    10861 gagcaagctg gccggtgtcg acatgttaga ggcagagctg gaagatcttc cgcagttttc
    10921 gttggacatg ccttcgcttg atgtgatgat cgaggcagcg ttcgagagcg atgtcgagct
    10981 tgctcaggca gaattgggcc tagattcgtc catccgggaa cttgaagcaa tcaaaagtct
    11041 gcttcctacg gtgtctgcag aggttcaatt tccgtcaggg agcagcgttc ccaagtttgt
    11101 agcgtcggcc acatggagca tctcgctttc gcgctctctc gaagcacaga aggcagagat
    11161 caatgtggat caaaagaggc agtcggtaac tgaaaggcgt gaggcattgg cagagaatgt
    11221 ggagaaaagc cttgagcaat tacatatttc ggtatggtcg gcagccaaat gggtgaagca
    11281 gcttgagcaa tcccaagagt cctatgatac agctttgaag tcgtataaag acggcgaatt
    11341 gctgttagtg gacctggagc gagcgcagct caatttgcaa aaagctcgca atagctatat
    11401 tgcaaactgg tatggtgtgt gggaactctg gtacacgctg gccaatacgt gccgggttga
    11461 agacctgggc atggggaacc gatagtcatc aaaaacgatc cggaggtgtg ttgggtgaaa
    11521 cgaaattgca tgttgaaaat ggtacccgtc ttgctggcag taacgttgat gttaagcggc
    11581 attgcgctcg cgggcgaata tgtagatgaa acactggagg tgactttggc tcagtatgtc
    11641 gaggcatatg tgggaaacag cgatgcagtg aaggcagctc gggaagatgc tgagaatgcg
    11701 cagacccaat ggcagcgggc tgttgatgag aagaaggctg cattgactgt gaaagagctt
    11761 gagaatgctg ccgagactaa gcgactcgct ttggatgagg cggtgaacaa tgcagctttg
    11821 caggctgttc aggcctatgt aggactcgga caggcactaa gggagctgga ggccaaacgc
    11881 gtttctttgg ctgtgtcaca ggaaaggcta cgagttacga atttgcggta tgaagcaggg
    11941 cttactacca aagatgccgt attagaacag gaaaactcgt atctttcagc ttctgattcg
    12001 cttttaaagg cagagcagag cctgaggcag acgaaacgga gcttctgcca gaacatcgga
    12061 gctgaacttt gtgatgatat tgtgctggcc actgacgtta gtacattata tggcggcgta
    12121 cccgagtacg gccttaacga ctccatcgag gctgcgcagg gagttggttc ctcatacttt
    12181 tcagcggccg caggcaagga attggcaaga aggaagtgtg aggcgcttca agatccgctg
    12241 attgctacgc ctacggagcg tgagacagcg gaaaagtcat ggaaggacgc cgagacccga
    12301 tttgccagcg ccgagcagtc tttgatagat tccatagaaa atgctctggt ggagctggaa
    12361 tcgctggtca gaaacttgaa catgcagacc atctccgcgc agcgcgctca gagcaaccta
    12421 gaggttgcca gaatcaagtt tcaacacgga gagatgctcc agtatgaact cgataacgag
    12481 acaagctcag ctaatcaggc actatacaga atggccaaag caaaggaaga cctgtttgta
    12541 catatgttga agttggaatc gctcataggc ggcagcgtcc tggacatcgt gatgagcaag
    12601 aaatgaggcg ggattcgtga ccaaagttct tctggcggat gatgaggcaa gaatgcgcag
    12661 gctcgtatcg gattttctca aacgggaggg ctttgtcatc cttgaagctg agaatggccg
    12721 ggaggctttg gatcttttcc tcagcgacca agacatagag ctcgtgatat tggacgtaat
    12781 gatgccgggc atggatggtt gggtggtctg ccgcgagata cgcaggagat cacaggtgcc
    12841 gataataatg cttacagcaa gagccgaaga gagtgatcaa ctcttcggct tcgatattgg
    12901 ggctgatgag tacattacaa aacccttcag ccccatgata ctcgtagcta gggctaaagc
    12961 cttgctcaga cgcacgcagg cgggcgatgg aggactcagg catcttggat tgctggagat
    13021 agatgaaggc aggcacgtgg tgagcgtgga cggtgaaaga ctggagctga gccccaagga
    13081 attcgagctt ctggtctact ttgccgacaa tgccaaccta gccttgtcgc gggaccagat
    13141 actcagctct gtgtgggact acgactattt cggggatgcc aggacagtgg atactcatgt
    13201 gaaacgtctt cgggccaagc tgggccctgc agcaggctat attgagactg tacgtggcat
    13261 aggctacaga tttgaggcga ctgaagaatg aagtctataa gagcacgctt attcctggga
    13321 atcgttggcc tggtcgtagg ctacgtatta ctctcctggt tgctcaactc gcagttgctg
    13381 gatgtgtact atcaccgtat caagaccaat tcactcgtaa gcggctacaa gcatgtgaag
    13441 caggcatacg atagtggcga cttggcccag ttgttgctgg atctggagac aatggagcgc
    13501 aaggagggcc tgatggtggt tatagtggat tcctcctaca cgctccagta cgcgtcgagg
    13561 cctgtccaat ccgccaggca cggctctgcg ggtccgacgg gccctatgga ttctgcacta
    13621 ctcgattcgc tcgatcagca aagaatgccg ccgccaggcc ctgggcctaa tcccgctcca
    13681 aacccggacc cgttccccgt aaatacgatc gaacgcgcga tagctgatct agagggtgaa
    13741 tacgaagtcc tcagtttcac ccgcgacgac aggcttaagc tggacttcat aagcctaatt
    13801 ggaaggctgc ctgatggggg ttatgtattc ctcagcgcac cgatctcgtc gattgaggag
    13861 agtgcatcgg cagctaatag gttctttttg gtaagcggag tattgatgat agcagtgggg
    13921 agtatcgctg cctattggta cgccggacgg tttacacaac ctatccatga catgaaagtc
    13981 attgctgagc gcgtggcaaa gctcgatttc agcatggagt atgtcggcag caccgatgat
    14041 gagatcggcg ctctcggcga gagcatcaac atcatgtcgc gccagctgga gtactccata
    14101 aacgaactgg tgcgcgccaa tgaacgcttg cagcaggaca ttgagcgcga acgtcagatc
    14161 gacgaaatgc gcaaggaatt catcgcaaat gtgtcgcacg agctgaaaac gcccattgcg
    14221 ttgattcagg gatattctga aggactaaag ctcaatgtca acgatgacga agagaatagg
    14281 gatttttact gcgatgtaat aacggacgaa gctgccagga tgaatcgtct tgtaggccag
    14341 ctgcttaaac tggcccagat tgaatcgggg aatattgcac ctcgaaaaat ctgcttcgac
    14401 atcgttgagt tcatcaacca tatcgtcaga aaaaacggtt tggcgttgca agaacgagga
    14461 gttgacttgg ggatagaggg cgaatcgtgt gtggaggttg tggccgacat cgacatgatg
    14521 gagcaggtgt tggtcaatta cattaccaac ggcatcgacc attgcgacgg ggagcgcaag
    14581 atgaccatca caatagagga ggtcgatgag aaagcgcgga tcagtgtttt caatacggga
    14641 caacacatac ctgaggattg catggattca atatggacta gtttctataa gactgacaag
    14701 gcgcgcacac gtgaccatgg cgggacaggg cttgggctga gcatagtgaa agcagtgcaa
    14761 aaggctcacg gcaatgattg cggatgtaag aacgttgcgg gcggagtagt attttggttc
    14821 gatgccgacc tgtgttgact gtggagtaca ggtgttgcgt ccattatcac tccgaacagt
    14881 ttcaaggaga agcgagattt gagctaccaa tgatgatggc actcacataa agcaatcatg
    14941 accgggtaca ggatacgggc ggatgagagt tgaatcgcgt gatgccgaat cggggtgtga
    15001 tcatatctgc cagccgctgc cgtagcgttc tacacattcc tccctggcaa tgggattttg
    15061 cgctgggctt gtgcagtaat acggaagcag gctcattcct taatgcttct tgaagcggct
    15121 tcattgcgac gagaagaggc cgatagataa gttcacaaac aggccagttc ccttctttca
    15181 ctatagaatc atctgtcaag attggcaact cctatcgagt ctttagacct taatagaccg
    15241 ccgcctctgt ctagcttttt atttgaggtc tgcatcccct gcttaccctg tagggcttgg
    15301 aatgggatgc attttctcag tgaggcggcg aacaggctaa cgggtggatc cggtttcgat
    15361 tatctgactt ggctttcaaa gatctgggcc ttgtcaagtc tcttcgtgat ggccatcaag
    15421 agggcgtagt cgacgatgat gagagctgct ccgatggcca gcagcacttt tgcatcgagc
    15481 agcatgatgc cggtgaactg cccgatcatc agggcaatca ctggcagaac gagaaaaccg
    15541 ctcatttgat acccagcctg aaacgtagat acgcgcgcgc tgatgaagac gtttatgagc
    15601 acggcgagaa gcccaattgc cggcattacc aaaaggatga ggggagccca ggcgatgcta
    15661 gggaagaata gtctgccaaa tatgggccag gcgaagacgt tcgcgcatac cgcgaaaagc
    15721 acgaaggaag tgagggaaat ggcgatagcg ggaataagtg atgccaaagc ctttgccgca
    15781 aatatgctcc gcatgtcgac tggagacaga agaagccctt ccagggtgcc cctttccttt
    15841 tcacccacga agctgtcgac actgattgtg ctggcggcca tgagcggtat catcaggaaa
    15901 aacggggcca tgaaatagtt gaacatcatg taggcgattc gctgctgatc ggtgatgagt
    15961 gagtcgaata tcccattgag ggagtcaagc gacgactggg gcagcttttc aagcacgtcg
    16021 agcagctcct gaaaatcact ggaggtgctg gtgtcggtcg cccttatggc gaaggatgcc
    16081 ccaataggga acaagacagc aaagacgaga gggactatta gcatgggcag ccacacttga
    16141 atgttggaag tgatgctcat gatatctttc cgggcaatgg cccgcacagc gcgcctattc
    16201 atctttctct ccctccaata gcctgaaata gactgattct aggtcatggc ccttcaactt
    16261 aaccgagtag acgtctgcgt cttgcgagag ttgacggatc aacttaggaa cttcattcct
    16321 gcttgaaacg gtaaattgaa tgtcgccgac cggcagcggt cttgctggaa tgccccggta
    16381 ggcttctcca tccagaatgt aatcggcatc tacttccaca atggtgggcc cgggacattt
    16441 ctccgccagc tcgggcaggg ttccctgggc cacagtgcgg ccccggtcga tgatcacgta
    16501 gcgttggcat accgtttcca gctggtgcag gacgtgggag cacaggacta ccgtcacgcc
    16561 tttctggctg ttcaggcgct cgatctcatc gagaaccatg gcgatccccg ccgggtccag
    16621 gcctgatgtg ggttcgtcca gaaggagtat cggcggattg gcgagcaagg ccttggcaag
    16681 ccctagacgt ttcttcatcc cagtgctgta ggcaccggcc tttttttctt tgtgatccgc
    16741 gagcccgaat tcttccatca gctcatgcgg tcttcgggga tcgtctattc catacaactc
    16801 cgcaaagaat cggaggtttt cgatcccgga gaggttggaa tagagctcgg cagtctcggt
    16861 gaggacgccg atttgggaac gaacttcatc cccctggctt tggggatcaa gacctgcaac
    16921 cctgacttgg cctgcatcag gctttacaac gccgcacagc atccggattg tggtggtttt
    16981 gcctgcgccg ttagggccga tgaggcccag aatctctcca cgagccacgt tgaaactgag
    17041 ggcgtcaacg gcgcgttgct ctccatatcg tttggttacg tccttgatct gaatcatcat
    17101 gccacacctt cacattgatc tagtagccga tttctgtatt cgatagctcg cgttcgtttc
    17161 aaagtgggag gagatgccta aaagagttac gctacttctc cgctttgatt gatccacagt
    17221 aaggacagat ctccaattcc cgggggatcg gcttgctgca tccggcgcat cggacgttgt
    17281 tgcgcctggt aaccaggatg tagacgatta tggaagacgg gatgttgatc agcccgaata
    17341 aaccccacag ccacgcatag tcttcacggc gtaggcgcgc gtcacggaat atccatgtag
    17401 cttgcgcaat gagcagcgcg cccagaaaga tccagagaat cggatcgagt tcattcacgc
    17461 tacatcacct cttccctctc gcaacacgtg gagaggcagg tagaaaaggg ctatgccgaa
    17521 gaatccgatt gcctgaaggc ccaggaacgc accgggcttt gaggccagaa gcggaaccca
    17581 cacggaagtg agaatacccg tggcagtcag cgcgaacagt gccatattcc gacgttcccg
    17641 ggcaattcgc atcttgattc cccgttcgat ggtcgcatcc agcctcggcc aagcgggggc
    17701 gatgccttca attcctaggt ccgtcgtggc gggacgctca gcgcttccag aaggtacatt
    17761 cacatgcgat gatggcagca acgacgcgat atctacgagg gcgtcagcgt agtctcgaca
    17821 ctcggggcag tttttgaggt gtctggccac atcggtcgtg cttatgtctt tgtcaagcct
    17881 accttggtcg tcggcctcgc cggactcagg gtacgccatg tcgagcagca tatcttgaaa
    17941 gttcctgcaa tctatgttca cttcatgtcc ctcctggaga tttcacgcct gagtatttca
    18001 acggaatcat gcagccggga ccgtactgtc cctattgggc agccaaccgc gtcggctatc
    18061 tcctcatatt tgaacccgta gtagtgtctt aagacgaaca cttgtcgttt ttcgggcccg
    18121 agtgcatcga ggaggctcat cgcctcgtct acagccatgt tccgaagagc cttctcctcc
    18181 gcgctctctt cctcggaacc ggtttcgaag acgtcctcag tcaaaatctc gcgccgcgcg
    18241 gtcctaagct tgttcttgtg ggtgttcagg gctatggtga tcagccatgt ggagaacttc
    18301 gctcgcggct cgtagcgttc taggttgacg atggcgcgga gcaccgtctc ctgtgctatg
    18361 tcttctgcgg atcggggatc gcccgtgatg cggatcaatg caccaaggag aattgggtag
    18421 ttgatccaga agagctcttc caaagcttgc ctgtcgtttt gttgcgcgcg gcgtactagc
    18481 gaagtctcgt cgggcatctc cagtcccctt ttccgttaat tgaacacagc tggcccgcaa
    18541 atagttcggt cgccatgaag gacgatatca gactatatca taccgtgtgt ggcaaatggc
    18601 attgaatgat tgcccaccaa atcgcgcatt ctcttttcgt tgttgcaagt cctgtaagtg
    18661 tttggccatg ccagaaaaac ttccgctgaa ttg
//
LOCUS       DAOSYL010000047        18073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2563_length_18073_cov_8.770452, whole genome shotgun sequence.
ACCESSION   DAOSYL010000047 DAOSYL010000000
VERSION     DAOSYL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18073
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_18073_cov_8.770452"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            657..1199
                     /locus_tag="PLU15_08000"
     CDS             657..1199
                     /locus_tag="PLU15_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOO30904.1"
                     /translation="MKIGPRIKNLRLKKGLTQEELGERTDLTKGYISQIERDLSSPSL
                     ETFFDILEVLGCTPKEFFSDEEREQKVVYTDEDLTEFVDDERGYRIQWLVPESNENEM
                     EPIMLTLEKGGSFKQFEPSLADTFAYVLSGRIKVSLGKRTYYAKAGQTVYYQASEEHQ
                     ISNDNDGPSELLLVATDSYL"
     gene            1259..2365
                     /locus_tag="PLU15_08005"
     CDS             1259..2365
                     /locus_tag="PLU15_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30905.1"
                     /translation="MAKNTIIRFKNVTKQYDDDPPVLTDVSFELERGKFYTLLGPSGC
                     GKTTIIRLIAGFVEPTVGEIYFDDQEISKIPPDKRRVNTIFQDYALFPHLNVFENVAF
                     GLRVKRMKEDVIREKVREALRFVNLEGLENRDIAGMSGGQQQRVAIARAIVNEPEVLL
                     LDEPLSALDLKLRTEMQYELRELQRSLGITFVFVTHDQEEALALSDEVFVLNNGRIEQ
                     RGTPMDIYDEPVNRFVADFIGESNIISATMIKDYLVEFVGNQFECVDKGFHPNEPAEV
                     VIRPEDLEITPKDQGKLQVYVDSQLFRGVHYEICCYDKEGNEWLVHSTKKVAVGDEIG
                     LNFGPEDIHVMRLGETEDEFDRRLESYEEVRHAG"
     gene            2355..3155
                     /locus_tag="PLU15_08010"
     CDS             2355..3155
                     /locus_tag="PLU15_08010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017691799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30906.1"
                     /translation="MQAKARNIYLIPYMLWIIMFVIAPIVLIVYYSMYDIDGVWTLAN
                     YKRFFTPVYLRMTLRSFWYAILITGVSLIVAYPTAYLLTKTAHKELWLLLIILPSWIN
                     LLLKVYAFIGILGLYGPINSFLELVGIGAKQILFTDFSFIFVAVYIFIPYMILPIFNA
                     LEELNPSLVAAARDLGASPWATFRRVVLPLTMGGVKSGCRAVFIPALSLFMITRLIAG
                     NRVITLGTAIEQHFLVTQDWGMGSTLAVFLIIVMGLTMLVTESGSRVK"
     gene            3157..3960
                     /locus_tag="PLU15_08015"
     CDS             3157..3960
                     /locus_tag="PLU15_08015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO30907.1"
                     /translation="MKKTGKLSVIYLVLVFIILYTPIFYLIYYSFNSGGSMHHFQEFT
                     LDWYEEVFHDTRLFIILLNTLVVALLSSAISTIIGVAGALAITRMRSKRTRNALLTLN
                     NVMIVSPDVIIGASFLILLTAIGVNLGFFSVLISHIAFSVPIVVLMVLPKLEEMSPTL
                     VDAARDLGASSRDVLTKVVIPYISPGIFAGFFMALTYSLDDFAVTFFVTGGGFSTLSV
                     EIYSLARRGISLTINALSTLIFLATLALVIGYYIITKRGSKPSGAEARE"
     gene            3957..>4981
                     /locus_tag="PLU15_08020"
     CDS             3957..>4981
                     /locus_tag="PLU15_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30908.1"
                     /translation="MKKVTQMFTIVFAAAFALMYLTSYLNALHGYSGPNTLTIYNWGD
                     YIDPYLIKEFERETGLKVIYQTFDSNEAMLTKIAHGGVTFDVVIPSDYAISKMKEQEL
                     LIPLDHSKLPNLKHIDARFLDLPFDEKNVYSIPYFWGTYGIIYNPEVITDIDFDSWDD
                     LWDPRLQNQILLVDSAREVIGMGLNSLCYSLNDTDMDHLQQAKAKLDMLIPNIRAIVG
                     DEIKMLLETEEAAVAVVWSGDASEIIGENDKFDYVIPKEGSNVWFDNMVIPTTAKNVE
                     GAHQFINFMLDPENAAQNAEYVGYSTPNAEALRYLPEEITSDERFYPGSEITDKLEVY
                     ENLSKRMT"
     assembly_gap    4982..5081
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5082..5896)
                     /locus_tag="PLU15_08025"
     CDS             complement(<5082..5896)
                     /locus_tag="PLU15_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30909.1"
                     /translation="MRIKKTVLALVLVIALIPAAAAFAEDDPVGTAAMMGLGMGARAL
                     GMGGAHIAVADDASIIYYNPAGLGVVDGISVTSLYTNQYGAAGYLALGGVARHIGAGI
                     LRLDASGIEETDEFANEIGTFGVASTAVMAGYGMTVMPNLSVGAAVKYYQETLPENSG
                     KGFTADMGVLYSMADDKIRVGAVGRNLFGKVAYSSGAEDAFDRAFGLGVAFEPMENLL
                     VAADAVMDNGFSGKVGAEYRFRQMALRAGGSFGGNGTCLTAGAGFEAATFNID"
     gene            complement(5972..15220)
                     /locus_tag="PLU15_08030"
     CDS             complement(5972..15220)
                     /locus_tag="PLU15_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO30910.1"
                     /translation="MADSRRNRSMFIAALILLLVIPCAAVEGASDVWSQAGLCGGWQP
                     GPDTYHPNKVIVRFSDTITTNAATNSIERLGYSMYRVADFKATATFPSGAKFGIVELP
                     ERVSPESAISRLSNAPGILYAERDYICYKDQAHTDAPIVPNDTYFERMWGLHNENCQY
                     KDPEMSGDPVNNADIDAPEAWAVHTGTDEIIVAVIDSGCYIQHPDLIANIWVNESEMN
                     GEPGVDDDGNGYVDDFWGWDFANNDNSVFDPDERDEYGYLNDNHGTHCAGTIGAQSDN
                     SMGVTGINWNVRIMPLKFIGPGGGYTSDAILALQYAADKGAQIASCSWGGGPYQQVLK
                     DAIEASGMLVVCAAGNSGDNTDIHPHYPSSYDSANIISVAAMMQNEMPCNYAGWWSTC
                     YGERTVDLFAPGGYILSTVPPDPPEAEPSEAYAFFYGTSMATPHVSGAAALLRSIYPH
                     VPLYRTAGMGDDTVTIKDMILGSVDVFPQYEGKVLTGGRLNLANAIAGQRAPIITSIE
                     ATPTWGPPPLEVSFSAMAETPEGEILDMWWDFGDGSETVHEWDAVHTYEEEGFYDASF
                     HVVNTAGIDASAAVQIHVFFASVIGVEPTEIHTRLEWGETCEEAITISNDGLGSLDYT
                     AELQLIGMVDTSGTAIPIGKLGSGGPDEHGYFWTDSDEAGVVMPEWFEISEVGTGVTL
                     EGEDSVVVNLPFEFPFYGDIKDSIRISSNGYLTFGDSGTAYNNHSVPDTREPNDLIAI
                     FWDDLEPQKGDGKVFYYGDDQKFVVQYQDVQRYGRGGPYTFQATLTPGGNITYLYKMM
                     GTRLDEATIGIENATGTIGLQVAYDENYVHDNLGVLFVPGWVVLDKTAGTVAPGESDT
                     LTAAFAAHNLPQGTWKATIKILSNDPVNPQVDVDTFMFAKSIIPPVIRSISADPRIGS
                     APLAVEFSAVAEDADGEIAAIEWDFGDGSHPVTGVFAPVHTYEADGEYTATFTVTDDD
                     GLIAEESVTIVVADLPEASVDPQTFHQAVRAHRQKTETLTVTNMGEAALIFSAEAFTG
                     GVPTEDGALSTLGVGGPDGFGYIWRDSDASGGPVFDWVEIGEIGTKLPLTGDDSRVVD
                     LPFDFPFYGDTKTSIRICSDGYLTFGTKGNAWANKPIPTSDEPNDMMAVYWDDLNPPQ
                     APTDGGVFYYHDTVNNCFIVEWNKVPRYPESGEYTFQAILYPDGKIVYQYLDMRFISS
                     YKGKGTIGIENATGTDGLEVLNNREGYMRNGLAIEIKPVSWMSIAPAQGEVAPGESID
                     LDVTFDLGMVRSGTRDGAVVLHTNDVRKPRTIVPVNIQIIPNSPPEIRACAVNPQQGP
                     PGTSFQFVGAAHDPDGSIVDKHWSFGDGSPVVHEFVASHVYTAAGVYTATFTAVDNDG
                     YITTVTMKVTVAEAASASWHPGQLNFTIGVGQTATGTVTLSNAGPGALHFGNGEFPSL
                     AQMPERLVLPEDIKDAQARTALGVYGAHPNPERSEWLPDTVGSVITRWRCPSPISSGW
                     GVGVLFDTQNLVISDPDSTPTADYIVTPEGAYTGTSWAANFGGSWAGDLAFDGQHIWQ
                     VNVGGDNAIYKLDPANGAVVGSISGPGWTSISQRGLAYNRNDDTFYIGGWDEDIIYKI
                     KGESWNTPGQIVEQWSLPVGIAGLAYHPVADILVATANSSPDMIYLIHPGSHAVIAQF
                     THPYNGDYSGAGCEFGPDGNLWVASQANNTMYLVETGLGPIGAGDWLTWEPEEGSVPA
                     GGTAPISITVNSERLGPGRHEANVVLGTNDIENPLIIVPVALEVAAPPVITRATAEPT
                     FGEPPLEVAFNAEFDAPEVPIASYGWDFGDGTSSSELDVTHSYTQPGKYIATFSVVDQ
                     LGVEDKASFEVEAKWLPRATCEPETIEVTLSPRGSVTKTVALGNVEGNADLEFKVRVK
                     SGGAPTIAMPERVGIAMDENARTAEGLYASIAPELVEKIAASIEPGAVGDVITSWPVP
                     SQIDMPWGVGFDGNLWISDAQAKKDHVVTPEGAHTGLIFRTPWAGSWVADMAYDANRD
                     LMWQVNVGGDNGIYGLDPETGAVVMKITDGPWTYSSQRGLAYRAEDDTFFIGGWDEDI
                     IYHINGPSHESPGSVIGAYKFPVGIAGLAWHPDGILWVSNNGSPDMIFGLDLDALEVV
                     HQFPHPFGGEYHGGGLALNSDGNLWVVSIDNSRVYLVSTEMPWAPGIAMSSIDGTVAM
                     GETEELDVTINAEALGKPGDDVRRCLRIRTNDPENAILYVDLIVHIEAGPTIAEATAT
                     PTIGEPPLKVAFNAVTEPGATDIIDTWWDFGDGSDLVHEAATEHVYTDVGAYKATFHA
                     IDENEVEAIAELAVTVKWLPVLGVEPGEINAIIQAGEEEQAVLTVSNTGVAPMNFEIS
                     IVPDLPKDPNGEPAGCGYIWMDSNHDGGPAFDWFEISDVGTGVKLYDESFVDVDLPFP
                     FPFYGEMKTEVRICSNGYLTFGMTRSEWTNTPIPDSSDPNDLIAIFWDDLNPGSGGNV
                     YYYHDQEAGSFIVEYQAVPGRTDSGDYTFQAILYPDGTIIFQYLEMTADVTGATVGIE
                     NATGTDGLQVVYNAPYIEDGLAIIFAPIESILRVNPSSGYIVPGGSQEVQLTLGSPDA
                     AYGTYSLRLCVAADNPLYPFATVPVTIKVNAAPSVAITAPVGGDELHGKYEITWVATD
                     HDDDEDELLIDLAWTRDGETWHELGTGLANTGSFEWNTIEVGEAGETFLVRARATDPA
                     GAFHEFVTDEFTIVNLAPNADFRFTPSPATRRDKVKFVDESTDDGEIVAWHWEFGDGA
                     ESEQESPEHQYTQKGEFEITLIVTDNGGLTGSATKTIQVGNAKPIAAFSFTPSPARVG
                     EVVRFTNESTDDGDIVACFWEFGDGATSAQCSPEYVYSSTGTFAVKLTVIDDDNVASS
                     ITREIEIINLPPEVEIVRPTAGQVLTGEAMIEWEAFDPDDDADTLKITLEYEPASGGD
                     WQAIASEAPNTGKYIWDTSKLERGGKYRIRITAVDPEGASGDATSEEFTVIALTRAVV
                     AAPNPTSDLVTFYYDIASDGTLYVYDIAGRLVHSAELSATANAHEWNLHSSGKPVANG
                     VYLYVVIAGEERSEVGRLVVSR"
     gene            complement(15830..15988)
                     /locus_tag="PLU15_08035"
     CDS             complement(15830..15988)
                     /locus_tag="PLU15_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO30911.1"
                     /translation="MVLPQCLRKRLRTTKRLNEEIRRREWVMRIFPNDDSGSSPKRAV
                     KTVLPRGC"
     gene            complement(16042..16155)
                     /locus_tag="PLU15_08040"
                     /pseudo
     CDS             complement(16042..16155)
                     /locus_tag="PLU15_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010924694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16110..16253)
                     /locus_tag="PLU15_08045"
     CDS             complement(16110..16253)
                     /locus_tag="PLU15_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30912.1"
                     /translation="MVDAIVIKIRKGGRVWNHSALIAVGVALGSCLQIVRRKTRGATS
                     SSL"
     gene            complement(16246..16488)
                     /locus_tag="PLU15_08050"
     CDS             complement(16246..16488)
                     /locus_tag="PLU15_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO30913.1"
                     /translation="MNSHIFTAIASYLPSNMFIALIATLVEMVVDGVSTRKVCKITRE
                     LCGAEVSKSTVSAMVTCSARMARAFSRQRAISISNG"
     gene            complement(16713..17855)
                     /locus_tag="PLU15_08055"
     CDS             complement(16713..17855)
                     /locus_tag="PLU15_08055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO30914.1"
                     /translation="MKVNLARPDITQAEIDAVLDVLNSYTLALGPRMMEFEMAIASYC
                     GRKHGIAVNSGTSGLHLLVRAYDIGQGDEVITTPFSFIASSNCILYEGAKPVFVDIEP
                     DTANIDPDLIEAAITPKTKAILAVDAFGQPAKVDVIREIARRNDLVFIEDSCEAIGSH
                     YKGKPAGGADFADAAVFAFYPNKQITTGEGGMVVTDDDRIAFLCSSMRNQGRGESGVW
                     LAHERLGYNYRMDEMSAAIGVVQMQRIEEIIEKRAAVAKMYKERLSGITGVRLPHIAQ
                     EVTRMSWFVYVIRVGWDEETPKMQSKVRDHVMARLGEADIGCRPYFTPIHLQPFYREM
                     FGYQEGDFPVTEELGRTSIAIPFYNNLTEDEIDYVGQVIERALEER"
BASE COUNT         3933 a         5062 c         4625 g         4353 t
ORIGIN      
        1 cgcggcagaa gtaagccctg tgagtcttgc tatctctcgc ctcgtgatgg gctggttcgt
       61 ccgtatgacg tccagcacaa gctgtctgtt gtggataccc acctgaaccg agtttctgcc
      121 cgactgttct tgcctcacat tcccacaatc cttttactaa gactttggct ttcagtttga
      181 taaagtagtt ctccatctgc ttggaaactc ctcctccttt ctccagaaag ttttgtgccg
      241 cgcccatttg tgtgtttggc tcgaggtaaa cagatgcaat tcactcggca gtcttgtatg
      301 tcggggcaga catactcatg atgacgagaa catttcgcag gagcgtaccc gcgtatgagt
      361 ccagttgggt atttaataag ccatctagaa ggcttccaca gccagccgag cctcggacca
      421 cagacgtcgt acatcaacct ttaagtgtta tctaaggatt gtcatatgtg ctatgcccaa
      481 aatcagtagt gacattatca cagacgcgta acacgcgtcc gaaattggca ttgcactttt
      541 gcaggaatcg tatataatat cttagcgtcg gcagtaaact tggagtttac ctgtgttgaa
      601 cttttcaact tgaagtttac ccggcctaaa cccaacagat aagcgaggag cgacggatga
      661 agatagggcc aagaatcaag aaccttcggc tgaaaaaagg tctaacccag gaagagctgg
      721 gcgaacgcac tgatttaacc aaaggctaca tatcccaaat cgaacgcgac ctgagctcgc
      781 cgtcgctgga aaccttcttc gacattcttg aagtgctcgg ctgcacgccc aaggaattct
      841 tcagcgacga agaacgtgag caaaaggtcg tgtacacaga tgaggacctg accgaattcg
      901 tcgacgatga acggggttac cgcatccagt ggctggtccc tgaatcaaat gaaaacgaga
      961 tggagccgat tatgctcacc ctcgaaaaag gcggaagctt caaacagttc gagccttccc
     1021 ttgccgacac ttttgcctac gtgctgagcg gaaggatcaa agtttcgctc ggaaaacgaa
     1081 cttactatgc taaggcaggc caaaccgtct actaccaagc atctgaagag caccagattt
     1141 ccaacgacaa tgacggacca tcagaattgc ttctggtggc aactgattcc tatttatgat
     1201 gtaaagagtt gtgcatcgtg catgatatcc tacttccatc cgcggggagg ggacaagaat
     1261 ggccaaaaac acaatcatcc gtttcaagaa cgtcaccaaa caatacgacg atgacccccc
     1321 ggtgttgaca gatgtcagct ttgaactgga gcggggcaaa ttctacacat tgctcggccc
     1381 ttccggctgc ggaaaaacga cgatcatccg cttaatcgca ggcttcgttg agcccacagt
     1441 cggcgagatc tactttgacg accaggagat aagcaagatt ccgcctgaca agcgccgggt
     1501 aaacaccata ttccaggact atgcattgtt tcctcatttg aacgtgttcg agaacgtcgc
     1561 cttcggactg cgcgtcaaga ggatgaaaga agacgtgatt agggagaagg tccgggaagc
     1621 gctacgtttc gtcaatctgg aaggcttgga gaaccgggac attgcgggta tgtccggcgg
     1681 ccagcagcaa cgcgtggcca tcgcgcgagc catagtcaac gaaccggaag tgctgcttct
     1741 ggacgaaccg ctttcagccc ttgatctgaa gctccgcacg gaaatgcagt acgagctgag
     1801 agagcttcag cgcagtctgg gcattacatt cgtctttgtc acacatgacc aggaagaagc
     1861 ccttgcactt tccgatgagg tttttgtctt gaacaacggt cggattgagc agcgcggaac
     1921 gcccatggac atctatgatg agccggtcaa caggtttgtg gctgatttca taggcgaatc
     1981 caacattatt tcagccacga tgatcaaaga ctaccttgtc gaatttgtcg gcaatcagtt
     2041 tgaatgcgtg gacaagggat ttcatcctaa cgaaccggcc gaagtcgtga ttcgtcccga
     2101 ggacctggag atcacgccga aggatcaagg gaagcttcag gtttacgtgg attcccagct
     2161 cttccgcggc gtgcactacg agatttgctg ctacgacaag gaaggcaatg aatggcttgt
     2221 ccattccacc aaaaaagtag cggtaggcga tgagatcggg ctcaattttg gccccgaaga
     2281 catacacgtg atgaggttgg gcgagacgga agacgaattc gacagacgtt tggagtcgta
     2341 tgaagaggtg cgccatgcag gctaaagcgc gcaacatcta ccttatcccc tatatgctat
     2401 ggatcatcat gttcgtcatt gcgccgatcg tgctaattgt ctactactcc atgtacgaca
     2461 ttgacggagt atggactctc gcaaactaca agagattctt taccccggtg tacttgcgaa
     2521 tgacgctgag gtcgttttgg tacgccatct tgatcacggg agtctcgctg attgttgcat
     2581 atccgacggc atacttgctg accaagacag cacacaagga gttgtggctt cttctcatca
     2641 tattaccgtc atggataaac ttgcttctca aggtgtatgc cttcataggc atcttggggc
     2701 tctacggtcc catcaactcc tttctcgagc ttgtgggcat tggggcaaaa cagattctgt
     2761 tcacagattt cagcttcatc tttgtagctg tttacatctt catcccatac atgatactgc
     2821 ccatattcaa cgcccttgag gaactcaacc cgtccttggt tgcagcagcc cgcgacctcg
     2881 gagcatctcc ttgggcaaca ttccgtcggg tagtactccc cttgacgatg ggcggggtga
     2941 agtcgggctg tcgagcggtt ttcatcccgg ccttgtcgtt gttcatgata acgcgcctga
     3001 ttgccggaaa cagggtaatc actctgggga ccgccattga acaacatttc cttgttacgc
     3061 aggactgggg catgggttcg acccttgccg tctttctgat cattgtaatg ggactgacga
     3121 tgcttgttac agaaagtgga agtcgggtga agtagcatga agaaaacagg aaagctgtcc
     3181 gtcatctatc tggttttggt attcatcata ttgtacaccc ctatcttcta cctgatttac
     3241 tactcattta acagtggagg ttccatgcac cattttcagg aattcacgct tgactggtat
     3301 gaagaggtgt ttcacgatac acgcctcttc atcatccttc tgaatacact ggtagtcgcg
     3361 cttctctctt cagcgatatc gacgattata ggggtagcgg gggcacttgc catcacgcgg
     3421 atgaggagta aacggacccg caatgcgctg ctcactttga acaatgtgat gatagtcagc
     3481 ccggatgtga tcatcggagc gtctttcctg attctcctga cagcgatcgg tgtcaatcta
     3541 ggattcttct ccgttttgat ctctcatatt gccttcagcg tgcccatcgt cgtcctgatg
     3601 gtgctgccaa agctagagga gatgagtccg acattggtgg acgccgcccg ggatctagga
     3661 gcgagcagcc gggatgtgct aacgaaagtg gtgatcccct atatttcgcc tggcatattc
     3721 gcaggttttt tcatggcctt aacctactcc ctcgacgatt tcgcggtcac ctttttcgtg
     3781 acgggaggcg gcttttcaac gctgtcggtg gagatatact cgctggcaag gcggggaatc
     3841 tcgttgacca ttaatgcgct ctctacgctg attttcctgg ctaccttagc attggtcatc
     3901 ggttactaca tcataaccaa gcggggatcc aagcccagcg gagcggaggc acgggaatga
     3961 aaaaggttac tcagatgttc accatagtct ttgccgcagc attcgcgctg atgtacctaa
     4021 cttcatacct taatgccttg cacggctatt cgggcccaaa tacgctcacc atttataatt
     4081 ggggagatta tattgaccct tatttgatca aggaatttga acgtgagaca ggcctcaagg
     4141 tcatctatca gaccttcgat tccaatgagg cgatgttgac aaagattgcc cacggcggtg
     4201 tcacgttcga tgtagtgatt ccgtctgact atgccataag caagatgaag gaacaggaat
     4261 tgctcattcc cctggatcat tctaagcttc caaacttaaa gcacatcgat gctcgtttcc
     4321 tggatttgcc cttcgacgag aagaacgtgt attccatccc ctatttctgg ggaacgtacg
     4381 ggatcatcta taaccccgag gtaatcacgg acatagactt tgacagctgg gatgatctgt
     4441 gggatccacg cctccagaat cagatcttgc ttgtagacag tgcccgcgaa gtgatcggga
     4501 tgggcctgaa cagtctctgc tattcattga acgatactga tatggaccat ctgcagcagg
     4561 cgaaggcaaa acttgacatg ctcataccca atattcgagc aattgttggg gatgagatta
     4621 agatgcttct cgaaactgaa gaggccgcag ttgctgttgt ctggtcggga gacgcctcgg
     4681 agatcatcgg cgagaacgac aagttcgact acgtcatccc aaaggaaggg tcaaacgtat
     4741 ggtttgacaa catggtgatt ccgaccacgg cgaagaacgt ggagggagcg caccaattca
     4801 tcaacttcat gctagatcct gaaaatgctg cccagaacgc ggaatacgtc ggctactcga
     4861 caccaaacgc cgaggccttg aggtatctcc cagaagagat cacctccgat gagcgtttct
     4921 acccgggctc tgaaataacg gataagcttg aggtgtacga gaatctcagt aagaggatga
     4981 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaatcgatg ttgaaggtcg
     5101 ctgcttcaaa gcctgcccct gcagtgaggc atgttccatt cccgccaaaa gagccgcctg
     5161 cgcggagcgc catctggcgg aatctgtact ctgcccccac ctttccggaa aatccgttgt
     5221 ccatcacggc atccgccgca acgagcaagt tctccatggg ctcgaaggca actccaagtc
     5281 cgaacgcgcg atcgaaggca tcctccgcac cactggagta ggccaccttg ccgaagaggt
     5341 tccttccgac cgcgcccacg cggatcttgt catccgccat ggaatacaaa actcccatgt
     5401 cagccgtgaa gcctttccct gagttttcgg gcagtgtctc ttggtagtac ttgaccgccg
     5461 cgccgacgct caggttcggc attaccgtca tgccgtagcc tgccatcaca gcggtactag
     5521 ccacaccaaa tgtgccaatc tcgtttgcga attcatctgt ttcttctatc ccggaagcgt
     5581 cgagcctcag gattcccgcg ccgatgtgtc gtgccacgcc tcctaacgcg agataccctg
     5641 ctgcgccata ctggtttgtg tacagggatg tgacgcttat accatccact acacccaagc
     5701 ctgccgggtt gtagtatatg atggaggcgt catctgccac cgcaatatgg gcgccgccca
     5761 ttccaagcgc ccgcgccccc atgccaagtc ccatcatcgc cgccgttccc accggatcgt
     5821 cctcggcgaa tgcagctgct gcgggaatca gagcaattac gagcacgagc gcaagcactg
     5881 ttttcttgat tcgcattttc cgccctcctt aaggtcgtgc aaatctgtga agtctgggcg
     5941 gtcgcttgtg cgaccgccca gacccatgca tctatctgct cacaacaaga cgcccgacct
     6001 cggatctttc ttcaccggct atcacgacgt agaggtatac gccgtttgcc accggcttgc
     6061 ctgatgaatg gaggttccat tcatgggcat tcgccgtggc tgaaagctct gcagagtgca
     6121 cgaggcgtcc tgcgatgtcg tagacatata gcgttccgtc agatgcgatg tcgtagtaga
     6181 acgttaccag atcagaagtg gggttcggag ccgccactac agcgcgagtg agcgcgatca
     6241 ccgtgaactc ctcgctcgtg gcatctccgg acgcgccttc tgggtcaact gctgtgattc
     6301 ggattctgta cttccctccg cgctccagtt ttgaggtatc ccagatgtat tttccggtat
     6361 tgggcgcctc agatgctatg gcctgccaat ccccgcctga tgcaggctcg tattcaagag
     6421 tgatcttgag cgtatctgca tcgtcatcgg gatcgaaggc ctcccactcg atcatcgcct
     6481 cgcccgttag gacctggcct gccgtaggcc ttacgatctc gacttccggc ggaaggttga
     6541 tgatctctat ttcgcgcgtt atcgagctag ctacattgtc gtcatctatc actgtcagct
     6601 taaccgcgaa cgtgcctgta gagctgtata cgtactcagg gctgcactgg gcactcgtgg
     6661 ccccatcgcc gaactcccag aaacatgcga ctatatcacc atcatcagta gattcgttgg
     6721 taaacctcac aacttcgcca actcttgctg gcgatggggt gaaactgaat gccgcgatcg
     6781 gcttcgcatt ccccacctga atggtctttg tcgcacttcc tgtcagcccg ccgttgtcgg
     6841 tgacaatgag cgttatctcg aactcgccct tctgggtgta ttggtgctct gggctctcct
     6901 gttcgctttc cgcgccatcc ccgaactccc aatgccacgc cactatctcg ccgtcatctg
     6961 tcgactcgtc gacgaactta accttgtcgc gccgtgtggc gggggacggg gtgaatctga
     7021 agtcggcgtt cggggcgagg tttacgatgg tgaactcgtc cgtcacgaac tcgtggaacg
     7081 cgcctgccgg gtcggtggcc ctagctctta cgaggaaggt ctcgcctgcc tcgcctactt
     7141 cgatggtgtt ccactcgaag gagccggtgt tggcaagccc tgtcccgagc tcatgccacg
     7201 tctcgccatc gcgagtccat gccagatcga tcaggagctc gtcttcgtca tcatcatggt
     7261 ccgtcgccac ccatgtgatc tcgtatttcc cgtgcagctc atctccgccc acaggcgccg
     7321 tgatcgcgac gctaggtgcg gcgttaacct tgatcgtcac tgggacggta gcaaacggat
     7381 agagcgggtt atcggccgct acacataagc gaagcgagta tgtgccgtac gctgcgtcag
     7441 gggatccgag cgtcagctgg acttcctggc ttccgccggg cacaatgtac cccgacgacg
     7501 ggttaaccct gaggatcgac tcgatcggcg cgaagattat cgctaagcca tcctcgatgt
     7561 agggggcgtt gtagaccacc tgcaggccat ctgtgcccgt ggcgttctca atacccaccg
     7621 tggcgcctgt cacgtcagcg gtcatctcga ggtattggaa tatgatcgtg ccgtcaggat
     7681 acagtatcgc ctggaatgta tagtccccag agtcagtccg ccccggcacc gcctggtatt
     7741 ccacgatgaa gctgcctgct tcctggtcgt gatagtaata gacgttgccg ccactacccg
     7801 gattgaggtc atcccagaag atcgcaatca ggtcgttggg gtcggatgaa tcaggtatgg
     7861 gagtattcgt ccattcgctc ctcgtcatcc cgaacgtcag gtatccgttg gagcagatgc
     7921 gaacctcagt cttcatctcc ccgtagaacg ggaaggggaa cggaagatcc acatcgacaa
     7981 aggactcgtc gtacagtttc accccggttc cgacgtcgct gatctcgaac caatcgaacg
     8041 cgggaccacc gtcatggttg ctgtccatcc agatgtaccc acacccggca ggttcgccat
     8101 tgggatcttt ggggaggtcc ggcacaatcg atatttcgaa attcatcggc gccacgcctg
     8161 tgttgctcac tgtcaagaca gcctgctctt cttcaccggc ttgaatgatc gcattgatct
     8221 cgcctggttc aaccccaagc acaggcagcc atttgactgt gactgcaagc tcggcaatcg
     8281 cctcgacttc gttttcatct attgcgtgga aggtcgcttt gtatgctccg acgtcagtgt
     8341 atacgtgctc tgttgcggct tcatgcacca ggtcgctgcc atcgccgaaa tcccaccagg
     8401 tatcaattat gtcggtagca cccggttcgg tcactgcatt gaatgccacc ttaagcggcg
     8461 gctcgcctat ggtcggcgtc gcagtggctt cggcaatggt aggccctgcc tcgatgtgga
     8521 cgatcaagtc cacgtagagt atggcgtttt cggggtcgtt agtccttatt ctcaagcaac
     8581 gcctcacatc atcgcccggc ttgcccagcg cttcagcgtt gatcgtgaca tccagttctt
     8641 cggtttcgcc catggcaact gtgccgtcta ttgaagacat ggctattccc ggcgcccatg
     8701 gcatttcggt gctcacgagg tacacgcggc tattatctat cgatacaacc cacaggttgc
     8761 catcgctgtt gagcgcgagt ccacctccat ggtactctcc gccgaatgga tgcgggaact
     8821 ggtgcacgac ttcgagcgcg tcgagatcga gcccgaatat catgtcggga gagccgttgt
     8881 tcgataccca caggatcccg tctgggtgcc atgcgagtcc ggctatgcct actgggaact
     8941 tataggcgcc gatcaccgag ccggggctct cgtggcttgg cccattgata tggtagatga
     9001 tgtcctcgtc ccatccgccg atgaagaagg tatcatcttc cgccctataa gcaaggcccc
     9061 gttgcgatga gtatgtccaa ggcccatccg tgatcttcat gaccactgcg cctgtttcag
     9121 ggtcaagtcc gtatatgccg ttgtctccgc ccacgttgac ctgccacatc aggtctcggt
     9181 tggcgtcata ggccatgtcg gccacccacg atcctgccca cggcgtacgg aatatcagcc
     9241 cggtgtgggc gccttccggc gtcacaacat gatccttctt ggcctgcgca tcggagatcc
     9301 agaggttgcc atcgaagcca acgccccatg gcatatcgat ctgggaaggc acaggccacg
     9361 acgtgattac gtctccaact gcgcccggct cgatggaggc tgcgattttc tcgaccaact
     9421 caggcgcgat cgaggcatag agtccttcag ctgttctcgc gttctcatcc atcgcgatgc
     9481 ccacacgctc gggcattgca atcgtcggag cgcctccgct ctttaccctc accttgaact
     9541 cgaggtcggc attgccctca acgttgccca aggcgacagt cttcgtgacg ctgccgcgag
     9601 gtgacaatgt gacttcgatc gtttcaggct cgcaagtcgc acgaggcagc cacttcgctt
     9661 ccacctcgaa gctggcctta tcctccacgc ccagctggtc aactactgag aaggttgcaa
     9721 tgtattttcc cggttgagta tagctgtgcg tcacatcaag ctcgcttgag gatgttccat
     9781 ccccgaaatc ccagccatag cttgcgatcg ggacttcagg cgcgtcaaac tccgcgttaa
     9841 atgcgacttc aagcgggggt tcgccaaacg tgggctccgc agttgcccgc gtgataacgg
     9901 gaggcgccgc cacctccaaa gccaccggca cgatgatcag cgggttttct atatcgttcg
     9961 tcccgagcac tacattggct tcgtgcctac cgggaccgag ccgctcgctg ttgactgtga
    10021 tactaatcgg cgctgtgcca cccgccggca cgctgccttc ttcaggctcc catgtgagcc
    10081 agtcgccagc accgatcggc ccaaggccgg tctccaccag gtacatggtg ttattggcct
    10141 gagatgcaac ccaaaggttg ccatcaggac cgaactcgca tcccgcgcct gagtaatcgc
    10201 cgttgtaggg atgcgtgaac tgtgcgataa cggcatggct tcccggatgg atcaaataga
    10261 tcatgtcagg cgatgagttt gcggtcgcca cgagtatgtc ggccacgggg tggtatgcaa
    10321 ggcctgcgat gcccaccggc agtgaccact gctccactat ctgaccgggc gtgttccacg
    10381 attcgccttt gatcttgtag atgatgtcct cgtcccaacc gccgatgtaa aaggtgtcat
    10441 cattgcggtt gtaggccagg ccgcgctggg aaatgcttgt ccaaccgggt ccagatattg
    10501 atcccacgac tgcgccatta gcggggtcaa gcttgtagat ggcgttgtcg ccgcctacat
    10561 tgacctgcca gatgtgctgg ccgtcgaatg ccaagtcgcc ggcccaggat ccgccgaaat
    10621 tggcagccca gctcgttccc gtgtatgccc cttcgggtgt cacgatgtag tcggctgtgg
    10681 gtgtggaatc ggggtcggat attaccaggt tctgtgtgtc aaacaagacc cccacacccc
    10741 aacctgagga gatagggctg ggacaccgcc atcttgttat cactgagccc acggtgtcgg
    10801 gaagccattc tgatctttca ggatttggat gcgctccgta cactcccagt gccgtccgag
    10861 cctgagcgtc tttgatatct tccggaagca ccagcctctc cggcatctgt gccaggctcg
    10921 ggaactcgcc gtttccgaaa tggagagcac cgggccccgc attggagaga gtgactgtcc
    10981 ctgtcgccgt ctgtccgacg ccgattgtga aattcaactg ccccggatgc cagctagcag
    11041 aggcggcctc cgcaacagtc accttcatcg tgacagtagt gatgtatcca tcgttgtcca
    11101 cagctgtgaa cgtagccgta tatactcccg cagctgtgta gacgtggctg gcaacgaatt
    11161 cgtggacgac cggggagcca tccccgaacg accaatgttt atcgactatc gaaccgtcgg
    11221 gatcatgtgc cgctccaacg aactggaacg atgttcccgg aggtccctgc tgaggattga
    11281 cagcgcatgc cctgatctcc ggcggactgt tcgggattat ctggatgttg acgggcacta
    11341 ttgtcctcgg ttttcggaca tcgttggtat ggagcacgac cgcgccatcc cgcgtacccg
    11401 atctgaccat cccgagatca aatgtgacat cgaggtcgat gctctctccg ggcgctacct
    11461 cgccctgggc gggcgctatc gacatccagc tgacaggttt gatctcgatt gccaggccgt
    11521 tgcgcatata gccttctcta ttattgagca cctcgaggcc atctgtgccg gtggcattct
    11581 cgatgccgat ggtgcctttc cccttatatg aactgataaa ccgcatatcg aggtattggt
    11641 acacaatctt gccatcggga taaaggatgg cctggaacgt gtattcgccg ctctcaggat
    11701 aacgcggcac tttattccac tcgactatga agcagttgtt gactgtatcg tgatagtaga
    11761 agacgccgcc atcggtcgga gcctggggcg gattgaggtc gtcccagtaa acggccatca
    11821 tgtcgtttgg ttcatccgag gtagggatgg gtttgtttgc ccatgcattt cccttcgtgc
    11881 caaacgtgag ataaccgtct gaacagatcc tgatggaggt cttcgtgtct ccgtagaacg
    11941 ggaagtcgaa cggaagatcg acaaccctgg aatcatcgcc tgtcagggga agtttcgtgc
    12001 cgatttcgcc gatttcaacc caatcgaaaa cgggtccgcc tgatgcgtct gaatcgcgcc
    12061 atatgtatcc gaacccgtcc ggtccgccga cgccgagcgt actaagcgcc ccgtcctctg
    12121 tcgggactcc gcccgtaaac gcctctgcgc tgaaaatcaa cgcggcctcc cccatattcg
    12181 tcacggtaag ggtctcggtc ttctgccgat gggcccgcac agcctgatgg aatgtctgcg
    12241 ggtccacgct cgcctccggc agatctgcca caacaatagt gacgctttct tcggcgatga
    12301 ggccatcatc atcggtcacg gtgaacgtgg ccgtatactc cccgtccgcc tcgtaggtat
    12361 gaactggcgc gaaaacgccc gttacggggt gactgccatc gccaaaatcc cattcgatag
    12421 cggctatttc tccatcggca tcttccgcaa ccgcggaaaa ctcgaccgca agcggcgcac
    12481 tgcctatccg cggatcagcc gaaatcgatc ttatcacggg cgggatgatg gatttggcaa
    12541 acatgaatgt atcgacatcg acctgcgggt tcacaggatc gttgctgagg attttgatcg
    12601 ttgccttcca agtcccctgt ggcaagttgt gggctgcaaa ggctgctgtg agcgtgtcgg
    12661 attcgcctgg cgcgactgtg cccgcagtct tgtccaacac gacccatccc ggcacgaata
    12721 gcacgccgag attgtcatgg acataattct cgtcgtaagc cacctgaagc cctatagtgc
    12781 cagttgcgtt ctcgattccg attgtagcct cgtcgagacg cgtccccatc atcttgtaga
    12841 ggtatgtgat gtttcccccc ggagtcagcg tggcctggaa cgtgtaaggt ccccctctgc
    12901 catagcgctg aacgtcctga tactgcacga cgaatttctg gtcgtcgccg taatagaaga
    12961 ccttgccatc gcctttctgc ggttcaaggt catcccagaa gattgcgatc aggtcatttg
    13021 gctctctggt atccggtacg gaatgattgt tgtacgcggt gccactgtcg ccaaatgtca
    13081 agtatccgtt ggaggaaatc cgtatcgaat ccttgatatc cccgtagaag gggaactcga
    13141 acgggaggtt gactacgacg ctgtcctctc cctcgagggt taccccggtt ccgacctcgg
    13201 aaatctcgaa ccattcgggc atgacgaccc ctgcctcatc gctgtctgtc cagaagtagc
    13261 catgctcatc agggcctccg gatcccagct ttcctatggg gatggctgtt ccggacgtgt
    13321 ctaccatgcc tataagctgg agttcggccg tgtaatcgag gctccccagc ccatcattac
    13381 tgatggtgat cgcctcttca caggtctctc cccattcgag cctggtatgg atctcagtgg
    13441 gctccactcc gatcacagac gcaaagaaga cgtgaatctg cacggcagcg ctggcgtcta
    13501 tgccagcggt attcaccacg tggaatgagg cgtcataaaa cccctcttcc tcgtaagtat
    13561 gtactgcatc ccattcgtgc acggtctcgc tgccatcacc gaaatcccac cacatatcga
    13621 ggatttcgcc ttcgggcgtc tcggccatcg cgctgaaact gacctcaaga ggcggcggac
    13681 cccacgtggg agttgcctct atcgaagtta ttatcggcgc tctctggccc gcgatggcat
    13741 ttgccaagtt gagccgaccc cctgtgagca ccttcccctc atactgcggg aagacgtcga
    13801 cgctacctaa gatcatatcc ttgatagtga ccgtatcatc ccccatgcca gcggttctat
    13861 agaggggcac atgcggataa atgctccgca agagcgcagc ggcgccagat acgtgcggcg
    13921 tggccatcga agtgccgtag aagaaggcgt aagcctcgct gggctctgcc tcgggtggat
    13981 caggcggaac ggtggacaaa atgtatccgc cgggagcgaa gaggtcgaca gtcctctcgc
    14041 catagcacgt gctccaccag ccggcgtaat tgcatggcat ctcattttgc atcatggcgg
    14101 ctactgaaat gatgttcgcc gaatcatagc tcgatggata gtgcggatgg atatccgtgt
    14161 tgtcaccgga gttgcccgcc gcgcatacaa cgagcatgcc cgatgcttct atggcgtcct
    14221 tgagcacctg ttggtatggt cccccgcccc aactgcagct cgctatctgc gcgcccttgt
    14281 ctgcagcata ctgcagggcg aggatggcgt cggatgtata gccgcccccg gggccaatga
    14341 actttagggg cataatcctg acgttccagt tgatcccagt cacacccata ctattgtctg
    14401 actgggcacc aatcgttccg gcgcagtgag ttccgtggtt atcgttgaga tatccatatt
    14461 cgtcacgttc atcggggtca aacacactgt tgtcattatt ggcaaaatcc caaccccaga
    14521 aatcgtcaac atagccgttg ccatcatcgt ccactccggg ttccccgttc atttccgact
    14581 cgttgaccca aatgttggcg ataaggtcgg ggtgttggat gtagcagccg ctgtcaatga
    14641 cggccacaat gatctcatct gtgcctgtat ggacggccca agcttcgggg gcatcgatgt
    14701 ccgcattatt gacgggatcg cccgacatct cggggtcctt gtactggcag ttctcattat
    14761 ggaggcccca catcctctcg aaataggtat cgtttgggac aataggggca tctgtgtgag
    14821 cttggtcttt gtagcaaatg tagtctcgtt cggcataaag aatccccgga gcattgctca
    14881 gccttgatat tgccgattca gggctgaccc tctcgggaag ttccacgatt ccgaattttg
    14941 caccgcttgg aaatgtcgcg gtagctttga agtcagcaac tcgatacatt gagtatccta
    15001 gccgctctat cgaattggtg gcagcatttg tagtaatcgt atctgaaaaa cgcactatta
    15061 ccttgttggg atgataggta tcaggaccgg gctgccatcc accacaaagc cctgcctgag
    15121 accatacatc cgaggcgcct tcaacagcag cgcaaggtat gaccaataac aggatcagtg
    15181 cagcaataaa catcgacctg tttctccgac tatcagccat atactgcttc gtctcctttc
    15241 cactcaggtt tcttcctaga tgttttggaa catttacatc aatgcaacaa ctattgaata
    15301 atcatgaaca acacgtttgg tcgacttgtg cctgcttcgg acatcacctc cagtctcgtt
    15361 gaacccgggc gcagaagcta tatgaggagt cgaactggca ttcgagcagt tctataagaa
    15421 catttatgcg acgagtggtg ggatgggagt taccgtaatt cctcccatat tacaggatat
    15481 gcgcccatag atgtcactga acaatacgac cactatacca caatcacttc taaaattaca
    15541 aagattcgat gaagaatgac aatatagcgc aaacacagca tgacgtagct gtcagtgttt
    15601 gtcactgccg ttttattcac ccatctgctt gagaaaaaaa ggcgaggccc actgtgtgtt
    15661 ggctcaactt ccgcaaccgg agagataatc ttgcggattt ggctactgca tgagattgag
    15721 ctcccaccaa ggacgcatgt gggctattga aatgccaaaa tagtgactac catggcgtaa
    15781 ttgtcaatgg ccatttgaga atcccctttt gtccatttta agctgaccat taacacccac
    15841 ggggtagaac cgtttttaca gcacgtttcg gacttgatcc cgaatcatca tttgggaata
    15901 tcctcatcac ccattcccgc cttcggattt cttcattgag cctcttggtt gttctcagcc
    15961 ttttccggag gcattggggc agaaccatta cagccattgc atcgtcaaat ccttcttcga
    16021 tgctccctga gactctacgg ctacggcatt gaccagtccc ccatggtcat ctgacaccac
    16081 aaggtctacg tgcgaaaggc ccctcacctt caaagacttg aagaagttgc gccacgagtc
    16141 ttccttctca ctatctgcaa acatgaaccc aaggctacgc ccacggcgat cagtgcgctg
    16201 tgattccaaa ccctgcctcc cttacggatc ttaatcacta tggcatcaac cattagaaat
    16261 ggatatggcc ctttgtcgag agaacgctcg cgccattcgc gcactgcagg tcaccatggc
    16321 tgagactgtc gatttcgaga cttctgcacc acatagctcg cgggttatct tgcagacctt
    16381 acgagtagac actccgtcta caaccatctc gacaagcgtg gcaatcaagg caatgaacat
    16441 gttcgatggt aggtatgagg caattgctgt gaagatatga gagttcactg aaaactcctt
    16501 attgagcacc gaaaacatga atgaaagggg tatcaattcc ctggaatgtt ctggtaacag
    16561 cttcgggtca tcgagtagcg agcggttagc ccggctccaa taatgtttgt gggcaaacgc
    16621 tccaggcacc cgttgatatc cgggtcgcgt ctgcccacat ctacatcatt tatggaaggc
    16681 tttcaggggg acccacggga ggtcgccaac ctctacctct cttcaagcgc cctctcgata
    16741 acttggccta cgtaatcgat ctcgtcctct gtaaggttgt tgtagaatgg tatggctatg
    16801 ctagtgcgcc ccagctcttc agtaaccggg aagtcgcctt cttgatatcc aaacatttcc
    16861 ctgtagaacg gctgcaagtg tatcggtgta aagtatgggc ggcaaccgat atcggcttct
    16921 ccgagccttg ccatcacgtg gtctctcact ttggactgca tctttggggt ttcctcatcc
    16981 catccaactc ggataacgta tacaaaccag ctcattctgg tgacttcctg agctatatga
    17041 ggcaagcgaa cccctgttat cccgctcagc cgctccttat acatcttagc cacggcagcg
    17101 cgtttctcga tgatctcttc tatgcgctgc atctgcacaa cgccgatcgc cgcagacatc
    17161 tcatccattc tgtagttata tcctaagcgt tcatgtgcca gccacacgcc gctctctccc
    17221 ctgccttgat tgcgcatgct agaacagagg aatgcaatgc gatcatcatc tgtaacgacc
    17281 atgccccctt cgcccgtggt aatctgctta ttgggataga aagcgaagac agccgcatct
    17341 gcgaagtcgg ccccccccgc cggcttgccc ttgtaatggg atccaatagc ctcacatgag
    17401 tcctctatga atacgagatc gttccgtctg gcgatctcgc gtatgacgtc cacttttgcg
    17461 ggctggccaa aggcatccac tgccaggatg gcttttgttt ttggcgtaat cgccgcttcg
    17521 atgagatcag gatctatgtt ggcagtgtcg ggttctatat cgacgaaaac tggttttgct
    17581 ccctcataca aaatgcaatt gctggaggca ataaagctga atggggtagt gatgacctcg
    17641 tcgccctggc caatgtcgta cgcgcgcacc agaagatgaa ggccgcttgt tccactgttt
    17701 acagcaatgc catgttttcg cccacaatag cttgcaatag ccatctcaaa ctccatcatt
    17761 ctcggtccga gagccaatgt gtatgaatta agcacgtcca gcactgcatc gatttcagcc
    17821 tgcgtaatgt cagggcgggc aaggttcact ttcatgactg tccctccatg ttgtctttgt
    17881 agttcaatgt tatagattct caactcaatg gggaacacct gcttgcaccc tgcgattatc
    17941 acttcagcca tccgtatagc atttaccctt tctgggtcag gattccgggc ggtcttgaaa
    18001 agtcaaatga ggatccccag catgccgggc aacactatcg cctagcacag agcagaaaga
    18061 ggcccgcaac gcc
//
LOCUS       DAOSYL010000048        17927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2596_length_17927_cov_7.099541, whole genome shotgun sequence.
ACCESSION   DAOSYL010000048 DAOSYL010000000
VERSION     DAOSYL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2596_length_17927_cov_7.099541"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1345..2682)
                     /gene="ssnA"
                     /locus_tag="PLU15_08060"
     CDS             complement(1345..2682)
                     /gene="ssnA"
                     /locus_tag="PLU15_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOO30915.1"
                     /translation="MGADLILGNGVVVDCGYPARVIEGGGVAISGEIIADVGRTEELR
                     RKYPEAGFEDVGGRLIMPGMMCAHTHLCRVFARGMQPDGPPAKVLSKVLEGSWWRLDR
                     KLTEEDIWTSAMMSLIWCVRNGITTVFDHHHSPNAAFGSLDAIADAVARVGIRASLSY
                     EVSDFDGPDAADEGIRENVRFIERCKRMKSPLIRANFGFQGSLTLSNETIRKCVEAPG
                     AVEAGFHIHTAEGIEDLIRSIRRYGKPVVKRLDDLGVWNDRSLAVHCVHINSEEIEIL
                     RERDVSVVHCPESNMGNAVGDAPILEMISRGVRVGLGTDGCSCGMFESLKAADLLLKH
                     KEADPQVGWIEAPLMAFRNNSAILAKQWDVPLGTLKPGAYADLIIVDYWAPTPIDDNN
                     WRSHVTMGVTGGMVDSTMVNGRFLMRHRELVAIDEREIAAKSRKLARELWKKV"
     gene            complement(3553..5955)
                     /locus_tag="PLU15_08065"
     CDS             complement(3553..5955)
                     /locus_tag="PLU15_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30916.1"
                     /translation="MMIEKLLVAARKGGPTIEAEFAKGLSVVLASTPQIRLIFLGALE
                     SMGVVGIEARTKRVEKGGRISVTVIDDNHRRVMLVRDSLSQPSVRVVDFDTGMGTEIA
                     VPIAEAMGLSETDLACSDDGMAECGAWTVPVRLLMGDNGRRYERAISLLDGISSDSGA
                     ENAYFELRREVERVADLRIKRAELRARIAWLVERLSFLDSRISSIDAALRTKVECDEI
                     EEYVGFLKQEFESWRINLADVLVTEKAAEKARNRLLKYSGMEGAFDPEVAGEIRNLMS
                     EMSELEKTAISYESEQSGITSAINGLRMELALASKDLEELEQRGLSRRTLEHAQSSLK
                     EIEDRETRLASLSQRIAEVDKVLGRKRLADGLSVGGALLTVTSLTGLLLPENIFPWMS
                     LSEPLMGVLILLLSTGLMCTVYGVSTGIELDAKHGAKVRLEHDRANLSSQVAWHRRRL
                     DSLLHDMSINDYALAVEKMEWLKEKKECLLEEIKTLSGELARLRNEADLNRVRTWRAK
                     ERMGELMKVSGFSSPSMYLEAYEDYRRVEASYREAADRLEAALGGRSRESIERDIEIV
                     LEEIASAEAQRAAIADPRIEEKIWELSEEYAIKADERERLALELEQKSLELVDTEHTL
                     AGVDIWEVSSEAARAASELERARREGLAASLAQAAIEEMIEEKRAQAGARLAEVASDI
                     MAFFTGEPTAEISCAVEEDCVRCSFPRAADSESLIPVAVLAVRLGASEVLYGRGALPL
                     IIDYSSKGKGGRVGHKSAYQGLTLVWPALEKLGRTRQVILVLDGLEFAEQIPKSCEVL
                     TI"
     gene            complement(5903..7306)
                     /locus_tag="PLU15_08070"
     CDS             complement(5903..7306)
                     /locus_tag="PLU15_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30917.1"
                     /translation="MEPVSILHTSDLHLDHVLTEYRRDVRAVRRRELVDTFNFIVSEA
                     IRLNVRLVLIPGDLICADSVADSVVEEVRNGFARLGRSSIFVAVTPGEAEESGGLELL
                     REACSGENVHLFMAEEWSSVSPFPGVTVWGLRTTSRNSTMPVLSNLVVEGSGMHVGLM
                     HGTFAGTPGLDSSICPISPDALSQSGLDYLALGHYHNMVNCSVGKATCWYSGSPVHLN
                     FDTRGERHALLVSLREDGTRVNPMKVPSRQHRVITVDIAGKSGAEIRERLSDMANAEL
                     CLRVELEGRVDPALRPILAGLAREFEDRFFHLEVADRTELVVRGPKGEDRRRPGRGST
                     RKEVRLPRTDQPAGKNYHANCTGMDALERDLLEAEQTFTAKMVALLTQSEEANAAAPG
                     SFAGQKDSPESSFKARRPESEGSGDRSHVDNPYGVERWKEVVDCALALGLSALGEVFR
                     GDDREAVGCGEKRRPDD"
     gene            complement(7660..8778)
                     /locus_tag="PLU15_08075"
     CDS             complement(7660..8778)
                     /locus_tag="PLU15_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOO30918.1"
                     /translation="MWEAGAQEVLRDEMFWRIIRVINLLLASGKGMSVAELAAACEAT
                     PEVIRDDIQRLSEYMRAPLMSNLDQGCDACDDDDPLCPDQEVWSLASKDFAFPITSLS
                     VKEARTLLAILKGSEPSCGAATSACGGDAKRDGMGGAERAVSKLEASLIKAGSSDEVA
                     LAASRRVVKAGRTMYGEGQEESKLDALGLFVARREPIHISYVNASGQRSERDTCPVAL
                     VYDWRTCAWYLYGYNERHGGYRHYRVSRILGYGPSSVEIDAPSDEEVERHVKTCWGVE
                     CSCEQVEVVVRFLNDFNVIERMYADTADRPDAVYEVQTDGSVIYRDMMPGWNEFRTWV
                     ATFGESAEILAPPELRQSMAEAVDRVLDRYEKVGMLLG"
     gene            complement(8741..10075)
                     /locus_tag="PLU15_08080"
     CDS             complement(8741..10075)
                     /locus_tag="PLU15_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HOO30919.1"
                     /translation="MAGGRDTRLLSILEMVESSGGSCQVDTILEAFDISMRTLQRDLA
                     KLRMVGAKIEIRDALVVCEDDGGGYHARSSEAAMRQLAILTYLTGGPSTITSIVTYLN
                     DPIRDFSIDERTVRRDVDYLESRGLVEAPGQWSEPGGKTGPSCRGECVDRSGAYRLSS
                     AFMPRFSLTCDELVAVVLALESAPEAVADHATVVSIRDKLLASVAPQTGPAHYVTRRR
                     FTKGRTRRSDPAILRKVDTLELASLEGRVARIRYRGTGDGASKVRDVEPLGVVYYWFH
                     DAWYLVARCRRADELRHFRVDRIDGMMLTSDRYEYPEDFDLSDYTSEMWGVYAGEPTS
                     VKVLFFDEMNVVDRLHAEVLGRPSARLCQVDDGVWELVDTVMGLSEFRTWLRTFGSSA
                     VVIEPENLRNQLIASAERMKEIYSGPSVMAGAGEAKEDAIHVGGRRTGSPEG"
     gene            complement(10104..10742)
                     /locus_tag="PLU15_08085"
     CDS             complement(10104..10742)
                     /locus_tag="PLU15_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO30920.1"
                     /translation="MQNVALLPELQLIAAVQEENCGEAFAELLDRYSRAIYVWTKPFF
                     NEDVVSRDDLLQEARLGFLAAVRAYNPKLASFFTFAELCVNRRLCSVARSFRRSKNTP
                     PAPVLPLWGSARSWDSDDRNLIESVADTDAVDPVEEAIAHDSAARIMNVASSALSTLE
                     LEVFKGLAEGASYRELAVNMGVETKTVDNAAFRARRKLRELMFQEIERDVTA"
     gene            complement(10856..>12034)
                     /gene="scfB"
                     /locus_tag="PLU15_08090"
     CDS             complement(10856..>12034)
                     /gene="scfB"
                     /locus_tag="PLU15_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOO30921.1"
                     /translation="LAVLIDEGTLAWPDDEDPVPSALGPGVVKAMCLHVAHDCDMRCG
                     YCFAGTGGFGGRRSLMPPEIACAALDLLFDISGHVKHLDVDFFGGEPLLAMDTVVRAV
                     EHGRKLEAETGKVLKFTITTNATRLTPEIGKFLNDNQIAAVLSLDGRPQVHDAVRKGA
                     SGESTFDRCSANALSFVKTRPEGPDGEYYARGTYTRKNTDFVEDVLFLADMGFTSISF
                     EPVVSPPSSPYSIRESDLDEIYRSYDRLVREMDARSEEGRGFRFFHFDLDVAKGPCLA
                     KRLLGCGAGREYVAVTPDGELYPCHQFVGREGFVMGDVFHGIEKPEIGERFGAAHIYA
                     KEACRDCWARYLCSGGCHANAHQMNGTLMEPYAIGCMITRKRLESALAFAGLESLRCA
                     "
     assembly_gap    12035..12134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12135..12408)
                     /locus_tag="PLU15_08095"
     CDS             complement(<12135..12408)
                     /locus_tag="PLU15_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30922.1"
                     /translation="MPDLLPVCLQDIVYAWEPGLSEIADILDIERRRPSAAGIRRNWH
                     WFQAADARFLLDVQSGSLHAIDDAAWDVLEALVSSCDQAGTRECAAA"
     gene            complement(12339..12500)
                     /gene="scfA"
                     /locus_tag="PLU15_08100"
     CDS             complement(12339..12500)
                     /gene="scfA"
                     /locus_tag="PLU15_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOO30923.1"
                     /translation="MNTDSKKNTKHVKTIIASDLKSTVSSGGCRECQTSCQSACKTSC
                     TLGNQACRR"
     gene            complement(12531..13559)
                     /locus_tag="PLU15_08105"
     CDS             complement(12531..13559)
                     /locus_tag="PLU15_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOO30924.1"
                     /translation="MKDLVKRLTEAYGPSGSEDKVREIIKAEVEKFADEIRVDSLGNL
                     ICRVAPKSPADGSDPKRIMVAAHMDEIGVLVSYIDDKGFLRFSPVGGLSPYVLLGQKV
                     IFENGTVGVFGTEKLEDMKSLAFDKMFIDIGASSKDEAEKKVRVGDSAVILREFSDLG
                     DRVTSKTMDNRISCAVVIEAMKSVCPERHEAYFVFTVQEELGLRGAKAAAYGINPDVG
                     IAVDVTTWGDTPECAPFNVVLGKGVAIKAKDSSVIAHPKVKNLMIDVAEKKGILYQME
                     VLERGGTDAGAIHLTREGVPSGTISIPCRYVHTPCEMVDMRDVDAATRLLVGVVEELA
                     SSSGGGIL"
     gene            complement(13531..14583)
                     /locus_tag="PLU15_08110"
     CDS             complement(13531..14583)
                     /locus_tag="PLU15_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOO30925.1"
                     /translation="MLLAKLSEASGISGQEGEIRATIRNEIAPYVDEIKIDPIGNLVA
                     LKRGRTGKARVMLAAHMDEVGLMVVGFESNGLLHVDSVGGVDPRVLVSKMVCVGRNKL
                     PGVIGSKPIHLLEREDMDRATKMDALLVDIGAKNKEDAEKLVKLGDPVVFATKCEPLG
                     AGFMKGKAFDDRVGCCAIIEALKVQHDFDLYGAFTVQEEVGLRGAGVAAFQIEPDLAI
                     VLEGTTAHDVPETPEHSVSTVPGKGPAIVYMDRSWVGDKRIIDRLVETAEAASVPYQF
                     KRTVTGGTDAGRIGLTREGIPAAVVSMPCRFIHSPVSVINLSDFENYVKLAGLFLDSL
                     DERGLPIDEGSCKKTN"
     gene            complement(14574..15629)
                     /locus_tag="PLU15_08115"
     CDS             complement(14574..15629)
                     /locus_tag="PLU15_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOO30926.1"
                     /translation="MDTKQAILRLADARGVTGYESPAGDVVTALFADIVDEVRRDSLG
                     SVIMLKRGASPKPSAKIMVAAHVDEIGMIVSKIEDEGFLQVRMMGGVDRRILPSMEVT
                     VHGRRDISGVIGAIPPHLQDPKDKDKSWKWEELVVDCGMSAERLRELVRVGDVVSFDR
                     TGVELANDRISGKSMDDRAGVAVMYECAVELQKLKHDCDIYFVATVQEEVGYGGSITS
                     TYGITPDIGIAIDVGHGDMPGLPEHHTLKLGGGPAISYGPHVHPHIFSKLKEIAEERG
                     IAYQVEAGTSPRGTDAYAMQMTRSGVATGLISVPLRYMHTSVETLSVEDVKKSGQLLA
                     FFASAVDSRFREGLTCF"
     gene            complement(15829..16731)
                     /locus_tag="PLU15_08120"
     CDS             complement(15829..16731)
                     /locus_tag="PLU15_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOO30927.1"
                     /translation="MRIGLALGSGGLRGAAHIGVIDELERAGICPHMVAGTSSGSLIA
                     AMCGAGMSVAEMEEIALGVQARDIIDSPGTTLFYLAVPLALLFCGQKSRLPKGMLRGA
                     RLESFLKANFGDMKMTDMKMPCAVVSVDLHSGEKIVFTSEAVRSECFKGTVYVDGHVA
                     DAVRASCSIPGVFFWKEWDGRYLVDGGVREPVPARVLKELGCDYVIAVDLGYTGQADS
                     KVGDLPSIITQSLDILGEEVSDYVLFHYADVVVKPRLYNVALTDVARIPECIEAGRRA
                     AVEALPAIRRGIRRRRIRGLAQLV"
     gene            complement(17072..17156)
                     /locus_tag="PLU15_08125"
     tRNA            complement(17072..17156)
                     /locus_tag="PLU15_08125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17120..17122),aa:Leu,seq:gag)
     gene            complement(17460..>17927)
                     /locus_tag="PLU15_08130"
     CDS             complement(17460..>17927)
                     /locus_tag="PLU15_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOO30928.1"
                     /translation="HAVFLIAGDTVEVAKVYDAEMPEGDARIILVDTFKDEAEESLRV
                     AEALHDKLEGVRLDTPGERGGVTPDLVREIRARLDQAGFEHVKIFVSGGVTPERIPAL
                     VEAGADSFGIGSYISGASPIDMTMDIKEVEGKPVAKRGRIPGPIDNPKLVQVM"
BASE COUNT         4159 a         5642 c         3976 g         4050 t
ORIGIN      
        1 tgattaggat agacgtccag tccaacgatt tcgaggcctc cgctactcgt ccccgtccat
       61 tggcgtgcag aagctccgcc ttgtagctcc ggctacatcg ccccggcggg tggaccacct
      121 gattaagtgc cgacaggaat aacatgccgt agcgcctggc caagagatca taaccgacct
      181 ctgagccagg cacaagggaa tgcgtagtaa gtgatcatgt cttttacacg gttctcggca
      241 tctggtttcg gcacatgaac cccagcgggt ttcgaagacc agacatgtat cgcgcgaaag
      301 tcaacagaag tccatccggc ccctcagatg agtttcgcct aaaagccgcc atatccatat
      361 ctcccccaca gcgtcacaaa cctgctactt acacaggtta cgtggaaacc ccgctactgc
      421 gtagggtcgg ggagcaccct ataaacaggc tagcttgaag ccgtcctcgt ccttcctacg
      481 tggaattacc tctcattggc aggccaaaac ttcaaaaacc tgtcggagaa tactgtggct
      541 gcagaagata gaggtcgcat tcgtcctgga agagcacgtg atttccagcc gctggtgaac
      601 tcagggcctc cgtgattgcg tccattttgg ccatcgcctg cagatcatat actcgttttg
      661 acgcatgcgg tgggcgacta tgccgatatc caagagattt cagtaggtta tacatcatgg
      721 ctaaactgat ttcgacgcca tgttttcata gacactgaac atccgcgcta tcttggtaat
      781 gcaccagccc aggctcgaaa tcaaaaccat ctgggcactc agtgatactt cttctgcgca
      841 tggcccaaaa cattgaaacc tcgcacttgc caaattctct tgcaaatcgc atttacactg
      901 tgcttcctgt caccatatag gcgctttcag gcattacaga gcacagtcaa aaaccacgca
      961 ggacaggcgg tgatagatgt cggcatacaa cgggtgctaa aggcctttca gactatccga
     1021 tgaagtagct tggccgagca tctctggttc ttgtagcaac acccttaaca ttgattaaaa
     1081 aagacaggga aacatcccct gcctactcgt attccggcaa ggacagttgg atgggaaacc
     1141 cttgcaaagt cagttcccat caaccccctg cttttcttca tatcccttcg tcttcccaca
     1201 gcatcctaga ctgacgcgca ccacggcctg agatacccac ccgaggcaaa cccttcatgc
     1261 ctagtctagg cgcgaaggcc tcaccggggt atagcttcgt tctatcgaca cagaccgcgt
     1321 tctggcatga tcgttcaaag cagctcagac cttcttccaa agctccctcg caagcttcct
     1381 cgactttgca gcaatctccc tctcatcaat ggcaacaagt tcacggtggc gcatcaagaa
     1441 cctgccgtta accattgtac tgtcaaccat acctcctgta actcccattg ttacatggct
     1501 ccgccaattg ttatcgtcaa taggcgtagg tgcccagtag tccacgatta tcaaatcggc
     1561 gtaggcgcca ggcttcagcg tgccaagtgg aacatcccat tgcttcgcca gtattgccga
     1621 attgttacga aaggccatga gtggcgcctc gatccagcct acctggggat cggcttcctt
     1681 gtgctttagc agaagatcgg ccgctttgag gctttcgaac ataccgcaag aacaaccgtc
     1741 tgtccccaat cccactctga ctccgcgtga aatcatctcc agaataggag catctcccac
     1801 agcattaccc atattggact cagggcagtg aacgacgctc acatctcgtt cccttagtat
     1861 ttcgatctcc tcggaattga tatggacaca atggaccgcc aatgagcggt cattccatac
     1921 gccaaggtca tccaagcgct tcacaacagg tttgccatat ctccttatgc ttcgtatcag
     1981 gtcttcaata ccctccgcag tatgtatatg aaagcctgcc tcgactgctc ctggcgcctc
     2041 cacacacttt ctgatggtct cattggaaag tgtgagtgaa ccttgaaacc caaaattagc
     2101 ccgaataagc ggagatttca tcctcttgca ccgctctatg aacctgacat tctcccttat
     2161 gccctcatcc gccgcatccg ggccatcaaa gtcggatact tcatacgaaa gcgatgccct
     2221 aattccgacc cgtgctacag catcggctat ggcgtcgagg gaaccaaaag ccgcgttggg
     2281 gctatggtgg tgatcgaaga cggtagtaat tccattgcgc acacaccaga taagagacat
     2341 cattgcgctt gtccatatat cctcttccgt cagctttcta tcaagtcgcc accaagaccc
     2401 ttccaaaacc ttagaaagaa cctttgcagg gggcccatca ggctgcattc cccgagcaaa
     2461 cactctgcat agatgggtgt gcgcacacat catcccgggc attatcaacc tgcctcccac
     2521 atcctcaaaa ccagcctccg gatacttccg gcgcaactcc tccgttcgtc ccacatccgc
     2581 tatgatctcg ccggatatcg caacaccccc gccctctatc acccgtgcag ggtatccgca
     2641 gtccaccaca actccatttc ctaagatcaa gtccgccccc atcgtcctga cctcctattg
     2701 ccactggttc tatggcctct attgtctgtc cacgccccat acacctgcat aggcccatgc
     2761 aatttcttga cttctttcgt cctttgtggg atgggagttc cattccgacc agtcctacat
     2821 tgatattggc attaccagaa tgcaaggcgc atacagcaac caaaccgttt tttgccgata
     2881 ttgctaaagc gggctaggag cgttgtggat tgcgctagat gtccccttct tcgctgcaac
     2941 ttctgtgccg gcgtcatccc tcccaaggca aagcttgtta gacgtacaga agcccctgca
     3001 gcactgtgga tttctgggaa accgtacaaa aacgaagttt tcttctccaa gtaacttctg
     3061 atcaatctct gaactgccct gcattcctgt ccatgcttgc tatcactcat ttagcctatc
     3121 attccagcgt tgtgccgatt ttctggccga cccttgtcga aaaactcgcc atgtcctcaa
     3181 aaagcatgtc caaaaacagc catgatagga accgcctcgc tcatcgacag acttgttcat
     3241 catgggcaca tgcttgcatt cacaggcgaa agctgccgtc tacagcatgc cctcatccaa
     3301 ttgcaggccc cgaatttcgg tcctagacgt ggtggtcaac atatgaactt ttagattgcc
     3361 aaacagaaga cttgtctgga aagcactcac atactccttc taagatactc agacccaaaa
     3421 acactgagag cgatgctacc gcatcgcttc ccgttcgcat ttgcattccg aagtcacata
     3481 ttcgaacact ggctcgtcgt tttttcatct cctccgtccc gaccacaaca aacgcgaacc
     3541 cttggcaatc cctcatatcg tcaaaacttc acatgatttt ggtatctgct ctgcgaactc
     3601 caatccgtcc aatactaata ttacctgcct tgttcgtcca agtttctcca gagcgggcca
     3661 aacaagggtt aacccttggt atgccgactt gtgccctacc cgtccgcctt tacccttaga
     3721 agaatagtca attatcaaag ggagagcgcc tcttccgtat agaacctcgc tggcgccaag
     3781 ccgaacagcg agaacagcca caggaatcaa cgactccgag tcagctgccc tcgggaaaga
     3841 gcatctgacg cagtcttcct caaccgcaca agagatttct gccgtaggct cgcccgtaaa
     3901 aaatgccatg atatccgagg caacctccgc taatcttgcc ccagcttgcg cccgtttttc
     3961 ttcgatcatc tcctcaatgg ctgcctgtgc taagctagcc gcgagcccct cacgcctcgc
     4021 acgttccaat tccgaggcgg ctcgcgctgc ctcactcgat acctcccaaa tgtctacacc
     4081 tgccagcgta tgttctgtat ctacaagttc caaagacttt tgctccagtt cgagagccaa
     4141 ccgctcccgc tcgtctgcct ttatggcgta ttcctcgctc agctcccaga tcttttcctc
     4201 gatcctggga tcggcaatgg ctgcgcgctg agcctctgca gaagcaatct cttcaagcac
     4261 gatctcgatg tcacgctcta tactttcccg agagcgcccg cccagtgcag cctctagcct
     4321 gtcggcagcc tctctgtagg atgcttcaac ccttcgatag tcctcgtatg cctcaagata
     4381 catcgaagga gaagaaaaac cagacacctt catcaactcg cccatgcgct ccttagcccg
     4441 ccatgtcctg acccgattca agtccgcctc gttcctcaat cgagcaagtt cacctgacag
     4501 agttttgatc tcctccagca gacactcttt tttctcctta agccactcca tcttctccac
     4561 ggccagcgca tagtcgttta tggacatgtc gtgcagcaac gaatcgagtc tccttctgtg
     4621 ccatgccacc tggctcgaaa gattggcccg atcgtgctcc agtctcacct ttgcaccatg
     4681 tttggcatcc agctctatcc ccgtcgaaac cccatagacc gtgcacatta agccagtgga
     4741 aagcagcaaa atcaagactc ccatcagcgg ctcagacaag ctcatccacg gaaagatatt
     4801 ctctggaagg agaagcccag tgagagacgt gacggtcaag agtgcccctc ccacagaaag
     4861 gccatcggcg agtctcttgc gcccaaggac cttgtcaacc tctgcaatgc gttgcgacag
     4921 acttgcaaga cgagtctccc tgtcttcgat ctctttcaag gaagattgcg catgctccag
     4981 cgtcctgcgg gacaatcctc tttgttccag ctcctcgagg tctttgctcg ccagggcaag
     5041 ctccattctg agcccattta ttgcagacgt aatacccgac tgttctgatt cgtacgatat
     5101 cgctgtcttc tccagctcag acatttcaga catcagattc cttatctcgc ccgcaacctc
     5161 agggtcaaaa gccccttcca taccagaata cttgagcaac ctgtttctgg ccttttccgc
     5221 agctttctcc gtcaccaaca cgtcggcgag attgatccgc catgattcga actcctgttt
     5281 caagaacccc acatactcct cgatttcatc gcattccacc ttggtccgta gagccgcatc
     5341 tatggaggat atcctcgaat caagaaagga taacctctca acaagccaag cgattctggc
     5401 tctcagttcc gcccttttta tgcgcaggtc cgcaacgcgc tccacctcgc gcctgagttc
     5461 aaaataggca ttttccgctc ctgaatcgct gcttattcca tccagaaggg aaatagccct
     5521 ctcgtatcgc ctgccattat cgcccatgag caatctgaca ggcaccgtcc acgccccaca
     5581 ctcagccatc ccatcatcgc tgcaagccag atcggtttcg gaaaggccca tggcttcggc
     5641 aatggggaca gcaatctctg tgcccatgcc tgtatcgaaa tccacaaccc tcactgacgg
     5701 ttgcgataaa gaatcgcgca caagcatgac acgcctgtga ttgtcatcga tgactgtcac
     5761 agatattctg ccgcccttct cgacacgctt ggtccgcgcc tcaataccaa caacgcccat
     5821 agattccaga gcaccgagga agatcagacg aatctgagga gtactggcca aaaccactga
     5881 aaggccctta gcaaattccg cttcaatcgt cgggccgcct tttctcgccg caaccaacag
     5941 cttctcgatc atcaccacgg aatacctccc caagagcaga gagtcccaac gctagagcgc
     6001 aatccacgac ctctttccag cgctcaactc catatggatt gtcaacatgg ctccgatcgc
     6061 ccgaaccctc actttcggga cgccgcgcct taaagctaga ctcgggcgaa tccttttgcc
     6121 ccgcaaaaga cccgggagct gccgcattag cctcctcgga ctgcgtcagt aatgctacca
     6181 tcttggctgt gaaagtttgt tccgcttcga gtagatccct ctcaagcgcg tccatcccgg
     6241 tgcaattggc atgatagttc ttccctgcag gttgatctgt cctgggcaga cgcacttcct
     6301 tccgcgtact gccacggccc ggtctcctcc tatcttcgcc cttaggcccc ctcaccacca
     6361 attctgtgcg gtcggcaact tcgagatgga aaaagcgatc ctcaaattct ctcgccagcc
     6421 ctgccaagat gggtctgaga gccggatcca cgcgtccctc cagttcaacc cttaagcaca
     6481 gctcagcatt ggccatgtca ctcaggcgtt ctcgaatttc cgctcccgac tttcccgcaa
     6541 tgtcgacagt aatgactcga tgctgccgac taggaacctt catggggttt acgcgagtcc
     6601 catcttcacg cagactcacg agaagtgcgt gccgttcacc tctcgtatca aagttgaggt
     6661 gtaccggtga ccccgaatac cagcacgtcg cctttcccac cgagcaattg accatgttat
     6721 ggtaatggcc taaggccaag taatcgaggc cagactgtga aagcgcatcg ggcgaaatag
     6781 ggcatatcga agaatcaagc ccaggagtcc cagcgaaagt accatgcatc aaccctacat
     6841 gcatccccga gccctccaca accaggttgg aaagaacggg catagtggaa ttccgagaag
     6901 tagtcctcag tccccataca gtgacccccg gaaaaggcga aacggatgac cattcctctg
     6961 ccatgaaaag atgaacattc tccccagagc aagcctcccg aagcagctcc aaacctccgc
     7021 tttcttctgc ttctcccgga gtaaccgcga caaaaatcga actcctgcca agccttgcaa
     7081 acccattacg gacctcttca actaccgagt cggccactga atccgcacag atcaaatccc
     7141 ccggaatcag caccagtctc acattgagcc ggattgcttc actcacaatg aaattgaatg
     7201 tatccaccag ttcccgacgg cgtacagcgc gaacatctcg tctgtactcg gtgagcacgt
     7261 ggtcgaggtg aaggtcggac gtgtgcagaa tgctcacagg ttccaaaacc ctgccctccc
     7321 gcagttgtag aatgtgatga accgcaacac acaagatcgt aatgcgccgt aattcacgaa
     7381 atagcgacgt taatgttggt tatgcattgc cattaccata tgtataagct tagctttcaa
     7441 atgctaactc gcactgtcta tttcttcgtt acgccccctt tcccttcata actcttccgg
     7501 cataaagcga ggcgcgcccc gaagggcgcg cctgttaatg cttgcagtgc cgacaatatt
     7561 cttcattcag atgccgaatc acgctcggcg cgaccatgga aagcgccaac agcccgattc
     7621 tccccgcccg ctcccaagat catgtctgtc cacgtctctc taccccaaca gcatgccgac
     7681 tttttcatac ctatccaaca ccctatccac agcctccgcc atactctgcc tcaactccgg
     7741 aggcgcgaga atctcggctg attccccgaa cgtggccacc caggtgcgaa actcgttcca
     7801 gccgggcatc atgtctcgat agatcaccga cccatccgtc tgaacctcat acacagcatc
     7861 cggccgatca gccgtatcgg catacatcct ctcgatgaca ttgaagtcgt tgaggaagcg
     7921 aaccacaact tccacctgtt cgcagctgca ttccaccccc caacacgtct tcacgtgtct
     7981 ttcgacttcc tcatcggatg gcgcatctat ctcgacactt gacggaccat atccgaggat
     8041 tcgcgatacc ctgtagtgtc gatatcctcc gtgcctctca ttgtatccgt acagatacca
     8101 cgcgcacgtc ctccaatcgt agacgagggc gacaggacag gtgtcacgct cacttctctg
     8161 ccctgacgca ttcacatacg agatgtgtat gggttccctc ctcgccacga agaggcccaa
     8221 ggcatcaagc ttcgattcct cctgtccctc gccatacatg gtccgtcctg ccttgaccac
     8281 ccgcctgctc gcagcaagag cgacttcatc agatgaaccg gccttgataa ggctcgcctc
     8341 tagctttgac actgcccgct cagcgccacc cattccatcg cgtttcgcat caccgccaca
     8401 agcggacgta gcagccccgc atgaaggctc agacccctta agtatggcca agagagttct
     8461 tgcttccttc actgaaagag acgtaattgg aaaagcgaaa tccttagagg caaggctcca
     8521 tacctcctga tcgggacaga gaggatcgtc gtcatcacag gcatcgcacc cttgatccaa
     8581 gttgctcatc aatggggcac gcatgtattc tgacaggcgt tggatatcat cgcgaattac
     8641 ctcgggcgta gcctcgcatg cagctgcaag ttctgccacg ctcatgccct tgcccgaggc
     8701 caacagcaga ttaatcactc gtattatgcg ccagaacatc tcatccctca ggacttcctg
     8761 tgcgcctgcc tcccacatgg atagcgtcct ccttcgcctc cccagcccct gccatgaccg
     8821 aagggccaga atagatctcc ttcatccttt cagcactggc aataagctga tttcttaagt
     8881 tttcaggctc gataaccacc gcagaactcc cgaatgttcg cagccacgtt ctgaactcgg
     8941 aaagccccat caccgtgtcg acaagttccc ataccccgtc gtccacctgg catagtcgag
     9001 cggaaggcct tcccaacacc tctgcatgaa gcctatccac cacattcatc tcatcaaaga
     9061 acaaaacctt cactgaagta ggttcgccag cgtagactcc ccacatctcg gaggtgtaat
     9121 ccgataaatc aaaatcctct ggatactcgt atctgtcgga agtcagcatc atcccgtcaa
     9181 tccgatccac cctgaagtgc cttagctcat ccgcccttcg gcagcgagca acgagatacc
     9241 atgcatcatg aaaccagtag tagactaccc ccaacggctc tacgtctctg accttgctcg
     9301 ccccatcgcc cgtgccccga tacctgatcc tcgcgacccg gccctccagc gacgccaact
     9361 ccagggtatc aaccttccta agtatggccg gatccgacct tcgagtccgg cccttggtaa
     9421 accttcgtct agttacataa tgagcaggcc ccgtctgcgg cgccacactc gcaaggagtt
     9481 tgtcgcggat ggaaacaaca gtagcgtggt cagccaccgc ctcaggcgca gactccagcg
     9541 caagcaccac cgccaccagc tcatcgcagg tcagcgaaaa tcttggcatg aatgcactgc
     9601 tcagcctgta cgcgcctgat ctatcgacac attcgccgcg gcaggatggc ccggttttcc
     9661 caccgggctc gctccactgg ccgggagcct ccacaaggcc gcgagactcc aggtaatcca
     9721 cgtcgcgcct cacagtccgt tcgtcgatgc taaagtcgcg tatggggtca ttcagatacg
     9781 ttactatgct tgttatggtt gaggggccac ctgtaaggta tgtgaggatg gccagctgtc
     9841 gcatggcagc ttctgaggac ctagcgtggt atccgccgcc atcgtcttcg cagacgacta
     9901 gggcatcacg tatttcaatc tttgccccaa ccatcctcag tttcgccaaa tcgcgttgga
     9961 gagttcgcat ggatatgtcg aaagcttcga gaatagtgtc aacctggcat gaccccccag
    10021 acgactccac catctcgagt atcgataaca gcctggtatc tcttcctcca gccatatgca
    10081 atcacccaga agacaattcg acatcaggcc gtcacgtccc tctctatctc ctggaacata
    10141 agctcccgca acttcctcct tgcccggaat gctgcattgt cgactgtctt cgtttccaca
    10201 cccatgttta cggcaagttc tctgtaggac gccccttcag ccagcccctt gaagacttca
    10261 agctccaacg ttgagagcgc gctagacgcc acattcatga ttcttgctgc agaatcatgc
    10321 gcaattgctt cttcgactgg atcgacagca tcagtatcgg ctacagattc aatgaggttc
    10381 cggtcatctg aatcccacga tctagcagaa ccccatagcg gaagaaccgg cgccggaggg
    10441 gtgttcttcg aacgccggaa tgaccttgca acgctgcaca gtcttcgatt cacacacaac
    10501 tccgcaaatg tgaagaatga tgcaagctta ggattgtatg cgcgcaccgc cgctaggaat
    10561 ccaagccggg cttcctgtaa cagatcatcc ctgcttacca catcctcgtt gaagaaaggc
    10621 ttagtccata cgtagattgc cctggaatac ctgtcgagca actcggcaaa agcctcgccg
    10681 caattctcct cctgaacggc tgcaatcaac tgaagctcgg gcaacaaagc aacgttctgc
    10741 attgccatac ctcccagaat gtcgaagtcc tcggctagac accgaggaca tggtaacatt
    10801 ccggtccggc acaaacctgt cacatgagca aaaccttgcc tggcggcgcc atcactcagg
    10861 cgcagcgcaa cgattcgagg cctgcaaaag cgagagcgct ctccaggcgt ttgcgcgtta
    10921 tcatacatcc tatggcgtat ggctccatta aggttccgtt catttggtgc gcgttagcat
    10981 ggcacccgcc ggaacacaaa taccgcgccc agcaatcgcg gcacgcctcc ttagcatata
    11041 tatgcgcagc gccgaacctc tcgcctatct caggcttttc aatcccgtgg aacacatctc
    11101 ccatcacgaa gccctccctg cccacaaact gatggcatgg atagagctcc ccgtctgggg
    11161 tcactgccac gtattcccgc ccggccccgc acccaagcaa ccgtttggca aggcacggac
    11221 ccttagccac gtcaaggtcg aaatggaaaa aacgaaatcc ccgtccctcc tcgcttctcg
    11281 cgtccatctc gcgaacgagt ctatcgtatg acctatagat ctcgtcgaga tcggattcgc
    11341 gaatcgaata aggagatgat ggcggcgaaa cgacgggctc gaaagatata cttgtaaaac
    11401 ccatgtccgc gaggaaaagc acgtcttcca cgaaatccgt gttctttctc gtatacgtgc
    11461 cccgcgcgta gtactcgcca tcagggccct cagggcgagt cttaacgaaa ctgagcgcat
    11521 ttgcagaaca gcgatcaaat gtagattcgc cggaggcgcc cttgcggact gcatcatgga
    11581 cctggggcct tccatcgagg ctgagcaccg ccgcaatctg attgtcattg agaaacttcc
    11641 caatttcagg cgtgaggcga gttgcgttcg tcgtgatggt aaacttcaat actttccctg
    11701 tctccgcctc tagtttccgg ccatgctcta ccgctcgaac caccgtatcc atggccagaa
    11761 gcggctctcc gccgaaaaag tcgacatcga ggtgcttcac gtggccgcta atgtcgaaga
    11821 gaagatcgag cgcagcgcat gcgatctcag gcggcatcag gcttcgcctt cctccgaacc
    11881 ctcccgtacc agcaaagcag tatccgcacc tcatgtcgca atcatgcgcc acatggaggc
    11941 acatggcttt gaccaccccg gggccaagtg ccgacgggac tggatcctca tcgtcaggcc
    12001 atgcaagggt tccctcatca atcagcacag ctagnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnctgccg cagcgcactc ccgagttcca gcttgatcac aggaggatac
    12181 gagcgcctcc aaaacgtccc atgccgcatc atcaatggca tgcaagctgc cagactggac
    12241 gtcaagaagg aaacgagcgt ccgctgcctg gaaccagtgc caattgcgcc tgattccagc
    12301 tgccgacgga cgccttcgct caatatccag tatgtcggct atctccgaca agcctggttc
    12361 ccaagcgtac acgatgtctt gcaagcagac tggcaggagg tctggcactc cctgcaaccg
    12421 cctgacgaca ccgtagactt gagatcagat gctatgatcg tctttacatg cttcgtattc
    12481 ttcttgctgt cagtattcac gcttattccc ccgtttgacc gattacacgt ctagagtata
    12541 ccaccgccag aagaggatgc caactcttcc accacaccca ccagcagccg ggttgcagcg
    12601 tcaacgtccc tcatgtccac catctcgcag ggagtgtgaa catatctgca cggaatagag
    12661 atcgtccccg aaggcacgcc ttcacgcgtc aggtgaatag cgcccgcatc cgtccctccg
    12721 cgttccagga cttccatctg gtatagaatc cccttcttct cggcaacatc tatcatgagg
    12781 tttttgacct ttggatgagc gattacactt gaatccttcg ccttgatagc cacgcctttt
    12841 ccaagcacga cgttaaaagg cgcgcattca ggagtatcac cccaggttgt aacatccacc
    12901 gctatcccaa catcgggatt gatgccatat gccgctgcct tggcaccacg cagacccagc
    12961 tcttcctgga cagtgaaaac aaaataggcc tcgtgacgct ctgggcagac ggatttcata
    13021 gcctctatca ccactgcaca ggagatccgg ttgtccatag tcttcgaggt cactctgtcc
    13081 cccaaatccg agaattcccg tagtatgacg gctgagtccc ccacccggac tttcttctcg
    13141 gcttcatcct tgcttgatgc accgatatct atgaacatct tgtcgaacgc caaagacttc
    13201 atgtcctcca gcttctcggt gccgaacacg cccacagttc cgttttcgaa aatgaccttc
    13261 tgccctaaga gtacgtatgg gctgagcccg ccgactggag agaaccgcag aaatcccttg
    13321 tcatctatgt atgaaacgag cactccgatc tcatccatgt gagcggcgac catgatccgt
    13381 ttgggatcgc tgccgtctgc aggcgacttc ggggccacgc ggcagatgag gttgccgagg
    13441 gagtcgactc ggatttcgtc ggcgaacttt tcgacctcag ccttgataat ctccctaacc
    13501 ttgtcttcag accctgacgg gccgtatgct tcagttagtc tttttacaag atccttcatc
    13561 tatcggcaaa cccctttcgt caaggctgtc aaggaacagc ccggcaagct ttacgtagtt
    13621 ttcgaaatca gagaggttta tcaccgaaac cggcgagtgg ataaaccgac acggcatcga
    13681 gactactgca gcaggaattc cttcccgtgt caggccgatg cgtccggcat cggtgcctcc
    13741 cgtcacagta cgcttgaact gatacggaac acttgctgcc tcagctgtct cgaccagccg
    13801 gtcgattatg cgcttgtcgc ccacccatga gcggtccatg tatacgatgg ccggaccttt
    13861 acccgggacc gtggagacac tgtgctccgg ggtctcaggc acatcatggg ccgtggtgcc
    13921 ctccaacacg atagcaagat cgggttcgat ctggaaggcg gcaactcctg cccctctgag
    13981 gcctacctct tcctggactg taaaagcccc gtaaaggtcg aagtcgtgct ggactttgag
    14041 agcttctata atcgcacagc aaccgacgcg gtcatcaaat gccttgcctt tcatgaaccc
    14101 agcgcccaat ggctcacact tagtcgcaaa aaccacaggg tcacctaatt tcacgagttt
    14161 ctcggcatct tccttgtttt ttgcgccaat atccacgaga agagcgtcca tcttagtggc
    14221 gcgatccatg tcctcccgtt ccaatagatg gatcggcttg gatccgataa cgccaggcag
    14281 tttgttgcgc ccgacacaca ccatctttga gacgagaact cgcggatcca cgcctcccac
    14341 agagtccacg tgaagaaggc cattggattc aaaacccacc accatcaagc cgacttcgtc
    14401 catgtgcgca gcaagcatta ctctggcctt tcccgtcctg ccacgcttca aagcgacaag
    14461 gttgccaatc ggatcaatct tgatctcgtc aacataggga gcgatttcgt tccggatagt
    14521 ggcgcgaatc tcgccctcct gaccggaaat gcccgaagcc tctgaaagct tggctagaag
    14581 caagtcaatc cctccctgaa ccgggagtca actgcgctcg cgaagaatgc cagcaactgc
    14641 ccggattttt tcacatcttc aaccgagaga gtttcaacag atgtatgcat gtagcgaaga
    14701 ggcacggaaa tcagaccggt ggccacgcca gaacgtgtca tctgcatggc ataggcatcg
    14761 gtcccccgcg gagaagtgcc cgcttccacc tgatacgcta ttccgcgctc ctctgcaatc
    14821 tccttcaact tggagaagat gtggggatgc acatgcgggc catagcttat ggcaggccct
    14881 ccccccaact tcagcgtatg atgctccggc aaccctggca tgtctccatg accaacatct
    14941 atggcgatac ctatatcggg cgttatccca taggtgctcg tgatgctgcc cccgtaacca
    15001 acttcctcct gaactgtggc gacgaagtaa atgtcacaat cgtgcttgag cttttgcaat
    15061 tcaaccgcgc attcgtacat caccgcaaca ccggcccggt catccatgct cttaccggaa
    15121 atgcggtcgt ttgccaattc cacacctgtt cgatcgaagc tgacaacgtc gcccacgcgc
    15181 acaagctcgc gcagcctctc tgcggacatt ccgcagtcca caacgagctc ctcccatttc
    15241 cacgacttat ccttgtcttt aggatcctgg agatgcggcg ggatcgcacc tatgaccccg
    15301 gaaatatcgc gtcttccatg cacagtcact tccatggagg gcaagatgcg tcggtctacc
    15361 cctcccatca ttctgacttg caaaaaccct tcatcctcga tcttcgatac tatcatgcca
    15421 atttcgtcga catgagcggc gaccatgatc ttggcagagg gcttgggaga agctcctctc
    15481 ttgagcatga tcacgcttcc gagcgagtcc cgccgaactt cgtcgacaat gtcagcgaag
    15541 agggcagtca caacgtcccc agcgggagac tcgtacccag tcacgccgcg agcgtcggcg
    15601 aggcgcaaga tcgcttgctt cgtatccata acccgtcccc tttccgcacc cattaagtga
    15661 cgaccctgac ccacagctcc aggaccgcac accttttttg tattcgccat tgcatgcgcg
    15721 cttcctgcaa tcacataaag tggatcgtgc caaaagatgc atcgacaaga tggctctatg
    15781 caggattaat gtaaatgagc ccatcacttc cttccacaat gattccattc ataccaactg
    15841 cgcaagaccc cttatcctcc ttcgtcttat gccgcggcgg attgcaggca acgcttcaac
    15901 agcggctcgc cgcccggctt ctatgcattc gggtatccgg gccacatctg tcagagcaac
    15961 gttgtaaagg cgaggcttca caacaacatc tgcatagtgg aacaacacat aatctgacac
    16021 ctcctcaccg aggatgtcga gggactgtgt tattatggac gggaggtctc caaccttcga
    16081 atcggcctgg cctgtatatc caagatccac ggcgattacg taatcacatc ctagttcctt
    16141 gagaaccctt gccggaacag gctcgcgaac cccaccgtcg accagatacc tgccgtccca
    16201 ttccttccaa aaaaataccc ccggtatgct gcaggaggcc cgaacagcat cggctacgtg
    16261 cccatctaca tatactgttc ccttgaagca ctcgcttctt actgcttccg acgtgaacac
    16321 tatcttttcg ccactgtgca aatcgaccga tacaacagca cacggcattt tcatgtcggt
    16381 catcttcatg tcgccgaaat ttgccttcaa gaatgattcg agacgggcgc cccggagcat
    16441 ccccttagga agtctgctct tttggccgca gaacaaaagg gcaagaggca cagcaaggta
    16501 gaatagcgtg gtcccagggg aatcgattat gtctcttgcc tggacaccta aagctatctc
    16561 ctccatctct gccacgctca tgccagcccc gcacatggca gcgatcaggc tgccagaact
    16621 cgttcctgca accatgtggg gacatatccc tgccctctcc agttcatcga tgaccccgat
    16681 gtgcgccgcg cccctcagtc cgcccgaacc aagcgcaaga ccgattctca cttgtcacca
    16741 ctccttacag acacgcattg gcaattacgg tagctctaca tactacgata tgcaacacgc
    16801 cgtctacgag gaaatggacg ccggttccgg attgaacctc gggggtcgtc atccccaagg
    16861 ccaaaaggga aaggcagctt gcagatcccg ccatggtggc ttctgaaaga gatcgggctt
    16921 tcggaccaat gggtagtccc aatttccata gcgcttgccg cacaggccgt caagtcatcg
    16981 ttgtcattga ttggcgctct gcacacccta tggaaattga atagctcgtg tgcaaaacat
    17041 ggaggcgaaa agaatggagt acataaaaaa atggtgcccg ggacgagact tgaactcgta
    17101 cggtgtgaac cacacgcccc tcaaacgtgc gcgtctgcca gttccgccac ccgggcactc
    17161 aatctattct gctgttttca atgtccatca cagattatac gatcacaaag gcctcatgtc
    17221 aatagccgaa cgcatgtagc tgccaggccg cagcctccaa gcaagtcatg atcattctta
    17281 cacaccatct ctgagctaaa accacgtcgg gccgagcccc agctgcccga gaacactcga
    17341 ttgcctttca gaagccgatt taattgacta accctttaac acttctgtaa tatcccacta
    17401 ctgatccatc cagacgaggt aaagctcagg ctgcaactga gcacacgccc cttcccggac
    17461 tacatcacct gtacgagctt gggattgtcg attggtccgg gtattcttcc gcgcttggcc
    17521 acgggtttgc cttcgacttc ctttatgtcc atggtcatgt cgattggaga cgctcctgag
    17581 atgtagcttc ctattccaaa cgagtcggcg cccgcctcca ccaaagccgg aatgcgttca
    17641 ggagtgacac cgcctgagac aaatatcttc acgtgttcga aacctgcctg gtccaacctg
    17701 gccctaatct cgcggacaag atcgggagtc acaccgcctc gctctccggg agtgtcaagc
    17761 ctaacgccct caagcttgtc gtggagggct tcagcaactc tgagagattc ctcagcctcg
    17821 tccttgaatg tgtccacgag gattatcctc gcatcgcctt caggcatctc cgcgtcataa
    17881 accttagcca cttccacagt atcgccggca atgagaaaga cagcatg
//
LOCUS       DAOSYL010000049        17811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2621_length_17811_cov_8.475558, whole genome shotgun sequence.
ACCESSION   DAOSYL010000049 DAOSYL010000000
VERSION     DAOSYL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_17811_cov_8.475558"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..436
                     /locus_tag="PLU15_08135"
     CDS             221..436
                     /locus_tag="PLU15_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30929.1"
                     /translation="MPNVDSDKNYLVVSRPKEIDRMIYFGMSRFQWPYHEAYQAEISF
                     KDEIIPGRLHMYRRPRFLCPFIEHGVN"
     gene            complement(702..1724)
                     /locus_tag="PLU15_08140"
     CDS             complement(702..1724)
                     /locus_tag="PLU15_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOO30930.1"
                     /translation="MQIEITKSSDLLDSNGVLIQRGWAKDLLLRYNRERIRASKLKIK
                     EWDYYCILTEEYGAAFTIADLGYAGMGSVTFLDLTEPGEITQTVITGFPMGKLNMPTT
                     SHEGNVSFEHKNLNLEFIKDGRQRLINVFFANFADGKPLQGTIRMTQPKPMDTMVIAT
                     PFAGNPHAFYYNQKINCMPAEGILMLGSEKRAFDPARSFAVLDWGRGVWTYSNTWYWG
                     SASGLVDGTPFGFNIGYGFGDTSCASENMLFYNNKAHKLDQVQFHIPPDDYLAPWKFT
                     SNDRRFEMDFYPIIDRNADINALFFRSNQHQVFGRFTGRAVLDDGTVIELKDFLGFAE
                     EVKNRW"
     regulatory      2014..2200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2338..4566
                     /gene="cobU"
                     /locus_tag="PLU15_08145"
     CDS             2338..4566
                     /gene="cobU"
                     /locus_tag="PLU15_08145"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOO30931.1"
                     /translation="MGTSVSANAIGFAYGLAPVFSDVSFRLDPGRFLGVVGPNGAGKT
                     TLLKCLAGLLKPSAGEILLGGGCLSCLQREEIAKIVAVVPQDTRVGFDFSVEEFVMMG
                     RIPHLKRFSRETRKDREAVRWAIESTGMAPFAQRLVTSLSGGERQRAAIARALAQEPK
                     VLLLDEPTSHLDIRRQVEILDLVKIMSLERDITVVGVFHDLSLASMYCDEILMMTEGE
                     ARYSGTPEEVLTQENVGSLYGDKVVVTDHPVHSTPLVAPRSCALLNKDNEPGYVTGIE
                     NKPCEAGELGKSDRPDKSGRLTLITGGARSGKSSFAERMAAEAGTKVMYVATCEPMDE
                     EMRARVSRHRERRPKSWITVEEPLDVAATIANEASNYDAVLVDCLGLFVSNHLFAQDE
                     EADDQSKTEAVLAEVARFAEAARDAGSHVIVVTNEVGMGVVPAYPLGRLFRDVLGWAN
                     QIVAEEADEVYLCVAGEPVRIKPRGEAGVGERLRAVNRSESPRPRLERIRDLLIYNMP
                     DGAALVVSCDSAGAIGPKGRDVIATSGYVLGRYITRVALMELIACGAQPILVVDALCV
                     ERDPTGDEIIKGVRDEAALAGLDPEMAVTGSTEENIATSQTGIGIAAIGIAERSELRL
                     GRASVGDALVCIGVAKVGNEVTLDDPEIVDLGAVRALANIEWVHEIVPVGSRGIRAEA
                     EDLAETNGLRFRVKPGAHLDLDKSAGPATCVVAACDDAHWQELSAMIGKPVFMVGEVE
                     AP"
     gene            4563..5315
                     /gene="cobS"
                     /locus_tag="PLU15_08150"
     CDS             4563..5315
                     /gene="cobS"
                     /locus_tag="PLU15_08150"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOO30932.1"
                     /translation="MKRVIDRFAIAMSFLTVLPVYRGDAPWEDEIARSAVFFPVIGLV
                     IGLIVAAVDALALHAFSPLCAAVIGMMVQFWVTGGMHVDGLMDTADGMLSGRSADRIL
                     AIMHDGSVGPMGATVGVFIIILKVALAASMSGRTRTAALMCAPAMGRLSMVLSFCLFP
                     PATPERGMGRQLARKVGPGEFAITLSCTAALCFMLAGPRSLSAAACSLGVALFAGWRV
                     SSSIGGVTGDVYGALCEIAEVVSLAAFAFTAA"
     gene            5343..6008
                     /locus_tag="PLU15_08155"
     CDS             5343..6008
                     /locus_tag="PLU15_08155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO30933.1"
                     /translation="MVKRIYLVRHGETAATSGRRFEGSGPISGLGTPLSEKGAIQAEM
                     AADALAGEDVFGVYASPLLRTVQTATPISQATGVAIELEPDLAEGSFGAWEGMTFDEI
                     EHRYPLEVKRWFADPLGYAPPGGESVRQLAARVIPCIERIAGKSYGEEGTAVVVTHGG
                     PIRAVLSSCLCGNLEMFGSIPVDPGSISTLRCVGPGAAGIEVESMNVTSFSERGRAAR
                     FRG"
     regulatory      6029..6216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6335..7264
                     /locus_tag="PLU15_08160"
     CDS             6335..7264
                     /locus_tag="PLU15_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOO30934.1"
                     /translation="MFKKDWIRSLSAIVLAVLLTLVPGAIGAHAADMYPLSITDDLGR
                     KVEITSKPRRFVSLAPSWTEILFELGLGDQVVGVTQFCDYPAEAAKKTKVGGFSDPNI
                     ELIVSLQPDVVFGTTMHMKVAPEFEKRGIKFVCQNAQTVRGVMDNIMMAAKIAGVPDR
                     ADKVNGNIEKTIAQVTKAVAKIPVKERLSVLYLVWDEPVMSVGPNTLISDMITCAGGV
                     SITRDASSDYPSYSLEAIVAKNPDVILAPHVHSGGGLDPATLKSKAGWQTIDAVRKGN
                     VFLVDDNLVSRPGPRVGEGVMEFARALYPELFR"
     gene            7321..8361
                     /locus_tag="PLU15_08165"
     CDS             7321..8361
                     /locus_tag="PLU15_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO30935.1"
                     /translation="MRDRLGKGLMPILIVSLVVVSVLSLRIGAVELSGKEILAVIWDW
                     IVSTATGDCKGDSTPTEEIVLRIRMPRIVLAALVGASLALSGTVYQALFRNPMADPYV
                     IGASAGASLGATIAMMLPISVQTLHLGVVPLFAFIGSIASVATVFNLARVGERTPMVN
                     LILAGVAVGSILSSLVSLLMYLMPQDGLHGLMFWLMGGFSGRNWTHVLVMLPHLVLGL
                     TVIIYYWRELNAILLGEEEALSLGVDVGKVTRRLIAAASLLTAASVASSGIIGFVGLV
                     VPHLVRIVIGPDHKKLIPNACLLGAVFMLAADTVARSIIPPRELPVGIVTSLAGGPFF
                     IYLLRKYKVKEW"
     gene            8386..9087
                     /locus_tag="PLU15_08170"
                     /pseudo
     CDS             8386..9087
                     /locus_tag="PLU15_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
     gene            9212..10177
                     /gene="cbiB"
                     /locus_tag="PLU15_08175"
     CDS             9212..10177
                     /gene="cbiB"
                     /locus_tag="PLU15_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOO30936.1"
                     /translation="MSTGVMVVIAFLVDAAMGDPIRGTHPVVLMGRAIELLEKVLRPR
                     RAEPKTEKIMGAVLAATIVAGTYAVSRTLLNAVRGFCPHAGDILEIWLLSTALAARGL
                     CDSAMQVRAPLLDGNLPATRENLSMIVGRDTENLSEEEAVRATVETVAENTSDGVIAP
                     LLFGLIGGAPLALAYKAVNTLDSMVGYKDERYADFGWASAKLDDIANFIPARIAGLFL
                     VAAAAVTGHDCRGAWHVMLRDQRAHPSPNSGVGEAAVAGALGVQLGGVNTYGGQESVR
                     PHIGDAVKTLEPRHIEESVHLMYWASVMAMAAGAALRAVIDGIGV"
     gene            10185..11288
                     /gene="cobD"
                     /locus_tag="PLU15_08180"
     CDS             10185..11288
                     /gene="cobD"
                     /locus_tag="PLU15_08180"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HOO30937.1"
                     /translation="MVDYCIPLHGGNVHGAARARRVDVEKVLDFSANINPLGPSPLAT
                     EAIRSNLHMIRHYPDPDCTELREELARYLQADAVSVIVGNGGAEIIQMLAYVLRPQKA
                     LILSPTFSEYGRAVACAGGTVVRVPLRRDDGYSIDYDMVRANMDGVDMVWVCNPNNPT
                     GTLLPRKSILKLADIVARCGAALVVDEAFIDFVADSEEYSVRDEVQERRNLVVIGSLT
                     KFFAIPGLRVGYAIAPEDLADRLRRQAPSWNVNFLAQVAAVASLQDTEYMGSTRKFIA
                     RERSWLVRELEKLGAEVGVKVIPPSANFILLDMSESGLSSAKLCRRLSCSNILVRDCS
                     NFPFLSENHVRIAVRSRPENERLLEALREVFGE"
     gene            complement(11595..12416)
                     /locus_tag="PLU15_08185"
     CDS             complement(11595..12416)
                     /locus_tag="PLU15_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOO30938.1"
                     /translation="MNSSRLKAVAVCREINLSKIASHFGIERKLSWEDTLALNEQRLS
                     GILPQPADKSLFIFSFGSLVFVNFSESDIRDSIDYLAKVDRNIAGFAITKYTDDYQLT
                     VGGDKLSVTYDELIAPTLEPYYLELIAVILAKSAALERIEDELSKLFDDVEVVINELE
                     KGTMAISDERLAKLSGRILSYRYSSISYIMVLDKPDVVWRLQEAQELYLELEHVFELP
                     DRYGKVTHKTETLLDITGVFADLYHAKRGTRLEWLVIILIAFEIVLGLVEKFWPL"
     gene            13045..14184
                     /locus_tag="PLU15_08190"
     CDS             13045..14184
                     /locus_tag="PLU15_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO30939.1"
                     /translation="MKRLLKRLLVLVLAIAMLASATLSAAPPRIEAELNLITPVASFV
                     SVAALDAFKPWAKAKYGIDVKTNYTSKGTQLAVGMIREWGSNPQADVIWGGETVLYDL
                     LAADGFLVKHEVSQALLDRIPATMGQPKPMPLKDPKGFWTGTALEPYGIVYNEGLVTK
                     RLRTTPPQTWEDLVSNPRFKGQIAQCTPDNSSSNHATYEVILQKYGWEKGWEWLSKLA
                     AYTGQFVAKSGDVPAVVAKGEYALGFAVPSYMAFENYLHGGDIRFIAPPGAYVTPEPI
                     GVIKNCKNPNTAKAFIEFLLTDEGQKLFIDRGLFPIVPDLKVAGPAGSTAEKAVIFYG
                     GRRSYFDGVVENTYDNTLSARRESEVNRYFRENIFSKLDQLKAKY"
     gene            14264..15331
                     /locus_tag="PLU15_08195"
     CDS             14264..15331
                     /locus_tag="PLU15_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30940.1"
                     /translation="MNIRLIGVTKRFKTVEVLSKVDLHVRAGELFTLLGPSGCGKTTT
                     LRLIAGFYVPDEGKIMFGEREVQDIPTFKRNIGMVFQNYALWPHMTVYKNVAYGLQFK
                     KEPKSEWPRIVNEALVKVGLAGYEDRYPGQLSGGQQQRVALARALALNPDVLLLDEPL
                     SNLDAKIRASVRAEIRKIQQELGITTVYVTHDQEEALTLSDRIGIMREGQLVQIGTPH
                     DLYERPATRFVADFIGANNLVAGTVKATGDDIIVETELGLFKGYTHSELKPGDKCTLA
                     FRPENVEVSTTEPSGEDNWIQGKISFASYMGSTLRYEMDHPAGVLLKADVKNPLHHEE
                     LSWGSDVYFCFPKSAALVIAD"
     gene            15371..17437
                     /locus_tag="PLU15_08200"
     CDS             15371..17437
                     /locus_tag="PLU15_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO30941.1"
                     /translation="MSSGMSATGLRRWIRESKLWILPIYLFLIIFVIFPLVRLFVDSF
                     TTADIPLSALNEVVLLVDMLEEKVNAGEIEDALAASHDIALQLDRSVDVIGHMIDALG
                     RATFSQDELIAKWQKAQEDVAKEAAVMAELQDDPVAALAESRKATALIDEVVSLPRRA
                     FSVEFFLDVFRDELYMKALGNSLLLGLATVVTTSVIGFAVAYLLVRYDFPGRKVYNFL
                     TTVPIVMPPLVGVLGFIFILGRGGMVNELFYQLVDVLESSWPAMSNWLFDRLPFNFIY
                     GWHGLLLVETFHLFPLITLNVLDSLSKIDASLEEAAESVGSVGFKRLFDVTIPLTIPG
                     FVTGALLVFIGAFADFAAPMVVGLTNFIAPLAYMDIQQFTDRHLFKMGITVGAVMVVI
                     SVVLLLIAKKYISLKDYTTLSYRAVERKPLEGKKQAWVHVFLILLLIISYTPYLGVAL
                     ASFSRSWSMSPLPTAWTLAHYEKILLYAPGYIINTFQFCILAIIMCIVIGLPVAWIMA
                     RTDTWSSGALDILTTLVLALPGTALGIAYIRAFNTPILGSVPLANIWIIIPIVLAVRR
                     LPYTVRSSYGSLLVVHRSMEEAAQSVGAGGFKTFKDITLPLVWKGVLAGALFSFITSI
                     QESAATLLLAIPGWETMTVGIFTFYTGGTHGDAAALGFILIVVGVITLFIMDRLSSAT
                     AGGLFG"
     gene            17624..>17811
                     /locus_tag="PLU15_08205"
     CDS             17624..>17811
                     /locus_tag="PLU15_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30942.1"
                     /translation="MQQKETEFSVRLWTSYRLRIQTQAVKDGSNICCDLPGFESSVIM
                     RVGAQRGGGMQHSLRRATT"
BASE COUNT         3968 a         4416 c         5299 g         4128 t
ORIGIN      
        1 tgccgaacat taacatcaac ggcccggctg cactcgatga gctgctgcca tactctagga
       61 gcctgcccgg cgcctgccag acaccaagat agcaacaaaa tcatatccta gcccccgctt
      121 gatatctagg tgggggctat ttgacgctta cgattattga gatatgtata gagctgaagg
      181 aatgccttat ggagtcttca aggggatgga ggcttcccaa ttgccaaatg ttgattctga
      241 caagaactac ctggttgtat cgaggccaaa ggaaatcgac agaatgatct attttgggat
      301 gagccgcttt caatggccct atcatgaagc ataccaggca gagataagtt tcaaagacga
      361 gatcatacct ggaaggttgc atatgtacag aagacctcga tttctttgcc cgtttataga
      421 acacggagtg aactgaagag cagcacccgc acaatgctgg atttggccta cgaataggat
      481 gctcatggaa acgttgcgaa acagcctgca aatagtgttt gaagaccatt aggcggccaa
      541 cgtctcactc atggctatcg ccgtcggggc cggccaagtt cataatgact tgctcgggcc
      601 gtcttgtcta gttgggacac gcgaaacagg ggctgtgcgt ggccgtcaac cacatagtag
      661 gatagcgcgt atgggccgcc gcccacgggg gatgccctct gttaccatct gttcttgact
      721 tcctccgcaa acccaaggaa gtccttgagc tcgatgacgg tgccatcgtc cagcaccgct
      781 cttcccgtga acctgccaaa gacttgatgt tgattcgagc ggaagaacaa cgcgttgatg
      841 tctgcattcc gatcgatgat ggggtagaaa tccatttcga atcgccggtc attgctcgtg
      901 aatttccagg gcgcaaggta atcgtcggga ggaatgtgaa attggacttg atcgagtttg
      961 tgggccttgt tgttgtagaa taacatgttt tcggacgcgc atgatgtgtc tccgaatcca
     1021 tagcctatgt tgaaaccgaa cggcgtccca tctacaagcc ccgaggctga gccccagtac
     1081 cacgtattcg agtacgtcca tactcccctt ccccaatcca agacagcgaa ggatctcgcc
     1141 ggatcgaagg ccctcttttc gctgccgagc atgaggatcc cttcggcagg catgcaattt
     1201 atcttctgat tatagtaaaa agcatgaggg ttgccggcaa atggcgtggc tattaccatc
     1261 gtgtccatgg gctttggctg agtcatgcga atcgttccct gaagcggttt gccatcggcg
     1321 aagttggcga agaacacgtt gatcagcctt tggcgaccgt ctttaatgaa ctcaaggtta
     1381 agattcttgt gttcgaaaga tacattgccc tcatgcgacg ttgtgggcat gttgagcttg
     1441 cccatgggaa aaccggtgat cacagtctgc gtgatctcac ccggctcggt aaggtcgaga
     1501 aaggtcacag aacccatgcc tgcatagcct aaatccgcaa ttgtgaatgc cgccccatac
     1561 tcctccgtca ggatgcagta gtaatcccat tccttgatct tcagcttcga ggctcgtatt
     1621 cgctcgcggt tgtaacgtag caagagatcc ttggcccaac ccctttggat caagacccca
     1681 ttggagtcca acaagtcgga acttttcgta atctctatct gcacctcggc gccccctttc
     1741 tgtatcttgc cactgctttc gactgggatg gcccaattct tacagaggtg gttcaattct
     1801 cccccccatt ttcccatgaa accattcgac atcattgcct gcgttctcct gcctaagcga
     1861 ggtcaaggca cagaaattta tcatgcgttg taccggccca cgctcagctt gcaccatggg
     1921 tgggcttgcc ggatccctac tttagcggca tgtgcggatg gaaggacttc acttgcttcc
     1981 aaggctcaac tcgggtatag tttttgttga caactgaatc gacttcaacc aggtgcccat
     2041 agagggataa tagggaatca ggtgcaagtc ctgaacggtc ccgccactgt aaccggtgag
     2101 cgcgcgacat gagccactgt cacgagatga gatgggaagg ctgtcgccaa cacgctgtga
     2161 cccggaagtc aggagacctg cctgggttgg aagtaccgtg agccttcggc gcaagggcgg
     2221 gtactgcatg tgacaatcca gtcatgtaag tggttttagc ccgaccttta tcgaaaggtc
     2281 gggttttttg atacgacctg gtgggcactt gtggcatgat gaggggatgt gaggcagttg
     2341 ggcacgagtg tcagtgccaa tgccattgga tttgcatacg gtttagcccc ggtcttttcc
     2401 gacgtatcct tccgattgga tccggggcgc tttctgggag tagtcggtcc caacggcgca
     2461 ggcaagacta cactgctcaa atgtttggcc ggccttttga aaccatcggc cggcgaaatc
     2521 ctgctcgggg gcggctgcct ctcgtgctta cagagggaag agatagcgaa gatcgttgcg
     2581 gtggttcccc aggatacacg ggtcggattc gacttttcgg tggaggagtt cgtgatgatg
     2641 gggcggatcc ctcacctcaa gaggttctcg cgggaaacgc gcaaagatcg ggaggcagtg
     2701 cgctgggcga ttgaatcgac cggcatggcc ccttttgctc aaagactcgt cacatcactg
     2761 agcggtggag agaggcagcg tgccgccatc gctcgggcgc tcgctcagga gcctaaagtc
     2821 ctccttctag atgagcccac atcccacctg gacataaggc gacaagtgga gatcttggac
     2881 ctggtcaaga tcatgagcct cgaacgcgac attaccgtgg tcggggtatt tcatgacctg
     2941 agccttgcat ccatgtactg tgatgagatt ctgatgatga cagaaggcga agcccgctat
     3001 tctggcacgc ctgaggaggt tttgacgcag gaaaacgtgg ggagtctgta tggagataaa
     3061 gttgtggtga ctgatcaccc tgtgcactct actccgctcg tagcgccgcg ctcctgtgcc
     3121 ctgctgaaca aggacaacga gcctggctac gtaactggca tcgagaacaa gccttgcgag
     3181 gcgggcgaac tcggcaaatc agaccggccg gacaagtctg gacggctcac cctcattaca
     3241 gggggcgcaa ggagcgggaa gagctcattt gccgagcgca tggctgctga ggctgggact
     3301 aaggtcatgt atgtagcgac atgcgagccc atggacgaag agatgcgcgc tcgggtgagc
     3361 cggcatcgtg agcgaaggcc gaagtcgtgg attacggtgg aggagcctct tgatgttgct
     3421 gcgacaatcg cgaacgaggc gagtaactac gatgcggtgc tagtcgattg cctcggcctt
     3481 tttgtcagca accacctgtt tgcgcaggac gaagaggctg atgatcaatc caagacagag
     3541 gccgtgctgg ccgaggttgc aagatttgcc gaggcggcac gcgatgccgg ttcccacgtc
     3601 attgtcgtta ccaacgaagt tgggatgggc gttgtgcctg catatcccct cgggcgtctc
     3661 ttcagggatg tgctcggatg ggccaaccaa atcgtggctg aagaggcaga cgaggtctat
     3721 ctgtgcgtgg cgggggagcc cgtcaggata aaacctcggg gtgaagcagg agttggtgaa
     3781 cgattgcggg cagtcaaccg atccgagtct ccaaggcctc gccttgaaag aataagggac
     3841 ctcctcatat acaacatgcc cgatggggcg gcgcttgtcg tgtcgtgtga ctcggcaggt
     3901 gccatcggcc ccaaagggcg cgatgtgatt gcaacgtcag gctacgtact gggcaggtac
     3961 atcactcgcg ttgctctcat ggaacttatc gcatgcggcg cacaacctat cctggtggtg
     4021 gacgcgctct gcgtcgagcg tgaccccaca ggcgatgaga tcataaaggg cgtgcgggac
     4081 gaggcggccc ttgcggggct cgatcctgaa atggctgtga cggggagtac cgaagagaac
     4141 atcgctacct ctcagacagg catcggcatc gctgccattg ggattgcgga aaggtcagaa
     4201 ctgaggctgg gaagggcctc ggttggcgat gccctcgtct gcattggggt tgcgaaggta
     4261 ggcaacgaag tgactttgga tgacccggaa atcgtggatc ttggagcagt gagggctctg
     4321 gccaatatcg agtgggttca cgagatcgtg cctgtaggtt cccgtggaat tcgcgccgag
     4381 gcggaggact tggccgaaac gaacggcctc aggtttcggg tgaaaccagg cgcccatctc
     4441 gacttggaca agtcggccgg acctgcaacg tgtgtggttg cggcatgcga tgatgcacat
     4501 tggcaggagc tatcggccat gattggaaag ccggtattca tggtcggaga ggttgaggcg
     4561 ccatgaaaag agtaattgat aggttcgcga tagccatgtc gtttctgacc gtcttgcctg
     4621 tttacagagg ggatgcgccg tgggaagacg agattgcccg ctccgcagtc ttcttccccg
     4681 tgatcgggtt ggtgataggt ctcatcgtag ctgcggttga cgcacttgcc ctgcatgcat
     4741 tcagtccgct gtgtgctgcg gttatcggga tgatggtgca gttttgggtt acaggcggaa
     4801 tgcacgtgga cgggctcatg gacactgccg atgggatgct gagcgggcgc tcagctgaca
     4861 ggatcctcgc catcatgcac gacggcagcg tggggccgat gggggcgacc gttggagtgt
     4921 tcataatcat cctcaaagtt gcattggcgg cttcgatgag cggcagaact cgcacagctg
     4981 ccctcatgtg cgcacctgcc atgggacggt tgagcatggt tctgtccttt tgcttgttcc
     5041 ctcccgccac gcctgagcgt ggaatgggaa ggcagttggc ccggaaagtt ggcccgggag
     5101 agtttgcgat taccctgtca tgcactgccg cactctgctt tatgttagcc gggcccagat
     5161 cgctttcggc cgcagcctgc tccttaggtg tagcgctctt tgcgggctgg agagtatcga
     5221 gttccatcgg gggagtgacc ggggatgttt acggggccct atgcgaaatc gcagaagtcg
     5281 tgtcgcttgc ggccttcgct ttcacggccg catagctaga ggattggagt gctgagcgcg
     5341 ctatggtaaa acgtatctac cttgttcggc acggagagac tgctgcaacc tctgggcgaa
     5401 ggttcgaagg ttcgggccca ataagcgggc tagggactcc tctttcggag aagggcgcga
     5461 tccaggctga gatggcggct gatgccctgg cgggcgagga cgtttttggg gtgtatgcga
     5521 gccctctctt gagaacggta cagaccgcaa ccccaatatc ccaggccaca ggggttgcga
     5581 ttgagcttga accggatctt gcggaaggct ctttcggggc atgggaaggg atgaccttcg
     5641 atgagataga gcaccggtat cctttggaag taaagcggtg gtttgcagat ccattggggt
     5701 acgcgccccc cgggggcgag tccgttcggc agcttgctgc gagagtcatc ccttgcatag
     5761 agcggattgc cggcaagtcc tatggcgaag agggcacggc agtagttgtg acacatgggg
     5821 gccccatcag ggcagtcctt tcgtcttgtt tgtgtggaaa ccttgagatg ttcgggtcga
     5881 tcccagtgga ccccggatcc atcagcaccc tgcgttgtgt cgggccgggg gctgccggaa
     5941 tcgaggttga atcgatgaat gtgacatcgt tttcggagag aggtagagcg gcccgtttta
     6001 gaggataggc tcaagtgcgg gccaaaacag aatatcggtg caaacaggcg ccccgcaagc
     6061 cggggagaat agggaatcag gtgtgagacc tgagcggccc cgccactgta accgcgagcg
     6121 tgcagcatgg ccactgtcgg tgggagctat gggaaggcgc tggatttgac gctatgaccg
     6181 gaagccagga gacctgcctg tttgccgcgc ctgaaccttc ggcggaaagg gggcggtgca
     6241 tgggcaggcc gtttcgtctg tcatacacat gtgttgctgt ccggctttca agaggaggcc
     6301 ggacgtccat atttctacag aatgggggat tcacatgttc aaaaaagatt ggatcagatc
     6361 cctatcggca atcgtgctgg ctgtgttatt gacgttggtt cccggggcaa taggggcgca
     6421 cgccgccgac atgtacccat tgtccatcac cgatgacctg ggccgaaagg ttgagataac
     6481 gagcaagcct cgacggtttg tttctctcgc ccctagttgg actgagatcc tttttgagct
     6541 cggccttggt gaccaggtag tgggggtaac ccaattctgc gactacccag cagaagcggc
     6601 caagaagacc aaggtaggag ggttttcgga cccgaacatc gagctgatcg tctcgcttca
     6661 gcctgacgtg gtttttggaa ccacgatgca catgaaagtg gcacctgagt tcgaaaagcg
     6721 cggaatcaag ttcgtatgcc agaatgctca gacggtgcgc ggcgtaatgg acaatatcat
     6781 gatggcagct aagatagcgg gtgttcctga tcgagctgac aaggtgaacg gaaatatcga
     6841 aaagacaata gcccaggtga caaaagcggt ggccaagatc cctgtcaaag aacgtctcag
     6901 cgtgctgtat ctcgtatggg acgagccggt gatgagtgtc ggtcccaaca ctctcatctc
     6961 cgacatgatc acatgtgcgg gaggagtgag cattactcgc gacgcgtcct ccgactaccc
     7021 ttcatacagc cttgaagcca tagtggccaa gaatccagac gtgatactcg caccccacgt
     7081 gcatagtggc ggtggactgg atcctgcgac actgaaatcg aaggcgggat ggcagacgat
     7141 cgatgcagtg cgcaagggga atgtattcct ggtcgacgat aacctcgtgt cgaggccggg
     7201 cccgcgcgtt ggcgaaggcg ttatggagtt tgcgagggct ctctatcctg agctgtttcg
     7261 gtaaggagct ggcctggcag ctgggcagat ggcgagtata ggaaatgcag gtgagggcgg
     7321 gtgcgggata ggttgggcaa aggacttatg ccgattctga ttgtgtcttt ggtagtagtg
     7381 tctgtcttgt cattgagaat aggggcagtc gagctttccg ggaaggagat acttgctgtg
     7441 atatgggact ggattgtctc gacggcaact ggtgactgca aaggagactc tactcccacc
     7501 gaagagatcg ttcttaggat ccgaatgccc aggatcgtcc tggcggcgct tgtgggagca
     7561 tcccttgcgc tgtctggcac tgtgtatcag gcgctctttc ggaatccgat ggcagatcca
     7621 tacgtgatcg gggcgtcagc cggcgcatcc ttaggggcga caatagccat gatgctcccg
     7681 atcagcgtcc aaacgctcca tctgggagtt gttccgctct tcgccttcat cggctctata
     7741 gccagcgttg ctacggtgtt caaccttgca cgagtgggcg agaggactcc aatggtcaac
     7801 ttgattctcg cgggcgttgc tgttggatct attctctcgt ccctcgtgtc gcttcttatg
     7861 tatctcatgc cccaggacgg ccttcatggc ttgatgttct ggctcatggg gggcttttcg
     7921 gggcgaaact ggacacacgt tctcgtgatg cttccccatc tggttctcgg tctgacagtg
     7981 atcatctact actggagaga gctaaatgcg attcttttgg gtgaggagga ggcactgagc
     8041 ctcggagtcg acgtcggcaa agtcacaaga aggctcatag ccgctgcctc tctgctaacc
     8101 gctgcttcag tggcctccag cgggatcata gggttcgtgg gcttggtcgt tccacacttg
     8161 gtgagaatag tgatcggccc tgaccataag aaattgattc caaacgcatg tctgctcggc
     8221 gcagtattca tgttggctgc agacaccgtt gcacgatcta tcattcctcc gagggagctg
     8281 ccggtgggaa tcgtcacatc gcttgcgggg gggccgttct ttatctattt gttgaggaaa
     8341 tacaaggtga aggagtggtg atcagatttg cctgcggcca caatcatgat tcagggcact
     8401 acatcctctg cgggaaagag cgtgctttgc gccgggcttt gccgcctctt tcatcaggag
     8461 ggcttcagag ttgctccttt caaatcgcag aacatgtcca caaagtccgc gattacggct
     8521 gacggcggcg aaatcagcgt ttcccaggct tttcaggcgc ttgcttgtgg aatccaaccg
     8581 tcagcagaca tgaatccagt actgctcaaa cccctcggcg acatgaccag cgaggcaata
     8641 cttgctggca agagcgttgg cgtgatccac gcggcggaat accggtacag gcacttaggt
     8701 gtcgctctcg ggtgcattga gcggtcgtac cgtcgactcg cagctgaata cgacattgta
     8761 gtgatagagg gcgccgggag ccccgccgag gtcaacctga aggacaggga tatttgcaac
     8821 atgaggatcg cccggatggc cgacgctccg gttttactgg tggcgaatat cgatccaggc
     8881 ggggcgctgg ccgccatcgt gggcaccctc gagctgctcg atgcggatga gcgggacaga
     8941 gtaaaggggc ttgtgataaa caagttcagg ggagacttct cccttcttgc gccagggctc
     9001 gacttcctcg aaaagaagac aggaaaaccg gttttgggcg tgattcccta catcgataac
     9061 cttgccattg cggaagagga ctcgcttggc ggaaaaagag gaatctcttt gggggctatc
     9121 tcgacgctcg atgcggaatg cgacaggatt gcggcggctg tgcggaaagc catcgatatc
     9181 gatgagctgt tcgggattat ggggctgtag gatgagcaca ggtgtgatgg tcgtaatcgc
     9241 cttcctcgtt gatgcggcaa tgggggatcc aatacgtggt acgcacccgg tagttctcat
     9301 gggcagggcg atcgagttgc tcgaaaaggt tctccggcct agacgcgccg agcccaagac
     9361 ggagaagatc atgggagcgg ttcttgccgc tacaattgta gcaggaacct atgctgtctc
     9421 cagaacgctg ctcaatgcag tgcgcggctt ttgtccgcac gcaggcgata ttctggagat
     9481 ctggctgttg tcgaccgcct tggcggctcg aggcctctgt gatagcgcca tgcaggtgcg
     9541 cgcgccgctg ctagacggca accttccggc aaccagggag aacttgagca tgatagtggg
     9601 acgcgataca gagaacctca gtgaggaaga ggcggttcgc gcaaccgtgg agactgttgc
     9661 tgagaacacg tctgatgggg tgatagcgcc gctcctgttc ggattgatag gaggcgcgcc
     9721 gttggcattg gcatacaagg cagtcaacac cctcgactcg atggtagggt acaaggatga
     9781 acgttatgcc gatttcgggt gggcgtctgc aaaactggat gacatcgcaa acttcattcc
     9841 ggcccggatt gccggcctct tcctggtggc tgcagctgca gtgacgggac atgactgtag
     9901 gggcgcatgg catgtcatgt tgagagacca aagagcgcat ccaagcccaa actcgggggt
     9961 gggagaggca gcggttgcag gggccttagg cgtgcagcta ggaggggtca acacatatgg
    10021 aggacaggaa tctgtcaggc cgcatatcgg cgatgctgta aagacccttg agcctcgcca
    10081 tattgaagaa tctgtccact tgatgtactg ggcgtctgta atggcgatgg cggcaggggc
    10141 tgcattgcgc gcggtcatcg atggaatcgg ggtgtgagag tgtaatggtg gattattgta
    10201 tcccgcttca cggcgggaat gtgcatggtg cggcgcgcgc ccggcgcgtg gatgtggaaa
    10261 aggtgctgga tttcagcgca aacatcaatc ctctcgggcc ttcgccattg gcgaccgaag
    10321 ccataaggtc caatctgcac atgataaggc attatccgga tccagactgc actgagctgc
    10381 gagaggagct tgctcggtat ctacaggcag atgcggtgtc ggtgatagtg ggaaacggcg
    10441 gggcagagat tatccagatg ctcgcgtacg ttctgaggcc ccagaaggcg ttgatcctgt
    10501 cgcccacatt ctccgagtac ggcagagctg tggcatgtgc ggggggaacg gttgtgaggg
    10561 ttccgcttcg aagagacgat ggatattcca tagactacga tatggtccgc gcgaacatgg
    10621 acggcgtcga catggtgtgg gtgtgcaatc ctaacaatcc cacagggact cttttaccga
    10681 ggaaatccat cctgaaactg gcagacattg tggcccgatg cggggctgct ttggtggtgg
    10741 atgaggcttt catcgatttc gttgctgatt cggaagaata cagtgtgcgg gacgaagttc
    10801 aagaaagaag gaacctcgtg gttatcgggt ctttgacgaa gttcttcgca atccccggac
    10861 ttcgagtcgg ctatgcgatt gcccccgagg acttggcaga cagattgcgg cggcaggcgc
    10921 cgtcatggaa cgtgaacttt ctggcccagg ttgcagctgt ggcgtcattg caagatacgg
    10981 aatacatggg gtccaccagg aagtttattg cgcgcgagcg ttcctggctt gttcgagagc
    11041 ttgagaagct aggggcggaa gttggcgtca aggttatccc tccgtccgca aacttcatcc
    11101 ttcttgacat gagtgaatcg gggctgagct cggcaaagtt gtgtcgtagg ttatcgtgct
    11161 caaacattct ggtgcgcgat tgcagcaatt tcccattcct cagtgaaaac catgtgcgga
    11221 ttgcagtgcg tagtcggcct gagaatgaaa ggctccttga ggcgctcaga gaggtcttcg
    11281 gtgaatgagc agctccaaaa atgtttcagg tcaagcaaaa agccactgcc aggaaactag
    11341 cctccacttc atgggatgca atgaagattc cgcggataac gttacacacg ttttaagtcc
    11401 gaaagagtgc catatataga taggactgga cactaaatgg gtaactacca attgcgttag
    11461 cttgtatttt gatactacga ttcttcgcag tatggaccat gaacggcgtt tgcctgccac
    11521 tcaaaagctt tcacaacctg caaaaaggaa gaagcttctg cagcggcgtt tcgactggct
    11581 atggatgcgc ccgttcaaag tggccaaaac ttctcgacta ggcccaatac gatttcaaag
    11641 gcgatcagga tgatcacaag ccactcaagt cgtgtcccgc gcttggcgtg gtaaaggtcg
    11701 gcaaagactc cggtgatgtc gagaagggtc tctgtcttgt gagtcacctt cccatacctg
    11761 tccggaagct caaaaacatg ctcaagctcg aggtagagtt cttgcgcctc ttgtaatctc
    11821 cataccacat ccggcttgtc gaggaccatg atgtacgaga tggaactata gcggtagctg
    11881 agaattcggc cggaaagctt cgctaggcgt tcatcgctta tggccatggt gcccttttcc
    11941 agctcgttaa tgacgacttc gacatcatca aacagcttgc ttaattcgtc ttcgatccgt
    12001 tcgagggccg cggacttggc gagtatgacg gctatgagtt cgaggtagta cggctcgagt
    12061 gtcggagcta tcagctcgtc gtaggtgact gaaagcttgt cgccgccgac ggtcagctgg
    12121 tagtcatcgg tgtacttcgt gattgcaaag cccgcgatat tccggtcaac cttcgccaga
    12181 tagtctattg agtccctgat gtcgctctcg gagaagttga cgaataccaa agagccgaag
    12241 gagaagatga acaagctttt gtcggccggt tggggcagaa tgccagatag gcgctgttcg
    12301 ttgagggcga gcgtgtcctc ccatgatagt ttgcgttcta tgccgaagtg agatgcgatc
    12361 ttggaaaggt tgatctctcg acagaccgct acagccttaa gacgtgatga attcatcaaa
    12421 tgtcacgcta cagggccccc gtatggggaa gtagcgtttt ccctccttca taggccgatg
    12481 ccgaaatcct tgtgccatcc aacttgcgca ggtcaaaaat ggcctgtaac gcatcttcta
    12541 aaggcaaatt cctacgcctt cgagcgtgat catcaagggc tttggaatat tatatcactg
    12601 ctgttgtaag aattatatag ctccggggtg cgccaatggt ggtcgcctct tccatattca
    12661 tcagttcaaa atccatctac agagtcatcc cttgccgtga ggtgagcctt aatatgacct
    12721 taatggctaa tgacgcaata atgagtgaaa tcagagggga cgtgaaagaa atatctgcag
    12781 agtaggaagg agaatggcaa tgagactaga atattgcata ttagaagcca gatgaatgtg
    12841 gcatggagat atggagaggg ccactcatgg agcgctttcg cttcatgcgc gtgccctcat
    12901 tcctttcact gtcggacgag gctataaggc tggctgccaa ctgaataagt gctctgcctt
    12961 gatcaagaca ttgtggccgt gatgcattga tgagaacctg taactgctca gtgagcagat
    13021 taacaatgcg aggaggcaaa tctgatgaaa aggctactga aaaggcttct agtgctagtc
    13081 ctggccatag ctatgctggc atcagcgacg ctatcggccg ccccccctag gattgaggcg
    13141 gaactcaact tgattactcc cgtagctagc tttgtaagcg tggccgctct cgacgctttc
    13201 aaaccttggg cgaaggcgaa gtatggaatt gacgtgaaga ccaactacac gagtaaaggc
    13261 actcagttgg cagtaggcat gattcgagag tggggatcaa atccccaggc cgacgtgatt
    13321 tggggcggag agacggtgct ctatgacctc ttggccgctg atggcttcct ggtcaagcac
    13381 gaagtctcgc aggccctgct cgaccggata cctgcaacta tgggtcagcc aaagcccatg
    13441 ccattgaagg atcctaaggg attctggact ggtaccgcgt tggaacccta cggaatcgtg
    13501 tacaacgaag gtttggtcac gaaaagactt cggacgaccc cacctcaaac atgggaagac
    13561 ctcgtgagta atccacgttt caaaggccaa atagcgcagt gcacacctga caattccagc
    13621 tccaatcatg ccacctatga ggttatactt cagaagtatg gctgggaaaa gggttgggaa
    13681 tggctttcca agctggctgc ttacaccggc caattcgtcg cgaagtcagg cgacgtcccg
    13741 gctgtcgtag ccaaaggcga atatgcactg ggctttgcag ttccaagcta catggcattt
    13801 gagaactacc tccacggcgg ggatatccgg ttcatagcgc ctcccggcgc atacgtcacc
    13861 cctgaaccaa tcggtgtcat aaagaactgc aagaacccca acacagcaaa ggctttcatc
    13921 gaattcctgc ttacagacga aggacagaag ttgttcatcg atcggggcct gttcccgata
    13981 gtgcctgacc tcaaggtcgc cggtccggcc ggttccacag ctgagaaggc agtcatattc
    14041 tacggcgggc gcagaagcta tttcgatggc gttgtggaga acacttatga caacacgttg
    14101 tctgccaggc gagaaagcga agtgaacagg tacttcagag agaacatctt ttcaaaactg
    14161 gaccagctta aagcgaagta ctagtcgagt tgaagcaaat aacttcactg gtctgcgggc
    14221 ggggtctcgc ccgcagacca gccagtcgtt cgaggtgaag tgcatgaata tcaggctcat
    14281 cggagtcacg aaaaggttca agactgttga ggtcctgagc aaggtagatt tgcatgtgcg
    14341 cgccggagaa ctcttcactt tgctggggcc ttcaggttgt ggcaagacaa ctacgttgcg
    14401 cctcattgca gggttttacg tccccgatga gggcaagatc atgttcggtg agcgcgaggt
    14461 gcaagatatc cccacattca agcggaacat aggcatggtc tttcagaact acgccctttg
    14521 gcctcatatg actgtctaca agaatgtggc atatggcctt cagttcaaaa aagaacccaa
    14581 atcggagtgg cccagaatag taaatgaggc tttggtcaag gtgggattgg cgggatacga
    14641 ggatcgctat ccaggccaac tgtcgggagg acaacagcag cgtgtggcgc tcgccagggc
    14701 gctggcgctc aacccagacg tcttgctctt ggacgaaccc ttgtccaacc tggacgcaaa
    14761 gattcgggct tcggtccgcg ctgaaatccg aaagatccag caggaactgg gcataaccac
    14821 ggtctatgtg acacatgacc aagaggaagc cctgacgctt tccgacagga taggcatcat
    14881 gcgtgaaggt cagctggtcc agatcggaac cccgcatgac ctttacgaga gaccagctac
    14941 gcgttttgtg gcggatttta tcggcgccaa caacttggta gctggcacgg taaaggccac
    15001 aggcgacgac attatagtcg aaacggagct ggggcttttc aaagggtaca cccactccga
    15061 gttgaagcct ggtgacaagt gcaccttggc cttcaggcct gaaaacgtgg aggttagcac
    15121 cactgagcca tctggggaag acaactggat acagggaaag ataagcttcg ccagttacat
    15181 ggggagcact cttcgctacg aaatggatca tccggcaggc gtcttgctta aggccgacgt
    15241 caagaacccg ttgcatcatg aggagttgtc gtggggtagt gacgtgtatt tctgctttcc
    15301 gaaatctgct gcgcttgtca tagcggattg atacgctgat tccgaccatg gacgggcagg
    15361 tgattgtatt atgtcttcag gaatgagcgc aacgggtttg agaaggtgga tcagagagtc
    15421 caaactatgg atactgccca tctatctctt cttgataata tttgtcatat ttcccctggt
    15481 aaggctcttt gtcgattcgt ttacaacggc cgacataccg ctttcggcgc tgaacgaggt
    15541 ggtcctcctc gtcgatatgc ttgaggagaa agtgaacgca ggggaaatag aggacgctct
    15601 ggcagcgtca catgatatcg cgcttcagct tgacaggtct gtggacgtaa ttggacacat
    15661 gatcgacgcg cttggcaggg cgacgttctc tcaagacgag ctcatagcca aatggcagaa
    15721 agcccaggag gatgtggcga aggaagcggc ggtgatggcg gagctccaag acgatccagt
    15781 ggccgctctt gcagagtcgc gcaaggctac ggcactcata gatgaggttg taagtcttcc
    15841 acgacgggct ttctcggtag aattctttct cgatgttttc agagacgagt tatacatgaa
    15901 ggctctagga aattccttgt tgctaggcct tgccacagtt gtcacaactt cagtgatagg
    15961 atttgctgtg gcatacttgc tcgtaagata cgattttccc ggcaggaagg tctacaattt
    16021 ccttaccact gtacccatag ttatgcctcc tctagtggga gtgctggggt tcatcttcat
    16081 actgggcaga ggcggcatgg ttaacgagct gttttatcag ttggtggacg tcctggaaag
    16141 ctcatggcct gccatgtcta actggctttt cgaccgtttg ccgttcaact tcatctatgg
    16201 ctggcatggc ctcttgttgg ttgagacatt tcacttgttc ccgcttatta ccctgaatgt
    16261 cctggactcg ctcagcaaga tagacgcatc cttggaggaa gcggctgaaa gcgttggatc
    16321 tgtgggattc aagcgtctgt tcgacgtcac tatccccctg actataccgg gatttgtcac
    16381 cggggcattg ctcgttttca tcggggcatt tgcggacttc gcagctccaa tggtggtggg
    16441 cttgaccaac ttcatcgctc ccctggccta catggatatt cagcagttca ccgacagaca
    16501 cttgttcaag atgggcatta ccgtgggcgc ggtaatggtg gtgatctctg tagtgttatt
    16561 gctgatagcc aagaaataca tatccctcaa ggactacacc actctttcat acagggcggt
    16621 ggaaaggaaa ccactcgaag gcaagaagca ggcgtgggtc catgttttcc tgatcctgct
    16681 tcttattatc agctacaccc cgtacttggg tgttgcgctt gcgtctttct caaggagctg
    16741 gtcgatgtcg ccgcttccca ctgcttggac gcttgcacat tatgagaaga tcttgctgta
    16801 tgcaccaggg tatatcatca ataccttcca attctgcata ttggccataa tcatgtgcat
    16861 agtgatcgga ttgcccgtag catggatcat ggccagaaca gatacgtgga gcagtggagc
    16921 gttagatatt ctgaccacgt tggtgttggc tttgcccggc actgctttgg gcatagcata
    16981 catcagagct ttcaataccc caatactcgg gagcgtgcca ttggccaaca tatggatcat
    17041 aatcccaatt gtgttggctg tgagacggct cccatatacg gtccgatcct cctacggctc
    17101 cctcttggtc gtgcatcgtt cgatggagga ggctgcccaa tctgtgggcg cgggaggatt
    17161 caagacattc aaggacatca ctcttccgtt ggtttggaaa ggggtgctgg cgggggcgtt
    17221 gttctccttc ataacatcta tacaggagag cgcggcaaca ctgcttctgg ccataccggg
    17281 ttgggagacg atgaccgtcg gcatattcac cttctacacc ggagggaccc acggagatgc
    17341 ggctgctcta ggattcatcc ttattgttgt gggcgtgatc acgttgttca taatggacag
    17401 gttgagcagc gccacagcag gcgggttgtt cggatagcgc agccaatcag tggaatgact
    17461 ggacctcatg gagaggtgat tcaggaaaac gtctgggctg gcccggcagt atcgtgcctg
    17521 gcagtcggat gaataaatag ccgatcgtgg cggagcgcgt tgcgcaatgt tgtgatcggc
    17581 ttttcttttg cacgtcgtag ggcaacataa gaggctctgc gctatgcagc agaaagagac
    17641 agaattctcc gtgcggctat ggacttctta ccgcctgagg attcaaaccc aggctgtcaa
    17701 agatggctca aacatctgct gcgacttgcc cgggtttgaa tcctcagtca ttatgcgcgt
    17761 tggagcccag cgaggaggcg ggatgcagca ttctctgcgt cgggcaacta c
//
LOCUS       DAOSYL010000050        17002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_2798_length_17002_cov_7.455066, whole genome shotgun sequence.
ACCESSION   DAOSYL010000050 DAOSYL010000000
VERSION     DAOSYL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_17002_cov_7.455066"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..431)
                     /locus_tag="PLU15_08210"
     CDS             complement(108..431)
                     /locus_tag="PLU15_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003384432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOO30943.1"
                     /translation="MKLVIVIVRDDYSEVLQDELADAGHRYTKLASTGGFLRKGNTTL
                     LVGVDDADVDSVLEIVRATCKTRQELITTQPMPMSPGLAVPPIEVIAGGATVFVVEVE
                     HFERI"
     gene            complement(435..1760)
                     /locus_tag="PLU15_08215"
     CDS             complement(435..1760)
                     /locus_tag="PLU15_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO30944.1"
                     /translation="MSGEATQTQQEKREDLRWNFRMSTLDGMFSNVAMGMVTPFLAVY
                     AMELGGSNNAVGLITAVPALVNTLMYLPAASIVEQSDSRLKIASKTHLLAKSVYLLMA
                     FVPFLPWPDLRSGVLIGLLGLQTIPTVITMVAWTALMGETFPREERAHLFALRSMYTS
                     LVTLVSSVLAGVLLDSIEYPINYVTLFLISYATSLVSFYFLSKMKEYPATPVRSVSTS
                     LGDRIKRPFTNPDYGAKFRVFTISAVLVHLGINLAVPAYPIMYVKDLGLSKSAIGSLS
                     LAGGLTAVLAYPMWGVVSRKAGDAAVYALSLLAFAAFPVLYGMSGSLVYLLIMKAAMG
                     IFDAGFAFTLFNLTLEYVDPEDSANGIAVFNVLINATGIIGPPISAFVISRSGVMAAY
                     IVSSIVRTVGCVIFLKQFGVAETLGRLHRSLRLSLSRRRVTKQEKRGVL"
     gene            complement(1757..1948)
                     /locus_tag="PLU15_08220"
     CDS             complement(1757..1948)
                     /locus_tag="PLU15_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30945.1"
                     /translation="MKPQAINIDIRTGDILVEACTVAMAVSDASVGRAENAPRLPGER
                     AHDAEKTGKSLGTTKEVHR"
     gene            3006..3269
                     /locus_tag="PLU15_08225"
     CDS             3006..3269
                     /locus_tag="PLU15_08225"
                     /inference="COORDINATES: protein motif:HMM:NF022395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2619 domain-containing protein"
                     /protein_id="HOO30946.1"
                     /translation="MNDEITVERMAAIRATAGLIEVIGAVIILRLGTISKGLQVNAIL
                     ATIGPLFMLAVTGVGLAGLTGRISPAKVVLIGIGTGLIIAGSR"
     assembly_gap    3316..3415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3736..4635)
                     /locus_tag="PLU15_08230"
     CDS             complement(3736..4635)
                     /locus_tag="PLU15_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOO30947.1"
                     /translation="MEREVEPKFRIIYYPFMGTSVNTERLILLARLCYKPIGVDGLYD
                     MVWGTDSDPVDPGLQHMDDEAVGKFVRGLIRSGHLGALEHWNTSFAVEGYSRISSQQN
                     DRHRLMKTFKDPGVLQAEAAPEGGRAKEACEAWVNGPEQGSADVSQMQQSQRYVREDD
                     FKYVVPPSFHKDPEFLARYRKLQEDIAELQRYGLNEAKLPAEDVRFGLSNATETRFVI
                     TTNARQLRHMFNLRCCQRAQWEIRDLFDGLLREYKKIAPNIFWRAGASCEDFGYCPEG
                     SASCGRAPTIDQLLDSAGRKRED"
     gene            complement(4675..5154)
                     /gene="rlmH"
                     /locus_tag="PLU15_08235"
     CDS             complement(4675..5154)
                     /gene="rlmH"
                     /locus_tag="PLU15_08235"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOO30948.1"
                     /translation="MRIDVVAVGKMKERFLAEAVRDYERRISRYCRIEIREVAESPFA
                     ESDGEARKSQLRAGEAARLISRIRPGSYVIACDLAGKEMTSEQFAQFISDLGVAGVSD
                     LTFVIGGPLGYSPEVLAGANQRIAFSKMTFPHQLMRVILLEQIYRAFKIARNEPYHH"
     gene            complement(5154..6302)
                     /locus_tag="PLU15_08240"
     CDS             complement(5154..6302)
                     /locus_tag="PLU15_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO30949.1"
                     /translation="MGYYDRWGRPRGFGFPLFVAALVGAVIGGLVVGMLLIGRIPQQQ
                     AAPAPQLSTPSDDEILPPSERLSAEAYSIASAAKKVSPAVVKIATRQEELVFSFFFRP
                     VIQEREGLGSGVIIDASGHILTNYHVIKNVTEIVVVLADGRQFPGSVVGVDPYTDLAV
                     VKIEGEGLPVAELGNSDQIVIGEPVVAIGNPYGFDHTVTAGVVSALNRSISTDERDGV
                     VLENLIQTDAPINPGNSGGALVTTDGKVVGINTAIIQGAQGIGFAIPINVARNVANEI
                     IQYGKVKRPYLGVSEVVVVSRNIALRYGLAVDSGIYISSVVRGSPADRAGLGRGDIII
                     AINGAQVATADDVRRCVLKSSIGDQMTFTVVGTNRRQRDVVVTLGEMP"
     gene            complement(6359..7786)
                     /locus_tag="PLU15_08245"
     CDS             complement(6359..7786)
                     /locus_tag="PLU15_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOO30950.1"
                     /translation="MGSEIRFGTDGWRAVMCDAFTFANVRAVIQAVADYVISHDMRDR
                     GVLVGYDARFLSRGFAEVAAEVLAANSIPTLVTNRDTPTPVVAFTVADRGLAGAVMFT
                     ASHNPPEYNGVKWIPHYAGPADPEITNEIEGHIARRANAGSIPRTMPFEEALAAGVVR
                     SIDPSDRYLSRIRSLVALDIIAESGIKIVYDPLFSTGRGYVDRILAEAGCNVETINGC
                     RDPLFGGGSPDPQEESLGDLREKVLSSGADIGLATDGDADRFGAIDSDGTYLGANQII
                     PLLLLHLIRAGRGQGVVVRTIATTHLIDRIAHAHGIDVIETPVGFKYICQVMRERDVI
                     IVGEESGGLSIGGHVPEKDGILANLLLAEFRAVEGRPLKKSLRAVMDEYGASCTRRID
                     MRFSDNRKRDALIRRFVEKPPLEVAGRRVCDISTTDGVRFTLDGGDWFLVRASGTEPV
                     VRLYVESKSDEGILALLGEFNALLG"
     gene            complement(7908..8540)
                     /locus_tag="PLU15_08250"
     CDS             complement(7908..8540)
                     /locus_tag="PLU15_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO30951.1"
                     /translation="MKKCNTSAGEIRAAYGRIPNALVQAYTGDGKGKTTAALGLAMRA
                     LGHGYKVRMIQFMKGTSFTGELEAAKAFAPNFEIFQFGRDCKYSDKIRSGEAVCEGCG
                     DCFVRMGDPEPCDIEYARQAYELAGATLDEKAADLVILDEIANAVNFGLLSVEDAVSL
                     VERRPDGVELVLTGRDMPKTLIDMADLVTEMKMVKHPYMRGISARHGIEY"
     gene            complement(8576..9382)
                     /locus_tag="PLU15_08255"
     CDS             complement(8576..9382)
                     /locus_tag="PLU15_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO30952.1"
                     /translation="MRVEAVDVRYSYGPNLPDTLKGISLDVPSGSLYGIIGPNGSGKS
                     TLLRVIGGVARPCHGAVFYDGRNISELSPREAARQVAAVASGEPVIFPFTVEEIVMMG
                     RAPYLRPLQSESAEDITHVAQAMDRTGVTHLASRRIMELSTGERQRVLIARALAQQPK
                     VLLLDEPTAHLDVNYQVGIMELATSLAHDSGITTIAVLHDLNLAGRYCDRLAMIQDGQ
                     VVAEGCPDEVLTEANLLDVYGVRAVVARRPELDRPAVFVISGSSDGDGKG"
     gene            complement(9379..10443)
                     /locus_tag="PLU15_08260"
     CDS             complement(9379..10443)
                     /locus_tag="PLU15_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOO30953.1"
                     /translation="MTKPGRARLRILILAVAMVAVICWSASAGTAGVPAGVALRVLLS
                     KMPVIGRYVSLSEADVAPYDLESVEIIVTQIRLPRIVLGLLVGAGLSATGASFQGLFR
                     NPLADPYIIGASSGAALGACIGILAAGATGIGSLSAPSSYIPLFAFAGAVITVAFVYA
                     LATLDGCLPTDTFLLAGVVVGSFVWAAVSFLMVVAGEDLPKVVMWLMGSLSAKSWSHV
                     RITAPYVIGGTLALTALGRDLNLIAVGEEPARYMGLDVERFKKVIIVIGSLITAAVVS
                     TSGLIGFVGLVVPHIARMIIGPDHRSLIPVSAAGGAIFMVAADTLARVIIPPREIPVG
                     VITAMAGAPFFFYLLRRRKR"
     gene            complement(10558..11517)
                     /locus_tag="PLU15_08265"
     CDS             complement(10558..11517)
                     /locus_tag="PLU15_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOO30954.1"
                     /translation="MRSSFIKRTMASIVLALVVILAFSPVPLEASLHADGVDYAASTL
                     SSGFPVTIVDDTGYRLVIKKAPARIVSLAPSNTEILFSLGLGDRLVGVTTACDYPQAA
                     AKIDKIGDYTINVEAVVAKRPDLVVAQSDLQMPVVEKLRELGITVLAVAPKTMDEVFR
                     SMEMIGAAAGEGVRARKVVSSLETRVSAVEKAVAGLPESKRPVAFVEIWNDPLMTAGA
                     GTFIDDLITRAGGRNLAGDVDGWPQISPELVVASKPDVIILTCFNKAEVMARPAWQKI
                     PAVVRGAVYELVPDLLVRPGPRLVDGLEALAAIFHPSLFGLRK"
     regulatory      complement(11703..11919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(11974..13881)
                     /gene="selB"
                     /locus_tag="PLU15_08270"
     CDS             complement(11974..13881)
                     /gene="selB"
                     /locus_tag="PLU15_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOO30955.1"
                     /translation="MRYAIIGTAGHVDHGKTTLIKALTGVDADRLRQEQERGMTIDLG
                     FAPIMLPSGRVAGVVDVPGHERFIKNMLAGATGVDVVLLVVAADEGVMPQTEEHIDIL
                     QLLGVREGVVAITKADLVDDDEWMELVVEDVKERLASTFLANAPIICVSAATRTGLDT
                     LLAAIDSAVGRAVLQEEWSFTRLPIDRVFAISGHGTVVTGTLTGGEVKPGDKLELLPA
                     GKSVRVRQVQVHDSAVDEAVRGQRVALNLAGIDRANIERGMVLAKPGMIEPARIFDAE
                     LTLLESADVIEHGERMHLHIGAAEAVCRVRTIGTDGACPGDRVFVQLELEEEIAATRG
                     DRFIVRTYSPMKTAGGGRVLAVSRGRQRRFKEETLHWFETLSGDDESATARLFASSII
                     AQKSAPASAFDVARSMCADPEHVRDIFEEGVALGWAVAFGESPGTRTYMPRDDWDKAT
                     DAIIKALSEYHRREPLRAGMAKEELRTRTMSSWDGKPFSMLIAAAARDGLIEERGTLV
                     ALPWHTVSLSPKQRDEAERLEAAYSSSLMSPPERDQALAGETSEAILDYLIDEGILVR
                     VGPELLFHRDALSRAVEVARSLGEGGKPFTAAQFRDACGNTRKYAVALLEYLDSNRIT
                     VRVGDERVLAG"
     gene            complement(13881..15371)
                     /gene="selA"
                     /locus_tag="PLU15_08275"
     CDS             complement(13881..15371)
                     /gene="selA"
                     /locus_tag="PLU15_08275"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOO30956.1"
                     /translation="MNELMRAIPSISDVLSDPAAVELMAAYPRWAVRNAARELIDSER
                     RRFSSVAGQGVCSIDVQRQDVLARVVANLPSATVLAAAPGPRYVINATGIVLHTNLGR
                     APLPEYAARAVAECAMGYSDLEYDLEGGGRGSRQDHIARAMAELTGAPAAYVVNNNAA
                     AVLLCLDTLARGRKVLVSRGELVEIGGSFRVPDIMERSGATLVEVGTTNRTRLSDYRR
                     AIDSEAGLLLKVHTSNFRVMGFTEEVQLAELVELGRECGLPVMYDLGSGLVSDLGRFG
                     LHAEEPTVIEAVASGADLVTFSGDKLFGGPQAGIILGREDLIRKISVNPLARALRIDK
                     LTLSALAAVAAAWENPDDAGESIPVLSMLTQNTDELRERAASLMGLIEAGLDEREIPR
                     NLVDLAIVDDPSEAGGGSMPTVSIPGASVSVTSRCIPADQLQTRLRRCEPPVVVRIAE
                     GSVRFHMRTLFERDLKILAEHVCDILASPATFGGDTDLDFSEKEQD"
     gene            complement(15411..16433)
                     /gene="selD"
                     /locus_tag="PLU15_08280"
     CDS             complement(15411..16433)
                     /gene="selD"
                     /locus_tag="PLU15_08280"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(16413..16415),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOO30957.1"
                     /translation="MSTKAGUAAKIGPGDLQMAVRGLVVRKDPLLIAGFDGSEDAGVY
                     KLTPDIAIIQTVDFFTPIVDDPYEFGQIAAANSLSDVYAMGGVPIVAMNVVCYPCSLG
                     MEVLRDILRGGADKVYESGAVLVGGHSVDDKEPKYGLSVTGTVHPDKVVLNKGARPGD
                     VLVLTKPLGTGVISTALKGEMVDPHVERYAYTVMSTLNASASAVMQEIGVHACTDITG
                     FGFLGHLAEMADASDVSFTVYADAVPVIDGALDYIDMGLAPAGLHRNWEHVGPVVEVG
                     GNVDRNIVDAMCDPQTSGGLLIALASDKADELVRRLKDAGALAAAIVGEACRRGPEGP
                     TITVVS"
     gene            complement(16621..16717)
                     /locus_tag="PLU15_08285"
     tRNA            complement(16621..16717)
                     /locus_tag="PLU15_08285"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16681..16683),aa:Sec,seq:tca)
BASE COUNT         3792 a         5251 c         4218 g         3641 t
ORIGIN      
        1 gtgacaggac ttctttcagt ccatcggctc aacaaacatg ccgggcgaac gaaaaggcca
       61 cggaaacatc agttccgcgg cctaccccat gggtgatcct aagacttcta gatgcgctcg
      121 aagtgctcga cttcaaccac aaaaactgtt gctccccctg ctataacctc gattggagga
      181 actgcaaggc ctggcgacat aggcatcggc tgcgttgtga tcaactcctg ccgtgtcttg
      241 catgttgcgc gtacgatctc cagcaccgag tctacgtcgg catcatcgac gcccacaagc
      301 aacgtagtgt tgcccttccg caggaaccct cctgtggaag caagtttagt ataccgatgg
      361 ccagcatcag caagttcgtc ctgcaaaact tcggagtaat catcccttac aattacgatc
      421 acaagcttca cctattagag cacccccctc ttctcttgtt tggtcacgcg cctccgcgat
      481 agcgatagcc ttaatgatct gtgtagcctc ccaagagtct ccgctacgcc aaactgtttc
      541 aggaagatca cgcatcccac tgttcgcact atcgacgaca caatgtaggc cgccatcacg
      601 ccacttcggg atatgacaaa tgccgaaatg ggaggcccta taatccccgt ggcgttgatg
      661 agcacgttga agactgcgat cccgttggcg ctgtcctcag ggtccacata ctccagcgtc
      721 aggttgaaga gcgtaaaagc gaagccggca tcgaatatcc ccattgctgc cttcatgatg
      781 aggagataaa ccaaagaccc agacatgcca taaagcactg gaaaggcggc gaatgcgagc
      841 agagacaaag cgtatactgc ggcgtccccg gctttcctcg atactactcc ccacataggg
      901 tacgccagaa cagctgtaag accacccgcc agtgaaagac tgccgatagc gctttttgaa
      961 agcccaagat ccttcacgta catgatgggg taggccggaa ccgcaaggtt gatcccaagg
     1021 tgcacgagaa cggcacttat cgtgaatact ctgaatttgg cgccatagtc aggattagta
     1081 aatggccgct taatcctgtc gccaagcgaa gttgacacgc tcctaacagg tgttgcagga
     1141 tactccttca tcttggacag gaagtaaaat gacaccaaag acgtagcgta agatatgaga
     1201 aaaagcgtca cgtagtttat cgggtattca atcgagtcca gaaggactcc cgccagtacc
     1261 gaggagacaa gtgtaaccaa agaagtgtac atgctcctca acgcgaaaag gtgagcgcgt
     1321 tcctcgcggg gaaaagtctc ccccatcaac gctgtccacg ccaccattgt tattaccgtc
     1381 ggaatagtct ggagccccaa cagaccaatg agcacgccgg aacgcaggtc cggccatgga
     1441 agaaaaggca caaaagccat caacaaatat actgattttg ctaacaagtg tgtcttgcta
     1501 gcgatcttca gcctactgtc gctctgctcc actattgaag ctgcggggag atacattagc
     1561 gtgtttacaa gtgcgggcac tgccgtgatc aaccccaccg cattgttcga acctccaagc
     1621 tccatagcat agacagctag aaatggggtg accatcccca tggctacatt ggagaacatt
     1681 ccatctaggg ttgacattcg aaaattccac cgcagatctt cccgcttctc ctgctgtgtt
     1741 tgggttgctt cgccgctcac ctgtgcacct ctttcgttgt accaagactt ttgcctgttt
     1801 tctcagcgtc gtgggcgcgt tcccctggaa gcctcggcgc attctccgcc cgacctaccg
     1861 aagcatctga cactgccatg gcaacagtgc aagcctcaac caaaatatcc ccggtcctga
     1921 tatctatatt aatcgcctgt ggtttcacct cacttggccc cctacacggg aaggaacgcc
     1981 aacagacacg acacttctat ccactttgca gatggccctg aaatcgtccg attccagtcc
     2041 ctgatctcca tcacaatcca tccttgacca gaggttttac tgaaaattca gtcccatagc
     2101 ttcttcagcg cccaccaggt tccacctgca ctggctgcgc agaggcactg atcaagcctg
     2161 ctgattatga cagatcgccc aaatccatct gctccccact agcataatgc cttcgacacc
     2221 gagattcaac acacctgcta actaaccatg tcttgatgag ttcctgcgcg atgccgaagt
     2281 ctgtgctacc tgcggttcaa acctgatctg gaccaagatg ctacacccca aagcagctga
     2341 agcccttacg ccaagtagca atcgagaagc aacagtcaca caaacaccac cggaagactg
     2401 agctgccatt taagacccgg agaagttgtg tgtagcgatg gccaagattt gctgcggaaa
     2461 gaagcaatct ttgtcgacga acctagctag cagccctcac tcgcaacaag caatcaaaac
     2521 acatgtcaat agagctactc agattacttg acctgccttc gccctatatc cccacttcca
     2581 ccgggcagct ttcgcatgcg atagccgttt gaaagcgctc aatggctttt tcaaagctgc
     2641 ttggacttct tctatcccat cgactcaaca aacatgccgc gcaaagcgga ggaagttcgg
     2701 acaggatgga aacgcataaa cctgtggccg acgacaacca acgtattcag tatgcaaagc
     2761 tataccgcga tagacgtcga acctgcctgt cgaaacacag ccggcaacga tagagatatc
     2821 gatcatgcct caatgatcgg caaatgcgga tccggtcccc aaagtcttga cgacatctgg
     2881 cctgcaggct acggctatac tcctatccct gaatcaatcg ccgaaacaga tagagcatta
     2941 tgccgccggg ttcttcatac tatctcagta atgcaatcaa aatccaaaca tgtgagggat
     3001 gcgagatgaa cgatgagata actgtggaac gcatggcagc gatcagagca actgccggac
     3061 taatagaggt gatcggagcc gtaatcatct tgaggcttgg caccatctcc aaagggcttc
     3121 aggtaaacgc gattcttgcc actatcggcc cactcttcat gctggccgtg accggagttg
     3181 gactggccgg gcttacgggc agaatctcgc cggcaaaggt tgtgctcatc ggaatcggga
     3241 ccggcctcat catagccggt tccaggtagt cccggcctgc cgtcaatccg atctgaacac
     3301 cattgccatg ccagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttgg
     3421 tcctcagagc tcttgcaaca cgccgcatgt gcctgctcgc tactgtgctc gccccatagc
     3481 gtattcccct gtacccatct taactgagtt ttgagagaac cctttgcgtt cgggcatttc
     3541 tcgctcatcc ccggatgatg aagcttggaa tatgaggagc gacgccacga taacgcctgt
     3601 cttttcatgt atccagcatg aaacggccca atgtgggtcg tttttgcaaa gtgacttggt
     3661 gtgtcagaac cccggccctc agacgctccg aggtgtcatt caagtcatcg aaatggccgt
     3721 aaagccgcat atgggtcagt cttctctctt gcgccctgcg ctgtccaaca gttggtcgat
     3781 ggtaggcgcc cgcccacagc tggccgatcc ttccggacaa tagccaaaat cctcacagga
     3841 agcgcctgcc cgccagaaaa tgttcggagc gatcttctta tactcccgca gcagcccgtc
     3901 gaagaggtcg cgtatctccc actgcgccct ctgacaacac ctgagattga acatatgccg
     3961 cagctgtctg gcattggtag taatcacaaa cctcgtttcc gtggcgttgg agagcccgaa
     4021 cctcacatcc tcagccggaa gtttcgcctc gttcaagccg tatctttgga gctccgcaat
     4081 atcctcctgc aacttcctgt atctagcgag gaactcggga tccttatgga aactcggagg
     4141 cacaacgtac ttaaaatcat cctcccgaac atacctctgg gattgctgca tctgagatac
     4201 gtcggccgag ccctgctcag gtccgttcac ccaggcttca catgcctctt tcgcccggcc
     4261 cccctcaggg gcggcctcgg cctgcagcac acctgggtcc ttgaacgtct tcatcagcct
     4321 gtgcctgtca ttttgctgac tcgatattct gctgtatcct tccacggcaa agctggtgtt
     4381 ccaatgttcc agtgccccga gatggcctga acgtatcagg cctcgcacga acttcccgac
     4441 ggcctcgtcg tccatatgct gaagcccagg gtcaacagga tcactgtccg tgccccacac
     4501 catgtcgtac agcccatcca ctcctatcgg cttgtagcac agcctggcca ggagaatcaa
     4561 acgttctgtg ttgacagatg tccccatgaa cggatagtag attattctga acttgggctc
     4621 aacctcccgc tccatactaa tcactcccta agcgcacagc ttatccacag ctgatcaatg
     4681 atgatatggt tcattccttg ctattttgaa agccctgtat atttgctcca gaagaatcac
     4741 ccgcatcaat tgatgcggga atgtcatctt cgagaaggct atcctctgat tcgctccggc
     4801 aaggacctcg ggggaatatc caagaggacc tccgatcacg aaagtgaggt cgcttacgcc
     4861 tgcgacgccg agatcggaga tgaactgagc gaactgctca gaagtcatct cctttccggc
     4921 gaggtcacaa gctattacat acgagcctgg ccggattcgt gatatcaacc tggcagcttc
     4981 gcctgcccgg agttgacttt ttctggcttc gccgtccgat tcggcaaacg gcgactccgc
     5041 cacctcgcgt atctcaattc tgcaatatcg ggatattcgc cgctcataat cacgcactgc
     5101 ctcggcaagg aatctctcct tcatcttgcc aacggcgact acatcaatgc gcatcacggc
     5161 atctcgccca aggttacaac gacgtctcgc tgccgcctgt tggtgcccac aacagtgaac
     5221 gtcatctggt cccctataga tgattttagc acgcaccttc ttacatcatc cgccgtcgcc
     5281 acctgtgcgc cgttgatagc gataatgatg tcccctcttc cgaggcccgc acggtcagcg
     5341 gggcttccac gaaccaccga cgaaatgtag atgccgctgt ccacggccag tccgtatctt
     5401 aacgcaatgt ttctgcttac aaccactacc tccgagactc cgagatacgg cctttttacc
     5461 tttccatact ggatgatctc gtttgccacg tttcgagcca cgttaatggg aatagcaaac
     5521 ccgatgccct gggctccctg tatgatggcg gtgttgatcc ccaccacctt accatctgtg
     5581 gtcaccaaag cccctcctga attgccaggg ttaataggag cgtcagtctg aatcaagttc
     5641 tccaaaacta ccccatctct ctcatccgtg cttatggaac ggttcaaggc gctcaccact
     5701 ccggcggtta ccgtatgatc gaacccatac gggtttccta tggccacgac aggttctccg
     5761 atcacgattt ggtcagagtt gcctagttct gcaacgggca atccctctcc ttcgatcttg
     5821 actactgcaa ggtcagtgta gggatctacc cccaccacac ttccgggaaa ctgacggcca
     5881 tctgccagca caaccactat ctctgtcacg tttttgatga catggtagtt ggtgaggata
     5941 tggcctgacg catcgattat cactccggaa cctaaccctt ccctctcctg tatgaccggc
     6001 ctgaagaaga aggaaaagac caattcttcc tggcgggtgg cgatcttcac cacagctggg
     6061 ctcactttct tggcagcaga agctattgaa tatgcttccg ccgacaatcg ttctgacggc
     6121 ggaagaatct catcgtcgga cggagtagag agctgaggcg caggcgccgc ctgctgctga
     6181 gggatgcggc cgatcagcaa catccccaca actaatcccc cgattaccgc tccaactaaa
     6241 gcggccacaa atagtggaaa tccaaatccg cgaggtcttc cccatcgatc gtaataaccc
     6301 atgaaatgcc acccccgtct gtcacatcta acgctatcag cagaaacaga aagctctgtc
     6361 aacctaatag tgcgttaaat tcgccgagga gagccaaaat accttcgtca gactttgatt
     6421 ctacgtatag cctcacaacg ggttccgtgc ctgacgccct gacaagaaac caatcaccgc
     6481 catcgagcgt gaatctgacg ccatctgttg tggagatgtc gcacacccgg cgccccgcca
     6541 cctcaagtgg cggtttctcc acgaatctcc taatcagagc atcccgtttg cggttgtcgg
     6601 aaaacctcat gtcgatccgg cgtgtgcaag acgcgccgta ttcgtccatg accgcccgga
     6661 gcgacttctt caagggcctg ccttcgaccg ctctgaattc ggcaagaagc aggttcgcaa
     6721 gaatgccgtc tttttcggga acgtgtccgc caatcgagag gcctccgctc tcctctccca
     6781 cgattatcac gtctctctcc ctcatcacct ggcatatgta tttgaatcct accggtgttt
     6841 cgatgacgtc gattccatga gcatgggcta tcctgtcgat caaatgagtg gtggcgatag
     6901 tacgcacaac caccccttgc ccccttccag cccgtataag atggagcaag agcagtggga
     6961 ttatctgatt tgcccccaaa tatgttccgt ctgaatcgat ggcgccaaat ctgtcggcgt
     7021 ccccgtccgt ggcaaggcca atatctgcgc ccgaagagag caccttctcc cggagatcac
     7081 ccaagctctc ctcttgagga tcgggggatc ctcctccaaa gagcggatcc ctacagccat
     7141 ttatagtctc aacgttacat cctgcctctg ccaatatcct gtctacgtat cctctgcccg
     7201 tagaaaaaag cgggtcgtac acgatcttga tgccgctttc ggcaattata tcgagagcca
     7261 caagggatcg aatgcgcgac aggtatctgt ctgaaggatc gatcgatctg acaactcccg
     7321 ccgcaagggc ctcctcgaaa ggcatcgtac gcggaatcga cccggcattc gcccgccgtg
     7381 ctatgtgtcc ttctatttca ttggtaattt ctgggtcggc aggcccggca taatgcggaa
     7441 tccacttcac gccgttgtat tcaggcggat tatggctggc agtaaacatg actgcccccg
     7501 caaggcctcg gtcggcgacg gtgaatgcca ctacaggtgt tggagtgtcg cggttggtga
     7561 caagggttgg gattgagttg gccgctaaca cttctgcggc gacctctgca aatccgcggg
     7621 aaaggaaacg ggcatcatac cccaccaaaa cgccgcggtc acgcatatca tggctgatca
     7681 cgtagtccgc caccgcctga atgacggctc ggacattggc gaaggtgaaa gcatcgcaca
     7741 ttaccgccct ccacccatct gttccaaatc tgatctcgct tcccatatga gtccctcctc
     7801 ctcatgttgc agcactattg tctcagcaca aagaaggacc ctagccgggc tttcgcggaa
     7861 tgtcggcctt cggttctctg cccgcgttcc atgccgccaa agccgagtca gtactctatt
     7921 ccatgccttg cggagattcc gcgcatgtac gggtgcttca ccatcttcat ctcagtgacc
     7981 aggtccgcca tgtcgatgag ggtctttggc atgtctcgcc ctgtgaggac tagctcgaca
     8041 ccatccggac ggcgctccac aagggagacg gcatcttcga cgctgaggag accgaagttg
     8101 actgcattgg cgatttcgtc tagtattacc agatcagccg ctttctcgtc aagcgtggcg
     8161 ccggcaagct cgtaggcctg tctcgcgtat tcgatgtcgc agggttctgg atcgcccatg
     8221 cggacgaaac aatcaccgca tccctcgcaa acagcctccc ctgatctgat cttgtcagag
     8281 tatttgcaat ccctcccgaa ctggaatatc tcgaagttgg gagcgaatgc ctttgccgcc
     8341 tccagctccc ctgtaaagct cgtccccttc atgaactgga tcatccgcac cttgtagcca
     8401 tgtcccaaag cccgcatcgc caggcccagt gcggccgtgg tcttgccttt tccgtctccc
     8461 gtatatgctt ggaccaaggc attgggaatt cggccatatg ccgcccttat ctcaccagcc
     8521 gacgtattgc attttttcac ccattgacgc ctcctcagtt cacaagtcgc atctatcatc
     8581 ccttgccgtc tccatcagag cttcccgata tcacgaaaac ggcaggccta tccaattctg
     8641 gacggcgggc cacaaccgcc ctcacgccat acacgtcgag aagattcgcc tcggtaagca
     8701 cttcatcggg gcaaccttcg gccacaacct gaccatcctg gatcatcgcc aatctgtcgc
     8761 aatatcttcc tgcgaggttc aagtcgtgga gcacggcgat agtggtaatt ccagaatcat
     8821 gggcaagcga tgtggcaagc tccattatcc ccacctggta gtttacgtcc agatgcgctg
     8881 tgggttcgtc caaaagcaac accttgggct gttgtgccaa ggccctggcg atcagcacgc
     8941 gctgcctctc accagtggaa agctccatta tgcgacgcga agcaagatgg gtcacaccgg
     9001 tgcgatccat ggcctgtgcg acatgggtaa tgtcctcggc cgactcggac tggagcggcc
     9061 tgaggtaggg tgcccttccc atcatcacga tctcctccac cgtaaaaggg aaaatgacgg
     9121 gctcccctga ggccacagca gccacctggc gtgccgcctc ccgcggcgaa agctcagaga
     9181 tgttgcgtcc gtcgtagaaa actgcaccat ggcaaggacg tgcaaccccg cctatcacgc
     9241 gaaggagtgt cgacttgccc gatccgttcg gcccgattat cccgtacaac gacccagaag
     9301 gcacgtccag gctgatgccc ttgagtgtgt caggaagatt tggcccgtag ctgtatctca
     9361 catcaacagc ctcaactctc atcttttcct ccttcggagc aggtagaaga agaacggcgc
     9421 tcctgccatt gctgtgatta ctcctacagg tatttcccgg ggaggtatta tcactctcgc
     9481 cagagtatcg gccgcaacca tgaatatagc acctcccgcc gccgaaacag ggatcagcga
     9541 gcggtggtcg gggccaatga tcatccgggc gatgtgcggg accacgaggc ccacgaaacc
     9601 gatgagcccc gatgttgaga ccacagctgc tgtgattagc gatcctatga ctatgatcac
     9661 tttcttgaat ctctccacgt ccagccccat gtagcgggcg ggttcctctc ccacagctat
     9721 gaggttgagg tcgcgcccga gggcagtcaa agccaacgtc ccacctatga cgtacggggc
     9781 ggtaattctc acatgggacc agctctttgc ggaaaggctc cccatgagcc acattaccac
     9841 tttcggcagg tcctctccgg cgacaaccat caaaaacgag accgccgccc atacaaaaga
     9901 acccacaacc acaccggcaa ggagaaaggt gtcggtggga aggcaaccat ccaaagtggc
     9961 caaggcatag acgaaagcaa ccgtgatcac tgcccctgca aaagcgaata gcgggatgta
    10021 ggaagatggc gccgaaagcg agccgatgcc cgtggcgccg gcagcgagaa tacctatgca
    10081 ggcgccgagc gcggcgcctg acgacgctcc gattatgtat ggatcggcaa gggggttcct
    10141 gaacagcccc tgaaaggacg cgcctgtggc ggaaagcccc gctcccacaa gaaggccaag
    10201 aacgatccgt ggaaggcgga tctgggtgac tattatctcc acactctcca agtcgtaggg
    10261 agcaacatcg gcctctgata ggctaacgta tcgcccaatg actggcattt tcgatagaag
    10321 caccctgagt gccaccccgg ctggcacgcc cgctgtgcca gcagacgcag accagcatat
    10381 tacggctacc atcgctaccg ccaggatcag aatgcgaagc ctcgccctgc caggtttcgt
    10441 cattagccag acccccttcc ccacatcttc ccatatcgct ctgttccaat gccccgcgga
    10501 cagcgattgc atccgtaggg actgtcccgc aaatcggttg gcgcggttgc ttgcagccta
    10561 ttttctcagc ccgaataggc tggggtggaa tatggccgcc agcgcctcaa gcccgtcgac
    10621 caaccgtggg cctggcctaa caaggaggtc gggtacaagc tcgtacacgg cgccacggac
    10681 cacggccggg attttctgcc aagcgggcct ggccatgacc tccgccttgt tgaagcatgt
    10741 cagtatgatc acatcaggct tcgacgcgac cacaagctcg ggactgatct gaggccagcc
    10801 atcaacatct cccgcaaggt tgcgtccgcc tgcgcgtgta ataaggtcat caatgaatgt
    10861 cccagctccc gcagtcatca acggatcatt ccatatctcc acaaacgcaa caggcctctt
    10921 cgactcgggc agaccggcaa cagccttttc caccgcagac actcgcgttt caagggacga
    10981 tacgaccttc cgggcccgca cgccctcgcc tgccgcggcc ccgatcatct ccatcgatct
    11041 gaaaacctca tccatggttt tcggggcgac cgctagcacc gtgatcccga gctccctgag
    11101 tttttccaca acaggcatct gcaagtcaga ctgagccaca actaagtccg gcctcttcgc
    11161 caccaccgcc tctacgttga tggtgtaatc gccgattttg tcgatcttgg cagccgcctg
    11221 cgggtaatca catgctgtcg tgaccccaac aagcctatca ccaagcccga gactgaaaag
    11281 gatctcggtg ttgctcggag caagcgacac gatcctggca ggcgccttct tgatcacgag
    11341 tctgtatccg gtgtcatcta ctatcgtgac gggaaatccg cttgacagag tggaagcagc
    11401 ataatccacc ccgtccgcgt gcagcgacgc ctccagaggc actggcgaaa acgccaggat
    11461 aacaacgaga gcaagcacga tgcttgccat tgtccgtttg ataaatgacg atctcattgt
    11521 tggacactcc tttccggttt ggcgcatgtg cgcccgtata ggccggcgtg agccgcagca
    11581 tctcgttgcg cataaaaaaa cctgttcacc catgatgaac agggacaatc ttcacgcacg
    11641 gcacaggttg cagcggtcaa ccgccactgc gccatccgca tcctcccttt tcctcgaggg
    11701 cggttacggc aagcttagtg ggcaggtttc ctggcttccg gttcatagct tcggcgcgtc
    11761 ttcccatcgc atgagcgaaa gtgacatgcg gctcgcatca gctccccggc tacagtggcg
    11821 ggaccgcggg cttcgcccgg cgcattctcc catgcccggc gcctggctgc cacttccctt
    11881 tcaacccgca tagcaggcac ccaccagcct tctatgttca gttcgctgcg tcaattctag
    11941 cacaccacat acaaacatcc aagaccgcaa ggctcaacct gccaagacgc gttcatcccc
    12001 tacccgcaca gtaatcctgt tggagtcgag atactcgagg agagccaccg catacttccg
    12061 cgtatttccg caagcatccc tgaactgggc ggcggtgaat ggcttgccac cttctccgag
    12121 actgcgcgct acttccaccg ccctggacag tgcatcccgg tggaacaaaa gctcagggcc
    12181 cactcttacc agtattccct catcgatcag gtagtcgagt atggcctccg aggtttcgcc
    12241 ggcgagcgcc tggtcccgtt cggggggaga catcaatgaa gaagaatacg ccgcttcgag
    12301 cctctctgcc tcatcccgct gctttgggct gagtgagaca gtgtgccaag gcagtgccac
    12361 caaagtgcct cgttcttcga tgagcccgtc tcttgccgca gctgcgatca gcatcgaaaa
    12421 tggcttgcca tcccaagagg acatggtcct cgttcggagc tcttccttcg ccatccctgc
    12481 ccgtaatggt tcgcggcgat gatattcgga aagtgccttg ataatggcat ctgtagcctt
    12541 gtcccaatca tcccgtggca tatacgtgcg ggtgcccggc gattcgccga aggccacagc
    12601 ccatccgaga gccactcctt cttcgaagat atcgcgcacg tgctctggat ctgcgcacat
    12661 cgaccgggct acgtcgaatg cagacgcagg cgcagacttc tgggcgatga tggaggaagc
    12721 aaagagtcgc gcggtggcgg attcgtcatc tcccgacaac gtctcaaacc aatgcagcgt
    12781 ctcctctttg aaccggcgct gacgccctct ggacaccgcg agcacgcggc ctcctccggc
    12841 cgtcttcata ggagagtaag tgcggactat gaatcggtcg ccgcgggtcg cggcaatctc
    12901 ctcctccagt tctagttgga caaagacgcg gtcgcccggg catgccccat cggtcccgat
    12961 cgtcctcact ctgcaaacag cctccgctgc accgatgtga aggtgcatcc tctcgccatg
    13021 ttcgatcaca tccgctgatt cgaggagagt aagctcggca tcgaatatcc gagcaggctc
    13081 gatcattcca ggcttggcca gaaccatccc gcgctctatg ttggcacggt cgatgccagc
    13141 gaggttgaga gccacgcgtt gcccgcgcac ggcttcatcc acagcactgt cgtggacctg
    13201 tacttgccgg acccggacgc tcttacccgc gggaagaagc tccaacttat cgccgggctt
    13261 cacctctccg cccgtcagcg tccctgtaac cacggtgccg tgtcctgaga ttgcaaaaac
    13321 tcggtctatg gggagcctgg tgaaactcca ctcctcttgc agcacagcgc ggccaactgc
    13381 ggaatctatc gcggcaagga gcgtgtcgag gccggtgcgt gtggcggccg atacacagat
    13441 tataggggca tttgcaagaa aagtgcttgc caggcgttcc ttaacgtctt caacaactag
    13501 ctccatccat tcatcatcgt ccaccaaatc cgccttggtt atagccacaa cgccctcgcg
    13561 cacgcccaga agctggagta tatcgatatg ctcttctgtc tgaggcatca cgccttcatc
    13621 ggccgccact accagcaaga ccacgtcgac gcctgtggcc cccgccaaca tgttcttgat
    13681 gaaccgctca tgcccgggca catcaacaac gcccgcaacc cgcccggaag gcagcatgat
    13741 cggagcgaag ccgaggtcga tcgtcatgcc cctctcctgc tcctgtcgga ggcggtcggc
    13801 atcgacccca gtcagcgcct tgatgagcgt ggtctttcca tgatcaacat gcccggcagt
    13861 gccgattatc gcatatctca tcagtcttgc tccttctcgc tgaagtcaag atcagtatcg
    13921 ccaccgaaag ttgccggcga agccaggata tcacacacgt gctcggccag aatcttcaag
    13981 tctctttcga aaagcgttct catgtgaaaa cgaacagagc cttcggcaat gcgcacaacc
    14041 actggcggtt cgcatcttct gaggcgcgtc tgcagctggt cagcgggaat acagcgggat
    14101 gtcacggaaa cgcttgcccc tgggatagac acggtaggca tagagcctcc tcctgcctcg
    14161 gacgggtcat cgactatcgc aaggtctacc aaattccgag gtatctccct ctcgtcgagt
    14221 ccggcctcga tcagccccat gagcgaagca gcccgctctc tgagttcatc tgtgttttgg
    14281 gtgagcatgg ataagaccgg tatggattcc cctgcgtcat cagggttctc ccatgccgcc
    14341 gccacagcag ccagggcaga caaggtaagc ttgtctatcc gcagagcccg cgcaagcggg
    14401 ttgacagata tcttgcggat cagatcttcg cggccgagga tgatgccggc ctggggcccc
    14461 ccaaagagtt tgtcaccgct gaacgtgacg agatccgccc cggatgccac cgcttctatg
    14521 acagtaggct cctccgcgtg cagaccgaat cgtccgagat cagacacaag acccgaacct
    14581 aagtcataca tgaccgggag cccgcactct cttcccagtt ctacaagctc cgccagctgc
    14641 acctcctcag taaatcccat tacccggaaa ttcgaggtgt ggactttgag gagaagccct
    14701 gcctctgaat cgatggcgcg acggtagtcc gaaagacgag tgcggttggt cgttcccact
    14761 tcgaccaggg ttgctcccga cctttccatg atgtcgggga ctctgaagga tccgcctatc
    14821 tccactagct cgcctcggct taccagcact ttgcggccac gcgccaacgt gtcaagacac
    14881 agcaggactg ctgcagcatt gttgtttact acataggctg caggcgcacc cgtaagctct
    14941 gccatggcgc gggctatgtg atcctgtcga gagcctcgtc cgccgccttc aaggtcatat
    15001 tcgagatcag aatagcccat tgcgcattct gccacggccc gcgccgcata ttccggaagc
    15061 ggagcgcgcc ccagattggt atggaggact attcccgtgg cgtttatcac atacctaggc
    15121 cctggcgctg cagccaagac ggttgcagac ggcaggttcg ccacaacacg ggccaatacg
    15181 tcctgccttt gcacatctat ggagcagacc ccttggccgg ccacggaaga aaacctccgc
    15241 ctctccgaat ctataagctc acgggcggca ttccgcacag cccacctggg atacgccgcc
    15301 atcagttcta cggcggcagg atcggaaagc acatccgata ttgacggaat cgcgcgcatc
    15361 aattcgttca attcatattg cacctgccgt tcacaaagta tctcggcggg tcaggagacc
    15421 accgttatcg ttgggccctc cggaccccgc cggcacgctt ctccaactat cgcggccgcc
    15481 aaggcgcccg cgtcctttaa tcggcgcaca agctcatctg ctttgtcaga cgccagggcg
    15541 atgaggagcc cgcctgatgt ctgcgggtcg cacatcgcat caacgatatt ccggtcaaca
    15601 ttgcccccca cctccactac gggcccaaca tgctcccaat tgcggtgaag gcctgcaggg
    15661 gcgagtccca tatctatgta gtcaagtgcg ccgtcaataa ccgggacggc atcggcatag
    15721 acagtgaatg acacgtctga cgcatctgcc atctccgcaa gatggcctag aaagccgaag
    15781 cccgtaatgt cggtgcacgc atggacaccg atctcctgca tgacagcgga cgcgctggca
    15841 ttgagcgtgg acatcacagt ataggcgtaa cgctctacat gagggtccac catctcgcct
    15901 ttgagggcgg tggaaatcac gcctgtaccc aaaggcttcg taaggacaag gacatctcca
    15961 gggcgcgccc ccttgttgag caccaccttg tcggggtgga ccgttcccgt cactgaaagg
    16021 ccatacttgg gctccttgtc atccacggag tggcccccaa ccagcaccgc gccggactcg
    16081 tatactttgt ccgctccgcc ccggaggatg tctcgaagaa cctccatccc aagcgagcaa
    16141 ggataacata caacgttcat agcgactatg gggacgcctc ccatggcata cacgtcagag
    16201 agagaattgg cagccgctat ttgaccaaat tcatacggat catccactat cggggtgaag
    16261 aagtcgacag tctggatgat ggctatgtcg ggagtcagct tgtagacgcc ggcatcttcc
    16321 gatccgtcga accccgcgat aagcagcgga tccttgcgga caacgagccc tctaacagcc
    16381 atctgtaggt cacccggacc tatcttggca gctcagccgg cttttgttga cattgtggta
    16441 agtctgatgc gatcagtctc tgtcaccttc tgtcactcct cgcaaaacaa acttgaaccc
    16501 ataccctact ctagtttacc cgcgccagtc tgtccatctc ttgggcaaat aggccggtgt
    16561 gccacggacg cgcatcgagg accgcgggga tcagctagtg gggcacattg ggcatgaaaa
    16621 tggcgggagt acgtgggaat cgaacccacc gcggccaggt tagctgcccg ccactggatt
    16681 tgaagtccag gaggcccacc aggaccaatc tactcccgcg tgaaagactg agatgtgtat
    16741 gttcccatta taaccgtaca tcctttcctc gtcgaggggg caccgaatct aagcccttac
    16801 gcgctgtccc gttttgcgat tggcaaaggc cgaaaatgcc aggctggtgc cgagatatct
    16861 gtgcggagca aactgcctgg gtcgcccggg gtgattccgg caatcctcct taacttccat
    16921 gccagaatcc ttgtccccaa cgcattctta tacgcattag catgacttga gtaggtctcg
    16981 acaagtctct gcacgattcc ga
//
LOCUS       DAOSYL010000051        16026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3038_length_16026_cov_8.291528, whole genome shotgun sequence.
ACCESSION   DAOSYL010000051 DAOSYL010000000
VERSION     DAOSYL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16026
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_16026_cov_8.291528"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..616
                     /locus_tag="PLU15_08290"
     CDS             347..616
                     /locus_tag="PLU15_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30958.1"
                     /translation="MIADTPHERMLLQQMLVDACATDEMLDVGALLMKLVVKDRAFMK
                     YGADAVVAVREAPLDFREMVMEAGWHGVEALDSRVDEIVDALVGD"
     gene            complement(963..2978)
                     /locus_tag="PLU15_08295"
     CDS             complement(963..2978)
                     /locus_tag="PLU15_08295"
                     /inference="COORDINATES: protein motif:HMM:NF018528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HOO30959.1"
                     /translation="MKPAAERILTTPSELGDIVSPYVTGNEWVALPDIFPSDASLGSI
                     NIVRMASKGLVEWVGEFEEDQDRRNEPKAPFLRPFVNAIGASGKPAETESVGDIEWTW
                     LGHWIPVGKCRLRTKEGHECVAKIAFCAPVGERGFMVALELSELEARNGASHVQFHLG
                     LEGNWGATVSTVFGSRLMHVTNHAYYSPWTNSLILEARGTSSVGALAFSADSQFDWAI
                     WASKDGVDMKESPVLAVPNGSADIRFRLSSSMTLAANEHRTVAFWIAANADPDGAGTT
                     NVHLRRVGAEAALGNTLSWLEARAHSSRVSPVEIGEADEEILARADRNLHFCRFFAHG
                     KAIDTEELVMLTSRSYRYYVSAVFWPRDTFLWAFPAMLASDHVFAREVLTEGFRRHVR
                     NMGTHAHYIDGVLLYPGFELDQLASYVIALGTYIEATGDASLADKAVIADGLDQFDLV
                     LAAQRHPDVALYKTFLDPSDDPVVYPYLTYDNVLAWRALRILSEIARNQGDDYRAERS
                     AAMAQATESSILEHCIAPGPFGQMFAWSTDLAGNYELYDDPPGSLVLLPYHGFVDVDN
                     ECYANTLKFIRSDHNPYACSLGTYKGAGCAHSKHPWPMHACNMILAGVADADALHLIE
                     NAPLDGGLACETVYASTGKAATGSAFATFAGYLSLAAFTCFRQNVGQ"
     gene            complement(2984..3706)
                     /locus_tag="PLU15_08300"
     CDS             complement(2984..3706)
                     /locus_tag="PLU15_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOO30960.1"
                     /translation="MRHKATVVCAHPDDLEYFCGGAVSVMIRDDWDICLIIATDGDKG
                     TQDREMSAARLASERRHESMKAAKIVGISKVFFLGKEDGELRSDNSLRPDITRILRSE
                     QPERLITFDPWRKYELHPDHREIGFAALDARLAARLPLYYPEQLRDGLENWTIREVLL
                     FSTDNPDYFVDISDTFDLKLQALRAHKSQWETIWQETRSDLLHEAERMGAMAGCELAE
                     GFKRIYIPEGPVSIKFEHERGD"
     gene            complement(3707..4882)
                     /locus_tag="PLU15_08305"
     CDS             complement(3707..4882)
                     /locus_tag="PLU15_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOO30961.1"
                     /translation="MAQLIDSIRSRAMNIALSRASDPKAVSRRASWLKEGDARFDGRQ
                     PTPFATRHGKDAKPERVICAAIQMKAKTVSSGTEYVELVYRLAAKAAVAGASIVVFPE
                     YSGLPLLGMAPGMRKKLDSASSLDEAVTEAGGPDAGVLDVFKLVGPYAERAYKEAFSS
                     VARELGVYIISGSMVLSDGSTDGSSMRAIGYVFGPQGELVGRYSKSHLMPIEAQWGIS
                     PGNSIPTFNLGGVIVSVPICMDATYFETFRIARFAGADIVAIPSANPEPYNLWYALRG
                     IWPRVQESQVYGIGSSMVGSFAGQDFTGRSSLLAPLELSPGGDGILAQAMTYNEEDVV
                     VAEFDLPLLYKLRAEDPLRFNLALYRKYLPGMYRAAQSSRRAGDAGEARDENKGETC"
     gene            complement(4889..5803)
                     /locus_tag="PLU15_08310"
     CDS             complement(4889..5803)
                     /locus_tag="PLU15_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOO30962.1"
                     /translation="MSTVLSCGLLQMDPAQFKTVHDFQVSIAARISSLARRGAELVVM
                     PEYAAMIGAGLETAPHAPGNMFDVARLTGAEGYHRWLDFFAATALSNRVHLCPGTFIV
                     PSDGGYTNSCPFFSPRGDLIAEQVQTHLSEQEDRMGLVCGDDIATFNIHGHKLGIAVG
                     TDAWYPEVGRILALLGVDTILAPTAVPAPYTVWRQTRGMWQIVQQNQVFGVEASLGGE
                     WLGVSYQGRSRIFAPVEVTPEGRGVLAEMTSGSIWEELTATLDPSILAEARRTFPIFE
                     HFNVELYENQLSDAYLVGRTVRLKQGRR"
     gene            5927..7312
                     /locus_tag="PLU15_08315"
     CDS             5927..7312
                     /locus_tag="PLU15_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOO30963.1"
                     /translation="MYSRPEAKPQPARALSIIFIFLALSTILVGAITDFVVTEVMSSR
                     LNRAFQAEVENAAVTWPEDLPTGLDYGSKSVPSDCKRDLAALQPDRPRRVRALYATGW
                     TAGSGSRMKQLVDLVCDTELNSIVIEIKDDLGVISYRSDVPLATEIGASTGKIADLRR
                     LLDELYDSGVYPIARLVVFKDPQLAAARPDLAVRDSSGVPWHDSQGLMWTDPRKKEVW
                     EYNLAIAREAARAGFREIQFDYVRFPDDAGLARTNWLDLEGKNRVDVISDFLDYAGRE
                     LEPYKVVITADIFGIVCSSRSDMGIGQKLEEMAKHVDYLAPMVYPSHYRKGEYGIANP
                     DAAPRETVKRSLSDAQDRIAKAGGRAKIIPWLQDFSLGYRYTAAEVRAQIDAAAECGI
                     YEFMLWNPRNLYTAGALGRPDPEDALRPRPHLDLLAGSGAIRLEDPVTVTEWRALSAL
                     KQAGVGQILGP"
     gene            7436..9244
                     /gene="aspS"
                     /locus_tag="PLU15_08320"
     CDS             7436..9244
                     /gene="aspS"
                     /locus_tag="PLU15_08320"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOO30964.1"
                     /translation="MSYVLRTHTCGELAAGNVGETVALCGWVQSVRDHGGLIFVDLRD
                     RYGRTQVVVSPDLSEDILERARSIHRESAIRVEGIVSERPEGTKNPSISTGDVEVMAS
                     NIEIIGDAAPVLPMEVSDENLANEEVRLRYRYIDLRRPSLQANMMARHRAAMAVRNYL
                     SDQGFLEIQTPLLVRSTPEGARDFVVPSRNFPGKFYALPQSPQLYKQLLMIAGFDRYF
                     QIAPCFRDEDQRADRQLVHSQIDIEMSFCDEEDVFSVVEGIISAAFKGAIGADVKGPF
                     LRMTYDEAMERFGSDKPDMRFGMELVDLTDLVADSEFGIFKSVCATGGRVRGISAPGC
                     AGFSRKQIDDLTELARIYRAKGLITLKVEGGEIQGSVAKHISSDTLGEIASRLGSADG
                     DLILIIADTPEVSATALGHLRRYLGKELGLIPKGDYKLLWVTDFPLFEWNPDDGRWDP
                     MHHIFTMPREGDLELLESDPASVKGRQYDLVLNGTELGSGSIRINDPAIQKRVMNVIG
                     VTPKEAEEKFGFLLRAYQYGGPIHGGFAIGFDRLLAVMLGLESLRDVIAFPQNAAGVS
                     LVDDCPNEIDPKQWKELHLKLEDCAGKPDAEARSDC"
     gene            9378..10856
                     /gene="guaB"
                     /locus_tag="PLU15_08325"
     CDS             9378..10856
                     /gene="guaB"
                     /locus_tag="PLU15_08325"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOO30965.1"
                     /translation="MHKPDKIREALAFDDVLLVPGISEVLPANVDVRTEITDKIMLNI
                     PLMSAAMDTVTEARMAVALAREGGMGIIHKNMSIEEQALEVDKVKRSEHGIIVDPIFL
                     SPEASVREALEIMERYHISGVPITEDGTLVGILTNRDLRFETDYDQPVSNVMTKDGLI
                     TAPVGTTVDEAIEILKVHKVEKLPLVDEKFKLKGLITIKDIKKTRKYPNAAKDEHGRL
                     RVGAAVGVSVGTMDRVAALIDAGADMIVVDTAHGHSRGVIETVKAIRTNFGDIDIMAG
                     NVATGDGVKALAEAGANCVKVGIGPGSICTTRVVAGVGVPQFSAILECAAAAAEAGIR
                     IVADGGVKFSGDMTKALAAGAHVVMVGQLLAGTDESPGDTEIYKGRSFKVYRGMGSIS
                     AMKAGSSDRYFQEGAKKLVPEGIEGRVPYKGSVSDTVFQLVGGVKAGMGYVGAANLEE
                     LREKATFVRITNAGLRESHPHDVHITKEAPNYMIAGAEYEES"
     gene            10888..12096
                     /locus_tag="PLU15_08330"
     CDS             10888..12096
                     /locus_tag="PLU15_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOO30966.1"
                     /translation="MSTKIKRCSLLAVSLIMVMVVSMISSESALAEYDVKSIIINPNP
                     PYQTSIWTDKSQYNPGERLNVYFRTSKDAYIYIFDIDTVGNVSLIFPNIYSEYNYKRA
                     GQTYSLPDNSRYNLTIGGPAGVEQLVLIATPSQIRDVEWLRRSLEQGSFGPQLNVNIS
                     ADRFMFEVKSISITPNFGQDWSSAVATFTVGSVVTPPPPPPSVKYGTLSITSKPSGAT
                     VFLDGVDQGTTPLTITGVSYGVHDIAIMKKNYYTYSRQITVNSPNPYRIDATLVKLPT
                     SSDWGDEVQLVNKSMLVKWPQTGPFTETFTFRGSTGKVVINADPILGMLSRVTGSITA
                     NAVGPVEFMQINPSGPDAPWPGRVYERIQYPFRVRVTVEDYSITTGLVFGIEYIDYVR
                     LRLEIWHIGN"
     gene            12835..14127
                     /gene="asnS"
                     /locus_tag="PLU15_08335"
     CDS             12835..14127
                     /gene="asnS"
                     /locus_tag="PLU15_08335"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOO30967.1"
                     /translation="MQSVRIADIREHDGEEVTIKGWLYNKRSSGGIHFLIIRDGTGFM
                     QAVAVRGEIPDDQFELCNNLTQESSILVTGRIRADKRAAGGFEMSATGVELVQLAEEY
                     PITLKEHGVDFLLDHRHLWIRNPRQHAALLVRDEVISAAEDWLRSNGFVKIDAPILTP
                     AACEGTTTLFETDYFGEKAYLSQSGQLYNEAACMAFGKVYCYGPTFRAEKSKTRRHLI
                     EFWMIEPEMAYATQEDNMVIQEEFLSYIVQRCLAKRRQELITLERDLSKLECIVPPFP
                     RLSYDEAVELLHGEGKEFEWGDDFGAPDEATISMKYDKPVFVTGYPTKVKAFYMQPDP
                     DRPEIVKCSDLLAPEGYGEIIGSSERIHDLDLLAARMREHNLPEEAYGWYLDLRRYGS
                     VPHSGFGLGIERTVAWITGSEHVREMIPFPRLINRIYP"
     gene            14266..>16026
                     /locus_tag="PLU15_08340"
     CDS             14266..>16026
                     /locus_tag="PLU15_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO30968.1"
                     /translation="MKSKRKVLFIFASLALVLFVGTLVSTSSQAQISAYDYILRGEQY
                     LAQGAANEAIGQFEQAIYIEPTNTRAHFGLGCAYEMRSKAARVFNYRMELFTEPDSVR
                     KLGFPPLSEQGYQDVERAIQAYSKAALYSPPYAEGHYRQGVLHFISGRPGDAKVALER
                     AVAQDRTAVDPAVLLAYIYLEEGNIQAATAQIDRIISATGDRLNPVILGVSAVLEYRS
                     GRYDQAEQLLLQATSRSGAPAYLYKRLGDAMAARGLHTQALDAYRQAVQRDPESILAN
                     DAIGNIYRLSGQSAQAVQCYSAAASANPNLLRARYGLGSALLESGSYAQALQTFSELV
                     DALPTYGSDNALLLKGIAQWKTGNANAALATVNHALGINAENRIAAIYKERIEREMGG
                     YGVSPLGVQPKSIVAEGSKPDKPFSGIVINDGAEYTNSRTVRLAVYSDYSTVAFSNDN
                     STWSSWFSKPEPYGEFTWELTPGDGTKRVYVKFRGLFGIGIPGESGTIVLDSTPPVAT
                     ARLESMQTGGYGGYDGRTRVVLSANDPSGIVGFWMSYDGNDWRWFDWYGSDFAISLPA
                     GVGATPRVRFSVVDGAGNHAS"
BASE COUNT         3664 a         3981 c         4728 g         3653 t
ORIGIN      
        1 ccggattctt ttttatcttc aagagcctcg gcgtattgcc ggggctcttt gctttattat
       61 gggtgcagtg gattttcacg gggttacctt tggtcctttg agtggctcat aatccatttg
      121 ggcgtgggat gagaagtcat gagcccgttt ttagcagggc gtggagggct gtgataggac
      181 aggcgagccg tgctagccaa gaaatggagc atatgtcagg gaaaaggctg caaatagccc
      241 ataaggacga aataacgtgg atttaccaaa gataacgaga ctttacgatt acaagacagg
      301 atgataacat gggaatacaa ggcgatgctg ggaggaggtg gggctagtga tcgccgatac
      361 gcctcatgag cggatgttgc tccagcagat gcttgtggat gcgtgtgcga ccgatgaaat
      421 gctggatgtt ggtgctcttc tcatgaagct cgtagtgaag gacagggctt tcatgaagta
      481 tggtgccgac gcagtggtgg cggtgcggga ggcgccgctt gattttcgtg agatggttat
      541 ggaggcaggg tggcacgggg tggaagccct tgatagcaga gtggacgaga tagtagatgc
      601 gctcgtgggg gactgaccgg gcaaggtggc gctagtgatc agctggcatc ggggaagtac
      661 gaagcatctt gacttgcttc gggttggctc ttgcgaagaa ttcaccgtgg tccgtctgtc
      721 atgagaatga tcggcaaaat cacattggcc gtactgtgcg aagctgctac tttggcgggc
      781 cattagattt gtgggcattg ccatcaatgt tctgtaggct gccgcgaggc attcggcgtg
      841 gtcgatagcg ggctttcttt tgaatgtagg ccgacaggac ggcatcatac gctttgatat
      901 tggcaccttc gttgtgggtg gccccaagtg tttaccggct gtgtgaacaa gcctccaagc
      961 gcctattggc ccacgttttg tcggaagcag gtgaacgccg ccagcgacag gtaaccggca
     1021 aatgtggcga aggcactgcc tgtagcggct ttgccagtag atgcgtatac ggtttcacat
     1081 gccagccccc cgtcgagggg ggcattttct attaggtgta atgcgtcagc gtcggccacg
     1141 ccggccagta tcatgttgca cgcgtgcatg ggccacgggt gtttcgaatg ggcacagcct
     1201 gctcccttgt aggtccctag ggagcatgcg taagggttat ggtctgacct gataaatttg
     1261 agggtattgg cgtagcactc gttgtcgacg tccacgaatc cgtggtatgg aaggagcacc
     1321 agactgcccg ggggatcgtc atacagctcg tagtttccgg caaggtctgt cgaccatgcg
     1381 aacatttggc cgaaaggccc tggggcaata cagtgctcga gtattgagga ctctgtcgcc
     1441 tgtgccatgg ctgcgctccg ctccgcccgg tagtcatctc cttggttccg cgcgatttca
     1501 gacagtatgc gcaaggctct ccaagctagc acgttgtcgt atgtgaggta gggatataca
     1561 accgggtcat cactggggtc taagaatgtc ttgtaaagcg ctacgtctgg atggcgttgg
     1621 gcagcaagca ccaggtcgaa ctggtcgagc ccatcggcta taacggcttt gtctgcaagc
     1681 gatgcatcgc ctgtcgcttc gatataggtt ccaagggcga ttacgtatga tgccagctga
     1741 tcaagttcaa agccgggata aagcaggacg ccgtctatgt agtgggcatg cgtgcccatg
     1801 ttccgcacgt ggcggcgaaa tccttcggtc agcacttcgc gggcgaatac gtgatcggag
     1861 gcgagcatcg ccgggaatgc ccagagaaat gtgtctctcg gccagaaaac cgcagacacg
     1921 taatagcgat agctcctgga tgtaagcatg actaactctt cggtgtcgat agcctttccg
     1981 tgtgcaaaaa acctgcaaaa atgtaggttg cgatccgccc gcgcgagaat ctcctcgtct
     2041 gcttccccta tctcaacagg cgaaaccctg gatgaatgtg ctctggcctc aagccatgag
     2101 agcgtgtttc ccaatgccgc ctcggcgcca actcgccgaa gatgaacatt tgtggttccc
     2161 gccccgtcag gatcggcgtt ggcagcgatc cagaatgcaa ctgtgcgatg ctcgtttgcg
     2221 gccagggtca tgcttgagga gagacgaaag cggatatcgg cggagccgtt cggcacggcc
     2281 aggaccgggc tttccttcat gtcgacgcca tctttgcttg cccagatagc ccagtcgaac
     2341 tgggagtcgg ctgagaatgc caatgcaccg acgctcgaag ttcccctggc ttcgaggatg
     2401 agtgaattgg tccatggtga ataataggcg tggtttgtga catgcatgag cctgctccca
     2461 aacaccgtgc tgacggtggc gccccagttc ccctcgagcc ctaggtgaaa ctggacatgg
     2521 cttgcgccgt tgcgtgcctc aagctctgaa agctctaagg ccaccatgaa gccccgttcg
     2581 cctacagggg cgcaaaaggc gattttcgca acacactcat gtccttcctt tgtgcgaagg
     2641 cggcacttgc ccacggggat ccagtggcct aaccaggtcc attcaatgtc gccgacggat
     2701 tcggtttcgg ccggctttcc cgaggctcca attgcattca caaacggtct gaggaacggc
     2761 gccttcggct cgttgcggcg gtcttgatcc tcctcaaact cacccaccca ctcaaccagc
     2821 ccttttgagg ccatacgcac tatgtttatg ctccccaaac ttgcatcgga gggaaagatg
     2881 tcgggcagtg caacccactc gttgccagtg acgtatgggg agactatgtc gccaagctcg
     2941 gatggagtag tgagtatgcg ttcagctgca ggcttcatat ggatcaatct cctcgctcat
     3001 gttcgaactt gatgctgacc gggccttctg ggatgtatat ccgtttgaat ccctcagcaa
     3061 gttcgcaccc ggccatggct cccatcctct ctgcctcgtg cagcaaatca gagcgggttt
     3121 cctgccatat ggtttcccat tggcttttat gggcacggag tgcctgcaat ttgagatcga
     3181 atgtgtcgct tatatctaca aaataatcag ggttgtcggt gctgaacagc aggacttctc
     3241 tgatagtcca gttttcgagc ccgtctcgaa gttgctctgg ataatacagg ggaagccttg
     3301 ctgccaggcg cgcatcgagc gccgcgaatc caatttctct atggtcaggg tggagttcat
     3361 atttgcgcca gggatcgaat gttatcaggc gttccggctg ttcactcctg aggatgcgcg
     3421 tgatgtcggg tcgaagggag ttgtcggagc ggagctctcc gtcttccttg cctaggaaga
     3481 agaccttgct gatacctacg atcttggcgg ctttcataga ttcgtggcgg cgttcggagg
     3541 caagccgggc tgcggacatc tccctgtctt gtgtgccctt gtcgccatcg gtggcgatta
     3601 tgagacatat gtcccaatcg tcacgaatca tgactgagac agctccgccg cagaaatatt
     3661 cgagatcgtc tgggtgagca cacactaccg tagctttatg tctcatttag cacgtctctc
     3721 ctttgttttc gtcccgagct tcacctgcat ccccagccct ccgtgaagac tgggctgcgc
     3781 ggtacatacc aggcagatac ttgcggtaca gggcaagatt gaaacgcagc ggatcttccg
     3841 cccgaagctt gtacaagagc ggcaggtcga actctgcaac taccacgtcc tcctcgttgt
     3901 acgtcatggc ttgcgccaag atgccgtctc ctcccggcga aagctccaac ggcgccaaga
     3961 gagaacttcg cccggtgaaa tcttggcctg caaaggatcc caccatagat gaaccgatgc
     4021 cgtagacctg ggattcctgt actcgaggcc atatgcctcg caacgcgtac cataggttgt
     4081 atggttcggg gttcgcggaa ggtatcgcga cgatgtcggc tccggcgaac ctggctatcc
     4141 ggaacgtctc aaaataggtt gcgtccatgc atatggggac ggatactatg actccgccca
     4201 ggttgaatgt ggggatggag tttcctgggc tgatacccca ttgggcttct atgggcatga
     4261 gatgcgactt gctatacctg cctaccagtt cgccttgggg cccgaataca tagcctattg
     4321 cccgcatgga actgccatca gttgaaccat cggaaaggac catgcttcct gagattatgt
     4381 agaccccaag ctcgcgagcg acagaagaga aggcctcctt ataggcgcgc tccgcatagg
     4441 gccctacgag tttgaataca tcaagaacac ctgcatccgg gcctccagcc tcagtgacgg
     4501 cttcatcaag agaacttgct gaatccagct tcttgcgcat cccaggcgcc atacccagca
     4561 agggcagacc gctgtattcg ggaaatacga cgatcgaagc tcctgccacc gcagccttcg
     4621 ctgctagcct gtatacgagc tccacatact ctgtccctga agagaccgtt ttcgctttca
     4681 tctggattgc ggcgcagata acgcgctcgg gcttggcgtc cttgccatgt ctggttgcga
     4741 agggggtcgg ctgacgaccg tcgaatcgcg cgtctccttc cttcagccac gaagcccgtc
     4801 gcgagacggc tttgggatcg gaggcccgcg aaagggcgat attcatggct ctgctgcgaa
     4861 ttgaatcgat cagttgcgcc attttcggct acctccttcc ctgtttaagc cgcactgtac
     4921 gcccgacgag atacgcatct gacagctggt tctcgtacag ctctacattg aagtgttcga
     4981 aaatgggaaa ggtccgtctc gcctctgcca agatcgatgg atcaagggtt gccgtcaact
     5041 cttcccatat cgaccccgag gtcatctctg caagtactcc ccggccttca ggggttactt
     5101 cgaccggcgc aaatatgcgt gatctcccct gataggaaac accgagccac tcgcctccga
     5161 gggaggcttc aactccgaag acctgattct gctgtacaat ctgccacata ccgcgcgtct
     5221 ggcgccatac ggtgtatggg gcgggtactg cagtgggagc cagtatcgtg tctactccga
     5281 gcagcgcaag tattcttccg acctccgggt accatgcgtc ggtgccgacg gcaattccca
     5341 gcttgtggcc gtgtatgttg aaagtagcaa tatcatcgcc acacactagt cccattcgat
     5401 cctcttgctc cgaaagatgg gtctgcactt gttctgctat gaggtcgccc ctaggcgaga
     5461 agaatgggca tgaattcgtg tagcctccat ccgatggaac aatgaaggtt cctgggcaca
     5521 ggtgtacgcg attggatagg gccgtagccg caaagaaatc gagccatcta tgatatccct
     5581 cggcaccggt caaccgggca acatcgaaca tgttcccggg ggcgtgcggg gcagtttcaa
     5641 gcccggcgcc tatcattgcg gcgtattcag gcattactac aagctcggct ccgcgtctgg
     5701 ccagagatga tatccttgcg gcaatcgata cttgaaaatc gtgaaccgtt ttgaactggg
     5761 ctggatccat ttgcagcaat ccgcaggaaa gaacagtcga catgcaggtc acccccagaa
     5821 gaagtattac acattatcca cactgttcct gcaccgacga gaaggaacgg aaagctgtga
     5881 gtagaatgaa aactcgggtg ggtggaaggg agagtataca ctgtatatgt attcacgtcc
     5941 ggaggcgaaa cctcaaccag cgcgtgcgct gtccataatc ttcatattct tggcactctc
     6001 gacaatacta gtcggtgcca tcactgactt tgtcgtcaca gaagtcatgt catcaaggtt
     6061 gaaccgggct tttcaggccg aagtagaaaa tgctgccgta acatggccgg aagatcttcc
     6121 gacgggtctc gattatggaa gcaagtccgt gccttcagat tgcaagcgtg accttgcagc
     6181 gctgcagccc gacaggccta ggcgcgttag ggctctttat gctactggct ggacggctgg
     6241 aagcgggtcg cggatgaagc agcttgtcga tcttgtgtgc gacacggagc tgaattccat
     6301 tgtcatagag ataaaggacg accttggagt gataagctat agatcggacg tgccgcttgc
     6361 gacagagatc ggagcgtcta caggcaagat cgcagatctt cggcgcctgc ttgacgagct
     6421 ttacgatagc ggggtttatc ccatagctcg cctggtcgtg ttcaaggacc cgcaacttgc
     6481 ggccgcccgt cccgatcttg ccgtgcggga ttcttcgggc gtgccctggc acgacagtca
     6541 aggcttgatg tggacggatc ctaggaagaa agaggtgtgg gagtacaatc tcgcgatagc
     6601 ccgggaggcg gcgcgagccg gcttccgcga aatacaattc gactacgtga ggttcccaga
     6661 tgatgctgga ttggcccgca ccaattggct agacctcgag ggcaaaaaca gagtcgatgt
     6721 gatttccgac ttccttgatt atgccgggcg ggaacttgaa ccatacaaag tagtgatcac
     6781 cgcggacatt ttcgggatag tctgctcttc tcgatcagac atgggcatcg ggcaaaagct
     6841 ggaggagatg gcaaagcacg tagactattt ggcgccaatg gtgtatccgt cgcattaccg
     6901 caagggcgag tacggcattg cgaatcccga tgcggcgccg agggagactg tgaaacggag
     6961 cctcagcgat gcacaagaca ggattgcaaa ggcgggcggc cgggcaaaga taataccatg
     7021 gctacaggat ttttccctag ggtatcggta tacagccgct gaagttaggg cacagattga
     7081 tgcagccgcc gagtgcggca tatacgagtt catgctctgg aacccgagga acctgtatac
     7141 agccggtgcg cttgggcgtc cggatccgga ggatgcgctc cgtcctaggc cgcatttgga
     7201 cctgcttgcc ggaagcggtg ctatccgtct tgaggatccc gtgacagtca ctgaatggcg
     7261 ggcattgtcg gcactgaaac aggccggcgt cggccagatc ctgggccctt aggccaggcc
     7321 ggctgggttg cgatggggcg gccttggttg cacgagtcca ggcgcaggtc tataattgga
     7381 ttaaattcgt attagctgta gatgcgggtg gaaataagta attgagggtt ttgaggtgag
     7441 ttacgtgctt cgcactcata cttgtggcga gcttgcagca ggaaatgttg gtgagactgt
     7501 tgcgctgtgc ggatgggttc agtcagtgcg cgaccacgga ggattgatct tcgttgatct
     7561 tcgggacaga tacggtcgta cgcaggtagt tgtcagtccc gatctttcag aagatatttt
     7621 ggagcgcgca aggagcattc accgcgaaag cgcgatccgg gtggagggaa ttgtctcaga
     7681 aaggccggaa ggcacaaaga atccttcgat atctaccggt gatgtcgagg ttatggccag
     7741 taatatagag attatagggg atgctgcccc ggtgcttcct atggaagtgt cagatgagaa
     7801 cttggccaac gaagaggtca ggttgagata cagatacatt gacctgaggc gtccctcgct
     7861 gcaggcaaac atgatggcgc gtcaccgcgc tgccatggct gtccggaact atctttcgga
     7921 ccagggcttt cttgaaattc agacaccgct gttggtgcgg agcactccgg agggagcgcg
     7981 cgactttgta gtgccaagca ggaatttccc tggtaagttc tatgcacttc cgcagagccc
     8041 tcagttgtat aagcaattgc tcatgatagc gggatttgat cgctatttcc agatcgctcc
     8101 gtgtttccgc gatgaagacc agcgggctga caggcagtta gtgcacagtc agatcgatat
     8161 agagatgagt ttttgcgatg aagaagacgt gttttcggta gttgagggca tcatatccgc
     8221 agcattcaaa ggggcgatcg gggcagacgt caaaggacca ttcctcagga tgacgtatga
     8281 cgaggccatg gagaggttcg gatctgacaa accggacatg aggttcggga tggagctcgt
     8341 agatctcacg gatctcgtgg cggattcgga attcggcata ttcaagagtg tgtgtgccac
     8401 agggggtcgc gtccgaggaa tctctgcccc tggttgtgca ggcttttcgc gcaagcagat
     8461 agacgacctt actgaactgg ctcggatcta tcgtgcgaag gggctgatta ccctcaaagt
     8521 cgaagggggc gagatccaag gaagcgttgc caagcacata tcctctgaca ccctcggtga
     8581 gatcgcatcg aggctgggca gcgccgatgg agacctcata ctgatcatag ccgatacccc
     8641 tgaggtgtct gccacggctc tggggcattt gcggcgatat ctcggtaagg agctcggcct
     8701 tatcccgaag ggtgattaca aactcctttg ggttacagat tttccgctat ttgaatggaa
     8761 tccagatgat ggccgatggg atccgatgca tcatatattc accatgccgc gggagggaga
     8821 cctcgagttg ctcgagtcag atcccgcctc tgtcaagggt cgccagtatg atctggttct
     8881 caatggcaca gagctcggga gtggaagcat caggattaac gatcccgcta tacagaagag
     8941 ggtcatgaac gtcatcgggg ttacccccaa ggaggcagag gagaagttcg gattcctgct
     9001 tcgcgcgtac caatacggcg gcccaatcca tggcggattc gccatcggtt tcgacaggct
     9061 cctagctgtt atgcttggac ttgaaagtct gcgcgatgtc atcgcttttc ctcagaatgc
     9121 cgcaggcgtt tctctggtgg acgactgccc gaacgagata gacccgaaac aatggaaaga
     9181 gcttcatctg aagcttgaag attgtgcggg caagcccgat gccgaggcgc gttcggactg
     9241 ctgatgcgtg cggcatgagg aggccgtttg gtccgtgcgt tccagggaac ggggcgcacg
     9301 ggcttttgat taatccattg atgtctgatt cagggttgcc ttgactaaat acttatgctc
     9361 cggaggtaga ttacgacatg cataaaccag acaagatcag ggaggctctc gcctttgacg
     9421 acgttttgct tgtcccgggc atctcagaag tccttccagc caatgtagat gttaggaccg
     9481 agattactga taagatcatg ctcaatattc cgctgatgtc ggctgccatg gatactgtga
     9541 cagaggctcg aatggccgtc gcccttgccc gtgaaggcgg aatgggcata attcacaaaa
     9601 acatgtccat tgaagagcag gccctcgagg ttgacaaggt gaaacgctca gaacacggca
     9661 tcattgtcga tccgatattc ctttcgcctg aagcctcagt aagagaggca cttgagatta
     9721 tggaacgtta tcatatctcg ggcgtcccaa taactgaaga cggtacactc gtcgggatac
     9781 ttaccaacag ggatctgaga tttgagaccg attacgatca gccggtgtcc aacgttatga
     9841 ccaaagatgg tttgatcact gcgcccgtgg ggactactgt cgatgaggct atcgagatac
     9901 tcaaggtgca caaagtcgag aagctgccac tggttgacga gaaattcaag ttgaaagggc
     9961 tcattaccat caaggacatc aagaagacgc gaaaataccc caacgcagcc aaagatgaac
    10021 acgggagact tcgcgtaggg gctgccgtgg gggtctctgt tggcaccatg gatagggttg
    10081 ctgctctcat cgatgctggc gcggacatga tagttgtcga tacagcccac ggacattcta
    10141 ggggcgtcat cgagacggtg aaggctatca gaaccaattt cggagatatc gacatcatgg
    10201 cagggaacgt tgctactggt gacggcgtca aagcattagc agaggcaggc gcgaactgtg
    10261 tcaaagtggg tatcgggccc ggctccatct gcactacacg tgtagtggca ggggtcggag
    10321 tcccgcaatt ctcggcgatt ctcgagtgtg ccgctgcagc cgcggaagct ggaatcagga
    10381 tagtcgctga cggtggtgtc aagttctccg gagacatgac taaagcactg gctgcgggag
    10441 cgcatgtggt catggttggg caactcctcg cagggaccga tgagagtcct ggcgatacag
    10501 agatctacaa ggggagaagc ttcaaggttt atcgaggaat gggttctata agcgcgatga
    10561 aggctggatc gagcgataga tacttccagg agggtgcaaa gaagcttgtc ccggaaggaa
    10621 tagaggggcg ggtgccctac aagggatctg tttctgacac tgtttttcag ctagtgggcg
    10681 gtgtgaaggc aggaatggga tatgtggggg ctgcgaatct tgaggagctc agggagaagg
    10741 ccacgtttgt gcggataacc aacgcaggcc ttagggaaag ccacccacat gacgtgcata
    10801 ttacgaaaga ggccccaaat tacatgattg ccggggccga gtacgaagaa tcgtaggcta
    10861 ctttttccga gaggaggacg acttgcggtg tcgaccaaga tcaagaggtg cagtcttttg
    10921 gctgtaagcc taattatggt tatggttgtg tccatgatct cgagcgagtc tgctctggcg
    10981 gagtatgatg tgaagagcat aatcatcaac ccgaatcccc cctatcagac cagcatttgg
    11041 actgacaagt cgcagtataa tccgggggaa aggctgaatg tctacttcag gacgagcaaa
    11101 gatgcgtata tctacatttt tgacatcgat accgtcggca acgtgagcct gatattcccc
    11161 aacatatact cagaatacaa ctacaaacgg gcaggtcaga catactcctt gcctgacaac
    11221 tccagatata acctgaccat tggcggtcct gcaggagttg agcagctcgt actgatcgcc
    11281 acgccatcgc agatacgcga cgttgaatgg ctaaggcgtt cacttgagca gggttcattc
    11341 gggccacagc taaatgtcaa tatatccgca gacaggttca tgtttgaggt gaagtcgatt
    11401 tcgattaccc ccaacttcgg ccaagattgg tcgtcggcgg tcgcaacatt tacagtgggg
    11461 agtgttgtaa cgccgccgcc gccaccaccg tcggtgaaat acggaacgct gagcattacc
    11521 tctaagccgt cgggggcaac cgtttttctg gacggggtag accaggggac cacgccgctg
    11581 acgattacgg gcgtgagcta tggcgtgcat gacattgcga taatgaagaa gaattactat
    11641 acgtattcgc gccagatcac ggtgaactcg ccaaatccat accgaataga tgcgacactt
    11701 gtgaagctgc ctacgtcatc cgattggggc gacgaggttc agcttgtaaa caagagcatg
    11761 ctggtaaaat ggcctcaaac cgggccgttc actgagacgt tcacttttag aggttctacg
    11821 ggcaaggtgg tcatcaacgc agacccgatc ctcggcatgc tgtcgagagt gacaggaagc
    11881 atcactgcga atgcagtggg gccagtggaa ttcatgcaga tcaacccgag tgggcctgat
    11941 gcgccctggc ccggaagggt atatgaaagg attcagtatc cgttcagggt gagggtaact
    12001 gtagaggatt acagcatcac gacgggtctg gtctttggaa ttgaatacat cgactacgta
    12061 cggttgcgtt tggagatttg gcatataggc aactgattct ttagcacgag acggttgccc
    12121 aaaaatatag cagcacggca gagcagggtc ggggtttaga ctccccgacc ctgctcatat
    12181 ccgaaactcc atgtggatgt ttggatgtgg tgccacattt cgtgtggcag ggccgagtcg
    12241 atcagtgcat ctgaggtagg gacgtgttga agccagtagg atccatgatc cagcagtgtc
    12301 tcgccgcatg caccatgttg ccgccctagc tctgttcaat gcgccgcccg gcccggcacg
    12361 caacatgtaa aagactacta taagctagca agcttgatgg gtgcacgcgg cagtaccatg
    12421 ccagacgacg gcctcgggtc ctttaacata tgcttattgc agagggatga aagacattgc
    12481 gccggtaggc ccaagaaaaa cggtcatgtt ctttagcaga ggcccgcgtc tgttttggga
    12541 tcggggtagg gaggagagag ttctatgctt caccgcagac cgcgtattag ctggggcaag
    12601 gctgatactc tatagtggaa atggtcaggc gcttcacggt cacatattcg cggctgctca
    12661 gtcctttgcc gcatacgcct acctggacgt cgcgtacaga tgtgacccgt ccggcgggga
    12721 ggacagatcc gatccgtaat tgggctgtgg ttgagaaacg ttggctgggc aattataata
    12781 gtagttacta tggaaacatc ttgatgaaga tgaggtcatc cggaggtgtg gttattgcag
    12841 tccgtccgta tcgccgacat acgagaacat gatggagaag aggtcactat taaggggtgg
    12901 ctatataaca agcggtcgag tggaggcatc catttcctga taataaggga tgggacaggt
    12961 ttcatgcaag ctgtcgccgt tcgtggcgaa atccctgatg accagtttga actgtgcaac
    13021 aacctgacgc aagaatcgtc gatattggtc accgggcgca ttcgcgctga caagagggcg
    13081 gcgggcggat tcgagatgtc agctacaggc gtggagcttg tacagctggc tgaggaatac
    13141 ccaataaccc taaaggaaca cggcgtagat ttccttcttg atcacaggca cttgtggatt
    13201 cgtaatcctc gacaacacgc tgcgttgctg gtgcgggacg aggtgatttc tgcggcggaa
    13261 gactggctgc gaagcaacgg gttcgtcaag atcgacgcgc caattctgac gccggcggca
    13321 tgcgaaggaa ctactacctt attcgagacc gactacttcg gcgaaaaggc gtatctgagc
    13381 cagagcggcc agctgtacaa tgaggcggcg tgcatggcat ttggcaaagt ttactgctac
    13441 ggtccgacct ttagggctga gaaatctaaa acgcgaaggc atcttatcga gttttggatg
    13501 atcgagcccg agatggccta tgccactcag gaagacaata tggtgattca ggaagagttc
    13561 ttgtcataca tcgtacagcg ttgccttgcc aaacgccggc aggagctgat cacgctcgaa
    13621 agagaccttt cgaaactgga gtgtattgtg ccgccattcc cgcggctttc gtatgatgag
    13681 gccgtggaac tgttgcacgg ggagggtaag gaattcgagt ggggagacga tttcggggcg
    13741 cccgacgagg ctacgatttc gatgaaatac gacaagccgg tatttgtaac cggttatcca
    13801 acaaaggtaa aggccttcta tatgcagcct gatccggata ggcccgagat agtaaaatgc
    13861 tctgacttgc tggcgcctga gggctacggc gagatcatcg gcagcagtga gagaattcac
    13921 gacctggatc tcctggcggc gcggatgcgc gagcataatc tccctgagga agcctacggt
    13981 tggtaccttg atctccggcg ctatggatcg gtcccccatt ccgggttcgg gctagggatc
    14041 gaaagaacag tagcgtggat taccggcagc gagcatgtta gggagatgat tcctttcccg
    14101 cgcctaatca atcgcatata cccgtagttc atgaaatatc tattggagcg ctcgatggtg
    14161 gaccccgcgt gacgaacagg caagcggggt tcttgcatat tggaaccgct ggcttcgcac
    14221 agcgtctata cctactgagt ccaacaaata gggagggcag aggtaatgaa gagcaaaagg
    14281 aaggtgctat tcatcttcgc tagcttagcc ctggtgcttt tcgtaggcac attggtgtca
    14341 actagttcgc aagcgcagat ttcagcttat gattacattc tgcggggcga gcagtatctc
    14401 gcacagggtg ctgcaaatga agctataggc cagtttgaac aggcaatata catagaaccg
    14461 acaaacaccc gcgctcactt cgggcttggg tgcgcctacg agatgaggtc gaaggctgcc
    14521 agggtgttca attatcggat ggagcttttt acggaaccgg attcagtcag aaagctgggc
    14581 ttcccacccc tttcagaaca gggatatcaa gatgtcgaac gagcgattca agcctactca
    14641 aaggcggcac tctactcgcc gccgtatgca gaaggccatt accgacaggg agttctccat
    14701 ttcattagcg gaagacccgg agatgccaaa gttgccttgg agagggccgt cgctcaggat
    14761 aggacggcag tcgaccctgc tgttctcctt gcctatatat acctggagga agggaatatc
    14821 caggcagcga ctgcgcagat tgatcgtatt atctctgcaa ctggcgacag gctcaacccc
    14881 gtgattcttg gtgtatctgc agtgttggaa tacaggagtg gcagatatga tcaggcagag
    14941 cagttgctgt tgcaggctac aagcaggtct ggcgcgccag cctatcttta caagcggctt
    15001 ggcgatgcga tggctgcgcg cggactccac acgcaagcat tggatgcata ccgtcaggcg
    15061 gtgcagaggg atcccgagtc gatactggct aatgatgcaa ttggcaacat atacaggctt
    15121 tcagggcagt cggcacaggc tgtgcagtgc tattcagctg ccgcgtcggc caatccgaat
    15181 ctgttacggg caagatacgg acttggttcg gctctgctcg aatcgggttc atatgcccaa
    15241 gcattgcaga cgttcagcga acttgtggac gcattgccaa cgtatggatc tgataatgcg
    15301 ctgcttttaa agggtatagc ccagtggaag accggcaatg caaatgcggc gcttgccact
    15361 gtcaaccatg ccctgggaat caatgctgaa aaccgcatag ccgccattta caaggagcgc
    15421 atcgaacgtg aaatgggcgg ttacggtgta tcgccccttg gcgtgcagcc caagtccata
    15481 gtggcggaag ggagcaaacc ggacaaaccg ttcagcggaa tcgtgataaa cgatggcgct
    15541 gaatatacaa acagccggac ggttcggctc gcagtttaca gtgattactc aactgtcgct
    15601 tttagtaatg acaattccac ctggagctcc tggttttcga agcctgagcc atatggagag
    15661 ttcacttggg aattgactcc gggagacggg acaaagagag tatacgtgaa atttcgtggc
    15721 ctattcggta taggcatacc cggcgaatcg gggacgatag tgctcgactc gacgccacca
    15781 gtagccactg cacgcttgga gagcatgcaa acaggcggtt acggaggtta tgatggcagg
    15841 acgagagttg tcctttcggc caatgatcct agtgggatag tcgggttttg gatgtcctat
    15901 gacggcaatg attggcgctg gttcgactgg tatggaagtg attttgccat atctttgcct
    15961 gcaggtgtcg gtgcgacgcc gagggtccgg ttttccgtgg ttgacggagc aggcaaccat
    16021 gcgtcg
//
LOCUS       DAOSYL010000052        15720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3125_length_15720_cov_8.663645, whole genome shotgun sequence.
ACCESSION   DAOSYL010000052 DAOSYL010000000
VERSION     DAOSYL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15720
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_15720_cov_8.663645"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..1904)
                     /locus_tag="PLU15_08345"
     CDS             complement(447..1904)
                     /locus_tag="PLU15_08345"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOO30969.1"
                     /translation="MSKEWTGPLERIEECMWMIPKTYKPGMLADAVVIADEESLRTAC
                     EGEALEQLANSAFLPGICSPAMAMPDIHSGYGFPIGGVGATDLESGVITPGGIGFDIN
                     CGVRLVRTDLEAGDIAPKIKPVIDRLYRDIPSGLGTSGKISLSAQEVRKVLQEGAEWA
                     VKAGYGFEADLEHTEEGGSMEGADASAVSKKAVERGMPQLGTMGSGNHFLELQVVEEI
                     VNPEVAKVFGLFEGQVVMMIHSGSRGLGHQVCTDYISTMMSALKKYDISVPDRQLACA
                     PLGSDEGKQYFAAMAAAANFAWANRQCIMHWAREAVAAEFGSTPEKLGMTLVYDVCHN
                     IAKVEEHIIDGSKKRVCVHRKGATRAFPPNHPDIPKAYRGVGQPVIVPGDMGRNSWVL
                     VGTRQAMEKSFGSTCHGAGRMMSRKEALRTRKPQKVRDALAEKGIYARSASRGALAEE
                     APWAYKNVNDVVDICHRAGISSKVARLRPLGVVKG"
     gene            complement(2023..2625)
                     /locus_tag="PLU15_08350"
     CDS             complement(2023..2625)
                     /locus_tag="PLU15_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOO30970.1"
                     /translation="MATERIFIEPFGEAGDAAYRVYASTRELLFAGCGLALSNLILPS
                     AWEEAEILEPIAERSFAAGGTLMETRSAVSSASILNVVPGASPAERSFVLSSRDLDLL
                     LVDFLNELIYLFDSEGFWGYSFEPKFADPKSKDPAGSVDAEMRLAVRTSGVILPPSAL
                     EPEQPEDGILGIVPKGVSYHMLEAGQGKDGRWHAQFVIDA"
     gene            complement(2650..5094)
                     /gene="gyrA"
                     /locus_tag="PLU15_08355"
     CDS             complement(2650..5094)
                     /gene="gyrA"
                     /locus_tag="PLU15_08355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO30971.1"
                     /translation="MTELVDGKIVPIDIENEMRKSYLLYSMSVIVGRALPDVRDGLKP
                     IHRRILYAMHGLGLTADKPYRKSATVVGEVMGNYHPHGDAAIYDALVRMAQDFSMRNT
                     LVDGHGNFGSVDGDPPAAMRYTETRMTRLAAHMLADIEKDTVNFVPNFDGSKKEPEVL
                     PARFPQLLVNGSSGIAVGMATNIPPHNLVETIDGTIELIDNPDATIEDLMEHIKGPDF
                     PTGGLILGTQGIREAYHTGRGSIKVRARARIEPMSGGRHRIVVTEIPYQVNKARMIER
                     IAQLARDKRIEGITDLRDESDREGLRIVMELKRDANPNVVLNQLYKHTPMETTFGAIM
                     LVLVDNEPRVLNLKEMLHYYIEHQKEVVTRRTQYDLSKAEERAHILEGLRIALDHIDE
                     IISLIRASRTREIAREGLITKFGLSDRQAQAILEMRLQSLTGLEREKIEQEYEEVCAL
                     IAHLREILGNEQLLLGVIKDELIEIRDKFGDPRRSEITQDFSELEIEDLIAAEDVVVT
                     MSNQGYIKRLPINAYKAQRRGGKGVTGMATREEDFVEHLFITNTHDTILFFTNMGKVY
                     SLKGYEIPEAQRHARGTAVVNLIELDPGEAVTALMPVSKFDEDVYVFMATKNGTVKKT
                     ELSAFANIRRSGIIGISLAPDDELIAVRMTGGDDEILLVTKDGMSIRFRQDNVRAMGR
                     TAAGVRGINLDKGDAVVGMDIARDNADVLIITSAGYGKRTPVVEYRPQNRAGKGLKAC
                     RLTNKTGNIVGVKLVREENEMMLTTMAGYVIRFQISDVPRMGRLTQGVKVMSLDDGDQ
                     VISIARVSSKGEDDLE"
     gene            complement(5106..7034)
                     /gene="gyrB"
                     /locus_tag="PLU15_08360"
     CDS             complement(5106..7034)
                     /gene="gyrB"
                     /locus_tag="PLU15_08360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOO30972.1"
                     /translation="MGHEAQNDNNNLAYNAAQIQVLEGLEGVRKRPAMYIGSTDSKGL
                     HHLVYEVVDNSIDEALAGYCDTISVTLGKDGSVTVEDNGRGIPVGIHPHEGKPALEVV
                     LTKLHAGGKFGGGGYRVSGGLHGVGLSVVNALSERLEVWVKLDGRLHYQGYTRGVPDA
                     PLRVIGEASGTGTTIKFVPDATIFETVTYSYESLASRLREFAFLSAGVKLILRDERGD
                     EAREAVFEYEGGIRSFVEYINRAKDPIHPDVVYVGKVLDKVTVEVAIQYNDGYNENML
                     CFANYVNTTEGGTHLSGFRSALTRSINDYARKSGLLKENDDNLTGDDTREGLTAVLSV
                     KLEDPQFEGQTKTKLGNSEIRGIVDSVLSEGLSTYFEENPATARAIVEKCVSAARARE
                     AARKARELTRRKSALEISSLPGKLADCTLRDPELCEIYIVEGDSAGGSAKSGRDRRFQ
                     AILPIRGKIINVEKARLDRILANNEVRAMVTAFGTGIGEDFDLSKLRYHKIVIMTDAD
                     VDGAHIRTLLLTFFFRFMTPLIENGHVYIAQPPLYMVKEGKKEHYVYSDRELDELMNA
                     LGKGNYPIQRYKGLGEMNPEQLWETTMNPQTRTMLRVTMEDAIAADQIFTTLMGDKVE
                     PRREFIETHAKFVTEIDI"
     gene            complement(7133..7429)
                     /locus_tag="PLU15_08365"
     CDS             complement(7133..7429)
                     /locus_tag="PLU15_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOO30973.1"
                     /translation="MVYQSEKSGLHGRYMYIGSDVLIDGRDVVAIFDMKSVSRSREMK
                     AFMESKKKAGKVIDISEGEPKAIVVTSEDVYLCPVSVGTLRNRISGPFDGEGDE"
     gene            complement(7429..8388)
                     /locus_tag="PLU15_08370"
     CDS             complement(7429..8388)
                     /locus_tag="PLU15_08370"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOO30974.1"
                     /translation="MMLPIGDVLGKSMDKMGYTARIRATSAMLVWNDEIAPSLAVASR
                     ATFVRGKTLYVTVEKPVWAQQLSMMKRGLVQRINASAGKDAIRDIRFRSGQVRAPLVW
                     GADSEAGLKFPSPSAQEEGVPDWKSMPADEDALSTAANASRPIGDPEVREKFSDLMIT
                     DAKWRAWSRQNLSSNAVKAADILKKEPWLSDSHLASMVPGASSDDLIRARDAAAQELR
                     REIASLVAVDRLESRFASGISEAPTGKSSCLHEDATDRGVDSLGRQGIVGDAKRLVRI
                     RLLVESLAMLVAGEPPDKIDEELVERVLGSEYADLVRYLSEEL"
     gene            complement(8288..9529)
                     /gene="recF"
                     /locus_tag="PLU15_08375"
     CDS             complement(8288..9529)
                     /gene="recF"
                     /locus_tag="PLU15_08375"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOO30975.1"
                     /translation="MRNIQLSGFRNYSKESVTFDPGLNLFLGHNAQGKTNLLEAVYVA
                     SAGRSYRASSDSELVQKGSSYYRVAVDVQRRPAPVTLEVSYSTDGRKLVRLNGTERIR
                     AAELSSFLNVVLFTPDDLALVKGSPSVRRKFLDLEISQVSPAYRQLINLYSKIVTQRN
                     ALLKQARERNWRSDSAAASRLLESWDEQLVETGSKITARRMQVVASLDELGREAHYRI
                     TGVPGALSVRYLPSVPLSADEQGSTSEATVDWIAERYRARLRQLRKVELIRGVTMVGP
                     HRDDISFQLDGADVATYGSQGQQRSTVLAVKLAEANFIRAHTGDMPVLLLDDVLSELD
                     ETRRTNLLGEIVGDNQVLVTSVEPGCRDLMPRGAAVFSIVAGEVTPDDASHRGCSGKE
                     HGQDGIHRPDPGDIRHASVER"
     gene            complement(9541..9789)
                     /locus_tag="PLU15_08380"
     CDS             complement(9541..9789)
                     /locus_tag="PLU15_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOO30976.1"
                     /translation="MKNMTLEIETEYIELDQALKLAALVGSGGEAKMAIHSGWAKVNG
                     AAEFRRGRKLRDGDVLEFADVVVTIVSGGSSGSGKEDV"
     gene            complement(9803..10939)
                     /gene="dnaN"
                     /locus_tag="PLU15_08385"
     CDS             complement(9803..10939)
                     /gene="dnaN"
                     /locus_tag="PLU15_08385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOO30977.1"
                     /translation="MKVSCSKAALVSGVQTVYKAVSPKATIQALSGVLVETCTQGLRL
                     VAYDLDELGIETFVPVTVGTEGSTVIPAKYFSDISRKLPQSEVILEYREESQSVSISA
                     GRADFTVKTMPAAEFPALPEISEANSWSMPESELKTMIRRTIPAAAVDDARAFFKGIY
                     MLFEKDSLTMVATDSFRLAYRKTQASFDLEEPIDVIIPAKALLEVERNLNDTGEREVS
                     IAIEKNHAMFRIGPTTYVTRLLEGQFPNYKQVFPENIPAKIICDRLQLLDSVDRVSLM
                     CKDELSAIRMSYTGDEGMASGFLTITAATPEVGEGKEDIPVSMIGHAEVTVAMRARYL
                     RDVLRVLDGEEVCLGYTSYRHPIEVTDETDPAYTYLLMPVTAPV"
     gene            complement(11228..12685)
                     /gene="dnaA"
                     /locus_tag="PLU15_08390"
     CDS             complement(11228..12685)
                     /gene="dnaA"
                     /locus_tag="PLU15_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOO30978.1"
                     /translation="MDVLQVLESELSRPSFDAWLKTSVPVVVADDIVEVPVPHDFARD
                     WLEMRYAALLSETMSSMVGRKITVRFIARESSAEAENGDGAVSEDSYEMGNHANPATR
                     EPSPANALDANAASGMGVDEAAATSGTYAQTQAEAKAFDDFSTPALNPKYTFESFVVG
                     NSNRFAHAAALAVSEAPGKAYNPLFIYGGVGLGKTHLMQAIGHHVLEINPYAKVVYVP
                     CEQFTNELINSIRFETTIQFRERYRNVDVLMIDDIQFLAGKERTQEEFFHTFNTLYES
                     GKQIVLSSDRPPKEIATLEARLRSRFEWGLITDIGTPDLETRIAILKKKAVSEGLDVP
                     NDVLVYIAGQIHSNIRELEGALIRVTACAALTNREITTQLAAEVIQDMIPSSRPKQIS
                     VELIQRTVADYYHLEVSEMVSKTRVRTVALPRQIAMYLCRELTELSLPKIGEEFGGRD
                     HTTVIHACDKIQNELKKDSTMNANIKEIVSRLKSS"
     gene            complement(12736..13977)
                     /locus_tag="PLU15_08395"
     CDS             complement(12736..13977)
                     /locus_tag="PLU15_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO30979.1"
                     /translation="MALFKFLFGRMNGEKSQTEDSVAEVSAIAPESAPAYTLRIATYD
                     SMSILPRTIDLSSDSFASFVDEASSRVYAFCKEKGGSMPFVVLREAIENLIHAGFEDA
                     VITIMPDGNVVRISDHGPGIRAKSQAMMPGFTSATADDRKLIKGVGSGFPIMIESLES
                     LGGFLTVEDNLGSGLVVTLCSSPDARPGGVSPKPDRASQKTAGPSQPVRHDPAHMQPA
                     QIAQGFTEAAWSSRQDISGAYHPPTQGIFIGHPQDGDGHIFAEGPQGSGGVRPQRDPG
                     AEISQTSSAVYNESDSIDMENIADEGRATLSVEKQDSAADQSKKRRDEEPPSTSQSSD
                     MSDEALGALLSSRQKKVFLLIAEMGEIGPSVVTKELDISLSTAYRDLVTLEELGLVVS
                     LEGGKRKLTRRGVDFLSYVFR"
     gene            15434..>15720
                     /locus_tag="PLU15_08400"
     CDS             15434..>15720
                     /locus_tag="PLU15_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30980.1"
                     /translation="MASHLGFEGRLTIRFPHGKSPFCSTKNRATGPKCKMLQIVQTTW
                     IEPQKQLFRWNEAVEKLGDLGQLKLVIDYLPDEELMQALEKVRGNGRHHLL"
BASE COUNT         3473 a         4473 c         3885 g         3889 t
ORIGIN      
        1 ttatcaagct tcacgctgtt tggttttcaa ggaacatccg cggctttagc gccgcgccgc
       61 atttgagctt accgccctgc ggcaaaagat atattatcac atgagttgag atgttgtcaa
      121 atccaatttt cgtctctttg tttgtctacc gatccttgcc cttaccaatc aagagaaatg
      181 actgatcggg ctattcgacc tgtttaccac ttttcttttc atgaaaagta gtttctatcc
      241 cgctgcttgc ttttcataga atcaaactat cagagtgaaa aatgaaaagc gcctatgcag
      301 cgaggccgat tggaaagctg cacgcgcatc accttgtgca agtgcagctt tagtatctta
      361 caagggccgc cttgaaaacg caacaggaat tatatggtcc acaaggacgt cctcacctaa
      421 accggcgcca gccgcgccga tctgttctat ccctttacga ctcccagcgg cctaaggcgc
      481 gcgactttgc tcgatatccc tgccctgtgg cagatgtcga ctacatcgtt gacattcttg
      541 taggcccaag gcgcctcttc ggctagcgcg cccctgcttg ccgagcgcgc gtaaatgccc
      601 ttttcagcga gggcatcgcg gaccttttgg ggttttcgag tgcgcagtgc ttcttttcgg
      661 ctcatcatcc gccccgcccc atggcatgtt gaaccgaagg acttctccat tgcctgcctt
      721 gtccccacaa gcacccacga gtttcgcccc atgtcacctg ggactatgac cggctgtccc
      781 actcccctgt aggcctttgg gatgtctggg tggtttggcg ggaaggccct tgtggcgccc
      841 ttgcgatgga cacagacccg cttctttgag ccatctatga tgtgctcttc gaccttagcg
      901 atgttatggc acacatcgta gaccaaagtc atgcccagtt tttcaggcgt agagccgaac
      961 tccgcggcaa cagcttccct ggcccagtgc atgatgcatt gcctgttggc ccatgcgaaa
     1021 ttcgcggccg ccgccatcgc ggcaaaatac tgcttacctt cgtctgaacc gagcggagcg
     1081 catgccagct gcctatcggg cacagagatg tcatacttct tcagagcaga catcatagtg
     1141 gagatgtagt cagtgcagac ctggtggccc aacccccgcg agccggagtg tatcatcatc
     1201 accacttgcc cctcaaaaag gccgaaaacc ttggccacct caggattgac gatctcctca
     1261 acaacctgta gttccagaaa atggttgcca gatcccatcg ttccgagctg aggcattcca
     1321 cgctctacag ccttcttgct tactgcggaa gcgtcagccc cctccatgct gccgccctcc
     1381 tcagtatgct caaggtcggc ctcaaaacca taccctgctt taacggccca ctctgctcct
     1441 tcctggagaa ccttgcgcac ctcttgggcg ctgagactga tcttgcccga agtgcccagg
     1501 cccgacggga tatcgcggta gagtctgtct attacaggtt tgatcttggg ggcaatgtcc
     1561 ccggcctcca gatcagtcct gacgagcctc actccgcaat tgatgtcaaa acctattccc
     1621 ccgggggtga taacgccaga ctcgagatcc gtcgccccaa cgccgccgat tggaaagccg
     1681 tatcccgagt gaatatccgg cattgccatg gctggcgagc agatccctgg aaggaatgcg
     1741 gagttggcca gttgttccaa ggcttcgcct tcacacgcag tcctcaaact ttcctcgtcc
     1801 gcaatcacta ctgcatccgc cagcatgccg ggcttgtaag tctttggtat catccacatg
     1861 cattcctcta tcctctccaa cggccctgtc cattccttcg acacggcgtt cacctccttg
     1921 aaaatgctaa atgccgataa aaccggaaca ctaagcaagt ctcgataagc ttctgggcaa
     1981 tcctcgagat cacattacct cgatagtgcc aagtaagccg aactacgcat cgatcacgaa
     2041 ctgagcatgc caacggccat ccttgccctg tcccgcctct agcatgtgat aggatactcc
     2101 tttcgggaca attccaagaa tgccgtcctc cggctgctca ggttcaagag ccgagggagg
     2161 caaaatcaca ccgcttgtgc gcactgccaa gcgcatctcg gcatcgactg atcctgccgg
     2221 gtcctttgat ttcggatccg cgaatttcgg ctcgaagctg tatccccaaa agccctctga
     2281 atcgaagagg tatatgagtt cgttcaagaa atccaccagc aaaaggtcga ggtctcgtga
     2341 actcaatacg aatgaccgtt ctgcagggga agcgccggga accacattca agatgctagc
     2401 ggacgagact gcgctacgag tttccatcaa agtgccgccg gcggcgaagc ttcgttcagc
     2461 aattggctcc aagatttcgg cttcttccca tgcagaaggc agtatcagat ttgaaagggc
     2521 aagcccgcat ccggcaaaaa ggagctcacg cgtgcttgca tatacccggt aggcggcatc
     2581 gcccgcctca ccgaatggct cgatgaagat gcgctctgtc gccatgtgaa atcacaccac
     2641 tattcagtcc tattccaggt cgtcctcacc cttggaagag acccgcgcga tcgagatgac
     2701 ctggtcgcca tcgtcaagac tcatcacctt gacgccctga gtgagccttc ccatcctggg
     2761 cacatccgat atctggaacc ggatgacgta gcccgccatg gtagttagca tcatctcatt
     2821 ttcctctcgc accagcttga cgccgactat gttgcccgtc ttgtttgtga ggcggcaagc
     2881 cttgagaccc tttcccgcgc gattctgggg acggtactca accacaggag tccgctttcc
     2941 atagcccgca ctggttatta tcaagacgtc tgcgttgtcg cgggcaatgt ccatgcccac
     3001 aaccgcgtcc cctttatcaa gattgatccc gcgaactcct gctgccgttc ggcccatcgc
     3061 cctcacattg tcctggcgga acctgatgga catgccgtcc ttggtaacaa ggagtatctc
     3121 atcatccccg ccagtcatcc tcacagcgat aagctcgtca tcaggtgcaa gcgagatgcc
     3181 gattatgccg gacctccgta tatttgcaaa ggcgctgagc tcggtcttct ttacagtacc
     3241 gttttttgtg gccatgaaaa cgtagacgtc ttcgtcgaac ttggacaccg gcatcaatgc
     3301 tgtaacggct tctccggggt caagctctat caaattgacc acagcggtgc cccgcgcatg
     3361 gcgctgagcc tcgggtatct catacccttt caggctgtaa accttgccca tgttcgtgaa
     3421 aaacaagatt gtgtcatggg tgttcgtgat aaagaggtgc tccacgaaat cctcctcgcg
     3481 cgtggccatc ccggtgacgc ccttgccccc acggcgctgc gccttgtacg cattgatggg
     3541 caggcgcttg atgtagccct ggttgctcat tgtcactaca acatcctcgg ccgctatgag
     3601 atcttcgatc tccagctccg agaagtcttg ggtgatctca gagcggcgcg gatcgccaaa
     3661 cttatcgcgg atctcgatca gctcatcctt gatcactccg aggagaagct gctcgtttcc
     3721 taggatctcg cgcaaatggg ctatgagcgc gcacacttcc tcgtattcct gctctatctt
     3781 ctcacgctcc aagccggtta ggctctggag gcgcatctcc aagatagcct gggcctgtct
     3841 gtcagaaagc ccgaatttcg taatcaagcc ctctctggct atttctctcg tccgggaagc
     3901 ccgaatcaac gaaattatct catcgatgtg gtccagcgca atcctcaagc cttcgagtat
     3961 gtgcgccctt tcttcagcct ttgaaagatc atactgggtc cttcttgtta ctacttcctt
     4021 ctgatgctca atgtagtagt gcagcatctc cttgagattg aggacccgcg gttcgttgtc
     4081 gacaagcacc agcatgatgg cgccgaaagt cgtctccatg ggggtatgct tgtaaagctg
     4141 gtttaagacc acgttcgggt tggcatcccg cttcagttcc atcactatgc ggagcccttc
     4201 cctgtccgat tcgtcgcgca ggtctgtgat gccctctatg cgcttgtcgc gtgcaagctg
     4261 ggcaatgcgc tcgatcatcc tagccttgtt aacctggtag ggaatctcag ttacgacaat
     4321 cctgtgcctg ccgccgctca taggctcgat tcttgcccga gccctcacct ttatgcttcc
     4381 tcggcccgtg tggtaagcct cgcggattcc ctgggtcccg aggataagcc ctcctgtggg
     4441 aaaatcagga ccctttatgt gttccatgag gtcctctatt gtggcatcgg ggttgtcgat
     4501 caattcgatc gtcccatcta tcgtctcaac caggttgtgc gggggaatat tcgtagccat
     4561 ccccacggca atgcctgatg agccgttcac caagagctgc gggaaccgtg caggaagaac
     4621 ctccggttcc tttttcgatc cgtcgaagtt gggaacgaag ttcaccgtgt ctttctctat
     4681 gtctgcgagc atgtgcgccg ccagacgtgt catcctcgtc tcggtgtacc tcatggccgc
     4741 cgggggatcc ccgtcaacgg agccgaagtt gccatggccg tcgaccagtg tgttcctcat
     4801 tgagaaatcc tgggccatcc tcacgagggc atcgtagatg gctgcgtcgc cgtggggatg
     4861 ataattgccc atgacttcgc cgaccacagt agctgatttc ctgtaaggct tgtcagctgt
     4921 gaggccaaga ccgtgcatgg catagagtat cctgcgatgg atcggcttca gcccgtcgcg
     4981 cacgtcggga agcgctcgtc caactatgac gctcattgag tagagcaagt aggatttgcg
     5041 catctcattt tctatgtcga tcgggactat ttttccgtca actagttctg tcaagcccca
     5101 acacctcaaa tgtcaatctc tgtaacgaac ttggcgtgag tctctatgaa ttcgcgcctc
     5161 ggttccacct tgtcgcccat gagagtagtg aatatctgat cggccgcgat ggcgtcctcc
     5221 atggtcaccc ggagcatcgt tctggtctgc gggttcatgg tggtctccca caattgctct
     5281 gggttcatct ctccaaggcc cttatacctc tggatcgggt agttgccttt gcccagagca
     5341 ttcatgagct catcgagttc gcggtcgctg tagacataat gctctttctt gccttctttt
     5401 accatataga gtggaggctg ggcgatgtat acatgcccat tctcgatcaa gggcgtcatg
     5461 aaccggaaaa agaacgtcag caaaagcgtc ctgatgtgag ccccatccac gtccgcgtct
     5521 gtcattatca cgatcttgtg gtaacggagt ttcgacagat cgaaatcttc gcctattccc
     5581 gtcccgaacg ccgtcaccat tgcccttacc tcgttgttgg caagtatcct gtcgagtcgg
     5641 gctttctcca cgtttatgat cttgcccctg atgggcaata ttgcttggaa tctgcggtca
     5701 cgccctgatt ttgcggagcc gcctgccgag tctccctcga cgatgtagat ctcgcacagc
     5761 tccggatccc tcagcgtgca atctgctagc ttgccgggga gagacgagat ctccagcgcc
     5821 gatttgcgcc gcgtcagttc cctcgctttc cgcgccgctt cgcgcgcgcg ggctgccgaa
     5881 acacattttt ccactattgc gcgggcagtg gctgggttct cttcgaagta tgtcgagagc
     5941 ccctccgaca acaccgaatc gactataccc cggatctcgc tgtttccaag cttcgttttt
     6001 gtctgtccct caaactgggg atcctccagc ttcacgctga gaaccgcagt cagtccctct
     6061 cgcgtgtcat ctcctgtaag attgtcgtca ttctccttaa ggagtccgct cttgcgagcg
     6121 taatcattta tcgaccttgt cagcgccgat ctgaagccgg aaagatgagt gccgccctcc
     6181 gtcgtattga cgtaatttgc aaaacagagc atgttctcgt tgtagccgtc attgtattgt
     6241 atggcaactt ccacagtgac cttatcgagg actttgccga cgtatacgac atctggatgg
     6301 attggatctt tggccctgtt tatatactcc acaaaagaac ggattccacc ttcgtattcg
     6361 aagacagcct ccctggcctc gtcgccccgt tcatctctga gaatgagctt tacccctgca
     6421 ctgagaaagg cgaattccct caacctcgac gctaggcttt catagctgta cgttaccgtt
     6481 tcgaaaatcg tcgcatcggg gacgaacttg attgtcgttc ctgtgcctga agcctcgcct
     6541 atcacgcgaa ggggcgcatc aggcactccg cgcgtgtagc cctgatagtg tagacggccg
     6601 tcgagtttaa cccagacttc caaccgctcc gaaagggcgt tcacaactga caagcctacg
     6661 ccgtgaagac cgccggacac gcggtagccg ccgcccccga actttccgcc ggcgtgcaat
     6721 ttggtgagca cgacttcgag cgctggcttg ccttcatggg ggtgaatgcc gacagggatt
     6781 ccgcgcccgt tgtcctcaac cgttaccgaa ccgtccttgc caagggtgac agatatcgtg
     6841 tcacaatacc cagcaagagc ctcgtcgatg ctgttgtcga ccacctcata aacgagatgg
     6901 tggaggcctt tggaatcggt gctgccgatg tacatggcag gacgcttgcg aaccccttcc
     6961 aagccctcca agacctgaat ctgggctgca ttatatgcca agttattgtt atcgttttga
     7021 gcctcgtgtc ccaagaacat acctcccaaa agcaaaaaca aaccgaaaaa tggccgcggc
     7081 attgctaata cgcaccacgg cgcacaccaa gctaattata catcaaacag atttattcgt
     7141 cgccctcgcc atcaaacggg ccgctgattc tgttgcgcaa agtgcctacg gatacagggc
     7201 atagatagac atcttcagag gttaccacaa ttgccttagg ttcaccttca gagatgtcga
     7261 tcaccttccc tgcctttttc ttactttcca tgaaagcttt catctcgcgg gaccgactta
     7321 cgcttttcat gtcgaaaatg gccactacat cgcgtccatc gatcaatacg tcggacccga
     7381 tatacatgta tctgccgtgg aggcccgatt tctcactttg ataaaccatc agagctcctc
     7441 cgaaagataa cgcaccaaat ctgcgtattc agaccccaag actcgttcca ctaattcctc
     7501 atcgatcttg tcgggaggtt ccccggcaac aagcatcgca agagactcca cgagcaatct
     7561 tatcctcaca agtcgcttcg cgtccccgac gattccttgc cgtccaagag aatcaacccc
     7621 tctgtctgta gcgtcctcat gcaagcagga agatttgcca gtcggcgcct cgctgatgcc
     7681 tgaggcgaac cgagattcga gccgatcgac ggcgaccaac gatgcgatct ctcttctcag
     7741 ttcctgtgca gcagcatctc ttgcgcgaat caaatcatcg cttgatgccc ccggaaccat
     7801 cgaagctaga tgcgagtcgc tcagccatgg ctctttcttc aatatgtctg ccgccttgac
     7861 ggcattcgaa gacaggttct gcctgctcca cgcccgccat tttgcatcag tgatcatcag
     7921 atcactgaac ttctccctta cttccggatc cccaatcggc ctggaagcat ttgcagcggt
     7981 ggagagcgcg tcttcatcag caggcatact tttccagtca ggcacccctt cttcctgagc
     8041 agatggggaa gggaacttca ggccggcctc ggaatccgct ccccacacga gcggtgcgcg
     8101 aacttggccg ctacgaaacc ttatatcgcg gatggcatcc tttccggcgg aggcattaat
     8161 gcgctgcaca agccctcttt tcatcatgga aagctgttga gcccacacgg gcttttcaac
     8221 cgtcacatac agcgtcttgc ccctaacgaa agtggccctc gatgcaaccg ctagtgatgg
     8281 ggctatttca tcgttccaca ctagcatggc ggatgtcgcc cggatccggg cggtgtatcc
     8341 catcttgtcc atgctctttc ccagaacatc cccgatggga agcatcatcg ggcgtcacct
     8401 ccccggctac gatggagaat accgcggcac cgcgcggcat caaatcccta caccctggct
     8461 ccacgctggt aacaagaact tgattgtccc ctactatctc tcccaaaaga ttagtcctgc
     8521 gagtctcatc caattctgag agaacatcgt ctagaagaag cactggcata tccccagtat
     8581 gcgctcttat gaaatttgcc tcggcaagct tgaccgcaag cactgtcgac ctttgttgcc
     8641 cttgggaccc ataggtagct acgtctgcgc catcaagctg gaaactgatg tcatcacggt
     8701 gtggaccgac catggttacc cctctgatca gctccacctt gcgcaattgt cgcaggcgcg
     8761 cgcgatatct ttcggcaatc cagtcgaccg tcgcctcgct tgttgaacct tgctcgtccg
     8821 cagaaagagg taccgaaggc aagtatctca cggaaagcgc cccaggaact cccgtaatcc
     8881 tgtagtgggc ctcacgcccc agctcgtcaa ggctggctac aacttgcatt cgacgggccg
     8941 ttatcttcga tccggtctcg accaactgct cgtcccatga ttcgagaagc ctgctcgcgg
     9001 cggccgaatc gctccgccaa tttctttccc tagcctgctt cagcagagca tttctctgcg
     9061 taacgatctt ggagtaaagg ttgatcaatt gcctataagc ggggctcacc tgggatatct
     9121 ccagatccag aaacttccgc ctcacagaag ggcttccttt gaccagcgca aggtcgtcgg
     9181 gggtaaaaag caccacgttg agaaaagatg acagctccgc cgcgcgaatc ctttccgtgc
     9241 cgttgagcct cacgagcttt cttccatctg tggaataact gacttccaac gtcacagggg
     9301 cgggcctgcg ctggacgtct acggcgaccc tataatacga actccctttt tggacaagtt
     9361 cggaatcgga tgaggcgcga tatgagcggc ccgcagaggc aacgtacaca gcctccagaa
     9421 ggttagtctt tccttgtgca ttgtgcccca aaaacaggtt gagcccaggg tcgaaagtca
     9481 ccgactcttt gctgtagttt cggaagccac tcaactggat attcctcacc cacacgcttg
     9541 tcacacatcc tccttgccag acccagagct gccgccagat acgatggtaa ccactacatc
     9601 tgcgaactcg aggacgtcgc cgtccctaag tttccttccg cgtctgaact cagcggctcc
     9661 attcaccttc gcccagcccg aatggatggc catcttggcc tctccgcccg atccgaccag
     9721 cgcagcaagt ttgagcgcct ggtcgagctc tatatattca gtctctattt ccaaagtcat
     9781 gtttttcatg cgtcccatct catcacaccg gcgccgttac aggcatcaga agataagtgt
     9841 acgcaggatc cgtctcgtcc gtaacctcta ttggatgcct gtagctggtg tagcctaagc
     9901 acacctcttc cccgtcgagc accctgagca cgtccctcag gtatcttgcg cgcatggcca
     9961 cggtgacctc tgcgtggccg atcatggaca caggtatgtc ttctttgccc tctccgacct
    10021 cgggtgtggc agcggtgatt gtcaaaaagc ccgaggccat tccctcatca ccggtatacg
    10081 acatcctgat ggcgctcaat tcatccttgc acatcagcga taccctgtct acactgtcca
    10141 aaagctgcag gcgatcacag attatctttg ccggtatatt ctccgggaaa acctgcttgt
    10201 aattggggaa ttgtccttcc aatagtcgtg tcacataagt cgtgggccct atccggaaca
    10261 ttgcatggtt tttctcgatg gctatcgaga cttccctctc cccggtgtca ttgaggtttc
    10321 tctccacttc gagaagggcc ttggccggga ttatcacgtc aattggctct tcaagatcga
    10381 acgatgcctg cgttttcctg tatgcaagcc taaatgagtc ggtggccacc atggtcagcg
    10441 agtccttttc gaatagcatg tagatgccct taaaaaaagc cctggcatcg tcgactgccg
    10501 ccgcaggaat ggtgcgcctg atcatggtct tgagctcgct ttctggcata gaccagctat
    10561 ttgcttccga aatctccggc aatgcgggaa actccgcagc cggcatggtt ttcacggtaa
    10621 agtccgctct tccggcggat atgctcacag attggctctc ctcccgatac tcaagaatga
    10681 cttctgactg cggaagcttt ctcgagatat cggagaaata cttggcgggg attactgtcg
    10741 acccctctgt tccaacagta accggaacaa atgtctcgat gcccagttcg tccaggtcgt
    10801 atgctaccaa cctcaggcct tgtgtgcaag tttccacaag cactcctgaa agcgcttgga
    10861 tggtagcctt aggagagacc gccttgtaca cggtctgaac tccgctgacc aaagctgcct
    10921 tggagcatga taccttcacc ttgctatacc tccccactga cctgatttcc aggcacatgc
    10981 atatataagt actactatga gtacatatgt acatttaccg tagtagtagt atacgtgcct
    11041 gtggatatct ggataagtgt cgaatttcat gggttatgct gcccgtctgt tcccagtgtc
    11101 actgtagaca gattgttgat tacttgtgga gcagatggcg agttatcatc agttatccac
    11161 aggggttttt tctttccaca cgctttccac agccttttca catgttgtta actacataat
    11221 caacaggtca actgctcttc aatctggaaa ctatttcctt gatgttcgcg ttcatggttg
    11281 agtccttttt tagctcgttt tgaatcttgt cacaggcgtg gatcacagtc gtgtgatccc
    11341 gtccaccgaa ttcctcgcct atcttcggca gcgaaagctc tgtgagctca cggcaaagat
    11401 acatggcgat ctgccgtggc aaggctacag tgcggaccct cgtctttgac accatttccg
    11461 atacttccag gtggtagtag tctgccactg ttcgctgtat tagctccacc gatatctgct
    11521 ttggccgcga ggatggtatc atgtcctgga ttacctctgc ggcaagctgt gtagttatct
    11581 cgcgattagt cagggcggca caggccgtta ctcggatgag cgctccctcc agctcacgta
    11641 tgttcgaatg tatctggccc gctatataca ccagcacgtc gttgggaacg tcaagaccct
    11701 ccgacacagc tttcttcttc agaattgcga tccttgtctc aaggtcggga gtgcctatat
    11761 ccgtaatcag gccccactca aatcgcgatc gcagccttgc ttcgagcgtg gcaatttctt
    11821 tcgggggtcg atcagacgag agcactatct gttttcccga ctcgtagaga gtgttgaagg
    11881 tatggaagaa ctcttcctga gtgcgttctt ttccggcgag aaactgaata tcatctatca
    11941 tcaggacatc cacgttgcga tatcgctcgc gaaactgaat agtcgtttca aagcgaatcg
    12001 agtttattag ctcatttgtg aattgctcgc aaggaacgta tactaccttg gcataagggt
    12061 tgatctccaa cacatgatgg cctatggcct gcatcaagtg ggtcttcccc aatcccactc
    12121 cgccgtatat gaagagcggg ttgtaggcct ttccaggcgc ttcggaaacg gcaagagcag
    12181 ccgcgtgggc gaacctgttg gagttaccca ctacaaaaga ctcgaaagta tacttcggat
    12241 tgagcgccgg tgtagagaag tcatcgaagg ctttcgcttc agcctgcgtt tgggcgtatg
    12301 ttccagaagt cgctgccgct tcgtcgaccc ccatgcctga tgcggcattt gcgtccaacg
    12361 cgttagcggg cgacggctct cgggtcgcag ggtttgcgtg attgcccatc tcgtaagaat
    12421 cttcgcttac ggctccatct ccgttttctg cctcagccga actttcccta gcaataaacc
    12481 tcaccgttat tttccggccc accatggatg acatggtttc tgacagcaat gctgcgtatc
    12541 gcatttccag ccagtcacgg gcaaagtcgt gcggaaccgg cacttctacg atgtcgtcag
    12601 cgacgacaac ggggacactg gtcttgagcc atgcgtcaaa acttggcctc gacagctcgc
    12661 tttcgaggac ctgaagaaca tccatccaaa ttgcatttat ctcttgggac actttgtcac
    12721 ccctcatttg gaccatcacc taaagacata gctgaggaag tcaacacccc gcctcgtaag
    12781 ctttcgcttg cctccttcga ggctgactac gagtcccaat tcctctaggg tcactagatc
    12841 gcgatatgcc gtgctaaggc ttatgtcaag ctcttttgtg acaaccgaag gccctatctc
    12901 ccccatctct gcaataagca agaacacctt cttctggcgt gaggagagca aggcgccgag
    12961 agcctcatct gacatatcag aggattgaga tgtggatggg ggttcctcat ctcgcctttt
    13021 cttcgactga tctgccgccg aatcctgctt ctcgacggat aaagtggctc tgccttcgtc
    13081 agctatgttt tccatatcaa tggagtcgga ttcgttgtag acggccgaag atgtctgtga
    13141 aatctcggcc cccggatccc gttgtggcct aacgccccct gacccctgag gcccctcggc
    13201 gaagatgtgt ccatctccgt cttgaggatg gccaataaag atgccctgag taggcgggtg
    13261 ataggcgccg gaaatgtcct ggcgcgagct ccacgccgct tctgtaaacc cttgtgcaat
    13321 ctgggctggt tgcatatggg cgggatcatg ccgtacaggc tgggatggac cagcagtctt
    13381 ctgcgaagct ctgtcaggtt tcggcgacac gccgccagga cgtgcgtcgg gagaggaaca
    13441 tagcgtgacc acgagacctg aacccagatt gtcctcgaca gtgaggaaac ctcctaagga
    13501 ctctaaagat tcaatcatta tcgggaaacc agatcccacc ccctttatca acttcctgtc
    13561 gtcggccgtc gctgaggtga aaccgggcat catagcctgg ctcttggcgc gtatgccagg
    13621 tccatgatct gatatgcgca caacattgcc atcgggcatg atcgtaatta ctgcatcctc
    13681 gaatccggcg tgtatcaggt tttcgatggc ttccctcaga acaacaaagg gcatggatcc
    13741 ccctttttct ttgcagaagg cgtataccct ggatgatgct tcatccacaa aggacgcaaa
    13801 gctatcggat gagagatcaa ttgtgcgagg gagaatcgac attgaatcat aagttgctat
    13861 gcgcaatgtg tacgccggag cggactcggg cgctattgcc gatacttcgg cgaccgagtc
    13921 ctctgtctgg cttttctctc cattcatcct gccaaatagg aatttgaaca aagccacctg
    13981 catagcctcc tggcttcgac actctccgct ccactatttc atccacaggt tatcaacatg
    14041 aaaatgtgga taactttagc gccagctaaa atggcccgga attgttcgac aattattgac
    14101 tattgctcga atgctgctag tcgggtttac aagattctcg aaatatcacg ttttcaccat
    14161 aaagttatcc acaacgtttt ccacagctgt tgaaaagtcg tcggaaagcg gagtccgcat
    14221 attgtttata acatatcatc cacaagtatt ccacagagac ctgtggatat cctaccgcca
    14281 gcagattcgg ggcgaacata tgttttagct ggcttttcgc cagtcaaagg cggctttcac
    14341 aattatccac agatggtttt gggaaagttt ttgcccgaat atcggcggtt gtgggaaatc
    14401 cacgaacttc acacaggtta tccacaggga tatccacaga ctgtgcataa aagggcgcat
    14461 aactggcgat aatcattggt gtatgaattc gcgagcgaaa agtcggtatg tttgtacaca
    14521 ctatcataac agagcgagaa gagatcaaat cgagcaacac agcttttcac tactacaacg
    14581 cctcaatgcc cggcaatata tggcgtgaga cagcggaccc tttggcgcgg gtttgcagcg
    14641 ataatgggat gaaactgatc atcgcgggtg atccacaaag aggtaccggc gatcgaggct
    14701 tgataagtcg ggatgcatgt ttgcaagatc cacgagaatc tattgctaag agtgagcgct
    14761 ttggttgcca attgagccga aatgcttctt gcgagttgtc cacagcatgt gaattgtcgg
    14821 ttttttggaa tggacagaag cagatgcgac ttatcagaat gtgacaaact ggagaactca
    14881 aagagtgagg ctgcaataaa taggtaggat cgtggctagt ggccgacttt gtctccgaga
    14941 ttgagagaag atacaatgag ctgcgtgatg gaaggtgcgc agcactaaga tgggctgagt
    15001 gttgaaaagc gcagatgagc aaagatcacg gggaaaggga tatgaggcaa gacaggcttt
    15061 ggggtaatcg tcaaacagtg cccgcctggg ttgatgccct ggagtgtgca ataatcctcc
    15121 tcgaggagac aagcttgggg aggtttatct atgtcaaaga gtaaaggtga atagatgtgg
    15181 aaggcaagga ttgccgaatt caaggctaga tggatgagcg gcaggaatcg gtgcgggtgt
    15241 gcataagaac tgcaaaagga tcttcatggt tctgaaagtg tttgagggac tcctgtgagc
    15301 agcgctcaaa aggcacttgg gggtttgggg cggctgaggc ccgaagaaaa gcaaatgcag
    15361 atacggagat ggcacaacaa ctgaaaatgg atcacattga aaggaatgcg aaactcgcaa
    15421 agatctgttt cggttggcga gccatcttgg atttgaggga cggctgacaa tccgtttccc
    15481 ccatgggaaa agcccgtttt gcagcacgaa aaacagggca accggcccaa agtgtaaaat
    15541 gttgcaaata gtccaaacaa cgtggataga accacaaaag cagttgtttc gctggaatga
    15601 agctgttgag aaattgggcg atttgggaca attgaagctt gttatagact acctgcctga
    15661 cgaagagttg atgcaagcgt tagaaaaagt acggggcaat ggaaggcatc atcttttaat
//
LOCUS       DAOSYL010000053        15667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3146_length_15667_cov_6.026902, whole genome shotgun sequence.
ACCESSION   DAOSYL010000053 DAOSYL010000000
VERSION     DAOSYL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3146_length_15667_cov_6.026902"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..334
                     /locus_tag="PLU15_08405"
     CDS             188..334
                     /locus_tag="PLU15_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30981.1"
                     /translation="MSRLLPRLAQIMPWVPMDELPEALASPLVAAGARPGARRSAWPQ
                     ILVI"
     gene            806..1696
                     /locus_tag="PLU15_08410"
     CDS             806..1696
                     /locus_tag="PLU15_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOO30982.1"
                     /translation="MSQSQYDDELADAILPRPGCLALIRRMFDSLRPAEKKVASYILD
                     NGGQVVYQSITELASATGTSDATIIRFANALGFSGYQELKIALARELVSPGKNIHDDI
                     EPGDSLPVAVNKAFQANIQAVSDTLNVLDIESLQQAVGAVVGARHVYLYGVGTSALSA
                     QDAYYKLLRVGIHANFYADAHMQAISTALITEDDVVIGFSHSGSTKDVVDVLRLAKER
                     GACIVCITNRARSPMAKLADVVLRTASEETPFGSGGMPSMMAQLSVVDALFVGVSLSM
                     YDRAIEFIERTGETVKDKKY"
     gene            1711..3051
                     /locus_tag="PLU15_08415"
     CDS             1711..3051
                     /locus_tag="PLU15_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30983.1"
                     /translation="MFTVGAGLNYIKMPYPVPLAGYQARAELAETQNDPLCARLLTVE
                     GDGGSVAIVALDVLYVDSDLTDLLRTAVSAQTSIPPSSVMVCSTHTHSGPGLIFPNSN
                     RYDSSIVDSIITAVSEAAGRAWSMRTACELSWASGDIVGVASIRNGVTGEAGPANQAL
                     RVAEFRSVDRNLAGVPRFVARAINFGCHATVLGPGNALVSRDWPGFMVDACEEQLRRA
                     GVQRPFVIFLNGAAADVSTRYTRKAQTFEEAARLGRMSADQVFDVFASSGPSRTCHVC
                     LKHESISIPRRQLPSEHCALAAISREEREARRLASCGGSPLALKEALDRAAAWRIVLN
                     RIRHEHGRPRNPLVAHLCLLRLGDLAMVFVPGEAPAKLGDKIRATVAEGASAPEESVW
                     VVGYSNGHLGYISPMRAGPDGYEQLMCDLAPEATEEILTAVARLSSKGMCVDQC"
     gene            3045..3770
                     /locus_tag="PLU15_08420"
     CDS             3045..3770
                     /locus_tag="PLU15_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO30984.1"
                     /translation="MLAQNYLKSVQQTLDQVEKTQLEAIGRAADAIAEAIGNGGRLHI
                     FGTGHSHIIAEEAFYRAGGLVPVNAILEPALMLHDGPFKSTDMERLEGYAEIILDHSG
                     IRPGEVLMIVSNSGRNAVPVEMALTAKARGIIVVALTSLAHSKSVPSRHSSGERLFDV
                     ADIVIDNCGVVGDAVLPVNGLPTNVCPTSTVVGAAIINMIQAEVVERLGAMGIVPPVF
                     VSANVDGGDEIDRQWRGAFEKRA"
     gene            3882..5144
                     /locus_tag="PLU15_08425"
     CDS             3882..5144
                     /locus_tag="PLU15_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO30985.1"
                     /translation="MKRVVTAIVVGLVLAAMIVPVSAAKKVTINFWQAGGDSQTAPVM
                     RQIIKEFEAANPDISVNFQAVPWGEDPHVKFQAAIVGGTIADVFTIGDPFQHVLAASG
                     ALEPLDKYMSKEMKRDFYPQFLERCSVDGKVVALPWFGDVRAMLYRKDLFKAAGVPEP
                     NPSWTWDEFLTYAKKLTRDTDGDGVIDQYGFGTSGRYVSQYQVFLVQNGVNFIDEEKG
                     IATANTPAAIEAMQFYVDLVRKHKVTPPGITTIALQDIQKMFAEGKVAMFFDASDTAV
                     RFSKEPALAGKLGVALLPHNKAHGAFGGSDVIVMSSQSRNKAAAWKFLSFMVSREPML
                     AYCKACGFSPVLKSASTDPYVKADPIRKVYTEQMEIGGFFYFKHPKASSMTPVIRAEV
                     QQAMEGTKSVQAAMNDMQKALEDLLKSK"
     gene            5237..6157
                     /locus_tag="PLU15_08430"
     CDS             5237..6157
                     /locus_tag="PLU15_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO30986.1"
                     /translation="MKSRPVAEKKRVLYRLGEVLESYTMLAPAYVIFLVFIFVPVVWA
                     FYLSFFDYSILSMRTPEFSGIDNFRRVFSDPVFWTALKNTVRYSLGTVPTRLVLGLLL
                     ALALDQANLWGRKVFRTIYFLPVVTSMVAAAIVWSLVFSASNNGLANQFLSLFGVAPK
                     GWLSDSNLAMFSVCVLSVWKDLGYVMTIFTAGLQGIPLELYEAGAIDGATVWQRIRFI
                     TIPLLRPTTFFILVTEIISSFQVFTQTYVMTSGGPGYSTTTLINLLHNKGFVQFDMGY
                     ASALAVALFISLLFLSWVLRKVFKADEIVY"
     gene            6187..7053
                     /locus_tag="PLU15_08435"
     CDS             6187..7053
                     /locus_tag="PLU15_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO30987.1"
                     /translation="MTPKLKKKHTCREIALAGLKYILLVAGAIVMVAPVLWMLSTSLT
                     PDSVIISYKLIPSKITFENYVRAWDFPRVFSDSVTLGTFFFNSVLVSALITGPALIID
                     SLAGYVLARRDIPGKNLLFYLALATMMIPFYVIAVPLFLVVRKLRWLDTFQGMIVPFL
                     SSGFGVFMFKQFFQSVPKELEDAALVDGCSPWRIYWSIMLPLAKPVVGTMTIFKAMWS
                     WNQFFWPLLIINNIKLKTLPLALTMFRGLNVTEWGTLCAGMTIATIPIVVVYLLMQDS
                     FQKGIVMGAVKG"
     gene            7078..8757
                     /locus_tag="PLU15_08440"
     CDS             7078..8757
                     /locus_tag="PLU15_08440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOO30988.1"
                     /translation="MRRTGACRADELLLSMGLREKIGQMTIVGFPERVAGELPEGIES
                     LLKDPGVGGVGIFARNARGPAEMLELTSALQDAAIRAGAKVPLFIMADQEGGMVMQVT
                     DGTTVFPGNMALAATSDAENAHEACRAIALESLAMGINVVTAPDVDVNADPDNPIIGV
                     RAYGDDPALVGRFGSAAVRGYEEGAVLCMAKHFPGHGRTAVDSHLAVPLVDSPIEEVE
                     ALDLPPFEEAVRSGVRAVMTAHIQVPALDTTGACGTLSRPILTELLRGRMGFRGLIVT
                     DCLEMGAIKDTIGTPEAAVRAVEAGADMVLICHTLELQRQAIDGMEQAVLDGRIPREA
                     IDASCRRIITTKLELEEARLRMWTDGRPPLDVVGCRDHLEAEQRIARQSITVLRNDDG
                     VLPFRGDGPKTIAVIYPATMPRLRAEDLAEGDTVLGKEFASRGVTSVEVGVSLCPTRD
                     EIDAALAAAGRADAVVIATSCKTREQEKAQGELVQMLNGAVSAPVVACGIRNPYDIRA
                     YPEVRTYLVTYGYRPCSIRAMVEVMFGEIPAKGRLPVEIPGFYPRGYGLEI"
     gene            8918..10072
                     /locus_tag="PLU15_08445"
     CDS             8918..10072
                     /locus_tag="PLU15_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOO30989.1"
                     /translation="MSTNGRLVLYCVIGCLMLACCAAPVAAKDRKTLAVWMWGSTVRA
                     QGAEAVVKSLAANNVTDVILLVRGTAGKAEYVSAIAPPAEEGKDALGDIIAVCRPLGI
                     RVHAWFVFNQDKSYTDLHHEDRIWHHGKANTDFKPYPVDDGKVCPISQSHLEYTNDMI
                     REVIENYDVDGIHLDYIRYGHAVYCFCPDHVQKATALGINVDRVREVMFETFYASNAN
                     PNRFFEAYESGDADVVAWVNMRQAEIRDAAASMRAAAKLAKPDVIFSASLMPEGAFPE
                     TNSFAMCHYGQSYEDAGELYDFVCPMAYHLDYGKTPAWVGDVARGAAKLVGDDVPVYI
                     GVQAYGEKADRDAVQKAIEAGLGAGAEGVALFRFGTMNEERWSAVTDALK"
     gene            10108..11181
                     /locus_tag="PLU15_08450"
     CDS             10108..11181
                     /locus_tag="PLU15_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HOO30990.1"
                     /translation="MPHPVAMGTVMAEMNVKTFSHTVVGLALIVVMGLVGVKATAQTA
                     EGEGILGLFVPVGTISDGSSAYAEQLRAYRLVNMLMRLGLPAFWIAEPFEYEDDMLPR
                     GSFWVPAPDGTPSVAALAQELADRLGVPYTIVYARRFASGEPRISAFRLRPVSVAVYQ
                     GDRTYSGAADIASSLELVGVYPRYVTDIEIRRGALDDYDILVMPGGAQDVQSYALGPA
                     GKANIRSFVESGGGYLGICAGAYLASAPQFELNLVDAPSQGRGVIGTWIAVRQRPGSD
                     PLFRGYDDMISDLYYGGGPNFVSDHRAIAVLEGVPQSWKPGLQGSAAVIEDTCGIGCA
                     ALVESSSRHCCSRMHIRPGVIAR"
     gene            11114..11851
                     /locus_tag="PLU15_08455"
     CDS             11114..11851
                     /locus_tag="PLU15_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30991.1"
                     /translation="MSNPPHATVVRACISDLESSLDEISYCIGQTCNITARLGDKTER
                     SKFFWNYCYFGWTMKEPARRIRELPAKLSRLADDYEQLEQVSATFEGEAFGFPGYSAG
                     ECMLRSWNAVEAVSRDAAVLQPLWRRISEDMAEIECDVDDLLAKEKAGATPTELDWWR
                     LNIREYLVAIELMGGLNWKYWVSGDSTEEPVRAGYDDEYGDPTQTVAASTTRGAIGTL
                     TMGAMRLDEVLDANLAALAIAEMMLGI"
     gene            11896..12969
                     /locus_tag="PLU15_08460"
     CDS             11896..12969
                     /locus_tag="PLU15_08460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO30992.1"
                     /translation="MDGPRPPLEQELTEVVELVNTVFSPEDRTMGLEFPQLFSGGNLD
                     RLRVFVDQTAGIPVAHGGYVAQELVVEGWRIPVAALGSVCTLPAYRGKGLASRLVESI
                     MDQARSEGLVMMLISGDSNVYKRLGAVETGDFVRVEAHQDQLSYLCEIARAWGDDREI
                     VCRQAGAEDVPSMAATYRREPVRYARSVKDFHELAWWRSDVKRLYAYESIQVACTGRE
                     TLAYIVTRVRTLDGGGVRLHVPEFAGPRWAVACLVGHACTAFSPNVLTMTVCSSDRDM
                     VETLRRAALPLRRTTLPGYTVMALDSSALLSSAGSIISERGGAARDDSLSRLLAISGA
                     DLVKALFGGTDAIPLPVPGLNFI"
     gene            12984..13988
                     /locus_tag="PLU15_08465"
     CDS             12984..13988
                     /locus_tag="PLU15_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOO30993.1"
                     /translation="MDVVIGADGGGTKTACAIAQMDGNIIGLGYAGPSNHLRLPGSHA
                     QAAETVLEGVQAAFSDAGIAVIRARQAYLALGGVDVDRPNEKMAEAARQVLDAEEIAT
                     DNDAAAGLAAATGELYGVVIIAGTGSIAMAVDGEGRRRRSGGWGYRIGDEGSGQWIGR
                     LGLEAAAKAHDGRGKPTSLADRVLDHFGIETVDGLRSVLYKQPVNNRDIADFCMQVMA
                     SAREGDEVALDIVNRAGRELALAVWSAADGLGMASAEFPVVRQGSVFKAGDVIARPFE
                     EELGRLAPAATVVSAAFPPVVGAVMLALMRAGVEIDGAVRENLARSWEEVEARHERLS
                     "
     gene            13972..15159
                     /locus_tag="PLU15_08470"
     CDS             13972..15159
                     /locus_tag="PLU15_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO30994.1"
                     /translation="MSVYLEAAVDMKDEIEEIWTALHGMPEPPFQETKTAGFIAYRLS
                     EAGFDVKKGLADTGIVADLGDPAAGDLTGPVIALRADMDAMRHDIDGREVWVHSCGHD
                     AHSTIVITAGLMLARRCAGARGRLRLIFQPGEETGLGAGRMIEAGAVRGVDALLGMHL
                     RPVSECRVGQATPCLWHSAGRTLSYSVVGKPSHTGRPHLGINVADAVAMATLAVNNVK
                     PDPLRCATVNVVALSAGDGPGGVIPERGSLTVNIRSESDQIADELSSKVDGAVRAVVA
                     ANGAEAGLKREKQMPAPRYDEGMIALARRAIVSVLGEGGVVEKITTPGSEDFHWYARR
                     TPRLHAAYIGLGANFSPGLHHPDASFDKKALVYGAAILAEAAADFLNSGISFEVGCEG
                     RRM"
BASE COUNT         3442 a         3889 c         4789 g         3547 t
ORIGIN      
        1 gaaggaggcg gcagacctcg ctagacgcta cggacagaga taggttcatt cacccggtga
       61 cttgcatctg taacgtaacc cgcgcggaca accgcgctaa ataacttgca gtaagtatct
      121 taatccaatt aggcggccag ggtttggact gcagatgatg cggcggcccc gaggatcgcg
      181 cagaaacttg tcaagacttt tgccaagact tgcccagata atgccgtggg tgccaatgga
      241 cgagctcccc gaggcacttg cgagcccact ggtggcggcg ggcgccaggc cgggcgctag
      301 gcggagcgca tggcctcaga tactcgtgat ttagtcaaac tccatgggaa ccttatggtg
      361 gcctagacaa gtttcaacaa gttcttgcgc ggtcctcgag tccgcgccgc ccgcagtttg
      421 ggctttggtc gcctgatagg gctaggacac tccttgtggc ttgcttgaac ttcatgagac
      481 atgcttatgc atagtccagg gccttcaaac atgctgtttg gccttacatg caccaaatgg
      541 ctatatagaa cacgcaacat gcgaaatgtg ggccaagccc tcggttcagc tatctctagc
      601 agctgctttg tcataacgcg ctccccttcc atctctctgc tgcctcacgc tctagcacac
      661 cttgaagata atctacagaa attattcgta ttctgtggaa ggaatccacc aacatatgca
      721 gaatatctcc actaagacac tgacacatat gaagcaatac tccatacacg gtgtccgacg
      781 aaatagcgca gagggggtga gctacatgag ccagagccaa tatgatgacg aacttgctga
      841 tgctattctc cccaggccag gttgtttggc tctcatacgc cgaatgttcg attcgcttcg
      901 gccggcagag aagaaggttg cctcatacat cttggacaat gggggccagg tagtatacca
      961 gtccattact gaactagcca gcgccacagg cacgtcagac gccacgataa tcaggtttgc
     1021 caatgctctg ggattcagtg gttatcagga actcaagatt gcactggcta gagaactcgt
     1081 atcacccggc aagaacatcc atgacgacat cgagcccggc gattcattgc cagtggccgt
     1141 caacaaggca tttcaggcca acattcaagc tgtaagcgac acgctgaatg tgctcgacat
     1201 tgaatcactg cagcaggcag ttggcgcggt agtcggggcg aggcacgtct atctatatgg
     1261 cgttggtacg tcagcgcttt cagcccaaga tgcatactac aagcttctaa gggtaggcat
     1321 acatgccaac ttctatgcag acgcgcacat gcaagcgatt tcgacggctt tgataacaga
     1381 agacgacgtt gtaatagggt tttcccattc cggaagcaca aaggacgtcg tggacgtgct
     1441 cagacttgcc aaagagaggg gcgcctgcat cgtatgtatt actaatcggg cgcgttcgcc
     1501 catggctaag cttgccgacg tagttcttcg cacagcctct gaagagacgc ctttcgggtc
     1561 aggcggcatg ccatctatga tggcccagct ttcagtggtt gacgcgctct ttgtcggagt
     1621 atcgctgtca atgtacgaca gagccatcga gtttatcgag cgaacgggcg agactgttaa
     1681 ggacaagaaa tactgacggg ggtacgctag gtgtttactg tcggagcggg cctcaattac
     1741 atcaagatgc cgtatcctgt gcctcttgcg gggtatcagg ctagggctga attggcagag
     1801 acacagaatg atcctctctg cgccaggttg cttactgttg aaggcgatgg tggatctgta
     1861 gccattgtgg cgctcgacgt gctgtacgtg gattcggatt tgactgactt gttgcgcact
     1921 gcagtatctg cccaaacttc tattcccccg tcatctgtaa tggtctgctc aacccacaca
     1981 cacagcggcc cgggactgat cttccccaat tccaataggt atgactcctc gatagtggat
     2041 tcaataatca cagcagtatc agaagctgcc ggacgcgcct ggagcatgag aaccgcgtgt
     2101 gagttgagct gggcatcggg agatattgtg ggcgttgcca gcataagaaa tggcgtcaca
     2161 ggtgaggcag ggccggccaa tcaggctctt cgggttgctg aattcaggtc ggtcgatcga
     2221 aaccttgcag gcgtcccaag attcgtggca agagcgatta actttgggtg ccatgctacg
     2281 gtgctaggcc cgggcaacgc attagtctcc cgggactggc ctggtttcat ggtagacgcc
     2341 tgcgaagaac agttgcgccg cgcaggagtg cagcgcccat tcgtgatttt cctcaatggt
     2401 gcggcagccg atgtaagcac cagatacacc agaaaagccc agacctttga ggaggctgca
     2461 aggcttgggc gtatgtcagc agaccaggtc tttgacgtgt ttgcctcctc cgggccctcc
     2521 aggacatgcc acgtatgtct caaacatgaa tccatcagta ttcccagacg acagcttcct
     2581 tcggaacatt gcgcactagc ggctatctcc cgggaggaac gagaggcgcg cagactcgct
     2641 tcgtgcggcg ggagcccttt ggccctaaaa gaggcactgg accgcgccgc agcctggagg
     2701 atcgtgctca acaggataag gcacgagcat ggcagaccgc gtaatcccct tgttgcccac
     2761 ctgtgcttgc ttaggctggg tgatctggcg atggttttcg tgccgggtga agcgcctgcc
     2821 aagttaggcg acaagatccg cgccacggtg gctgaaggtg ccagcgcacc agaggagagt
     2881 gtgtgggtgg ttggctattc aaatggccat ctgggataca tatcgccgat gcgggcgggg
     2941 ccggacgggt acgagcagct gatgtgcgat cttgctcctg aagcgacaga agagatacta
     3001 acggcagtgg ccagactttc gagcaaaggg atgtgcgtcg accaatgtta gcccaaaact
     3061 acttaaaaag cgttcaacag accctggatc aggtagaaaa gacacaactc gaggcgatag
     3121 gcagagccgc tgatgcaata gccgaggcaa ttggaaatgg cggaagactg catatcttcg
     3181 gaactggcca ctcccacatc attgcggaag aggcgtttta cagggcgggc gggcttgtgc
     3241 cggtcaacgc aatactcgaa ccggcgttga tgcttcacga tggtccgttt aagtccacag
     3301 acatggaacg gttggaggga tacgcggaga tcatcctgga tcactccggc attcgccctg
     3361 gcgaggtctt gatgatcgta tcaaattcag gccggaacgc tgttccggtg gagatggctc
     3421 ttactgctaa ggcgcgcggg attattgtag tggctcttac atcccttgcg cactcgaaga
     3481 gcgttccctc ccgccattca agcggagagc gcctttttga cgttgccgat attgttatag
     3541 acaactgtgg tgtcgtaggc gatgccgtgc tgccagtgaa cggtctgccc acgaatgtct
     3601 gccccacatc cactgtggtg ggagctgcaa tcatcaacat gattcaggcg gaagtagtgg
     3661 aacgcctagg cgccatgggc atcgtgcccc cggtgtttgt aagcgccaac gtggatgggg
     3721 gcgatgagat cgaccggcag tggcgggggg cctttgagaa aagggcttga gcaacaattt
     3781 tgaggttagg aggtggatga tggcaatagg ctttgcacac ggcagttctt ccgatgcgcg
     3841 atttagtcgt ttccataaaa aatgatcagg aggcttgaac aatgaagcgg gttgttactg
     3901 ccattgtagt aggtctagtt cttgcggcca tgatcgttcc cgtgagcgcg gcgaagaaag
     3961 tcacaatcaa cttctggcaa gctggcggag actcccaaac agccccggtg atgaggcaga
     4021 tcatcaagga gtttgaggct gcgaaccccg acatcagtgt caacttccag gctgtaccgt
     4081 ggggagagga tccacacgtc aagttccagg cagccatagt tggtggaact attgctgatg
     4141 tgttcacgat cggagatccc ttccagcatg tgctggctgc aagcggcgcc ttggagcctt
     4201 tggacaaata catgtcgaag gaaatgaaac gagacttcta ccctcagttt ctggagagat
     4261 gctcggttga tggcaaggta gtggcactgc cgtggtttgg cgatgtccgt gccatgctct
     4321 accgcaaaga cctattcaag gcggccggcg tacccgaacc caatccatca tggacatggg
     4381 atgagttcct aacctatgca aagaagctca ctcgcgacac agacggtgat ggcgttatcg
     4441 accagtatgg attcggcacg tcaggccgtt atgtctcgca gtaccaggtg ttcttggttc
     4501 agaacggagt caacttcata gatgaagaga agggcattgc taccgcaaac acccctgcgg
     4561 caatcgaggc aatgcagttc tacgtggatc tcgtgagaaa gcacaaggtg acccctccgg
     4621 gcatcaccac aatagcgttg caggatattc aaaagatgtt cgcagagggc aaggttgcga
     4681 tgttcttcga cgcgtctgac acggcagtca ggttctcaaa agagccggcg ctcgctggca
     4741 agctcggtgt ggcattgctt ccccataaca aggcacatgg agcgttcggc ggttctgacg
     4801 tgattgttat gtccagtcag tccaggaaca aggcagctgc gtggaaattc ctatcattca
     4861 tggtatcccg agaaccaatg ctcgcgtact gcaaggcatg cgggttctcg cctgtgctca
     4921 agtcggcctc tacagatccg tatgtaaagg ctgacccaat tcgaaaggta tacacagagc
     4981 agatggagat aggcggcttc ttctacttca aacaccccaa ggcctccagc atgactcctg
     5041 tgatcagggc tgaagtgcag caggctatgg aaggcactaa gtcggtgcag gcagcaatga
     5101 atgacatgca aaaagcgctt gaggacttgc tgaagagcaa gtagaggcta ctacggttgt
     5161 cctccgccgg acccacgttg accgtggtgt cgggtccggc ggaggacagg actccgggag
     5221 aggggagacg gtgactatga aatctaggcc tgtggctgag aaaaagcgtg tgttgtatcg
     5281 actcggggaa gtcctggagt cctatacgat gttggcgccg gcctacgtga tattcctggt
     5341 attcatattc gtccccgttg tatgggcgtt ctatctcagt tttttcgact atagcatctt
     5401 gtcgatgagg acccctgagt tttcagggat tgacaacttc agacgggttt tctccgatcc
     5461 ggtattctgg actgcgctca aaaacaccgt aaggtactcg ctggggacgg tgcccacgcg
     5521 tcttgtcctc ggtctgcttc ttgctctcgc gctagatcaa gcaaatctat ggggacgaaa
     5581 ggttttccga accatctact ttcttcctgt tgtaacatca atggtcgccg ccgcaatcgt
     5641 ctggtcgctg gtattcagcg caagcaacaa cggtcttgcc aatcagtttc tcagcctctt
     5701 cggcgtggcg cccaaagggt ggctctctga ttcgaatctc gccatgttct cagtttgcgt
     5761 gctgagcgtg tggaaggacc ttggatatgt gatgaccata ttcacagcag gtttgcaggg
     5821 cattcccctg gagctctacg aggcgggggc aattgacggc gcgacggtat ggcagagaat
     5881 aaggtttatc actatacctc tattgaggcc cactacgttc ttcatacttg tcaccgagat
     5941 tatcagctcg ttccaggtat tcacgcagac ttatgtcatg acttcaggcg ggccgggata
     6001 ttcgactacc actctaatca acctgcttca caacaagggc tttgttcagt ttgacatggg
     6061 atatgcaagc gcattggctg tggcattgtt catttcattg ctcttcctga gctgggtgct
     6121 tagaaaagtg tttaaggcag acgagatcgt atactaggct tcaaagtcga acggagggcg
     6181 atgaacatga ctccaaagct aaaaaagaag cacacgtgca gggagatagc tctggcagga
     6241 ctcaagtata tactgcttgt agcaggcgcc attgtgatgg tagctcctgt gctctggatg
     6301 ctgtcaacct cgctaactcc agactcggtc attatctctt acaagcttat tccttcgaaa
     6361 atcacatttg aaaactacgt tcgcgcctgg gatttccccc gggttttcag cgactctgtg
     6421 actctgggaa cattcttttt caacagtgtg cttgtgagtg ccctaattac tggtcctgca
     6481 ttgattatag actcgttggc cggatatgtt ctggcgcgaa gggacattcc cggcaagaac
     6541 ctcctattct acctagccct ggccacgatg atgattccct tctacgtcat tgccgtgcct
     6601 ctcttccttg ttgtgaggaa actacgttgg ctcgacacat ttcaaggcat gatcgtaccg
     6661 tttctttcgt cgggcttcgg agtattcatg ttcaaacagt tcttccagtc agtgccaaag
     6721 gagcttgagg atgcggcact tgtggacggc tgttcgccat ggagaatcta ctggtcaatc
     6781 atgctgccgc tggcgaagcc ggtagtcgga acgatgacta tcttcaaggc catgtggtcg
     6841 tggaaccagt tcttctggcc ccttctcatt atcaacaaca tcaagctgaa gactttgccg
     6901 ttggcgctca ccatgttccg gggtttgaac gtaacagaat ggggcacgtt gtgtgccggc
     6961 atgacaattg ctacgatccc aatagtcgtt gtatacctgc tcatgcagga ctcgttccag
     7021 aaagggattg tgatgggtgc agtcaagggc tgagccaagc aaaaggggga tttcatcatg
     7081 agaaggactg gcgcgtgtcg agcagatgag ctcttgctgt cgatgggctt gcgcgagaag
     7141 atcgggcaga tgaccatcgt gggattcccg gagcgcgtcg ctggtgaatt gcctgaaggg
     7201 atagagtcgc tcctgaaaga ccccggggtg ggcggtgtcg gcatctttgc ccgcaacgcg
     7261 cgcggacccg ctgaaatgct ggagcttaca tcagccctcc aagatgccgc gatcagggca
     7321 ggggcgaagg ttccgctttt catcatggcg gatcaagagg gcggcatggt catgcaggtg
     7381 acagatggaa ctactgtctt cccgggcaac atggctcttg cagcgacgag tgatgcagaa
     7441 aacgcgcatg aggcttgccg ggcaatcgcc cttgaatctc tggccatggg cataaacgtt
     7501 gtgacagctc ctgatgtaga tgtgaatgct gatcctgata acccgataat tggggtaagg
     7561 gcctatggtg atgatcctgc gttggttggg cggttcggaa gtgccgcggt gcgcggatac
     7621 gaggaaggcg cagtgctgtg catggccaaa cacttccccg ggcatggacg gactgctgta
     7681 gattcccatc ttgcagtgcc gctcgtagat tcgcccatcg aagaggttga agccctggat
     7741 ctgccacctt ttgaggaagc tgttcgctct ggggtacggg cggtgatgac ggcgcacatt
     7801 caggttccgg ctctcgatac aacaggcgcg tgcgggacat tgtcacgtcc gatcttgact
     7861 gaacttctcc gcgggcgcat gggatttcgt ggcctgatag tcaccgattg cttggagatg
     7921 ggggccatca aggacacaat aggcacgccc gaagccgcgg ttcgggcagt agaggcagga
     7981 gctgacatgg tcctgatatg tcacactctt gagctgcaga ggcaggcgat tgacggcatg
     8041 gagcaggcgg tgctggatgg gcggattccc agggaggcca tagacgcctc gtgcaggcgg
     8101 atcatcacta ccaagctcga gctcgaagag gctcgcctga ggatgtggac agacggacgt
     8161 cctcctctgg atgttgtggg atgcagggac catcttgagg ccgagcagag aatcgctcgt
     8221 cagtcgatca cggttttgcg aaacgacgac ggagtgcttc ccttccgcgg cgatggaccg
     8281 aaaacgattg ctgtcatcta ccccgccacc atgcccaggc ttcgggcgga ggacttggcg
     8341 gaaggtgaca cagtgctcgg caaggagttt gcgtcgcggg gcgtgacttc ggttgaagtg
     8401 ggcgtaagtc tgtgccccac tagggacgag atcgatgcag cccttgcggc ggcaggacgc
     8461 gcagatgcgg tagtgatcgc aacatcctgc aaaacccggg aacaagagaa ggctcagggt
     8521 gaactcgtgc agatgctgaa tggagcggta agtgcgcctg ttgtggcgtg cggcattcga
     8581 aacccctacg atatcagggc atatccggaa gtcagaactt acctcgtcac ctacgggtat
     8641 cggccatgtt ccatcagagc catggtggaa gtgatgtttg gcgagattcc ggcgaaggga
     8701 aggcttcctg tggagatacc gggattctat ccccgtgggt acgggctcga gatctaggtg
     8761 gatctcgaat agttttgcat gatcgtggaa tctttggcat ccaacattcg agctttagct
     8821 agcaaagaca gctcagcatt gtttgctatt cactcgattt ggcgcatctg gcgccgatcg
     8881 agacgaagac aatttgaaga gggaggctcc tgaagctatg tcgacgaatg ggcgactcgt
     8941 gttgtactgc gtaatcggat gtctcatgct ggcttgctgc gcagctcctg ttgcggcaaa
     9001 ggaccggaag actttggcag tatggatgtg gggaagcacc gtgagggcgc agggcgctga
     9061 agctgttgtg aaaagcttgg ctgcaaacaa cgtgaccgac gtgattctgc tggtgagagg
     9121 aacggctggc aaggccgaat acgtcagcgc aatcgcgccc ccggcggagg agggcaaaga
     9181 cgctctgggc gacatcatcg cggtctgtcg cccactgggg atccgagtgc atgcttggtt
     9241 cgtgttcaac caggacaagt cctacaccga tctgcatcat gaagatagga tctggcatca
     9301 tgggaaggcg aacactgatt tcaagccgta tccggtggat gacggaaagg tttgccccat
     9361 ttcccagtcc cacctggagt acacgaatga catgatcaga gaagtgatcg agaactacga
     9421 cgtcgacgga attcacctgg attacatacg ctacggccac gcggtctact gtttttgccc
     9481 cgaccacgtt caaaaggcaa ctgccctggg aatcaacgtc gatcgggtcc gcgaggtcat
     9541 gtttgagact ttctatgcaa gcaacgccaa tccaaaccga ttcttcgagg catacgagtc
     9601 tggcgacgca gatgtcgtcg cctgggtcaa catgcgccag gccgagatac gcgatgccgc
     9661 ggccagcatg cgcgccgccg ccaagctggc aaagccagat gtgatctttt ctgcaagcct
     9721 gatgcctgaa ggcgccttcc cggagacaaa ctcctttgcg atgtgccact atggtcagag
     9781 ctatgaggac gccggagaac tttacgattt cgtctgcccc atggcctatc atcttgatta
     9841 cggaaaaacc cctgcctggg ttggggatgt ggcacgtgga gcagctaagc ttgttggcga
     9901 tgacgtgcca gtctacatag gagttcaggc ctatggcgaa aaggccgatc gtgacgcggt
     9961 gcaaaaggct atcgaggccg gccttggggc aggggccgag ggcgttgctc tgttcaggtt
    10021 cggcactatg aacgaagaac gatggtcagc ggtgactgac gctctgaagt agtcgcaagc
    10081 ggagcaaaga ggcaaggggt ggagctgttg ccccaccccg tcgctatggg gacggtgatg
    10141 gcggagatga atgtgaagac gttctctcac actgtagtgg ggttggctct gattgtcgtc
    10201 atgggtctgg ttggggtgaa ggctacagcc caaacggcgg agggcgaagg gatactcggc
    10261 ctgtttgttc ctgtagggac gatatctgac ggctcttcgg catatgcaga gcagcttcgc
    10321 gcctacaggc ttgtgaacat gcttatgagg ttaggccttc cggcattctg gatagctgag
    10381 ccctttgagt atgaggacga catgcttccg cgcggcagtt tctgggttcc cgcgcctgac
    10441 ggcacccctt cagttgcagc gcttgctcaa gaacttgcgg acaggctagg agtcccctat
    10501 acaatcgtct atgccaggcg cttcgccagc ggcgaaccac ggatttcggc tttccgcctc
    10561 aggcccgtaa gcgttgcggt ctatcagggc gatcggacat actcgggcgc tgctgacata
    10621 gcttcatctc tggagctcgt cggtgtgtat ccacgttatg tgacagacat cgagatcagg
    10681 cgtggagctc tcgatgatta tgacatcctt gtgatgcctg gaggggctca ggatgtgcag
    10741 tcatacgctc ttggacctgc ggggaaggcg aacatacgct cgtttgtgga atctggcggt
    10801 ggctatctgg gcatctgtgc aggggcgtat ctggcgtcag cgccacaatt tgagctgaac
    10861 cttgtagacg ctccatctca aggacgagga gttataggga cttggatagc tgttcgtcaa
    10921 cggccgggca gcgatccgct gttcaggggc tatgatgaca tgatatccga tctttattat
    10981 gggggtggac ccaatttcgt ttctgatcac cgggcgatag cagttctcga aggagttcct
    11041 cagtcttgga aacccgggct ccagggatca gctgcggtca tcgaggatac atgcgggatt
    11101 ggttgcgccg ctcttgtcga atcctcctca cgccactgtt gttcgcgcat gcatatccga
    11161 cctggagtca tcgctcgatg agatcagcta ctgcatcggc cagacttgta acatcacggc
    11221 tcgccttggg gataagactg agcgatccaa gttcttctgg aattactgct acttcggatg
    11281 gaccatgaag gagccggcaa gacgaatacg cgagcttcca gcgaagctta gccggctggc
    11341 agacgattat gagcaactcg agcaggtttc cgcaaccttt gagggtgaag cattcgggtt
    11401 tccaggctac agcgcgggcg aatgcatgct tcgctcctgg aatgcggtgg aggctgtctc
    11461 cagagatgca gctgtattgc agccgctgtg gagaagaatc tccgaggaca tggcagagat
    11521 cgaatgcgat gtcgatgatc tccttgcgaa ggagaaggca ggggctacgc ctaccgaact
    11581 ggactggtgg aggctgaaca tcagagagta tctcgtggca atcgagctta tgggtgggct
    11641 aaactggaag tactgggtaa gcggggatag cacggaggag cccgttcgcg ccggctacga
    11701 tgacgaatac ggcgatccta cgcaaactgt agccgcctca acgacgcgcg gggcgatagg
    11761 taccctcaca atgggcgcca tgcggctcga cgaggttcta gacgcaaatc tcgcagctct
    11821 cgccatcgca gagatgatgc ttggcatctg atccttccgg gggcggccat acgcactaag
    11881 aagggagcac atcaaatgga tggacctcgt cctcctctcg aacaagagct tacagaggtc
    11941 gtagagcttg taaacacagt cttcagccct gaagatcgca ccatggggct cgagtttccg
    12001 cagctgtttt ctggaggcaa tctggatcgg ctcagggttt tcgtcgatca gacagcgggg
    12061 atccctgtgg ctcatggagg atatgttgca caggagttgg tggttgaagg ttggcggatt
    12121 cctgtggcgg cgctcggatc agtctgcact ctgccagcct atcggggcaa gggccttgcg
    12181 tcgaggcttg tggagagcat tatggaccag gcgcggtccg aggggcttgt catgatgctc
    12241 atatcaggcg actccaatgt atacaagagg ctgggcgctg ttgaaaccgg cgatttcgtg
    12301 agggtagagg cacatcaaga tcaactttcc tatctgtgcg aaattgcgcg ggcgtggggt
    12361 gacgacaggg agattgtgtg caggcaggca ggagcggagg acgtgccgtc gatggcggcc
    12421 acatacaggc gtgaaccagt gcgatatgca agatcggtca aggattttca tgaattggcc
    12481 tggtggaggt ctgacgtcaa gcgtctttac gcctatgaga gcattcaagt ggcatgtacc
    12541 gggcgggaaa cgctggctta cattgttaca cgcgtgcgca ccctggacgg cggaggagta
    12601 aggctgcatg tgcccgagtt cgcggggcct agatgggctg tggcttgtct ggtggggcat
    12661 gcctgcacag cgttttcgcc caacgtgctt acgatgacgg tttgctcatc agatcgcgac
    12721 atggttgaaa cgttgcggcg ggctgcattg cccttgcgta gaacaacgtt gcctggctac
    12781 acagtgatgg cgttggattc ttctgcactt ctgagtagcg ctggatccat catttccgaa
    12841 aggggcggcg ctgcgcggga tgattcgctg tcgcgactct tggcgatctc aggtgccgat
    12901 cttgtgaagg cgttattcgg gggcacggat gcgatcccgt tgcctgttcc gggtttgaat
    12961 ttcatatagc cggagggttt tgaatggacg ttgtaatagg cgctgatggc gggggaacca
    13021 agaccgcctg cgccatagca caaatggacg gaaacatcat tggactgggt tacgctggac
    13081 catccaatca tctgcgcctg cccggaagcc atgcacaagc ggcagagacc gtgctcgaag
    13141 gcgtacaggc agcattttcc gatgcgggga tagcagttat ccgggctcgc caggcctacc
    13201 ttgcgcttgg aggcgttgat gttgatcgcc caaatgagaa gatggcagag gcggcccgcc
    13261 aggtcttgga cgcagaagag atagcaactg acaatgatgc ggcggcaggc cttgcggctg
    13321 ccacgggtga gttgtacggt gtggtcatca ttgccggaac aggaagtatt gccatggctg
    13381 ttgacgggga aggcaggcgg cgtaggtctg gcggatgggg gtatcgcatc ggagatgaag
    13441 gtagcggcca gtggatagga cgcctgggac ttgaagctgc ggctaaggct catgacggcc
    13501 gtgggaagcc aacatcctta gcagacaggg tgttggatca tttcgggata gaaaccgttg
    13561 atggactccg atctgtcctc tataagcagc cggtaaacaa cagggacatc gccgatttct
    13621 gcatgcaggt aatggcttcg gcccgggagg gcgatgaggt tgccctcgac atcgtgaacc
    13681 gggcagggcg cgaattggcc cttgccgtgt ggtctgctgc agatggactt ggcatggctt
    13741 cggccgagtt tccagtagtt cgccagggaa gcgtgttcaa ggcgggcgat gtcattgcgc
    13801 gtcctttcga ggaagagctt ggccggctcg cgcccgcagc cacagtggtc agcgcggcat
    13861 ttccgccagt ggttggggcg gttatgctgg cattgatgcg ggcaggcgtt gagattgacg
    13921 gtgctgtgcg ggaaaacctc gcaaggtcat gggaggaggt tgaggcgcgc catgagcgtc
    13981 tatcttgagg cagctgttga catgaaggac gaaattgaag agatttggac tgcactccat
    14041 gggatgccgg agcctccatt tcaggagacg aaaactgcgg gtttcatagc atacaggctt
    14101 tcggaagccg ggtttgatgt gaagaagggc cttgcggata cggggatagt ggctgactta
    14161 ggagatcctg ctgcgggtga tctgacggga ccggtgattg cccttcgggc cgatatggac
    14221 gcaatgaggc atgacataga tggacgcgag gtttgggttc attcctgcgg tcacgacgct
    14281 cattcgacaa tagtcataac ggcaggactc atgctggccc ggcggtgtgc cggggccaga
    14341 ggcaggctaa gattgatctt tcagcccggg gaagagacag ggctgggcgc tggcaggatg
    14401 atagaagcgg gcgcagtccg gggagtagat gccttgctcg gaatgcacct gcggcctgtg
    14461 tcagagtgcc gtgtggggca ggcaacacct tgtctttggc attcggcagg gagaaccctt
    14521 agttactcag tggttgggaa accgtctcac acggggcggc ctcaccttgg gataaacgtg
    14581 gcggacgctg tggctatggc aaccttggct gtaaataatg tcaaacccga tccgcttagg
    14641 tgtgcgactg tcaacgtagt tgcgttgagt gcgggagatg ggccaggcgg cgtcataccg
    14701 gagaggggaa gcctgacggt gaacatcaga agcgagtctg atcagatcgc agatgagctt
    14761 agttccaagg tggatggggc ggtcagggct gtcgtggctg ctaatggcgc cgaggcaggg
    14821 ctgaaaagag aaaagcagat gccggcccca cgctatgatg agggcatgat cgctctggcg
    14881 cgccgggcta tagtctcggt gctgggagag ggaggcgttg tagagaagat cacgactcct
    14941 gggtctgagg attttcactg gtatgcaagg agaactccaa gactgcatgc cgcttacatc
    15001 ggccttgggg ccaacttttc gcccggactc caccatcctg atgcttcgtt tgacaagaaa
    15061 gccctggtgt atggggccgc gatactggct gaagccgcag ccgatttcct gaatagcggc
    15121 ataagcttcg aagtgggatg cgagggacgt agaatgtaga gtacaatgcc ttctatgcac
    15181 tactcggaca cggcgtttgg tccaccacgt tatagataat tatgtagaat atgtgatatg
    15241 cggaatgtga gatgaacaat ggctcccatt ttgttctcgg gcgagctgaa taaaagcaag
    15301 gggtatgcct gttagctggc taatgttctt cgttcggctt ttgtgcaatc gctttttgcc
    15361 ggacataggc tatgcggttg cttgtgaata agggggttaa gcttctgagc gcggatttcc
    15421 gatgcttgcg cgctggtgac ctggatcgcg tcattttcgc ccgtccacgt aactcgaaag
    15481 ttcaagaatt gaaagccgag ccgtcacgcg ttcatcttct tagaatgcgt ctcttgcatc
    15541 agggatcacg ctcatgctta cgactcgcgt cccctgatat ggctgaaagg tcgagaccga
    15601 aaagctatgt taagctcgac atatccgaaa tggctatttc gggcatgttt tgcgcggaat
    15661 acaagaa
//
LOCUS       DAOSYL010000054        15280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3254_length_15280_cov_8.077373, whole genome shotgun sequence.
ACCESSION   DAOSYL010000054 DAOSYL010000000
VERSION     DAOSYL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15280
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_15280_cov_8.077373"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLU15_08475"
     CDS             <1..193
                     /locus_tag="PLU15_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30995.1"
                     /translation="RILFYLQEPRRIAGALCFSSVKNIEGGLIMEHCDDLAAVMYLEY
                     VEEAGGPKWFDWHIRAVLS"
     gene            257..2365
                     /locus_tag="PLU15_08480"
     CDS             257..2365
                     /locus_tag="PLU15_08480"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOO30996.1"
                     /translation="MDLGALAFLVPLSAAIALVVAAGLSASIKRKDEGTEKMRQISEA
                     IREGASAYLRRQYRGVILFFAVMFGVLCMLAAMDFLNWFTPFAFLSGGFFSGLAGYIG
                     MRTATQANARTAHAASKSLNEGLQVAFSSGCVMGLTVVGLSLVHLSLWYYLLTWYYSS
                     AQPIADAAQRLSAITSTLLTSGMGASSMALFARVGGGIFTKAADVGADLVGKVEQGIP
                     EDDPRNPAVIADNVGDNVGDVAGMGADLYESYLGAIIATSALGATVGLGVNWVAFPMV
                     LAAIGVFASIIGTTFVRTGEEVEQSLLLAALRRGVNVSAIIVVVASWFAVRAMLGPDF
                     MGIFWSVIAGLVAGVVIGRVTEYYTSADYKPTKAVAASATTGPATVIISGMASGMAST
                     AIPVLVVAVAVIASYYLAGGSSNEVMGLYGIAISAVGMLSTLGITLATDAYGPVADNA
                     GGIAEMAGMPENVRERTDALDSLGNTTAATGKGFAIGSAALTALALIAAYRDQILLLD
                     PGRELVFSLMTPSVLVGVFIGGMLPFIFSALTMQAVGRAAQGIVMEVRRQFQEMPGIM
                     RGKTKPDYARCVDMCTSSAQKEMIAPSLIAIIVPIVSGIFLGPEGVGGVLVGTVVTGF
                     LLAVMMANAGGSWDNAKKHIETGEYGGKGSDAHKASVVGDTVGDPFKDTSGPSLNILI
                     KLVSMVSIVFASLIISLSIM"
     gene            2703..2948
                     /locus_tag="PLU15_08485"
     CDS             2703..2948
                     /locus_tag="PLU15_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO30997.1"
                     /translation="MSHGALLGIERATEILRQLADGLTDTVVIKARLGYFDQQLALLS
                     VGKTLEGDVDRRVACIDGRGRGMAVKGRHPVERILCV"
     gene            3002..4546
                     /gene="gpmI"
                     /locus_tag="PLU15_08490"
     CDS             3002..4546
                     /gene="gpmI"
                     /locus_tag="PLU15_08490"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOO30998.1"
                     /translation="MQTYRPVVLAILDGWGYSENEFYNAIALARTPNIDKLMQDYPYT
                     TLPAHGRAVGLPEGQMGGSEVGHLCLGSGRIVLQDLSYLDHKIETGEFQRNEVLLGAM
                     KEAARKGKALHLMGLVSPGGVHSHQKHIYTLVEMARDVGVRDVYIHAFLDGRDVPPAS
                     ALDYLDELDANLTRLGLGKVATVSGRYYAMDRDNRWDRTKLAWDAIVHSVGPRARAAQ
                     DAVRASYDSGITDEFVMPTVIVDESGAPVGPVREGDSVIFFNFRGDRARQLTKAFNFE
                     EFSEFDRGERPHVHYVCMMKYLNADIPVAFSPPEPVDGLAETISKAGKAQLHVAETEK
                     FAHVTFFFNGGREEPFPNEDRVLIPSPKVATYDLAPEMSAREIAAEVVSRIEAGKYDF
                     IVVNFANGDMVGHTGKLDAAIAAVETVDDCVGQVWGAVKEARGAMIITSDHGNADQML
                     DPDTGQPSTAHSLNKVPFILAGTQATQLRDGGTFADVAPTILELMGIQPPAAMTGRSL
                     IAGYGE"
     assembly_gap    4720..4819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5110..6630
                     /locus_tag="PLU15_08495"
     CDS             5110..6630
                     /locus_tag="PLU15_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO30999.1"
                     /translation="MDKEKYLTGADPLDIPLEQFDAPLFNKLLWYVKEDPVRFHMPGH
                     KGVGYPPEVLEAFSENMLSMDVTETRDLDNLHLPTGCIKQAQDLASEVFGADRTFFLT
                     NGTTAGIHAMLMTSCKEGQKVIIPRDAHLAVVGGVILAGLAPVFVYPDIDPEWGISMG
                     VRPEAYEQALTENPDAKACLITRPNYYGIAGDLAPLAEICHQRDIPLLVDEAHGPHIR
                     FHEDLPAPALEYGADMVVQSTHKTLGSFTGSSMLHIKGPRIHPDRVQRMLAILQSTSP
                     SYLLMASIDIAATIMRLRGPQLIDRALKLSDKARRRLDGILGIRVLWRPGMDTLRLTV
                     SMIQLGLAGYDLKNRLMDECNVRIELADLENIVAFITYADSEENVDRLVDALLQIADE
                     ARSEMTGKLGVRTSGNQAEFTRHLFAPAERACLPRQATLAPHELVEVKDAVGRVCAEV
                     VAPYPPGIPLLYPGEIIDGLHLDIIEEALSLGAVFRGLSFGPDGQLIVTVVKDGKR"
     gene            6630..7277
                     /gene="tmk"
                     /locus_tag="PLU15_08500"
     CDS             6630..7277
                     /gene="tmk"
                     /locus_tag="PLU15_08500"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOO31000.1"
                     /translation="MTQRGVFITLEGPDGSGKSTQAGLLAERLESEGYPVTRTFEPGG
                     TSIGKRLREIILDNKSTGMCGVAEFLLYAADRAQDVHEVIEPALEAGRIVVAERFVDS
                     SIAYQGYGLGMDIDEVKQVNRIATRGLVPDLTLLLDIDPAIGVRRATDCHEADRIEQR
                     ELDFHVSVREAYLMMAAAEPERFMIIETGGLCVLEVHEEIYTAVTKLLETRAWED"
     gene            7281..8372
                     /locus_tag="PLU15_08505"
     CDS             7281..8372
                     /locus_tag="PLU15_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO31001.1"
                     /translation="MHIPEIVGQDEALRLLDREMASGRMSHAYLLWGPRGSGKMALAK
                     RVALAANCMNCGAGNAAGDRSERGPDSPLWYCGNCSNCEKILRGVHPDVRVVKPEGGA
                     IKIDQIREMKWRISLKPNEADLKTWIIDEADRMTEEAQNCLLKVLEEPPGDSLIILAA
                     EDPGMMLPTISSRCHEARLKAVPVEELARWGQSELGLDPERARLLATLSEGLPGRLVC
                     LAQDDDYFKVRDLIISTVRAVALCGNGAEALAASEEFLDKLKKASAQAVDGGGSVEVQ
                     ELASPMGACDLAAGWFRDLFILSITGDESLMINIDRIMEIREDVRKGEPCLFSRWTVK
                     AMQTAQALRSSANPRLAMDDFFLNLALPE"
     gene            8388..9203
                     /locus_tag="PLU15_08510"
     CDS             8388..9203
                     /locus_tag="PLU15_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOO31002.1"
                     /translation="MVKIVGVRFKKAGKVYYFDPGELDLAAGDDVIVETARGVEFGQV
                     VIGPKFVDEEEIVQPLKQVIRGATDADRRQVEENKKKAERAFDIGLEKIAAHGLPMKL
                     VDVEYTFDNSKIVFYFTADGRVDFRELVRDLASVFRTRIELRQIGVRDEAKMLGGIGS
                     CGRALCCASFLGEFEPVSIRMAKEQNLSLNPSKISGICGRLMCCLKYEINPVRSETED
                     MPELGDRVITPSGPGKVMSIDPETRSVRVQLDSGGHASISLTELEYECADKEE"
     gene            9333..10097
                     /locus_tag="PLU15_08515"
     CDS             9333..10097
                     /locus_tag="PLU15_08515"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOO31003.1"
                     /translation="MSGGRADETREALLHDYERLDDLQRSGLVIIQNPAGFRFSMDAV
                     LLAEFATVRPRDRAIDLGTGTGVIPLLVWARRQPAEIVGIEILEEMADMAKRSVQING
                     LSDRIKIVHGDLRDAVDLFGAGYFDVVLSNPPYIKVDEGTLSPEGHLTVAKHEVSCTL
                     EDVVKVAAGLVKPRGRVAMIHRASRLADLLSSMREANLEPKRLRLVQARVGAPPMMVL
                     VEAVKDVKAGLQVMPTLMLYGEDGQYSEETKQIYFG"
     gene            10148..11059
                     /gene="rsmI"
                     /locus_tag="PLU15_08520"
     CDS             10148..11059
                     /gene="rsmI"
                     /locus_tag="PLU15_08520"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOO31004.1"
                     /translation="MTRYGEAAEKGTLYLVATPIGNMDDITDRARAVLAEADIVAAED
                     TRHTGLLLHRLGIDARLLSCHEHNEAARVEQIIAMLGRGEDVAVVSDAGMPGISDPGE
                     IIVAAAVEGGFKVVPVPGPTAFVAALTASGLPTSRFFFQGFLPRKVGDRRKVLEELSS
                     LPVTLIFYEAPHRLERMLEDVADILGDRRCVVARELTKKFEDIQRGTVGEMLEWVRER
                     KPKGEIVVIVEGCSQRSAATEGCPEGNAEDELEMAVAEVAQLVGEGFTMRDAVSKVAE
                     ERGISRREVYARAHEERDGGRKKQKGT"
     gene            complement(11135..11380)
                     /locus_tag="PLU15_08525"
     CDS             complement(11135..11380)
                     /locus_tag="PLU15_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOO31005.1"
                     /translation="MKSTGIVRKVDELGRVVIPIELRRTLQINEKDSLEIYVDGEKII
                     LKKYEPACIFCGNADGIRIFKDKNICKDCFDLMKSVG"
     gene            complement(11553..12209)
                     /locus_tag="PLU15_08530"
     CDS             complement(11553..12209)
                     /locus_tag="PLU15_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31006.1"
                     /translation="MSSYAGSFRECPFKIGRKPNNRTKFAFLFLAVFSIATAICPPAL
                     CQKVQSQVVGDLTLAVATPVGVDLDIAYADSFSFRIPLNAGYLIYERADSQGVRHLCL
                     SDYNQSGIDFELIVDLFPIDAQSPTSLDALARGLLYAGARATAIDSKVVEISGRPGFD
                     LTVKGIVYDRIVPQDEIRMVFLTDFDSGLTFTLIEHQHRPEDHANMLDKILSTLIFTD
                     "
     gene            12407..14470
                     /gene="metG"
                     /locus_tag="PLU15_08535"
     CDS             12407..14470
                     /gene="metG"
                     /locus_tag="PLU15_08535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOO31007.1"
                     /translation="MTKEPFFVTTPIYYVNDMPHLGHSYTTIGCDALARYKRMCGYDV
                     FFLTGVDEHGQKIERRATAEGKSPQEYVDYVTDAFKRLWAELDISNDGFIRTTDPEHV
                     RAVQHVFQQLYDSGDIYKGKYEGWYCTPCEAFFMESQLVDGNCPDCGRPVEWLQEESY
                     FFRLSKYEDRLLAHIEANPDFIQPATRRHEMVNFIKSGLNDLCVSRTTFDWGVPVPFA
                     PGHVVYVWLDALINYLTASGYVDDPERFAKYWPADVHVVGKEIVRFHSVIWPAVLMSL
                     GLPLPKKVFGHGWWTVEGAKMSKSVGNVVKPHDYAKEFGVDAVRYFVLREVPFGQDGD
                     FSRASFVYRVNADLANDLGNLLSRTTAMINKFAGGKVPEPGEWDDADKKIPEIATDAV
                     DKAARAFDDLAFSEALENVFRLVDAGNKYIDDVEPWNLAKTDAGRRRLGTVLYNLAES
                     LRIAAVALSPVLTSKSAEIWYQLGIDGDPAEAGWEATKWGGTVPGTEVRRGDPIFPRI
                     DVKAALKEEASSESGSASSREGSGGRVKGESVQCCGGDFGEGHAQPDGGKAVLRAAAA
                     PARDGEALDADNIISIDDFAKVDLKVGTVLEAERIEGADKLLVLQVDIGSETRQIVAG
                     IAKHYSPEELIGKQIVVVTNLKPAKLRGVMSNGMLLAASTTGDPKEVRVVTPDGPVPA
                     GSRVK"
     gene            14474..15250
                     /locus_tag="PLU15_08540"
     CDS             14474..15250
                     /locus_tag="PLU15_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOO31008.1"
                     /translation="MIIDSHAHLEDEAFVEGVYAVMERARAAGVEMVINPGADVRSSR
                     VAAENAAVHEGLYAMAGIHPHDAAGVGDGDLEEISRILSMPRVVGIGEIGLDYYRDLS
                     PRDEQIRVFRAQLELAKEFGCPVQIHSRDAHRDTFEILKEYADDVPAIVLHCYSGSPE
                     MAAELIELGFFISLGGPVTYRNARKPVEVAKSVPLERLLVETDSPYLPPSPYRGKRNE
                     PAYVVEVVRKIAEIRGVSTEEVARVTAANAMRIFSISPEG"
BASE COUNT         3448 a         3596 c         4631 g         3505 t
ORIGIN      
        1 ccggattctt ttttatcttc aagagcctcg gcgtattgcc ggggctcttt gcttttcttc
       61 agtcaaaaac atagaagggg gcctgatcat ggagcattgt gatgaccttg cggcggtaat
      121 gtacttagag tatgtggaag aggctggcgg gcccaaatgg tttgactggc atattcgggc
      181 tgtgttatcc taggattgtt agttagctgc acggaggggg ggaattacac ccgatgttcg
      241 tgtcgggtgg agggtaatgg acttaggagc attagcgttc ttggtgcctt tgagcgcggc
      301 aatcgcattg gttgtagcag ctggcctgtc tgcctccata aagcggaagg atgagggaac
      361 cgaaaagatg cgccaaatct ctgaagctat cagggagggc gcatctgcgt atttgaggcg
      421 gcagtataga ggggtcattc tattttttgc agtaatgttt ggtgtgcttt gcatgctggc
      481 tgcaatggat tttttgaact ggtttacgcc ttttgcgttc ctgagcggcg gttttttctc
      541 cggccttgcc gggtatatcg gaatgcgcac tgccacacag gctaacgctc gaactgccca
      601 tgctgcctcg aagagcctga atgagggttt acaagtcgcg ttttcgtcag gctgcgtcat
      661 gggtctgact gttgtggggt tgtcccttgt tcacttaagc ttatggtatt atctccttac
      721 gtggtattac agctctgcgc agcctatcgc agatgctgct cagcgcctat cagcaatcac
      781 ctccacactt ctcacgtccg gaatgggagc aagctcgatg gcgctctttg ccagggtggg
      841 cggaggcatt ttcaccaaag ctgcggatgt tggcgctgac ttagtcggaa aagtggaaca
      901 aggcatacct gaagacgatc cgcgcaaccc cgctgtcata gccgataatg ttggtgataa
      961 tgtgggcgat gttgcaggaa tgggcgcaga cctttacgag tcgtatctgg gtgcgataat
     1021 cgccacctca gccttgggtg cgaccgttgg gttgggagtg aactgggtcg cattcccgat
     1081 ggtcctggcg gcgatagggg tgttcgcatc gattattgga accaccttcg ttcgcacagg
     1141 cgaggaggtg gagcagtcgc tgttgctagc ggcactccgc cggggcgtga acgtaagcgc
     1201 gatcatcgtg gtggtagcga gttggtttgc ggtgagggcg atgttggggc ctgattttat
     1261 gggcatattc tggtcagtca ttgcaggcct cgttgcaggc gtagtaatcg gccgggtcac
     1321 tgaatactat acatcggcag attacaagcc aacaaaggcc gtggctgctt ccgccactac
     1381 cggtccggct acagtcataa tatctggcat ggcttctggc atggcttcga cagccattcc
     1441 tgtcctggta gttgctgttg cagtcatcgc cagctactat ctggcgggtg gatctagcaa
     1501 cgaggtaatg ggcctttacg gaatcgccat atcagctgtg ggaatgctct ccacgctcgg
     1561 aatcacattg gctacggatg cttacggtcc cgtggctgac aatgcagggg gcattgcaga
     1621 gatggcgggc atgccagaaa atgtgaggga gaggacagat gctctggatt ctctgggcaa
     1681 cactacggct gccacgggaa aggggtttgc catcggttcg gcagccctta cggcgctggc
     1741 tcttatcgcg gcgtatcgag accagattct tctgctcgac ccaggccgtg agctagtctt
     1801 cagtctgatg accccgtctg ttctagttgg ggtgttcatc gggggcatgc ttccgttcat
     1861 attctccgcc ctcacaatgc aggcggttgg aagggcggcg cagggaattg tcatggaggt
     1921 tcgcaggcag ttccaggaga tgcctggaat aatgcgcgga aagacaaagc ccgattacgc
     1981 caggtgtgtg gatatgtgca cttcatctgc gcagaaggaa atgattgcgc catcgctcat
     2041 tgccatcata gtcccgatcg tcagcggcat ctttctcggg cccgaggggg ttggaggagt
     2101 cctcgtgggt accgtggtca caggattctt gcttgcggtg atgatggcta atgcgggcgg
     2161 gtcctgggat aatgccaaga aacacataga aaccggcgaa tacggcggaa aaggctctga
     2221 tgcacataag gcaagcgtag tcggtgacac tgttggagac ccgtttaagg acacatctgg
     2281 tccgtcattg aacatactta tcaaacttgt ctccatggtg tcgatagtct tcgcttcctt
     2341 gataatatct ttgtccatca tgtaaagcgc gcgcctgtta cccgcgggcg gtcctttcgt
     2401 gatcggacgg tgccggcctg ctccttgagg agtaggccgg ttcatactgt acatgataga
     2461 tttcagataa gcacctgctc tgctgcgagc caagcagaac ggccatttca ataaagtgaa
     2521 tgcaacttgg caggggtgta tgtcctagtc aaatgactcg ctagcgggcc gctaagccgt
     2581 aaaatagctt tcaggcaatg cacccgactg gttggccctg acggtttccc aaaggatgcc
     2641 acatatccgc ccaaaactca aatcccgaca caaatgggga tgtcaaatgg aaaggtcccc
     2701 aaatgtctca cggggcttta ctaggcattg aacgcgcaac ggaaatcctt agacagcttg
     2761 cagacggtct tacggatacg gtagtgataa aagcaaggct aggctacttc gaccagcaac
     2821 tggcacttct gagtgtaggc aaaactcttg agggagatgt tgatcgcaga gttgcttgca
     2881 tagatggacg gggtagaggc atggccgtga aggggcgaca tccagttgaa cgcatattgt
     2941 gcgtgtgata cgataatcct gctgtcagct gggaatctca gctgcttggg ggtgtaaatt
     3001 gatgcaaaca tacaggccag tcgttcttgc aattttggat ggttggggct actcggagaa
     3061 tgaattctat aatgccatag ccttagcccg aaccccgaat atcgacaagc taatgcagga
     3121 ctatccctat actactcttc ccgcccatgg gcgcgctgtg ggacttcctg agggacagat
     3181 gggcggttcc gaagtcgggc acctatgcct cggttcagga agaatagtcc tccaggacct
     3241 cagttacctg gatcacaaga tagagacggg cgagtttcag cgaaatgagg tcttgctcgg
     3301 ggcgatgaaa gaggcagctc gcaaaggcaa agctcttcac cttatgggcc ttgtgtctcc
     3361 aggcggggtg cattctcatc aaaagcacat atacacgctg gttgagatgg cgcgtgacgt
     3421 tggcgtcagg gatgtatata tacatgcctt cttggacggg agagatgttc cgcccgcaag
     3481 cgccttggac tacctggatg agctcgatgc caaccttacc cggcttgggc ttgggaaagt
     3541 ggcaacggtc agcggacggt actatgccat ggatcgggat aaccgctggg ataggactaa
     3601 gcttgcgtgg gacgcaatag tccattctgt gggcccccga gctcgcgctg cacaggacgc
     3661 tgtcagagct tcatacgatt cgggtataac agatgaattc gtgatgccca cggtgattgt
     3721 ggatgaatca ggtgctccag tggggccggt gcgcgaagga gactcagtaa tattcttcaa
     3781 cttccgcggg gacagagcca ggcagctcac gaaggctttc aactttgagg agttcagcga
     3841 gtttgaccgc ggagaaaggc cccatgttca ttacgtgtgc atgatgaaat acctaaatgc
     3901 cgatatccca gtggcatttt cgccgccgga gccagtcgat gggctggcag aaacgatctc
     3961 gaaggcgggc aaggctcagc tgcatgtggc cgagacagaa aagtttgccc acgtcacgtt
     4021 cttttttaac ggaggccgcg aggaaccatt ccccaatgag gacagggtgc tcatcccgtc
     4081 tccgaaagtg gcaacgtacg accttgcgcc tgagatgagc gccagggaga tagctgccga
     4141 ggtcgtgtcg agaatagagg cggggaagta cgattttatc gttgtcaatt ttgccaacgg
     4201 cgacatggtt gggcacacag gcaagctcga cgctgccata gcagctgttg agactgtgga
     4261 tgattgcgtt gggcaggtat ggggagcagt aaaggaagcc cggggcgcaa tgatcatcac
     4321 ttctgaccat gggaacgccg atcagatgct ggaccccgac actgggcagc caagcacagc
     4381 tcattccctc aacaaggtcc cgtttatttt ggcaggcacc caagcaacgc aacttcgcga
     4441 cggtggaacc tttgcagatg tggcccctac gattctagag cttatgggga ttcagccgcc
     4501 tgcagctatg actggaagat cgctcattgc gggatacgga gaataatgaa actgaaccgt
     4561 cttgtagcta tacgccaagg ctcttgcctg aaggcatgtg ccgatttggg agccggttgg
     4621 gcttcgacgc ttcttcataa cgtcaactgc agcagggcag tccgagtaga ttatgagttc
     4681 tcgggcgcgg tgtttccgtc cgatagtgag ggccatgagn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnng tgcggccgga gtggatatgc ggcttcggat accactttgc
     4861 cagagctgtt ttctgcgagc agcacagctt tccatgggcc cctatcgtga ctgccgcttg
     4921 agacggctgc aacgtatatt cgcaatgggc gtgccatgtt gttacaaccc cctaactctt
     4981 gggcaacaag gagaacacat gttctacgac tagatattaa catagaacgt atgttcgtgt
     5041 caagcctacc ctaaagttgt gccaataatc cccgggcacc ggggcataaa ggagaagata
     5101 agatcagaag tggacaaaga gaaatattta acaggcgcag atccactcga tatccctcta
     5161 gagcaattcg acgccccgct tttcaacaag ttgctttggt acgtcaaaga ggatcccgtt
     5221 cgttttcaca tgcccgggca caaaggtgtg ggctatcctc cggaggttct ggaggccttc
     5281 agcgagaaca tgctcagcat ggatgtgacc gagacgcggg acctggataa tctccatctt
     5341 cctaccggat gtataaagca ggcgcaggat ctggcatcgg aggtattcgg agctgaccgg
     5401 acgttttttt tgactaatgg aaccaccgca ggaatccatg ctatgttgat gacaagctgc
     5461 aaggaaggac agaaagtgat catcccgcgt gatgcccact tagctgtggt aggtggtgtg
     5521 atcctggctg ggctggcgcc tgtcttcgtg tatcccgaca tagatcccga gtggggaatc
     5581 agcatggggg tgaggcctga agcatacgag caggctctca ctgagaatcc cgatgccaag
     5641 gcttgcctga tcacgcgccc caattactat ggcatagcag gagaccttgc gccgttggca
     5701 gaaatctgtc atcaacgcga tattccattg cttgttgatg aggctcatgg accgcacata
     5761 cgctttcacg aggatcttcc ggctcccgcg ttggagtatg gcgcagacat ggtggtccaa
     5821 agcacccata aaacgctcgg ttcgtttacc ggaagctcca tgctccacat caaggggccc
     5881 cggatccatc ccgatcgcgt tcagcgtatg ttagcgatac tccagtccac tagtccgtca
     5941 tacctcctca tggcctcgat tgacatcgct gctacaatca tgaggttgcg cgggcctcag
     6001 cttatcgata gagcgctcaa gctttctgac aaggctcggc ggcgtctgga tgggattttg
     6061 ggcataaggg tgttgtggag accgggcatg gatacgctta gactgacagt ctcaatgatc
     6121 cagttgggat tggctggata tgatctaaag aaccggttga tggatgagtg caatgtcagg
     6181 atcgaacttg cggacctcga gaatatagtc gcttttataa cctatgccga ctctgaggaa
     6241 aatgtggatc gccttgtaga tgcgcttttg cagattgccg acgaggcgcg cagcgagatg
     6301 acaggaaagc tcggcgtaag aacctcggga aaccaggcag agttcacccg gcatctgttt
     6361 gcgcctgcgg agagagcttg tcttcctagg caggcaactc ttgctccgca cgaactggtc
     6421 gaagttaagg acgccgtcgg cagggtatgc gccgaggtgg ttgccccata tccgccgggg
     6481 ataccgcttc tttatccagg agagatcatc gacggcttgc atctcgatat catagaggaa
     6541 gcgttgtccc ttggggcggt cttccgaggc ctttcgttcg gcccagatgg gcagctcatc
     6601 gtgaccgtgg tgaaagatgg gaagagatag tgacacaacg tggagttttt ataaccttgg
     6661 aagggcctga tggcagcgga aagagtacgc aggccggtct attggcggaa cgccttgaat
     6721 cagaagggta tccggtaacg cgcactttcg agccgggcgg cacgtctata gggaagagat
     6781 tgcgggagat catcctggac aataagtcca caggaatgtg cggggttgca gagttcctgc
     6841 tttatgctgc agatcgtgca caggatgtgc atgaagtaat cgagccggct ctagaggcgg
     6901 gcaggatagt tgtggcggag cgttttgtgg attccagcat cgcctaccaa ggatacggtc
     6961 ttggcatgga tatcgatgaa gtgaagcagg tgaaccggat agccactcgc ggcctggttc
     7021 ccgatcttac gttgctgctc gacatcgatc ctgccatcgg tgtcagacgt gcgaccgatt
     7081 gccacgaagc cgacaggatc gagcagcgcg agctcgattt tcatgtgtca gtccgcgagg
     7141 cttacctaat gatggccgct gcggagccgg agcgtttcat gattatcgag actggcggac
     7201 tatgtgtttt ggaagtgcac gaggagatat acactgctgt gacgaagtta ctcgaaacgc
     7261 gtgcgtggga ggactgaggg atgcacattc ctgaaatcgt cggtcaggat gaggccctcc
     7321 gattgctcga tagggagatg gcttcggggc gcatgtccca cgcttatctt ctgtgggggc
     7381 cacggggaag cggcaagatg gcgctggcaa aacgggttgc tctcgctgca aactgcatga
     7441 actgtggggc ggggaatgca gccggagacc gatctgagcg cgggccggat agcccactgt
     7501 ggtattgcgg caattgttcg aattgtgaga agatactgag aggcgttcat ccggatgttc
     7561 gtgtggtgaa accggaggga ggcgccataa agatagacca gatcagggag atgaagtggc
     7621 gcatatccct taagcctaat gaagccgacc tcaagacatg gataatagat gaagcggatc
     7681 gcatgactga agaggcccag aactgcctcc tcaaagtgtt ggaggaaccc cctggtgact
     7741 cgttgatcat ccttgcggca gaggatccgg gcatgatgtt gccgacaatc tcatcgcgtt
     7801 gccatgaggc acgcctcaaa gctgttcccg ttgaagagct tgccagatgg ggccagagcg
     7861 agctcggtct cgatcctgaa cgggccaggc ttctggctac gctttcggaa gggcttcccg
     7921 gccgcctggt atgcctggct caagacgacg actatttcaa ggtaagagat ctcataatat
     7981 ccacagtgcg cgcagtggcc ttgtgtggga atggtgccga ggcgcttgct gcttcggagg
     8041 aattcctcga caagctgaag aaggctagtg cgcaagctgt agatggagga gggtccgtgg
     8101 aggttcagga gctcgcttct ccaatggggg catgcgacct tgcggccgga tggttcagag
     8161 acctcttcat cctgtccata acaggtgacg agagtctgat gataaacatc gatcgcatca
     8221 tggagattcg cgaagatgtg cgcaaaggcg agccctgctt gttcagtcga tggacagtca
     8281 aagcgatgca gactgcacaa gcgctcagga gcagcgcgaa tccgcggcta gccatggatg
     8341 acttcttctt gaacctggcc ttgcctgaat gaaagggtgt ccgcaacatg gttaagatag
     8401 ttggagtgcg gttcaagaaa gcgggaaagg tatattactt cgaccccgga gagctcgatc
     8461 ttgcggcagg tgatgacgtc attgtcgaga ctgcccgcgg cgtcgaattc ggccaggtgg
     8521 tgatcgggcc caagtttgtg gatgaggaag agatagtaca gcccctgaag caggtgataa
     8581 ggggcgcgac tgacgctgat aggcgccagg ttgaggagaa caagaaaaaa gcggaacgag
     8641 ccttcgatat tgggctcgaa aagattgcgg cgcatggcct tcccatgaag cttgtggatg
     8701 ttgaatacac gttcgacaac tcaaagatag tcttctactt tactgctgac ggacgtgtcg
     8761 acttcaggga gctcgtcagg gatctggctt ctgtcttccg aacccgcatc gaactgaggc
     8821 agataggggt gagggatgag gccaagatgc ttggagggat cgggtcgtgt ggacgcgcgc
     8881 tttgctgtgc ctctttcctt ggggagtttg agcctgtatc gatacgcatg gcaaaagagc
     8941 agaatctgtc gcttaatccc tccaagattt ctggaatctg tggaaggctc atgtgttgtc
     9001 tcaagtatga gataaatcct gtaaggtccg aaactgagga catgccggaa ctgggcgata
     9061 gagtcatcac tccttcgggc ccgggcaagg taatgtcgat agaccctgaa acgcgttctg
     9121 tcagggttca actggatagt ggggggcatg cgtcgatctc tctgacagaa cttgaatacg
     9181 aatgtgcgga taaggaggag tgacgccgcc gtcggctctg aggcaaaccc ggtgctttag
     9241 gcggtgtgca tgttacttgg tgaaaggggg atatcgcgca cgacccaatc atgcgcgggc
     9301 agattgcatg attgtcgtca ggcgtacggc ttatgagtgg cgggagggcg gatgaaacgc
     9361 gcgaggcgct gctgcatgac tacgagagat tggacgacct gcaacggtcc ggacttgtga
     9421 taattcagaa tcctgccggg ttcaggtttt ccatggatgc cgtgctcctt gcggaattcg
     9481 cgactgttag gccgcgggac agggctatag atcttggcac aggcacaggc gtgattcccc
     9541 tcctggtctg ggccaggcga cagccggcgg agattgtcgg cattgaaatt ctggaagaga
     9601 tggccgacat ggcgaagcga agtgtccaga tcaacgggct ttccgataga ataaagatcg
     9661 tccatggcga cttgagggac gctgtagacc tttttggcgc aggctatttc gatgttgtgc
     9721 tatcaaatcc cccatatatc aaggttgatg agggaactct gagccctgaa gggcacctaa
     9781 cagtcgccaa gcatgaagtg tcgtgcactc tggaagacgt ggtgaaagtg gctgcaggac
     9841 ttgtgaagcc aaggggtaga gtcgctatga ttcaccgtgc atccaggctt gcggatcttc
     9901 tttcatccat gcgcgaggca aatctggaac cgaagcgtct ccggctggtg caggccaggg
     9961 tgggcgcgcc gcccatgatg gtattggtcg aagcggtgaa ggatgttaag gctgggctgc
    10021 aggtaatgcc cactttgatg ctttatggag aggacgggca gtattctgaa gaaacaaaac
    10081 agatatactt cggctaagag acgggcgaaa cttggggaca tgtagagttg gtggagggtg
    10141 atcgccggtg acacgctacg gcgaagctgc cgaaaagggt actctttact tggtggccac
    10201 gcctattggg aacatggatg acattaccga cagagccagg gctgttctgg ccgaagcgga
    10261 tatcgtggcc gctgaggata ccaggcatac cgggctcttg ttgcatcgcc tgggaattga
    10321 cgcaaggttg ctgtcgtgcc atgaacacaa cgaagcggct cgggtggagc agatcattgc
    10381 gatgctgggc aggggcgaag atgtggccgt ggtctctgat gcagggatgc cgggaatctc
    10441 tgatccggga gaaatcattg ttgctgctgc ggtggagggc gggttcaaag tggtacctgt
    10501 tccgggcccc acggcgttcg ttgcagcgtt gactgcatct ggattgccga caagcagatt
    10561 cttttttcaa ggctttcttc ctaggaaggt gggggatagg cgtaaagttc ttgaagagct
    10621 ttcttcattg ccggtcactc tcattttcta cgaagcgccg catcgactcg agcgcatgct
    10681 tgaagatgtg gctgacattc tcggcgacag aagatgtgtg gtggcgcgag aacttactaa
    10741 gaaattcgag gacatacaga gaggcaccgt aggcgagatg ctggagtggg tgagggaacg
    10801 caagcccaag ggagagattg tagtcatagt cgaagggtgc agccaacgaa gtgcggcgac
    10861 agagggttgc ccggaaggga acgccgagga cgagttggaa atggctgtgg ccgaggtggc
    10921 gcagctcgtg ggcgagggtt ttacaatgag ggatgctgtt tccaaggtgg cagaggagcg
    10981 tggcatctca aggcgcgagg tatatgcgcg tgcacatgag gagcgggatg gcggtcggaa
    11041 aaagcaaaaa ggcacttaaa gtgccttttt gccagacgaa cgttgctatt tgcagtgctt
    11101 tcgcccggat ggggcgaggc aagtcgagaa ccgactagcc tacgcttttc atcaggtcga
    11161 agcaatcctt gcagatattc ttgtccttga agatcctgat tccatcagcg ttgccacaga
    11221 atatgcaggc aggttcgtac ttcttcagga tgatcttttc cccgtcaaca tagatttcca
    11281 aggaatcctt ctcattgatc tgcaacgttc ttcgtagttc gatgggtatt acgactcttc
    11341 ctagttcgtc gacttttcta actataccgg tagatttcat tttcgcgacc ctcccacgca
    11401 aatactcggc aatgcctgcc aaacaaataa tagtatcttt tccaagagga gtcaagtact
    11461 aatttcacaa attcttgggt tttacgctgc cgatttgggc aaaacagtca tattccgtgc
    11521 acttttgcct aacaaaagca ttttttactc cgttaatcag tgaatatcag ggtagataag
    11581 attttatcca acatatttgc atgatcttcc ggacggtgct gatgctctat gagggtaaac
    11641 gtgagtcccg aatcaaaatc tgtaagaaag accatccgaa tctcatcttg cggcacgatt
    11701 cggtcgtaga cgattccctt gacggtgaga tcaaatccgg ggcgccccga tatctccacg
    11761 actttagaat cgattgccgt tgctcttgct cccgcgtaaa gcaagcctct tgcaagagcg
    11821 tccaatgatg taggtgattg ggcatcaatt ggaaagaggt cgacgatgag ctcgaagtcg
    11881 atcccgctct ggttgtaatc ggaaaggcat agatgacgga cgccttggct atctgccctc
    11941 tcgtagatca ggtatccggc atttaatggg attctgaagg agaagctatc ggcataggca
    12001 atgtcgagat caaccccaac aggcgttgcg acggccagcg ttaggtcacc aacaacttga
    12061 gactgcacct tttggcagag ggcgggaggg cagatcgctg ttgcaatgct gaacacggcc
    12121 aagaagagaa acgcaaattt cgtgcgattg ttcggtttgc gaccgatttt gaatggacat
    12181 tctcgaaacg accccgcata gctggacaat cttttcacct cgctattaaa ataccacata
    12241 atgggctggc gttcaacagt aaggcattcc ttgacagttc acggtggctc acggtatatt
    12301 caacttagcc tttaccaggc atgctagctt ttgttttgcc ccctgtgtgt gtggcggaaa
    12361 ctcagcggca gagcagaggt tcttgtctat tttggaggga ctgtttatga caaaggaacc
    12421 cttttttgtt actacaccta tatactatgt gaacgacatg ccccaccttg gccattcgta
    12481 tacgacgatc ggctgtgacg cgttggctag atacaagcga atgtgcgggt atgacgtatt
    12541 tttcctcacg ggcgtcgacg aacatgggca gaagatcgaa aggcgcgcca ccgcggaagg
    12601 gaagagtccc caggagtatg tggactatgt gacggacgcc tttaagagac tctgggcgga
    12661 gcttgacata tccaatgacg gcttcatccg cacaacagat ccggagcacg tgcgggcggt
    12721 tcaacacgta tttcagcaac tgtacgattc tggagacatc tataaaggga aatatgaagg
    12781 atggtattgc acgccgtgcg aggcattttt catggagtcg cagcttgttg acggcaattg
    12841 tcctgactgc gggcgacctg tggaatggct gcaggaggag agttatttct tccgtttgtc
    12901 gaaatatgag gaccggcttc tcgcgcacat agaggctaat cccgatttca tacagcctgc
    12961 aacgaggcgc catgagatgg tcaatttcat aaagagcgga ttgaacgatc tttgcgtgtc
    13021 gcgaaccact ttcgattggg gtgttcctgt gccttttgcg ccgggacacg tcgtctacgt
    13081 ttggctggat gccctcatca actatttgac cgcgtcgggc tatgttgatg atcccgagag
    13141 gttcgccaag tattggcctg ctgacgttca cgttgttggc aaggaaatag tcaggttcca
    13201 ttctgtgata tggcctgcgg tgctgatgtc tttgggattg cctctcccga aaaaggtatt
    13261 tggccatgga tggtggacgg tggaaggggc gaagatgtcg aaatccgtgg gaaacgtggt
    13321 aaagccgcat gactatgcga aggagtttgg agtggacgca gttcgctact tcgtcctccg
    13381 cgaggttccg tttgggcaag acggggattt ctcccgcgca tcctttgtat acagggtcaa
    13441 tgctgacctc gcaaacgatc tcggaaatct gctcagccgc accacggcga tgataaacaa
    13501 gtttgccggt ggaaaagtgc ctgaaccggg agaatgggat gacgctgata agaagatccc
    13561 cgaaatcgct accgacgctg tcgacaaggc tgcccgggcc ttcgatgatt tggcgttcag
    13621 tgaagccttg gagaatgtat tcaggctcgt tgacgcagga aacaagtaca tcgacgacgt
    13681 tgagccatgg aatcttgcca aaactgatgc tggccgcagg aggctgggca ctgtccttta
    13741 caacctggcc gaaagcctaa ggattgcggc cgtggcttta tcgcccgtgc taacaagcaa
    13801 gtcagccgaa atctggtatc agctgggtat tgacggagac ccggcggagg cgggatggga
    13861 ggccaccaag tggggcggca cagttccggg caccgaggtt agacggggtg acccgatttt
    13921 ccctaggatt gatgtgaagg cggcattgaa ggaggaggcg tcgtcagaga gcgggagcgc
    13981 atcctcgcgc gaaggctctg gtggcagagt gaagggtgaa tcagtccaat gctgcggtgg
    14041 ggacttcggc gaagggcacg cgcaaccgga tggcgggaag gctgttttaa gggctgcagc
    14101 tgcgccggct cgcgacggtg aagcgttgga cgccgacaac atcatatcca tagatgactt
    14161 cgcaaaggtc gatctcaagg taggtactgt gctcgaggcc gagcggatcg agggcgccga
    14221 caagcttctc gtgcttcagg tggacatcgg ctccgagacc aggcagatag ttgcgggcat
    14281 tgcgaagcat tactctcctg aggagctcat aggcaagcag atcgtggttg tgactaatct
    14341 gaagcctgcg aaactgcgcg gcgtcatgtc aaatggaatg ctgcttgcgg catcgaccac
    14401 aggtgatccc aaggaggtta gggttgtgac cccggatggc ccagtgcccg ctggaagcag
    14461 ggtgaaatag gcgttgatta tcgattccca tgcccatctt gaagatgagg cttttgtaga
    14521 aggcgtgtac gccgtgatgg agagggcgcg cgcagccggc gtcgagatgg taatcaatcc
    14581 tggcgcggat gtgcgctcgt cccgtgtggc tgctgagaat gcggccgtac atgaaggact
    14641 ttacgccatg gccgggatcc acccccatga tgcggccgga gtgggcgatg gtgatctgga
    14701 agagatatcg aggatccttt cgatgccccg tgtagtgggg attggcgaga tagggctcga
    14761 ctattacaga gacctttcgc cgagagacga gcagatcagg gtctttcgag cccagctcga
    14821 gctcgcgaag gagtttggat gcccggttca gatccatagc cgcgatgccc atcgtgacac
    14881 cttcgagatc ctgaaggagt acgcagacga tgtgcctgcc atagtgctgc attgctactc
    14941 gggcagccct gagatggctg cggaactgat cgaactcggg tttttcatat cgctgggcgg
    15001 ccctgtaacc tacaggaatg ctcgaaagcc ggtggaagtt gcgaaaagtg tgccgcttga
    15061 gcgtctcctg gtggagactg attcgccgta tcttcctcca agcccgtacc ggggcaagcg
    15121 aaacgaacct gcatatgtag tggaagttgt acggaagatt gccgaaatca ggggcgtatc
    15181 gacggaagag gtagctcggg tcacggctgc caatgccatg aggatattct caatatcacc
    15241 cgaaggatga cgcggaaatc gcccgcaagg gtgacaggga
//
LOCUS       DAOSYL010000055        15214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3272_length_15214_cov_8.602480, whole genome shotgun sequence.
ACCESSION   DAOSYL010000055 DAOSYL010000000
VERSION     DAOSYL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15214
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3272_length_15214_cov_8.602480"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      66..288
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            446..2218
                     /gene="nrdD"
                     /locus_tag="PLU15_08545"
     CDS             446..2218
                     /gene="nrdD"
                     /locus_tag="PLU15_08545"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOO31009.1"
                     /translation="MLEAQHEIHEAGHGESKWLDLMNAIGEIIENTSRENANIGNSPS
                     AKMLQIASSASREYYLDRVIPDDIACAHRNGDIHIHDLDYYGKTLTCVHIPLGRLLAE
                     GFDTGHGYVRPPKRPASAAALAAIIIQSCQNDMHGGQSFAHFDTDMGPYMEGFDEDEA
                     YQGMEAFIYNLNTMHSRAGAQVPFSTVNIGCDTSAGGRRVTRLLLEAYERGLGRGETP
                     VFPNIIFRVKSGVNMNPKDPNYDLFKLACRVCARRMNPTFSFMDSSFNAPYGDEVAYM
                     GCRTRVMANRRGQETSVGRGNLSFTTINLPRLALMAGGNVDRFFRLLDTKLELVYRQL
                     LHRFDIQSRLKVRDMPFLMGQKIYIDSYGLGENDEIGPAIANGTLSVGFIGLAETLTV
                     LCGAHHGENEEAEQLGREIVSFMRRQIDGFAEESNLNFTLLATPAEGLSGRFVAIDRE
                     RFGTVRNVTDREYYTNSFHLPVRYPTTISRKLVVEGAFHGLTNAGHISYVELSSPPIE
                     NGEAVESIVKAMARADMGYGGINFPIDECRKCGHAGVIESSCPSCGSGDISRIRRITG
                     YLAPLDRFNDAKKRELEERVPHGN"
     gene            2208..2747
                     /gene="nrdG"
                     /locus_tag="PLU15_08550"
     CDS             2208..2747
                     /gene="nrdG"
                     /locus_tag="PLU15_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOO31010.1"
                     /translation="METSVIDKLRSHASLDFHLRLAGIVDESVVDGPGVRLVVFAQGC
                     PHHCPGCHNPHTWATDGGVSVAAEEIIERLRANPVLSGVTLSGGEPFAQASEFGRLAM
                     AVKKMGFTVTTYTGYKWEHLVQSSYSGVHLLLDNTDILVDGPYVDNLREPGLKWRGSS
                     NQRIIDVHRSLGAGSVVLL"
     gene            3017..3910
                     /locus_tag="PLU15_08555"
     CDS             3017..3910
                     /locus_tag="PLU15_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOO31011.1"
                     /translation="MVKLERLANKPILRPVPEHGWERAAVFNCAAVYKDGIFHLIYRA
                     CNLPSHDRFGPYISTMGHAVSLNGVDFARDDKPVFSGEGPQEQRGVEDPRIVELDGRF
                     YMMYTAFGARFDGDYRISMASSPDLINWSRHGVVLDEPNKDASLFPEKVRGRYAMYHR
                     RHPNIWMAFSDDLVNWTDHKVIMEIIPDSWESERIGIAGPPFRVAEGWALIYHGVDSE
                     NRYRLGWALFDGDDPTKLIYRQREPILQPELEWEREGWVPNVVFSCGQAVVDDTVYVY
                     YGGADACIGVAAAKIEDFTAK"
     gene            3960..5000
                     /locus_tag="PLU15_08560"
     CDS             3960..5000
                     /locus_tag="PLU15_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO31012.1"
                     /translation="MGVTIEDVARRAGVSASTVSRTLNNKGRISAETRRRVIEAAREL
                     EYPVSADAGLSRRRTNRIGILFDRRLHSLVADPFYGLVMVGVEEWLRDEGYQVFFSTF
                     SDREADMAMISEFAAERPVDGLIMAGCDIDLDCIRKAVFMGMPLVLVDNNIVEPKVPC
                     VMTDNTAGAREAVEHLISLGHEEIGFVSGPMTHSSLYERYQGYRQAMEANGLLVRSDM
                     VIAYEDVADYDVNGGYKAAERLLKRSPRPTAIFAGNDSMAIGVMKAAQELGLVVPDDL
                     SVVGFDDIELAAHTTPALTTVRVHKRELGYHAARLLLESIDGDPDRIPYKIAVCTELI
                     VRESTCPRRLDA"
     gene            5022..5945
                     /locus_tag="PLU15_08565"
     CDS             5022..5945
                     /locus_tag="PLU15_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31013.1"
                     /translation="MKEAAVEERQPSYLARLRMEYRSSDKAGYIMVLPYVLHFLVFVA
                     YPLVFAFILVFCKWDIVSPMEFVGLRNIQRLMRDTTFWTAVTNTLKFLALHIPLQIVF
                     ALLIAVVLNGPIKLRGFFRGAFFLPFVISGAVVTILWQQLYSTDAGVFNLILTKLGAS
                     PVPWLTDPSIAIYSIAVMATWKNVGFYIVLFLAGLQNVPRELYEAASIDGANRGEQFW
                     KITMPLLRPVTLLVIMLSTIGGFGLFIEPFVMTGGGPAESTLSVVLYLYKNAFQFLKM
                     GYAATIGFALALMIFLVTLVQRKLLDVEQPY"
     gene            5962..6780
                     /locus_tag="PLU15_08570"
     CDS             5962..6780
                     /locus_tag="PLU15_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31014.1"
                     /translation="MRTFKKILGYVILTAAALTFIYPFLWMISASFKPESEIGSLSLI
                     PKNFTLAGYGAVFTQIPILKALFNSVFVSVISTACVLIFSSMVGYALARLRFIGRDTI
                     FNLVLLTMMIPGQLTLIPMYVLIVKLGWADTYKALIVPAMMSNMGIIIFRQFFMSLPQ
                     ELVDAARIDGCSDYEILFKIFWPLSKPSLVTVGITTFIGSWNEVLWPLIVIRKWDMMT
                     MPQMVTLFSVGGLAGAQVAAEMAAATMLALPVVIAYLFFQNYFIESLALSGIKG"
     gene            6919..8223
                     /locus_tag="PLU15_08575"
     CDS             6919..8223
                     /locus_tag="PLU15_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO31015.1"
                     /translation="MRTSIKSRVAAIVAALLMVSLSCGIIEAKTPVTITFWPSSNPQE
                     IEFAKIIVARWNKQNPDIQVKMEPLPASRSTEEVLLAAIAAGTTPDVCANIYPGAISQ
                     YIAGKGLYPHDKLPGFYDFVYERSPKEIVDAFTYRDGHIYQIPWKANPVMLAYNVNLL
                     RENGIDPRSLSTYSGFLKAAEKLAKDTDGDGRIDQWLYAPSTDVTWWQRFFDFYTLYI
                     AASGGKTLLDDRGRVAFEDEAAVAVFTFLRTLFDKKLVPKGTLIGDQFLGGKVATTIT
                     GPYSIPYYDQNAPEGFKYDFMPVPVPDGHKGPVVTYGDPKNIGIFANSKHPLEAWEFV
                     KFIMSRENDLLWLEITSQPPYRKDIVDDPMFAPYFKKNPMMSKFVVQGAYTRGVDDSK
                     YLIEIFDAISKQYDLCCVNGRKTPKNAVRDGARDARDILAGW"
     gene            8232..9584
                     /locus_tag="PLU15_08580"
     CDS             8232..9584
                     /locus_tag="PLU15_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO31016.1"
                     /translation="MTERTSERYVKSKRTARHVRALASCLAAGLILAGLMLAPCGGMA
                     AGGPVSIDFWCSSNPQEIEWAKAVVGKWNATRRDIVVNLQPLPASRSTEEALLAAIAA
                     KTTPDVCANIYPGVVTQFVAAGGLYRMDRFRDFIPFMEQRLPSSIMEQHRSPDGHFYQ
                     VPWKGNPIMLAYNVKMLREAGVDPASLRTYSGFLSAAKKLTRDRDGDGRVDQWMIYLN
                     IEPIWWQRFFDFYTLYVAASCGRTLIEGNRAAFNNRAGVAAMGFLAEIFKRGYAPKST
                     FPGDVFLQEKTATYITGPFAIPFWESMKPKGFEYDFVPVPVPDFYTGKEVWSYSDPKN
                     VGIFQTSKHPEEAWEFVKFMLSEENDAMFLAMTSQIPFRKNFDKVEGFASIAKKQPHL
                     AKFMVQSRYTQGADNVKPLVEIYDAIAQEYQYCAVLGQETPEEAIKIAEERVNEILSY
                     "
     gene            9636..11690
                     /locus_tag="PLU15_08585"
     CDS             9636..11690
                     /locus_tag="PLU15_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31017.1"
                     /translation="MNSARRRWALAAAACAFILMVAAFGASAAESGIEYASDAHRAVN
                     EMVNLSHLDYLRDEIVRSDGTVIPIWWVYCEPSVPGDRSSQYKYVEAASEGISCVDDV
                     ARAALAYLAEYERSGDEHALDMARDCFTFIEYMRTPEGHFYNFILESGSRNLKGATSE
                     KGVNWWTTRGMWALARGIRVFDKVDPEYAAHLEELIQPSLESIWEFVTAYELSKYGEY
                     KTLHGVKLPAWLVEDGSDASGVLVMALCELYEARPNQRTAEMAKMLADGIVGFQMGTP
                     DEFPFGAHLPWGGTITNWHGWGSHQIKALAMAGRIFGNQAWIESAEYAANSFVTHMLA
                     SIGMFAHMGPAPLSYVQLSYGAQTITTGLLELYRATGKEIYAVLGGIAGSWYMGNNVA
                     NHPMYDPATGRGWDGIDPPGPERGIGVSFNAGAESTIEAVTTMIELASVPKACEYVNL
                     ANRWKYAYRVVEAESFDNPLSGRPRKIWAQWTGENTPSGEFYVTVRSGDSFKLSFTVP
                     EDDVYIPYAVFERQPVAEGQVGMSFSIDGGEPIVVDMGGSSDTKYFVMEKLAPKVELK
                     AGKHTVTVKFVGASRAQTAALDALVFQPMIEYRHMTGPDYQNVIILRSFADKASTKGI
                     DVDIRSSAPAHEIKFTVKSYNTAGDLVSEESVIRPVDECAETVRIDPLVEAYGYTLVE
                     WR"
     gene            11763..13142
                     /locus_tag="PLU15_08590"
     CDS             11763..13142
                     /locus_tag="PLU15_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31018.1"
                     /translation="MKVRKRLAVAALLAAALLAVLSVGASAATKSVAVDVKCTTGKPV
                     PVSLTMDEVREAIGVDFPANWDSLKVVDEDGNVVPFQLDDVDMSGTVSRYDELAFLAT
                     GPVKIVVSDEAPQEKPVFEPAFRVLEIDEDNTVIESLDGLFKVKVGKHGTLDIIEYQG
                     KEHKYARDIGMIRYAGFPYSTWWYDENYDRHEEKTTFEEPLRRVKFGILEAGPARITV
                     VAHNASDLFPGLRQQMVASIYATGEIRVSNRVVSAGYADLTKLFTMCGGVMADVEDAR
                     HILPVFRWIDWADELKMTAEEYWAEQGVLTKVDGVSYIAFADSRGPKPAWWGASYLFC
                     SPERWRANVSASEGMGVAECLMDVPEVADDLEGKINMEHWHLEGEWRTGYFRWIADEI
                     FGLRQKNEIPIDIEDLEDKGPNWPLHMIPGDVFEHLNFYLLFDADGVDSAVRFLEARY
                     AELDSVEVR"
     gene            13145..14026
                     /locus_tag="PLU15_08595"
     CDS             13145..14026
                     /locus_tag="PLU15_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31019.1"
                     /translation="MALAYANEDRGAAYAAPLAKLRAVLLVVALCFGFALVSPRAPSV
                     AGAAAYTEPYFEYISSAYSIGISLDPVCIDYISIDPESQGEQRDVLAYVGKPSTLWLG
                     IGGWGTHMPTKPSRVKVLEEEGRTVLAISDIEFVGFLKTTFGEIAPVRADWVFTFYED
                     FFDMELTWRIQENVDGLQEAGWALNIDLPRYGDSEEAVRLNQDVNGYPKWIVWYGPDL
                     GVAAAHKEGSTNLRANRWYGNLPSSYVIVHTWWKPGGSGIDWGTYPGGIWRVGFFGGE
                     KQLDAAWALYGELNGID"
     gene            14056..>15214
                     /locus_tag="PLU15_08600"
     CDS             14056..>15214
                     /locus_tag="PLU15_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOO31020.1"
                     /translation="MKFITGVNYWPKNKGLAMWRDFDIREIAGDMEAIAKLGMNAVRL
                     FLRWSDFQPEPDRIDRTMMDRFEQVLEAAHKAKIGVIPTFFCGHMSGENWDVPWRGGR
                     DPYTDPEMLRAQALQVRHFAMAYRGDPRIICWDLANEQDVFVKPRDRHSGWLWTRLLA
                     SELKMNDPDHPVTLGTHISSLWEDCGFWPEDLAESLDFLCMHAYPLYSDACPERMDGV
                     RSTYFVPFCAALTRALGRGADVLFEEFGATSQMCAEDVIERYYTTSLYALLAEGVTGA
                     MGWCYGDFSVGDELPYETTPYEVGFGLVDREGCPKGAGRAMAEFCKALDCAGGGVMHK
                     AEVGAAIVVQRRYYENYDPDVTPAQNFRVLFNSYIMARRSGLNPEIVTFDDD"
BASE COUNT         3528 a         3940 c         4510 g         3236 t
ORIGIN      
        1 taaactgata tactatcgct gcaagcctat cacaacaacg gtttgagttt ggatgggcaa
       61 actgaatatc gcaaatcctg tcaggtgccc tcatgcagag ggagaatagg gaagaggcag
      121 caggctgagg ttgtgcaacc gctcctgcga agcccgcggt cccgccactg tagtcaggga
      181 gccgaacgcg aggcagcgat gccggtgtca ctttcgcaag atgaagggaa gatgcgtgca
      241 ggcgatgatc tgacagccag gagacctgcc tgacaggatg cgagccacct tccgtggaaa
      301 gagggggcgg cgcctgctcc gcatattcgc tcgccgagcg atggggagca gagaatgttg
      361 ctggcggcgt ggcccccgtt tgaccgaaaa cgggggcctg tggttttaca ggcagccagg
      421 agacgtattt agggggcgct ggtaaatgtt agaggcacaa cacgagatac atgaggcagg
      481 gcatggagag agcaagtggc ttgacctgat gaacgcgatc ggagagatca tagagaatac
      541 cagcagggag aatgcgaaca tcggaaactc tccatctgcc aagatgttgc agattgcatc
      601 ttcagccagc agagaatatt acctcgatcg tgtaatacct gacgacatcg cttgcgcgca
      661 tagaaatggc gatatccaca tacatgacct cgactactac ggcaagacat tgacttgcgt
      721 acatatcccg ttgggaagat tgctggcaga gggtttcgat accggccacg gctatgtccg
      781 cccgcccaag cggcctgcct ccgccgcggc gcttgccgca atcataatac agagttgcca
      841 aaacgacatg cacggtggcc aatcatttgc gcacttcgac accgatatgg gtccctacat
      901 ggagggcttt gatgaagacg aagcctatca gggaatggag gccttcatat acaacctcaa
      961 taccatgcac agcagagccg gggcccaagt cccgttctcc acggtcaata tcggctgcga
     1021 cacgtccgcc ggcggacgtc gcgtcacaag attgttgttg gaagcttacg agcgtgggct
     1081 tggaaggggc gaaacgcctg ttttccccaa catcatcttc agagttaaat cgggggtcaa
     1141 catgaaccct aaggacccca actacgacct tttcaagctg gcatgccgag tgtgcgccag
     1201 gcgcatgaac ccgacgttca gcttcatgga ttcctcgttc aatgcaccat acggggacga
     1261 agtggcctat atgggctgca gaacgcgggt gatggccaat cgcagaggtc aggagacaag
     1321 tgttggaagg ggcaacctct ccttcacaac catcaacctt ccccgattgg ccctaatggc
     1381 cggcggaaac gtcgaccgct ttttcaggct gctcgatacc aagctcgagc tcgtgtacag
     1441 acaattgctg cacagattcg atatccaatc gaggctcaag gttcgtgata tgccatttct
     1501 gatggggcag aagatataca ttgactcata tggacttggc gagaatgacg agattggccc
     1561 cgccatagcc aatggcacgc tttccgtagg tttcatcggg cttgcggaga cactgacagt
     1621 tctctgcggt gcccaccatg gtgaaaacga ggaagcagag cagcttggcc gggagatagt
     1681 ctccttcatg cgtcggcaga tagacggatt tgccgaggag agcaacctca actttacctt
     1741 gctggcaacc cctgcagaag ggttgtcagg ccgtttcgtg gccattgaca gggaacggtt
     1801 cgggactgtt aggaacgtga ctgataggga atattacacc aacagcttcc accttccggt
     1861 gagatatcca acgactattt cacgaaaact cgttgttgag ggtgcgttcc atggattgac
     1921 caacgcaggg cacatatcat acgtagaact ctcgtctccg cctattgaaa acggagaagc
     1981 agtggaaagc atcgtgaagg caatggccag ggcggacatg ggttacgggg gcatcaactt
     2041 tcccatagat gaatgccgca agtgcggaca tgccggggtg atcgagagct cctgcccttc
     2101 ttgcggttca ggagacatct cccgcataag aaggatcaca ggataccttg ctcccttaga
     2161 caggttcaac gacgcaaaaa agagggagct tgaggagaga gttccccatg gaaactagcg
     2221 ttattgacaa gctccgttcg cacgccagct tggatttcca cctcaggctt gccgggatag
     2281 tggacgaaag tgttgtggac gggccaggag tgaggcttgt ggtttttgca cagggatgcc
     2341 cgcaccactg cccgggctgc cacaaccctc atacatgggc gacggatggg ggcgtatcgg
     2401 ttgcggccga ggaaataatc gagaggctga gggccaaccc ggtactgtct ggagtgaccc
     2461 tcagcggggg agagccattt gctcaggctt ccgaattcgg cagattggcg atggctgtca
     2521 aaaagatggg gtttaccgtg acgacatata ccggttacaa gtgggagcat cttgtccagt
     2581 cgagctacag cggagtgcac ctgctcttgg ataatacgga tatactggta gacgggccct
     2641 atgtcgacaa cctcagagag ccgggcctca aatggcgcgg ctccagcaat cagaggatta
     2701 tagacgtgca tagatcactc ggggcgggga gcgttgtgtt gctgtaggtg agagccattt
     2761 gggcgtcagg cgcccgtttt ccacccgccc ccgcatccat cgcacatccg atgcggctgt
     2821 ttggcgccgc ggccagccaa acagcgttgt ctcaattcag ctccatatca tcattcctct
     2881 tcccaaacca caaaactttt gcgcaaagaa ggaaacctgg atgtaatgtc gaatacatat
     2941 tgcgcaatag caatgcaata tatatgtgca agtgcgcaaa tgttgcgcaa gacgtgccaa
     3001 ttacggaggt gcgccaatgg tcaagcttga gcgactcgcc aacaagccta ttctcaggcc
     3061 tgtgcctgaa cacggatggg agagggcggc ggtattcaac tgcgcagcgg tctacaaaga
     3121 cggcatcttc caccttatct accgcgcctg caatctgccc agccacgaca ggttcggacc
     3181 ctacatttcc accatggggc atgctgtgag cctcaacgga gtagatttcg ccagggacga
     3241 caaaccggtg ttttccgggg agggccccca ggagcaacgg ggggtcgagg atcccagaat
     3301 cgtggaacta gacggcaggt tctacatgat gtatacggcc tttggcgcca gattcgatgg
     3361 agactataga atatcgatgg caagttcgcc tgacctcatc aattggtcgc gccacggagt
     3421 agtcctggat gagcccaaca aagatgcctc gctctttccg gagaaggtac gtggcaggta
     3481 cgcgatgtac cacagacgcc acccgaacat ctggatggcc ttttccgatg atttggtgaa
     3541 ctggaccgat cacaaagtga tcatggagat aatacccgat tcgtgggaat cggagcgcat
     3601 aggcattgca ggtccgccct tcagagtggc tgaggggtgg gccttgatct atcatggcgt
     3661 agattctgaa aacaggtacc gcctgggctg ggcgctcttt gacggagacg atcccacaaa
     3721 gctgatttat cgacaacgcg agcccatcct ccaacctgaa ctggaatggg agagggaagg
     3781 ctgggttcct aacgtcgtct tcagctgcgg acaggcggta gtcgatgaca cagtctacgt
     3841 ctactatggc ggcgcagacg cctgcatcgg cgttgctgca gcaaagatcg aagactttac
     3901 tgcaaaatag gggcaaaaaa cgtgcgcaag ttgcgcaaaa cggaacggag agtggcgcca
     3961 tgggagttac gatagaagac gtcgcaagga gggctggcgt ttcagcgtcg accgtgtctc
     4021 ggaccctcaa caacaagggc aggataagtg ctgagacgag gcggagagtg atagaagcag
     4081 cccgcgaact ggaatacccg gtgagtgcgg atgcgggcct ctcccgccga cggaccaatc
     4141 gcataggaat actcttcgac cgacgccttc attcgctcgt cgcagaccca ttctatggcc
     4201 tggtaatggt cggggtcgag gaatggctgc gcgacgaggg ttatcaggta ttcttttcca
     4261 cattctcaga tcgtgaggcc gacatggcca tgatctcaga gtttgccgcg gagcgccctg
     4321 tggacgggct gatcatggca ggctgcgaca ttgacctcga ttgcatcagg aaagcggtat
     4381 tcatgggaat gcctctggtg ctggttgaca acaacatagt tgagcccaag gtcccctgcg
     4441 tgatgactga caatacagcg ggggcgcggg aagctgtgga acacctgatc agtctgggcc
     4501 acgaagagat cggattcgtc tcaggcccca tgacccatag ctccctttat gaacgttatc
     4561 agggctatag gcaggccatg gaggcaaatg gccttttggt caggtctgat atggtcattg
     4621 cgtatgaaga tgtggccgat tacgacgtca atggaggtta caaggccgcc gagcgccttt
     4681 tgaagaggtc gcccaggccg actgcgatat tcgcaggcaa tgattccatg gcgatcggag
     4741 tcatgaaggc cgctcaggag ttgggcctcg ttgttccgga cgatttgtcg gtggtcggat
     4801 tcgatgacat cgaattggcg gctcatacaa cccccgccct cacaaccgtg cgagtgcaca
     4861 agagggagct tgggtatcat gcagcccgcc ttctcctaga gtccatagac ggggatccag
     4921 accgcatccc ttacaagatc gcggtctgca ccgaattgat tgtccgcgaa tcaacatgcc
     4981 ccagacgtct cgatgcatag ttttgtgtgg gggtgactta agtgaaggag gctgcggtcg
     5041 aggagaggca gccatcatac cttgcgcggc tgcgcatgga gtaccggtcg agcgacaaag
     5101 caggatacat aatggtgctt ccttatgtat tgcattttct cgtctttgtt gcatatcccc
     5161 tcgtatttgc gttcatactc gtgttttgca agtgggacat cgtatcccca atggaattcg
     5221 tcggactgcg taacattcaa agactgatgc gtgatacgac gttctggact gcagtcacga
     5281 acaccctgaa gttcttggct ttacacattc cgcttcagat agtctttgca ctcttgatcg
     5341 cagtggtgct caacggcccg ataaagctga gaggtttctt taggggcgcg ttcttcctgc
     5401 cattcgttat ctccggcgca gttgtcacaa ttctttggca gcagctttac tccaccgatg
     5461 caggagtctt caacttgatc ctgaccaagc taggcgcatc tcctgtgcca tggctcactg
     5521 acccgtctat cgccatctac tctattgcag tcatggccac gtggaaaaac gtcggattct
     5581 acatagtgct gtttctggca gggctccaga atgtgccgcg cgagctttac gaggccgcga
     5641 gcatagatgg ggccaaccgg ggagaacagt tctggaagat cacgatgccg ctgctcaggc
     5701 cggttacgct gcttgtgatc atgctctcta cgataggcgg gtttggtctt ttcatcgagc
     5761 cattcgtgat gaccggcggt gggccggcag agagcactct atccgtggtt ctgtatttat
     5821 acaagaacgc gttccagttt ctaaagatgg gttatgcggc taccataggg tttgcgttgg
     5881 cgcttatgat ctttttggtc acgttagtcc agagaaagct gctcgatgtc gagcagccat
     5941 attagagcgg aggcggtagc gatgcgaaca ttcaagaaga tattgggcta tgtaatcctg
     6001 accgcggccg ctctcacatt catataccca ttcctctgga tgatctcggc atcgtttaaa
     6061 cccgaaagcg agatcggcag cttgagcttg attccgaaga acttcacgct cgcaggctat
     6121 ggggcggtgt ttacccagat cccaatcctc aaggccttgt tcaactcggt atttgtctcg
     6181 gttatatcga ccgcctgcgt cctcatattc agctccatgg tgggctacgc tctggcccgg
     6241 ctcaggttta taggacgtga caccatcttc aacctcgtgc ttctgacgat gatgatcccg
     6301 ggccagctga cgctaatacc catgtacgtg ctgattgtga aattaggttg ggccgatacc
     6361 tacaaggcat tgatagttcc ggccatgatg agcaatatgg gaatcatcat tttcaggcag
     6421 ttcttcatgt cgcttcccca ggagctagtg gatgcggcga ggatcgacgg gtgctccgac
     6481 tacgagatac ttttcaagat cttctggccc ctttccaagc catcactggt tacggtgggc
     6541 atcaccacct tcatcggctc atggaacgaa gtgctatggc cgctcattgt gatcaggaaa
     6601 tgggacatga tgaccatgcc gcagatggtg actttgttca gcgtcggggg actggcggga
     6661 gcgcaggtgg ctgcggagat ggctgctgca accatgttgg cgttgcctgt tgtaattgct
     6721 taccttttct tccagaacta cttcatagaa agcctggcgc tatcgggcat caagggatag
     6781 caacgaaggc tagagccttc tcagagctgg tggttggaac ccgaaacaaa aacccttgtg
     6841 gtacgcactt tactcctcgc gcaggagcgc gggttcatat gaacaaacaa gcgagattac
     6901 tgaaatggag ggacggcaat gcgaaccagc atcaaatcgc gtgtagcggc catcgtggct
     6961 gctctactga tggtgtcgtt gtcatgcggg atcatcgagg cgaagacgcc ggtcaccatc
     7021 acattttggc cgtcgtctaa cccgcaggag atcgagtttg ccaagatcat agtggcaaga
     7081 tggaataagc agaatccgga catccaggtg aagatggagc cgcttccggc atccaggtct
     7141 accgaagaag tgctgcttgc ggcgatagcg gcaggcacga ctccggacgt gtgcgcgaac
     7201 atctacccgg gcgcgatatc ccagtacatc gcgggcaagg gcctttatcc ccacgacaag
     7261 ttgcctgggt tctacgattt cgtctacgaa agaagcccga aggaaatagt ggatgccttc
     7321 acttacagag acggccatat ataccagatt ccgtggaagg caaaccctgt catgttggcc
     7381 tacaacgtta acttgctccg ggagaacggc attgacccca gatcgctctc tacctacagc
     7441 ggtttcctga aggcagccga gaagctcgcg aaggataccg acggagacgg ccgcatcgac
     7501 cagtggctct acgctcctag cacggatgtt acgtggtggc agcggttctt cgacttctac
     7561 accctctaca tagccgcgag cggcggcaag acactccttg acgaccgcgg cagagtggca
     7621 tttgaagacg aagctgcagt tgcagtcttc acatttctga gaaccctatt tgacaagaaa
     7681 ctcgttccca agggaacgct tatcggcgac caattcctag gcggcaaggt agccaccact
     7741 ataacgggac cgtactccat accttactac gaccagaatg ctccggaggg cttcaagtat
     7801 gacttcatgc ctgtgccagt tccagacggg cacaagggcc ctgtagtgac ctacggagac
     7861 cctaaaaaca tagggatctt cgcaaattcg aagcatccgc tcgaagcgtg ggagttcgta
     7921 aaattcatca tgtcccgcga gaacgacctt ctctggctgg agatcacatc tcagcccccg
     7981 tatcgcaagg atatcgtgga cgatcccatg ttcgcgccct atttcaagaa aaatccgatg
     8041 atgtccaaat tcgtcgttca gggcgcctac acccggggcg tggatgacag caagtatctg
     8101 attgagatct tcgacgccat atccaagcag tatgaccttt gctgtgtgaa cgggcgcaag
     8161 actccgaaga atgcagttcg cgacggcgcg cgggacgctc gcgatattct ggcagggtgg
     8221 tagtgcacgt gatgactgag agaacttcag aaagatacgt caaaagcaag cggacggccc
     8281 ggcatgtccg ggccctcgcc agctgtctgg ctgcagggct gatcctggcc ggattgatgc
     8341 tggcgccttg tggcgggatg gcggcgggtg gccctgtttc catcgacttt tggtgttcat
     8401 ctaatcctca ggagatcgaa tgggccaagg ctgttgtggg taaatggaat gcgaccaggc
     8461 gcgatatcgt ggtaaacctt cagccattgc ccgcttcgcg ctcgacagag gaggcattgc
     8521 tcgccgcaat agcggcaaag actaccccgg atgtgtgcgc aaacatctac cccggagtgg
     8581 tcacgcaatt cgtggcggca ggcggattgt atcggatgga cagattccgt gatttcatcc
     8641 cattcatgga gcaacggttg ccttcctcca ttatggagca gcatagatcg cctgacggcc
     8701 acttctacca agtcccgtgg aagggcaacc ccataatgct cgcctacaac gtgaaaatgc
     8761 ttcgcgaagc aggcgtcgat cccgcatcgc tcaggacgta cagcggtttc ctgtcagcgg
     8821 ccaaaaagct cacacgggac agagacggag acggcagagt cgaccagtgg atgatctacc
     8881 tcaacataga gccaatctgg tggcagaggt tcttcgactt ctacaccttg tatgtagccg
     8941 ccagttgtgg gcgaacgctg attgagggga accgggcggc cttcaacaac agggcgggag
     9001 ttgcggctat ggggtttctt gctgagatct tcaagcgggg ctacgccccg aaatcgacct
     9061 tcccaggtga cgtgttcctg caagaaaaga cggctacata cataaccggc ccgtttgcta
     9121 tacccttctg ggaatcgatg aagcctaaag ggtttgaata cgacttcgtg cctgttcctg
     9181 tccccgactt ttacacgggc aaggaagttt ggagctatag tgatccgaag aacgtaggca
     9241 tcttccagac cagcaagcat cctgaagaag cgtgggagtt tgtcaagttc atgctgagcg
     9301 aggagaacga cgcgatgttt ctggcgatga cgagccagat cccgttccgc aagaactttg
     9361 acaaggtaga aggtttcgct tcaatagcga agaagcagcc gcacctcgcc aagttcatgg
     9421 tccaaagcag gtatacccaa ggggcggaca acgtgaagcc tttggtggag atatacgacg
     9481 cgatcgccca ggagtatcag tactgtgccg ttctcgggca agagactccc gaggaagcca
     9541 taaagatcgc cgaggagagg gtaaacgaga tattgagcta ctgagcagcc aagctgttgc
     9601 atcagtcggg ctcagcagca tagggggata tgaacatgaa ttcagctagg cgacgctggg
     9661 cactggcggc cgcggcctgc gccttcatct tgatggtggc ggcgttcggg gccagcgctg
     9721 ccgaaagcgg tatagagtac gcaagcgatg cccacagggc tgtgaacgag atggtcaacc
     9781 tgtcgcattt ggactacttg cgcgacgaga tagttcgctc cgacgggacg gtcataccca
     9841 tctggtgggt atactgcgag ccttcggttc cgggcgacag gagctctcaa tacaagtacg
     9901 tggaagcggc gagcgagggc ataagctgcg tagatgacgt ggccagggcg gcccttgcat
     9961 accttgccga atacgagcgt agtggagatg aacacgccct cgacatggca agggattgct
    10021 tcacgtttat cgagtatatg cgcaccccgg agggccactt ctacaacttc atcttggaat
    10081 cgggctcccg taacctgaag ggcgctacga gcgagaaggg agtaaactgg tggaccacca
    10141 ggggcatgtg ggctctggcc cggggcatca gggtattcga caaggtcgat cctgaatacg
    10201 cggcccacct tgaggagctg atccagccgt ccttggagtc gatttgggag tttgtcaccg
    10261 cttatgagct ctccaaatac ggggagtaca agacccttca tggcgtgaag cttccggcat
    10321 ggctagtgga ggacgggtct gatgcctcag gagtcctcgt gatggccctt tgcgagctgt
    10381 atgaggcgag gcctaaccag aggactgccg agatggcaaa gatgctcgcc gacggaatag
    10441 tcggttttca gatgggtacc cccgatgagt ttcctttcgg cgctcacctt ccatggggtg
    10501 gaacgatcac caactggcat ggctggggga gccatcagat aaaggcccta gccatggctg
    10561 gaagaatctt cggcaaccag gcgtggatcg aatccgcgga atatgcggcc aacagcttcg
    10621 tcacgcatat gctggcgtcg atagggatgt tcgcgcacat gggcccggca ccgctctcct
    10681 acgttcagct gtcgtatgga gctcagacca tcaccacggg cttactcgag ctttaccgcg
    10741 caacgggcaa ggagatctac gcagtgcttg gcgggatcgc aggatcgtgg tacatgggga
    10801 acaacgtggc gaaccacccg atgtatgatc cggccacagg gcgcggatgg gacgggatag
    10861 acccgcctgg gcccgaacgc gggataggcg tgagcttcaa tgccggagcc gaatcgacca
    10921 tcgaggctgt aacgacgatg atagagcttg cttcagtgcc gaaggcatgt gaatacgtaa
    10981 accttgccaa cagatggaaa tatgcatacc gagtggtcga ggcggagtcc ttcgataacc
    11041 cgctatcggg ccgccctcga aagatctggg cgcagtggac aggggagaac acgcccagcg
    11101 gagagtttta cgtgactgta agatccggtg acagcttcaa gctgagcttc acggtccccg
    11161 aagacgacgt ctacatccca tacgcggtct ttgaacgcca acctgttgcc gaggggcaag
    11221 tcggaatgag cttttctata gacggcggag aacccatcgt agtggacatg ggcggatctt
    11281 ctgatacaaa gtatttcgtg atggaaaaac tcgcgccgaa agtcgagctt aaagccggca
    11341 agcacactgt gaccgtgaag tttgtcggtg ctagccgggc gcagaccgca gcgctggacg
    11401 ccctcgtttt ccagccgatg atcgagtatc gccacatgac agggccggat tatcagaacg
    11461 ttatcatcct ccggagcttt gccgacaagg cctccaccaa aggcatcgat gtggacatac
    11521 gttcgagcgc gccggcgcat gagatcaagt ttaccgtgaa gagctacaac acagccggtg
    11581 atctcgtatc agaggaaagc gtgatacgtc cggtggatga atgtgcggag acggtcagga
    11641 tagatccctt agtggaagct tacgggtaca cattggtcga gtggcgttga gacttgcagt
    11701 ccagcgccag agcacaatcg gcctaagggc cgacccaaaa aaatcgagga ggaacgtgaa
    11761 agatgaaggt tagaaagaga ctcgccgttg ctgctcttct tgcggcagca ttgctcgccg
    11821 tattgagtgt gggggcatcg gcggcaacca agtccgttgc ggtcgacgtg aagtgcacga
    11881 caggcaaacc cgttcccgtg tcgctcacga tggatgaagt ccgtgaggcc atcggagtag
    11941 acttcccggc caactgggat tctctaaagg tagtagatga agacggaaac gtcgttcctt
    12001 tccagctgga tgacgtggac atgtcgggaa cagtctcccg ctacgatgag cttgccttcc
    12061 tggcgaccgg cccggtcaag atcgtcgtgt cagatgaggc gccacaagag aagccagtct
    12121 ttgagccggc atttcgcgtt ctcgagatcg acgaggacaa cacggtcatc gaaagccttg
    12181 acggtctttt caaggtcaaa gtaggtaagc acgggaccct cgacataatc gagtaccagg
    12241 gcaaagaaca caagtatgcc agggatatcg ggatgatcag gtacgccggg ttcccctaca
    12301 gcacctggtg gtatgatgag aactacgaca gacacgagga gaagacgacg tttgaggagc
    12361 ctctccgccg cgtcaagttc gggattctcg aggcagggcc cgccagaatt acggtggttg
    12421 cacacaacgc atctgatctc ttccccggct tgcgccagca gatggtggcg tcgatatatg
    12481 ccacgggaga aatcagggtg tcgaaccgcg tggtttctgc tggatatgcc gacctcacaa
    12541 agctctttac tatgtgcgga ggcgtcatgg ccgatgttga ggacgcaagg cacatactgc
    12601 ccgtattccg ctggatcgac tgggcggatg agctgaagat gactgccgag gaatactggg
    12661 ccgagcaggg agtgctcaca aaagtggacg gtgtgtccta catagccttt gcagattccc
    12721 gcggaccgaa gcctgcgtgg tggggtgctt cctatctctt ctgcagcccc gaaagatggc
    12781 gcgccaacgt ctcagccagt gaaggcatgg gcgtcgcaga atgcttgatg gatgtgcccg
    12841 aggttgcaga tgatctcgag ggcaagatca acatggagca ctggcacctc gaaggcgaat
    12901 ggaggaccgg ctatttccgg tggatcgccg acgagatctt cggcctcagg cagaagaatg
    12961 aaatccctat agacatagag gacctcgaag acaagggacc caactggcca ctgcatatga
    13021 tcccaggcga tgtcttcgag cacctcaact tctatctgct atttgatgca gatggtgttg
    13081 attccgctgt caggttcctc gaggcgagat acgccgaact cgacagcgta gaggttcgat
    13141 agcgatggca ttggcatatg cgaatgagga caggggcgcg gcgtatgccg cgcccttggc
    13201 caagcttaga gcagtattgc ttgtcgtcgc actctgcttt gggttcgctc ttgtctcgcc
    13261 ccgggcgcca tccgttgccg gtgccgcggc ctacaccgaa ccatatttcg aatacatcag
    13321 ttccgcatac tccataggca tttcgctcga tcccgtctgc atcgactata tttccatcga
    13381 ccctgagagc cagggagaac aacgtgatgt tctcgcgtat gtgggcaagc cgtccactct
    13441 gtggctgggg atcgggggat ggggcactca tatgccgaca aaaccgtccc gagtgaaagt
    13501 tctggaagaa gaaggcagga ccgtgctggc tatctcagac atcgaattcg tgggctttct
    13561 caagacgaca ttcggggaga tcgctcctgt ccgggcggat tgggtattca cattctatga
    13621 ggatttcttc gacatggaac tcacctggcg cattcaggag aatgtcgatg gattgcagga
    13681 ggcgggctgg gcacttaaca tcgatttgcc acggtatggc gattctgagg aggccgtacg
    13741 gctcaatcag gatgtcaatg gttaccccaa atggatagtg tggtatgggc ccgacctcgg
    13801 tgtggcagca gctcacaagg aaggttcaac gaacctcaga gcgaaccggt ggtatggaaa
    13861 tctgccttcg agttatgtga tagtccatac ctggtggaag ccggggggat cgggcatcga
    13921 ctggggaacg tacccaggcg gcatttggag ggtgggtttc ttcggaggcg aaaaacagct
    13981 cgatgcagct tgggctcttt atggagaact caacggcata gattgagata gattgcagca
    14041 tggagggggc accatatgaa gttcataaca ggcgtcaact actggcctaa aaacaagggg
    14101 cttgcgatgt ggagggactt cgacatccgt gagatcgcag gcgacatgga agccatcgcg
    14161 aaactgggca tgaacgcggt gagactcttt ttgaggtgga gcgattttca gcccgagccg
    14221 gatcgaattg accggactat gatggacagg ttcgagcagg tgctcgaggc tgcccacaag
    14281 gcgaaaatag gagtgatccc aacgttcttc tgcggccaca tgagcggaga aaactgggac
    14341 gtgccttggc gcggcgggcg cgacccttac accgatccgg agatgctcag ggcacaagcg
    14401 cttcaagtgc gccatttcgc catggcatac aggggcgatc ccaggatcat ctgctgggac
    14461 ctcgccaacg agcaggatgt gttcgtaaag ccccgggata ggcactccgg ttggctatgg
    14521 acccggcttc tcgcatcaga gctgaagatg aatgaccccg accatccggt cacccttgga
    14581 acccacatat cgagcctgtg ggaggattgc ggtttttggc cggaggacct agcagaatcc
    14641 cttgacttcc tctgcatgca tgcgtacccg ctctactcgg atgcgtgccc tgagcggatg
    14701 gatggcgtaa gaagcaccta ttttgtcccg ttttgcgcgg ccctcactcg tgccctaggg
    14761 cgcggtgctg acgtgctttt cgaggagttt ggggcgacaa gccagatgtg cgcagaagac
    14821 gtgattgagc gatactatac gaccagcctc tatgcgctcc ttgccgaggg agtgacaggg
    14881 gcgatggggt ggtgctacgg ggatttttca gtcggcgacg agcttcccta cgagacgacc
    14941 ccttacgaag tggggttcgg cttggtagac agagaaggat gccccaaggg cgcaggacgc
    15001 gccatggccg agttctgcaa ggcgcttgat tgcgcgggtg gcggggtaat gcacaaggct
    15061 gaggttggag ccgcaatcgt agtgcaaagg cgttactacg agaactatga ccccgatgtt
    15121 accccggccc aaaacttcag ggtgctcttt aattcctaca taatggccag acggagcggg
    15181 ctcaacccgg agattgtgac attcgacgat gacc
//
LOCUS       DAOSYL010000056        15089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3318_length_15089_cov_7.491619, whole genome shotgun sequence.
ACCESSION   DAOSYL010000056 DAOSYL010000000
VERSION     DAOSYL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15089
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_15089_cov_7.491619"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..352
                     /gene="secG"
                     /locus_tag="PLU15_08605"
     CDS             134..352
                     /gene="secG"
                     /locus_tag="PLU15_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOO31021.1"
                     /translation="METLLIVLEFILSILLIVVVILQQSKGEGLGAIGGGGQLFFGKS
                     KAAEAFLDKATTWLAIGFLIVSFLFVFV"
     gene            475..1026
                     /locus_tag="PLU15_08610"
     CDS             475..1026
                     /locus_tag="PLU15_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO31022.1"
                     /translation="MTRDEALCVVKANVRNGNLVKHMIACGACMKALASRFGEDEAKW
                     ELAGLLHDIDYDSAGSDPSRHSLEGAEMLDGMGVDHDVVYAVKVHNDYHGLPRKAPMD
                     RALYAVDPLTGLIVASALIHPSRKLAAIDTDFVINRYREKGFAKGARRDQIACCDELG
                     MDLREFIGVCLGAMCDCADELGL"
     gene            1063..1530
                     /gene="smpB"
                     /locus_tag="PLU15_08615"
     CDS             1063..1530
                     /gene="smpB"
                     /locus_tag="PLU15_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOO31023.1"
                     /translation="MAEKGSSVKLVATNRKARHEYFIEETYEAGIALTGTEIQSVRQG
                     RVSLADAYAEVRNGEVFLINAHIAEYDFGNRFNHDPRRPRKLLLHKKEIMRLDSRVRE
                     RGYTIVPLKMYIVRGYAKVEIALAKGKKTWDKRRDIAERDARREMDRAAKARR"
     gene            1633..1983
                     /gene="ssrA"
                     /locus_tag="PLU15_08620"
     tmRNA           1633..1983
                     /gene="ssrA"
                     /locus_tag="PLU15_08620"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2301..2912
                     /gene="prxU"
                     /locus_tag="PLU15_08625"
     CDS             2301..2912
                     /gene="prxU"
                     /locus_tag="PLU15_08625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259907.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:2442..2444,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOO31024.1"
                     /translation="MQARIGKPAPDFETGAFVAGEFKKVKLSNYRGKWVVLCFYPGDF
                     TFVUPTELAAVAVKYPELQSMGAEVLAISVDSKFSHKIWQETELSKMVAGGVPYPILA
                     DQGGKIGTLYGVYDEEAGVNVRGRFIIDPDGVIQAMEVLTPPVGRNVSELVRQVNAYQ
                     HHRATGDVMPSGWEPGKPTLKPGPSLVGRVWEVWKPEQAFGKR"
     gene            4361..5311
                     /gene="rnhC"
                     /locus_tag="PLU15_08630"
     CDS             4361..5311
                     /gene="rnhC"
                     /locus_tag="PLU15_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOO31025.1"
                     /translation="MKEERVTEASISSLRRLAKERGWTMEERDIEHGRQARVSDGENK
                     ATISFYTTGKALIQGRPSSLTEELDSWWAGSHLNRSAYVPPPNSGHGSDANKPSYSGR
                     ARARGVARIGIDESGKGDYFGPLVAAGVYVDAQTETELLSSGVQDSKRLTDRRIMELS
                     GLIVEICPHSVVAIGPARYNGLYKKMGNVNSMLAWAHARCLENLLETVSCELAVSDQF
                     GDESYLKNALMHRGRQITLEQRPRAEEDVAVAAASILARAEFVRRLESLSQQAGVELP
                     KGASDPRIVEVASEIIAREGRDALANYAKVHFRTTDALGV"
     gene            5372..5878
                     /locus_tag="PLU15_08635"
     CDS             5372..5878
                     /locus_tag="PLU15_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31026.1"
                     /translation="MTEYQRPQQKRSRIVGTLLIAIGCFLLAAQFLDTRLVSLLVLPL
                     LGFIFLAWGTVGANKAMAIPGGILLGIGTGVIMSEEVFWYLGQPASTGVFLVCFALGW
                     LVAYIVSYKTGGRKTSWLLVLGVVLAAVGIPLILAQTAPEIVKWIATYWPALLIAAGI
                     MLIVRGVK"
     gene            5964..7142
                     /locus_tag="PLU15_08640"
     CDS             5964..7142
                     /locus_tag="PLU15_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOO31027.1"
                     /translation="MKPRIIKEGVTWVGAVDWDRRLFDCLVPIPDGTSYNSYLVQGKD
                     KTVLLDTVDPSMVGQLVDHLSDISTIDYVIAHHAEQDHSGSIPKILEMYPGAKVVCTP
                     KCVEMLVDLLHVDRHRIISVEDGDMIDLGGRTLRFVHAPWVHWPETMFTYLEEDKILF
                     TCDFLGSHLATSQLYSKNEPAEEAAAKRYFAEIMMPFGSMIVKNLDKISAYDIEIVAP
                     SHGPIHDNPEFIIERYKEWTSGPMKNEVCIAYVSMHGSTAAMVDALVEELVERGVGVR
                     RFELTGSDTGAIAMSLVDAATVVIATPTVLAGAHPAAAYVAFLANALRPRTRYLSVLT
                     SYSWGGKAVEQIAGMTTNLRAEVLEPVICKGLPREDDFAAIARLAELIAEKHREIGIL
                     "
     gene            7201..7755
                     /locus_tag="PLU15_08645"
     CDS             7201..7755
                     /locus_tag="PLU15_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOO31028.1"
                     /translation="MANERISVVQGDITLERVDAIVNAANSSLLGGGGVDGAIHRAAG
                     PKLLTECRRIGGCDTGEAVVTGGYRLPAKWVIHTVGPVWHGGGLGEAELLTNCYRNAL
                     RLAVENGARTVAFPSISTGAYRYPVDEASRIAIRTVSEFLSVDDTIEDVRFVCFDTHT
                     YSAYLRALAEHLGTQGLPRNSASP"
     gene            complement(8239..8514)
                     /locus_tag="PLU15_08650"
     CDS             complement(8239..8514)
                     /locus_tag="PLU15_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31029.1"
                     /translation="MRVTGLILGLIGSIWGMFVPVDDLRWLVVLISIAGLIAACCAMA
                     YPKWAGIIMLIAAVLGWIFGKGDFGWPGAILLIGGIFALSGQGELES"
     gene            9126..10169
                     /locus_tag="PLU15_08655"
     CDS             9126..10169
                     /locus_tag="PLU15_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO31030.1"
                     /translation="MRPTLQDMADILNLSKSTVSRALSGDPRVAQATRARVESLAERI
                     GYTPNPIARGLATRRTNTIGLAVPCAPRSLSDPFYLEFLGHAGNAAMKCGYSLLLSAA
                     EEDGADTHNAHVRLASSARIDGMILTEPKIKDERIAALKKAGIPFVFLGMTSDPDVSW
                     VSCDNRLGAAEAVAWLTGLGHTRIACLTGPADQTASDARYRGYCEALDEAGIPLDLNI
                     VASGDFTQAGGHAAMREILERGKTVSAVFASNDVMALGAMCALRESGLSVPEDVSLVG
                     FDGIAFAQYSTPPLATVQQPIDELGNASVEVLIKLMCGDNGGPIHRVMPARFRPAGSV
                     AAPGRAFVLESPD"
     gene            10317..11498
                     /locus_tag="PLU15_08660"
     CDS             10317..11498
                     /locus_tag="PLU15_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO31031.1"
                     /translation="MKRKMIVLLILALAICSLPGATAEKKLTVISGWSGPEMDAFMPV
                     LKAFEKETGIRVDYQIYRAEDLAVLLPAQFNAKTTPGDVIYMWSWFITKHAKDGHVLE
                     MTGSLKDADFIPGALDAVKVDNKLWGGVYTGKVKPGFWYRKSFFKKHGLSEPKTWDEF
                     VALCAKLQKISGVKAPIASGNGVGWPLSDITEHFIATYAGPQVTYGLIDKKVKWTDPV
                     VKSVFTDRIVPLLKAGYFSEPIEWTMALDLWWRGDYGLYFMGSWITGMVDNPDDLGVF
                     SLPGCEGIVFAPDYAFVPAYTKYPAEARKLLAFLSTKGQEIQVSTGGHIATYKVPLNA
                     YPPVDRGVASLLKGVVALPDLDDTIGGEWQAAFWDQLKLLWVSPGRVNEVLENLQRKA
                     N"
     gene            11595..12467
                     /locus_tag="PLU15_08665"
     CDS             11595..12467
                     /locus_tag="PLU15_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31032.1"
                     /translation="MGQTRGRTLFFIPALALLALFVVYPVANTIWLSFFTPEGEFAFF
                     KNYADVLSQPEIFDPRGFERGFPYGALIHNVIWIVLHLPLTVILGLVFAVILRDIKGG
                     AIVKSIVFLGMVTPMVVGGIILRFLFDGNVGIIPSVLALFGFAPRTLTAYPDTALFSL
                     IAGSLWLWTPFSMVLYSAGLETIPDFVYEAAVIDGASPWRIFFNITVPLLKPVTGVVV
                     TMTFLWVLKIFDIVFVATMGGPGGASNVLALQMYMYAFREFNPNAAAVVATFLMIVAL
                     VVSIPTFKAAGGEE"
     gene            12464..13309
                     /locus_tag="PLU15_08670"
     CDS             12464..13309
                     /locus_tag="PLU15_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31033.1"
                     /translation="MMRTLGCSSGHKVITYVAAGLIALIWLIPFLGVFMASIRPLSEI
                     NRGWWRFDSFTPTFKNYVGAWNHPAAPISEGMLNSLKVAIPATIIPVFVASIAAYGFA
                     RFKFRGRDQLFILVVALMTLPQQMIAVPMFQMMNKLGAVDSYSGLILVHSAWGIPWAL
                     YFMRNYFTTLPIEVEEAAKVDGASDWRIFFQIIMPMALPAIASAAVLQFMWVWSDFFL
                     ALILIYSPAKLLATQRVPLLRGIYHVDWGILAAGSIIVMAVPIIVFAVMQRYYIRGMV
                     GWVSK"
     gene            13345..14601
                     /locus_tag="PLU15_08675"
     CDS             13345..14601
                     /locus_tag="PLU15_08675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO31034.1"
                     /translation="MFRKVDFLEKSLDDHAPYAGEDRIAEIRRLAEDLGSLRVLHVNS
                     TSYGGGVAELLYTIVPLMRDAGLDAEWHVIDGAPDQFFDITKKIHNSLQGANTPLSDG
                     EWRLYEDINRKLAEAFPRGPWDIVVIHDPQPLAVLTFLDSLSESDRPVVGRWFWRCHI
                     DLSTPLEETWQKLWPYVNAYDGAIVTSRQYARDEMTLPVAEITPSTDPTSPKNLPIPE
                     GEARKMMGSFGVDPARPIIVQVSRFDPWKDPFGVVEAYRMLKGKWPELQLAMVGSIAA
                     DDPEGIGILAHLKEEAAGDRNIHVLSNEDGVGAREVAAFQQCADVVIQKSIREGFGLT
                     ITEAMWKRRPVVAGNATGCKIQITDGVDGFIVGTTQQCAERIDEILSDPDLGARLGDA
                     ARETVRRRFLSTQHVANYLNLFAGCQ"
BASE COUNT         3485 a         3419 c         4382 g         3803 t
ORIGIN      
        1 gggattagcc aatggtctct tagctgttct ttcctacagt aggttgacgc cgtcccgaag
       61 gagcggtacc gttgtaaagc aggacggtgt atgatagaat cagttggagt cggagcgatg
      121 ggaggttgct gcgttggaga cgcttttgat tgttcttgag tttatcttgt caatactcct
      181 tattgtggtc gttattctcc agcagagcaa gggagaaggg cttggggcca taggcggtgg
      241 agggcagctg tttttcggca aaagcaaggc tgcggaggcc ttcctcgaca aagcaaccac
      301 ctggcttgca atagggtttc tgatcgtttc atttctgttt gtttttgttt aaccctggca
      361 atcagggagg caatgttata ggctgaaaca gtagcctcgc acttgagtcc gggtgcgagg
      421 ttttcccgta tttacagggg ccaggccgcg gggacagtga tgggagtgac ggatttgaca
      481 agagatgaag cgttgtgcgt ggtaaaagca aatgtgcgaa atggcaatct ggttaagcac
      541 atgatagcct gtggtgcgtg catgaaggcg cttgcttcga ggttcggcga agacgaggcc
      601 aaatgggagc tggcggggct tctccacgac atcgactatg atagtgccgg gtcagatcca
      661 tcacgtcatt cgttggaagg agcggagatg ctagatggaa tgggcgttga tcatgatgtg
      721 gtctatgcag tgaaggtcca caacgactac cacggactgc ccaggaaagc accgatggat
      781 cgcgccctgt acgcagttga tccgctcact ggcttgattg ttgcatctgc gctgattcat
      841 ccttccagga aactggcggc catagatact gatttcgtta tcaaccgtta tcgtgagaaa
      901 ggatttgcca aaggtgcgcg gcgggaccag attgcctgtt gcgatgagct gggaatggat
      961 ttgcgggaat tcataggagt ctgcctgggc gcaatgtgcg attgcgcaga tgagttggga
     1021 ctgtaatgga agtcttccgt atactgaggg gaggtgtgtc cgctggctga aaagggatca
     1081 tctgtgaagc tggttgcaac taacagaaaa gcgaggcacg agtactttat cgaggagacg
     1141 tatgaagccg ggatcgcgct tacaggaacc gagattcaga gcgttaggca gggaagggtc
     1201 agcctggcgg atgcgtacgc tgaggtgcgc aatggtgaag tgttcttgat caatgcgcac
     1261 attgccgaat acgactttgg gaacaggttc aaccatgatc cgcgtagacc tcgcaagctt
     1321 cttctccata agaaggagat aatgcggctc gactctcgcg ttcgcgagcg gggatacacc
     1381 attgttccgc tgaagatgta tattgttcgt ggctatgcca aggtcgagat cgcgctcgca
     1441 aaagggaaga agacctggga caagcgacgg gatattgcag agcgagatgc tagacgtgaa
     1501 atggataggg ctgccaaggc gagacgttaa aggatgggtg tgaccaagat caatggaagt
     1561 gtatgccatt gcacatatgc tgatccattt gatcgagttg gcggccgcgt ggtataatat
     1621 tgtgtaagat atgggggcgt tacggtttcg acggagagtg ctgtgagagg cgcagcgagc
     1681 cgagatccca cctactcgtt aatcggtgga agcacgataa gtgccaacga ttacgcttac
     1741 gctgcttaat taactaagta gccgtccgac ccgatcttct cccgaaagct cggctagggc
     1801 gtcatctaag tcgggatgct tgcgtgtcaa ttctcggagg cacgcactaa gaatatcgag
     1861 atagtccccg ggcagcgtgc ccgtgcgcgg ctcgtggggc gaaaacaagt atgcgggcta
     1921 cgctcgtaga agtgcctcaa gtggcctttt cggacccggg ttcgactccc ggcgcctcca
     1981 ccatttttat gcgcttttcg gcgctatgaa tgaggttcgc agtcttatcc catcagggag
     2041 ggctgcgaat tgttgcttta tatggggctg agtcgatctt gcgacaatac gggatgagcg
     2101 aatcgattct gcttcgggaa aaatggcctt atagacgatt gcgtgatgca agcggatcac
     2161 gagtgggaat ggctagattc ttggcttgtg gatgtcggca ctgcttttca gaatatgttg
     2221 cggaaatcac aatttggata atgtggagga ataagcgtgc atatggagaa agtaccttcg
     2281 ttaaggaggt gtattgttat atgcaggctc gaataggtaa accagctccg gatttcgaga
     2341 caggggcatt tgttgctggt gagttcaaaa aagtcaaact atcaaactat cgtggtaagt
     2401 gggttgttct atgtttttat ccgggagatt ttacatttgt ttgacctacc gagctggcgg
     2461 cggtcgccgt caagtatcct gagcttcaat ccatgggcgc ggaagtgctc gcgataagtg
     2521 tcgacagcaa gttcagtcac aagatatggc aagaaactga gctatcgaaa atggttgcag
     2581 gcggggtgcc ctatccgatc ctggcggatc agggtggaaa gattggtact ctctacgggg
     2641 tttatgatga ggaagccggt gtaaatgtca gagggcggtt cataatcgat ccagatggag
     2701 ttatccaggc gatggaggta ttgacgccac cagtgggccg taacgtatct gagcttgtta
     2761 ggcaggtaaa tgcttaccag catcatagag ccaccggcga tgtcatgccc tctggctggg
     2821 agcctgggaa gccaacgctc aagccaggac ctagcttagt gggtcgggta tgggaagttt
     2881 ggaagcccga acaggccttc ggtaagcgct aggacatgac gcagtctcgc ggggaaccgt
     2941 gtcgatgggg gccgcaaggg cctccgtcct ttccgtacat tatgggttgt caccaaacgc
     3001 atcacgtaag aatgcgctgg cacatgctgg tgatagaaag ttaaccatcg tttctagcaa
     3061 ctgattgttc gggatcctaa ggcatctcct agaggttcgc agtatatctt gagattgttc
     3121 ttgcaatcaa atgagcattc aaccgtagat gtgctgtgaa aagtcaatag ccgatgaact
     3181 tcagactatt actggcggtc agatccccgt tgatgtaaaa atgccttttg tccaggtgaa
     3241 cagtggatca ccacatcgtt agctcccttg cttaagccgc atctgaggta tctgataacg
     3301 atgatggtaa tagtggctcc gctgattttg ggtacagcgt agccgcgatg atagagcctg
     3361 aacccagagt ctacagtaat ttgtctgtct gagactacat acgccccgtg tatcttcttg
     3421 tttggtttgc tgaagatgta gcttttcgtg tggtgcatgc tactacagtc actgtctcgg
     3481 ttcccacctg cacaacaaag tctctgcctt tcctttaacc agaatctgtc tttatctagt
     3541 tgtaaacctt cttggtgatg cactctgcta tcaagagggc ttgcactcgg cttttgtcgt
     3601 ttgcttgtat ccgatatgtg gatgacgata tcccaaggag tgcgacgccg aagattgcca
     3661 gcagtatcag gcttgccata gatatgagca ttgccgatcc gtgctggcga cgcaagagtc
     3721 ggccatccgc acgcctccca ttattgcctt ataacgactg cagtatcggc gtatggggcc
     3781 ttgattgtgc aagaatcctt tgtctgtttg acagaaccaa gcagtaatct gtattcgcct
     3841 gagccgctag cgcgttggct gagatgtctg tgatgatcag tctctataag gattaggcct
     3901 tcggcgtttc atgaagagcg tctattgtgc gacgcttcta ttcttcctct tgtgatagtc
     3961 acagatatcg cccatgtcct attgggttcg attttagcgg gggctacgtc cgtgtctcga
     4021 ctgaaaactc cgttgccgga agcattgaga agaagtgcga cccgtgcaaa tcgatggact
     4081 caagggcaag attggagcag ggccgcgatt gttacaccta gacgtgatca gagcctcgat
     4141 gcctcgcctt cggggaatgt tatagcatga gcaagttatt gcgcgataat cgcccgggtc
     4201 tggctcgcat ggtacctgtt aatgaaggaa tgctcccgaa acggaagatg aggtcgagtg
     4261 gagatagact ggccggtgcg gaggatttgg catgacgctg acgaagaaat gagcgcacat
     4321 tcgtttaacc atataaggat agatgtaggt ggaatctgat ttgaaagaag agcgagttac
     4381 tgaggcgtcc atctcctctc ttaggaggct tgccaaggag cgtgggtgga caatggagga
     4441 aagagatatc gaacacggtc gtcaggcaag ggtgagcgat ggagagaaca aggcgaccat
     4501 ctcattttac actacaggca aggcactcat tcagggtcga ccatcctcgc tcaccgagga
     4561 actcgattct tggtgggccg gttcgcattt gaatcgcagc gcgtatgtgc cgcctccgaa
     4621 ctcagggcat ggcagtgatg ctaacaagcc ctcctattca gggcgtgcgc gcgccagagg
     4681 ggttgcaagg atcggaattg acgagtcggg gaaaggcgac tattttggcc cactggtagc
     4741 tgcaggtgtt tatgtggacg ctcagacaga gactgagctt ctcagcagtg gcgtgcagga
     4801 tagcaagcgt ctgacggata ggcgcattat ggagctttca gggttgatag tggaaatatg
     4861 tccacattcg gttgtggcca ttggccctgc acgctacaac gggctgtaca agaaaatggg
     4921 caatgtcaat tccatgcttg cgtgggctca tgccagatgc ttggagaacc ttcttgagac
     4981 ggtcagctgt gagcttgcag tgtcggacca gttcggcgac gagtcatatc ttaagaatgc
     5041 tttgatgcat cgagggaggc agataactct ggaacagcgg ccacgggccg aggaggatgt
     5101 tgcggttgca gccgcctcga tactggcccg agccgagttc gttcgcaggt tagagtccct
     5161 ttctcaacag gccggagttg agttgccgaa gggggcatca gatcctcgca ttgtcgaagt
     5221 ggcttcggag atcattgcgc gcgagggacg ggacgctctc gcgaattatg ctaaggttca
     5281 tttcaggact actgacgctc tgggtgtatg acatacctgt gcagaagccc ggcgacgcgg
     5341 cgcaatactg cgatttttgg gggtaggtgt gatgacagaa tatcaacggc cacagcagaa
     5401 gcggagcagg attgtaggta cgctgttgat tgctataggc tgttttcttt tggcggccca
     5461 gtttctcgac accagattgg ttagcctctt ggtactccca ttgcttgggt tcatcttcct
     5521 tgcttggggg actgttgggg cgaacaaggc aatggcaatt ccaggcggca tactattggg
     5581 cattggtaca ggtgtgataa tgtcggagga agtattctgg tatcttgggc aaccggcttc
     5641 aaccggagtc ttcctggttt gtttcgcctt aggctggctc gtggcctata tcgtctctta
     5701 caagaccgga ggcagaaaga catcgtggct gcttgtgtta ggcgtcgttc ttgcggctgt
     5761 cggcattccg cttatccttg ctcagactgc gccggaaatt gttaagtgga ttgccacgta
     5821 ttggcctgca cttttaattg cagcggggat catgctcatc gttcgagggg tcaaataacg
     5881 tgtctgatga gtgtgatata actcgtcctg tgcaatcatg ttttccgcat gagaaagata
     5941 agagggatat ggaggtagac aagatgaaac cgaggattat caaagaaggc gtgacttggg
     6001 tgggcgcggt ggattgggac aggcgcctct tcgattgcct agtccccatt cccgatggga
     6061 ctagctacaa ctcataccta gtacaaggca aagataagac tgtgcttctc gacaccgttg
     6121 accctagcat ggtcgggcaa ctcgtggacc atctgtcgga tatttcaacc atcgactatg
     6181 taatagccca tcatgcggag caagaccatt caggatctat tccgaaaata ctggaaatgt
     6241 atcctggggc caaagtagtc tgcacgccga aatgcgtaga gatgctcgtc gatcttctgc
     6301 atgtagacag gcataggatc atctccgtgg aggacggaga catgatcgat cttggaggac
     6361 ggacattgag gtttgttcac gcgccttggg ttcattggcc tgaaacgatg ttcacatatc
     6421 tggaagaaga caagatcctc ttcacctgcg atttcctcgg ctctcacctt gccacgagcc
     6481 agctctattc gaagaatgag cctgcagagg aggcagctgc caagagatat tttgctgaaa
     6541 taatgatgcc gtttggcagt atgatagtga aaaacctcga taagatttcg gcatacgaca
     6601 tagagattgt ggcgcccagc catggcccga ttcacgacaa tcccgagttc ataatcgaaa
     6661 gatataaaga gtggacctcg gggcccatga agaacgaggt ttgcattgcc tatgtgagca
     6721 tgcatgggag cacggccgca atggtggacg cactagtcga agaattggtt gaacgaggag
     6781 ttggcgtcag gcgattcgag cttaccgggt ctgatacagg cgctattgct atgtcgctgg
     6841 tggatgcggc gacagttgta atagccacgc ctactgtgct cgcaggcgct catcccgctg
     6901 ctgcctatgt ggccttcctc gccaacgctc ttaggccgag gacccgatat ctgtcggtgc
     6961 ttacttccta cagctgggga gggaaggctg ttgagcagat cgcagggatg actacaaatc
     7021 tcagagcaga ggtattggaa ccggtcatct gcaagggact cccaagggag gatgattttg
     7081 cggcgattgc aagacttgcc gagctcatcg cggaaaagca tagggagata gggatactgt
     7141 gatcctgttc gcatctggca agacggtcct tgagtgtcgg agatggaggg gcattgatca
     7201 atggctaatg aacgaataag tgtggtgcaa ggtgacataa ctttggaacg tgtagatgcc
     7261 attgtcaacg cggcaaacag cagcttgctt gggggaggag gcgtagacgg cgccatacat
     7321 cgggctgccg gtcctaagct gcttactgaa tgcagaagga taggaggatg cgatacggga
     7381 gaagctgtcg ttaccggagg atatcgtctg cccgccaaat gggtgataca cacggtcggt
     7441 cccgtatggc atggcggtgg actcggcgaa gcagaattgc tgacgaattg ttatcggaat
     7501 gctctgcgtc ttgctgtaga aaatggtgcg cgcactgtgg cttttccgtc gatcagcact
     7561 ggcgcctatc gttatccggt agacgaggca tcgcgaattg cgattcgtac agtatcggag
     7621 ttcctgtctg tagatgacac catagaggat gtcagattcg tctgttttga cacacatacg
     7681 tattccgcgt acctgagggc tctcgcggag cacctgggca ctcagggcct gccgcggaac
     7741 tcagcgtcgc catgacggag tctgctcgtt agcagattgt ggtcggcatg ttcggcgggt
     7801 taatggtata ctcttgcgtt ggcagtggat ttgtgcaagg gagatatgtg gagtttgccg
     7861 gtgatgtttg gcatcacgat taacacttca ctagcttagc cgtatccggc tttgctcagc
     7921 tcagtctgta gcgactcttc tgtcaaatgg agagcattga ggttttcgta gtgcactttt
     7981 ctctccaact ctaccgtctg agcgaggccg aagaacctta taatgcagct ctcatgacag
     8041 aagatgccat gatgttcgga atgtcaacgc aaaacacata atactatgaa taaaaagttc
     8101 aattgccgta acaagtgtct gagacttcag tggtggagat cgagtaaaaa agaggggaat
     8161 ttcgacgcaa tagcgaggcc gacgtcgttt cccctctact tgtaattaag ttatgagtcc
     8221 gttaattgct gattttggtc agctttctaa ttccccttgg ccagataaag cgaagattcc
     8281 tccgataaga aggattgcgc cgggccaacc gaagtcgcct ttaccaaaga tccagccgag
     8341 aacagcggca ataagcatga tgatgccagc ccatttcggg tacgccattg cacagcaagc
     8401 tgcaatgagt cccgctatgg agataagaac aacaagccat ctcagatcat caactggaac
     8461 gaacataccc cagattgaac caatcagccc gagtatgagt ccggtaactc gcattctagt
     8521 ccctcctcac atttaatcac tcttgtgcct ggttcagtac aaacgcgtcg gcctcgccac
     8581 gtccgatact gaccttggac acaacttgga aaccaaatca tactgcttcc aactattagc
     8641 ttaatcgttc tctccaatac tgtcaaccga agaagatgag ttcttacgca atattgcgca
     8701 aacacgctaa aaaccacgag ataaatgcag aatccacgtc ggttgtaccc ccgaaaaggc
     8761 ggtgggcaag ggaagatctc gtgacatttt ctgttagcgg ggcagcttgg cgtgtatgcc
     8821 cttggggccc tggaggcggc aagagtaatc ttggtgattg agaaggcaag ataacaccgg
     8881 gaatgagggc attgctatag gatacggggt aggcggtgct tgaagctaga tagggagtcg
     8941 ccataagaag ttggaagggc tcataattgc gcagaggttg actgatgaga aggagttttg
     9001 gaggagctgt cgaagtatgc aagcaataca accggttgca aacatcatca tagtaaggac
     9061 agaataccga gatacatagg ttattatgag taaaacaacc gattgctatg ctaggagatg
     9121 ttgcaatgag gccgacgctt caagatatgg cggatattct caatttgtct aaatcaacag
     9181 tgtcaagagc gttgtctggc gatcctcgtg tagcccaagc tacccgcgct agggttgagt
     9241 cccttgccga gagaatcggc tacacgccta acccgattgc tcgagggctt gcgactcgcc
     9301 ggaccaacac aattggtctt gcggttccat gtgcacctcg ctcgctgtct gacccgtttt
     9361 acttggagtt tctagggcat gcgggcaatg cggccatgaa atgcggttat agcttattgc
     9421 tatcggcggc agaagaggac ggtgcagaca ctcataatgc gcatgtgcgg ctggcaagtt
     9481 ccgctaggat cgacgggatg atcctgactg aaccaaagat caaagatgaa cgaattgcgg
     9541 cattaaaaaa ggcgggcatt ccctttgtct tccttgggat gacgtctgat ccagatgtgt
     9601 cgtgggttag ttgtgacaac aggcttggcg ctgctgaagc ggtagcgtgg cttacggggc
     9661 ttgggcatac caggatcgcg tgtcttaccg gtcctgcgga tcaaacggct tccgatgctc
     9721 gatatcgagg ctattgcgag gccctggacg aggctggaat tccccttgac ctcaatatcg
     9781 tggcttcagg ggatttcact caagctggag ggcatgctgc catgagggaa atattggaac
     9841 gcgggaagac tgtgtcagcc gtattcgcct caaatgatgt catggcgctg ggcgctatgt
     9901 gcgctcttcg cgagagtgga ctgtcggtgc cagaggacgt gtcgcttgtt ggctttgatg
     9961 ggatcgcttt cgcgcaatac tcgacgccgc cattggcaac tgtgcagcag cccattgatg
    10021 agttgggaaa tgcttcggtt gaagttctca taaaactgat gtgtggagat aacgggggac
    10081 ctatacatcg ggtgatgccg gcgaggttcc ggcctgctgg ctctgtggct gcgccagggc
    10141 gtgcttttgt gttggaaagc ccggattgaa tgtagtttgc gacgtaccaa gctggaatcc
    10201 gcctacaaga ttggaggtga tgctgcgatc atttcgccga gctcaaccct gtgagtgtca
    10261 gtttttgtaa gcgagcacga gttagccaag aaaactgcga aagcggaggg attacagtga
    10321 agcgaaagat gattgtgtta ctcatccttg cgcttgccat ctgcagtctg ccaggggcca
    10381 ctgctgagaa gaaacttaca gtgattagcg gatggtcagg gcctgagatg gatgcgttca
    10441 tgcccgtgtt gaaggcattt gaaaaggaaa ccggaatccg tgtggactac cagatttatc
    10501 gagcagaaga tttggcggtc ttgcttcctg ctcagtttaa tgccaaaact acgcctgggg
    10561 acgtcatata catgtggtcg tggtttatta ccaaacatgc aaaagatggc catgtgcttg
    10621 agatgaccgg gagccttaag gatgccgact tcattccagg ggcgcttgat gccgtcaagg
    10681 tcgataacaa gctgtggggc ggggtatata ccggcaaggt gaagccgggg ttctggtacc
    10741 ggaaatcgtt tttcaaaaag catggacttt ccgagccaaa aacatgggat gaattcgtcg
    10801 cactctgcgc caaactccag aagatatccg gcgtaaaggc accaattgcc agcggaaatg
    10861 gggttgggtg gcctctgtcc gacattacgg agcatttcat cgctacgtat gccggtcctc
    10921 aggtgacata cggtttgata gacaagaaag tcaagtggac tgaccctgtg gtgaaatccg
    10981 tattcacaga taggattgtt ccgctgctca aagcgggtta ctttagcgaa ccgattgagt
    11041 ggaccatggc tctcgatcta tggtggaggg gagactatgg tctttacttc atgggcagct
    11101 ggatcacggg catggttgat aacccagatg atctgggcgt tttctcttta ccaggatgcg
    11161 aaggcatagt atttgcgcct gactacgctt ttgtgccagc ctacaccaaa tatcctgcag
    11221 aggccagaaa actcctcgcc ttcctctcaa ctaagggtca ggagatacag gtttccacag
    11281 gtggacatat tgctacatac aaggttccgc tcaacgccta tccccctgtt gaccgcgggg
    11341 tagcctccct tctcaaggga gttgttgctc tgcccgacct tgatgatact atcggaggcg
    11401 agtggcaggc ggcgttctgg gatcagctca aactgctctg ggtgagccca ggcagggtca
    11461 atgaggtcct tgagaatctg cagcggaagg ccaattaatc gtaattggta gggctccgga
    11521 gcaaccaatc ttgacaatgt ggcgctccgg agcgcctttt tctcagagca agatgtcagt
    11581 tgggaggtgg cgctatgggt cagacgcgtg gtagaacgct ttttttcatt ccggccctgg
    11641 cattgttggc tctttttgtc gtttatccag tagcaaacac gatatggttg agttttttca
    11701 cgccggaagg cgaattcgca ttcttcaaga attatgcgga tgtcctctct cagccggaga
    11761 tattcgaccc gcgagggttc gaacggggct tcccttatgg ggcgttgatt cacaacgtca
    11821 tatggatagt gttacacctg ccccttaccg tgatattggg gttggtattc gcagtcattc
    11881 tcagagatat caagggtgga gccatagtca aatcaatagt ctttcttgga atggtcaccc
    11941 ccatggttgt gggaggcata atcttgagat tcctttttga tgggaatgtc ggaatcatcc
    12001 catcagtact ggcccttttc ggttttgcgc cgcgaactct cacagcctat ccggataccg
    12061 ctctcttttc ccttatagcc gggtcgcttt ggctgtggac tccgttcagc atggtgcttt
    12121 attcggcagg cctggagact atacctgact ttgtgtatga agccgcagtg atagacggtg
    12181 cttcgccttg gagaatattt ttcaatatca ctgtgccttt gctcaaacca gtcaccggtg
    12241 tcgtggttac catgacattc ctgtgggttc tcaagatctt tgatatcgtt ttcgttgcta
    12301 ccatgggagg accgggaggg gcgtcgaacg tgctggcgtt gcagatgtac atgtatgcct
    12361 tccgtgagtt caatccgaat gccgcggccg ttgtggcgac gttcctcatg attgtagctc
    12421 ttgtggtgtc catacctact ttcaaggctg cagggggtga agaatgatga gaaccctagg
    12481 atgcagcagt gggcacaaag tgattacgta tgtggctgct gggctcatag cgctcatatg
    12541 gcttatccct tttttgggag tcttcatggc ctcaataagg cctttgtcgg agataaacag
    12601 gggatggtgg aggtttgatt cgttcactcc aaccttcaaa aactatgtag gcgcttggaa
    12661 tcatcctgca gctccgattt ctgagggcat gcttaactca ctcaaggtgg cgattcctgc
    12721 cactatcata cctgtatttg tagcgtcgat cgcggcatat ggatttgcgc gtttcaaatt
    12781 ccgggggaga gatcagctct tcattctagt cgtagcgctg atgactttgc cgcagcagat
    12841 gatagcggtg ccgatgttcc agatgatgaa caagcttggc gcggttgatt cgtattcagg
    12901 cctaattctg gtccactcgg cttggggaat tccatgggct ctctacttca tgagaaatta
    12961 ctttaccacg ctgcccattg aagtggagga agctgcgaag gtggatgggg catcagactg
    13021 gaggatcttc ttccagatca tcatgcccat ggcgcttccc gccatcgcct cggcggcggt
    13081 attgcagttc atgtgggtat ggagcgattt ctttcttgcg ttgatcctca tctattcgcc
    13141 tgcgaaactc cttgctacac aacgtgtgcc gctgcttcgc gggatctacc atgtggactg
    13201 gggtattcta gctgcaggca gcataattgt gatggctgtt cctattattg tcttcgccgt
    13261 catgcagaga tattacatca gaggcatggt cggctgggtg tcaaaatgac agattgtgat
    13321 tactaggcta tgggagggca atgtatgttc cgcaaagtcg atttcttgga aaaatccctt
    13381 gacgaccatg cgccatatgc aggagaagac aggattgccg agatcagacg tcttgctgaa
    13441 gacctcggca gcctgcgggt gcttcatgta aattctactt cttacggggg aggtgtggcc
    13501 gaacttcttt acaccatagt cccgctgatg cgcgatgccg gacttgacgc tgagtggcat
    13561 gtgatcgatg gcgcgcctga ccagttcttt gatatcacaa agaagataca caactccttg
    13621 caaggggcaa acactccgct gagcgatggc gagtggcgac tgtacgagga cataaacagg
    13681 aagttggccg aggcattccc cagaggccct tgggacatag tcgtgatcca tgatccccaa
    13741 cccttggccg tgttgacctt cctggattcg ctttccgaat cagatcgccc cgttgttggc
    13801 aggtggtttt ggcgatgcca catcgacctg tcaacgccat tggaagagac gtggcagaag
    13861 ctctggccat acgtgaatgc atatgatgga gcgatcgtta cgagccgaca atacgcgcgg
    13921 gacgagatga cgcttccggt tgctgagata actccgtcca cggatcccac tagcccaaag
    13981 aatctcccta ttccagaggg cgaagcaagg aaaatgatgg gctcatttgg cgtagatcct
    14041 gctagaccca tcattgtgca ggtatcaagg tttgatcctt ggaaggatcc gtttggcgtg
    14101 gtggaagcgt atcgaatgct caagggaaaa tggcctgagc tccaacttgc aatggtcggt
    14161 tctatcgctg ccgacgaccc cgaaggcatc gggatacttg cacacttgaa agaggaggcg
    14221 gctggggaca ggaacataca tgtgctgtcg aatgaggacg gtgtcggcgc gcgggaggtg
    14281 gctgcattcc agcaatgcgc agatgtggtc atacaaaagt ctatccgcga ggggtttggc
    14341 cttacgataa ctgaggctat gtggaagcga cgacctgttg tcgcaggcaa tgccactgga
    14401 tgcaagattc agataaccga cggcgtggat ggttttatag ttgggactac tcagcagtgc
    14461 gctgaaagga ttgatgagat actgtccgat ccggaccttg gggccaggct tggtgacgcg
    14521 gctcgggaga cagtgaggcg aaggtttctt tcaacgcagc acgttgcgaa ctacctgaac
    14581 cttttcgctg gttgccaata accggccaca tgttttggtc agaaaccgtt gtttaattgc
    14641 gtatgctgct ccagggtgat gatgttgccc tggagctttg ctctatggtg tcctggcatt
    14701 tcactcttgg gggacgggcg attttgagaa gtcgtacgaa tttggcgatg gtcgatgctg
    14761 attttcggac gggatctgcc agtttgctca tcaagtctag ataggctctt gcgcgatcct
    14821 cgagtttgcg ttgtctacaa ttcggacctc agcctctcat aagcttcttc ttgtgagtta
    14881 tccagagcta tcggctcggt gatggttata cggccaatag tctggacaaa taggaatcat
    14941 cagcgtaggt tccccttgtc aatatgttgg attagaaatc cgcagatagg cataacccat
    15001 gtagctggtg ccaagtgttg aagcgtcgca aagcctttct agcgattgtt tgtttcagct
    15061 gtgggtgacc taccattgcg caattcagg
//
LOCUS       DAOSYL010000057        14966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3366_length_14966_cov_7.749514, whole genome shotgun sequence.
ACCESSION   DAOSYL010000057 DAOSYL010000000
VERSION     DAOSYL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_14966_cov_7.749514"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(520..1995)
                     /locus_tag="PLU15_08680"
     CDS             complement(520..1995)
                     /locus_tag="PLU15_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO31035.1"
                     /translation="MRINHNLSALNAWKNMSVNDTGQNKSLEKLSSGLRIGRAADDAA
                     GLSISEKMRGQISGLNQASRNAQDGISLLQTAEGALQETHSILQRMRELAVQAASDTV
                     TNEDRLEIQKEIDNLRVEMDRISTDTEFNTQKLLNGKFANKIIHIGANAQQNLKVSIA
                     SMGARDLKVNKVTTASSATTLNDASFIAEHAGDATLTVSGTYTGQAPITALRTSITED
                     GKVRVIGKRDGAEGDLIVDLDSGATSVEVNGLTFAIANIADVRANATTIEGGPDDGKI
                     DNTGDAGTATQFSFINGDGATLAMSGTATENITDISATVVGDGQVQISYTIGSDTTSV
                     TLYGDDTSATAEHTVAGVTFELTGAKNVREAPTTFTWEDDNLPINAEVVGGLDVVDEG
                     INVSTQAAADAAITVIDEAIVTVSGERSKLGAIQNRLEHTIANLSTSSENLTAAESRI
                     RDVDIAVEMMNFTKYQILSQASTAMLAQANAKPQAVLQLLR"
     gene            complement(2114..2581)
                     /locus_tag="PLU15_08685"
     CDS             complement(2114..2581)
                     /locus_tag="PLU15_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOO31036.1"
                     /translation="MKVQSSRFGALTVDDSKLISFPKGIPGFEDSRRFFLLDHPGSAK
                     VKWLHSADDPGLALVVASPFELFASYEPEVPDGVAEALGAEGPEDMLVLTVLSVHAGN
                     PPKVTANLLAPIVVCSATMRGMQVVMEAGKYGVRHEMELVPEKCEAGAKSSVG"
     gene            complement(2603..3643)
                     /locus_tag="PLU15_08690"
     CDS             complement(2603..3643)
                     /locus_tag="PLU15_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO31037.1"
                     /translation="MRRIAVEHMKPGMTLAEGIYNGSGVTQVSPGSVLSETAIARIRQ
                     LGISHVFIEDPRTDDLVIRDAVPWNVAARGRELLRDFEERVREAGGPDHVKVPFDDLL
                     ELVHDIEDGLQGTDLNVLEIPPVTRPEDYRYAYPINVAALSMFVTAKAGLPRRTTDVG
                     LAALLLDIGMELAPEEPEKHPEMSLAVLRQDERFSAYSRAIVFQHEERHDGSGYPRGI
                     AGDDIDPLAKIVSVADMYCSLISEGNPMGERLAPQEAFDYVTSAAGFEFERTTVLGVM
                     QYVALYPVGTMVRLNTGECGVVTRVYKGLTTRPTVRVLYGADGVEVARPYEMVLAEPE
                     HQTVLVAGVCED"
     gene            complement(3640..4452)
                     /locus_tag="PLU15_08695"
     CDS             complement(3640..4452)
                     /locus_tag="PLU15_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOO31038.1"
                     /translation="MSLVDNADYEVFRRKVLALTGIDLGLYKSQQMQRRIGSLMERAG
                     VSTFAAYGDLLGRDRAELDRFCDYITINVSEFFRNPEKFDELEREILPELLARSRNLS
                     IWSAGCSNGAEIYSVAMILQEIAPRGYHRLLGTDIDTGILSKALAGVYGPQDVRNVSA
                     ARMAKYFKADGESYVLSPEIRSKVQFKMHNLLSDPFERGFDLIICRNVVIYFTDEAKQ
                     DLYGKFWESLKDDGVLFIGGTETILNAKDIGFAPMASFFYKKVPVRKEVGVG"
     gene            complement(4494..5561)
                     /locus_tag="PLU15_08700"
     CDS             complement(4494..5561)
                     /locus_tag="PLU15_08700"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOO31039.1"
                     /translation="MMPISVLVVDDSAFMRKVVSEMIASDPELSVAGTARDGKDAIEK
                     ARSVRPDVITLDVEMPVMDGLEALPVLAGELGIPVVMLSSLTQEGAEVTLRALGLGAI
                     DFVPKPSGAISLDIDVVREDLLTKVKAAAKVSSARVRGLARDARVPLTGLGREVKEAK
                     SGARAARARNVVVIGTSTGGPRALTQVVPWLPPNLPAGVLIVQHMPAGFTKSLADRLN
                     QTSKIRVLEAQGGEAITEGAALIAQGGLHMVVEPDGTVRLDSSPPMHGVRPAVDLTMN
                     SAVDLFGKKTVGVIMTGMGSDGADAMARVKRMGGKTVCEHESTCVVYGMPRAVVERGD
                     ADRVVPLPKIADEIVDAVLSL"
     gene            complement(5561..5917)
                     /locus_tag="PLU15_08705"
     CDS             complement(5561..5917)
                     /locus_tag="PLU15_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO31040.1"
                     /translation="MPTVLVVDDAAFMRMRAGKLLSQSGYEVIEAENGLDALNKYTEY
                     EPDLVLMDITMPVMDGLTAVREIRQVDPEAKIIMVSAMGQQAMVIEAIKLGAKDFIVK
                     PYEPDNVLASVKKLIG"
     gene            complement(5940..6449)
                     /locus_tag="PLU15_08710"
     CDS             complement(5940..6449)
                     /locus_tag="PLU15_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOO31041.1"
                     /translation="MALNGAKDVVPIGIGEMHVSDKPGDVLVAYGLGSCVGVVIHDPI
                     GKVGGMVHVALPESLSAPRSEERPDARYADVGVPMLVGEVLRLGGMKARLVARIAGGA
                     KMFDVTPELEFMDIGARNSVAVRAELEAMGIPIVAEDIGGADGRTLRYVVGREQVFVR
                     KLGMREVEL"
     gene            complement(6430..8601)
                     /locus_tag="PLU15_08715"
     CDS             complement(6430..8601)
                     /locus_tag="PLU15_08715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOO31042.1"
                     /translation="MLEFPGMTADDIKVFLIEAEEQLQSLEDGLLELEQAGEDDEVIG
                     QIFRAAHTLKGSSATLGHDKMANLTHSMESVLDLVRKSQLEVGPDLMDVLFECLDVLR
                     VLNREVETGQESGEDITVLTGRLLDIIAFAEEGGSGCISGESEAGGSGAQSTVAAVDG
                     GIMHGPGDVELRIAFADTCQMPSVRAYQVVDRLSVMGNIIYTRPSMELIEAGQDIGEL
                     TVAIEWDGDSPQPLVEAAMDVSDVESALVVQVRPAGSLKGEADAAAEASRATVASGPK
                     ESEPTGVEAGRKAGVNRGSRSATSTRATTVRVDVARLDALSNLVAELVIDRTHLAQLE
                     SKLAEKHGGDELVAELNRTSTHIGRLTTELQEAINKARMFPIDNVFRRFPRMVRDLAQ
                     SQGKEIDFIIEGEDTELDRSVSEEIGDPLIHLIRNAVGHGIETPEERVAAGKAPKGTV
                     RLSAFHQENYIVIEIADDGRGIDPEAVKESAIRKGLISEEAAARMSDRDAVNLIFSPG
                     LSTSKKVDDVSGRGVGMDIVRKNIERLNGSISINTRLGEGSTFTVKLPLTLAIIRALL
                     VAYDSRIYAIPLASVMEITRIEPSDIHTVHKREAIRLRGDVIPLLWLGEIFSKRGEER
                     GAAEWMAADGRPEDGAAGEAQSRRMFAVITSSHEGQVGLVVDSLVGEMEIVIKSLGSF
                     IGNIPGVSGVTILGDGRVALILDIPSLVRRVVEERNGAERG"
     gene            complement(8613..9095)
                     /locus_tag="PLU15_08720"
     CDS             complement(8613..9095)
                     /locus_tag="PLU15_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO31043.1"
                     /translation="MEHGFDAEEIQLVVFELADESYGVEISRVQDINRMQEITEIPHA
                     PESVVGVINLRGRVIPVVDLRTRFGLPPAEHTKSTRIVVVQMGEEPIGMIVDAVSQVL
                     RIPTKIIEPPSPVLSGVDSRYLRGIAKLEDELVILLDLDYVLSKDEQEFISEAMAESV
                     "
     gene            complement(9109..10017)
                     /gene="flgL"
                     /locus_tag="PLU15_08725"
     CDS             complement(9109..10017)
                     /gene="flgL"
                     /locus_tag="PLU15_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOO31044.1"
                     /translation="MRVTNNVMMERAMRNVNVAFERYVKRQEQLASGCSINRLSDDPH
                     GAAKAVRLRSTLKGLEQYKTNADIADTKLRAYDGVLDKVGSIMNRLRDLVGRAASDTV
                     TPTERDGIAVEVRELFEEVVNAGNEMHNGEHMFAGHKVGQAPFKVERDADGRVTGVLY
                     HGDSGKDAIEIGPGIVISGSLAGDEVFMADGANVFDAMIDIERHLRENDVESLSEEDL
                     GRVDAVVDNILKHRAEIGGKGKRLELVMDRLESDSISIEGLLSKTEDIDFAEAIMRLC
                     EQEIVYYAALSAASKSVQAGLLQFLR"
     gene            complement(10094..11422)
                     /gene="flgK"
                     /locus_tag="PLU15_08730"
     CDS             complement(10094..11422)
                     /gene="flgK"
                     /locus_tag="PLU15_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOO31045.1"
                     /translation="MYSTFVGLETARRALYAQRAAIDITGHNVSNVNTPGYRRQRPVI
                     EATPPAPGALQPGTGVKVAGIERMRDQFVDTQIRHTLASLGRWEARQEILEEIQEVFK
                     EPSDIGISAMMDRFWISWQELSGNATDPGRRTSVQQEAISLASAFNLAAAQLNETKAD
                     IDQNIKSLVHRVNTTASSIAELNREIVRGNVVGENVADLLDRRDYLVQQLASDVDVAV
                     VEDNSGLVHVTVGGRILVAGTDARELAEDALITGGRIKGLNEAANMIIPGYLKELDDL
                     ANALIEKINEQHMAGYDLFGNSGDVFFEGEGAAGIRLSEEIEGNPGLIAAADQTDSPG
                     NGRNALAMAQLKDGLTMVGGTATFRDFYGSILTGLGLETQESMRSREVYEGVLEQQKA
                     RQESVSGVSLDEEMIMLIKYSSAFDAAARLVTAVDEMLDRLINGTGLVGR"
     gene            complement(11448..11948)
                     /locus_tag="PLU15_08735"
     CDS             complement(11448..11948)
                     /locus_tag="PLU15_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOO31046.1"
                     /translation="MDYAKEMARILNREFALCSELLYVARAQRSVITSGDVAALAELV
                     SRAEEIIGAIRDEEIGLADLAKRWADETEVEVEDAEQALAVMIALFKEEEMAEFEVLR
                     DSIAGILAEIDSANAINAMLIQDAIEYIENTVRLIAEADGTNSVYSRLGRMDKKAVSS
                     AVDDTA"
     gene            complement(11914..12237)
                     /gene="flgM"
                     /locus_tag="PLU15_08740"
     CDS             complement(11914..12237)
                     /gene="flgM"
                     /locus_tag="PLU15_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOO31047.1"
                     /translation="MKISGSRGFDRINLYSQLAKKAERAERTGEVKPDEVEISDRGKE
                     IHHAMQALSSVSDVRDDKVEAVRSRIESGTYEVSGKDVARKLFAGAVRRGDLGGLRKG
                     NGEDS"
     gene            complement(12316..12780)
                     /locus_tag="PLU15_08745"
     CDS             complement(12316..12780)
                     /locus_tag="PLU15_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31048.1"
                     /translation="MALENCSRCGKLFHRISKPLCPDCLVEAEKLAEKVVEYLRRHSG
                     ATLTEIADATGVDEAFVLDLLRDGRIEMASDVRPVLFCRACGASIESGVYCAKCLDKF
                     GKAFSGRGCPGGQTGLGRGDDGEKSRYKRAQLGERASGARMSVPERRRKGRE"
     gene            complement(12761..13195)
                     /locus_tag="PLU15_08750"
     CDS             complement(12761..13195)
                     /locus_tag="PLU15_08750"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOO31049.1"
                     /translation="MGDVSPVGMDLGTAGTMMRLSLPYGLAAGAGDRRNLAKSVELET
                     AGGSDAAAEAGARARENERLAQACSEFEAIFIEMILKSARASIPKGGLFSSEEEELVR
                     EMLDSQLAQEVAKGGGIGLAKFMERNMTGDGKAVKQGGAGEL"
     gene            complement(13217..14380)
                     /locus_tag="PLU15_08755"
     CDS             complement(13217..14380)
                     /locus_tag="PLU15_08755"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOO31050.1"
                     /translation="MRRRRSGLFKIAAWLALLLMSCSIPRVYGAQPTEVVESQASQGE
                     GSSQPALTGSAGKDGATVRVKDITRVYGVRDNQLIGYGLVVGLKGTGDSRNTPFTART
                     LSNMLDKFKITVDPLEIKAKNVAAVMVTGVLPAFASPGDAIDVAVSSVGDARSLAGGT
                     LLLTELAGADGRVYAVAQGPVVTGSEAKATVGRVPAGATVERHVPMEMLTPDGHLVLS
                     LLNPDYTTAARIARAVCDRFGQGAAVAVDGGTVRVSVPEWLQETPTPFIAEVGELEVA
                     PDAPAKVVLNARTGVVVIGGNVKVSAAAVSYNGFNVSVGGLPGWQDGAGASSGNVVAM
                     PAQPGSTITLEAGSTIEALAAALNAVGARPTDIMNIMEALRACGALQAELQIM"
     gene            complement(14398..>14966)
                     /locus_tag="PLU15_08760"
     CDS             complement(14398..>14966)
                     /locus_tag="PLU15_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOO31051.1"
                     /translation="VLVFSGGVVRATSLYSEDASPFASMYADRKARQVGDLVTILVAE
                     VSYAQNRSQTNTAQGIGVTAQPGVGVFDFIPAARLDVGDSSKGANETTRSGRLVGTMT
                     AEVIEVLPNGDLVIRGSREVSVNREKEIMVVEGVVRPQDIGPNNTIPSTMVANAQISM
                     SGGVYTGQKGGFFKTLWDGLVWVWNTIF"
BASE COUNT         3173 a         5028 c         3401 g         3364 t
ORIGIN      
        1 attgcgatgt agcactgtgt gaaatcccgc ccggcacatg ctaatctagt tccagagcgc
       61 cagtctttcc ccggagaagc cgatacagac tcaccacaca accatgtctg cgcaagcatc
      121 tcgtttgcca aagagtggat agatgccatc ctcaactgca gggatcacat catgggttca
      181 tttgacgaaa tgggcaggtt tgctgcagcg caagtactct caccaagata cagtaatagg
      241 aatcgccaaa aaccggttta gtaaggcttg acaccagcat gcagtctctg ctgaggacgg
      301 cggccgggca aagcggagtt gtctagaccg caagaccagt ttcaaacctg tgatcggcag
      361 cttctctagt gcacattgcg atctattcat caactccatg ggaacggcga tatcctctac
      421 caccgactac cccgcataga tgcaagagaa ctatgagggg cagcccgaag gccgccccat
      481 atcgtccacc ataccactac aactacgtga cttcccccgc tacctcagca gctgcagtac
      541 tgcctggggc ttcgcattgg cctgggccaa catggcggtc gacgcctggc tcagaatctg
      601 atacttcgtg aagttcatca tctcaactgc gatatcgacg tcgcggatgc ggctttcggc
      661 agccgtcagg ttctcagacg aagtcgatag gttcgcgatg gtgtgctcaa gcctgttttg
      721 aatagcaccg agctttgatc gctcacccga aacggttacg atagcttcgt cgatcacagt
      781 gattgcagcg tcagctgcag cctgagtcga cacattgatt ccttcgtcta ctacgtctag
      841 accgcccact acttcggcat tgatcggcaa gttatcgtct tcccaggtaa acgtagtcgg
      901 ggcttccctt acattcttag caccggttag ttcgaacgtc acccctgcga ctgtatgctc
      961 ggcagtagcg ctagtatcat caccatacag tgtcacactc gtcgtatcgc tgccaatcgt
     1021 gtatgagatc tgcacctgcc cgtcaccgac cacagtagca gagatgtctg ttatattctc
     1081 cgtggccgtg ccagacatcg cgagagtcgc gccatctccg tttatgaatg agaactgtgt
     1141 cgcggtcccc gcatcacctg tattgtctat cttgccatcg tcgggcccgc cctctatcgt
     1201 cgtcgcattg gccctcacat ctgcgatgtt ggctatggcg aacgtcaagc cgttcacttc
     1261 cacgctcgtc gcaccgctat ccaggtcaac tatcaggtct ccctcggccc catcacgctt
     1321 ccctatgact ctaaccttgc catcctcggt gatgcttgtc ctcagagccg tgatgggagc
     1381 ttgtccagtg tatgtcccac tcactgtgag ggtggcatcg ccagcgtgct cagctatgaa
     1441 ggatgcatcg ttcaacgtag tggcactcga ggctgtggtt accttgttca cctttagatc
     1501 cctagcgccc atcgaagcta tgctcacctt gagattctgc tgcgcattag cgccaatatg
     1561 gatgatcttg ttggcgaact tgccattgag aagtttctgc gtgttgaact cagtatccgt
     1621 cgagattctg tccatctcga ctcgcaggtt gtcaatctcc ttctggatct caaggcggtc
     1681 ttcattggtc actgtatccg acgccgcctg cactgccagc tctctcatcc tctgtaggat
     1741 tgagtgggtc tcctggagtg cgccttctgc ggtctgcagg agggagatgc cgtcctgggc
     1801 gttccggctg gcctggttta ggcctgaaat ctgaccacgc atcttctctg aaatcgagag
     1861 tccagctgcg tcgtccgccg cccttccaat cctcaaacca cttgacaatt tctcgagcga
     1921 cttgttctga ccggtgtcgt tcacactcat gttcttccac gcattgagcg cagaaaggtt
     1981 gtggttaatc ctcaattatg tctacctcct tgtagtctat atactggccg ccgcatcctt
     2041 gcggcaggcc acggcttatg cctttgcctg caggacttag gctctatagc cgccttcaat
     2101 atctctatcg acactaaccg accgaagatt tagcccccgc ttcacatttt tccggtacaa
     2161 gctccatttc gtgcctcacg ccgtatttgc cggcctccat tacaacctgc atgccccgca
     2221 tggtagcgga gcataccaca atcggggcta agagattggc cgtcaccttg ggcgggttcc
     2281 ccgcatgcac tgagagcacc gtaagcacca acatgtcctc aggcccttcg gccccgagcg
     2341 cctccgctac cccatccggc acctccggct cgtaacttgc aaaaagctcg aagggacttg
     2401 ccaccaccaa cgccagcccc ggatcgtctg cactgtgtag ccacttcacc ttcgcgctcc
     2461 ccggatgatc caataggaag aaccttcgag agtcctcaaa cccgggtatc cccttcggga
     2521 aactaatgag cttgctatcg tctacggtca aagcgccaaa tcgagagctc tgaaccttca
     2581 ttgcgatccc cccaagccct ttctaatctt cgcagacccc tgcaaccagc accgtctggt
     2641 gctccggctc cgccaacacc atctcatagg gccgcgcaac ctccacacca tctgcaccat
     2701 agagcacccg caccgtgggc ctcgttgtca gccctttgta gacccgggtt actacaccac
     2761 actccccggt gttaagccgc accatcgtgc ccaccgggta cagcgccaca tactgcatca
     2821 ccccgagcac cgtagtgcgc tcgaactcga acccggccgc gctggtcacg taatcgaagg
     2881 cttcctgcgg ggcaagcctc tcgcccatgg ggttgccctc agaaatgagg ctacagtaca
     2941 tatcggcaac tgatacgatt ttcgccaggg ggtcgatgtc atccccggca atcccccgag
     3001 gatatccgct cccatcatgc ctctcctcat gctgaaatac tatggccctg ctgtaggccg
     3061 aaaacctctc gtcctgcctg agcactgcaa gggacatctc cggatgcttc tcgggctcct
     3121 cgggtgcaag ctccatgcct atgtcgagca gaagcgccgc cagccccaca tcggtcgtgc
     3181 gccgcggcaa ccccgccttg gcagtgacga acatggacaa cgccgcaaca ttaatgggat
     3241 aagcataccg atagtcctct ggtcgggtta ccgggggtat ctccaagaca ttcaagtcgg
     3301 taccctgcag cccgtcctcg atatcatgga ccagctcgag cagatcgtcg aagggcactt
     3361 tgacatggtc cggacccccg gcctcccgaa cgcgttcctc gaaatcccgc agcaactccc
     3421 tgcccctagc agccacattc cacgggaccg catcccgtat gaccagatca tccgtgcgag
     3481 ggtcctctat gaagacatgt gaaatgccaa gctgccggat cctggcgatc gccgtttcac
     3541 tcagaaccga gcctggggaa acctgagtca cgcccgagcc gttgtatatc ccttcagcca
     3601 gtgtcattcc tggcttcata tgctccactg ctatccttct catcccacgc caacttcctt
     3661 ccgaacagga accttcttat agaaaaacga cgccatcggc gcaaatccta tgtcttttgc
     3721 attgagaata gtctcagtcc ctccgataaa caacactccg tcatccttga ggctttccca
     3781 gaacttgccg taaagatctt gcttcgcttc atctgtgaag tagatcacga catttctgca
     3841 gattatcagg tcaaaacctc tctcaaaggg atccgacaac aggttgtgca tcttaaattg
     3901 caccttcgac cttatttcag gcgaaagcac gtacgactcc ccgtccgcct taaagtactt
     3961 ggccatcctc gccgccgaca cgtttcgcac gtcctgtggg ccatagactc ctgctagtgc
     4021 ctttgaaaga atcccggtat cgatgtcggt tccgaggagg cgatggtatc ctctaggagc
     4081 tatctcctga aggatcatgg ccaccgaata gatctctgca ccattggaac accctgcgct
     4141 ccaaatactg aggtttcgag acctcgcaag gagctcaggc agtatctccc gctcaagttc
     4201 atcgaatttc tcgggattcc ggaaaaactc cgacacattt atcgttatgt agtcgcagaa
     4261 ccgatccagc tctgcccgat cgcgccccaa taggtcgcca taggcagcga acgtggaaac
     4321 accagccctt tccatcaggc tccctatgcg cctctgcatc tgctgggact tataaagtcc
     4381 gaggtcaatt ccggtcagcg caagtacctt cctgcggaag acctcgtaat ctgcgttgtc
     4441 aacaaggctc atatgcaata cctcacgggc tcactcaagc ataacttcag tgattataat
     4501 gaaagaacag catctacaat ctcatcggca atcttaggga gcggaaccac cctgtcggca
     4561 tctccccgct caacaactgc ccttggcatg ccgtatacca cgcaagtgga ctcgtgttcg
     4621 cacacggtct tgccgcccat ccgcttgacc cgcgccatgg cgtcggcccc gtcagatccc
     4681 atcccggtca ttatcacacc caccgtcttt ttcccaaaaa ggtcgacggc ggaattcata
     4741 gtcaaatcga cggcaggcct cacaccatgc atgggcggac tcgagtctag cctaacagta
     4801 ccatcaggct ccaccaccat atgaagccct ccctgagcta tgagcgccgc accttcagtt
     4861 atggcctcgc ccccctgggc ctcaagcacc cttatcttcg acgtctggtt caacctgtcg
     4921 gccaaagact tcgtgaaccc cgccggcatg tgttgcacaa tgagcacccc ggcaggcaga
     4981 tttggaggaa gccatggcac cacttgcgta agggccctcg gacctccggt tgaagtcccg
     5041 atcacaacta catttcttgc ccgggccgcc ctcgccccgg acttggcctc tttaacctcg
     5101 cggcccaacc ccgtcaatgg aacccgcgcg tccctcgcaa gcccccgcac ccgtgcagac
     5161 gacaccttcg ctgccgcctt caccttggtt agcaggtctt ctctgacaac gtcgatgtcg
     5221 agggagattg cgcctgaagg cttgggcaca aaatcgatgg ctccaaggcc gagcgcccga
     5281 agggttacct ccgccccctc ctgcgtgagg ctggaaagca tcaccactgg tatcccaagc
     5341 tccccggcta gcactggcag agcttcgagc ccgtccatga cgggcatctc cacatccaaa
     5401 gtgatcacat caggtctcac ggacctagcc ttctctatgg cgtccttgcc gtccctggcc
     5461 gtgcccgcca cagaaagctc cggatcggag gcaatcatct ccgatacgac cttgcgcatg
     5521 aatgcagagt catcgacaac cagcactgat atgggcatca tcagcctatc aacttcttca
     5581 ccgaagccag gacattatca ggctcataag gcttcacgat gaaatccttg gcccccaatt
     5641 tgatcgcctc gatcaccata gcctgctgcc ccatggcaga taccattatg atcttcgcct
     5701 ccggatcgac ctggcgtatc tcccgcaccg cggtcagacc gtccataaca ggcatggtta
     5761 tgtccataag gaccaagtca ggctcgtact ccgtgtactt gtttaacgca tcgagcccgt
     5821 tttctgcctc aatcacctcg tagccactct ggctcaagag tttcccggcc ctcattctca
     5881 tgaaagcagc atcatctaca acaaggacag tcggcacctg ccaaacaccc ccactgaagc
     5941 taaagctcca cctctcgcat cccaagcttt cggacaaaaa cctgctccct gccgacaaca
     6001 tagcgaagtg tgcgcccgtc ggccccgcct atgtcctcgg cgacaatcgg aatccccatc
     6061 gcctcaagct ccgcccttac agctacggaa ttgcgcgccc cgatatccat gaattccaac
     6121 tcaggtgtga cgtcaaacat ctttgcccct ccggcgatcc gggccacgag cctcgccttc
     6181 attcctccga gcctcagcac ctcgcctacg agcatgggaa cgcccacatc cgcgtatctc
     6241 gcatcaggac gttcctcgct ccttggggcg gaaagcgact cgggcaaagc cacatggacc
     6301 attcctccga ccttcccgat agggtcatga atgaccacgc ccacacacga tccgagcccg
     6361 tatgcaacaa gaacatcccc tggcttgtcc gacacatgca tctcgccaat tccgatcggt
     6421 actacatctt tagccccgtt cagcgccatt tctctcttcc accactctcc tgacaaggct
     6481 gggaatatcc agtatcagcg ctaccctccc atctcccagg atagtaacgc cggatacccc
     6541 cggaatgttt ccaataaaag aacccaggct tttgatgact atctccatct cgcccaccaa
     6601 agaatcgaca acgaggccga cttggccttc atgagaagag gtgatcaccg cgaacatccg
     6661 tctcgactgc gcctctcctg cagcgccatc ctcaggccga ccgtcagctg ccatccattc
     6721 agccgccccg cgctcctcgc ctcttttgga aaagatctca cccagccata gcaggggtat
     6781 tacatctccc ctcagccgta tggcctcgcg cttgtgcacc gtatggatgt cagaaggctc
     6841 tatcctagtg atctccatca ccgaagccag aggaatcgcg taaattcgag aatcgtaggc
     6901 gaccagcagc gccctgatta tcgccagggt gagcggaagt ttcacagtaa aagtggagcc
     6961 ttctcccaaa cgtgtattta tggaaatgga gccattgagc cgttctatgt tctttcggac
     7021 aatatccatg cccacgccac gcccggagac atcatccacc ttcttgctag tggaaagccc
     7081 cggcgaaaag atgaggttga ccgcgtcccg gtcagacatc ctcgccgctg cctcctccga
     7141 gatcaggcct tttcggatag cggattcctt gaccgcctca ggatctatcc cccgcccgtc
     7201 atcggcaatt tctatgacta tgtaattttc ctgatgaaaa gcagagagcc tcacagtccc
     7261 tttgggcgcc tttcccgcgg cgactctctc ctccggcgtc tctataccgt ggcccacagc
     7321 gtttcggatc aagtggatca gaggatcccc tatctcctcc gagactgatc tgtcgagctc
     7381 tgtatcttca ccctctatga tgaagtcgat ctcttttccc tggctctgcg cgagatccct
     7441 caccattctc ggaaaacgcc tgaaaacatt gtctatcggg aacattctgg ccttgtttat
     7501 ggcctcctga agctcagtgg tgagcctccc tatgtgagtg gatgtcctgt tcagctccgc
     7561 cacaagctcg tccccgccat gtttctccgc caacttcgat tcgagctgag ccaggtgcgt
     7621 gcggtcgatc accaactccg ccacgagatt cgacaacgcg tcgagccgcg ccacatccac
     7681 cctcacagta gtggcccgag tcgacgttgc agaccttgaa cccctattca caccggcctt
     7741 acgcccagcc tcaacacccg tgggctcact ttccttgggc cctgaagcca cagtcgcgcg
     7801 cgacgcctcc gcagcagcat cggcctctcc ctttagcgag cctgcgggcc tcacctgaac
     7861 taccagagcc gactctacat ctgaaacatc catagcggcc tcaacaaggg gctgcggcga
     7921 atcgccgtcc cactcaatgg ccacagtcag ctcacctatg tcctgccccg cctcgatcag
     7981 ttccatcgaa ggcctggtat agattatatt ccccatcacc gacagcctat cgacaacctg
     8041 gtaagccctg accgaaggca tctgacaagt atctgcgaaa gcgatgcgaa gctccacatc
     8101 gcccgggccg tgcataatgc cgccatcaac ggcagccact gtagactggg cgccggatcc
     8161 ccccgcttcg ctttcaccac tgatgcaacc cgagcctccc tcttcggcaa atgcgattat
     8221 gtcgagcagg cgcccggtaa gcacggtaat gtcctctccg ctctcttggc ccgtctcgac
     8281 ttcacgattg agcactctga gcacgtccaa gcattcaaag aggacatcca tgagatcagg
     8341 gcccacctcg agctgcgatt ttcgaaccag gtcgagcaca ctctccatag aatgtgtcag
     8401 attagccatt ttgtcgtggc caagtgtggc agacgaaccc ttgagagtat gcgcagctct
     8461 gaatatttgc ccaatgacct catcgtcctc gccggcctgc tccaactcga gcaaaccgtc
     8521 ttcgagggac tgaagttgct cttctgcttc tatcaaaaac accttgatgt catcggccgt
     8581 catgcccgga aattccagca tcggactgcc ccctatacgc tctcggccat tgcctcgctg
     8641 atgaactcct gttcgtcttt ggaaagcacg tagtcgaggt cgagcaatat cacgagctcg
     8701 tcctcgagtt tcgctatgcc ccgcagatac ctcgaatcaa caccggaaag aacaggagaa
     8761 ggcggttcga ttatcttcgt gggtatgcga agcacctggg agactgcatc cacaatcatc
     8821 cctatcggct cctctcccat ctgcacaacg actatcctcg tagacttcgt gtgctccgca
     8881 ggaggaagcc caaacctcgt cctgaggtcg actacgggta taacccgccc gcgaagattg
     8941 atgacgccaa ctaccgattc cggcgcatga ggtatctccg ttatctcctg catcctgttg
     9001 atgtcctgca cccgggagat ctccacgcca taggattcat cagccagctc gaagactaca
     9061 agctgaatct cttctgcatc gaatccatgt tccacttgtg acgcccccct atctcagaaa
     9121 ttgcagcaag cctgcctgca ccgatttcga agctgccgac agagcagcat aataaactat
     9181 ctcctgctca cacaggcgca ttatagcctc tgcgaaatcg atatcctcag tcttgctgag
     9241 aaggccttca atagatatgg aatcactctc aagcctgtcc attacgagtt caaggcgctt
     9301 gccctttccg ccgatctcgg ccctatgctt gagaatgttg tcaactactg catccacacg
     9361 cccaagatcc tcttccgaca ggctctccac atcgttttcc cggaggtgcc tttcaatgtc
     9421 gatcatggca tcgaaaacat tggcgccgtc cgccatgaaa acttcatccc cggcaaggct
     9481 tccagagatc acaatcccgg ggcctatctc tattgcgtcc ttgccggaat cgccgtggta
     9541 caacacgcca gtcaccctgc catccgcgtc ccgctccacc ttgaaggggg cctgtccaac
     9601 cttgtgcccg gcgaacatat gctccccatt gtgcatctcg tttcctgcgt tgacaacctc
     9661 ctcgaacagc tccctgacct cgacagctat cccgtcccgt tccgtcggag tcacagtatc
     9721 gctggcggcg cgccccacaa gatccctaag gcggttcata atactgccta ccttgtcaag
     9781 aacgccgtca taagccctga gcttcgtatc cgcgatgtct gcattggtct tgtactgttc
     9841 gagccctttg agggtagacc taagccttac agcctttgca gcgccgtgcg ggtcgtcaga
     9901 aagcctgttt atcgagcagc ccgatgccag ctgctcctga cgcttcacat atctttcaaa
     9961 ggccacattc acattgcgca tggcccgctc catcatgaca ttgttcgtaa cccgcatggc
    10021 cctaacaccc tttctcatct atgtgctgcg ctcaaacccg cgccagaggg cctgtaccac
    10081 tctgcgcccg cctctacctt cccacaagcc ccgttccgtt gatgagcctg tcgagcatct
    10141 cgtctaccgc agtcaccaac cgagcagccg cgtcaaacgc tgatgagtac tttatgagca
    10201 tgatcatctc ttcatccagc gaaactccgg ataccgattc ctgcctcgct ttttgctgct
    10261 ccagcacacc ttcgtagacc tctcgcgacc tcatcgattc ctgggtctca agcccgaggc
    10321 ctgtcagaat cgaaccataa aagtcgcgga acgtagccgt tcccccaacc atagtgagcc
    10381 catccttgag ctgagccatg gccaacgcgt tcctgccatt gccggggctg tcagtctgat
    10441 ctgccgccgc aatcaacccc ggattcccct ctatctcttc tgaaagacga atccccgccg
    10501 cgccctctcc ttcaaaaaag acgtccccgg agtttccaaa gaggtcatac ccggccatgt
    10561 gctgctcatt gattttctca atgagcgcat tggcaagatc gtctagttcc ttgagatatc
    10621 cggggattat catgttggcc gcctcattga ggccctttat ccttccaccc gtaatcaggg
    10681 cgtcttcagc caattcccta gcatcagtcc cggccaccag gatcctgccc ccgacagtaa
    10741 catgcacgag cccgctattg tcttccacaa ccgcaacgtc gacatcggac gcaagctgct
    10801 gaactagata atcccgtctg tccagcaagt ccgctacgtt ttcgccgact acatttcccc
    10861 tgacaatctc ccggttgagc tccgcaatgc tggaagcggt cgtgttgact cgatgtacaa
    10921 gcgacttgat attctgatca atgtctgctt tcgtctcatt gagctgtgcg gcagccaagt
    10981 tgaaggcgga cgccagcgaa atagcctcct gctgaacact ggtccgcctc cccggatcag
    11041 tggcattgcc cgaaagctcc tgccatgata tccagaaccg gtccatcatc gccgagatgc
    11101 caatatcgct cggctccttg aatacttcct ggatctcctc gagaatctcc tggcgcgctt
    11161 cccacctgcc caaagatgcc agggtatgcc ttatctgcgt gtccacgaat tggtcgcgca
    11221 tccgctcaat cccggccacc ttcacccctg ttccgggctg aagcgcccct ggcgcggggg
    11281 gagtcgcctc aatgaccggc ctctgtcttc tgtagcccgg cgtgttcacg ttagagacat
    11341 tgtgcccggt tatgtctatc gccgcccgct gggcgtagag agccctgcgc gcagtctcca
    11401 gtccaacgaa tgtagagtac acttgagctt caccccatct gatacagcta agccgtatcg
    11461 tccactgccg aagacacagc cttcttgtcc atgcgtccca agcggctgta gaccgaattg
    11521 gtcccatccg cctcagctat cagcctgacg gtattctcta tatattcgat tgcatcttgg
    11581 atgagcattg cattgattgc attcgccgag tctatctccg ccaggatgcc ggcgatcgaa
    11641 tcccgaagga cctcgaactc cgccatctcc tcttccttga acaatgcaat catgacagcg
    11701 agagcttgtt cagcatcttc aacttcgact tcggtctcgt ctgcccaacg ctttgccaga
    11761 tcagcaagac cgatctcctc atccctgatg gctcctatta tctcttccgc cctcgatact
    11821 agctcagcca gggccgcaac atcccctgac gtgataacgc tgcgctgcgc cctggccacg
    11881 tagagcaact ccgaacatag cgcaaactcg cgattaagaa tcctcgccat ttcctttgcg
    11941 taatccaccc aggtcacctc gtctcacagc cccggcaaag agcttgcgag caacatcttt
    12001 gccgctgacc tcgtatgtgc ccgattcaat gcgggaacga acagcctcaa ccttgtcgtc
    12061 ccgaacatcg gacacggaag acagagcctg catggcgtgg tgaatctcct tgcccctatc
    12121 cgagatctca acctcatcgg gcttcacctc gcccgttcgt tctgcccttt cagccttctt
    12181 tgccagttgg gagtataggt tgatccgatc aaatccccgg cttccggata ttttcaaggc
    12241 catcacctct cttgagtcct aacttatgta tcggcatggc cccgccctta ctttagccca
    12301 tagaccaccg aagcttcact cgcgaccctt tctcctgcgt tcaggaacgc tcatccgcgc
    12361 cccggaagct ctttccccta attgtgccct cttataacga ctcttttcac catcatcgcc
    12421 ccgaccaagg cctgtttgcc ctcccgggca gcccctgccc gaaaaggcct tcccgaactt
    12481 gtccaagcac ttggcgcagt acacgcccga ctctatactc gccccacaag cccggcagaa
    12541 cagtaccggc cgaacgtcgc tagccatctc tatccgccca tcccttagaa ggtccagcac
    12601 aaatgcctcg tccacaccag tcgcatccgc aatttcagtc aaggttgcgc cggaatgcct
    12661 tctcaggtac tccactacct tctcagcaag cttttccgct tccacaaggc aatccgggca
    12721 aagcggcttc gaaatccgat ggaacagctt cccgcagcgg ctacagttct ccagcgccac
    12781 cctgcttcac cgccttgcca tcgcctgtca tgttcctctc catgaacttg gcaagcccga
    12841 ttccgccgcc cttggcaacc tcctgtgcaa gctgggagtc gagcatctcc ctgaccagtt
    12901 cctcctcttc agatgagaag agtccgccct tgggaatgct agcccgcgcc gacttgagaa
    12961 tcatctcgat aaaaatcgcc tcaaactcgg aacaggcctg cgcgagcctc tcgttttccc
    13021 gggctctagc gccagcctca gctgcggcat cagaccctcc ggccgtctcg agctcgacac
    13081 tcttcgccaa gttcctgcga tcgccggccc ccgcagcaag gccgtacggg agcgagagac
    13141 gcatcatagt tccagccgtc cccaaatcca tgccaaccgg cgaaacatcg cccatcgctg
    13201 cctgcacctc ccacgctcac attatctgca gttcagcctg gagagcgccg cacgccctca
    13261 aagcttccat tatattcatg atatccgtcg gcctcgcccc tacggcattg agcgcggcag
    13321 ccaacgcttc aatggtactt ccagcctcaa gcgtgatcgt gctgcctggc tgtgcaggca
    13381 tggccaccac gtttccgctt gacgcacccg ccccgtcctg ccatccgggt aagcctccta
    13441 cactcacatt gaacccgttg tatgagacag ctgcggcgct caccttcacg ttgccgccta
    13501 ttaccaccac gcctgtgcgc gcattcaata caaccttggc aggagcatca ggcgccacct
    13561 cgagctcgcc aacttcggcg atgaacggcg tcggagtctc ctgaagccat tctggaacac
    13621 tcaccctgac cgttcctcca tcaactgcga cagccgctcc ctgcccgaac ctatcgcata
    13681 ctgcccgtgc tatcctggct gcagtagtgt aatccgggtt gagaagcgac agcaccagat
    13741 ggccatcggg agtgagcatt tccatgggaa catgtctttc gactgtagcc cctgcaggca
    13801 ccctgcccac agtcgccttg gcctcggagc cagtcacaac cggaccctga gccacagcat
    13861 atacccgtcc atcggcaccg gccaattccg taaggagcag cgtcccgcct gcaaggctcc
    13921 tggcatctcc taccgatgac actgcgacat ctatggcgtc acccgggctt gcaaaagcgg
    13981 gcaaaactcc agtgaccatt accgccgcga cattcttcgc cttgatctcc agagggtcga
    14041 cggtgatctt gaacttgtcc aacatgttcg atagcgttct cgcggtgaac ggcgtgttcc
    14101 gggagtctcc ggtacctttg agccctacca caagtccgta ccctatgagt tggttatctc
    14161 tgaccccgta cactctggta atatccttta ccctaacagt tgcgccatct ttccccgccg
    14221 agccagtcag cgcaggttga ctactccctt caccctggct agcctgagac tccacaacct
    14281 ccgtcggctg cgcgccgtag accctgggga tagaacatga cataagcagc aacgcgagcc
    14341 acgccgcaat cttgaacagg cctgatcttc gtcttcgcaa aaccctcacc cccaaggtca
    14401 gaaaatcgta ttccacaccc aaaccaggcc atcccagagc gtcttaaaga atccgccctt
    14461 ctgcccagtg tacaccccgc cgctcatgct tatctgcgcg ttcgccacca tagtcgaagg
    14521 aatagtattg ttcggcccta tatcctgtgg cctgacaacg ccttcaacga ccatgatctc
    14581 cttctcgcgg ttgactgaga cctccctcga accacgtatt actaggtccc cattgggaag
    14641 aacctcaata acctccgcag tcatggtccc aaccagcctg cccgaacgcg tagtctcatt
    14701 cgctcccttt gatgaatcac ccacatccaa cctagcggca gggataaagt cgaatacccc
    14761 tacacctggt tgagcagtga ccccgatgcc ctgtgccgta ttggtctggc tcctgttctg
    14821 cgcatacgag acttcagcaa cgagtatggt caccaagtcc cccacctgcc tggcctttcg
    14881 atccgcatac atcgacgcaa aaggagacgc gtcttcactg taaagagacg tagcccgaac
    14941 gacgcctcct gaaaagacaa gcaccg
//
LOCUS       DAOSYL010000058        14947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3369_length_14947_cov_8.007252, whole genome shotgun sequence.
ACCESSION   DAOSYL010000058 DAOSYL010000000
VERSION     DAOSYL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14947
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3369_length_14947_cov_8.007252"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1147
                     /locus_tag="PLU15_08765"
     CDS             197..1147
                     /locus_tag="PLU15_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOO31052.1"
                     /translation="MKVGDIEKIGVAGAGTMGSGIAQVFASKGYEVVVTDIAEEQLRR
                     SAKLVDIFNSSLIEEGIMLPEEAQKVKGLISYSTDKRDFSDADLIIEAIVEKMNIKQD
                     FWHETEAIAKEDAFFATNTSGLSINGICEKVENKERFIGMHFWNPSHIIPLVELTKAD
                     ETSDETVEELKKLMATIEKEAVVVQKDAPGFIGNRLQFAVFREALYIVDEGIATFEDV
                     DKAMKYGPGFRYPVIGPLQTADLGGLDTFYYISDYLFEELSDMKKPPAMLQSMMDAGR
                     LGVKSNKGFWDYSGDKQAEAIKTRDKMLFDMLKWSKKVRK"
     gene            1358..1562
                     /locus_tag="PLU15_08770"
                     /pseudo
     CDS             1358..1562
                     /locus_tag="PLU15_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589169.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     assembly_gap    1563..1662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1808..2266)
                     /gene="bcp"
                     /locus_tag="PLU15_08775"
     CDS             complement(1808..2266)
                     /gene="bcp"
                     /locus_tag="PLU15_08775"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOO31053.1"
                     /translation="MAKLEIGQPAPDFALPNQDGKMVRLSDFRGRKLVAYFYPRDNTP
                     GCTREAEGFRDLHDQIAAAGAEVVGVSKDSVSSHAKFHSKLQLPFDLLSDPNHSVLEA
                     YGAWGTRTMYGKTSEGTIRSTVIVDEEGRVAAVFPKVKPDGHAQEVLKTI"
     gene            complement(2670..3098)
                     /locus_tag="PLU15_08780"
     CDS             complement(2670..3098)
                     /locus_tag="PLU15_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31054.1"
                     /translation="MDRKDVLTRILAILGTVLVWTPILFTVLTSIVGTISSRVFRFDY
                     LMPAELFPIAFVGALLLLWAARRAKSHQKLIGWGLGGSVAFLAGGQLYAVITGLASGV
                     TEPAGCVWAVALASIALYSLALVMTGIAGMSLVRWLYLSQ"
     assembly_gap    3542..3641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4267..5499
                     /locus_tag="PLU15_08785"
     CDS             4267..5499
                     /locus_tag="PLU15_08785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO31055.1"
                     /translation="MKNPKYVELLITTKLEGIDVVPQAVYFLSFAAIGVPLCINTMLT
                     RPLPEPATSVLLLVLGLISYHVPVTFPSNVQFHPGFPLLMGALYCHGCVAANLVIMPS
                     TLLHFFTRNHGLFNCFFNVGQFALCICAAETVGFHLGWEPGAPATATDLLPISLMMLT
                     FDLLNVLFVSVSRSIENKEPWKKCFIKLLYTDRKAVLPQRTFLTVVSMLLSSRMGDIA
                     FVIVFIGVISLRFQNAFQRELLEKTEEAETDLLTGVYNIRYLQKWLNTQCKAATGAEG
                     VCSFIFADVDGLKTINDEYGHEIGNKLLIHVARLLLANVRSKDRVVRYGGDEFVIAFP
                     GIGLPQATAAAMRILQASDSAPFSVNGAEVDFGISIGVASWPEHGETVFDVIRIADRA
                     MYVAKRSGGNRVQSAADL"
     gene            complement(5944..6501)
                     /locus_tag="PLU15_08790"
     CDS             complement(5944..6501)
                     /locus_tag="PLU15_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO31056.1"
                     /translation="MNETLEVIKRRRSIRNYRPDQITDSELEAILEAAISAPSAMNQQ
                     KWHFTVIQNQDVLKRITDLAKEVMKSGPEPMAKRAAEPDFSPFFHAPTVIIVTGDSAG
                     RFVQIDCSLAAQNIVLAAESLDIGSCIMTSPEIIFKSQEGRELMKELGVPEGYAHVCT
                     VALGYKDGDSPPAKPRRKDVIDYIR"
     assembly_gap    6838..6937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6938..7179
                     /locus_tag="PLU15_08795"
     CDS             <6938..7179
                     /locus_tag="PLU15_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31057.1"
                     /translation="DRMLECLEIYLDEDENGICQSVLDVELYQLKIKIEIEGFEGSWC
                     RRPSPSGIYTILSRQSSISRTITCTCLKSEGGGRR"
     gene            7299..7577
                     /locus_tag="PLU15_08800"
     CDS             7299..7577
                     /locus_tag="PLU15_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31058.1"
                     /translation="MYEYDFGDSWQHTTTLEKTVNSDTLEVTYLDGRGERPSEDVGGS
                     WGYMEYMRIMADETDPEHETLGSKPSPEEINSRLKHCIMGYMYLRSWH"
     gene            8142..9476
                     /locus_tag="PLU15_08805"
     CDS             8142..9476
                     /locus_tag="PLU15_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO31059.1"
                     /translation="MYKFPKGLYVDVRIEETFDTKISFKKLTLQEQKIRNNRGAFIRV
                     FDGRRWYYSSTTDINGIQAQIDALAQMATPNREILEHPIVKAFEVNQETIMEYDAKSV
                     TDIPAQEKQELLESYLTLITDPTIVHHSSFYADNKTVKSIYTSKGTAVTFDKQTCGVR
                     INLELSCGEKKNQTSISKAGVFFEDLLNLEDFFQTELEKNIAFTRDAELVIPGEYPVL
                     LGPEATGVFTHESFGHKSESDFMVGDETMKAEWALGKRIGQSLLTIIDDGTVPGNGYV
                     PYDDEGTKGKKTYIITEGILSGRLHSAATAAQLEESLTGNARAMNFEFEPIVRMTTTY
                     IERGNRPLGDIVTEMENGIYVDTVKHGSGMSTFTLAPDRAYRIENGQIAGPVNVSVVT
                     GNVFSALAEVDAVSEEFELMSFVGGGCGKMEQWPLPVGFGGPYVRVRKLKVQ"
     gene            9490..10722
                     /locus_tag="PLU15_08810"
     CDS             9490..10722
                     /locus_tag="PLU15_08810"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOO31060.1"
                     /translation="MKQESITIREKETAAKIQNTHINAVRTKDITKKGVRVFADGNIG
                     ISGAIGDVAEEKLVENAVQNLSVGIEYPYPISRDRKDHRSYNDNPMTAGELLSITESI
                     LETLRKEYPDFDFSETISANEIMTEMRNSEGLDLKYEDAHYSLGLILKEKKSANLFDG
                     ILMCVGCRFDMDKFWAFNRPYLEAYRNRVALPEGEVLPVFTMEPDNLMSFLNRSLNGE
                     RFATGSSIFSGKMGEQLFNEKVTLEQNRNPLHQPGAFFDMEGVVLPEDRHVLIESGKL
                     VSVFTDKKNAHLYNLPHTGSASGAYDGMPSLTTVPLRFRTDSKDLKGALKGQPAILTV
                     ISSGGDFTPDGSFAAPVQVGFLFDGERIIGRLPEFTMRSHISKMLGEDYIGTFDNTVF
                     YIGDLPTQVQGYYMTIMK"
     gene            10830..11711
                     /locus_tag="PLU15_08815"
     CDS             10830..11711
                     /locus_tag="PLU15_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOO31061.1"
                     /translation="MRVAVILNPNARHGRAAEDEAEAVRTLADLGVDFDLVKTSGPGD
                     ATSMAQSAAEQGYDVVCAMGGDGTVNEVINGIARTGAALGVIPVGTGNDFARTLGVPR
                     GDVAAACRILARGQRRPMDLCRMNDRYFASTFGAGFDARVCKAASERFKRFGGIWTYL
                     FAIMSVIWSFRPVPMRIVADGREISKSPLLVAVTNGKSCGGGMHICPDAEIDDGLFDV
                     CIVDNVPILKFLYSFPRVIRGTHVTMPEVEMLRARSVHISCSRMETYHVDGEVFEADD
                     MELALVPGGIDVVVGEV"
     gene            11715..12275
                     /locus_tag="PLU15_08820"
     CDS             11715..12275
                     /locus_tag="PLU15_08820"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOO31062.1"
                     /translation="MRLLLAAIIGYCVGSISSAVIVGRAVRGIDVRKHGSGTAGAANV
                     GSLLGLGYGVLVACCDIAKGMIAAIAGRAIGGVTGQMAAGAAAVIGHILPVWFGFAGG
                     KAIATFFGSCVLTAPVVVLLAGVLWLVLYLVLRRVAQASAIASACLPLIGWVCRLPWQ
                     HILYLGVMGAAICLRHIPDACAPGEG"
     gene            complement(12546..14294)
                     /locus_tag="PLU15_08825"
     CDS             complement(12546..14294)
                     /locus_tag="PLU15_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31063.1"
                     /translation="MRHITRLVVISRDYWRWMVIALVAMIGVTAANLAGPWLIRSLVG
                     TIEENFGHGTAAAGQVVNISIMLLLVYAVRPALRALQVWATHVAGWGSVAAARKEIYE
                     HLQRLSPKYYSSTQTGQIMSRAINDTAYFESLIAHVIPETIVSLLTVAGVFVVLFSIN
                     PTLALYTLAPIPLIILGFVAYNRYVRPLFRHAQAKLGDLNAVLQDNLSGMREIQAFTQ
                     EEREMESVGKRIMVHSSAITKAVSTSACFHGGIDFFAGLGTVSVVLFGGLMAVQGQVS
                     IADITGFLLYVGLFYEPIMGLNRLNESLQQALAASDRYFEVLDTQPDIDDAPDAVEFG
                     RVEGCITYENVSFNYDETPVLKNINLTIKPGEMIALVGPTGVGKTTMANLIPRFYDPT
                     RGRVLIDGVDIRTATISSLRRNVGMVLQDVFLFNGTVAENIAYGSPKATLEEIIEASI
                     VAGADEFIREMPNGYDTHIGERGVRLSGGQKQRLAIARAILYDAPILILDEATSSVDT
                     ETEAKIAAALDRLMAGRTTIVIAHRLSTIRHADRIIVLDEGEIVEEGTHEELMELGGL
                     YATLVMADASTAGLAS"
BASE COUNT         3562 a         3517 c         4074 g         3494 t
ORIGIN      
        1 tcttgattgg gttgccgatt ttctatttca tgcaaggatt cagcttttgc cggacagtaa
       61 atcgcaccta tggagaatgt gatgatcagc ttatgggggt atgcgggaga aagcttcttg
      121 actaatgcta tcgtcagaat cggctgctat aggcttgcct tcaacgagag atgtgacgac
      181 gaggagagtg attgtaatga aggtcggaga catagagaaa attggcgttg ccggagccgg
      241 caccatgggc tcgggaatag cgcaagtttt cgctagtaaa ggctacgagg tagtcgtaac
      301 ggatatagcc gaagaacagc tgcgaaggtc ggccaaactt gtagacattt tcaacagcag
      361 tcttatcgaa gaagggataa tgcttcccga agaggcgcaa aaagttaagg gactcatatc
      421 ttatagtaca gataaacggg atttcagcga cgcggacctc attatcgaag ccatcgttga
      481 aaagatgaac ataaagcaag acttttggca tgaaacagaa gcgattgcga aagaagatgc
      541 tttctttgct acgaatacgt cagggctcag tataaacggc atatgcgaaa aggttgagaa
      601 caaggaaaga ttcattggca tgcacttttg gaacccgtcc cacataatcc cattggtcga
      661 actcaccaag gcagatgaaa catctgacga gactgtcgag gaactgaaaa aactcatggc
      721 gaccatagaa aaggaagctg tcgtcgtgca aaaagacgcc cccggtttca tagggaatag
      781 gctccagttt gcagtcttca gagaggcttt atacatagtg gatgaaggga tcgccacctt
      841 cgaagacgtt gataaggcca tgaaatatgg gccgggcttc aggtatccgg taatagggcc
      901 tttgcagact gcggatttag gaggactaga tactttctat tacatatctg attacctttt
      961 tgaagaattg agcgacatga agaaaccgcc ggcgatgctt cagtcaatga tggatgcggg
     1021 acgattgggg gtcaagtcaa acaaggggtt ttgggactac tcaggtgaca aacaggcaga
     1081 ggccataaag acaagggata aaatgctgtt tgacatgttg aagtggagca aaaaggtgcg
     1141 gaaataggtt agcagtctac aattttgccc acctagggcg tatttgttca agaagatttg
     1201 cgggtgggca ttattatctc tctggtgctg ctcatgtttc cagcctagag aggtcattcc
     1261 tgttcccatt gtcgttgcta caggcgttga ggacgcctac atgactggat acgccgcggt
     1321 gccatcttcg gccaattaat ggactatgct ggagaggctc accagaatat ctgggcttga
     1381 aatagccagc tgaggagtga tcatcagcag cgctgtgctt acatatgacg aaatcagcct
     1441 gtttgtggtc gtatttgcca tgtatacgct ggcacttgcg ctgtttgggg aggctaacat
     1501 ctcaaggact ctaatcttat gttcaattgg actgggctca ttcacgttta ccatgaccgc
     1561 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccgcg gtaataggcc
     1681 tggtggccga tggtttaggg ttagcaccgt agtctacgtt ggctttgaaa cagagttctc
     1741 tgtcagacga cggcgtggac agctgatttg acactgtttc ggacaggggc aggcaacgtg
     1801 tggctgctca aatagtcttc agcacttctt gggcgtggcc gtcaggtttc accttgggaa
     1861 agacggcggc tacgcggcct tcctcgtcta ctatcacagt ggaccgtatt gtaccctcag
     1921 atgtcttccc atacatcgtc ctcgtgcccc aggccccgta tgcttcgaga acgctgtgat
     1981 ttggatcgct tagaagatcg aagggcagct gtagcttgct gtggaatttg gcatgggatg
     2041 acaccgagtc tttgctgacc ccgacaactt cagcgccggc cgcggcgatc tggtcgtgaa
     2101 ggtcgcggaa gccctcggct tcacgggtgc acccgggtgt attgtctctt ggatagaagt
     2161 atgcgacgag tttacggcct cgaaagtccg agaggcggac catctttccg tcttggtttg
     2221 ggagcgcgaa gtctggagcg ggttgtccta tttccagttt ggccatgcat gaactgcctc
     2281 ccttcatgtt ctgactgtag tgtagcagag tccggacatg cagggcaatc gcctgattta
     2341 ccttgcactt gccgcagtcc aggtttgacg aagccgaggg agtttttgtg agccgtgtag
     2401 gtttgctgac gcgctcagca atctgacccg aagccccccg gtcgtcaccg tgaacaattc
     2461 tcatatggca tctgcgtgat aggcgaatct gttgcgcctg tggacaatac ttgaacgttg
     2521 cccaaggcag ctcagttgac cgggtggagg tggtttggct tgggatggat tctggcaaga
     2581 aacagatata gcaggagtgc ggtgaaaaaa agcagcctag aaagaaattg ggctcaagcg
     2641 gtagttgcag ataaccctgt cttcggatgc tactgggaaa gatacagcca tctcaccaga
     2701 gacatgccgg ctatacctgt cataacaagc gctagcgaat agagggcgat tgaagcgagg
     2761 gcaactgccc aaacgcatcc cgccggctca gttacgcccg aggcgagccc tgtaatgacg
     2821 gcatagagtt ggccgccggc aagaaacgcc acagagccgc ctaaacccca accgatcagc
     2881 ttctgatgag acttcgcccg cctcgcagcc cacaacagca gcagggcgcc gacgaaggca
     2941 atgggaaaaa gctcggctgg catcagatag tcgaaccgga atacgcggga agatatcgtt
     3001 ccaactatgg acgtgaggac cgtgaacaag atgggggtcc acacgagcac cgtccctaag
     3061 atggccagta ttctggtgag cacatccttg cgatccattt gtggtccctc cctctagggc
     3121 ttcaaggttc cattgcaggc gcctttctac cttctattat acacatgccg cgaagccaaa
     3181 gacgaagaag ggccgaaaag aggctaagga gcgccctcat caatgaggaa gcatttggga
     3241 cgttggccag tccacagcgg gattgagatt gagaaatgcg ccggcgagtt gatttcggtg
     3301 cgcgttgtgc ctgagaacgg ttgcagacat gtaagatcat gaagacggga tgagatggtg
     3361 ctgcagacac tgtgtatcaa ggacgtagcc gcgggggcac attcatcttg agaatcatgc
     3421 aaggcaacaa gacttgtttg ttcaggtctc gcttcacggg atgattctgc gcgtggcgcg
     3481 tgtttcttct cacagtgtgt ctgtccaatc tgtgcggtgc ctttgcggaa cgaggtccat
     3541 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntacgagccg atgcaaagac
     3661 gagtatggaa aacgccgtaa gctgcctgca acgccagagc aagcgcacaa tacttaatgc
     3721 cgtgcctgtg cagcttctga cgaatgtcct gcgccatctc tcaaaactgc gaaaatggca
     3781 atgcaaaccg tgcgctcctg ccaattgctc ctatgtcagc acttgaaata ttccaatgat
     3841 agtaatacga taaccgtaac gaaagtcact atcattggag aaaaacacta ttcttcccaa
     3901 tacacaacaa tctgctgcaa aaaagtgagc ctttggcatg tcgagtggcc tgaggcaagc
     3961 acgcaatacc ggattgaaca tctcccttag cggggactgg tttgtgttcg gacgtgctag
     4021 ttgtcggtcg agactgcttt aatcacagcc gtgatgatca ctacagtgct taccaaaacc
     4081 ctgctgtaaa atctatcagt cacaggtgag cctctcaagc gggattaatc gatgcctcaa
     4141 gtctgagtca gagtttgctt accaatagca tgttcgcata aaaaaggagg gacaagaggt
     4201 tcgaatcgtt cggcccatcg gctcccgttc cttcgggccg tggatgatcc aggccatatt
     4261 cagaacgtga aaaatcccaa atatgttgag cttctcatca caaccaaact tgagggcatc
     4321 gacgtagtgc ctcaggctgt ctactttctg tcctttgcgg ctattggtgt gcctttgtgt
     4381 atcaatacaa tgctgaccag gcccttgccc gaaccagcaa cctccgtgtt gctgttggtt
     4441 cttgggctta tctcgtacca cgtcccagtc acatttccgt caaacgtcca gtttcacccg
     4501 ggctttcccc ttctcatggg cgcgctttat tgccacggtt gtgtcgcggc aaacctcgtg
     4561 ataatgccct cgacgctgct tcactttttc accagaaatc atggactctt taactgcttt
     4621 ttcaatgtag gccaattcgc tttgtgcatc tgtgctgcgg aaaccgtggg tttccacttg
     4681 ggctgggaac caggtgcgcc cgcgacagcc acggatctct tgccaatatc tttgatgatg
     4741 ctgacatttg accttcttaa cgtcctgttc gtcagcgtat caagaagcat agaaaacaag
     4801 gaaccttgga agaaatgctt tatcaagttg ctgtacacag acaggaaagc tgtattgccc
     4861 caacgtactt tcttgactgt tgtttccatg ctgctctcat cccgcatggg agacattgca
     4921 tttgtcatcg tgttcatagg tgtaatatct ctcaggtttc agaatgcatt ccaaagagaa
     4981 ttattggaaa agaccgagga ggcagagacc gatctgctta ccggagtcta taacattcgc
     5041 tacttacaaa aatggctcaa cacccagtgt aaggcggcaa ccggcgctga gggtgtctgt
     5101 tccttcatat tcgcagacgt tgatggactg aagactataa atgatgagta tggacatgag
     5161 atagggaaca aacttctcat ccatgtggcg aggctccttc tcgccaacgt ccgaagcaag
     5221 gatcgggttg taagatatgg gggagatgaa ttcgtcatag cctttcccgg tataggcctc
     5281 ccgcaggcta ctgctgctgc catgaggatc ctgcaagctt cagacagcgc accattttcg
     5341 gttaatggcg cagaagtgga ttttggcata agcattggag tggcatcatg gccggaacac
     5401 ggcgaaacag tattcgatgt gatccgcatt gctgacagag cgatgtacgt ggccaaaaga
     5461 agcggaggga acagagtgca atcagctgct gatctgtgat gagactgacc agtttcatgg
     5521 tttgcctggg gcggataggc agtcacagca tccagcccca ggcaaaccag gcaatacata
     5581 ggaagcacca gcattcaaga agagcgaatg ggttccaacg agcctaaccc gagatcgtgt
     5641 gaataggatt gcgctggtgg ttcgaagagg tgagccaggg atgcggtcgc ctcaaacgcc
     5701 agatcgggca agctcttcgg cgatctgaag ggccgcccga tgctggctga ttgtttgcaa
     5761 attataggta gacaacctga aatcaggata ccttcaaata tgacctggca tccatgcgcc
     5821 ttctcacctt gtgtatcctc tttagctgca aggataaggg ttgcgatcct caattccatt
     5881 ttccatgtga tgtccactga gttgcgaatc ggatgccgca acgctggagc gtatggaaat
     5941 ggcctatctt atgtagtcaa tgacatcctt gcgtctaggc tttgctggag ggctatcgcc
     6001 gtccttgtat cccaaagcca ctgtgcatac atgggcatag ccttcaggga cgcccagctc
     6061 cttcattaac tcccggccct cttgcgattt gaagatgatc tcgggggatg tcattatgca
     6121 cgaaccgatg tctagagatt ccgcggccag aacaatgttc tgcgctgcaa gcgagcagtc
     6181 aatctgaacg aaccggcctg ccgaatcgcc agtgacgatt atcaccgtgg gcgcatggaa
     6241 aaagggactg aagtcgggtt cagctgctct tttggccatg ggctcagggc ctgacttcat
     6301 gacttcctta gccaagtcag tgatcctttt gagcacatct tggttctgaa tgacagtaaa
     6361 atgccatttc tgctggttca tggcgctagg agctgagatc gcagcctcaa gaatagcttc
     6421 gagttccgag tcagtgatct gatccggcct gtaattccgg atacttcgtc tccttttgat
     6481 gacttcaaga gtctcgttca tcctttccta accacccttc tctctaactg tcaacagacg
     6541 atttctcttt agctcaacgt ttcaccttcc cacgatactg tcttgcgcaa atcagtcagg
     6601 gttttcggga atggggtatt gcggcatgaa aaaaagccgg gagagtgcgg gttagggtgg
     6661 acaaggaacg taaagtgaac tggtatgcat cagtcatata gccctcaaag gcgggagccg
     6721 aagccaaggg attttgccag aatgaaagac ctcattcggg aggccatcat tgtagcactg
     6781 cgcatggaag gcgtctctga gaccgttatt gaccgataca tggcggatgc aggagagnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg ataggatgct tgagtgtctt
     6961 gaaatctacc tcgacgaaga tgagaacgga atctgccagt cagttctgga cgtagaattg
     7021 tatcagctga agataaagat cgaaatagag gggtttgaag ggtcctggtg ccgtcgacct
     7081 tctccttctg gcatctacac aatcttatcc agacagtctt cgattagcag aactatcacc
     7141 tgcacatgtt tgaagtcgga gggcgggggt cgaaggtgaa gcggattgtg atgaacgatg
     7201 accccgaaac gttggaatgc atggaccctg actcctgcga tatcctgctg gaacggttct
     7261 ctgcgctcaa agacatcttt ccgtaacata gggaaatcat gtacgaatac gattttggcg
     7321 attcgtggca gcacacgaca acacttgaga agacagtaaa ttctgacaca cttgaggtga
     7381 cctatctgga cggaaggggc gaaaggcctt ccgaggacgt gggcggatcc tggggatata
     7441 tggagtacat gcggattatg gcagacgaaa cagatcccga acatgagact ttgggcagca
     7501 agccaagccc ggaagagata aacagcagac tcaagcattg cataatggga tatatgtatt
     7561 tgcggtcatg gcattagggt gtccgaaggg agcgagttgc cgatctggtt gatgagggct
     7621 atcgatattt gcttgacagt aatgtcaaaa aagcctgcag agtctggctg gaagtatggg
     7681 aagtggtaaa gtgcggaagg aaaaacttgg gtctgttcgg cgggttcttc atcagcaacc
     7741 tatgcccaga tccggagttt gaattgcaca acgccgggcg gacgggtttt gcgacttttc
     7801 aggctctatc ccgtatgaca tcggaagtta aggcatcaac tgtgttcgga tacggaacga
     7861 ttgccccaag aatcaggtag gcatctatcc tttgccatat ccgctcattc caaggcatat
     7921 attgccatcg cctccgaaag atgcgtcagg gagcgggttt aatgccagag gatagagcgt
     7981 cagaagcctc gcagttatgg caagtgagat cccgctgggg gacccttcat aatccatttt
     8041 attgcttgca gaacatatac tatcctgtat gatagcgtta tggaaatagt taaacatcta
     8101 gttgaatatt ctaatgccga ggagcaaaaa ggaggcgcgt gatgtataag tttcccaagg
     8161 ggttgtatgt tgatgttcgc atcgaagaga cttttgatac caaaatcagc ttcaagaaac
     8221 tgactttgca ggaacagaag atccgcaata ataggggtgc attcattcgg gtatttgacg
     8281 gcaggcgctg gtactacagc tccacgacgg atatcaacgg catccaggct caaattgatg
     8341 ccttggccca aatggccact ccaaatcgag aaatccttga gcatcctatc gttaaagcct
     8401 ttgaagtaaa ccaagagaca ataatggaat acgatgcgaa atcggtaacc gatatacccg
     8461 cgcaggaaaa gcaagaattg ctcgaaagct acctaaccct cataacagac ccgaccatcg
     8521 tccaccattc gtccttttac gcggacaaca agaccgtcaa gtccatatac acatcaaagg
     8581 gcactgccgt gacatttgac aagcagacct gcggcgtccg catcaacctg gaactatcct
     8641 gcggagagaa gaagaaccag accagcatct ctaaggccgg cgtattcttc gaggatcttt
     8701 tgaaccttga ggacttcttc caaaccgagc tggagaagaa tatcgcattc acaagggacg
     8761 ccgagcttgt aattcccgga gaatatcctg tcttgctcgg cccggaagcc accggggtat
     8821 tcacccatga gagtttcggc cacaagagcg aatccgattt catggtggga gatgaaacca
     8881 tgaaggcgga atgggcccta ggcaagagga tcggccagag cttgcttacc atcatcgacg
     8941 acgggactgt cccgggcaat gggtacgttc cctatgatga cgaaggcacc aaaggcaaga
     9001 aaacatatat catcaccgag ggcattctca gcgggcgctt gcacagtgcg gccacggctg
     9061 cccaattgga agaatccctg accggcaatg ccagggccat gaatttcgag tttgagccaa
     9121 ttgtgcgcat gaccacaact tacatcgaaa gaggcaatag gccccttggg gacatcgtca
     9181 cggagatgga aaacggcatc tatgtggaca cagtcaaaca cgggtccgga atgtccacct
     9241 tcaccctcgc ccctgaccgg gcgtacagga tcgaaaacgg ccagattgcc gggcctgtca
     9301 atgtctcggt tgttaccggc aatgtattca gcgctttggc agaagtagat gcagtgagcg
     9361 aggagttcga gcttatgagc tttgtcggcg gtggctgtgg caagatggaa cagtggcccc
     9421 tgccagtcgg atttggcggc ccatacgttc gggtcagaaa actcaaggtt cagtaagggg
     9481 ggcagagcaa tgaaacaaga atcgatcacc attagggaaa aggaaactgc agcaaagatt
     9541 caaaacaccc atatcaatgc agtacgtacc aaggacatca cgaaaaaggg cgtacgggta
     9601 tttgcagatg ggaatatcgg catatccggg gcaattgggg acgtggctga ggagaagctg
     9661 gttgaaaatg ccgtccagaa cctgagtgtc ggcatcgagt atccttatcc catatcccgg
     9721 gatagaaagg accaccgcag ttacaatgac aaccccatga ccgccgggga actgttgtcc
     9781 atcacagaat ccatcctcga aaccctccgc aaggagtatc cggactttga cttcagcgag
     9841 accatttccg caaatgagat aatgactgag atgcgcaata gcgaaggact tgatcttaag
     9901 tacgaagatg ctcactattc cctggggttg atcctcaagg agaagaagtc tgccaatcta
     9961 ttcgatggca ttttgatgtg tgtgggctgc aggtttgaca tggataagtt ctgggccttc
    10021 aaccgcccct atctggaggc ataccgcaac agggtggcct tgccggaagg ggaagtgctg
    10081 cctgtattca ccatggagcc ggataatctg atgagctttc tgaacaggtc tctcaacggt
    10141 gaacgcttcg ccaccgggag ctctattttc agcggaaaga tgggggaaca gttattcaat
    10201 gaaaaggtta ctttggagca aaacagaaat ccactgcacc aaccgggagc ctttttcgac
    10261 atggaaggcg ttgttctccc ggaggatcgt cacgtcctga ttgaatccgg aaagctggtc
    10321 agcgttttca ctgacaagaa aaacgcccat ctttacaacc tgccgcacac cggttccgct
    10381 tcaggagcct atgacggcat gccttctttg accacagtcc cactgcgctt taggaccgac
    10441 tccaaagatt tgaaaggagc cctgaagggg cagccggcta tcctcacagt gatcagttcc
    10501 ggcggagact tcacccctga tggcagcttt gccgcccccg tccaggtggg cttcctcttt
    10561 gacggtgaga ggataatcgg caggcttccg gaattcacca tgcgctccca cataagcaaa
    10621 atgctgggag aggactacat cggcactttt gacaatactg tcttttacat cggcgacctt
    10681 ccaacccagg ttcagggata ctacatgact ataatgaagt aggggccgtt atggccccct
    10741 ctctcatgcc tgcgcaggaa tgatgattcg attgttgaat cacagaagta tctggatttc
    10801 atcactatgt actgacaggg ggcaggaagt tgcgtgtcgc ggtcatacta aaccctaatg
    10861 cgaggcatgg acgagcagcg gaggatgaag ctgaggcggt gcggactctg gcggacctgg
    10921 gagtcgattt cgatttggtc aagacctctg ggccggggga tgcaacctcc atggcgcaaa
    10981 gcgctgccga acagggttat gatgttgtgt gcgccatggg cggcgatggc acagttaacg
    11041 aagtgatcaa cggaatagca cgcacgggag cggcccttgg ggtgatacca gtgggcaccg
    11101 ggaatgattt tgcacgtact ttaggagtcc cgagggggga tgtcgcagcc gcgtgtcgta
    11161 ttctggcgcg tggccagagg cgtcccatgg acctttgccg catgaacgat aggtactttg
    11221 cctccacttt tggggcgggt ttcgatgcta gagtgtgcaa agcggcgtcc gagaggttca
    11281 agcgtttcgg ggggatatgg acgtacctat tcgcaattat gagcgttata tggtctttcc
    11341 ggccagtgcc tatgcgcatt gtagccgatg gtcgcgagat atccaagtct cccttgcttg
    11401 ttgccgtgac caacgggaaa agctgcgggg gcggaatgca catatgcccc gacgccgaga
    11461 ttgacgacgg gctgtttgat gtctgtattg ttgacaacgt tcctatactc aagtttttgt
    11521 actcctttcc ccgggtgatc cggggcacgc atgtaaccat gccggaggtg gagatgttac
    11581 gggcacggtc ggtacacatc tcgtgttcgc ggatggagac atatcatgtg gacggtgagg
    11641 tgttcgaggc tgatgacatg gagttggcgc tcgtccccgg aggcatcgac gtcgttgttg
    11701 gggaggtgta acctgtgcga ctcctattgg ccgccatcat tggctattgc gtaggttcga
    11761 tatcgtctgc cgtgatcgta ggcagagcgg tgcgaggcat tgacgtacgc aaacatgggt
    11821 ctggcactgc cggagcagcc aatgtcggaa gcctgctagg gctggggtat ggcgtgcttg
    11881 tagcctgttg cgacatagcc aagggcatga tcgccgcaat cgccggccgt gcaatagggg
    11941 gagtgactgg gcagatggca gccggggcgg ccgcggtgat cgggcacatc ctgcctgtct
    12001 ggtttggatt cgcaggaggg aaggcgatag ccacgttctt cggctcatgt gtgctcactg
    12061 caccagtggt agtgctgctc gcgggcgtac tgtggctggt gctctatctt gttttgcgcc
    12121 gagttgccca ggcgtctgcg atagcttcag cgtgcttgcc tctaataggc tgggtgtgcc
    12181 gacttccatg gcagcacata ctgtacctcg gggttatggg agcagccatc tgcctgaggc
    12241 atatcccgga tgcatgtgca ccgggagagg ggtagcgcta tcgtggaggc gttttcaaac
    12301 gagcgatttg gacgcagcgg cgtcttacta gttgatatcg ttgtagaccg cagaagacgt
    12361 gctcgcgact gagaactata tgtaggattc atgtgtcatc agacgataga gaagctcctc
    12421 aaagcatact atagacatat tatggatgtg ctgcattggt tcgcattact gcaagttccg
    12481 ggtcatctac tactcccgca gcatgcgaag acttttgggc caggggttgt gtgctcttgt
    12541 tccattcagg acgcaagccc tgctgtgctg gcgtcagcca tgactaacgt agcgtaaagg
    12601 ccgccgagct ccatcagctc ctcatgggtt ccttcttcca ctatttcgcc ttcatctagc
    12661 acgatgatcc tgtcggcatg gcgaatggtc gagaggcggt gggcgatgac tatcgtggtt
    12721 ctgcccgcca taagcctatc cagggcagct gcgatcttgg cttctgtttc ggtatccact
    12781 gatgatgtgg cttcatccag tatgaggata ggcgcatcgt agaggatggc ccttgctatg
    12841 gcgagcctct gtttctgccc gccggagagc cgtacgcctc gctcgcctat gtgggtgtca
    12901 tacccgttgg gcatctccct aatgaactcg tcagcccctg caactattga agcttcgatg
    12961 atctcctcca aggtagcctt ggggctgccg taagcgatgt tttcggctac tgtcccgtta
    13021 aacaagaaga cgtcctgcaa caccatgcct acattccggc gcagagagga gattgtagcg
    13081 gtgcggatat ctactccatc gatcagaacg cgtcctcttg tgggatcata gaacctcggt
    13141 atcaagttgg ccatggttgt tttccctacg cctgttgggc ctacaagggc aatcatctcg
    13201 ccgggtttga tcgtaaggtt tatgttcttg agtaccgggg tttcgtcata gttgaaggag
    13261 acattttcgt aggttatgca gccttcgacc cttccaaact ccacggcgtc gggggcatcg
    13321 tctatgtcgg gctgtgtgtc gaggacttcg aaatagcggt cggaggcggc caaggcttgt
    13381 tgaagcgatt cattgagccg gttgagcccc atgatgggct cgtaaaagag gcccacatac
    13441 aggagaaacc cggtgatgtc agcgatggat acctggcctt gcaccgccat taggcctccg
    13501 aacaagacga ctgagactgt tccgagacct gcgaagaagt cgattccccc gtggaagcat
    13561 gcgctcgttg aaacggcttt cgtgatggcc gaagaatgca ccatgatgcg ttttcccaca
    13621 gattccatct cgcgttcctc ttgggtgaat gcttggatct cccgcatccc gctgagattg
    13681 tcctggagaa ccgcgttgag gtctcccagc tttgcttggg cgtgacgaaa tagggggcgg
    13741 acgtatcggt tgtaggcgac gaagccgagt atgataaggg gtatgggcgc caaggtatat
    13801 agagccagcg ttggattgat ggagaagagc acaacgaaaa cgcctgcgac tgtgaggagg
    13861 ctgacaatgg tttccggaat cacatgggcg atcaatgatt cgaaataggc tgtatcattg
    13921 atcgccctgg acatgatctg tcctgtctga gtggacgagt agtacttcgg ggacagtctt
    13981 tgcagatgct catagatctc ttttcgcgct gcagccacgc tgccccaccc agcaacgtga
    14041 gtagcccaga cttgaagggc gcgaagtgcg ggcctcacgg catacacgag gagtagcata
    14101 atggatatgt tcactacttg gccagcagct gccgtcccat ggccgaagtt ctcttcaatc
    14161 gttccaacaa ggctccttat cagccagggg cctgctagat tagcggcggt tacgcctatc
    14221 attgcaacga gcgctatcac catccagcgc cagtagtcac gtgatatcac tacgagacgg
    14281 gttatgtgcc gcaatatgta gtcaccccat gtcatcattc tttgtgtgcc cagatatcat
    14341 aatcacgcag cgtgtaagtt gttcaaatcc ttctccaacc cagcggattc tcagagcttg
    14401 tggcattcac attagtgttc tccttctctg gaagagttcc ttcggaacgg cgtttctctt
    14461 catctgcatt ttccttattt gctgcgaaag ggggatgttg ccgtcagtcc ctcatgatga
    14521 acaaagtcgt aggttttgga acgacggcgg ctatgccaaa gaggagctcg aggccaagag
    14581 agatcgcatc agcaggctgc gaagacccag attggatgac gtgttttgga tcgctgcaag
    14641 ttcgatgttg acgatattgc caacatggtt gtgctttttt cagcaggatg aggaatatcg
    14701 ccaggtccaa gcgcccaaag ctgatgtggc atgaagagat aaaacagcga gtgcgggtta
    14761 ttgacggaca agacaaagga gttgttggaa tggataaaga ctcaggctca agcttggcaa
    14821 agggtatttt gaatcgtgga cagaggtgcg taattgacaa ctaactctgg gccaatctct
    14881 tgatcaaata gagaattacg tcgcggtctt ctgggcgtag tttctggaga ttgtgagtga
    14941 ttgcatc
//
LOCUS       DAOSYL010000059        14706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3443_length_14706_cov_7.536960, whole genome shotgun sequence.
ACCESSION   DAOSYL010000059 DAOSYL010000000
VERSION     DAOSYL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3443_length_14706_cov_7.536960"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..1967)
                     /locus_tag="PLU15_08830"
     CDS             complement(351..1967)
                     /locus_tag="PLU15_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO31064.1"
                     /translation="MLRGKVLLAECLAAALILCAAGVICHLGPGKTGVFETQLDSCWR
                     QFGQATVAASSGSGTLSTEGWGFETYVGYGDLLSVSTKAGWENEASGLFEIAGWSSYS
                     DVVAIVRREDLGDERITWLVSEPDGWFSGTVWFVDGPGRYSVSVATRPPKKASYIVVC
                     SFCVHNTSDEIPLAPIEYVGYGHDVTIDSPRQESNVVTDLLNVRGWSRYPRVRATVEN
                     METGEIRTYYTQTAQDGTFEFPVPLDVGYGPCEVILSSCQIDAETWRGAAAYRLISEE
                     PPLYVTQPAYQRGSILATERFKVRGIARGAEAVILETVSYEGDAVLRRQEAQVAEDCW
                     EAWVDLPEGEDLFRLRIGRRLSGSEGENCEVNRSYTVARVKPRDDPAPTELWMTQSVS
                     MAIRELAESIVDGVNDTYERVRAVHDWVAGNIVYDVAGARNGRPGSGDAITTLRRREA
                     VCLGYSNLTAALLRAAGYRVWVVIGSADNGVSVLRHAWNEVEVDGRILSIDATWDAGC
                     IERGVFVRRLSGALFDPSPELLAFSHFAEI"
     gene            2599..3378
                     /locus_tag="PLU15_08835"
     CDS             2599..3378
                     /locus_tag="PLU15_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31065.1"
                     /translation="MRFRRIAAATAIALIFAVTLAGCGGGGQKKLMSYFPDEEGYSWE
                     YEGTDGYVHRMTLDDIYEDSSTKEVTFTITGEIEGIEEEDDDDVDDVWSAFDLEEEEE
                     EEEEDDWSWSWDDEEDDDEDDWGWGSWWDDEEDTAATAGSTTLELRYEFTKDSVYEDI
                     IDGELALPHKFTRLQVLKTPIKKGTKWTYETPDWTEVEAEIIEVGKDDDKLDYVKVQY
                     TAELYEGTYTETRVYKKGLGLVEFTADNPDGSTFSYSVISW"
     gene            complement(3491..5227)
                     /locus_tag="PLU15_08840"
     CDS             complement(3491..5227)
                     /locus_tag="PLU15_08840"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOO31066.1"
                     /translation="MDYAAVYESWRTSSCVDEATKVELAGIENDVAEIEERFGRELGF
                     GTGGMRGKLGAGTSMINRYTVRRVTQAYASYLRECVEGSSEAGVVIAYDSRRMSRELA
                     EEAARVLAAAGIRAMVFDGIRPTPLLSYAVREMDAAGGIMITASHNPPEYNGYKIYGS
                     DGVQLLPEEASQIAARVSGIDDYLCFAAKPVAPVEGIDGEMDERYVSRVTELIEELVG
                     NMVSRRDREGLGVVYSPLHGTGYLLVPRALSDLGFAGLNVVREQSVPDGDFPTVKLPN
                     PEDPSAFELSIDRAMSMDVRNRPAIIIATDPDCDRVGVAVWDGEGYRVLSGNQVGVLL
                     ADFLIESAKMRGIELGECAIVKTIVTTEMVRALADGEGVHVIDTLTGFKYIGAHIDEL
                     EAKGRHFLLGLEESCGYLAGDFVRDKDGVIASALVASVAAWHMAHGRTLLDRLNELYR
                     THGYYYERLVSVPIAGDGGGVEALDALRQEMPDYIAGFPVVEVHDYLTGWTKKRGAGI
                     RVPIDLPRENCIQLVLSRGSKITLRPSGTEPKLKLYVAVRAGSQLKAEAKADAIVTTA
                     MRLLPISQEGYD"
     gene            complement(5312..5881)
                     /locus_tag="PLU15_08845"
     CDS             complement(5312..5881)
                     /locus_tag="PLU15_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31067.1"
                     /translation="MKKGRNAWIVAAVLCVAVVAVLIIGKGMKPDKKEANVENAGPPR
                     EKEVDAVEVMRPTELMTEDGLFLLEVPEGAIFELAADEDDVVWVYGPSGTRYKIRHED
                     VSAILKHRVSMDGYDDAELKQFGFQVAAARVAAVMQSAYPEEGTQLTVGMRTEWQDKV
                     EIEMATKSGISIPQYSLMVEWAVYKGWLD"
     gene            complement(6139..6214)
                     /locus_tag="PLU15_08850"
     tRNA            complement(6139..6214)
                     /locus_tag="PLU15_08850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6179..6181),aa:Arg,seq:cct)
     gene            complement(6236..6311)
                     /locus_tag="PLU15_08855"
     tRNA            complement(6236..6311)
                     /locus_tag="PLU15_08855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6276..6278),aa:Arg,seq:acg)
     gene            complement(6462..10700)
                     /locus_tag="PLU15_08860"
     CDS             complement(6462..10700)
                     /locus_tag="PLU15_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31068.1"
                     /translation="MTEMRAFCCRRRGPSACRAFSLIIFVLASAVLISFMASEAPAYA
                     AEKQEAPLQIGGDVEFKLRSAWGDSAALLGTGYTPGRFDLMQLLSLNVRGQVAPGLTI
                     SANLDNRKDGNLQVMELKLDGDPLKGRFGGLSFRSQTPYTSYSARLRGLELNAKFPEV
                     EMGVTFGRVQGIAAKKTFQGNTAQEVIVYEPAATYGPSPSASGFSASIDGMEYYLLSS
                     SYDPDFMGCWIRYDDEDELGEGRTLEETLNLWDLGYLYLEDPEARGGISAGGKVPLAR
                     GQFITVSSSEEMLALRSEIRDILRGQIQSMIRSYNSEHGLVGSAQKRYPFVYGSETEA
                     AFLDDLISRHAHIIAGMEDGGPGTTVLDARADSYLRRRLYDLGQTDVVPGSVAVEVRK
                     AGKFLSVETELPLIYQVAYDIGVIEFDFPPSFFDIYDAVRIKYRHTVAMGIFNLGLSI
                     AQGSEKVYLNDVLLQKNIDYTIDYELGILTILRTLGPDDVVKVEYEYFRGPFGAVADY
                     KSNFYGAIFGWTPNENLKLKMDVAVYSDDPKSAAMPEATSTMPNTHAIIGFSGQYDNK
                     GLNLSTDLAFSHDQFPFDDNRKTNAANRISGIMGAVDAESKSYLLISHSDGVSVGGDE
                     FRNYSIGSGLASPVVRDVASADENWFFATDGGLTVLSATPGPAGQNPFDYVSNWRRIY
                     MSAGLPSNNLTCVATTPWSVWVGTADKGVACADLADLDTWTVYRKSAGSGLPSDAISD
                     IAYDPAHDLILVGFGDGVAVLEGAHFEHELAGVGVTSIVSATSAEAVDGWRTFAAAKD
                     GVYARDEAGGWTKVVDDSIVKDPAAIEVWNRLLWIGGRNGLWVWDGRNCDIVEATRGY
                     SITALGVGRGRKYEGGETLWAGTAGIPAGTGKDPYRIVVFEVLTPEIVAEHDGTELGV
                     SGHDPRRYVEIAAQDHTTDGYAARATARYSLGSGSIYGNYETLAPGFTKLGQTSRQAM
                     DVWRLGTQWPVGSKANVTAEHSESRTQSHSEKGEQPIGREVLVISNRVGGTIDIGPKI
                     DVSYVLSQVDDNEADGFEREEHTLSITGRQSFFENKLTLGAGYETVQSENLIRPSSSY
                     LQVNLRGDATFKIDALSVSALYRKPVKTVAPGGADQRVTGTEEVSLNAQWGKQLGPIA
                     LKAYYRQTLRDDIATERRFDDKRAEVRATLPVLSFNENALTPSTVLKWEKTVPYSGQA
                     KEGITVQGSLSGNLGSFRTSGGINLKRTEYPGIEKLTLDTEVFATLSSTTRGRFVPQF
                     DLRWKRSSSERAGLGVVASDSVTGTARAIYTPKQGLSNVASLAYSLTSSSGSMGVQKH
                     SVSLQDTIDYRLNEKVTITGEASVRSTAPELGALAGGEGSDIRGALKSGLRYRISDMW
                     SFGASAGYNVYKAPASQNGFAKGLTLEAGLRAVF"
     gene            complement(10851..11744)
                     /locus_tag="PLU15_08865"
     CDS             complement(10851..11744)
                     /locus_tag="PLU15_08865"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOO31069.1"
                     /translation="MRKKLNSWLVMVALAAMAVVALTPAFAVASEDPVGTAAVFDIGM
                     GARALGMGGAFVAVADDANAIYYNPAGLANVEGHKVTSLYSTLYGAGNYIGAGYAQKN
                     VGVGVIGLMSTVTEKDEYGNPGNEFGYREGALMGGYARNFGMFSFGGSLKLYSQTIPN
                     NEGFGVTGDVGALIEMPWMEGFRLGAVARNVGGTAKWQSGYTDQFDPEYVIGASMKPI
                     PKLVVAADFDISNATGHVGAEYKIMPQFAVRAGGVFAEDGDMRFTAGAGFSVQNFQID
                     YAYQGHTALPDSHWLSLGMSF"
     gene            complement(11760..>14706)
                     /locus_tag="PLU15_08870"
     CDS             complement(11760..>14706)
                     /locus_tag="PLU15_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO31070.1"
                     /translation="GGGYSRALEEAIANSGMLFIAAAGNAGIDNDIVPHYPSSYDLPN
                     VIAVAATEWNDKLANFSCYGANSVDIAAPGHMIISCYPDGNQSAWAWMGGTSMATPHV
                     AGAAALVIAEYPHLPHYPGAPGWKPGQETIKDILLKSGDPLPDLVGRTTSGRRLNIYN
                     ALTRTYPPGIEYARADVTFGAPPLAVRFEAGLENPGGVEECWWQFGQENVYGLVANRT
                     LEDEGSGVAWFYAKGLDGSISKMPVQVTAAKPGTIIYVDDTGAFDVGIPVNAFFMWAA
                     EAAGVSYVRVDTRYPLGLSETAVENPIFWDTGFTRFEVVSPFDQEFLASFMDNGGKVF
                     LAAPDYLGDMGLDWFGADYLHIMGAYGVNVPIDIYKGVSGDPITDGIEMEWQVQTGRD
                     DLIAPDLVSSPIMIGAWDLTEDGDEERVIMDLPGAVGLRHANETYRLVYLASPWSSLM
                     YAWDGYDPDDPDINTTPYLLTKIYEYLMGEINIPPVIDNAEASLYFARTGQNIAFVGE
                     AHDPDPIQGGGIAYLWDFGDETRVEEAKATHAYAEPGVYWPTLWVADAEGEVVGAELF
                     VIVLDEEGIVFVDDDESAPDTEEYFKDLFARLEKQFVTVEPSDVIGNGGAKAGLEQFR
                     VVWNCGQYGSLDDAEQAAVADFLDKGGRLFLMGPEVLWDIDPEESEFVRNYLHVVGKE
                     DDVGTEKVRGVAGDPISDGLEIALDFESQGFDDGTDSLELGEGAMPVFINDNGKPCAL
                     RYEGEHRLTFFAFMFEAIPDEVPVAFVSENEEPEPEPLTIPMMLMGTVLDWLGIEPQV
                     EVVAPVNGDEWYGKNDVKWSATHPEDAELTITLAYSFDDGETWTTFASGLENNGEYEW
                     DVSKALKSGPCLIKVIASDCYGNSGEGISGKFVVINAGVNSFVAGPVPASDVVNFYVN
                     ASGGATLYVYDVAGRLVFSEEIAEGEFFYAWPLVDKADKPLANGLYLCFMVTADGVKS
                     DTMRLVISR"
BASE COUNT         3435 a         4574 c         3493 g         3204 t
ORIGIN      
        1 cccgcccgaa catgatattg ccttttcgcc atcctcctcc acagcaatcg cgccatgagc
       61 taccgctgca agcatggtga cgcgatgcag ggcaacgcca aaactgttcc cgtagctttt
      121 ctacccgtca tgtcgcatcc tcccacggag cccatgctgt tcatgcacta tcaggcatat
      181 ttatactgct cagcgcctac taccaaaccc ccgtgcatac actgcgcttg tatctttggc
      241 tgtcgggcat ttactttcca aataacaatt gaagctacaa gttgattaca tcctttttcg
      301 ctccgagcaa caatccgtgc attctccatc caagcactgt gccagtggca ctagatttcg
      361 gcgaaatggc tgaaagccag gagctcaggc gacggatcaa acaatgcccc actcagccgc
      421 ctcacaaaga ctcccctctc tatgcagccg gcgtcccatg ttgcatcgat cgatagaatc
      481 cttccgtcca cctcaacctc attccaagcg tgtcgcaaca cggacacccc attgtcggca
      541 ctgcctatca caacccacac acggtaacct gcagcccgca acaatgccgc cgtaaggttc
      601 gaatatccaa ggcacacagc ttcgcgcctg cgaagcgtgg tgatggcatc cccagagccc
      661 ggccggccat tccgggctcc cgccacgtcg tacacgatgt tcccggcaac ccagtcgtgg
      721 acagctcgca cacgctcgta ggtatcgtta acgccgtcca caattgactc cgcaagctcc
      781 cttatagcca tactcacaga ttgcgtcatc cacaactctg tcggcgccgg atcatcccgc
      841 ggcttcaccc gagccactgt gtatgaacgg ttcacttcgc aattctcgcc ctctgacccg
      901 ctcaaacgac gccctatgcg caacctgaag agatcttcac cttcaggcaa gtctacccat
      961 gcctcccagc aatcctcggc cacctgagcc tcctggcgcc gcaagaccgc atctccttcg
     1021 taggacacgg tctccaaaat cacagcctcg gcgccacgcg ctattccccg gaccttgaac
     1081 ctttcagtcg ccagtatcga ccctcgttgg tatgcgggtt gagtcacgta tagaggaggc
     1141 tcttcggata tcaagcggta tgcggcagcc cctcgccatg tctccgcatc gatctgacag
     1201 gaagacagta tgacctcaca agggccatac ccaacatcga ggggaacggg gaactcgaag
     1261 gttccatcct gcgcggtttg ggtgtagtaa gtccggatct cgccagtctc catgttctcg
     1321 accgtcgccc tgacccgtgg ataccgcgac caaccccgca cattaagaag atcagtcacg
     1381 acgttcgatt cctgcctcgg agaatcgatc gtcacatcgt ggccgtaccc cacatactca
     1441 ataggggcga gcggaatctc gtccgaagtg ttgtgcacgc agaaactgca aacaactatg
     1501 tatgatgcct ttttaggcgg ccgcgtcgca acgctcacgg agtatcttcc aggcccatcc
     1561 acaaaccata cagtccccga aaaccagccg tcaggctcgg aaaccagcca cgtaatccgc
     1621 tcatctccaa gatcctcgcg acgaacaatc gcaactacat ctgaatagct cgaccatccg
     1681 gcaatctcaa aaaggcccga agcctcattt tcccatccgg ctttcgtaga cacgctgagc
     1741 agatccccat accccacata tgtctcaaag ccccatcctt cagtcgacaa cgttccagat
     1801 cccgaggaag cagcaacagt tgcctgtcca aattgacgcc aacatgaatc caactgcgtc
     1861 tcaaagacgc ccgtctttcc gggccctaag tggcagatca ctcctgccgc acacaaaatc
     1921 agcgctgccg cgagacattc cgcaaggaga acttttcccc tgagcatcat cgcaccccct
     1981 gcaacaagcg ctttgccatg tcattttcac cacatatcct atagccatgc cctctcgaac
     2041 catgtaagtt tctatctgcg ggcccagagc gaaattccca tgatttcgac ctgtcttcaa
     2101 gagtaggagg taatttacgg aggttctcgt tcccaggcga taccggcagc gacgaaaatc
     2161 acgaaccttg ccgcaggtcc tgtcgaacgc tgaaggagca tactgtctgg atacgaaaaa
     2221 gggcgcaatc tcgaatgcgc cagcgtagac cgtccatgct gctcccatcc ttctgaaaag
     2281 cagggcgggt ctaccagaaa tgccggatat gtaatataat gtggttagcc catctgccgg
     2341 gcggcggtgg acaaaagtcc cggtccgtgg tatttatata tattgagggg tacttatgcc
     2401 caacttccat ctgtccaggg caactcccgt ccgcctggta gacatagaga cacgaatgac
     2461 tgattagagt gaaaattcgt gatccttcct gtgacggcgc tgcgttaaat caaaacctgg
     2521 aagatggacg ttggtttcca ggccgccagc tggcctatct gggcggcttg agcaaatata
     2581 cgaggagctg ataggtgtat gagattcagg aggattgcag cggctactgc catagccctc
     2641 attttcgcag taacgctagc aggttgtggt gggggcgggc aaaagaagct catgtcctac
     2701 ttccccgatg aagaaggata ctcatgggag tatgaaggca ccgacggata tgtccacagg
     2761 atgactttgg atgacattta tgaggactca tccactaaag aagtcacctt taccatcacc
     2821 ggcgaaatag aaggaatcga agaagaagac gatgatgatg tggacgatgt ctggagcgcc
     2881 ttcgacctcg aagaggaaga agaggaagag gaagaggacg actggagctg gagctgggac
     2941 gacgaagagg atgacgacga ggatgattgg ggttggggca gctggtggga cgacgaagag
     3001 gacactgctg ccacagctgg ttcgaccaca ttggaactga ggtacgagtt cactaaggat
     3061 tcggtctacg aggatatcat cgatggggag ctggcgcttc cgcacaagtt cacgaggttg
     3121 caggttctga agacccccat caagaagggc acaaagtgga cgtatgagac ccctgattgg
     3181 accgaggttg aggcagagat catcgaagtc ggcaaggacg acgacaaact ggactatgtt
     3241 aaggttcagt acacagccga gctgtatgaa ggcacctaca cagagaccag ggtatacaag
     3301 aagggtctag gccttgtgga gttcacggcc gataatcccg acggctccac gttcagctat
     3361 tcggtgataa gctggtaatc taacatacac acagtcaacg tagttcacag ggggcggcat
     3421 atgccgcccc cttgtggttt catttatatt tctttccgaa tgcctcagga agccgagtct
     3481 ggtccctccc tcaatcatag ccctcctgcg atatcggaag gagcctcata gcagtcgtca
     3541 cgatagcgtc agctttcgcc tcagccttca actgcgatcc agcacgaacc gccacgtaca
     3601 atttgagctt gggctcggtg cctgacggcc tcagcgtgat cttgctgccc cgcgaaagca
     3661 caagctgtat gcagttttct ctaggaaggt cgatcggaac cctgatgccc gccccacgtt
     3721 tcttcgtcca accagtgaga taatcgtgaa cctcaaccac agggaatcca gcgatgtagt
     3781 cgggcatctc ctgccgcaaa gcatccaagg cctctactcc gcccccatct ccagcaatag
     3841 gaaccgaaac aaggcgttca taatagtatc catgagtccg gtagagctcg ttcaagcggt
     3901 ccaacagcgt gcggccatgg gccatgtgcc acgcagctac ggatgcaacc agggccgatg
     3961 cgataactcc gtccttgtca cgaacgaagt cgccggcaag atacccgcaa ctctcctcaa
     4021 gcccgagcag aaaatggcgc cctttcgctt caagttcatc tatgtgcgct ccaatatact
     4081 taaacccagt aagggtgtca atcacatgca caccctcgcc gtctgcaagc gcccgcacca
     4141 tctcagtagt gactatcgtc ttcactatgg cgcattcgcc gagttcaatg cctctcatct
     4201 tggcgctttc aatgaggaaa tccgcaagca agacaccgac ctggttccct gaaagaaccc
     4261 gatatccctc gccatcccat actgccacac ccacgcggtc gcaatccggg tcagttgcga
     4321 tgataatcgc agggcggttc cggacatcca tcgacatggc gcggtcaatc gacaattcaa
     4381 aagccgacgg atcttccgga ttcggcaact tcactgttgg aaagtcgccg tcaggcacag
     4441 attgttcgcg taccacgttc aaccccgcaa agcccaggtc tgacaaggcc cgtggaacga
     4501 gcaagtagcc tgtgccatga agagggctgt atacaacacc aagtccttca cggtcgcgcc
     4561 tggaaaccat attccccaca agctcttcga tgagctctgt gacacggctg acataccgct
     4621 catccatttc accgtctata ccctctaccg gagccacagg tttagcagca aagcaaagat
     4681 aatcatctat gccggaaacc ctggctgcga tctggctagc ctcctcagga agcaactgca
     4741 ccccatcaga accatagatc ttgtaaccgt tgtactccgg agggttgtgg cttgctgtta
     4801 tcatgatccc gcctgctgca tccatttcgc gaactgcata ggaaagcaac ggagtaggcc
     4861 taatcccgtc aaacaccatt gccctgatgc cggccgcggc aagcacccgt gcggcctctt
     4921 cagctaactc ccttgacatc ctcctagaat cataagcgat cacgactccg gcttcgctcg
     4981 acccttccac gcattcgcga aggtatgaag cataggcttg agtgacacga cgaaccgtat
     5041 acctgttgat catgctcgtg ccagcgccca gcttgccccg catgcctccg gttccaaagc
     5101 caagttcccg cccaaatctc tcctcgattt cggcgacgtc gttttctatg cctgcaagtt
     5161 ctaccttcgt cgcctcatcg acacagctcg acgtgcgcca gctctcataa accgctgcat
     5221 agtccatcaa taaccacccc atatggctac cttgctcgca tagcgaatgg ttctccgaaa
     5281 agccccgaag ttccttcgac aacttccgac ttcaatcaag ccagcccttg taaactgccc
     5341 actcaaccat caatgaatac tgtgggatgc ttatcccact cttcgtcgcc atttcaattt
     5401 ccaccttgtc ctgccattca gttctcatgc ctacagtaag ctgcgtccct tcttcaggat
     5461 aagcagactg catcaccgcc gccacacgag ccgcagccac ttgaaaaccg aattgcttca
     5521 gttctgcgtc gtcgtaccca tccatagata cccgatgctt gagtatggcc gaaacgtcct
     5581 catgcctaat cttgtatctc gtcccacttg ggccatatac ccataccaca tcgtcctcat
     5641 cagcagccag ttcgaatatg gcgccttcgg gcacctcgag caggaacagg ccgtcctctg
     5701 tcatgagctc cgtagggcgc ataacctcaa cagcatctac ttccttctcc ctcggaggcc
     5761 cagcgttttc cacattcgcc tccttcttat ccggcttcat ccccttgcct atgatcaaca
     5821 cagcaaccac ggcaacgcac aatacggccg ccacaatcca agcatttctt ccttttttca
     5881 taagcatcac tccaccagat agtgctcgcc ttatagaata ttatcacatc ttcgtgctca
     5941 aagaggacaa acttgttccc ctcgtgagag ggtcaaaccg cctgaacccg gcaggaaatt
     6001 gggccatgga aagatctatc cgcaaaagct agcccctcgg caacaatcga aaacattgcc
     6061 aaggcgggca atattggatc acagttgtcc acatacaaaa ataacgcggc agaaagctcc
     6121 accgcgtcgc cataacaatg gtggccctgg caggaatcga acctgcggcc aatggtttag
     6181 gaaaccactg ctctatcccc tgagctacag ggccacacaa acatgctccg aaaaatggcg
     6241 cgcccggcgc gattcgaacg cgcgacctgc ggattcgtag tccgtcactc tatccactga
     6301 gctacgggcg cactcaacgg gcctgccaaa tagcaaggcc acatcgctca cacattatat
     6361 cacgcgcatc ccgcgcaagt caacgatacc cttctccaat ttacagaagc tgccatgcat
     6421 attctccagg caatcagccg catagttggc gtcctctctc cctagaatac tgccctcagc
     6481 cccgcttcca aagtcagccc ttttgcaaac ccgttttgac tggccggagc cttgtataca
     6541 ttatagcccg ctgaggcgcc gaacgaccac atgtcactga ttctgtaacg aagcccactc
     6601 ttgagcgctc ccctgatatc agaaccctcg cctccagcaa gagccccaag ttcgggggcg
     6661 gtcgacctca cagaagcctc ccctgttatc gtaaccttct cgttcaacct gtaatcgata
     6721 gtatcctgaa ggctcacaga atgcttctgc actcccatcg atcctgatga tgatgtgagg
     6781 gagtatgcca gcgatgctac gttgctcagt ccctgtttgg gagtgtagat tgcccgagct
     6841 gttcctgtca ccgaatcgct cgctacaacc cccaaccccg ccctctcaga actcgatcgt
     6901 ttccacctca gatcgaactg gggaacaaac cgcccgcgcg ttgtcgaact gagggtagcg
     6961 aagacctcag tatccaaagt cagcttttcg atgccgggat actcagttcg cttcaagtta
     7021 atcccgcctg atgtccggaa ggagcccaga ttacccgaca ggcttccctg aacagtgatg
     7081 ccctccttgg cctggccgga atagggcaca gtcttctccc acttcaacac cgtgcttgga
     7141 gtcagagcat tctcattgaa cgaaagcact ggcaatgtcg cgcgaacttc agcgcgttta
     7201 tcgtcgaacc ttcgttctgt ggcaatatcg tcgcgcaaag tctgtctgta gtatgccttg
     7261 agcgctattg gaccgagctg tttcccccac tgcgcgttca ggctgacctc ttcagtgcct
     7321 gttacccgtt ggtccgcccc tccgggcgcc acagttttca caggttttcg atacagcgcc
     7381 gacacagaaa gagcatcgat tttgaacgtc gcatcgcccc gcagatttac ctgaagatat
     7441 gagctgcttg gcctgatcaa attctcactt tggaccgtct cgtagccagc acccaatgtc
     7501 agtttgtttt cgaagaagct ctgccgcccc gtaatagaca gagtgtgctc ctcccgttcg
     7561 aacccgtcgg cttcgttgtc gtccacctga gacagaacat atgagacatc tatcttcgga
     7621 ccgatatcta tggttcctcc gactctattc gatataacga gcacttccct gcctatcggt
     7681 tgctcgccct tctctgaatg cgattgggtt cgcgactcgg aatgttcagc cgtaacgttg
     7741 gccttcgaac ctacaggcca ttgtgtcccc aaacgccaga cgtccatggc ctgcctcgat
     7801 gtctgtccaa gtttggtgaa tcctggcgcc aatgtctcgt agttcccgta gatgctcccg
     7861 cttcccaggg aatatcttgc cgttgctcgg gcggcatacc catcagtggt gtgatcttga
     7921 gcggcaatct caacataccg tctcggatca tggcctgaca cacctaattc agtgccgtca
     7981 tgctcagcga caatctcagg cgttagcact tcgaacacaa ctattctgta gggatccttc
     8041 cccgttcccg ccggtattcc cgcagttccg gcccagagcg tctcgccgcc ttcgtatttg
     8101 cggccacgac cgacgcctaa agctgtgatg gaataccctc gggtggcctc cactatatcg
     8161 caatttctcc cgtcccatac ccacaaaccg tttctgccgc ctatccacag cagcctgttc
     8221 caaacctcaa tggcagcagg atccttcact atcgaatcat ctacgacctt ggtccatcca
     8281 ccagcctcgt ccctagcata tacaccgtct ttggcggctg cgaaagtgcg ccaaccgtcc
     8341 accgcttccg cactggtcgc cgacacgatt gacgtcacgc ccaccccggc aagctcgtgt
     8401 tcaaaatgcg cgccctccaa cacggccacg ccgtcaccga accccaccag gatgagatcg
     8461 tgggccggat cataagcaat atcggaaatg gcgtccgaag gcaaccccga cccagcggac
     8521 ttcctgtaca ccgtccacgt gtcaagatcg gccaaatctg cacaggccac acccttgtca
     8581 gcagtgccaa cccaaacaga ccatggagtt gtagccacac aagtcaagtt gttgctgggc
     8641 aagcctgcag acatgtagat ccttcgccag ttactgacgt agtcgaatgg attctgcccc
     8701 gcaggcccgg gggttgcaga gaggaccgta agtcccccgt ctgttgcaaa aaaccagttt
     8761 tcgtcagcac tggccacgtc tcgaactaca ggggatgcaa gacccgagcc tatgctgtag
     8821 ttgcggaact catctccgcc aacgctcacc ccatcgctgt gggagatcaa caagtatgat
     8881 ttggactccg cgtcaactgc ccccatgatt ccggatattc tgttcgccgc atttgtcttc
     8941 cggttgtcgt caaacggaaa ctggtcatgg ctgaaagcca gatctgtgga gaggttgagc
     9001 cccttattgt cgtattgccc ggaaaagcct atgatcgcat gtgtattggg cattgtagag
     9061 gttgcttccg gcatagcggc cgattttgga tcatcagagt acacggcaac atccatcttc
     9121 aacttgagat tctcattcgg cgtccagcca aatatcgcac cataaaagtt ggacttgtaa
     9181 tcagctactg cgccaaacgg acccctgaag tactcgtact ccactttaac tacatcatcc
     9241 ggcccgagcg tcctcaatat ggtgagtatc ccgagctcgt agtcgatcgt gtagtcaatg
     9301 ttcttctgaa gaagcacatc gttgaggtat accttttcgc ttccttgagc aatggagagg
     9361 ccgagattga agatgcccat agccacagtg tggcggtact tgatacgcac cgcgtcataa
     9421 atatcgaaaa agcttggcgg aaaatcaaac tcgatcacac cgatatcgta agccacctgg
     9481 tagatcaggg gcaattctgt ctccacagac agaaacttgc cggccttgcg cacttccacc
     9541 gcaaccgatc caggaacaac gtcggtctgc cccagatcat agagacgcct gcgaagatag
     9601 gagtcggccc ttgcatctaa gacggttgtg ccaggtcccc catcctccat gcctgcaatg
     9661 atgtgggcgt ggcgcgaaat caggtcgtca aggaaagccg cctcggtctc actcccatag
     9721 acgaacggat atctcttctg agcgctgcct actagcccgt gctcggaatt gtaagaccgt
     9781 atcatcgact gaatctgacc tctcagaata tctcggatct ctgaccgcaa agctaacatc
     9841 tcttccgacg aagaaaccgt gatgaattgt ccgcgcgcaa gtggcacctt gccaccggca
     9901 gatatccctc cacgtgcttc cggatcctca agatacaagt aacccaagtc ccacaggttc
     9961 aaggtctctt ccagagtcct gccttccccg agttcgtctt cgtcatcata ccgaatccag
    10021 catcccatga aatccgggtc atatgagctt gacaacagat agtattccat gccgtcaatg
    10081 gaagccgaaa acccgctggc agacggcgac ggtccatacg tggccgcagg ctcgtagact
    10141 atcacttcct gagcagtatt gccctggaat gtcttctttg ccgcaattcc ctgcacccgc
    10201 ccgaacgtga cacccatttc cacctctgga aacttcgcgt tcaattcaag gccgcgcaaa
    10261 cgagcggaat acgaggtata tggggtctga ctcctaaaag acaggccgcc gaaacgaccc
    10321 tttaacgggt ctccgtcaag cttcagctcc atcacctgga ggttgccatc ttttcgattg
    10381 tccaggttgg cggagattgt cagccctggc gccacctgac cccgcacgtt gagtgagagc
    10441 aactgcatca ggtcgaagcg cccgggtgta taaccagttc caagaagagc agccgaatcg
    10501 ccccaagcgg acctcagttt gaactcaaca tcgcccccga tctgaagggg cgcttcctgt
    10561 ttctccgcag catatgcagg tgcttcgctc gccataaacg agatcaagac tgccgacgca
    10621 agcacaaaaa tgattagcga gaaagcgcgg cacgcactcg gcccgcgtct tctgcagcag
    10681 aaggctctca tctccgtcac ctctgacgcc acccgcaaat acagactgcc ggatcaactc
    10741 cgcagttcat ctgcggggaa ggcaatgtgg gctttctagc cccaaatccc taattcaaca
    10801 aaaacagggg gtcccctgca cggttgtgag aggacccccg cctagatcac ctagaacgac
    10861 atgcccaacg aaagccagtg tgaatcgggc agcgccgtat gaccctggta ggcgtagtcg
    10921 atttggaagt tctggactga gaagccggcc ccggccgtga atctcatgtc gccgtcttca
    10981 gcgaaaacgc ctcctgccct cactgcaaat tgaggcatga tcttatactc agcgcccaca
    11041 tgcccagttg cattgctgat gtcgaaatcc gcagctacca caagcttcgg aatgggcttc
    11101 atagaagcgc cgatgacata ttccggatcg aactggtcag tatagccgct ctgccatttg
    11161 gcggttccac ccacgtttcg cgcaaccgct cccagtctga acccttccat ccatggcatt
    11221 tcgatgaggg caccgacgtc tccggtgacg ccaaaccctt cattgttggg gattgtctgg
    11281 gagtatagct tgagtgatcc gccaaaagag aacatgccga agttccgagc atatccgccc
    11341 attaaggcgc cctcccggta gccgaattcg ttgccgggat tgccgtactc gtccttctcg
    11401 gtcaccgtcg acatcaggcc gatcacgccc actcccacgt tcttctgggc atatcccgcg
    11461 ccaatgtagt tgccggcacc atagagggtc gaatacaagg atgtgacctt gtgtccttcc
    11521 acattggcca gtcctgcggg attgtaatag atggcgttgg cgtcatcggc aaccgccacg
    11581 aacgctccac ccattcccag cgctctggcg cccatcccga tatcgaagac agccgccgtg
    11641 cccacaggat cctctgacgc cacggcgaaa gccggagtaa gcgcaacaac tgccatcgcc
    11701 gccagggcga ccattaccaa ccaactattc agtttcttcc tcatgatgtc accccccaat
    11761 tagcggctga tgacaagccg catagtatcg gacttgactc cgtctgccgt taccataaag
    11821 cagaggtaga gtccatttgc aagaggcttg tcagccttgt ccacgagagg ccacgcgtag
    11881 aaaaactctc cttctgcgat ttcctctgag aagacgagcc ttccagccac gtcatagacg
    11941 tagagcgtcg cgccgccaga agcattgacg tagaagttga ccacgtcgga cgctggcaca
    12001 gggcctgcca caaacgaatt gacccctgcg ttgatcacta cgaacttgcc gctgatacct
    12061 tcgcctgagt taccatagca atcagatgca atgaccttta tcaggcatgg accgctcttc
    12121 agcgccttgc tcacatccca ctcgtactct ccgttgttct ccaagccgct ggcaaacgtt
    12181 gtccacgtct cgccatcatc gaatgagtag gcaagcgtaa tggtgagctc cgcgtcctcc
    12241 ggatgcgttg cactccactt gacgtcgttc ttgccatacc actcgtcccc gttcacagga
    12301 gcaaccactt cgacctgagg ctcgataccg agccaatcaa ggacagtgcc catcagcatc
    12361 atcgggatcg tcagcggttc cggttcgggc tcctcattct ctgatacaaa cgccacagga
    12421 acctcatcgg gtattgcctc gaacatgaag gcaaagaagg tcaaccggtg ctccccttca
    12481 tatctgagtg cgcacggttt gccgttgtca ttgatgaaca caggcattgc accttcgcca
    12541 agctcgagcg agtcagtgcc atcgtcaaag ccctggctct cgaagtcgag ggcgatttca
    12601 aggccatcgg agataggatc tccggctact cccctcacct tctcagtgcc gacgtcatct
    12661 tccttgccca caacgtgcaa gtagttgcgg acaaactcgc tttcctcagg atcgatatcc
    12721 cacagcacct cagggcccat caggaagagc cgtccaccct tgtcgaggaa gtcggcaacc
    12781 gcagcctgtt cagcgtcgtc caaggaacca tactgtccac agttccatac gactctgaac
    12841 tgctcgaggc cggccttggc tccgccgttg ccgataacgt ccgaaggctc aactgtgaca
    12901 aactgcttct ccagtcttgc aaagagatct ttaaaatact cctcagtgtc aggagcactc
    12961 tcgtcatcgt cgacaaagac aatgccttcc tcatcgagga cgattacgaa gagctcggcg
    13021 cctacgactt caccttcggc atcagcaacc cacagcgttg gccagtacac gcccggctcc
    13081 gcataggcat gggttgcctt ggcctcttcg actcgggtct catcgccgaa atcccacaga
    13141 tacgcaattc caccgccctg gatgggatca ggatcatgcg cctctcccac aaaggcaatg
    13201 ttctgtccgg tcctggcaaa atagaggctt gcctctgcat tatcgatcac tggcggaatg
    13261 ttgatctcgc ccatcaagta ttcatatatc ttggtcaaca ggtatggcgt ggtattgatg
    13321 tctggatcat cgggatcgta gccatcccac gcatacatca gtgaagacca cggtgatgcc
    13381 agatagacaa gcctatatgt ctcgttggca tgacgcaggc ccacggcgcc cggaagatcc
    13441 atgattactc gttcctcatc accgtcttcc gtgagatccc acgccccgat cataattggg
    13501 ctggaaacga gatctggagc aattaggtca tccctaccgg tctgcacctg ccactccatc
    13561 tcgatgccgt cggttatcgg atcccccgag actcccttgt agatgtcgat tggcacattg
    13621 actccataag cgcccatgat atgtaagtag tcggcgccga accaatcgag gcccatgtcg
    13681 ccgagatagt cgggggctgc caggaacacc tttccgccat tatccatgaa gctcgccaag
    13741 aattcctggt cgaaaggaga aacgacttca aagcgggtga acccggtatc ccagaagata
    13801 gggttttcga cggcagtctc ggacaggccg agcggatacc tggtgtcaac cctcacgtat
    13861 gaaacccctg cagcctcagc cgcccacatg aagaaagcgt ttaccggtat cccaacatcg
    13921 aatgcaccgg tatcatccac gtagattatg gttcccggct tagccgccgt aacctgcacc
    13981 ggcatcttcg atatggatcc gtctaggcct ttggcataga accacgcaac gcccgagcct
    14041 tcatcttcaa gcgtcctgtt ggctacaagg ccgtacacgt tctcctggcc aaactgccac
    14101 caacattcct caacgccacc gggattctcg agcccagcct cgaatcttac agccaacggc
    14161 ggcgctccaa aggttacgtc cgcgcgtgcg tactcgatgc caggcggata tgtgcgggta
    14221 agcgcattgt aaatgttaag gcgcctgccc gaggttgtcc ttccaacaag atcaggcaga
    14281 gggtccccag acttcagaag aatgtccttg atcgtttcct gcccaggctt ccaaccaggc
    14341 gctccaggat aatgcggcag atggggatat tcggcgataa ctagcgccgc ggcgccggcc
    14401 acgtgcgggg tcgccatcga tgtgccgccc atccatgccc aggcgctctg attaccatct
    14461 ggatagcagg aaataatcat atggcccggg gctgcaatat ctaccgagtt cgcgccgtag
    14521 caagagaagt tggccagctt gtcattccat tcggtagcgg ccactgcgat cacgttcggc
    14581 agatcatagg aagatggata gtgcgggacg atatcgttgt caatgcctgc atttccagcc
    14641 gctgcaataa acaacatgcc ggagttggcg atcgcctctt ccagggccct gctgtaaccg
    14701 ccgccc
//
LOCUS       DAOSYL010000060        14541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3500_length_14541_cov_7.154908, whole genome shotgun sequence.
ACCESSION   DAOSYL010000060 DAOSYL010000000
VERSION     DAOSYL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_14541_cov_7.154908"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1542
                     /locus_tag="PLU15_08875"
     CDS             244..1542
                     /locus_tag="PLU15_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO31071.1"
                     /translation="MRSRLFAVISAVVLLLATCMGVNAAKQQLEYYSMWNPGEDQQQV
                     LAKVIADFEKANPGVSVKVTWAGRQVVAKARSRVIMGTPPDLIDQSFCELWGALLADK
                     EVAVPVNDLLDSRGPEGQKKFRDIFYAPTFDLYQVSGKSYFVPYEFITSGFFYDKTTF
                     KKYGLQEPKTWDDLMDVAGKLKSKGIAPFAQDGNISFYNAYWFYWLTNRILGPGSFYA
                     AAADRTGKTWDHPGYLKAAEMIYDISKARNGYFQKGYDGSNYPAAQMGWARRESAMIV
                     CGTWIPNETAEGRAADFDTGYFGFPEVKGGKGTLQNFEAYMIGFAIPKGAKNEELAKE
                     FIKFTLKKEYQEMIADKAQNIAARKDVKLPAVLADVEPYLRNATEFHMEYDGVQAKLP
                     EWFTSVFYGLDDKLLFGTITPNEFIRQIKQQTINYWQKKK"
     gene            1757..2656
                     /locus_tag="PLU15_08880"
     CDS             1757..2656
                     /locus_tag="PLU15_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31072.1"
                     /translation="MLKRNDKTFVIACLVPALVVYVVFFVFPALAALYVSLTDWSGFT
                     PNLEFIGLRNFRELFADEHFWKVVIRNTLGIIFIGGAIIFALAFLFSAILSSGIKSKR
                     LLRSIVFFPNIINPIGLAVMWSFIYEHDSGLLNNFLRAIGLGGLARTWAAPDRLFWSL
                     LVALVWIYVGFFVVILMAGFDRIPNDVIESAKIEGANDVRIFFSIKVPMIWDVLTMAI
                     VLWGIMALKEFAFLYSWGGGQAMPPEGAQNLAVYMYVMAFGTREPVYRMGYSTAMGVV
                     MLIMVILMVLIVRSLMRRESVEY"
     gene            2723..3637
                     /locus_tag="PLU15_08885"
     CDS             2723..3637
                     /locus_tag="PLU15_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31073.1"
                     /translation="MYRKKQPSRMSRGGLGVTPETGRVAAGDVGARIVRIIGWILIVG
                     WSIFSIFAFAWVVMTSLKSNHELFTNVWSLPKEPQWSNYVKVWTLSQLGTGFANSMLL
                     VLSSVAVLVVVASPAAYVLARKPFKGSDALTNFFTFGMGIPSQLLLVPLFFLMFKMKL
                     VDSLFGLAIVYVALSLPFTIFYLVGFFRTLPGELEEAAMIDGCTSFQAFSRIMLPLAS
                     PGVLTVSIFNFLFLWNDFLLALTFLSSNDKFTLSLGLFGLKGTMQYTGDWVGLFAGFT
                     LIMLPILIVYLFLSNQMVEGLTAGATKG"
     gene            3695..4753
                     /locus_tag="PLU15_08890"
     CDS             3695..4753
                     /locus_tag="PLU15_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO31074.1"
                     /translation="MVPRERVLAALRRQVPDRVPMYMEFTPPVVDKFHSITGSSDPAD
                     YFDLDIREVSFEPCKKVADFSMYLGELPPGSNVNEWGVGEVKGSGWSHFVDFVHPLQK
                     AATVKEIEDYPFPDMTQEYRWQHIPKAVKRWHELGYAVRGLPPFYGGTVFETAWALRG
                     LENLLVDFVQNRAMAGALLDRVTDISKGTATILAAAGVDVLVTGDDVGMQRTMMMSPS
                     MWREWLKPRLASVIEAAKEANPEVLIFYHSCGYIEPIIPDLIEIGVDILNPVQPESMD
                     PERIKKLYGNELAFWGTIGTQTTLPFGTPDEVRQEVKRMIETVGDGGGLLISPTHVIE
                     PDVPWENIMAFVDAVKEY"
     gene            5047..6318
                     /locus_tag="PLU15_08895"
     CDS             5047..6318
                     /locus_tag="PLU15_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO31075.1"
                     /translation="MSKSCRWSICLAVIFLIGLSTYVNAAASKITLNFIGMDQAGMTV
                     DEQKTVIREFEKLHPNIKVNATFVSYDALHDKLVTALAGGGRAFDVMLVDNTWLAELA
                     SAGWLLDVTDRVTPEMKRATWRIVTYNGRLYGMPWLMDQKYFFYNEEMLARAGYNQPP
                     KTWEELVEMSRVMKKKGIVEYPIIWSWAQRECANCDFVALLYGFGGRLENEKGQPAFN
                     EKGGLKALTFMVNSIREGISNPSSVVAVEEDVRHTLSSGKAAFAINWLYVYDLANDPA
                     ESQVAGKIKMSLVPVSEEVYKKGIVSATSNDSMGFAISKGSRNPDAAWELLKYFTSPE
                     VMNRYAAHQLPIWKDSFSDPALVKEQPVTMRMFQKQFPYGHVKVHVPYYMQYSKIMQL
                     MLQEALTGKKDPQQALDDAVRNIEIIQKKYK"
     gene            6507..7361
                     /locus_tag="PLU15_08900"
     CDS             6507..7361
                     /locus_tag="PLU15_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31076.1"
                     /translation="MLLILPSLVLIGGIVLYPILNNLVISFFDYNLTIPWARPFIGLR
                     NYVEVLTDREFLASVGRAAYFTVVSVSLELVLGLLIALFLNVDFRGRAFLKTMIILPW
                     AVPTVVNAIMWKWIYDANYGALNGLLMQLGMIDQYQAWLAKPFTAMNLVILADVWHQT
                     PFVVFILLATLSTIPESLYEAARVDGAGSLQAFRKITLPFLRPAFLIVLVIRTMEAFR
                     VFDIIYVMTAGGPANGTQVATYYTYVETFRYLNMGKGAAQSFIISALIMLVSIVYIRL
                     LKQDDTAY"
     gene            7373..8239
                     /locus_tag="PLU15_08905"
     CDS             7373..8239
                     /locus_tag="PLU15_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31077.1"
                     /translation="MKHKRRTILRSILIFIAGLLLLAVILLPYAWLVISSITETSELT
                     AVPPCWIPKRPTLQKYRDMFTPDVLGVSETAAMFKYAMANSAVVSFFVTVVTLIVASL
                     AAYAFARLEFRGREQMLLGIVAVKMLPAISTMVALYVLLSKVQLLDTRTGLVLVYLSF
                     TLPFAIWIMAGFFQTIPKELEEAARIDGTSRLGALFHVILPLSAPGLVSTAIFVFMMS
                     WDEFFYPLIFTSTYAAKTVPVAITEFAGRHAIDYSAMAAGGVLASIPPIVLAFIFQRY
                     IISGLTAGGIKG"
     gene            8246..9226
                     /locus_tag="PLU15_08910"
     CDS             8246..9226
                     /locus_tag="PLU15_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOO31078.1"
                     /translation="MPSRGVKEYDRRIAAVGNYNIDMVVGRVENLPTWGTEATVPYLQ
                     VRPAGSAGYTAIALATVGVGVTCIGNVGEDSYGVFIRDELAARGADVSGLLEADGADT
                     GQSVVIVRRDSERAFLSYNGHLAAFDEKCLMEAETAAVGFEQTEWVLLSGYFLLPGLG
                     YEATKRAFTRWKAMGKRVAFDPGWDTEGWPQHTVDEVTKLLQFVDLFLPNEDEARALT
                     QGSNPCSMASTLRDAGAGIVAVKLGADGAYVFDGEERIHMPAIKTEAFDATGAGDSFN
                     AGVLTGLTKRWTWNRTLKFANTLAGIVVSRKANRFPKLDEVYKKMKSQEE"
     gene            9236..10321
                     /locus_tag="PLU15_08915"
     CDS             9236..10321
                     /locus_tag="PLU15_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO31079.1"
                     /translation="MDYILKEILEESEAARTTIEKGDASQRQAAHAVLAADPDAIYAV
                     GCGSSYFAGMMARYPIEGFVHIPTIALSATDFITYSLDTITDASVVIGISQSGESFET
                     VEAIEKAKDRGALLVGLTNNPTSSIGHIADELILTYAGEEKASGTKTVLAQMLAAYQF
                     ALAMAELKSDIVSEAHRVLANELDKMPDTIGKLLERRDDAIRLAKELKDDSNLYLVGA
                     GPFYPLGLQIANTAREIARVHVNAFDVVEFRHGPLEIVESGTPVVFISNSYSPLRDNI
                     ATLASLVKRAGGRVIAATDESDVELNRAADIQVTMPSMSEIAGAIAYLSFFHMFLYNL
                     AVEKGLNPNEFRNIVKTWTQEPGEQEH"
     gene            10402..11130
                     /locus_tag="PLU15_08920"
     CDS             10402..11130
                     /locus_tag="PLU15_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO31080.1"
                     /translation="MSIDTESPMPLYYQIQRELTERIVRGDYSDSGKLPSESQLQAEF
                     NVGRATIRKAIDELAKRGLVVKHHGKGVFLAPPKMEQHLLSFYSFTDEMIDKGFTPST
                     EVLEFEYMTTLASVARALGLQKGTYVIRIVRVRSADDEPLIYETSYLPASLVPGLTRE
                     RVSDMPLYEIIEKDYGLRLSRAVESFEPVNLGPYEARILGVPAGIPALFLERTAYSDT
                     IPVEFCQGLVRGDRCRYQVELERP"
     gene            11287..12231
                     /locus_tag="PLU15_08925"
     CDS             11287..12231
                     /locus_tag="PLU15_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOO31081.1"
                     /translation="MIATFTLNAAIDRTLRVESLEKGIVNRATLTAARAGGKGINVAS
                     VAHSMGQKVIVSGFVGGHAGQFIEDYITSVGMVHDFVQIEAESRNCVAILESSTGTIT
                     EVNEAGPEVEYGALCAMEKKVEYLAERCGVCVFSGSLPRGCPQDYYARLIKIAKKQGA
                     FVVLDTSGSALCAAIEAKPDLIKPNEYEIQDLLGVEVSSEPQLIEALDEIRSRWSIDH
                     VIITLGPHGAICSIPTGFLRVLVPKLPVVNTVGCGDAFVAGICVGHERNLPAKEFIRL
                     GAAAASAKLGAKDAGTCDPEEVRRLVGEVQVEDIGQMG"
     gene            12324..13172
                     /locus_tag="PLU15_08930"
     CDS             12324..13172
                     /locus_tag="PLU15_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HOO31082.1"
                     /translation="MSLRSMTELLDTVEVGYAVIAFNFIGLDDLKGVMKAVERNQTPV
                     ILQVSQGTAAHLGLDYAVALARVAANVSAVPVCLHLDHATSIELILKAISSGFTSVMF
                     DGSRLPFEQNLEMTARVVEMAHDVGVSVEAELGNIGGKEDDPQGNGDGQVLVEPSQAC
                     EFVAATGIDALAPSVGTAHGLYKGTPDIRFDLISSLVELIDVPLVLHGGSDIPDAVLQ
                     KAIRLGMRKVNVGTDLQVAHVQALREALAETSGLPDMRKVHSRVIDAVCAMAESKIRL
                     GGLGRH"
     gene            complement(13333..13569)
                     /locus_tag="PLU15_08935"
                     /pseudo
     CDS             complement(13333..13569)
                     /locus_tag="PLU15_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(13894..14205)
                     /locus_tag="PLU15_08940"
     CDS             complement(13894..14205)
                     /locus_tag="PLU15_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31083.1"
                     /translation="MGWDDYHLHALNVGGVQVAPSEYADDMWDPPIEESDVTIADAAM
                     ASGGKFRYTYDFGDDWVHRITVEKNASPRRGRSIPDMYRRQTRLSARRHRRYLRLLFL
                     S"
     gene            complement(14220..>14541)
                     /locus_tag="PLU15_08945"
     CDS             complement(14220..>14541)
                     /locus_tag="PLU15_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31084.1"
                     /translation="GCCKTSLQQQGKSTCKKHLITVTGRSDDFDVNMQAMNMLYEAFP
                     ELLPHLLQTLCDTGPEASRKRSLVGIAKDTPDDPVYQLKIALERTSPPIWRRVMVPGH
                     FVFT"
BASE COUNT         3522 a         3220 c         4132 g         3667 t
ORIGIN      
        1 atcggcgcgc ggggttatca gccgtgtagt gaaaggggtc accatttacc cattttgggg
       61 gtaggtgcta catgttcggc agtgaggcgc gtgagagtcg tcgattcgaa ctacaggcaa
      121 ataagaaata cggggggtgg cgctcttact cgaattcgca cctgaagtta acaatttcat
      181 gtccctaagg gaagcgagaa cgccgtcata ggagcgactt agcaagcgag aggagtgaca
      241 tcaatgcggt cacggctatt tgcagtgata tccgctgtag tcctactgct ggctacttgc
      301 atgggagtca acgcggcgaa acagcaactc gagtattact ccatgtggaa tcctggcgag
      361 gaccaacagc aggtcttggc aaaggtgata gccgactttg agaaggcaaa tccaggtgtg
      421 agcgtaaagg tgacatgggc aggcagacag gtagtggcca aagccagaag cagggttata
      481 atgggaaccc ccccagatct cattgaccag tccttttgtg aactgtgggg cgcgcttttg
      541 gcagacaaag aagtagctgt tccagtcaac gatctacttg attcgcgggg acctgaaggg
      601 cagaagaagt tcagggacat cttctatgct cccacgttcg acttgtacca ggtgtcgggc
      661 aagtcatatt tcgtccccta tgagttcatt acatcaggtt tcttttatga caagacgacc
      721 ttcaagaaat atgggttgca ggaaccgaag acatgggacg atctcatgga tgtggctgga
      781 aagctgaaat cgaaaggcat tgctccattt gcgcaagatg ggaacatatc gttctacaac
      841 gcctactggt tctattggtt gaccaacagg atacttggtc ccgggtcctt ttatgctgcc
      901 gcagctgatc ggaccgggaa aacatgggat catccggggt atttgaaggc agcggagatg
      961 atctacgaca taagcaaagc ccgaaacggg tatttccaga aaggctatga tggaagcaat
     1021 taccctgctg cacagatggg ctgggctcgt cgagagtcgg cgatgatcgt ttgtggcaca
     1081 tggattccta acgagactgc cgaaggacga gcggccgact ttgacactgg atatttcgga
     1141 ttccccgaag tcaagggcgg caagggaaca ctccaaaact tcgaggcgta catgataggc
     1201 tttgcgatcc ccaagggcgc caagaacgag gaattggcca aggagttcat caagttcacg
     1261 ctcaagaagg aataccagga gatgatagcg gacaaggccc agaatattgc agctcgcaag
     1321 gacgtgaagc tgcctgcggt gctggcggat gtggaaccgt atcttagaaa tgctacagag
     1381 ttccacatgg agtatgacgg cgtacaggct aagcttccgg agtggttcac aagcgtgttc
     1441 tatgggttag atgacaagct cttgttcgga actatcacgc cgaatgaatt catacgacaa
     1501 atcaagcagc aaactatcaa ttattggcag aagaagaagt agtggttaag tcagttgata
     1561 ttttgtgtgc atgagaaccg gggcagacca tgggcgcgat cagtgcggag atggtttgcc
     1621 cctgtcctcc acgccgctga aaggatgcgc gataatccag ccccaacctg gttttaggcc
     1681 aatagactcg aaagtcgcct ttgcgatcga gttgggttga ggtctgatta tgggggctta
     1741 tctaagcgag gggtgcatgt tgaaacgaaa cgacaagacc tttgtaatcg cttgtctggt
     1801 gccggcgtta gttgtgtatg ttgtattctt cgtatttccc gcattggccg cgctatatgt
     1861 gagtttgaca gactggagtg gttttactcc caatctcgag tttatcgggc tcaggaattt
     1921 tcgcgagctc tttgcagatg agcatttctg gaaagtcgtt ataagaaaca ctctaggcat
     1981 aatcttcata ggcggagcca ttatcttcgc gctggcgttc ctatttagcg ccatactgag
     2041 cagcggaatc aaatctaaac ggctgctcag aagtatagta tttttcccga acatcattaa
     2101 ccccattggt cttgcggtca tgtggtcgtt catctacgag cacgactcgg gcctacttaa
     2161 caactttctc agagctatag gcttaggagg gcttgccaga acatgggctg ctccagatcg
     2221 gttgttctgg tctttgctgg tggccctggt atggatatac gtaggattct ttgtggtcat
     2281 tttgatggca ggtttcgacc gaattcctaa cgatgttatt gagtccgcca agatagaagg
     2341 agctaatgat gtgcggatat tcttttccat taaagtccct atgatatggg atgtgctcac
     2401 catggctatc gtgttgtggg gcatcatggc ccttaaggaa ttcgccttcc tgtattcatg
     2461 gggagggggg caggcaatgc cgccagaggg cgcacaaaac ctggccgttt acatgtatgt
     2521 gatggcattt ggaacccgag agcctgtgta ccgcatgggg tacagtaccg ccatgggtgt
     2581 ggtgatgctg attatggtca tcctcatggt gctcattgtg agaagtctca tgaggcgcga
     2641 gagcgtggaa tactgagaat gtggaaggtc gtgcgtgttc aaacatggcg cttgggttga
     2701 cattgtcccg aggaggattg acatgtaccg taaaaagcag ccgagtcgga tgagtagggg
     2761 cggtctgggt gtcacgccgg aaacgggaag agtcgcggct ggagatgtag gagcccggat
     2821 tgttaggatt ataggatgga tactgattgt tggatggtcg atcttttcta tattcgcgtt
     2881 tgcatgggtg gtcatgacct cgcttaagtc caatcatgag ctatttacca atgtgtggag
     2941 tctgccaaaa gagccacaat ggtcaaatta tgtaaaggtg tggacgctca gccaattggg
     3001 aacggggttt gccaacagca tgctgctggt tctatcatcc gttgcagttc tggtggtagt
     3061 ggcctcgccg gcagcctacg tgttagccag gaagccgttt aaggggagcg atgcattgac
     3121 caatttcttc acatttggca tgggtatacc cagtcaactt ctcttggttc ccctgttctt
     3181 cctgatgttc aagatgaagc tcgttgattc cctattcggg ttggcaatcg tctatgtcgc
     3241 tctttctctt ccgttcacaa tattctacct agtgggcttc ttcaggacgc ttcccgggga
     3301 gcttgaggaa gctgcaatga tagatggctg tacctccttt caggctttta gcaggataat
     3361 gcttccactt gcatcaccag gggttttgac tgtatcgata ttcaacttct tgttcctgtg
     3421 gaatgacttc ctgttggcgt tgactttctt gagttcgaat gacaagttta cactgtcgct
     3481 cgggctattc gggctgaagg ggacgatgca gtatacagga gattgggtgg ggctttttgc
     3541 aggattcact ctgataatgc ttccaatatt gattgtctat ctgtttcttt ctaaccaaat
     3601 ggttgaaggc ttgactgctg gcgccacaaa gggttgatga ccaagacatg cgccacgttg
     3661 cctttgtcca agatttctgg ggggatcaca cagcatggtt ccgagggaaa gagttctcgc
     3721 tgcactcaga agacaagtgc cagacagagt gccaatgtat atggagttta cgcctccggt
     3781 ggtggacaag tttcatagta ttaccggatc ttcggatcct gcagactact ttgatttaga
     3841 catacgagaa gtgagcttcg agccctgcaa gaaagttgcg gacttttcta tgtatttggg
     3901 ggagcttccg cctggcagca acgttaacga gtggggtgtt ggtgaagtca aggggtctgg
     3961 atggtctcac ttcgtagatt ttgtccatcc gcttcagaaa gcggcaacgg taaaggagat
     4021 cgaggactac ccattccccg acatgactca agagtacagg tggcagcaca ttcctaaggc
     4081 agtgaagcgt tggcacgagc taggatacgc tgtacgcgga ctgcctccgt tttatggggg
     4141 aacggtcttt gaaaccgcat gggcacttcg agggctagag aacctcttgg tcgactttgt
     4201 tcagaaccgg gcgatggcag gcgcactgtt ggatagagtt acggacatat caaaaggcac
     4261 tgccaccatt cttgcagcag ccggcgtcga tgtgcttgtg actggtgatg atgttggaat
     4321 gcagaggacg atgatgatga gcccatcgat gtggcgcgag tggctgaagc cacgcttggc
     4381 gtcggttata gaggcggcga aggaggcaaa tccggaggta cttatcttct accacagttg
     4441 cggctacata gaaccgataa tacctgatct catagagatc ggagtagaca ttctcaatcc
     4501 cgttcaaccg gagagcatgg acccggaaag aatcaagaag ctttacggca acgagttagc
     4561 tttttggggc actatcggaa cccagaccac cctgccgttt gggactcccg acgaagtgag
     4621 gcaggaagtg aagaggatga tagaaaccgt cggcgatggc ggaggacttc tcatctctcc
     4681 gactcacgta atcgaaccgg acgtcccatg ggagaatatc atggcctttg tggatgctgt
     4741 gaaagaatac taaggggcta gacccgtaaa ggcgcctttc gcgccccaac actcgtcggc
     4801 tattagtcca gcaaagcgat gctgcgatgc cctgcagaca ctgactgcag ggcatcgctg
     4861 atattccctt ttgtttttga agcaggaatt cgtggcagcg cgtagaaatg tttctaacat
     4921 atacgcatat gcgtacaagt tgagggggga cgcgataggc aatccgtgtg ttaaggcagt
     4981 ggagtttgac aggacagcag gtcatagcag acggtatcac taatttccgt gaggagggtc
     5041 cactctatga gcaagagctg tagatggtca atttgtctag ctgtcatttt tcttattggt
     5101 ttgtcaacat atgtaaatgc tgctgcatcg aagatcacac tcaacttcat tggtatggat
     5161 caagctggaa tgaccgtgga tgagcaaaaa accgttattc gcgagttcga aaagttgcac
     5221 ccaaacatca aggtgaacgc tacttttgta tcgtatgatg ctcttcatga caaactcgtc
     5281 acagcgcttg ccggaggtgg acgtgcattc gatgtaatgc ttgttgacaa tacctggctt
     5341 gcagaactcg catctgctgg ttggttattg gatgttactg atagagttac ccccgagatg
     5401 aagagggcta catggcgcat tgttacatat aacggcaggc tttacggtat gccatggctc
     5461 atggaccaga agtatttttt ctataacgaa gagatgctgg cacgggcagg gtacaatcag
     5521 ccgccgaaga cgtgggaaga actagttgag atgtctcggg tcatgaagaa gaagggtatt
     5581 gttgagtatc ccatcatttg gagctgggcg cagcgcgagt gtgcgaactg cgactttgtg
     5641 gcactccttt acgggtttgg cggaaggctt gagaatgaga agggccaacc tgcgttcaat
     5701 gaaaaaggcg gcttgaaggc cctaactttc atggtgaaca gtatccgtga gggtataagc
     5761 aacccatctt ctgtagttgc ggtggaagag gatgttcgac atactctatc gtctggaaag
     5821 gcagcctttg ccatcaactg gctttacgtc tacgacctgg ccaatgatcc ggccgagtct
     5881 caggttgcag gaaaaatcaa gatgagtctt gtgccggtct cagaagaggt ttacaaaaag
     5941 ggcattgtca gtgccacgtc aaacgactct atgggatttg caatctcaaa gggctcacgc
     6001 aatccggatg cggcctggga gcttcttaag tacttcacca gcccagaggt tatgaacagg
     6061 tatgcggccc accagctgcc catatggaaa gactcgtttt cagatccagc gctcgtgaaa
     6121 gagcaacccg tcacaatgag aatgtttcag aagcaatttc catacggtca tgtcaaggtg
     6181 cacgtaccat actacatgca atactccaag ataatgcagt tgatgttaca agaagccctc
     6241 acaggcaaga aggatcctca gcaggctcta gatgatgcag taagaaacat agagataatc
     6301 cagaaaaagt ataagtagta gtctcatggt atgaccgtat ggataatgat tgagaaatta
     6361 tccgacctgg ggatacttct ccccaggtcg ggagcgacaa ggggagaaca ggaatgatgt
     6421 cggggccttc cggcttgcct aatacagggc cgttagggag catcgggctt ggcgcaaaga
     6481 ggggtctgtc tgaaagcagg cttgccttgc tgctgatctt acccagtctg gtgctcatag
     6541 gcggaattgt gctttatcct atccttaaca acctagtaat cagctttttt gactacaatc
     6601 tgactatacc gtgggcgcgt cccttcattg gtctgcgaaa ctatgtcgaa gtattgaccg
     6661 atagagaatt cctagcatca gtaggacgag cagcttattt cacagtagta tctgttagcc
     6721 ttgaactagt gctcggctta ctaatagctc ttttcctgaa cgttgacttt cgagggcgtg
     6781 catttctgaa gaccatgatc atattgccgt gggcagttcc cactgtggtc aatgcaatca
     6841 tgtggaaatg gatctacgat gcgaattacg gggccctcaa tgggctactg atgcagcttg
     6901 gaatgataga ccaataccaa gcctggctgg ctaagccgtt caccgcgatg aacctcgtta
     6961 ttttggccga tgtgtggcat cagacaccat ttgtggtatt catactgctc gccacgttat
     7021 cgacgattcc agagtctttg tatgaagcgg caagagtcga tggagccggg agccttcaag
     7081 catttcgaaa gattacgctc ccattcttgc gtccagcatt tctcattgtt cttgtgatca
     7141 ggactatgga agcatttcgg gtgtttgaca taatttacgt aatgacagct gggggaccag
     7201 ccaacggtac tcaggttgct acgtattaca catatgtaga gacattccgt tatctgaaca
     7261 tgggcaaggg agctgctcaa tcgtttatta tctcagcact cattatgctt gtgtcgattg
     7321 tatatatcag acttctgaaa caggatgaca cagcttacta ggaggatagc cgatgaagca
     7381 caaacggaga acgatattga ggtcgatctt gattttcatt gccggcctgc tccttctggc
     7441 agtaatactg ctaccgtatg cttggcttgt gatttcgagc attacggaga catctgagct
     7501 tacagctgtt ccgccctgtt ggattccgaa gcggcccaca ttgcagaagt acagagacat
     7561 gtttacgcca gacgtgttgg gagtctctga gacggcggca atgttcaagt atgcaatggc
     7621 caatagtgcc gtggtctcat tcttcgtgac cgtggttacg ctcattgtcg catcgctggc
     7681 ggcgtatgct tttgcgcggc tggaatttcg gggaagagag caaatgctcc ttggaatagt
     7741 ggcggtgaag atgctgcctg ccatttctac tatggtagcc ttgtatgtgc tgctcagcaa
     7801 ggtgcagctg ctcgacacta gaaccggttt ggtcctggtc tacctgagct tcacgttgcc
     7861 ttttgccata tggatcatgg ctggtttttt ccagacgatt cccaaggaac tggaggaagc
     7921 ggcacgtatc gacggcacct cgcgtttggg tgcccttttc cacgttatcc ttccgctctc
     7981 agcaccaggc ttggtttcta cggcaatatt cgtgttcatg atgtcgtggg acgagttctt
     8041 ctatccgctc atattcacca gcacgtacgc agcgaagact gtaccagttg cgatcactga
     8101 atttgcgggt aggcacgcca tcgactattc tgccatggct gcaggaggcg tgcttgcaag
     8161 tattcctccc attgtattag cgtttatatt ccagcgttac atcatttctg gccttacggc
     8221 tgggggaatc aaggggtaga tgatattgcc aagtcgtggc gtgaaagagt acgaccgccg
     8281 gattgcagca gttggcaact ataacataga tatggttgtg ggacgtgttg aaaaccttcc
     8341 gacgtgggga actgaggcca cagtgccata tttgcaggtt aggcctgcag gttcagcggg
     8401 atatacagcg attgccctag ctactgtagg tgttggcgta acgtgcattg ggaacgtggg
     8461 agaggattcc tatggtgttt tcatccgtga tgagctcgca gcgcgtggag ccgacgtgag
     8521 tgggctgctg gaggccgatg gggctgacac aggccaaagc gttgtgatag tccggcgtga
     8581 ctcggagagg gcttttctta gctacaacgg ccaccttgct gcgtttgatg aaaagtgcct
     8641 aatggaagcc gaaacggctg cggtcggttt tgagcagaca gaatgggtgt tgctatcagg
     8701 atattttctc cttcctggtt tgggatatga agcgacgaaa cgagctttta cacgatggaa
     8761 ggcgatgggc aaaagagtgg cattcgatcc gggctgggac actgagggat ggccacagca
     8821 tacggttgac gaagtgacta agcttcttca gtttgtggac ctctttctgc ccaatgagga
     8881 cgaggcgcga gcgcttacac agggatccaa tccgtgcagc atggcgtcga ctcttcgtga
     8941 tgctggtgct ggaattgtgg ctgtgaaact gggcgccgat ggcgcatatg tgttcgatgg
     9001 agaagagcgc attcacatgc ctgcaatcaa gactgaagcg tttgacgcta ctggcgcagg
     9061 agactcattc aatgctggtg tgcttacagg tttgaccaag agatggacat ggaacagaac
     9121 actcaaattt gccaatacac tggctgggat agttgtgagt cgcaaggcta accgatttcc
     9181 taaactagat gaagtatata agaagatgaa gtcacaagag gagtgaacgt tctacatgga
     9241 ctacatactt aaggaaattc ttgaggagtc agaggctgca cgcacaacta tcgaaaaggg
     9301 ggatgccagt cagaggcagg cggcacatgc ggtactggct gcagatcctg atgcgatcta
     9361 cgcggttgga tgtgggtcat cctattttgc tgggatgatg gcgagatacc ctattgaagg
     9421 gttcgttcac attcccacta ttgcgttgtc ggctacggat ttcatcacat actcattgga
     9481 caccattacc gatgcgtcgg ttgtgatagg catatcccag tcaggggaga gttttgagac
     9541 agtggaggcc atagaaaagg cgaaggatag gggagctctg cttgttggcc ttacgaataa
     9601 tcctacaagt tcaattggtc acattgctga tgagctgata ctaacatatg caggcgagga
     9661 aaaggcatct ggcacgaaaa cggtgcttgc tcagatgttg gctgcctacc agtttgcatt
     9721 ggctatggcc gagctcaaga gtgatattgt ttcagaagcc caccgcgtac ttgccaacga
     9781 gttggacaaa atgccagata caataggcaa actgctggaa agacgggatg acgctatcag
     9841 acttgcgaaa gagctgaagg atgactccaa cttgtacctg gtaggagctg gcccattcta
     9901 tcctctggga ttgcagatcg caaatactgc tagagaaata gccagagttc atgtaaacgc
     9961 ctttgatgtt gtagagttca gacatgggcc gcttgagatc gtggagtcag gcacacctgt
    10021 cgtattcata agtaactcgt attctccgtt gcgtgataat atagccacat tggcatcgtt
    10081 agtaaagcgg gctggtggta gagtaattgc tgcaactgac gaatctgatg tggaactcaa
    10141 ccgtgcagcg gatattcagg ttaccatgcc ttccatgagt gagatagctg gcgcaattgc
    10201 gtatctgtca tttttccaca tgttcttata caacctggcg gttgagaaag ggttgaatcc
    10261 gaacgaattt cgcaatattg ttaagacctg gactcaggaa cccggtgagc aagaacacta
    10321 gatagatatg gcatgttgca tgcggacatg cgctgtgccc actttgacgg gcatgcgctc
    10381 gaccaaaggg ggaatgtagc cgtgtctata gacacagaaa gccctatgcc actttactat
    10441 cagatacaac gggagttgac tgagagaata gtcagaggtg attacagtga tagcgggaaa
    10501 ctgccctctg aaagccaatt acaggcggag tttaatgtag gaagagccac tattcgcaag
    10561 gccattgacg aattggctaa aaggggactg gtggtgaagc accatgggaa aggggttttt
    10621 ttggctcccc ccaagatgga acagcatcta ttgtcattct acagcttcac ggatgaaatg
    10681 atagacaagg gattcactcc aagcaccgaa gtgttggaat ttgaatatat gactacgctt
    10741 gcaagcgtgg caagagcatt ggggcttcag aagggaactt atgtcatacg cattgtcaga
    10801 gttcgttcgg cagatgatga gccgttgata tacgagacct catacttgcc agcttcattg
    10861 gtaccgggtc tgacgagaga acgagttagc gacatgccat tgtacgagat aatcgaaaag
    10921 gactatggac tgaggctatc tcgcgcagtt gagtcttttg agcctgtgaa tctgggaccg
    10981 tacgaagcgc gtatcttggg cgttcctgcg gggatacctg ccttgtttct ggagagaacg
    11041 gcttattcgg atacgatccc agtggaattc tgtcagggtc ttgtgagggg cgaccgctgt
    11101 cggtaccaag tggaattgga acgaccgtga tggagcttca gctctgacgc tatgggaatg
    11161 ctgtgtgagt atatgctact gcgaactatg cgatgtcctg tggagcatgt gtctttcgca
    11221 gccgtctagt tgagcgtctg actgctcgat cgccatgcga gttcgctttg aaggaggatt
    11281 gtatgagtga ttgcgacttt caccttgaac gcggctatcg acagaacctt gagggtggaa
    11341 tcccttgaaa aaggcattgt gaacagggca accctcacag cggccagggc aggaggcaag
    11401 gggataaatg tagccagtgt cgctcattca atgggccaaa aagtgatagt ctcgggcttt
    11461 gtgggcgggc atgcaggaca attcatagaa gattacatta cttcggttgg aatggtccat
    11521 gatttcgtgc agatcgaggc cgaatccagg aattgcgtag ccatactcga gtcgagcaca
    11581 gggacgatta cagaggtgaa cgaggctggt ccggaggtgg aatatggtgc cctctgcgcc
    11641 atggaaaaga aggttgagta tttggctgaa aggtgcggag tgtgtgtctt ttccgggagc
    11701 cttccgcggg gctgtcctca agattattat gcccgattga tcaagatagc caagaaacaa
    11761 ggggcgtttg tagtgctcga tactagtggg tcggctcttt gtgccgcaat cgaggccaaa
    11821 cccgatctga tcaagcccaa tgaatacgaa atccaagacc ttttgggggt tgaggtgagc
    11881 tcagagcccc agttgattga ggcgttagat gagatcagaa gcagatggtc gattgatcat
    11941 gtaattatta cattggggcc acatggcgcc atttgctcca ttcctaccgg atttctgaga
    12001 gtgctagtgc cgaagctacc cgtggtcaat actgttggct gtggcgatgc attcgttgcc
    12061 ggaatatgtg ttgggcatga acgcaatctg cctgcgaaag agttcatacg attgggagcc
    12121 gcggctgcat cagccaagct cggagcgaag gatgcaggca catgcgatcc agaggaagtc
    12181 cgacggttgg tcggcgaggt gcaagtggaa gacattggtc agatggggtg aggagactgc
    12241 gaagctcaag gggcacctcg tgacgttggc gccaatcatt ctgtctattt gctaccggtg
    12301 gctgaacaat agggggtggc gatatgagtc tcagaagcat gacggagttg cttgatactg
    12361 tagaggttgg gtatgcggta attgctttca actttatagg tctggatgat ttgaagggcg
    12421 tgatgaaagc cgtagagagg aatcagacgc cagtcattct tcaagtaagc caaggaactg
    12481 cggcccatct cggcctggat tatgctgttg ctcttgcccg ggttgcagcc aatgtttccg
    12541 cagtgccggt ctgcctccat cttgaccatg cgacaagcat agaactgatc ctaaaggcca
    12601 tatcgagcgg gtttacctca gtgatgttcg acggatccag gctgcccttt gagcagaacc
    12661 tcgagatgac tgcgcgagtg gttgagatgg cacatgacgt gggagtttct gtcgaggcgg
    12721 agcttggaaa cataggcggc aaagaagacg acccgcaagg aaatggagat ggtcaagttc
    12781 ttgtcgagcc atcccaggcc tgcgagttcg tggcagctac aggcatagat gccctagcgc
    12841 cgtcggttgg cactgctcac gggctttaca aaggtacgcc agacatcagg tttgatctga
    12901 tatcctcact tgttgagctc attgatgttc cactggtgtt gcatggaggg tcggatatcc
    12961 cggacgcagt cttgcagaaa gccattaggc tcggaatgag aaaagtgaac gtgggaactg
    13021 atcttcaggt agctcacgtt caagccctga gagaagcctt ggctgagaca tctggccttc
    13081 cggatatgcg aaaggttcat tcacgcgtca tcgatgccgt atgtgccatg gctgaaagca
    13141 agatacgttt ggggggactt gggaggcatt agcctccgga tgtcattggc ataaggtttt
    13201 ctcatcgtct ggtgtgctcc gggagtaaaa tcaatttggc aatccaaaag ttcatagttc
    13261 ggcagtcaaa agtgcatagg ttaaccacta cgcccggggt cgaaattcga ggccctacgg
    13321 gattagtccg ttctagcaaa tagtgccttg ttggaggtgg gcgcccccga cgtcatctgc
    13381 ggcatgcaat tgggagaggg catgccgtgg acggtagctc tcgcctgtga atgcaagcac
    13441 atgtctatga tgaacaaacc catcgatgag cacgccggta agccgattgt cgttaatcac
    13501 cgtgttccat tgtccgaact caaggttcgt ggtgatggcc acgctttggc gctcatagca
    13561 gctggcgatt gattggccca gaagcccatc tcatccacga acagggtttt gccaacactg
    13621 taagcgtctc tcgacagtga tttgagaatt gagttggagt catggcgata atggacaata
    13681 caggaaagat agaggaggac atgagcatca tgggaataag gaaaaagctc tgctcctcgg
    13741 cctatccggt aaagtgcagc gaccgtggca gcgctgaata ggatcattca ggcctgacta
    13801 gtctcacttc atctgttttc tcataaactc aagactgcta ttgacaactc gacggcgtcc
    13861 ccatgcatct ccgggttggg gtctgccaag gcttcaagat aggaataata gccgtagata
    13921 ccgccgatgt cttcgggcgg acaggcgcgt ctgccgcctg tacatatcgg gtatcgaacg
    13981 tcctcttcgc ggggaagcgt ttttctcaac agtaattctg tgaacccagt cgtctccaaa
    14041 atcgtaagta taccggaact tgcctccaga ggccatggcg gcatcggcta ttgtcacgtc
    14101 agactcctcg atcggaggat cccacatgtc gtctgcgtat tcgcttggag ccacttggac
    14161 tccaccgaca ttgagcgcgt gcagatgata gtcgtcccaa cccattaccg cctggattat
    14221 taggtgaaga cgaagtggcc gggtaccatt accctgcgcc atatgggcgg actggtgcgt
    14281 tcaagggcga tcttcagctg atacacgggg tcatcgggtg tgtccttcgc gatcccgact
    14341 agtgatctct tgcgcgaagc ctcgggaccc gtgtcacaca atgtctgcaa caggtggggc
    14401 aatagctccg ggaaggcctc gtacagcatg ttcattgcct gcatgttgac gtcgaagtca
    14461 tcgcttctgc ctgtgactgt gataaggtgt tttttgcaag tactttttcc ctgttgttgc
    14521 agactcgttt tgcagcatcc c
//
LOCUS       DAOSYL010000061        14276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3579_length_14276_cov_8.478940, whole genome shotgun sequence.
ACCESSION   DAOSYL010000061 DAOSYL010000000
VERSION     DAOSYL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14276
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3579_length_14276_cov_8.478940"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..2170
                     /locus_tag="PLU15_08950"
     CDS             308..2170
                     /locus_tag="PLU15_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO31085.1"
                     /translation="MKLKIGGKIVLSYTVVLLVMAAISILGIQADRKITNEYMNLIER
                     SLPGETIIYRLRSELLEKGLALRGYILSGNPAEIERFHLLDENVEQSMDKARMLSQAG
                     KSLQSLELLAEINDRYDLLAEDISHLVQLGMTEEATALMADEGALILGQVDDVIAEWV
                     EFIEGLNDNWVNTAKATGQSKDLFSLVGMIANIAIAVIMTIFLNRNITKPVKTIQVAA
                     DAVAQGDLTVSVPILNTGDEIADLATSTRKMVENLTNLLSEMQNEAEQVASTSEELSA
                     SGEESAHAAQQISAAIQEMASGAEQQSANAGETAAGAQQVAAAIEQIADGAGQQTQQL
                     QNVSRLIAQMGQELDHVNDFVMKMEEAINATAKEAEKGDRSVAQVAASMERIKNSSNE
                     VEQAAADLEASSRQMVQVVQVIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVR
                     KLAEGSLNETKVISGLIEQTLAEVGKVASAIRGTNDLVEESLPLVIESTEVLHNISNH
                     AANNLELAHSVVESSNSLMEEASDVNEATVEVVAVADENAAASQQMAASIQQVQKAIE
                     NVAAISEENAASVEKVAASTEQVTASLQQISSASHSLADMAARLQQLAARFKLA"
     gene            2372..4234
                     /locus_tag="PLU15_08955"
     CDS             2372..4234
                     /locus_tag="PLU15_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO31086.1"
                     /translation="MKFKIRGKIIFSYIIVLLVMLIMSVANIHADRVTTKHYANLVEQ
                     NLPLETIVWKVRSEILEKGIALRGYVMTGNPATIVKFYTIDDDVLQLFDEMQPLCEDK
                     ESQRALELLTDINNRYNSFADDISVFVEFEMMDEAYALIEEREVFLIDELDQTIHNWI
                     QLIQDQNRQSIAKAESASRINDLISLVGMIANIAIAVVMTILLNRNITKPVKTIQVAA
                     DAVAQGDLTVSVPILNTGDEIADLATSTRKMVENLTNLLSEMQNEAEQVASTSEELSA
                     SGEESAHAAQQISAAIQEMASGAEQQSANAGETAAGAQQVAAAIEQIADGAGQQTQQL
                     QNVSRLIAQMGQELDHVNDFVMKMEEAINATAKEAEKGDRSVAQVAASMERIKNSSNE
                     VEQAAADLEASSRQMVQVVQVIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVR
                     KLAEGSLNETKVISGLIEQTLAEVGKVASAIRGTNDLVEESLPLVIESTEVLHNISNH
                     AANNLELAHSVVESSNSLMEEASDVNEATVEVVAVADENAAASQQMAASIQQVQKAIE
                     NVAAISEENAASVEEVAASTEQITASLQQMSSASHSLADMAARLQQLAARFKLA"
     gene            complement(4260..6086)
                     /locus_tag="PLU15_08960"
     CDS             complement(4260..6086)
                     /locus_tag="PLU15_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO31087.1"
                     /translation="MKAEINLPSTAVVLSLDPRLEASIRVAVSLRREPFPSDADMRII
                     TCSTRAGAAEALARYVGQPVLSIVLVDVSPGGGFTLDDVTLMARQFPTIPVVAVVPRN
                     KEDLEYEALRAGACDYLIKGVITPSIFARMTRSAENYWSAKSLAFEGLEKAKTLAQAI
                     PIALMLVDDRGIVIGGWNPAAEEVFGVARRDALGRPMPAVKWTGKEETAAILARAVAG
                     DSISGIEVQCRRADGELIDAIAFFTALYEGDELKGILALFEDITQRKRAENALRDSEV
                     RFRGVFEGSQLGMAIADTNMRIIHANPALGELFGCLPEDLAGVMAEELIHPDDEADIA
                     SRVSRLLRDRISFFTAESRYVRMDGTVFVGRLLATLFSVSLEGPHYVLFALEDITLRK
                     IAQDQLEASLVKLAQTTEGAIHAMSRVVEMRDPYTAGHQERVACLARHIAMELGYPKD
                     AVDGIYFGSLVHDIGKIVVPGEILTKPGKLSPIEFSLIKAHPEEGFKVVNSIEFPWPI
                     ANMVLQHHERLDGSGYPAGLAGDAICEGAKIIAVADVVEAMSSHRPYRPSLGMDAALD
                     EIVSKSGILYDPEVVQACVNVIAVHGDRALMGTVSDLNGEMP"
     gene            6392..6949
                     /locus_tag="PLU15_08965"
     CDS             6392..6949
                     /locus_tag="PLU15_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31088.1"
                     /translation="MDNGTYEEIGKKSLAAFSNLLDILSGARTSPALADPVGLERRIG
                     EQLATVVASLDLVRRDLAALASRTDRALLELLKRIEPQEGLAVTSFPTPSLPASQVSA
                     IGTAGPGTGGRAATPSKPRGKYERVPKEQTREKILEAARELAAAGRKITLAECARMAD
                     VAYYKAVYACKDSEELEELMSGNAG"
     gene            complement(7856..8722)
                     /locus_tag="PLU15_08970"
     CDS             complement(7856..8722)
                     /locus_tag="PLU15_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOO31089.1"
                     /translation="MGKDRLGESCVREAYEPRKYRELFSAQDVIYFRCAVGQTDLQVG
                     VGFSCPDFRGKLPEESFLRDHVSGVVLRARRDIENYIATHPEFLTSLIPVIPRPWAPP
                     IVRWMCACAAQAGVGPMAAVAGAISETVGISLLSEDSLSKLWQREPDIGNPDGHRARI
                     SEVIVENGGDVFIASSRLRRVGIYAGTSPLSGKIALEIAPGQTPCGICTSSGTVGHSR
                     SFGRCDGAVIVAKDTALADAVATRVANVVKSREDIEEALDTASKIPGIVGAVIVIGDA
                     LGAWGDIKLAPA"
     gene            complement(8733..9191)
                     /locus_tag="PLU15_08975"
     CDS             complement(8733..9191)
                     /locus_tag="PLU15_08975"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO31090.1"
                     /translation="MTVERTVGADGITDATTAKRVKALLTYPPGAAGKPITYSLIREF
                     DLWINILHAEIAADKAGKLIMDIQGSVEDVDRGLEFLDEEGIGYEILNGSIVWDEDKC
                     VHCGACTAVCPSHALTMDNCDWSLVFDKEKCYLCRLCARTCPVGAMSSPI"
     gene            complement(9194..10357)
                     /locus_tag="PLU15_08980"
     CDS             complement(9194..10357)
                     /locus_tag="PLU15_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOO31091.1"
                     /translation="MGYRSFEEINEKIRSKKAVVVTAEEIIEIVDEKGVEGAFRDVDV
                     VTCGTFSPMCSSGAFLNFGHSDPPIRMSRVWLNDVPACAGLAAVDAYIGATELSETAG
                     MEYGGAHVIEDLIAGRKVRLKATSYGTDCYPRRALEAWITKDSINEAYMFNPRNCYQN
                     YGAATNSSDRTIYTYMGTLLPHFGNVNYCTAGELSPLINDPFYRTIGLGSRIFLGGAQ
                     GYVAWQGTQNNPSQPRAENGVPIGGAACLALIGDLKQMDPRYIRAARFEKYGVTMFVG
                     VGTAIPLLDEEMVRFVAVQNRDIKTKVFDYSVGRRSKPALAEVSYEELRSGSVQIGSR
                     TIPTAPLSSLKVAREIAQTLKGWIARGEFLLQQPVAALPLEGSPTTLDNCEGE"
     gene            complement(10358..12823)
                     /locus_tag="PLU15_08985"
     CDS             complement(10358..12823)
                     /locus_tag="PLU15_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOO31092.1"
                     /translation="MSVRVEDLIRGDEVFVFDGAFGTMLQARGLPPGACPEVWCLKCP
                     EEVERVHRQYIDAGAQVIETCTFGGTALRLGHFGLAHAAREINLAAVEIARRAAADRG
                     TLVAASMGPLGALVEPLGEVSFDEAYLQFAEQAEAFALARPDLIIIETIADLNEMRAA
                     LLACRDRAPDIPVIAQMTFDSTGRTFTGTPPESAALVMESMGASVVGVNCSVGPDLLA
                     DVVARMGAVTSKPISVQPNAGLPHVGPSGRGEYPMGPEEFASYGPKLVSAGASIVGGC
                     CGTTPEHIARLRSALEGLRPIVRRREPELGITSRTQPLFFADAELPAIVGERINPTGR
                     RKLAQDIVDGSFELVRKEAVAQAKAGAQALDVNVGTPLANEPWAMYEAVKAIEATTRL
                     PLCLDSSSAEALEAGLRAYAGRALVNSFSLERGREEDVLRLAARYGAAVIGLPIDENG
                     IPATAEDRLRLAERLVSSAQSHGIPQNAVIIDALAMPCGAKQEQAVETLKAIEMIRKE
                     LGCKTSLGISNVSFGLPSKPVLNTAFLMLAIAAGLDMAIANPLDEAMMAQVRAARVLL
                     GRDVNAERYILWAGAASPAPHGVAADPGGKKKLTLIYDAVLGGDKDLAVRFVEEAVSE
                     GEDPSSILNDALIPAIEETGRRFASGAYFLPELMLSAGAMQAATEVIRSRVGSAEDAP
                     ASRGTVVIATVEGDIHDIGKNIVSMFLESNGYNVVDLGKNVKARDVLAGAKKAGADVV
                     CLSALMTTTAPKMRESIELFSSEGYDCPIIIGGAATSREYAIRIGADGYGSDAVEAVF
                     EVERLLARKRAFGRQSDQVGGKE"
     gene            complement(12820..13755)
                     /locus_tag="PLU15_08990"
     CDS             complement(12820..13755)
                     /locus_tag="PLU15_08990"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOO31093.1"
                     /translation="MARLLNRLRQGSFAVTVEVNPPKGADVSKTLALVRRFSDRVDAV
                     NVADCPMANVRMSPITLAHLIQRDVGVEAIFHLTCRDRNTIGLQAELLGASGLGVRNI
                     LALRGDPPSRGDHPEAHGVFEVDAVGLISLAAALNSGHTVGGGELGEGTDFAIGCVAN
                     PNAEDLEAEISRLEEKIAAGAHFVQTQPIFDVRAYERWANAIDGRVSIPILYGILPIK
                     DAAFAVHLKENVPGMEVPDWVIDRMRSGGDGEGYRLAAELLSEIGKDIAGIHIFPMNS
                     SKRVVGVLDALAEEGFAVPPVQTACDGLRKVAPNG"
BASE COUNT         3393 a         4321 c         3682 g         2880 t
ORIGIN      
        1 ccctcatcaa ttcctcttgt tcagctagat tgtttctgac attcactaca atccacggca
       61 aaaggacaat tatgagaaca agcgcgaaac ggttgcgcag acttgaaaca ttaatcttcc
      121 ccacgtttgg tacctcccca ccttaccgca ggtttggtag atgagcgatg ccaatgcccc
      181 attatctata tcggcataaa ccgtgtcagt ctatatcaaa ttatatcgaa aattttgatg
      241 tcgtacctaa tatcggtgct tgcgtttgcc gataagatta ttgcatgacg caacaggagg
      301 taggccaatg aagctcaaga taggtgggaa gatcgtactg tcgtacacag tcgtcctttt
      361 agtcatggct gccataagca tactcggcat tcaagctgat cgtaagataa cgaacgaata
      421 tatgaacctg atcgagcgga gcttgcccgg agagaccata atatacaggc ttcgcagcga
      481 gcttttggaa aaagggctgg ccctgcgcgg ttacatactc tcgggcaatc ccgcagaaat
      541 cgaaagattc catctgctcg atgagaacgt tgagcagagc atggataagg cccgcatgct
      601 gagccaggct ggcaaatcat tgcagtcgtt ggagctattg gccgaaatta acgaccgcta
      661 tgatttgctc gccgaagaca tctcacacct tgttcagctt gggatgacag aagaggcaac
      721 cgcattgatg gctgatgaag gcgctttgat cctcggacaa gttgacgatg tcatcgctga
      781 gtgggtcgag ttcattgagg gcctgaatga caattgggta aacacagcca aggcaaccgg
      841 ccaatcaaag gatctatttt ccttagtcgg catgatagcc aacatagcga tagcggttat
      901 catgacaatt ttcctcaacc gcaacatcac taaaccggtg aaaacaatcc aggtcgccgc
      961 ggacgcggta gcccagggag acctcacagt ctcagtgccg atcctcaaca caggcgacga
     1021 aatagccgac cttgctacat ccaccaggaa aatggttgaa aacctaacca atttgctgag
     1081 cgaaatgcaa aacgaagctg agcaggttgc gagcaccagc gaagagctct ccgcatccgg
     1141 agaggaatcg gcccacgctg cccagcagat ttccgcggca atccaggaga tggcctcagg
     1201 ggccgagcaa cagagcgcca atgccggcga gaccgccgca ggcgcgcagc aggtggctgc
     1261 agcaatcgag cagatagccg atggagcggg ccagcaaacg cagcaattgc agaacgtatc
     1321 ccgcctcata gcccaaatgg ggcaagagct cgaccatgta aatgacttcg ttatgaaaat
     1381 ggaagaagcc ataaatgcca cagcaaaaga ggccgaaaag ggagaccggt cagtcgccca
     1441 agtagccgcc agcatggaaa ggataaagaa ctcgtcaaac gaagtggagc aggcggcggc
     1501 agacctagag gcaagttcgc gccagatggt ccaggtggtg caggtaatag aggatatcgc
     1561 ggatcagacc aatcttctgg ctctcaacgc cgccatagaa gcggcgcgcg ccggcgaaca
     1621 gggccgcggt tttgcggtgg tagcggacga agtgcggaaa ctcgcagagg gctcgctaaa
     1681 tgaaacgaag gtcatctcgg gactgatcga gcaaacattg gcagaagtag gcaaagtggc
     1741 atcggccatc agaggcacaa acgatctcgt agaagagagc cttccactgg taatcgaatc
     1801 cacagaggtt ttgcataaca taagcaacca cgccgcgaac aacctagagc ttgcgcattc
     1861 agtagtcgaa tcgagcaaca gcttgatgga ggaagcgagc gatgtgaacg aggccactgt
     1921 ggaagttgta gcagtggctg acgagaacgc cgcggcatcc cagcagatgg cagcgagcat
     1981 acaacaagtg caaaaagcta tcgaaaacgt agctgccata tccgaagaaa acgcggcttc
     2041 agtagagaaa gtagcagcct caacggagca agtcaccgct tcgttgcagc agatatcctc
     2101 cgcttcccat tcccttgccg acatggcagc aaggctccag cagcttgcag ctaggttcaa
     2161 gcttgcatag atcgcatgag aaatgccctg acagctcagg gcatttctca tttcctattg
     2221 taactgcttc tctccgtcct gctaaaggac ctccgagact atgccaatcc gcacaaaaag
     2281 ccggattctt ctcatgagcg tcttcctccc taatatttcc ctcattcctg tcgatataat
     2341 tagtatgcag caagtaatgg gaggccaggc tatgaagttc aaaatacgag gcaagatcat
     2401 cttttcctac ataatcgtcc tcttggtcat gctaatcatg agcgtggcga acatccacgc
     2461 tgacagggta acgacaaagc actacgcaaa ccttgtcgag caaaaccttc ccctagaaac
     2521 gatagtttgg aaagttcgaa gcgagatctt ggaaaaaggc atagctttgc gcggctacgt
     2581 aatgacaggc aacccggcca ccatcgtcaa gttctacacc atagatgacg acgttctaca
     2641 gctcttcgat gaaatgcagc cgctgtgcga agataaggaa tctcagcgag cgttggagct
     2701 cctaaccgac ataaataatc gttacaacag cttcgcagac gatatttccg tctttgtcga
     2761 attcgaaatg atggatgagg cctatgcttt gatcgaagaa agagaagtat tcctcataga
     2821 cgaacttgac cagacaatcc ataattggat ccagctcata caggatcaga acagacagtc
     2881 aattgccaaa gcagagtccg cgagcagaat aaacgatcta atctccttag tcggcatgat
     2941 agccaacata gcaatagcgg ttgtaatgac aattctcctc aaccgcaata tcacgaaacc
     3001 ggtgaagaca atccaggtcg ccgcggacgc ggtagcccag ggagacctca cagtctcagt
     3061 gccgatcctc aacacaggcg acgaaatagc cgaccttgct acatccacca ggaaaatggt
     3121 tgaaaaccta accaatttgc tgagcgaaat gcaaaacgaa gctgagcagg ttgcgagcac
     3181 cagcgaagag ctctccgcat ccggagagga atcggcccac gctgcccagc agatttccgc
     3241 ggcaatccag gagatggcct caggggccga gcaacagagc gccaatgccg gcgagaccgc
     3301 cgcaggcgcg cagcaggtgg ctgcagcaat cgagcagata gccgatggag cgggccagca
     3361 aacgcagcaa ttgcagaacg tatcccgcct catagcccaa atggggcaag agctcgacca
     3421 tgtaaatgac ttcgttatga aaatggaaga agccataaat gccacagcaa aagaggccga
     3481 aaagggagac cggtcagtcg cccaagtagc cgccagcatg gaaaggataa agaactcgtc
     3541 aaacgaagtg gagcaggcgg cggcagacct agaggcaagt tcgcgccaga tggtccaggt
     3601 ggtgcaggta atagaggata tcgcggatca gaccaatctt ctggctctca acgccgccat
     3661 agaagcggcg cgcgccggcg aacagggccg cggttttgcg gtggtagcgg acgaagtgcg
     3721 gaaactcgca gagggctcgc taaatgaaac gaaggtcatc tcgggactga tcgagcaaac
     3781 attggcagaa gtaggcaaag tggcatcggc catcagaggc acaaacgatc tcgtagaaga
     3841 gagccttcca ctggtaatcg aatccacaga ggttttgcat aacataagca accacgccgc
     3901 gaacaaccta gagcttgcgc attcagtagt cgaatcgagc aacagcttga tggaggaagc
     3961 gagcgatgtg aacgaggcca ctgtggaagt tgtagcagtg gctgacgaga acgccgcggc
     4021 atcccagcag atggcagcga gcatacaaca agtgcaaaaa gctatcgaaa acgtagctgc
     4081 catatccgaa gaaaacgcgg cttcagtaga ggaagtggcg gcctcaacgg agcaaatcac
     4141 cgcttcgctg cagcagatgt cctctgcttc ccattccctt gccgacatgg cagcaaggct
     4201 acagcagctt gcagccaggt tcaagctagc atagatcgca tgagaaatgc cctgacagct
     4261 cagggcattt ctccattcaa atccgaaacc gttcccatga gagcgcggtc gccatggacc
     4321 gcgattacat ttacgcaagc ctggaccact tcaggatcgt acagtatacc acttttgctc
     4381 actatctcgt ccaatgctgc atccattccc aaagaaggcc tgtaaggcct gtgcgacgac
     4441 attgcctcca ccacgtcggc aactgcaatt atctttgccc cctcgcagat cgcgtccccg
     4501 gctaggccgg ctggataacc cgacccgtcc agcctctcgt gatgctgaag gaccatgttc
     4561 gcaatgggcc agggaaattc tatcgaattg accaccttaa aaccctcttc agggtgggcc
     4621 ttgatcaacg aaaactcaat tgggctcagt ttgcccggtt tagtcagtat ctcccccgga
     4681 accacgatct tgcctatgtc atgcaccagc gagccgaagt aaatcccgtc cacggcgtcc
     4741 ttggggtatc ccagttccat tgcgatgtgc cgtgccaggc aagccacgcg ttcctgatgg
     4801 cccgcggtat aagggtcgcg catctccaca acccgcgaca ttgcatggat ggctccctcc
     4861 gtagtctgcg cgagcttgac caggctggcc tcgagctgat cttgggctat cttgcgcaat
     4921 gtaatgtcct ccagagcaaa aaggacatag tgcggccctt ccaggctgac gctgaacaga
     4981 gtcgcaagca acctgcctac aaagaccgtg ccgtccatcc tcacataccg gctctccgct
     5041 gtaaaaaagc ttatcctgtc ccgcagaagc ctgctcacac gcgacgcaat atccgcttca
     5101 tcgtccgggt ggatcagttc ttctgccatg accccggcca agtcctccgg cagacatccg
     5161 aacagctccc caagagcggg gtttgcgtgg ataatccgca tatttgtgtc cgcaatcgcc
     5221 atcccaagct ggcttccctc gaaaactccc ctgaaccgca cctcgctgtc gcgcagcgcg
     5281 ttctccgccc gctttcgttg ggtgatatcc tcaaaaagag ccagtatgcc cttgagctcg
     5341 tccccctcgt aaagcgcggt aaagaaggca attgcatcta tcaattcccc gtccgcgcgc
     5401 cgacactgga cctcgattcc agatatggag tcgccggcaa cagccctcgc cagaatggct
     5461 gcagtctctt ccttgccagt ccatttcacc gcaggcatcg gtctgccgag agcgtccctt
     5521 ctcgcaacgc caaacacctc ttcagccgcc gggttccagc cacctatcac gataccccga
     5581 tcatcaacga gcatcagagc tatgggaatc gcttgcgcca aggtcttggc cttttctagt
     5641 ccctcaaagg ccaggctctt tgcagaccag tagttttctg cgctccttgt catcctcgcg
     5701 aatatgctcg gagtgatgac cccttttatc aagtagtcgc acgccccggc gcgaagcgcc
     5761 tcatactcca agtcctcctt gttgcgaggc acaacagcca ccaccggaat cgtaggaaac
     5821 tgccgcgcca tgagggtaac gtcatccagg gtaaagccgc ctccaggcga cacgtcaaca
     5881 aggacaatgg agagaacggg ctgccccaca tatcgggcca aggcttccgc agcccctgcc
     5941 cgagtactgc aagttatgat tctcatatcc gcgtctgaag gaaagggctc tctgcgcaat
     6001 gacacagcaa cccggattga agcctcgagc cgtggatcaa ggctcaaaac caccgctgtc
     6061 gaaggcagat tgatctccgc tttcaccctt cacccctccg tcttctgaag ataaagatga
     6121 atagcgccga ctctccccgg ctagtatatt gtaggcgcaa taaacacttt gagacaaccc
     6181 ttcccgagcc aacacggttc ggggcaggcg tcatccagac gatcttccat ccatatgaca
     6241 cattatgaac gagtttggaa ataataatat ggcaggaggt gcaacccaat tgcgctctcg
     6301 tgggaaacag cgctattatg ccgctattgt gcaagaagcg gcactcaagc gcagtagaga
     6361 agaaacaccg tcgtacgagg aggtctctaa tgtggacaat ggcacttacg aagagattgg
     6421 aaagaagtct ctagcagcat tctcaaatct actcgacatc ttgtcgggcg caaggacttc
     6481 gcctgccctt gccgatcccg taggtctgga aaggcgaatc ggagagcagc tggccaccgt
     6541 tgtggcatcc ctcgacttag tccgccgcga ccttgcggcc ttggccagca gaacagacag
     6601 ggctcttctc gagcttctga agagaatcga acctcaggaa ggattggcag tcacatcatt
     6661 tcccacaccc agcctccctg cctcacaggt ttcagccatc ggcaccgcgg gtcccggaac
     6721 tgggggacgc gctgcgaccc cgtcgaaacc gcgagggaag tatgagagag tgcccaaaga
     6781 acagacgaga gagaagatac tcgaagccgc cagagaactt gcggctgccg gccgaaagat
     6841 aacccttgca gaatgcgccc gcatggcgga tgtggcctat tacaaggccg tgtatgcctg
     6901 caaagactct gaggaactcg aggagctgat gagcggaaac gctggctgat gagtggagac
     6961 aaagctgact gcggcacgac gcccttccct gctctaaatc gcgcagggaa agagaccacc
     7021 gatgccgcag tcctgctgct ctgttcttgt gcgaggcgga aatgctgatc atcatcgatc
     7081 aggagccaaa gcggcaccca caatcggctc tatagacacg tccgggtttc cgctcgggct
     7141 tcagaatcca gcacgcgatt atcgctatca acatcaacgc ggtcacggcg ccaatatgtg
     7201 acgaccattt cgcggaagac cctgccgcgc gccttctgat aaaagctcat ctccaacctt
     7261 tcctgtcatc ttttgtgtgg tgaaggatat cagtccttgc aacgggtccg tcctttcttt
     7321 taacaggtgc ggcgggacgc atcaccaacg ccatccgccc accgtggcgg gcggatggcg
     7381 cgtgggccgt tgtccaagct cgatataggc gatgagctgt tttgccagcg tggggacgag
     7441 ctggttctta ccagcaaagc ataaaatggc aaatgcagca cacgatgaat gcgattacca
     7501 ctacccaaaa ccaaatccac caaggaaaac agcactggct ggcagtcact tcggccacat
     7561 ccggtcacct cctgatgcat gtctggtcca tactatgacg cctgaagcga acttgatctt
     7621 acttgcgcgg aggaaaatcg tgccttacca gcaaagcacg aaacggcaga tgcagcacac
     7681 gatgaatgcg atgatcgcaa cccaaagcca gaaccaccaa gggcagaagc actggctggc
     7741 agtcacgtcg gccacgtccg gtcacctcct gaggtaggtc tggcttattc tatgttgccc
     7801 cggataaaat ggaaccgggc tcaatgtcag ccatgttgcg atttgctatg tttcttcacg
     7861 ccggcgccag cttgatatcg ccccatgccc ccaaggcatc gccgatgacg ataacagccc
     7921 cgactatgcc gggaatcttc gaagccgtgt ccaacgcctc ttcaatgtcc tccctcgatt
     7981 tcacgacatt tgcgacccta gtggcaacgg catcggcgag ggctgtgtct ttcgcaacga
     8041 tcacggctcc gtcgcatcta ccgaaactgc gcgaatgccc gaccgttcca gaagacgtac
     8101 agatgccgca aggggtctgg cccggagcaa tctccagggc aatcttgcca ctcaaagggg
     8161 acgtgcccgc gtagattcca accctgcgca aacggctgga ggcgatgaac acgtctcccc
     8221 cattctccac tatcacttca cttatcctcg ctcgatgccc atcaggattc ccaatgtcag
     8281 gctcgcgctg ccacagctta gaaaggctat cctccgacag gaggctgatc ccaaccgtct
     8341 cgcttatggc gccggccacg gcggccatgg gccccacccc tgcctgcgca gcacaggcgc
     8401 acatccatct gacaataggc ggcgcccatg gccgcggaat caccgggatg aggctggtga
     8461 gaaactcggg gtgagtggcg atgtagtttt cgatatctct gcgggcgcga agaacaacgc
     8521 ccgagacatg atcccgaaga aagctctctt cgggaagttt cccgcgaaaa tcagggcaag
     8581 agaagccaac gccgacttga agatcagtct gccccacggc gcatctgaaa tagattacat
     8641 cttgagcgct gaaaagctcc ctgtatttcc tcggttcgta ggcttctctc acgcaacttt
     8701 cccccaacct atctttgccc atcccgcccc ctctatatgg gcgagctcat tgcccccacg
     8761 ggacatgtgc gggcgcacaa ccggcaaaga tagcatttct ctttgtcaaa tacaaggctc
     8821 caatcacagt tatccatagt gagcgcgtgc gaagggcata cagccgtgca tgctccacaa
     8881 tgcacgcact tgtcctcatc ccaaacgatg ctgccattta ggatctcgta cccaatgccc
     8941 tcttcgtcga ggaactccaa gccccgatcc acatcttcaa cgcttccctg tatgtccatg
     9001 atgagcttgc cggctttgtc cgcggcgatt tcggcgtgga gaatattgat ccacagatcg
     9061 aactcgcgga tcagggagta agttatgggc ttgcctgccg cgcccggagg gtaggtcagc
     9121 aacgccttaa ctctctttgc cgtcgtcgca tcagttatgc catcggcccc aacagtcctc
     9181 tccacggtca tccttactcc ccctcacagt tatctagtgt agtcggcgag ccttcgagcg
     9241 gaagagccgc cacaggctgc tgcaacagga actctcctcg ggcaatccag cctttcaacg
     9301 tctgggctat ctcccgcgcc accttcaggc tggaaagagg cgcggtaggg atcgtgcgcg
     9361 acccaatctg aacactcccg ctccgcagtt cctcgtagga gacttcggcc aatgcaggtt
     9421 tggatcgacg accgacgctg taatcaaaga ccttcgtctt tatgtcgcgg ttttgcaccg
     9481 cgacgaatct gaccatctcc tcgtccagca gaggaatggc agtacccacc ccgacaaaca
     9541 tggtaacccc atacttttcg aacctggcgg cccttatgta tctcggatcc atctgtttta
     9601 ggtcgcctat aagagcgagg catgccgctc cgccgatagg aaccccgttt tccgcccgag
     9661 gctgcgacgg gttgttctgg gttccctgcc acgcaacgta cccttgggcg cctccgagga
     9721 atatccgtga acccaacccg atcgtgcggt agaacgggtc gtttatcaaa gggctgagct
     9781 cgcctgcagt gcagtagttc acattcccaa agtggggcag gagtgttccc atgtacgtat
     9841 aaatagtgcg gtccgaggaa ttggtcgccg ccccgtaatt ctggtagcag ttgcgggggt
     9901 tgaacatgta ggcctcattg attgaatcct tggtaatcca tgcctcgagc gcgcgccgag
     9961 gataacagtc tgtcccgtag gaagtggcct taagcctcac ctttctgcct gctatgaggt
    10021 cttcgatgac gtgcgctccc ccgtattcca tccccgcggt ctccgagagc tccgttgcac
    10081 cgatataggc gtctacagca gcaagcccag cgcatgccgg cacgtcattt agccacacgc
    10141 ggctcatcct tatgggagga tcagagtggc cgaagttgag aaaagcccct gaggaacaca
    10201 tcgggctgaa agtgccgcaa gtcacgacat ccacgtcgcg aaaggccccc tctaccccct
    10261 tctcgtccac aatctctatt atctcctcag cagtcacaac aaccgccttt ttgctgcgta
    10321 tcttctcgtt tatctcttca aagctcctgt agcccatcta ctctttccct cccacttgat
    10381 cgctttgccg cccaaaagca cgctttcttg ccaaaagcct ttctacctcg aagaccgctt
    10441 ccacggcatc actgccgtat ccgtccgctc ctatgcgtat ggcgtactcc cgtgatgtag
    10501 ctgcgcctcc aatgatgatt gggcaatcgt acccctcgct tgaaaaaagc tctatcgact
    10561 cgcgcatctt cggagccgtc gttgtcatca gcgcgctcag gcagaccacg tcggccccgg
    10621 ccttcttggc ccctgccaga acatccctgg ccttaacgtt ctttcccagg tctactacgt
    10681 tgtagccgtt gctttcgagg aacatggaga cgatgttctt gcctatgtcg tggatatccc
    10741 cttcgactgt ggcaatcacc acagtccctc gggatgccgg cgcatcctct gcgctgccta
    10801 ccctggacct gataacttca gttgcagcct gcatcgcccc tgccgacagc atcagttcag
    10861 ggaggaagta agcgcctgag gcgaagcgcc gtccggtctc ttctatagcc gggatgagcg
    10921 cgtcgtttag gatagaagac ggatcctctc cctccgaaac cgcctcctcc acaaacctta
    10981 cggcgaggtc cttgtcgccc ccgaggaccg catcatagat caaggtcaac ttcttcttcc
    11041 cgcctggatc ggcagcaacg ccatgaggtg caggcgacgc cgcgcccgcc cacaagatgt
    11101 atctttccgc attaacatcc cgcccgagaa gaacccttgc cgcccgtacc tgagccatca
    11161 tcgcctcatc aagggggtta gctatagcca tgtctaaccc ggcagcgatg gccagcatca
    11221 agaatgcagt gttcaacacg ggcttgctcg gaagcccgaa cgacacattg gaaatcccca
    11281 gagaagtctt gcagcccagc tctttgcgga tcatctctat ggccttgaga gtctccacag
    11341 cctgttcctg ctttgccccg caaggcatcg ccaaagcgtc gatgatcacc gcattttggg
    11401 ggattccatg agattgtgcg ctcgagacga gccgctcagc cagtctcagc cggtcctctg
    11461 ccgtcgccgg aatcccgttc tcgtcgatag gcaagcctat caccgccgcg ccgtatctgg
    11521 ctgcaagcct cagcacgtcc tcttccctgc cacgctccag cgagaaggag ttgaccaagg
    11581 ctcggcctgc ataagcccga agccctgctt cgagagcctc ggcagatgaa gagtcgaggc
    11641 agagcggcag acgagtggtc gcctcaatcg ccttcactgc ttcatacatc gcccacggct
    11701 cgttcgcaag aggcgtgccc acattcacat cgagagcttg cgctcccgcc ttagcctgag
    11761 caaccgcttc cttccgaact aactcgaagc tgccgtcgac aatgtcttgg gcaagtttgc
    11821 gcctgccagt tgggttaatc ctctcgccca caatcgcggg cagctccgca tcggcgaaaa
    11881 acagaggctg agtccgggaa gttataccaa gctcaggctc gcgccttcgc acgatcggcc
    11941 ggagcccctc cagcgcactg cgaagccttg cgatgtgctc cggggtagtt ccgcagcagc
    12001 cccctacgat agaggctccc gccgacacca gcttagggcc gtaggacgcg aattcctcag
    12061 gccccattgg atactcgccc cgccccgatg ggcccacatg cggcagtcca gcattgggct
    12121 ggaccgatat tggctttgat gtaacagccc ccatccgagc tacaacatct gcgagcaggt
    12181 ccgggccgac agagcagttg acgcccacga cagacgcccc catcgactcc atgacgagcg
    12241 cggccgactc cggcggagtg ccggtgaagg ttcggcctgt ggaatcgaat gtcatctggg
    12301 cgattaccgg gatgtctggc gcacgatcgc ggcacgcgag gagcgctgcg cgcatctcgt
    12361 tgagatcggc aatggtctcg atgatgataa ggtccggtcg cgccagggca aacgcctccg
    12421 cctgctccgc aaattgcaga taggcttcat cgaaactgac ttcgcccaaa ggctccacca
    12481 atgcccccag ggggcccatg gaagccgcca caagcgttcc gcggtcggca gcagccctcc
    12541 gggcaatctc gacggcagcc aggttgatct cccgcgccgc atgagctagc ccgaaatgtc
    12601 cgagccgcaa agctgtgccc ccgaaagtgc aagtctcgat cacctgcgcg ccggcatcaa
    12661 tatactggcg gtgaacccgc tccacttctt ccgggcactt taggcaccac acttcaggac
    12721 aggctccggg aggcagaccg cgcgcctgaa gcatcgtccc aaatgccccg tcaaacacga
    12781 acacctcgtc cccacggatc agatcctcca cacgcacact catccgttcg gagccacctt
    12841 ccgcaggccg tcgcatgccg tttgtaccgg gggcaccgca aacccctcct cggccaaggc
    12901 gtccagcacg cctacaaccc gcttggaact gttcatgggg aagatgtgta tcccggcgat
    12961 atccttccct atctccgata gcaactccgc cgccagcctg tacccttcac cgtctccccc
    13021 ggagcgcatc cggtcgataa cccagtcggg cacctccatg ccaggaacat tctcctttag
    13081 gtgcacagca aaggcagcat ctttgatagg gagtatcccg tagaggatag ggatgctcac
    13141 ccgcccgtct atggcattag cccacctttc ataggcgcgg acgtcgaaaa tgggctgggt
    13201 ctggacgaaa tgcgctccag ctgcgatctt ctcctccagc ctcgatatct cggcctcaag
    13261 atcttcggcg ttgggatttg ctacgcaacc gatggcgaaa tcggtccctt cacccagctc
    13321 gccgccgcct accgtgtgtc ccgagttgag cgcggcggcc aagctgatta gcccaacagc
    13381 atctacctcg aacactccat gggcctccgg atgatccccc ctcgacggcg gatcgcccct
    13441 gagggccagt atgttcctga ctccgagccc ggaggcaccc aaaagctccg cctgaagccc
    13501 tatggtgttg cgatcacggc acgtaaggtg gaaaatggcc tctaccccta catcgcgctg
    13561 gataaggtgg gctaaggtaa tagggctcat cctcacattt gccatagggc agtccgccac
    13621 attcacggcg tccacccggt cagaaaaccg gcgaaccagc gcaagcgtct tggaaacatc
    13681 cgcacctttc ggggggttaa cctccaccgt aactgcaaag ctgccctgcc tcaacctgtt
    13741 caataatctt gccacaaaaa ctccctcctg cgcgagcttt ctctacttat ctctgctgaa
    13801 aagtctttag ccgcccaagc caagaagggc aaatgcaaaa agccctctcc cccaggcaaa
    13861 atgcctggga cgagaggact aatccttcgt ggtaccaccc aggttcacgc ggttctcacg
    13921 ataccgcgcc tcggcaggtg cggcgaatcc atccgattcg ctagaccccg gcgctttaac
    13981 gtgcgcacac gtccgcgcct actttccctc cctgcccatc aaggagcaaa gcggaggaag
    14041 ttcggacggc cgctcagggg ccatgttcgt cgcatttccg cggttccggc tttcagctcg
    14101 aggccggctc tctgtgcccg gtactgtgcg agtactcatc cccgtcattg cgtttagctg
    14161 cgattttaaa cttggagaca gtatataccc ccgtgtagcg gatgtcaagg ttgggttttc
    14221 aatgaggcat ttgcgttgta gccgatttct cgcgcatgcc agttgcataa tttgtc
//
LOCUS       DAOSYL010000062        14141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3620_length_14141_cov_7.339415, whole genome shotgun sequence.
ACCESSION   DAOSYL010000062 DAOSYL010000000
VERSION     DAOSYL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_14141_cov_7.339415"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLU15_08995"
     CDS             complement(<1..306)
                     /locus_tag="PLU15_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOO31094.1"
                     /translation="MHDFSLMGIDIGTSAVKVLLWRSDGTHRAAYRRAKESYVTGRGV
                     GVAASEQDPAALWRIVTQCIREALQGWDAGSVAGVGLSGHGPSLMAINRGGDPLSNII
                     "
     gene            complement(299..1339)
                     /locus_tag="PLU15_09000"
     CDS             complement(299..1339)
                     /locus_tag="PLU15_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO31095.1"
                     /translation="MKAVVYHGPGDLRVENVDDPVLEEGDVLLGVSYCGLCGTDVKTY
                     KRGHHMFTPPCILGHEVVGRIVEIVGEPVKPDIAVGDMVAVAPYVPCYGCAYCRSGRE
                     ELCTNKGFIEGAFAEYVRIPAEVVSKGTFHIPSEEDIKLACLAEPLACCVNAAQDSHV
                     RLADTVMIIGAGPMGLLMLELCKAMGASKILVSEPNGFRRNKAAERGAVVIDPACSGT
                     DVSAWAKEQTDGIGADVVFVCVGATEAVDAALGCVRKGGTVNIFGGLPSGSRLTVDPH
                     ILHYDEVILTGSFGFTPLQFHTALTMLTGGAIDAGSIITHEFDVDQAVEAFDMASSGE
                     SLKILLKVDGDA"
     gene            complement(1342..2100)
                     /locus_tag="PLU15_09005"
     CDS             complement(1342..2100)
                     /locus_tag="PLU15_09005"
                     /inference="COORDINATES: protein motif:HMM:NF005556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOO31096.1"
                     /translation="MAGIDVRLRRIFGEDGKAVICPLDFGGFMGPVKGLYDPSAAVRK
                     VVEGGCDAILVNPGLAATEWESFAGKTGLVVRVTGGSTKFSPNPAFHTLVCSVEEACR
                     LGADAICIMLLVGSDYEQEMFEIMGQVVSEAHVLGIPVLAEVIPCDPAHSFDPEWISV
                     CARVGYELGADAIKTYCTAEGFEEIVRACRVPIVIAGGPKVEDPNSVVRRAMSAGAAG
                     IAFGRNVFQADDPVAKVKELYSIVHGDCSCKEGR"
     gene            complement(2081..3109)
                     /locus_tag="PLU15_09010"
     CDS             complement(2081..3109)
                     /locus_tag="PLU15_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOO31097.1"
                     /translation="MQRGSLDPSRVAGTLAGVAIGDAMGMPSEFMTREEIRDVYGRIE
                     GFCVPHEGHIHAGMAGGCITDDTEQTIAIIEALERHDRITPEVAAAAYLRWAQQCNAY
                     ESSVLGPSSRRALERLKAGEEPRVTGSSGNTVGAAMRVAPIGIVNSPNLESAAEECYM
                     SCLPTHGVNIAIAGASAICCGVAAALVAESLDEVIDATVFGARHGERLGIEWAGTLVS
                     ARIELALRIVRESRDAVEAEDQLYTTCGVGMDPTELVPTAIGLFALHEGDPQRTVPAA
                     ANMGGDTDTLASMAGALSGAYRGIGAFPEEWVRTVEEVNNLNFEELTVILVAVRERRR
                     VNNGWNRC"
     gene            complement(3096..4127)
                     /locus_tag="PLU15_09015"
     CDS             complement(3096..4127)
                     /locus_tag="PLU15_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOO31098.1"
                     /translation="MGRTDGVVVSLGAAAMDMVFSVSKLPEPDEMVFAEGEVCFFPGG
                     STANIAAGIGRLGYRSRFVGRVGNDDNGRRLRRAFEADGVDVRWLAEIEGGRTAQTII
                     AVDRRGERVIYSLGGEAILQEPDEFEDVAMEGAEILYIGEVLPDVAARAIQLARLYGV
                     TVVYGPGGGISWIDSATLYQLLKECDYVLLSRGELDAVTSCPSHTEGAGRLLESGVRN
                     VIVTLGSDGSECHSRGAGRSPWYGSSWNGRAGDVAHDMVNGSSNGRGTNTVLRAESFR
                     VTNVVDTTGAGDSFAAGFITGLLRGFDIVECLERGNACAALAIQKTGAREAMPTSEEL
                     EEFLRYAER"
     gene            complement(4129..4962)
                     /locus_tag="PLU15_09020"
     CDS             complement(4129..4962)
                     /locus_tag="PLU15_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO31099.1"
                     /translation="MIVEYESKQTSIHNLDPRAKFIWLIATIVISIIWTNPVYLAALG
                     LVTVVIGCMGEFPWAKMKGTFLFLLVITAIITLVQGATYVPKAANLAYPERVLFHLVP
                     GWIPGVNPAAPIRIGGFLYGVGMALKVFVVLIVISVFGYVTSPSEVVQIIVRIPFFSY
                     KSGFVVSAASKFVPVIQMQMATLRDAERSKGVDFDSGSFTQKVSKTTSMLIPLFANAL
                     SMADTMALAMESRAFGYSENFTLIRPYHMHWPDWLMAVLSIGALAASIVLLCVYKMGA
                     L"
     gene            complement(4959..6734)
                     /locus_tag="PLU15_09025"
     CDS             complement(4959..6734)
                     /locus_tag="PLU15_09025"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOO31100.1"
                     /translation="MAPTIVSLKDVTFAYKGSKRQAIKGVSLDVKEGECVAILGPTGA
                     GKSTLACVINGTVPRFLEGELTGEVVVDGQVPNEVGTAQMASKVGLVFGDPDTQLFGM
                     TVEEDVAFGPANLGFDYDTIMGRVNRAIEDMRLKGLEQRAPHRLSGGQKQATAIAGVY
                     AMLPKIMVLDEPTSMLDPEGKARVFSIVRYLNKSLGMTIILIEQEVDDILQLADRVYV
                     MKSGKFVFGGTPREVFGSVNDLKSAGVRVPHIVEFGDLIGSAEAPFSVEEAISVVERG
                     AWQPAASVETGLADDQSISGSSGNKMQHGALTTSFDGVSPAVEVIDLEFKYPMGAKAL
                     NGVNLKVDSGEFVAIVGQNGAGKTTLTRHLNGLLRPSSGDIRLFGESILNESTTKLCP
                     MVGYVFQNPDEQLFCTTVEDELRFGPSNIGMDKDRMEQAVRDILLDIGLDKYRQVWPR
                     YLTKGERQRLALASVVAMEPRILIVDEPTTGLDWLESLQILDYLEKLRKEKGKTILII
                     THDMNIVSLYARRVVVMANGRVIGEGTPREIFSQPQLMEQAYLRRPAIADLSDALWGE
                     VCLTPQEAAYRLLESSRTNAEVSQK"
     gene            complement(6747..7397)
                     /locus_tag="PLU15_09030"
     CDS             complement(6747..7397)
                     /locus_tag="PLU15_09030"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO31101.1"
                     /translation="MPLGQGAVGLGVSYALAICYLLLMKLAGDILTKQYPEEHKVVWD
                     TRTISRVTILAAVSGAGAMIKIPGPATTIALDAAAGYFAASYFGWPIGATVAAIGCFL
                     ANALGGFASWLPMVPIYVCAMAFTVTIGVFVAKKTNIVLAIIVGTVVNTITCLGPWVV
                     MIGPALMVALLPSQLIGSAVNAAVGLTVAQALKSAQTHEKSDVEELVEPEDESFKM"
     gene            complement(7519..8466)
                     /locus_tag="PLU15_09035"
     CDS             complement(7519..8466)
                     /locus_tag="PLU15_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOO31102.1"
                     /translation="MAFDKRLLYEIAKNYYELDKTQSEIASSLGISRSQVSRALKQAK
                     DEGIVVVKLIPPGLDFADLEEKLRSTFGLSDAVVISGKAGPSRLLTQNLGMAGANYLD
                     RELRPGQVIGVSWGATLRELVAALGSIKPEPREIMAIPLLGGQGQASPDLQVNDIASR
                     VAMAFGGSHLYLHAPSFVDTPEARNMMMNDSNIRNVASKWAAADVAVVGVGCFVPPST
                     LLEEGGFPPDELEDLVRMGVVGDICMRFFDEQGQPVDTPLGDRIIGASLDQLRAIDCV
                     VAVAGGVNKAASILGALRTGVVDVLITDDLTARRVLEIS"
     gene            complement(9230..9820)
                     /locus_tag="PLU15_09040"
     CDS             complement(9230..9820)
                     /locus_tag="PLU15_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO31103.1"
                     /translation="MTQCYGMGTRGIICRTAVRVFAEKGYDAASMDQIALEAGVAKGT
                     VYYHFKSKSNLFSAIIENGLDALIEDVEGATLRSTDPVEQLSAIFDALIDYAQTFDDF
                     MYLLFKEALAPGREWASDVVVYWHRFVDIIEDVVARGKSMGRIDNVDTDAITQSIVSS
                     LATAALGWATGIGDEMWAERVLHGVKGITLRGIVVA"
     gene            complement(9827..11863)
                     /locus_tag="PLU15_09045"
     CDS             complement(9827..11863)
                     /locus_tag="PLU15_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31104.1"
                     /translation="MKNRTAKITAVIGACAILMLCSVGMAQSPGGVSETSGTSGTYGR
                     IQEYETVFCVLASDGMPESTTVVDWIRVPQGGPVSVFDPGNIENPVNLRNAEVPDVVS
                     GGLKWNADPTRITDINYSGTSKKQLPIGVEVTYKLNGKVVPAQAVPGSSGRLEVTVDL
                     VNRTARKENLSYTVDGKNTTFAEDVCVPMIVQVSTDVPLPDYTLIQAPDATTVLAGKS
                     LKINWTLMPNPDASCTLVLEGENIQLNDIDVTVIPSMPPIPQLEELLDTAIDALGMLA
                     SGVDQLDSAIEQAQSGTLKLADGQAQVADGMIAIGNGVSDLSKLADAHWTIAKTMNDA
                     ITPEMLEKPKQLIELVESAKPILVKLSDTISEAMSSLPMDQLGTAAELAPKLLEQATE
                     LNDKAQRVSKGLSSCSQTITQAKSASSESMAALEALAKSNPEITKSLQYKKLESSIKK
                     QDALLETAKSGGRVGFSQVPSIDELAEDAKSVASVGSKAKIAISLASSQLSKVSLDSV
                     DDYLNQAMGAVEVLNALLYGGTVQGHEVPSIDEICDNLRKLDEGAHDLKTGLSLLAVG
                     GTVDGQNVPGLDTAVSGLEALVDGISKLSNGTVELTAGTKELASGLGRIRNEGTSEMK
                     KGINEGLAEALKAKAQLDAMKVRLDQYDTFEGKPAGAAGEVRFLMKVKAPSNKG"
     gene            complement(11886..13994)
                     /locus_tag="PLU15_09050"
     CDS             complement(11886..13994)
                     /locus_tag="PLU15_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO31105.1"
                     /translation="MKGLAEFVVKNRVAILIIAAVLLVPSVYGMQHRKVQYDLLTYLP
                     KDLNSVEGMNVLDEEFKYAERAFVVCRNQPDWAVLKVKEKLEHVDGVSSVFWLSDLVD
                     PTIPHDYLGEDIVSKFYRGQITLLIVNFEEDAVSPRTERAVNDIKNLLDKEQWVIGAL
                     VSGLELKDLGVEQTPVMVRTAVLLILILLFLTLPSPLMPLVFMGTIGVAYAYNMGINY
                     LLGRDVSYVTNAVASAMQLGVTMDYCIFLLHTFQTECEKHDSEEAMVRAITYTANAVL
                     SSATTTAVGFGSLALMRITLGADMGILMARGVLLSVICCLTILPSLVLTLNPILGKWN
                     HPVILPSYKGLGDWLTEQRVPVFALTVVLALVGLYGYNKIEPSYDIESTFPMDLPSIA
                     ALQEFKDDLGSVEEAYIITRGVPQWRLQELTRQIREMDGVKDASCLGDVVDPGIPVEF
                     VPEGVSKQYVGGEYENTVITLEGSILDPRTPEFITQTRELIKDLPGEVYFTGTSVIGK
                     DLEDLSSKDLDLVNWVSMAGIFAIVAIAFKSWTVPILLILAIKVAIWVNQAISYFTGA
                     PVFFFSRMAIEAIQLGATVDYAILVTNTFKGKLNKNAPPDAMRSTVAECGPAVLNSGL
                     AMFCAIIGVYASTNLRAIKDLALLLARGALVSVAVTSFMLPATLVVCNPILEKTSIGW
                     PKHDSVRQAGTSAVAASDDK"
BASE COUNT         3250 a         4341 c         3294 g         3256 t
ORIGIN      
        1 gataatgttg ctgagtggat ctccgcctct gttgatcgcc ataagcgacg gcccatgtcc
       61 tgacagaccc acgccggcca cgctgcccgc atcccatccc tgcaacgcct ctcgtatgca
      121 ctgagtcaca atgcgccaca gcgcagcagg atcctgttca gaggcagcca cgcccacacc
      181 ccggcccgtt acatatgatt ccttggcgcg acggtatgcc gcccgatggg tgccgtctga
      241 acgccacagc aacaccttga ctgctgaggt tcctatgtct atccccatga gagaaaagtc
      301 atgcatcgcc atcaaccttc aggagtattt tcaaagattc ccctgaggac gccatatcaa
      361 aagcttctac cgcctgatcc acatcgaatt cgtgcgtgat gatgctcccc gcatctatcg
      421 cgcctcctgt cagcatggtc aatgccgtgt gaaactgcag gggcgtgaag ccgaaactcc
      481 cggtaagaat gacttcgtca taatgaagga tgtgcggatc tacagttagc cgagacccac
      541 ttggcagtcc gccgaaaatg ttgactgttc ctccctttct gacacatcca agagccgcat
      601 ccactgcctc agtagctcca acgcagacaa ataccacgtc agcaccgatc ccgtccgtct
      661 gttcctttgc ccatgccgaa acgtccgttc ctgaacatgc aggatctata accaccgccc
      721 ctctttcagc agccttattc cttctgaaac cattgggctc gctcaccaga atcttcgaag
      781 ctcccattgc cttacacagc tcgagcatta ggaggcccat tggccctgct ccaattatca
      841 tgaccgtatc cgccagcctc acatgagagt cctgcgcagc attcacacag caggcaagtg
      901 gttcagcaag gcacgccagt ttgatgtctt cttctgacgg aatgtggaac gtccccttgc
      961 tgacgacttc ggcaggaatc cttacgtact ccgcaaaggc cccctcgata aagcccttgt
     1021 tagtgcaaag ttcctctctg ccactgcggc aataggcgca gccgtagcat gggacatacg
     1081 gggcaactgc taccatatcg ccaacggcaa tgtctggctt tacaggctcg cctacgatct
     1141 ccactattct tccgaccact tcatggccga ggatgcacgg aggggtaaac atgtggtgcc
     1201 cgcgcttgta tgtcttgaca tccgtcccgc agagcccgca ataggagacc ccaagcagca
     1261 cgtcgccctc ctcgagaacc ggatcatcta cgttctccac acgaagatcg ccagggccgt
     1321 ggtacacaac tgctttcacc gctatcgtcc ctccttgcag gagcaatctc cgtggacaat
     1381 gctgtatagc tctttgacct ttgctaccgg atcatcagcc tggaacacgt tgcgaccaaa
     1441 agctattccc gcagccccag ctgacatggc gcggcgaact acactattgg gatcctctac
     1501 cttaggaccg cctgctatga caatcgggac tctgcacgcc ctgactatct cctcaaaacc
     1561 ctcggctgtg cagtatgtct ttatcgcatc cgcgcccagt tcgtatccga ctcgtgcgca
     1621 cacgcttatc cattctggat cgaaactgtg cgccggatcg cacgggataa cctcggcaag
     1681 aacgggaatc ccgagaacgt gtgcctcaga gactacctgg cccatgattt cgaacatttc
     1741 ctgctcataa tcggaaccaa ccaaaagcat gatgcagatg gcgtcggcgc caagccgaca
     1801 tgcttcctcc accgagcaga ccagcgtgtg gaatgcgggg ttcggactga atttggtgga
     1861 gcctcccgtc accctgacca caaggcccgt cttccctgcg aagctctccc attccgtggc
     1921 ggctaagccc ggattgacca gtatggcgtc gcaccctcct tcgaccactt ttctgactgc
     1981 cgcagaggga tcatacagcc ccttgaccgg tcccatgaaa cctccaaaat cgagcgggca
     2041 tatgaccgcc ttcccgtcct ctccgaatat ccgccgaagc ctaacatcta ttccagccat
     2101 tattgacgcg tctcctttcc cttacagcca caagaatcac cgtgagttct tcaaaattca
     2161 ggttgttgac ttcttcgaca gtacgcaccc attcttccgg gaacgcccca atgccgcggt
     2221 aagcgcctga caaggccccc gccatcgatg caagagtatc tgtgtcgcct cccatgtttg
     2281 cggccgctgg cacagtcctc tgcggatcgc cttcgtgcag agcgaagaga cctatggccg
     2341 tcggcaccag ctctgtggga tccattccaa ccccgcacgt cgtgtaaagc tggtcctccg
     2401 cctctaccgc atccctgctc tcgcgcacaa tcctgagtgc gagttcaata cgagcgctca
     2461 ccagtgtacc cgcccactcg attccgaggc gttccccatg ccgggcgcca aacacggtgg
     2521 catcgatcac ctcgtccagc gactctgcaa caagagcagc cgctacaccg cagcagattg
     2581 cagacgcccc tgcaatagct atgttcacac cgtgcgtcgg caggcacgac atgtagcact
     2641 cttcagccgc actttccaag ttgggggaat tgactatccc aattggcgca acgcgcatag
     2701 cagctccgac ggtgttcccg gatgaccccg tcacccgagg ttcttcaccc gccttaagcc
     2761 tctccaaagc tctgcgcgaa ctaggcccca gcaccgatga ttcgtaggcg ttacactgct
     2821 gggcccatcg caaatatgcg gcggcagcaa cctccggagt tatcctatcg tgcctttcca
     2881 gggcctcgat gatcgcgatc gtctgttccg tgtcgtcggt gatgcaacct ccggccatgc
     2941 cggcgtggat gtgtccttca tggggaacgc agaatccctc tatcctccca tatacatcgc
     3001 gaatctcttc acgcgtcatg aactccgacg gcattcccat tgcgtcccct atggcaactc
     3061 ccgcaagggt gcctgctact ctgcttggat ccaagctacc tctctgcata acgcaggaac
     3121 tcctccaatt cttcactagt gggcatcgcc tccctggccc cagttttctg gatggccagc
     3181 gctgcgcagg cattgccccg ctcaaggcac tcgactatat caaagccccg cagcagacct
     3241 gtgatgaatc ccgcggcaaa agaatcaccg gccccagttg tgtcgacaac attggtaaca
     3301 cggaaggact ctgcgcgcaa tacagtgttg gtacctctac cattgctcga gccgttcacc
     3361 atgtcatggg ctacgtcgcc tgcccgacca ttccaagagc tgccatacca aggacttcgt
     3421 ccagccccgc gggagtggca ttccgatcca tcggagccca gggtaacaat gacgttgcgg
     3481 acgccgctct caagcagccg ccctgcaccc tcggtatgcg agggacacga tgtgacggca
     3541 tcgagctctc cgcgggagag cagaacatag tcacattcct tcagcagctg atagagtgtc
     3601 gcgctgtcga tccatgagat cccgcctccg gggccataga cgacagtgac gccatacaac
     3661 ctcgcaagtt ggatggcccg agcagctacg tccggcaaca cctcaccgat gtaaaggatc
     3721 tccgcaccct ccatggccac gtcctcaaac tcatccggtt cctgcaaaat agcctcgccg
     3781 ccaagggaat agatgaccct ttcgcccctt ctatccacag caattatggt ctgggcggtc
     3841 ctacccccct cgatctccgc aagccacctc acatccacac catcagcttc aaaagcccgc
     3901 cgcaatctac ggccattgtc gtcattacca accctgccaa cgaacctaga ccgatagcca
     3961 agccgaccga ttcctgctgc aatattcgca gttgaccctc ctgggaaaaa gcagacctcc
     4021 ccttcagcaa ataccatctc atccggctcg ggcagcttac tcacggaaaa caccatgtcc
     4081 atcgcagcag cgccgagaga gactacaaca ccatccgttc ggcccattct agagcgcccc
     4141 cattttgtac acgcaaagca gtactatgct tgccgctaag gccccgattg acaagacagc
     4201 catcaaccaa tcaggccaat gcatgtggta cggcctgatc aatgtgaagt tctcgctgta
     4261 tccaaatgcc cgagattcca tggctaatgc catcgtatca gccatagaaa gcgcgttcgc
     4321 gaacagcgga atcagcatgc tagtggtctt gctcaccttc tgggtgaaag atcctgaatc
     4381 gaagtcaacg cctttcgatc tctccgcgtc cctcaaggtg gccatctgca tctggatcac
     4441 cggcacgaac tttgaggccg ctgagaccac gaatccagat ttgtaggaaa aaaacgggat
     4501 gcgcacaatg atctgcacta cttccgacgg cgatgttaca tatccaaata ccgatattac
     4561 aataaggacc acgaatactt tgagcgccat cccgactcca tagaggaatc cgccaatccg
     4621 tatcggggct gccgggttaa ccccaggaat ccagcctggc accaagtgga acaacacccg
     4681 ctccgggtaa gcaaggttgg cagctttggg tacatacgta gccccctgaa ccagcgtgat
     4741 tattgccgtg atgacgagca ggaacaaaaa ggtccctttc atcttcgccc aggggaattc
     4801 tcccatacag ccaatgacga cagtcaccaa ccccagtgca gccaaataga ccgggttggt
     4861 ccagatgatg cttatgacta tcgtggcaat caaccagatg aacttggctc tgggatcgag
     4921 attgtgtata gacgtttgct tgctttcgta ctcgacaatc acttttggga cacctctgca
     4981 ttcgtccttg atgattctaa gagcctatac gccgcctctt gcggggtcag gcacacctcg
     5041 ccccacaggg catcagacaa gtcggcaatc gcaggccttc gcaaatacgc ttgttccatc
     5101 agttggggct gtgagaagat ctcacgcgga gttccttcac cgatcacacg tccattcgcc
     5161 atgaccacaa ctcgcctggc atacagactg actatgttca tgtcgtgggt gattatcagt
     5221 atggtcttgc ccttctcctt acggagcttc tccaggtagt caagtatctg aagagactcc
     5281 agccagtcaa ggccagtagt cggctcatcg acgatgagta tacgaggttc catagccacg
     5341 acagacgcca gggcaagtct ctgcctttct cccttggtga ggtaacgcgg ccatacttgc
     5401 ctgtacttgt cgagacctat gtcgagaaga atgtcgcgca ctgcctgctc cattctgtcc
     5461 ttatccatgc cgatatttga cggcccaaat ctcagttcat cctcgacggt ggtacaaaaa
     5521 agctgctcgt ccgggttctg gaatacgtag ccaaccatag gacatagctt cgttgtcgat
     5581 tcattcaaga ttgactcgcc gaaaaggcgt atgtctcctg atgaaggcct aagcagtccg
     5641 ttcagatgcc tcgttagagt ggtcttcccc gctccattct gcccgacgat ggccacgaat
     5701 tctcccgaat ccaccttcag gtttacgcca tttaacgcct tagctcccat cggatacttg
     5761 aactcaagat cgatcacttc cactgcaggc gacacaccat caaatgacgt cgtcaacgcg
     5821 ccatgctgca ttttatttcc actgctccca gagatggact gatcatctgc caggccagtc
     5881 tccacacttg cagccggttg ccaggctccg cgctctacca cagagatggc ttcttccact
     5941 gaaaaaggcg cctcggcaga tcctatcaag tctccaaact cgactatgtg aggtaccctc
     6001 acgccagctg acttgagatc gttaacactg ccgaagacct cccgcggcgt acccccaaaa
     6061 acaaacttgc ccgacttcat gacgtacacc cggtccgcca gctgaagtat gtcatctacc
     6121 tcttgctcta ttagaatgat ggtcatcccg agcgacttgt tgaggtaccg cactatcgag
     6181 aagacgcgag ccttcccctc cggatccagc atggatgtgg gttcgtcgag caccattatc
     6241 ttgggcaaca ttgcatagac tcccgcaatc gctgtcgcct gcttctgccc gccggagaga
     6301 cgatgcgggg ccctttgctc caaacccttg agcctcatat cctcaatggc tctgtttact
     6361 cggcccatga tcgtgtcata atcgaaccca aggttagccg gcccaaacgc cacgtcttct
     6421 tctactgtca ttccaaaaag ctgcgtatcc ggatctccaa aaaccaatcc taccttggac
     6481 gccatctgcg cagtgcctac ctcgttaggc acctggccgt caacgacgac ttccccggta
     6541 agctccccct cgagaaaccg cgggactgtt ccgttgatga cacacgcaag agtgctcttg
     6601 cccgcaccgg taggccccag tattgctacg cactcgccct ctttgacatc aagcgaaaca
     6661 ccttttatag cttgtctttt cgatcctttg taggcaaaag tcacgtcctt aagcgagaca
     6721 atggtgggcg ccaactgcaa cacctcttac atcttgaaac tttcatcctc aggctctacg
     6781 agctcttcaa catcgctctt ttcgtgcgtc tgagcactct tcagcgcctg ggctacggtc
     6841 aaaccaacgg ccgcattcac ggccgatccg atcagctgag acggaagaag agcaaccatc
     6901 agagctgggc caatcatgac tacccatggg cctagacagg tgatcgtgtt taccactgtg
     6961 ccgacgatta tcgccaacac aatattggtt tttttggcta cgaaaactcc gatcgtgacc
     7021 gtgaaagcca tcgcacacac gtatatcggg accatcggca accagctggc aaagcccccc
     7081 agggcatttg ctaggaaaca ccctatggca gcgacagtgg cacctatagg ccatccaaaa
     7141 tacgaagctg caaaatagcc agcagcagca tcaagagcaa tcgtggtagc aggtccaggt
     7201 atcttgatca tcgctcctgc ccctgatact gcggccagta ttgtgactcg agagattgtg
     7261 cgcgtatccc aaaccacctt gtgttcctcc ggatattgct tggtcagtat gtctccggct
     7321 agcttcatca gaagcaagta gcaaattgcc aatgcgtatg agaccccgag acccacagct
     7381 ccttgtccaa gcggcatttg gattgcctcc ttaaatagat ttatctgaac ccacgctccc
     7441 gcgcgaggaa ccacccgtca taggccagcc gttgttgtca cgccgccaac ttgaatctgc
     7501 cacgatccaa ttgagtaatt acgatatttc aagcaccctt cgggccgtta ggtcgtcagt
     7561 aatcaacaca tccactactc cggtccttag cgccccaagt atggatgcag ctttgttgac
     7621 accacccgcg acggccacca cgcagtctat ggctcgcagc tgatccagac tggctccaat
     7681 gatgcgatcc cctaagggag tatctacagg ctgcccctgt tcatcaaaga acctcatgca
     7741 tatgtctcca actacgccca tcctaacaag atcttctaat tcatcaggag gaaagccgcc
     7801 ttcttcgaga agggtggaag gaggcacaaa gcaacctact cctaccactg ccacgtctgc
     7861 cgcggcccac ttgctagcga cgttccttat gttgctgtca ttcatcatca tgtttcgggc
     7921 ctcaggtgta tcgacaaatg aaggtgcatg gagatatagg tgggatccgc caaatgccat
     7981 ggctacgcgg gaagcaatat cgtttacttg aagatcgggg ctggcctgac cctgtccgcc
     8041 aaggagagga atcgccatta tctcccttgg ttccggcttg atactgccta atgctgctac
     8101 gagttcacgt aacgttgcgc cccatgaaac cccaatcacc tgaccgggcc ttaactccct
     8161 gtccaaatag ttcgcgcctg ccatccccaa gttctgcgtc aacagccttg aggggccagc
     8221 cttgcctgag atgaccaccg cgtcacttaa gccaaaggta gaccgtagtt tttcttccaa
     8281 atcggcaaag tcgagtcctg gaggaatgag cttgacgacg actatcccct catctttcgc
     8341 ctgtttcaag gccctggaca cttgacttcg cgatatgcct aagctggagg caatctcact
     8401 ctgtgtctta tcgagttcat agtagttctt agcaatttcg tacaacagac gcttgtcaaa
     8461 agccattgta atacccccaa gggtcttttt aggctaggga acgctctcat cagacactct
     8521 tttgcacatt tgttcagcac gtttgtgctg atgggactaa cattctacga agaactccat
     8581 attcctgctt ggagaatgat tttttatgcc aggtctcctc ttctgtccca aaagttacgt
     8641 gagtctcgat gagttcccgc gcgattttcg agtatgcatt tggaagggcg acttgggcct
     8701 gccttcaagg aatatgtcta ttgtttagtc gcttttcggg agcgggtgtc taacttttag
     8761 actggccccg tctaaatttt agccacttga agctctaagg caatcttggg cagggataat
     8821 ccggcatgcc ttgccataag gacccagttt ccggagataa attactatgt ggaagtgcat
     8881 gtacccatat attggattgg ggcgcagaca ggtgtctgag ttttagaccg ctaccgtcta
     8941 ttctttgaac gctttgcgat cgcaaatggc tagaatttag acaaacctgc tcgcccgcac
     9001 ggcacttccc tgggcttcta ccgaaggcta aacagacccc ggctaagtct gtctacctaa
     9061 ctacgaggcc gtcctaaccg ggttgtcttc ccgctcatcg ggcaggcagc tcaacagaca
     9121 tattgggcaa acacgcaaac atccccggag ccgctgcccc ggggatgttc tatcaacatc
     9181 tttccgttgt tcaactacag ccaattgcgc tgtctacaac ccgtgtatgc tacgcaacga
     9241 caatgccacg cagcgttatg cccttcactc catggagaac acgttctgcc cacatctcgt
     9301 ctccgattcc tgtggcccat cccaaggccg ctgtggcaag agatgacaca atcgattgtg
     9361 ttatagcatc agtgtctacg ttgtcgatac ggcccataga tttacccctt gctactacgt
     9421 cttctatgat gtcgacgaac ctgtgccaat aaacgaccac gtcacttgcc cactctcgcc
     9481 ccggggccaa tgcttcctta aacagcaggt acatgaagtc gtcgaaagtc tgggcatagt
     9541 ctatcagcgc gtcaaatatg gcgctgagct gctccacagg atcagtgctg cgcaaggttg
     9601 ctccctcaac gtcctctatt aaggcatcga gtccgttttc gatgatagcg gaaaacagat
     9661 tgctcttgct cttgaaatgg tagtagacag tgcccttagc cacccctgcc tccaaggcta
     9721 tctgatccat ggatgcagca tcatatccct tttcggcgaa tacacgcact gccgtgcggc
     9781 atatgatgcc ccgtgttccc attccgtagc attgtgtcac aacaacctac cccttgttcg
     9841 agggggcctt caccttcatc agaaagcgaa cctcgccggc ggcacctgcg ggcttccctt
     9901 cgaacgtgtc atattgatcg aggcgcactt tcatagcatc caattgagcc ttagccttca
     9961 gcgcctcagc gagcccttcg tttatgccct tcttcatctc cgacgtgccc tcatttcgta
    10021 tcctgcccaa cccggaagca agctccttgg tgccagcagt cagctcaacg gttccgttgc
    10081 tcaacttgct gatgccatcg acgagagcct cgagaccgga tactgcagta tcgagcccgg
    10141 gcacattctg accgtcgact gtaccgccca cagcaagaag actcaaccca gtcttgagat
    10201 catgcgctcc ttcatcgagt ttgcgcaagt tatcgcatat ctcatctatg ctaggcacct
    10261 cgtggccttg cactgtgcct ccatatagga gggcattgag aacctctacg gcccccatgg
    10321 cctggttgag gtaatcatcc acggaatcca gcgacacctt tgatagctgg ctcgatgcca
    10381 gcgaaatggc gatcttagcc ttgcttccca cggaggccac actctttgcg tcctccgcaa
    10441 gctcatcgat gctcggcacc tgtgagaatc caaccctgcc tcccgatttg gcggtctcaa
    10501 gaagggcgtc ctgcttcttg atggaagact ccagtttctt gtactgcagc gatttcgtta
    10561 tctccggatt cgacttggca agcgcctcaa gcgcagccat agattccgaa ctagccgact
    10621 ttgcctgggt tatcgtctgc gagcaactgg acaacccctt ggataccctt tgcgccttgt
    10681 cattgagttc ggtcgcctgt tccaaaagct taggcgccaa ttccgccgct gtgccaagtt
    10741 ggtccattgg cagcgacgac atcgcctcgg atatcgtatc ggaaagtttg accaggatcg
    10801 gcttggctga ctcgaccaat tctatcagct gtttgggttt ttcgagcatc tccggggtaa
    10861 tagcgtcatt catcgtcttt gcgatcgtcc agtgggcatc tgccagcttg ctgaggtcag
    10921 acaccccatt tccgattgca atcatgccgt ccgctacctg agcctgcccg tcagcaagct
    10981 tcagcgtgcc cgactgagcc tgttcaatgg cggaatcgag ctgatcaact ccggacgcca
    11041 acatcccaag cgcgtcgatc gccgtatcca gcaactcttc gagctgagga attggaggca
    11101 tcgaaggtat gacggtgaca tcaatatcgt tcagctggat attctcgcct tccagaacca
    11161 acgtgcacga ggcatccggg ttgggcatca acgtccagtt gatcttgagg ctcttgcccg
    11221 ccaaaaccgt tgtggcgtca ggcgcctgta taagcgtgta gtctggaaga ggcacatccg
    11281 tcgatacctg cactatcatt ggaacgcaaa catcttcggc aaatgtagta ttcttgccat
    11341 caaccgtata gctcagattc tctttcctcg ctgtcctgtt tacaagatcc acagtcactt
    11401 ccagcctgcc ggaactgccc ggaacagcct gagccggaac gaccttgccg ttaagcttgt
    11461 aggtaacctc taccccgatc ggaagctgtt ttttcgaagt tccggagtag tttatgtccg
    11521 ttatccgagt ggggtcagca ttccacttga gcccgccgga aacaacgtcc ggaacctcag
    11581 cattcctgag gtttacagga ttctcaatat tcccagggtc aaaaacagat accgggccac
    11641 cctgcggaac tctgatccag tccactacgg tagtggactc gggcatgcca tcagacgcca
    11701 ggacgcaaaa gaccgtctca tactcctgta ttcttccata agtccctgaa gtgcccgaag
    11761 tctccgagac tcctcccggc gactgtgcca tgcccacact gcagagcatg aggatcgcac
    11821 aggcgcctat tacagccgtg atcttggcag tcctattctt caacgtaagt cacctcgtaa
    11881 aatgatcatt tgtcgtcgga cgccgcaaca gccgacgtcc ccgcctgtct cacagagtca
    11941 tgcttgggcc atccgataga ggtcttctca aggatcggat tgcacacaac cagcgtggca
    12001 gggagcatga aagatgtaac cgccacgctc accaaagcac ccctcgccag caagagagca
    12061 aggtccttga tggccctcag attggtggat gcgtagactc cgattatggc gcagaacatt
    12121 gccaagccgc tgttgagaac cgcaggcccg cattctgcca ctgtcgaccg catcgcatca
    12181 ggcggcgcat tcttgttcag ctttccctta aatgtattgg taaccaagat cgcatagtcc
    12241 acagtagcgc cgagctgtat cgcctcaatc gccatcctgg agaagaagaa gaccggcgct
    12301 ccggtgaaat acgatattgc ctggttaacc cagattgcca ccttaatagc gagtatcaat
    12361 agaattggca ccgtccatga cttgaaggct atcgccacta tggcaaagat gcccgccatt
    12421 gaaacccagt taaccaaatc aaggtccttg gagctgagat cttcaagatc cttgcctatc
    12481 actgaagtcc cggtaaaata gacctctcca ggcaaatcct tgataagctc cctcgtttgc
    12541 gttatgaact ccggcgttct aggatcgaga attgagcctt cgagagtgat cacggtgttt
    12601 tcatactcgc cgcccacgta ctgcttggat accccttcag gcacgaactc cacgggaatc
    12661 ccggggtcga ccacgtctcc aagacacgaa gcgtctttca ccccgtccat ctctcggatt
    12721 tggcgcgtga gttcttgcag cctccactga ggcacgccac gagttattat gtatgcctcc
    12781 tccacactgc caaggtcgtc tttgaactcc tggagggccg caatggaagg cagatccatc
    12841 gggaacgtgc tctctatatc gtagctcggc tcaatcttgt tgtatccgta caacccaacc
    12901 aacgccagaa ctacggtgag ggcaaatacc ggaaccctct gctcagtgag ccagtctccc
    12961 aaccctttgt agcttggaag aatgaccgga tgattccact ttcccaagat tggattgagg
    13021 gtcaacacca agcttggaag gatggtcaga cagcatataa ccgacaacag cacgccgcgt
    13081 gccattagta tccccatatc tgctccgagg gtaatgcgca tcagcgccaa agatccaaag
    13141 cccacagcag tcgtggttgc gcttgacaat accgcgttgg cagtataggt tatcgcccgc
    13201 accatggctt cttcagagtc atgcttctcg cactcggttt ggaatgtgtg aagtagaaaa
    13261 atacaatagt ccatggtaac accgagttgc atagccgaag ccaccgcatt ggtcacatac
    13321 gacacgtcac gccctagcag gtagttgatc cccatgttgt aagcataggc cacgcctatg
    13381 gttcccatga agaccagcgg catcaagggc gacggcaggg tcaagaacaa gaggatcaat
    13441 atgagcagga ccgcagtccg gaccatgaca ggcgtctgtt caacccccaa gtcctttagc
    13501 tccagaccgc tgacgagcgc acctataacc cattgctcct tgtcaagcag gttcttgatg
    13561 tcattgaccg cacgttcagt tcggggcgaa accgcatcct cctcgaaatt gacaatcagg
    13621 agtgtgatct ggcccctgta gaacttagag acgatatcct cgcccaaata atcatggggt
    13681 atcgtcggat cgactaggtc agaaagccag aagaccgagg aaactccgtc aacatgctcg
    13741 agtttctcct tcaccttcag cactgcccaa tcaggctgat tccgacagac tacgaaagct
    13801 cgctccgcat acttgaattc ctcatcgagg acgttcatcc cctcaacgga attgaggtct
    13861 ttaggaagat acgtgagcaa gtcgtactgt accttcctgt gctgcatccc gtagaccgac
    13921 ggaaccaaca gcacggcagc gatgatcagt atcgccaccc tgttttttac gacaaattca
    13981 gctaagcctt tcactgatga gtccctactt ccatacaacg ccttttttta tactgaccgg
    14041 tcagtacaac gatatcctag tcgcttatgg cattaatgtc aaggggtaat ctgtaatatt
    14101 ctgaatttac aaatacgcat aatgcaggga ttttgtgcac g
//
LOCUS       DAOSYL010000063        13280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3926_length_13280_cov_7.413535, whole genome shotgun sequence.
ACCESSION   DAOSYL010000063 DAOSYL010000000
VERSION     DAOSYL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3926_length_13280_cov_7.413535"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..622
                     /locus_tag="PLU15_09055"
     CDS             338..622
                     /locus_tag="PLU15_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOO31106.1"
                     /translation="MVTISVCVGSSCHIKGAPEVIEKFQKLISDYDLYNLVELKGVFC
                     LERCTEGVTIDIDGNIYSAVNPDAAVDIFRERVLAPLGLTPKTGDDNDGQ"
     gene            759..2498
                     /locus_tag="PLU15_09060"
     CDS             759..2498
                     /locus_tag="PLU15_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOO31107.1"
                     /translation="MSLITTIRANCKDCYKCVRHCPMKAIKVVDGHAEVVDELCIGDG
                     TCVRICPQGAKQVRDSRGAVRDILRSGRKVALSVAPSFVSSFNDVDPGSFATAARGLG
                     FDIVTETAEAAYYVTISTMEALDRCEKPAIATSCPVIVNLVERYYPHLLGHLVPVASP
                     MVVHGRMLKNEMGPGSKVVFAGPCIAKKQEAEKEEAAGAVDAVLTFEELDSLFKEARI
                     VPAAQEPSEWNGRVPGVARLFPIEGGMMATAGIPNGGMGSADICITGIEEAIDFLEHA
                     KAHSDIRLAEILSCEGGCVAGPCVLADADLWSRRRKVVEHTLRGRAGSADEEASPEGK
                     RRAVDPPRPELLSMSFSVREVNLPMPTEDEIREILAQTDKFSPEDELNCGACGYNSCR
                     DKAIAVYRGMAEVEMCIPYMRRRAESMSNLIISSTPNAIVAVDRDLRIVLVNPAFESV
                     FRLSAESCVGQHVSVAMDPEPFRRVFETKELISIEEEHLGGELRTSQIVFYVEVRDLV
                     VAIGTDITAEYDTARAIQKAREETLEKAGAVIDRQMRVAQEIAGLLGETTAETKLLLT
                     QLIKQMQDEELTR"
     gene            2498..3640
                     /locus_tag="PLU15_09065"
     CDS             2498..3640
                     /locus_tag="PLU15_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO31108.1"
                     /translation="MSELHVEAAFTSLPKHGEELCGDRVEIARSGDMVTVVLSDGLGS
                     GVKANILATLTSTIAVSMLRGGASIDDVVETIGHTLPICQVRKVAYSTLTILQIKNDG
                     SAYLVQYDNPDAFYYSDGMIVPIESSDRIVADKVIKEARFQLKAGDRLFLVSDGVIYA
                     GVGGVLNLGWGWPNVADYIKKINDQESRASVLAKWLVNVCDQFYAGQPGDDTTAVAVV
                     IREPRCLTVAVGPPKKPEDDRKMVARLMSSVGTKVVCGGTTGKIVAREIGESLHVDLD
                     TIHGGMPPTASINGIDLVTEGIITLSKTLDRLIQGEPVHGKDGASRLARMLLDADHVE
                     LLVGKAVNPAHQNPDLPISTTLKERVMDDIARILVELGKEVSVSYY"
     gene            3700..4197
                     /locus_tag="PLU15_09070"
     CDS             3700..4197
                     /locus_tag="PLU15_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOO31109.1"
                     /translation="MTAVEAAQRRFERVNEIIEKHGRSQSSLVPILQDVQAEYRYLPE
                     EILTYVATAMGLSVATVYGVATFYAQFSLEPKGKYVVRVCDGTACHVRQSMPVYEAVR
                     SKLELADGKFTTPDLMFTVETVSCLGACGLAPVMTINDEVHGKMTPEAACIIIDTLLE
                     REGQI"
     gene            4200..6035
                     /gene="nuoF"
                     /locus_tag="PLU15_09075"
     CDS             4200..6035
                     /gene="nuoF"
                     /locus_tag="PLU15_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOO31110.1"
                     /translation="MAITSREELNACADQFRKELASKKKRVLVCAGTGCLANGSAKVL
                     QEFERLSVDAVGVTKSGCHGFCEAGPLVRIEPDHILYVHVKPEDVEEIVEKTLKLGEP
                     VERLLYHHPVTGKAYLGEGDIPFYKSQTRVALANCGAIDPDDIREYIALGGYKALARA
                     VVDLTPEEIIEEILESGLRGRGGGGFPTGRKWMFAQTAEGSEKYIVCNGDEGDPGAFM
                     DRSVMEGDPHRVIEGLAIAARAIGASHGYVYVRAEYPLAVARLQNAVKQARELGLLGE
                     GIFGTEFAFDVTIKEGAGAFVCGEETALLASIEGKRGMPRPRPPFPAHEGLYGKPTVI
                     NNVETLATVPPIMLHGPSWFRSIGGETSPGTKTFAITGQVANTGLIEVPMGATLREVV
                     FDIGGGVRTGRAFKAAQIGGPSGGCLPAELLDTPLEYDALAKVGAMVGSGGLVIMDEG
                     TCMVEVARFFMKFTQHESCGKCVPCREGTKRMLEILEKIVAGRATMEDLDNLEEIAWV
                     VKESSLCGLGKTAPNPVLTTLRYFRDEYVAHIVDKECPAGVCVAFKHYSIDPELCKGC
                     GKCKRSCPAGAISGEVKNPHVIDVDKCIKCGACAGNCPFGAISLR"
     gene            6056..8098
                     /locus_tag="PLU15_09080"
     CDS             6056..8098
                     /locus_tag="PLU15_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOO31111.1"
                     /translation="MSAQQMVTIDGLAVPIEGERNLLELIRKAGIDLPTFCYHSELSV
                     YGACRMCVVEIENRGVVASCTVPPQPGMVVTTNTEKLRTIRRTIIELLLANHDRDCTT
                     CSKNGACMLQDLARRFGVTNVRFGQADERRRLDRSTPAIVRDPNKCILCGDCVRVCGE
                     VQGIGVLDFAYRGSYSMVTPAFGKELADVDCVQCGQCASVCPTAAITIHSSTDEVWKA
                     IHDKNKTVVAQVAPAVRVALGEEFGHAPGWAEPGKVVAALKKIGFDLVFDTSFTADLT
                     VVEETAEFLERISSGNEGRLPQFTSCCPAWVKYVEQYHPEYLSNLSSCRSPQQMFGAL
                     ARTYLPAQLRVEPEEIYVVSVMPCTAKKFEATRNEFVSDGRPDVDAVITTQELARMIR
                     EAGVRFDDLPVEPFDLPMGMATGAGMIFGNTGGVAEAVLRAASDILEPNKAVGAGSNR
                     RIVFDEVRGVDGVREASLTLGGREVRLAVAHSLGAAAQVLERIKSGEAEYDLVEIMAC
                     PGGCIGGAGQPNPSHLEVRKKRSKALYDVDTMQMLRRAHDNPFVKQLYDKFLGEPCGE
                     TSHELLHTDYGERRRIKGETIKLSSEEVAGQIDVGVCVGTCCYLNGSYDLLKGLMEKV
                     NKAGIEDKVNLHATFCFENCEHSPNIQIDGGIISGVSEDDIERIFREEIMSRLEEQ"
     gene            8114..8758
                     /locus_tag="PLU15_09085"
     CDS             8114..8758
                     /locus_tag="PLU15_09085"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOO31112.1"
                     /translation="MSDSCILMSRATLARLPFYYRKVLAHAQSGHDRISSAQLAEGLS
                     IDSAQVRRDISSIGDFGRAGIGYNVPELIEALEEILGIKNRTEAVLVGVGRLGSALFR
                     YPGFKDYGLKIVALFDSDPEKVGTELDGTLVRHVSEIPHVTERLSIQMGIITVPGKSA
                     QDVADLLCDAGVIAIWNFAPVGLKTPPHVMVRDEDLAAGLAYLSRHIVVSTSSE"
     gene            8953..10296
                     /locus_tag="PLU15_09090"
     CDS             8953..10296
                     /locus_tag="PLU15_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOO31113.1"
                     /translation="MNGIHIFHRNRLGASCPKQYVLSAVIILAAIIVASHSACATDAA
                     SPGKTVFVRVGIPCNGQVTLGSGTAGELLQIGEDLKLDHAQFCVGEDGEIACIGPDGA
                     RKDLHAPILVHLAKEVEEAGGQEAALIWLDNAKAVYKGSFELSVDSKGGLSLVNIVAL
                     EDYVAGVVPYEIGASSPYEALRAQAVVARTEALGGLGRHSSQGFDLCATTHCQVYKGA
                     GAELANPMVRQAVDSTSGEVIFYGDVPAKAANYHACCGGFTESPGDLWGTDVPYMHTV
                     RCNGEIDACGDGVYMSEEDVRNWIETPDPKDYCYGSNGYRWSVVYSQEALAEILQPVI
                     GRGVCVESLEVAARTPRGAAVRLVIHTSSGDFEVAGEYAIRLALGGTQSVKSGVFVVN
                     AYGDAPAKFELVGAGYGHGVGMCQYGARAMAKQGYDYKAILEKYYPGATVGAFRP"
     gene            10432..11004
                     /locus_tag="PLU15_09095"
     CDS             10432..11004
                     /locus_tag="PLU15_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO31114.1"
                     /translation="MNDLRERVRAGDRNALEQLFAENIETVSKMASSIAGARCDIEDM
                     VQEALLRAVRSIRNFRWECSFSTWLYRILVNVNKDFARKACSAEIVSLDQLAEQNDDA
                     LPCVRSEIGQPEEEAIRQEECETLAEAIRALPRLDSSVLWMREALNLSYAEIADNLGC
                     SVESVRSRLWRARRRLRENMALLIPEHVPQ"
     gene            11055..11894
                     /locus_tag="PLU15_09100"
     CDS             11055..11894
                     /locus_tag="PLU15_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO31115.1"
                     /translation="MARVGIVTDSSNGIPPEVIDEYGITVVPIQVQFGAESFKEGVDL
                     PCDKFYRLIEEPVLPTTSQPAPGDFVNAYRSLVGQCDEILSIHLSSKASGTCQSARLA
                     AEMIEGVKITIVDTQTASMGTGLLTIAAAEAAKEGKSSDEIIDLMHRGADGTHVFVAL
                     PTLKYLRRSGRVPQVQAWVASLLSINPILTTHDGVVEAVERVRTFRGSVRRMIELAVE
                     ACGGRAKRVVLMHANAIEDAQGLLDEVQSRISFERIYFSEMGGSMAVHGGAGMLGIAF
                     SQV"
     gene            12019..12093
                     /locus_tag="PLU15_09105"
     tRNA            12019..12093
                     /locus_tag="PLU15_09105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12052..12054,aa:Arg,seq:ccg)
     gene            12371..13204
                     /gene="spo0A"
                     /locus_tag="PLU15_09110"
     CDS             12371..13204
                     /gene="spo0A"
                     /locus_tag="PLU15_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOO31116.1"
                     /translation="MIGRKTRVLVVENNKDLCEVIEAMLKEEDDIELAGTAYDGIQAL
                     EMIPKTQPDVILLDIIMPYLDGLEVLARLDETGVEERPKIVMLSAFEEEEMVRRCARL
                     GADYYLLKPFDRDNLINRIRQVVSAEPLAPKPKTDRAYASRERAREVDRKPRGAQFDL
                     ETETTRILYNLGVPTYFKGYSYIKEAIMLVTRDIGLLGAVTKSLYPKVAEKHGTTPLI
                     VEAAIRYTIQKTWKQGNPEYINKIFGYAVNLREGKPPTNSFFIAKIAEELRLEMLECA
                     Q"
BASE COUNT         3064 a         3086 c         4151 g         2979 t
ORIGIN      
        1 taaagcctga ggccttcggt ttgaccgctc agttagcgcg cgggagtctg atatggaggt
       61 ttggggaccg ggatcgcttt ccaccttgca agcgccgatc ctaggatcat gcaggaactt
      121 attcgcaggg cagttggtct gctcttttgg acgttcttag gcgagctttc acacaactgg
      181 ccccacatgc acgaatgcat caatctcagg tggatgcggg ccgaaagcgg ccaaaatcag
      241 ctgggcgcaa attgtcttgt acgaagtgtt gtatattgtg gcggaaactg ttattattga
      301 attgtgaaag actgcacaaa gttgggacgg cgataaaatg gtcaccatct cggtatgcgt
      361 tggaagctcg tgtcatatca agggtgctcc tgaagtcatc gagaagttcc agaagctcat
      421 atccgactac gatctttaca acctggtcga gctcaaaggc gtgttttgcc tggaacgttg
      481 caccgaaggc gtaaccatcg atatcgatgg caatatctat tccgcggtga atccagatgc
      541 tgccgtggac atcttcagag agcgcgtgct tgcccccctt ggtctgaccc caaaaactgg
      601 agatgataat gacggtcaat gaggcctggt gagccagggc aatgcgtctt ggtgccgggc
      661 cttagctgca cttggagatt gaaaagggct caagggggca aaaacctgaa gaatcgcgag
      721 tgcgactcgg atgtagatga tcaagggggc tgcaacaatt gagtctgatc acgactataa
      781 gggccaattg caaggattgt tataagtgcg tgcgtcactg tcctatgaag gccataaagg
      841 tggtggacgg acacgctgaa gtcgttgatg agctgtgtat aggcgacgga acatgtgtgc
      901 gaatatgccc tcagggagcc aagcaagtcc gggattcccg cggggctgtc cgcgatatcc
      961 tcaggtcggg caggaaggtt gctctttccg tggcgccatc tttcgtgtcg agcttcaacg
     1021 atgtggaccc tggatcattt gccaccgcag ccagaggact tgggttcgac atagtcacgg
     1081 aaacggctga agcggcctac tatgtcacga tatccaccat ggaggctttg gacaggtgcg
     1141 aaaaacctgc catagcaacc agttgtccgg tcattgtaaa cctggtcgaa cggtattatc
     1201 ctcatctatt gggccatttg gtccctgtgg cttcacccat ggttgtccat ggaagaatgc
     1261 tcaagaatga gatggggccg ggatccaaag tcgtatttgc cgggccttgc atagctaaga
     1321 agcaggaggc ggagaaggaa gaggccgcag gcgctgtcga cgcagttttg accttcgagg
     1381 aactggacag tctgtttaaa gaggctcgca tcgtccccgc agctcaggag ccaagcgagt
     1441 ggaacgggcg cgtgccgggc gtggcgaggc tgttcccaat tgagggcggc atgatggcaa
     1501 cggcgggaat cccaaacggt ggcatgggaa gcgcagacat ctgcataaca gggatcgagg
     1561 aggctatcga ttttctggag catgctaagg cccacagcga tatcaggctc gcggagatac
     1621 tttcctgcga aggagggtgc gtagccggac cttgcgttct ggctgatgcg gacttgtggt
     1681 ctaggcggcg taaggtagtg gaacacactc tgcgggggcg tgcaggcagc gctgatgagg
     1741 aggcatcgcc cgaaggcaag cgaagggctg ttgatccgcc aaggccagaa ttgctcagca
     1801 tgtcttttag cgtgcgcgaa gtcaatctgc ccatgccgac tgaggacgag atccgggaaa
     1861 tactcgctca gacagacaag ttctctcccg aagacgagct caactgcgga gcatgcggtt
     1921 acaactcatg tcgcgacaag gctatagcgg tctatcgagg catggctgaa gttgagatgt
     1981 gcattcccta catgcgccgg cgcgcggagt ctatgtctaa cctgataatc agctctactc
     2041 caaatgcaat agtcgctgtg gatcgggacc tgcggatagt actggtcaat ccggcgtttg
     2101 aatccgtttt ccgtctcagt gcggagtcat gtgtgggaca gcacgtcagt gtagccatgg
     2161 atccggagcc tttccgcagg gttttcgaga ccaaggaatt gataagcatc gaggaggagc
     2221 atttaggggg cgagctgcga acatcgcaga tcgtattcta cgttgaagtg cgcgacctgg
     2281 ttgtggcgat cggaaccgac ataactgctg aatacgatac ggcgcgggcg atacagaaag
     2341 ctcgagagga gacgttggag aaggctggag cggttatcga caggcagatg cgagttgcgc
     2401 aggagattgc ggggcttctg ggggagacga ccgctgagac taaattgttg cttacgcaat
     2461 tgatcaagca gatgcaggat gaggagttga cgcgctaatg agtgaacttc atgttgaggc
     2521 tgcgtttact tctctgccca agcatgggga ggagctgtgc ggcgacagag tggagattgc
     2581 ccgcagtggc gacatggtca ccgtcgtgct cagcgatggc ctcggatcgg gcgtaaaagc
     2641 caacatcctg gcgactctca catctactat agcggtgagc atgcttcgcg gcggcgcgtc
     2701 cattgatgat gttgtagaaa ctataggcca cacattgccc atatgccagg tgcgtaaggt
     2761 ggcttactca acactgacga tattgcagat aaagaatgac ggcagtgctt atcttgtgca
     2821 gtacgacaat ccagacgctt tctactattc agatggaatg attgttccaa tagagtcgtc
     2881 tgacagaata gtcgctgaca aagtgataaa agaggctcga tttcagctaa aggctggcga
     2941 caggttattc ctcgtctcgg acggggtgat ctatgccgga gtaggggggg tcctcaacct
     3001 cggttggggc tggcccaatg ttgcagacta catcaagaaa atcaacgatc aggagagcag
     3061 agcatcagtg cttgcaaaat ggcttgtgaa cgtgtgtgat cagttttatg ccgggcagcc
     3121 tggagatgac accactgcgg ttgctgtagt cattcgtgaa ccgcgctgtc tcacggtggc
     3181 ggtcggtccc ccgaagaagc ccgaggatga ccggaaaatg gtggccagat tgatgagttc
     3241 ggttgggacc aaggtagtgt gcggagggac tacaggcaag attgttgcga gggagatcgg
     3301 ggaatccctc cacgtcgatc ttgacactat ccatggaggc atgcctccga ctgcgagcat
     3361 caatgggatc gatttggtta cagaggggat aataacgctt tcaaagacgt tggatcgcct
     3421 tattcagggt gaacctgttc atggtaagga tggcgctagc aggctggcgc gaatgttgct
     3481 cgatgccgac catgtggaat tgctggtggg taaggctgtc aaccctgctc atcaaaaccc
     3541 cgatttgccg ataagcacta cactaaagga acgggtgatg gatgatatag cgaggattct
     3601 tgttgagctt ggcaaggagg tttcggtttc ttactattga gcgccgattg ctgggcgctg
     3661 aagcggaaca gaaaaaaatc tttgtctgga ggcaggagaa tgacagccgt tgaggcagcg
     3721 cagagaagat ttgaacgtgt aaatgagata atcgagaagc acggtcgcag ccagtcttcg
     3781 cttgttccaa tactccagga tgtgcaggcg gaatacaggt atctccctga ggagattctt
     3841 acgtacgtcg ctacagcgat gggtctgtct gtcgcaacag tgtacggtgt agccaccttc
     3901 tatgcccagt tttccttgga gcccaagggc aagtacgtgg tgagggtgtg cgacggcacg
     3961 gcgtgccacg tcagacagtc aatgcctgtg tacgaggctg ttagatcaaa gcttgagctt
     4021 gctgacggaa aattcacaac tcccgacctg atgtttaccg tagaaaccgt atcgtgcttg
     4081 ggcgcatgtg ggttggcgcc tgttatgacg atcaatgatg aagtccacgg gaagatgact
     4141 ccagaggcag catgcatcat aatagacact ctgctagaaa gggagggcca gatctagaaa
     4201 tggcgattac gtcgagggaa gaactgaacg cctgtgctga ccagttccgc aaggaacttg
     4261 cgtccaagaa gaagcgagta cttgtctgcg cgggcacggg gtgtctggcc aacggctccg
     4321 ccaaggtttt gcaggagttt gaaaggcttt cggttgatgc cgtaggtgtc acaaaaagcg
     4381 ggtgtcatgg cttttgtgaa gcggggcctt tggtgaggat cgagcccgac cacatactgt
     4441 atgtacacgt gaagcctgag gatgtagagg aaatagtcga gaagactcta aagctggggg
     4501 agccggtgga gcgtctgctc tatcaccatc cggttaccgg gaaagcgtat ctaggcgagg
     4561 gcgacatacc tttttacaag agccaaacca gggttgcttt ggcaaattgc ggggccatcg
     4621 accccgatga cataagagaa tacatcgctc ttggcgggta caaggccctt gccagggctg
     4681 tggtagatct tacgcctgag gagatcatcg aagagatcct cgaaagcggg ctcagaggcc
     4741 gtgggggagg agggttcccc actgggcgga aatggatgtt tgcgcagacc gccgagggca
     4801 gcgagaagta tatagtctgc aatggcgacg agggagatcc cggcgctttc atggaccgaa
     4861 gcgtcatgga aggtgatcct caccgagtta tagagggctt ggccattgct gcaagggcga
     4921 ttggcgcatc ccacggctac gtctatgtca gggctgaata tccgttggct gtggcgcgct
     4981 tgcagaacgc ggtgaaacaa gctagagaac tgggcttgct aggagagggg atatttggga
     5041 ccgaatttgc cttcgatgtg actatcaagg agggcgccgg cgcttttgtg tgcggagaag
     5101 agacggctct cctagcatcc atcgagggga aacgaggcat gccccgaccc cgtccgccct
     5161 ttcctgcgca cgagggcctg tatggtaagc ctactgtcat aaataacgtt gagactttgg
     5221 caactgtccc tcctatcatg ctccatgggc cttcatggtt ccgcagcatc gggggggaga
     5281 cgagtccggg gacgaagaca tttgccataa cgggccaggt ggccaatacc ggactgatcg
     5341 aggttccgat gggtgcgact ttgagggaag tcgtcttcga catcggcggt ggcgtgcgga
     5401 ctggccgggc attcaaggct gcgcagatag gcgggccatc aggagggtgc ctgccggctg
     5461 agctacttga tactccactc gagtacgacg ctctggcaaa ggtcggcgcg atggtaggtt
     5521 ccggcggcct ggtgatcatg gatgaaggaa cctgtatggt tgaggtggca cgctttttca
     5581 tgaagttcac gcaacacgaa tcctgcggga agtgtgtgcc gtgtcgtgag ggaaccaaga
     5641 ggatgcttga gatcctcgag aaaatcgtgg ccgggcgtgc aaccatggag gaccttgata
     5701 acttggagga gatcgcgtgg gtcgtcaagg agagttcctt gtgcggcctg ggcaagactg
     5761 ctccgaatcc ggtccttacc acgttgcggt atttccgcga tgaatacgtt gctcatatag
     5821 tggacaaaga atgtccagcc ggcgtgtgcg tggcctttaa gcattattcc atcgacccgg
     5881 agctgtgcaa aggctgcggc aagtgcaaga ggtcgtgccc tgccggggcc atcagcggcg
     5941 aggtgaagaa tccccacgtt atagatgtgg ataagtgtat caagtgtgga gcgtgcgctg
     6001 gaaattgtcc gttcggtgct atctcactgc gatgaccggg ggagggcgga aatcaatgtc
     6061 tgcacaacag atggtaacaa tagacggtct ggccgttcct atagagggcg agcgcaatct
     6121 gctggagctc atacgcaagg cgggaataga tcttcctaca ttctgctacc actccgagct
     6181 ctccgtttac ggagcatgca ggatgtgtgt ggtggagatc gagaacagag gggtagtggc
     6241 gtcctgcacg gtgccgcctc agcccggcat ggtggtgacc accaacaccg agaagctgag
     6301 gacaataagg aggacgataa tagagcttct cctggctaac catgaccgtg attgcactac
     6361 gtgtagcaag aacggggcgt gcatgcttca ggacctggcc aggcgtttcg gcgtgacgaa
     6421 cgttaggttt ggacaagcag acgagaggcg tcgcctcgac aggtctacgc cggctatagt
     6481 gcgcgacccg aataagtgca ttctctgtgg agactgcgtg cgtgtatgcg gcgaggtgca
     6541 aggcataggg gttttggact tcgcataccg cggttcatat tccatggtga cgccagcttt
     6601 cggaaaagag ctggctgatg tagactgtgt ccagtgtggg cagtgcgcca gtgtatgtcc
     6661 gaccgccgca ataactatcc actcgtccac agatgaggtg tggaaagcaa tacatgacaa
     6721 gaataagacg gtggtggctc aggttgcccc ggctgtgagg gtagcccttg gagaagagtt
     6781 tgggcatgcg ccaggctggg ctgaacctgg caaggttgtg gctgccttga agaagatcgg
     6841 gttcgatctc gtcttcgaca cttcattcac tgccgacctt acggttgtgg aggagaccgc
     6901 ggagttcttg gagaggatct catcgggcaa cgaggggcgt ttgccgcagt ttacttcatg
     6961 ttgccctgca tgggtgaaat atgtcgagca gtatcacccg gagtatcttt cgaacctatc
     7021 gtcatgcagg tcgccacagc agatgttcgg cgccttggcc cggacatatc tcccggccca
     7081 actgcgtgtg gagcccgagg agatatacgt ggtttcggtg atgccgtgca cggcgaagaa
     7141 gtttgaggcg acgaggaatg agtttgtttc agacggcagg cctgatgtgg acgctgtcat
     7201 caccacacag gaacttgccc gcatgatacg ggaggccggg gtcaggttcg atgatcttcc
     7261 tgttgagccg tttgaccttc ccatggggat ggctacaggc gcagggatga ttttcgggaa
     7321 tacaggcggg gttgccgagg ccgtgctccg agccgcttct gatatactag agcccaacaa
     7381 ggctgttggg gcgggcagca acaggagaat cgtatttgat gaggtgcgag gagtcgacgg
     7441 tgtccgggag gctagcttga ctctgggcgg acgcgaggta aggcttgcag tcgcccactc
     7501 gctgggagcg gcggcgcagg tgcttgaaag gatcaagtcg ggcgaggccg agtatgacct
     7561 tgttgagatc atggcgtgtc ccggagggtg catcggaggc gccggacaac ccaacccgtc
     7621 ccatttggaa gttaggaaga aaaggtcaaa ggccctatat gacgtggata ccatgcagat
     7681 gttgcggaga gcgcacgaca atccgttcgt gaagcagcta tacgataaat tcctgggtga
     7741 accgtgtggc gagacgtcgc atgagttgct ccacaccgat tacggcgagc gcagacgcat
     7801 aaagggagag actatcaagc tctcctcaga ggaagtggcc ggtcagatcg atgtaggcgt
     7861 gtgcgtaggc acgtgctgct atctgaatgg atcgtacgat cttctcaagg gcttgatgga
     7921 aaaggttaat aaggcgggga tagaagacaa ggtaaacctt catgccacgt tttgtttcga
     7981 gaattgcgag catagtccta atatccagat tgacggaggc ataatatccg gagttagcga
     8041 agacgacatc gagaggatat ttagagagga aatcatgtcc cgcctcgagg agcaataggc
     8101 gaggggtggt ttagtgtctg attcatgtat tctaatgtca agggcgactt tggcgagatt
     8161 gccgttctat tatcggaaag tgttggcgca tgctcaatct gggcacgata ggatctcctc
     8221 tgcccagctt gctgaaggcc ttagcatcga ctctgctcag gttcgtcgtg acatctcttc
     8281 aattggcgat tttggaagag cgggcattgg ctacaatgtc cctgagctta tcgaggcgct
     8341 cgaggagata ctagggatca agaatcggac tgaagcggtc ttggttggtg tcggacgtct
     8401 cgggtctgca cttttcagat accctggctt caaggactat gggctcaaga tcgtggcctt
     8461 gttcgattcg gatcccgaga aagtagggac ggagctagat ggcacgttgg tgcgtcatgt
     8521 gtcagagata ccgcatgtga ccgagaggtt gtcgatccag atggggataa ttactgtgcc
     8581 tggaaagagt gcccaggatg tggccgatct cctgtgtgac gcaggtgtga tagctatctg
     8641 gaactttgcg cctgtggggc tcaagactcc gccgcatgtg atggtcaggg atgaagattt
     8701 agcagctggg ttggcatatc tgtctaggca catagtcgtc tctacttcca gcgaatagtc
     8761 aaagaaggaa atacaagcga gtttgtggaa acattccggg gcaatcgtgc acacggcgcg
     8821 gttgccccgg aaacgtttct tctgtgattg ttggcaggcc caattccctt ggtcacgcgc
     8881 gagcggtcgt gtctgcatgc tttaagattg cggtcatcgg ctctcaaagg ccatgatgga
     8941 ggttaacccg atttgaacgg cattcacatc tttcacagaa accgactcgg ggctagctgc
     9001 ccaaagcagt acgtcctatc ggcagtcatc attcttgcag ccatcattgt agcatcgcat
     9061 tcggcatgtg caacggatgc ggcatctccc ggcaaaactg tgtttgtgcg agtgggtata
     9121 ccctgcaatg gccaggtaac actgggctct ggaacggcag gagagctcct gcagataggg
     9181 gaagacctca agcttgacca tgcccaattc tgcgtgggcg aggatggaga gatagcatgc
     9241 attgggcctg atggggcccg caaggacctg catgctccga tcctggtcca cttggcaaaa
     9301 gaggtggagg aggctggcgg gcaggaggcc gcgctcatat ggctcgacaa tgccaaagcc
     9361 gtctacaagg gatcattcga gctttccgtc gattcgaagg gaggcctgtc cttagtcaac
     9421 attgttgcgc ttgaggatta tgttgctggc gtggtgccat atgaaatcgg ggcgtcttct
     9481 ccatacgagg cgttgcgagc ccaggcggta gtggcgcgaa ctgaggctct aggcggcttg
     9541 ggacgtcatt cgtctcaagg gttcgatctt tgcgcgacga cgcactgcca ggtatacaag
     9601 ggagctggcg cagagcttgc caatcccatg gtcaggcagg cagtggattc cacttcggga
     9661 gaggttatct tttatgggga tgttcccgcg aaagcggcga attatcatgc gtgctgtggg
     9721 ggcttcactg agagcccggg cgatttgtgg ggaacagatg ttccatacat gcatacagtg
     9781 agatgcaatg gagagattga tgcctgtggt gatggcgtgt atatgtcgga ggaggacgtt
     9841 cgtaactgga tcgaaacacc tgatcccaaa gattactgct acgggtcgaa tggctaccgg
     9901 tggagcgttg tctattctca ggaggcgctt gctgagatct tgcagccggt gatcgggcgg
     9961 ggcgtatgtg ttgagtcatt ggaagttgcg gcgaggacgc cgaggggcgc tgctgtgcgg
    10021 ttggtaatcc atacgtcctc aggcgatttc gaggtggctg gcgaatacgc gataaggttg
    10081 gctctaggcg gaacccagtc tgtcaaaagt ggagtgttcg tggtgaacgc atacggggat
    10141 gctcctgcga agttcgagct tgtgggcgcc ggttatgggc acggggtcgg aatgtgccag
    10201 tatggtgcga gggccatggc taagcaggga tatgattaca aagctatcct ggagaaatat
    10261 tatccagggg cgactgttgg agcatttaga ccgtaaaagg acggcctgtg taggttcgag
    10321 ccgattctat gcgtcatgtt ggatgttgcg cagtctgggg tggatgtccg gataatgtcg
    10381 gtcgggagtt ggcgcgggcc ggttccgatc gtttgagggt gatatggggc aatgaacgat
    10441 ttgcgcgagc gcgttcgtgc tggcgacaga aacgccctgg agcagctttt cgccgaaaac
    10501 atcgagacgg tctcgaagat ggcatcgagc atcgccgggg cccgctgcga catagaggac
    10561 atggttcagg aggctctgct ccgagccgtt cgctctatcc ggaactttcg gtgggaatgc
    10621 tctttctcca catggcttta caggatcttg gtcaacgtaa acaaggattt tgcgcgaaag
    10681 gcgtgctctg ctgagattgt ttccctcgat cagctggccg aacagaacga tgatgcgcta
    10741 ccttgtgtgc gctccgagat cggacagcct gaagaggagg ccattcggca agaagagtgc
    10801 gaaactttgg ccgaagcaat ccgggctttg ccgcgccttg acagctctgt tctgtggatg
    10861 agagaggcgc tgaacctgtc atacgcagaa atagctgaca accttgggtg ttctgtcgaa
    10921 agcgtacgtt cgaggctatg gagagctcgg aggcggttgc gggagaacat ggccttgcta
    10981 attccagagc atgtcccgca gtgacacttt actcccgttc ccccgtctaa ctgttttgga
    11041 ggggggaatc atctttggcg agggtcggaa ttgtcacaga cagctccaat ggcataccac
    11101 ctgaggtaat cgatgaatac gggataaccg tagtgccgat ccaggtccag ttcggcgccg
    11161 agtcattcaa ggaaggcgtg gatcttccgt gcgataagtt ttacaggctt attgaagaac
    11221 ctgtgctgcc cactacatcg caacccgcac cgggtgattt tgtcaatgca tacagaagct
    11281 tagtgggaca atgcgacgag attctttcga ttcacctatc atcaaaagcg agcggcacgt
    11341 gtcaatctgc gaggctggcg gctgagatga tcgagggcgt caagataacc atagttgaca
    11401 cacagactgc aagcatgggc acgggccttt tgacaatagc tgcagccgag gctgccaaag
    11461 aaggaaaaag ctcagatgag atcattgacc tcatgcacag gggcgcagat gggacacatg
    11521 tgtttgttgc ccttccaacc ttgaaatact tgcgccgcag cggcagggtt ccgcaggtgc
    11581 aggcttgggt tgcatcactc ctttcgatca accccatact tactacgcat gacggtgtgg
    11641 tcgaagccgt cgaacgagtt cggacattcc gcggttcggt aaggcgaatg atagagcttg
    11701 cagtggaggc ctgtggtggc cgcgcaaaaa gggtcgtgtt gatgcatgct aacgcgatcg
    11761 aggatgccca aggcttgctg gatgaagtgc aatcaaggat ttccttcgag cggatctact
    11821 tctctgaaat gggaggatct atggcagtac acgggggcgc agggatgttg ggaatagctt
    11881 tttcgcaagt ataggggtcc ttaaggcacg cgcggaagtt gtgcgatgcc ttcagctaga
    11941 aatggtgatg ttctcggtgc cggacatcat ttgcgaactt cgccgtttag gcgtataata
    12001 cactcgaaca gttggaacgc gcctatagct cagtggatag agcactggcc tccggagcca
    12061 gttgcgcagg ttcaattcct gctaggcgcg ccatttattt gagtccctct gcagatcggc
    12121 agagggctat tattttcgtg gagtcggcca tatttcacgg cgttcccgga gaagttggtg
    12181 agtagctcta cagttttcga gatattccgt aacattacga aggcaatttc agatggcgtg
    12241 tcgatgaata caacataatc gcgtgtcaat tatgtccaat tgagcaggga cttgcatgcc
    12301 cctcgtagaa tggtccattc gcgttaattt tacgcgcttc aaacaactac ggcaataggg
    12361 gggaagaagc ttgattggga gaaaaactag ggttctagtc gtcgagaaca acaaggacct
    12421 gtgcgaagtc atcgaggcaa tgctgaaaga agaggacgac atagaactgg ccggaacggc
    12481 ctatgacggg atccaggctt tggagatgat acccaagacc caaccggacg ttatcctcct
    12541 cgacatcatc atgccgtatc tcgatggact ggaggttttg gcccgcttgg atgaaacggg
    12601 cgtcgaggaa cgcccaaaga tcgttatgct ttcggcattt gaagaggaag agatggtcag
    12661 gagatgtgct cgtctcggag cggattacta tcttcttaaa ccttttgata gggacaacct
    12721 tatcaaccgg atacggcagg tagtcagcgc tgaaccgctt gccccaaaac ccaagacgga
    12781 tagggcttac gcctctcgag agagggcgcg tgaagttgac cggaagccca gaggcgcgca
    12841 gttcgatctg gaaacagaga ctacaaggat tctttataac ttgggtgtgc ccacctactt
    12901 caaaggctac tcctacataa aagaagcaat aatgctcgtc acacgcgaca tcgggctctt
    12961 gggcgcggtc acgaagtcgc tctatccgaa agttgccgaa aagcacggca ccactcccct
    13021 catagtcgag gccgctataa ggtacacaat acaaaagaca tggaaacagg gaaaccctga
    13081 atacatcaac aagatctttg gctatgccgt gaacctacga gaaggaaaac cgccgaccaa
    13141 ctcgtttttc attgccaaaa tcgcagagga gctccgcctg gaaatgcttg agtgcgcgca
    13201 gtaagtctgc tatgcaatga aatgggcgaa cccgcggcgg gccttcggtt cgccgctgtt
    13261 tgctttgcat acgcagcccg
//
LOCUS       DAOSYL010000064        13098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_3992_length_13098_cov_7.976232, whole genome shotgun sequence.
ACCESSION   DAOSYL010000064 DAOSYL010000000
VERSION     DAOSYL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3992_length_13098_cov_7.976232"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..568)
                     /locus_tag="PLU15_09115"
     CDS             complement(14..568)
                     /locus_tag="PLU15_09115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HOO31117.1"
                     /translation="MNGELEKMLSGELYDAGDPELYRMRLRARRLTREFNATTEEEHD
                     LRATILKELFASVGSAFIEPPFYCDYGSNICVGKNFYMNFNCVILDCAAVEIGDNVMA
                     GPGVHIYTAYHPLDAAVRVQGLELAAPVKIGNNVWIGGGAIICPGVVIGDNTTIGAGS
                     VVTRDVLANVFAAGNPCRVMRDLE"
     gene            1200..1679
                     /locus_tag="PLU15_09120"
     CDS             1200..1679
                     /locus_tag="PLU15_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOO31118.1"
                     /translation="MHSSTYDPTPVTELYGVDPLSLRVFHSFFKAMRLYRQLIFKVLS
                     EKEIYPGQAGCLWVLSGHDGIAQRDLARKLHVASSTVTVMLQKMEAAGTIVRKTDEED
                     QRLSRIYLTDAGRKIQEEMNEALADFVNATFGKMPSGDREEFDRLLSALCSNISQEL"
     gene            1710..3449
                     /locus_tag="PLU15_09125"
     CDS             1710..3449
                     /locus_tag="PLU15_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31119.1"
                     /translation="MTRLLQYLKPYQKQLFLVMALLLVQAIANLYLPDLNADIINNGI
                     ARGDTEYILKTGGFMLLVTLLLAACSVVSVYWGSKTAMSFGRDLRSDVFRKVQSFSQA
                     EVDQFGTPSLITRTTNDVQQVQMAVLITLNVMISAPVMCIGGIIMALRQDVPLSISII
                     VIVPVMAVVIGVIMRRALPLFKSMQVKIDRLNQVVREKLAGVRVIRAFVKTDYEEARF
                     DEANLDLTTTALKINRMVAITMPLLMLIVNISMAAIMWFGAHRIDSGAMPIGNLTAFI
                     AYTMQILIAVMMATVMFIMIPRASASAERILEVLETEPSISAPKAPVALNPGTADSDA
                     SVEFRDVEFTYPGAETPVLRGISFIAYPGETTAIVGGTGCGKSTLINLIPRFYDVTGG
                     SLLIDGVDIREIPEEDLRNKIGFVPQKAFLFSGTIASNLRYGKRDATDDELWHALRIA
                     QAADFVAEMPGQLEAPVSQGGTNFSGGQKQRLAIARALVKRPKIYVFDDSFSALDFKT
                     DAALRAALNDEVDDAIVFIVAQRVSTIMNAERIIVLDKGEIVGMGTHEELMKLCSVYR
                     EIVLSQLSEEEVA"
     gene            3446..5395
                     /locus_tag="PLU15_09130"
     CDS             3446..5395
                     /locus_tag="PLU15_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31120.1"
                     /translation="MSEEKTAARRQRGHGPRGAMMPGEKARDFKGSLKRLFGYLKPHK
                     TQLIIVMVLSIACVVLTIAGPKILGKATNILFEGVIGKQLPPGLGKEQIISMLKAAGK
                     DQMAEMVAGMNIVPGAGVDFEAIKRILLILVGIYIANALFSWLQHYIMAGISQRTVYS
                     LRKEADAKLGRLPLKYYDSNTRGEILSRVTNDIDNIASTLQQSLTQLVTATFTVIGVL
                     IMMFSISPLLAAIALVVLPLGVIVTLFIAKRSQKQFAEQWDSTGALNGHIEEMYTGHR
                     IVKAFNRQQEAIDEFDKENDRLYQASFKAHFISSTIQPAMQFINNLNYVAICVIGGMK
                     VANGVLSLGDVQAFIQYSRQFTQPIVQTASIMNVLQSTVASAERVFELLDEEEETPDT
                     ENPVVLEKVEGHVVFKNVSFRYKPDVPLIEDMNLEVKPGQTVAIVGPTGAGKTTVVNL
                     LMRFYELDGGSISIDGVDIRDMARNDLRRLFGMVLQDTWLFNGTIRDNIAYGREDATE
                     EEILAAARAAHVDHFVRTLPKGYDTELGDEASNISQGQKQLLTIARAFLADPDILILD
                     EATSSVDTRTEVLIQEAMAELMRGRTSFVIAHRLSTIRNADTILVMDNGRIVEQGNHE
                     ELMAARGFYYDLYNSQFAGAYDEAV"
     gene            5647..6501
                     /locus_tag="PLU15_09135"
     CDS             5647..6501
                     /locus_tag="PLU15_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOO31121.1"
                     /translation="MENLGIVTERIHSKYASMTKSQQRLADFVLQNMPEVAMMSSADL
                     ACCMDVSEATVVRFAQMLEFNGYPDMRQQLQTELLKQFRSSSRVAAMVHAQSDNAGPL
                     TMVVSETIKSLQLLLQNVNDAQVEEAVQRIISARRVIVFGEGAPASITVLADFWLSRL
                     GCQVVRVNQTGRRLFDKIFQAGSEDLAIVFAFRRVTLEAMAILEHLNQEGGQSLLVTD
                     LIDSKMHPLATQVFLVQRGPMEAFRPMGAPLALVDALILGVMLAKGDDAVEQLSRLDT
                     LRQRYDCL"
     gene            6526..7746
                     /locus_tag="PLU15_09140"
     CDS             6526..7746
                     /locus_tag="PLU15_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO31122.1"
                     /translation="MSQRIIKLVILCVFVGMIGAYVGAPRAYSAPKLPADVDAWLKQV
                     EIGPYQPAKVDWDKVYQAAKREGKVVIYTSSSRTISLKSEFESLYPGVTMEVYELGTT
                     GAIEKVEREQLAGVYNADILHASGYADQLHLLYNKNMLFPFYPPELESVIPRAFREPL
                     VAQRYESRTIFYNSKVYPKCPINSWWDLTKPEWRGKIAMVDPVNDASSLDLITTIVLN
                     ADDLARDYQRVFGRPIHLTTPNAGYELLKGIIANKPRIYSRHTDLINVVGDPTNKNPP
                     IGISIPFSSLGYAEDPARGSLKLMATTNITPTVGLLYPSLMNITYKAPHPNAAKLLIK
                     YLFGDQYGGLGMKPFFIVGNWPARTDMLAEPKNQFLPNLSLPLKSMKFWMLDPIGIWK
                     TSAEVQEWWMININ"
     gene            7842..9650
                     /locus_tag="PLU15_09145"
     CDS             7842..9650
                     /locus_tag="PLU15_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO31123.1"
                     /translation="MNNVKLHGRKAWNQLRKPESILSILFLIILAYLIIVPLISLICR
                     TFVWGTGDLRIRGENVEPGKFTLYHWRRMLTGDFAKSVFYKPLLNTLTITVGTVAIIL
                     LISTLLAYLVVRTDIRWAKLINALAIVPYILPSWVLSLSWLQIFQNSAVALPDGLLFF
                     LTGKNAPEWLVYGPIPIMITLGLHYYPFGYLLISGALSTVDSQLEESAEILGANQRFI
                     RRHITMPIVSPALLSTALLGIARSMGTFGTPAILGLPARYYTLSTQIYSLIHTQREAQ
                     AYILAIVLLLISSTGLYLNQKLISSRRSYATIGGKGVKSRKVKLGKWNTPIAVITLIF
                     LFMVSVFPLGFLVWSSFCLNAGDYSISNLSSHYWIGASNPRFAAGEPGILRNEAVRGA
                     AWNSVRLSLISALICGLFGMLIGYVVVKSRNSPIAHMLEQISFAPMLVPSIVFGALYL
                     TLFAVRRGPIPALYGTFTLLVLVTIAKQLPYTVRSGVSSMLQVSHELEEAAVILGASW
                     RERMRRIMLPLTKSGFVSGALIVLITSMRELSLYILLVTPANRMLTTLTYRYIEVGYT
                     QFSNAMTVLLTLLVLLMTWAIKAWQNMDIAEGLGGM"
     gene            9653..10753
                     /locus_tag="PLU15_09150"
     CDS             9653..10753
                     /locus_tag="PLU15_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31124.1"
                     /translation="MEAVKLVGVTKEWPGVKAVDNLSFDIGKGDFATLLGPSGCGKTT
                     TLRMIAGLEDPDAGDIYINGKLVFSASQGINLPPAARGLGIIFQSYALWPHMTVFDNV
                     AFGLVQQKMPRDKIEAKVFGTLKQVGLEDLADRYPSELSGGQQQRVALARMVIVEPEL
                     LLMDEPLSNLDTRLRMQMRASLKRMHQEANTTVVYVTHDQEEALTLSTKIAVMNHGQL
                     EQYSPPEDVYERPATHFVAEFTGNPQTNFLDAKVVRNGNSPYLAVGDTVLWPAQPGWP
                     DQVVLAIRPEGLTLCNDNQPGRIPAAVYATLPVGPSVLVYADSNGIEIVIRADRGTSF
                     QGNQQVWLELDRSQINIYDAETGKRIGDDTDH"
     gene            10737..11567
                     /locus_tag="PLU15_09155"
     CDS             10737..11567
                     /locus_tag="PLU15_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOO31125.1"
                     /translation="MTQITDKNNVIRDIKGFMFDLDGVLWLGDHPIPGVANTLKQLRR
                     EGKQLMFVSNTSSRSKAKCLSIFKHMGLDVDSSEVFIASECAAKYVASQMPGAITYVL
                     GSEGLLEEALAAGLDARNASTRNPEKAHYLIAGKDNDLSFARLTCALRVLQSGARFVA
                     VNCDPTVPSQDGLEPGAGAIVAAITAMVNRDPDVIVGKPGTLLLEMALEQSGLDAAEC
                     AIVGDTLETDILAGNRLGLSTILVLSGNSKGLDMSAPDAYPIEFRPDIVMNSVEDLVY
                     "
     assembly_gap    11943..12042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12043..12351)
                     /locus_tag="PLU15_09160"
     CDS             complement(<12043..12351)
                     /locus_tag="PLU15_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31126.1"
                     /translation="MQSIASGISRKIIFISEAVCGPLVDHSLYRIMFAHRKYLVKSIR
                     GIYKQADTFDKADGGCGVNGEAQVQVNRLAITGATRSVRQTSSVDPYLSPVFYCEANF
                     C"
     gene            complement(12737..>13098)
                     /locus_tag="PLU15_09165"
     CDS             complement(12737..>13098)
                     /locus_tag="PLU15_09165"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOO31127.1"
                     /translation="LSFRYKTDDQLWFTFFHEAAHVLGGYQSSAFIDNFEPNDLASEV
                     ELEADRFAADILVPPTEYRKFTEGIQGGRISRNKVSAFAKSIGVAPGIVVGRLQHDGL
                     LPYTHLNGLKVRLKWPT"
BASE COUNT         3305 a         3528 c         3257 g         2908 t
ORIGIN      
        1 ggaaaaatga gcgctactcc aagtctctca tcacccgaca gggattcccc gctgcgaaca
       61 cattggccaa aacgtcccta gtcaccacgc ttcccgctcc gatcgttgtg ttatcgccga
      121 tgacaacccc agggcatatt atggcccctc cgcctatcca gacgttgttt cctattttga
      181 ctggcgccgc aagttcaagg ccttgcaccc tcactgcggc atcaagcggg tggtatgcag
      241 tgtagatatg aaccccggga ccggccatga cgttgtcacc gatctcaact gcagcgcagt
      301 caagaatgac gcagttgaaa ttcatgtaga agttcttgcc gacgcatatg ttacttccat
      361 aatcgcagta gaaaggcggc tcaatgaagg cgctgcccac cgatgcaaac agctccttca
      421 aaatcgtcgc acgcaagtcg tgttcctctt ccgttgtggc gttaaactcc cgggtcaacc
      481 ggcgagctct gagacgcata cggtacaact cagggtcgcc ggcgtcgtaa agctcgcccg
      541 agagcatttt ttccaattcc ccgttcatat cggagccccc tttggcaaag tggacaatat
      601 gccacctgag actaaaaggc agaacatcgg agcatggctc ccgcccctaa agatgccgct
      661 tgtcaactgt cattgtaatg gaagaatctc aactcggcca cgcgaacacg ttggtgcgaa
      721 caccgaagct ttgagctttc aaagtcttat acggacaatc gaatagctcc gctgtgggaa
      781 acaaaaaggc atactacagg cagtagagta tctatccaag ctcatagaag cattcggact
      841 ggacataccg atgctatgca gggcaaatct gacgattcgc agtatagccg gaaaaggtca
      901 ggagggagag cattcgaatt gagcgcagca ggcgttggcg aaacggacct aaacccctct
      961 gttagcagcg gatagagttt gcagcgtgta cggggacgcg aaatcccctc ccacggagcc
     1021 cgcccagacg ctaagagctt catactactg attcccagcc acattcaaca catccctgtt
     1081 gacctcgcag ccaagacgcg ctacaataca ttacgcatat agttagcatg ctacctatta
     1141 gcatggcaac gattatgtgc tcggggcctg gcctacgatc gctgaaaggg ggtctaacag
     1201 tgcattcatc gacatatgat cccacgcccg tcacagagct ttatggagtc gatcctctat
     1261 cgttgcgcgt cttccattcc tttttcaagg caatgcgctt gtatcgccag ctaatcttca
     1321 aagtgctgtc tgaaaaggaa atttaccccg gacaagccgg atgcctctgg gtcctcagcg
     1381 gtcatgacgg cattgcccag cgggacctag cccgaaagct tcatgtggcg tcatccacgg
     1441 taaccgtgat gcttcagaag atggaggctg caggcacgat agtccgaaaa acggatgagg
     1501 aggaccaacg actgtcccgc atatacttga ctgatgcagg acgcaaaata caggaagaga
     1561 tgaacgaggc gcttgcggac ttcgtcaacg ccaccttcgg caaaatgcct tccggcgacc
     1621 gcgaagaatt cgacaggctc ctgagcgccc tgtgttccaa catatcacag gagctttgat
     1681 ttgcttacat gagaggtgtc agtttctgaa tgacaagact acttcaatat ctcaagccct
     1741 accaaaagca gctgttcctc gtaatggcac tccttctggt ccaggctatt gccaatttgt
     1801 acctgcccga cctcaatgcc gatatcatca acaacggcat cgctaggggc gacaccgaat
     1861 acatcctgaa gacaggcggg ttcatgctgc tcgtcacctt actgctggcg gcatgctccg
     1921 tggtctcggt atattgggga tcgaaaacag caatgtcctt cggccgcgac ctcaggagcg
     1981 acgttttccg caaggtgcag agtttttccc aggcagaggt ggaccaattc ggtacaccct
     2041 cactcataac ccgcaccacc aacgacgtgc agcaggtaca gatggcagtc ctcataaccc
     2101 taaacgtcat gatctccgcc cctgtaatgt gcatcggcgg catcatcatg gctctccgcc
     2161 aggacgtgcc attgtccata tccatcatcg tcattgtgcc ggtaatggcc gtggtcatcg
     2221 gtgtgatcat gcgacgcgcc ctgccgctat tcaagtcgat gcaggtaaaa atagacaggc
     2281 tcaaccaggt agtccgcgag aagcttgccg gagtccgggt catcagagcc tttgttaaaa
     2341 ccgactacga agaggcgcga ttcgatgagg ccaacctcga tctcacaacc acagccttga
     2401 agatcaacag gatggtcgcc atcaccatgc cgctgctgat gctcatagtc aacatctcca
     2461 tggcagccat tatgtggttc ggcgcacaca gaatcgacag cggggccatg cccataggaa
     2521 accttacagc cttcatcgcc tacaccatgc aaatcctcat tgcggtgatg atggccacag
     2581 tgatgttcat catgatcccg cgggccagtg cctcggccga acgaatcctc gaagttctcg
     2641 agactgagcc gtctatctcc gcccccaagg cgccggtcgc gctcaaccca ggcaccgccg
     2701 actccgacgc gagcgtcgaa tttagggatg tagagttcac atacccgggc gcagagactc
     2761 cggtcctgcg cggaatctcc ttcatcgcct atccgggcga aaccaccgcc atcgtcggcg
     2821 gcacaggatg cgggaagtcc actctgatca acctcatccc ccgcttctac gatgtgaccg
     2881 gcggcagctt gctcatagat ggagtagaca tacgggaaat acctgaggag gacctgagga
     2941 acaaaatagg ctttgtccca cagaaagcct tccttttcag cgggacaatt gccagcaact
     3001 tgcgctacgg aaagcgcgat gccaccgacg acgaactctg gcatgccctg cgcatagccc
     3061 aggcagccga cttcgtcgcc gagatgcccg gacagcttga agccccagtc tctcagggag
     3121 gaacgaactt ctcgggaggg cagaagcagc gcctagccat cgcgcgagcc ttggtgaaaa
     3181 ggccgaagat atacgtcttc gatgacagct tctccgcact tgacttcaag accgacgccg
     3241 cacttcgcgc cgcgctcaat gacgaagtgg atgacgccat agtcttcatc gtggcgcagc
     3301 gtgtgagcac tatcatgaat gccgaacgca taatcgtgct cgacaaaggc gagatcgtgg
     3361 gaatggggac acatgaggaa ctgatgaagc tctgttccgt atatcgtgag atcgtcctct
     3421 cacagctttc agaggaggaa gtcgcatgag tgaagagaaa acagcagcac gacgccagcg
     3481 aggacatggt ccccgaggcg ccatgatgcc cggagaaaaa gcgcgcgact tcaaggggtc
     3541 gttgaagagg ctgttcgggt atctcaaacc gcacaagacg cagctcatca tcgtcatggt
     3601 tttatccata gcctgcgtcg tcctgaccat tgctgggccg aagatcctgg gcaaagccac
     3661 gaatatcctc tttgaaggag tcatcgggaa acagcttcct cctggacttg gcaaggaaca
     3721 gatcattagc atgctcaagg ctgccggcaa ggaccagatg gccgagatgg tggcgggaat
     3781 gaacatcgtc cccggcgccg gcgtcgattt cgaggccatc aagcgaatac tgctcatact
     3841 tgtcggaata tacatcgcca acgccttgtt cagttggctg cagcactaca tcatggccgg
     3901 aatctcccag cgcaccgtgt acagcctgcg caaagaggcg gatgcaaaac ttgggaggct
     3961 gcctctgaaa tactacgaca gcaacacgcg gggagagatc ctgagccggg tgacaaatga
     4021 catagacaac atcgccagca cgcttcagca aagcctgacg cagctggtta cggcgacgtt
     4081 tacggtaatc ggcgtgttga taatgatgtt ttccatcagc ccgctgcttg ccgcaattgc
     4141 tctggtagta ttgcccttgg gcgttatcgt cacgctgttc atcgccaaac gctcccagaa
     4201 gcagtttgcg gagcaatggg acagcaccgg cgcgctcaac ggacatatag aggaaatgta
     4261 caccggccat cggatagtaa aggccttcaa ccgccagcag gaggcgatcg acgagttcga
     4321 taaggaaaac gaccgcctct accaggccag cttcaaggcg cactttatct caagcactat
     4381 ccagccggcc atgcagttca tcaataacct aaactacgtg gcaatctgcg tgataggggg
     4441 tatgaaggtc gccaatggcg tcctctccct cggagacgtc caagccttca tccagtattc
     4501 aaggcagttt actcagccga tagtccagac agctagcatc atgaatgtgc ttcagtcaac
     4561 agtggcctca gctgagcgcg tgttcgagct cctcgacgag gaagaggaga cgcctgacac
     4621 cgaaaaccct gtagtcctgg aaaaggtaga aggccatgtg gtcttcaaga acgtctcgtt
     4681 tagatataag cctgacgtgc cgctgattga ggacatgaac ctcgaggtta agccaggaca
     4741 gacagtagcc atagtcgggc ccactggcgc tggcaaaacc accgtagtca acctcttgat
     4801 gagattctac gagctggatg ggggaagcat ctccatagac ggcgttgaca tccgcgacat
     4861 ggcccgaaac gacctgagga gattattcgg gatggtcctt caggacacat ggctcttcaa
     4921 cgggaccata cgcgacaaca tcgcctacgg gcgcgaggat gccaccgaag aggagatact
     4981 ggccgcggcc cgcgcggccc atgtcgacca cttcgtcagg actctgccta aagggtacga
     5041 taccgagctg ggcgatgagg catccaacat atcccaaggg cagaaacagc tgctcacaat
     5101 cgcccgagct ttcctcgccg accccgatat cctcattctc gacgaggcta caagctcggt
     5161 ggatacccgt accgaggtac tgatacaaga ggccatggca gagctgatgc gtggcagaac
     5221 aagctttgtt atagcccacc gcctctcgac aatacgcaat gccgacacta tcctggtgat
     5281 ggataatgga cgcatcgtgg agcagggcaa ccatgaggag ctcatggcag cccgcggatt
     5341 ctactacgac ctctacaaca gccaatttgc cggggcgtac gatgaagcgg tgtaagcttt
     5401 aaggtaaagc ataaaaggaa caccttcggg gcgcactgcc ccggaggtgt tccttttctt
     5461 taatcggtcc acccgaccca acgctctgtt gccctgttat catcaaaatg aactttatct
     5521 atcagacatc cgtctcctcc agtcatgtat atgcaatata tcttgcacaa gaaggaatct
     5581 tgcaagatat gttgaatatg agctttagcc catgtcggcc attctccaac aggatggtga
     5641 attagtgtgg aaaacctcgg gattgtcact gagcgaatcc attcaaaata tgcttccatg
     5701 accaagagtc agcagcgtct cgccgacttc gtactgcaaa acatgccaga agtcgccatg
     5761 atgtccagtg ctgatctcgc ttgttgcatg gatgtcagcg aggccacggt ggtcagattt
     5821 gcccagatgc tcgagttcaa cggatacccc gacatgcgac agcagctaca aacagaactg
     5881 ctgaagcagt ttcgctcatc cagcagagtt gctgcaatgg tccacgcaca atcggataat
     5941 gcaggtccat tgaccatggt agtaagtgaa accataaaaa gcttgcagct tctccttcaa
     6001 aacgtcaatg atgcacaagt tgaagaagca gtgcagagaa taatcagcgc tcggcgagtg
     6061 attgttttcg gcgagggcgc tccggccagc attacagtgc ttgcggattt ctggctatcg
     6121 cgccttggtt gccaggtagt ccgcgtaaac cagaccggaa ggcgtctgtt cgacaagata
     6181 ttccaagcag gatcggaaga tctggctata gtttttgctt tccgccgggt aaccttagaa
     6241 gccatggcta tacttgaaca cctcaatcaa gaaggtggac aaagcctgct cgtcaccgat
     6301 ctcattgatt ccaaaatgca tcctctggca acccaagtat tccttgttca acgcggtccg
     6361 atggaggcct tcagacccat gggggccccc cttgcgttgg tagatgcatt gatcctcggc
     6421 gttatgctcg caaaaggcga tgatgctgta gagcagctca gtcgcttaga tacattacgc
     6481 caacgctatg actgtctatg attatcaaaa ataggaggga atcgtatgag tcaacggata
     6541 atcaagttag taatcttgtg cgtattcgtt ggaatgattg gagcgtacgt tggggcacca
     6601 cgagcctatt ctgcccccaa acttcctgct gatgtggatg catggcttaa gcaagttgag
     6661 atcggcccct accaacctgc taaggtagat tgggacaaag tttaccaagc agctaaacgc
     6721 gaaggcaagg tggtgatcta cacaagttcc tcgcgaacga tcagcttaaa gtcagagttt
     6781 gaatcactct atccgggcgt gaccatggaa gtatacgagc tgggaaccac aggcgccatc
     6841 gagaaggtcg aacgagaaca attagcaggc gtatacaatg ctgacatact tcatgcctca
     6901 ggttatgcgg accaactcca tctcctctac aacaagaaca tgttgttccc gttctacccg
     6961 cccgagctcg aatccgtgat cccgcgggca tttcgcgagc ccttggttgc ccagcgatac
     7021 gagtcgcgaa ccatcttcta taattctaag gtctacccga aatgccccat caacagctgg
     7081 tgggatctga ccaagcctga gtggcgcggc aaaatagcaa tggttgatcc tgtgaatgat
     7141 gcatctagtc tggacctgat cacaaccata gtgctcaacg ccgacgatct agcccgtgat
     7201 taccagcgag tgtttggacg ccccatccat ctcaccacgc caaatgcagg atacgaactt
     7261 ctcaaaggca tcatcgcaaa caaacctcgc atatactctc gacacacaga tctaataaac
     7321 gtagtgggcg atcctactaa caagaatccg ccgattggga tatcaatccc cttttccagc
     7381 ctaggatacg ccgaagaccc agctagaggc agcctcaaac tcatggcgac gactaatatc
     7441 acgcctactg tggggctact atatcccagc cttatgaaca tcacatacaa ggctccgcat
     7501 cctaacgctg ctaaactgct gatcaaatac ttgttcggag atcaatacgg gggacttggc
     7561 atgaaaccgt tctttatagt gggtaattgg ccagcccgca ctgacatgct ggccgagccg
     7621 aagaaccagt ttttgcccaa tctttctttg cctctaaaga gcatgaagtt ctggatgctt
     7681 gacccgatcg ggatatggaa aacttcggcc gaggttcagg aatggtggat gataaacatc
     7741 aactaatgga caactaccaa gaaatctgaa ggctaaggcg gaggcgcccg tcttgggaac
     7801 tatccgcctt agccaatgta gcactggcgg aggttgagcc aatgaacaat gtgaaattac
     7861 acggtcgcaa ggcatggaat cagcttcgta agccagaatc gatcttatcg atcttgttct
     7921 tgatcatttt ggcttaccta ataatagttc cgctcatctc actcatatgc agaacctttg
     7981 tatgggggac tggcgatctt cgcatccggg gtgaaaatgt agagcctggc aaattcactt
     8041 tgtatcactg gcgacgcatg ctaactggag atttcgcaaa atctgttttc tacaaaccat
     8101 tgctcaatac tcttacaatc actgttggca ccgtagccat catactgctt ataagcactc
     8161 tcctggcata tctagtagtg cgcactgaca ttcggtgggc taagcttatc aacgccttag
     8221 ccattgtacc ttatatcctc ccttcttggg tattgtcgtt gtcttggcta cagatcttcc
     8281 agaacagtgc tgttgcattg ccggatggtc tacttttctt cctcacaggc aaaaatgctc
     8341 ctgagtggct ggtttacggt ccgatcccca taatgattac cttgggcttg cactactatc
     8401 cctttggcta tctattgata tccggagctc tgtctactgt agattctcag ctagaagagt
     8461 cagcagagat cctcggcgcc aaccaacggt tcataaggcg tcacataacc atgcccatag
     8521 taagtcccgc cttgctttcc actgcattac taggcatagc gcgttccatg ggcactttcg
     8581 gaactccggc aatccttggc ctgcctgcta gatactatac tttatcgacg cagatatact
     8641 ctctcattca cactcaaaga gaagcacaag cttacatact agcaattgtt ctgctgttga
     8701 tatccagcac tggcctgtat ctcaaccaga agctcatcag ctctcgtcgc agttacgcca
     8761 caataggggg aaaaggagta aagtcgcgaa aagtaaagct gggaaagtgg aatacgccca
     8821 tagcagtgat cactttgata tttctgttca tggtatccgt gttccctctg ggcttccttg
     8881 tatggagcag tttctgcctg aacgcaggcg actacagcat atccaatctc tcgtcgcatt
     8941 actggatagg cgccagcaat cccaggtttg ctgctggtga accgggtatc ctccgtaacg
     9001 aagcagtacg gggagcggct tggaacagcg tccgcttatc acttatttca gcgttgatat
     9061 gcggcctatt cggaatgctg attggctatg tcgtagtgaa atcgcggaac tcaccgatcg
     9121 ctcacatgct tgaacagatc tcattcgccc cgatgctcgt gccaagcata gtctttggag
     9181 ccctgtacct gactttgttt gccgtgcgac gaggcccgat ccccgcactc tacggcacat
     9241 tcactttact agttctcgtt accattgcca agcaattgcc ctataccgtg cgatccggcg
     9301 tatcatcgat gctccaagta tctcacgagc tagaagaggc agcagtaatt cttggcgcgt
     9361 catggagaga gcgcatgcgc cggataatgc ttccgctaac aaagagtggc tttgtgtctg
     9421 gtgcgctcat agtccttatc accagcatgc gcgaactcag tctatacata ctgctggtta
     9481 cgcccgccaa tcgcatgctt actacactta cttacaggta catagaagtg ggctacacgc
     9541 aattcagcaa tgccatgacc gtgcttctca cccttctggt actattaatg acctgggcga
     9601 ttaaagcttg gcaaaacatg gatattgctg aaggcttagg aggcatgtag atatggaagc
     9661 agtcaaactg gtcggcgtaa ccaaggaatg gccaggagta aaggctgtgg acaacctcag
     9721 tttcgacatc ggcaaagggg atttcgctac cttgctcgga ccgtctggct gtgggaagac
     9781 caccacgctc agaatgattg cagggctcga agatcccgac gctggagaca tatacataaa
     9841 cgggaaactt gttttctccg caagtcaggg aatcaacctt cctccagccg cccgtggcct
     9901 ggggataatc tttcaatctt acgctctgtg gccacatatg acagtctttg acaacgtggc
     9961 attcggtctc gttcagcaaa aaatgccacg cgataaaatc gaggccaagg tatttggcac
    10021 tctgaagcag gtagggttag aagacctagc cgatcggtat ccatcagagc tttcaggagg
    10081 ccaacagcaa cgcgtagcac tagcccgcat ggtaatagtc gaacccgagc tgcttctgat
    10141 ggatgagccg ctatcaaatc tggatactcg actcagaatg cagatgcgag caagcctgaa
    10201 acggatgcat caggaggcaa acaccactgt agtctacgtg acgcacgacc aagaagaggc
    10261 gctcacgctt tcaaccaaga ttgccgtgat gaaccatgga cagcttgagc agtattcgcc
    10321 cccagaagac gtatatgaac gtcctgccac gcatttcgtc gcggagttta ccggcaaccc
    10381 tcagactaac tttctagacg caaaggtcgt tcgcaacgga aactcacctt acctggctgt
    10441 aggtgataca gtactttggc cagctcaacc tggttggcca gaccaagttg tgctagccat
    10501 aaggcctgag ggcctgactc tttgcaacga taatcagccc ggtcggattc cagctgcggt
    10561 atacgcaaca ttgccagttg gtccatcggt gctcgtatat gccgactcaa acggaatcga
    10621 gatcgttatt cgtgccgaca gaggcacgtc attccaaggg aaccagcaag tctggcttga
    10681 gttggaccgc tcacagatca acatctatga tgcagaaaca gggaagagaa ttggggatga
    10741 cacagatcac tgacaagaac aacgttatca gagacatcaa aggtttcatg tttgacctcg
    10801 acggagtgtt gtggcttggc gaccatccca ttccaggcgt agcgaatacg cttaaacagc
    10861 tcaggagaga aggcaaacaa ctcatgtttg tctccaatac cagctcgcgc tcaaaggcga
    10921 agtgcctttc aatattcaag cacatgggtc tggacgttga cagcagcgag gtattcatag
    10981 caagcgaatg tgcagcaaag tatgtggcaa gtcagatgcc aggcgccata acatacgtgt
    11041 tgggatcgga agggcttctg gaagaggcgt tagccgctgg tctggatgcg cgtaatgcct
    11101 caaccagaaa tcctgaaaaa gcccactacc tcattgccgg caaggataat gatctaagct
    11161 ttgctcggct cacatgtgcc ctacgcgttc tccagtcagg tgcacgtttt gtagccgtga
    11221 attgtgaccc aacagttccc tcgcaagacg gcttggaacc aggagcgggg gcaatagtag
    11281 cagctataac ggccatggtt aatcgcgatc ctgacgtcat cgtaggaaaa cctggaaccc
    11341 tgttgctgga aatggccttg gaacaaagcg ggctcgacgc agcggagtgc gccattgtag
    11401 gcgacactct tgagacggat atccttgccg ggaatcgatt ggggttgagc accattctcg
    11461 tgctcagcgg aaattccaag ggcctcgaca tgagcgctcc agatgcttac ccgatagaat
    11521 tccgacctga cattgtcatg aattctgtag aggatctagt ctattgagcc aataaccgtc
    11581 agatgagtac tgactcgaac ctcgatttct cgcgcaactg ccgcttcgcc caggctaact
    11641 gccagggcga agcgtttttt atcctgaaga tccatcccca ggtggacgca gaatcaagag
    11701 cccaatagct tgagatgaat atcaaatcgg ccgaagagca gtaagcgcac cttttcctcc
    11761 gcaagctcca gcccgaattt cccccatacg ttccaccaac gctcgataaa accgttctgc
    11821 atcctgcttg tgctcgaagc agaccacgaa gctatccgca aacctgacca gatatgcctc
    11881 gccccggact ccctaatcgc tggcgctatg ctggttcatt tgcccccagg tttccttttt
    11941 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagaaat tggcctcaca
    12061 atagaaaacc gggctcaaat atggatccac tgaacttgtc tgcctgacag agcgtgtggc
    12121 ccccgtaatg gctagcctat taacctgcac ctgcgcttca ccattgacgc cacagcctcc
    12181 gtctgctttg tcaaaagtgt cagcttgttt atagatgcca cggatactct tgacgaggta
    12241 ttttctatga gcaaacatga tgcggtaaag agagtggtct acgagtggac cgcagacggc
    12301 ctcagagatg aagataatct tccggctgat tcccgaagca atgctctgca tggacagcgc
    12361 actccacttt cacgggtata ttgaccaaac ccctgacacc tgatcaacaa aacgaaactt
    12421 aagcttgaat accccgctct aaaggcatac ttcgttaagc cccgtctcat caacattggg
    12481 ctttccgagc aggtggtcaa tggcacgacg ggccgtctgt gatgcaatca ggcattcaac
    12541 aaaaatggat gcagaaatct gtgctcaggc aattcagaac tatgtcaggg atcccaacaa
    12601 ggacgtcaat aggcgcatgg aatatgtctt tagactcaag atccagagga aagtcaaaaa
    12661 cccaacaggg gtgtggctgt gatgcgacac aatctagtca tcggcagata cgccggaaaa
    12721 cggatcaagc ctcaaatcat gtaggccatt tcagacgcac tttgagcccg ttaagatgag
    12781 tgtacggcag tagcccatca tgttgcagac gtccgacaac aattcccggg gccacgccga
    12841 ttgacttggc aaatgctgaa accttgttcc gacttattcg ccctccctga atgccttcgg
    12901 taaacttgcg atactcagtc ggcggcacga ggatgtcggc agcgaagcga tccgcctcaa
    12961 gctctacttc tgatgcgaga tcgttgggtt cgaaattgtc aatgaatgca ctgctctggt
    13021 agccgcccag tacgtgggct gcttcatgga aaaatgtaaa ccaaagttga tcgtcggtct
    13081 tgtagcgaaa gctcagct
//
LOCUS       DAOSYL010000065        11967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_4526_length_11967_cov_7.946944, whole genome shotgun sequence.
ACCESSION   DAOSYL010000065 DAOSYL010000000
VERSION     DAOSYL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_11967_cov_7.946944"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..4059)
                     /gene="rpoC"
                     /locus_tag="PLU15_09170"
     CDS             complement(235..4059)
                     /gene="rpoC"
                     /locus_tag="PLU15_09170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOO31128.1"
                     /translation="MLDVNDFDRIRIGMASPEQIRAWSSGEVKKPETINYRTLKPERE
                     GLFCEKIFGPTRDWECHCGKYKRIRYKGVVCDKCGVEVTRAKVRRERLGHIELAAPVS
                     HIWYFKGIPSRMGLLLDMSPRSLEKVLYFASYIVTDPGDTPLSKKQLLTESEYREYRE
                     KYGQLFKAGMGAEAIQELLRDIDLDELVQELRDDIENSSGQKRVRAIRRLEVAEAFRK
                     SGNRPEWMILDVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLDLEA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSMSDLLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPTLKMHQCGLPKEMALELFKPFVMKRLVDKGYAHNIKSAKRMVER
                     VRPEVWDILEEIIAEHPVLLNRAPTLHRLGIQAFEPVLVEGRAIQIHPLVCTAYNADF
                     DGDQMAVHVPLSAEAQAESRLLMLSVNNILSPAHGGPLATPTQDMVLGCYYLTFERIG
                     AKGEGKLFENPEAARAAYDAGAIALHSMIVVRIPESKLLLGAEEAGTRRIVTTVGKLI
                     FNEIFPDDFPYINSAESSFDSALTEGDFLPRGVSFWEYEESGGNRMRKPATKGFLSKL
                     VGVIRRKYGNARTADVLDALKNLGFHYATVSGTTVGIVDIAVPDKKQAIIKDADARVE
                     EIEGQYRRGLLLEDEKYSLIIDVWTEARENVQEAMMREIRPDILMARKLEEAKLTGER
                     ERVEFKIDELNPVYMMATSGARGNVQQLNQLAGMRGLVADPSGRTIDVPIKANFREGL
                     NVLEYFISTHGTRKGLADTALRTADSGYLTRRLVDVAQDVIVREDDCGTTEGIDVSAI
                     VEGDTVIESLSERITGRVSLQELVNEETGEVIVAKNQMIDEDQAKMIEDLGYESWAVR
                     SVLKCRTRHGVCVMCYGRNLATGNLVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVA
                     GDDITRGLPRVEELFEARKPKGLAIITEYAGEVSMVEAKGSRKIVITQPSGESHEYSV
                     PYGARLEVKEGQKVEPGQKLTEGSLNPHDILRIRGSVAVQQYIVREVQDVYRSQGVEI
                     NDKHIEVVVRQMMRKVKVENPGDTDLLPGELVDIFRFEDTNAEAKANGLRPATCKPAL
                     LGITKASLQTDSFLSAASFQETTRVLTEAAMKGKSDELIGLKENVIIGKLVPAGTGMP
                     RYRNVRVLASPEASREIERDIEKAERGRRDVREAASPGAARADSLGAPLAEHLGKEIT
                     EALSEGASQAQS"
     gene            complement(4116..7790)
                     /gene="rpoB"
                     /locus_tag="PLU15_09175"
     CDS             complement(4116..7790)
                     /gene="rpoB"
                     /locus_tag="PLU15_09175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOO31129.1"
                     /translation="MDIAERPMRRERISFSRIADVLEVPNLIEIQQKSYQWFLEEGLL
                     ETFRDISPVQDFTGNLVLEFTGYALGDPKYTEEECKQRDVTYSAPLKVKVRLINKETG
                     EVKEQEVFMGDFPLMTETGTFIINGAERVIVSQLVRSPGAYFDVDKDSSGRTIYKASI
                     IPNRGAWLEFETDASDIVWVHVDRTRKLPVTVLLKAIGYGSAQEILELFGENELIRTT
                     LERDNTNNEEEALIEIYKRLRPGEPPAIESARSLFESLFFDPKRYDLAGVGRYKLNKK
                     LELADRLIGHVLARPLYGDIVENTNTVRTVIDPETGEISWLDMSDGGIDSGRKPALEA
                     GEKITARRAELVRELEENVHIREASLVLPDGDPQDFIKVIGNGYPPADLKVITPADIV
                     ATVNYLVNLPYGIGTIDDIDHLGNRRLKTVGELLQNQFRIGLSRMERVIRERMTIQDV
                     EAVTPQALINIRPVVAAVKEFFGSSQLSQFMDQTNPLAELTNKRRLSALGPGGLSRER
                     AGFDVRDVHHSHYGRMCPIETPEGPNIGLIGSLCTYARINEYGFIETPYRKVEDGRAT
                     AQIRYLTADVEDRYIIAQANEPLNEDGSFVSPRVACRLKDEIVIVAADQVDYMDVSPK
                     QLVSIATALIPFLENDDAGRALMGSNMQRQAVPLIKTEAPFVGTGMEYKSAVDSGVVL
                     VAKRGGYVRHVSADHIVIERDDGVEDRYNVIKFARSNQGTCMNQKPIVKIGQRVEAGD
                     VIADGPSTDMGEMALGRNVLIAFMPWEGYNYEDAILISEKLVSDDVFTSIHIEEYECD
                     ARDTKLGPEEITRDIPNIGEDALKDLDEEGIVRVGAEVRPGDILVGKVTPKGETELTP
                     EERLLRAIFGEKAREVRDTSLRVPHGEAGKVVEVHVFSRENGDELAPGVSKLVRVYVA
                     QKRKVSEGDKMAGRHGNKGVIAKILPIEDMPFLPDGRPVEIVLNPLGVPSRMNIGQVL
                     ETHLGWAAAALGVHMATPVFDGAHEEDVFEYLEKAGLPRNGKTILYDGRTGEPFDREV
                     TVGYAYLMKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQELLTVKSDDIVGRVKTYEAIVKGDNVPEPGVPESFKVLIKELQSLCLDVKVLS
                     EDEEEIEIRELDDDIEGTARELGLDIGNRSGRLESGGAAASTRVGKTLDEDGEDEPDD
                     SDDEDQLDAEDEGDSDYDAD"
     gene            complement(8216..11647)
                     /gene="rpoB"
                     /locus_tag="PLU15_09180"
     CDS             complement(8216..11647)
                     /gene="rpoB"
                     /locus_tag="PLU15_09180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOO31130.1"
                     /translation="MKRERILFSKVPSVLELPNLIEVQHKSYQWFLGEGLREVFRDIS
                     PVQDFTGNLVLEFTGYALGDPKYTVEECKRRDVTYSAPLNAKVRLISKATGEVKEQEV
                     FMGDFPLMTEAGTFIINGAERVVVSQLVRSPGAYFDIDRDSSGREIYTASIRPNRGAW
                     LEFETDASNMVWVRVDRTRKLPATVMLRAMGLSSDEEIIEVFGDNEYIRNTLERDNTS
                     NEEEALIEIYKRLRPGEPPSIDSARSLFEFLFFDPRRYDLAKVGRYKLNKKLELADRL
                     IGQRLAEPIHHPVTNGTDPARAVVDSETGEITWLDDAGEEVDLGTKLALEAGEKVTAR
                     RAELVRELEQQGHIGEAILVAPDDAPQDSIKVIGNRYPPANLVVITPADVVATVNYLV
                     NLPYGIGTIDDIDHLGNRRLKTVGELLQNQFRIGLSRMERVIRERMTIQDVEAVTPKG
                     LMNVRPVVAAVREFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLSWRRAGFEVRD
                     IHHSHYGRICPIETPEGPNIGLIGSLCTYARVNEYGFIETPYRRVEDGKVTEEIRYLT
                     ADMEDRYVIAQANEPMNEDGSFASQRVACRLKDEIVIVPVDQVDYMDVSPKQLVSIGT
                     ALIPFLGNDDAGRALMGSNMQRQAVPLLSPEAPLVGTGMEYKSVLDSGAVIVAKHGGF
                     VRSACADRIVVERDDGRLDTYNVTKFARSNQGTCINQKPLVRVGHRVEPGDVIADGPS
                     TDMGEMALGRNILVAFMPWEGYNYEDAILISEQLVIDDVFTSIHIEEYECDARDTKLG
                     PEEITRDIPNIGEDALKDLDEDGIIRIGAEVQPGDILVGRVTPKGETELTPEERLLRA
                     IFGEKAREVRDTSLRVPHGESGKVVEVHAFSRENGDELAPGVGKLVRVYVAQKRKISE
                     GDKMAGRHGNKGVIAKILPVEDMPFLPDGRPVEIVLNPLGVPSRMNIGQVLETHLGWA
                     AAALGVHVATPVFDGADEDDVFGYLEKAGLPANGKTILYDGRTGEPFDREVTVGYAYL
                     MKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEM
                     LTVKSDDVLGRVKTYEAIVKGDMIPEPGVPESFKVMIREMQGLCLDIQVLPADEE"
BASE COUNT         2585 a         3725 c         2750 g         2907 t
ORIGIN      
        1 cgctcctttc ggcatatcgt ttcaagccct agcttcagag gctcagacag ttttacattt
       61 taccatcagc ccccgagcct gtcaaggggg cacaacgagc tattccccaa ttctgcgaga
      121 tttactccaa cagaaccgcc ggttccaaga agcgaccgca cgcccacgca aaactcacgc
      181 gaacatgcgg ccacacaacc tagcctagcc gagagcgtaa gatagccggg ctgtctagga
      241 ctgggcctgc gacgccccct cgctcagggc ctctgttatt tccttcccca gatgctccgc
      301 caagggcgct ccaagagaat ctgccctcgc ggccccagga gatgcagctt ctcttacatc
      361 tcgtcgacct ctttcggcct tctcaatatc gcgttcgatt tccctactgg cttccggaga
      421 cgcaaggacc ctcacattgc ggtatctcgg catcccggtg cctgccggaa caagcttgcc
      481 tatgatgaca ttctccttta gaccaatgag ctcatcactc ttgcccttca tagcagcttc
      541 cgtaaggact ctggtagtct cctggaacga agccgccgac aggaagctgt ctgtctgcag
      601 tgacgccttg gtgatcccga gaagcgccgg cttgcacgta gctggcctga gcccattcgc
      661 cttggcttca gcattagtgt cctcaaacct gaagatgtct accagctcgc cggggagcag
      721 atccgtgtct cccggattct ctactttcac cttgcgcatc atctgtctga ctactacctc
      781 gatatgcttg tcattgatct cgacaccctg ggaacggtag acgtcctgca cttctctgac
      841 tatatactgc tgcacagcta ctgacccgcg aattcgcaat atatcgtggg gattgagcga
      901 cccttcagta agcttctggc ccggctcgac cttctggcct tccttaacct ccagccgtgc
      961 accatatggc acggagtact catgggattc gcctgaaggt tgagttatga ctatcttgcg
     1021 agaccccttg gcctcaacca tgcttacttc gccagcatac tcagtgatga tggcaaggcc
     1081 cttgggcttt ctcgcctcga acagttcctc aaccctgggc aggccgcggg tgatgtcgtc
     1141 ccctgcaacg ccgcccgtgt ggaatgtcct catagtcagc tgcgtgccgg gctcgccaat
     1201 ggattgcgca gcaatggttc cgaccgcttc gcctacttca accaagttgc ctgttgcaag
     1261 attgcgcccg tagcacatga cacacacacc atggcgcgtc ctacatttca aaaccgaccg
     1321 cacagcccaa gactcgtatc ccaaatcctc gatcatcttg gcctggtcct catcgatcat
     1381 ctggttcttg gcgacgatca cctcaccggt ctcttcgttc accagctcct gcaggctcac
     1441 gcgcccagtg atcctctccg acaggctctc gatcaccgta tcgccttcaa caatcgcgga
     1501 cacatcgatt ccctcggtag tgccgcaatc atcctccctg acgattacgt cctgagccac
     1561 atccacaagc cgcctggtga ggtagcctga gtcagctgtt ctaagagcag tgtcggccag
     1621 ccccttacga gtcccgtggg tggaaatgaa gtattccaac acattcaggc cttccctgaa
     1681 gttggccttg atggggacgt cgatagtccg tcctgacggg tcggcgacga ggcctctcat
     1741 ccctgcaagc tggttgagct gctgcacatt accacgagcg cccgatgtgg ccatcatgta
     1801 tacgggattg agctcgtcga tcttgaactc cacacgctct ctctccccag tcagcttggc
     1861 ctcctcgagt ttgcgggcca tcagaatatc cggccgaatc tcccgcatca tggcttcctg
     1921 aacgttctcg cgcgcctcag tccaaacatc tattatcagg ctgtactttt cgtcctccag
     1981 aagcaagcct cttctgtact gaccctcgat ctcctcgacc ctggcgtcgg catcctttat
     2041 gatggcctgt ttcttgtcgg gcacagcaat gtcgactatg cctaccgtag tccctgacac
     2101 agtggcgtaa tgaaatccga ggttcttcag ggcatccaat acatcagcgg tcctcgcgtt
     2161 cccgtacttt ctcctgatca cacccacgag tttgctcaag aaacccttgg tagccggttt
     2221 gcgcatccga ttgccgcctg actcctcata ctcccaaaaa gagacacccc tcggcaagaa
     2281 gtcgccctca gtgagcgctg aatcaaatga tgactccgcg gagtttatat aggggaagtc
     2341 atccgggaat atctcattga atataagctt gccaacggta gtcactatcc tgcgagtgcc
     2401 agcctcctcc gctcccaaca gaagcttgga ctctggaatt cgcactacaa tcatggaatg
     2461 caatgcgatt gcgccagcat cataagccgc tcgagccgct tccggattct caaacagctt
     2521 cccctcgccc ttggcgccta tccgctcgaa tgtcaggtag tagcagccca aaaccatgtc
     2581 ctgcgtgggc gtagccagcg ggccgccgtg agccggtgaa agaatgttgt tcaccgacag
     2641 catgagcaac ctcgattcgg cctgcgcctc agcggacaac ggcacgtgga ccgccatctg
     2701 gtcaccgtcg aagtctgcat tgtaggcggt gcagacaagc ggatgaatct gtatggccct
     2761 gccctccacc aagacgggct caaacgcctg aatgccgagc ctatgaaggg taggagcacg
     2821 gtttagaagc accggatgct cagcaatgat ctcctcgaga atgtcccaca cctcgggcct
     2881 tacgcgctcc accatccgct tggcactctt tatgttatga gcatagccct tgtcgacaag
     2941 cctcttcata acaaaaggct tgaacaactc aagagccatc tccttgggca atccgcactg
     3001 atgcatcttg agagtcggtc cgacgacaat aacggatctg cccgagtagt cgacacgctt
     3061 tccgaggagg ttctgcctga accttccctg cttgcccttg agcaagtctg acatcgactt
     3121 caagggcctg ttgccaggtc ctgtaaccgg ccttcccctg cgaccattgt cgataagagc
     3181 gtccaccgcc tcttgcagca tccttttttc gttgcgaacg ataatgtcgg gcgcctccaa
     3241 gtccagaagc ttcttcagcc tgttgttcct gtttatgacc cgacgataca gatcattcaa
     3301 gtcgctggtg gcgaacctgc caccgtccaa ctgcaccatt gggcgcagat ccggaggtat
     3361 cacaggaatg acatcgagta tcatccattc aggccggttt ccggattttc gaaaagcctc
     3421 agcaacctcc aacctgcgta tggcacgcac acgtttctgg ccagaactgt tttcaatatc
     3481 gtcgcgaagc tcttggacaa gctcatccaa atcgatgtcg cggagaagct cctgtatcgc
     3541 ctcggctccc attcccgcct tgaagagctg tccgtatttc tctctgtatt cacggtattc
     3601 gctttcagtt aatagctgtt tcttcgaaag cggcgtatct cccggatcag tcacgatgta
     3661 ggatgcaaag taaagcactt tctccaatga cctaggagac atatccagca agaggcccat
     3721 tcggctcgga attcctttga aataccaaat atgcgagacc ggggcagcca attcaatatg
     3781 gcccaacctc tcgcgacgaa cctttgcccg ggtaacctcc acgccgcact tgtcacatac
     3841 gacgcctttg tatcttatgc gcttgtattt gccgcaatgg cattcccagt cacgcgtggg
     3901 gccaaagatt ttctcacaga aaagtccctc gcgttcaggc ttcagagtac gatagttaat
     3961 cgtctcaggt ttcttaacct caccactcga ccaggcccgg atttgctctg gcgatgccat
     4021 tcctattcta attcgatcaa aatcattgac gtccagcatc cgcccctccc cctccttcgc
     4081 cctggggctc cgacccgtta tccgccaccc gcatcctaat cggcatcgta gtcgctgtct
     4141 ccttcatcct cggcgtcgag ctgatcttca tcatcactgt catcaggttc atcctcgcca
     4201 tcttcgtcaa gggttttgcc caccctcgtg gacgccgccg cgccacccga ctcgagtcgc
     4261 cctgaccgat tgccaatatc gaggcccagc tctcgtgcgg tgccttcgat atcatcgtcg
     4321 agctcgcgaa tttctatctc ttcctcatcc tctgacaaaa ccttcacatc aaggcacagg
     4381 ctctgaagtt ccttgatgag caccttgaag gactcaggga cacctggttc aggaacattg
     4441 tcgcccttta caatagcttc gtaagtcttc accctgccta cgatatcgtc cgatttgacg
     4501 gtgagcaact cttggagggt ataggccgcg ccgtacgcct ccaacgccca cacctccatc
     4561 tcaccaaacc tctggcctcc gaactgtgcc ttaccgccca acggctgctg ggtaacgaga
     4621 gaatatggcc ccgtcgaccg cgcgtgaatc ttgtcgtcca ccaggtggag aagtttcatt
     4681 aggtaggcat agcccacagt cacctctcgg tcgaaaggct cgccggttct cccgtcatac
     4741 agaatcgtct tgccgttccg tggaagacca gctttctcca aatactcgaa cacatcttct
     4801 tcatgcgctc cgtcgaatac cggcgttgcc atgtgaacac cgagagcagc ggcggcccag
     4861 ccaagatggg tctcgagaac ctggcctata ttcatccggg aaggcacccc caacggattg
     4921 aggactatct cgacgggacg cccgtccggt aagaacggca tgtcctctat aggcagaatc
     4981 ttggcgatga cacccttgtt cccgtggcgg cccgccatct tgtcgccttc cgagactttt
     5041 cgcttctgag ccacatagac tctgacaagc ttgctcacgc ccggcgcaag ttcatctccg
     5101 ttttcacggg aaaaaacgtg gacctccacg actttccctg cttcgccatg aggcaccctg
     5161 agggaagtat cgcgcacttc gcgcgccttc tcgccgaata ttgcccggag caagcgctct
     5221 tcaggcgtca gttccgtctc gcctttcggc gtaacctttc ccacaagtat gtcgccgggc
     5281 ctcacctctg cgccgaccct gactatgccc tcttcatcca ggtccttcag ggcatcctca
     5341 cctatgttgg gaatgtcccg ggttatctct tccggaccaa gtttcgtatc acgggcgtcg
     5401 cattcgtatt cttctatatg gatcgatgtg aacacatcat cgctaacaag tttctctgat
     5461 atgaggatag cgtcttcgta gttataccct tcccacggca tgaacgcgat gagaacattt
     5521 cgccccagcg ccatctctcc catgtcagtg gatggtccat cggcaatcac atcgcccgcc
     5581 tcaacccgtt gaccgatctt gactatgggt ttttggttca tgcacgtgcc ttggttggaa
     5641 cgggcaaact tgatcacgtt gtatctgtcc tccaccccgt catctctttc aatgacgatg
     5701 tggtccgcag acacatgacg cacgtatccg ccgcgcttgg ccactagcac caccccggaa
     5761 tcgacagcag acttgtattc catccctgtt cccacaaaag gagcttcagt ctttatgagc
     5821 ggaacagcct gtcgctgcat gttcgaaccc ataagggcac gacctgcatc gtcgttctcc
     5881 aggaatggga tcaaagccgt cgcaatagag accagctgtt tcggggagac atccatgtag
     5941 tctacctgat ccgctgcgac tatcacgatt tcatccttca agcggcacgc cactctcgga
     6001 ctgacgaaag acccatcctc gttcaggggc tcattcgctt gagctatgat gtatctgtct
     6061 tccacatcag cggtgagata cctgatctgc gcagtcgccc ttccgtcctc tacctttcta
     6121 taaggggtct ctataaagcc gtactcgttg atgcgagcat acgtgcaaag cgaccctatc
     6181 agcccgatat tcggcccttc aggcgtctcg atcgggcaca tcctgccgta gtgcgaatga
     6241 tggacgtcac gaacatcgaa cccagccctt tccctggaaa gccccccagg gccaagtgcc
     6301 gatagccgac gtttattcgt caattccgct agcgggttgg tctggtccat gaactgcgac
     6361 agctggcttg atccgaagaa ctccttcacc gccgctacca ctggtcttat gttaatgagc
     6421 gcttgaggcg tcacggcctc cacgtcttgg atcgtcatgc gctcgcgtat cactcgctcc
     6481 atgcgtgaca gaccgatcct gaactggttc tgcaaaagct cgccaacggt cttcagcctt
     6541 ctattgccaa gatggtcaat gtcatcaatc gtgccaatgc catacggcag attgacaagg
     6601 tagttgactg tggccactat gtccgcaggc gtaataacct ttaggtcggc cgggggatag
     6661 ccatttccaa tcaccttaat gaagtcttgt ggatctccgt ccggcagaac gaggctagct
     6721 tcccgtatgt gtacattctc ctcaagctct cgaacgagct cagcccttcg ggccgttatc
     6781 ttctcccccg cctccaaagc cggcttgcgc cctgaatcta tcccgccgtc actcatatca
     6841 agccaggaga tttctcccgt ctcaggatca ataactgttc tgactgtgtt tgtgttctcc
     6901 acaatatcgc catacaaagg tcgggcaagc acatgcccga tcagtctgtc ggcgagttca
     6961 agtttcttgt tgagcttgta gcggcccacc ccagccaagt catacctctt ggggtcgaaa
     7021 aagagcgatt caaaaagaga tcttgcactt tcgatggccg gcggctcgcc tggccgaagt
     7081 cgcttataga tttcgatgag cgcctcttcc tcgttgttcg tgttgtcgcg ctccagagta
     7141 gtgcggataa gttcgttctc cccgaaaagc tctagaatct cctgagcaga accatatcct
     7201 attgccttca acaggactgt aacagggagt tttcttgtgc ggtctacatg cacccaaaca
     7261 atgtcagagg catcagtttc aaactcgagc catgcccctc tattcggtat tatggacgcc
     7321 ttatagattg ttcttccgga tgagtcctta tccacgtcaa agtaagcacc cggggacctc
     7381 accaactggc tgacgattac gcgctcagcc ccattaatga tgaacgttcc cgtctcggtc
     7441 atcaatggga aatcccccat gaagacttcc tgctctttga cttctccagt ttctttgttg
     7501 atgagcctga cctttacctt tagaggagcg gaataggtta catcccgctg tttgcattcc
     7561 tcttcggtgt atttcggatc gcctagagca taccctgtga attcgagcac aaggtttcca
     7621 gtaaaatcct gcaccggcga gatatctcgg aacgtttcga gaaggccctc ttcaagaaac
     7681 cattggtagg acttctgctg gatctcgatg aggtttggaa cttcgagaac gtccgcaatc
     7741 ctggaaaacg aaatcctttc acgcctcatc ggtctctcgg caatgtccat acaacccacc
     7801 ctacttcctt gtagattgta gatttccggg cataacgaat agcgcggaca ttaatgacct
     7861 ttggcgcata tcctccagtc aaagccgcgc tgagactaat agatcatacc tgcaaatagc
     7921 atgtgtgttc atttgtcccc ttatacctca gtcctccgtc actcagggcg tgcccgcgcc
     7981 gctccatctc atgaagggtt atgccccagc agatcacggc gcatcaactc tgctcatgcc
     8041 cttgtgacat tttattatgc taccacaaga gtgacgcccc gtcaagacca atttatctgt
     8101 tttttaagcg ctgatcaggt cttgttgata acaccgcctc tgctgtaaaa cgcctacctc
     8161 caaggcggac ccatgaatgt gaagattcca aaacccgcca catgcacgta attgctcatt
     8221 cctcatccgc aggcaagacc tgtatatcaa ggcataggcc ctgcatctct ctgatcatca
     8281 ctttgaagga ctcaggaacg ccaggttcag gaatcatgtc cccttttaca atcgcctcat
     8341 atgttttgac tctgcccaaa acatcgtctg actttacggt gagcatctct tgaagcgtgt
     8401 gggcggcccc ataggcctcc aatgcccaaa cctccatctc accgaatctc tgccctccga
     8461 actgagcttt gccgcccagt ggctgctggg tcactaggga gtatggtcct gtggaacgcg
     8521 catggatctt gtcgtcgaca aggtgtagta gtttcatcaa gtaggcgtag cccacagtca
     8581 cctctcggtc gaatggttca ccggttctcc cgtcatacag aatagtcttg ccatttgcgg
     8641 gcagacctgc tttctccaga tacccaaaaa cgtcgtcctc atcagcaccg tcgaacacag
     8701 gcgttgccac gtgaacgccg agagcggctg cggcccagcc aaggtgagtc tccagcacct
     8761 gaccgatatt catccgcgaa ggcactccca acggattgag gactatctcc accggacgcc
     8821 cgtccggcaa aaacggcatg tcttcgaccg gcaatatctt ggcaatcacg cccttgtttc
     8881 cgtggcggcc cgccatttta tcgccctcgg agatcttccg cttctgcgcc acataaactc
     8941 tgacaagctt acctacaccc ggagcaagct cgtctccgtt ctcacgggaa aacgcatgga
     9001 cctctactac cttccctgac tcgccgtggg gcaccctgag cgaggtatcg cgcacttcac
     9061 gcgccttctc gccgaatatt gcccggagca agcgctcctc aggcgtgagc tcagtctcgc
     9121 ccttcggcgt aacccttcct acaaggatgt cgccgggctg cacctctgcg ccgatcctga
     9181 ttataccatc ctcatccaag tccttcaggg cgtcctcacc gatattcggg atatccctcg
     9241 ttatctcctc aggtccaagc ttagtatctc gagcatcaca ttcgtactcc tcgatgtgta
     9301 tggatgtaaa gacatcgtcg atcacgagtt gctcggatat gaggatggcg tcctcataat
     9361 tgtagccctc ccacggcatg aacgcgacta aaatgtttcg tcccagcgcc atctcaccca
     9421 tatcagtgga tggtccatcg gcaatcacgt cgcctggctc gactctatga ccaactctga
     9481 ccaaaggttt ctggttaatg catgtaccct ggtttgatct ggcgaatttg gtcacattgt
     9541 atgtatccaa tctgccatca tcccgctcaa ccacgattcg atcagcacac gcacttctaa
     9601 cgaatccgcc atgcttggcc acgatcaccg ccccagaatc gagaaccgac ttgtattcca
     9661 tgcctgttcc caccagcggg gcttccggac tcaaaagcgg aaccgcctgt ctctgcatgt
     9721 tcgagcccat cagagcccga ccagcatcat cattccccaa gaacgggata agggcagtac
     9781 caattgaaac caactgtttt ggcgagacat ccatgtaatc tacctgatcg accgggacta
     9841 ttacgatctc atccttcaag cgacatgcta ctcgctgact agcaaaagaa ccatcctcat
     9901 tcatgggctc attcgcctgg gcgatgacgt atctgtcttc catatcggca gtgagatatc
     9961 tgatctcctc cgtcaccttt ccgtcctcaa cccgcctata gggcgtctca atgaaaccgt
    10021 attcattgac acgggcatac gtacacaaag accctatcaa cccgatgtta ggcccttcag
    10081 gcgtctcgat cggacatatc ctgccataat gagaatgatg gatgtcgcgc acttcgaacc
    10141 cggctctcct ccacgacagt cccccggggc caagcgctga caatcggcgc ttatgagtca
    10201 gttcggccag cggatttgtc tggtccatga attgcgacag ctggctcgat ccgaagaatt
    10261 cccttaccgc ggccaccact ggcctgacat tcatcaagcc cttaggcgtc acagcttcaa
    10321 catcctgaat agtcatgcgc tcgcgtatca cccgttccat gcgggataat ccaatcctga
    10381 actgattctg cagcagttcg ccaacggtct tgagccttcg attaccaagg tggtcgatat
    10441 catcaatcgt gccaatgcca tatggaagat tgacgagata attcacagta gccacaacgt
    10501 ccgcgggcgt aattacaaca aggttggcag ggggatatcg attgccgata acctttatag
    10561 aatcttgcgg cgcatcatcc ggagcaacaa gaatcgcttc tcctatatgt ccttgctgtt
    10621 ccagctcccg aacgagttcg gctcgtctgg cagtaacctt ttctcctgct tccaacgcca
    10681 gcttggtccc tagatctacc tcctcgcccg cgtcatcaag ccaggtgatc tctcccgtct
    10741 cggaatccac tacggccctt gccggatctg tgccatttgt aaccggatga tgtatgggct
    10801 cggccagcct ttgaccgatc agcctgtcgg caagttccag cttcttgttg agtttgtagc
    10861 ggcccacctt cgccaagtca tatctcctgg gatcgaagaa aagaaactca aacaaagacc
    10921 tcgcactatc aatagaaggc ggctcgcctg gcctaaggcg tttgtagatc tcgatcaggg
    10981 cctcttcctc gttactcgtg ttgtcccgtt ccaacgtatt gcggatgtac tcgttgtctc
    11041 cgaaaacctc gatgatctcc tcatctgagc taagccccat cgccctcaac atgaccgtgg
    11101 caggaagctt tctcgtccgg tcaacgcgca cccagaccat attcgaggca tccgtctcaa
    11161 actcaagcca agcgcctcgg ttaggcctta tggatgccgt gtagatctcc cttccggatg
    11221 aatccctgtc aatgtcaaaa taggcgcctg gggatctcac cagctggctg acgactacgc
    11281 gttccgcccc gttgataata aatgtgccag cctcggtcat gagcggaaag tctcccatga
    11341 aaacctcctg ttctttgacc tctccagttg ccttactgat caacctgacc tttgcattga
    11401 gtggagcaga ataagtaacg tcccgtcttt tgcattcctc caccgtatat ttcggatcgc
    11461 ctaaggcgta tccggtaaac tcgaggacaa ggttgccggt aaaatcctgg actggcgaga
    11521 tatctcggaa cacttcgcgc aggccctctc cgagaaacca ttgatatgat ttgtgctgga
    11581 cctcgatgag gttggggagt tcgaggacac tggggacctt ggaaaacagg attctctcgc
    11641 gtttcatcgg cttagtcgca atctcaatag accccaccct actttctaac ggcttttcag
    11701 atacaagaaa cagtgcggac catcatgaca tggaccaaac gcccataagc cagagccgca
    11761 ctgagactgg cagacctcac gtgcaaaaca tgagcgtttt gccgccctct cataacctat
    11821 tcactggaca cttgcgcgaa gccaaagctc ggctccgcaa gggctgggcg cccgcaaatc
    11881 acagcgcact cgatctgccc gtccttcggc atcatccttt atgttaacat atagcccgaa
    11941 tcgcgtcaaa tcatgccagc tagagcc
//
LOCUS       DAOSYL010000066        11451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_4813_length_11451_cov_7.547912, whole genome shotgun sequence.
ACCESSION   DAOSYL010000066 DAOSYL010000000
VERSION     DAOSYL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_11451_cov_7.547912"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(539..991)
                     /locus_tag="PLU15_09185"
     CDS             complement(539..991)
                     /locus_tag="PLU15_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO31131.1"
                     /translation="MENDAICIKRIDESLIDDCVSVYLEAFLQEPWNEKYEPESVEKY
                     FRDFMSGNICFGLALCEDNNVIGVLLGMILPSADGDYARIEDFCIHPKMQSMGYGSKF
                     LDLLKSYLSDSPVDCILLNTVRGFPSHKFYMKNGFTEIGTSVLLACNL"
     gene            complement(1099..2394)
                     /locus_tag="PLU15_09190"
     CDS             complement(1099..2394)
                     /locus_tag="PLU15_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO31132.1"
                     /translation="MQDPKQVARYGLDALLEAGADKAECSLTLTDKHELNVELGEFAL
                     MRTTFDTQVDLTAIRDNKKGSTSINKSNNDSLDRAVADVLDIAAASSPDDAYDIAEAQ
                     PPAAFSSGSDSPDVDRMHSRMKEMLESIKVRYPKLVLREAYLDFTRTRNYFLNSNGVD
                     FVTNKGVYTLVAMFSSRDGEKVSSFNYTAFSLKDLERDLLSCGSLDMLMQQSTEQINT
                     RPLEGKFVGDIIITPDCLGDMLHFLSYSIGDGPMISGTSIYKDKLDEQIASPLLTMHS
                     RPVSDEICNGYFVTPDGYAAQNSTILQDGVLKTFLLSLYGSRKTGLDRAVTSGGAFVV
                     DPGHTPLEEMVKSVKKGVLLARFSGGSPSDNGDISGVAKNSYLIEDGEIRYPISESMI
                     SGNVADMLMNVAAVSRERVDYGFSICPWVAVSGVTISGK"
     gene            complement(2397..3776)
                     /locus_tag="PLU15_09195"
                     /pseudo
     CDS             complement(2397..3776)
                     /locus_tag="PLU15_09195"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
     gene            complement(4225..4506)
                     /locus_tag="PLU15_09200"
     CDS             complement(4225..4506)
                     /locus_tag="PLU15_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOO31133.1"
                     /translation="MSRLTLMIDTNIIFSGIIWGGPEARLLDMALSGAFTLLIPDYVI
                     GEARAVVSRKMPAFLGILEQFLEQDFIVRSPHRQSSYESTIWNGVARIP"
     gene            complement(4499..4762)
                     /locus_tag="PLU15_09205"
     CDS             complement(4499..4762)
                     /locus_tag="PLU15_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31134.1"
                     /translation="MSTKGQLTIPSHIRERLALHPGDRFSCQVADGSAIIIKKLDPVE
                     VARLTIGEEFRRSGITPEQLEQLLEEVREGLSAQYMRDHYDDE"
     gene            5033..5707
                     /locus_tag="PLU15_09210"
     CDS             5033..5707
                     /locus_tag="PLU15_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31135.1"
                     /translation="MEILRAENVSKVYRGEHEVQALDQVNLSVEKGSLLAIVGDSGSG
                     KSTLLHILGGVDRPTSGRVFVQGTDITELHESQMSVFRRRNIGIIYQFFNLIPNINVR
                     KNICLPLLLDERVPDEDYYQEVIQTLGIADKQRRFPNELSGGEQQRVAIARSLINRPA
                     VILADEPTGNLDRRNTKEIVSLFKLVNKSFKATIILITHDEKVALACEKVCTMVDGRL
                     YEGDVR"
     gene            5704..8238
                     /locus_tag="PLU15_09215"
     CDS             5704..8238
                     /locus_tag="PLU15_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31136.1"
                     /translation="MSIIRELARDSVKTDRKASFAVSVSIFIAVVLVGFFLIIFSLID
                     YGQVEYMTETTGGYSAMLLAISPEDMAELKADHRVRSLAVIEREELKDAGLKRPQNEI
                     NYLDAHLYDYKWVELVSGRYPENAHELLVSELFLIENPGFEIGSDVTIADRHFYICGT
                     FKEYLYSFEGTYTSYGWIDSTKTQVMGPVDLCIWLENERAAYEHIPDLVQRLGRDPAQ
                     LKKDGGLIYNTAYLESKMIFPGGILKPSRDVAEKMLLRGIVLLCFVALFVMIVYNAYS
                     VWNDRDMKQIGLLKSCGMSESQVKRYVVCKAFYLSLWPILFGIIASYLLTLLLYYLFE
                     MTRAQYHIGYTPIELLKTPFAPLARAVLPTLCLALFTVYLASLKPAKKSSRLSVVSAI
                     KNLDPERNLKLKDIRYTSNIERSLAKEYSSNYSKTYRGMSIALAMACLISSAVLVEQS
                     FNHLDERYNTYESPYNLNAFLVTVDNVNPEMIERLRQVDGISRLVLYRDNGSLKFYES
                     DNPGFLSEEKTCARARLEEDKKAFDVPINLYGIDDAEWNHYCDEHGLKSSGRDAKGSY
                     QVLLLDRTCEDPGRPYLRSVHIPLAGEAISDIHLRRKTDTEAGSVSLKIVDRLTEVPF
                     GIGPMPRKSISLFMPLSCFNSFCQDNGFDQPLEGKAYMISITANEDMLDEITRATRQV
                     MYEYIPRSDARVESRNDIDRLSQEEAANSKILFIFIQSFLLLVGLSNAYNSFNSNLQA
                     RKRDFALLRSIGMEEKQLKGMLLHEGIFLIRRVLLIFLLLLMALVTLYAYRKRMLFAP
                     WEILANLNFPLLVAFLAINAIGIFAAIQGGTRRVMSQNILESLREE"
     gene            complement(8264..9463)
                     /locus_tag="PLU15_09220"
     CDS             complement(8264..9463)
                     /locus_tag="PLU15_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO31137.1"
                     /translation="MRQTVAGGAVAFIVSFLLLSLATGFALQEGEKALSLFAGAALIN
                     HSAEDTPGATETDLAIALKEKPDELIADGTRFLRNYGYLPQTSYINQHKLTWINLALA
                     LAAGLLVSFQSAREEKKLKRELLELSDYLRALHRGEDKLVLRDDLFGQLRDDIYKTIL
                     ILRESRENALADRALLKRNIEDITHQIKTPLTTIQLMLDLIEQDEANQAEYIARLDEE
                     IDRLNRLTSALLKLSSLDAGAIAFRSEIFSAENMIYEAIQPIECLIQQKQVDLRLTGQ
                     DYEIKGDRSWLMEAVLNLAKNAVEAVDDKGLVEIQLSENTIYQSITVRDNGPGIPAAD
                     LRHIFDRFFKSQNAQKESFGIGLSMAKSIVEGHGGSLEVESRSSGSSFEIRLYHELAA
                     AGADPSV"
     gene            complement(9460..10143)
                     /locus_tag="PLU15_09225"
     CDS             complement(9460..10143)
                     /locus_tag="PLU15_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO31138.1"
                     /translation="MKKILLVEDEENILFAVKRYLENAGYAVFPASTLSEARASLHDD
                     LDLILLDLNLPDGDGFAFLDLVRENSSIPIICLTVRDSDGDIIRGLDSGADDYITKPF
                     KLPVLKARIETVLRRVKKDPEFQSILQSDGVTLDKNLKKVFIDQREEELSLKEFQLLC
                     LFMENPGRTLTRELIIDIVWGLESDFVYDNTLSVNIRRLRHKLGHYQNKIQTVRGIGY
                     RWIEGEMSR"
     gene            10615..11004
                     /locus_tag="PLU15_09230"
     CDS             10615..11004
                     /locus_tag="PLU15_09230"
                     /inference="COORDINATES: protein motif:HMM:NF016309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HOO31139.1"
                     /translation="MHPDVMLLTQSGMWAFDAKFRLDRGSVSGHATKADTLGAMEDTA
                     RVDDIHKMHTYRDALTPGLRGAYVVYPGDEVLMYPAPGSSACNDRPGFRDGGPWVAVD
                     GVGAVPARPGSGMTGLFGVLDKILSLR"
     gene            11025..>11451
                     /locus_tag="PLU15_09235"
     CDS             11025..>11451
                     /locus_tag="PLU15_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31140.1"
                     /translation="MKEIVEEKRKRLGIVLCAIAIVMTVFGLIETNRSRKIVQILDNY
                     HRVDRIAINRGDVEAVVAGDELERYKEAFEPQVILSGDRKTINRVLGDKYLEMRFFIE
                     DELLFSAAIYELTTGSNVPDSFEMGSRRYIMKVGRDYRSL"
BASE COUNT         2687 a         2793 c         3007 g         2964 t
ORIGIN      
        1 agactgtctt gatttgtgcg tagataacta agttagtgaa ggtgaagcag cagtgaagaa
       61 aaattcgagc gtcatgacat cagcaggcgt gttgatatat gtgctattgt ctctaactga
      121 taggtttgtg gtaagcatcc cagaccacgt ttatattccg gttgcagtca taggcgctat
      181 ctttgtgctg gccggtattg ctgtaagccg gcgtacaaga tgagcagctg gcgcaaacgg
      241 gattgcccgg aaacgtctgc agaatctatg cgggagatgc ggaggcgaaa aacacgaaca
      301 atgtgatccc aactgaggga atgtagcatg gaggcgtttg tgttgaagtg ggaaaccctt
      361 aggtcgactg gatatgttgg ggagccccgt taacatattc cgagaggcag cttcattctg
      421 tgctttttcc tgctgttgtt cgtgcgtccg atgcaggaat gcgtctgaaa gatttcagag
      481 cccaggtgca gcagcccgag tgtttggcgt gggggtacgc gttgactgca ttgcctggct
      541 ataagttgca tgccagcaga acagaagtgc ctatttcggt aaacccgttt ttcatataga
      601 acttatgcga agggaagccc ctaacggtat tgagcaatat gcaatctacc ggggaatctg
      661 acaagtatga cttcagcagg tctagaaatt tgctgccata gcccatggat tgcatcttcg
      721 ggtgtatgca gaaatcctct attctggcat agtcaccgtc tgcagaaggt aatatcattc
      781 ccagcaagac cccgatcacg ttgttgtcct cgcagagagc aagtccgaaa cagatgtttc
      841 cactcatgaa atctcggaag tacttctcca cactttccgg ctcatatttc tcattccatg
      901 gctcctgtag aaaagcttcc agatagactg acacgcaatc atcaatcagg ctttcatcta
      961 ttctcttaat gcagattgcg tcgttttcca taatcacact tcccaagacc gatatagatt
     1021 ctgtctgctt tgcgcgagga tcggccggcc acttggcgtg gccggctgat ccttgtagac
     1081 gtcggtttgc tcaaagaatc atttccccga tatggtcact cctgagaccg caacccaggg
     1141 gcagatcgag aatccatagt ctacccgctc gcgggatacg gcagccacat tcatcaacat
     1201 atccgctacg ttgccggaga tcatcgattc gctaatagga taccttattt ctccgtcctc
     1261 aatcaggtag ctgtttttgg ctacgccgga gatgtcgccg ttgtcacttg gcgatccgcc
     1321 tgagaatctg gccaggagta cgcctttctt caccgacttg accatctcct caagcggggt
     1381 atgacccggg tctacaacga atgctccgcc gctcgtgact gccctatcaa ggcccgtctt
     1441 tctggaaccg taaaggctga gaaggaaggt cttgagtacg ccatcctgta ggatcgtgct
     1501 gttttgggca gcgtatccat ctggtgtcac gaagtagccg ttacatatct catctgacac
     1561 gggacgggag tgcatggtca gcagggggct cgcgatttgc tcatcaagct tgtccttgta
     1621 gatgctggtt ccggatatca tgggcccgtc gccgatggaa tatgagagaa agtgcagcat
     1681 gtcgccgagg cagtctggcg tgatgattat gtcgcccacg aacttgccct ctaggggccg
     1741 ggtattgatt tgctccgtgg attgctgcat gagcatgtcc agagacccgc agctcagcag
     1801 atccctctca agatctttga gcgaaaaagc tgtgtagttg aaagaagaga ctttctcccc
     1861 gtctctggat gagaacatag ccaccaatgt gtatacgccc ttattggtca caaaatccac
     1921 gccatttgag ttgaggaagt aattccgcgt gcgagtgaaa tcgaggtatg cctccctcag
     1981 cacaagtttc gggtacctca ccttgatcga ttcaagcatc tccttcatgc gggagtgcat
     2041 tctgtccaca tcgggcgagt cgcttccgga tgagaatgcg gccgggggct gggcttcagc
     2101 gatatcgtaa gcgtcgtcag ggcttgatgc ggcggctatg tcaagtacgt cggcgactgc
     2161 tctgtccaaa gaatcgttat tggacttgtt tatgctggtt gaacccttct tgttatctcg
     2221 tatggcagtg aggtcaactt gggtgtcaaa cgtcgtcctc atcagagcga attccccgag
     2281 ctccacattg agctcgtgtt tatcggtgag agtgagacta cattcagcct tatcagcccc
     2341 tgcctcaagc agggcatcta ggccgtatcg tgcgacttgt ttgggatctt gcatatctac
     2401 ctgcctccta tggtaacttt gcatttgatg gccggtccgc ccatgcctac aggtattggc
     2461 tgtttcttgc cgcacattcc gctgctcagc catttcatat caggcgatac catagtgacc
     2521 gtcttgagca tgtcaaaggc cacccctgag atagtcgtat ctcttatggc cctgccaatc
     2581 ttgccgtttt tgatctcata gccaagagtc acgccgaaca tgaactcgct ggtggagtcg
     2641 gcctggccat tgcttggtct gatcagttag tagccgtcat cgatggatgc gatcatatca
     2701 tcaagcctgt cggtgcctgg cagtatggca gtgtttctca tgcgtatcag gggctcatct
     2761 gagaactggt aggcccgggc attgcccgtc ggcaagactc ctaagtgcct ggcgctctct
     2821 ttgttgtgca tgtagccttt caagattccg ttctcgatta tcacggcgtc ttccgccttc
     2881 gttccttcgt catcgacgta tatgggcacc gggcatgtct cgccgccata cgaattggcg
     2941 aagtctatca gggttactag gggacttgca acttcctctc caaccttgcc tgacgccact
     3001 gaaccgctca tgacaatgtc ggcttcggtg gtatgcccta tggcctcatg ggccaggatg
     3061 cctgcaagct cggcgtcgag gacacatgtt ttcagccccg catcggggaa gactccctgg
     3121 ctctttctga ccaggtgttc atactgggtt tcgatgccat caaaaagctg cgaaggcgat
     3181 gtgaatacat cctcgaattg gccaaaaccg ccgtacacat cgctgagctc cgtcgggacg
     3241 ccatctttga cgagtgaaag gacgacatag atgaagcttc tgggaaccat ggaatgggag
     3301 tatgacccgt ccgatgtgag aagggtcttc tccatatcga ggcagttgat gccagtaatt
     3361 ctgctggcaa gctcggggta tttgagggct atatggttat ccagttccct tgcaaactcc
     3421 aggagctcca tttgcccaag cctgggcttt ctggtcgaat agtcatttac ggacgacgcg
     3481 gtggctacag gaagctgtcc tttgcccttg cgctctcttg aatcgagaaa gagggcattg
     3541 tcagtcgctg cctctattgc aagcttgagt gcgtcttcag ttatctcggg gttggacgcg
     3601 aatccccatg aaccgttact gtacactctt gccgatatac cgctggtaga tgtacgcgag
     3661 ttgtgagtgt tgttgcctcg gacgaaggta attcgcacgt ttcgattctc ttgtgctcgc
     3721 agttcagtat aggccgtaaa gagctcggca tattcgcgta gatcaagact atacaagcat
     3781 actcctcctt tgtgatctag gtttctgttc cgttgtggat gactgaggcg agatggcttg
     3841 aaactcaacc ctcgactgtc aattcatcca atggctctga atttcctctt tagggaagaa
     3901 aggcttgcat tccacatggc catttgagat accgccggat agaacggacc tgcatgtttt
     3961 tctgttggga catgtcagag ggtgcatgtt gtgaacaagg aagtgattac tgatgtgaga
     4021 cgccgtttcg gagactaagg agctggctac catgcctcta tcccatcgag atgtccccat
     4081 gcaagttcag tgtggatgtt cgaatcgcag gctttgggag cgtgatcgct tatgaagagg
     4141 ctcgttttgt gcgggcttgg cgaagtcatg atgggtatgg cttcgtgttc agcctggctg
     4201 tcgatggtcc ggtcgttcac acggtcatgg gattcgggca accccattcc agatagtaga
     4261 ctcatagctt gactggcggt ggggggaccg cacgatgaaa tcctgttcca gaaactgttc
     4321 gaggatgcca agaaacgcgg gcatctttcg ggatacaact gcgcgtgctt cgccaataac
     4381 gtagtctgga atcaatagag tgaaagctcc tgatagcgcc atgtccagga gccgtgcttc
     4441 tggaccgccc cagattatgc cagagaatat gatgttggta tctatcatga gtgtaagtct
     4501 actcatcgtc gtaatgatcc ctcatatact gagcggagag cccctctcgc acttcctcca
     4561 gtagctgttc cagttgctcg ggcgttatgc ctgaacgcct aaactcctct cctatggtca
     4621 gacgggcaac ctcaacgggg tccagcttct tgattatgat agctgaacca tctgcaacct
     4681 ggcacgagaa cctatcgcca gggtgaagag ctagtctctc cctgatgtgg ctgggaatgg
     4741 tcagttggcc cttggtactc atcgttgtgg tgctatcctt gcttggctgc acacctaccg
     4801 ccccttgcat tacgtaatac gctttacgca agcatggcat ttggctgact tcgatgcaac
     4861 tccggcagct ttcggacatc ttgaaacaga acgggcgcct gtcacttgaa tgtcactttg
     4921 ccttcctaac atagaattgg attctggcgg aatcgacgct tccgccctta gcttgctgct
     4981 ggaaacgtaa agtgtgatac atagagcgat tactgagagg ggagaaaata atatggaaat
     5041 actccgggct gagaatgttt caaaagtcta tcggggcgaa catgaggtac aggccttaga
     5101 tcaggtcaat ctcagcgttg aaaaaggcag cctgttggcg atcgtgggtg atagcggctc
     5161 cggcaagtct acgctgttac atattttagg cggcgttgat cgaccgacaa gcgggcgcgt
     5221 ttttgtacag ggaacagaca ttacagagct tcacgaaagc cagatgagcg tatttcgcag
     5281 acggaatatt ggcatcatat accagttctt caacctgatc cccaacatca atgtgcgcaa
     5341 gaacatatgt ctgcccttgc tcttggacga gcgagtgccg gatgaagatt attatcagga
     5401 agtcatccaa acgctcggca tcgccgataa gcaaaggcgc tttcccaatg agctttcagg
     5461 gggcgaacag caacgcgtcg ccatcgcgag gagccttatc aaccgtcctg ctgttatttt
     5521 ggctgacgaa cctactggga atcttgaccg ccgaaacacc aaagagattg tctccctctt
     5581 caaactcgtt aacaagagct tcaaagccac catcattctc ataacccatg acgagaaggt
     5641 cgctctcgcc tgcgagaaag tctgcaccat ggttgatggt cggctctatg agggggacgt
     5701 gagatgagca ttatcagaga actggcacgc gattctgtta agacagatag gaaggcttca
     5761 ttcgctgtta gcgtatctat tttcatcgct gttgtactgg tcggcttttt cctgatcatc
     5821 ttcagcctaa tcgactatgg tcaagtggaa tacatgacgg agaccacggg aggctactca
     5881 gccatgctcc ttgccataag tcctgaagac atggcagagc ttaaagcaga tcaccgagta
     5941 agatccctgg cggtaattga aagagaagaa ctgaaagatg caggtttgaa gcgtccccaa
     6001 aacgagatca attatcttga tgcccatcta tacgattaca aatgggttga gctggtatct
     6061 ggaaggtacc cggaaaatgc ccatgagctc ttggtttcag aactcttctt gatcgaaaac
     6121 ccgggttttg aaattggctc tgatgttacc attgcggatc gtcacttcta catctgcggc
     6181 acctttaagg aatacttgta ttcctttgaa ggcacgtata cgagttacgg ttggattgac
     6241 agcacgaaaa cacaagttat gggacctgtg gatctttgca tttggctcga gaatgaaagg
     6301 gctgcctatg agcacatccc cgacctggta cagaggctgg ggcgtgaccc cgcccaactc
     6361 aaaaaggatg gcggactcat ctacaataca gcttatctgg aaagcaagat gatttttccc
     6421 ggggggattc tcaaaccatc cagggatgtg gcggaaaaga tgttgcttcg cggcattgtt
     6481 cttctctgtt tcgtcgccct ctttgtcatg atcgtctata acgcctactc tgtttggaat
     6541 gatcgtgaca tgaaacagat cggcctattg aaaagctgcg gcatgagtga aagccaggtc
     6601 aaacgttatg tggtctgcaa agctttctac ctttcgctgt ggcctattct gtttggcatc
     6661 attgcctctt acctcttgac tcttttgctg tattacttgt ttgaaatgac acgggcgcaa
     6721 tatcacatcg ggtatactcc catagagctt cttaagaccc cttttgctcc tctagcgcga
     6781 gcagttttac caactctgtg tttggcgcta ttcactgttt accttgcatc actgaagcca
     6841 gctaagaaga gcagcaggct ctctgttgtg agcgcaatca agaacctgga ccccgagcga
     6901 aacctgaaac tgaaggacat acgctacaca tccaacatag agcgaagcct ggccaaggag
     6961 tacagcagca attatagcaa gacttatcgt ggcatgagta tcgctttggc gatggcctgc
     7021 ctgatttcct cggcggtgct agttgaacag agtttcaatc acctcgatga acgctataat
     7081 acctatgaga gtccatataa cctgaatgcg ttcctggtca cagtggacaa tgtcaatcct
     7141 gagatgatcg agcggctcag acaagtcgac gggatcagcc gtttggtcct ctatcgagat
     7201 aatgggagtc tcaagttcta cgagagcgat aatcctggtt tcctcagtga ggagaaaacc
     7261 tgtgcgcggg caaggctaga agaggataag aaagcattcg atgttcccat taacctgtat
     7321 ggcatcgatg atgcagagtg gaatcattac tgtgacgaac atgggcttaa atccagcggc
     7381 cgggacgcaa aaggaagcta ccaggttctc ctgctcgaca ggacttgcga agacccgggg
     7441 agaccctatt tgcggagcgt tcatattccc ttagccggtg aagccataag tgatattcat
     7501 ctgcgaagga aaactgatac cgaagcggga tcggtcagtc ttaagattgt ggacagactg
     7561 acggaagtgc ctttcggtat aggccctatg cctcggaaat ccatcagcct tttcatgcct
     7621 ttaagctgtt ttaactcctt ttgccaggat aacggtttcg atcagccgct ggagggcaaa
     7681 gcatatatga tctcaattac ggcgaatgaa gatatgctgg acgagataac cagggcgacg
     7741 aggcaggtta tgtatgaata cattcccaga agtgacgcaa gggtagaatc taggaatgat
     7801 atagatcgtc tgtcgcagga agaagcggcc aatagcaaga tcctcttcat tttcattcag
     7861 agctttctgc tccttgtcgg gctctccaat gcatataaca gcttcaactc caacttgcag
     7921 gccaggaagc gggattttgc cctcttgcgc agcatcggaa tggaagagaa gcaactgaaa
     7981 gggatgcttc tgcatgaggg aatcttcctg atacgaagag tgcttctgat tttcttgctc
     8041 ttgctcatgg ctttggtgac cttgtatgcc tatcgaaaga ggatgctttt tgcgccttgg
     8101 gagatcctgg ccaatctcaa tttcccgttg ttggttgcat ttctcgccat caacgcgatt
     8161 ggcatctttg ctgcgatcca aggtggaacg cgtcgcgtaa tgagccagaa catcctggaa
     8221 tcactgcgcg aagagtaaac gctcttcaca ggaagaggct ctatcaaaca gatggatcgg
     8281 ctccggcagc tgcaagctca tgataaagcc tgatttcaaa agagctgccg gagcttctgc
     8341 tttccacctc aaggctgccg ccgtgccctt caactataga tttggccatg gacaggccaa
     8401 tgccgaaact ctccttttgg gcgttttggc tcttaaagaa gcgatcgaag atgtgcctta
     8461 agtccgctgc cggaatgccg gggccattat cccgaactgt tatgctttgg tagatggtat
     8521 tctctgaaag ctggatctca acaagtccct tgtcatcaac ggcctccaca gcgtttttcg
     8581 cgaggttcaa gactgcttcc atgagccacg agcggtcccc tttgatctcg taatcctggc
     8641 cggtcagcct caagtcgacc tgcttttgtt gtatcaggca ttcaatcggt tgaattgctt
     8701 cgtagatcat gttctcagcg ctaaagattt cgctgcgaaa ggcaatagcg ccggcgtcaa
     8761 gtgaggagag cttcaacaag gcagaggtca gcctgtttag tcgatcaatc tcctcatcaa
     8821 gacgggcgat gtattccgcc tgattggctt cgtcctgctc aattaagtcc agcatgagct
     8881 gaattgtggt gaggggtgtc ttgatctgat gggtaatatc ctcaatgttt cttttgagaa
     8941 gtgcgcggtc agctaaggcg ttttctcggg attcgcgcag gatcaagatg gtcttgtaaa
     9001 tgtcatctcg cagctgtcca aagagatcat cgcgcaagac caatttgtct tcgccacggt
     9061 gtaaggccct aagataatcg gaaagctcga gtaactccct tttgagtttc ttctcttctc
     9121 tggcgctctg aaaagacacc aaaagtcctg cggcaagagc cagggccaga ttaatccatg
     9181 tgagcttgtg ctggtttata tagctagttt ggggcaaata gccatagttg cgcaagaatc
     9241 ttgtgccatc ggctatcagt tcatctggct tttcttttag ggcaattgct aaatctgtct
     9301 cggtcgcacc tggcgtatct tcggctgaat ggttgatcaa ggcagctccg gcaaagaggc
     9361 taagggcctt ttccccttct tgcagggcaa atcctgtggc caaagacaaa agcaaaaacg
     9421 agacgatgaa ggcaacggcg cccccggcaa cggtctgtct catctggaca tctctccttc
     9481 gatccagcga tagccgatgc ctcgaaccgt ctggatcttg ttctggtaat gccccagttt
     9541 gtgtcttagc cttcgaatgt taactgacag agtgttatcg tacacaaagt ctgactcaag
     9601 gccccaaacg atgtcgatga taagctctct ggttagcgtt cggcccggat tctccataaa
     9661 aaggcacagg agttgaaact cctttaagct cagctcttct tctcgctggt caataaagac
     9721 cttctttaga ttcttatcga gggttacgcc atcgctctgc aaaatgctct ggaactcggg
     9781 gtccttcttc acccgacgca gaactgtctc aattctcgcc ttcagcaccg gcaatttgaa
     9841 gggcttcgtg atatagtcat ctgctccgga gtccaatcca cggatgatat ccccgtcgct
     9901 gtccctaacg gttaagcaga taatggggat cgaactgttc tctctaacaa gatcaagaaa
     9961 agcaaagcca tctccatccg gcaagttcag atcgagaaga atcaagtcta gatcatcatg
    10021 aagagatgct ctcgcctccg acaacgtcga agctggaaaa accgcgtagc ctgcgttctc
    10081 taagtatcgc ttgactgcga agaggatatt ctcttcatcc tctaccagta ggattttctt
    10141 catgttgaaa gctcccatcc gtgaattagc ttggtgcgac cgcaacatta ccgagagcaa
    10201 ttgcatccgg cttgcctttt ctgtgtttgc cgcaatatgt gcatgtacat tataccgggt
    10261 tttgcccggt cgcaattgtg ttggtcatgc tgagtgtggc gaatgggacg gatgcctagt
    10321 ggccaacgat agatggcgac gcgcagtcct aggatgcgag ctttccacac cgggagggcg
    10381 ggcggagtgt gccggctagc agaagatgcg tgcaggatcc tcatgcccac gactcgtata
    10441 catacctgag cctatccgtc cgggttcgcc tctgtcagtg agccatctct gtatgcggct
    10501 ggaagggaag cttctccggg ggcatttccc gggccagatc ctgtcgggca gcatggttcc
    10561 aatgcttaac aaccaggaga atcagttcgt tcggcatgcg ctcgagtcga tcctttgcat
    10621 cctgatgtta tgcttctgac tcaatcaggc atgtgggcat ttgatgccaa gtttcgcttg
    10681 gacagaggtt ctgtttcggg ccatgctacc aaagcggata cgttgggtgc gatggaggat
    10741 acagccaggg ttgatgacat acacaaaatg cacacgtacc gtgatgcgct cacccctggt
    10801 ttgagagggg cttatgtggt gtatccaggc gatgaggtgc tgatgtaccc tgcaccgggg
    10861 tctagtgcat gtaatgaccg accaggtttt agggacgggg gtccctgggt tgccgtagat
    10921 ggggtaggag cagtgccggc gaggccgggc tcgggcatga ccgggctgtt tggagtgcta
    10981 gacaagatat tgagccttcg gtaagactca cactagagag gcagatgaaa gagatcgtgg
    11041 aggagaagag gaaacgccta ggcatagtcc tttgtgccat agctatagtc atgactgtat
    11101 ttggactcat agagaccaac agaagtcgga agatagtaca gattctcgac aactaccaca
    11161 gagttgaccg aatagccatt aacagaggcg atgtggaggc ggtcgtggcc ggggatgagc
    11221 tggagcgata caaggaagcc tttgaacccc aagtcatcct gtcgggggat aggaaaacga
    11281 tcaacagggt tttgggggat aagtatctag agatgagatt ctttatcgag gatgaactcc
    11341 tgtttagcgc ggccatttac gaattgacta caggcagtaa tgttccggac agttttgaaa
    11401 tgggaagccg gagatacatc atgaaggtcg gtcgagacta cagaagcctg a
//
LOCUS       DAOSYL010000067        10192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5603_length_10192_cov_8.298017, whole genome shotgun sequence.
ACCESSION   DAOSYL010000067 DAOSYL010000000
VERSION     DAOSYL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5603_length_10192_cov_8.298017"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(427..1335)
                     /locus_tag="PLU15_09240"
     CDS             complement(427..1335)
                     /locus_tag="PLU15_09240"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOO31141.1"
                     /translation="MRRISRSVQRRMATVLMAALLAVAIAGQAFAAEETSDPVGTAAM
                     LNLGMGARAIGMGGAHIAVADDATAIYYNPAALDVIRDRNVTSLYARLHGAADYLALG
                     YAQERVGVGVYGLSASGIEETDEYGNVIGTFGVNDFVLAAGYGREVASGLSLGATVKV
                     YSESAPGNAGKGITGDVGVLYSMNDGKLRLGAVGRNLVGGVRYESGATDDFERSFGAG
                     VAYLPMDNLLIAADAVFEDGFAGCAGVEYRVGKIALRAGGNYGAGGSSLTAGAGFAVA
                     NFTIDYAYQSHSVLPDSHRLSLGVRF"
     gene            complement(1494..6980)
                     /locus_tag="PLU15_09245"
     CDS             complement(1494..6980)
                     /locus_tag="PLU15_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO31142.1"
                     /translation="MKRHTRWLLVVTVLVAVAALGIGWSAMANERSAVALDPEQWAPA
                     WQEGAEEFVPGEYLVRFVPGVQTVEAENIMGRSTMSIIKEIKFDPCKAFPEGLTLYHV
                     RVEPGVVSAEAVCALEADPRVEYVEPNRKIFAAETVIPDDEYFNLLWGLHNIGQTFIG
                     NVSGVEDADIDAPEAWAHRREAPEVIVAVLDADIQVDHPDLAPRMFINEGEIPGNGID
                     DDGNGFIDDVCGWDFHHNDNTLYDGESHHGTHTCGTIGAVGNNGIGVVGVAWNVKILP
                     IKFIGPSGGSLADAAAALAYARQMGATITSNSWGYSGGPDKALEDAFAASDMLAICAA
                     GNVAMDNDSNPNKSHYPSSYPLDNIIAVAATDCNDELAYFSCIGATTVDLAAPGHRIL
                     STVPEEMYEVPYAFLSGTSMATPHVAGAAALLIAEYGDMPQYPGAEGYVEGDMTIKDL
                     LLATVDKKPQLYGKMVSGGRLNLRNALLQMMPPVIESVGVDYTYGTPPLTVNFTAKVG
                     APESIADMWWDFADGSELCYEPNTTHVYENAGSYKATFHAVDVNGLEATASVAVAVFD
                     DSYFVMIDDDGGNAVDEWYTNSLDRLGYKWVKLEPPVTLPTDITNPVIWFTANAARTT
                     LTAEDQAWIGEYLDNGGRLFLSSQGVIDDLDIDHPFIHEYLHVASVRKNVGAKRVFGV
                     PGDPMTDGWDITTEPLFMDRSDSIIPDDVAVPILINDSGRACALRYVGDNGSRLIFFS
                     FPFEFIPGGATDSGPGSGSEDRPAVKVMDAIAKFLTEPGVAQVEVSPSSLEAVMAHGT
                     TKQETLTISNKGDCDLYFDLSVAMPVQAEKNGGPDEFGYVWQDSDDPNGPAVEWVDIT
                     EKGTLAVFGDDYYKELDLPFEFDFYGQKKNSILISTNGYVTFGLRGAASSHTPIPDAT
                     APNDMIALFWADVNARDGGVYYWHDARNGRFIVSYDGVQKQNEEYEPEGSFDFQVHLY
                     EDGRIVLQYGELDGVLDGCTVGIENSSGTVGLQVAHNELYLHSNMAIEFTRSSDALSF
                     KPARGVVRPGESAAIAVELDARALPEGLHKRDIVVATNDLNSPSLAVPVSIEVTPNAV
                     PVIVRAEATPSQGEPPLRVTFAAEAIDPDANGAVADMWWDFGDGSEPEHVAETSHVYA
                     EAGRYMASFYAVDDMGAEASVSLWINVARAPHAAIDVDSVDVAVLPDSQGVAYVKIQN
                     TGDVPMRYRVTGLEKPGDVKITKTAGTDELDPTSPYAISTFEVEGDSVRTLKPEAVLK
                     SWEIPAASEGGPGWPWGCGFDGEHIWVSDVKKANFKYTTDGEFTGDRFDTTQWTTGWG
                     GDFAWDGNLMWQVSVGGKNYIGGLDPQTGECLDIISDPDGIWDGHAERGLAYDPVEDV
                     FYIGGWVDYKIYKIAGKSWPEPGKILRVYDDVTLAVAGIARVGWHLVLTLNTHPDLVA
                     VMDSITGEIITWMESPSVSSIRVGGCEQDENGNVWMVYQGEQERPGMAYLLDMGLALN
                     DPPEWMSISEGEGILQPGEAAEVEVKFNASSAMKPGDEYEAQIVVTTSDPENQMLVLP
                     VNMRINAAPAADFSFARTGEVAVRMIDESTDSDGDIAAWSWSFGDGTTSTEKNPVHEY
                     AEIGTYEVTLTVADNDGATDTITKVVDIDALPSVVVLAPFGGEVWMGTNEIRWTASDT
                     DTLPDELLIKLEFSSDGGKTWKPIMDGVENNGSYTWNTAVVEKGDRYVVRVTATDPEG
                     GIGEGESGEFTIIILSEAVVAAPNPANQNVTFYYNIAADGKLYVYDIAGRLVYTADMA
                     SAANAHDWNLVATDGRPIANGLYLYVIVTEGGEVSKVGRLVVAK"
     gene            complement(7583..8308)
                     /locus_tag="PLU15_09250"
     CDS             complement(7583..8308)
                     /locus_tag="PLU15_09250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO31143.1"
                     /translation="MIKTVMFDLDGTLAHFEFEEFFRAYIEKIVESLSDVMEPKALTE
                     ALMASVEAMVSCEDPALTNRDVFVVDFFGRTGRSEAELMPLFDAFYLAEDGFPSIKQM
                     LGVTPHPSARGMIENLLDRGYEVVIATNPLFPLVAIEERMRWGDVDGLPYALVTSYET
                     SHFCKPNLKYYEEILSVLDRSPEECLMVGNDTWEDLVAGDLGITTYLVEDYILDRDPV
                     RRDPDFRGSFDDLVEFLSAEEFL"
     gene            complement(8424..9452)
                     /locus_tag="PLU15_09255"
     CDS             complement(8424..9452)
                     /locus_tag="PLU15_09255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO31144.1"
                     /translation="MTAKEIKLVAAIMLTLLAFSAGVGAEEAQTTFLVVTLKDGSSVV
                     YNIPVDGDKIDSAALAFSDVGLHELEGKHNLVALINNEPITTGAFYDEISNVVGYQAL
                     SYIIDETLVRQAAKSAGITVTQAEVDREFEMVREATGSDFDAVLAQYGMTQESFRKSL
                     EMSLLVFKVSTKDVKVSAEDIAEHYNSHIADYTIPEQVRASHILVGAEKEAEEILQLL
                     ESGADFGELAKERSEDTYSAKAGGDLGYFPRGAMVPEFERAAFSTPVGETSGIVKSQY
                     GYHILQVTGKMDARTIPMEDVWESIEWAIKSKYALDFKQLVAELKRDSAIIVFDPRFA
                     DLGTTTLP"
     gene            complement(9449..>10192)
                     /locus_tag="PLU15_09260"
     CDS             complement(9449..>10192)
                     /locus_tag="PLU15_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31145.1"
                     /translation="FDWDPEVINRKVAAEIEALSELADAQLDSELHLQLRRIANVNSE
                     ADCDEIAEGVILRAARSLGISAEEYGSLEDVEAAVFVACVAKQIDLLLELVGEMQADE
                     AESAAAKLGEELESLAPDEIAAVCEAIGIESISEEAVAAGLRFCRAVPMAQMVCDDFG
                     YASTKFLVSLGKAVGFALGCAFLPGVIGGVDRFMACALSPFIAVLSGRAALATSDRML
                     YDELARFLIVIGHGVVIEDRRNDWRQALS"
BASE COUNT         2337 a         3177 c         2382 g         2296 t
ORIGIN      
        1 cacaaaacca tagatactct gcccctcgct gacgaggaat gctctgggct agccgcgatc
       61 tccatatgtc aggcgggtag tacatgagac atgctagaaa cgtggtgcct cccagtttga
      121 gaccgctgga acggttagcg caaaccaggc caaatcgact atcactacca tgaggctcat
      181 cacagtgccg gtctggatcc tgaaacaagg caacatctcg atctcacggc caaccgcaac
      241 caggtagtgc ggtctcttcc gactagcata gatctcagtg tcgcttactc gatagaaaac
      301 aatgcgttat ttgaagaccc ctctcacaca gagaggggtc ctcactatag ttgctttgcc
      361 cttcagaaac agggcccagt tcgccagccc gcctgaagtg cagtctatga ctcggtcgca
      421 gctctactag aatcgtacgc ccagcgaaag cctgtgcgag tcaggcagaa cgctgtgaga
      481 ctgataggca taatcgatcg tgaagttcgc aacagcaaac ccggctccgg ctgtaagcga
      541 agacccgccg gccccgtagt tgccgcctgc ccgaagagcg atcttgccta ctcgatactc
      601 aacgcccgca cagcccgcaa agccatcctc gaacacggca tcagcggcta tgaggaggtt
      661 atccatgggg aggtacgcaa cacctgcgcc aaaggacctc tcgaaatcat cggtggcgcc
      721 gctttcgtat ctgacaccgc ctacaaggtt ccttcctacc gcgcccaagc gaagcttccc
      781 atcgttcatg gagtagagaa caccaacgtc cccggttatg cccttgccgg cattgcccgg
      841 cgccgactcg gagtagacct tgaccgtcgc tcccaggctc aggcccgaag ctacctcccg
      901 accatatccg gccgccagca cgaaatcgtt cacgccgaag gttccgatta cgttgccata
      961 ctcgtccgtc tcttcgatcc ccgaagcgct cagcccgtaa acccccacgc ccactctttc
     1021 ctgcgcatat ccaagagcca agtagtctgc tgccccatgc agccttgcgt atagcgaagt
     1081 cacattgcga tcacggatga catctaatgc cgcagggtta tagtatatcg ccgttgcatc
     1141 gtcggctacc gcaatatggg cgccacccat gccaattgct cgcgccccca taccgaggtt
     1201 tagcatggca gctgttccaa ctggatcact cgtttcctct gcagcaaacg cctgtcccgc
     1261 aatggccaca gcaagaagcg ccgccataag aacagtcgcc atgcggcgct gcactgacct
     1321 gctgatccgt cgcataattt gctccgtcct ttcgtatcca catcagtggc ctcatcttgt
     1381 aatctacgtc gtctgccgcg ggctgcgcct gcaggcgcaa cccgcgaccc ataaagcttc
     1441 aagatgcggg gccggatcgg cagacaccgc ccgcccccgc atattcctgt atgctacttt
     1501 gcaaccacaa gccttccgac cttggagacc tctccgccct ctgtgacgat gacgtagaga
     1561 tagagaccgt tggctatagg tcgtccatct gttgcaacga gattccagtc atgtgcattc
     1621 gctgcagacg ccatatcagc cgtgtagaca agtcttccgg ctatgtcata cacatacagc
     1681 ttgccatctg ccgcgatgtt gtagtagaag gtgacgttct ggtttgctgg gttcggagct
     1741 gccacaaccg cctcagacag gatgatgatc gtgaactctc cgctctcgcc ttcaccaatc
     1801 ccgccttcgg gatccgttgc agtcacccga accacgtaac gatcgccctt ctcgaccaca
     1861 gcagtattcc aggtgtaaga cccgttgttt tcgacgccgt ccataatcgg cttccacgtc
     1921 tttcccccgt ctgatgagaa ctcgagcttg atgagtagct catcagggag cgtgtcagtg
     1981 tctgacgcag tccatctgat ctcattggtt cccatccaga cctcaccgcc aaacggcgcc
     2041 aacacgacta ctgacggaag cgcatctatg tcgactacct ttgtgatggt atcagttgcg
     2101 ccatcattgt cagcgaccgt cagggtcact tcgtatgtgc caatctcagc gtattcatga
     2161 actggattct tctcagtgga agtggtgcca tccccgaacg accagctcca cgcggctatg
     2221 tcgccgtccg aatcggttga ttcatcaatc atcctcacag caacctcgcc agtgcgcgcg
     2281 aaactgaagt ccgccgcagg cgcggcgttt atccgcatgt tgaccggcaa gaccaacatc
     2341 tgattttcag gatcgctcgt cgtcaccact atctgggcct catactcatc tccaggcttc
     2401 atcgcgctag atgcgttgaa cttcacttcg acctctgcag cctcgccagg ctggagaatc
     2461 ccttcgcctt ccgaaatact catccactca ggcggatcgt tcaaggcaag gcccatgtcg
     2521 agcaggtatg ccataccagg acgttcctgt tcaccctggt ataccatcca tacgttgccg
     2581 ttttcgtcct gttcacaacc gccgacccgg atcgagctga ccgatggaga ctccatccag
     2641 gtgatgatct cgccagttat gctgtccatc acagcaacaa ggtcgggatg ggtattgagc
     2701 gtgagcacca ggtgccagcc gaccctagcg atacccgcaa ctgcaagcgt cacatcgtca
     2761 tacactcgca ggatcttgcc aggctcgggc caggacttac ctgctatctt gtatatcttg
     2821 tagtccaccc agccgccgat atagaatacg tcctcgacag gatcataggc caatcctctt
     2881 tcagcatggc catcccagat tccatccggg tcagatatga tgtcgaggca ttcacctgtc
     2941 tgaggatcga gtcccccgat gtagttcttg ccaccgacac tcacttgcca catgagattt
     3001 ccgtcccagg cgaagtcgcc tccccaccct gttgtccatt gggtggtatc gaatctgtct
     3061 cctgtgaact ccccgtcagt ggtgtacttg aagttggctt tcttcacgtc tgacacccag
     3121 atgtgctctc cgtcgaagcc gcaaccccat ggccatcccg gtccgccttc actggccgcg
     3181 ggaatctccc atgacttcaa aaccgcctcc ggcttcaacg tgcgcactga atcgccttcc
     3241 acctcgaatg tggagatggc gtaaggtgaa gtcggatcga gttcatccgt tccggcagtc
     3301 ttcgttatct tcacatctcc cggtttctca agcccggtca ctcgatacct cattggcaca
     3361 tccccggtgt tctggatctt cacgtaagca acaccctggg aatcgggcag cactgcaaca
     3421 tccaccgagt ccacatctat cgcagcgtgc ggcgcccgag ccacgttgat ccacagtgaa
     3481 acggatgcct ctgcccccat atcgtccaca gcataaaatg atgccatgta gcgtccggcc
     3541 tcggcatata cgtgactcgt ctcggcaaca tgctcaggct cggatccatc gccaaagtcc
     3601 caccacatgt cagccacggc gccgtttgca tccggatcaa tagcctcagc cgcaaacgtc
     3661 actctaagcg ggggttcgcc ttgcgatggg gttgcttcag cgcggactat cacaggaacc
     3721 gcgttgggcg ttacctctat cgaaaccggc actgcaagag acgggctgtt gagatcattg
     3781 gtcgcaacca caatgtccct cttgtgcaat ccctctggca gcgctctcgc atccagttca
     3841 acagctatcg ctgcactttc gccaggcctc accacgccac gcgcaggttt gaagcttaag
     3901 gcgtcacttg aacgcgtaaa ctcgatagcc atattgctgt gcaagtaaag ctcgttgtga
     3961 gcaacctgca gtccgacggt tccagaagaa ttctcgatgc ccacagtaca cccatcgagg
     4021 actccatcga gctcgccata ttggagcacg atccgaccgt cctcgtacag atggacttgg
     4081 aagtcgaacg aaccctcagg ctcatactcc tcattctgct tctgcacgcc gtcatatgag
     4141 actatgaagc ggccgttgcg agcatcgtgc cagtagtaga ctccgccgtc cctggcatta
     4201 acgtcagccc agaacagagc gatcatatcg tttggagcag tagcatccgg tatgggcgta
     4261 tggctggagg cggcgcctct taagccaaat gtgacgtatc cattggtgct tatgagaatg
     4321 ctattcttct tctgcccgta gaagtcgaat tcgaacggaa gatcgagttc cttgtagtag
     4381 tcatcgccga atacagccaa cgtgcctttt tcggttatgt ctacccattc gactgcaggc
     4441 ccattggggt catcgctgtc ttgccaaacg tatccgaatt catcgggtcc tccgttcttc
     4501 tctgcctgaa caggcatggc aacagataga tcgaagtaga gatcgcaatc gcccttgttg
     4561 gatattgtaa gcgtctcttg cttcgtcgtg ccatgagcca tcacagcttc cagcgaggaa
     4621 ggtgaaacct ccacctgcgc aacgccgggc tccgtcagga acttggcgat agcatccatc
     4681 actttcactg caggcctgtc ctcagaaccg gaacccggcc cgctgtcagt tgctcctccc
     4741 gggatgaact caaacgggaa ggagaagaat atcagcctcg aaccattatc tccgacatat
     4801 ctcagagcgc atgcccgtcc cgaatcgttg ataagtatcg gaacagcaac atcgtctgga
     4861 ataattgagt cgctcctgtc cataaacaga ggctcagttg ttatgtccca cccatcagtc
     4921 attggatccc caggaacacc gaatacgcgc ttggctccga cattcttcct aacactcgcc
     4981 acgtggagat actcatgaat gaacggatgg tcgatgtcca ggtcatcgat tacaccctgg
     5041 ctcgagagga acagcctccc gccgttatcc agatactcac cgatccacgc ctgatcttcg
     5101 gcggtaaggg tggtcctggc tgcattagcc gtgaaccaga taacagggtt tgtgatgtca
     5161 gtcggcaacg tgacaggcgg ctcaagtttc acccacttgt aacccagcct gtcaagggag
     5221 ttggtatacc actcgtcaac agcgtttccg ccgtcgtcat ctatcataac gaaataagaa
     5281 tcgtcgaaaa cagccactgc tacagaggca gtagcctcca gcccatttac gtccactgcg
     5341 tggaatgtcg ccttgtagct gcctgcgttc tcgtacacgt gagttgtatt gggctcataa
     5401 cacagctcac tgccgtccgc aaagtcccac cacatgtccg caatactctc cggagcaccg
     5461 acctttgcgg tgaagttgac cgtaagcggc ggggtgccgt atgtatagtc cacacccact
     5521 gactcaatta ccggaggcat catctgaagc agcgcattgc gcagattcag cctgccgcca
     5581 gaaaccatct tgccatagag ctgcggtttt ttgtcaacag ttgcaagaag gagatccttg
     5641 atagtcatat ctccctcaac atacccctca gctccagggt attgtggcat gtctccatac
     5701 tccgcaatca agagcgcagc agcacctgca acatgcggag tcgccatcga agtgccgctg
     5761 aggaatgcat atggcacctc atacatttcc tccgggacag tggacaggat cctatgccca
     5821 ggagcggcga ggtctacagt cgtagcgcct atgcaggaga agtatgcgag ctcgtcattg
     5881 cagtccgtgg ctgccaccgc aatgatgttg tccagcgggt agctggaggg ataatggctc
     5941 ttgtttgggt tgctgtcatt gtccattgca acgttcccag ccgcgcatat cgcaagcatg
     6001 tcagacgcgg caaaagcgtc ctcaagagcc ttgtccggac cgcccgagta tccccacgag
     6061 ttgctcgtta ttgtggcgcc catctgcctg gcataagcga gcgcagctgc agcgtcagcc
     6121 aagctgccgc cgctagggcc tatgaacttg ataggcagaa tcttcacatt ccatgccaca
     6181 ccaacaacgc ctattccgtt gttccccaca gccccgatag taccgcatgt atgtgttccg
     6241 tggtgagatt ccccatcata caacgtgttg tcgttgtggt ggaagtccca gccacatacg
     6301 tcgtcaatga acccattgcc atcgtcatcg atgccgtttc ccggaatctc tccttcgttg
     6361 atgaacatgc gaggagcaag gtcgggatgg tccacctgta tgtcggcatc caagactgcg
     6421 actattacct ccggcgcttc ccgccggtgc gcccaggcct caggcgcgtc tatgtctgca
     6481 tcttcaaccc cggacacatt tccgataaag gtttgtccga tattatgaag gccccacaga
     6541 agattgaagt attcatcatc aggaatcaca gtctcagcag caaatatctt tctgttaggc
     6601 tccacatatt caaccctagg atctgcttcc agcgcacata ccgcctccgc agataccaca
     6661 ccaggctcta ctcttacgtg atagagggta agcccttccg ggaatgcttt gcagggatca
     6721 aatttgatct ccttgataat actcatagtc gatctgccca tgatgttctc tgcttctaca
     6781 gtttgcaccc cgggaacaaa tctcaccaag tattcgcctg gcacgaactc ctccgccccc
     6841 tcttgccacg caggggccca ttgctcaggg tccagcgcga ccgcggaacg ttcgtttgcc
     6901 atggctgacc agccaatgcc aagggctgcc actgcaacca acacagtgac gactaacagc
     6961 catcgagtat gtcgcttcat tgcaaaattg catcctcctc tatttcgatg tcacatacga
     7021 taaaaaatac gtctacccag caaccacaga tcagcatcta ttccccatgt tttgcaggaa
     7081 agcatcactc cctccaatcc gttcaactct cattgagttt tcactcaaca aacgggcgtt
     7141 ccatgaatct gtctgcttga taatataaag aacaggtcac tcgccacagt gccctcagcg
     7201 gccctctgga agtgacccgc gatacgtgcc tgaatggccc gcatggcgtc tagatctcgt
     7261 tgatgccttg tcacacataa aaaactctgt tgcaagacgc caggctcaaa aaagcagagc
     7321 ccttttgctc aagaagatgc gttcgtttgg ataccaagct ggacggccaa tccaggcaag
     7381 ctggataacg cggtcagcgc aactgtcttc gctgctactg atcttgccac taccaatgcc
     7441 acaaaccaag cgcacattca ctagacagac tccggtctca ctaatgtttc tacctggggc
     7501 ggcgaattcc ttccatttgt gcatggattg gtaaaactgc caggccgttt aggcaattat
     7561 ggcgccccga gcaacacatg cactacagga actcctccgc cgagaggaac tccacaagat
     7621 cgtcaaaact gcccctgaag tcaggatctc gccttacggg atcacgatct aagatgtagt
     7681 cctcaaccag gtaagtcgtg atcccaagat ctcctgccac aagatcctcc caggtatcat
     7741 tccccaccat caagcattct tcaggggatc tgtccaaaac agagagtatc tcttcgtaat
     7801 atttcagatt aggtttgcag aaatgtgaag tctcatacga tgtcacaaga gcgtaaggaa
     7861 gaccgtccac gtcgccccaa cgcatcctct cttcaatggc taccagagga aagagcggat
     7921 ttgtagcgat cacaacttcg tatcccctgt caagcaggtt ttctatcatg cctctggcag
     7981 aagggtgcgg cgtgacgcct agcatctgct taatgctggg aaacccgtct tcagcaaggt
     8041 aaaaagcatc gaacagcggc atcagctcgg cctccgatcg ccctgtcctg ccgaaaaagt
     8101 cgaccacaaa gacatcccga tttgtaaggg cgggatcttc acacgagacc atcgcctcga
     8161 ccgatgccat cagagcctct gtaagagcct tcggctccat aacatctgac aggctctcta
     8221 cgatcttctc tatatacgcc ctgaaaaact cctcgaactc gaaatgtgca agagtgccat
     8281 cgaggtcgaa cataacagtc ttaatcatgt aatcacctcg gatatgaaag ctagtgtacg
     8341 gcctccggca catggctaga cgattcgccc tatgcagccc cagccttgcc aaggccacgg
     8401 cagcacccaa ctgagtaaaa tgtctatggc aacgtcgtcg ttccaagatc tgcgaaacgg
     8461 ggatcgaaca cgattatggc gctgtcacgc ttcagctctg caacgagctg cttgaaatcg
     8521 agagcgtatt tgctcttgat cgcccactca atgctctccc aaacatcctc catgggaatg
     8581 gttctggcat ccatcttccc agtcacctgg agaatatggt atccatactg tgacttcaca
     8641 attccgcttg tctccccgac cggagtcgag aatgcggccc gttcaaactc gggtaccatg
     8701 gccccccggg ggaaatagcc caggtcaccg ccggccttcg ccgaatacgt gtcctcagac
     8761 ctttccttgg caagttcccc gaagtccgcc cccgactcga gcaactgcag gatctcttct
     8821 gcttccttct ccgcgcctac aaggatgtgg ctcgccctca cttgctccgg aatcgtgtag
     8881 tccgcaatgt gagagttgta atgttcggca atatcctctg ccgacacctt cacatccttc
     8941 gtagagacct tgaataccag aagactcatc tctagactct tgcgaaaact ctcttgcgtc
     9001 atgccgtatt gggctagaac agcgtcgaag tcagaacccg tcgcctcgcg caccatctcg
     9061 aactcacggt ctacctcggc ctgtgtcacc gtaatccccg cgctcttggc tgcctgcctg
     9121 acaagggtct catcaatgat gtaggaaagc gcctgatagc ccacgacgtt cgatatctca
     9181 tcataaaaag cgcctgtcgt gatcggctca ttattgatca aggcaacaag attgtgcttc
     9241 ccctcgagct catgaagccc gacatcggaa aaagcgagtg ccgccgagtc tatcttgtcg
     9301 ccgtcaacag gtatgttgta gaccacagat gagccatcct tcagcgtcac cacaagaaag
     9361 gtcgtttgag cttcctcagc gccaaccccg gctgaaaaag caagaagtgt aagcataatc
     9421 gccgcgacca gtttaatttc ctttgctgtc atgataatgc ctgcctccaa tcgttccttc
     9481 ggtcctctat aaccacaccg tgtcctataa caatcaaaaa ccgagccaac tcgtcgtaga
     9541 gcattcgatc ggacgtggct agcgctgccc tgcccgagag aacggctata aacggcgaca
     9601 acgcgcacgc catgaaccta tcgactccgc caattacccc cggcaagaac gcgcatccca
     9661 gagcaaaccc aaccgccttg ccgaggctca caaggaactt cgtagatgcg taaccgaaat
     9721 catcgcacac catctgcgcc attggcacag cccggcagaa cctgaggccc gccgcaaccg
     9781 cctcctcaga aatggactcg atccctattg cctcacacac cgccgcgatt tcatccggcg
     9841 ccagactttc cagttcctcg ccgagcttgg ctgctgctga ttccgcctca tctgcctgca
     9901 tctcgccaac tagttcaagc aggagatcta tctgcttcgc cacgcaggcg acaaataccg
     9961 cggcctccac atcctccaaa ctcccatact cctcggcact aatccccagg ctacgtgctg
    10021 ccctcagtat gacaccttcc gctatttcat cgcaatcagc ctcgctattc acgttggcta
    10081 tgcgccgcaa ttggagatga agctccgagt caagctgtgc atcggcaagc tccgacagcg
    10141 cctcaatctc cgccgcaacc ttgcggttga ttacctcagg atcccaatcg aa
//
LOCUS       DAOSYL010000068        10144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5636_length_10144_cov_8.825255, whole genome shotgun sequence.
ACCESSION   DAOSYL010000068 DAOSYL010000000
VERSION     DAOSYL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5636_length_10144_cov_8.825255"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PLU15_09265"
     CDS             <1..128
                     /locus_tag="PLU15_09265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO31146.1"
                     /translation="TVSVGVASYPEAGSTVDELVRAVDAAMYKAKRQGKNRVVCV"
     gene            complement(161..1069)
                     /locus_tag="PLU15_09270"
     CDS             complement(161..1069)
                     /locus_tag="PLU15_09270"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOO31147.1"
                     /translation="MERTPVAIVGYGKVGRAAADAIAASPDFELTGIVRRKVVSDELI
                     RGRNWAAPAVTDISHLGGVQAALMCVPTRELERMEHGLLRSGISVVDAFDIHGDAICQ
                     HKEALHSSAIAGRAVAVIASGWDPGIDSLIRALMELMAPRGITYTNFGPGMSMGHSVV
                     ARSVAGVRDAISITLPAGYGKHRRTVYVEEDGSRPFQEIVRDITRDPYFSDDDTEVFS
                     VASVAPLADSAHAVEISRKGTAGEASNQLLEWQMKISNPAVTAQAMLGGLRAALKSEP
                     GAYTLLELPVAQMLPGNPRDNIKRLT"
     gene            1209..3830
                     /locus_tag="PLU15_09275"
     CDS             1209..3830
                     /locus_tag="PLU15_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOO31148.1"
                     /translation="MQVIVERAERCRRCHVCVTACPVGAIVISSGEVRLRRDLCIACG
                     QCVKACPNGALAIRDQGEDVMALIGNGRTVAILDPSFPGAFPQSDPLCLIGALRELGF
                     SSIWEASFGAESLLPYYADYVKAHSEESGRSVAFASMCPAVVAFIEHCRPELAGNLVP
                     LVSPTVAASRVARALGEKRVVFIGPCPAEKLEAGFGGVPGGPNIAITYAELSRLLDNV
                     GVSVDQCPASLPDVRASGAGRMLGLPAGLLRALRIRDDVAPPGILLAEGKEECLKLVE
                     ASLSGALGGSFVVASICRSCVDGTGFPEGASLWDRRRAVVDYIKEASSVGSMEVDGAL
                     RAVSASADFKRRYLPRKIPLAPKETDVRTALLGMGMRSPEDELDCGLCGFQSCRERAI
                     AVARGTTEASECFPRRNARLSEECARLSQLASDLDAIVNTSCEISYVCDPDGWIVRAS
                     RAANDIIGVENANLHKVNMKNLPRRLCPDVAIQALHEVKRVMAIRDLPGGHRLLAAAT
                     PILDGDGACRGVAATITDLSGAALEINTTPTANSFATDADSEDMVAYSPAMEELLTLV
                     HKVANVSSTVLITGESGVGKEVVARYIHRTGNRASGPFVKINCAAIPHTLLESELFGY
                     EEGAFTGARKSGKPGLIQLASGGTLFLDEISEMPLGIQAKLLQVLQEKCLVRVGGTEQ
                     VRVDARIMAATNRDLRAAVKEGRFRGDLYFRLNVIPIRVPPLRERSGDIAPLTYHFIE
                     KWDSRYGRNHQISREACSLLECYRWPGNVRELENLIEFLVVTVDDPVILPEHLPQHVV
                     AGEGERDVQVSVSGIMPLRWAVEEVERQLIALAAEACSTTYSMAKALGVNQSTVVRKM
                     QKYMETPEEEAEETGNV"
     gene            4105..4599
                     /locus_tag="PLU15_09280"
     CDS             4105..4599
                     /locus_tag="PLU15_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOO31149.1"
                     /translation="MGCCESKCVGDITPEQWAQVDGIIAKYKGQRGILIPCLHEIQEA
                     VGYLPLEVQERVAAGLNVPLADVYSVVTFYALFSTKPKGKHQVGVCLGTACYVRGGDK
                     ILEKVEQALGIKAGDTTDDRMFSIQVMRCIGACGLGPVMTVDDDIYARIKPDRVAEVL
                     AKYS"
     gene            4638..7685
                     /gene="nuoF"
                     /locus_tag="PLU15_09285"
     CDS             4638..7685
                     /gene="nuoF"
                     /locus_tag="PLU15_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOO31150.1"
                     /translation="MDLVRAHVLVCGGAACVSSGCAEVREALEAEIAAKGIGREIKVV
                     VTGCMGPCDLGPIIVVYPEGVLYCNCTPDDAREIVDEHLVKGRIVSRLLYEEPMTSEK
                     VETQEGMTFFSRQERIALRNTGVIDPLEIDEYIARDGYAALGKVLTEMTPDDVVDVIK
                     RSGLRGRGGAGFPTGTKWLLTAKAPGSPKYVVCNADEGDPGAFMDRSVLEGDPHAVLE
                     AMTIAGYAVGANQGYIYVRAEYPLAVEHLQHAIGQAREYGLLGKDILGSGFDFDIDLR
                     VGAGAFVCGEETALLASIESRRGEPRPRPPYPAQSGLWGQPTLINNVETYANIAPIIL
                     KGPEWFSSIGTEKSKGTKVFALAGKINNTGLVEVPMGTPLGDIIFDIGGGIPNGKKFK
                     AAQTGGPSGGCIPAQYLNTPIDYESLKELGTMMGSGGLIVMDEDTCMVDLAKFFMQFI
                     QDESCGKCAPCRIGTKRMLEILDRITKGQGREGDVELLMELGEQIMATAMCGLGQSAP
                     NPVLSTIRYFRDEYDAHIKDHKCPASSCAALFYSPCQNSCPADVDVPRYIALIKERKF
                     AEAAALIRSKNPFPAICGRVCDHPCESKCRRATFDEPVAIKSLKRFAADWEKKHGVSP
                     VAEHKPPTGKRVAIVGAGPAGLSAAYYLALAGHKVTVFEALPVAGGMLAVGIPEYRLP
                     KDILEWEIEVIKKAGVEIRTNTRIGEDIKLDELSSEYDALFIAVGAHNEVKMDVPGED
                     FEGVMPAVAFLRDVALGKPVEVGKKVAVVGGGNAAIDAARTARRMGAEEVHILYRRLR
                     ADMPADAGEIEAAEEEGIHIHYLTAPTRIIGRDGKVAQIECVRMKLGEFDKSGRRRPI
                     PVDASRTIVDIDTVIPAIGQAVDTSFIMPLSDTGENLFTTKSGLLITDPDSLETNVPG
                     IFAGGDCVTGPWSVVAAIGAGREAAYSIDRFLGGDAWIGAHAEIERCLVGEPIEEECK
                     RCPMPELPVSERARNFKEVELGFTEDMAVAEAARCFQCDVREP"
     gene            7712..9448
                     /locus_tag="PLU15_09290"
     CDS             7712..9448
                     /locus_tag="PLU15_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOO31151.1"
                     /translation="MGDVTLTIDGRQVTVPEGTSVLEAAAQAGVNIPSLCYLKDINEI
                     GSCRVCVVEIEGARALAASCVTPAADGMVVHTNTKRVREARRAVLELIISNHPFECLT
                     CVRSGSCELQSLAEATGIREIRFEGAKSEHPVDDTTPSIVRDPSKCILCRRCVSVCEK
                     VQGVSALGLAGRGFDSVVSPAGELPLNEAACALCGQCVLVCPTGALSEVDSTGAVWEA
                     LGDPKKHVVVQTAPAIRVALGEEFGAAPGSIVTGQMVAALRALGFDKVFDTNFTADLT
                     ILEEGNELLHRLSEGGKLPMITSCSPGWIKFIEHFYPDMLDHLSTCKSPQQMFGALAK
                     TYYAEKAGIDPKDMFVVSIMPCTAKKFECTRPEMRSSGYQDVDAVLTTRELARMLREI
                     GVDPMELEPEEYDAPLGIGTGAAVIFGATGGVMEAALRTVYEVVTEKELESVDFTDVR
                     GFEGIKEAEVDLDGTVVRVAVAHGLGNARKLLDRVKSGEASYEFIEIMACPGGCIGGG
                     GQPIGTNTERRLERIDGIYEADRELPLRKSHENPAVKALYEEFLGEPLGEKSHELLHT
                     HYTPRGRWHWAE"
     gene            9719..>10144
                     /locus_tag="PLU15_09295"
     CDS             9719..>10144
                     /locus_tag="PLU15_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31152.1"
                     /translation="MFGKVGTFTNLGFAHTEEETRLDGYLQLMREQETCGKTNEAKKL
                     GISLSCVLSPNKVYKAYYRTDMGQISKRFIGASASYSLPNSQKILADLSLERAATGKA
                     PIYRIDIGYSACFDIPFSIKRDKEVVKGVVRRSEDHGYLG"
BASE COUNT         2317 a         2420 c         3135 g         2272 t
ORIGIN      
        1 caactgtgag cgttggggtc gcttcttacc cggaggcggg atccactgtg gacgaacttg
       61 tcagagctgt ggatgccgcc atgtacaagg cgaaacggca ggggaagaac agggtggttt
      121 gtgtctgacc ctgtcgtgat cttatcgtta tgattggcta ctaggtcagc ctttttatgt
      181 tgtctctggg attgcccggc agcatctggg caacaggaag ttcgaggaga gtatatgctc
      241 ctggttcgct tttgagtgcg gctctgaggc ctcccaacat ggcttgtgca gtgacggctg
      301 ggtttgatat cttcatctgc cattcaagga gctgattgga tgcttcccct gcggtcccct
      361 ttctgctgat ttctactgcg tgtgcgctgt ctgctagggg cgccacagac gcaacggaga
      421 agacctctgt gtcatcatct gagaaatatg gatctcgggt gatgtctctg acaatttcct
      481 ggaaaggcct ggaaccgtct tcttcgacgt agaccgttcg cctgtgtttg ccgtatcctg
      541 caggcaatgt gattgagatc gcgtcacgca ccccggccac agaacgtgcg acgactgagt
      601 ggcccatgct catgcccggg ccgaagttcg tatatgttat tccccgaggc gccatcaatt
      661 ccataagggc ccttataaga gaatcgatgc cggggtccca gcctgaggct atcaccgcaa
      721 ccgccctgcc tgcaatcgcc gaggagtgca gggcttcttt gtgctggcag atggcatctc
      781 cgtgtatgtc aaaggcatcg accacgctta tccctgaccg gagaagccca tgctccattc
      841 tctccagctc ccgtgtgggc acgcacatca gcgcagcttg aactcctcca agatgcgaaa
      901 tgtcggtgac ggctggggca gcccagttcc tgccgcgaat cagctcatcg gagacgacct
      961 ttcgccttac tattccagtc aactcgaagt ccggtgaagc cgcaatagcg tcggctgccg
     1021 ctcttcccac tttcccataa cctacgatgg cgaccggtgt gcgttccatt taggcaggaa
     1081 gcctccttta aaattgcttg gcttaacagg attatatagc tgacagcgtg cgccgtccag
     1141 attacatgat ataatgaacc tgtgcatgat gcagttgcgc attaacagac ggattcgggg
     1201 gactgtaatt gcaggtcatc gtagaacggg ctgagagatg tagaagatgt catgtttgtg
     1261 ttacggcgtg ccctgttggg gctatagtca tcagcagtgg cgaggttcgg ttgaggcgcg
     1321 acctgtgcat cgcctgtggg caatgtgtga aggcctgtcc caacggcgca ttggccatta
     1381 gggatcaggg cgaggatgta atggccctca tagggaatgg acgcactgtc gcgatattgg
     1441 atcctagttt cccgggggct tttcctcaat ccgatccgct ctgcctaatt ggcgccctca
     1501 gagagctggg tttcagctct atatgggagg catcgttcgg cgctgagtcc ctgcttccgt
     1561 actacgccga ctatgtgaag gcacattcgg aggaatcggg ccggtcggtt gcgtttgcat
     1621 ctatgtgccc tgctgtagtt gcctttattg aacattgccg tcctgagctt gctggtaatc
     1681 tagtccctct tgtttctcca actgtagccg cgtctcgtgt ggctagggct ttgggggaaa
     1741 agagggtcgt ttttattggt ccatgtcctg cagaaaaact tgaagccggt tttgggggcg
     1801 tgcccggagg gccgaatatt gcgattactt atgcagaatt atcgcgtttg cttgataatg
     1861 taggagtatc tgtggatcaa tgccctgcct cgcttcctga tgtaagggcg agcggcgctg
     1921 gtcgcatgct tggattgccg gcggggttgc ttcgggccct cagaattagg gatgatgttg
     1981 cgccgcccgg catcttgctt gcagagggca aagaagagtg cctgaaactg gtggaagctt
     2041 cgctgtcggg tgcattaggc ggttcatttg tcgtagcatc gatctgccgt agctgcgtgg
     2101 atgggactgg ttttccagaa ggtgcttctt tgtgggatag aaggagagcc gtggttgact
     2161 acattaagga agcaagttct gtgggcagca tggaagtgga tggcgctttg cgcgctgtgt
     2221 ctgcgtcagc cgatttcaag aggcgttact tgcctagaaa gattcctctc gcgccaaaag
     2281 agactgatgt gcgcacggcg ttgctcggca tgggaatgag gagcccggag gacgaactag
     2341 actgcgggct atgcggattc cagtcctgcc gcgaaagggc aattgcggtg gcgcgaggga
     2401 cgactgaggc cagtgagtgt tttccgcgca ggaatgcaag gttatctgag gaatgtgccc
     2461 gtctgagtca gcttgcgtcc gacctcgatg caatcgtcaa cacttcctgc gagatctcct
     2521 atgtctgcga tccggatggc tggatagtaa gggcaagcag agcggctaac gacattatcg
     2581 gtgtagagaa tgcgaatctg cacaaggtta acatgaagaa tcttcctcgc agactatgcc
     2641 ctgatgttgc gatccaggct ttgcacgagg tcaagcgagt gatggccatt agggatttgc
     2701 caggcgggca taggttgctt gcggcggcca cccccatact tgatggcgac ggggcctgta
     2761 gaggcgttgc ggcgacgata acggatctga gcggggcagc cttggagatc aatacaactc
     2821 ccacggccaa cagcttcgcg actgacgctg attccgagga catggtagcc tacagtcctg
     2881 ctatggaaga gctcctcacc ttagttcata aagtagccaa cgtatcgtca actgtgctta
     2941 ttaccggaga gtctggcgtg ggcaaagagg ttgtggcgag gtatattcat aggacgggaa
     3001 acagggcatc ggggcctttt gtcaagataa actgcgctgc aataccacat acgcttcttg
     3061 agtcagagtt gttcgggtat gaggaagggg cctttactgg agcacggaag agcggcaagc
     3121 ctggtctaat ccaactggct tctggaggca ctctttttct agatgagatt tcagagatgc
     3181 ccctgggaat ccaagccaag cttcttcagg ttctgcagga aaaatgtctt gtccgagtgg
     3241 gcggtactga gcaagtcagg gtggatgcgc ggatcatggc agccacaaat agggatctga
     3301 gggctgcggt gaaagaggga cggttccgtg gagatctcta cttcagactc aatgtcatac
     3361 ctatccgggt tccgccactt agggagagat ctggcgatat cgcgcctcta acctatcact
     3421 ttattgaaaa gtgggatagc agatacgggc ggaatcatca gatttcacgg gaggcctgct
     3481 cgcttttgga atgctataga tggcctggca acgttaggga actcgagaat cttatagagt
     3541 ttcttgtagt tactgttgat gaccccgtta tcctgcctga acatcttcca cagcacgtcg
     3601 tggcggggga gggcgagaga gatgtccagg tgagcgtgtc agggatcatg cctctccgtt
     3661 gggctgtgga ggaagttgaa cgccagctga ttgctcttgc cgctgaagca tgctctacca
     3721 cctattccat ggccaaagct ttgggggtta accagtcgac tgtagtgaga aaaatgcaga
     3781 agtacatgga gactcccgag gaggaggcag aggagaccgg gaacgtgtaa tgcgtttggg
     3841 cattaatcga tgcaacacag cattaagctt tgcaaaggca agcggagtaa ggtaatatag
     3901 gccgtgagca gggatgtcgt gcggacaaac gaaggagaca tgtgggcaca aatgtgaaaa
     3961 ggatcacgac tgatgcacaa gcgcattagc gagattatac tctgaaaaca aaagcgcagg
     4021 taggttgctt aagatccgcg tggcatggaa tatgcattga tataatatcg gccatcatgg
     4081 ccggcagagg aggttgaaag gtcgatgggt tgctgtgaat ccaagtgcgt cggcgatatc
     4141 acgcctgagc agtgggctca ggtagatggg ataatcgcca agtacaaggg ccagcgcgga
     4201 atcttgatcc cgtgtctgca cgagattcaa gaagcagtgg gctaccttcc cctggaggtt
     4261 caggagaggg tggctgcggg actcaatgtt ccgttggctg acgtctatag cgtcgttacc
     4321 ttctacgcgc tgttttccac caagcccaag ggcaaacatc aggtaggagt ctgcctggga
     4381 acagcgtgct acgtgcgtgg aggcgacaag attctcgaaa aggtagaaca ggcgctgggg
     4441 atcaaggctg gagacacaac tgatgacagg atgttctcga ttcaggtgat gaggtgtatt
     4501 ggcgcctgcg gtcttggccc agtcatgacg gttgatgatg acatctatgc gcgcatcaag
     4561 cccgaccgcg tggctgaggt tttggcgaag tattcctgat aaggatggca gagcaaggga
     4621 acgagggagg aatacgaatg gatttggtta gagcgcacgt ccttgtttgc ggcggcgcag
     4681 cctgtgtgtc ctccgggtgt gcagaggttc gcgaggctct ggaggctgaa atagccgcga
     4741 aaggcattgg gcgcgagatt aaagttgtcg tcacaggatg catgggacca tgcgatcttg
     4801 gtccgataat cgttgtttat cctgaaggcg ttctttactg caattgtacg cctgatgatg
     4861 ccagggagat agttgatgag catcttgtga aaggcaggat tgtaagtcga ctcctttatg
     4921 aggaaccgat gacatcggaa aaggtggaaa ctcaggaagg catgactttc ttcagccggc
     4981 aggaacgaat agctcttcga aacaccggag taatcgatcc gctggagata gatgagtata
     5041 ttgccagaga cggttatgcc gctttgggca aggttcttac agagatgacg cctgacgatg
     5101 tagtcgatgt gataaagcgt tcgggcctca ggggccgggg tggcgctggg ttccccacag
     5161 ggactaagtg gctgctgaca gccaaggcgc cgggttctcc aaaatacgtg gtgtgtaatg
     5221 cggacgaggg agacccaggt gctttcatgg acaggtcagt gctggaaggc gatccccatg
     5281 cagtgctgga ggccatgacg attgcaggct atgcggttgg tgcaaaccag ggctatatat
     5341 acgtaagggc agagtatcct ttggccgttg agcacctgca acatgcgata ggacaagcgc
     5401 gcgaatacgg cttgctgggc aaggatattc tcggatccgg attcgatttc gatatcgacc
     5461 tccgggttgg ggcaggtgct tttgtgtgcg gtgaggaaac cgctcttctg gcgtctatag
     5521 agagtcggcg aggcgagccg aggccgaggc ctccatatcc tgctcagtca ggtctgtggg
     5581 ggcagccgac gttgataaac aacgttgaaa catatgccaa cattgcgccg attattctca
     5641 aaggcccaga atggttttca tctatcggga ccgaaaagag caagggcaca aaggtatttg
     5701 ctcttgcagg caagataaac aacacgggac ttgtggaagt cccgatgggg acgccactgg
     5761 gcgacattat cttcgacata ggcggaggca tccccaatgg caagaagttc aaggccgcac
     5821 agaccggagg tccctccgga ggatgcatcc ctgctcagta tctaaacaca cctatcgatt
     5881 acgaatccct gaaagaactg ggcacgatga tgggctcagg agggctcatt gtcatggatg
     5941 aggacacctg catggttgac ctcgctaagt tctttatgca attcatacag gatgagtcat
     6001 gcgggaaatg tgcgccttgt cggataggca ccaagcggat gcttgagatc ctcgatcgca
     6061 taactaaggg acagggtagg gaaggcgacg tcgagctcct gatggaactg ggagagcaga
     6121 tcatggccac tgccatgtgt ggcctgggac agagcgctcc caacccggtt ttgtccacaa
     6181 ttcgctactt ccgcgatgag tatgacgctc acatcaaaga ccacaagtgc ccggcgtcat
     6241 cgtgcgcggc actcttctac tctccgtgcc agaactcatg ccctgctgat gtagatgtgc
     6301 ctcgctacat tgctctgata aaggaacgaa agttcgctga agcggctgcg ctgatccgtt
     6361 ccaagaaccc gttccccgct atctgcgggc gggtctgcga ccacccgtgc gaatccaagt
     6421 gcagacgtgc tacattcgac gagcctgtag cgatcaagtc tctcaagaga tttgctgcgg
     6481 attgggagaa gaaacatggc gtgtcacctg tggcggagca caagccgccg acagggaaac
     6541 gcgttgcaat agtgggtgcc ggtcccgcgg gccttagcgc cgcctactat ctcgcactag
     6601 ccgggcacaa ggttactgtg ttcgaggctt tgcctgtcgc cggcggcatg ctggctgtag
     6661 ggatccccga gtacaggctc cccaaggata tcttggaatg ggaaatcgag gtaatcaaga
     6721 aggctggcgt ggagatacga acaaacacgc ggatcggaga agatataaaa cttgacgagt
     6781 tgtcatcgga atacgatgcc ttattcatag cggttggcgc tcataatgaa gtgaagatgg
     6841 atgttcccgg ggaggacttc gaaggagtca tgcctgcagt ggctttcctc cgagatgtgg
     6901 cgctcggcaa gcctgttgag gtgggaaaga aggtggcggt cgttggcggc ggaaacgctg
     6961 cgatcgatgc ggctcgcacg gcgcggcgaa tgggcgccga agaggttcac atcctttacc
     7021 gcagactccg ggcggatatg cctgctgatg caggcgagat tgaggctgcc gaggaagagg
     7081 gcatccacat ccactaccta accgccccca ccaggatcat tggccgggac gggaaggtgg
     7141 ctcagatcga atgcgtcagg atgaagcttg gcgagttcga caagtcgggt agaaggcgcc
     7201 caattcctgt ggatgcatcg cgcacgatag tcgacatcga taccgtcata cccgctattg
     7261 ggcaggcagt cgacacatcg ttcataatgc cgctgtctga tacgggagaa aatctcttta
     7321 cgacgaaatc aggcttgctc atcacggacc ctgattcgtt ggagaccaat gttcccggga
     7381 tctttgcggg cggggattgc gtgacgggac catggagtgt cgtggctgcg attggcgcag
     7441 gacgcgaggc agcatacagc atagacaggt ttctcggggg cgatgcctgg attggagctc
     7501 atgccgaaat cgagcgctgc ttagtcggcg aaccgatcga ggaggagtgc aagcgttgtc
     7561 cgatgccgga attgcccgtc tctgagcgcg caaggaactt caaggaagtc gaacttggat
     7621 tcacagagga tatggcggtg gcggaagctg ctcgctgctt ccagtgtgac gtccgcgagc
     7681 cgtaagacca cgagaatgga gtgtgatagg aatgggtgat gttactctca ccattgacgg
     7741 acggcaagtc actgtcccgg aaggcacgag cgttctcgag gccgcagcac aggcgggcgt
     7801 caatatcccc agcctctgct acctcaagga cataaatgag ataggttcgt gcagagtgtg
     7861 tgtagtcgaa atagaagggg ccagagctct ggcagcgtcg tgtgtaaccc ctgccgccga
     7921 cggtatggta gtacatacca acaccaaacg cgtgcgggag gcgcgtcgcg cagtgctgga
     7981 gcttatcata tctaaccatc ctttcgagtg cctcacgtgc gtcagaagcg ggtcctgcga
     8041 gctgcagagc cttgccgagg caacgggcat tagggagata aggtttgaag gcgcaaaatc
     8101 ggagcatccc gtggatgata ccaccccatc gatagttcgg gatccaagta agtgcatcct
     8161 gtgccgaagg tgcgtgtcgg tctgcgagaa agtgcagggg gtgtccgccc tcggacttgc
     8221 agggcgagga ttcgactccg tggtgagccc cgccggcgag ctgccgttga atgaggcggc
     8281 atgtgccctc tgtgggcagt gtgttcttgt gtgtccaact ggagcgctgt cagaagtgga
     8341 ctccacaggc gcggtctggg aagcgctggg agatccgaag aagcatgtgg tggtgcagac
     8401 tgcgccagct attagagtag cgttgggtga ggagttcgga gcggctccgg gctcgatagt
     8461 tactgggcaa atggttgcgg cactgcgcgc cctcggcttc gacaaggtgt ttgacaccaa
     8521 cttcacggcc gacctcacga tacttgagga aggcaacgag ctgttgcaca ggctttcgga
     8581 aggcggcaag ctgccgatga tcacatcgtg cagtccggga tggataaagt tcatagagca
     8641 tttctacccc gacatgcttg accacctgtc tacatgcaag tcgccacagc agatgttcgg
     8701 agcgctagcg aaaacctact atgctgaaaa ggctggcata gacccgaaag acatgttcgt
     8761 ggtgtcgata atgccctgca cggcaaagaa gtttgagtgc acaaggcctg aaatgaggtc
     8821 gtcgggatac caggatgtcg acgcagtact taccacaagg gaactggcgc gcatgctgcg
     8881 cgagataggc gtagacccga tggagttgga acctgaagaa tacgacgctc cgctgggaat
     8941 cggaacagga gctgccgtga tcttcggagc caccggaggg gtcatggagg ctgctctgag
     9001 aaccgtatac gaagtggtga cagagaagga actcgagtct gtcgatttca ccgatgtcag
     9061 aggctttgag ggcataaagg aagccgaagt cgatctcgat ggcacagtgg tgcgcgtcgc
     9121 ggtcgctcac ggccttggca atgctcgaaa gcttctggac agggttaaat cgggcgaggc
     9181 gtcgtacgag ttcatcgaga tcatggcatg cccaggaggg tgcatcggcg gcggcggaca
     9241 gcctattgga acaaataccg agaggcgtct cgagaggatc gatggaatat atgaggcaga
     9301 ccgtgagttg ccgctcagga agtctcatga gaatccggca gtgaaggcct tgtacgagga
     9361 gttccttgga gagccgttgg gagagaagtc ccacgagctg ctgcataccc actatacgcc
     9421 gcgtggcaga tggcactggg ctgagtagac aggacacggt aggtgcaatg agggtcttga
     9481 cccgcgttgc gcttgagaaa tccgatgaaa aacaggcgaa aaacgtacgc atgggggcac
     9541 gccagttgac gtgccccctc ttagttttgc ttgacaggtg aacaaagact tccccacagc
     9601 tcgccttatg cagattcaga cccataatag tgggttgtct ggggtcgggg agtgggcttt
     9661 catctgagtg tttccaagct tcttttccag gtgtacatcg aaatgtgaag ccgaagttgt
     9721 gtttggaaaa gtcggcacat ttacgaatct gggctttgcc cacactgaag aagaaacaag
     9781 gctggatgga tatttacaat taatgaggga acaagaaacc tgcgggaaga ccaatgaagc
     9841 caagaaacta gggatttcgc tttcttgcgt actttctcca aacaaggtat acaaggcata
     9901 ctaccgcaca gacatggggc aaatctcaaa gcgcttcatt ggcgcaagtg caagctactc
     9961 actcccaaac tcccaaaaaa tacttgcaga tttgtccctt gaacgtgctg caacaggtaa
    10021 ggcgcctatc tatcgcatcg acattggcta ttcggcttgt tttgacattc cattctcaat
    10081 taagagagac aaagaggtag tcaaaggagt cgtccggaga tctgaggatc atggttatct
    10141 tggg
//
LOCUS       DAOSYL010000069        10023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5727_length_10023_cov_7.795546, whole genome shotgun sequence.
ACCESSION   DAOSYL010000069 DAOSYL010000000
VERSION     DAOSYL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5727_length_10023_cov_7.795546"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(658..1839)
                     /locus_tag="PLU15_09300"
     CDS             complement(658..1839)
                     /locus_tag="PLU15_09300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOO31153.1"
                     /translation="MLTYRAFKVLTSFVIGVVIGVCLRTACPVEVPALSPPYLSPPGR
                     PELIYHRAFREGPAVSARAAILVEASTGIVLYAKNEHERREQASITKAMTAIVAIERG
                     DLADIVRVSPNAAKVPGSTLNLVPGQDYKLEELLRATMLRSGNDGATAVAEHVGGSVE
                     DFVTLMNARAASMGLKNTHFANPHGLSAPGHYSTAYDIALMCMWGLRLPKFADIVGCP
                     ELWTSSVESGRAKVVYNTNRLLWGFSGADGIKTGTTSKAGHCLAASATRDGLQFIAVV
                     LRSNARFKDAADLLEYGFSTFTRIRAAEAGVAVGDVLVRRGLDRSVEVATAKDVDVVV
                     RVDEVPGISVEIRVPESPEAPVCPGDRVGWLDVLYQGDLIASQQLYAMKAVDRRPIWA
                     R"
     gene            complement(1905..2369)
                     /locus_tag="PLU15_09305"
     CDS             complement(1905..2369)
                     /locus_tag="PLU15_09305"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOO31154.1"
                     /translation="MVAAQPERDELLNGKRLACIVLECVDSIPQPVGRTKIALLLKGS
                     RSQQIRTIDIRQVRHFGALGHLTIAQIQGIIEQLLSEGYLGSSYGKRPVIHVTSLGRT
                     TMITGDMPEVTIPDELKLYVARERCRKCPHNPYITDGPGAGPNTPNDIPPPF"
     gene            complement(2439..4862)
                     /locus_tag="PLU15_09310"
     CDS             complement(2439..4862)
                     /locus_tag="PLU15_09310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOO31155.1"
                     /translation="MRRRGSRCHIGRLRWLVSAIALLAAMMLIPLGVVAGSPARERAS
                     AVLAGMHVQKLSLEIGPRPTGTMRETAAADYVADTLEGYGYTVTRVPFSRETADGVSV
                     SENVIAVAPSAKPDSKLILIGASLDTYGMDSTGADHNGSGLAAMLESARLLSGRLIGR
                     SQVAFVAFGSNEMGRAGSKAFTEGTAAGPIDPRNVAIMINIDAVGRGDSVRLIPWGGP
                     RALAPKAFERLVDAVADSTGKPVGFDTTIAMMPQVHTDHSPFLVAGVPAVTITTARSL
                     PYYSGNSLDPYLDVAAEVSGARVAMAADAVVAAADWAASAYRIGKPTGTYISLRVFGD
                     IISVSYITSVIVAAAAVVIGIACIAYSNSMLYDELSRVRSAGTVKAVGMALAVYAVMT
                     VVLWTAFLPSVVVEAVRGVERPWTAYPAPYILAGAVWALFFAILILSLTGTGVREPLR
                     APMLRLAMIIQTALVCLSVPILRAGSFPFSLGLIFTSLALLFPEGTARKIFACLAPVP
                     VVWLGCKAVVGVGNRIIADLLLVPIFLCLLVAALALPYAIAILVLATEPRGRRGTFYS
                     PGNLFYDIAPGTTLREYRVAPGSVPESRKRRPSARKTFMWILGVLALILTASLFLYPS
                     YNSRRPQPVLVTHVVDGLGGHSLVFESNDNIKGISVVSRSGERGPEKIDARSTSHLIP
                     YSFSAPRLSLVAEMDGPALWVRLLSSAPIRSVKICIVGPQGMAVLSSNRRCLIEEDGD
                     MVRISTVLSGEEPIEAQFELAFPPDAPLSVEATGSFWENPAPIIISGDGKRFYETNQF
                     VVATPITAK"
     gene            complement(4859..5116)
                     /locus_tag="PLU15_09315"
     CDS             complement(4859..5116)
                     /locus_tag="PLU15_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31156.1"
                     /translation="MGSGTARADFSGNPDAADIAVDPVAPAKPGRNIESRIGLGRDGR
                     EHDKECSTGMKMGFRELRDCAPCDRVVLTIGTGRIEVEHDR"
     gene            complement(5340..5636)
                     /locus_tag="PLU15_09320"
     CDS             complement(5340..5636)
                     /locus_tag="PLU15_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31157.1"
                     /translation="MGVAIGTALLPLAGVFLVLATAPDARRVADTDGNLTHKAFAVMR
                     RDFNHEELNLRMDMGSGRMKIMAGRGRNMLAAKAGYWSPANRPSCREKMNTKSP"
     gene            5820..6950
                     /locus_tag="PLU15_09325"
     CDS             5820..6950
                     /locus_tag="PLU15_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRK06851 family protein"
                     /protein_id="HOO31158.1"
                     /translation="MATVAQVRRMFAGGNTSGGFRSFYDNIIGQDATRTIVLKGGPGV
                     GKSTLMKYIAEHMADLGYDVELFHCSSDPSSLDGVVMPGIGVALMDGTAPHMVDPRFP
                     GAVDEILYLGDYWNESAMRAKKDEIINCSKDASRHFQRAYRFLRTAGDVLDDWSDANA
                     EALDAGAANKLCHQIIVDVFGQTSVADHPGKKRSLFASAITPDGFVNYLDTVVAGCLR
                     RFVIHGGPGSGKSTLIGHVADHAIRSGLDVELFHCSLNPESLEHLVVPAIKTALITSV
                     EPHVWTPRKDDIVIDMEQCLNRTVVERNARIVDYDRSLMQEAMDRAIFFLGQARDAHR
                     FLESLYTPNIDFDAVKGLRERTLNRILEYASESRQVAPEHGN"
     gene            complement(7039..9597)
                     /locus_tag="PLU15_09330"
     CDS             complement(7039..9597)
                     /locus_tag="PLU15_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO31159.1"
                     /translation="MQDRKHSKARNKESSRKRRGGAGAPTARQKQQPGPAKSRSRKSS
                     DLHGPGNAKRPTQHGSDHRRKKELSRSVAAFAPDIEFPVSEEERAAVPVEYRGLKLDP
                     FQQEAIRHLEAGRSVLVSAPTGVGKTLVADYLIERMYNEKSRVIYTAPIKALSNQKFK
                     EFKRLLGEENVGIITGDVVINSSAQILIMTTEIFRNMLHLSPHALDGVSHVIFDEIHF
                     ISDEDRGTVWEESIIFMPEQMRLLGLSATIPNAGELACWMREIKGHDVAVVRHFKRVV
                     PLRHFVYEKSRGACTLKEMRQYKAERDREIEEAEALAAGAGPRYQTTTHVDLVNYLTE
                     EQKLPCLYFVFSRRQCEEKAEELARTANYLSFAERKEVLSFFDERIEGMNIESMPSVK
                     KMRRILGRGIAYHHAGLLPVLKEIVEDLFERRMVQVLYATETFAVGINFPVKTVCFDG
                     VRKFNGVDFAPMSAQEYFQMAGRAGRRGIDPEGYVYILVDLNYFRPEEYPSTDERSVE
                     PLVSRFTISYNTVVNLIATRSPEEIRKVLKQNFASYQRDAERLEIEEALSSARAMESE
                     IIAEACELLDKPGCPLYAGKLRSRLAETRVQLKRASKRKRSRGKRAALRVEMDEIRAE
                     LGSLDTRECEVARRKACRKLLSKRGVVVGRMENLYERMRRLGSEERYVHEFRRKQALL
                     ERLSYVQDGVLLPRGEICREIHTQELLVTELIFAGVFHEFDIDQIAALAVCIDYEPRR
                     GEFAAKSVPFDLARIREVAEAIEVVEESFVGTSSLQFFTGLAPLAYRWSQGEDFADLM
                     KGSSYAEGDVVSSFRRAIDLLRQVRAACKDDLALADKITETIRRIDRDVVEVVL"
BASE COUNT         2256 a         2881 c         2595 g         2291 t
ORIGIN      
        1 agtgtatggg gagaagtaga ggttggcaac gaacctggtc aactttgagt cacatcccat
       61 tgaggcgaac cagcggggat tctcgtggat atgttcaatg cgtaacgctc gcaaaagcca
      121 gtccaacgcg tgccaacagg ctttttttga gttttcccca aaagcccata cgtgtgcagg
      181 caaaacccaa gatcaagctg tgccgattac ttgacctgcc ttcgctaaaa acaacctcga
      241 actgagcttt atacacattc ccatcgggca actttccctg tgcggtatcc acgcgatagg
      301 cgtttgaaag ggttcagcta ctctttcaaa gctacttgtt gtcgcagacc gttgcgggac
      361 caattcgcgc tcttatgcac attcagctga ctgacatcac cgtcaaattg ggcagcacaa
      421 agctgtaaac cgccaacaag ctatctgcgc cacacggaac gcgtagtctt ggatagcaga
      481 atagcgcttt acagtatgtt cctttacttc aaccccaagc catccagcgc gattcttgac
      541 cggccgcaca gtcagtaaca gtcagccgaa gcatgcagca gaaccaattg cccttgccat
      601 cagtcctcat gcagatcttt gaccggaagc cgcttgcggc ccgcagtttg gcagacatca
      661 acgtgcccat ataggcctgc ggtcgacagc cttcatagca tacagttgct ggctggcaat
      721 gaggtcacct tggtatagca cgtcgagcca ccctacgcga tcccctgggc atactggcgc
      781 ttccggcgat tcgggcaccc ttatttccac ggatattccc ggcacctcgt caacgcggac
      841 cactacatcg acatccttcg cagttgcaac ctccacagaa cgatcgagtc cacggcggac
      901 gagaacatcg cccacagcca ctccggcttc agccgccctt atccgcgtga acgtggagaa
      961 accgtattcc aacaagtctg ccgcatcttt gaatctggca ttggagcgaa ggacaacggc
     1021 tatgaactgc aacccatccc gggtcgcaga ggctgccaag caatgtcctg ctttggaagt
     1081 ggttcccgtc ttgatcccgt cggcgccaga aaaaccccaa agaagcctgt tggtgttgta
     1141 tactactttc gccctcccgc tttccacaga agatgtccaa agttcagggc atcccacaat
     1201 gtcggcgaat ttgggaagcc tcaaccccca catgcacatg agggctatgt cgtacgctgt
     1261 ggaatagtgt ccaggagcgg atagtccgtg cgggttggcg aaatgggtgt tcttcaagcc
     1321 catgcttgct gcgcgggcat tcatcaacgt cacgaaatct tctaccgatc cacctacatg
     1381 ctccgccaca gccgttgcgc cgtcatttcc tgacctcagc atggtagcgc ggagtaattc
     1441 ctcaagcttg taatcttgcc cagggacaag gttgagtgtc gatccgggaa ccttggctgc
     1501 attcggtgat acgcgcacga tatcagccag atcgccccgt tcaatggcga ctatggctgt
     1561 catggccttg gttatgctcg cctgttctct tcgttcatgt tcgtttttgg catagagcac
     1621 gatcccggtg gaggcttcga ctagtatggc ggctcgggcg ctcaccgcag gtccttcgcg
     1681 gaaagcgcgg tggtagatga gttcgggacg cccaggtggg ctgaggtatg ggggcgacag
     1741 tgcgggcacc tcaacgggac aggctgtgcg cagacagact ccgatgacca ccccgataac
     1801 aaatgatgtt aaaaccttga aggctctata agtgagcaat ttggcccagc gcccccgatc
     1861 agcccaagtt gactgcctta attcttacgc caagcgaagt ctagctagaa cggaggaggt
     1921 atgtcattgg gtgtgttagg gcctgcaccc gggccgtcag tgatataggg attatgaggg
     1981 catttgcgac atcgttcccg tgctacatag agtttgagct cgtcagggat ggtgacctcg
     2041 ggcatatcgc ctgtgatcat tgttgtacgt cccaacgacg taacatggat caccggtcgt
     2101 ttcccatagg atgatccgag gtatccctct gaaagaagct gctcgattat cccttggatc
     2161 tgggcgattg taagatggcc caaggcacca aaatgcctga cctgtctaat gtcgattgtg
     2221 cgaatctgtt gtgatctaga cccctttaac aagagagcta tcttggtgcg tccgacaggc
     2281 tgaggtattg agtccacaca ttccagcacg atgcaggcaa gacgcttgcc attgagcaac
     2341 tcgtcacgct ccggctgtgc cgcaaccaca tcgtcacctc cagtataagt tattgcacca
     2401 acagattacc ataggtccct ttagatgtca actgtatcct attttgcggt tataggcgta
     2461 gcaactacaa actgatttgt ttcataaaac ctcttaccat ctccagatat tataattgga
     2521 gcagggttct cccagaatga tcctgtcgct tccactgaga gcggtgcgtc gggcggaaac
     2581 gccaattcga actgggcttc gatcggctct tctcccgata acacggtgct gattcgcacc
     2641 atatcgccat cttcttcaat aagacagcgg cggttgctag agagtaccgc catcccttga
     2701 ggacctacaa tgcatatctt aacggaacga ataggggcac tcgataacag cctaacccag
     2761 agcgcgggac cgtccatctc tgcaacgagg ctcagccgcg gagcactgaa tgaatatggt
     2821 atgagatgcg aggtggatct cgcgtctatc ttttcggggc ctctctctcc agagcgcgaa
     2881 acgacagata tccccttgat gttgtcattg ctttcaaata ctaggctatg ccctccaagt
     2941 ccgtccacta catgcgtaac caatactggc tggggccttc tcgaattata gctggggtac
     3001 aaaaacaggc tggcggtcaa tatcaaagcc aacacaccca gaatccacat gaaagtcttg
     3061 cgagcggagg gcctcctctt cctgctttct ggaactgaac ctggagcaac tcggtactct
     3121 cgtagcgtcg tgccgggggc aatgtcgtag aacaggttgc caggggaata gaaggtccct
     3181 ctccttcccc tcggttccgt tgccaggacg agtatggcga ttgcatatgg cagcgcgagc
     3241 gccgcaacga gaaggcagag gaatatggga accaacaaga ggtccgcaat aatccggttt
     3301 cccacgccaa caacggcctt gcacccaagc cacactacgg gcaccggagc aaggcaagca
     3361 aagatcttcc ttgccgttcc ttccggaaac aagagagcaa gcgacgtaaa gatgagcccg
     3421 agagagaacg ggaaagaccc ggcccgtagg ataggcacgg aaaggcagac cagggctgtc
     3481 tgtataatca tggcaaggcg aagcatcggg gcgcgaagag gctctcgtac accagtacct
     3541 gtaagggata gtatgagaat tgcgaagaag agtgcccaca ctgcgccagc gagaatgtag
     3601 ggagcgggat atgcggtcca cggccgctcg acgcctcgca ctgcttcaac cacgacagac
     3661 ggaagaaagg cggtccaaag aaccacagtc attaccgcat aaaccgccag cgccattccc
     3721 acggccttga ctgtcccggc agatcttact ctggaaagct catcatatag catgctgttg
     3781 ctatatgcga tgcaagcaat cccaatcacg actgctgcag ccgccacgat cacactggtt
     3841 atgtaggaga ctgagataat atccccgaat actcttaggg agatataggt cccagtgggc
     3901 ttcccaattc tgtaggcgga ggctgcccaa tctgcagcag ccactacagc atctgcagcc
     3961 atggccactc tcgcaccgct cacttcagca gcaacatcca agtatgggtc gagtgaattg
     4021 cctgagtaat acgggagcga acgcgcagtg gtaatggtta cagccggaac gcctgccacc
     4081 aggaaaggtg agtggtcagt gtgcacctgc ggcatcatgg ctatggtcgt gtcgaaccct
     4141 accggttttc cagtggaatc ggccacggca tccaccagcc gctcaaatgc cttgggggct
     4201 aacgcccggg gccctcccca tggtataagg cgcaccgaat ctccacggcc tactgcatct
     4261 atgttgatca tgatcgcaac gttgcgggga tcgatggggc cggcggctgt gccctcggta
     4321 aatgccttcg acccggcccg ccccatctcg ttggatccga atgccacaaa agcaacttgc
     4381 gagcggccaa tcagccggcc agataggagc cttgccgact ccagcatggc ggcgagtcca
     4441 gaaccgttat ggtcggcacc ggttgaatcc attccataag tgtcaagtga tgctccgatc
     4501 aggatcagct tcgaatcagg cttggccgaa ggtgcaacgg ctatgacgtt ttcagatact
     4561 gaaaccccat ccgcagtttc gcgtgaaaat gggactcttg tcacggtgta cccataccct
     4621 tccaatgtat ctgcaacata gtccgcggct gcggtctccc tcatcgtacc ggttggcctc
     4681 ggaccgatct cgagcgaaag cttctgcaca tgcattcccg ccaaaacagc actcgccctc
     4741 tcccgggcag ggctgcccgc gacaacgccc agcgggatca gcatcattgc tgctagcaaa
     4801 gcaatcgctg aaacaagcca cctgaggcgg cctatatgac aacgacttcc tctacgtctc
     4861 aacggtcatg ttccacctct attctgccgg ttccgatcgt gagaaccact ctatcacacg
     4921 gagcgcagtc tcgtagttcg cggaaaccca tcttcatccc cgtgctacac tctttgtcat
     4981 gctcgcgccc atctcttccc aggccgatcc gggattcaat gtttcgcccg ggtttcgcag
     5041 gggccacagg gtcgaccgcg atatcagctg catcgggatt tcctgaaaag tctgcgcgag
     5101 ctgtgccgga tcccactttt tcccgtgctc tatctattcc cgaattacct agtcaggaga
     5161 tgtcggcctc tttgggccaa aagcccgcga gttgtctcaa gccctgtgcg gtaaaggaga
     5221 acacgaactt tccacttacg actcgaactt caacgtcgcg cacgtcaagt ccttcaatga
     5281 ttccatcgac actgccgagg ccaaccttga tgtcgaagaa gtagtcagag caggctcacc
     5341 tacggcgatt tcgtgttcat cttttcacgg cacgacggtc tattcgctgg cgaccaataa
     5401 ccggccttgg ctgcaagcat gttccggccg cgtccagcca tgatcttcat ccttcccgaa
     5461 cccatgtcca ttctgaggtt gagttcctca tggttaaagt ctctccgcat aacagcaaac
     5521 gctttgtgcg tgaggttgcc gtctgtatcc gcaactcgcc gggcgtcagg ggccgtggca
     5581 agaaccaaaa atacgccggc gagtggcaga agggctgtgc caatggcaac gcccattttt
     5641 ctgtttctgg caatcctgta tattcagcgg aagaagaacc ccggccaatc gattttcacg
     5701 ccattctcct cctcgccggt acactccatc ggttctccaa gttttcttcg ttctgccgta
     5761 cacatggacc caatttggca taggctaagt gtgacctggt tattggaggg aatcagatta
     5821 tggccactgt agcccaggtg aggcgcatgt ttgccggagg caatacgtcc ggcggttttc
     5881 gctccttcta tgacaacata ataggacaag atgctaccag aaccatagtc ctaaaaggag
     5941 gccccggagt cggaaagtca actctgatga agtacattgc cgaacacatg gccgatctcg
     6001 gttacgatgt cgagctcttt cactgctcct cggatccgtc ttctctggac ggcgtggtta
     6061 tgccgggaat cggtgtcgcg ctcatggatg ggaccgcccc tcacatggta gatccccgtt
     6121 ttccaggggc tgtagacgaa attctgtatc tgggagacta ctggaacgaa tctgcgatgc
     6181 gtgccaagaa ggacgagata ataaactgct ccaaagatgc atcccgccac tttcagcgcg
     6241 cctacaggtt cctccggact gcaggggacg tgctagatga ctggagcgac gcaaatgctg
     6301 aagcgcttga tgcaggtgcc gccaacaagc tctgccacca gataatcgtg gacgttttcg
     6361 gacagacgag cgtggctgac caccccggca agaaacgcag cctctttgca tcggcgataa
     6421 cacccgacgg cttcgtcaac tacctcgaca cggtggtagc aggctgcttg aggcggtttg
     6481 tcatccacgg aggccctgga tccggaaaat ctaccctcat cgggcacgta gccgaccacg
     6541 cgatccgttc agggctagat gtggagcttt tccattgttc gctcaacccc gaaagtctcg
     6601 agcaccttgt ggtacctgct atcaaaacag cactgatcac gtctgtcgaa ccgcatgtgt
     6661 ggacccctcg caaagacgac atcgtcatag acatggagca atgtctcaat cgaacagttg
     6721 tagaacgcaa cgcaaggata gtggattacg accgcagcct aatgcaagag gcgatggacc
     6781 gagcaatatt cttcctcggc caggcgcggg acgcgcaccg ttttctggaa tccctataca
     6841 ctcccaacat cgattttgat gctgtcaaag ggctaaggga acgaactctc aatagaatac
     6901 tcgagtatgc aagtgaatct cgccaggttg ccccggagca cgggaactga acaggtccca
     6961 acagttcact gcggaatcct ggcatgtgcg ctacctgttg ttggggtatt agttgcctaa
     7021 tgaaagccga gataatttct atagcactac ctctacaaca tccctgtcga tccgtcttat
     7081 ggtctccgtt atcttgtcag ccagagcgag gtcgtctttg cacgctgccc gcacttgtcg
     7141 gagaaggtcg atggcccgcc ggaaactgga gactacgtcg ccttcggcat aacttgaacc
     7201 cttcatcaag tcagcgaagt cctcgccctg gctccatctg taagcaagcg gggcaaggcc
     7261 ggtgaaaaac tggagtgagc ttgtgcctac aaagctctct tcaacaacct ctatagcttc
     7321 ggctacttca cggattcggg caagatcgaa cgggactgac ttcgcagcga actctccgcg
     7381 ccgcggctca taatcgatgc aaaccgccaa tgcagcgatt tggtctatgt cgaactcgtg
     7441 aaagacgcct gcaaaaatca gctcagtcac aaggagttcc tgcgtgtgta tttcgcggca
     7501 tatttccccc ctaggcaaaa gaacgccgtc ttgcacgtaa ctgagtcttt caaggagtgc
     7561 ctgtttcctt ctgaactcgt gcacatacct ttcctcgcta cctagcctac gcatgcgctc
     7621 gtatagattc tccatgcggc cgactacgac tcccctctta gacagcagtt ttcggcaggc
     7681 cttcctccga gcgacctcgc attcgcgggt atccagtgaa ccgagctcag ctcggatctc
     7741 gtccatttca actctgagcg cagcgcgttt gccgcggctt cttttccttt tggaggctct
     7801 cttcagttga acccgggtct ccgcgagacg ggaacggagt tttccggcgt agagcggaca
     7861 tcccggtttg tccagaagct cgcacgcttc cgcgatgatc tcggattcca ttgctcgcgc
     7921 agagctcaag gcctcctcta tttccaacct ctcggcatcc ctctgatagc tggcgaagtt
     7981 ctgcttcaag actttgcgta tttcctccgg cgaccgggtc gcgatcaggt tgacgacggt
     8041 gttgtatgat atggtaaatc ggctcaccaa aggctcgacg cttcgttcat cagtgctggg
     8101 atattcttcg ggacggaaat agttgaggtc cacgagtatg tacacgtagc cttcagggtc
     8161 tatgcccctc ctgcccgcgc gccccgccat ctggaagtat tcctgggcgc tcatgggcgc
     8221 gaaatcgacg ccgttgaact tgcggactcc gtcaaagcac acagttttca cagggaagtt
     8281 gatgcccacg gcaaaggtct cagtagcgta gagaacctgc accatcctgc gctcgaagag
     8341 gtcttcaacg atctctttga gaaccggcaa aagcccggcg tgatggtatg cgatccccct
     8401 acccagtatt cggcgcatct tcttaacgct cggcatcgac tcgatattca ttccttcgat
     8461 tctctcatcg aaaaaggaaa ggacttcttt tcgttcggca aagctcaagt aatttgccgt
     8521 tcttgccagt tcttcggctt tttcctcaca ctgtctccgg ctgaagacga agtaaaggca
     8581 tggcaacttc tgttcctccg tgagataatt cacgaggtcg acatgggttg tcgtctggta
     8641 tctcgggccc gctccagccg ccaaagcttc ggcctcttcg atttcccggt ctcgttcggc
     8701 cttgtactgc ctcatctcct tcaatgtgca tgcgccgcgc gatttctcgt acacaaagtg
     8761 tctcagggga actacgcgct taaaatggcg gactactgcc acatcatgtc cctttatctc
     8821 ccgcatccag caggccagct cgcctgcgtt gggtatggtt gcagagagcc cgaggagcct
     8881 catctgctcc ggcatgaata taatcgactc ctcccacacc gtgccgcggt cctcatcgga
     8941 aatgaagtgg atttcgtcga atattacgtg ggagacgcca tctagggcgt ggggactcaa
     9001 atggagcatg ttccggaaga tctccgtggt catgatcagt atctgggccg atgagttgat
     9061 gacgacgtcg cccgttatga tcccgacgtt ctcctcgccc aaaagccgtt tgaactcctt
     9121 gaatttctgg ttcgacaagg ccttgatggg cgcagtatat atcactctgc tcttttcgtt
     9181 gtacatacgc tcgatgagat agtcggccac caatgttttg ccgacgcctg taggagcaga
     9241 aacgaggacg gatcttccgg cctcaagatg cctgatggct tcctgctgga agggatcgag
     9301 cttcaatccc ctgtattcta cgggcacagc cgcgcgctcc tcctcggaaa cagggaattc
     9361 gatatccggc gcaaaagccg ccacagaacg cgacaattcc tttttgcgtc gatgatcaga
     9421 gccatgctgt gtcggccttt tggcattgcc ggggccgtgc aagtccgaag atttccttga
     9481 acggctcttc gccgggcccg gctgttgctt ctgcctagcc gtcggcgcgc ccgctccgcc
     9541 gcgtctcttg cgcgacgact ccttgttgcg tgccttggaa tgttttcgat cctgcattaa
     9601 tttatcacct cagtagagac aacgcggacc cgagtccgct ctgctttgaa aggcaagccc
     9661 tggctgccat cttgagtata caggttctcg gtgcctggga caattcagtc ggcgatattc
     9721 gaaagcggtc gcaccgccca gcccggccta tatggtttag tacagcccca tgccagcccc
     9781 aactattcaa ggcatcgcaa tcgaggcgcg tttcgcggag gctcggagac aatcctattc
     9841 gcctgcagaa aaacccggag cctcttctga aaaattgcta taggaaaagc cgatgcggaa
     9901 ttggaggtag tgattatgga cccgagggtg tgggagctgg caaagattct tgtagactac
     9961 tcagtcagtg tccaagatgg cgatgtggtg ataatcagcg cggatctcga cggttcgcag
    10021 ctg
//
LOCUS       DAOSYL010000070         9971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5769_length_9971_cov_8.855486, whole genome shotgun sequence.
ACCESSION   DAOSYL010000070 DAOSYL010000000
VERSION     DAOSYL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5769_length_9971_cov_8.855486"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLU15_09335"
     CDS             <1..384
                     /locus_tag="PLU15_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO31160.1"
                     /translation="IGIGVCPGKRTTVSWTPGFGSLEVEIGFQPYPISPKDVAVSIED
                     TSATVSWTPVEESMVDHYRVYWRYSTQERYITSRSVVVEAPASSAAVSLSSRQRGSKV
                     RFVVVARDAQGQESLRSDEAVAEYR"
     gene            complement(455..1804)
                     /gene="mgtE"
                     /locus_tag="PLU15_09340"
     CDS             complement(455..1804)
                     /gene="mgtE"
                     /locus_tag="PLU15_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOO31161.1"
                     /translation="MDETYENIIEHMRALLATGTDEALIEFATTLHPADIVDLLEELD
                     DAERSAFFHVIPADVAADIVQELEPADQAELIRLMGAARSSEILKEMDSDDAADLIAE
                     LPEAKAETLLDLMNSHGQDVRELLQYDEHSSGGIMATEYVSIQSDWTVEQTLAELRRV
                     APEAETVYYIYVLDDDEHLVGVLSLRDLVVADLSTRIDAIMNTNVLSVPVEADQEDVA
                     QLFKKYRFLALPVVDNEQRLTGIITADDILDVFEEEATEDMQKIAGTMPLERPYFSTS
                     IKELWGSRIVWLLVLFIAESFTGGIMRQFSDAMQSVIELTFFIPLLIDCGGNAGSQAS
                     TVVIRGLAVRDIRLRDVWTVMARELVVGLGLGVVMAGVGFFRAWRVGGSSDIGIIVCL
                     AVLAIVVLSTVIGALLPIIATSLKVDPAVMSAPLITTIVDAFGLLIYFSIAKSVLQL"
     gene            2891..3844
                     /locus_tag="PLU15_09345"
     CDS             2891..3844
                     /locus_tag="PLU15_09345"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOO31162.1"
                     /translation="MSDLREAALIAVRDCMGARSGEDVLVIMDKGTRTVGMALFEAAV
                     SLGCEAMVVEIIPRKMNGEEPPEPVGEMMKHVDIVLAPTSKSITHTVARGNACKAGAR
                     VATLPGITEDCMTRALVADYDEVAKRAEKYARILTDGKIVRITNADGTDISMSLEGRV
                     GMADTGIYRDPGQSGNLPAGEAFIAPVEGTANGVFVADGAMAGPEFAGTKIRITVANG
                     YATSIEGGEAARTIEKMISPLGMPARNIAELGIGVNDRAKITGQVLEDEKVMGTIHIA
                     LGDNSNFGGNVRVASHLDGIVVAPTVEVDGVVIMDSGKFTH"
     gene            3909..4124
                     /locus_tag="PLU15_09350"
     CDS             3909..4124
                     /locus_tag="PLU15_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31163.1"
                     /translation="MRKVIHKIPRQIRVAGFSGDRKRRPPDFTDSDVVDAILTEVEWE
                     EEGLPDTRLDGWWSDAWSQPAGKPEDR"
     gene            4393..7533
                     /locus_tag="PLU15_09355"
     CDS             4393..7533
                     /locus_tag="PLU15_09355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOO31164.1"
                     /translation="MDGACRPDELASRISELGMDSCAITDHGCLYGLVDFYRACNQQG
                     VKPILGCEVYVANRTLHDRTPHIDDNPYHLVLLAENDVGYSNLLKLSSLGYTEGFYYK
                     PRVDKKALAEHSEGLIALSACLSGEVPSYVAQGRIDEAEAVARQYLEIFGRDSFFLEI
                     QSNGMRVQDLVNRQLVQLGRKLGVPVVATNDVHYLRREDATAHDVLLCIQTGSVVDDP
                     SRMRFPTDQFYLKSAEEMYREFAEIPEACAMTARIAERCNVELDFDTLHMPEFEAPPG
                     QTLDSYLRRLCEEGAIRRFGAISREVRERLDYELGIIERMGYSGYFLIVWDFIRYAKS
                     QGIYVGPGRGSAPGSLAAYCLGITDVDPLAYNLLFERFLNPERVTMPDIDVDFCYERR
                     GEVIDYVAGKYGSDKVAQIITFGTMAARAAIRDVGRALKFSYAEVDRIAKLVPAELNM
                     TIDRALEVSPELAAAVGESERNRTLIEIARAVEGLPRHPSVHAAGVVISKDPLTDHVP
                     LYKSADGVLTTQYPMEDLERLGIVKMDFLGLRTLTVIGNTVEIVKHTRNEDVDIESIP
                     LDDPMVYEMLRNAESEGVFQLESSLFQNMLREVKPTKFEDLVAIVALGRPGPMVMTGD
                     FVRGKHCHDTVKYPHPALEKILEPTYGVMLYQEQVMQAASELAGFSLGEADMLRRAMG
                     KKKPEVIAGLRDRFMEGALGRGVSERAAQEVFSLIERFAGYGFNKSHSAAYALISYRT
                     AYLRCHYFPEFMASTLTSVMGSSERVAMYIDVCRAAGVDVLPPNVNESFKGFTVSGNT
                     IRFGLGAIKNVGEGAAESIISARKSGGPFVSFVDFCNRVDMTAVNRKALESLIRCGAF
                     SEFGKRRALLAIMDQVCDQSAVRQRHQESGQASFFDLFEEPSGFGTADIPLPDVPDFS
                     ESQILAMEKELLGLYISGHPLASVAEAIRKVATMSVRDLSNAEDGAYATLAGVIIGRK
                     QIITRTGQPMAFVQIEDLTGQVEVVVFPRTYQECADILARDAIVVVKGRVDVKEEGIK
                     ILADSITELPKILANA"
     gene            7686..7889
                     /locus_tag="PLU15_09360"
     CDS             7686..7889
                     /locus_tag="PLU15_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOO31165.1"
                     /translation="MWTVVHIAPNRPEAELVKNMLEGEGLLVMLRPIGVPHMGDSANV
                     EVMVPETEAEEACAILDASDRLL"
     gene            7923..8927
                     /gene="pfkA"
                     /locus_tag="PLU15_09365"
     CDS             7923..8927
                     /gene="pfkA"
                     /locus_tag="PLU15_09365"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOO31166.1"
                     /translation="MRRIAVLTSGGDAPGMNPCIRAVVRKSLYHGLEVVGVRRGFHGL
                     VTGDFMEMKSRMVGDILQRGGTILKSARSEEFKTEEGRAKALLQIRTCEIDAVVGIGG
                     DGTFKGLHLLSEMGVPTVGIPATIDNDVGSTEYAIGFDTACNTVLDAINKIRDTATSH
                     ERTYVIEVMGRDSGHIALATGLAGGAESILIPEIEYDIQQVCTRIMDGARQGKSHSIV
                     IVAEGAGGEPPVSTGGAFQEGAAYRIGHQIANITGFETRIIVLGHIQRGGAPSVRDRI
                     LATMFGAKAVDLLLAGEDNAVVGMCANQVRAIPFDEALAMKRPINMDYYELANILSNL
                     "
     gene            9047..9580
                     /locus_tag="PLU15_09370"
     CDS             9047..9580
                     /locus_tag="PLU15_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HOO31167.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGEV
                     ILKKYSPMTDLSFFAQEYADSLYDATGCIAVIADEHEVVAVSGAPKKLFFEKRISEDA
                     ESIMKSGETFVTSTPGEKPVIDGAQFACQVVAPIFAENEPAGVVVLMSKEPREMGTTE
                     TKLVETAAAFLGKQLLH"
BASE COUNT         2305 a         2321 c         3045 g         2300 t
ORIGIN      
        1 ataggcattg gtgtatgtcc ggggaagaga acaacagtca gttggacgcc ggggttcggg
       61 agccttgagg tggaaatcgg ctttcagccc tatccaatat cgcctaagga tgttgccgtt
      121 agcatagagg acacctctgc cacggtttcg tggactccag tagaagagag catggtcgac
      181 cactacaggg tctactggcg ttattccact caggagcggt acattacgtc cagatctgtc
      241 gtggttgaag cgccggcatc atcggccgca gtgtcgctct cttcgcgtca acgcggctca
      301 aaagtgaggt tcgtagttgt tgcgcgggat gcgcaagggc aggagagcct ccgaagcgat
      361 gaggcggttg cggaatacag gtgatggtat cggtaacaag gccgcccgca tggcgggcgg
      421 cgcacctata ctctcgttta tttctcgagc tctttcagag ctgcagcact gatttggcta
      481 tgctgaagta gatcagcagg ccgaaagcgt ccacgatcgt ggttatcagc ggcgccgaca
      541 tcactgccgg gtccaccttg agggatgtag ctattatcgg cagaagtgcg cctatcacgg
      601 tgctcagaac gactattgct agcaccgcca ggcaaacgat aatgccaata tccgatgagc
      661 ctccaacccg ccaagcccga aagaatccta cgccagccat gactaccccg aggccgagtc
      721 cgacgacaag ttcccttgcc atcacagtcc atacgtcccg aagtcgtata tcccttaccg
      781 caaggcctcg tatgaccacg gtggatgcct gagagcctgc atttccgccg cagtctatca
      841 gaagcggtat gaagaaggtt agctctataa cgctttgcat cgcgtcactg aactgccgca
      901 ttatgcctcc ggtaaaagac tcggcgatga agagtaccag gagccatacg atacggctgc
      961 cccaaagctc tttgatgctc gtcgagaaat acgggcgttc cagaggcatt gttcctgcga
     1021 ttttctgcat gtcttctgtg gcctcttcct cgaaaacgtc gagaatatcg tcggctgtaa
     1081 tgatgcccgt gagtctttgt tcgttgtcga cgacgggaag agccagaaat cgatacttct
     1141 tgaacagttg ggcgacgtct tcctggtccg cctcgactgg cactgaaagc acgttagtgt
     1201 tcatgatggc gtcgatccta gtggaaaggt ctgccacgac caggtccctg agggacaaga
     1261 ctccgaccaa atgttcgtcg tcatcgagca cgtaaatgta gtagactgtt tcggcctcag
     1321 gagctacgcg tcgaagctct gccaatgtct gctccacggt ccagtcggac tggatggaca
     1381 cgtattctgt ggccatgatg ccgcctgagg agtgctcatc atattgaagc agttcccgca
     1441 catcttgtcc gtgtgagttc atgagatcca aaagggtttc agccttggct tccggaagtt
     1501 ctgcgattag gtcggcggcg tcgtcagagt ccatctcctt tagtatctca gaggaacggg
     1561 ctgcgcccat tagcctgatc agctctgctt gatctgcagg ctccagctct tgaactatgt
     1621 cggctgcgac atccgcggga ataacgtgga agaatgcgct gcgttccgca tcgtcaagtt
     1681 cttcgagcag gtcaactatg tcggctgggt gcagtgtagt cgcgaattca atgagggcct
     1741 cgtcggttcc agttgccagc agagccctca tatgttcgat gatgttctca taggtttcat
     1801 ccacaataac accccctatg tggggcgctc accaaccttt ggaagaggtg gacgacgccc
     1861 cagattcgat tgcatgttgg atgaatgacc gctggacaga gctggattta ttcatgtgct
     1921 ctgtactgcc ggggtctccg tccacttatt ctccctcctt ttggcgatca actggaaacc
     1981 tgatgaccca gtgcaacagc gtgtcagttg ccttggtaat tatactattg ctgcagcaga
     2041 ttgtcactct tggcgttgtc ttgggcctcc aatctctgca caaaatcgag gtcctgcggg
     2101 gacgacgtgc gttttgctta gcttctttcg cctaagtaac ttgcgagaac atcttggtcc
     2161 atttgagctt gaactgtggg cggtacaagg ccgataaact cgcaggaact tactgttacg
     2221 tgattggctt ggcgggactt ttgggtgcga tggaattaac ccggggaaga tatgcaaaca
     2281 ctatcctggc ttagcggggc actaacccga atgggccgaa aacggcgcga ctctacaggg
     2341 gtcaagtcga gggcccgggc tctgactgat cgacccacat taggtcatgg cgcgccgagt
     2401 aatcacacaa aagcaattta gcgctttcaa gcggccaaga agctattcaa atgtagagat
     2461 ggcatggaag aataatcaga aactgcttgg agttggtggc tagggtatat ggtctcgtgg
     2521 ttgaaaaccc acaaggatga gcgcgacgga gcgttagatc tgtaagtttg agcactttgc
     2581 agggacatgc cttatagggc cataatgcgc tggggagacg caacgggctg tgcaggaaga
     2641 gcaagcaagt cacgcagaga atatctgatg acaagtctcg acgagttcct gcacggtcct
     2701 cggggccatt cgcgtgcact gggggttacc gggcgcgtca cttccgtcgt tttgtgtacg
     2761 gtatgaagaa gtcgcaaatg tcggcagata tgataaaatc aagatgtgca cggcagtaag
     2821 ccgaatggat gtggggcgtg ctcggtgatt cacagggctg ggcgttccct gcaagtgtgg
     2881 gggtgtaccg atgtctgatt tgcgggaggc ggcgctgatt gcggtcaggg actgcatggg
     2941 cgcaaggtct ggcgaggatg tgttggtgat catggacaag ggtacgcgta cagtgggaat
     3001 ggcgcttttt gaggccgctg tatcccttgg gtgcgaagct atggttgtgg agataatacc
     3061 gcgcaagatg aacggtgagg agcctcccga gccggtaggc gagatgatga agcacgttga
     3121 tatcgtgctt gctcccacca gcaagtccat tactcacaca gtggccaggg gaaatgcgtg
     3181 caaggcaggt gccagagtcg ctactttgcc gggcattacc gaggactgta tgactcgtgc
     3241 gctcgtggct gactatgatg aggtggccaa gagggctgaa aaatatgccc ggatattgac
     3301 ggatggcaag atagttcgta taaccaatgc tgatggaacc gatatatcaa tgtcgctgga
     3361 aggcagagtt gggatggctg ataccggcat ctatcgagac cccggacaat caggtaacct
     3421 tccagctggc gaggccttca tcgctccggt tgagggaacg gcaaatggag tattcgtggc
     3481 ggatggggcc atggcggggc cggagtttgc aggcacgaaa atcagaataa ctgtggctaa
     3541 tgggtatgct acgagcattg aaggcggaga ggctgccagg accatagaga agatgataag
     3601 cccgctgggg atgccggcgc ggaatattgc ggaacttggg ataggcgtaa atgaccgtgc
     3661 gaagataaca ggccaggttt tggaggacga aaaagtcatg ggcacaatac acattgctct
     3721 gggcgacaac tccaatttcg gaggaaatgt gagagtcgca agccacctgg atgggatagt
     3781 tgtggcgccc acggtggaag tggatggtgt agtcataatg gattccggga agtttaccca
     3841 ctgaaggact cgagcgaaca ctctatgccg ctcgtttgcc gatgggcgga aatgcggagg
     3901 ggatgcaggt gagaaaggtc atccataaga tcccgaggca gattcgagtt gcaggattca
     3961 gtggcgacag gaagcggcgt ccacccgatt ttacagacag cgatgttgta gatgcgattc
     4021 tgactgaggt tgaatgggag gaggaaggat tgcccgacac gcggctggac gggtggtggt
     4081 ccgatgcgtg gtctcagccg gctggaaaac ctgaagaccg ttgacttgcg gctttcctat
     4141 cttatgggga gccggagcat tcaggattct gtgccaatgg ttgctcaggc ccggatggag
     4201 ctttggcgga ggcagccaag ctgagcaagg cactaactga ctcatcgggc tgatgtggca
     4261 ggagaagggg tggggacttc cgcccttttg cgggtggggg ttcccgccta ttcgcatgtt
     4321 ttttggctag ggggtggttg tgtgggggag ttcgttcatc ttcatcttca tactcagtac
     4381 tccctcctgt gattggatgg cgcttgtaga ccggacgaac ttgccagcag gatttcggag
     4441 ttgggaatgg attcatgtgc aataactgat catggatgcc tgtacgggct cgtggatttc
     4501 tatcgggctt gtaatcagca aggggtgaag ccgattcttg gctgtgaggt ctatgtggcc
     4561 aatcgcacct tgcatgacag aaccccgcat atagatgaca atccttatca tctggtgctc
     4621 ctggcagaga acgatgtggg ctactcaaac ctgctgaagc tgtcttccct cggatacacc
     4681 gaaggcttct actacaagcc acgggtggat aagaaagccc tggctgagca ttcagaaggc
     4741 ctgattgccc tttcagcttg tctgtcgggc gaagtcccgt catacgtggc gcaagggagg
     4801 attgatgaag cagaggcggt tgcaaggcag tatctagaga tattcgggcg cgattcattc
     4861 ttcctggaga tccagtctaa tgggatgcgc gttcaggatt tagtcaatcg tcagttagtg
     4921 caattgggca ggaagttggg cgtgccggtt gtagccacca atgacgtgca ctatttgcgg
     4981 cgtgaggacg ctacagccca tgatgtgctc ctttgcatac agaccggctc agtagtggat
     5041 gacccgtcgc gcatgaggtt tccgactgac caattctatc tcaaatccgc ggaggagatg
     5101 tacagggagt ttgcagagat ccctgaggca tgcgcgatga cggcgcgcat agcagaacgc
     5161 tgcaatgttg agttggattt tgatacgttg cacatgcctg agttcgaggc ccccccgggg
     5221 cagactctcg acagttactt gaggagactg tgcgaggagg gagcgataag gcgtttcggc
     5281 gccatatccc gcgaggttcg ggagaggctc gactacgaat tggggataat cgagcgcatg
     5341 ggctactcgg gctatttctt gattgtgtgg gacttcatac gctatgccaa gagccaaggc
     5401 atatatgtag gaccaggcag aggttcagct ccgggcagcc tggcagctta ctgcctgggg
     5461 attacagatg tggatccctt ggcatacaac ctcctttttg agaggtttct caatcctgaa
     5521 cgggtgacaa tgcccgatat tgacgtagac ttttgctatg aacgtcgcgg agaagtcatt
     5581 gattacgtgg cagggaaata cggttctgac aaggtggccc agattataac tttcgggact
     5641 atggcggcgc gcgccgccat cagggacgtt ggtcgggccc tcaagttcag ctacgcagag
     5701 gttgaccgca ttgccaaact cgttcctgcc gagctcaaca tgacgattga tcgggcactt
     5761 gaagtctcgc cggagcttgc tgctgcagtg ggagaaagcg aacgcaatcg aacgttgata
     5821 gagattgccc gcgctgtgga gggtctgcca cgccaccctt cggttcatgc agcaggggtt
     5881 gttatctcaa aagatccgct cacagatcat gttccgctat ataaatccgc tgacggcgtt
     5941 ctgacgacgc agtatcccat ggaggacctt gaacgccttg gcatagtcaa gatggatttt
     6001 ctcgggctgc gaacactcac ggtgatcggg aataccgtgg aaattgttaa gcacacccgc
     6061 aatgaggacg tggacatcga gagtattccc ctcgatgatc ctatggttta tgagatgctg
     6121 aggaacgcgg aatcggaagg cgtgttccag ctggaaagca gcttgtttca gaacatgctt
     6181 cgagaggtga agcctacgaa attcgaggac ctcgtcgcca ttgttgcctt gggccgtccg
     6241 ggtccaatgg tgatgacagg ggatttcgtc cgcggcaagc attgccatga cacggtcaag
     6301 tatccccacc cggcactgga gaaaatactc gagcccacat acggtgtgat gctctatcag
     6361 gaacaggtta tgcaggcagc gagtgagctt gccgggttca gcctcgggga agccgacatg
     6421 ctcaggcgcg ccatgggcaa gaagaagcca gaggttattg cgggtttgcg ggacagattc
     6481 atggaagggg ctttaggacg gggcgtgtcg gaacgcgcgg cacaggaggt tttctcactg
     6541 attgaaagat ttgcgggata cggtttcaac aaatctcata gtgctgcata tgccctaata
     6601 tcctacagaa ccgcctattt gaggtgtcac tactttcccg aattcatggc ctctacacta
     6661 actagtgtga tggggtcgtc ggaacgcgtg gccatgtata tagacgtgtg cagagccgca
     6721 ggtgtggacg tgcttccgcc taatgtgaat gagagcttta agggatttac cgtctcgggc
     6781 aatacgatac ggttcggcct tggtgctatc aagaatgtgg gcgagggcgc cgctgaatct
     6841 atcatatctg ccagaaagag cggcggtccg tttgtgtcat ttgtggactt ctgcaatagg
     6901 gttgatatga cggcagtcaa cagaaaggct ctggagagcc tgataagatg tggcgcattc
     6961 agcgaattcg gaaagaggcg cgcccttctt gccattatgg accaggtatg tgaccagagt
     7021 gcggttcgcc agcgccatca agagtcaggg caggcgtcct tcttcgacct gtttgaagag
     7081 ccttccggtt ttggcactgc cgacattccc ctgccggatg tacctgattt cagtgaatca
     7141 cagatactgg caatggagaa ggaattgctt ggcctctata tctcaggtca tccgttggct
     7201 tcggttgcag aggccatccg caaagtggcc accatgtcag ttcgcgatct gtcaaacgcg
     7261 gaggacggcg cgtatgcgac gcttgccggg gtgatcatcg gacgaaagca gataattacg
     7321 cggacagggc agcccatggc ttttgtgcag atagaagact tgactggcca agtggaggta
     7381 gtggtttttc ccagaactta tcaggaatgc gcagatattc ttgcccgcga tgccatcgtc
     7441 gtggtaaaag gccgtgtgga cgtcaaagag gaaggaatca agattttggc cgattccatc
     7501 accgaacttc cgaaaatact cgccaacgcc tgaaatcttt gataccatac ttggatggga
     7561 gcggttgcgc tgggcaggac tgcatgacaa caggctacat gccggactgt gagaaggaat
     7621 cgctgaagat ggatagaaga acggacatac taaagcggaa gattcagcga gggggattaa
     7681 acgtgatgtg gacggtagtg catatcgcgc ctaataggcc agaggcggaa ctcgtgaaga
     7741 atatgcttga aggagaaggc ttgttggtga tgttgaggcc gattggagtc ccccatatgg
     7801 gcgattcggc gaacgtggaa gtgatggttc cagaaactga agctgaagag gcctgtgcta
     7861 ttctggacgc ttccgataga ctactttgat gacagtgggc ggggacgatt gggggaatta
     7921 tcatgagacg cattgcagtg ctgaccagtg gaggcgacgc gcccggcatg aatccatgca
     7981 ttcgtgctgt tgtgcgaaaa tcactctatc acgggcttga agtggtcggt gtcagacgcg
     8041 gctttcacgg actggtcaca ggcgacttca tggaaatgaa atcacggatg gtaggggata
     8101 tactgcaacg cggtgggact attctgaaat cagctcgttc ggaagaattc aagactgaag
     8161 agggtcgcgc caaggcgctc ttgcagataa ggacgtgcga gatcgacgca gttgttggaa
     8221 tcggcggtga cggcaccttc aagggtttgc atttgctctc tgaaatgggc gttccaacag
     8281 tgggcattcc agctactatc gacaacgatg tcggatcaac tgaatacgcc atcgggtttg
     8341 atacggcatg caacactgtt ctggatgcga taaacaagat acgcgacacg gcgacttcac
     8401 atgaacgcac atacgtaata gaagtcatgg gaagggactc aggccatatt gccttggcga
     8461 ctgggcttgc cgggggtgcg gagagtattc ttattccaga gatcgaatac gacatacagc
     8521 aagtgtgcac aaggataatg gacggcgccc gccaagggaa aagccacagc atagtgattg
     8581 tggcagaagg ggccgggggc gaaccccccg tctcaaccgg cggagctttt caagagggcg
     8641 ctgcatacag gatcggacac cagattgcca acataacggg gtttgagacc agaatcatcg
     8701 tgcttggaca tattcagcgg ggcggtgctc catcggtgcg tgaccgcata ctggccacga
     8761 tgtttggcgc caaggcggtg gacttgcttt tggcgggtga ggacaatgca gttgttggca
     8821 tgtgcgcaaa ccaggttcgt gcgattccct ttgatgaagc cctggcaatg aagcgcccga
     8881 tcaacatgga ttattatgag ttggccaata tcctttcgaa tttgtgatga cacgcgggtg
     8941 cgcaacgtgg catgattggt gctgagtata ggttgtagta tacgttatca acaataaagg
     9001 tacaatagag cctgaaatgg cggaaatcag ttggggaggg catgaagtga aggcaacagg
     9061 catagttagg cgcatcgatg acctcgggcg ggttgtaata ccgaaggaaa tacgaagaac
     9121 gctgaggatc cgcgagggcg atccacttga gatatttact gatcgtgacg gggaagtcat
     9181 tctgaagaag tactctccca tgaccgactt gagtttcttt gcacaggagt acgctgattc
     9241 tctctatgat gccacgggct gcattgcggt gattgcagat gagcacgagg tcgtagcggt
     9301 gagtggagct ccgaagaagc tcttcttcga gaagaggatt tctgaagatg cggaaagcat
     9361 catgaagtcc ggggagactt tcgtgacaag cacgccgggt gagaagcccg taattgatgg
     9421 ggcgcaattt gcgtgccagg ttgtggctcc gatatttgcc gaaaacgaac ccgccggagt
     9481 cgttgttcta atgtcgaagg aacccagaga gatggggacg acagagacaa aactagtgga
     9541 aactgcagcg gcgttcctgg gcaagcagtt gcttcattag tctgtgtgcg catacagcca
     9601 cctcgccgcg ggagatgtca gacatacggg ctctaggcct ggaggctgat acacaccgga
     9661 tagctggcga ttttgcattg cataggtaat actgagagtg aagacccctt cgggatgctc
     9721 ctgtgggggt tttcttgcgc cttttattgc acagagccac cttggtgttc ttgacttgtc
     9781 tggtgtttct gcacatgaag gtgtgactgg ccccggcctt tgtttggtga caagtgcgcg
     9841 aacatgcgga ggttcgatgc cagtgtcaga tgaccgtctc ctcgctatgg ttgtgcaggt
     9901 ggtatggccc atccagcgag gaggtggcct atgcgcagct cattgaggag attgcactag
     9961 aggattacat c
//
LOCUS       DAOSYL010000071         9947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5786_length_9947_cov_7.794986, whole genome shotgun sequence.
ACCESSION   DAOSYL010000071 DAOSYL010000000
VERSION     DAOSYL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5786_length_9947_cov_7.794986"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1122)
                     /locus_tag="PLU15_09375"
     CDS             complement(295..1122)
                     /locus_tag="PLU15_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31168.1"
                     /translation="MISNMQPGSVADYIKSGYALVQRNYNLIKHEMGWEVVNAIYQVV
                     SALTIGFIGVSGPPEGMNERVLFLVSGALLWGFLSILFHEVAESVAWERWEGTIEYSF
                     MAPQPRGVYMFGVCMWGMLYGLVRVVICLTAVTLAFNISLAGANLGAAFVTLAASSLA
                     FIGMGLAAAVLPLISPEKGSQATHIFQAVVLLVSGVYYDIEVLPAWVRPLSYVSPATY
                     TLRAARAALLEGASVRTLLPDLLKLVILGAILIPVGFRIFSMGENYAMRTGKLKRTG"
     gene            complement(1112..1864)
                     /locus_tag="PLU15_09380"
     CDS             complement(1112..1864)
                     /locus_tag="PLU15_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31169.1"
                     /translation="MPSRVTVEAVKDVSFEVERGEIFGILGPNGSGKSTLIRMISTLL
                     IPDSGTVRVFGRDVVADRLEVRRYINRVSVEAAFFKKLSAMENLEYAARLYGLEPREG
                     LRRAREILARLGFPDSKVNVSLENLSRGMQQKVAIARALMTAPVALLLDEPTTGLDPV
                     SKRDVQDFVLEVCNTHDTTVILTTHDMEEADRLCDRIAVINNGEFVAVDSPENLKDRY
                     ANGNGHLPTLEEVFFRLTGRSPDEWEIEGDDQ"
     gene            complement(2399..3568)
                     /locus_tag="PLU15_09385"
     CDS             complement(2399..3568)
                     /locus_tag="PLU15_09385"
                     /inference="COORDINATES: protein motif:HMM:NF017328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HOO31170.1"
                     /translation="MSLRRMSALVCAVILVAFLMPFASGCWDSIDIEDRSFVAVAALE
                     PAEGGENKGVSVSILIPIPGGVQGGANGGGRGNGQDNAQKSVVIKENGLYIEDAVDKM
                     RAKFGGVIDFSSLQFIAIGKDVDPEDMWKGLSTVLFFRYVPLTSLIFATEDDMGELLR
                     LSPASGKRLVEVLNGLETQSKQGLGSVRFLSVWQVMSLIRNDVGDVVLPLIGLTDGGD
                     GIEALGTAVYDGTRRAGTLGPTDSMLLYGIAWRKHLGGTYTMHYEGREFIVRIIRMKT
                     KTRMTGVDANGLPRVGVKLSVAARLLDSGGYRVSLVDRSRLNDLERAAERDLEDRLER
                     ILMKLKAWNSDAMRLYYALRLKTPYMSYDDFKGVYSKMEVDFAVSMSLRRAGLLK"
     gene            complement(3569..4687)
                     /locus_tag="PLU15_09390"
     CDS             complement(3569..4687)
                     /locus_tag="PLU15_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOO31171.1"
                     /translation="MREAYLSWPEAAWLVMQYTICANYLALPQMLYHMAGRSGWVVPF
                     IAIAPAMAGSWAVYRLHMAYPKHRVGQFLPQLAGRHLGVVIGLIYSLFWLSGAAINTM
                     LFAHYISATILGFTPPAIHAIINSGLAVMVALYGLKTLVRFTDALFLPIVVLLIFSWI
                     SPFLGDKLDLSHLLPLRASELAADPFGLMIAATSMYHGYTVILIAGPAFNPPGRSALL
                     AATVGTLLAVPGFVTFSAYPIAAFGWPLAGDFAYPAGSFLEVLAPGSQGFPVRRFDFI
                     LTMFFRFIMVIASITYFFSAAQTLSDLVSPKAEKIHPVLVLALGALIGFSFVVLPDLS
                     SLLAFVRIWLALGAILTIVTIILAVRSYLATTRAGGDE"
     gene            complement(4680..6194)
                     /locus_tag="PLU15_09395"
     CDS             complement(4680..6194)
                     /locus_tag="PLU15_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOO31172.1"
                     /translation="MKKPVPLLSRPVRRPTEPSVSKRDSSSSSMVQSQAACLENALGV
                     PSDLVIKHVSDSICVVFLEPIVDGAAVRESLLKPLSKLESDEILDAVSVGAKLPVASV
                     EQAPSLDDAVEGLLDGKVALIVDGCVAPFLLDLRGRVVRQIEAPVSQPSVRGPRDSFN
                     ERLYDNLALIRQRVRNRNLRVWETVVGSETRTRVAMCYLEGKASEDVVEVVKQRLLEI
                     DAPNVLDSSYLLPYLAMRRWMLFPPAAATERADRAAAGILNGRIVVICDNSPFVLVMP
                     IQLLALFQASEDHYNLSIHSLLLRTVRAIGWFAATIAPGVYVGLASFNPGILPPNAIM
                     TLSAARQGVPYPPIIEVLIMDIALEMLAEASARLPTYIGGAAMVVGGLVLGTAAAEAR
                     LVSTVMIVVVAVTAIGSFAMPDYQNELSWRAAKYLYTVFAAFLGIYGLATAGFIILVY
                     LCSMDVLGVSYMSPFAPWRWTAIAKDTMVRVPTPLGIELEKVQRKEEAEGTADA"
     gene            complement(6313..7635)
                     /locus_tag="PLU15_09400"
     CDS             complement(6313..7635)
                     /locus_tag="PLU15_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO31173.1"
                     /translation="MELAKKSSLKILMVEKGRDIGERTCPALERKTSCARCDPCNVVC
                     GWGGAGAFSDGKLTLSTDVGGMLEAYVGSAGLKKLIEEVDRVYLEHGAPETVWGLEEE
                     KIADLRDRAKLADLTFVPARIRHLGTGRTVNILERMKDGLDGRVDIELDTSVNSVVAS
                     EGVVRGVETSDGRFISGKCVILAPGREGAEWLADQARHLGLRMAINPVDIGVRVELPA
                     VVMKDITDVVYESKFIYYSKSFDDKVRTFCMCPYGEVVTENNDGLITVNGHSYAERKT
                     ENTNFALLVSKSFTEPFREPIQYGKYVAGLANLLAGGVLVQRLGDLMVGRRTTEERLM
                     KGTVTPTLKDATSGDLSLVFPYRHLVDIMEMLQALDKVAPGVYSRNTLLYGVEAKFYS
                     NRLELSRSLETEIGNLFAAGDGAGVTRGLMQASASGVVAAREILSRFE"
     gene            complement(7781..>9947)
                     /locus_tag="PLU15_09405"
     CDS             complement(7781..>9947)
                     /locus_tag="PLU15_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO31174.1"
                     /translation="SYVEYWAYGVPQNRVGTSMLATSHSVTLSNLRAGTTYYYRVIST
                     DAAGNRGQSQDLTLVIQPVDTTPPVISRIEAKATGLDSVSITWTTDEPADSYVEYWAY
                     GVPQNRVGTSVLSTYHAVTLSNLRSGVTYYYTVTSTDAAGNRARSSEMSLRIQPPDTT
                     PPTVRVQINGGAQYTKSRTVQLEITAQDDSGGPLEMRIWDDKSSYGSWRAYSDRVTWE
                     LTTGDGRRTVYVKVRDAAGNEAQAQAVITLDTEPPIIRSVQTKNIGPDSAVITWSTDE
                     NSDSWVYYGTYSPNLVMGQYDFVTSHSVALYGLSPNTDYYFRVRSADAAGNMAESSTL
                     TFRTSQLGDRNPPTGSIAINNGAQYTRYYDVTLSIAAKDDSPGPIEMRLREGSGNWTG
                     WMQFQQAMTYRLSPGDGNKTVYVEFRDIYGNQSRTYTASITLDTRPPKITNVRTANIT
                     ANSATITWNTDEPATSTIEYGLTGTTMKDAAGDKSVSSSEASSPAGDVGARSVIITPV
                     VPSGSLKTSHSVTLTGLRDNTTYYYQVVSKDAAGNVSVASGFSFTTGSVAPPPPIGTV
                     NVNWAAAANGGRASASSYAPASSDGPARPASMAIDGNMKTYWEASGPITGGKTEWLEV
                     EFSASRVINTIRTSSDVGNYPYSMTVEVEENGRWIVVGSFTKPSEYQKYMKRSGDVVS
                     FEFQFTAMSVSKVRFVVARVSNPLNALQFYEVEAFNTGR"
BASE COUNT         2353 a         2947 c         2428 g         2219 t
ORIGIN      
        1 gaatcatatc ccggcacggc tgagtgctgc gggcgtttat ggctaaggcc aagatccccc
       61 caagactgtg tacgttgtcg atgtacaaat gcgaaccaat taccgcactg cgacataggt
      121 gcaaagacgt gtctacgcct agcagacatc atatggcagg ctaaccgata gcgcaagaac
      181 ttattgggag ttacttagct gctcgaaaag cgagggcacg gacgtcacgg cgatttgcgt
      241 gccgtcccgt ccgcgccttt tgctgtcctg tgcataggct gctgcaatgg gagcttatcc
      301 cgttcttttc aacttgcccg tgcgcatcgc atagttttcg cccatggaga agatccgaaa
      361 cccgactgga ataaggatcg cccccaggat cacgagtttg agaaggtccg gcagaagcgt
      421 cctcacacta gcgccttcga gcagagcagc tctggccgca cgcaatgtgt aggttgcagg
      481 agatacatac gacaacggcc tgacccatgc aggcaaaacc tctatgtcgt aatatactcc
      541 cgacactaac agcaccacgg cctgaaagat gtgagtcgcc tgtgagccct tttccgggga
      601 tatcagaggc aacacggctg cagccagccc catccctatg aaggcaaggc tcgacgcggc
      661 aagcgtgacg aaagccgccc cgaggttagc ccctgcaagc gaaatattga acgcaagggt
      721 gaccgctgtc aggcatatca ctacgcgcac cagcccatac agcattcccc acatgcaaac
      781 gccgaacatg tacacgcccc tcggttgcgg ggccatgaac gaatactcga tggtgccttc
      841 ccacctctcc caggcgactg actctgcaac ctcgtgaaaa aggatggaaa gaaagcccca
      901 cagcaacgcg cccgacacga ggaagagcac ccgttcattc attccctcgg gaggcccgct
      961 cacaccgatg aatcctatcg taagggcgct cacaacctgg tagatggcat taacaacctc
     1021 ccagcccatc tcgtgcttga tcaggttgta gttccgctgc accaatgcat agccggattt
     1081 tatgtagtca gccacagatc caggctgcat attactgatc atctccctct atctcccatt
     1141 catctggact ccttccggtc aatcgaaaga aaacctcctc cagagtggga agatgtccgt
     1201 ttccattggc atatctatcc ttcaggtttt ccgggctgtc caccgcaacg aactccccgt
     1261 tgttgatgac ggctattcgg tcacacaacc tatcagcctc ctccatgtcg tgggtggtga
     1321 gtatgaccgt tgtgtcatgg gtattgcaga cctcgagcac gaaatcctga acgtcccgtt
     1381 ttgacaccgg atcgagccct gtcgtgggtt catcgagaag caatgccacg ggagcagtca
     1441 tcaatgctct agctatagcc actttctgct gcatcccgcg ggacagattc tccaacgaca
     1501 cattgacctt gctgtcggga aaccccaacc gcgctaggat ctctctggcc ctgcgcaacc
     1561 cttccctagg ttcgagccca tagagccttg cggcatattc gagattctcc atggcggaca
     1621 gcttcttgaa aaatgccgct tctaccgaaa cgcgattgat gtaccggcgc acttcgagtc
     1681 tgtctgcaac aacatccctg ccgaaaacgc ggactgttcc ggaatcgggt atgagcaatg
     1741 tcgatatcat ccggatgaga gtggattttc ctgaaccgtt gggccctagg atgccaaata
     1801 tctcgccgcg ttcaacctcg aaggacacgt ccttcactgc ctcaacagtc accctggaag
     1861 gcatgacaaa gtcaacgatc cgcccaagcg ccctctggcc attatggtct gaacctgcgt
     1921 tttcagtatt gcgcacggat tccctgaagc tcttggatgc gtgattgcac tgaatcgctg
     1981 cagtcacgtc aataacctcc ttaagatgct gactcgacta caggatagtt aggctgggcg
     2041 ccaattggct gcaagcttcg cggccaagcg caatgcaggc taaacgttaa aaggccgcgg
     2101 gctttgcacg cccgcggcct gtaaatgcgt caatagcgca ttatcctacc ctcagacgag
     2161 ccgacccgat ataaacagaa ttaggcatag taacccatga aacaagacga actccgtttc
     2221 ccgaacacgc ggtgatgatc tgcctgattt tcgtcatcgg ttcggcctcc tttctgatct
     2281 cacggttggg ataatcaaac atggtaagta taacgtggaa gggatatcgg cgtcaagcga
     2341 aaaatccact cgcataacgc atgtccattg ctctgctcag gcacggcgtt tccgccgctc
     2401 acttgagcaa ccctgcgcga cgcaagctca tcgaaacggc aaaatcgacc tccatctttg
     2461 aatatacgcc cttgaaatcg tcatatgaca tatacggcgt cttcagcctc agtgcatagt
     2521 ataacctcat agcatcggag ttccacgctt tgagcttcat cagaattctc tccagcctgt
     2581 cctctagatc gcgctcagcg gcgcgctcaa gatcgttcaa tcggctccga tcgaccagcg
     2641 aaactcggta gcccccagaa tcgaggagcc gagcggcaac agacagcttt acgccgacgc
     2701 ggggcaaccc attcgcatcg acgcctgtca tgcgggtttt ggtcttcatg cgtatgatcc
     2761 tcacgatgaa ttcccgtccc tcgtagtgca tggtgtatgt gccacccaag tgtttgcgcc
     2821 aggctatgcc gtatagcagc atcgaatctg taggcccaag cgttcctgct cgccttgttc
     2881 cgtcatatac agctgtccct agggcctcaa ttccatcgcc tccatcggtg aggccgatca
     2941 acggcaagac aacatcgcca acgtcatttc tgatcaagga catcacctgc catacggaga
     3001 ggaaacgaac actccccaac ccctgcttgc tctgtgtctc taagccattg agcacttcca
     3061 cgagtctctt tccgcttgca ggagataaac gaaggagttc gcccatgtcg tcttctgttg
     3121 caaatatcaa gctggtcagc ggcacgtatc gaaagaagag aaccgtgctc agccccttcc
     3181 acatgtcttc aggatctacg tctttcccta tggcaatgaa ctgcaaggat gaaaagtcta
     3241 tgacccctcc gaatttggcg cgcatcttgt ctactgcatc ctctatatac agcccgttct
     3301 ccttgatcac cacgcttttt tgggcgttat cctgtccgtt gcccctcccg cctccattag
     3361 caccgccttg cactccccct ggaattggga tcagaatact gacagaaaca cctttgtttt
     3421 cgccaccttc agcaggttcc aaagcggcaa ctgcaacgaa actgcggtct tcaatgtcaa
     3481 tcgagtccca gcatccggat gcaaaaggca ttaaaaaggc aacaaggatg actgcacata
     3541 cgagtgcaga cattctgcgt aagctcacct actcatcacc gccagcacgc gtcgtagcga
     3601 gataagaccg aacggccaag ataatcgtga caatagtaag aatggctccc agcgcaagcc
     3661 aaatgcgcac aaacgccaag agagaactga gatctggcaa tacaacaaag gaaaagccta
     3721 tcaacgcccc caaagctagc actagtacag gatgaatctt ctccgctttg ggagacacca
     3781 agtcgctcaa tgtctgtgcc gcgctgaaga aataggtaat ggatgcgatg accatgatga
     3841 aacggaagaa catggttagg atgaagtcga aacgccggac tgggaatccc tggcttcctg
     3901 gcgccaggac ttccaggaac gagccggcag ggtatgcaaa atcgccggct aaaggccagc
     3961 cgaatgcggc aatgggatag gcagaaaaag ttacaaaccc cgggactgcc aggagggtgc
     4021 caaccgtggc agctaacaat gcgcttcgtc cgggcgggtt aaacgcaggc cctgcaatta
     4081 gaattacggt gtaaccatgg tacatagatg tcgcagctat cataaggcca aacggatcag
     4141 ctgccagttc cgatgcgcgc aatggcaata ggtgcgacag atcgagctta tctccgagga
     4201 acggagagat ccacgagaag atgagcaaaa caacgattgg caagaagaga gcgtcagtga
     4261 acctgaccag agtcttgagc ccatatagcg ctaccataac agcaagcccc gaattgatga
     4321 tcgcatggat tgcaggcggg gtaaacccga gtatggtagc tgatatatag tgggcaaaca
     4381 gcatggtgtt tatggctgcg ccactcaacc agaacaagga gtatatcaga cctatgacaa
     4441 ccccaagatg tctgccggca agttgcggaa gaaactgccc aacccgatgc ttaggatagg
     4501 ccatgtgcaa gcgatagact gcccacgaac cggccatagc tggagctatc gcaataaatg
     4561 gcaccaccca acctgaacgc cccgccatgt gatatagcat ctgcggcagc gcaagatagt
     4621 tggcgcagat tgtatactgc atcacaagcc acgctgcttc cggccaactg agatatgctt
     4681 cacgcatcgg cagtaccctc ggcttcttcc ttgcgctgca ccttctccag ctcaatgccg
     4741 agaggcgtgg gcaccctcac catcgtatcc ttggcgatag ccgtccacct ccagggagca
     4801 aacggagaca tgtaagatac tccaaggacg tccatagaac aaagatatac gaggatgatg
     4861 aatccggcag tcgccaatcc atagatgccg aggaaagcag cgaaaaccgt gtacaggtat
     4921 ttggctgcgc gccacgaaag ctcgttctga taatccggca tagcaaaaga gccgattgca
     4981 gtcactgcaa caacaactat catcactgtg cttacaagcc tggcttctgc agccgctgtt
     5041 cctagcacaa ggcctccaac taccatcgcc gctcctccga tatacgtggg gagcctagcg
     5101 ctggcttcgg cgagcatctc caaagcaata tccatgatca agacttctat gattggagga
     5161 tacggaacgc cttgccttgc cgcggacaga gtcattatcg cattgggagg caagattccc
     5221 gggttgaacg acgcaaggcc cacatatacc ccaggagcaa tcgtggccgc gaaccagcca
     5281 atcgcccgca cggttctgag cagcaaagaa tgtatgctca agttataatg gtcctcgctg
     5341 gcctgaaaca gcgcgagaag ctgtatgggc atgacaagaa cgaaagggga attgtcgcat
     5401 atgaccacaa ttctgccgtt gagtatgccc gccgccgccc gatccgcgcg ctcagtcgca
     5461 gccgcaggag gaaacagcat ccatcttctc atcgcaaggt acggcagcag ataggagctg
     5521 tcaagtacgt tgggggcatc aatctctaat agcctctgct ttaccacctc cacaacgtcc
     5581 tctgaagctt tgccctcaag gtagcacatg gccacccggg tccgtgtctc gctgcccact
     5641 actgtttccc aaactctcag atttcggttt ctgactcttt gcctgatcaa agcaaggttg
     5701 tcataaagcc tctcattgaa ggaatcccta ggacccctga ctgacggctg tgatacgggg
     5761 gcttctatct gtcggactac cctgcccctc agatcgagca ggaaaggagc gacgcagcca
     5821 tcaacgatga gagccacctt tccgtcgaga agaccttcta ccgcatcatc aaggctcggt
     5881 gcttgctcca cggaggcaac cggaagtttg gctcccacgg agactgcatc cagaatctca
     5941 tcgctctcta gcttgctcaa tggtttgagg aggctttctc ttactgccgc cccgtctact
     6001 atgggttcta gaaacaccac acagatacta tccgatacgt gctttattac caagtctgac
     6061 ggaacgccaa gtgcgttctc caagcaggct gcttgactct gaaccatgct gctagacgag
     6121 ctatcacgct ttgagaccga tggttcagta ggtcggcgca ccggccgtga aaggagcgga
     6181 acaggtttct tcatgtgcat tcctgccatt cgtcgctttt gcacctatta tgccccgctc
     6241 acaaaatgag atgagactgc gcacgaatac atccactctc tccatcagaa ggcccctagc
     6301 gccagaccag tcctactcga atcgcgatag aatctctctg gctgccacta cccccgatgc
     6361 tgatgcttgc atcagtccac gggtgacgcc ggccccatcc ccggctgcaa agagatttcc
     6421 gatttccgtc tcaaggctgc gcgaaagttc caggcggttc gagtaaaact tggcttctac
     6481 cccataaagc agggtgttcc gggagtaaac ccctggcgcc actttgtcca gggcctgcag
     6541 catctccatg atgtccacga gatgcctgta tggaaaaaca aggctcagat ccccgctggt
     6601 tgcatccttg agggtagggg tgactgtacc tttcatcagc ctttcctcag tggtacgcct
     6661 gccgaccatc agatccccaa gcctctgaac gaggactccc ccggcgagca agtttgcaag
     6721 gcccgctacg tattttccgt attgtattgg ctccctgaat ggctcagtaa agctcttgct
     6781 aacaagcaga gcgaaattgg tgttctccgt cttgcgctcc gcgtagctgt gcccgttgac
     6841 tgtgatcaga ccgtcgttgt tctcagtcac cacttcgcca tatgggcaca tgcagaaagt
     6901 gcgaacctta tcatcgaagg atttggagta gtatatgaac ttcgattcat acaccacatc
     6961 ggttatgtcc ttcatcacta ctgcaggcag ttcaaccctg acgcctatgt ccactggatt
     7021 gatggccatc cgaaggccga gatgccgagc ctgatcggcg agccattcgg caccttcgcg
     7081 tccgggagca aggataacgc atttgccgct tatgaaccgc ccatcggatg tctcaacacc
     7141 gcgaactacc ccctccgatg ccactaccga attcacactg gtatcgagtt cgatgtccac
     7201 tctgccgtcc agaccgtctt tcatcctttc caggatatta actgttcgtc cagtgccaag
     7261 atggcgtatt ctggcaggca caaacgtcag atcagccaat ttggccctgt cacggaggtc
     7321 cgcaatcttc tcctcttcga ggccccatac agtttccggc gccccatgct ccaggtagac
     7381 tcgatcgacc tcctctatga gcttcttcag cccagcgctg ccgacataag cctccagcat
     7441 cccgccaaca tctgtggaga gagtcagctt tccatcgctg aatgccccag ccccgcccca
     7501 gccgcagacc acgttgcacg ggtcacacct ggcgcaactc gtcttccgct ccaaagcagg
     7561 acacgtacgc tcccctatat cccgtccctt ctctaccatc aggatcttga gactcgattt
     7621 cttagccaac tccatgccgg caaatatgcc tgccgggcca gcacctacta tgatcacgtc
     7681 gtatgcggtt ctgctcaact tagcgcctcc cgtgacgctc aactgccgca cccggatcgg
     7741 atgcggcagt cgtcgccaat tatgtccaac tcggtcttcg ctagcggccc gtgttaaaag
     7801 cctccacttc gtagaactga agagcattca agggatttga taccctagca acaacgaacc
     7861 taaccttcga cacgctcata gctgtaaact gaaactcgaa tgacaccacg tctccagacc
     7921 gcttcatgta tttctggtat tcagacggtt ttgtaaatga cccaactaca atccagcgtc
     7981 cgttctcttc gacctccaca gtcatggaat acgggtagtt acctacatca ctgctcgttc
     8041 ttatggtatt gatcacccgc gaggccgaga actcaacttc gagccattct gtcttgccgc
     8101 cggtaattgg cccggaagct tcccaatatg tcttcatatt gccatcaata gccatagaag
     8161 caggtcttgc cggtccatct gagctagccg gcgcatagct cgacgcgcta gcccgccctc
     8221 cattggccgc agctgcccag ttcacgttga ccgtcccgat tggcggcggt ggcgccacag
     8281 accccgtagt aaacgagaac cctgaagcca ccgaaacgtt tcctgcagcg tccttcgata
     8341 ctacctgata gtagtatgtc gtgttatcgc gaagaccagt caatgtcacg ctatggctag
     8401 tcttaagaga ccccgaaggc acaacaggag tgattattac cgatctagcg cccacatctc
     8461 ctgcagggct cgacgcctcc gaactcgaca cggatttgtc gcctgcggca tccttcatgg
     8521 tcgtgcccgt caagccatat tcaatggttg aagtagcagg ttcatcggtg ttccatgtta
     8581 tcgtagccga atttgcagta atattcgccg tccgtacgtt agttatcttc ggaggacgag
     8641 tatccaaggt tatcgacgca gtgtatgtcc tgctttgatt cccatagatg tcgcgaaact
     8701 cgacatagac tgtcttgttc ccgtcaccag gacttagcct gtatgtcatc gcctgttgga
     8761 actgcatcca cccggtccag ttcccgctgc cctcacgcag gcgcatctcg attggacccg
     8821 gcgagtcgtc ttttgcagct atcgagagcg ttacgtcata atagcgcgta tactgagccc
     8881 cattgtttat cgcaatgctg cctgttggcg gattcctgtc gccaagctga gacgtacgga
     8941 acgtcagcgt ggaggactcc gccatatttc ctgcggcatc ggcggaacga accctgaagt
     9001 agtagtcagt gttggggctt aagccgtaca gggcgaccga atggctcgtc acgaagtcat
     9061 actggcccat aaccaagttc gggctgtatg tgccgtaata cacccaggaa tcggagttct
     9121 cgtcagtgct ccatgttata acagcagaat ccggtccgat gttctttgtc tgcaccgacc
     9181 tgattatagg gggctccgta tcgagggtta ttacagcctg cgcttgggct tcgtttcccg
     9241 ctgcatctct gaccttcaca tagacggtcc tcctgccatc gcccgtggta agctcccagg
     9301 taaccctgtc agaatacgcc ctccaggaac cataagagga cttatcatcc caaatcctca
     9361 tctcaagcgg accgcctgag tcgtcctgag cagtaatctc caactgcaca gttctgctct
     9421 ttgtgtattg cgcgcccccg tttatttgca ctctcacagt tggaggtgtc gtatcgggag
     9481 gctggatcct aagactcatc tccgatgacc gcgcacggtt gcccgctgca tcggtggaag
     9541 tcactgtgta gtagtatgtg acaccagaac gcaaattgga caatgtaacc gcatggtaag
     9601 tcgaaagcac gctcgtgccc acacggttct ggggcactcc gtatgcccag tactccacgt
     9661 aggaatctgc cggctcatcg gtggtccatg tgatgctcac cgagtcgagc cccgttgctt
     9721 ttgcctctat ccggcttatc accggcggag ttgtatctac aggctgaatc acaagcgtga
     9781 gatcctgcga ttgcccccgg tttcctgccg catcggtaga aattaccctg tagtaatacg
     9841 tcgtgcccgc acggaggttc gaaagggtta ccgagtggga tgtggcaagc atgctcgtgc
     9901 ccacacggtt ctggggcact ccgtatgccc agtactccac gtaggaa
//
LOCUS       DAOSYL010000072         9862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5850_length_9862_cov_6.143061, whole genome shotgun sequence.
ACCESSION   DAOSYL010000072 DAOSYL010000000
VERSION     DAOSYL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_9862_cov_6.143061"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..250)
                     /locus_tag="PLU15_09410"
     CDS             complement(47..250)
                     /locus_tag="PLU15_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HOO31175.1"
                     /translation="MDSRLRIRYEVGQDDEGNPRYSTRTYSGVKPTAADQDAYDVAVA
                     LSGLQVYPVVSITRVNEAELVDI"
     gene            complement(448..1635)
                     /gene="metK"
                     /locus_tag="PLU15_09415"
     CDS             complement(448..1635)
                     /gene="metK"
                     /locus_tag="PLU15_09415"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOO31176.1"
                     /translation="MTRKLFTSESVTEGHPDKICDQISDAVLDAILEKDPSARVACET
                     AVTTGLVLVMGEISTECYVDIPSIARQTLRDIGYTRAKFGFDSDTCAVLTSIDEQSPD
                     IALGVDKAYEAKQGISNQVDAIGAGDQGMMFGFACDETDTLMPLPIHLAHGLTKRLAE
                     VRKSYILPYLRPDGKSQVTVEYEGDKPVRVDTIVLSSQHHPKVDSRIIREDLIEHVVK
                     KVVPAELIDKNTKYYINPTGRFVVGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKD
                     PTKVDRSASYAARYVAKNIVASGIASKCEVQLAYAIGVANPVSVMVDTFGTAKIPEEK
                     IIRLIYDNFDLRPAAIIRDLDLRRPIYRNVAAYGHFGRKDLDLSWERTDKAEDLRVQA
                     FGR"
     regulatory      complement(1730..1838)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1892..2374)
                     /locus_tag="PLU15_09420"
     CDS             complement(1892..2374)
                     /locus_tag="PLU15_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOO31177.1"
                     /translation="MTGVVTDVLQFIGDELAVVFVAMLPYVEIRGAIPMGISMGMHPM
                     HAFVLALAGSILPSPVIMLVFRPFFNFVKRTGLFRSLTEKAMEKTMKRAGSIKKYYVP
                     GLFIFVAIPLPGTGVWAGSLAAALLNIRLLHALPAIFLGNAVAGLIITLISYGIIGAI
                     "
     gene            complement(2592..2676)
                     /locus_tag="PLU15_09425"
     tRNA            complement(2592..2676)
                     /locus_tag="PLU15_09425"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2640..2642),aa:Tyr,seq:gta)
     gene            complement(2829..3767)
                     /locus_tag="PLU15_09430"
     CDS             complement(2829..3767)
                     /locus_tag="PLU15_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOO31178.1"
                     /translation="MYKQCVTRGLSHLVPLEGVRLVEDALAAKVPLDTVYIAEDVDMG
                     NESTSGRILSGARRNGARIVIVSTSVAESMADTRTPQGVFALAKWSPASGCELLASAG
                     GNGRPSFGVYLDGVSDPGNVGTIIRTAAALGALGVVAGPRCAGFTNPKVIRASMGSIF
                     RMPVAEVKDVDGFFGRAAELGYDIIVSDSEVGGAVDNLVWPGCARPQERSQQVAKPQI
                     VGDSQDTVCLTDRGADGANVAFLVVGSEAFGVSDAVRKHATARVKIEMARDVESLNVA
                     AAAAILIYALRQKVVAWEDARGVRVNDCGLGDQEKV"
     gene            complement(3824..4186)
                     /gene="rplT"
                     /locus_tag="PLU15_09435"
     CDS             complement(3824..4186)
                     /gene="rplT"
                     /locus_tag="PLU15_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOO31179.1"
                     /translation="MARVKRGTNARRRHNQTLKLAKGYTGVNSVSYKRANQAVMHAMD
                     YSYAHRRTRKRDFRQLWIARINAAARMNGMSYSRFISGLKKAGVDINRKMLADLAVND
                     GAAFEQLVAVAREQEAAN"
     gene            complement(4236..4433)
                     /gene="rpmI"
                     /locus_tag="PLU15_09440"
     CDS             complement(4236..4433)
                     /gene="rpmI"
                     /locus_tag="PLU15_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOO31180.1"
                     /translation="MPKMKTNRASAKRYYVTGSGKIMRNKAGRRHKLAAKTPKRKRNM
                     RKDVVLSKPDQKVARELIPYK"
     gene            complement(4475..4996)
                     /gene="infC"
                     /locus_tag="PLU15_09445"
     CDS             complement(4475..4996)
                     /gene="infC"
                     /locus_tag="PLU15_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOO31181.1"
                     /translation="MSRELFVNEAIRAREVRVVDENGEQLGIMPVREAIKLAADRDLD
                     LVEVAPTARPPVCRIMDYGRYKYEQSKREREARKKQHVVDIKEVRMTPKIEEHDFSVK
                     TKSAERFLRDGDKVKVTIRFRGREIVHSDLARQLLMEMAESLSSVGSVERLPKVEGRN
                     MVMILSPKQEQEG"
     gene            complement(5242..7167)
                     /gene="thrS"
                     /locus_tag="PLU15_09450"
     CDS             complement(5242..7167)
                     /gene="thrS"
                     /locus_tag="PLU15_09450"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO31182.1"
                     /translation="MENVKVTLPDGAVIELPSGTTVGELASRIGRRLAAKAVVGLVDG
                     VAVDLSFPISEDCHVTILDATSPEGLNTMRHSAAHVMAQAVKRLFPGVKLAIGPTIDD
                     GFYYDFDIDHAFTSEDLEKIEAEMAKIIAEDLPIVRQEVSWVEAMDVLKSGDEVYKME
                     LLNELGDGEVSFYRQGEFCDMCRGPHLPRTGMIGAFKLLSVAGAYWRGDENRPMLSRI
                     YGTAFPTRKELDEHLRLLEEAAKRDHRKLGKELDIFSVHEEAGAGLIFYHPHGAAIRH
                     SIEDFWMREHIARGYKPVITPHIAESTLWTISGHNDYYRENMYYLSIDEKEYVLKPMN
                     CPGHILIYKTATRSYRDMPIRYCELGTVYRYERSGALHGLLRVRGFTQDDAHIFCTPE
                     QLRDEIVGVIDLTQFMLKSFGFSEYEFFLSTRPEKSVGSDENWERATAALRDALEGHG
                     LKYTVDPGEGVFYGPKIDTKLKDSLGRLWQGPTIQVDFNLPERFDVNYIGEDGEKHRA
                     IMIHRTVLGSMERFMGCLIEQYAGALPVWLSPVQAKVLSIGDRHIDYAMGIARNLSER
                     GFRVDTDVRDEKIGYKIREAQLEKTPYMLIVGDREMESGQVAVRARRGGDLGAMTLNQ
                     FADRLRAEVDSRTCEDV"
     misc_binding    complement(7287..7510)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7640..8569)
                     /locus_tag="PLU15_09455"
     CDS             complement(7640..8569)
                     /locus_tag="PLU15_09455"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HOO31183.1"
                     /translation="MDCVHIGSYLAADSLRRRLFNELDGMRAQGLDVSVEIKYLGGLT
                     MIECMASARPERPLDDSELRSIFRRRLANVVARLIFEELEKSFILETLRHRHPEIDDN
                     EAGEVSGYAIAILNDGIFGMLEPAIRLSEVAADIEAYLRDSSLLVVEGFVRFRMREYL
                     DELRSSTEEAIGKFVAERERKEFVKLLKYFVDVQEERQDEVHVVRDGAGFELKSADGT
                     PIEGDYVASLAADLLGDGIEVVDLLISALITVAPRKVVLHFEPELETADTLDSVFEGR
                     VIKCCNPDCGFSGCEMRIPNRAGKGPVNPIGRR"
     gene            complement(9121..9197)
                     /locus_tag="PLU15_09460"
     tRNA            complement(9121..9197)
                     /locus_tag="PLU15_09460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9161..9163),aa:Val,seq:gac)
     gene            complement(9213..9287)
                     /locus_tag="PLU15_09465"
     tRNA            complement(9213..9287)
                     /locus_tag="PLU15_09465"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9253..9255),aa:Cys,seq:gca)
     gene            complement(9326..9400)
                     /locus_tag="PLU15_09470"
     tRNA            complement(9326..9400)
                     /locus_tag="PLU15_09470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9366..9368),aa:Gly,seq:gcc)
     gene            complement(9414..9489)
                     /locus_tag="PLU15_09475"
     tRNA            complement(9414..9489)
                     /locus_tag="PLU15_09475"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9454..9456),aa:Phe,seq:gaa)
     gene            complement(9510..9586)
                     /locus_tag="PLU15_09480"
     tRNA            complement(9510..9586)
                     /locus_tag="PLU15_09480"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9550..9552),aa:Asp,seq:gtc)
     gene            complement(9613..9688)
                     /locus_tag="PLU15_09485"
     tRNA            complement(9613..9688)
                     /locus_tag="PLU15_09485"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9653..9655),aa:Val,seq:tac)
BASE COUNT         2322 a         2967 c         2268 g         2305 t
ORIGIN      
        1 catcctgtca cctccctcca ttttggctgt gggcgtagaa aattcgctaa atgtctacca
       61 gttccgcctc attaacccgg gtaatcgaaa ccaccgggta aacctgcagc ccggaaagcg
      121 ccaccgcgac gtcataggca tcctggtcgg cggctgtcgg ttttaccccg gaataggtcc
      181 tggtggagta cctggggttt ccctcatcgt cctgccccac ctcgtacctg atccttaacc
      241 tggaatccat cggctgtacc tgcagcggca tattttcacc ccctttcagc tcaattttac
      301 aggggggacc tgccgtgggt aaaaaaacga aagaagcctg tgaggccggt tccacggcag
      361 ggtttggctt cgttttggca aaaaaagtac cgggttatgc cctataaggg cctttacccg
      421 gtactttaag gtacttattc cttgaatcta cctaccgaag gcctggaccc tcaggtcctc
      481 cgccttgtcc gtcctttccc aggaaaggtc aaggtctttc ctgccaaaat gcccgtatgc
      541 agccacgttc ctgtatatgg gccttctgag gtccaaatcc cttattatgg ccgcgggacg
      601 gaggtcgaaa ttgtcatata taagccttat tattttctcc tcgggtattt tggcggtgcc
      661 gaaggtgtcc accataactg agacggggtt tgccacgcct atggcatagg ccagctgcac
      721 ttcgcacttt gaggcgatac ccgaggccac tatgtttttg gccacgtacc tggccgcata
      781 ggaagcggaa cggtcaacct tggtggggtc cttcccggaa aaggcccccc ctccgtgcct
      841 tgcatagccg ccgtaagtat ccactataat ctttcttccg gtcaagccgg aatccccctg
      901 gggacccccc accacgaacc gcccggtggg atttatataa tacttggtat ttttatctat
      961 taactcggcg ggcaccactt ttttgacgac atgctcaata aggtcctccc tgatgattcg
     1021 gctgtccacc ttcggatgat gctgggaaga caacactatg gtatccactc tcaccggctt
     1081 gtccccttca tattcgacgg ttacctggga cttgccgtcc ggtcttaagt aaggcagtat
     1141 atacgacttc cggacttcgg caagcctttt ggtcagcccg tgggccaagt gtatcggaag
     1201 cggcatcagc gtgtcggtct cgtcgcatgc aaagccgaac atcatgccct ggtcgccggc
     1261 acctatggca tccacttggt tggatatgcc ctgctttgct tcataagcct tgtccacccc
     1321 cagcgctatg tccggcgatt gttcgtctat ggatgtcaaa acggcacagg tatcgctgtc
     1381 aaagccaaat tttgccctgg tatacccgat atccctgagg gtctgccggg ctatgctggg
     1441 gatgtccaca tagcattcgg tgcttatctc acccatcacc aataccaagc cggtggtaac
     1501 cgcagtctcg caggcaaccc tggcactggg gtccttctca aggattgcat ccagcaccgc
     1561 atccgaaatc tggtcgcata tcttatccgg atgcccctcg gtaaccgatt cggacgtaaa
     1621 cagttttctt gtcatttacc tatcctcctt ttaatatgcc cctagaagta ttattgcctt
     1681 gtcaaataaa aataaaaaat cctcctctgt aatcaaggag gtcgccgcca ccttatctta
     1741 cagaacctga aattctgtgg gaattggcac cgctgcgcat gctgcgccgg ttgccgggtt
     1801 tcatcgggcc cttatccctc cgcctctctt gataaggcaa atatttggtt ttccgataga
     1861 aaatactatc acagcattta taatatgtca atcatattgc gccaataatc ccgtaactta
     1921 taagggtgat tataagcccc gccacggcat tgccaaggaa tatggccgga agcgcatgca
     1981 aaagccgtat gttcaaaagg gccgctgcaa gactccccgc ccacacgccg gtgccgggca
     2041 aaggtatcgc gacaaaaatg aaaagccccg gcacatagta tttcttaatg ctgccagccc
     2101 tcttcattgt cttttccatt gctttttcgg taaggcttcg gaacaggccg gttcttttta
     2161 caaaattaaa aaagggtcga aacaccagca ttataaccgg cgagggaagt atgctgcccg
     2221 caagggcaag aacaaaggca tgcatcgggt gcatacccat cgatatgccc attggtatag
     2281 ccccccttat ttccacatag ggaagcatgg ccacgaaaac caccgccagc tcgtctccta
     2341 tgaactgcag cacatcggtt acaacgccgg tcatggttca cacccccata taatacaaca
     2401 caaaaattat accctataaa aaggtgtatg taaaaccctg caaaaaacgg cgcgtatttt
     2461 cgcgccgcct aaaaccgaaa agggacaagg gacggttctt tgtcccataa aaagcagcgt
     2521 agtattcatg aagtctaacc gtataaggtt acaaatagga acggcgctta ctttagcgcc
     2581 gcttaaaacc ttggtggagg gagatggatt cgaaccatcg aagtcttcga caacagattt
     2641 acagtctgcc ccctttggcc acttgggtat ccctccatga tggagctggt gatgggactc
     2701 gaacccgcaa cctgctgatt acaaatcagc tgctctgcca attgagctac gtcggcaagc
     2761 aatcgtgccg tgcgaagctt agaataccac atgcgtcccc ataagtcaac aacctggaca
     2821 cgctactttt aaaccttctc ctgatcccct agaccacagt cattgacgcg cacaccacgt
     2881 gcatcttccc aggcaacaac cttttgcctc aatgcataaa tgagaatagc tgcggccgca
     2941 gctacgttca gagactccac gtcccttgcc atctcgattt tcacccgtgc agtggcatgt
     3001 ttcctcacag catcgctcac tccgaaggcc tcagaaccta ccaccagaaa ggcaacgttt
     3061 gcaccgtcgg ccccccgatc tgtcaggcaa acggtgtctt gcgaatcccc gactatctgc
     3121 ggtttggcga cttgctgcga cctctcctga ggccttgcgc aaccaggcca gacaagatta
     3181 tcgaccgccc ccccgacctc cgagtcggac acgatgatgt cgtacccaag ctccgcagcg
     3241 cggccaaaga agccatcaac atctttgacc tccgcgaccg gcatgcgaaa gatagaaccc
     3301 atggaagccc ttatgacctt cgggttggta aagcctgcac atcgtggacc ggcaaccact
     3361 cccagcgctc ccagggctgc cgcagtgcgt atgatggttc ccacattccc cggatccgag
     3421 actccatcca aatagacccc gaaagacggc cttccatttc caccagcgct ggcgagcaat
     3481 tcacatcccg aggccggcga ccacttcgct agcgcgaaca ccccctgagg cgtgcgcgta
     3541 tcggccatac tttccgcaac tgacgtggat acaatgacaa ttcgtgcacc atttcggcga
     3601 gcgcccgaca gtattcgacc actggtcgat tcattgccca tatcgacatc ttcggctatg
     3661 tagactgtgt ctagcggcac tttggccgcc agcgcatcct ccaccagcct tacaccctca
     3721 agtgggacaa gatgcgataa accccgagtc acgcattgtt tatacatgcg gacagcgggg
     3781 ttcttttggc tcctgattac gtccaatcta ctcaccgctc acactagttt gcggcttcct
     3841 gttccctagc cacagccacg agctgttcaa aagcagcgcc atcattaacg gccaggtccg
     3901 ccaacatctt gcgattaatg tcaactccgg cctttttcag tccagaaatg aacctgctat
     3961 atgacatgcc attcatgcgg gccgcagcat tgatccgcgc aatccaaagc tgcctgaaat
     4021 cgcgcttacg cgtgcgcctg tgggcatatg aatagtccat ggcatgcatt actgcctggt
     4081 tagcccgttt atatgaaacg ctgttaactc cagtgtaacc ctttgcgagt ttcaaagttt
     4141 ggttgtgcct gcgccgcgcg ttcgtgccac gctttaccct cgccatcgta aatcaacccc
     4201 ctatggccat catgcaaaac aacctatcag aaaagctact tatacgggat cagttcgcgc
     4261 gcgaccttct gatccggttt cgaaagaaca acgtccttcc gcatatttcg cttcctcttg
     4321 ggggttttgg cagcaagctt gtgcctacgc ccggccttgt tccgcatgat cttaccggat
     4381 ccggtcacat aatatctctt cgccgatgct ctgttagttt tcatcttagg cataaccatt
     4441 gccctcctca actgaatcca cgcaaaaaac agacctaccc ctcttgctct tgcttagggg
     4501 aaagaatcat aaccatgttt ctgccttcaa cctttggcaa gcgctctaca gaacctacac
     4561 ttgaaaggct ctccgccatc tccatcaaaa gctgcctggc gaggtcgcta tggacaattt
     4621 ctcgtcctct gaaccgtata gtaaccttaa ccttgtcgcc gtcacgaagg aaccgttccg
     4681 cactcttagt cttgacactg aagtcgtgct cttcgatctt cggagtcatc cggacttcct
     4741 ttatgtcgac aacatgctgc ttcttccttg cctcccgctc gcgtttgctc tgttcatact
     4801 tgtatctgcc ataatccatg atccggcaga cgggcggtcg tgctgtaggt gcaacctcca
     4861 cgaggtccag gtccctatcc gcggcaagtt tgatcgcttc cctgacaggc attatgccca
     4921 gttgttcacc gttttcatcc acgacgcgga cttccctggc cctgatcgcc tcgttgacga
     4981 aaagctccct actgatagtt tgtcaccccc ataaaaattt cagtgtttga atgcccccaa
     5041 aataaagaac aagcgggtga tccacccgcc cacaatgaca gagcatacgg cgctcggcaa
     5101 atttgccggt ccgcatatag tcaagtgaac cgacctggct gaagcgttga gcaacgccga
     5161 tcggtgagaa gcggatggct tctacttcaa acatgtatcc cagattcgca agaccgagta
     5221 taacacaggc atggccctgc gtcaaacatc ctcgcacgtg cgagagtcaa cttctgcgcg
     5281 aagcctgtcg gcgaactgat tcaaagtcat ggcacccaga tcaccgcctc ggcgcgccct
     5341 cactgcaacc tggccggatt ccatttccct gtcgcctacg atgagcatgt acggagtctt
     5401 ttcaagttgg gcttcgcgta tcttgtatcc gattttctca tctcggacat ccgtgtcaac
     5461 cctaaaccct cgttccgaca agttcctggc aattcccatg gcatagtcga tatgtcgatc
     5521 gccgatggag agaacttttg cctgaactgg agagagccat acaggcaagg cgcctgcata
     5581 ctgctcaatc aaacacccca taaaccgctc catgctgcca aggacggttc tgtgtatcat
     5641 gatagccctg tgtttctctc catcctctcc gatgtagttt acatcgaacc tttcgggcaa
     5701 gttgaaatcc acctgaattg ttggcccctg ccagagacgg ccgagagaat ccttgagctt
     5761 ggtatcgatc tttgggccgt aaaacacgcc ctcacctgga tctacggtat acttaagccc
     5821 gtggccttct aatgcatccc ggagagccgc tgtcgcacgc tcccagtttt cgtccgatcc
     5881 caccgacttc tctggccttg ttgacaggaa aaactcgtat tccgaaaatc caaagctctt
     5941 cagcatgaac tgggtaagat caatcacccc tacaatttca tcccgcagct gctcaggggt
     6001 gcaaaagatg tgagcgtcat cctgagtgaa tcccctcacc ctgagcaacc cgtgaagagc
     6061 cccagaacgt tcatatctgt acacagtccc cagttcgcag tagcgtatcg gcatatcccg
     6121 atagcttctc gtagcagtct tgtatatcag tatatgcccg ggacaattca tgggcttcag
     6181 aacatactcc ttctcgtcta tggacaggta atacatgttc tcccggtaat agtcattgtg
     6241 ccctgatatg gtccatagcg tcgattctgc aatgtgcgga gtgataacag gcttataccc
     6301 ccgcgctatg tgttcacgca tccaaaagtc ctcaatagag tggcggatgg ctgcgccatg
     6361 agggtgatag aagataagcc cagcgcctgc ctcctcgtga acgctgaata tgtcgagctc
     6421 cttgccgagc tttctatgat ccctcttggc agcctcctcc agaaggcgca gatgctcgtc
     6481 gagctctttc cgggtgggga aagcggttcc gtatattctt gagagcatgg gcctgttctc
     6541 atcgcctcgc cagtacgccc cggccacgct gagaagcttg aaggcgccga tcattcccgt
     6601 tctcggcaga tgaggcccac gacacatgtc gcaaaactcg ccctgtcgat agaagctgac
     6661 ctcaccatca ccgagttcat tgaggagctc catcttgtag acctcgtcgc cactcttcaa
     6721 cacgtccatg gcctcgaccc atgacacctc ttgacggact attgggagat cctcggcgat
     6781 gatcttcgcc atttccgctt cgattttctc aagatcctcg gaagtaaagg catggtcgat
     6841 gtcgaaatcg taatagaacc catcatcgat cgtcggccct attgcgagct ttactccggg
     6901 aaacagtctc ttcacagcct gtgccattac atgcgccgcg gaatggcgca ttgtgttaag
     6961 cccctcgggc gatgttgcgt ccagtatagt cacatggcaa tcttcggaaa tagggaatga
     7021 gaggtcaacg gctacaccgt ccacaagacc gacgaccgcc ttcgcggcca atcgcctgcc
     7081 gatgcgggaa gccaattcgc ccactgtagt tccggaagga agctcaataa cggcaccgtc
     7141 cggcaaagtc actttcacgt tctccatcag tctcctgccc ccttgcacgt ttcaatgctc
     7201 agccaactca atgtacaatc cagccatcag ctcgtaacta cgcatgccaa ctggaaaaac
     7261 aacaaccccg tccctaaatg atccatcatc gggacggggt taacccgcgg ttccacccgg
     7321 cttggcgcag atcgcgccct cttaagagcc acataacggc ggccagccgt gccagcctaa
     7381 tcccgcctca gcggtttcag cagcaagctc agaggtggtt ttcagcaatc tacaccccgg
     7441 cagactccca ccgcatggcc cgccgtctct gagggttcca aagcttactc gtcctcatca
     7501 tcgccatata cgcattcaat tagatctatt ctagccctgc aaagccacag atgtcaatac
     7561 aggctcaacg cccgacccac agcccgcccg caaagtggca gcagagccaa ccccgaagcg
     7621 ggtacggggt cagatcatat caccgccttc caattgggtt tacggggcct ttgccagccc
     7681 tattcgggat gcgcatttca cagcctgaaa acccacaatc tgggttgcaa cactttatta
     7741 ctctgccttc gaatacgcta tccagcgtat ctgcggtttc caactctggc tcaaaatgga
     7801 ggactacctt tcgaggagcc acagttatga gggcgctaat caagagatct acaacctcaa
     7861 ttccgtcgcc caacaaatcc gctgctaaag aagcaacata atctccttct atcggcgttc
     7921 cgtctgctga cttgagctcg aatcctgcgc cgtctcgaac cacgtgaacc tcgtcctggc
     7981 gttcctcctg aacatctacg aagtacttca aaagcttaac gaactctttg cgttcccgtt
     8041 cagccacgaa cttgccaata gcctcttccg tagagctccg caattcgtcc aaatactccc
     8101 tcattctgaa gcgcacaaag ccttcaacca ccaacaggct agaatcgcgc aaatacgcct
     8161 ctatgtcggc tgccacttca ctcagacgaa tcgcaggttc cagcattcca aagatcccgt
     8221 cattaagtat ggctatggca tagcccgaca cttccccagc ctcattgtca tcaatttcgg
     8281 ggtggcgatg tctgagagtc tcaagaatga aagatttctc caactcctcg aaaatcagac
     8341 gagctacaac attggcgagc cgccgccgaa atatggagcg cagctcacta tcgtccaacg
     8401 gcctctccgg gcgagcgctg gccatgcact ctatcatcgt caacccgccc aggtatttga
     8461 tctctaccga cacatccaag ccctgagcgc gcataccatc caactcatta aagagccgcc
     8521 tcctcaacga gtcagcagca aggtaggagc cgatatggac gcagtccatc ccgctttgcc
     8581 ccccttcagc gactagtata tgtcggggat aacaagcgta tacgatgggc agacacgggg
     8641 cataatgtct caatacatgg catacgggtc aatatcgtga ttaaatgtct tttcccggat
     8701 tctgaggcgt gtcgaaaaaa cgccctacgg gcaaatcgtg tacacagcaa cagagctaca
     8761 ccacctgtca catgcagagg cgcgtccctt tttgaggttt ggaggaagac gcctgatcct
     8821 aaatgtcaaa aacctcgcta cagtcctttt aggcaacctg caaccgaagg cgatatccga
     8881 aacagcacgc atatcttgcc cgaaaagcct tcctccatgc gcaacgacat ctgtgcctga
     8941 agcgcgtgcc cttagagcca gatactgctg aaacatgtcc gcagagtctg catgtggctt
     9001 gcataccaat tgcagggtaa gcggcgtcca agacatcgac cgggagcgat cgtgctggac
     9061 atgaaaaggc cccgaggctt acctgcatca aggcaattcc tcggggctga aaccattcaa
     9121 tggtggggtg cagtggagtc gaaccactgg cctcaacgat gtcaacgttg cgctctaacc
     9181 aactgagcta gcaccccaaa accgatctaa tttggaggcg acacccggat tcgaaccggg
     9241 gaatagcggt tttgcagacc gctgccttac cacttggcta tgtcgccggc aaatgacacg
     9301 gccatagggc cgtgtggaaa atctatggag cggaagacgg gattcgaacc cgcgaccctc
     9361 gccttggcaa ggcgatgctc taccgctgag ccacttccgc atataacccg acatggtgcc
     9421 gaggggcaga atcgaactgc cgacacgggg attttcagtc ccctgctcta ccgactgagc
     9481 tacctcggct cagcattgac cgaaaataat ggcggaggcg acgggactcg aacccgcgat
     9541 ctcctgcgtg acaggcaggc atgttcaacc gctacactac gcctccgcac aacttaaaac
     9601 cttatcttaa agtggtgggc gaaacagggc tcgaacctgt gacctcctgc gtgtaaggca
     9661 ggcgctctcc caactgagct attcgccctt tgccgcccgg cgcttaatta ttataacgta
     9721 cgacctcgag gcatgcaagg gggcaaaatc actttgttac gcgtctgtga tattgtcacc
     9781 ccataacgcg tctgagataa tgtcacccat gaacaagggg gacatcatct tgacggaaaa
     9841 attacggaag agggtcttcg ta
//
LOCUS       DAOSYL010000073         9794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5894_length_9794_cov_7.029777, whole genome shotgun sequence.
ACCESSION   DAOSYL010000073 DAOSYL010000000
VERSION     DAOSYL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5894_length_9794_cov_7.029777"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..873)
                     /locus_tag="PLU15_09490"
     CDS             complement(466..873)
                     /locus_tag="PLU15_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31184.1"
                     /translation="MQLVLDQSVLCQDVVGVNAAYRGYAITGRLIWSVACYRYDGHDP
                     GALWKTKSSSNPVPHLRPGMDRDLVGNRVEFMEGRHYLLHVRLIRFNSSIEELGHLLK
                     AVVSIIHGPCPPFIIRSSNEIRQGSEESSCSAK"
     gene            1417..2607
                     /locus_tag="PLU15_09495"
     CDS             1417..2607
                     /locus_tag="PLU15_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO31185.1"
                     /translation="MIKSSRGKMKIICLAVAAALLLTAATGLGRSAKLTPIKIGAAGP
                     YTGDVSKIGLDGLNAIRMAVDEANAAGGIGGRKIEIVEADDAADPSKAILVAEKLAMD
                     RSVIGVIGPMNSATAQAALPTYQRAGLAVISQSATNPDLTEMGYTVMHRICPRDDAQG
                     PASARFIIEVLKAKTVYIIDDKTTYGQGLSDQVEPALKAAGVRVLRGQISMEDRDFSP
                     ILTRVKSSAPDLVYLGLANPAQAASLIKQAAGLGLKPVFMGGDGLKEKDQLIDGAAGL
                     AEGMYVTSIGRDIREVPEARNFIRKFESKYGALSVFGGQSYEAANILIDAARRAHSDP
                     MKITRANVLAALGKTRGYKGILGFPISFDSKGDVVGASIFVYQVKGKEFVPVKEYPAT
                     VRAK"
     gene            2696..3622
                     /locus_tag="PLU15_09500"
     CDS             2696..3622
                     /locus_tag="PLU15_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO31186.1"
                     /translation="MEQFLAQVPQQIVNGITLGSVYALLALGYSMVYGVLKMLNFAHG
                     DVFMVGAFAGWAVLSLFEQNGLIAMPALALAAMALGAIVATALLGAAIERFAYRPLRG
                     GSRLAPLISALGVSIILENAAMLLTSGRAKTFPTELLIPQDMVVHVAGAAISATRLAM
                     LGTSILAMVILDAVVLKTKFGRAMRATSQDAEAAEYMGIRTSLVISATFMLGSALSGL
                     GGVMIGLYYTQVDFMIGFSAGMKAFTAAVLGGIGSFRGAVVGGLLLGLAESFGVLIAP
                     PVYKDAIVFAVLILTLILRPHGLYGKPQQARA"
     gene            3692..6571
                     /locus_tag="PLU15_09505"
     CDS             3692..6571
                     /locus_tag="PLU15_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HOO31187.1"
                     /translation="MSNECVNTTEKHPHSASLRQTEAEQAQVAPEAATRASNSAKAAD
                     GPNLAGGLLRIGLLERLGLWQSGRVPIERALVFLGYAVLLPVPLITKNYYLMRAGGSV
                     GIYLMLAAGLSIVAGQAGLLDLGYAGFYGIGAYVYALLASPQFGIHMPFFVAAAASVA
                     AAVIAAFAVSLPTLRLHGDYLAMVTLGFGQIVRILLNNMDRPINITGGPNGIVAIDPP
                     HIFGSSLASMESQYILIWIFAAAVLIGVSRLAGSKVGRAWNALRENETAAECMGVNVR
                     KYRVSAFAGAAAIAGLAGALFASWQGAVFPQNFTMSETIALYCMLVLGGTRSVPGIML
                     GVLLLQVLPEVLRAYSVYRMLIYGFALVLLAIFRPQGLIATGERLVGVDPTRAHGAHP
                     GPGICLGTNANAGLAERTTDDAVSASSCPIPDNGGEPEAIRSASTSASPGQATVLEVT
                     GLSCRFGGVQALSDVSFTVKRGEILGIIGPNGAGKTTLFNLITGLANPSAGDIRLYGQ
                     SIVGKSPHEVAAMGAARTFQNIRLYETQTVLENVLAGRHLKQDAHLWEVLFRTKSHVR
                     KEEEDILCVRHILSDMDCGLEKRESDIVAELSYPDKRRVEMARALATGAKFILLDEPA
                     AGMTSEEIAAMAREIVALRDRGYTIVVIEHHMDLIASACDRVIVLDHGEKIAEGSCSE
                     VAKDQEVIRAYFGSSAAPDRKPAETLASNAMSTSSAATSGLPTPLLELIDVNSSYGPA
                     VVLQGANLYVNPGEAVALLGVNAAGKSTLLKTIAGRLRPTRGEIRFMGERIDGRETNE
                     IIRMGMGVVPEGRRVFQELTVMENLEVAADALGDPEGAREGIDRALSLFPVLADRRSQ
                     LAGTLSGGEQQMLAIARALVTSPRLICMDEPSMGLAPIMVERVMGAIAEINRAGTAVL
                     LVEQNAKAALSVADRAYILKDGRTSAPSNPNQILSGEAGKVASIF"
     gene            complement(6670..7455)
                     /locus_tag="PLU15_09510"
     CDS             complement(6670..7455)
                     /locus_tag="PLU15_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOO31188.1"
                     /translation="MEEYEQNALSSTRHVIGVMSGKGGVGKSSVTALTAVALARMGYK
                     VGVMDADITGPSIPKMFGVHGKLDSAETGLFPAQTERLGIRIVSLNLLLDREDRPVVW
                     RGPLLAGAVKQFWTDVSWGDLDFMIIDLPPGTGDIPLSVMQSIPVEGLITVSSPQDVA
                     ALVVRKAVNMAEMMDKPIIGLIENMSYIKCPGCGETMYPFGKPAGAGFASEMGIPFIG
                     EMPIDPALSEAADRGEIEMYESPIMQGIAEAVVRHVQASAQEE"
     gene            complement(7562..8146)
                     /locus_tag="PLU15_09515"
     CDS             complement(7562..8146)
                     /locus_tag="PLU15_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOO31189.1"
                     /translation="MGRPPKVRCVEMIPPVRTFCPCGNWPECRAHRGADERIDMTVDE
                     MEAIRLKDLEGCEQTECADRMNISRGTFQRILSSARWKVAKALTEGLILQISGGEYCL
                     AQSTIICLDCGHAWPVCRPNADSKDECEETVTAEDGTVSGSCPECRGTNLVEKGGECS
                     GPGGREDCPRRRERGRRGCSHRSGAEESAAKSTD"
     gene            complement(8752..9120)
                     /locus_tag="PLU15_09520"
     CDS             complement(8752..9120)
                     /locus_tag="PLU15_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOO31190.1"
                     /translation="MDKPVTTAHARSELMQLLLVFRDEREWGQFHTPKNLAIALVAEA
                     SELLSLFQWTKDAELDERCREKRTEIEQEIADIYIYLLFLANELGIDLDEVAAEKMKL
                     NAEHYPANKAKGNARKYTEL"
BASE COUNT         2093 a         2824 c         2751 g         2126 t
ORIGIN      
        1 ttccgttcta acgcgctctg tcttcttcca acggcagccg cccgcgccca gcgggtattc
       61 aggccatacg ctcccgccta gcagggcaca agagccgcaa tcttcgactg gctcggcagt
      121 atagctagcc gaactgaact ggcagcgcca ggcaacaagt tgattgattc ttttcagggc
      181 ctcgcaagtc cttcttcttg gcatttacag tgcagaacgc cggcgcatcg tcctgaagct
      241 ggaatccatg gcagagcgac ctgctggcgg ctcgaaacgc tccgtcgact atggtgcgct
      301 ataggcagta gccagagcca atggcagaat cgacggggga aaacttactt cgaaaggaac
      361 atgtcaggcc cctgcgggcc agggcgtgtc tatgccagag ttagaggcac cgacaacgcc
      421 atacgggctg agcgcgttcg tcgcgtgact ttttatcctc agtctttact ttgccgaaca
      481 cgaactctcc tcgctgccct gtctgatttc gtttgagctc cgtattatga agggaggaca
      541 tgggccatga atgatggaca caacggcttt gagcaaatga ccaagttctt cgatagaaga
      601 gttgaaacgt atgaggcgca catgcaggag ataatgccga ccttccatga attctactct
      661 gttgccaacc agatccctgt ccatccccgg cctcaggtgc ggcacgggat tggagctcga
      721 tttcgttttc cagagggcgc caggatcatg gccgtcgtat ctgtaacacg ccacgctcca
      781 aatcaacctg cccgttatgg cgtaccctcg ataggccgcg ttcacgccta ctacatcctg
      841 gcataacacc gattggtcta gaaccagttg catccgtatg cttccatcgg gactccactc
      901 ggggcaagaa actgcctctt gacaactcca ctcctctggt gttaaatagt acgaaactga
      961 atcatgtgtg gatgaaaaga ctttgatggg gaagatcgcc gcagaaaaac catcccatag
     1021 cgagcggggg agagtgaaag ccctgcggga aggatgtggc gagtgggccc cggagtcgca
     1081 ggccgaaagg cgcacaatgc gtgcgtggag cctagtaggc tgagccggaa gcccccgtta
     1141 tcgggcaggg gcatcgcctt caccgagatg gaggccgcgc cctggataag tgggataggt
     1201 tatcctatcc aattagggtg gcaccacgag atttgagctc gtcccttggc ggacgggctt
     1261 ttttgcgtac cgtcgaagcc cgaggcatcg cggctctcaa tgtggttgtc cccgagcgca
     1321 gccctctggc cctggccgcg tggccaggct gaaaggtgcg acagcatcgg ctttcatcca
     1381 actgaccagt caagcaggaa aaaggaggcc actaccatga ttaagagttc tagaggcaaa
     1441 atgaagatca tctgccttgc cgtggcggcg gcactgttgc ttacagccgc aacagggctg
     1501 ggccgatcgg caaagctcac tcccatcaag ataggggctg cagggcctta caccggcgat
     1561 gtgtccaaga tcgggcttga cggattgaac gccataagga tggccgtgga tgaggcgaat
     1621 gcagcaggcg gaatcggggg gcgcaaaatc gagatagtgg aggcagacga tgcagcggac
     1681 ccgtccaagg cgattctggt agcggaaaaa ctcgcgatgg accgttcagt catcggtgtt
     1741 atcgggccca tgaacagcgc caccgcccag gcagccctcc caacgtatca gagagcaggc
     1801 cttgccgtca taagccagtc ggcaacgaat cccgacttga ctgagatggg ctacacggtg
     1861 atgcacagga tctgtccgcg cgacgatgct caggggccgg cttcagcccg cttcataata
     1921 gaagtcctaa aggcaaagac ggtatacatc atagatgaca agaccaccta cggccaaggc
     1981 ctttcagacc aggtggaacc ggccctcaag gctgcaggcg ttagggttct ccgagggcag
     2041 atatccatgg aggacaggga cttctccccc atcctcaccc gggtcaaatc atccgcgccc
     2101 gatttggtct acctcggctt ggccaacccc gcacaggcgg ctagcttgat caaacaggcc
     2161 gcgggcctcg gactcaagcc ggtatttatg ggcggcgacg ggctaaaaga gaaggaccag
     2221 ctgatcgacg gcgcagcagg gcttgccgaa ggaatgtatg tcacctccat aggccgagac
     2281 atcagggagg ttcctgaagc ccgcaacttc atccggaagt tcgagtcgaa atacggggcc
     2341 ctgagcgtct tcggcgggca atcgtacgag gccgccaata ttctgataga tgcggccagg
     2401 cgagcccatt ccgacccgat gaagataacc cgggccaatg tcctcgcagc gctgggcaag
     2461 acccgcggct acaagggcat cctcggattc ccgataagtt tcgactcaaa aggcgatgtg
     2521 gtgggagcca gcatcttcgt ctatcaggtg aagggcaaag agttcgtgcc tgtgaaggaa
     2581 taccctgcga cggtgcgagc caagtaggcc ggcgccacgc tcgtccttaa aggttagccc
     2641 aagggcccaa ttccgtaaaa cgcggcgtcc gcaaagctca attgaggtga atttcatgga
     2701 acagtttctg gctcaagttc cgcaacagat agtaaacggg atcacgctcg ggagtgtata
     2761 cgccctcctc gccctgggct actccatggt ttacggggtt ttgaagatgc tcaacttcgc
     2821 ccacggtgac gtcttcatgg tcggggcctt cgcgggctgg gcagtcctct ccctcttcga
     2881 acagaatggg ctcatagcca tgcccgctct ggctctggct gcgatggcgc tgggggctat
     2941 agtcgcgacc gctcttctcg gcgcggcaat tgaacggttc gcttataggc cgctccgggg
     3001 cggttctcgc ctcgcccccc tcatctcagc ccttggcgtt tccatcattc ttgagaacgc
     3061 agcgatgctc ctcacgtctg ggagggccaa gactttcccg actgagctct tgatcccaca
     3121 ggacatggtc gtccacgtcg caggcgcggc catctctgcg acacgtctcg ccatgttggg
     3181 cacgtccatc ctggccatgg tcatcctcga tgccgtggtg ctgaagacta agttcggccg
     3241 ggcgatgcga gcgacgagcc aagacgcgga ggcggcggaa tacatgggaa tccgaacgtc
     3301 gctggttata tccgccacat tcatgctcgg ctcggcccta tcgggactgg ggggggtaat
     3361 gataggcctg tattacaccc aagtagattt catgatcggg ttttcggcag gaatgaaggc
     3421 cttcaccgcg gcagtattgg ggggaatagg gagcttccgt ggagctgtag taggaggact
     3481 cctgctcggg ctggcggaaa gcttcggggt gctgatcgcg cccccggtct acaaagacgc
     3541 catcgtcttt gctgtgctaa tactcaccct catcctccgc ccccacggac tctacggtaa
     3601 accccagcaa gccagggcgt gaggagcaag cacgatggcc aaccgatgag gcaacggtat
     3661 acaatcacta atctgaaagg cggcatagcc gatgtccaat gaatgcgtga ataccactga
     3721 aaagcacccc cacagcgcat cgctccggca aacagaagcc gagcaggcgc aggtggcacc
     3781 ggaagcggcc actcgggcgt caaattccgc caaggcagct gatgggccca atctcgccgg
     3841 cggactgctt cgaatcggac ttttggagcg gttgggcctg tggcaaagcg gacgcgtccc
     3901 gatcgagcgc gccctcgtct tcttgggcta tgccgttctc ttgcccgtcc ccttgataac
     3961 gaagaactac tacctgatgc gtgcgggagg gtctgtcggc atatacctga tgctggcagc
     4021 ggggctttcc atcgtggcgg gacaggcagg cctcctcgat ctgggctatg cgggattcta
     4081 cgggatcgga gcatatgtat acgcgctgct cgcatccccc cagttcggga tacacatgcc
     4141 ctttttcgtg gcagccgcgg catctgtggc ggcggcggtg atcgcggcat ttgccgtgag
     4201 cctgccgacc cttcgtcttc acggtgacta cctggccatg gttaccttgg ggttcggcca
     4261 gatagtccgt atactcctga acaacatgga tcgccctatc aacatcacag gcggcccaaa
     4321 cgggatagtc gcaatagatc cgccgcacat cttcggctcg tctttagcgt caatggaatc
     4381 gcaatacatt ctcatctgga ttttcgccgc ggcggtgctg ataggcgtat cgaggctcgc
     4441 aggttccaag gtgggccggg catggaatgc gctgcgtgag aacgagactg ccgcggaatg
     4501 catgggcgtg aacgtgagga aataccgagt gtcggccttc gcaggcgctg cagcaatcgc
     4561 cggacttgcc ggagcactct ttgcttcctg gcagggggcg gtctttcctc agaatttcac
     4621 tatgagcgag accatcgccc tctactgcat gctcgtgctg ggcgggactc gaagcgtgcc
     4681 agggataatg ctgggagtgc tgcttcttca ggtgctgccc gaggttcttc gcgcatacag
     4741 cgtctatcgg atgctcatat acggcttcgc cctggtactg ctcgcgatct tccgccccca
     4801 aggcttgatc gccacaggcg aaagactggt cggggtcgac ccgacccgag cacatggcgc
     4861 acaccctggc ccaggtatat gtctgggcac aaatgcgaac gcaggtcttg ccgaaagaac
     4921 aacagatgat gccgtgtctg cttcatcctg ccccataccc gataatggcg gcgagccgga
     4981 agctatccgc agcgcgagca catcggcgtc gccagggcaa gccacagttc tcgaggttac
     5041 ggggttaagc tgccgtttcg ggggagtgca ggctctttcg gatgtgagtt tcacggtcaa
     5101 gcgcggcgaa atcctcggga taatcggtcc aaacggagcg ggtaaaacga cccttttcaa
     5161 cctgattaca ggccttgcga acccaagtgc gggcgacatc agactgtacg ggcaatcgat
     5221 cgtaggcaaa tccccgcacg aagtggcagc gatgggagcg gcgcggacgt ttcagaatat
     5281 aaggctttat gaaacacaaa ccgtcctcga gaacgtcctc gccggacgcc acctgaagca
     5341 agacgcacac ctttgggagg tgcttttcag gacaaagtcc catgttcgca aggaagagga
     5401 ggacatcttg tgcgtaaggc acatcctttc ggacatggat tgcggcctcg agaagcggga
     5461 atctgacatc gttgcagagc tttcataccc tgacaaacgc cgagtggaga tggcccgagc
     5521 gctggccaca ggtgcgaagt tcatcctcct cgacgaacct gccgccggga tgacgtcgga
     5581 ggagatcgcg gcgatggcga gagagatcgt ggcgcttcgg gaccgcggct acaccatcgt
     5641 cgttatcgag catcacatgg acctgatcgc gtcggcgtgc gatagggtga tcgttctgga
     5701 ccacggcgaa aagatagctg aaggttcatg cagcgaagtg gcaaaggatc aggaggtcat
     5761 ccgcgcctac tttggcagtt ccgcggcgcc agatcgaaag ccggctgaaa ccttggcatc
     5821 gaatgccatg tcgacgtcgt cagctgctac gtcggggctt cccacgcccc tcctcgagct
     5881 gatagatgtc aattcatcat acggccccgc agtggtcctg cagggtgcaa acctctacgt
     5941 caatcccggc gaagcagtgg cgcttttagg agtgaacgct gcaggcaaga gcaccctgct
     6001 aaagactatt gcaggaaggc tccgccccac tcggggggag atccggttca tgggcgagcg
     6061 gatagacgga cgggaaacca acgagatcat caggatgggc atgggagttg tgccggaagg
     6121 acgaagggtc tttcaggaac tcacggtgat ggagaacctg gaagtggcgg ctgatgcctt
     6181 aggcgacccg gaaggggccc gggagggcat cgaccgagcg ctctccctct tccctgtcct
     6241 ggcagacagg agatcacagc tcgccggaac cctgtccggg ggcgaacaac agatgctggc
     6301 cattgcaagg gctctcgtca catcgccccg cctcatatgc atggatgaac cctccatggg
     6361 gctagcacct ataatggtgg aaagggttat gggggccatt gccgagataa accgggctgg
     6421 aaccgcggtt cttctggtgg agcagaacgc gaaggccgct ctttcagttg cggaccgagc
     6481 ctacattctc aaggacggca ggacatcggc gccttccaat ccgaaccaga ttctgtcggg
     6541 cgaagctgga aaggttgcaa gcatattttg attgatccaa gaagcagggc tatctttgca
     6601 gaccgccctg cttcactctc tcttttcagg agaaacttgt gtgaatctct ctcgcgcagc
     6661 gccgacgcgc tactcttctt gcgcactcgc ttgtacgtgc cgtacaacag cttcggcgat
     6721 cccctgcatg attggactct catacatctc gatttcgccc ctatcggctg cttcagatag
     6781 agcgggatcg ataggcattt ccccgatgaa cggaattccc atttcagagg caaatccagc
     6841 gcctgccggc ttcccaaagg gatacatcgt ttcgccgcat ccagggcact tgatgtagct
     6901 catgttctcg ataagcccga ttatgggttt gtccatcatc tcggccatgt tcaccgcttt
     6961 gcgaacgacg agagccgcta cgtcctgcgg ggatgaaacc gttatcagcc cctcgaccgg
     7021 tatcgactgc atgactgaaa gcggaatgtc gcccgttccc ggcggaaggt cgattatcat
     7081 gaaatcgaga tcaccccagc taacgtctgt ccaaaactgc tttactgctc cggcaagaag
     7141 aggaccgcgc catactactg gcctgtcttc cctgtccaaa agcaagttga gggagactat
     7201 cctaatcccc agcctctctg tttgcgccgg aaagagccct gtctccgcag aatcaagttt
     7261 gccatgaaca ccgaacatct tgggtatgct agggccggta atgtctgcgt ccattacgcc
     7321 tactttgtat cccattcggg ccaaagccac tgcagtgagc gccgtcactg acgatttgcc
     7381 tactccgcct ttgccgctca ttactccaat gacatgacgc gtgctcgaaa gcgcgttttg
     7441 ctcgtattct tccattcttc cgtccttctt gcgtctttag tttcggtata cctaccctcg
     7501 tggggcagca caaccaatac ttcagccact atcaccgctt ccgtagatcg agcaatgtcg
     7561 atcagtcggt gcttttggct gctgactctt cggcgcctga gcgatgcgag caaccacggc
     7621 gtccacgctc tcttctgcgc ggacaatctt ccctaccgcc aggccccgag cattctccac
     7681 ctttctcgac aaggttggtc cctctgcact caggacatga acccgatact gtgccgtcct
     7741 cggcagttac ggtctcttca cattcatcct tgctatcggc gtttgggcga caaacaggcc
     7801 aagcatgccc acagtcgaga cagatgatgg tcgattgggc caggcagtat tcaccgccgg
     7861 atatctgcaa gatcaagcct tctgtgagag ccttcgccac tttccagcgg gcagacgaga
     7921 gtatcctctg gaatgtgcct ctcgagatat tcattctgtc tgcacactct gtctgttcgc
     7981 agccttccag atccttgagg cgaatagctt ccatctcatc cactgtcata tcgattcgtt
     8041 catctgcccc acgatgagca cggcattccg gccagttgcc acacgggcaa aaggttctta
     8101 caggagggat catctccaca catctcacct tgggaggtct gcccatgttc tcgcccctct
     8161 taagatgcat ttgtgaaatc gcgcacattc cacaaggata ctacatgcat tttgtgcata
     8221 tgtcaataac atcgagcaag tatatgcacg ctggttctag ttttgctggc agaacgcgac
     8281 gtgattccat tttggtaggc ggcagcgagc cctgatccgg gttggatgag gtcttgttaa
     8341 ttgaagctcg tgccttgcac aacgggcctc aagtccctct tcgcggataa tactgaggct
     8401 ttcgtacagg gcgagcagtg aggtggcagt ctccttcgcc cagtggctgt gagcatggca
     8461 atgtccagat accagcggtc ttccgcgacc tgaggatccc accgggcaat cgcctatggt
     8521 aataggcgca aactctgacg ggtacaacag acacttctgt gttccggtgt agcggcgtcg
     8581 gctccacatc ctacattatg tcagccgtgc gctaaccgga cagccccttt acgcaggcga
     8641 agacctgatc ttcttgttcg atgaagccta ccagggatct ttccatgccc gtggaccagc
     8701 gttaacaccc cctggcgact gaaggcagaa gctgcatcga gccgtgcatt gctaaagctc
     8761 cgtgtatttc ctggcgttgc cctttgcctt gttagcggga taatgctcgg cgttgagctt
     8821 catcttctct gcagccactt catcaaggtc aattccaagt tcgttggcga ggaatagaag
     8881 gtatatgtag atatcggcga tctcctgttc tatttcggtc cgcttttccc gacatctctc
     8941 atcgagctcc gcatctttgg tccattgaaa gagcgaaagc agctccgacg cctccgctac
     9001 aagcgctatc gctaggttct tgggggtatg gaactggccc cactctcttt catcgcggaa
     9061 cacgagcagc aactgcatta gttcgcttcg ggcatgtgct gttgtcaccg gtttgtccat
     9121 cagccgaaaa gagcctcctc aaaatggttt cgggttgcct ggtcgaggaa gtatacgtag
     9181 gtgcctttca tgccgcggga tagaggtgtc cctccaagac tcatcggctg ttcttcccgt
     9241 ggcatggatc tacttgcccg ctgcttcctt atagatggtc tgaaagtacc tcccattgag
     9301 ctatcttctg gactgtgggt tatcgttgta ctgctggtga agttgcaggc agtcccgaga
     9361 gcatgcatgc cccaatgcgc gttcatgctc aatctgggca ttctgatagg ccctacaagc
     9421 tgcgacctct gcagacattt ccacggcaag caagcgcctg ccatgtcttg ttcatccaac
     9481 ccacgtagcg gcagagcacc cacaactgcc gcacatgatc ctgcacacgt ttttggggca
     9541 agcctcctta cgccagctgc tcatcttgtg cgccgagtca taacaatacc tgccagcgtc
     9601 aaggcaattc ccgttattgc aatcggaata tagatgtgat ctgcgatgct taccacaaac
     9661 ctatcaattg tagaaagtac aacatatatc agcacccctg ctgatgtgat gagactcggg
     9721 ttcttcttca ctgctgcttc accttcacca actgcattat ttacgcacaa accaaggaat
     9781 ctgccgcttc agta
//
LOCUS       DAOSYL010000074         9768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_5927_length_9768_cov_8.909297, whole genome shotgun sequence.
ACCESSION   DAOSYL010000074 DAOSYL010000000
VERSION     DAOSYL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5927_length_9768_cov_8.909297"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1553
                     /locus_tag="PLU15_09525"
     CDS             273..1553
                     /locus_tag="PLU15_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO31191.1"
                     /translation="MKRSTWLLAVAIIVSLSFGVGAAKPIELTYWTHTDDNRTEIENR
                     YIAEFTKMYPNVRVKRVVNEASKMGDIVLTAFSANNAPDLFNLPIEQEYGYMKNYRVA
                     PVDCKAIGVKNYDELKSQYIKGTFDAVTMGGKIYGLPLEITNWCIYINKKVFRDAGLD
                     PEKDYPKTWEDMADISDKIAIRNGDILVRRGFDFRYPYYLVSFLPMVEQLGGKLLSDD
                     GKTAIVNDKAWLQVLEYMREWGPHGRNLGSPTYAAPRKIWNKDNNDIAMCLSGFYQQG
                     RLQNDNPAFFESKEWMVVPFPVFKNAVNDVACSFYGHYLMVNAQAPKEKQEMAWKLAD
                     YMLSHSMEYLVKVNLIQPRKELLTSKTFKNLPYTEVFMADMNRAHPVFLHENGYQFER
                     FIKEAVEGVMLSKVPPKEALNTLRNRIQEVLDNE"
     gene            1632..2543
                     /locus_tag="PLU15_09530"
     CDS             1632..2543
                     /locus_tag="PLU15_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31192.1"
                     /translation="MQQHKGFSMEQKKARWGWIFVAPTLAFFLVFSFYPIGNAIYMSF
                     FKKNLLSLKPPEFVGWGNYKYLFSSDMFWQSVANTAIFTVGTFIPLVVLSLVLAAFIM
                     SRTKLQKFLQMALYSPAALSSVVAALIWLLIFDPRGLANQALNFINGTSGVNYHWLSN
                     ANMLRLSTIILYFWKYIGYFTIVFIAGIGGIPDYLHEAALIDGANRWQDFWHITFPLL
                     KPTTLLVSVMVMIQCLKTFSTQYLFTTSGAPTRPIDVITLSIYNTAIKDHNIGRASAM
                     SVLLFLVMLVLTWLQFRISRTEEVTYM"
     gene            2596..3459
                     /locus_tag="PLU15_09535"
     CDS             2596..3459
                     /locus_tag="PLU15_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31193.1"
                     /translation="MNYAKGKAPIGERLADIFIWLFLILACVFVVTPLLFMVTASFMP
                     AIDIMKLPYPWFSDHIHWQNYWNGLKGPDGSFIFVRNILNSLVVALSVTATTVLLSSM
                     SGYGLAKFQFKGRNLVFMLIMATMMIPFETIMIPLYMVVTKMGLHDSYAGLILPFLTN
                     AFGVFLMRQFLITFPDEVLDAARIDGASEPRIFANIVLPNSGPAIATLAILTFRSQWD
                     NLLWPLLVVQSDEMKTIPLYITKFMAETHSDEGIMMAVATMASLPMFILFFTMSNYFI
                     STEGLHSTVKG"
     gene            complement(3569..3745)
                     /locus_tag="PLU15_09540"
     CDS             complement(3569..3745)
                     /locus_tag="PLU15_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31194.1"
                     /translation="MTYFKLATASWLTPIYLLMLAVTVVAVAVNILREEKLSSQIVGG
                     MVLIPLVLRLLLVK"
     gene            complement(3784..5070)
                     /locus_tag="PLU15_09545"
     CDS             complement(3784..5070)
                     /locus_tag="PLU15_09545"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO31195.1"
                     /translation="MWAQLITFVCMLGTFLLFAAALKLPPALATAGAAVVGLAVSGNI
                     GSLYHLIEGAFGYFDVILIIAMAMVFMAALEAAGILEEISYRITRQLGNKPLLLSLAS
                     MVLVMLPGMLTGSSTAAAMTTGRFVLPVLVNAGIAPARAGAFVALGSIIGMIAPPVNV
                     PVMIIGSGIDMPYVGFDIPLLLLSVPAAIAVSIWVVAGINKGPSGDKLEPGQAAPAGA
                     SERIPGFKVYIPIIALLAMMLAVRLSKGSIPDPGIPFMFAAGALLAMVCGRPIKLWDT
                     IVDGIGMAMPVMGVLVGAGMFVQVMTVTGVRGLLVTSALDIPIAWIFVVAALALPLFG
                     AISAYASSSVMGVPILLALLGNDEIVTAAALSMLAAIGDLLPPIAIVPSLISQSSGEG
                     PDFRSELLRQAAVPIFCLVLWAFAVLRWAPAIGRLL"
     gene            complement(5151..6872)
                     /gene="ggt"
                     /locus_tag="PLU15_09550"
     CDS             complement(5151..6872)
                     /gene="ggt"
                     /locus_tag="PLU15_09550"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOO31196.1"
                     /translation="MFRPDSRKRIFAIVALLALVMVMTGVAGAELPQEVRAPNGMVAA
                     AHQLAAEAGVEILKAGGNAVDAGVATAFAVGVVEPNASGLGGEGMIVIYLPEGDRAVV
                     IDYRSTAPAAAAEALADSKMPGTGWPSVATPGLVAGLASALEQYGTMTLEQVLQPAIR
                     LAEEGFPIGATLAGVIEDRYETVLNDPELAATFLDDGLPPQEGWILKNPGLASSLKKI
                     AAEGPDAFYRGEIAQAIDAASRAGGGYITAEDLANYKAVTRWPARGNYRGYEVFSAPP
                     PVGGATLIEVLNIVENFDLSAAPFPNAQAIHYLSESIKPAFRDYRAYVHDPDFVYVPL
                     QEMLSKDYAKERASKVNPDKMTPAGKIQAGEFAKKAALAPTGTEGAKYESPSTTHISV
                     IDKDRNMVAITQTISSFFGAGVMIEGTGVILNNEMANFSTDKTSGNYIAPNKRMRTSI
                     APTLVLREGEPFLSIGTPGAGRIVPTMAQLLVNIIDFDMGLQEAINAPRFYAREGSDV
                     FEYEPRFPEEVIDDLKKMGYPIDEKSVRGEYDLYFGGAQGVMVDPNTGELIGAADPRR
                     DGAAVGY"
     gene            complement(6901..7944)
                     /locus_tag="PLU15_09555"
     CDS             complement(6901..7944)
                     /locus_tag="PLU15_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOO31197.1"
                     /translation="MRRMSRTVCAVFAAALVMAVAVAPALAEPIIHDVRPGYGVTGIG
                     WISDYHAPLRGSAVDTRVYYLDSKVEGPTTLVLGGTHSNEIAGIMAATLLVERAVVTT
                     GRLIVVPYANASGASYADTYHPEIGYYSLTTESGVRVFRYGDRLTNPEHQGPDADKYI
                     HYPSGQEFPGYEARNLDRAHPGKADGNLTQQLAYAYHQLLLEEKVDVAFDLHEAGPTS
                     RLANMVVANPKNIDFAVMAVVDLEMQGIVMNVEHSSDSFHGLSHREWGDTTPAASYLI
                     ETPNPAQAPNAINPDVVNDKVNPLSRRTATHLATIDAVLNAHELLDGKRTRWVGLPSY
                     EELVSDGLEEYLR"
     gene            complement(7974..8549)
                     /locus_tag="PLU15_09560"
     CDS             complement(7974..8549)
                     /locus_tag="PLU15_09560"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOO31198.1"
                     /translation="MDGRMSNLRFVAVVVIAALVLTPVLAVAAPKPGKPALITSAGQS
                     TDGLILKTVMTDKATGETIPFEKLAKPEDLKGIRTLIVSVGLSSKGLGAAGVNVDQEK
                     ARVRSILDAAKKNDIFVIMVHIGGTARRGAGSDEFATLVADYAHHIFVVRAGNDDGFF
                     TRLAQKNKIPLTEVDDRIAMGPAIMKLLNTK"
     gene            complement(8570..9766)
                     /locus_tag="PLU15_09565"
     CDS             complement(8570..9766)
                     /locus_tag="PLU15_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOO31199.1"
                     /translation="MEQRIWSAQSLLTMDTEVKPMGDRGARGVVVIVIALIVTAVSAM
                     RFAPLGPEELEAYPSPSISRVAKAKLSAYNSALLGTAGDTEVLIYEGSQPGGTMLVVG
                     GTHPDEPAGTVAALILAENVVVVQGKLIVIPYANASGFTHNLPQEGHPPYYTLSTPGG
                     DRIMKYGSRLTNPVHQWPDPTVYVEKVSYQKLAGTEARNLNRAYPGEKNGSLTERVAY
                     AITRLIIDEEIDVSIDLHESSPEYPVNNAVVAHERAFDLAAIATLDLEMEGVQINVEP
                     SPPNLRGLSHREWGDNTDTLALLFESPNPVQGRLRGVTDADLIVDGVDPMYVKASERG
                     RLYVPYTSEGTPLAMRAGRHLASVQAIAAAYSMMAPDRAIVMAGLPSYGEIVANGAGA
                     YLNPSR"
BASE COUNT         2290 a         2751 c         2435 g         2292 t
ORIGIN      
        1 agctgagata ctttattatc atcaagtccc ccaggcggca caaggtctat gccacgccag
       61 gcgccttcac ttactgacta acatatagca aaaacgtgtc ggatttgcag ttggacgcag
      121 gactacttac ttcaatgacg aattttgttt atagttggtt tctaatgaca acgatcacgc
      181 tggggtttcc tgtagtcctg gcatggaggt gacgcgccgc agacactaca tgggttggct
      241 gcccaacaaa gacgctagag gaggagttgt tcatgaaaag gtctacatgg ttgctcgctg
      301 ttgccatcat cgtatccctg tccttcggag tcggcgccgc aaagcctatt gagttgacct
      361 attggaccca cactgacgac aaccgaaccg agatcgaaaa caggtatatc gctgagttca
      421 ccaagatgta ccctaacgtg cgcgtcaaga gagtcgtaaa tgaagctagc aagatgggag
      481 acattgtcct taccgctttc tcagccaata acgctccaga cctattcaac cttccaatcg
      541 aacaagaata cggctacatg aagaactacc gcgttgctcc tgtcgactgc aaggccattg
      601 gcgtcaaaaa ctacgacgaa ttgaagtccc agtacatcaa aggcaccttt gatgcagtga
      661 ccatgggcgg gaagatatac ggccttcccc tagagatcac taactggtgc atctacataa
      721 acaagaaggt cttccgcgat gcagggctcg atcccgagaa agactacccc aaaacatggg
      781 aagacatggc cgatatctcc gacaagattg ccatacgcaa tggtgatatc ctcgtgcggc
      841 gcgggttcga cttccgctat ccgtactacc tcgtgtcttt cctccccatg gttgagcagt
      901 tgggtgggaa gttgctgagt gatgacggca agacagccat agtcaatgac aaggcatggc
      961 tgcaagtgct ggaatacatg agggaatggg gtccccacgg acgcaacctc ggcagcccca
     1021 catacgccgc tcccaggaaa atctggaaca aggacaacaa cgacatagcc atgtgcctca
     1081 gcgggttcta tcagcaaggc cggttgcaga acgataaccc tgcattcttc gaaagcaaag
     1141 aatggatggt tgttccgttc ccagtcttca agaacgccgt caacgatgtg gcgtgctcct
     1201 tctatggcca ttacctgatg gtcaacgccc aggcgcccaa agaaaagcag gagatggcgt
     1261 ggaagttagc tgactacatg ctcagccatt ccatggagta tctggtaaag gtcaacctca
     1321 ttcagccgcg caaagagctc ctgacgtcga agacgttcaa gaaccttcct tacactgaag
     1381 tcttcatggc agacatgaac cgggcacatc cggtcttcct ccatgaaaac gggtaccagt
     1441 ttgaaagatt tataaaggaa gcagttgaag gcgttatgct gtccaaggtg cctccaaaag
     1501 aggcccttaa taccctgcgc aaccgtatcc aggaagtcct ggataacgaa tagcagtagc
     1561 agcgcaagct gaagtccggg ttcttgcaac ccggacttta gcttccgaga tttccttgag
     1621 ggggagattc gatgcagcag cacaagggat tcagtatgga acagaaaaag gccagatggg
     1681 gatggatatt cgtcgctccg actttggcgt tcttcttagt cttcagcttc taccccattg
     1741 gcaatgcaat ctacatgagc tttttcaaga agaatctgtt gtcgctcaag ccgcctgaat
     1801 tcgtcggttg gggcaactac aagtatttat tctcgtcaga catgttctgg cagtctgtgg
     1861 ccaatacagc catattcaca gtgggcacat tcattcccct ggtcgttctc agcctcgtat
     1921 tggccgcttt catcatgtcg cggacaaagt tgcagaaatt ccttcagatg gcgctatact
     1981 ccccggccgc gctttcgtcg gttgtggcgg cccttatctg gctgcttatc ttcgatcctc
     2041 ggggcctggc aaaccaagcg ctcaatttta ttaatggaac atcgggagtc aattatcact
     2101 ggctctctaa cgcgaacatg ctgcggttgt caacgataat actgtatttt tggaaataca
     2161 tcggctattt caccatcgta ttcattgctg gcataggagg catcccagac tacttgcacg
     2221 aggcagctct gatcgatgga gcaaacagat ggcaagactt ctggcatatc acatttccgc
     2281 tactgaagcc gaccactctc ttggtttcag tgatggtcat gatccagtgt ctcaagactt
     2341 ttagcacgca atacctcttc acgacatccg gggcgcccac cagacccatt gatgtcatta
     2401 ccctcagtat ctacaatact gcaatcaagg atcacaatat tgggagagcc agcgccatga
     2461 gtgttctgct tttccttgtc atgttggtcc taacatggtt gcaattcagg atatctagga
     2521 ctgaggaagt cacttacatg tagaaatatc aaaacagggc gatcagctag atgctctggg
     2581 atcaggggga ttgacatgaa ttatgctaaa gggaaggccc caatcggaga aaggctggca
     2641 gacatcttta tctggctatt tctcattttg gcctgcgtgt tcgtggtcac accacttctg
     2701 ttcatggtta ctgcatcctt catgcccgca atagatatta tgaaactgcc ctacccttgg
     2761 ttcagtgatc acatacactg gcagaactat tggaatggtt tgaagggacc tgacgggtca
     2821 ttcatatttg ttcgcaacat actcaactct ctcgtcgttg ccctttctgt caccgcaaca
     2881 acagttcttc tgtcgtcaat gtcaggatat gggctcgcta aattccaatt taaaggtcgc
     2941 aatctcgtct tcatgctgat aatggcgaca atgatgatcc ctttcgagac catcatgatc
     3001 ccgctctaca tggtagtcac aaaaatgggg ctgcacgatt catatgccgg actcattctg
     3061 cccttcctca ccaacgcttt tggcgtgttt ttgatgcggc agtttctgat caccttcccc
     3121 gatgaggtgc tcgatgccgc ccgcatcgat ggcgcgagtg aaccccgcat tttcgccaac
     3181 atcgtgttgc ccaacagtgg tccggccatc gccaccctgg cgattctgac gttccgctca
     3241 caatgggaca acctgctctg gcccctgctc gtggttcaga gcgatgaaat gaaaacgatt
     3301 cctctgtata taaccaagtt catggcggaa acccacagtg acgaagggat catgatggcc
     3361 gttgcgacca tggccagcct tccgatgttc atcttgttct tcactatgtc gaattacttc
     3421 atcagcaccg agggcttgca ttccacagtg aagggttgaa catcaagaac ccgcgccgcg
     3481 ccttcacacg gccgaacgcg ggttcttccc ttcggggata cagtagaggg atggacgtta
     3541 atcttgcact cctccgaaat cctcgcgctc acttcaccaa aagcagacgg agcacgagag
     3601 ggatcagcac catgccgccg actatctgcg atgatagttt ctcttcccgc agtatgttga
     3661 ctgcaacagc cacaaccgtc accgccaaca tgagaagata tatcggcgtg agccaacttg
     3721 ctgttgccag tttgaagtag gtcattctat cgtttcctcc tctagcgtcg cgttccgcct
     3781 aagctacaac agtctgccta tggcaggggc ccatctcagc accgcaaagg cccagagcac
     3841 gagacagaat ataggcaccg ctgcctgcct cagcaattca gatcggaaat cgggcccttc
     3901 tccagatgat tgggatatga ggctgggcac tattgcgatg ggcggcagca gatcgcctat
     3961 cgccgccagc attgaaagag ccgcagctgt tactatctcg tcgttgccaa ggagcgcgag
     4021 gagaatcggc acgcccataa cggaagatga cgcataggcg gagatcgccc cgaaaagcgg
     4081 tagggccagt gcagccacta cgaatatcca cgctatcggt atatccaagg cggatgttac
     4141 aagcaaccct ctgacgcctg tcacagtcat cacctgaacg aacatgcccg cgccaacaag
     4201 cacgcccata acaggcatcg ccatgccaat tccatcgact attgtgtccc agagcttgat
     4261 cggccttccg caaaccatgg caagcagcgc ccctgcggcg aacatgaatg ggatgcccgg
     4321 atcgggtatg gagccctttg acagcctaac cgcgagcatc atcgccaaaa gtgcgataat
     4381 gggaatgtaa actttgaaac cgggaatgcg ttcactggcg cctgccggcg cggcctgtcc
     4441 cggttctagc ttgtcgcctg agggcccctt gttaatccct gccaccaccc atattgagac
     4501 agctatcgcg gcgggcacgg aaaggagcaa caacgggatg tcgaagccca cgtagggcat
     4561 gtctattccc gacccgataa tcatcaccgg cacgttcaca ggaggcgcaa tcatcccgat
     4621 tatgctgccg agagcgacaa atgcgcctgc ccgagccgga gcaatgccag cattgacgag
     4681 cacaggcaag acaaaccttc ctgtggtcat agcggcagct gtggatgaac cggtcagcat
     4741 gcccggaagc atcaccaaga ccatggacgc caaagagagc aacagaggct tgttgccgag
     4801 ctgtctggtg attctgtagg agatctcttc caggattcct gctgcctcga gcgcggccat
     4861 gaacaccatt gccatggcga taatcagaat cacatcgaag tagccgaaag ccccttcaat
     4921 cagatgatac aaggacccta tgttgcccga aacagcaagg cccactacag cggcgcccgc
     4981 tgtagccagg gctggaggca gcttgagcgc tgcagcaaac agcaaaaacg tgcctagcat
     5041 gcagacaaat gtaatgagct gagcccacat tagcatcact cttccatgaa aaaggcgcga
     5101 cgttcggcgt cccgaccgcc gcgcccggtt ttgtttgctt ccttatgtcc tcagtaacct
     5161 acagcagcgc catcccgcct tgggtcagca gctccaatca gttcgccggt gttggggtcg
     5221 accatgacgc cctgagcgcc tccgaaatac agatcgtatt cacctcgtac cgacttctcg
     5281 tcgatgggat aacccatctt cttcagatca tcaataacct cttcagggaa gcggggttcg
     5341 tattcgaaca cgtcagaacc ctctcgcgca tagaatctgg gtgcatttat ggcctcttgc
     5401 agccccatgt cgaagtcgat tatgttgacc agcagctgcg ccattgtagg cacgatcctt
     5461 ccggcgcccg gagttccgat gctcaggaag ggctctccct cgcgcagcac gagcgttgga
     5521 gcgatcgaag tcctcatcct cttgttggga gcaatgtagt tgccactggt cttgtcagtt
     5581 gagaagttgg ccatctcgtt gttgagaatc acaccagtgc cctcaatcat tacgcctgct
     5641 ccgaagaatg agctgatagt ctgggtgatc gccaccatgt ttcggtcttt gtcaattact
     5701 gaaatgtgag ttgttgacgg tgattcgtat ttggcgcctt cggtgccagt tggagccaga
     5761 gcagccttct tggcaaattc gccagcctga atcttgccag ctggggtcat cttatcgggg
     5821 ttgaccttgg aagcgcgctc ttttgcataa tccttagaga gcatctcctg caaaggcaca
     5881 tacacgaaat caggatcatg cacgtatgca cggtaatcac ggaacgcagg ctttattgac
     5941 tccgaaaggt agtgaatcgc ctgggcgttc gggaacggag ctgcgctgag atcgaagttc
     6001 tccacaatat tcagaacttc aatcaaggtt gcgcctccta ccggtggcgg cgcagagaac
     6061 acctcgtatc ctctgtagtt gcctcgtgca ggccatctcg ttacggcctt gtaatttgcc
     6121 aagtcttcag cggtgatgta gccgccgccc gctcggctgg cagcatcgat cgcctgcgca
     6181 atctccccgc gatagaatgc gtcggggcct tctgcagcga tcttcttgag cgatgacgcg
     6241 agaccggggt ttttcagaat ccagccctcc tgcggaggca gaccatcgtc aaggaatgtc
     6301 gcagcaagct cagggtcatt cagtacggtc tcgtatcggt cttcaatgac gccggcgagg
     6361 gttgcgccaa tagggaatcc ctcttcagcc agcctaattg caggctgcaa tacctgctca
     6421 agcgtcattg tcccgtactg ctccagagcc gaagccaggc ccgcaacgag cccgggggtg
     6481 gccactgacg gccagcccgt ccctggcatt ttgctgtctg ccagggcttc agccgcagcc
     6541 gcaggcgccg tcgacctgta gtcgatcaca acggccctgt ctccctcagg aaggtagatt
     6601 acgatcatgc cctcgccgcc taagcctgat gcgttgggct ccactactcc gactgcgaac
     6661 gcagtggcaa ctccagcatc aacagcgttt ccaccagctt tgaggatttc aactccggct
     6721 tcggccgcca actggtgcgc cgcagcaacc ataccgtttg gcgcgcggac ttcctgtggc
     6781 agctccgccc ctgccacacc agtcataacc ataaccaacg caagcagtgc cactattgca
     6841 aaaatgcgtt tcctggaatc aggtctgaac atagtctgtg ccctccttct taattccggt
     6901 ctatctcaga tattcttcga gtccgtccga aaccaattcc tcataagaag gcaatccaac
     6961 ccaccgcgtg cgcttgccgt caaggagctc gtgtgcattg agcactgcat ctatcgtagc
     7021 aaggtgtgta gcagttctgc gggacaaggg attaaccttg tcatttacca catcagggtt
     7081 gatggcattg ggggcttggg cagggttagg cgtttcgatc aaatacgaag cagcaggagt
     7141 agtgtctccc cactccctgt gggaaagacc gtgaaaactg tcggacgaat gctccacatt
     7201 catcacaatt ccctgcatct ccagatcgac tacggccatc accgcgaaat caatattctt
     7261 ggggtttgcc acaaccatgt tcgccagtcg agacgtgggg ccggcttcat gcaggtcgaa
     7321 agccacatct accttttcct caagcaacaa ctggtggtaa gcgtatgcaa gctgctgggt
     7381 gaggttgccg tcagccttgc ccggatgggc cctgtcaagg ttgcgcgctt cataaccggg
     7441 aaactcctga cccgacggat agtgaatgta cttgtcagca tcgggtccct ggtgctccgg
     7501 gtttgtgagg cgatctccat acctgaaaac acgaactcca ctctctgtag tcaagctgta
     7561 ataaccgatc tcgggatgat atgtatctgc atagcttgcc ccggatgcgt tggcgtatgg
     7621 gactactatg agtcttccgg tagtaaccac cgccctctcc acgagcaatg tggcagccat
     7681 gatcccggca atctcgttgg aatgagttcc tcccaacaca agggtcgtgg gcccttccac
     7741 tttgctgtcc agatagtata cacgggtatc cacagcagaa ccgcgcaacg gggcgtggta
     7801 atcggatatc catccgattc ccgtaacccc atatccaggc ctcacatcgt gaattatcgg
     7861 ctcagccaga gcaggcgcta cagccactgc catcaccaga gccgccgcga aaactgcaca
     7921 gaccgtccta ctcattctgc gcatctaata ccctccagac acatgctctc atgttacttg
     7981 gtgttaagca gtttcatgat tgcgggcccc atggcaatcc tgtcatccac ttctgtcagg
     8041 ggaatcttgt ttttctgcgc taaccgggtg aagaatccgt catcgtttcc cgcccggacc
     8101 acgaaaatgt ggtgtgcata atcggcaact agtgttgcga attcgtcaga ccctgcgcca
     8161 cgacgagcgg tgccacctat gtgcaccata atcacgaata tgtcgttctt cttagccgca
     8221 tcaagaatcg aacggactct agccttctcc tgatcgacgt tgacccctgc ggccccaagg
     8281 cccttgctgc ttaggccgac tgacacaatc agagttcgaa ttcctttgag atcttcgggc
     8341 tttgcaagtt tctcgaaagg aattgtctcg cctgtagcct tgtcggtcat aactgttttc
     8401 agaatcagac cgtcagtgct ctgccctgca gatgttatta gcgccggttt gccgggtttg
     8461 ggcgcagcaa ctgcaagtac aggagtcagc acgagtgccg cgataacaac tacagccaca
     8521 aaacgaagat tgctcattct cccatccatg tgttgttctc cttccttctt tatctgcttg
     8581 ggttcagata ggctcctgcg ccatttgcaa ctatctcgcc atacgacggc aaccccgcca
     8641 taacaatggc tctgtcaggc gccatcatgg aataggccgc tgcaatagct tgcactgacg
     8701 caaggtgcct gcccgccctc attgcaagcg gagttccttc acttgtatat ggcacataca
     8761 aacgtcctcg ctcagatgcc ttcacgtaca taggatccac cccgtcaacg atcaaatcag
     8821 catctgttac tcccctcagt ctgccttgga cggggttagg tgattcaaac agcagcgcca
     8881 acgtatcggt attatctccc cattccctgt gcgaaagccc cctcaggttg ggtggagacg
     8941 gctcgacgtt gatctgcacg ccttccattt ccaaatcgag agtagcaata gctgccaggt
     9001 caaacgcccg ctcgtgggca acaacagcat tgttcacagg atactcggga gatgattcgt
     9061 ggaggtcgat ggagacatct atctcttcat cgatgatgag ccgggtgatg gcatatgcca
     9121 cgcgttccgt cagcgagccg tttttttcgc cggggtaagc cctgttgaga ttgcgtgcct
     9181 cggttccggc aagtttctga taggatacct tttcaacata gaccgtggga tcaggccatt
     9241 ggtggacggg attcgttaaa cgcgaaccat atttcatgat tcgatcgcca cccggggtag
     9301 atagggtgta gtatggggga tgtccctcct gaggaaggtt gtgggtaaag cctgaagcat
     9361 ttgcataagg tatcacaatc agcttgccct gcaccacgac aacgttctct gccagaatca
     9421 gagctgcaac cgtgcccgct ggctcatcag gatgagttcc cccaaccaca agcatcgttc
     9481 cgcctggctg agagccctca tagatcaata cctcagtatc tcctgcagtg cccaaaagtg
     9541 ccgagttata tgcagatagc ttagctttag ctacacgact gattgaaggc gatgggtacg
     9601 cctctagttc ttcagggccc aatggcgcaa acctcattgc agataccgct gtcacaatca
     9661 aagcaatgac gattacgact acccctcttg cgcctctgtc gcccatgggt ttcacctccg
     9721 tatccatagt caacaggctt tgcgcgctcc atattcgttg ttccatat
//
LOCUS       DAOSYL010000075         9609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_6068_length_9609_cov_8.613984, whole genome shotgun sequence.
ACCESSION   DAOSYL010000075 DAOSYL010000000
VERSION     DAOSYL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6068_length_9609_cov_8.613984"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..513)
                     /locus_tag="PLU15_09570"
     CDS             complement(316..513)
                     /locus_tag="PLU15_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31200.1"
                     /translation="MSAPTLPVLGVIEIQRTTAKHAYNEDGNEPAPRFGKPVTDHVAD
                     IVDIWLLEDQLLSRKERRNTR"
     gene            complement(804..1445)
                     /locus_tag="PLU15_09575"
     CDS             complement(804..1445)
                     /locus_tag="PLU15_09575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOO31201.1"
                     /translation="MEIRVSPTRMQLLQLKKSHAMAVRGHKLLKDKLDELMKQFLELV
                     KRDRELREEVDEKLSEAFRGFLIARGAMSSSAVDAALMYPKEQISLKASRRHIMNIEV
                     PEFEWSFEGQEDRTNIYPYGFAYTSPELDTAIRLLSEAMPSLVELAEVEKSVELLAGE
                     IEKTRRRVNALEHVVIPQQEASIKYITMKLDEAERANITRLMKVKEIVGERSV"
     gene            complement(1454..2857)
                     /locus_tag="PLU15_09580"
     CDS             complement(1454..2857)
                     /locus_tag="PLU15_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOO31202.1"
                     /translation="MLKEYLTVREVAGPLMLVEKVDEAKYGELVEIVVGGGEIRRGRV
                     LEVEGTNALVQLFEGSAGLELTSARVRFLGRGLELGVSPEMLGRVFDGLGRPMDDGPK
                     IIPEARLDTNGSPINPYARDYPSEFIQTGISAIDGLNTLVRGQKLPIFSGAGLPHSQL
                     AAQIARQARVLGTGEQFAVVFGAMGITFEEADFFISDFRRTGAIERAVLFINLADDPA
                     IERISTPKMALTAAEYLAYELDMHVLVILTDMTNYCEALREVSAARKEVPGRRGYPGY
                     LYTDLATLYERAGRIKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIIVSRELH
                     RKGIYPPIDVLPSLSRLKDKGIGEGKTREDHADTMNQLYAAYARGKEAKELAVILGEA
                     ALSPSDKIFSKFADRFEQEYVRQGVDENRSIEETLDIGWDLLAMLPVDELSRIRMEFI
                     EKYLPKEAEKDAEPARE"
     gene            complement(2850..4634)
                     /locus_tag="PLU15_09585"
     CDS             complement(2850..4634)
                     /locus_tag="PLU15_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO31203.1"
                     /translation="MNRGTSVGTIVKVSGPLVVASGMAGAKMYDVVRVSEQRLIGEII
                     EMREDRASIQVYEETAGLGPGEPVYITGEPLSVELGPGLIGSIYDGIQRPLDRIRDMA
                     GDRISRGLEAPGLDREREWDFVPRAGVGSKVVPGDILGTVQETPVVEHRVMVPPGISG
                     KVIWIHKGVAKVTDVIAKIETSDGVREIQMMQRWPVRHGRPIAHKLTPSEPLVSGQRV
                     LDTFFPVAKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYVGCGERGNEMTDVLMEF
                     PELLDPQSGEPLMKRTVLIANTSDMPVAARESSVYTGITIAEYFRDMGYSVALMADST
                     SRWAEALREMSGRLEEMPGEEGYPAYLGSRVSEFYERAGRTTCLGSDGREGALTVVGA
                     VSPPGGDLSEPVTQTTLRVVKVFWALDANLAYSRHFPAINWLTSYSLYLDRIDESLRK
                     MVGSEFVEMRQEAMKILQEEASLSEIVRLVGMDALSPGERITMETARSIREDFLHQNA
                     FHDVDTYTSLKKQAAMLRVILEMDHACRDAVEKGASFGQLAELAVHGKIARMKYIEEA
                     KISEIDELALEVRRDVAALTAGGGEDVA"
     gene            complement(4636..4965)
                     /locus_tag="PLU15_09590"
     CDS             complement(4636..4965)
                     /locus_tag="PLU15_09590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOO31204.1"
                     /translation="MHKIAVIGDEDSILGFRALGVSVFPAKNGKEAEEALRAAARRDY
                     AVIYITETHASEIIDVITEYSQAPLPVVLTIPDTRGGQGAAMEKIRRTVEKALGADIL
                     FGREGNA"
     gene            complement(4958..6070)
                     /locus_tag="PLU15_09595"
     CDS             complement(4958..6070)
                     /locus_tag="PLU15_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOO31205.1"
                     /translation="MGFDDRFAYVSGRIWALEDRMVDRAKFNRLIDAGSPEELVRILS
                     ETEYGQGREIVPERYEEIIDAELVRVHQLIAELSPDPELTGVFRAKHDFHNMKVHLKS
                     RLVKRDRDMIVREGLGLSQVGWIDPDRLGEYADAIMLEYYPVDEEEEDDPGERDDFVA
                     FDGSDEILATTPLEDAIIDAGHKAVEAYMRHGRDPQYIDFVIDRASYDYFHHVAVSRR
                     AVRLEEIVATMADLTNIQICMRLRAIGKDACFASDVLVPHGTLDAGALLGVYEKPDSE
                     FVAYFRDTPYARLVEDAMNAWLANGSLVALENLAEGYLFGKIAPEGEAVVGYETALAY
                     LGARELEADKLRRIFVGKMNKIPPNVIRERLSGAYA"
     gene            complement(6073..6678)
                     /locus_tag="PLU15_09600"
     CDS             complement(6073..6678)
                     /locus_tag="PLU15_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOO31206.1"
                     /translation="MENLERLKGRILDEAREKADRILDAADERCAEIQREAEAKSDEI
                     RKRAVERARANAREEERRAEIMRSLDVRNAMLRAKGDAVDKLMAEIPATIRALPDEEY
                     LGIMKRMLINAAPAGQVELVVALPDRGRIGSDFVKEAASELVAAGKQTKLKLSTETAD
                     LTGGFILRASTVEVDCSLDSLLALCEDELAPLVAEALFGRS"
     gene            complement(6698..7177)
                     /locus_tag="PLU15_09605"
     CDS             complement(6698..7177)
                     /locus_tag="PLU15_09605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOO31207.1"
                     /translation="MDFTLGTLLGFMAIGLAVIPGGYASSKAVGMVGRAGAGVITEDP
                     DKFGSILILQALPGTQGVYGFLTGLSIMVKIGVLGGSIQALTVNQGIMFILAAVPVAA
                     LGWLSAIFQGQVAASGVSIVAKRPEEMGKAVILAAMVETYAVLALLASLLMVLSINV"
     gene            complement(7183..9165)
                     /locus_tag="PLU15_09610"
     CDS             complement(7183..9165)
                     /locus_tag="PLU15_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOO31208.1"
                     /translation="MSRVTVLGHESRIGDVVDVLQQLGTAEVSQFGEDLADEELLDLA
                     CPYDASERIAELDSILAELSYTIDMMGKHRHIKRDIVQNFSGFRLYMTEDEWNAYAEG
                     GAEAARDIYSRAKAIEDTLAQLATNEASILTSIEILMPWQGLDAPIEEIRTGRHVELV
                     LGTADARKLEASLAEASEIVEGLAWELVGVDGERAYLLTAYPLSEAEEAARALSQAGF
                     AEVRLDQYSGVPRDIIARLRKELDSIYRRRDQIEQEIDALVEDRPRVFVLHDHFYLER
                     EKYAITNRFGGTEHTFMVEGWVQTKDLEELRAEIEGIADDIVVLDRPRFEDEEAPVEL
                     SNKPLVAPFEVITKTMGYPKQGTIDPSPYLGPFFLVFFGLCMTDAVYGIFIALVAFQL
                     MKKTHTRPGEGGFLDLIAMCGFATIFVGALLGGWLGDLPKRLFGWDTAILFDPMESPT
                     TFLILSFVMGIIHLYWGIIIKGIDNVKKGEWLSAIYDQGFWLVWLTSLMLTLGATALG
                     PNGPAIAAVSKWFLIASAIGLIATQGRQHKNPIKRIGAGLISLYNTTGFMSNVISYAR
                     LFGLGFTSTVLASVMNDLFLRPAGIPVVGPVIAVVGLAFGHLFNILINIIGSYVHSSR
                     LQYVEFFGEFFEAGGRRFMPFGMSTKYVTVENSKEA"
     gene            complement(9167..>9609)
                     /locus_tag="PLU15_09615"
     CDS             complement(9167..>9609)
                     /locus_tag="PLU15_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31209.1"
                     /translation="DFFYRCTRPCICSIIPDESMQQCVVHGSFLLDVGVLLALSIEAI
                     DEIRDVESQADEIVRAAIEEARARVAAAEERARESVVEAERSAMAEAARVEDESRART
                     AAEADKAKKQVESKVKELDGIARSNWDKALKLAAGKVLSSIGDL"
BASE COUNT         2156 a         2975 c         2257 g         2221 t
ORIGIN      
        1 gccttcagag ccatcaagtc ggaggtgaag aagctggcga aatccaccat ggatgaatcg
       61 aactagcaca gcctgtgagg actctgacca agccaacgaa gaaacagatg gctattctca
      121 atgtcttggc gttgttagcc cagcccagat caacccgata ctgcagagcc agaactacat
      181 cgactcagat cctccggcaa caagcgccaa ctcaagcgtg tagtccaaag gtctcgccgt
      241 attgacagtc aagccccctg ataagagggt acttacgttt cgcgcgacgt caaactcgag
      301 atgcaagaac aatcgtcatc tagtatttcg ccgttccttt ctcgacagca actgatcctc
      361 aaggagccag atatctacaa tgtctgctac gtgatcggtt acaggctttc caaacctggg
      421 cgctggttca ttcccgtcct cgttgtaagc atgttttgcc gtcgttctct gtatttctat
      481 tacgccgaga acaggcaaag taggtgcgct caacccatgg cctccctcca catggagcta
      541 aggtccttca ggaaggcaac ctctgcggct agccgatatc tgcccctcat gccacaactc
      601 accaacaaaa cgaaaacgcc agaacgcaca tgcgccccgg cgtctacata ccgtcattgg
      661 ctaacctcta ccagatcacc aattacgtct ctgaacgatt cccgagtttg cattgcctgt
      721 agtttaaacg tttagctgct cagtcaggct gagatacttt ttgtcggtcg cttgcagggc
      781 aaggctaacc agcgagcagt cgatcaaaca ctccgctcgc ctactatctc cttaaccttc
      841 atcagtctgg taatgttggc ccgttccgcc tcgtcgagct tcattgttat atatttgatc
      901 gaagcctcct gctgcgggat gacaacgtgt tcaagagcgt taactcttct ccgggtcttc
      961 tcaatctccc ccgccaggag ctctactgac ttctccactt cagccaactc caccaggctc
     1021 ggcatggcct cggaaagaag cctaatagcc gtatcgagct ctggagaggt gtaggcgaac
     1081 ccatagggat agatgttcgt cctgtcctct tgtccctcga aactccactc gaactcggga
     1141 acctcgatat tcatgatatg cctccgagaa gccttcagag atatctgctc ctttggatac
     1201 atcaaggccg catccactgc gctggatgac atcgcgcccc gcgctataag aaagcccctg
     1261 aaagcctcgc taagcttctc atctacttcc tctctcagtt ccctgtcccg cttgaccagt
     1321 tcgaggaatt gcttcatcag ctcatccagc ttatctttga gcaatttgtg cccacgaaca
     1381 gccatggcat ggctcttttt gagctggagc agctgcatcc ttgttgggct gactctgatc
     1441 tccatagccc cgatcactcc ctcgcgggct ctgcgtcttt ttcggcttct ttcggcagat
     1501 acttctcgat gaactccatc ctgatgcggc tcagctcatc cactggcaac atcgccagca
     1561 gatcccagcc tatatcgagc gtctcctcaa tgctccggtt ttcatcgaca ccctgcctca
     1621 catactcctg ctcgaagcga tcagcaaact tcgagaaaat cttgtcagag ggagacaaag
     1681 ctgcttcgcc caggatgact gctagctcct tggcctcctt gcccctggca tacgccgcat
     1741 aaagctggtt catggtgtcg gcgtgatcct ctcttgtctt gccctctccg attcctttgt
     1801 ccttcaacct cgatagtgaa ggcaatacgt caatcggcgg atagatcccc ttcctatgga
     1861 gctccctgct gactattatc tgaccttcgg tgatgtagcc tgtcaggtcc ggaatggggt
     1921 gagtcttgtc atcttcaggc atggttaaaa tcgggatctg cgtaatcgat cccttcttcc
     1981 cttttattct gcctgcacgc tcataaaggg tggctaggtc tgtgtaaaga tacccaggat
     2041 acccacgcct cccggggacc tccttgcggg ctgccgacac ttcccgcaat gcctcacagt
     2101 agttggtcat gtcagtcagg atgaccaaca cgtgcatgtc aagctcgtac gcgagatatt
     2161 cagcagctgt cagcgccatc ttgggcgtcg aaatccgttc gattgcagga tcgtctgcta
     2221 ggtttatgaa aagaacggca cgctcaatgg cacccgtacg cctgaagtca gagatgaaga
     2281 aatcggcctc ttcaaaggtg atgcccatgg ccccgaaaac gacggcaaac tgttcccctg
     2341 tccctaacac tctagcttgc cttgcaatct gtgcagccaa ctgcgagtgg ggcaagcccg
     2401 cgccggagaa tatgggcagc ttctgcccgc gcacgagcgt gttcaagccg tcgatggcag
     2461 atattcctgt ctggatgaac tcagatggat aatccctggc ataggggttt atagggctgc
     2521 cgttcgtgtc gagcctggcc tctggtatga tcttgggccc atcatccatt ggccgtccca
     2581 atccatcgaa gacgcgtccc agcatctcag gggacacgcc taattcgaga cctcgcccca
     2641 gaaatcggac ccgggccgac gtaagctcca gaccggctga gccctcaaaa agctgcacaa
     2701 gagcgttggt tccttcaacc tcgagcacgc gtccgcgcct gatctcgccg cccccaacta
     2761 ctatctcaac cagctcgccg tatttagctt catcaacctt ttcaacgagc ataaggggcc
     2821 cggcgacttc tctgacagta aggtattcct taagcaacgt cctcacctcc gccggcggtc
     2881 aaagcagcta cgtctcgtct gacttcgaga gccaactcgt caatttccga gatcttagct
     2941 tcctcaatgt acttcattct cgcgatcttt ccatgcactg ccagctccgc caattggcca
     3001 aacgaagcgc ccttttcgac cgcatcacgg cacgcatgat ccatctccag tatcaccctt
     3061 agcatcgccg cctgtttctt cagggacgtg taggtgtcca catcgtggaa agcgttctgg
     3121 tgcaggaagt cctcgcggat gcttcgcgcg gtctccatgg ttatgcgttc tccgggcgac
     3181 aacgcgtcca tgcccaccag cctcacgatc tccgaaagac tcgcctcttc ctgaagtatc
     3241 ttcatggcct cctggcgcat ctcgacaaac tcgctgccca ccatcttcct aaggctctcg
     3301 tcaatcctgt ccagatagag cgagtagctt gtgagccagt tgatcgccgg aaagtggcgg
     3361 ctataggcca ggttcgcatc gagggcccag aaaaccttca caaccctcaa agtcgtctgg
     3421 gttaccggtt ccgacaggtc tcctcctgga ggtgatactg cgccaaccac tgtgagggca
     3481 ccctctctgc catctgagcc aaggcacgtt gttctgcccg ctctttcgta gaactccgat
     3541 accctggagc ctaggtatgc cggatatccc tcttcacctg gcatttcttc aagacgccca
     3601 gacatctcgc ggagcgcctc agcccatctt gatgtagagt cggccattag ggcgactgag
     3661 taccccatgt cacggaagta ctcggcaata gttatcccgg tgtacactga cgattcgcga
     3721 gcagccaccg gcatgtctga cgtatttgca atcaggaccg tcctcttcat gaggggctcg
     3781 ccactctgtg gatcgagcag ctccggaaat tccatcaaaa cgtctgtcat ctcgttaccg
     3841 cgctcaccac acccaacata aaccactatc tccgcatcag cccacttggc gagctggtgc
     3901 tggacaactg ttttccccga tccgaacggc ccgggaatac acgccgtgcc gcccttggcg
     3961 acagggaaga aggtgtcaag aaccctctgt ccactcacaa gaggttcgct cggtgtcagt
     4021 ttgtgtgcga tcgggcggcc atgtctcaca ggccatctct gcatcatttg gatctcgcga
     4081 actccatcgg acgtttcgat cttggcgatt acgtcagtta ctttggcaac gcccttgtga
     4141 atccatatta ccttgcccga aattccggga ggaaccataa ccctgtgctc cacaacaggc
     4201 gtctcctgca ctgtgccgag aatgtccccg ggaaccacct ttgagccaac gcctgctctc
     4261 ggaacaaagt cccattctct ctcccgatcg agtcccggag cttccagtcc gcgggatatg
     4321 cgatctcccg ccatatccct gatacggtcg agcggtctct gaatgccgtc gtatatggac
     4381 cctatcagtc ccgggcccag ttccactgag agaggttctc cggtaatata gactggttcc
     4441 ccgggcccaa gcccagcggt ctcttcatac acctgtatcg aagcgcggtc ctctctcatc
     4501 tcaatgatct cgcctatcag tcgctgctcg gagacgcgca cgacatcgta catcttggcc
     4561 cccgccatcc ctgaagcgac aaccagaggc cccgatactt ttacaatagt tcctacgctc
     4621 gttccacggt tcatatcagg cattcccctc tctcccgaac aagatgtcgg cgccgagcgc
     4681 tttctccacc gtccgtctta tcttttccat ggccgctccc tgtccccctc gggtatcagg
     4741 tatggtgagc actacaggca gcggcgcctg gctgtactct gtaattacgt cgatgatctc
     4801 gctcgcgtgg gtttcagtaa tatatataac agcataatca cggcgcgccg ccgcgcggag
     4861 cgcctcctct gcttccttgc cattcttcgc ggggaagacc gatacaccaa gagccctgaa
     4921 gcccaagatg gagtcttcgt ccccgataac cgcaatctta tgcatacgca ccactcagcc
     4981 tctcacggat cacattcggc gggatcttgt tcatcttgcc aacaaaaatc cggcgcagct
     5041 tatcagcctc gagctctctt gcgccaaggt aggcaagggc agtctcgtaa cccactacag
     5101 cttctccctc aggcgctatc ttcccaaaaa gatagccttc ggccaggttc tccagcgcga
     5161 caagagatcc attggcaagc caagcgttca tggcatcctc gaccagccgg gcatagggcg
     5221 tatccctgaa ataggccaca aactcgctat caggcttttc atatactccg agcaacgccc
     5281 cggcgtccaa ggtcccgtgg gggactaaaa cgtcactggc gaaacaggcg tccttgccta
     5341 tggcgcgcag cctcatacag atctgaatgt tagtgagatc cgccatggtc gccacgatct
     5401 cctccaagcg cacagcccgt ctagagacag ctacatggtg gaagtagtcg taagatgcgc
     5461 ggtcaatgac gaaatcaatg tactgaggat ctctgccatg ccgcatgtat gcttctaccg
     5521 ccttgtggcc agcatcaatt atcgcatcct caagcggcgt cgtcgctaag atctcatctg
     5581 acccgtcgaa agccacgaaa tcgtcgcgct cgcccggatc atcttcctct tcctcatcaa
     5641 caggataata ctcgagcatt atggcatcag catattctcc gagccgatca gggtcgatcc
     5701 atccgacttg cgacaaccca agcccctccc ggacgatcat gtcccgatcc cgtttgacca
     5761 gccgagactt gagatggact ttcatgttgt gaaaatcgtg tttagctctg aataccccag
     5821 tcaactctgg atcaggcgaa agctcggcaa taagctggtg cacgcgcaca agctccgcat
     5881 cgatgatctc ctcataacgt tcaggaacga tctcacggcc ctgtccatac tcagtttcgc
     5941 tgagaattcg aacaagctcc tcagggctgc ctgcgtctat caggcggttg aacttggctc
     6001 tgtccaccat ccggtcctca agagcccaga tccttcccga tacgtatgca aacctgtcat
     6061 cgaaacccaa agctacgacc tcccgaatag agcctctgca acaaggggcg caagctcgtc
     6121 ttcgcacaac gccaacaacg aatctaagga acaatccacc tcaacggtgg atgctcgaag
     6181 aatgaacccg cctgtgagat ccgctgtttc cgtcgaaagt ttaagtttgg tctgctttcc
     6241 tgccgccacc aactcggaag ctgcttcttt cacgaagtca gagccgatcc gcccacggtc
     6301 aggaagcgca acaaccagtt ctacctggcc tgccggggcc gcattgatca acatcctctt
     6361 catgatcccc aaatactctt catccggcaa cgcacgtatc gtagcaggaa tctccgccat
     6421 gagcttgtca acggcatcgc ccttggcacg aagcatagca ttgcgcacat cgagagaacg
     6481 catgatctcg gcgcgtctct cctcctcgcg cgcatttgcg cgggctcttt cgacagcacg
     6541 cttccttatt tcatccgatt ttgcttcggc ttcccgttga atctcagcgc acctttcatc
     6601 agcggcgtcc agaatcctat ctgctttttc gcgtgcttca tccagaattc ggcccttcag
     6661 tctttcgagg ttttccataa tatacactcc cggctgatca gacgttgatc gacagtacca
     6721 tcaataggct cgcgaggagc gctaaaactg cgtacgtctc caccatggca gctaggatta
     6781 ccgcttttcc catttcctcc ggtctctttg caacaatgct aacgccggac gcggcgacct
     6841 gcccttggaa tatagcggaa agccaaccca gagccgctac ggggacagca gcaaggatga
     6901 acatgatccc ctgattaaca gtgagcgcct ggattgatcc acccaatact cctatcttga
     6961 ccatgatcga caggcccgta aggaaaccgt agactccctg agtgccggga agggcctgga
     7021 ggatcaggat cgaaccgaac ttgtcaggat cttcggtaat gactcctgcg ccggctcgtc
     7081 ccaccattcc gacggccttg gaagatgcgt aaccgccagg aattacggcg agtccaatcg
     7141 ccatgaaccc gagaagtgtg cctagtgtga aatccatgtg tgctatgcct ccttagagtt
     7201 ttccaccgtt acgtactttg tcgacatgcc aaacggcatg aatcttcgcc ctccagcctc
     7261 aaagaactcg ccgaagaact ccacgtactg gaggcggctg gagtggacgt aagagccaat
     7321 gatattgatg aggatattga agaggtggcc gaatgccaac cccactaccg caatgaccgg
     7381 accaactacg ggaatacccg cgggcctcag aaagagatcg ttcatgacag aggccaagac
     7441 agtgcttgta aagcccaagc caaatagcct ggcgtatgat atgacattgc tcatgaaccc
     7501 agtcgtgttg tagagcgata tcagccctgc acctattctc ttaatgggat tcttgtgctg
     7561 gcggccctga gtggcaatga gccctatggc tgaggcaatc agaaaccatt ttgacacagc
     7621 cgctatcgca gggccgttcg ggccgagggc tgtagcgcca agggtcagca tcaggctcgt
     7681 cagccaaaca agccagaatc cctggtcata tatggcggat agccattcgc ccttcttcac
     7741 attgtcaata cccttgatga taatccccca atacagatgg atgatgccca tcacgaaaga
     7801 gagaatgaga aatgtggtcg ggctctccat cggatcaaag aggattgcgg tatcccaacc
     7861 aaaaagacgc ttcggcagat cacccagcca accgccgagc agcgcgccta cgaatatggt
     7921 ggcaaatccg cacatggcaa tgaggtcaag aaacccgcct tcaccaggtc gggtatgggt
     7981 cttcttcatg agttggaagg cgaccaaggc aataaagatg ccatagacgg catctgtcat
     8041 gcacagtccg aagaaaacaa gaaagaatgg gcccaagtat gggctggggt cgatagtgcc
     8101 ctgcttgggg tatcccatgg tcttggttat cacttcaaaa ggagccacaa gcggcttatt
     8161 ggaaagctct accggagcct cttcatcctc aaaccgcggc ctgtccaata ccacgatatc
     8221 atcggcgatc ccttcaatct cggctcgcaa ctcctcgagg tccttagtct gaacccaacc
     8281 ttctaccata aatgtatgtt cagtgccgcc aaacctgttt gtaatggcgt atttctcacg
     8341 ttcaaggtaa aaatggtcgt gaagcacgaa tacgcgggga cggtcctcca cgagggcatc
     8401 gatctcctgt tcgatctgat cgcgtcttcg ataaatgcta tctaattcct ttcgcagacg
     8461 tgcaatgata tccctcggga caccagaata ctgatccagc cttacctcgg caaaacccgc
     8521 ctgcgacaac gcgcgagccg cctcttcagc ctcggaaagg gggtatgccg tcagtaagta
     8581 agctcgctca ccgtcgacgc ccactagctc ccaggcgagt ccttccacaa tctcggaggc
     8641 ttcagcgagg gatgcctcga gtttccgcgc atcggcagtc cccagcacga gctctacatg
     8701 gcgtccggtg cggatctcct cgatcggcgc atccagaccc tgccacggca taagtatctc
     8761 aatcgatgtc aggatggaag cttcattagt tgccagttga gcgagggtgt cttcaatagc
     8821 tttggcccgg ctgtaaatat cgcgagccgc ttccgccccg ccttcagcat aagcattcca
     8881 ttcgtcctca gtcatgtaca gacggaaacc tgaaaagttc tgaacaatgt cccgttttat
     8941 gtgtcgatgc ttacccatca tgtcgatggt gtatgagagc tccgccagaa tgctatcgag
     9001 ctcggcgatt ctttcagaag cgtcgtaggg gcatgcaaga tccaagagtt cttcgtcagc
     9061 aaggtcctca ccaaactggc tcacctctgc agtgcccagt tgttgcagga cgtctacgac
     9121 atctccgatc cgcgactcat gccctagcac ggtcactctg ctcatctcag agatcgccaa
     9181 tagaactcaa caccttccca gcggccaact tcagagcctt gtcccagttt gatctcgcta
     9241 taccgtctaa ttccttcacc ttggactcaa cttgcttctt cgccttgtca gcctcggccg
     9301 ccgtacgagc gcgcgattcg tcctctaccc tggctgcctc agccatggcc gaacgttcgg
     9361 cctctacaac agactctctg gctctctcct ccgctgcggc cactcgagcc ctagcttcct
     9421 caatcgcagc gcgcacgatc tcgtctgcct ggctttcaac atcacggatc tcatctatcg
     9481 cctcgatgct caatgccaac aacaccccca catcgagcag aaaggaccca tgcactacac
     9541 attgttgcat ggactcatcc gggattatac tacaaataca tggtctggtg caacgataga
     9601 agaagtcac
//
LOCUS       DAOSYL010000076         9496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_6161_length_9496_cov_6.189281, whole genome shotgun sequence.
ACCESSION   DAOSYL010000076 DAOSYL010000000
VERSION     DAOSYL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_9496_cov_6.189281"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1627)
                     /locus_tag="PLU15_09620"
     CDS             complement(174..>1627)
                     /locus_tag="PLU15_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO31210.1"
                     /translation="PRTNSLVIVAAQSRMDRALEVVGKLDIEVGTAEEEHDLAQAERP
                     EVVAYRLAYASAESLRPALGLVASPSDLEVDERTNTLIVRAVPSVQERVAELVASLDI
                     PVEVPKAPEPGLEILRIFRLKHASPSEMKSLLGLIAPKAKMQPDDRTGSLVVLAPAET
                     IPELEALIESLDLPSVASKDEPDPVVTRIYRLNYASPSDVERALGSFVEGTIGSDART
                     SSVVVRAAQSEQAKALELIDSLDSELPQVLIEARLEELTGDAAMKLGIDWTFSGLSFG
                     ENAFGQWVSVSMDFLANLTALEESGHATLISRQHTFTVDGKTGKILIGDRIPIITQEV
                     QDGQAVNRVEFINAGIELAITPKVSSDGTITATVKPVISSIVGWTPQNYPQIRTRELE
                     TVVSLKSGQTAVIGGLLHRDEVEGMAKVPLLGDIPLLGELFKKRTTTTNTTELVMLIT
                     AYQVNPGQRSAVGPAQEGDLFPIIVEDALGGKK"
     assembly_gap    1628..1727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1728..4157)
                     /locus_tag="PLU15_09625"
     CDS             complement(<1728..4157)
                     /locus_tag="PLU15_09625"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO31211.1"
                     /translation="MRNRGLGRRAAAALALILCLAFAGQAAFGALERRVDLDLVDAEL
                     ADVFRALAELGDMNIVLDPAVRGTLTIRLHGLAVEDALRLVAYTTGVKYRVEGSTMIV
                     LPPGAVSTSLEPLGIEKFSLTYARTTDVIGPARLVAGGAKLEADERTNSIIACGSAEE
                     LRAIGDVLRMLDVPVQLPPEAIASLADAGLSSGRESLEIIRLQHAPAQGVADLLALVV
                     PADRMRVDSRTNTIVVIIDEASRERAMKIVSEVDVAEPPDDGGFSSVPGKGVNLQTEA
                     VEAETVKVIKLAWAPVDRVREALSVIIDLSKVGMDSRTNSLIVNATPEQIARIEEIVT
                     LLDVAVVAAEKQPAPAHDGIHMFKLEHASAKSVRDVLTMILPPEKMQVDERTNTVVIM
                     ADDDTCARVVELVETLDVETAADQALIGEEKIVETIPLAHASLDAIRDALALVVPKEA
                     VVIDERTRSVIVKGTRSQRERAREIAALLDVPDGPLHETGAATDPSSEPTTNIRAFRL
                     READPESVKEALSMVMPKTSIYVDARTKSVLVVGLLDELARAAEIVALIDQPSRDDVS
                     VGLAKGESEILEMRVIRLVHAPAAKIRDMIAPIMPEIRIAADERSNSLLIVASPSLQE
                     RAASVVTALDIEVPQPEQGTEPAAPRDPEVTRVVTLAHAEAAEVREALAPIVPISSIT
                     VDPRTNSLVIVAAQSRMDRALDVVAKLDVETAASSESLPEEAAHVSESPKAEVVRLKY
                     ASPSKVRDALAPTIPVSRISVDERTGSLVIVGTEAEMTQALAIIDKLDIEVLQPEQGT
                     EPAAPRDPEVTR"
     gene            complement(4154..4768)
                     /locus_tag="PLU15_09630"
     CDS             complement(4154..4768)
                     /locus_tag="PLU15_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31212.1"
                     /translation="MALDLSRESRNEWPSPDGETEAKKRKYRWTALIVALLLVASFSG
                     YVWFLKNRKPDRYPGGSEVVTGGRVAARLSALLDQSHAYGWSRVEEELSPVGHGRWDP
                     FKTLLPDPVQIVPVEPRTPIPALPEPEDSAPCEPALPKVRLTGVLKSGDRAIALLSVD
                     GRSVMTRVGDAPFDGAEVRSIDDKSITISFGGTDFTYQLGGERR"
     gene            complement(4752..5372)
                     /gene="pilO"
                     /locus_tag="PLU15_09635"
     CDS             complement(4752..5372)
                     /gene="pilO"
                     /locus_tag="PLU15_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOO31213.1"
                     /translation="MSLSTEVNPKQAKTFIVLIILAAAGLLYMYVFAPQARKLADART
                     RLDEANSVLTVDLAKIRGAGRSEEELELAMAQLENFERLIPREDRIHYVLRDMESVAL
                     SNHVAIDSLTIASGMPRGQYVEIPMTLNVSGTYNNIIGFLDGIADIERVMNVHSLSFA
                     GGRELWREGGETQDRAVRSPDAYISSTIQVTTYARPRGDDQVGAGS"
     gene            complement(5378..5923)
                     /locus_tag="PLU15_09640"
     CDS             complement(5378..5923)
                     /locus_tag="PLU15_09640"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOO31214.1"
                     /translation="MSKINLLPQREPEESGISPAKLGLIIVLEIAIVGASALYAYRHF
                     FIQQLEEDIDRIEAQIGAIQADVEAVEKVQRRTADIRKRIDEAEAISAEGLASGRVMR
                     EVRDIIPRDIWLSSLSINPDGGVSLAGDTFSIESVARFALAIEDSPMFGEAHVGSVSL
                     AAEGDYEVYDFNIDCMLDARR"
     gene            complement(5923..7050)
                     /gene="pilM"
                     /locus_tag="PLU15_09645"
     CDS             complement(5923..7050)
                     /gene="pilM"
                     /locus_tag="PLU15_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOO31215.1"
                     /translation="MPLRLFSRKSRSMVGVDIGTSRVKVVELVGRGDSVELTRAAIEL
                     APPGAIRDGQIVSPELVAEVIQRGFAKGRIKPGEVVAAIAGRGVVVRHVKFPRMPEEE
                     LREAIKWEGQQHIPMPIEEAVVDFEIIDDYSGSDEAEMEVMLVAAPRKLVESHVQAIE
                     LAGLTPIAIDVQPFAVLRALRYEPGDKTLHPNDRSATAYVDIGAGTTDILIAQGERLR
                     FTRIVPLGGNTFTNAIAETLGVSFEEAEAIKIAKGRVCVDGCGEPCADREEQDISDAI
                     ANVAADVAMDIRRSLDYHDLQLEGRSQDAGVTRVVLTGGASVMEGLDCYLESELGLAT
                     VMGDPLKSILVNQRLANKEELSRLGPMFAVGIGLALREVVD"
     gene            complement(7115..7858)
                     /locus_tag="PLU15_09650"
     CDS             complement(7115..7858)
                     /locus_tag="PLU15_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOO31216.1"
                     /translation="MVSLFSFILGLVIGSFLNVCICRLPSGGSIVNPGSSCPYCGKRL
                     APRDLVPVASYLVLGGKCRYCGEPISIQYPVVEVATGLLYAAIVWANGLTFESLSLLV
                     FVSLVIICAGTDLNTGLILNSVTFPGVVIGVALSFIVPSMTPLQSLLGLLVSGGVVWI
                     MYVVTRGRGMGGGDVKFMAMVGAFLGWDMGLLTLFLGALIGSVVGIARIVSGKQERRD
                     PIPFGPFLSIGAVISVFFGAKLLGFLHLW"
     gene            complement(8096..8443)
                     /locus_tag="PLU15_09655"
     CDS             complement(8096..8443)
                     /locus_tag="PLU15_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO31217.1"
                     /translation="MKRDQRGFTLMELMIVIVIIGVLAAIGVPAYNNYVKKAKAATCQ
                     ANRRTLDTAVGLYYAEHGDYPTADGTEGTSTLQSVLKEYLDNADELKCPEDGQYSLDG
                     ASHKVVCSLAGHE"
     gene            complement(8650..9132)
                     /locus_tag="PLU15_09660"
     CDS             complement(8650..9132)
                     /locus_tag="PLU15_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO31218.1"
                     /translation="MRPIATTVGMLRSIASCILAKEYEVIGMRQDARGFTLVELMTVI
                     VIIGVLSAIGIPSYKGTVDKAKTEACKANRRILSTAVATYYLEHSTYYKLDSAGNPRD
                     EVIEVADLTDYVENAEELKCPAGGNYTIIPGNDGKASSVVIRCDHEGHGDFVVFGTSD
                     "
     gene            complement(9140..>9496)
                     /locus_tag="PLU15_09665"
     CDS             complement(9140..>9496)
                     /locus_tag="PLU15_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO31219.1"
                     /translation="MLQSLDILERLSDNAVVRETVEASKISVRDGAGLARPLRQSQIF
                     PPMLAHMVAVGEETGNLDGMLNKTADFFDQEVEYEVQRVTTAIEPIMTLIIGLFVALI
                     VASVMIPMFNMASFGM"
BASE COUNT         2082 a         2890 c         2341 g         2083 t
ORIGIN      
        1 acaacattgt ggctcaaaca ataattggag gcaatagcgc acgctatttc accagcaagt
       61 gtcgagagcg atcgcccgag aaagcgaccg ctctccacgg catcgcaacg ccaccggcca
      121 tcctggctag atgcggcgct tcacgcgagc ggcccgcaca gcagtcgatc tattcacttc
      181 tttccaccca gcgcatcttc cactattatc gggaataagt cgccctcctg cgcaggtccc
      241 actgccgacc gctgcccggg gttgacctgg tacgccgtga tcaacatcac cagctccgtg
      301 gtgtttgtag tcgtagtccg cttcttgaac aactctccca gaagcgggat atctcccaaa
      361 agcggcacct ttgccatccc ctccacctcg tcacgatgta ggagtccgcc gatgacagca
      421 gtctgtccac tcttaaggga aactacagtt tccagctctc tagtcctgat ctgcgggtag
      481 ttctgaggag tccaccccac tatcgagctg atcaccggct tgaccgtggc agttatagtc
      541 ccgtcgctgc tcactttggg agttatcgca agctcgatgc ctgcgtttat gaactccact
      601 ctattcacgg cctgcccgtc ctggacttcc tgagttatga tagggatcct gtcgcctatg
      661 aggatcttcc ctgtcttgcc gtcaacggtg aaagtatgct gcctgcttat tagcgttgca
      721 tgtcccgatt cctccaaggc ggtaaggttg gcaaggaagt ccatgctaac actcacccac
      781 tggccaaagg cattctcgcc gaatgagagg cctgaaaagg tccagtcgat acccagtttc
      841 atggctgcgt ctcccgttag ttcctccagc ctcgcctcga tcagcacttg gggaagttct
      901 gaatcgaggg agtctatgag ctcgagtgcc ttcgcctgtt cggactgggc cgctcttacc
      961 acgactgagg atgttcgggc atctgatcct atggttcctt ctacgaagct tcccagggcc
     1021 cgctcgacgt cagaaggcga tgcgtagttg agcctgtata tcctggtcac caccgggtca
     1081 ggctcgtcct ttgacgccac tgatggaagg tccaggctct cgatgagcgc ctccaattca
     1141 gggatggttt cagcgggagc gagaaccacg agagaacctg tccggtcatc cggctgcatc
     1201 ttggcctttg gcgcaatcaa gcccaaaagc gatttcatct ccgacggcga ggcatgcttc
     1261 agtctgaaaa tccgcaatat ctcgagcccg ggctcggggg ctttcggcac ctccacaggt
     1321 atgtcgaggg acgccacgag ctctgctaca cgttcctgca ccgaaggcac ggccctgacg
     1381 atcaaagtat ttgtcctttc gtcaacttcc aagtcggaag gggacgcaac gagacccaaa
     1441 gcagggcgca gcgactctgc gctcgcgtat gcaagcctgt aggccacgac ctcaggccgc
     1501 tccgcctgcg ccaagtcatg ttcctcctcg gcagtgccaa cttctatgtc gagcttcccc
     1561 acgacttcaa gggccctgtc catcctcgac tgcgccgcca cgatcaccaa ggaattcgta
     1621 cggggatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ggtcacctcg
     1741 ggatcgcgcg gcgctgcagg ctcggtgccc tgctcgggct ggagaacctc tatgtcgagc
     1801 ttgtctataa tcgcaagggc ctgtgtcatc tcggcctctg ttcccacgat aacgagcgac
     1861 ccggttcgct cgtccacgct tatcctcgat acgggaatcg taggcgccag ggcatcccga
     1921 accttcgatg gcgatgcata tttcaacctc accacctcgg ctttcgggct ttcggacacg
     1981 tgcgccgcct cctcagggag gctctcggaa ctcgccgcag tctccacatc cagtttcgcc
     2041 acaacgtcca gcgccctgtc catcctcgac tgcgccgcca cgatcaccaa ggaattcgta
     2101 cggggatcca cagtgatgct ggagatcggc actatcgggg ccaaagcctc acgaacctct
     2161 gcggcttcgg catgggcaag cgtaaccacc cgggtcacct cgggatcgcg cggcgctgca
     2221 ggctcggtgc cctgctcggg ctggggcacc tctatgtcga gagccgtcac cacgctagct
     2281 gcgcgctcct gcaaagacgg ggaagcaact atcagcaatg aattgctacg ctcgtccgcg
     2341 gcaatcctga tctccggcat tatcggagca atcatatccc gaattttcgc agccggggca
     2401 tgaaccagcc ggatcaccct catttccagg atttccgact cgccttttgc caaacccaca
     2461 ctgacgtcat cacgagatgg ttggtcgata agggcaacga tctccgctgc acgggccagt
     2521 tcgtctaaga gcccgacgac caatacagat ttggtgcgcg cgtccacata aatagaagtc
     2581 ttcggcatca ccatcgagag ggcctcttta acggattcag ggtcggcctc acgaagcctg
     2641 aatgcgcgga tgttggttgt cggctccgaa gagggatctg tcgcggctcc tgtctcatgt
     2701 aaaggcccgt ccggcacgtc aagaagggct gcgatctcgc gggcacgctc acgctgcgac
     2761 cgggtgcctt tcacgattac cgaccgcgtc ctctcatcga ttacgacagc ttctttcggc
     2821 accaccaaag ccagggcatc tcgaattgca tccaaagagg catgggccaa ggggatagtc
     2881 tcaacgatct tctcttcccc gatgagtgcc tggtcggccg cagtttctac atccaaagtc
     2941 tcaacaagtt ctacaactcg agcgcaagta tcatcatccg ccattatcac cacggtgttc
     3001 gtcctctcgt ccacctgcat tttttcaggg ggcaggatca tggtaaggac gtctcgcact
     3061 gatttggctg atgcgtgctc gagcttgaac atatgtatgc catcatgggc tggtgcaggc
     3121 tgcttttccg cggctaccac agccacatcg agaagagtca caatctcttc aatccgggct
     3181 atctgttcag gcgtcgcatt tacaatgagc gaatttgtgc gtgaatccat tccaaccttg
     3241 gaaaggtcga tgatgacact gagggcttca cggaccctgt ccacgggggc ccacgcaagc
     3301 ttgatcactt tcaccgtctc ggcctcgact gcctcagtct gcaggtttac acccttcccg
     3361 ggaacactcg aaaaacctcc atcatcaggg ggctctgcca cgtccacttc ggatactatc
     3421 ttcatcgctc tttcacggga ggcctcgtca atgatgacca ctatggtgtt cgtgcgtgaa
     3481 tccacccgca tcctgtcggc aggaaccacc aaggccaaca ggtcggccac cccctgggcg
     3541 ggggcatgtt ggagcctaat aatctcgaga gactcgcggc ccgaagacaa cccagcatcg
     3601 gcaagcgaag cgatggcctc cggaggcaac tggacaggaa cgtcaagcat cctcagtaca
     3661 tcacctatgg ccctgagctc ttcggctgac ccacaggcga tgatggagtt ggtccgctca
     3721 tccgcctcca gtttcgctcc gcctgcaaca agccttgcag ggccaataac gtcagtcgtt
     3781 ctggcatagg tgagcgagaa cttctcgatg cccagcggct ccaggctggt tgaaacagcg
     3841 cccggcggaa ggacgatcat tgtagagcct tcgaccctgt atttcacgcc ggtagtgtag
     3901 gcgacgagcc taagcgcgtc ttcgactgca aggccatgaa ggcgaattgt gagggttccg
     3961 cgtaccgcgg ggtcgagcac tatattcata tctcccagtt ccgctagggc gcgaaacacg
     4021 tctgcaagtt cagcatctac caggtcaaga tcgacgcggc gctccaaggc gccaaatgca
     4081 gcctgcccag cgaaggccag gcacaagatg agagccaatg ctgccgccgc tcgccttccc
     4141 aatcccctat ttctcatctc cgctcacctc ccagttggta agtgaaatca gtcccgccaa
     4201 acgaaatcgt tatggacttg tcgtcgatag agcggacctc tgcgccatcg aagggcgcgt
     4261 ctccgacgcg cgtcatcacg cttctcccgt ccactgaaag caatgcaatc gccctatcgc
     4321 ccgactttaa gactccggtc agccgcacct tgggcaaagc cggttcacaa ggcgctgaat
     4381 cttcgggctc aggcaaagct ggaatcggcg tcctcggttc cacggggact atctgcaccg
     4441 gatcgggaag caaagtcttg aacgggtccc aacgaccatg ccccaccgga gaaagctcct
     4501 cttccactct ggaccaacca tacgcgtggc tctggtcgag cagagccgag agcctggcag
     4561 caacacgccc tccggtaacc acttcagacc caccaggata ccgatcgggc tttcgattct
     4621 tcaggaacca cacgtagccg gaaaatgacg cgacgagcaa cagcgcaact atgagggcgg
     4681 tccatctgta tttcctcttt ttagcttcgg tttcgccgtc tggagacggc cactcgttcc
     4741 gggattctct gctaagatcc agcgccaacc tgatcatcac ccctcggcct ggcgtatgtc
     4801 gttacctgaa ttgttgaaga tatataagca tctggcgaac ggactgctcg atcctgggtt
     4861 tcaccgcctt cccgccagag ttctcgccct cctgcaaaag aaaggctgtg cacattcatg
     4921 acccgctcaa tgtctgcaat tccgtccaga aagccgatta tgttgttgta tgttccggaa
     4981 acgttcaatg tcattggtat ctcgacgtac tgcccccgcg gcatgccgga ggcgatggtg
     5041 aggctgtcaa tggcaacatg gttggacagg gcgactgact ccatatcgcg gaggacgtag
     5101 tgtattctgt cttctcgggg aataaggcgc tcaaagttct ccagttgggc catggcaagt
     5161 tcaagttctt cttcggacct cccggcgcct cgaatcttgg ccaagtctac tgtcagcacg
     5221 gagttggcct cgtcaagacg cgttctggcg tccgccagtt ttcgcgcttg gggggcgaac
     5281 acgtacatat aaaggaggcc cgccgcagcc agtataatca gcacgataaa tgtcttggcc
     5341 tgcttggggt tgacttccgt cgaaagagac atgcctgtca cctccgagca tcaagcatgc
     5401 agtcgatgtt gaagtcgtac acctcgtaat ctccctcagc tgctaagctt acagacccca
     5461 catgagcctc gccgaacatg ggcgaatcct cgatagccag tgcaaagcgg gccactgatt
     5521 ctatggagaa ggtgtctcct gcaaggctca ctccgccatc ggggttgatt gaaagagacg
     5581 aaagccagat gtccctggga atgatgtccc gaacttctct cataacacgg cccgatgcga
     5641 ggccctcagc gctgatagct tcagcttcat ctattcgttt cctgatatcg gcagttcgcc
     5701 gttgcacctt ttccacggcc tcgacgtctg cctgaatcgc tccaatttgg gcctctatcc
     5761 tatcgatatc ctcctccaat tgctgtatga agaagtgcct gtaggcatag agcgctgaag
     5821 cgcccactat ggcaatctca aggacgatta tcagtccgag cttggccggg cttatcccgg
     5881 attcttccgg ttcccgctgc ggaagcaggt tgatctttga catcagtcaa ccacctcccg
     5941 aagggcaagg cctataccca cagcgaacat ggggccaagt ctcgacagtt cctccttgtt
     6001 ggctagcctc tggtttacaa ggatgctctt cagtgggtcg cccatcacag tcgcaagacc
     6061 cagctcgctc tcgaggtagc agtcaaggcc ttccattact gatgcaccac ctgtaagaac
     6121 taccctagtc actccggcat cctggcttct tccctcaagt tgcaggtcgt ggtagtcgag
     6181 ggaacgtctt atatccatag caacgtctgc ggcgacattc gcaattgcat ccgagatgtc
     6241 ctgttcctcc ctgtctgcac aaggttcgcc gcaaccgtcg acacaaaccc ttccctttgc
     6301 aatctttatt gcctctgctt cctcgaagct aactcccaac gtctcagcga tagcgttggt
     6361 gaaggtgttg ccgcctaagg ggacgatcct tgtgaacctg agtctttcgc cttgggcgat
     6421 gagtatatcg gtagtgccgg cgccgatgtc gacatatgcc gttgctgacc tatcgttggg
     6481 atggagcgtc ttatccccag gctcatatct taaagccctg aggacagcaa atggctgaac
     6541 atctatggca atgggtgtaa gtccagccag ctcgatagct tgtacatgcg attccaccag
     6601 cttccgcggg gccgcaacaa gcatcacttc catctcggcc tcatcagaac ccgagtagtc
     6661 gtctatgatc tcgaaatcga ctacggcctc ctcaatgggc atggggatgt gctgttggcc
     6721 ttcccacttg atcgcctctc tcagttcctc ttcgggcatc ctaggaaact tcacgtgcct
     6781 tacgactact ccccgaccag ctattgcagc gactacttcg ccaggcttaa tccgaccctt
     6841 agcaaagccc cgctggataa cctccgccac cagttcaggc gagactatct ggccgtcccg
     6901 gatggccccg ggcggcgcaa gctcgatggc ggcgcgggtc agttcaacac tgtcgccgcg
     6961 gcccacaagt tccacaacct tgacgcggct ggtgccgata tccactccga ccatgcttct
     7021 gctcttccgg gaaaagaggc gcaacggcac tcatacgcac cccctcttga aaagactcgt
     7081 atcaacatac tcttcgactg taatctgaaa actcctacca taagtgaaga aacccgagca
     7141 gctttgcacc gaagaagaca gaaatgacag cccctattga caagaacggt ccgaacggaa
     7201 tgggatcacg cctctcctgc ttgcccgaga caattcgggc tatccccacc accgagccaa
     7261 tcaaggctcc aaggaaaagc gtgagcagcc ccatatccca gcctaagaag gctccgacca
     7321 tagccatgaa cttgacgtct cccccgccca ttccgcgacc ccgggtaacc acgtacatga
     7381 tccaaacaac gcctcctgag accagcaatc ccaacagcga ttgaagagga gtcatgctcg
     7441 ggacgatgaa tgatagagcg acgcctatca caactccggg gaaagtgacg gagttcagta
     7501 tgaggccggt gttgagatca gtcccagcgc agatgatgac cagcgagaca aaaacaagga
     7561 gtgaaaggct ctcaaatgtg agaccgtttg cccatacgat tgcagcgtaa agcaacccag
     7621 tcgccacctc gacgacggga tactgaatcg agatcggctc cccgcagtat ctgcacttcc
     7681 ccccaagcac caggtagctt gccaccggga ccaagtcccg cggcgcaagg cgcttgccgc
     7741 agtatgggca cgaggaaccc gggttcacga tagagccccc ggacggcaga cggcagatgc
     7801 atacattgag gaaactgccg atcacgaggc cgagtatgaa tgagaataga gagaccatgt
     7861 acggcaaaga aaaacatcct ttgcgattct aagtgggagt aaacagtctt atcagaacat
     7921 attcaatcag catctaggat aagagacccg aaccacctgg agcaattcgg gtctcctagt
     7981 tgccatcgcg aattcgcact agactcgtga ctgttagctg gggaacacat cagatcgtgc
     8041 caactaaccc aaacccgatt tggtagcggg gcacacgatg acaaatcacg aattgctatt
     8101 cgtgcccagc gagactgcaa acaaccttat ggcttgcccc atcgagcgaa tattggccgt
     8161 cttccggaca cttcagctca tctgcattat caaggtactc cttcaatacc gattgcaaag
     8221 tgctcgttcc ctctgttcca tctgctgtag ggtaatcacc atgctcagcg tagtaaagcc
     8281 ctacagcagt atcaagagtc ctcctatttg cttgacatgt cgccgcctta gccttcttga
     8341 cataattgtt gtacgcaggc actccgatcg ccgccaacac gccgatgatc acgataacga
     8401 tcatcagctc catcagtgtg aagccgcgtt gatccctctt cattgacata atcctccccc
     8461 tcaattctgt aacactctga caatctttga agccacttaa acccaaggcc tgtccacccg
     8521 aaccgtccct gtgcagcgcc gacgtttggc taaagctata tatgtaaatt cgtctagctg
     8581 ccaacctcgc agtctctcaa ttaccaaagg ccgccgcatc cacaccatgc cagctggcgc
     8641 ccggcaatac taatcactcg ttccgaaaac tacgaaatcc ccatgccctt catggtcgca
     8701 acgtataacc acactcgagg cttttccatc gttaccgggt attatcgtgt aattcccgcc
     8761 tgcagggcac ttcagttcct cagcgttctc cacataatct gtaaggtcag caacttcgat
     8821 tacctcatct cgcggatttc ccgccgagtc gagcttgtaa tatgtcgaat gctccaaata
     8881 gtaggtggcc acggcagtgc tcaagattct cctgttggcc ttgcatgcct ccgtctttgc
     8941 cttgtcgact gtgcctttgt atgagggtat gccaatcgcc gacagtacgc ctattatgac
     9001 gatcacagtc atcagttcaa cgagagtgaa tcctcgcgca tcctgcctca ttccaatcac
     9061 ctcatactct ttggccagaa tgcagctggc gatcgaccgc agcattccaa cagtggtcgc
     9121 aatcggcctc acctgcaccc tacatcccaa agctcgccat gttgaacatc ggaatcatca
     9181 cagatgccac tatgagagcc acaaaaagcc cgattatcaa tgtcataatg ggctctatgg
     9241 ccgtggtaac tcgttggacc tcatattcga cttcttggtc gaagaaatca gcagttttgt
     9301 tcaacatgcc gtcgagatta cccgtttcct cgccaactgc gaccatgtgc gcaagcattg
     9361 gcgggaatat ttgcgattgt ctgagaggtc tggcaagtcc ggcgccatca cgaacagata
     9421 tcttggacgc ttccactgtc tccctgacta cggcattatc tgacaacctt tcaagaatgt
     9481 caagagattg caacat
//
LOCUS       DAOSYL010000077         9483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_6173_length_9483_cov_8.352249, whole genome shotgun sequence.
ACCESSION   DAOSYL010000077 DAOSYL010000000
VERSION     DAOSYL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6173_length_9483_cov_8.352249"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..2295
                     /locus_tag="PLU15_09670"
     CDS             433..2295
                     /locus_tag="PLU15_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO31220.1"
                     /translation="MKLSIRKKLIFSYVIVLALAVGVAAAGIWSNQRTAQRYTDIIKL
                     SLPLETQILTVRAHMLERSVVLRDYILTQDEFCISEYESINESIDKALQAMDDYDLHA
                     QSIEYRDAIMDFESMYRDMANRMFIAISDGSVDQAVNLLIGEETFANAALTDSINSWR
                     DYIVNVNDEWIAEANRIARNGLIIIGICLVIALAAVIALSIILSKNIAVPVERIQAAA
                     DLMSMGDLTVAIPSIETGDEIEALNESVRRMTENLASLVREVQGTAEQVAGSSSQLSA
                     AAGESAQAVDQIAATVQQMANAAEQESSSANETAAASEQIMASVNQVASGAETQMMQV
                     KNTSELVDRMTEELSKVTGFLKELQKDMEITVKSAEEGNASVQEVEYIMNQIRQASAE
                     VESAAKGLDESSRQIGQVVQVISDIADQTNLLALNAAIEAARAGEQGRGFAVVAEEVR
                     KLAEGSLEETKTISKLIERTVADTGRVAKAIVASGELIEKSVPVVSAATASLEVIRKN
                     AQNNLAVAGSTATLGEALMNDTIQVNQGMSQVVAVSDENAAAAQQMAAGMAEVQKSVE
                     NIAAISEENAASIEEVSASSEEVNASIREIHAASELMADMAHKMMNDVAKFKVA"
     gene            complement(2559..2816)
                     /locus_tag="PLU15_09675"
     CDS             complement(2559..2816)
                     /locus_tag="PLU15_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOO31221.1"
                     /translation="MIEVRIYGPLRERLGTGSLEVEASRAGSVGELLAVVSEVTEAAS
                     LSELRKASVFVNGTNIVDLRLFRTALLPGDEVVLLSPSGGG"
     gene            complement(2820..4724)
                     /locus_tag="PLU15_09680"
     CDS             complement(2820..4724)
                     /locus_tag="PLU15_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOO31222.1"
                     /translation="MEGRYRGYAGETLLIDLGSRQVSTYPWTDLERELYLGGKIMAAK
                     IMHDIVDPEVEPFSPENPLVVTTGPLTGSGAPSSSRFNISTLSPLTNLLTSSNCGGNF
                     GFHLKKAGFDGVIITGKSPEPVWIEIDDGKVVFHDAGELWGKTTGETQEALPKNAGKM
                     VIGPAGENLVLYASIVSQERVAGRAGVGAVMGSKNLKAVTARGSRRMVLYDEEKAKEI
                     FKEWVKALRGHPLTGVQLPQLGSAGLVAPMQYNKILATRNFARGRFDEFEAVSGETLR
                     EKHLIRNKGCITCPIQCGRVVEVDGKEVKGPELETLGLLGPNLENSNLELILEWNYLL
                     DELGMDTISAGSTVGFAMELNEKGLWDNGLAFGEVDNLADVFRQIACREGIGDLLADG
                     TKRLSERFGGREFAIHSKGLELSAYEPRRSVGMGLGYATSNRGGCHLNGGYLVLLEGL
                     ALNIDPLTTNSKAALTILFQNIMEAVSAGGSCLFTCYLAMPAFMLEDANAWTTRWINR
                     ALTRHGHLMGAVAKMPGWMMPFHFPGLWHTQALTAVTGMKMTFGRFKEIGERGYNLER
                     MFNAKRGVTAADDALPKRLTDDLQDPEVPGSRVPLDDLKKEYYKLRGWDFNGLPKKEK
                     LRKLGLAGVD"
     gene            complement(4743..5924)
                     /locus_tag="PLU15_09685"
     CDS             complement(4743..5924)
                     /locus_tag="PLU15_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO31223.1"
                     /translation="MIPSYYEFINSVKILSGKNALENIPAELRGFEARKPILLTDKML
                     VKIGLAQQVVDACGGSEIVIGAMYDDIPPDSSVEVVNDIVHVYRETGCDSVIAVGGGS
                     VIDTAKGVCICLTEGTDDLLDHMGVDILRRRKTPFVVVPTTAGTGSEVTGVAVIANPA
                     KNVKMEFVSANILPDVAVLDVRMTKSLPPRLTASTGMDALVHAIEAYTCLQRNPLSDA
                     HAHAAINLIRKYLPRAVRDGKDEEARLAMANASLLAGVAFSNSMVGIVHAVGHACGGV
                     LRAPHGDCMAILLPYGMEYNLSAVSERYAELLLPLAGPEVYARTSASQRAEKSIETVR
                     EMSAEYSRICGLPTKLREVGVKKEDFSAIARAAVNDGAMIVNPVEADEDDVLGILEKA
                     Y"
     gene            complement(5942..6457)
                     /locus_tag="PLU15_09690"
     CDS             complement(5942..6457)
                     /locus_tag="PLU15_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31224.1"
                     /translation="MASAYREIRPPAKRFEKSCVSVLLWFVGRGFQAAAHVDKHVQDE
                     VCGWKDGTIIAIGVAPCGPHMSVRKTGNRLRFLGNVKAPDADAVIEFKSLDAALLVLT
                     GQIGIDRAYAEHRFSLKGDLFSIGMPLVRCLYIVESYLFPTSIAKKILKRWPKRSSSG
                     FAVYFHTIFGV"
     gene            6980..8110
                     /locus_tag="PLU15_09695"
     CDS             6980..8110
                     /locus_tag="PLU15_09695"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO31225.1"
                     /translation="MDLISGLQQMGLTEYEAKIYAALVKQPSITGYEASKLSGVPRAK
                     VYETLESLVRKKAAYSLSVDGKQYYTPISHHSLIARFQDELLDLTTYLSVELDRLSKG
                     RTEDPFSVVTGYDLVTETAERMCARSQRRLYISVFAEDLPHLAGEIAKAKSRGVKVFT
                     LVYGEGKGIVEDAVEHYVSPLQYLQAAVYGRWMGLVADQDEGLMAQIAGPDDTYGLSS
                     TLPGIIMPITLWIQHDIAAYVVAEKLNLPPTVTDGLEDLWSLAPEEGPQAPEHGNKSL
                     DDLFEVAKRKLEHNAGTLKDGIIEICFTDDGITYHVSKGQHKVEIEQGAKEEADLRLA
                     LSKADFEGLMTGFLSPEAFMTRGRIEIDGDLGMVAYLNAMFR"
     gene            8150..8863
                     /locus_tag="PLU15_09700"
     CDS             8150..8863
                     /locus_tag="PLU15_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOO31226.1"
                     /translation="MEIDKSANLPVREMLEGFCTSYIAKTPNPVDISFGFLINDGQWW
                     TVSIQKEGSYTIANCKPSKPTFYLVSTAKTLEDIYLGKMHFLTAAGRAKMNDYAPLDV
                     RFIDGYSPPKDLDLMDFAFHFFVIGEPEKILFGKDHARVVHGGYAVPLVYTSGLRTAW
                     YRVEQGMVINEKEEDQYNPFNTLVIGTKGEGSIKLGHTEYRFAEGEAYIIPANTAHSF
                     WTDDEDGLEFVIIMYGEGA"
BASE COUNT         2435 a         2726 c         2270 g         2052 t
ORIGIN      
        1 accgggcaat tcacacggtt ccggttcaag ctgcttaatg caccaggttg gttagcatgt
       61 caagtgtgag aatgagtgga accattttgt gtcgtcatag tacgtgttgg caggctgtct
      121 aataccggaa agcgcatacc ggcacgcctc tagtcacatt acatattatc gtcctgcgcc
      181 atatgtcctt ctcgaaatcc tggtgttttt cacaacattt gctccttgcg tgatgccgcg
      241 ccccgagccg gaatgcaact cgttgcaggc ataataccaa catctatctt ggtttcccca
      301 cacacttgaa tgtttggcta tatccggtct ataccatcca tgtagtgtcc ttccgacaca
      361 ttttttcctg tgaagatcct gctcaaacct gccgataaag gtattaagcg aagccatgga
      421 ggtgcaatgc ggttgaaatt gagcattcgc aaaaaactga tcttctctta cgtaatagtg
      481 ttagcactgg ccgttggcgt tgcagctgca ggcatctggt caaaccagag aacggctcaa
      541 cgctacacag atatcatcaa gctaagcctc cctcttgaaa cacagatatt gacggtccgt
      601 gcacacatgt tggaaagaag cgtcgtgctc cgagactaca tcctcaccca ggacgaattc
      661 tgcatctcag aatacgagtc catcaatgag agcatcgata aggctctgca ggccatggat
      721 gactatgatc tacacgccca gtccatagaa tatagagacg ccataatgga ttttgaatcc
      781 atgtacaggg acatggcaaa ccgaatgttc atcgcaatca gcgatggctc tgtggaccag
      841 gccgtgaatc tgctcatcgg cgaagaaacc tttgccaacg cggcactaac cgattcgatc
      901 aacagctggc gcgattacat cgtcaacgtg aacgatgaat ggattgcaga agcaaacaga
      961 atagcccgaa acggtctgat catcatcggg atatgcctgg taatcgcatt agcggctgta
     1021 attgccctct ccataatcct ctcgaagaac attgctgtac cagtcgaaag aatacaggca
     1081 gcagcagacc tcatgtcaat gggtgacctt actgtcgcca tcccatcgat cgagacaggg
     1141 gacgaaattg aagccctaaa tgagtctgtg cgtcgaatga cggagaatct cgcaagcctt
     1201 gtccgcgaag ttcagggcac tgcagagcag gttgccggat caagctccca gctgtcagcg
     1261 gccgccgggg aatcagccca agccgtggat cagatagcag caaccgtgca gcaaatggca
     1321 aacgccgccg agcaggaaag ctcttctgca aacgagacag cagccgccag tgaacagata
     1381 atggccagcg tgaatcaggt tgcgtcaggc gccgaaactc aaatgatgca agtgaaaaac
     1441 acatccgaac tggtagatcg catgaccgaa gaactcagca aagtgacggg tttcctcaaa
     1501 gagctgcaaa aggatatgga aattaccgtc aagtctgctg aggaaggtaa cgcatctgtc
     1561 caagaggttg aatacataat gaaccaaatc cgacaagctt cggccgaagt ggaaagcgca
     1621 gcgaaaggcc tagacgaaag ctcacgccag attgggcagg ttgttcaagt aataagcgac
     1681 atcgcagacc aaaccaatct gttggcccta aatgcagcca tagaggcagc gcgcgccggc
     1741 gaacagggtc gaggttttgc ggtagttgct gaagaagtgc gcaaacttgc agaaggatcg
     1801 cttgaagaaa ccaagaccat ctccaagctg atagagagaa cagtagctga tactggacgt
     1861 gttgccaaag ctattgttgc atcaggagag ctgatagaga aaagcgtgcc tgtagtatca
     1921 gcagcaaccg cctctttaga ggtcatacga aagaacgctc agaacaacct tgctgttgca
     1981 ggctcgacag ccacactagg agaagcactg atgaatgaca caatccaagt caatcagggc
     2041 atgagccaag tggttgccgt atccgatgaa aatgccgctg cagcccagca gatggcggca
     2101 ggcatggcag aagtgcaaaa atccgtagag aacatcgctg ccatatccga agaaaacgca
     2161 gcctcaatcg aagaggtatc cgcatcctcc gaggaagtga acgcctcaat acgtgaaatt
     2221 catgccgcgt ccgagctgat ggccgacatg gcgcataaga tgatgaatga cgtcgccaag
     2281 ttcaaggtcg cctagttgcg ctttgaacta ggctacgttg tgttagagag ccctgctcct
     2341 gcagggctct tctattagca atttgcctcc ccaagtgaac cgttcggcca tgctccgatg
     2401 gggacatcgc caatccccaa caccttctgc aactgtaagg ccggctaaaa atcatgccgg
     2461 actcaatatc aagattcacc aaacagacct gagggcatct gaacgtaagg gccttgtcat
     2521 ctctttttga ggtgaacctt gggcaccgct cctgccttct acccgcctcc cgacggcgaa
     2581 agcaatacca cttcatcccc gggcaacaga gcagtcctga acagcctcaa atctacgata
     2641 ttcgtcccat tcacgaaaac tgacgctttg cgcagctccg acagactggc cgcttcagtg
     2701 acttcgctta ccacggcaag cagttcgccg acggagcccg cccgcgatgc ctcaacttcg
     2761 agcgaacccg tacccaatct ctccctgagc gggccataga tgcgcacctc aatcatgaat
     2821 caatccaccc ccgccaggcc aagcttgcgc agcttctctt tcttgggcag cccgttaaaa
     2881 tcccaacctc tcagtttgta gtactccttt ttcaggtcat caagcgggac cctgcttccg
     2941 ggaacctcag gatcctgcag gtcatcggtg aggcgtttcg gcaacgcatc atctgccgcc
     3001 gtcactcccc ttttcgcgtt gaacatgcgt tcgagattat atcctctctc tcctatctcc
     3061 ttaaacctcc cgaaggtcat cttcatcccg gttacagcag taagagcctg cgtatgccag
     3121 aggccgggaa agtgaaacgg catcatccag cccggcatct tcgcaacagc tcccatcaga
     3181 tggccgtggc gagtgagtgc gcgattgatc cacctagttg tccatgcgtt cgcatcctcc
     3241 agcatgaacg cgggcatggc aaggtaacac gtgaacagac agctccctcc cgccgacacc
     3301 gcctccataa tgttctgaaa aagaatggtc aacgccgcct ttgagttcgt cgtcaacggg
     3361 tcgatattga gtgccagccc ttcaaggaga accaggtatc ccccgtttag atggcatcct
     3421 cccctgttcg atgtggcata gcccagcccc atcccgactg aacgccgcgg ttcatacgct
     3481 gacagctcca agcccttgga gtgtatagca aactcccggc cgccgaacct ctcggaaagg
     3541 cgcttggtcc catcggccag aagatcccca atcccttccc tgcaggcgat ctgcctgaac
     3601 acgtctgcaa ggttgtccac ctcgccaaag gcgaggccat tgtcccacaa acccttttca
     3661 ttaagctcca tggcaaatcc aacagtgctg ccggcggata tggtatccat gccgagctcg
     3721 tccaaaagat agttccattc cagaataagc tcgagattgg agttctccag gttcggcccg
     3781 agcagcccca aagtttcgag ctcagggcct tttacctcct tgccgtccac ctccaccacc
     3841 cttccgcatt ggatggggca cgtaatgcat cccttgttcc ttataagatg cttttcccgc
     3901 agcgtctctc cggatacggc ctcaaactcg tcaaatcggc cgcgagcaaa gtttctcgtc
     3961 gcaagtatct tgttgtactg catcggggcc acgagccccg ccgaccccag ttgaggaagt
     4021 tgcacccctg tcaatggatg ccccctgagt gccttgaccc actctttgaa tatctccttt
     4081 gccttctcct catcgtaaag aaccatcctc ctgcttccgc gagcagtaac agccttcaag
     4141 ttcttcgacc ccattaccgc cccaaccccc gcccgcccgg caactcgctc ctggctcaca
     4201 atagacgcat aaagcacgag gttttcgcca gccgggccta tcaccatctt ccctgcgttc
     4261 ttcggaagag cctcctgagt ttcccctgtc gtctttcccc aaagctctcc ggcgtcgtgg
     4321 aaaacgacct tgccatcgtc tatctctatc cagacaggtt ccggagattt accagtaatt
     4381 attacgccgt cgaacccagc cttcttcagg tggaacccga aattcccgcc gcagttggac
     4441 gatgtcaaaa ggtttgtgag aggcgacaat gtggagatgt tgaacctgct ggagctgggc
     4501 gcccctgacc cagtgagagg gcctgtggtc acaaccagcg ggttttcagg ggaaaacggc
     4561 tccacttcgg gatccactat gtcatgcatt atcttagccg ccatgatctt gccgcccaga
     4621 tacagctccc gttccaggtc ggtccatgga tatgttgaca cttggcgcga acccaagtct
     4681 atcaacaaag tctctcctgc atagccccta tatcgaccct ccatagcctc tccagcctcc
     4741 catcaatagg ctttctccaa gatgcccagc acgtcgtctt catccgcttc aaccggatta
     4801 actatcatag cgccatcatt cacagccgcc ctggctattg ccgaaaaatc ctcctttttc
     4861 acgccaacct ccctcagctt agtcggaagc ccacagatcc tgctgtattc tgcactcatc
     4921 tcccgaacag tctcaatcga cttttcagcc ctctgcgacg cagaagttcg cgcatatact
     4981 tccggtccag ccaatggcag aaggagttcc gcatagcgct ccgacactgc actcagatta
     5041 tattccatcc cgtaaggaag caatatcgcc atgcagtctc cgtgtggcgc tcgcaacacc
     5101 cctccgcatg catggcccac ggcatggact atgcccacca tggagttgga gaaagcgacg
     5161 cctgccagaa gcgatgcatt ggccatggca agccgggctt cctcgtcctt gccgtctcga
     5221 actgctcggg gcagatactt ccggatcagg ttgatggccg catgagcatg tgcatcgctc
     5281 aagggattcc tctgcagaca cgtataggcc tcaatagcat gcacaagagc atccatgcca
     5341 gtggacgcag tgagacgtgg cgggagcgac tttgtcatcc tcacatcgag gacagcaaca
     5401 tcaggcagaa tgttggccga tacaaattcc atcttcacat tctttgcagg gtttgcgatc
     5461 acagcgaccc cggtaacctc ggaacctgtc cccgcagtgg taggcactac cacgaaaggc
     5521 gtcttacgtc tcctcagaat atcgacaccc atgtggtcga gaaggtcatc agtcccctcg
     5581 gttaagcaaa tacatacgcc tttagcggta tctatcaccg aacctccccc aacagcaatg
     5641 accgaatcgc atcccgtttc gcgataaaca tggactatgt cattgaccac ttccactgag
     5701 gaatcagggg ggatatcatc atacatggcg ccgatcacaa tttcagatcc gccgcacgca
     5761 tctacgacct gctgcgccag gcctatcttg accagcatct tgtcggtgag caggattggc
     5821 tttcgggcct cgaatcccct gagttctgcc ggaatattct caagagcatt cttgcctgat
     5881 agtatcttca cagagtttat gaattcataa taagaaggaa tcattctcca gcctccttct
     5941 attacacccc gaaaatggtg tggaaataga ctgcaaaccc agaggaactc ctctttggcc
     6001 atctcttcag aatcttcttg gcaatgcttg tggggaagag gtacgactcc acaatgtaga
     6061 ggcacctaac gagcggcatg cctatggaaa acagatcccc tttcaaagag aatctatgtt
     6121 ccgcgtaagc ccggtcgatt ccgatctgtc cggtaaggac gagcagagcg gcatcgaggc
     6181 tcttgaattc gatgacagcg tccgcgtccg gagctttcac attgccgagg aaacgcaacc
     6241 tgttgccagt ttttctgacg ctcatgtgcg ggccgcaagg cgcaactcct atagcgataa
     6301 tagtcccatc tttccatccg cacacctcgt cctgcacatg tttgtctaca tgggcggccg
     6361 cctgaaaccc tctgcccaca aaccacaaca gaaccgaaac acagcttttc tcaaacctct
     6421 ttgccggtgg ccttatctcc cgataagcgc tggccaaatc gctgacccct ctctttcctt
     6481 tgtgtatact cgctctcgcc cataccgcgc atcgtgcccg ctgcatagct ggatataggc
     6541 ggacaacgcg ggaaagaaca ccccgtttta gcgatttatc tgcacaccaa agtgacagtt
     6601 gcccgtctga tcactatcgt gctcggcaac accctaccac acaatcagca ggccactaat
     6661 gagcatcctc attatcggaa ctagcggatg ctctcagctc cagtccgagt tgcctatata
     6721 tagcttgtct tccttgaaaa gacgcctcaa tcctgtcacg ccacctcccc catccgtatt
     6781 tcccccagct gattagaatg tatcacggca catatacatg tcaactaagt actactctac
     6841 ctcaaatccc ttcaatttac atatcggctc ctaggcataa aacgcaagtt ctagtgcttg
     6901 acatctgctt ggcgctgata gataatgaaa tggcagttta gtaactactt tagtagttac
     6961 tatccgctgg gaggttgcca tggatctgat aagcggtctt cagcaaatgg gcctgactga
     7021 atacgaagcc aagatatacg ctgccctcgt caaacagcca agcattacag gatacgaggc
     7081 cagcaaattg tcgggggtgc cacgagccaa agtatacgaa acccttgagt cattggtgcg
     7141 aaaaaaagcc gcatattcac tatcagtcga cgggaagcaa tactacactc caatctctca
     7201 ccacagcctt atagcccgat ttcaggatga gctattggat ctcaccacct atctctccgt
     7261 cgaattggac aggcttagca aagggcggac agaagaccct ttttcggtcg tcacaggata
     7321 cgatctagtg acggaaaccg cagagagaat gtgcgcgaga tcacagagaa ggctatacat
     7381 atcggtattt gccgaagacc ttccccacct ggctggcgaa atcgcaaaag cgaaatcgag
     7441 gggcgtcaaa gttttcaccc tcgtttacgg ggaaggcaaa ggtatagtag aggacgccgt
     7501 agagcactac gtcagccccc tccaatacct gcaagcagcg gtgtatggca gatggatggg
     7561 ccttgtagca gaccaggacg agggcctcat ggcgcagatt gccggaccag acgataccta
     7621 cggactctcc tcaaccctgc cggggatcat catgccaata accttgtgga tccagcatga
     7681 catcgcggca tacgtagtgg ccgaaaagct caacctccct ccgaccgtca ccgacggact
     7741 ggaggatctg tggtcgctcg cccctgaaga aggccctcaa gcaccagagc acggcaacaa
     7801 gtcactcgat gatctgttcg aagtggcaaa acgcaaattg gagcacaacg caggcacact
     7861 gaaggacgga atcatcgaaa tatgtttcac cgatgatggc ataacctatc atgtttcaaa
     7921 aggccaacac aaagtggaga tagaacaagg cgcaaaggaa gaggcagacc tcaggcttgc
     7981 attgagcaaa gccgattttg aaggcctgat gacaggcttt ctctctcccg aagcattcat
     8041 gacgcggggg cgcatcgaga tcgacggcga cctcggcatg gtggcctatc tgaatgcgat
     8101 gttcagatga gaaaaaagag cttaaactca tgcaaaagga gcgatccaca tggaaattga
     8161 caagagcgcc aatctgccag ttcgggagat gttggagggt ttctgcacgt cctacatcgc
     8221 caaaacccca aacccggttg acatctcctt cggtttcctc atcaatgacg gccaatggtg
     8281 gacggtctct atacagaagg aaggcagcta cacgattgcg aactgcaagc cttccaagcc
     8341 cacattctac cttgtgagca cagctaaaac actggaagac atctacttgg ggaagatgca
     8401 tttcctaacc gccgcaggaa gagccaagat gaacgactac gccccgctcg atgtcaggtt
     8461 catcgatggc tatagcccgc caaaagactt ggacctaatg gatttcgcat ttcacttctt
     8521 cgtaatcgga gaaccggaga agatactatt tggcaaggac cacgccaggg tggtgcatgg
     8581 cggatacgca gtgcctttgg tatatacttc aggcctcaga acagcctggt acagagtgga
     8641 acagggcatg gtcatcaacg aaaaagagga agaccagtac aatcccttca acactcttgt
     8701 catcggcaca aaaggagagg gctccataaa gcttggacac acagaataca ggttcgcaga
     8761 aggagaagca tacataatac cggcgaacac cgcccacagc ttctggacag atgacgaaga
     8821 tggacttgag tttgtgataa tcatgtacgg cgaaggcgct tagacaccag cccgagatgc
     8881 ctgctcgaac atatatcagt tggatgggga tggacgagag tttccggagc tcacctgata
     8941 aatgacccgc agagggtcaa tttaggcgaa gtacgactct ctggcattca tttcgttcgc
     9001 agaatgaccg ccagtgggcc actcaccacc ccaacacgga aagcccgggt cagccagcta
     9061 cgtacagtac gcccgggagg aggaatgatt aacatggagt cagtttcggg ggtaatgacc
     9121 gggtgcgtgt tactccccct aagaaacgac caccatctgc tcacccctcc ccgccacaag
     9181 cccccgcctg ctgcaacatg ccgtcacgcg caaaacgcaa tgggccatgg acaatcacca
     9241 cctctgctct cctaatgccg taacatacta acattcctct acaaaagcaa gaacgctaat
     9301 ataaacatag tggagtgcaa ccttttcgtc cccgcccatc tcttaatagg tggagggggg
     9361 agaagagctg tggccatagg gagctatcta tgcctatgcg tctcgatgca cgaacctcat
     9421 gtgaagaggc ttaaggcagg ccatgaggaa gcggcgaacg agttggtcga catgtacacc
     9481 gac
//
LOCUS       DAOSYL010000078         8485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7127_length_8485_cov_6.566074, whole genome shotgun sequence.
ACCESSION   DAOSYL010000078 DAOSYL010000000
VERSION     DAOSYL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7127_length_8485_cov_6.566074"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            576..1955
                     /locus_tag="PLU15_09705"
     CDS             576..1955
                     /locus_tag="PLU15_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO31227.1"
                     /translation="MIHADEACLILNPTLGTWVKVSRYAGVMCERFLGVDIQGITDSV
                     GPAYGKETLDGLLGLMRDLEARGLLVYGPWNGGGKSDVAAAEKRAAQVCAAPARIYVS
                     VTDRCNLYCETCYRHAFENDAPTESVLWTIREAGMFRPAELMITGGEPTLRADLPVLL
                     EEATFNAPKVILATNATLITQEMAEAIASLGVCVQVSIDSGDEAAHDMIRGEGSFKAA
                     IRGIERLVRAGTEHVELVSTIVDPATFNPDDMIELASKHGATFHASLFQEVGRGACSR
                     LKGRRKCPKDLAEAMLRYLLRACHRNALPEDSSFEDVVGLVPREGCGAGSKVFALSGA
                     GNAYPCHLLMIPEFAATLYDISEPPRRPTLKRVEEAHACGRGDLKELFWDVPGVDQIE
                     GCLECDVRYLCGGGCRAAAYAASGSLYGRDPMCEAYRIFISSILWTWDDSRTTSSNLL
                     RALSEVAML"
     gene            1952..2719
                     /locus_tag="PLU15_09710"
     CDS             1952..2719
                     /locus_tag="PLU15_09710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO31228.1"
                     /translation="MSSVICYIIVGISVAVRAYAQKSHERGQPGVDLGDILLLTIFAG
                     VWIVRREDPANSLRIVEGMASWEMAVYTGLIVVVAGLANLWAHARLRQSHGEWMKGAG
                     INPGGAYLALSGMPKWTALVLAVPFIASGALLEEIVFRGFLLRTISKRSSLSNGLVVQ
                     AVLFGLVHGIPMALVEAPNSITSYALLMPITVGLALGWLTVESGGLAYPWIAHWSLNY
                     AAFAVEMSRLSRERRLEAVASNVDVDTANVDTDTFWG"
     gene            3132..4430
                     /gene="obgE"
                     /locus_tag="PLU15_09715"
     CDS             3132..4430
                     /gene="obgE"
                     /locus_tag="PLU15_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOO31229.1"
                     /translation="MFVDLVEICVRGGDGGHGEVAFRREKYVPRGGPSGGDGGNGGSV
                     IAEADKALTTLTDFRYRKHLRAKNGARGGGSKMTGAAGEDLIIKVPVGTIVRDAETGE
                     MVADLVVHGQRAFLARGGAGGRGNARFATSVRRTPRFAEKGELGEERRLVLELRLLAD
                     VGLIGYPNAGKSTLLSKISSARPKIADYPFTTLSPILGVVDLADKSFVVADIPGLIEG
                     AAEGIGLGHEFLRHIMRTRVLVHVVDVSEESGRDPLEDYRTIRRELELYHPDLPVKPE
                     IVAANKVDMPGASDRARAFSDVLEKEDVQVFPISALTGEGVQSLLYAVADVLAKIPPD
                     DILVEPEEEARVYTQRGSRLRDTVVERDGDVYVLSGEGVERLCQMTDMDNVEAYGRFW
                     AMLAKAGIVDELRKAGAVEGDIVRIGDLEIEYGEAHFWTR"
     gene            4453..5055
                     /gene="nadD"
                     /locus_tag="PLU15_09720"
     CDS             4453..5055
                     /gene="nadD"
                     /locus_tag="PLU15_09720"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOO31230.1"
                     /translation="MKRIGLMGGTFDPVHYGHLVTAEQAYYQFDLDKVVFVPSGQPPH
                     KMGKQITSREHRYMMTVLATTSNPHFEVSSIEVDRSGPSYAIDTVRAFRDRYGYDAGL
                     YFITGADAILEMLEWKDSDDLLDLCEFIAATRPGYALHSDKIEQIFGHRANKIHFLEV
                     PALAISSTDIRERLKDGRPVRYLLPDMVINYIELVGLYRE"
     gene            5133..5390
                     /locus_tag="PLU15_09725"
     CDS             5133..5390
                     /locus_tag="PLU15_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO31231.1"
                     /translation="MSKTLYVGNLPWGITDHQLQQVFSPYAEVISSRIVTDRDTGRSR
                     GFGFVEVDTDDIEPIVKSLHGTTIDGREITVNEAKAREHRM"
     gene            5490..6122
                     /gene="yqeK"
                     /locus_tag="PLU15_09730"
     CDS             5490..6122
                     /gene="yqeK"
                     /locus_tag="PLU15_09730"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOO31232.1"
                     /translation="MCEDALTSIRDELESTLQPERFAHSLRVEEESRRLAEKYGADVR
                     VCGLAGLLHDCARDLPDDALLTLAHRSGRCDLVESLQNCPMLLHGLAGAVLAREKYGV
                     EDVRVLRAIACHTLGSIEMSAEDKVVCLADYIEPGRKFPGVDRLRALAEHDLDLALLS
                     ALDCTILHLVQTGREIAIETVLARNRLVRAVNTNCEAGDPPAREFGEIAG"
     gene            6082..6489
                     /gene="rsfS"
                     /locus_tag="PLU15_09735"
     CDS             6082..6489
                     /gene="rsfS"
                     /locus_tag="PLU15_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOO31233.1"
                     /translation="MIHLLVNLEKLQAELSSTCHAKKAAAAAVEKKAVDVVLIDMTSV
                     SIMADYFLVCTVYTEAHARGVREAIEESMEESKLALRRREGSDASGWVLLDWGDIVVH
                     IFREEERKYYDLEGLWGDAPAVRVGDSSADDAT"
     gene            6850..6925
                     /locus_tag="PLU15_09740"
     tRNA            6850..6925
                     /locus_tag="PLU15_09740"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6883..6885,aa:Ala,seq:ggc)
     gene            complement(7050..7697)
                     /locus_tag="PLU15_09745"
     CDS             complement(7050..7697)
                     /locus_tag="PLU15_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO31234.1"
                     /translation="MRIAQPRMEPGPWLLSPSPRHQHIAGGVFFAVKLPPARKPKKEA
                     FTELEIRQIEKAAEAGDIWAGTIMILLYTGMRVGELVTLTPFQVDLDARIITGGIKTD
                     AGRDRPMPVHSRILSYVQYWYNTGGPRLIHKDGKPINVNYYRKYCFYPALERAGVTRH
                     LTPHATRHTFATLLARAGVQPAHIQALMGHSDYAVTANVYTHLAMDELRRAVESI"
     gene            complement(7711..>7995)
                     /locus_tag="PLU15_09750"
     CDS             complement(7711..>7995)
                     /locus_tag="PLU15_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31235.1"
                     /translation="ALDGTVASEVLNTYLPGYGVVFLFSVEYGMALDDVQYQVERALR
                     FITPTIGVLPEGEVIAIVGYSSRDDWEIMYVSQADSSADPNTWDVYFNKR"
     assembly_gap    7996..8005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1865 a         1993 c         2635 g         1982 t
ORIGIN      
        1 cgataaatac cgtcaaaact cacattcgtt cgatatatca aaaactggga gtgcacaccc
       61 gggggtcggc agtcaggctg gcggttgctg ccggattgtt ggacagttgg tcacctcctc
      121 ctagcccgta aaatcatcct ttcgggcaat acccttaagc tccctgcgat ggtactatgt
      181 gggtgcaagc ggcgccgtgg cgtggtcgcc atctacccga ttgtagcatc gagggaggtg
      241 aaatggatgt ttcgcattca ggcaggtcgc aagcataggt tgaatgtctc tgtcaacagc
      301 ttctgctttt cgtttccgct ttggggcgtg atgctcacca ttggatgatc ttcgtttccc
      361 cagactacta gtcaccatca tccattagcg ggcgtagctc caactgtgcc agcgggggcc
      421 aatcacaaga gattggttcc cgcgcctatg tgcgcgcgct gtggctttga agggaggagt
      481 agggcggcga tgtggcccga tgttggcggg gcgcaaccgc cttttcgctg atgaactccg
      541 aattgttggg atactctcta gcgccggatg cacggatgat tcatgcagat gaagcttgtc
      601 tcattctcaa tccgacgctc ggaacttggg tgaaggtcag ccgttatgca ggcgtgatgt
      661 gcgagaggtt cctcggagtc gatattcaag gaataactga ttctgtaggg ccggcttacg
      721 gaaaggagac gctagatggc ttattggggt tgatgcgcga cttggaagcc cgaggattgc
      781 tcgtctacgg gccctggaat ggaggcggaa aatcggacgt agcggcggca gaaaaaaggg
      841 ccgctcaggt ttgtgccgcg cctgctagga tatatgtttc cgttacggac aggtgcaatc
      901 tatactgtga gacatgctac agacatgctt tcgaaaacga cgccccaact gagtcagtgc
      961 tttggacaat acgagaggca gggatgtttc gtccggcgga gctgatgatc actgggggtg
     1021 aacctactct cagggcagat cttcctgtat tgctcgagga ggcaacattc aatgctccca
     1081 aggtgatttt agctaccaat gctacactga taacgcagga aatggctgaa gcaatagcct
     1141 ctctgggggt ctgtgtccag gtgagcatag attcagggga tgaggctgca cacgatatga
     1201 tcaggggcga aggaagcttc aaggcggcga ttaggggcat cgagaggctc gtgcgtgccg
     1261 gtacagagca cgttgagctt gtgtcaacca ttgttgaccc agcgacattc aatccagacg
     1321 acatgattga gcttgcatcg aagcatggag cgacattcca tgccagcctt tttcaggagg
     1381 ttggccgagg agcatgttca cggctaaaag gacgcaggaa gtgtccgaag gatcttgcgg
     1441 aggcgatgtt gcgctatctt ctgagggcgt gccacaggaa tgctctgccg gaggattcgt
     1501 cgttcgaaga tgtagtgggg ctagtcccga gagaagggtg tggggcgggc tcgaaggttt
     1561 ttgcactttc gggggcaggc aatgcatacc catgccatct tttgatgatt ccagagtttg
     1621 cggctactct ttacgacatt tctgagccgc ctcggcggcc tacactgaaa agagttgagg
     1681 aggctcacgc ttgcggccgt ggggatctga aggagctttt ctgggacgtt cctggggttg
     1741 atcaaataga aggttgcctc gaatgcgatg ttcgctactt gtgcggaggg ggctgcaggg
     1801 ctgccgcgta tgcagcgagc ggaagtctgt acgggcgtga tccgatgtgt gaagcatata
     1861 gaatctttat atccagcatt ctatggacgt gggatgattc ccgtacgacg tcgtctaatc
     1921 tgctgcgagc tctgtcagag gttgccatgc tatgagctca gtcatttgtt acatcatcgt
     1981 aggcatctcg gtagctgtaa gggcatatgc gcaaaaatcc catgaacgtg gacaaccagg
     2041 agtcgatctg ggagacatcc tgcttctgac tatcttcgca ggggtctgga tcgtgcgaag
     2101 agaggatccg gccaactctt tacgaatagt ggagggcatg gcaagttggg aaatggcggt
     2161 ttacaccggg ctgattgttg tggtggcagg cttggcaaat ctgtgggcac atgctaggct
     2221 ccggcaatcg cacggcgaat ggatgaaggg tgctggcatc aacccgggag gggcgtacct
     2281 tgctctctcc ggcatgccaa aatggactgc tctcgttctt gcggttccct ttatagcctc
     2341 gggcgctctt cttgaagaga tcgtgttcag gggctttctg ctccgcacca tatcgaaacg
     2401 gagcagcctt tcaaatggcc tagttgtgca ggcggttttg ttcggactgg ttcatgggat
     2461 tccaatggct ttagtggaag ctcctaacag cattacctcg tacgcactcc tcatgcccat
     2521 cactgtgggc cttgctttag ggtggctcac ggtggaaagt ggcggcttgg catacccgtg
     2581 gatagcgcat tggtccctga attacgcagc ttttgcggtc gaaatgagca ggctgtcccg
     2641 ggagcgaagg cttgaggccg ttgcttctaa cgtagatgtg gatacggcaa acgttgacac
     2701 tgatactttt tggggctaac aacgtcgctg acagcggaga caacgttgct ggtatgcgtg
     2761 tcccccgcgc gggttggggt ggattggcag tcggacgcat gtttgcgcgg tgcgcgcaga
     2821 ctggcaaacc agggtcaggc atctgccata gatgcgctgc attgaaggaa gactatgcag
     2881 cgatgtagaa ttagaagacg cgcgttgcag gcgatcagtg taatcgggca gacgtcgtcc
     2941 gacctcctca aactcttcga tggtcggttg atcgtaggcg agcgcgtatt ggagcccgcg
     3001 aagtaacgta tgggcctgga tcgtgtttgg tcaggcgagg ccagcctgga ggttgattgt
     3061 aggggctgtg gcttttcggt tcacagttcc tccgaatatg taggggcgtg cgggcaagga
     3121 gagcagttga catgtttgtt gatttagtcg aaatatgcgt tcgtggcgga gatggtggcc
     3181 acggcgaggt cgcattcagg cgggaaaagt atgttccgcg gggcggccct tcagggggag
     3241 acggtggcaa tggcggaagc gtcatcgcgg aggcggacaa ggctttgacg acgctgacgg
     3301 actttcgtta ccggaagcat ctccgcgcaa agaacggcgc ccgtggtggc ggcagcaaga
     3361 tgactggcgc ggcaggcgaa gatctcatca ttaaagtccc agttggaact attgtgaggg
     3421 acgcggagac aggtgagatg gtggctgatc ttgttgtcca tgggcagagg gcattcttgg
     3481 ccagaggcgg ggcagggggg cgtggaaatg ctcggttcgc gacatcggtg cggaggacgc
     3541 cgcgttttgc tgagaaggga gagcttggcg aggaacgccg actcgtgctt gagcttcggt
     3601 tgttggcaga tgtggggctc attggatacc ccaacgcggg gaaatccacg ttgctgtcaa
     3661 agatttcttc tgctcggcca aagattgcgg attatccatt cacaaccctc agcccaatcc
     3721 tgggcgttgt ggatctagcg gacaaaagct ttgttgtggc ggacattcct gggcttatcg
     3781 agggtgcggc ggagggtata ggcttgggac acgagttctt gcgccacatt atgaggacgc
     3841 gggttcttgt tcacgtcgtt gatgtatcag aagagtcagg gcgcgatcct cttgaggatt
     3901 atcgaactat caggcgcgaa cttgagctct accatcctga tttgccggtg aaaccagaga
     3961 ttgttgcagc gaataaggtg gacatgccgg gggcttctga tcgggctagg gctttttcgg
     4021 atgtgctcga aaaagaagac gtccaggtgt ttcccatatc ggcgttgact ggcgaaggcg
     4081 tccagagcct gctgtacgca gttgctgatg tattggccaa gatcccgcct gatgacattc
     4141 tggttgagcc tgaagaggaa gcaagggtgt acacccaacg tggttctagg ctgagggata
     4201 ccgtggttga acgggatggc gatgtctatg tgctgtcagg cgagggcgta gaacgtctat
     4261 gccagatgac tgacatggat aacgttgaag cttacgggcg attctgggct atgttggcca
     4321 aggcgggaat cgtagatgag cttaggaagg ccggagctgt tgaaggagat atcgtgcgca
     4381 taggagatct ggagatagag tacggcgagg cgcatttctg gacgcgctag caggtgcggc
     4441 aggtgtatta cgatgaagcg tataggcctg atgggcggca cttttgatcc ggtgcattat
     4501 gggcaccttg taacggcgga acaggcgtac taccagtttg acctcgacaa ggttgtgttt
     4561 gtgccatcag ggcagcctcc ccataaaatg ggcaaacaaa tcacttcgcg tgaacacaga
     4621 tacatgatga ctgttctggc cacaacgagc aatccccatt ttgaggtgtc ttcaatagaa
     4681 gtggacaggt caggtccatc gtatgccatc gatactgtga gagcttttcg agatagatat
     4741 gggtatgatg caggacttta tttcatcaca ggcgcagacg caatactgga aatgcttgag
     4801 tggaaagatt cggacgatct tcttgaccta tgtgagttta ttgctgcgac gaggccgggg
     4861 tatgcgcttc acagcgataa aatcgagcaa atattcgggc acagagcaaa caagattcat
     4921 ttccttgaag tgccggcctt ggcgatatca tccacggata ttcgagagcg gctcaaagat
     4981 ggacgaccgg ttcgatatct tctgcctgac atggtcatca actacattga actggtcggg
     5041 ctatacagag aatgactgcg cgctgcatct tgggtgatac ataagtgcac gggtacggcg
     5101 gaaaactacg cttaacggag tgaggtgcag gcatgagtaa aactttgtat gtgggaaacc
     5161 tcccttgggg gataacagat catcaactac agcaagtgtt ctccccatat gcggaggtca
     5221 tttcaagcag gattgtgacg gacagagata ctgggagatc ccgcggtttt ggttttgtgg
     5281 aggtcgacac cgatgatatc gagccgatcg tcaaatcact tcacggaacg actatcgatg
     5341 gaagagagat cacggtgaac gaggcgaaag cccgggaaca ccgcatgtaa caaaggatgt
     5401 tgaagtgcat cgcccccacg tggaacctct gggggcgatg tgctattatg tgatgtagtg
     5461 aactgtcaat gctggggggt agcttcttga tgtgcgaaga cgccttgaca tcgatcaggg
     5521 atgaactcga atcgacgctg cagcctgaac ggtttgccca ttcgctgcgg gtggaggagg
     5581 aatcgaggcg gcttgccgag aagtacgggg cggatgttcg agtctgtggt ttggcagggc
     5641 tcctccacga ctgcgcacgg gatctacctg acgacgcact cctaacactt gctcatcgaa
     5701 gtggccgttg tgatctggta gagagcctgc agaactgccc gatgctcctt cacggacttg
     5761 caggcgcggt tttggccaga gaaaagtatg gagtggaaga tgtgcgggtg cttcgggcaa
     5821 tagcatgcca tacattggga tcaatagaga tgagtgctga agacaaggta gtgtgccttg
     5881 ccgattacat tgaacctggg agaaagttcc cgggtgtaga taggctccgc gcccttgccg
     5941 aacacgatct tgatttggct cttctatctg ccttggattg caccattttg catctggtgc
     6001 aaactgggcg tgaaatagcc atagagactg ttttggcgcg aaaccgcctt gttcgcgcgg
     6061 tgaatactaa ttgtgaggct ggtgatccac ctgctcgtga atttggagaa attgcaggct
     6121 gaactctcat ccacatgcca cgcaaagaag gcggctgcgg ccgctgtgga gaaaaaggca
     6181 gtagacgtgg tcttgatcga tatgacttcc gtttcaatca tggccgatta cttcctggtg
     6241 tgtacggtgt atactgaggc gcatgcgcgt ggcgtgcgcg aagcaatcga ggagagcatg
     6301 gaagagtcga agttggcgct tcgccgccgc gaaggctcag atgcatcagg ctgggtgctc
     6361 ttggattggg gtgacatagt agtccacatc ttccgggaag aagaaaggaa atactacgac
     6421 ctagaaggcc tctggggcga tgcgccagct gtgagagttg gcgactcaag tgcggacgat
     6481 gcaacttaac gtcagattcc tggctgagcg gcgtgatgtt ggaccgatgg gcgtctgtgc
     6541 ggcaatgggc tgcacggcta atcgtcaaag tggtcgttgt cggaagaatg tcagcgggtt
     6601 atgaggcgaa ttggcttgac ttaccgaaac ggggcaggta taatcatagc tgcttgatat
     6661 tttgaaatgc aaacgcaatg aagaggagca ataagtgctg tgccgctcaa agagagccgg
     6721 gggtgggtgg aacccggtgt gcgggcagct ctgaactcgc cttggagcgg tgcaggtgaa
     6781 gtggaaagag attgtagtct gcaacgggtg gcagcgttat atgcccaaaa gacacgctca
     6841 agtttgcgtg gggctgtagc tcagctggga gagcgcttga atggcattca agaggtcgag
     6901 ggttcgatcc ccttcagctc caccattaag aatacgaaag ggcctgctaa agcaggccct
     6961 ttcgcttttt gacgcgaccg aaaatgtgtc tgaaaacggc cggaaatcgg ggtcgtaata
     7021 cacgcacaat acgcatacaa tacacaaacc tagatcgact cgacggctcg gcggagttcg
     7081 tccatggcga ggtgggtgta aacgttggcg gtgaccgcgt agtccgagtg tcccatgagc
     7141 gcctgaatgt gcgccggttg cacgcctgcc cgggccagaa gcgtggcgaa cgtgtgccgg
     7201 gtggcatggg gcgtcaggtg acgggtaaca ccggcccgct ccagcgcggg gtagaagcag
     7261 tacttgcgat agtagttgac gtttatcggt ttgccgtctt tatggatgag ccgcggcccg
     7321 cccgtattgt accagtattg gacgtaggac aggatgcgac tatgcaccgg catgggacgg
     7381 tcccggccgg cgtccgtttt gatgccgccg gtgataattc ttgcgtccaa gtccacctga
     7441 aacggcgtta aagtcacaag ctcgcccacg cgcatgcccg tgtacagcag tatcatgatg
     7501 gtgccggccc agatatcacc ggcttcggca gccttctcga tctggcggat ttcgagctca
     7561 gtaaatgctt ccttctttgg tttccttgcc gggggcaact ttacggcgaa aaacacgcca
     7621 ccggctatgt gctggtggcg tggtgatggg gagagtaacc aaggccccgg ttccatccgg
     7681 ggctgggcaa tacgcattac ctaatcagca ttacctcttg ttgaagtaaa cgtcccaagt
     7741 gttcgggtcc gcactgctgt ctgcttgtga cacatacatg atttcccaat cgtcccggga
     7801 cgagtatccg actatggcga taacctcacc ctcgggcagc acccctatcg taggcgtgat
     7861 gaaccgcagg gcacgctcca cttgatactg gacgtcatcc agagccatcc cgtactccac
     7921 cgaaaacaga aataccacac cgtacccggg gagataagtg ttcaggactt cgctggctac
     7981 cgtaccatcg agcgcnnnnn nnnnntccat atcctggcgt agttccaccc agccggggat
     8041 cgtcttgggt tctgcagcta cggacgttat ggtgcctagg agcatcatgg cgatagcgac
     8101 ggacactaca aagcgcttca taccgacacc tccgcatagt ttgatattca cttccccttt
     8161 gttcgacatc ttttggtcaa ttcctttcta cttccacttt gattttgaac actgagaggg
     8221 cacccgcgag ggtgcttttc ttcgcctgcg gccttcggcc ggggcttaat ttttccgaga
     8281 ctttgcgtca gatcccttga ctctactata ccgcgtgcgg tataattctg tagcgagggc
     8341 cgcgggcgac gtgggacgcg gagaaatgcc acccgtaata cacacgcaat acacacagcg
     8401 ggattcaaac ctcggtatta ccgcattcga cgcttgaatg gcattcaaga ggtcgagggt
     8461 tcgatcccct tcagctccac cattt
//
LOCUS       DAOSYL010000079         8407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7210_length_8407_cov_8.657807, whole genome shotgun sequence.
ACCESSION   DAOSYL010000079 DAOSYL010000000
VERSION     DAOSYL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7210_length_8407_cov_8.657807"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLU15_09755"
     CDS             complement(<1..616)
                     /locus_tag="PLU15_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31236.1"
                     /translation="MTRSKLWRAIMFVLMVLAVSGLAVVAQGGQPPGGGPGTPPEPPG
                     DGFRPGMPGPGGTEIAPPPVGSTYITGTGVYTLSSGSAAMSSETLTASADDQSAIIVS
                     DGAQLNLSGMTIKTSGNTSSMDSSSFYGLNAAILAQAGSRVSITDSTIITSGTGANAV
                     FACGAGASIDMANVDIACEAPGAHGVDATMEGTITMRDVNITTAG"
     gene            1162..2208
                     /locus_tag="PLU15_09760"
     CDS             1162..2208
                     /locus_tag="PLU15_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31237.1"
                     /translation="MSQPEYASPSLEVWKDLYEAAAQFKRIESWNWLDDSSTFGVQDP
                     VTKTIAYCSVMGALGELYGLAAYVGTEGLLALQKMAFGETDYSDINLGKDMRCLMTTF
                     ESRNDLDKPDIEVIRQLGLSFRGKHNWPRFRSYEPGFFPWYLDAHEAMFVTTILQQAV
                     DVCLRAKDDPDLIPNWDDEVYLVRVAEEKDGELVWEDRMLSPELLPEPELEDSVHIDE
                     LQLMKIRNSAKTTDAVWDADLFYAPACVQEDPKSRPYYPLMSLWVDHGTGLIIGTKMA
                     GHDDYRQAFVDELINMVNQDNVKPREIRVKREDAYRLYEGVASKLGIPMRKVQSLGML
                     EGIQADLAEFLMRR"
     assembly_gap    2272..2371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2476..3657)
                     /locus_tag="PLU15_09765"
     CDS             complement(2476..3657)
                     /locus_tag="PLU15_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOO31238.1"
                     /translation="MKKRSGIIALLIMTLLALECTAVHAQEYADYPPDGMEPQFLKAR
                     VLSVEKVISDYAVPDDFAVESEVIKLELTSAPYKGVVVQSTSLKSGHPAYDFDVEPND
                     RVIVWAEILDGNIVEAHVFSPDRGHYLKWLVVGFMGVLLLVGGLTGIKTILTLGVTGL
                     AIVWILLPVLLRGYDPIYVTILVSSAITVITLGIVGGFNKKTLTAMIGTTVGVIIAGL
                     IAMIVGSAAHLTGFGTEEATMLFYIPQEVQFDFDRLLFSGMIIGALGAVMDVAMSIAS
                     SMEEVMLARPRITQEQLLRSGLRVGRDVMGTMSNTLILAYAGGSIPLLLLFLAYETPI
                     VQIVNFDHIATEIVKALAGSIGLIAAIPITALVGSRLFKTARPGSAKKPASLQRQKEV
                     I"
     gene            complement(3835..4503)
                     /locus_tag="PLU15_09770"
     CDS             complement(3835..4503)
                     /locus_tag="PLU15_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOO31239.1"
                     /translation="MPEIVIAVAGQPNSGKSTIFNMLTGLRQFAANYPGVTVEKRVGR
                     LDYSGHKFTLVDLPGTYSMTSYSLEERLARDFLLNEKPDVILNVVDASNLERNLVLTF
                     QLLEMRMPMVVALNMMDAAENAGIEIDVERLSKKLGVRAVPTVAKKGIGKPELCDALL
                     KAYECRDEANDFRLDYGPQLNRSLEVLEEALAGDSRASGYDPRWLAVRLIAGDNEISR
                     IFAD"
     gene            complement(4491..4736)
                     /locus_tag="PLU15_09775"
     CDS             complement(4491..4736)
                     /locus_tag="PLU15_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOO31240.1"
                     /translation="MNDAVRLDSLKPGDTCRLHDIKANDATAQRFMDLGLIPGVEIKV
                     VRNAPLVDPVELLVKNNYVSIRHSEARRLEVIPLCRR"
     gene            complement(4743..4985)
                     /locus_tag="PLU15_09780"
     CDS             complement(4743..4985)
                     /locus_tag="PLU15_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOO31241.1"
                     /translation="MDKTIMPLTELPRGCKGTVVNVGNYGRGLARRMSDMGLYDGSEV
                     MVISSAGAGPVLMQVNDCTIAVGRGVAKKILVAKEM"
     gene            complement(5008..6825)
                     /locus_tag="PLU15_09785"
     CDS             complement(5008..6825)
                     /locus_tag="PLU15_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOO31242.1"
                     /translation="MNLLQLVAEERERLLREEQSEPARLVTTAYYRQASRVFDECAVR
                     EAKQKESLSNRIDRIVLHRFFGPLILAGILYVLYYLSIAKGYQLTNYTWPLLAGFRNW
                     VAQILPPEGILFDPTVRDMVLSVVDGLLAVINYVPIFAILFSLVAILEDVGYVPRMAF
                     IMDRILRQFGLHGQSVFPMVLGGVVVGGCAVPAIMATRGMKDQRARLATILIIPLLNC
                     LAKTPLHILLIEMFYGEHKGFAVFFISTISLIIALTVSKLLSLTVLKVKESAPFVMEM
                     PAYRLPTIRGVFQRCWERLWMFFRKIVSVVLLVMIVVFALTNLPGLNASKKTAYSAEA
                     DKMVDAFLASVDDDSVYAQLLSGNGIVELIDFSNQFDAGKKSARSEAKLDKVHKDFMA
                     KNLEFYKLVNRGRFETNGMIEVDPEAARISRAYRSFERERKALRARASDDIIVHSALG
                     IVGRALEPVTKYAGFDWKVNIALISSFAAKESSVATLGSIYGLDTGVRLEESVAEGDL
                     GWTDLHALALMVFMVVFPPCIPTLLAVKMETGSTMWMLLAAFYPVLLGLLLASLIFTG
                     GNALGLSGLQAMIRFYAISIMLMLVLATVKGPSEDEFDQ"
     gene            complement(6893..7696)
                     /locus_tag="PLU15_09790"
     CDS             complement(6893..7696)
                     /locus_tag="PLU15_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOO31243.1"
                     /translation="MARPHKPWNRVVYTLTMALAIIAVYSASSMAHFQMVLPSDDIIE
                     ENESNVVQLQLIFTHPAEASHTMNMDKPIEFGVYHKGKARDLTDTLEAFEFHGAKAWQ
                     TEFTARGAGDFVFYLRPSPYYESMEDSYITQYTKVVVNNAGFPTDWDVEIGLEAEIVP
                     LCKPYGLWTGNVFQGVVKQNGKAVPFAEIEVERLNADAFSSFAGLGQTYPSSAHVTQI
                     IRADGNGVFTYGIPKSGWWGFAALMEGESIDGKDHEIGAVIWVKAYDME"
     gene            complement(7722..8228)
                     /locus_tag="PLU15_09795"
     CDS             complement(7722..8228)
                     /locus_tag="PLU15_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31244.1"
                     /translation="MKAEFVVVLVMVSILTFVGVSAYAHTPMLYVEDLKDGTVYLEGG
                     FSDGSSAAGVNIYLVEDAAFKGNTSARDRYLEAIFANAPKERAAYLAKLGSKVDVKSK
                     GFRYSDLIPELFEKKLIIFRSQLDQYGELVLAKPDGPYLIVFDAGPGHVVVKKGPALT
                     SAEKKNFR"
BASE COUNT         1933 a         2282 c         2067 g         2025 t
ORIGIN      
        1 tccctgccgt agtgatattg acgtccctca tggtgatggt gccttccatg gtcgcatcca
       61 caccatgggc tccgggcgcc tcgcatgcaa tgtccacgtt ggccatgtct atcgaagccc
      121 cggcaccgca cgcgaagacg gcattggcac cggttccgga ggtaatgatg gtcgagtcgg
      181 ttattgatac cctgctaccc gcctgggcca gaatcgccgc gttgaggccg taaaagctgc
      241 tgctgtccat ggacgaggtg ttgccggagg ttttgatcgt catgcccgag agattcagct
      301 gtgctccgtc cgacacgatt atcgccgact ggtcgtctgc ggaggcggtc agcgtctcac
      361 tggacatcgc cgccgatccg ctgcttagcg tgtagacccc tgtgccagtg atgtacgtgc
      421 tgccgacggg cggtggggca atctcggttc cgcctggccc gggcatgccc ggccgaaatc
      481 cgtcaccggg cggttccggg ggtgtgcccg gccccccgcc aggcggctgg cccccctgcg
      541 cgaccaccgc caagcccgac actgccagta ccatcaggac gaacataatg gcccgccaga
      601 gcttactcct tgtcatatgc aaacatctcc tcgtcattga tgggtacgat ccgatccgct
      661 gctgccgccg gacgatagta ttagccgcac agcttcacaa gaacgccccc ttacttcaac
      721 gccgcaacgg gggcaggcgt tccggtaccc gggcccatat acctcctgcg ggctttgatt
      781 catagatcta aaaaggcgcg ataaaggtct gcttcgcaga tgcctcgaag gtgaatccta
      841 ttgaccgcgg acaaaacgta atggtacact gataaacatg atgacacggc tggtcttgag
      901 cgaaaaaatg agccctggaa agcagaatgc ggaaaactag cagcagccca cttagagcaa
      961 gctttcatgt gggctcgaag aagcaagccg ccagcttgag ccgatcatgc ttctaccggc
     1021 taagtaacca cagtcgagta gccaagtccc gaacatcagg cgatgcaaat atggcagttt
     1081 tcccgtagca aaagcccagt gaatcacgtg tcgatatcag tacaatatca cacacgcccg
     1141 tgtggaatga ggtgaatatg ggtgagtcaa ccggaatacg cgtctccttc gcttgaggtc
     1201 tggaaggatc tgtacgaagc cgctgcgcaa ttcaagcgca ttgagagttg gaattggctc
     1261 gacgactcgt ccacctttgg agttcaagat cccgtcacaa agacgatagc ttattgctcg
     1321 gtcatgggtg ccctcggaga gctttacggt ctggcggcgt atgttggcac cgagggactt
     1381 ctggcgcttc agaagatggc gtttggcgaa accgactaca gcgacattaa tctcggcaaa
     1441 gacatgaggt gcctgatgac cacttttgag agccgaaacg atttggacaa gcctgatatt
     1501 gaagtcatac gccagctcgg cctctcgttt cgcggcaagc acaactggcc aaggttcaga
     1561 agttacgaac ctgggttttt cccttggtat ctagatgcgc acgaggcgat gttcgtaaca
     1621 acgatcttac agcaagctgt agacgtctgc cttcgggcca aagacgaccc cgatctcatc
     1681 cctaattggg atgacgaagt ctaccttgta agagtggctg aggaaaagga tggtgagctt
     1741 gtttgggaag atcggatgtt gtcgcccgaa ctactgcccg aacccgagct ggaagactcc
     1801 gttcacatcg atgagctcca gctgatgaag atccggaatt ctgcaaagac aacagatgca
     1861 gtctgggatg ccgatctttt ctacgctcct gcttgcgtcc aagaagatcc gaaatcccga
     1921 ccctactacc ccctaatgag cttgtgggtg gatcacggta cgggactgat aatagggaca
     1981 aagatggccg gccatgacga ctacagacag gcatttgttg acgaactgat caacatggta
     2041 aatcaagaca acgtaaagcc ccgggagatc agggtcaaaa gggaagatgc ctatcggctt
     2101 tacgaaggag tcgcttccaa actgggtata cccatgcgta aagtccagag cctaggcatg
     2161 ctcgagggca tccaggcaga tcttgcagag ttcttgatga gacgatagcg gaggggtatg
     2221 cgggagtgtc tgtagctcga ggaaatcggg gtaacaggat ttggaataca gnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggtgctcg gtaggtctat aaatgactgt
     2401 cgcacgctac cgcggttcag cgcgacgaac catcccgtta tctatcgcgt ttacttatga
     2461 tgtcgacaca atatgctata ttacttcttt ctgtctctgc aaagacgccg gtttttttgc
     2521 agatcctggc ctagccgttt tgaacaaacg gcttcccaca agtgctgtaa tgggaatggc
     2581 ggcaatgagg ccgatgcttc cggcaagtgc cttaactatt tcggtggcga tatggtcgaa
     2641 gttgacgatc tggactattg gagtttcata cgccaaaaag agcaataaaa gcgggatgga
     2701 accccctgca tatgccagta ttagggtgtt ggacatcgtg cccattacat ctcgccctac
     2761 ccttaacccc gagcgaagca actgctcctg cgtgattcgc ggccttgcca gcatgacctc
     2821 ttccatcgac gatgcaatgg acatcgctac gtccattact gcgcccagag cgcctatgat
     2881 cattccagaa aacagaagcc ggtcaaaatc gaactggact tcttggggta tgtaaaagag
     2941 catggttgct tcttctgttc cgaagccggt gaggtgcgct gctgagccca cgatcatcgc
     3001 gatcagtccg gcaatgatca ctcccaccgt ggtccctatc atggccgtca acgtcttttt
     3061 gttaaaacct cctacgatac cgagagtaat gacggtgatg gcgcttgata cgaggattgt
     3121 gacgtaaatc ggatcatacc ccctgagcaa aacaggaagc agaatccata ctatcgcgag
     3181 ccctgttact cccagtgtta agatggtttt gatgcccgta agcccgccga ccaaaagcag
     3241 cacgcccatg aatccgacca cgagccattt gagatagtgg ccgcgatctg gagagaagac
     3301 gtgggcttca actatgttgc cgtcgaggat ctcggcccag actatcactc tatcgtttgg
     3361 ttcaacatcg aagtcgtaag cagggtgccc tgatttcaga gaagtcgact gaactactac
     3421 gcctttgtat ggtgctgatg tgagctcgag cttgatgacc tccgattcca ctgcaaaatc
     3481 gtcaggaacg gcgtaatcac tgatgacttt ttcgacactg agcactctgg ccttcaaaaa
     3541 ctgaggctcc attccatccg gagggtagtc ggcgtattct tgcgcatgga ccgcagtgca
     3601 ctccagcgca agcagcgtca ttatgagcaa agcaataata cctgatctct ttttcaaccg
     3661 tgtttccccc aatcgcagga agaagcgcat ccgctgcatc ggcctgattt tttacaccta
     3721 gggggctttt tcaatttccg aataagcaag tatgctgcca atgccccgct agctgcgatg
     3781 attgcggtac cgctcaatgc cttaccctcc tttcaagtaa atccatattc tgtgctaatc
     3841 agcaaatatc ctgctgattt cattgtctcc tgctatcaat cgaacggcca gccagcgcgg
     3901 gtcgtaaccg gatgccctac tgtcgccggc cagagcttct tccagcactt caagagatcg
     3961 gttgagctgt gggccatagt ccaggcgaaa gtcgttggcc tcatccctgc attcgtaggc
     4021 ttttagcaag gcatcgcata gttcgggttt tcctatgccc tttttggcca cagttgggac
     4081 cgctcttacc cccaacttct tgctgaggcg ttcgacgtct atctctatgc ctgcgttttc
     4141 ggctgcatcc atcatgttca gcgccacaac cattggcatc ctcatctcga ggagctgaaa
     4201 agtgagtacg aggttgcgct caagatttga ggcgtctaca acatttagga tcacatccgg
     4261 tttttcgttg agcaggaaat cccgggcaag cctctcttcc aaactgtagg aagtcatgct
     4321 gtatgtaccg gggaggtcca ccagcgtgaa cttgtgaccg ctatagtcca atcggcctac
     4381 acgtttctcc actgttaccc ccgggtaatt tgcagcaaat tggcgcaagc cagtcaacat
     4441 gttgaatata gtggactttc ctgagttcgg ttgaccggct actgctataa ctatctccgg
     4501 catagcggaa tcacctcgag tcttctcgcc tctgagtggc ggatgctcac gtagttgttc
     4561 ttgacgagca attcaactgg gtcgaccagg ggcgcatttc gcaccacctt gatttcgact
     4621 ccgggaataa ggcctaaatc catgaaccgc tgtgcagttg catcattggc ctttatgtcg
     4681 tgaagacggc acgtatcgcc cggtttcagg ctatccagtc gtacagcgtc attcatcgat
     4741 catcacatct ccttagctac aagtattttt ttcgccactc ctctgcccac tgcgatcgtg
     4801 cagtcgttta cctgcattaa tactggacct gctcctgccg agcttatcac catcacttcg
     4861 gatccatcgt acaggcccat gtctgacatt cgtctagcaa ggcctctgcc ataattcccc
     4921 acattaacca ctgttccctt gcaccctcta ggaagctcgg tcaatggcat aatcgtttta
     4981 tccacgcaaa ctccctcctg caactatcta ttgatcgaac tcgtcttcgc ttggcccctt
     5041 aaccgttgcc aaaaccagca tcaacatgat cgagatcgcg taaaatctga tcatggcctg
     5101 taagcctgac agaccgagcg cgttcccgcc ggtgaatatg agactggcca gcaacaagcc
     5161 gagcagcacg ggatagaatg ctgcaagcag catccacatc gtacttcccg tctccatctt
     5221 tactgccaac aatgttggta tgcaaggcgg gaagaccacc atgaagacca tcaaagcgag
     5281 tgcatgcaag tcggtccagc caagatcgcc ttcagcgacg ctctcctcaa gcctgacccc
     5341 ggtgtcgagt ccatagatac ttcccaaagt tgcgacgctg ctctctttcg ctgcaaatga
     5401 actgatcagt gctatgttga ctttccagtc gaaccctgca tacttggtga ccggttcgag
     5461 cgccctgccg actatgccca aagcgctgtg gacgatgatg tcatcacttg cgcgagctct
     5521 tagggctttt cgctctcttt caaaggatct gtaagccctt gatattctgg cggcttccgg
     5581 atccacttcg atcatcccat tcgtttcgaa tcttcctctg ttcactaact tatagaactc
     5641 caaattcttg gccatgaaat ccttgtgcac tttatcgagc ttggcttcgc tccttgcaga
     5701 tttctttccg gcatcgaatt gattgctgaa atcgatcaac tctactatcc cattacccga
     5761 taggagctgg gcgtacacgg aatcgtcgtc cactgacgcg agaaaagcat cgaccatctt
     5821 gtctgcttct gcggaatagg ccgtcttctt gcttgcgttc aagcctggta agttggtaag
     5881 ggcgaataca acgatcatga ccagaagaac cacggatacg atttttctaa agaacatcca
     5941 caaccgttcc caacagcgtt ggaagactcc tctgatggta ggcaggcgat aggcaggcat
     6001 ctccattacg aacggagccg attccttgac tttcaatacc gtcagagaca gaagtttgct
     6061 cactgtcaac gcgattatca agctgatggt ggatatgaag aagaccgcaa agcccttgtg
     6121 ctctccgtag aacatctcta tcaggagtat gtgcagagga gtcttcgcca ggcagttcag
     6181 aaggggaatg ataagaatgg tggcgagacg tgcccgctga tccttcattc ctctcgtagc
     6241 catgatggca gggacagcgc atcctccaac tactacgccc cccaaaacca tgggaaacac
     6301 agattgtccg tgtagtccga actggcgaag tattctgtcc ataataaagg ccatcctcgg
     6361 cacgtaaccg acatcctcca agatcgctac aagactgaaa agtattgcaa atattggaac
     6421 ataattgata acagccagga gtccatcaac gacactcagg accatgtccc tgacggtcgg
     6481 gtcaaacagt attccctccg ggggcagtat ctgtgctacc caattccgaa acccggcaag
     6541 caaaggccag gtgtagttgg tcaattgata ccctttagca atgctaaggt aatagagaac
     6601 gtacagtatt ccagctagga tcaggggacc gaagaaacgg tgcagcacga tgcggtctat
     6661 tctgttgctg aggctctcct tctgctttgc ctctctgact gcacactcgt caaataccct
     6721 tgaggcctgg cgatagtatg ccgtagtcac cagcctcgcc ggttcacttt gctcttccct
     6781 caagagccgt tcccgttctt cagctacaag ctgcaatagg ttcatgatgg gcctccttcc
     6841 gtgtggatgg ggcatcctgt gcgatagcgg atgccccaaa ggctcgcgtc aactactcca
     6901 tgtcatatgc ttttacccag atcacagcgc caatctcatg gtccttgcca tcaatgcttt
     6961 caccttccat gagggcggca aatccccacc agccggactt gggaattccg taagtgaaaa
     7021 ccccgtttcc atcggccctg attatctgag tcacgtgcgc ggacgacgga tacgtctggc
     7081 ctaagcctgc gaagctgctg aaagcatccg cgttgagcct ctccacttcg atttcggcaa
     7141 acggaactgc cttgccgttc tgctttacga ctccttgaaa gacgttgccc gtccaaaggc
     7201 cgtacggctt gcaaagaggc acaatttcgg cctccaaacc gatctcgaca tcccagtctg
     7261 tggggaatcc agcgttattt accacgactt tggtgtattg ggttatgtag gaatcctcca
     7321 tgctttcata gtatgggctc ggccggaggt agaatacgaa gtcaccggcg cctctcgctg
     7381 tgaattcggt ctgccatgcc tttgcaccgt gaaactcaaa cgcttcaaga gtatcggtga
     7441 gatcacgcgc ttttcccttg tgatacactc caaattcaat cggcttatcc atgttcatgg
     7501 tgtgcgatgc ctctgcagga tgggtgaata tcagctgcag ctggaccacg ttgctttcgt
     7561 tctcctctat gatgtcatct gaaggcaaaa ccatctgaaa atgcgccatg gaactagcgg
     7621 aatataccgc aataatcgcc agagccattg tcagcgtgta taccacccta ttccatggtt
     7681 tgtgcggtcg ggccattttt tgttcttcct cctctaagca attacctgaa gtttttcttt
     7741 tctgcgctgg tgagcgcggg gccctttttg acaactacgt ggcctgggcc ggcgtcgaac
     7801 acgatcaggt atggtccgtc cggttttgca aggactagct cgccgtattg gtcgagctgg
     7861 gaccggaata tgatcagttt cttctcgaac aattcaggaa tcaggtccga atagcggaat
     7921 cctttggact tgacatcaac cttgctacct aattttgcta gataagcggc cctctctttt
     7981 ggggcgttgg caaaaatcgc ttccagatac cgatcgcgcg ccgacgtgtt tcccttgaag
     8041 gcagcatctt cgacaaggta aatatttaca ccggcagcgg atgatccatc ggagaaccct
     8101 ccctccaggt atactgttcc gtctttcaga tcctccacgt acagcatggg tgtatgagcg
     8161 tacgcagata ctcccacaaa ggtaaggatt gacaccatta ccagaaccac cacgaactcg
     8221 gctttcatag ggtcgacctc cttggtatga tctgatcttc cccgagcact gttgtgccta
     8281 gggatgaggc aattggccac catgttaggt gtgcctaact tttgatcagc agtgctcctg
     8341 tttgaggcag ggtacgcccc gcatatcatg gtagactgct gagcagttca tccaatcgca
     8401 taagtta
//
LOCUS       DAOSYL010000080         8384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7235_length_8384_cov_7.620483, whole genome shotgun sequence.
ACCESSION   DAOSYL010000080 DAOSYL010000000
VERSION     DAOSYL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_8384_cov_7.620483"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLU15_09800"
     CDS             complement(<1..651)
                     /locus_tag="PLU15_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31245.1"
                     /translation="MGSAHARLWVPGAMFAALLAARFVYGWIVPTAGVWWVAAIDASL
                     VFCSAFIAAYYARALKSQLDAVEAVSDRAGRLELVARELVDNEAVMREDLVRGQRELN
                     ERILRLSSLLDATRELVGARNLEDALQHVADMTCKVMGADKATISVTMADEALTVVST
                     GTNCSDSLLFRSSSEIVLFGDAAGELVVEFANISPGNADAGSFLSIISAFASAAIEN"
     gene            complement(731..2374)
                     /locus_tag="PLU15_09805"
     CDS             complement(731..2374)
                     /locus_tag="PLU15_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO31246.1"
                     /translation="METGMRDDVKAPARRVLIVDDETNMQVVLSAAFEDAGFATGTAS
                     TGEEAIRALTQTDYDLMLLDLKLPDMTGIDVFRHAREASPGTIVIMITAYSSVETAIE
                     AMKMGAYDYITKPFNLEKLLMIAGNAIDARRCARIDSGSSAGLLPSTRLVRGGLERNA
                     MRGDTAKAGSAVAFHGGKAAGTLSQADWALSGIELVAEDEDFDGVVGVTPQMRQLMEM
                     SRDIAPTNATVLIYGESGTGKELVADYIHKKSLRAGRPFIKVNCAAIPEPLLESELFG
                     HEKGAFTHAISRRLGRFELADKGSILLDEVGEMSNAMQAKLLRVLQEKEFERVGGTET
                     IKVDVRVIAATNQNLKEAVLRGDFREDLYYRLSVVPLFLPPLRDRKRDIPLLCARFID
                     KYNKEFGREVSGVSPKVLEMFAEYDWPGNIRELQNAMERAVLLSRSDVLTPDDFHLGM
                     DPAEIISLGTGVTCRSCANSRLAGRNWDGSGADMVSDGADGYIEILDENGSPLSLEEM
                     EKRHIKAVLDQFNWNKTQAAQALNITRRTLLNKINKYDL"
     gene            complement(2564..3061)
                     /locus_tag="PLU15_09810"
     CDS             complement(2564..3061)
                     /locus_tag="PLU15_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOO31247.1"
                     /translation="MAHELLSDIASLEERIHSCRDEQLTLYKRLLELSDAQRGAIARG
                     DTDALLWATEEKGRLIERIDAIDIRLAGLIDDASILAGKEGLSHLCPYKEPGIDSACP
                     LNVKIAQIIKTILETDRENQAILEEAIGAAGDEIKQLDSGGKAVKAYWRRSPESPAGS
                     VDDVF"
     gene            complement(3061..3531)
                     /gene="fliS"
                     /locus_tag="PLU15_09815"
     CDS             complement(3061..3531)
                     /gene="fliS"
                     /locus_tag="PLU15_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOO31248.1"
                     /translation="MMECRAANGRDGGSMMTSAYNRYMEVQVQTAPPENLVLMLYDGA
                     IRFATQAKTDFAEGNREAAHNNLTRAQDIMGELMGSLNMEIGEVAGNLFSIYEYMQHR
                     LIEANIRQSVECIDEVLKMLGELRQTWADAIRQYRGQSAVSADSVGGGERGEVG"
     gene            complement(3528..5636)
                     /gene="fliD"
                     /locus_tag="PLU15_09820"
     CDS             complement(3528..5636)
                     /gene="fliD"
                     /locus_tag="PLU15_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOO31249.1"
                     /translation="MAGDFRVSGLASGYDTAQLIEQLIALERKPVLQMQKRQDQIKVR
                     SDAWRDITTRLSNLKSKASSLKLEATINAKSASSSNDKLVTATASPGAANAVYKIWVD
                     QLATSTKVASGGQVGRELDLKVKLSEAGFATMPTVGTFTVNGVAIRIDTDTVMSDGVD
                     DAGSNSILGKINSSGTGVTASVEDNRLVLRAQPGSGPIRLGSGGDTSNFLTAAKVLGV
                     PAGETVSSYGVLGVAKTTVALNKARLATGIAHSVTGGVVEGETEGKLATALDEGTTVV
                     FTYRGQEYTTGALSGGDDMELIAAELEAKMNEAAGLEPGTIRVRVSGLEGKRNDRFVI
                     TDTQTPELGDGADGVADNSIIVNEASSALRLTISEGATTKGMFKLNGVAIEYDANRDA
                     INDIINRINASTAGVTATYDVLSDSLVLASKETGGLTITMDDVGGNFLSAVGLAGAEQ
                     EYGKNALFRVDGVQDGAQLSSASNTVSGILTNVTLTLKDVGEDPVTVTVFQDTESAAK
                     AIRGFVDQYNSTMDLLSSLTAYDKETKTAGALQGNSAVRGIQTSLRRLTMASVEGQPK
                     GFNSLLAIGISTGAPGRKVGSSTGTLTIDEAKLKEALTSDPQSVLRLFNEAEQGIAVQ
                     YEKYIDELVKSTSGLISQTQKVLKEQTKDIDRRIEEMEARLEKRKESLIRQYTQMEKA
                     LAAMYQQQMWMDAQFKSMGV"
     gene            complement(5654..>5903)
                     /locus_tag="PLU15_09825"
     CDS             complement(5654..>5903)
                     /locus_tag="PLU15_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOO31250.1"
                     /translation="WNWQTPRRQQDDVRAQVRRANKALEALEVQAKFSVHEGTGQIVV
                     KLEDTQTGEILREIPPEKMLDLVAQMTEMARGIINEKP"
     assembly_gap    5904..6003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6049..6738
                     /locus_tag="PLU15_09830"
     CDS             6049..6738
                     /locus_tag="PLU15_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOO31251.1"
                     /translation="MKKLVVLTGAGISAESGIRTFRDSDGLWYEYDVMDVATPEGWRR
                     DPKLVLDFYNQRRRELYRTKPNAAHLALARADEDFDVHIITQNIDDLHERAGSAKVLH
                     LHGELKKSRSTADPSLIYDIEGTELDWGDKCELGSQLRPHVVWFGEPVPAMAEAAEIA
                     QSADIFAVVGSSLVVYPAAALVQFVPSDAPVFLVDPKEVALSSLREVTHIMEKATTGV
                     PKMLESMKKLG"
     gene            complement(6778..7080)
                     /locus_tag="PLU15_09835"
     CDS             complement(6778..7080)
                     /locus_tag="PLU15_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOO31252.1"
                     /translation="MVRHVVMWSLADEAAGGVKQQNAAIIKEGLEALVGKIEGLIGLE
                     VGINALELAGNYDIVLIADFQDEAALERYQVHPEHVKVADFIKKVRLERVAVDYLM"
     gene            complement(7147..7377)
                     /locus_tag="PLU15_09840"
     CDS             complement(7147..7377)
                     /locus_tag="PLU15_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31253.1"
                     /translation="MNRTGSETPIRIEVEATGGTSVAAGDRVRCGQDLGISPDFSERV
                     VCHVDGLVEACVFNSDTHRFEITIVPDNAKKE"
     gene            7850..>8384
                     /locus_tag="PLU15_09845"
     CDS             7850..>8384
                     /locus_tag="PLU15_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31254.1"
                     /translation="MDLRQPSARQRQIVTTWPVRVESEIAKSIRNDRWNVPYLGEWLM
                     TCWLDYLVVSADYLDHSRPEWLRQTSTGHKMEFDREYKGLRTAFEFQGQQHFQTTLMY
                     PDSLALVEQQRRDNTKAGICARHGVRLVEVTAEDLNLRVMLQKVAWLPLRQYRQRGPI
                     VSELTQIYDSYMGHLAKT"
BASE COUNT         1867 a         2464 c         1999 g         1954 t
ORIGIN      
        1 attttcaatc gctgcactag caaaagcaga tatgatagac aagaagctgc ctgcgtcggc
       61 attgcccgga ctgatgttgg cgaactccac taccagttcg cctgctgcat cgccaaacag
      121 gacaatctca gaagaagagc gaaagagaag ggaatcgctg cagtttgtgc ccgtggatac
      181 gaccgtaagg gcttcatctg ccatagtcac acttatcgtc gccttgtctg cgcccattac
      241 cttgcacgtc atatcggcca catgctggag agcatcctcc agattccttg cgcccacaag
      301 ctcccttgtt gcatcgagaa ggctcgatag cctgagaatc ctctcattca gctcacgctg
      361 gccacggacg agatcctccc tcattacagc ctcattatcc acgagttccc tcgccacaag
      421 ctcgaggcga cccgcgcgat ctgaaacggc ctccaccgca tccaattgag acttgagagc
      481 ccttgcgtag taagccgcga taaatgctga gcagaacacg aggctggcat cgattgccgc
      541 cacccaccaa accccggcag tcggaacaat ccagccataa acaaatctgg ccgccaaaag
      601 agcggcaaac atcgctccag gcacccatag tctagcgtgt gcgcttccca tggctgtcac
      661 accctcatta aaaatacctc tcatcgacgc tagacagcag tgactgtgtc cgacgcgcct
      721 gctcgagagg ctacaggtca tacttgttta tcttgttgag cagagtccgc ctggtaatat
      781 tgagggcttg ggctgcctga gtcttattcc agttgaactg atccaaaacc gccttgatgt
      841 gtcgcttctc catctcctcc aaagacagcg gactcccgtt ttcatccaaa atctcgatgt
      901 agccgtctgc accatcggac accatatccg ccccactgcc atcccagttt cgcccggcca
      961 gcctcgagtt ggcgcagctg cggcaggtga cccccgtccc cagggatatg atttcagccg
     1021 ggtccatccc aaggtgaaaa tcatcaggcg tcaacacatc acttcttgaa agaagaaccg
     1081 cccgttccat cgcgttctgc agctcgcgaa tattccccgg ccaatcatat tcggcaaaca
     1141 tctccagcac cttaggggaa acacccgaga cctccctgcc gaactccttg ttgtatttgt
     1201 cgataaacct cgcgcacagc agtggaatat cacgcttccg atctcgcaga gggggaagaa
     1261 aaagaggcac aaccgacaat cgatagtaaa gatcttcacg aaaatcgccc ctcagcaccg
     1321 cctctttaag gttctgattg gtagctgcaa tcaccctcac atctaccttt atcgtctccg
     1381 tgcccccgac tcgctcgaac tccttctcct ggaggaccct gaggagcttg gcctgcatag
     1441 cattggacat ctcgccgact tcatcgagta atatggaacc cttgtcggca agctcaaacc
     1501 tgccaagccg tctggagatt gcatgggtaa aagcaccctt ctcatgcccg aaaagctccg
     1561 attcgaggag gggttcggga attgcggcgc agttcacctt tatgaagggt ctgcccgccc
     1621 tgaggctctt cttgtgaatg taatccgcaa caagctcttt ccctgttcca ctttcgccgt
     1681 atataagaac ggtggcattg gtgggtgcta tatcccgcga catctccatc aactgcctca
     1741 tctgcggcgt aacgcccaca accccgtcga agtcctcgtc ttcggctaca agttcgatgc
     1801 ctgagagagc ccagtcggct tggctgaggg tccctgcagc cttgcctccg tgaaaagcca
     1861 ctgcactacc ggcctttgcg gtgtccccgc gcatcgcgtt ccgttctagg ccaccacgca
     1921 ccaaacgtgt agacggcagt aggccagccg aagagcccga gtctattctt gcacatcgcc
     1981 ttgcatcaat ggcgtttccc gcgatcatga ggagtttttc caaattgaat ggcttggtta
     2041 tgtaatcata agcgcccatc ttcatagcct ctatcgcagt ctccactgaa gaataggcgg
     2101 ttatcattat cacgattgta cccgggctcg cctcccgcgc gtgcctgaac acgtcgattc
     2161 ccgtcatatc aggtagcttg agatcgagca acatgaggtc ataatctgtc tgagttaggg
     2221 cacgaatagc ctcctcccca gtcgatgccg tgcctgtcgc aaaacccgca tcttcgaaag
     2281 cagccgagag aactacctgc atatttgtct catcatcaac aataagtacg cgcctggcag
     2341 gcgccttcac atcgtccctc atacccgttt ccacaacatt gtcccccatc tgaagagatc
     2401 cgaggcactg ttccatataa tggaacaacc tacaacccca ttacaattct tcagatagtt
     2461 acatatatcc ttcttatttc agcaaaaaca gcatgggcac caagggatat atgcgagacc
     2521 gtgcaaagaa cggtgagaaa gtcggcggcc taacgcggaa aactcagaag acgtcgtcga
     2581 cgctgccagc aggagattcg ggagaacgtc tccagtaggc tttgaccgct ttgccgcctg
     2641 aatcgagttg ctttatctca tcgccggccg ctccgatcgc ctcctcgaga atggcctggt
     2701 tctccctatc tgtttctagg atcgtcttga tgatctgggc tatcttcacg ttgagcggac
     2761 aggcagaatc tatgcccggc tccttgtatg ggcaaagatg tgataggccc tcttttccgg
     2821 caaggatcga ggcatcatcg attaggcccg ccagcctgat gtctatcgca tcgatcctct
     2881 cgatcagcct ccctttctcc tcagtcgccc agagaagagc atcagtgtca cctctggcaa
     2941 tagccccgcg ctgggcatcg gaaagctcaa gcagtcgctt gtagagagtg agctgctcgt
     3001 cgcggcatga atgtatacgt tcctctaatg aagcgatatc agaaaggagt tcatgcgcca
     3061 tcagcctacc tccccccgtt cgcctccacc cacagaatct gcgcttacgg cagattgtcc
     3121 ccggtactgc ctgattgcgt ccgcccacgt ctggcggagt tcaccaagca tcttgaggac
     3181 ctcatctatg cactcaacag actgtcttat gttggcctca atcagtcggt gctgcatgta
     3241 ctcgtaaatg ctaaaaaggt tcccagctac ttcgccgatc tccatattga gtgagcccat
     3301 gagctcgccc attatgtcct gggcccgagt gaggttattg tgggcagcct ccctattccc
     3361 ctcagcaaaa tcggtcttgg cctgagttgc gaaccttatg gcaccatcat atagcatgag
     3421 gacaaggttt tcaggtggcg ccgtctgaac ctggacttcc atatatctgt tataggcact
     3481 ggtcatcatc gaaccgccat cccttccatt ggccgccctg cattccatca tactcccatc
     3541 gacttaaact gcgcatccat ccacatctgc tgctggtaca tggccgcaag cgccttttcc
     3601 atttgagtat actgtctgat caacgattct tttcgttttt cgaggcgagc ctccatctct
     3661 tctatccggc gatctatgtc ttttgtctgt tccttcaaaa ccttttgagt ctgagatatc
     3721 agccccgatg tgctcttcac aagttcgtca atgtactttt catactgaac cgctataccc
     3781 tgctccgcct cgttgaagag cctgaggaca gattgtgggt cgcttgttag ggcttccttc
     3841 agcttcgcct catcgatggt gagcgtaccg gtgctggagc caaccttcct ccccggagcg
     3901 cccgtggata tgcctatcgc cagaagcgaa ttgaaaccct tgggctgccc ttccacagag
     3961 gccattgtaa gacggcggag agatgtctga atcccgcgca ccgccgaatt gccctgcaga
     4021 gctccggccg tcttggtctc cttgtcgtac gcagtcaagc tcgacaggag gtccatagtg
     4081 gagttgtact gatccacgaa tccacgtatc gccttggcag cagattcagt gtcttggaag
     4141 actgttacag tgaccggatc ctcgcccaca tccttgaggg tcaaagtgac attagtcaga
     4201 atcccggaga ccgtgttaga cgcagatgaa agctgagcac catcctgcac cccatctacg
     4261 cggaagaggg cgttcttccc gtattcctgc tctgcgccgg caaggcccac agcagagagg
     4321 aagttgcctc ccacatcatc catggtgatc gtaagacctc ccgtctcctt tgaggcgaga
     4381 acgagactgt cagaaaggac atcgtacgtg gcggttacac ccgcagtcga tgcgttaatc
     4441 cggtttatga tgtcgtttat agcatcgcgg ttggcatcgt actctatagc aaccccgttc
     4501 agcttgaaca ttcccttggt cgtcgcgccc tcggatatcg taagacggag agcagacgaa
     4561 gcttcattca caataatgga gttgtcggca acaccatccg ccccatcacc caattcaggc
     4621 gtttgggtgt cggtgatgac gaacctgtca ttcctcttcc cctcgagccc tgaaacgcgg
     4681 accctgatcg tgcccggctc aagccctgcc gcctcgttca tcttcgcctc aagctccgcc
     4741 gcgatcagct ccatgtcgtc gcctccggat agggcgccag tagtgtattc ctgacctcga
     4801 taggtgaaca cgacagtcgt tccctcatcc agcgccgtcg caagcttgcc ctcggtctcg
     4861 ccctccacaa caccgcctgt gaccgaatgg gcaattccag ttgccagcct cgccttgttc
     4921 aacgccaccg tagtcttggc cacgcccagc acaccataac tcgatacagt ctcgccggcg
     4981 gggacaccaa ggaccttggc cgccgtaagg aaattgctgg tatcgccgcc gcttcccagc
     5041 cgaatcggtc cggacccggg ctgggcacga aggacaagcc tattgtcctc gaccgaggcc
     5101 gtaacgcctg tgcccgagga attgatcttt ccaaggatcg aatttgagcc agcgtcgtca
     5161 accccatcgc tcatcacagt gtcggtatcg atgcggatag ccacgccatt gacggtaaac
     5221 gtcccaacag ttggcatcgt ggcaaaaccc gcctccgata acttgacttt caggtcaagc
     5281 tcccgaccga cctgcccacc cgaggccacc ttagtggaag tggcaagctg atccacccat
     5341 atcttgtaga cagcattggc agcacccgga cttgcagttg ccgtgaccag cttgtcgttg
     5401 gacgacgacg cggactttgc atttatcgta gcttcaagtt tcaatgaaga ggccttcgat
     5461 ttgaggtttg aaagacgagt agttatgtcg cgccaagcat ctgaccttac ctttatctga
     5521 tcctgtctct tctgcatctg aagcacaggc ttgcgttcaa gcgctatcaa ttgctctatg
     5581 agttgggcgg tatcatagcc cgacgccagg ccggaaacgc gaaaatctcc tgccaccgtc
     5641 attcacctcc aattcaaggt ttttcgttga ttatcccacg agccatctcg gtcatctggg
     5701 cgacaaggtc gagcattttt tccgggggaa tctccctcag aatctccccg gtctgcgtat
     5761 cctcaagttt gaccacgatc tgcccggtgc cctcgtgaac cgagaacttc gcctggactt
     5821 cgagcgcttc gagcgccttg tttgcccgtc gcacctgagc cctcacatca tcctgttgtc
     5881 ttctgggcgt ttgccagttc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnataaaca cggtgtttgg cttggaggaa gggaatgata ttcgacgcat gaaaaagctt
     6061 gtggttctga ctggcgctgg tatcagcgct gagagcggaa taaggacttt ccgagattca
     6121 gacggtctct ggtatgaata cgacgtcatg gatgtagcga ccccggaggg atggcgtcgt
     6181 gatcccaaac tggtcctcga cttctacaac cagcggagaa gggagctcta ccgcacaaag
     6241 ccaaacgctg cccatttggc gcttgctaga gccgatgagg acttcgacgt acatataatc
     6301 acccagaaca tcgacgacct ccacgagcgg gcaggcagcg ccaaggtgct tcatctccac
     6361 ggcgaactga agaaatcgcg ttccacagct gacccatcac tgatctacga catagaaggc
     6421 accgaactag actggggaga caaatgcgaa ctgggatctc aactcaggcc gcacgtggtc
     6481 tggttcgggg agcctgtgcc ggcaatggcc gaagcggcgg agatcgccca gagcgccgac
     6541 attttcgccg tagtaggcag ttccctcgtg gtctaccccg ccgcagcact ggttcagttc
     6601 gttccttctg acgctcctgt gtttctggta gatcccaagg aggttgctct ttcatcgttg
     6661 cgcgaagtca ctcacatcat ggaaaaagca actacaggtg tgccgaagat gctggagagc
     6721 atgaagaagc tgggctgagg ccggaaatct gtcgggtatc tgtatctgtt atggatgcta
     6781 cattaagtaa tccacggcaa cccgctccag cctgaccttc tttatgaagt cggctacttt
     6841 cacgtgctcg ggatggacct gataacgctc caaagcagct tcgtcctgga aatcagctat
     6901 gagcactatg tcgtagtttc cagcaagttc gagggcattg atgcctactt ccaggccgat
     6961 taagccttca atcttcccga caagggcttc aagcccctcc ttgataatgg ctgcgttttg
     7021 ctgcttaacg ccgccagcag cctcatctgc gaggctccac atgactacat gccttaccac
     7081 ttttctagtc ctccttcggc cgctcagcca gttctcatca aaccgcattg ctgatgcaag
     7141 tctactctac tccttttttg cattatcggg aacgattgtt atctcaaacc tgtgcgtatc
     7201 agagttgaag acacaagctt ctaccaaacc atcgacatga cacacaacac gttcactaaa
     7261 gtcgggggaa atgcccaagt cctgaccaca gcgcaccctg tcgcccgctg caacagaggt
     7321 tccacccgtc gcctcaactt cgatccttat tggcgtctct gatcccgtcc tattcacagt
     7381 ttcagccact cctttttcgc ccggttttca agacccgcag agttttttga caagccaact
     7441 acatgatagt ctgcaagctt gatttctcca ttcagtgcta aaggccttca ccttaggccc
     7501 ttctcgaagt atctgcccgc aacgagatgt tctttcaggc taaccaggat gacaaacgtc
     7561 tgaccatatt gcctgcacga cccgtggatg gtattatgtg gataatatca atacgttcct
     7621 gacctgtttt ttcgtgaggt gaaatgcgtt gtccttccga cagcacattc agcacacaat
     7681 cccagaagct ctgttcaatg atactcgagc ctgtccacgg gctcagagtg tctacctgtt
     7741 cctgattgga aggcgcccgg aatcaatcag caacatcgct gcaggcattg gctcatcact
     7801 ggactcggca cgagcagcgg ttgcacatct aaaatccaaa ggatgggtta tggatctaag
     7861 gcaacctagc gctcgccaga ggcaaatcgt caccacctgg ccggttaggg tggaatcgga
     7921 gattgccaag tcaatacgga acgacaggtg gaatgtcccg tatcttggcg aatggctgat
     7981 gacttgttgg ctcgactatc ttgtcgtgtc agctgactac ctcgaccact cgaggcctga
     8041 atggcttcgg cagacatcaa cagggcacaa aatggagttt gatcgtgagt ataaggggtt
     8101 gagaacagct ttcgagtttc aaggccagca gcacttccag acgaccctga tgtatccgga
     8161 ttccttggca ctggttgaac agcaacgccg cgacaatacg aaggcaggga tttgtgcacg
     8221 gcacggtgtc cggcttgtag aggtaaccgc agaagaccta aaccttcgag taatgctcca
     8281 aaaggttgcg tggctgccac tgcggcaata tagacagcga gggccaatag taagcgaact
     8341 tactcagatc tacgatagct atatgggtca tctcgccaaa actc
//
LOCUS       DAOSYL010000081         8144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7511_length_8144_cov_8.588577, whole genome shotgun sequence.
ACCESSION   DAOSYL010000081 DAOSYL010000000
VERSION     DAOSYL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7511_length_8144_cov_8.588577"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1608)
                     /locus_tag="PLU15_09850"
     CDS             complement(46..1608)
                     /locus_tag="PLU15_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO31255.1"
                     /translation="MTERSESDDVAISLDISCCGGPAVDDLAWDSGNRNGCDRGAVSE
                     VGSCEMKDDSVQESGGASQGIFSGVDLDEMMSHPSAMRKSILALAWPVVTELVLQTVT
                     QMADMIMVGRLGSQCVAAVGLSNQPFNIIQGCFMGIGVGTTALVARFMGAGDEGRADR
                     VTAQSLAIGSILAAVFAVFGYYFTPNFVRFMKAGPDVVPYAESYMRVLMPGLFMLVIN
                     MIASSALRGAGDTRYPMRVNIVLNLANIVGNYILIYGKFGAPALGVTGAALSTTIART
                     IGTAMLLWHLFSGKGRISVRTQGERFRFDPVLIRRVFAVGLPASLERVSLSLGISFYV
                     RIVSALGTAAYAAHAIAINAEGISYMPGFAFAVAATTMVGQALGADRPDVAERAGIES
                     WKMSATIMGLMGVVLFCFPDALMRLYGDDLEIISMGAEVLRIMAVAQVPMATSYVFVG
                     ALRGAGDTRAVLIFTMACVWGVRFCLAWLFVNVFGWGLAGAWYAMAIDWVARGTIAAL
                     RFRSGHWKQMRI"
     gene            complement(1800..2855)
                     /locus_tag="PLU15_09855"
     CDS             complement(1800..2855)
                     /locus_tag="PLU15_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31256.1"
                     /translation="MKDSKKWTVAALLVTVFLFSSSVANAASITVVEGTPVFFGGLSV
                     MHSEDGPLNLELNWLGIASDLDWEGATLRIGGAICPSLPAFSEGSAAYTFGVGAEMAR
                     VFSLMAAAEGSVMAYLQDCGDGPVMALVGQATGSTARIGLRTNFGVVPSGFDEQPARL
                     IGQSDWSDEPGRDLTGRGWIDVVGALQSSRTRADSWKIGYMRELGESDWRTDIGYIAR
                     YSPEDLGVAIKFYSGLAFGEPRVMLLSKLTFRADVTDSSGAEIDLVVQGGGGSEYAWV
                     SGTGWLSLGDRADLKLRICAPIMSEHEYDGPELFEPIDWAAFAVGLDFNEGIISSVTA
                     RYDLRTKAIGLELASRF"
     gene            complement(2845..6114)
                     /locus_tag="PLU15_09860"
     CDS             complement(2845..6114)
                     /locus_tag="PLU15_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO31257.1"
                     /translation="MNIVERSVNRPVTVGILVAACILFGVLSLDRIGLDLLPDINLPM
                     AAVVTVYPNADPETVESLVTAPIERALRSVRNIKDVTSTSMENVSFCFCEFNWGSDLG
                     AAQEDMRVKIDTMKHELPEGTQSPIVTLLDPSQIPMMMVTVGMPGDVGDVTQATREVV
                     KPAVERVGGVAGVSISGGADRVISVEYDHEKLVESGLSPLLLQQLITYQNLSIPAGVV
                     IDDGVRYQTRVGAKFTSADDIADLAIGLKKPTESPDGLGGFLGLSFFMPTFLTVGDVA
                     KVEETFEKAEGYTRVNGKPAVIMMIYKQSGENTVTVARQVREALAQLGQEHRAIEIGY
                     VFDQSTFITSSIDSLAENAGLGAVLAIAILFLFLRHFGSTLLVAISIPLSMIFAFVMM
                     YAFDLTVNLLTLGGLALGVGMLVDNSIVVLENIFRRQELGESPREAAIQGTAEVSRAI
                     TASTLTTIAVFLPVVFIQSMAGHIFRDLAMTVTFSLLASLIVALTVLPLMASSFVRVS
                     DSYIERAKRDRKRKESLGGIYDRMLDWALAKRWWVLGAIGIALVVALFAFPRLGTEFL
                     GSMDMGRVDVGLHLPPGTPVEVTNEVSLRVENELMKIPGIENVTAEVGSSGSGDFVAM
                     ASGGSANQASVAVKLLREGETMTSVAEAGAEIREILSDIKLDYPDLEYSVDTSGYGAL
                     TGGSSDYLSNSITLEIAGTDPKEISDYAARIAERLRKEPGFVEVASSTKETQPVILLD
                     VNRTRALMGGLTVGQVGLGVRTGMFGSTVTYVERNGELIPVIVRPASDGVPDLDSILN
                     MPITSSLSSASLLEGASGLGSNVSAAARTSSSAISTMLAPSNEVLVGKVVTPEIIDGP
                     MTISRRNGRRYALVQIAFEGMKLSQAGSLALSAAHEVEAPDGCSVELAGVSRVINEAF
                     SDLGLAGWLAVVLVYMVMAIQYESLLYPLIVMFTMPLAGIGAIGFLVLTGQSISITSI
                     IGLIVLAGIVVNNGIVLVDFINQLKAEGMATREAVREAGRARLRPILMTALTTILGLV
                     PLAIGHGAGSELQQPMAITVIGGLTTATFLTLFVIPIVYELMDELAQRLSRKGGTVIE
                     G"
     gene            complement(6143..6886)
                     /locus_tag="PLU15_09865"
     CDS             complement(6143..6886)
                     /locus_tag="PLU15_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31258.1"
                     /translation="MEDVVPSGGSVKKRCALWGRLIMVCLLGVALIMCNMASANAAPS
                     GEEIAAKVTEKIKGVAALQADIASEFSDPSSKSPTRMTIRVSGDKGSGITRMEITQHP
                     VFEGQIIIVDVKKDLTTVYMPVTGQAYRGKSATMATQLGFDVGAFDLEKLLSLDLSAM
                     VKCSYLRQEKVDRLPHYVLETRVVQSPQEYQIVWVDCETYTIKRVEAYNSQGKRVATI
                     IINNLKLDTKIDPNKLRELPKGTRITEIK"
     gene            complement(6961..7719)
                     /locus_tag="PLU15_09870"
     CDS             complement(6961..7719)
                     /locus_tag="PLU15_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOO31259.1"
                     /translation="MGKKNHLPKQYHTKIWRAIYEFGLIEPDDRILVGLSGGKDSLFL
                     TYALKIIQSYSAIPFTFEAATVDIGFSDLFDTARIEEQCASLGVGCHIIGTHIADIIE
                     ERREDENPCAVCSYFRRAALNRYASEHGFNKVALAHHQDDAMETFLMNILYSGRVYAL
                     PWKTYLSRTGITVIRPMMYLSEKDVVKGFKRTGLEVVEAFCPYSGNTTRAKVKQLIVD
                     LAKENRKVRPNLAAAMRDGDDVELWPPIVRHNRS"
     gene            complement(7797..7887)
                     /locus_tag="PLU15_09875"
     tRNA            complement(7797..7887)
                     /locus_tag="PLU15_09875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7851..7853),aa:Ser,seq:gct)
     gene            complement(7941..8029)
                     /locus_tag="PLU15_09880"
     tRNA            complement(7941..8029)
                     /locus_tag="PLU15_09880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7993..7995),aa:Ser,seq:tga)
BASE COUNT         2020 a         2488 c         1873 g         1763 t
ORIGIN      
        1 tagaattaac cctcacattg accgccccat ctctcaaatc atcgatcaaa tgcgcatctg
       61 tttccaatgg ccgctcctaa agcgcagagc ggctattgtg ccccgagcca cccagtcaat
      121 agccattgca taccaagcgc ctgcaaggcc ccagccaaat acattcacaa acagccatgc
      181 gaggcagaaa cgcactcccc aaacgcacgc catagtgaaa atcagaacag ctctcgtatc
      241 tccagccccg cgaagagcgc ccacaaagac ataggaagtg gccattggca cttgagctac
      301 ggccatgata cgcaggacct ctgcgcccat actgatgatt tccaggtcgt ccccgtacag
      361 cctcatcagg gcgtcgggaa agcagaatag gaccacgccc atgagaccca ttatagtcgc
      421 gctcatcttc catgactcga tgcctgcccg ttcagccaca tcgggcctat ccgccccaag
      481 cgcctggcca accattgtgg tggctgccac cgcaaaggca aaaccgggca tgtaagaaat
      541 cccctcggca ttaatagcaa tcgcgtgcgc tgcgtaggcc gcagtgccca gagcagagac
      601 tatcctgaca tagaaggata ttcccagact caagctgaca cgttcaaggg aggccggcaa
      661 acccacggca aagaccctgc ggataagcac cgggtcgaac cgaaaacgct caccctgagt
      721 gcgcacagag attctgcctt ttcccgaaaa cagatgccaa agaagcattg cagttccgat
      781 cgttcgtgcg attgtagtag acaaagctgc acctgtgacg ccaagagcag gggcgccgaa
      841 cttcccgtat atgagtatgt aattccccac aatattggca agattgagga caatattgac
      901 cctcatgggg taccgcgtat caccggcgcc tcgaagggca gatgaagcta tcatatttat
      961 gacaagcatg aatagccccg gcatgagaac gcgcatgtag ctttcggcat aagggaccac
     1021 atcagggcct gccttcataa agcgaacaaa gttgggagtg aaatagtagc cgaacacggc
     1081 aaagaccgct gcaagaatgc tcccgatagc caacgattga gcggtaacgc ggtcagcgcg
     1141 gccctcgtct cccgctccca taaaccgcgc cactagggcc gtagtgccga ctccgatgcc
     1201 cataaaacac ccctgaatta tgttaaacgg ctgatttgaa agacctacag cggcaacgca
     1261 ttgggaaccc agcctgccaa ccatgatcat gtccgccatc tgggtaaccg tctgcagaac
     1321 aagctcggtc actacgggcc acgcaagggc caagatcgat ttgcgcatag cagatggatg
     1381 cgacatcatc tcgtctaagt cgactccgga aaatatcccc tgggatgcgc cccccgattc
     1441 ctgaaccgaa tcatccttca tctcgcatga cccaacttcg ctcactgcgc ccctatcaca
     1501 cccgttccta ttgccgctat cccatgccag gtcatctacg gcaggtcccc cgcaacatga
     1561 tatatctaaa ctaatggcta cgtcgtcgct ttctgatctc tccgtcatgc ttagaatccc
     1621 ccgtctgtaa ttcttgaccc cgggaccccg aagagcaact ctgcctcgcc ggtcccaggg
     1681 ccacactaca cgtttgtaat tcggcggcca tgattttgca tcgccaagat gaaacggaat
     1741 cgagctgccc actggcaagc caggcatccc attgattgcc agccttgtca gcctctagct
     1801 cagaatctcg atgcaagctc caggccgatc gccttggttc gcagatcgta tctcgcggtc
     1861 acactggaga tgatgccctc gttaaaatcc aagcctacag cgaaagccgc ccagtctatc
     1921 ggctcaaaaa gctctggccc gtcgtactca tgctcactca tgatcggagc gcaaatcctc
     1981 agtttcagat cagcccgatc gccaagcgaa agccaacccg tcccggaaac ccaagcatat
     2041 tcggagcctc ctccgccctg tacaacaagg tcgatctccg cccctgaact gtccgtcacg
     2101 tcggccctga atgtcagctt cgacaggagc atcactcgag gttcgccaaa agccagaccg
     2161 gagtagaatt ttatcgcaac gccaagatcc tccgggctgt atctcgcaat gtacccgata
     2221 tctgttcgcc aatcggattc gccaagctcc ctcatatatc caattttcca ggagtcagct
     2281 ctcgttcgcg agctttgaag ggctccaacc acgtctatcc agcccctacc tgtcagatcc
     2341 cttccaggct catcactcca gtcactctgc ccgattagac gagcgggctg ctcgtcaaat
     2401 ccgctgggca ccacgccgaa attcgttctc aatcctattc gagccgtgga ccccgttgcc
     2461 tggccgacca acgccatcac tggcccatcc ccacaatcct gaagatatgc cataaccgag
     2521 ccttcggctg cagccataag gctaaaaacc ctagccatct cggctcccac gccaaaggtg
     2581 taggcggccg atccttcact aaatgcaggc aaactcggac agatagcccc gcctattcgc
     2641 agtgtagcgc cttcccaatc aaggtcggac gcaattccaa gccagtttag ctcgagattg
     2701 agcgggccgt cttcagaatg cataactgac aaccccccga agaaaacagg ggtcccctct
     2761 acaacagtga ttgaagccgc gttcgccacc gaagagctaa acaaaaatac agtaacaagc
     2821 agcgcagcga cggtccactt cttgctatcc ttcaattacc gtgccccctt tgcggctcag
     2881 cctctgggca agctcatcca ttagctcgta aacgatcggg atcacaaata gcgtcagaaa
     2941 tgtggcggtt gtaaggccgc caatcaccgt tatcgccatc ggctgctgaa gctcagaacc
     3001 tgccccgtga ccgatggcca gcggcacaag accgaggatc gtggtgagcg ccgtcatgag
     3061 gatcggcctc agacgcgccc tgcccgcctc gcgaaccgct tcgcgtgtcg ccattccctc
     3121 cgctttgagc tggttgatga agtcaaccaa cacaatgccg ttgttcacca ctatgccagc
     3181 aagtactatc aacccaatga tcgaggttat actgatgctc tggcccgtga ggacaaggaa
     3241 tccaatggcc cctatacccg ccaaaggcat cgtaaacatg actatcaagg gataaagcag
     3301 agactcatat tgtatagcca tcaccatgta aacaaggacc actgcgagcc agcctgcgag
     3361 gcccaaatcg gagaatgcct cgtttatgac acggctcaca cccgcaagtt caacagaaca
     3421 gccgtccggc gcctccactt cgtgggctgc agacagagcc aagcttcccg cctgtgatag
     3481 cttcattcct tcaaacgcga tttgcacaag ggcataacgc cttccattgc gcctgcttat
     3541 cgtcatcggg ccatcaatta tctcaggcgt cacaaccttg cccaccagaa cctcgttgga
     3601 tggcgccagc attgttgata tagcactcga actggttcgg gctgctgctg aaacgttgct
     3661 tcccagcccg ctcgcgccct caagcaaact tgctgagctc aacgaagacg taatcggcat
     3721 gttcaaaata ctgtcaagat caggcactcc gtcagatgcc ggcctcacaa ttacgggaat
     3781 gagctcccca tttcgctcca catatgtcac cgtagatccg aacatcccgg tgcgaacccc
     3841 gagcccaacc tggcccacgg taagccctcc catgagcgcc ctcgtacggt ttacatcgag
     3901 caagataaca ggctgggttt cctttgtcga cgaagcaact tcaacgaatc cgggttcttt
     3961 acgcagcctt tcggcgattc tggccgcgta atccgagatc tcctttggat ctgtcccggc
     4021 tatctccaac gttatactat tgctcaggta atctgaactg ccacccgtca aagcgccata
     4081 tcccgaagtg tccactgaat actcgagatc agggtagtcc aacttgatgt cgctgaggat
     4141 ctccctaatc tcggccccag cctctgcaac cgaggtcatc gtttctcctt cacgtaagag
     4201 ctttaccgcc accgacgctt ggttagcgct tccgccgcta gccatggcca caaaatcccc
     4261 gctgccggaa gaacccactt cagcagtgac attctcgatc cccggaatct tcatcaattc
     4321 gttctcgact cttagcgaga cctcgttagt aacctctacc ggagtccctg gaggaagatg
     4381 aagtccgaca tcgactcttc ccatatccat tgaccctaag aattcggtcc caagcctggg
     4441 aaaagcgaag agcgccacaa cgagggcgat cccaatagcg cccaacaccc accatctctt
     4501 cgcaagcgcc caatcgagca tgcgatcgta tattccaccc aaagactcct tgcgcttcct
     4561 atcccgtttc gcgcgttcaa tataggaatc ggacacgcgc acgaacgaac ttgccatcaa
     4621 gggcaagacc gtaagggcta ctatgagcga cgccaacaaa gaaaaggtca ccgtcatggc
     4681 gagatctctg aaaatgtgcc ctgccatgct ctggatgaac acaacaggaa gaaagaccgc
     4741 aatcgtggtc aaagtagaag ccgtaatagc tctcgaaacc tctgctgtgc cttgaatcgc
     4801 ggcctcgcgc ggcgactctc ccagttcttg ccgccgaaag atattctcca aaacaacgat
     4861 ggagttatct actaacattc caacacccag agccagtccg cccaaggtga ggaggtttac
     4921 cgtgagatca aaggcgtaca tcatcacgaa cgcgaatatc atcgaaagcg gaatggagat
     4981 cgctaccaag agcgtgctcc cgaagtgtcg caaaaacaga aacaaaatcg cgatagcgag
     5041 aaccgcccca agaccggcgt tttcagcaag gctgtctata gagctcgtaa taaacgtgga
     5101 ctggtcgaac acatagccta tctcgatggc ccgatgctcc tgcccgagct gggctaatgc
     5161 ctccctcacc tgcctggcca ctgtcaccgt attctcgccc gactgcttgt agatcatcat
     5221 tatgacagca ggcttcccat tcacacgcgt atatccttca gccttttcaa acgtttcctc
     5281 aactttggcg acatcgccga ccgtgaggaa ggttggcatg aagaaagaca gacccaagaa
     5341 ccctccgaga ccatcggggc tttcagttgg ctttttcagt ccaatggcga gatcggcaat
     5401 atcgtcggca gatgtaaact tagcccccac cctggtctga tagcgtaccc catcgtctat
     5461 taccacgccg gccgggattg acaagttctg atatgttatg agttgctgca gcagaagcgg
     5521 gctgagccca ctttccacca atttctcatg gtcgtattca acgcttatca cccggtcagc
     5581 cccaccgctt atagagactc cggcaacccc gccaacgcgc tctacggcag gcttgaccac
     5641 ttctctagta gcttgggtga catcgcccac atcacccggc atgccaacag ttaccatcat
     5701 catggggatc tgagacggat cgagcagagt aacgatggga ctctgcgttc cttcaggcaa
     5761 ttcatgcttc atcgtatcaa tcttgacccg catgtcttcc tgggcggcgc caagatcgct
     5821 cccccagtta aactcgcaga agcaaaatga tacgttctcc atgctagtgg atgtaacatc
     5881 ctttatgttc cttacagacc ttagcgcccg ctcgattgga gcggtcacca ggctttcaac
     5941 ggtctcagga tcagcattgg ggtaaaccgt aactaccgcg gccatcggga gatttatgtc
     6001 gggcagaaga tcgagaccga tcctgtccag agagagaacg ccaaatagaa tgcatgccgc
     6061 tactaggata cccaccgtta ctggtcgatt gactgaacgt tctacaatat tcactagact
     6121 accccccata ggtcccggat cgctacttta tttcagtaat ccgggttccc ttggggagtt
     6181 cccggagctt gttcggatca atttttgtgt cgagtttgag gttattgatg attatcgttg
     6241 caaccctttt cccctgagaa ttgtaagcct ctaccctttt gatcgtgtat gtctcgcagt
     6301 caacccagac aatttgatat tcttgagggc tctgtaccac cctcgtctcc aacacatagt
     6361 gtggaagcct gtccaccttt tcctgacgca ggtagctgca ctttaccatg gctgacagat
     6421 ctaatgagag aagcttttca agatcgaagg cacctacatc gaaccccaac tgggtggcca
     6481 tagtagccga tttgccccgg tatgcctgcc ctgtgacggg catgtatacg gtcgtcaaat
     6541 ccttcttcac gtcaacaatg atgatctgcc cttcgaagac tggatgctgg gtgatctcca
     6601 tccgagtgat gccgctgccc ttgtcgcccg atactcggat cgtcattctt gtgggagact
     6661 tactggaagg gtcagaaaac tctgacgcga tatccgcttg aagcgcggca actcccttga
     6721 tcttctctgt cacttttgca gcgatctctt cgcctgaagg agcagcattg gccgacgcca
     6781 tattgcacat aataagcgcg acgccgagca ggcaaaccat aataagccta ccccataaag
     6841 cgcaccgctt cttcacactt cccccactag gcacaacatc ttccacgata ctgcccccca
     6901 tccctgcaaa tagacgacca cacttattat aggcatctca gaaaacccgt gtcaacaaag
     6961 ctagctcctg ttatgacgca caattggcgg ccaaagttcc acatcatcgc catcgcgcat
     7021 agcggccgcc aaattcggac ggacttttcg attttccttc gcaagatcga cgatgagctg
     7081 ttttaccttg gcacgtgtcg tgttcccgga atacggacag aaagcctcga caacttcgag
     7141 tccggtacgc ttaaaccctt tgactacatc cttttcggaa agatacatca taggccgtat
     7201 gacggttatc cctgtgcggg acaggtatgt tttccatgga agggcataca cgcgcccaga
     7261 gtagaggatg ttcatcagaa acgtctccat cgcatcgtct tgatgatgag ccaatgccac
     7321 cttattgaag ccatgttccg acgcgtagcg attgagagcg gccctgcgaa aataagaaca
     7381 tactgcacat ggattctcgt cctcccggcg ttcctctatt atgtcggcaa tatgggtacc
     7441 gatgatatgg cagcccacac ccaacgatgc gcattgttcc tcaatgcgtg cggtgtcaaa
     7501 caagtcggag aaacctatat ctaccgtggc ggcctcaaat gtgaagggaa tggcggagta
     7561 agactgaatt atcttcaaag catatgtaag gaacaagcta tccttgccgc ctgaaaggcc
     7621 cacaaggatt cgatcatccg gctcaatcag tccgaattcg tagatggccc gccagatctt
     7681 tgtgtggtat tgctttggaa gatgattctt tttgcccatg tctcgccccc ccagcgtcca
     7741 aagaaaaggg gcatgacctc ctattccgag ccggtcatgc ctcataagaa taaaactggc
     7801 ggagagaggg ggattcgaac ccccgctagg gtttccccta ctaatggttt agcaaaccat
     7861 ccccttcagc cacttgggta tctctccgca caggctgata catgagtcaa aaacaactat
     7921 ccatcgcgat ttgcgaatta tggcggaggg ggtgggattc gaacccacgg cccatttctg
     7981 agacaccggt tttcaagacc ggctccataa accgctcgga cacccctccc atcaagtgcc
     8041 tccatagtat atcacccatg tcgacgccca gtcaagacac tgtccgcctg agccattttt
     8101 gatgtaacgg aagcccacga taacgcgggt ttgaaaggcg gcta
//
LOCUS       DAOSYL010000082         7921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7794_length_7921_cov_7.512586, whole genome shotgun sequence.
ACCESSION   DAOSYL010000082 DAOSYL010000000
VERSION     DAOSYL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_7921_cov_7.512586"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..932)
                     /gene="pcp"
                     /locus_tag="PLU15_09885"
     CDS             complement(309..932)
                     /gene="pcp"
                     /locus_tag="PLU15_09885"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HOO31260.1"
                     /translation="MARILITGFDAFGGEEVNPASRAVQHLGASGVWKQGGVEVITKE
                     IPTVYGASARAVQNAICELSPQAVISVGQAGGSTGIRVERVALNLDDARIPDNSGNQP
                     SDRLIEPEGPVAYWATIPVRGVVEAVRAKGIPAFLSYSAGTFVCNHVFYSTCHFVASN
                     NLPIKVGFMHVPYLPEQAVGKTETPSMSEECIVAALEAAVDAVAASL"
     gene            complement(1040..3151)
                     /locus_tag="PLU15_09890"
     CDS             complement(1040..3151)
                     /locus_tag="PLU15_09890"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOO31261.1"
                     /translation="MTKEYSLGNRHFELYIDGERGCTSRLVNRFTGDDYLKSCTAAGP
                     MYKLYCIDKETGEKVEVLPESAVNIEEGVAGDRSSLAISFDSGRIAGAAEAPANIGAT
                     VNIDAADDDPESLWTIEIRNEDERYKVVEVLFPYFRGFYLGETWEDDVIIYPHHAGER
                     TLAPVREYTTERYLGFGRGATTRGKDGVFSREINYCGLASMDWMYYYDDRNGFYLASY
                     DKDFLVTGLRVETGGPDDPWMGWGLRKYVVVKQGETWRSNPSAVAITTKDWHWGARRY
                     RKWIDGIIEMPDNPEYLKDETILNQCYNFKRNGVIFNRFSDIPSMYDAGRLDYGMRHM
                     FIASWNRSGFDQDYPEYQPDMELGTPWQLAEGCRYVNENEGFVTFYINSRIFHVKSDF
                     FDTLGKKWAMKDHAGEMYHEVYGPHEFVVLCPSNKEWQDYLIEMGSWMVRSYGAKGIY
                     LDQLGSAEPFPCYDPDHTHSDIGRFNQGYLRVLRELLARIRSINEDSFLMIENCGDIY
                     GSYVWGSLTWNGEDYDEFYNLFKYTFPEYVQVNMCNPKRHLEGEARARRLHKDLNRAL
                     LLGSVFWVGLEKTATLEDELHAYLKAAITLRNRLNPFFKRGTFVDDEGIVEKPEGMEV
                     VHWRLDGGGDLYIISNPERVEGGVLATELPEGGIEGVRYFDIDGNEGAIEYRRGSRGA
                     AEIAEISLPKGAGEVLCIIAK"
     gene            complement(3156..3899)
                     /locus_tag="PLU15_09895"
     CDS             complement(3156..3899)
                     /locus_tag="PLU15_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOO31262.1"
                     /translation="MLTSEYFANMKAVLERIESTQMEAIEKSAEAITESLLSDGAWHI
                     MDTGHMLMYEAIGRTGGLMAVRPVRVSLNVENPVRYRERGVSKKRVFMDSIEGLPQFI
                     VEKSNMLPGDVLLVGSVSGINILPVQMAMDAKKLGLTVIGLTAVEYSSNLEAKHSSGK
                     RLFEVCDIVLDNCSNVGDTLVYVDELEQGICPSSGIAASYIIWAVQARVVEMMLERGK
                     KPHIYMSNNMPGAGEFNAKAWAEYEKVGY"
     gene            complement(4006..5271)
                     /locus_tag="PLU15_09900"
     CDS             complement(4006..5271)
                     /locus_tag="PLU15_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO31263.1"
                     /translation="MKSSFRRALLSAAVVVVMLSMCVPMEAKSNRTKVQVISWWDFTS
                     SEPLKQLKAKFEELNPDLELEYLQIGSGYADKMLVMIAGGADLPDVMMLAMDKIPVYA
                     SKGAIMNLDKYIQKDYKDEMKQLFPVVADAVTYNGSFYAMPRDVTSKVMFFNTKLFKE
                     AGVAIPDEDWTWADFEAIARKMTRDIDGDGKPDQWGFYFRKYMDGFTHWLMQNGGGLC
                     TKEGKSLIGKPETIEALKFLQKLAVDGVMPTETQARQFGNAQTAPFIAGRVAMVEGGL
                     STTVDLKNAGVDYVIRPLPKGKQRVTTAFVNAWAIPSGAKRPDLSWRVLKFFASKEAQ
                     EIALMSGMGLPARKDTNISAFVKIRPDHKYLIESLSFSKPFPSPVYGVDFFKMVEQEF
                     DLMWLGQRSVEDAVKAVEKQAADILSGKF"
     gene            complement(5417..6259)
                     /locus_tag="PLU15_09905"
     CDS             complement(5417..6259)
                     /locus_tag="PLU15_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31264.1"
                     /translation="MKRKKRISTVILYIVLIFLAVIMIYPFVWSVTASFKTNQQIYGG
                     NPLDLIPKPFTLNNYKRVLELLPFGRFLLNSMFLATVVPAISIILASMAAYAFARLEF
                     KGRDFIFMLLLGVMMLPGHVTLIPRYALMIKLGWVNRYIALIIPAIFSASLVFKIFFM
                     RQHFLSIPRELEEAALIDGCSQWGVWWNIMMPNSKPALAALAIIGFKTEWNSFLWPNV
                     VINDFMKMPIQVGLTYLQSNAQSSWDILMAGTTIAIVPVILIFICFQQYFMAGSLTSG
                     MGGR"
     gene            complement(6280..7224)
                     /locus_tag="PLU15_09910"
     CDS             complement(6280..7224)
                     /locus_tag="PLU15_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31265.1"
                     /translation="MGKQADSGLATTSGSPKPSNKRRAALKRELTAWSFVLPSLIGLG
                     IFVYGSLVYSLYVSFTSWDLLTPPKWVGLANYARTFKDPNFIQCMGNTLYFVVAIVPL
                     GIILAMAMAIALNKERPGFKFFRGAYYMPSITSTVAIGMVWLWIFNPDQGVLNTILQF
                     FGVANPPRWLESVKWAKPALSIMRIWQVSGYYMIMYLTGLQNIPKDLYEAAEIDGATK
                     WQQIKSITIPLLANTTVFATIMLTIEAFNLFEAIYVMTEGRPGGTTNTILYYIYTQAF
                     ERYRMGYASAMAWILFAILFVVTILQFNARRKKEEIGY"
     gene            complement(7423..>7921)
                     /locus_tag="PLU15_09915"
     CDS             complement(7423..>7921)
                     /locus_tag="PLU15_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31266.1"
                     /translation="LRAVALASEGRCEATVLMDMAMEMFGVERARDIIKAGLTRERIA
                     GFAMAVSSAAAQGDDVACRILADAGADLGRLGGHIITGLGMKGQGCPVALSGGASKAG
                     APLRDSFAATIKRIDPSCKIADPKLSPAGGALLIAYDLAGIELDWERSNRILACAEGF
                     DELRP"
BASE COUNT         1838 a         2334 c         1862 g         1887 t
ORIGIN      
        1 gaaggaaata gttatatgaa aggaaccaac ccagagaata cgcaagtttt tcgtacacac
       61 caagcaaagc aagagcgtgt ggacgccagg tcgtactcga agatgtgatg attgcacttt
      121 gagtcagcgt cgccctaggc taaccattat ctcgtgtgca caaaccgtct ccacggcaat
      181 gaaaaactca ataccgaaca ttcgcgttgc agaacacccc caaccacaaa gccatttcat
      241 cacgaatcca ccacatcacc agtctgctca acgccccgag ccggccattt gccagcaaag
      301 ggcagagatc acagactcgc cgccaccgcg tcaactgccg cctccaatgc agccacaatg
      361 cattcctccg acatgctcgg agtctccgtc tttccaactg cctgttcagg gaggtatgga
      421 acgtgcatga agcccacctt gatcggcaga ttgtttgagg cgacgaaatg gcaggtcgag
      481 tagaagacgt gattgcacac gaaggttcca gcgctgtatg aaaggaatgc cggaatccct
      541 ttggcccgta cagcctctac aactcctcgc acgggaatag tggcccagta agcaactggc
      601 ccctccggct caataagcct gtcgctgggc tgattccctg aattgtctgg aatcctggca
      661 tcatcaaggt taagggccac cctctcaacg cgtatcccgg tcgagccgcc cgcctggcct
      721 acgcttataa ccgcctgggg cgaaagctcg catattgcat tctgcactgc cctagctgaa
      781 gccccataca ccgtaggtat ttcctttgtt atcacctcaa cgccgccttg cttccacact
      841 ccagacgccc caagatgttg aaccgccctg gaggcgggat tcacctcctc gcctccaaaa
      901 gcgtcaaacc ctgttataag aatcctggcc atagctaagc cccctcaaag gcgtccattc
      961 tagccggtgc agactccctt ttagcctcaa tgaaccacgc cgccacggga ggccacccga
     1021 acactgcccc ggcatgcacc tactttgcga tgatgcacag cacctcgcct gcccctttgg
     1081 gcaaagatat ctctgcaatc tcggcagccc cccggctccc ccgcctatac tcaattgcgc
     1141 cttcgttgcc gtctatatcg aagtatcgca cgccttcaat cccgccctcc ggcagctccg
     1201 ttgccagcac gccgccttcc actcgctcag ggttcgagat gatgtacaga tcgccacccc
     1261 cgtcaagacg ccaatgaacc acttccatgc cttcgggctt ctcgactatt ccttcatcat
     1321 caacgaatgt cccccttttg aagaaggggt tgagcctgtt cctcaaggtg atcgcggcct
     1381 tcaggtacgc gtgcagctca tcttcgagcg tggcagtctt ctcgaggccc acccagaata
     1441 ctgagcccaa aagcagcgcc ctgttgagat ccttgtggag cctacgcgct ctggcctcgc
     1501 cttccagatg gcgcttgggg ttgcacatgt tcacttgaac atactctgga aacgtatact
     1561 tgaacaggtt gtaaaactcg tcataatcct cgccgttcca agtgaggctt ccccagacgt
     1621 acgacccgta tatgtctccg cagttctcga tcatcaaaaa tgagtcttca ttgatgctgc
     1681 ggattcgggc aagaagctcc ctgagaactc tgagatagcc ctggttgaat cggccaatgt
     1741 cgctatgggt gtgatcagga tcgtagcatg gaaacggctc tgccgaccca agctggtcaa
     1801 ggtagatgcc cttggcaccg tagcttcgaa ccatccagct tcccatctct ataaggtaat
     1861 cctgccactc cttgttcgaa gggcaaagca ccacgaactc gtggggaccg tatacctcgt
     1921 ggtacatctc gcctgcatgg tccttcatcg cccatttctt gcccaaagta tcgaagaagt
     1981 cggacttgac atggaagatt cgcgagttga tgtagaatgt tacaaatccc tcgttctcat
     2041 tgacataccg acacccttca gccagctgcc atggcgtgcc gagctccatg tcgggttggt
     2101 attccgggta atcctggtcg aatccgctcc ggttccatga agcgatgaac atgtgccgca
     2161 tgccataatc tagtcggccc gcatcataca tcgatggtat atcgctaaag cggttgaaga
     2221 taacaccatt tctcttgaaa ttgtagcact ggttcagtat cgtctcatcc ttcagatact
     2281 ctggattgtc gggcatttcg atgatgccgt cgatccactt tctgtacctt cgcgcacccc
     2341 agtgccagtc cttggtggta atggccacag cagacggatt cgatcgccac gtctcgccct
     2401 gcttcaccac gacgtatttc cggaggcccc atcccatcca cggatcatcc ggcccgcccg
     2461 tctcgactct cagtcctgta acgaggaagt ccttgtcata tgaagccaga tagaaaccat
     2521 tgcggtcgtc ataatagtac atccagtcca ttgacgccag accgcagtag ttgatttccc
     2581 tgctgaacac cccgtccttc ccgcgagtgg tagcccccct cccgaaaccg aggtagcgct
     2641 cggtcgtata ctctctcaca ggcgcaagtg ttcgctcgcc tgcgtggtga gggtatatga
     2701 ttacatcatc ttcccatgtc tctcccaagt agaacccgcg gaaatagggg aataggactt
     2761 caaccacctt gtaacgttcg tcctcgttgc ggatctcaat cgtccacagg ctctcaggat
     2821 catcgtctgc ggcatcaatg ttcacagttg ccccgatatt tgcaggtgcc tcggctgccc
     2881 ctgcaatgcg gccactatcg aaagaaatcg caagcgatga tctatccccc gcgacgccct
     2941 cctcgatgtt caccgcgctt tccggcaaaa cctcaacttt ctcaccggtc tctttgtcaa
     3001 tgcagtatag cttgtacatg ggacccgcag cagtacaaga cttcaagtaa tcatcaccag
     3061 taaaacgatt cacgagccga cttgtgcagc cccgctcgcc atctatgtag agctcgaaat
     3121 gcctgttgcc cagagaatac tccttagtca cctcatcagt aacctacctt ctcatattct
     3181 gcccatgcct tggcattgaa ctccccggcg ccgggcatgt tgttgctcat gtatatgtgc
     3241 ggcttcttgc cccgctcgag catcatctcg actacgcgcg cctgaaccgc ccagatgatg
     3301 tatgaggcgg cgatgcccga cgacgggcag atgccttgct cgagctcatc cacgtagacc
     3361 agagtatcgc ccacattcga acaattgtcg agcacgatgt cgcacacttc aaatagtctt
     3421 ttcccactgg aatgcttggc ctcaaggttg ctggaatatt cgacagccgt gagcccgatt
     3481 acggtcaacc ccagtttctt cgcatccata gccatctgaa caggcaggat gttgatgccc
     3541 gagactgacc ctacaagcag cacatcgccg ggcagcatat tgcttttctc gactatgaac
     3601 tggggcagcc cttctatgga gtccatgaag acccgctttt ttgagacccc ccgctctctg
     3661 tagcggacgg ggttttcaac gttgagagag actctcaccg gtcggaccgc cataaggccg
     3721 cctgtccttc caatcgcctc gtacatgagc atgtgccccg tgtccatgat atgccacgcc
     3781 ccgtctgaaa gcagggattc agtaatggcc tcggcacttt tctcgatcgc ctccatctgc
     3841 gtggactcga tccgttcaag cacagccttc atattggcaa aatactctga tgtaagcata
     3901 cacacgcacc tcacgatact tgctaggtta aacgtcgcgg gcattcctaa cgcgacaggg
     3961 cgcacctgtt gcagggcgcc ccgttacgca tctattggat caaaactaga acttgcctga
     4021 caggatatcc gccgcctgct tctcaacggc cttcacggca tcttccacag acctctgacc
     4081 caaccacatg aggtcgaatt cctgctccac cattttgaag aagtcgacgc catacactgg
     4141 cgatgggaac ggtttgctga aggagaggga ctcgatcagg tatttgtgat caggacggat
     4201 cttcacaaaa gcgctgatat ttgtatcctt ccttgcggga aggcccatgc cgctcatgag
     4261 ggctatctcc tgcgcttcct tgctggcgaa gaacttcagc acgcgccagg agagatccgg
     4321 cctcttcgct cccgacggga tggcccaggc attcacgaat gcagtggtta cgcgctgctt
     4381 gcccttgggc agcggtctga tcacgtaatc aaccccggcg tttttcaggt cgactgtggt
     4441 agacagtccg ccttcaacca ttgcaactcg gcccgcgatg aacggtgccg tctgagcatt
     4501 tccgaactgg cgagcttgtg tttctgtggg catcacgccg tcaaccgcca acttctgcag
     4561 gaacttcaga gcctcaatgg tttcaggctt ccctatgagg gacttgcctt ccttggtgca
     4621 aaggcctccg ccgttctgca taagccaatg ggtaaaccca tccatgtact ttctgaagta
     4681 gaatccccac tgatccggct ttccgtcgcc atcgatgtcg cgggtcatct ttctagctat
     4741 agcctcgaag tcggcccacg tccagtcctc atccggtatc gcaactcctg cttccttgaa
     4801 gagcttcgtg ttgaagaaca tgaccttgct cgtgacgtct ctcggcatgg catagaagct
     4861 gccattatac gtaacggcat cggccacgac cgggaagagc tgcttcatct cgtctttata
     4921 gtccttctga atgtacttgt cgaggttcat gatcgcgccc ttggatgcgt agactgggat
     4981 cttgtccatg gcaagcatca tgacgtcagg aaggtcggcg ccgcctgcaa tcatgacaag
     5041 catcttatcg gcgtatccgc ttcctatctg tagatactcg agttccagat cggggttgag
     5101 ttcttcgaac ttcgccttga gctgcttaag cggctcgcta gatgtgaagt cccaccagga
     5161 tataacctga accttggtcc tgttcgactt tgcttccata ggtacacaca ttgaaagcat
     5221 gactaccaca actgctgctg aaagcaacgc ccttctgaaa gaagatttca tcttttcact
     5281 tcctcctttt tgcttgcgga aaggtagtac tcacacggtt cagcagtgat cagatcatcg
     5341 acgcttcgta gccggtctac cgccccctta agttgatgca ttaccatgac agcctttgtc
     5401 caaacctcga caatccctat cgtcccccca ttccggatgt gagcgatcca gccatgaaat
     5461 actgctggaa acagatgaat atgaggatga cggggacaat agctattgta gtgccagcca
     5521 tgagaatatc ccagcttgac tgagcattgc tctgcaaata tgtcaggccg acctgtattg
     5581 gcattttcat aaaatcgttt attaccacat taggccagag gaagctgttc cattcagtct
     5641 tgaatcctat gatcgccagg gccgccagag ccggtttgga attgggcatc ataatgttcc
     5701 accagacgcc ccattgcgag caaccatcaa tcaaggcagc ctcctcgagt tcccggggaa
     5761 tgctcagaaa atgctggcgc ataaagaaga tcttaaacac caagcttgcg ctgaatattg
     5821 cagggattat cagggctata tacctgttga cccatcccaa tttaatcatc aaggcatatc
     5881 ttggaatcaa cgttacgtgt cccggaagca tcatcactcc cagcagcaac atgaagatga
     5941 aatccctgcc cttaaactcc aagcgggcaa aggcatatgc cgccatcgac gccaggatta
     6001 tcgagatcgc aggaactact gtggctagaa acatcgagtt gagaaggaac cggccgaacg
     6061 gaagcagttc aagaaccctc ttgtagttgt tgagggtgaa cggttttgga atcaggtcga
     6121 gcgggttccc tccgtatatc tgctgattgg tcttaaacga cgctgtcact gaccagacaa
     6181 agggatatat cattatcaca gccaggaaga taagtactat atacaaaatc acagtggaga
     6241 ttctcttctt gcgcttcatg ttctttcccc ctccccggcc tagtagccga tctcctcttt
     6301 tttgcgacgg gcattaaact gaaggatcgt taccacaaac aagatggcaa agagtatcca
     6361 cgccatagcc gacgcatagc ccatcctgta tctctcgaat gcttgggtat agatgtagta
     6421 gaggatcgtg ttggtggttc cgccgggacg cccttcagtc attacataga tcgcttcaaa
     6481 gaggttgaag gcttcgatcg tcaacatgat ggtggcgaat actgtggtat ttgcgagaag
     6541 aggaatcgtg atgctcttta tctgctgcca tttggtggcc ccgtctattt ctgccgcttc
     6601 atagaggtcc tttgggatgt tctgaaggcc cgtgagatac atgatcatgt agtaacccga
     6661 cacctgccaa atgcgcatga tgcttaaggc gggtttcgcc catttgacac tttccagcca
     6721 gcgaggggga ttagcaaccc caaagaactg taggatagtg ttgagcactc cctggtccgg
     6781 gttgaatatc cacagccaca ccattccgat agcgacggta gacgtgatcg atggcatata
     6841 gtaggcccct ctgaagaact tgaaccctgg ccgctcctta ttgagcgcta tagccatagc
     6901 cattgccagg attatcccca aaggaacgat ggctacaaca aaatagaggg tgtttcccat
     6961 gcattggatg aaattgggat ctttgaacgt tcgcgcgtaa ttagccagtc caacccattt
     7021 gggaggggtg agaagatccc agcttgtaaa ggatacgtac agtgaataga caagtgagcc
     7081 gtagacgaat atgccgaggc ctatcagtga tggcaacaca aaagaccatg cggtaagctc
     7141 gcgtttgagg gcagcgcgtc tcttgttgct aggcttcgga cttccggaag tagtggccag
     7201 tccactatca gcttgctttc ccatgtagcc aacctccatt aagaagagtg cacggtaagt
     7261 atcacaactc gctactgacc atatataagc tacagcatct ccgggataac ctcggatttt
     7321 gccccaaggc ttaatgctgt gttattcgca agccaacatt cctagttata tttattatac
     7381 tctttctatc tataactcaa gatcccttct tcggatccac gtttacggac ggagctcgtc
     7441 gaacccctca gcgcacgcca ggatgcggtt cgatctctcc caatccagtt caattcctgc
     7501 caaatcatac gctatgagca acgcgccacc tgcaggtgaa agcttcggat cggctatctt
     7561 gcagctcgga tcgatccgct ttatcgtagc cgcaaacgaa tcgcgcaagg gcgctccagc
     7621 cttggatgca ccccctgaca gggcgaccgg acacccctga cccttcatgc ccaaaccggt
     7681 gattatgtgt cccccaagcc ttcccaaatc ggctccggca tcagccagta tcctgcaggc
     7741 tacgtcatct ccctgggccg ccgcggacga aacagccatc gcaaatcccg ctattcgctc
     7801 ccgagtcagt ccagctttga taatgtccct ggcacgctcg acaccgaaca tctccatggc
     7861 catatccatg agcacagtag cttcacatct gccttccgaa gcaagggcaa cagcccgaag
     7921 t
//
LOCUS       DAOSYL010000083         7826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7933_length_7826_cov_5.899241, whole genome shotgun sequence.
ACCESSION   DAOSYL010000083 DAOSYL010000000
VERSION     DAOSYL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7933_length_7826_cov_5.899241"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2667)
                     /locus_tag="PLU15_09920"
     CDS             complement(4..2667)
                     /locus_tag="PLU15_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31267.1"
                     /translation="MSHVAGDCNARVDALMNALDIIEAVAERTGKDEERRHVRQLKKD
                     LHSVAKNGNSLLTRHAEATAELARIVMPKPRFIVDGCHAGILFGDVGYEELDDPRMAM
                     EKLFALLDLALSHDAGFSLDWGMSTLEEVSKLLPDDIERLRAVIESTDRIEIVNGSYS
                     QPFPMTIGWESNIRQFEEGLRCADAILGVRPRTYVAQEFGHHPQMPQILKGYGFDGCA
                     LRVRLNGEVPSCEDPIVWWEGDDGTAILCLTTLESVPTCDECAGLFYRSLASGFASLE
                     MSDADFGAFNNLEDVVYPVLDRTEAIICPNRDDILGKFSTYSEVIDAVRDMPIRRAGF
                     ASASFRPSVCQNAQLFGAIKAAEAILIVTEILDVYAHILGIELELDQIISDSWRMLLA
                     AENHDSFVVPDHIPGTYTVERSAIFGSYHGLRSNKTVGEKGLELAKDATHACTVAVDR
                     LKVAVGAEINRGIGGSIDAFDLSEQMWHSLALLDVSGVKGKKSTIGRLDFPLGEMTNF
                     RIEFPGTEAEWQVVDEDRWDDGSLRSADVAVQADIPALGWLVARVSPEDEASETAQND
                     AIASITDGAAVIDTGLIGAKVTLDPILRVDLQDGSSGEELACIDITRPGSGGDCKVVA
                     TGPVMGEIRVDGGENNEFGTVSCSAWKNEASVRVECTQAAQHEYVIRPNHRINRVLAD
                     YPFGIGEVTPGVFHALNVVVIETEETNLCIVHAGDPYFEYELEENLVRYAPRCGPSSI
                     RVFVLHDCDDPLEISRSCMLKPLMVQPPHISSSSDRATKTCGEGDGKAFAGRLIDIRG
                     DVLITSLRRTQIDEVEIRLFNSGREDRQFSLGRLPGYGAWQETRLDGMAVKSGCRPCL
                     CGSDGWVLPPGRIATFRCRRRSSSPDVAKQM"
     gene            complement(2701..3549)
                     /locus_tag="PLU15_09925"
     CDS             complement(2701..3549)
                     /locus_tag="PLU15_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO31268.1"
                     /translation="MSSGVRTRANGGKVLVYILLIIGVIISLFPFYVSLVHALQPDYY
                     IESSGAPDLWPLEFRFENFAIAWEKGNFSRAMLNTVFIAAATTAVKILGSCMAGYAFA
                     KRDFKGKNVIFAILMLTIMIPDEVTMVPTYMLMLKLGWVNTYLPLIVPAVGDVFSIFL
                     FKQFMESVPNSLIDAARIDGCNEWQLFWKIVLPTAKPAIATMAILGFQASYNDFMGPL
                     IYLNDERLFTVQLQLKAFDRFYNSHFDQVLGSAANLIACVPVIVVFVVFQKYFVESVS
                     LSGMKG"
     gene            complement(3542..4453)
                     /locus_tag="PLU15_09930"
     CDS             complement(3542..4453)
                     /locus_tag="PLU15_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO31269.1"
                     /translation="MQTIHENKRKRSKGLSANAWSYVFIAPAMIGFFVFRAFPIGFAV
                     WVSLTDWELIGPPPSFVGVQNYVQAFHDPVFGKAFANTLAFTLMTALGAALVGLIFAA
                     LLDRNIRGSKFFRTSLYVPVITPVVAVVRIWTWLYSPSPGGLFNWVAGLFGSGPYRWI
                     MDPDMALPSLALMTIWQGFGWDMVIILAGLKGIPNVFYEAATVDGASWWDKFAHITLP
                     LLRPVMLFVFIMGGINGFKTFAQPYLMQGPMNSTLTTSLYIFQNAFDNFRMGYAAALS
                     FIVSVVIMLLSVVQFRAFGGEEGISYE"
     gene            complement(4773..5873)
                     /locus_tag="PLU15_09935"
     CDS             complement(4773..5873)
                     /locus_tag="PLU15_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO31270.1"
                     /translation="MDRGSSVPLYIQLKELLRDKIESGDLGDGELLPTEQELCEQFNL
                     SRYTVRQALGELVKEGYIERVRGLGSFVRRVPKVANAPAVGIIVPYTDDFYTSSIIRG
                     VEGVARSEGHNLIFANSNNHPEQEEILLRKLRNDGVRGLIIFPCEDSVYSVAVEGLMS
                     IGFPVVLVDREYKTLDCPAVVSDNVDGGEKAVDYLVSLGHKRIAFAMTIFNHTSSVQD
                     RLLGYRTALARHHIPWDKSLLLTYAPRLYDAPEDDADHIAPLRRYLAREDRPTAIFAV
                     NDLVALDILRAAAEVGLDVPGDLSVVGFDDIPVVSELPTPLTTVAQPHLEIGAVGAKM
                     LFHIIAGRTLERTRVVLPVKLVVRQSCSELSQ"
     gene            6230..7495
                     /locus_tag="PLU15_09940"
     CDS             6230..7495
                     /locus_tag="PLU15_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOO31271.1"
                     /translation="MKNRRRFLVTVSLLIACSILAVQPLATIAAGFETFKLPIAEFEP
                     GMPSLYLRGPVEPAKNVILLISDGTGINHRTLVRIASGGANSKLPSERLFEVGLANTH
                     SLDSLVTDSAASATQIATGNWVPNRTISVNADGEPLQTILELAKTLGKSTGIITTDRV
                     TGATPAGFAGHVPDRDMKNEIAQIILENDSVDVLIGGGKKNFQSPVAGKKGDRTDGRD
                     LLEEAAERGVTICNSIYELRADDSPRTWALLSSGSMTAAVKRNYTLGDLTSEALRRLS
                     QNPEGFFLMVEGDQIDGGGHDNDIWRMALELLDFETAVEVATEFAENRDDTLVIVVSD
                     HETGGLAITNGQAQSTEVEYGWIHGGHTGTPVAIYSSGPNSFLLGGHIYLADISKIIA
                     FGWDIYDFGPDRAVEGPGAKWLLSKQNQQ"
BASE COUNT         2005 a         2179 c         1835 g         1807 t
ORIGIN      
        1 caatcacatc tgctttgcca catccggact ggatgagcgc cttctgcatc tgaacgtggc
       61 aatccgccca ggaggcaaca cccagccgtc actcccgcac aaacaaggcc ggcagccgga
      121 ttttactgcc ataccatcca acctcgtttc ctgccaggcg ccataccccg gaagtcttcc
      181 gaggctaaac tgccggtcct ctctgccgga gttgaagagc ctgatctcta cctcatcgat
      241 ctgcgtccgc ctcagtgagg tgatgagtac gtcgcctcga atgtcgatga gtctcccggc
      301 gaaggccttg ccgtctccct ctccacaagt ttttgttgct ctatcagagc tgctactaat
      361 gtggggaggt tgcaccatta atggtttgag catgcaggat cttgaaatct ccaaggggtc
      421 atcgcaatca tgaagcacaa acacccgtat actggatggt ccgcaccttg gggcatacct
      481 gaccaggttt tcttcaagtt catactcgaa ataggggtct ccagcatgca caatacagag
      541 atttgtttcc tcggtttcga tcacaaccac attcagggca tggaacacac ccggcgtaac
      601 ctcccctatt ccaaaggggt aatctgcaag cacccggttt attctgtggt tcgggcgtat
      661 gacgtactcg tgctgtgccg cctgggtaca ctcgacacgc actgaagctt cattcttcca
      721 agcagagcac gatacggtcc cgaattcgtt gttttctcct ccatcaaccc gaatctcccc
      781 catcacgggg ccagtagcaa ctaccttgca atcgccgcca ctgcccggcc tagtgatatc
      841 tatgcaagcc aattcctcac ccgaagaccc atcttgcaga tcaactctga ggattggatc
      901 caatgtcacc tttgcgccta ttaggccggt atcgatgaca gcagcgccgt ccgtgatgct
      961 tgcgatcgcg tcattttggg cagtctcaga agcctcatct tccgggctaa ccctggcaac
     1021 gagccagccc agcgccggaa tgtcggcttg cacagcaaca tcagcactcc gaagacttcc
     1081 atcgtcccat ctatcttcat caactacctg ccattccgct tcagttccag gaaactctat
     1141 gcggaaattc gtcatctctc cgagggggaa atccagcctc cctattgtcg actttttccc
     1201 cttcactccg ctcacatcca atagcgccaa gctatgccac atttgttcac tcagatcgaa
     1261 ggcatcgata ctacctccaa tccctcggtt gatctctgcg cctacggcta ccttcaggcg
     1321 atcaacagca acggtgcatg catgggttgc atcctttgcc agctccaagc ccttttcgcc
     1381 aaccgtcttg ttggatctca gtccatggta tgacccgaat atagctgacc tttccacagt
     1441 gtaagttcca ggtatatggt cgggcaccac aaacgaatca tggttttcgg ccgcgagcag
     1501 cattctccat gaatcactga tgatctggtc tagctctaac tctatgccga gtatgtgggc
     1561 gtacacgtcc aggatctcgg tgacaatgag gatcgcctca gcggccttta tcgcgccaaa
     1621 gagctgtgca ttttggcaga ctgatgggcg gaacgatgcg cttgcgaaac cagcacggcg
     1681 aataggcatg tctcttacgg catcgatgac ctctgagtag gtgctgaatt tccccaatat
     1741 gtcgtctcta ttagggcaaa tgatcgcctc tgtccgatcg aggacagggt atacaacgtc
     1801 ttccaggttg ttgaaggctc cgaaatctgc atcgctcatc tccagcgacg caaacccaga
     1861 agcaaggctc ctgtagaaca gtcccgcgca ttcgtcgcaa gtaggaactg actcaagtgt
     1921 agtcaagcag agtattgcgg ttccatcatc accctcccac cagactattg ggtcttcgca
     1981 cgatggaact tcgccattta gcctgacccg caacgcgcac ccgtcaaacc catatccttt
     2041 gagaatctga ggcatctggg gatgatgacc gaactcctgc gcaacatatg ttctgggcct
     2101 tacgcctaaa atcgcgtcgg cacatctcag tccctcttca aattgacgaa tattcgactc
     2161 ccaccctata gtcatcggga acggctgcga gtaggagccg ttcactatct caatcctatc
     2221 agtagactct attactgccc gaagcctctc tatatcgtca ggcaagagct tcgaaacctc
     2281 ttccaaggtg gacatgcccc agtccagcga gaaccctgca tcatggctga gcgcgagatc
     2341 tagcagcgca aagagctttt ccatcgccat acggggatca tcgagttctt cgtatccgac
     2401 gtctccaaaa aggatgcctg cgtgacaccc atccacgata aacctgggct taggcatcac
     2461 gatcctagca agctccgcag tagcttcggc atgcctagtg agtaagctgt tcccgttttt
     2521 ggccacactg tgcaaatcct ttttcaactg ccgaacatgc cggcgctcct catcctttcc
     2581 tgtgcgctca gcaacggcct ctattatgtc tagcgcgttc attagcgcat caacgcgtgc
     2641 attacaatca cctgcaacat gactcataaa caaacccccg actccaagtt tggcatggca
     2701 ttaccctttc atccctgaca atgacacgct ctccacgaag tatttttgga acacaacaaa
     2761 gaccactatt acgggaacac acgcgatcag gttggcagca ctgcctagaa cctgatcgaa
     2821 atggctgttg taaaacctat caaaagcctt gagctgtagc tgaacggtaa acagcctctc
     2881 atcgttgaga tagatcaacg gacccatgaa gtcattgtat gaggcctgaa aaccgagtat
     2941 agccatggtc gcaattgcag gtttggctgt gggcaatact attttccaga acagttgcca
     3001 ttcgttgcaa ccatcaatgc gtgctgcatc gataaggcta ttcggaacag actccatgaa
     3061 ctgcttgaac aggaagatgg aaaaaacatc tcccactgcc ggtacaatca gcggcaagta
     3121 ggtattaacc caacccaatt tgagcatgag catgtaggtt ggcaccattg tcacttcatc
     3181 cggaatcata atggttagca tcaatatagc aaatatcaca tttttgccct tgaaatcgcg
     3241 tttcgcaaac gcgtacccag ccatgcatga gccaagtatt ttcaccgccg tagtcgcggc
     3301 ggcgatgaac actgtattca acatcgctct ggagaagttg cctttttccc acgcgatggc
     3361 gaaattctcg aatctaaact cgagcggcca aagatccggg gcgcccgagg attctatata
     3421 gtaatcaggc tgcaatgcat gcaccaagct tacatagaaa ggaaataggc tgataatgac
     3481 ccctatgata aggagtatgt atactaacac cttccctcca ttggctcgtg ttcgcactcc
     3541 actactcata gcttatcccc tcctcgcccc caaaggccct aaactgcacg actgatagca
     3601 gcatgataac gaccgaaacg atgaacgaca aagcggcggc atatcccatc ctgaaattgt
     3661 cgaaggcgtt ctggaaaatg tacagtgatg tggttagcgt ggaattcatg ggcccttgca
     3721 tcagatatgg ttgagcaaaa gtcttgaatc cgttaatgcc gcccatgatg aagacaaaca
     3781 gcataacggg cctgagcaag ggaagagtga tgtgcgcaaa tttatcccac caactggcgc
     3841 cgtcaacagt tgccgcctcg taaaagacat tgggtatacc cttgagccct gcaaggatta
     3901 tcaccatgtc ccagccaaat ccctgccaaa tagtcatcaa tgcaaggctt ggaagggcca
     3961 tgtcgggatc cattatccat ctgtacggac ccgaaccaaa caggcctgca acccagttaa
     4021 acagtccccc cggactcggg ctatacagcc aggtccagat cctgactact gcaacaacag
     4081 gcgtgattac ggggacataa agggaggttc tgaaaaactt tgagcccctg atgttcctgt
     4141 cgagcaatgc cgcgaatatg agtccgacta gagcggctcc taatgccgtc atgagggtga
     4201 atgcaagcgt attcgcgaag gccttgccga acactgggtc atgaaacgcc tgcacatagt
     4261 tttgaacccc aacaaaactt ggaggaggcc cgatcagttc ccagtcagta aggctcaccc
     4321 acacagcaaa gccgatcgga aaagccctga agacaaagaa tccaatcatg gccggagcaa
     4381 tgaaaacata cgaccaagca ttggctgaaa gccctttgga tcgcttccgt ttattttcgt
     4441 gtatcgtctg catcacgggt cccctttctc cagatatgcg accacagcat ctccagcgcc
     4501 ggcacactaa cagcccaacc gaccatatgt ccggacaatt atgggcgcaa aagggctaca
     4561 gtgtctcgga cgccacctgc agatagtctt taatggccag cataataggg tgtgtctcat
     4621 gattagacca tccaagagtg ctaatcgggc tacaccccat actgccagca gttgttgttt
     4681 gtacgtacat cgtcacaccc tattctacat cgcctggcgt aatcctgctt catgcacgaa
     4741 atcgcacatc ttcctaagat agatgctatg cgttactgac tcagctcact gcatgattgg
     4801 cgcacaacaa gcttcacggg caagaccact cttgttcgct cgagggtgcg gccagcaatt
     4861 atgtggaaaa gcatcttggc gccaacagcg ccaatttcca gatgcggttg ggcgacggtt
     4921 gtgagcgggg tgggcagctc ggatacgacc ggaatatcat cgaacccgac caccgaaaga
     4981 tcgcccggaa catcgaggcc cacttcagca gcagcgcgca gaatgtccag cgctacaagg
     5041 tcgttgactg caaaaatggc tgtaggtctg tcctctcttg caaggtatct acgcaaaggg
     5101 gcaatatggt cggcatcgtc ttcaggcgca tcatatagcc tcggcgcgta cgtcaacagc
     5161 aacgatttgt cccacggtat gtggtgccgg gccaaggccg ttctgtaacc taagagtcgg
     5221 tcctgtactg aactggtgtg attaaatatt gtcatggcaa aagcgatcct tttgtggccc
     5281 aatgatacca ggtaatccac cgccttttcg ccgccatcaa cgttatccga cacaacagca
     5341 ggacaatcca gagtcttgta ttccctgtcg accagaacca cgggaaatcc gatagacatc
     5401 agaccttcta cagcaacact atacacgctg tcttcgcagg ggaaaatgat caacccccgc
     5461 accccgtcat tccgcaattt ccgtaggagt atctcttctt gttcaggatg gttgtttgaa
     5521 ttagcaaaga tgaggttatg cccctcagat ctggcaaccc cttcaacccc tcttatgatg
     5581 ctggatgtgt aaaagtcatc ggtgtaaggt acaataatcc ctaccgccgg cgcatttgcc
     5641 actttgggaa cacgcctcac aaatgaccca agtcctctaa ccctttcaat gtaaccttct
     5701 tttacaagct ctcccaaagc ctgtctcaca gtgtatctgc tcaggttgaa ttgctcgcac
     5761 agctcctgct ctgtgggcag cagctcacca tcgcctaagt ctcccgattc tatcttgtcg
     5821 cgcagaagct cctttagctg gatgtaaagc ggaaccgaac tgcctctatc cattatttgc
     5881 attgctccct cacacggatt gtacggacat aatgtgttta tcatattcta ctcattacgg
     5941 ctgtatcctg caaacgagca ccatttagga ttcgagctag actcgtatca cggcctttgg
     6001 accggcaacg ccaaagtcgc tcaaagcttg cgattatgcg ttgcgttcac aaggaggatt
     6061 cataacacag gcggcgaatc aatgagtcgc agcacatgta cggacaaata ggggaggcca
     6121 atcgcccttg tttaagccat aatggcccga agctttcgaa ttcctcgcca gcacttgctg
     6181 gttctgcagg cattcgcaag gaaaactcta ttctcgggag atgatcattt tgaagaatcg
     6241 aagacgattt ctggtcacag taagtctact gatagcatgc tcaatcttgg ccgttcaacc
     6301 tctggctact atagcagcag ggtttgaaac cttcaaactt cccatcgcag aattcgagcc
     6361 tggcatgcca agtctttacc tgcgtggccc tgtcgaacct gccaagaatg taatactgct
     6421 catctcagac ggtacaggaa tcaatcatcg cactctagtt cggatagcgt ccggtggcgc
     6481 aaattccaag ctgcccagtg agaggttgtt cgaagtaggc cttgcaaaca ctcattcact
     6541 cgactctctt gtaactgatt ctgcggcatc agccacccag attgccacag gcaactgggt
     6601 tcctaacagg actatcagtg taaacgctga tggcgagcca ctgcagacca ttctcgaact
     6661 cgcaaaaact ctaggaaaat ccaccggcat aatcacaaca gatagagtta cgggagcaac
     6721 accagccgga tttgctggcc acgttcctga tagggatatg aaaaacgaga tcgcccagat
     6781 catactggaa aacgatagcg tggatgtgtt gataggcgga ggaaagaaga attttcagtc
     6841 cccagttgca ggcaagaagg gcgatcggac agatggccgg gatctgctcg aggaagcggc
     6901 agaaagagga gtaactatat gcaacagcat ttacgagctc agagcggatg actctccgcg
     6961 gacatgggcc ttgctcagca gcggatcaat gacggcggct gtaaagcgaa actacaccct
     7021 cggcgatctt acatccgaag ccttaagacg cctgtcccaa aatcccgagg gtttcttcct
     7081 gatggtagag ggagaccaga tagacggcgg tggacacgac aatgatatat ggcgaatggc
     7141 tctggaactc ttggattttg aaacagctgt tgaggttgcc acggagtttg ccgagaacag
     7201 agacgacaca ttggtgatcg tagtgtcgga tcatgaaaca ggaggcctag ctatcaccaa
     7261 tggtcaagcg caaagcactg aggtggaata tggctggatt catgggggcc acacaggaac
     7321 tcctgtggcc atctacagct ctggacctaa ctcgtttctg ctcgggggac acatatacct
     7381 tgccgacata tcaaagataa tcgcttttgg atgggatata tacgatttcg gcccggatag
     7441 ggctgtggag ggaccagggg ccaaatggct tctgtcgaag cagaatcagc agtaaatccc
     7501 tagaccatgg ataaatgcga tctccagctg cggggagggc aaatccctcc ccgcttttct
     7561 tgttctccag tccccatcat acttcgccgc gtaccagtct tctcgactca acagcttagt
     7621 gtggatatga gcgccagatt ctgcagaacc aaaatccggc cggaaataaa cacgccctga
     7681 attaaaaacc atagcacaag caaggttgtt ggttcggacg aagacaacac acaagcctgt
     7741 cagcagtgct aacccgaaaa ggctgtgcga atttccgaga atgtgaaagg caaaaaaccg
     7801 agctgagagt gcagccgagc atgaca
//
LOCUS       DAOSYL010000084         7816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_7949_length_7816_cov_7.012112, whole genome shotgun sequence.
ACCESSION   DAOSYL010000084 DAOSYL010000000
VERSION     DAOSYL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_7816_cov_7.012112"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..569
                     /locus_tag="PLU15_09945"
     CDS             324..569
                     /locus_tag="PLU15_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31272.1"
                     /translation="MLVRLSEIRLLVLNKWKAAPPNSIESCDILEIIDSSVWLKTHIE
                     YGGKQEILLCVAPRSYSINRGLAGTTGFFAPERVDGF"
     gene            complement(599..1444)
                     /locus_tag="PLU15_09950"
     CDS             complement(599..1444)
                     /locus_tag="PLU15_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO31273.1"
                     /translation="MKIAVVTDSASDLPNEVREAGQIHVVPIRVLFGEDQFRDQVDIT
                     PEQFFDKLNTSGVLPSTSQPSPADFINLYEILLKKHDAVISLHISEKLSGTYQSACIA
                     RDAVSPSRIEVIDTRAASMAQGFIAMEALQAAAAGSSREHVLHAVQKAMDTTRLAFTV
                     DSLEHLMRNGRIGKAASMFGSLLSVKPIIALEDGELTPMAKVRGSKRVLERVMECIRK
                     DVGNGAIKVAVIHTNTHDRAREWFTAVQGAFVCKEAYLVQCGAVIGTHVGPTGLGVAW
                     MPADQ"
     gene            1770..2003
                     /locus_tag="PLU15_09955"
     CDS             1770..2003
                     /locus_tag="PLU15_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31274.1"
                     /translation="MAITDKGALCVVNIEPLQKSYSNEREVTHVLRDITLYVCAGERF
                     SVMGPLGSGMRLPAGFSAPRLGWMRFNYEPFWG"
     gene            2321..2848
                     /locus_tag="PLU15_09960"
     CDS             2321..2848
                     /locus_tag="PLU15_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOO31275.1"
                     /translation="MKSGNLQRQVQLAILTAVALVLHMIESVLPPLPVPGAKIGLANV
                     ATLVCLRHMGFWPGVAVSAVRSILGSMISGKFWGLGFWMSFVGATASAVAMGISLQVV
                     ASGTSLVWTSVVGGVVHASCQLIIAVAAMRQPPLFAGAPVLLGLSIITAVSIGLVCDN
                     LDAPVRRLIQANKRR"
     gene            2856..3212
                     /locus_tag="PLU15_09965"
     CDS             2856..3212
                     /locus_tag="PLU15_09965"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOO31276.1"
                     /translation="MRRRALPILLVLVFGVALVAFGMYEQKKAPEGSVVVVKSIGEDY
                     NRIVARQPLSSDEKVIIPVEGPLGTTVVEVVGGRAHVVSSPCPDKICVRMGWLKRVGD
                     YSACLPNRVLVEVVDE"
     gene            4120..4347
                     /locus_tag="PLU15_09970"
     CDS             4120..4347
                     /locus_tag="PLU15_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31277.1"
                     /translation="MGKDCTVVDAIIDTVETKVDFGEDFKAGSDLTAECKTKRGTYGM
                     KKASVIKKEWHRQIVEIEKWMAGKTIADIRL"
     gene            4605..5705
                     /locus_tag="PLU15_09975"
     CDS             4605..5705
                     /locus_tag="PLU15_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOO31278.1"
                     /translation="MTKTASKWMALILSGVLLAFAVVTCQKAKNRYTKYTDTFFDAFD
                     TLVTVVVYVQSEEEFDSYYQQIRARFLELHRLYDKYNEYEGINNIKTINDNAGIRPVQ
                     VEQEIIDLILFSKDWCARTGGKTNIALGSVLEIWHEYREQGLYDPASAKLPPMEDLLE
                     AAQHIDIDKVIVDAENGTVFLGDSEMSLDVGAVAKGYATEVVAREMMAAGLKSGMISA
                     GGNVRAIGKPLDGVRECWGVGIQNPVESIVADDGVLDVVFVNDASVVSSGDYQRYYVV
                     DGTVYHHLIDPATLMPARYYHAVTVVTEDSGVADFLSTAVFLLPYDQSRSLVENLDGV
                     EALWVMPDGKVEATSGMKRIMRSHGASGTKAM"
     misc_binding    6581..6845
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6937..>7816
                     /locus_tag="PLU15_09980"
     CDS             6937..>7816
                     /locus_tag="PLU15_09980"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOO31279.1"
                     /translation="MANVRFFTDQHLPLEMHKVRIVQKLNLPPIERRLEAITEAGNNT
                     FLLQNRDIFLDMLTDSGVNAMSDQQLAAMMIADDSYAGSETFTKLENKVIELFGMPHF
                     LPAHQGRACEHIIASTLVKPGNVVPMNFHFTTSKAHIVRCGGIVRELLIDEGLKPTSE
                     FPFKGNVDLNKLRQVIEEEGAGNIPFVRLEAGTNLIGGQPLSLENVREVTAVCHSNGI
                     PVVLDASLLQDNLYFIHEREEACKDMSIREITRALADEVDVIYFSARKLGFAKGGGIC
                     IRDRVMFEKMRELVPLF"
BASE COUNT         1796 a         1766 c         2271 g         1983 t
ORIGIN      
        1 ggagacatta acgaggttgg gatagggaaa aggacggatt caggcggcaa cggaggaaat
       61 ccgtcatgct gcagcttcta tggtatgaat acaagagttc tgcagcccaa atggcagact
      121 gcgtgcaaga atggtagttg ttggaacctt gttgctcatc ttgctgcctg tgattggatt
      181 aaaagctcac agaacgtgct aatcagtggg tgtatgggcg caggctatta ttgtcttgcc
      241 tgcgcccata cacccactct gctgatacta catagagtgc tgcgcctcat atccgaactg
      301 gccatcgccc gcggagaccg atcatgctgg tcagactctc cgagatacgt ttgcttgtcc
      361 tgaacaagtg gaaagcagcg cccccaaatt ccatcgaaag ctgtgatatc cttgagatca
      421 tcgactcgag cgtctggctg aaaactcaca tagaatacgg cggaaagcag gagatattgc
      481 tttgcgtcgc gcctcgctcc tactcgatca accgggggct tgcgggaacc actggattct
      541 ttgctccgga acgggtagac ggtttttagt ggcatacgca atggcggcac ttggccagtc
      601 attgatcagc tggcatccac gctactccca acccggttgg cccgacatga gttcctatta
      661 ctgctccgca ttgcacaagg taggcttcct tgcatacaaa agcgccctgg accgcggtaa
      721 accactccct ggcgcggtcg tgtgtgttgg tatggatcac tgccactttt atggcgccat
      781 ttcccacgtc cttgcgtatg cattccatca cgcgttctag aacccgcttg gatcccctta
      841 ctttggccat tggagtcaat tcgccgtctt ccagagctat gatgggtttg actgaaagta
      901 aggatccaaa catcgatgcg gctttcccta ttcgcccatt tcgcatcagg tgttctagcg
      961 agtcgaccgt aaaggctagt ctggtggtat ccatggcctt ttgaactgca tgaagcacgt
     1021 gttcgcggct tgagcctgct gctgcggctt gcagggcttc cattgcaatg aatccttgtg
     1081 ccatggatgc cgctctcgtg tcgatcactt cgattctgct tggactgaca gcgtctcggg
     1141 caatacaagc agattggtat gtgccggaaa gcttctctga gatgtggagc gaaatcactg
     1201 cgtcgtgctt tttgagcaag atttcatata ggttgatgaa atctgctgga gaaggctggg
     1261 atgtggaagg aagaaccccc gatgtattca gcttgtcaaa gaattgctcg ggtgtaatat
     1321 ctacctgatc ccggaattga tcctcgccga aaaggacgcg aattggtaca acatgaatct
     1381 gtccagcttc tcgaacttca tttggcaggt ctgatgcaga gtcagtcacc acggctatct
     1441 tcatttcgtc accctcaaac ggtattttcg gatgctgact gtatagggcg ccattcgaaa
     1501 agcggggaaa tcgggctgta ggggccggag gcgctgttag gctattgaag ctgtcggcat
     1561 ccaaaaagta ttttgtacat caaacaatat gataaacaaa tcatataatg ccgtttcgtg
     1621 tttgtcaatt agtttggaat accggagctt gagctgctgt agcgttggga ccagcagaac
     1681 agtcaattcc aaggtgtacc gctctcagac gactggggga ggaccgcctg tcccgtgcag
     1741 gcttgtagtc acttgctgac atactgacga tggccattac ggacaaaggg gcgctttgcg
     1801 tagtcaacat tgagcctcta cagaagagtt acagcaatga acgcgaggtt acacacgtgt
     1861 tgcgcgacat tactctgtac gtatgcgccg gggagcgttt ttccgtgatg ggcccgctgg
     1921 gctcgggcat gcgattgcca gcaggcttct ctgctccgag gcttgggtgg atgcggttca
     1981 actacgagcc tttttggggg tgagactcgg tcggcggtgc tttttctgct gtctgctttt
     2041 ccagtaggct gctcttttgg cgtctagcct gcagcaaaac tgccagactc aaccaggtag
     2101 aattccttgg aagtgagtgg cagggggaac aagtgaaaga cctggatgtc atgtctgtgc
     2161 catgccctag acggaggtta gcgctaggag tttgcccgtc gagtgtcatc gcaggcaaag
     2221 cgaatatggt atataatttg gattgtggct tagaaatgct tgtagcagcg tttttgttgg
     2281 ctttgatgtt gaactcgatg ccaatggcgg tgatttgcag ttgaagtcag gcaatctgca
     2341 gcgacaagtg cagctggcaa ttttgacagc tgtagctctt gtccttcaca tgatagagtc
     2401 cgtgttgccc ccgctgccag tgccgggagc gaagataggc cttgcgaatg ttgcgacatt
     2461 agtatgcctg cgccatatgg gattttggcc tggcgttgca gttagtgcag ttcgtagcat
     2521 acttggaagc atgatatccg gcaagttctg gggtttgggg ttctggatga gctttgtggg
     2581 cgcaactgcg agtgccgtgg ccatgggcat tagccttcaa gttgttgcca gtggaacatc
     2641 gctcgtttgg actagcgtcg tcggaggagt agtccatgcc agttgccagt tgatcattgc
     2701 tgtagccgcc atgagacagc ctccgctctt tgctggtgct ccggttttgc tagggctttc
     2761 gataatcact gccgtttcga tcggcttggt atgcgataat cttgatgcgc ctgtccggcg
     2821 tctgatacag gcaaacaaga gaaggtgatg catacatgag acgcagggca ttgcctatcc
     2881 tgcttgtttt ggtgttcggt gtagcgctag tggctttcgg aatgtatgag cagaaaaaag
     2941 cccccgaagg ctcggtggtg gttgtaaagt ccataggcga agattacaac aggatagtcg
     3001 cccgacagcc tctatcatcg gatgagaaag tgattatccc ggttgaagga ccgttgggca
     3061 ctaccgtagt agaggttgtt ggaggaaggg ctcatgtggt ctcgtcaccc tgccctgaca
     3121 agatatgtgt gcgaatgggc tggctgaaac gtgtaggcga ctacagcgca tgccttccca
     3181 atagggttct tgttgaagta gttgatgagt gagcttttgc atgggggatt gcgaggcttg
     3241 taatgattga gggactggga cagaggcagc aaggcgggag agctcgaact gggttgatcc
     3301 cgccttgcta tttctagtgg tcaggaccct ctgggcaata agtggctctt tgaacaccaa
     3361 gtgtaaataa atggctgcga ttgcgcgtgg ttcaggcgcc tcgatggcta ccattacagt
     3421 atctactgcc acgtgccgtt taggaatgta gcgaagtatg tagatgatct gccagttgcc
     3481 ttgttgggca gtgccgactg tgtagtggac ggcaaattcg agatgggtga ggcttgggga
     3541 gaaggagcct ttgttttgct cctaggctgc ggtatgcccc ggacgaatat gcaacattga
     3601 gctatcatgg ctgtcgacca cgagcatgcc atgtcacaag ttggaggtga atggcacggt
     3661 cctgtagcca aagccgtatg cgggaacgaa gcatggggtg ccggtctcct gtgggcctcg
     3721 cactttagcg cgcccgatat cgcggttacg atcgccggcc agaaagcctc ttgacttttg
     3781 ctagtacgtc tgtttgacgc ttggattggg ttgcaagcta actctgtgaa tgtgaatcct
     3841 gtcactatgg acgatgattt tgtgaagtga taagcttgga tagaatcaag ccctgcttca
     3901 gaaagggaaa tgaaaaaggc ggcattggtt cgggatgtaa ttcgtacaag ccttgttcca
     3961 ccggttgctt gatcttcaag cggttgggct gtctagaagc cgattgtata gtcgggtaca
     4021 tcacgtctat tgtgaaatcc aaggggcttt cggttatcgc taatggcaag ggtaattgct
     4081 ccggcagctt agatctgcac taccatcgcc taagcggtat tgggtaagga ctgcacagtt
     4141 gttgatgcga tcatcgatac agttgagaca aaagtcgatt ttggtgaaga tttcaaggca
     4201 ggctctgatc tgactgctga gtgcaagacc aagagaggaa catacggcat gaagaaggcc
     4261 tctgtcatca agaaagaatg gcacaggcag attgtggaga ttgagaagtg gatggccggc
     4321 aagactattg cagacatcag gctatgaagg ttaaaagcgg cgtgaggccg tttataccgg
     4381 attgctacgt gctcctgagc tcatttctca ctttgctgtg agtgtcgaag attacatcgc
     4441 tactgttgca aaggcctaca agagcgcaag ataagggtag gacagacttc ttgatccatt
     4501 tggctgatca cgcacctctc tcatctcaga tgggggaggt ttctctcagt cttttcgtgc
     4561 taaataggca gtgtactggt gaatataagt gagtggtggt gccgatgaca aagactgctt
     4621 caaagtggat ggcactgatt ttatcaggcg ttttgctggc atttgcggtt gtgacctgcc
     4681 agaaggccaa aaacagatat accaagtata ccgacacctt cttcgacgcc tttgatactc
     4741 tggtgacagt tgtggtttat gtccagagtg aagaggaatt cgattcgtac tatcaacaga
     4801 tccgcgcgag attccttgag cttcataggc tgtatgacaa gtataacgag tatgaaggga
     4861 tcaacaacat aaagacgata aatgataatg ctggaataag gcctgtccag gtagaacagg
     4921 agataatcga tcttatcctt ttttcgaagg attggtgcgc acgcacaggc gggaagacaa
     4981 atattgccct cggttcggtg ctcgagattt ggcacgaata tcgggaacaa ggcctgtatg
     5041 atccggccag cgcgaagctc ccgcccatgg aagatctgct cgaggctgcg cagcatattg
     5101 acatagacaa agtgatagta gatgcagaaa acggcacagt attcctgggc gatagtgaga
     5161 tgagcctgga tgtaggcgct gtagcgaagg gttacgcgac tgaggtagtt gccagagaga
     5221 tgatggctgc tgggctcaag tcgggcatga taagtgcagg cggaaacgtt cgggcgattg
     5281 ggaaaccact tgacggcgtt cgcgagtgct ggggcgtggg aatacagaat cccgttgagt
     5341 ccatcgtcgc cgatgacggc gtgctcgatg tggtattcgt caacgatgca tccgtagtga
     5401 gcagtggcga ctaccagagg tattatgttg tggacggaac ggtctatcac cacttgatag
     5461 acccggcgac gctgatgcct gcgcgatact atcatgcagt tacagtagta acggaggact
     5521 ctggggtagc ggatttcctg tccaccgcag tgtttctctt gccatacgat cagagcagat
     5581 cactcgtcga gaatctcgat ggggtagagg ctctgtgggt tatgcccgat gggaaagtgg
     5641 aagcaactag cggcatgaag aggataatga ggagccatgg cgcgtcgggg acaaaggcca
     5701 tgtagtattt gggcttttcg gcatgaaaca gttgatcatg ccggagaatc acaatgtgcc
     5761 acgtaatcct tcaattcctc gatggactca ccagtaggtg ttcgaagtcc gttttccgcc
     5821 agcaatgttt ggcagttctt ggtcctatca acctgcttca ggttctcgat ttgcactttc
     5881 tactctcgca atcaaaggga gacaatgcag atacttgggg cttcgtttga gttcaatctt
     5941 gcctaggtca aggagtatcg acagaactaa gaaagggact ggaatatgtt catcgaatga
     6001 ggcgccggca tgtgcggtga catgaatcct tttcttagcc ttggcgcaat cgctaatggt
     6061 gtgggagtgg aatcgggctc attgacggcg ctgatgcagc ggtgaaaatc gtctacaaac
     6121 ggatccgtgt cgcgggcaaa gttgggctag ccatgtgtac gcttcctctc ctggtgtgac
     6181 acgttagtat tacttgcata cgggctgagc ttttgatttt gtggtagtgc gtgaattctc
     6241 cggtcacagc ggggagcgca tcatgcttgc tgcacctgcc tgttcttggg gagcagactc
     6301 ctggcggttt tcctcctttc tttaatgcgt cggcgatgcg tgttgatttg ataggatgga
     6361 gcgattaatg cgggttggac tccgctgttg cctgcgaaag cctgtgttgg ttgctttgtg
     6421 tgaaggcatg cggagcgttg ctataccggg acataggctg ctgcacagga ccagtgtttt
     6481 gcaggaaagc atgccacttg aatttgttca cgaatggctg aaattgattc ttggcaaggc
     6541 gggtcggctt tgatataata acagccaata agcatacagc acaaagacga tgaccgagaa
     6601 gaagtaggac atgacgtagg cccgacagag agttcccggc tggtgtgagg ggcggggttg
     6661 ttatgtctga atacatcttg gtagtcgctc acccaaaggc gcattgacgc ccggtaggct
     6721 gagacggatt cacccgttac agtgatagag tatcgcgcac tattagcgcc gtacttgaaa
     6781 aggccggcaa cgcgagttgt cggcaaattg aggtggtacc acgggatttg gcgctcgtcc
     6841 tcttgctaag gcaggggacg ggcgcttctt gctttccccg caatatgggc gtgctggcgc
     6901 caaagggcac tcccaagtta aaggagtgta tttttgatgg caaacgtaag gttcttcaca
     6961 gatcagcact tgcccctgga gatgcacaag gtgcgtatcg tacagaagct gaatcttcct
     7021 cctatcgagc gcaggctaga ggctatcacc gaggcaggaa acaacacatt tcttcttcag
     7081 aatcgggaca tctttctgga catgctcaca gacagcgggg tcaatgccat gagcgatcag
     7141 cagttggcgg cgatgatgat agctgacgac agctatgcag gctctgaaac tttcaccaag
     7201 ttggaaaaca aggtgatcga gcttttcggc atgcctcact ttctccctgc ccatcaggga
     7261 cgtgcctgtg agcatattat tgcgtcaacc ttggttaagc ccggcaatgt cgtgccgatg
     7321 aacttccact ttactaccag caaagcgcat attgttagat gtggcggcat cgttcgcgag
     7381 ctactgatcg acgagggcct caagcctaca agcgagtttc cgttcaaggg aaacgttgac
     7441 ctgaacaagc tgaggcaggt aattgaggag gagggagcag gcaacatacc atttgttcgg
     7501 ctggaggcgg gcacaaactt gattggcggg cagcctctct cgttggagaa tgtccgcgag
     7561 gttacggcag tctgtcactc caacggcatc ccagtcgtac tcgatgccag ccttcttcag
     7621 gacaacttgt atttcatcca cgagcgtgag gaagcatgta aagatatgag catacgagag
     7681 atcacccgtg cactcgccga cgaggtggat gtcatctact tctccgcgcg caaactaggc
     7741 tttgcaaagg gcggagggat ctgtatccgc gaccgtgtca tgttcgagaa gatgcgggag
     7801 ttggtacccc tcttcg
//
LOCUS       DAOSYL010000085         7666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_8145_length_7666_cov_7.711339, whole genome shotgun sequence.
ACCESSION   DAOSYL010000085 DAOSYL010000000
VERSION     DAOSYL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8145_length_7666_cov_7.711339"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1071)
                     /locus_tag="PLU15_09985"
     CDS             complement(31..1071)
                     /locus_tag="PLU15_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31280.1"
                     /translation="MNRCAIASFTSSQTRQAHCGKSRAAIIAIIVLAIIPRCAIAAPQ
                     DIGPPAGMLEPVGPEEISDMLIQALQQRGCQARIEEDYLLMTFPDGQSHNLIFRDLRL
                     FLAYVAEEDWEEVIEVYVEIVFNSIEEIRSLVNSFQSLDDAICHLGVCLYPADYLVRF
                     PDEEPVYRRDIDGLITCLVWDFSDMSVTAEWKDIEKWDFDIDELFSTAMENTLAKYPV
                     APVEASVLPGLNLIIVESAGHPISAHMLAIDRLPGAMGPYGALVSAPAHNVVFIYPIA
                     DEGVMLALMAMAEIANDAVHVALNPLSDQVFWHNGQTFVNIPYIMGKEFPEIDIYNMP
                     PEFLMLLLTLSN"
     gene            1311..2870
                     /locus_tag="PLU15_09990"
     CDS             1311..2870
                     /locus_tag="PLU15_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31281.1"
                     /translation="MDGAYPYWGLAAAALGVYTLAMLGIGAWAGRQVKDAEDYIVAGR
                     KLGFPLLFGTVLATWICGGLFMGGMAESYKHGFQGAILDPVGPGLWLLVAPVFYCILM
                     RRARYMTVPDMISTRYSKHAGLICSLAIVVYELFWVCATVTAFGSILSTFLNWPLWVG
                     ILAGGLVLIVYTWQGGMWAVTVTDLVQMVLLCIGMIMMLGRVVDLAGGWHALIANAAT
                     KENALPFNVLPGSGGFYGWTGMWAWSYLISGVVAGLGANAAAQDYWERALSGRSIKTI
                     IWASATAGLVYLALGSIPYFIGIGAYEIAPSLSAEQVESLGPWLLVNYFSPAAATFIA
                     VAMAAAVMSSADSGLLALASVCGKNLLQLLNPKAGDGDVLKWTRRWVTIGGIISLVMA
                     LFFQEIFRVQIFGHAILAIAAFPMMTLGLFDRKSNNLGALTGTYLGMGVWIGFSLFLT
                     HVQGAGFWDASEIAMLPALIVSFATMFVVSRLTAAADPPKCLVDVDGNPLPTSGAVLS
                     CKALFRRGEIE"
     gene            complement(2969..4252)
                     /locus_tag="PLU15_09995"
     CDS             complement(2969..4252)
                     /locus_tag="PLU15_09995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO31282.1"
                     /translation="MTRTEQLKTRARRLLHEFKGDSYSHSLHQVGAFAAQFGKDVLVI
                     ANHSPWLAPILSTVTSSLEANDVSIAEGRIVPGSAPNSPVEDVYRIATYILHFKPDCL
                     VAVGGGSTIDAVKAANVTACLGPYEPELKAYLGVGLVSKAMSRTGAKLLPMIAVQTAS
                     GSGAHLTKYANVTYTLERQKKLIIDDAVTPDRAVFDYTVTKSAPQDVTLDGALDGLSH
                     CLEVFYGIGPESYELAQEIAEIGIELIVDSVPKVIDAPEADEPREALGLATDLGGYAI
                     MVGATNGGHLTSFSLVDITTHGRACAVMNPYYTVFFAPAIERQLRIVGDIYRRYGYIS
                     ADIDTLFGRDLGIAVAEGMIDFSRKAGFPITLGELPGFTDSHIDRALAAAKDPQLESK
                     LKSMPVKMDASSVDTYMKPILEAAKTGDLSLIVNM"
     gene            complement(4294..4917)
                     /locus_tag="PLU15_10000"
     CDS             complement(4294..4917)
                     /locus_tag="PLU15_10000"
                     /inference="COORDINATES: protein motif:HMM:NF016216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HOO31283.1"
                     /translation="MLGSKPRISAADTNLIFRHLKRRPAIPALKQAKELKLAAECDAK
                     LVFFLTGTIRDLRDVCNSAKDYGIAVFCHIDLIQGIGKDPAGIDWLADEIGISGILTT
                     RSNLIRAAKASGLIAIQRLFLLDSESLKTAIDIVSSSRPDAVEILPALVLPSLIHRLP
                     IRKLSPFIAGGLVETVPELEAVVSTGALGVSTSKQELWKFEKALPDR"
     gene            5207..6439
                     /locus_tag="PLU15_10005"
     CDS             5207..6439
                     /locus_tag="PLU15_10005"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOO31284.1"
                     /translation="MGRTVCPPDVKQRASGAKAVLVWAGLLALIFAALARPAGSLAAS
                     LDASGDVRIYLVDPSDAEPAFAIEHDGLELIAEFGSDENSLFVGAGSLRWGPGRSGSL
                     MMSGRAEPMPLVGYHLSRGNSDYVRFIGLMESDEARRLIGHRLEYTPTPDVVLGVSET
                     AVVSQNASRALYLPFPGFPLYALQRVVSQQDRAQDSLININLGADFSLFWDLMSSDFA
                     KWGGFAPRMELYGEIFIDDAQGHIPNRAYVPDMVGGLVGIDLPKLPWNLRFGANIEYV
                     AIANFVYSHRNPNNDYVYRGVMLGHPLGPDSDMLVLTLSYCPDKCAKLTLSGAVERHG
                     EGRAGQSWSHQHEPEDWFLSGVVETCARAVFGIERELAPMARLMGSLEYATKSNSGNV
                     LGQSSDEWNMRIGVGVKL"
     gene            complement(6484..6930)
                     /locus_tag="PLU15_10010"
     CDS             complement(6484..6930)
                     /locus_tag="PLU15_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31285.1"
                     /translation="MSYTGEEMNGRVLLLLTSATVILILTCASGSALCAPQLESLHAS
                     WIVVDGINLGCRMRITDTWRFALNFDMCHNSAYTTGSAICMYKNPIFVLTAGYVGAGI
                     SYELQSSRFYPNVLAGAEFGAAFAEYEWMPSPKNYGKLRCGMRVSF"
     gene            7040..7498
                     /locus_tag="PLU15_10015"
     CDS             7040..7498
                     /locus_tag="PLU15_10015"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOO31286.1"
                     /translation="MRRNCDMGGSRGSAWIWWALALAYPAFLLIASVRPEFGAEATRA
                     FIEKILPHLDPSQVHVVVVAFRKACHFLGYGFFAIVLANAIHTLRKGVRHRKWAVASC
                     IIAALIALGVAVLDEQIQSVVPHRTGASRDVALDFLGIVVGMLVKVGRGF"
BASE COUNT         1543 a         1854 c         2337 g         1932 t
ORIGIN      
        1 tgatcttggc gggtgcaggc atattgttgc ctagttgctc agggttaaca gcaacatgag
       61 aaattccgga ggcatgttgt atatgtcgat ctcgggaaat tcttttccca tgatataagg
      121 gatgttaaca aaggtttgcc cgttgtgcca gaaaacttga tcgctcaggg ggttcaaggc
      181 gacatggacg gcgtcattag ctatctcggc cattgccatg agggcgagca ttacgccctc
      241 atctgctatt gggtagatga agaccacgtt gtgggcgggt gcgctgacga gtgcgccgta
      301 aggccccatt gcgcccggca atctgtcgat ggcaagcatg tgggctgaaa tggggtgtcc
      361 cgcagactcc acaatgatga ggttgagccc gggaagcaca ctggcttcga caggggccac
      421 aggatatttt gcaagcgtgt tttccatggc ggtggagaag agctcgtcta tgtcgaagtc
      481 ccatttctcg atatctttcc attcggctgt aacgctcata tctgagaaat cccatacgag
      541 gcaagtgatc aggccatcga tatcacggcg gtatacgggc tcttcgtccg gaaaccgcac
      601 gagatagtct gctgggtaca ggcacacgcc taggtggcaa atggcgtcat cgaggctttg
      661 gaagctgttt accaggcttc tgatctcttc aatgctgttg aagacgatct ctacgtagac
      721 ctcgatgacc tcctcccaat cttcttcggc gacataagcg aggaaaagcc gcagatcccg
      781 gaagatcaag ttgtggcttt gcccatccgg gaaggtcatg agcaagtagt cctcttcaat
      841 gcgagcctgg cagcctcgct gttgtaatgc ttggatgagc atgtcagaga tttcttcagg
      901 gcccacaggc tcgagcattc cggcgggcgg gcctatgtcc tggggggcgg cgatggcgca
      961 acgggggatg attgccagga cgataatggc gatgatcgct gcccgggatt tgccgcagtg
     1021 cgcttgacgg gtctgagaag acgtgaatga ggcgatggcg caacggttca tttgagtccc
     1081 tcctataatc gggtgagtct cctttcttcg gtttgggcaa caactccttc ctacagtact
     1141 cctataaatt cgcgtcttca caacacgctg atttggtgct aatcttgatg ccgtctacgt
     1201 cggcagaatg gtccgaactt ctgcggtgca tctgattgtt tccgcttgga tgtggaagaa
     1261 gcaatccggc ttttttgggg gcgcgactat catgactgga ggaaacggca atggacggag
     1321 cttatccata ctggggtttg gctgcggcgg ctctcggggt ttacacgttg gcgatgctcg
     1381 ggataggggc gtgggcgggc aggcaagtga aggacgcgga ggattacatt gttgcgggaa
     1441 gaaaactcgg ttttccgctt ctgttcggca ccgtgctcgc cacctggatc tgcggcgggc
     1501 tgttcatggg aggaatggct gaatcctata agcacggatt ccagggggct atcctcgatc
     1561 cagtgggccc tgggttgtgg cttttggttg cccccgtctt ctactgcatt ctcatgcgca
     1621 gggccaggta catgactgtg cccgacatga tatctaccag atacagcaaa catgccgggc
     1681 tcatctgctc gctggcgatt gtagtatacg agctgttttg ggtctgcgcc actgtgactg
     1741 cgtttggctc gatcttgtcc acctttttaa actggccgct atgggtgggg atccttgctg
     1801 gcggattagt tctcattgtc tacacgtggc agggcgggat gtgggcggtt accgttactg
     1861 accttgtcca gatggtcctt ctttgcatag gcatgatcat gatgctgggg cgagtggtgg
     1921 atctggccgg gggctggcat gcgctcatcg ccaatgcggc aactaaggag aatgctttgc
     1981 ctttcaatgt cttgccgggg tcgggcgggt tctacgggtg gaccgggatg tgggcctggt
     2041 catacctgat ttccggcgtc gttgccggct tgggggcgaa tgcggctgct caggactatt
     2101 gggaacgggc tctttcagga cgatcaataa agacgattat ctgggctagc gcaacagcgg
     2161 gcctggtcta cctggcgcta ggttcgatcc catacttcat aggcatcggg gcgtacgaga
     2221 tagcgccttc gctcagcgct gagcaggtgg aatctttggg gccgtggctc cttgtgaact
     2281 acttcagtcc ggcggcggcc acgttcatcg ctgttgccat ggctgcagca gtgatgagca
     2341 gtgccgacag cggattgctc gctcttgcct cggtttgcgg gaagaacctc ctgcagctgt
     2401 tgaaccctaa ggctggggat ggggatgttc tgaagtggac gaggagatgg gtgaccatag
     2461 gcggtatcat aagcctcgtg atggccctct tttttcagga gattttccgc gtgcagatat
     2521 ttggccatgc tatcttagcc attgccgctt ttccaatgat gactcttgga ctctttgacc
     2581 gcaaatcgaa caatctgggc gctttgacag ggacttattt ggggatgggc gtatggatcg
     2641 gtttctctct ctttcttact catgttcagg gggcaggttt ttgggatgca tctgagatcg
     2701 ccatgcttcc ggcgctgatc gtcagttttg cgacgatgtt cgtagtatcg aggctgactg
     2761 cagctgcgga tccgcccaag tgccttgtgg atgtggacgg aaatccattg cccacttcag
     2821 gggctgtgct ttcctgcaag gctctttttc gcaggggaga gattgaatga gcatctaaga
     2881 gacggtttat caggaatggt ctttgctttg cctggacatt ggcgcggggg catctactgg
     2941 ccgcctcccg cgccaatcaa gttctgggct acatgttgac aataaggctt aagtctcccg
     3001 tcttcgcagc ctcgaggatg ggcttcatat acgtatccac ggaggatgcg tccatcttca
     3061 ccggcatgct tttcagcttc gactcgagct gtgggtcttt tgctgccgct agggcccggt
     3121 ctatgtgaga gtcggtgaac cctggcagct ctcctagagt gattgggaat ccggcttttc
     3181 tgctgaaatc gatcatgccc tcggcaacgg cgattccaag gtcacgtccg aagagtgtgt
     3241 cgatatcggc ggaaatgtag ccgtaacgtc tgtatatgtc gcccactatc cgtagctgtc
     3301 tttctatggc cggagcgaag aagacggtgt aataggggtt cattactgcg catgcccgtc
     3361 cgtgggtagt gatgtccacc agggagaagc tcgtgagatg tccgccgttt gtggcgccga
     3421 ccatgattgc gtacccgccc aagtctgttg caagtcccaa cgcttctctg ggctcgtcgg
     3481 cctccggggc atctatgact ttaggaacac tgtctactat gagctcgatg ccgatttcgg
     3541 ctatctcctg tgcaagctcg tagctttcgg ggcctatgcc gtagaagacc tcaaggcaat
     3601 gggagagccc atcgagggct ccgtccaagg tgacgtcctg tggagcgctc ttggtgaccg
     3661 tgtagtcgaa cacggcgcgg tcgggagtaa ctgcgtcgtc tattatcagt ttcttctgtc
     3721 gttccagggt gtaggtgaca tttgcatact tggtgagatg cgctcctgag cctgaggctg
     3781 tctgaactgc gatcatggga agaagcttgg cgcctgtgcg cgacatcgct ttgcttacca
     3841 gccctacgcc taagtaggcc ttgagctctg gttcgtatgg gcccaggcag gctgtaacgt
     3901 ttgcggcttt cactgcgtca atggtgcttc cgccgcccac ggccaccagg cagtcgggct
     3961 tgaaatgcag tatgtaagtg gctattcggt acacgtcttc tacaggcgaa ttaggtgctg
     4021 agcctggaac gatgcggcct tcggcaatgg agacgtcgtt ggcctcaaga gatgatgtta
     4081 cggtgctaag tattggtgca agccatgggc tgtggttggc tatcacaagg acgtccttgc
     4141 cgaactgggc tgcgaatgcc ccaacttgat gcaatgagtg ggaataggaa tctcctttga
     4201 attcgtgcag cagtctgcgg gctctggttt tcagttgctc agttcgagtc atgcatatcc
     4261 cccctccgtt tgcgtgtgtg cactgtttgt cttctaccgg tccgggagag ccttctcgaa
     4321 tttccatagc tcctgtttgc tcgtggagac ccccaaagcg ccggtggaga ctacggcctc
     4381 cagttccggc actgtttcta cgagtcctcc tgcgatgaat ggcgatagct tccttatggg
     4441 cagacggtgg atgagacttg gcaggacgag ggccggaagg atctccactg cgtccggacg
     4501 agaagaggag actatgtcga ttgcggtttt caacgattcg gagtcaagca ggaaaagacg
     4561 ttgaatggct atcaatccgg aggccttcgc cgcccttatc agattgctcc gtgtggtgag
     4621 tatgccggaa atgcctatct catcggccag ccagtcgatg cctgcagggt cttttccgat
     4681 tccctggatg agatcgatat ggcagaagac agcgatacca tagtctttgg cgctattgca
     4741 cacatctcgg aggtcacgga tagtgcctgt gaggaaaaac accagtttcg cgtcgcactc
     4801 tgctgcaagc tttaattcct ttgcctgctt gagggcgggt atcgccgggc gtcgcttgag
     4861 atggcggaat atgaggtttg tgtcggctgc tgagattctc ggcttgcttc ccagcatgca
     4921 cacctccttt cattttgtgt tgctcagtct gatcagatat gaaaagggcg accttcggcc
     4981 tgcctcaaca cgaaaccttc gacgcatgtg ggcgaaatcc tttgaacctg gttgcccgta
     5041 aaaaaggata tctccgggtt gagctggtat ttgtgaatat gttggatggg tcaggaagat
     5101 tgaaagggaa gacgccagac gagttgaagt atacaaacac agtgatgcaa cgggaaaatc
     5161 ccgatgtaat ggaagctcca gaggatatac agcgggggga attgatatgg gaagaacggt
     5221 gtgtccaccg gacgtgaaac agagggcgag cggggcgaag gccgtgttgg tgtgggcggg
     5281 actactagca ctgatttttg cggcactggc gcggcccgca gggagtcttg cggcttcgct
     5341 cgatgcaagt ggcgatgtcc gtatatatct tgttgatcca tctgacgcag agccggcttt
     5401 tgcaatcgag catgatggac ttgagctcat tgccgagttc ggttccgatg agaacagcct
     5461 gtttgtgggg gcaggctcgc tcagatgggg gccgggcaga tcagggtctt tgatgatgtc
     5521 aggaagggcg gagcctatgc ctttagtggg gtatcacctc agcagaggca attccgacta
     5581 tgttaggttc atcggactca tggaatccga tgaggcaaga aggcttatag gtcataggct
     5641 ggaatacacc cccacccccg atgttgtgct gggagtaagt gaaaccgctg tggtctcgca
     5701 gaacgcgtcg agggcgcttt atctgccgtt tcccgggttt ccactgtatg cattgcagcg
     5761 tgtggtgagt cagcaggacc gggcccagga cagtctgatc aatatcaact tgggggccga
     5821 cttcagtctg ttttgggacc tgatgtcgag cgattttgcg aaatggggcg gctttgcgcc
     5881 ccgcatggaa ttgtacggcg agatcttcat cgacgatgcg caggggcaca tacccaaccg
     5941 agcctacgtt cctgacatgg tcggcgggtt ggtcggcatc gaccttccca agctgccatg
     6001 gaacttgcgg ttcggcgcca acatcgagta tgtagcgata gcaaatttcg tctattcgca
     6061 cagaaacccc aacaacgatt atgtatatcg aggcgtcatg ctagggcatc ctttggggcc
     6121 tgattcggac atgcttgttc tcactctctc ttactgtccc gataaatgtg cgaaactgac
     6181 gctttcgggt gcggtggaaa ggcatggaga gggcagggca gggcagtcat ggtcgcacca
     6241 gcacgagccg gaggactggt ttctttcagg ggttgtggag acctgtgcca gggctgtgtt
     6301 tgggatagag cgagaactcg cccctatggc gcgtcttatg ggcagcttag aatatgcgac
     6361 caaatccaac agcggcaatg tattgggaca gtcgtctgac gagtggaata tgaggatagg
     6421 ggtgggggtc aagctttagg gggcccatcc gtgcaaggga aaaggggcgg gtggtccgcc
     6481 cccctagaac gataccctca tgccgcatct gagttttccg tagtttttcg gagacggcat
     6541 ccactcgtat tcggcgaatg cggctccgaa ctccgcgccg gccaatacgt tcggataaaa
     6601 tcggctgctt tggagttcgt agctgatccc tgccccaaca tacccggcgg tcagcacgaa
     6661 gatggggttt ttgtacatgc agattgcgga accagtggtg taggcgctat tgtggcacat
     6721 gtcgaaatta agagcaaatc gccatgtatc cgtaatgcgc attctgcaac cgaggtttat
     6781 gccgtcaacc actatccacg atgcgtgtag cgactcgagt tgcggggcgc atagagcgga
     6841 tccggatgcg cacgtcaata tgaggatgac tgtggctgat gtcaataaaa gaagaactcg
     6901 tccattcatt tcctcacctg tatatgacat aatgttcaat gcatccccat atcaggggat
     6961 atcatcttac tgcgtaatta gccgaatgta ccaggaatcc tcctgatcag tatttcggca
     7021 acggatggag gatattggat tgaggcgaaa ttgcgatatg ggaggttccc gaggctcggc
     7081 gtggatatgg tgggctttgg cgttggcata tcccgcgttc ctgcttattg catcggtgcg
     7141 gcctgagttc ggcgctgaag caacccgcgc cttcatagag aaaatactgc cacacctcga
     7201 tccaagccag gtgcacgtgg ttgtggtggc ttttcgcaag gcatgtcact ttcttggata
     7261 cggatttttc gctattgtgc tggctaatgc catccatact ctgcgaaaag gcgtgcgcca
     7321 taggaaatgg gcggttgcct cctgtataat cgcggctctg atcgccttgg gagtggctgt
     7381 actggatgaa cagattcaat cggtcgttcc ccataggacg ggcgcgagta gggatgttgc
     7441 ccttgatttt ctgggaatcg tcgtcggaat gttggtcaag gtgggcaggg ggttttgatc
     7501 ttccaaaggc gctttggggg acgtggtcat gctttttgtt gacggtttag agcgtctagg
     7561 tagggatgag aagatctggc tgatggctca gatcaacgcc ttgacaagcg cgaaccttgt
     7621 aatggacggt gttgagaaaa gcgccagagc cgtgaaagac atcgct
//
LOCUS       DAOSYL010000086         7461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_8412_length_7461_cov_5.324872, whole genome shotgun sequence.
ACCESSION   DAOSYL010000086 DAOSYL010000000
VERSION     DAOSYL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8412_length_7461_cov_5.324872"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLU15_10020"
     CDS             complement(<1..343)
                     /locus_tag="PLU15_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO31287.1"
                     /translation="MSRADQSYNRDGQPMLKVLLVEDDPMVLEIHDTIVSTVPGFTVV
                     GHARDGRAALDMIPKLKPDIIILDVYMPGRDGIDVFKEIRSLDTPVDVIMVTAAEDSS
                     TVVTSRRLGAFD"
     gene            complement(330..2021)
                     /locus_tag="PLU15_10025"
     CDS             complement(330..2021)
                     /locus_tag="PLU15_10025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO31288.1"
                     /translation="MKLGRFLLLQDRVFLFAIVVIVFAIATAGAVSIWTMKSELENRS
                     AECAVSIARTFALATPVRENLGKPGGASVIQPMAEAVSCASGAQFVVTIDMEGIRYSH
                     PIPDRIGKHVAGGDEDRSLAGEEYVSRACGTMGPSMRAFVPIYDPSGKQAGAAVVGYL
                     VDDMSREVRATLKKMYAALFVSLVVGMIGSCVLAQNVKKAMFGMEPFEIASLLEQQDA
                     ILSSVREGIIAVNADAKITLVNDEAKRMLSIEEDIVGMPVAEAIPTTRLPEVLATGKP
                     EYDREQVLGRTLIVTNRVPVVVNGKAIGAVASFRDNVEVKKLAEELTGVRKYVEALRI
                     QSHEFKNTLHAVAGMIQMGRYDDALDCIIQVSESRDKLVNFASSRIRVVPLAALLISK
                     AGAAREQGVDFCISPDSRLDEVPTCIDVHDLVTVVGNLLDNAMEAAACDPGSGAGRRQ
                     VRVTLRQAEDGTHIEVANTGPAIPDDVAQRMWDHGFTTKSDGHGIGLALVKDRVEAAR
                     GRIWFERVQLEHDSGIDKEYMKTTSGETSSETEPPVEGGTVFYVFLPVEDGHEQS"
     gene            2372..3751
                     /locus_tag="PLU15_10030"
     CDS             2372..3751
                     /locus_tag="PLU15_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOO31289.1"
                     /translation="MSKQEIKILSATGIVGYGFSEDSLRRGMERKPDVLASDAGSTDP
                     GPFYLGSGTAFVSREACKRDASLLINAARDANIPLIIGTAGGAGGDENLQWFLDIVME
                     ILRENDLHLKLAVIHSEQDKSYIRSKMKEGKVKSMGALPELTDEEIDAAEHIVGLMGA
                     EPFIAAVELGADIVITGRSSDTSIFASMPIKQGFDPGLAWHAAKLLECGAMSAEPANA
                     GDCILVTMRRDHFLVEPLNPAQRHTTVSTAAHALYENASPYHLYEAGGMLDLSDAKYE
                     QVDERTVRVSGSKFIPDKTYRIKLEAATKMGYRTISIMGTRDPILIDQIDDYLANVRN
                     TVQRRIDDAFKGEVKQSDYRLGFRVYGKNAIMGEWDPNKNTTPNELGIVIDVVADTPE
                     LSKVILAFSRTFTLHHDFPGRKCIAGNMAVAFSPSDIPMGPVYRFSMEHLIEPNDPYE
                     MFRTEIFDV"
     gene            3775..4083
                     /locus_tag="PLU15_10035"
     CDS             3775..4083
                     /locus_tag="PLU15_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOO31290.1"
                     /translation="MKLKDLAKVIRSKNAGPFYVTLDVMFSDAETYQKVKATGTLNAD
                     LIASLYNVAPDKVRFFEYDPAYSFKATIPRRIVSGDFGDTDSYGAQQHAPLLDVDISL
                     "
     gene            4121..5413
                     /locus_tag="PLU15_10040"
     CDS             4121..5413
                     /locus_tag="PLU15_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HOO31291.1"
                     /translation="MCQNQTRILGMEIKYYLVALAICIIGMAFDLVGNGMIFAIPMLL
                     VLSHGLTKIGDNIPIWKDWVGGGVTLTMFAGAILVKFIPETALESIKNFYVVDDFLVF
                     TIGNLLICSIMGMDRKVLTQAAVGFLPTVLAGVACAYLCGGLIGALLGYGFWDAILYI
                     SQPIMGGGMGAGAIPISKIYASAGLKDADAYLSMLVAAVVLGNIISIIVAAVLNKVGE
                     LRPELTGNGQLVKSAKSATQSESKVAEAQSVHLTPESIGMIIVWIGVFIILGNLARKI
                     IPVNIHPYAWMIILACAAKILNLFPEKFAQTARTYLPKYVALAMPPIMLGVGLFYTDL
                     EQFAASVSNPTFVLICLATVLGAVLGAGLFGRLFKFNFVESSMAAGLCMANMGGSGDL
                     AVLSAGKRMGLMPFAQISSRLGGAIVMIIAGAMLSLLR"
     gene            complement(6308..7225)
                     /locus_tag="PLU15_10045"
                     /pseudo
     CDS             complement(6308..7225)
                     /locus_tag="PLU15_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1806 a         1993 c         1839 g         1823 t
ORIGIN      
        1 aatcgaaggc tccgagtcgc ctcgatgtta ccactgtaga tgaatcctct gcagcggtga
       61 ccattatgac gtcaactggg gtgtcgagac tccggatttc cttgaataca tctattccgt
      121 cacgaccggg catgtagacg tcgagaatga taatatcagg cttcaactta ggaatcatgt
      181 ccaaagctgc ccgcccgtca cgggcatggc ctaccactgt aaacccagga acggtggaca
      241 cgatggtgtc atgtatctcc aaaaccatgg ggtcatcctc aactagcaag actttgagca
      301 tgggttgccc gtccctgtta taactctgat cagctctgct catgtccatc ctccaccgga
      361 aggaatacgt agaagactgt accgccttca acaggtggtt cagtctcgct actcgtctct
      421 ccgctcgtag tcttcatata ctccttatca attccagaat cgtgttccaa ctgaaccctt
      481 tcgaaccaaa tgcggcctct ggccgcctca actctatcct tcaccaacgc aagccctatt
      541 ccatgaccat ccgacttcgt agtaaaacca tgatcccaca tgcgctgcgc cacgtcatcg
      601 ggtatagcag gccctgtgtt ggctacctca atatgtgtcc catcttcagc ttggcgaagt
      661 gtaacacgca cttgacgcct gcccgcgccc gatccgggat cgcaggcggc agcctccatg
      721 gcattgtcca gaaggttgcc aaccacggta acaaggtcat gcacatctat gcatgtcgga
      781 acttcatcaa gcctgctgtc aggtgagatg caaaaatcta ctccctgttc acgcgcagcg
      841 ccggccttgg aaatcagtag agcagccaga ggcacaaccc gtatccggct ggacgcgaaa
      901 ttaaccagct tgtcacggga ctctgatacc tgtatgatgc agtccagagc atcatcgtat
      961 cgtcccatct gtatcatccc tgctactgca tggagagtat tcttgaactc gtgagactgg
     1021 atacggagcg cctccacgta tttcctgacg ccggtaagct cctctgcgag cttcttaact
     1081 tcaacattat cacgaaagct ggctactgcg ccgattgctt tgccatttac aacgacagga
     1141 actcgattgg tgacaatgag tgtacgtcct agaacctgct cccggtcata ttcaggtttg
     1201 cccgtagcca gtacctccgg aagtcttgtc gtcgggatcg cctctgctac aggcattcct
     1261 actatatcct cttctatgga cagcatccgc ttcgcctcat cgttgaccaa cgtgatcttg
     1321 gcgtctgcat tcacagctat gattccttct ctgaccgatg aaagtatggc gtcttgctgt
     1381 tccaacaatg aagcgatttc gaacggctcc atgccaaaca tggccttctt cacattctgc
     1441 gccagcacac acgaaccgat catgcctacc accagcgaca caaagagcgc agcatacatt
     1501 ttcttcagtg tggcgcggac ttcacgactc atgtcatcga ccaaatagcc cactacggcc
     1561 gcccctgctt gcttaccgga cggatcatag atgggcacga acgcgcgcat ggatgggccc
     1621 atagttccgc aggctctcga aacatattct tcacctgcaa gagatcgatc ctcatctccg
     1681 cccgcaacat gcttgccgat acggtcaggg atagggtgcg agtatcgaat gccctccatg
     1741 tcaattgtga ccacgaattg ggcaccggaa gcgcagctca cagcctcagc cataggttgg
     1801 attacagatg ctccgccagg tttgccaaga ttctcgcgga ccggcgtagc cagcgcgaag
     1861 gtccttgcta ttgagacggc gcactccgct gagcggtttt ccaattcaga cttcattgtc
     1921 cagatcgaca cggctccggc agtagctatg gcaaaaacga tgactactat ggcaaataga
     1981 aataccctgt cctgaagaag caggaatctt cctagcttca tctccaaccc agcccttctt
     2041 cgatgcatac cagcaagaaa caaccctatt gtctcactcg cgcctctgat gtggcaactg
     2101 ccacatcgcg ggatgaagaa gcaatgctgt ccattcttgg catatcccct acagctcgag
     2161 caggatgatg cctatataac cccacaataa gcctactggc acgatcgtgc gctgccaagt
     2221 ttctgcaagt atttaaaggc ctttcgaaac taatgaacac ggttagcagg gcagtctgtg
     2281 tgctagattt gaactggtgg gttgtctatt catgcaagaa aacctcacct gtattcaaca
     2341 cagcttctac tatgttacgg aggtgttcca gttgtcaaaa caggagatca aaatcctctc
     2401 tgccacaggc atcgtaggat acggattctc tgaagactcg ctccggcggg gaatggaacg
     2461 aaagccagac gtgctggcat ccgatgcagg ttcaacagac cctgggccat tctacctggg
     2521 ttctggaaca gcatttgtgt cgcgcgaggc atgcaaacga gatgcttcgt tgctcataaa
     2581 tgctgcacgc gacgccaaca tcccgctgat cattggaacg gcaggcggcg ccggaggaga
     2641 cgaaaacctc caatggttcc tcgacatcgt aatggagata ctgcgtgaaa atgatctcca
     2701 tcttaaactg gcagtaatcc attctgaaca ggacaagtcc tacatccgct ccaagatgaa
     2761 agagggaaag gtcaagtcca tgggagccct gccagaactg acagatgagg agatcgacgc
     2821 agctgagcat atcgtcggcc tcatgggcgc agaaccattc atcgctgcag ttgagctagg
     2881 cgccgacatc gtgatcacag ggcggtcaag tgacacatcg atcttcgctt cgatgcccat
     2941 caagcaaggc ttcgacccag gtctcgcatg gcacgcggcc aagcttcttg agtgcggagc
     3001 aatgtctgcc gagccggcaa atgccggaga ttgcatactg gtcaccatgc gccgtgatca
     3061 cttcctagtc gaaccgctca acccggccca gcgccacacc accgtcagca cagctgcaca
     3121 cgccctatac gagaatgcca gcccatatca cttgtatgaa gccggcggca tgctcgacct
     3181 gagtgatgca aagtacgagc aggtggatga gagaacggtc cgggtgtcgg gcagcaagtt
     3241 catccctgac aagacttatc gcatcaaact tgaagctgca accaagatgg gctacaggac
     3301 catatccatc atgggaacgc gggacccgat tcttatagat cagatcgacg actatcttgc
     3361 gaacgtcaga aacacggttc aaaggcgcat cgacgacgca ttcaagggcg aagtcaagca
     3421 gtctgactac cgtcttggtt tccgtgtgta tggcaagaac gccattatgg gcgaatggga
     3481 tccgaacaag aacacaaccc ccaacgaact gggaatagtc attgacgtag tcgcggatac
     3541 gcccgagttg tcgaaagtaa tacttgcttt ctcccgaaca ttcaccctgc atcatgattt
     3601 cccaggccga aagtgcatag ccggcaacat ggccgtcgca ttctcgccct ctgacatccc
     3661 aatgggacct gtatacagat tcagcatgga gcatctgatt gagcccaacg atccttatga
     3721 gatgttcagg accgaaatat tcgatgtgta acgaagctga ggaggcataa tgcaatgaaa
     3781 ctcaaagact tggcaaaggt catccgcagc aagaatgctg gtcctttcta cgtgacactc
     3841 gacgtaatgt tctcagacgc cgagacgtat cagaaagtca aagcaactgg aacactcaac
     3901 gcagacctga tagcgagcct ttacaatgtg gcgcccgata aggtcagatt ctttgaatac
     3961 gacccagcat attcgttcaa ggcgaccatt cctcggagaa ttgtcagcgg agatttcggc
     4021 gataccgact cttacggtgc acaacaacac gcacctctgt tggacgttga catttcacta
     4081 taagacagag caatgtatga agcattggag gcaatcagcc atgtgtcaga atcagactag
     4141 aatactgggc atggaaatca agtactatct agttgcgttg gccatatgca tcataggaat
     4201 ggcgtttgat ctcgtcggca atggcatgat tttcgcgata cccatgcttt tggtcctaag
     4261 tcacgggctg accaagatcg gcgacaacat accgatctgg aaggattggg tgggtggagg
     4321 ggttaccctc acgatgtttg ccggcgccat attggtcaag ttcatccctg agactgctct
     4381 cgagagtatc aagaattttt atgtcgtaga cgacttcctt gttttcacca ttggcaacct
     4441 gctcatctgc agtatcatgg ggatggacag gaaagtgctt acgcaggccg cagtcggttt
     4501 ccttcccacg gtgctagcag gagtagcttg tgcctatctt tgcggaggat tgatcggcgc
     4561 gctgcttggg tatggattct gggatgccat actctacatc tcacagccca tcatgggagg
     4621 aggcatggga gccggcgcaa taccgatatc caagatctac gcctcagccg gcctcaagga
     4681 cgcagacgca tatctctcca tgctcgtcgc cgcagtcgtt ctaggaaaca tcatatcgat
     4741 tatcgtagcg gctgtcctta acaaggtggg tgaactgcga cccgaactga cgggcaacgg
     4801 ccagctggtc aagtcggcca aatcggccac acagagcgag tcaaaagttg cagaagccca
     4861 atccgtacat cttacgcccg agtccatagg catgatcata gtgtggatag gggtgttcat
     4921 aattctgggc aatcttgcgc gcaagattat ccctgtgaac atccacccct acgcatggat
     4981 gatcatcctt gcatgcgccg caaaaattct gaacctgttc ccggagaagt ttgctcagac
     5041 tgccaggacg tatctgccca agtatgtggc tctggcaatg ccacctatca tgctgggagt
     5101 aggactcttc tacactgacc tcgagcagtt cgcagccagc gtatcgaacc cgacattcgt
     5161 cttgatctgc ctggctaccg ttcttggagc agtgcttggc gccggactct tcggtcgact
     5221 gttcaagttc aacttcgtag aatcttccat ggccgcaggg ctgtgcatgg ctaacatggg
     5281 aggatctggt gaccttgcag tcctttcggc tggcaagaga atgggactca tgccatttgc
     5341 tcagatatcc tccaggcttg gtggagccat agtcatgata atcgccggag caatgctgtc
     5401 gctgctaaga taaacaccta gcaaccatat tggctctgga agtattggag tgaactgcgc
     5461 gactccctgt ccgctaaggt ggatagggag ttttatttgt gtgcccgggg tggatagcgg
     5521 cgatgacatc agcgctccca cccttgtctc ccatgtggca tattccatat ggccatttgt
     5581 gacaaggtca agtgagtgct aaagaagtcg gcacatttcc gttgaggacc aaaggaaaat
     5641 cgaggatcga gtaacgaggg gctacttcct gccctgggcg ggccgggtgg cggggactag
     5701 ggaaggggag tcttcgggat tccggcaggg tgtctagatt ttagccactt tggaaacaaa
     5761 agggctcaac ttttaggcgc ggcctgtcta aaaattcata tgaaggaggc cgtcaagccc
     5821 caatttcaat ttcggcagcg gtgctaccga agacccggtt tacgtcgacg gttggcagac
     5881 tcatatccgc gggttggtta ccgcaactaa taccaccgtc tggagctcca tcatactaga
     5941 ctcgcacgta atgcctctat gggcattgtg cgtaattttt tcgtggattc tccctaccgg
     6001 cttccggttc ttcggccacc tttcaccgaa accttggcat caatagaaaa ccaagcatct
     6061 gttcccttgg ggggtatccg tagggaacta cgttgccctg aggcaataca ggttttatta
     6121 aattgccttg gtggtcaaga ttaccgttta tgccgccctg cggccagttg ccatttcatt
     6181 tgggattatg cacctatgta tagctataag ttgcatgtaa ttgcgttgac atcccgttga
     6241 ttcccagtgt atcccgggtc tgtaaatact attgtgcaaa tggtgttttc ctttcttgac
     6301 ggggagatca caagtcagtt ctcgtcatgt ctatgcggtc accaaagatc aaagcaaatt
     6361 gggacatgca aaaggcccag tcgttcactg gcactttcca tttctttaga atatccatcg
     6421 tagccagata taggactttc ctcagcgagt tctcgtctgg aaacacggac ttggtcttgg
     6481 tgactttcct tagttgcctg ttataggact ccacagcgtt agtggtgtag atcaacctcc
     6541 tgacatccat ggggtagcgg aagtaagacg taagctctac ccagttcttc tcccactgcc
     6601 ttatcggaat tgggtacccc tcgccccatt tttgcttgag tgagtcaaat gcaagctcag
     6661 cttgttttat ggtaagagcc tggtacactt gctttaggtc aatcatgaaa gccttccgat
     6721 tcttgcttgg cacgtatttg agtgagttgc ggatttgatg aattacgcac agctgcatat
     6781 ctgtttttgg gaacaccgct ttcactgcct cggagaagcc gcttagtcca tctctgtaga
     6841 ctattaagat gtcatcaacg cttctggatt tgagatcgtc gcatacttcc agccagaagc
     6901 ttgcgctttc ctcatcacca atccaagtcc ctaagatatc tttgtacccc gaggagttta
     6961 tccctaatgc tgtatatgcg gccttattga cgattctact gtcttggcgg acattgaagt
     7021 agatggcgtc cagaaaaaca atgaggtaaa cccgatccaa tggtcgtgac tgccactcct
     7081 ttatcatcgg cacgattctg tccgtggtgc tgctgacaag cccagacgag acgttgatgc
     7141 catacagctc ccttatctga tctgctatgt cccttgtaga catatctttg gcatacatag
     7201 tgattatctg gttttcaagc ctgtcaatgg ccatttgaga atcccctttt ttggccatga
     7261 aaaatcccca cgggaagagc aacgggtcta cgagcaagag cctcatgtca gcgactgcct
     7321 catatatgca ttctgtttgg caatctaaaa gttcatagtt cggcaatgaa aaaagtgcat
     7381 aagttgatca ccgtgctcga gggcggtacg gggccagtgg gctttggtaa ctgtgttctg
     7441 aatatatgag ccgccgcgtc t
//
LOCUS       DAOSYL010000087         7395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_8527_length_7395_cov_8.243324, whole genome shotgun sequence.
ACCESSION   DAOSYL010000087 DAOSYL010000000
VERSION     DAOSYL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_7395_cov_8.243324"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1646
                     /locus_tag="PLU15_10050"
     CDS             279..1646
                     /locus_tag="PLU15_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO31292.1"
                     /translation="MKSASVRDLLFPTTIAWKTYVIVIAASLVPALLAVALGGRDLAQ
                     ARPEGYERELSSVCRLLDAELESRHGSFAHLLETEGALAAPREKQIQLLNAELQPIVD
                     MISVAYPDVEFGYYSKDLRAELAIHPMTDAVNIYKEVLPEEFNIYRTGRPEVLRPSVS
                     EAQKAQGGMAVQTCPLYDGDELVGHVWAKMPVRIEHKRLYTAGSLVGLAAVTAAVLLV
                     SSWYIMRGLGHWEERYALSRGAMANREAAAIVHEMRNPITVSKGLVQLVLTQETDMRK
                     RMWLSNIVRQLDHMNNIASDYLQYARPRPPRVSDVSVQELVEDLVENVAPYVEESGAI
                     MEVSSDCEDLKVRCDRDQVGQVLLNLVKNAVEAGLGEPGLKVAIRWRREGGTVIIEVA
                     DNGPGIPQENLSRIFDPFFTLKPKGTGLGLAISRTLIEQQGGSLAAHSSQAGGAVFSV
                     KLPRA"
     gene            complement(1808..2107)
                     /locus_tag="PLU15_10055"
     CDS             complement(1808..2107)
                     /locus_tag="PLU15_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOO31293.1"
                     /translation="MIKLAYEDKILNCRDCGQDFVFTAGEQEFYDQHGFQNEPSRCPD
                     CRRARRASSGSGNRQMYEAVCSACGKPAYVPFQPSGDKPVYCSECFAAQKSMRNR"
     gene            2568..3026
                     /gene="tadA"
                     /locus_tag="PLU15_10060"
     CDS             2568..3026
                     /gene="tadA"
                     /locus_tag="PLU15_10060"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOO31294.1"
                     /translation="MKQELLDVKFMSEAMAQAHEASLAGEVPVGAVVVMGDEVIARAQ
                     NRKESDKDPTAHAEILAIREAANRIGSWRLSGATIYVTLEPCPMCAGAIVQARLDRLV
                     YGAADPKTGAAGTIFDLPRDMRLNHWVSVTGGVMAEEAGALLEKFFSNKR"
     gene            3046..3136
                     /locus_tag="PLU15_10065"
     tRNA            3046..3136
                     /locus_tag="PLU15_10065"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3081..3083,aa:Ser,seq:cga)
     gene            3175..3265
                     /locus_tag="PLU15_10070"
     tRNA            3175..3265
                     /locus_tag="PLU15_10070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3210..3212,aa:Ser,seq:gga)
     gene            3304..3394
                     /locus_tag="PLU15_10075"
     tRNA            3304..3394
                     /locus_tag="PLU15_10075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3339..3341,aa:Ser,seq:gga)
     gene            3417..3681
                     /gene="ffs"
                     /locus_tag="PLU15_10080"
     ncRNA           3417..3681
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLU15_10080"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            3831..5561
                     /gene="dnaX"
                     /locus_tag="PLU15_10085"
     CDS             3831..5561
                     /gene="dnaX"
                     /locus_tag="PLU15_10085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOO31295.1"
                     /translation="MTYVSLYRRWRPQTFDDIVGQGHVVRTLKNAVSTGRISHAYIFS
                     GPRGTGKTSTARVLARALNCEKGPTPDPCGECRSCVAIRDGNAMDVIEIDAASNRGIE
                     EIRDLREKVVYAPTQGRYKVYIIDEAHMLTQAASNAFLKTLEEPPSHVIFVLATTEPE
                     NILETIRSRCQRFDFNRLSVSDLADHVSRVAAAQSISIDPDAAKLIARRADGSARDAL
                     GLLEQASAWSTHVTSESVLELLGTSDFDTLYNYADAARRGEPGQLLSIIRDVVESGAD
                     MRQFLQDLVVHFRNLLVAKECPDLGGLLDAPEAVQASIAEQAAFFTRERIIEILDILS
                     EAEIQVRRSPNPRLLVELAAAKICAEPREDAVAQVGRAQKDTESFAGVQAAETRRKGL
                     EQAATLPDRKGSPGAGGSLDASGAGRRDGGEEAKPQGQAKGARGTGDARQEQNAQSGY
                     VGISEEEWNEALAHIKKANLVLEAFLKPVTAVCVDGDVATIMYDPSWTVHVQKASEPD
                     NKELIARALTDVLGRPVQCRVMAKAPQTKAKPSGEGPDGGGFGDYSDVPEVLEAIKLF
                     DPVEIKVIED"
     gene            5583..5900
                     /locus_tag="PLU15_10090"
     CDS             5583..5900
                     /locus_tag="PLU15_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOO31296.1"
                     /translation="MGGNMKKMMKQVQKMQEDMERVQQELAEERIETTAGGGVVRAVV
                     NGHQEVLEIHIDPAVVDPDDVDMLEDLVVAACNEALRKAKELAEQRLAKVTGNISIPG
                     LNF"
     gene            5927..6526
                     /gene="recR"
                     /locus_tag="PLU15_10095"
     CDS             5927..6526
                     /gene="recR"
                     /locus_tag="PLU15_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOO31297.1"
                     /translation="MLLYPEPVGRLIEELRRLPGIGQKTAQRLAFHILSMPEAEVKSL
                     SDALVQAKEKTRSCSVCGNFTDSDPCAICGDGSRDQSTICVVERPEDVIALERTREYR
                     GLYHVLGGAISPMEGVGPSDIRLRELLERLKSDVVCEVILATDPNIEGEATAIYIAKL
                     LKPLDIRVTRLAHGLPVGGDLEYADEVTLARALEGRRGM"
     gene            complement(7103..>7395)
                     /locus_tag="PLU15_10100"
     CDS             complement(7103..>7395)
                     /locus_tag="PLU15_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31298.1"
                     /translation="SFTLFGFQGTSAALAPHRFSPAAAGRHLLSYHPSSNMSTPILRF
                     RFCLIVAAFRQTKCFCLLKQISQPAFARTHSEYGSIYQFWFSNIVKYEIGLF"
BASE COUNT         1660 a         1799 c         2266 g         1670 t
ORIGIN      
        1 tatgccatca tacggagcaa agatggttca aatgcccatg ctgttatggc caccgcatcg
       61 ttttgactgg caagccgctt gtagaaaggg gataagacac gcagcgacag cagtgccatc
      121 atcctttgtc ccctacggct gggtgcaaca gcttcattat gtggcaacga gtgggatttc
      181 ccgtcagtca tgaggagtga agaatctagg gcggccatat ggttgccctg ttgcacccat
      241 actgatttgg acaaggtttt cgcggggagt gtagtgcatt gaagtctgct tctgtgcggg
      301 atcttctctt tcccacgact atagcgtgga aaacatatgt cattgtcatc gctgcgtcac
      361 tggttccagc gctgcttgct gtcgccttgg gtgggcgaga cctggcgcaa gctcggcctg
      421 agggatacga gcgggagcta tcctccgtat gcagactgct agatgccgag cttgaatcgc
      481 gccatggatc ctttgcgcat cttctggaga ccgaaggcgc tctcgcggcg cctagggaaa
      541 agcagattca attgctaaac gctgaattgc agccaatagt cgacatgata tctgtggcct
      601 atcccgacgt tgagtttggc tattattcca aagaccttcg ggccgagctt gccatacatc
      661 ccatgaccga tgctgtaaat atatacaaag aagtgctgcc tgaggaattc aacatatatc
      721 gtactggcag acctgaggtg ctgcgtccat cggtatctga ggcccaaaag gctcagggcg
      781 gcatggcagt gcagacatgt ccgctatatg atggggacga gcttgttggc catgtgtggg
      841 cgaaaatgcc ggttcgaata gagcataaga ggctatatac cgcaggcagc cttgtgggac
      901 ttgcggccgt aaccgctgcc gttctcttgg tttcatcatg gtacatcatg cgcgggctgg
      961 ggcactggga ggagcgatac gcgctcagcc gtggtgcaat ggctaatcgt gaggctgcgg
     1021 cgattgttca cgagatgcgc aacccaataa ccgtctccaa aggcctcgtc caacttgtat
     1081 tgactcaaga gactgacatg agaaagagga tgtggctttc gaatatcgtc cgccaattgg
     1141 atcacatgaa taacatagct tccgattatc ttcaatatgc ccggcccagg ccgccccgag
     1201 tatccgatgt ttctgtgcaa gagcttgtag aggatcttgt ggagaatgtt gcgccatatg
     1261 tcgaagaatc gggcgcgatc atggaagtat cgtctgactg cgaggatctc aaggtgcgat
     1321 gcgatcggga tcaggtaggc caggtattgc tcaacctggt gaaaaatgca gtggaagccg
     1381 gattgggcga gcctgggctg aaggttgcca ttcgctggcg ccgggaggga ggaaccgtca
     1441 ttatcgaagt ggccgataat ggcccaggga ttccccagga gaacctttcc cgcatattcg
     1501 acccattctt cactctaaag ccgaaaggca caggccttgg ccttgcgata tcgaggaccc
     1561 ttattgagca gcagggggga tcccttgctg cccatagttc tcaggcagga ggcgccgttt
     1621 tctccgtaaa gttgccgcgg gcgtgatccc gggaagattt gcttgcttcc cggcaggcag
     1681 aaaagtgcag tacgccttgt gtatcttcag ctgaacaacg gagaaaggaa agagtcccgg
     1741 aagtatcccg ggactctcca tgcctacgtc gaatgcttca tattcctaaa cgagcttttc
     1801 gagttgccta cctattcctc atcgacttct gcgcagcgaa gcactcgctg cagtataccg
     1861 gcttatcgcc gcttggctgg aacggaacgt aggctggctt tccacaggcg gagcagacgg
     1921 cctcatacat ctggcggtta ccagaaccac tagaggccct gcgggctctc ctgcaatccg
     1981 ggcacctcga cggctcgttc tgaaatccgt gctgatcgta aaattcctgc tctcccgcgg
     2041 tgaacacaaa atcctgtcca cagtctctac aattgaggat cttgtcttcg tatgccaact
     2101 tgatcatccc cgattcgttg gatagtctct tgatcaagtc gacaggcatt agaatgcgtg
     2161 cgcttgaaca acagccatat atatcttaaa gcctttccgg cgaacttgca atatgctgcc
     2221 aacaaaagta ttgtctgacg cttcgtgccc acgtgagtgt caagcccact tttgcctggt
     2281 agagctgggg gttgtccgag aacctcggga aattgtggcg aaaagaaact tcccgctcga
     2341 taccttatgt atggccattg ttcactctta accatagatt tgctgcctga tctgagcttc
     2401 tgtaggaaat aatgcaccgc agctttgacc gattttccag gtcctgtgcg ccgattattg
     2461 cgtttgccga agatgcccgt tcgccgggag ggaatgcgga ggtagtgttg atttaccctc
     2521 atcggtatgg taacatatgt ggcgagcaaa ggatgcttca tagaactatg aaacaagaat
     2581 tgcttgatgt caagtttatg agcgaggcca tggcccaggc gcatgaggcg tcgttggctg
     2641 gcgaggtgcc ggtcggcgcg gtagtggtta tgggcgatga ggttatcgcg cgcgcgcaga
     2701 accggaagga atcggataag gatccaacgg cgcacgcaga gatacttgcg atccgggagg
     2761 ctgccaaccg catcggatca tggcggcttt cgggggcaac catttacgtg acgctggagc
     2821 catgtcctat gtgtgcaggt gccatagttc aggcgaggct tgatagattg gtttatggag
     2881 cggctgatcc caagacaggg gcggccggta ctatattcga cttgccacgc gacatgaggc
     2941 tcaaccactg ggtgagtgtg acaggcgggg tcatggcgga agaagccggc gcgttgcttg
     3001 agaagttctt ttcaaataag cgttgatatt tttcatatag cttgcggaga gatggctgag
     3061 ctggtcgaaa gcgctcgcct cgaaagcgag taggcgggga accgcctcgt gggttcgaat
     3121 cccactctct ccgccataga ttctgaggcc tatttctggg gctttccata ttgcggagag
     3181 gtggctgagt tggtctaagg cgctcgcctg gagagcgagt aggcggggaa ccgcctcgtg
     3241 ggttcgaatc ccactctctc cgccatagat tctgaggcct atttctgggg ctttccatat
     3301 tgcggagagg tggctgagtt ggtctaaggc gctcgcctgg agagcgagta ggcggggaac
     3361 cgcctcgtgg gttcgaatcc caccctctcc gccatttttg ttcatattga ctttggccgt
     3421 gctagacggg gaggtagcgg tgccctgtac ccgaaaaccg ctatagcggg gttgacttcc
     3481 caccctaggc ctgccttctt tggggcctgc ccgacgcaag tggtgttgag ggctgggtcc
     3541 tgcgcaacga ggctctgtga accccgtcag gaccgggagg tagcagcggt aagcagatac
     3601 cctcgtgtgc cgcgggagcg cctggtcgga gctggctacg tcggtaccgc ctggggaagc
     3661 ttgtcgaagg tgggtgcacg gcctctacat cgtcaggatg gtccggctgg gccatccttt
     3721 tttgatgtgg aggacaaaga gagacctgga gcgaatacta cttgaggcat gttttgctga
     3781 gcctgctttc tagggcttgc aaaggcaaat cggattttgg ggggctaccg gtgacttacg
     3841 tttcgctcta ccggcgatgg cgtccacaaa cgttcgacga tatcgttggg cagggccatg
     3901 ttgtcagaac cttgaagaat gcggtgtcaa cggggcgcat ttcccatgcc tatattttct
     3961 cagggccaag gggaactggc aagacttcta ctgcaagggt gcttgctcgc gctcttaact
     4021 gcgagaaggg accaacgcct gatccgtgcg gagagtgccg ttcatgtgtg gcgatacgcg
     4081 acggaaacgc tatggacgtc atagagatcg atgcggcttc caacaggggc atcgaggaga
     4141 tcagggatct gcgcgagaaa gtggtgtacg cacctactca gggcaggtac aaggtatata
     4201 tcattgacga ggcccacatg cttacacagg ctgcgtccaa cgcttttcta aagacgttgg
     4261 aagaaccgcc tagccatgtc atattcgtct tggccacgac agaacctgag aacatcctag
     4321 agaccatcag atcgcgctgc cagcgtttcg attttaacag gctgagcgtc tccgaccttg
     4381 cagatcacgt ttcccgggtg gccgcagccc agtctatatc tatcgaccct gacgccgcaa
     4441 agctcatagc caggcgcgct gacggaagtg cgagagacgc gttgggcctt ttggaacagg
     4501 catctgcatg gtcgacccat gttacgagcg aatcagttct agaattgctt ggaacgtctg
     4561 atttcgacac cctatacaac tacgccgatg ctgcccgcag gggagagccg ggccagctgc
     4621 tttcgatcat ccgcgatgtc gtggagagtg gcgctgatat gaggcagttt ctgcaggacc
     4681 tggtggtcca ttttcgtaac ttactagttg ctaaagaatg ccccgacctc ggcgggttgc
     4741 ttgatgcgcc agaagcggtc caggcgagca ttgcggagca ggctgccttt tttacgcgcg
     4801 agcgtataat cgagatcctg gacatcctca gtgaggcaga aattcaggtg aggcgttcac
     4861 ccaatccccg tcttctagtg gaacttgcgg cggcaaagat ctgcgccgaa cctcgcgaag
     4921 atgcagttgc gcaggtcggc agggctcaga aagatacgga gtcttttgcg ggggtgcagg
     4981 ctgccgagac gcgtaggaag ggcctggaac aggccgcgac cctgcctgac aggaaaggat
     5041 cgccgggtgc aggcggcagt cttgacgctt ccggggcggg caggagggac ggcggggagg
     5101 aagcgaagcc tcaaggacaa gcgaaaggtg cccgaggcac cggggatgcg agacaggagc
     5161 agaatgctca atccggatat gtagggatct ctgaggaaga atggaatgag gctttggcgc
     5221 atataaaaaa ggctaacttg gtgctggagg cctttctgaa gcctgtaacg gcggtctgcg
     5281 tagacggtga tgtggcgaca atcatgtatg atccttcgtg gacggtccat gtgcagaaag
     5341 cctctgagcc cgacaataag gaattgatag cgagggccct cacagatgtg ctggggaggc
     5401 cagttcaatg tcgggtaatg gccaaggccc cgcagacaaa ggcaaagcct tctggagaag
     5461 gtcctgatgg cggcggattc ggcgattact ccgatgtccc agaggtattg gaagcgataa
     5521 agctgttcga tcctgtggag ataaaggtga ttgaagattg aatagagcgg ggaggggacg
     5581 agatgggcgg aaacatgaag aagatgatga agcaagtgca gaagatgcag gaggacatgg
     5641 aaagagtcca gcaggagctt gcggaggagc ggatagaaac cacggccggt ggaggtgtgg
     5701 tgagagccgt tgtcaacggg catcaggaag tgctcgagat ccacatagat ccggcggttg
     5761 ttgatcctga cgatgtggat atgcttgaag acttggttgt tgctgcgtgc aatgaggcgc
     5821 ttcggaaggc gaaggaattg gccgaacaaa ggcttgcaaa ggtaacgggc aatatctcca
     5881 ttccgggact caacttttag gctctagccg tcgggggaaa tcagatatgt tactttatcc
     5941 agaaccagtg ggaaggctca tagaggaact gaggaggctt ccgggcatag gacagaaaac
     6001 agcccagagg cttgcctttc atatcctctc tatgcctgaa gcggaagtga agagcctttc
     6061 ggatgcgctg gttcaggcga aggagaagac gcgaagttgc tctgtgtgtg gaaattttac
     6121 tgattctgac ccgtgcgcca tctgcggaga tgggtctcgc gaccagtcga ccatatgcgt
     6181 ggttgaacgg cctgaagacg ttattgccct ggaacgcacg agggaatacc gaggcctgta
     6241 ccatgtcctg ggcggcgcca tttctccgat ggagggtgtg gggccttcag atattcgcct
     6301 ccgcgaactc ctcgagcggc tgaaatccga tgttgtgtgc gaggtgattc tcgctactga
     6361 ccccaatatc gaaggcgagg ccacagcgat ctacatcgcc aaacttctaa aaccccttga
     6421 catcagggtc acgcgccttg ctcacggact tccggtaggc ggggacttgg agtatgccga
     6481 cgaggtcacc ctcgcccgcg cgcttgaagg aaggcgcggg atgtagcaag gcctttctcg
     6541 atcgaccaag tttctcgctg ttccgcgttg gccaggccgt gtgcggtggc gttttttcat
     6601 agttcaagcc attgagtaat agggctttgc caggtgattc gagggtcctt gcgggccacg
     6661 cgtattacga aacagctcag ttggcgaagc aatgtcgtgg gagaacacga atgcggggag
     6721 tcggaagaaa tggaattagg cctagatagg gcggggtgga agcctgaacg tgatcgacgg
     6781 catcgggccg ggcagacgtg atgcgatcct tgtagattat ccacctgata cgtgtcactt
     6841 tgattctacg tgtttgcggt agagacaatg gattttggac atgttccata ggcatactat
     6901 ctaaatggac ggcgcaaact atattatctg tgattgttcc gccgccgaca ttgcgaagag
     6961 tgaggtgtgc actatggacc caagggcgat ctacacgcgg ttggccggct cgttccgggc
     7021 tgtattcccg tctcggtcac ctgcggtcga ggattacggg cggggatggc tcaaggggga
     7081 gatacgttcg gtatgcgccc aactagaaca ggccgatctc gtatttaaca atgttactga
     7141 accagaactg atagatgctg ccatattcac tatgcgtgcg tgcgaacgca ggctgggata
     7201 tctgtttaag caggcaaaag cacttagttt ggcggaaagc tgccacaatg aggcagaaac
     7261 gaaatcgcaa aattggtgtt gacatgttcg agctagggtg gtaagatagc aaatgtcgcc
     7321 ccgctgcggc gggggaaaat cgatgcggcg ctaaagccgc ggatgttcct tgaaaaccaa
     7381 acagcgtgaa gcttg
//
LOCUS       DAOSYL010000088         7164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_8864_length_7164_cov_13.400338, whole genome shotgun sequence.
ACCESSION   DAOSYL010000088 DAOSYL010000000
VERSION     DAOSYL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8864_length_7164_cov_13.400338"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..971)
                     /locus_tag="PLU15_10105"
     CDS             complement(120..971)
                     /locus_tag="PLU15_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOO31299.1"
                     /translation="MTGSDRVKRLRRNAKRQAAVQLSLSQKALGYVRVSTDEQAAAGY
                     GLEAQEHAIRAFAESQGYELVDVILDAGVSGSTRPSDRPGFARVMELADEQAFSILLV
                     WKFDRLARSLEYAVTTVNGMQEHYNIVLRSVTEPIDTATPMGQTIFAVLAGFAAQERM
                     AITQRTLIGKREKAKKGGFVGGAAPLGYKRDRDGGLIVDEDEAETVRKIYELREDGQT
                     LQAIADVMNRESIPTKRGRRWYPGTVRYVLDNPKYKGLVEYYFRWDGEEHILQQGQHE
                     PILSQAQ"
     gene            1419..1931
                     /locus_tag="PLU15_10110"
     CDS             1419..1931
                     /locus_tag="PLU15_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31300.1"
                     /translation="MQIAFRQAETPQYEALINVAKTQGLRSLETTVLRFPSVNSNYAA
                     CSATLEAADGATYKEVACVAAASSSGDAQAKALELACQRAKLKALESFTGIKCEVETI
                     PSAPSPQPSLKAGATLVESSRAPAPLVLPTGPCSECGTELSPGNHEFSTKLFKRPLCV
                     PCQINVAASR"
     gene            2031..2861
                     /locus_tag="PLU15_10115"
     CDS             2031..2861
                     /locus_tag="PLU15_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HOO31301.1"
                     /translation="MKTNELMDQIESRIKELATRTEEAKASEEVTQYLQFLSKFHRYS
                     LHNTMSIWIHCPNATHVAGYKAWQNLGRQVRKGESGIPIFAPCTQAVPKREEEDDEKK
                     TRRVIGFKVVYVFDVGQTEGEPLPEAPITARGQADGLVPILESIARSHGITYGYQTMD
                     GSHHGTSYGGHIEIDARLDDAGKVSVWVHELAHELLHKGDDRQMLSRLQRETEAEATA
                     YVVCTHFGIDSAAPNYLALWRAEPEEIMGAFQRIRNVASTLIEAIEAEIPSYREEVPA
                     "
     gene            2858..3115
                     /locus_tag="PLU15_10120"
     CDS             2858..3115
                     /locus_tag="PLU15_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31302.1"
                     /translation="MKTVRDSDEAIKYVQRLGFTPQGNIQHQQASLCHLVTALEQAAG
                     GYPYKVVFHRHVSHAGVSFPWLGEFVIYGRRGKIQVKAKNV"
     gene            3256..3864
                     /gene="radC"
                     /locus_tag="PLU15_10125"
     CDS             3256..3864
                     /gene="radC"
                     /locus_tag="PLU15_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOO31303.1"
                     /translation="MNCNYPDLCALSAPRLAAIALRDEDVIKAFTRFNSLREMEDAPV
                     EDIMAAGLTREQAESLKAALELGKRLANEPRPKKAIFGAADIAELLGPEMSKYDREHF
                     KVVMLDTRNQVIGVETVAIGTLNMCPAHPREVFKTAILNSAAAVIIAHNHPSGSPSPS
                     PEDIALSKRMQRAGALIGIDVLDSIVIGEAPTYMSLKQMGVL"
     gene            3940..4512
                     /locus_tag="PLU15_10130"
     CDS             3940..4512
                     /locus_tag="PLU15_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31304.1"
                     /translation="MPRINDEHIITIDGKPYVKYGGLLETAHRSGLKDLQVKLIQSPS
                     RENGYTAVCEATAVIKTRSREKVYVEIGDASPASIGDPKLVPHAIRIAATRAKARALR
                     DALHISICSVEELTIGNGSAPEASAASSPAKAPQPDKVTEPQLRVLRMQAEQLGLRDE
                     DCTRFNTMTKKEASETISAFNRQLNMARVG"
     gene            complement(4859..5695)
                     /locus_tag="PLU15_10135"
     CDS             complement(4859..5695)
                     /locus_tag="PLU15_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31305.1"
                     /translation="MPPIDGVNIVRRYYRRTGVRRPVEKSKKKHRKCGSYRIMEQRGH
                     MTPYGNGSFTLCYEPEFSIMPSMTMTINPSKLLLSPQDLGAIVAQYMPIESARIAYIE
                     CKLDLEHTDAKHLADAISIKWARQRQSHSSTSFSFGGKRSRERITIYDKAVQLDIDVI
                     GLTRLEVKIRLPTKGRPTVAEFVSGCWEPRNPFAKMRLMEASGLCEVEQAARDAIADD
                     GVHNGLKTYAQITNRGRADKAKKILLFEVPELWEAWLDLLEMWYCPVADTVDDGIVAT
                     GA"
     gene            6050..6724
                     /locus_tag="PLU15_10140"
     CDS             6050..6724
                     /locus_tag="PLU15_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO31306.1"
                     /translation="MSIPRLWEITCDCLEHYCSISYGQAIELEKVSDIILSSPNINVA
                     NVNAVDKAIRQLRTAATILKKMMDSNQLAQDEFVSVYKTLQNIDTVANRLLFGSKLKN
                     VRNAKGYNVTELASSVGVDRSYISKLEKAVAGPPDIEVIAQLSYVLAVPMSEFVVDAI
                     GHISFERADNSNIVDPKNAIISDIARECEAIPEEYLVILLAQVKAISQTYRKRKFEGV
                     WRRLDQ"
     gene            complement(6745..>7164)
                     /locus_tag="PLU15_10145"
     CDS             complement(6745..>7164)
                     /locus_tag="PLU15_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31307.1"
                     /translation="SLNSLKHGLTIDGFLPCKKEGCYFYEVCGLWHSYEGRKILDATA
                     YGDPCPGEVIAYYDIRHGLAREGICDETWAHMWAMNEVHMVRRRMLSAVDVDIVREVP
                     TGFEGHVRPELALAFRYQDKLRSDRDALLRMLVCRDQ"
BASE COUNT         1774 a         1858 c         1850 g         1682 t
ORIGIN      
        1 attgtaatgc ggtgccgaag gggggattcg aacccacaat caggtttgat ttggtgcgag
       61 aggagggatt tcacccctcg tcgtgcctat atccaatagt tcactgtccg tcatcgttgc
      121 tattgtgctt gagataagat gggctcgtgc tgaccttgtt ggagaatatg ctcctctcca
      181 tcccaacgaa aatagtactc aacgaggccc ttgtatttgg ggttatcgag aacatacctg
      241 acagtgccgg gataccatcg gcgcccgcgc ttagtaggaa tgctctcccg attcatcacg
      301 tccgctatgg cttgtagggt ctggccatcc tcgcggagct catatatctt cctcacagtt
      361 tcagcttcat cttcatcgac gataaggcct ccatctcgat cccgcttgta tcccaatgga
      421 gcagctcccc ctacgaagcc gcccttcttg gccttctcac gcttgccaat tagagttctc
      481 tgggttattg ccatacgctc ctgggcagca aagccggcga gaacggcaaa gatggtttga
      541 cccatagggg tggctgtgtc tatgggttcg gtcacggaac gaagaacaat attatagtgc
      601 tcttgcatgc cattgaccgt ggttacagcg tactccaggc tgcgggccag tctatcgaac
      661 ttccagacaa ggaggatgga aaaggcctgt tcatcggcga gctccataac gcgtgcaaac
      721 cctggccgat cagaaggcct tgtgctgccc gacacccctg catctagtat cacatcaacg
      781 agttcatacc cctgggactc agcgaaggcc ctgatggcat gctcctgagc ttcaaggcca
      841 tagcctgcag cagcctgctc atcggtggat accctgacat atcccaaagc cttctgtgac
      901 agcgaaagct gcactgctgc ctgcctcttg gcgttacgtc gcagccgctt gaccctatcg
      961 ctacccgtca tgtgagccgc ctcctatggg tgatttatta attctcttga gaatttataa
     1021 ttaccaagtt tatcatacca caattggcgt attattgcca gggcaggcgt gtcatccttg
     1081 cttgcgtatt acaagcccta cgggctgttg tggatcctca atgtatctga tagagcccta
     1141 tatgagcggg aattgagaac attttctgag ggggttccta caatgatgag ttgaatccgg
     1201 aattcttaac gtgcccaccc cttgcatgac cattgattgc agggtgctgc acactgtgcc
     1261 gccggcaaca ttcctaccat gcgacggcat atggtgtttc ccacggtctg ctatatcctc
     1321 tctacatatg gttgcatacc tattgtcgag ccgtaagttc aatgccccac ccatctcaca
     1381 tgctgtgtta ataggtcatg agaaaggagg tgtgagccat gcaaattgct tttcgccagg
     1441 cggaaacgcc gcagtatgaa gcactgataa atgtggcgaa gacacagggc ctaagaagcc
     1501 tggaaacgac ggtgttgcga ttccccagtg tgaattcgaa ctatgccgca tgctcagcga
     1561 cgctggaggc tgcagatggc gctacctaca aggaagtggc ttgtgtcgct gctgcttcaa
     1621 gttcggggga tgcgcaagcc aaagcccttg agcttgcatg ccaaagggcc aaattgaagg
     1681 ctcttgagag tttcacgggc atcaagtgtg aggttgagac tatcccgtcg gcaccatctc
     1741 cgcaaccgtc actcaaggct ggtgcgacac tggtcgaatc ctccagggcg ccagcgccac
     1801 tggttctccc tactgggcca tgttccgaat gtggcacaga gctgtcccca gggaaccatg
     1861 agttctctac caagctcttc aaacgtccct tgtgtgtccc ctgtcaaatc aacgttgcag
     1921 cctcacgcta accctatagc agtatcccat atcagacgac caacgcccag ggcatcagcc
     1981 gggggcgtat gtcggcatgt acaagaacgg tgaaaggaag tgaccccaca atgaaaacca
     2041 acgagctcat ggatcaaatt gaatccagga tcaaggaact ggcgacccgg acggaggagg
     2101 ctaaggcttc agaagaggtg acgcagtatc tgcagtttct gtccaagttc cacaggtata
     2161 gcttgcataa caccatgtcg atatggattc attgccccaa tgcaacgcac gttgcgggtt
     2221 acaaggcatg gcagaatctc ggaagacagg tgcgtaaggg cgagtcgggc attcccatat
     2281 tcgcgccttg cacgcaagca gttcccaaac gcgaggaaga ggatgacgag aagaagacac
     2341 ggagggtgat cggcttcaag gtcgtctatg tgttcgatgt gggccagacc gaaggggagc
     2401 cgcttccaga agcccctatc acggccaggg gccaagcgga cgggcttgtt cctatacttg
     2461 aatccattgc gagatcccat ggcatcacct atgggtatca aaccatggac ggctctcatc
     2521 acggaactag ctatggcggg catatcgaaa tcgacgcacg tttggatgat gccggcaagg
     2581 tctcagtatg ggttcatgaa ctcgctcacg agctcttgca caaaggagac gatcggcaga
     2641 tgctgagcag gcttcaaagg gaaacagagg cagaggcgac tgcctacgtt gtgtgcacgc
     2701 actttggtat cgacagcgcc gctccgaact acctcgccct ttggagagcc gagcccgagg
     2761 agatcatggg ggcctttcaa cgtatccgca atgtggcctc aacattgata gaggcgatcg
     2821 aggcagagat accaagctac agggaggagg tgccagcatg aagacagtca gagactctga
     2881 cgaggccatc aagtatgtgc agcgccttgg gttcacgcca cagggaaaca tccagcatca
     2941 gcaggccagc ctgtgccatc tagtcacagc tttggaacaa gccgcaggcg gatacccata
     3001 caaggttgta ttccatcgcc acgtgagcca tgcaggagtc agtttcccat ggctcggtga
     3061 gtttgtcatc tatggcaggc gtggaaagat ccaggtcaag gcgaagaacg tgtagcttct
     3121 aagagatatc cataatcatc atcagctcca ctgtgtgggg ctgttttgtt gtacaggcag
     3181 ggggcggcgc cgcggtcaga gcggtagtcg ccctcttttt gtatctacgc cacagaacga
     3241 aaggagcgtt cgaatatgaa ctgcaattat cctgaccttt gtgcactgag cgccccgcgt
     3301 cttgccgcca tagcgctcag agatgaggac gtcatcaagg cattcacgcg ctttaacagt
     3361 ctaagggaaa tggaagatgc ccctgttgag gacatcatgg ctgcaggcct gacccgtgag
     3421 caggctgagt ccctcaaggc agccttagag cttggcaagc gcctggcgaa tgagcccagg
     3481 cccaagaaag caatattcgg agctgcggat atcgcagagc ttctaggacc tgagatgtcc
     3541 aagtatgacc gtgaacactt caaggttgtg atgctcgaca cgagaaacca ggtgatcgga
     3601 gttgagaccg tagccatagg gacactcaac atgtgccctg ctcatccccg tgaagtcttc
     3661 aagactgcca tactgaacag cgcggccgcg gtcatcatcg ctcacaatca tccaagtgga
     3721 tccccaagtc cctctcccga ggatatagcc ctatcaaagc gcatgcagcg ggcaggagcc
     3781 ttaatcggaa ttgatgtttt ggacagcatc gttatcggcg aggctccaac atacatgtcg
     3841 ctcaaacaga tgggagtcct atagcgcgca ccccaagcgc cggccgaacg agctggcgtt
     3901 cttgcatatt tatgcttact gtgaaaggag aaacaagcaa tgccaaggat caacgacgag
     3961 cacataatca ctatcgacgg taaaccctat gtcaagtatg gaggccttct ggagaccgca
     4021 cacagatctg ggctaaagga cttgcaggtc aagttgattc agtccccaag cagggagaac
     4081 ggctataccg ctgtgtgcga ggccacagca gtgattaaga ccaggagccg tgagaaggtc
     4141 tacgtcgaga tcggggatgc aagcccagcc tcgattggcg atcctaagct cgttccacat
     4201 gctatcagga tagcagccac gagggccaag gcaagggcct tgagggacgc actccacata
     4261 tccatctgct ctgtagagga gctgactata ggcaatggtt cagctccaga ggcttctgca
     4321 gccagctctc ccgctaaggc accacagccc gacaaagtca ccgagccaca gctcagagta
     4381 ttgcgcatgc aggctgaaca gcttgggctt agggacgagg attgcactag attcaacacc
     4441 atgactaaga aggaagcctc cgagactatc agcgcattca ataggcagct caatatggca
     4501 cgcgtcggtt gattgtaggt tagcctgagc atatgtgact ggaggaggtt catgtggact
     4561 tcctccagtg atgcattaca ggagtagtcg ggggtagacg tggagggtat tggggtaaat
     4621 gcacatctac gcccctacta tataaatacc actcaaaagc acttgattac acatgatgac
     4681 ggttactata ctctgcgaaa acctcgctct ccaggatgcc ttacaacgcg ttaaaatgca
     4741 tgcattagta tggttgtaag ggtatgactt gtttcgtgcc gcctgaaggc ccctgggcac
     4801 cttagggtga tgcatgtttt atgtggctac tgatatgagg taatatggtt gcgcggagtt
     4861 acgcaccagt tgccacgatc ccatcatcta ctgtgtcggc caccgggcaa taccacattt
     4921 cgagcaagtc aagccatgct tcccataact caggcacctc aaaaagtaat atcttttttg
     4981 ctttgtcagc ccttcccctg ttagtaatct gggcatacgt cttgagccca ttatgcactc
     5041 catcgtctgc gatggcatct cgggcggcct gctctacctc acataatcca ctggcctcca
     5101 tgagcctcat ctttgcgaaa gggtttcgcg gctcccaaca tcccgagaca aattcggcaa
     5161 cagtgggcct ccctttagta ggaagcctta tcttgacctc taggcgagtc agcccaataa
     5221 cgtcgatgtc gagttgcaca gctttgtcgt atatggtaat cctctccctg gagcgcttgc
     5281 caccaaagct gaaggaggtg ctgctgtgac tttgcctttg tctggcccac ttaatgctga
     5341 tagcatcagc caagtgcttg gcgtctgtgt gctccaaatc gagcttacat tctatgtatg
     5401 caatacgagc tgactcgata ggcatgtatt gggccactat ggcaccaaga tcctgtggtg
     5461 aaagcaagag cttcgaggga ttaatggtca tcgtcatgct tggcataatg ctgaactcag
     5521 gctcataaca caaggtaaag ctgccattcc catatggagt catatgccct cgctgttcca
     5581 tgatcctata cgaaccgcac ttcctatgtt tcttcttgct cttctcaacc ggacggcgga
     5641 caccagtgcg gcgataatac cttctgacta tattaacccc atctataggt ggcatctcag
     5701 attctctggg taatgagatt gggccacgat aatggatcct gatgagatca taggggatgg
     5761 gtccaactcc agtaaggatg ttgtcaataa aaagatgctc tttgatgatg tttgtaagga
     5821 ttgtatgcaa acagtgtcta ttactaatcc cgtcgagcac atgtctactc ccttcatacg
     5881 tattggtgtc gcgactatag gtgaagcaaa gtatttacaa atgggtcgtc tgggggttat
     5941 gatgaatccg acaacctcat gtgatgccat accagtccca tccagtattg catagttcgt
     6001 tgaatgcgtc aaccccataa gggatattat tattcacgga ggtggtgttg tgtcgatccc
     6061 tcgattatgg gagataacct gtgattgcct tgagcattac tgcagtatat cttatggcca
     6121 ggcaattgaa cttgagaaag tatcggacat aatcctcagt tcacccaaca tcaatgtggc
     6181 caatgtcaat gcagtagaca aggccatacg acagcttcgc actgcggcaa cgattctcaa
     6241 gaagatgatg gacagcaacc agcttgccca ggatgagttt gtaagcgttt ataaaaccct
     6301 acagaatata gatactgttg ccaacagact gctatttgga tccaaactga agaacgtccg
     6361 taacgccaag ggttacaatg ttacggaact ggccagcagt gtcggtgtgg atcggagcta
     6421 catttcaaaa cttgagaagg ctgtggcggg accacccgat atcgaggtta tagcacagct
     6481 atcttacgtt ttggccgttc caatgtctga attcgttgtg gatgccatag gtcatatcag
     6541 ctttgaaaga gctgataact ccaatattgt tgacccaaag aatgccatta tttcggacat
     6601 tgcacgggaa tgtgaagcaa tacccgagga gtatcttgtg atattgctgg cgcaggtcaa
     6661 agcgatatct caaacgtaca ggaaacgcaa attcgaaggt gtctggcgac gtctcgacca
     6721 gtgattatat cgtcacgtgc tgccttactg gtcccgacaa accaacatgc gtaggagagc
     6781 atctctatca ctgcgcagct tatcctgata tctaaaggcc aaggcaagct ctggacggac
     6841 atgcccttca aagcccgtgg gaacctctct gacaatgtca acatcaacgg cggataacat
     6901 tcttctgcga accatatgca cctcgttcat ggcccacata tgagcccagg tctcatcaca
     6961 gatgccctcg cgggccaatc catgcctgat gtcgtaatag gctattacct cgccagggca
     7021 aggatcccca taggctgttg cgtccagaat cttgcggccc tcatagctat gccataagcc
     7081 acacacttca tagaagtagc agccctcctt cttgcacggc aagaatccgt ctatggtcag
     7141 gccatgtttg agtgagttga ggga
//
LOCUS       DAOSYL010000089         7107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_8950_length_7107_cov_7.792967, whole genome shotgun sequence.
ACCESSION   DAOSYL010000089 DAOSYL010000000
VERSION     DAOSYL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_7107_cov_7.792967"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1018
                     /locus_tag="PLU15_10150"
     CDS             236..1018
                     /locus_tag="PLU15_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO31308.1"
                     /translation="MLKKDSFIPLYYQLKEELKEKIVSGEWEPETRIPSIRELCETYD
                     ISTTTAKQAISELVHEGLLYSVQGKGTFVSVPQWPMEYKETTYITDEMRLASRIRALG
                     HEFWAEVLTIDRVAASNLVSAYLAIEDDSEVLRIRRLKKVDGIPMLIETTFISPDVAI
                     GIEEADLSRSLFRILRDEYGIVLKRSDETFRPVFLGSLEADLLGQSVGALAMLNERIS
                     YAESPTPILYAKSLIRGDMCKTYVDLTNMRKVKEMVVNASSF"
     gene            1040..2371
                     /locus_tag="PLU15_10155"
     CDS             1040..2371
                     /locus_tag="PLU15_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HOO31309.1"
                     /translation="MKKAIVLTDGEHYPAVTHDAIEVLKGELDILAAVFIGGTEKIGS
                     DSDLARLGVPVVHEDDYLHAIAAAIDKYDPDEVVDLSDEPVVGYKERFEIASNVLAKG
                     VAYRGADFQFTPPVEAARVSRPSISVIGTGKRIGKTAVGGFVARTLAKKYNPVVVTMG
                     RGGPAEPELLSASEIEITPEYLLSVSKQGRHASSDHFEDLLTSRVTTIGCRRCGGGMS
                     GQTFVSNVDRGAELSEKIDADIVIFEGSGSSIPSVHTDARILVIGAHQPVEYMRSYLG
                     PYRILTSDLIVLTMCESPMVDQAKIDEMVEAIHEINAGCTVVKTIFRPRPIGDITGKR
                     IVLTLTTPAVMTDAISAYLEKTYGCEVVGVSSHLSNRPLLREDLARFETLKPDAIVSE
                     LKAAAVDVVTEWAVRKGLEIVYIDNEPIPTDASIDLEEEVLKVARKIKLHD"
     gene            2449..3429
                     /locus_tag="PLU15_10160"
     CDS             2449..3429
                     /locus_tag="PLU15_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO31310.1"
                     /translation="MKRGSVIVLALVLLLSISTGVFAESKVLLYTSVPLELATNFADQ
                     FMKANKGIKVEVFRAGSTDVVNKIWAEADAGGIRADVIWLADAASYYAFKDKGLLYPY
                     KSPEAARIPSAIKDPDGYFTGARMINMVIGINTNLVKKGSEPKTWDDLLGFGSRAAMA
                     DPLYSGSNFTVAAAFVQRDKGWSWYEKARKSGIQVLRGNSDVSRGLAAGEFAACMVLD
                     YMIYDLQKSGAPVDFVWPADGAVSIPSPVAIVAGSKNLEASKAFVDFTLSKKGQELLV
                     NDGVIPVRPDVAPPKGMPTADKIKMMPVPFQWAADNASAIRETFEKIMFK"
     gene            3552..5231
                     /locus_tag="PLU15_10165"
     CDS             3552..5231
                     /locus_tag="PLU15_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO31311.1"
                     /translation="MNTDTAVGRRSMDVRYVFMAIIGLFFLVVIVYPLSLVVVKSFSS
                     EAGFTFENYVKIFSESANLKAFWNSIWVSSLSTLICVAVGTILAMLVVRTDIPGKSWL
                     RMLLLLPYGIPPFIGAMAWAQLFGPVGYVSKLYMTLTGATSAPWNIYSAAGVVLVLAV
                     YQFPVVFITVSGALGKIDTSLEEAARMSGAGPFRTLIDVTMPLITPAITASALLAFVG
                     CISNFGIPALLGFRARFFVLTTKIYSALSIPDMPLATAMAVILAITSGIPLILERRLE
                     RGQSRYTVIAGKSVRPQTMSLGGWRIPVFIIVVLVSLFAGVVPLISMLLTSFLKYWGA
                     PLNLANMTTSHYKYILDLPMASRAFRNSLFLAVSAATITMAVGALVSYMSVRAKIKGA
                     KTLDFVGTIPYAVPCTMVAIAMILAWSRPPVMLYGTIWVILAAYLVRYMPFSIRTTNA
                     TLQQVHVSLEEAARVSGAGWTQTMRDIVMPLIKPGLIAGWILVFMPALRELTMSVLLW
                     SQGAETIGVVIYNMQDAGYTQIAAALSTIVLLVILAGNAIVRKLSKGEISM"
     gene            5254..6309
                     /locus_tag="PLU15_10170"
     CDS             5254..6309
                     /locus_tag="PLU15_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31312.1"
                     /translation="MEERIKVSNLVKRFGTFTAVHDVSFTVHENEFFSLLGPSGCGKT
                     TTLRCISGLEQPTEGEIHISGQLVTSPEKNVMVPPERRGIGMVFQNYAVWPHMTVEQN
                     MSYPLRLAKRPKEEINAKMAYLLDLLELEGLERRFPSELSGGQQQRVALGRALSTDPR
                     VMLLDEPLSNLDAKLREQMRFELKDIQRKTGISILYVTHDQVEAMAMSDRVAVMNSGK
                     VVQEGAPHDIYETPKDKFVADFIGTMNFVPCSVTGKTEKGIDIRLRGGAEFGLDGSSV
                     NKGDYILAIRPEDIILGTEQGVECEVDVRIFLGNVIEYRVRIGEDLLRVQTNSSMRFE
                     PGDRTRAFIQNGLVFPK"
     gene            6355..>7107
                     /locus_tag="PLU15_10175"
     CDS             6355..>7107
                     /locus_tag="PLU15_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase
                     (2,3-diphosphoglycerate-independent)"
                     /protein_id="HOO31313.1"
                     /translation="MQTTASLQQALRECYSRGEMDYWMTPLTRVVDGKPVGRIKNGDS
                     LFFCCRRGDRERQLTEAFADPLFDQFETEPMPDLSVVPLVQYHEKFAELPTIFPTLRP
                     EGTMGEALSQAGVAQTRIAESEKAAHVTFFFNGRRVAPYANEEWITVPSPHSSEFVSK
                     PATSTVEVALEAERSMAAANASGYSRHFILVNLAAGDIIGHLDDWNANVLCAEAVDQA
                     LSTICLAAAQHGYAVAVTADHGLLERFVNEDGS"
BASE COUNT         1686 a         1615 c         2005 g         1801 t
ORIGIN      
        1 ttatgcagct gctgtaactg cagatcatgg gctgctcgag aggtttgtga atgaaatggg
       61 catggcgtgg cagcagatgg ctgttgtaat taatggatgt ataaatggaa ggtcttaaaa
      121 gaggaatcgc ttaggaaatg tagaaataca gtaagcgata acacgttaac acacacactt
      181 ggtttagctg gcaatatgcc acaaggtcca ttacggtatg gaggctttcg tgccaatgct
      241 taagaaggac agttttattc ccctgtatta tcagcttaaa gaagagctca aggagaagat
      301 cgtctcgggc gaatgggagc ccgagacgcg catcccctca atacgcgagt tgtgcgaaac
      361 ctatgacata agcactacaa cagctaagca ggccatttcg gaattagttc atgagggcct
      421 gctctattct gtacaaggga agggaacttt tgtttcagtt ccgcaatggc ccatggagta
      481 caaggagacg acctatatca ccgacgaaat gcgtcttgcg tccagaattc gagctcttgg
      541 acacgaattc tgggcggagg ttcttactat agaccgggtt gcggcttcca atcttgtcag
      601 tgcgtatttg gccattgaag acgactcgga agtgttaagg atcagacgct tgaaaaaagt
      661 cgatggcatt ccaatgctaa ttgagacgac tttcatctcc cccgatgtgg ctatcggcat
      721 tgaagaggcc gatctatcaa gatcgctatt tcggatactc cgtgatgaat acggtattgt
      781 cctgaaaagg agcgacgaga ccttccggcc agtcttcctc ggtagcctag aagctgacct
      841 tcttggacaa tcagttggcg cattggcaat gctcaatgag cgcatttctt acgcggaatc
      901 gcccacacca attctatatg caaagtctct gatacgtggg gatatgtgca agacatatgt
      961 agatctcacc aacatgcgca aggtaaagga gatggttgtc aatgcatctt cattctagca
     1021 acaacggtgg tggagcatta tgaagaaagc tattgtactt acagacggtg agcattaccc
     1081 tgcggtaact catgatgcca tagaggtgct caagggggag ctcgacatat tggctgccgt
     1141 tttcattggc ggcactgaga agattggttc tgattctgat cttgccagac ttggagttcc
     1201 tgtagtgcat gaggatgact atctccatgc tatcgcagcc gctatagaca aatacgatcc
     1261 ggatgaggtt gttgatctta gcgacgagcc tgtagttggc tacaaggagc ggtttgagat
     1321 tgccagcaat gtgcttgcaa agggtgtcgc ttaccgtggc gcggatttcc agttcactcc
     1381 tcctgtagag gcggcgcgcg taagcaggcc gagtatctcg gtcataggaa ccgggaagag
     1441 aatcggaaag actgctgtcg gcggttttgt cgcgcggacg ctggcgaaga agtataaccc
     1501 cgtcgtggtt accatggggc ggggtggtcc tgcagagcct gagctgctca gcgcgtctga
     1561 gattgagatc acgcccgagt atttgttgtc cgttagcaaa caagggcgcc atgcatcgtc
     1621 tgaccatttc gaggatctgc ttacctcaag ggttacaacc atcggctgtc gacggtgtgg
     1681 aggcgggatg tcgggacaga cttttgtctc aaatgtggac agaggtgctg aactgtcgga
     1741 aaagatcgac gccgacatag tcatattcga aggcagcggt tcgtctatac cttcggtgca
     1801 cacggatgcg cgcatactag tcatcggtgc tcaccaaccc gtagagtata tgcgctctta
     1861 cctcgggcct tatcgtattc ttacctccga cttaattgtt cttacaatgt gtgagtctcc
     1921 aatggttgac caagctaaga ttgacgagat ggtagaagcc attcacgaga taaacgccgg
     1981 atgcacggtg gtgaagacca ttttcagacc tcgcccgatt ggcgatatta ccggtaagag
     2041 aatagtcctc actctaacta cgcctgcggt gatgacagat gccatatctg cttatcttga
     2101 gaagacttat ggatgtgagg ttgttggagt tagctcgcac ctttcgaaca ggccgctgct
     2161 ccgagaggac ctggcgcgtt ttgaaacgtt gaagcctgat gcaatcgtct ctgaactcaa
     2221 ggctgctgca gtagatgttg tgactgaatg ggcggtacgt aaggggcttg agatcgtgta
     2281 tatcgataat gagcctattc ctacagatgc ttctatcgat ctagaagaag aagtgctaaa
     2341 ggtggctcga aagatcaagt tgcatgacta gttgattcgc tggtcgtgcc cctgcgcgag
     2401 tgggggttaa ccaagtatac caccaatcaa aagagaggcg ggttgaacat gaaaaggggc
     2461 agtgtaatag tcctggcttt ggtattgttg ctgtcgatct ctactggtgt atttgctgag
     2521 tcgaaggtcc tgctttacac gtctgttcca ctggaactcg ctacgaactt cgcagaccaa
     2581 ttcatgaagg cgaataaagg gatcaaggtg gaagtttttc gagctggaag cactgatgtg
     2641 gtcaacaaga tatgggctga ggctgatgct ggcggtataa gggccgacgt aatatggctg
     2701 gctgatgccg caagctacta cgcattcaag gacaagggct tgctatatcc gtacaaatcg
     2761 ccggaagccg cccgcattcc ttcagcaatc aaggaccctg acggatactt tacaggcgct
     2821 cgcatgatta acatggtaat tggaataaac accaatttag tcaagaaagg atccgagcca
     2881 aagacttggg atgacttgct cgggtttggt tctcgcgccg caatggcgga ccccctctat
     2941 tctggttcca acttcactgt tgcagcggct tttgttcaaa gagacaaagg ttggagctgg
     3001 tacgagaaag cgcgaaaatc tggaatacag gttctcaggg gcaattcaga tgtttcgaga
     3061 ggtttggctg ctggcgaatt tgcagcctgc atggtgctgg actacatgat ttatgacctt
     3121 cagaagtcgg gagcgccggt tgatttcgtc tggcctgccg acggtgcggt ctcaattccg
     3181 agtccggtag cgatagtcgc tgggtcaaag aatcttgagg cctccaaggc atttgtagac
     3241 ttcactctct cgaagaaggg gcaggagcta ttggtcaatg atggtgtgat accagtgagg
     3301 ccagatgtag ctcctccgaa gggaatgcct acggcagata aaataaagat gatgcccgtt
     3361 ccattccaat gggcggctga taacgcgtct gctataaggg agaccttcga gaaaataatg
     3421 ttcaaatagg tgcgtgtcgt gaacaaacac cctgaccgcg gcggtgcgac tgaggccgca
     3481 ccgcctcttt ctggggagta cattactgta tgcagtgtac cccagcccat tgtaggaggg
     3541 ggatgattcg attgaatacg gacacagcag ttggacgacg atctatggat gtacgctatg
     3601 tgttcatggc catcattggg ctgttcttcc tggtcgtcat cgtgtaccct ctgtcccttg
     3661 ttgtagttaa gagtttcagt tctgaggcgg gttttacttt cgaaaactat gtgaaaatct
     3721 tctctgagtc agccaatctt aaagcctttt ggaattcgat ctgggtttcg tctctgtcta
     3781 ctctgatatg tgtggctgta ggcactatcc tggctatgct cgttgtccga acagatattc
     3841 cgggcaagtc gtggttgagg atgctgcttc tgctgccgta tggcataccg ccgttcattg
     3901 gtgcgatggc gtgggctcag ctttttggac cagtgggtta cgtatctaag ctatatatga
     3961 cccttacggg cgcgacatct gctccgtgga acatatacag tgctgccggg gtagtattag
     4021 tgctcgctgt atatcagttt cccgtcgttt tcataactgt gagtggggcg cttggcaaga
     4081 tcgatacgag cttagaagaa gccgcgagga tgtctggcgc aggtccgttt aggactctca
     4141 tagatgtaac tatgccactg atcacgcctg cgatcacagc tagcgcattg cttgcgtttg
     4201 tcggctgcat atccaacttc ggaattccgg ctcttcttgg gttcagggcc cggttcttcg
     4261 tgttgactac gaagatttac tccgctctgt ctataccgga tatgccattg gcgactgcga
     4321 tggcggttat cctggcgatt acatctggga ttccccttat tctcgagagg agactggagc
     4381 gtgggcaaag caggtataca gtgattgcgg gcaagagtgt caggcctcaa acaatgagtc
     4441 taggcggttg gcggatccct gtctttatca tcgttgtcct cgtatcgctt tttgcagggg
     4501 tagttccgct gatatcgatg cttttgactt cattcctcaa atactggggc gcaccgctga
     4561 accttgcgaa tatgactacg agtcattaca aatacatttt ggatctgccc atggcatcca
     4621 gggcatttag gaacagcttg ttcctggcgg tttccgctgc cacaatcacc atggccgttg
     4681 gagctttggt atcttacatg tctgtgcggg ccaagatcaa gggagccaag actctcgact
     4741 tcgtaggaac cataccttac gcggtgccat gtactatggt ggcaattgcc atgatcttgg
     4801 cctggtcgcg accccctgtg atgctttacg gcacgatatg ggtcatactt gcggcttacc
     4861 tagtgcggta catgccattt tcgattcgca ctaccaacgc gactcttcag caggtgcatg
     4921 tttcgcttga ggaagctgcg cgggtttccg gggcaggatg gactcagaca atgagagaca
     4981 ttgtgatgcc gttgatcaaa cctggcctca tagccgggtg gatcctggtt ttcatgccag
     5041 ctctgaggga gttgaccatg tccgttcttc tgtggtcaca gggcgctgag actataggag
     5101 tggttatata caacatgcaa gatgccgggt acacccagat agctgcggcg ttgtctacga
     5161 ttgttttgct tgtgatcttg gctggcaacg caatcgtgcg gaagctttca aaaggcgaaa
     5221 tcagtatgtg aacggtatgc ggaggtggca agggtggaag aacgaatcaa ggtttcaaac
     5281 ctagtcaagc ggtttgggac gtttacagca gttcacgatg tgagctttac agttcatgag
     5341 aacgagttct tctcgcttct ggggccctct ggatgcggga agacgacaac actccggtgc
     5401 atttcagggt tggagcagcc cactgagggc gagatacaca taagcggtca gttggtaact
     5461 tcgccggaga aaaacgtgat ggtgccgcct gagcgccggg gcataggaat ggtctttcaa
     5521 aactacgcag tatggccgca tatgacagta gaacagaaca tgtcctaccc ccttcggctg
     5581 gcgaaaaggc caaaagaaga gatcaatgct aaaatggctt acttgctaga cctacttgaa
     5641 ctcgagggct tggaaagaag gttcccatcg gaactctccg gtggacagca gcaacgcgtt
     5701 gcattgggga gagcgctttc taccgaccca agggttatgc tgcttgatga gcctctttcc
     5761 aatctcgatg cgaaactgcg agagcagatg cgctttgagc tcaaagacat tcagcgcaag
     5821 acgggaatat ccattctgta tgttactcat gaccaggttg aggcaatggc catgtcagac
     5881 cgggttgccg taatgaactc cggcaaagta gtgcaggagg gtgcgcctca tgacatatat
     5941 gaaacgccga aggacaagtt cgttgccgac ttcataggga ctatgaactt cgttccctgc
     6001 agtgtgactg gcaagactga aaaaggcatc gacattcgcc ttcgtggcgg ggcggagttt
     6061 ggtcttgacg gatcatcggt gaacaaagga gactatatcc tcgctattag gccggaggat
     6121 atcatcctgg ggaccgaaca aggggtcgaa tgcgaagtgg acgtaaggat attcctcgga
     6181 aatgtcatcg aataccgggt tcgaattggc gaggatcttc tcagggtcca gacgaattcc
     6241 tcaatgaggt ttgagcctgg cgaccgcacc cgtgcgttta tccagaacgg cttggttttc
     6301 cccaaataac attcgctgct tagagcagac aacgaagtgc aggagtgaca tggagtgcag
     6361 acaacagctt ctttgcagca ggctttgaga gagtgctaca gccggggaga aatggattac
     6421 tggatgactc cactgactcg cgtcgtcgat ggcaagcctg tgggcaggat aaagaatggc
     6481 gattctctat tcttttgttg caggcgtggg gaccgtgaac gacagctcac agaggccttc
     6541 gctgacccgc tatttgatca attcgaaacg gaacccatgc ctgatctgag cgttgttccg
     6601 ttggtgcaat atcatgagaa atttgctgag ttgcccacaa tctttccgac tcttcgtcct
     6661 gaaggaacca tgggtgaggc gctgagtcag gccggcgtgg cgcaaacgcg gattgccgag
     6721 tcggagaagg cggcgcatgt tacgttcttt ttcaatggca ggcgcgttgc accgtatgcc
     6781 aatgaagagt ggattactgt gccaagtccc cactcatcag aatttgtctc gaaaccggct
     6841 acgagcacgg tggaagttgc cctcgaagct gagcgctcga tggctgctgc gaacgccagt
     6901 ggttatagca ggcacttcat ccttgtgaac ctagcggcgg gggacatcat tgggcatctg
     6961 gatgattgga atgctaatgt gctctgtgct gaagcggtag atcaagcgct cagcaccata
     7021 tgcctagctg cggctcaaca tggttatgca gttgctgtaa ctgcagatca tgggctgctc
     7081 gagaggtttg tgaatgaaga tgggagc
//
LOCUS       DAOSYL010000090         6933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_9262_length_6933_cov_7.899680, whole genome shotgun sequence.
ACCESSION   DAOSYL010000090 DAOSYL010000000
VERSION     DAOSYL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9262_length_6933_cov_7.899680"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(622..1350)
                     /locus_tag="PLU15_10180"
     CDS             complement(622..1350)
                     /locus_tag="PLU15_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOO31314.1"
                     /translation="MSMFYDELYAMQYPPCPGGTIYIIRAGDTLYAIAQRFGTTVNAL
                     IAANPGINPLNLIIGQPICIPVPGPTPVPCPGFYYTIQPGDTYYLIALRYGTTVDALI
                     RANPGVNPNRLVIGQRICVPVAAPPPVAPRTCTVALSPRSGLAPNAGGVLWLRTDQFG
                     TAQVLVAGTNLPDPSTIGAETTYRAVFSWPGGTVSVPLTPATGQLGVWVGTAVQSLPS
                     VAFTSGAVDVFPGPVLGAFLRDCK"
     gene            complement(1788..4547)
                     /locus_tag="PLU15_10185"
     CDS             complement(1788..4547)
                     /locus_tag="PLU15_10185"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOO31315.1"
                     /translation="MRPIPDSVLECKMSLTPEALTIMESRYLLKDKEGNIIETPEEMC
                     LRVAREAARAGAQYEGTRESEPERVKLFYWLLVNHLFSPNSPTWMNAGTESSQNAACF
                     VLPVEDSLPQIFDAVRGAALIHQSGGGTGFDFSNLRPHGDVVRSTGGVASGPVSFMAV
                     FNAATETIKQGGKRRGANIGILRVDHPDIESFITCKVENTALSNFNISVGITDAFMAA
                     VEADTDYELINPRTKSAVGTKRARDVLNLIVDHAWKNGEPGVIFLDRIEEGNPTPSLG
                     RITATNPCGEQPLLPYEACNLGSVNLQKMYREAPLTDEELAAALGTGIHQPLLTGEFG
                     EGCRELITDEMRHVDWSLLGTVVRASADFLDSIIDVSEYPLPEIDKMVRTTRKIGLGF
                     MGLADLMIMLKVPYASEKAELLAELIAKFITGEARAMSRRMAAERGPFPAFSESVFAE
                     TGEPPLRNATVTTIAPTGTISMVANASSGCEPLFGVAFTKRNILGGKSLTQVNSLFLA
                     DAKQRGIYSDELMERIANNGGSLAGIDGIPSDMAKLYQTALEIPWEWHVRIQAATQRG
                     IDNAVSKTINLPNNATREDVGAAYRLAYSLGCKGITVYRTGSREVEVISSKKAAVASN
                     AQPEPLTPRGRKPFTVDSTLWGKLQPIDRPETLCGITKRIATPSGTLWATLNMYDGKP
                     FEIFCQIGRAGTDIYAFTESIARLISLALRAGIDPSEIVRQLRGIGGSGQVGFGPNKV
                     LSVPDAIGRMLEQCIQSRYAGIEWATPPSRGVAGQTAPEDSWEAAGIDISPDEATVQG
                     RLLFIDEEEDTAVDHGDAAEDGASMDFEVGDLSPEPARQADNFEEEDPSASNNGVESS
                     ETNSHADGMLAMSDDFGGHMGDPDYTSSGHRVTRHDLCPQCGNYSLVYASGCSECRLC
                     GYSAC"
     gene            complement(4920..6353)
                     /locus_tag="PLU15_10190"
     CDS             complement(4920..6353)
                     /locus_tag="PLU15_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOO31316.1"
                     /translation="MLRLRHNVIWILASAVLLIGIASTFLRPTAIGAQKGADNSMELT
                     IYNSDLALVKETRCLEIPEGLSTVLFPNISARINPATVSFVSLTSPEEVRVREQNYEY
                     DLVEDTKLMQRFVGEKIQVTTKGGRAYTGYLLSGAKGSAPSNIIMSEGPNGTGQVYTV
                     QLSDVQSVEYPSLPEGLITRPTLAWLVSNNSITRNHDCLVTYLTGGLSWAADYVAIIG
                     PNDDNLDLRGWVTVTNESGATFKDAKLKLVAGDVHQVQEEMYDVVRATAKVLDAGTGP
                     TFEEKPFFEYHLYTLQFPTTLKENQIKQIELLAASSIPARKLLVYDGARQGTKVTTML
                     EFWNKTENNLGMPLPKGVIRVSKMDTDGSLEFIGEDRIDHTPKDEKVRIELGSAFDVV
                     GERSVMGTKRLSDRVNEQTFKIEVRNHKEEPVEVTVLEHMMGWAQWEIVSSSTKWNKV
                     DANTIEFPTKAPAGGSTTITYTVRYTY"
     gene            complement(6597..>6933)
                     /locus_tag="PLU15_10195"
     CDS             complement(6597..>6933)
                     /locus_tag="PLU15_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31317.1"
                     /translation="VSALTDGIITPEQVDAISRGKWDEARNGMSPALAQRLVEMGLTE
                     EEAQALLAEEWDALPELAQRRLAMALSEEIGVSPELIESIMNRDWKSARSYAELEVAE
                     LLLSRFLNM"
BASE COUNT         1453 a         1822 c         1875 g         1783 t
ORIGIN      
        1 cacccgcgcc cgaaaagcga ctaaataata gacatattcc tttgaatgca tagactgctg
       61 gagcgctaat atggatctca tggtcgggag cgtgtgcgcg tcgctttcgt ggttgtaaag
      121 aagacggttg cccgcaagcg tcgaaccggg atttcgatct accctggcct tgccccgagg
      181 atcatatccc ggcgcggcta agtgctgcgg gcgtctatgg ctaaggccaa gatgcgtatg
      241 ttgtagatgt acaacaccta gcatacatca tatggcaggc taaccgatag cgcaaaaact
      301 tatggggagt tacttagtcg tgaatggtct accggatcgc tttcttcgca ggagagtatg
      361 ggtgttgtcg tcggccaatt tccacggccg gttttcatgg gtatctttgc tgtcttcacg
      421 ggcgatggag atagttctgg caaacgacat tctgttccag acattgtaga ggttgttctt
      481 caagttcaac ttgaacgatt tcgacgcttc ttcgttcccc tgtcgaccta tccgaatctt
      541 ttgagctgcg aaatggcgtg cggcaagggg gtcggttgcc ggcccccatt gccacatgcc
      601 atacgatttc gtgtttcttc cttacttgca gtccctcaag aatgccccga gaactggacc
      661 agggaagaca tctacagctc cgcttgtgaa cgccaccgag ggcaagctct gtacggcagt
      721 tcctacccac acgccgagct gacctgttgc tggggtgagc gggacgctta cagtgccgcc
      781 tggccagctg aatacggcgc gataggtagt ttcggcgcct atcgtcgacg gatccggcag
      841 atttgttcct gcgacaagga cttgtgccgt cccgaattgg tccgtcctga gccatagcac
      901 tcctccggcg ttcggcgcta gtccgctgcg aggggagagt gccaccgtgc aggtgcgggg
      961 cgccactggc ggaggcgctg caacaggcac gcatatcctc tgccctatca caagcctgtt
     1021 aggattgacg ccggggttag ctctaatgag ggcgtcaaca gtagtgccgt atcttagtgc
     1081 gatcaagtag taggtgtctc cgggctggat tgtgtagtaa aaccctggac atggtacagg
     1141 cgttggaccc gggaccggta tgcatattgg ttggccaatg atgaggttca aggggtttat
     1201 tcccgggttt gcggcaatga gcgcgttcac ggtggttccg aagcgttgtg cgattgcgta
     1261 tagagtatcg cctgctctga taatgtaaat ggttccgcca gggcatggag gatactgcat
     1321 ggcgtagagt tcgtcataga acattgacat atgccatcac tcccgtgttg gtctgctaca
     1381 aagtatgcag aagggccaag ttcggcattg tgcttcgggc aatgatggac caaaggcatg
     1441 gtcgggaagg ctctttcagt tggttttttg tctaggaata ttccgggcgt ggatggatgc
     1501 aaggtctgct cagggcgtgg ccgggtgacc gagccaacac gatttccttg gcgcaagcaa
     1561 gtcgccaagg aaatcgagct ttagcttgac atgatcagta aagtccgcgg cagcctggat
     1621 cgctaagctt tgtggaacga aaacgggttt atgggaaggg ccagacaggt ttggcaagcg
     1681 gacgacgctg cgcaaaggag atccggtctc cgaggcagcc accgcccgct cgtggacatc
     1741 catgtcagct agtaggatct aatatagaat gaagagccct acatctgtta acaggcacta
     1801 tatccgcaca gcctacattc cgagcagccg gacgcatata ccagcgagta gtttccacat
     1861 tggggacata gatcatgcct tgttacgcga tgcccggatg aagtgtagtc tgggtcgccc
     1921 atgtgtcctc cgaaatcgtc agacatggca agcattccat ctgcatggct gttggtctcg
     1981 gatgattcta caccattgtt gctggctgag ggatcctctt cttcaaagtt atccgcttgc
     2041 cgcgctggtt ctggagagag gtctccgact tcaaaatcca tggatgctcc gtcttccgca
     2101 gcatccccat ggtcaactgc ggtatcctct tcctcgtcta tgaagaggag ccgcccttgg
     2161 accgtggctt catcgggcga gatatcgatt cccgctgctt cccaactgtc ttcgggagct
     2221 gtttgccctg caacgccgcg gcttgggggc gtggcccatt caatgccggc gtaccgcgac
     2281 tgaatgcact gctccaacat tcgacctatg gcgtccggta ccgagagcac tttgttggga
     2341 ccgaatccca cctgacctga accgcctatg cctctgagct ggcgcactat ttcagatggg
     2401 tctatccctg cacggagagc taacgagatt agcctggcga tactctcagt gaacgcatat
     2461 atgtcggtgc cggccctgcc tatctggcag aatatctcaa atggcttgcc gtcgtacatg
     2521 tttagcgtgg cccagagtgt tccggatggt gtagcgattc tcttcgttat gccgcacagt
     2581 gtttcgggcc tgtcgatggg ttgaagcttc ccccagaggg tggaatcgac agtgaaaggc
     2641 ttgcgtcctc taggcgtcaa gggctccggc tgagcgttcg aagcaactgc agcctttttg
     2701 gacgagatta cctctacctc gcgactcccg gtccgatata ctgttatgcc tttgcagccg
     2761 agggagtagg cgagccggta agctgcgcca acatcctcgc gggtggcgtt gttgggcaag
     2821 ttaatagtct tcgataccgc attgtctatg cctcgttgtg ttgcagcctg aatccgcaca
     2881 tgccactccc atgggatttc caatgctgtt tggtatagct ttgccatatc ggaggggatg
     2941 ccatcgatcc ctgccagcga accgccgttg ttagcaatgc gttccatcag ctcgtccgag
     3001 tatatgccgc gctgtttggc gtcggcaaga aacagcgagt tgacttgagt gagagacttg
     3061 ccgcctagta tgtttctctt ggtgaatgcc acgccgaaca agggctcgca ccctgaagaa
     3121 gcgtttgcta ccatgcttat tgtgcccgtt ggcgcaatcg tagtgacagt ggcattacga
     3181 agcgggggct ctccggtttc ggcgaatacg ctttcggaaa acgctgggaa tggccctcgt
     3241 tctgcagcca tccgcctcga catcgcccgt gcttcacccg tgatgaactt ggcaatgagc
     3301 tcggcaagaa gttctgcttt ttccgatgca tacggtactt tgagcatgat cataagatct
     3361 gcgaggccca tgaaaccaag accgattttt ctggttgtcc ttaccatctt gtcgatctct
     3421 gggaggggat actcgcttac gtctattata gaatccagaa aatccgctga agcgcgaacc
     3481 acagtgccaa ggagactcca gtcaacatgg cgcatctcat ctgtgattaa ctcgcggcag
     3541 ccctctccga actcgcctgt gagcaatggt tgatgaattc ctgtgccaag ggcagcagca
     3601 agctcctcat cggtcagtgg cgcctcgcgg tacattttct gcaagttcac gctgccaagg
     3661 ttgcatgcct cgtaaggcag caaaggctgt tccccacagg gattcgtggc ggtgatgcgg
     3721 cctagcgaag gggtggggtt gccttcttca attcgatcta ggaatatcac gcctggctct
     3781 ccattcttcc acgcgtggtc cacaattaag ttgaggacgt cgcgagccct cttcgttccc
     3841 actgccgact tcgtcctggg attgatgagt tcgtaatcgg tgtcggcttc cacggcggcc
     3901 atgaaggcgt ctgtgatccc tacagaaata ttgaaattgg agagagcggt attctccact
     3961 ttgcacgtta tgaaggattc gatatcggga tgatccactc ggagtatccc tatgtttgcg
     4021 cctcggcgtt ttccgccttg cttgatggtt tcagtggcgg cattgaacac agccatgaaa
     4081 gagaccggac ctgaggcaac tcctccggtc gatcttacga catcgccatg agggcgtaaa
     4141 ttagaaaagt caaatcctgt tccgcctccg gattgatgta tcagcgcggc accgcggact
     4201 gcatcaaata tctggggcag agaatcttct acgggtagga cgaagcatgc ggcattttgc
     4261 gagctttcag tgccggcatt catccaggta ggagaatttg gcgaaaacaa atgattgacc
     4321 agaagccagt aaaagagctt cactcgttct ggttcagatt cacgcgtgcc ctcatattga
     4381 gcaccagccc ttgccgcttc tcttgctact ctgaggcaca tttcctcagg ggtttcgatt
     4441 atgttccctt ccttgtcttt taacagatat cggctttcca tgatggtcag cgcttcgggg
     4501 gtaagagaca ttttgcattc aaggacagag tccgggatgg gcctcatcta tcttcctcct
     4561 gttcgagagg gtctttacga tcctagaact cctctctagg attgactcag ttttcaacac
     4621 cgccaacctg attcctgcat gcgaaatgtg atatagaata tcgaaatttc catgccccag
     4681 ctctccggga tatggaagaa tcggggtgtg ttagccagta gagtcatcag catgcatcaa
     4741 tatgataacc cataaagcga acgtaaagtg tgccaaggac acaagaaaaa agagagtgaa
     4801 caaaggaagc ggattgaagt tgactggatt ttcgctttcg ccggcttcga cagagaaaac
     4861 aaggaggatc gcccgaaagc gaccctcgaa ttcagcttac gaaccggtct gatcctggac
     4921 tagtaggtat accttaccgt gtatgttatc gtggttgatc cgccagccgg cgcctttgtg
     4981 gggaactcga tcgtgttggc gtctactttg ttccacttgg tgctggagct gacaatctcc
     5041 cactgggccc agcccatcat gtgttcgaga acagtgactt ccactggctc ctccttgtgg
     5101 ttgcgcactt cgattttgaa tgtctgctcg ttgactctgt cagaaagcct ctttgtgccc
     5161 attacagagc gctctcccac gacgtcgaag gctgatccca gttcgattct gactttctcg
     5221 tcctttggag tgtggtcgat cctgtcttcg ccgatgaatt ccaggctacc gtcggtatcc
     5281 atcttgctga cccttatcac gccctttgga aggggcatcc cgaggttgtt ttctgttttg
     5341 ttccaaaatt ccagcatcgt ggtgaccttt gttccttgcc ttgcaccgtc gtataccagc
     5401 aattttctag ccggaatcga gcttgctgcc agaagctcta tctgtttgat ctggttttcc
     5461 tttagtgttg ttggaaactg aagagtgtaa aggtgatact cgaagaacgg cttctcctca
     5521 aatgtaggcc ccgttccagc gtcaaggact tttgctgttg cccttactac gtcgtacatc
     5581 tcctcttgca cctggtgcac gtctccggcc acgagtttga gctttgcatc cttaaatgtg
     5641 gctccggatt cgttagtcac agtgacccaa ccgcgaaggt ccagattatc gtcgtttggt
     5701 ccgatgatag ccacgtagtc tgctgcccaa gaaagtccac cagtgaggta cgttaccaag
     5761 cagtcatggt ttcgggttat ggagttgtta gacaccagcc acgccagggt gggacgagta
     5821 atcaggcctt ccggaaggct gggatactct actgactgaa catcgcttag ttgcacggtg
     5881 tagacttggc ctgtgccgtt tggaccttcc gacatgatga tgttggaagg ggcggatcct
     5941 tttgccccgc tcagcaaata tcctgtgtag gcgcgcccgc ctttcgtggt aacttgaatc
     6001 ttctcaccga cgaatctctg catgagtttt gtgtcttcaa caagatcata ttcgtagttc
     6061 tgctcgcgca ctctgacttc ctctggagat gtgagagaga cgaaacttac agtggccggg
     6121 tttatccggg ccgatatatt aggaaacaac accgtggaaa ggccctctgg gatctcgagg
     6181 cacctcgtct cttttacgag cgcgaggtct gaattgtata tggttagttc catggagtta
     6241 tcggctccct tctgagcgcc aatcgccgtg ggccgtagaa aagtcgacgc aattccgatt
     6301 aacaggactg ccgatgccag tatccagatg acgttgtgtc gtaggcgaag cattacagca
     6361 ccctccaata ctagttattg cctcagtttc ggatgtcaat cttcttgtct cttacttttg
     6421 attccttcaa gatgagaagg ccatatcctg aaaccagcgt attcatacac aacctgcttt
     6481 cactctgaag agacgccgcc gccgcgctgt ttgttccaac gcggcggcga aacatgttgc
     6541 ctgctgccta gcgacgagaa caagtctgtt ggcagccagg ttcgggtggc gcacctttac
     6601 atgttcaaga atcttgagag cagaagctct gctacctcga gctcagcata gctgcgcgcg
     6661 gatttccaat ctcgattcat gatggactct atgagctcag gtgatactcc aatctcttcg
     6721 gacagagcca ttgccagacg tctctgagcc agttcaggca gggcgtccca ctcttcggcg
     6781 aggagagcct gggcttcctc ttcggtcaag cccatttcta cgagcctctg ggccagtgct
     6841 gggctcattc cgttccgggc ctcatcccat ttcccacggg atatggcatc cacctgttct
     6901 ggcgtgatga tgccgtcagt gagggcgctc act
//
LOCUS       DAOSYL010000091         6845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_9434_length_6845_cov_6.337261, whole genome shotgun sequence.
ACCESSION   DAOSYL010000091 DAOSYL010000000
VERSION     DAOSYL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9434_length_6845_cov_6.337261"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLU15_10200"
     CDS             complement(<1..510)
                     /locus_tag="PLU15_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31318.1"
                     /translation="MHTKARRRLVPIVALCLLLMAVGSAAASSADDGYADGEVFLSIS
                     PYMEADKEFLVENTAQIADVESKVNAHRFADQDCMFTYRLNLEGLILPAELRISTVGA
                     QYKVSVSNDGETFAVIAVETERVKDESNKTRIEYRITQDDLDAGTLFVRFEDSFPEDG
                     WGASFRRGPL"
     gene            complement(558..3257)
                     /locus_tag="PLU15_10205"
     CDS             complement(558..3257)
                     /locus_tag="PLU15_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOO31319.1"
                     /translation="MATFLRGMVAHGGAIIPAIVENASFAVKMIGEIYVICSQAAPFV
                     KAVSVIVAIAILLGSVSGTCSAATQMDGVSEVSHRFPCIARPERLYVISKSAMTDAEL
                     LMVSSLQGIVAQTRPEIYIDSGSAYRGWLELIRAEHGIPMQYHDEAEWFVDHYKDIFK
                     ENGYVLADLKSECVNVATTVSGLLEAPIVDQSLLPLMYARGFEMAIDVRDKDECWAFE
                     NYKDKLNPNILFRQDPLKLQLRDYAIACKGFVFYQPDNDEFVATVYDWAQEDAPVLGW
                     GPGFEDDHVAKASEHNHMTIPADWAMNLSALAGIGHRSLQQRNHVANIRFEPDVHYVT
                     FVMSDGDNVQWLLGDMAVKPHHYGSAVRGKFPMGFTISPSLIGLAPAAMGWYYDTAKL
                     DYFVAGVSGTGYIYPSKYPRLDSFAFELDQYLAMSDLSIVSILDQCPMGSKEFHDLAQ
                     AYAGLSNVIGGIYLTGPKYAGGNGNIEWVDGKPFVSIRESLWLADPAEIAARINLLPA
                     DPESPEGYTIVNVHPWSHTMADVKAVVDNLEPHVRVVNPEEFFLQLIFNQDGRFPTTR
                     RSVEANITNPINREAVRGIIVVNGNASSAAPIRSVQVAVGDGPWREAEPVEEDFRCWQ
                     ASVDTGNVEDGLHALRVRVTTEDGEVYYCSEEIVVRNDSESGTVVGTVMRAGLPVEKA
                     EVELMGRHAVTDSHGSFEFEFVPSGIVEISVAAKGSDPLTTSVEVVTACTSAIVLNLP
                     VEADLRTAWTFDRDEDGWKGGAVSGEPAAKGKAKWDGSIGNGPGSLRLDGTDSDSLDG
                     KANSWYFNVVKLPDDVGTISFDTRGIDAKGKVGGALRLVLVDDDGCAHVLLPWQATNS
                     EEWVTKTADISAFAGQIVAIRFELDAVEVGVKGYRYIDNIVIK"
     gene            complement(3260..4537)
                     /locus_tag="PLU15_10210"
     CDS             complement(3260..4537)
                     /locus_tag="PLU15_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO31320.1"
                     /translation="MGANSAKDRRFDRVRRWMLLAVIIVSLLGCSSHGLAAKTRLIIW
                     WPQGSTEKAVLQAAIADYAKVNPSVEIVPQWQPDSDDTQKLKIAYVGGNAPDIVRIDH
                     VWVQALGYKRMFTDLVPYGANSVKNMFFDPTWQANTYKDKVFALPFDANVICLMYNNT
                     LLKSTGTTVPTTYDELIAVGKANTRDINNDGKIDKWGYTIPVSAGGSGWLQFQWLTWL
                     WRMGGEVLNEDWTRAVFNSPEGVKSLEMMIDVVKKHKIAPDGAYYEGDFYNGNVAMID
                     MGPWSMSGRDPKVFGFAPLPVLNPRVGEATGLGLYSLAITSSCKNPKAAYDFVKFVTT
                     TPKYSAMWSKGTYLMPSLKTAYSDPLYRTGYWSVFMSQLPKAKMRPGTPAWPEIAGYI
                     SDAIQSVLAEKATAAEALNKAAELSNKALSKVK"
     gene            5047..6678
                     /locus_tag="PLU15_10215"
     CDS             5047..6678
                     /locus_tag="PLU15_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOO31321.1"
                     /translation="MDRKHGFNRHLCFIVLCLLLATASTAAVGASPIKVDFYVIVPDR
                     TPTGDIVYIGSEAANQRDAASVAMRHIGNNVWTTTLEIAEGQILDYKYTRGTWETVEK
                     DSTGADIETRSVVALDLGGGQLLVIDYVPLWADELDRADVTPITALQSIAPAYEESDT
                     LEAPIREIVYLGSQNFYDGLPVLYLHEPVKPAKNAILLINDGTGLSHRTLARICAGGP
                     NYRLPSEQMPIVGLANTHSRGSLVTDSAAAGTQLATGYWVNNRTISVDPDGVPLKTIL
                     ELAKELSKSTGIITTDSVTGATPASFGSHAGDRGDDPAISTGIFDNRVDVVMGGGRKH
                     FVPGTDNGARIDGRNLLKELQDEGFTVVDNVDDLKADNNLRAWALLNSGSLTGAASRN
                     YTLGDLTEEAIRRLSQNENGFFLMVEGDQIDGGGHNNDGYRVVTEMLDFETAVQVAVD
                     FASGRDDTLVVVVADHETGGLGITSGSAYGTSAEIGWVHTGHTGSPVAVYSFGPNSVL
                     LGSHMYLANIPKIISLGWGVTDFGPERELTGPGAF"
BASE COUNT         1684 a         1921 c         1640 g         1600 t
ORIGIN      
        1 cagcgggccg cgccgaaagg aagcccccca tccatcttca ggaaagctgt cttcgaacct
       61 cacaaaaagt gtgccagcgt ccaaatcgtc ctgggttatc ctgtattcga tcctggtctt
      121 gttcgattca tcctttacac gctcagtctc gaccgcaatt acggcaaatg tctccccgtc
      181 attcgatact gagaccttat actgcgctcc cacagtgctt atccgcagct cagcggggag
      241 aattaagccc tcaagattga gcctgtatgt gaacatgcaa tcttggtcag cgaacctgtg
      301 agcgttcacc ttgctttcca cgtcggcaat ctgagcagta ttttcgacca ggaactcctt
      361 gtctgcctcc atatacggac tgatactgag aaacacttct ccatctgcat atccatcatc
      421 agcggaagat gcagcagcgc tccccactgc catcaacaag aggcataggg caacaatggg
      481 caccagccgg cgccgtgctt tcgtatgcaa ccgagctccc tccatgtagt cacccacggt
      541 tttcgtgaga tcattgtcta cttgataact atattgtcta tgtatctgta tcccttaacg
      601 cctacctcaa cagcatcgag ttcaaatcgg atcgccacta tctgaccggc aaatgccgaa
      661 atatccgctg ttttcgtcac ccattcctct gaattcgtgg cctgccacgg caagagcaca
      721 tgggcacatc catcatcatc gacgagcaca agacggaggg ctccgcctac cttgcctttt
      781 gcatcgatgc cccgcgtatc aaaggagata gtcccgacat catccggcag cttcacaacg
      841 ttaaagtacc agctgtttgc cttgccatcc agggaatctg aatcggttcc atccagccgt
      901 agacttccag gtccatttcc aatagaaccg tcccatttcg ccttgccctt tgcagcaggt
      961 tcaccggata cggctccgcc tttccaacca tcctcatctc gatcgaatgt ccatgccgta
     1021 cgcaggtcag cctcgactgg caggttgaga acgatagcgc tagtgcacgc agtcacaacc
     1081 tctaccgaag ttgttaatgg atcagaccct ttggctgcca cacttatttc gacaattcct
     1141 gacggcacga actcaaattc aaaactaccg tgagaatcgg taactgcgtg acgccccatc
     1201 aattcaactt cagccttctc gactgggagg cccgcacgca tcacagtccc gaccacggta
     1261 ccgctttcgc tgtcgttccg gaccacaatc tcctcagagc aatagtagac ctcgccgtct
     1321 tctgttgtaa ccctaactct caaggcatgc agcccatctt ccacattgcc ggtgtctaca
     1381 gatgcctgcc agcaacggaa atcttcctcc acaggctcag cttctcgcca tgggccatca
     1441 cccaccgcta cctgcacact tcttatcggc gccgcactgg aagcattccc gttcacaacg
     1501 atgatgcccc tgacagcctc tcgatttatg gggttggtga tgttagcttc tacgctccgg
     1561 cgcgtcgtgg gaaacctccc gtcttggttg aatatgagct gcaagaaaaa ctcctcggga
     1621 ttcacaacgc gcacgtgagg ctccaaattg tccacgacgg ccttgacgtc ggccatagta
     1681 tggctccacg gatgcacgtt cacgatagtg tatccctcgg ggctttctgg atcggcaggg
     1741 agcaagttta tccttgctgc aatctccgca ggatcggcca accataagga ttcccttatg
     1801 gaaacgaaag gcttgccgtc aacccattcg atgttgccgt ttccacccgc gtatttcggc
     1861 ccggtgaggt atatgccccc aatcacgtta gaaaggcccg cataggcttg agcaagatcg
     1921 tggaattcct tcgatcccat aggacactgg tccaatatgc tcacaattga aaggtcggac
     1981 atggcaaggt attggtcgag ttcgaaagcg aaggagtcga gcctaggata cttagaagga
     2041 tatatgtatc cagtgccaga cacgcctgcc acgaaatagt cgagctttgc ggtgtcatag
     2101 taccatccca ttgccgcagg ggccagtccg attaggctag gagaaatcgt gaaacccatt
     2161 gggaatttcc cccgcacagc gcttccatag tgatgcggct tgactgccat gtcgcccaaa
     2221 agccactgca cattgtcgcc atcgctcatc acgaatgtga cgtagtgaac atctggctcg
     2281 aacctgatat tggccacgtg attgcgttgt tgaaggctcc tgtgtccgat cccggcaaga
     2341 gcgctgagat tcatggccca atcagcggga atagtcatgt gattgtgctc ggacgcttta
     2401 gccacgtgat cgtcctcaaa tcctggaccc catcccaaca caggggcatc ttcctgagcc
     2461 cagtcataga ccgttgcaac gaattcatca ttgtcaggtt gatagaacac aaagcccttg
     2521 caggctattg cgtaatctct aagctgtagt ttgagagggt cctgccggaa gagaatgttt
     2581 ggattcaact tatccttgta gttttcaaac gcccagcact catccttgtc ccgcacatcg
     2641 atcgccatct cgaaacctcg cgcatacatg agtggaagca aggattgatc cacaatcggt
     2701 gcctccagga ggcccgacac ggttgtggct acattgacgc attccgattt gagatccgca
     2761 agaacataac cattctcttt gaaaatgtcc ttgtagtggt ctacaaacca ttccgcctca
     2821 tcatggtact gcatagggat gccatgttcg gcgcgaatca attccagcca accgcgataa
     2881 gccgatccgc tgtcgatgta tatttccggc ctagtttggg ccactattcc ttgcaatgat
     2941 gagaccatca gaagctcagc atcggtcatc gcagacttgg aaatcacgta cagacgttca
     3001 ggccgggcaa tacacggaaa acgatggctc acttcgctaa caccatccat ttgcgtcgct
     3061 gcagaacacg tgcctgaaac tgaacccaga agaatagcaa tcgcaacgat gacagaaact
     3121 gcttttacaa acggggctgc ttggctgcag atcacatata tctcccctat catcttcact
     3181 gcaaagctgg cgttctcaac aattgcgggg atgatggccc ctccatgagc caccatgccc
     3241 cgcagaaacg tagccaaggc tacttgactt tagacagagc cttattgctc aactcagccg
     3301 ccttgtttag tgcctcggca gcagtggcct tttcggccag aacactttgt atcgcatccg
     3361 agatatatcc cgctatttca ggccaggcag gcgtacccgg acgcatctta gccttgggaa
     3421 gctggctcat gaacacagac caatatccgg tcctatatag cgggtcgctg taagccgtct
     3481 tcaggcttgg catcaagtat gtgcccttgg accacatcgc cgagtatttt ggcgtcgtcg
     3541 tcacgaactt gacaaaatcg tacgccgcct tcggattctt gcagctgctg gtaatcgcaa
     3601 gcgaatagag cccgagtcca gtagcctcgc ccacacgcgg attcaaaacg ggcagaggag
     3661 caaagccaaa aaccttggga tctctgccgc tcatggacca cggtcccata tcgatcatgg
     3721 caacgttgcc attgtaaaaa tcgccttcgt aatatgcgcc atcaggagca attttgtgct
     3781 ttttgactac gtctatcatc atttccagag acttcacgcc ctcagggctg ttgaaaacgg
     3841 cgcgagtcca gtcttcattg agaacctctc cgcccatcct ccatagccat gtgagccact
     3901 ggaactggag ccatccactg cctccggcac ttaccggaat ggtgtatccc catttatcta
     3961 tcttgccgtc attgttgata tctctggtat tggcttttcc gacagctatc agctcgtcgt
     4021 aggtagttgg aacggtggtg ccggttgatt tgagaagagt attgttgtac atcaggcaaa
     4081 tcacgtttgc gtcgaaaggc agggcgaaca ctttgtcctt gtatgtattc gcttgccagg
     4141 tgggatcgaa aaacatgttc ttcactgaat ttgcgccata gggaactagg tcggtgaaca
     4201 tacgcttgta accgagagcc tggacccaca cgtggtcaat cctgacgatg tcaggcgcgt
     4261 tcccgcccac ataggctatc ttcagctttt gcgtatcatc ggagtcgggt tgccactgcg
     4321 gaacgatctc gacagaggga ttgaccttgg catagtcggc tattgccgct tgcaataccg
     4381 ccttctctgt ggagccctgc ggccaccata taataagtct agtctttgca gccaatccat
     4441 ggcttgaaca gcccaatagt gaaactatga tcacagccaa caacatccat cgtctgaccc
     4501 ggtcaaaacg gcgatcctta gcgctgttag cgcccatcag agagtcaccc ccatgtttta
     4561 gtagcacatg cacaggttag tgcagcaatc aaaagtttgt acggacattc ctcatcgcat
     4621 caaaccttta caaccataat tacgggacaa cacttggcgg agtaatttgt cgccaatccc
     4681 gcccccgttt tctccagttc gccgcctgtt cacggacagt cggcggcatt gtaatgtacg
     4741 gacattcatt gttcctattc tacgcgccca cgtgaactcc ttcccgctcc tctttaaaac
     4801 ttaaggcgca tcaacgccac agttacctgg cacgtatagg atatccgcag taacgccagg
     4861 gagtcttcga ctaaaatgat cgtccactct ttgaaggatt atgctctgaa acgttgaaat
     4921 agataacggc atatgtacgg acatttgctt tatctctgtc aagccaaact gcgataaagc
     4981 atgctttcgc ctgtaatacc aacaaggcta tacgtgtagc cggaacaata aaaggaggat
     5041 cttcttgtgg acagaaaaca tggtttcaac cggcacttgt gcttcatcgt cctctgtctg
     5101 ttgctggcaa cagctagcac agcagctgta ggtgcttctc cgatcaaagt agacttttac
     5161 gtaatagttc cggatcgcac tccaactggt gacatagtgt acataggaag cgaggcagct
     5221 aaccaacgcg atgccgcaag cgtcgccatg agacatattg gcaacaacgt atggacaacg
     5281 acactggaaa tagccgaagg ccagatatta gactataagt acacccgagg aacttgggaa
     5341 accgtagaaa aagattcaac cggggcggac atagagacta gatccgttgt ggcgctcgat
     5401 ctgggcggtg ggcaattgct cgtcatcgac tacgtcccat tatgggccga tgagctggat
     5461 cgggcagatg tgacacctat aacagccctt caaagcattg cccctgcata tgaggaatct
     5521 gacaccttgg aggccccaat ccgagaaatc gtttatctcg gaagccaaaa cttctatgac
     5581 ggacttccgg ttctgtatct gcatgaacca gtgaaaccag ccaagaatgc gattcttctc
     5641 atcaacgatg gcactggcct gagccaccga accctggccc gcatctgtgc cggaggcccc
     5701 aactaccgcc ttccaagcga acagatgccc attgttggtc ttgcaaacac ccattcgcgt
     5761 ggttcgcttg taacagattc cgcagcagcc ggcactcaac tggccacagg gtactgggta
     5821 aacaatagga ccatcagcgt tgatcctgac ggcgtccctc tcaaaaccat tcttgagctt
     5881 gccaaagaac tcagcaagtc caccggaatc ataacaacgg acagcgtgac cggagccact
     5941 ccggccagtt tcgggtctca tgcaggcgat cgaggcgatg accctgccat atccacaggc
     6001 atcttcgata acagggttga cgtagtaatg gggggaggcc gcaaacattt tgtgccagga
     6061 accgacaacg gagcacgaat cgatggacgc aatctcctaa aagagctgca ggacgaagga
     6121 ttcacagtgg tcgacaatgt ggatgacctg aaagcagata acaaccttag ggcatgggct
     6181 ctgctcaatt cgggttcact cactggggcg gcatcgcgga actacactct aggggaccta
     6241 acagaggagg caatcaggcg cctttcccaa aatgaaaatg ggttcttcct catggtggaa
     6301 ggagaccaga tagatggggg cgggcataat aatgatggct acagagtagt cacagaaatg
     6361 cttgattttg aaaccgccgt tcaggtcgcc gtcgacttcg cttcgggaag agacgatacg
     6421 ctcgtggtag tcgtagccga ccacgaaact ggaggtctcg gcattacgag tggcagcgcg
     6481 tacggaacat ccgcagaaat aggttgggtg cacaccgggc atacaggcag tcccgttgca
     6541 gtatacagct tcgggccaaa ttccgtcctg ctgggcagcc atatgtatct ggctaacata
     6601 cccaagatta tctcgctcgg atggggagtc acagacttcg ggcctgagcg ggaactgaca
     6661 gggccaggag ctttttgacg catctgccgc acactgtagc gatagatgcc catgaataac
     6721 taatccacgg caaaagccaa tcgagtgcgc gaggggagtt cctctcttcg cgcactcgat
     6781 tgatgtactt ctataggtaa acaacctcaa agtgaaatca tgatgccttg aagtacgacc
     6841 tggcg
//
LOCUS       DAOSYL010000092         6639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_9807_length_6639_cov_7.314247, whole genome shotgun sequence.
ACCESSION   DAOSYL010000092 DAOSYL010000000
VERSION     DAOSYL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_6639_cov_7.314247"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(687..1865)
                     /locus_tag="PLU15_10220"
     CDS             complement(687..1865)
                     /locus_tag="PLU15_10220"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOO31322.1"
                     /translation="MPKLDFLAQELESLHEQNLFNVIRTIEGPQGGWIVVDGKRVLNL
                     CSNNYLGFADHPELKSAAKKGIDQYGVGPGAVRSIAGTQSIHLELERKLAEFKHAEAC
                     IVLQGGFVANLAVIPAITGDGDIIFSDELNHASIIDGCRLSKAKVVRYAHNDTADLKN
                     KIMEHKDSARRMLIITDGVFSMDGDIAPLPEIVEVADEFGAMVMADDAHGEGVLGDSG
                     RGIVNHFNLEGKVEIEVGTLSKAFGVMGGYITGPKVLIDYLKQKGRPFLFSSGLTAAD
                     TAAAIAAVDVLVESDELVRKLWDNAAYFQKGLKEAGFDIGKTQTPITPVMLGDEGTAV
                     TFSKKLFDKGIFAQSIAYPTVAKGKARIRCMLSAAHQKEDLDFALATFTEVAKEMGVL
                     "
     gene            2548..3552
                     /locus_tag="PLU15_10225"
     CDS             2548..3552
                     /locus_tag="PLU15_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOO31323.1"
                     /translation="MNRLYPLSFIPLFKEKIWGGRRFESWFPVIPDGPIGEAWVLSAH
                     PHGLTPTENGYLANKTIPELLTEYGEDVIGKAWADQDYSDPPQCCKFPLLIKLLNSND
                     DLSVQVHPGEEYAATHLEASAKSEMWLVLEADPGTSIVYGLKEGIGPREFAEAAKSGS
                     VMDYLNEVQVSAGDVIAIPPGTIHALGAGLVVAEVQQSSDTVYRIWDYNRPGLDGKPR
                     ELHIDQALEVIDYSRPPEITQPKMASPNSGVSIGSFAGFDVQLARCCGRWERRPSPSS
                     FTAFLFLGGTGEVAWGEDERAHISVRPGSSVLIPASCPDYALRSVSSDLVFLEVSRAL
                     "
     gene            complement(3536..4687)
                     /gene="mtnA"
                     /locus_tag="PLU15_10230"
     CDS             complement(3536..4687)
                     /gene="mtnA"
                     /locus_tag="PLU15_10230"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOO31324.1"
                     /translation="MMRKTDMDKGDDLLSTTSSGRKGSGRPVRLEPITWDGDKRVVRL
                     IDQTLLPTELRYIDISAMDAMCEAIAELRVRGAPAIGIAGAFGVYLGVANRSFSSEKE
                     FFEVLDGVCSEVACVRPTAVNLAWAVKRVKRVAGEVADSAHSRGERADPEALVEAILS
                     EAIIMIDEDNKVCRAIGEYGASLIPDGASILTHCNAGALATAGWGTALAALYVAREQG
                     KEFRVWADETRPLLQGSRLTAFELMEAGIDVTVICDNMAASLMGRGEIDLVILGADRV
                     AANGDVCNKIGTYGVACLAHMHDIPFFVACPTSTFDLSLASGAEIPIEQRNPREVTHG
                     FGKQTAPDGVKVYNPAFDVTPHNLMSAIITERGIVRAPYGPGLAAIVRD"
     gene            4843..6156
                     /locus_tag="PLU15_10235"
     CDS             4843..6156
                     /locus_tag="PLU15_10235"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOO31325.1"
                     /translation="MNSLSFPEDFLWGVATSGHQVEGNNTASDWWAWEPGKIEGGLTS
                     GRACDAWNRYEEDYATMKSMGLNAYRMGVEWARVEPRPGMWDDAAFAHYEAMMESLSE
                     KGIAICLTLYHWVLPKWVADQDGWENPATCDAFLRFVQEVVNRLGKYPLLWCTLNEPN
                     VYTMFSYVTGEFPPEKRSLRAARRVTRHLLTAHAGAYSIICEGGSKSADPNSRPLIGI
                     AHNMTDFAPASHSPFDRAIAKYQSKSWNSSYIEALVTGRMPGPFGDGKPIPGLAGSLT
                     YMGLNYYTRRTVAFRPNGISNAFSQEVFPRGTELNDFGWAVCPEGIYNCLVELGKLRI
                     PVYITENGVADANDSMRPRYIVEHLREVWRAIGSGVDVRGYFYWTFQDNFEWRAGYTQ
                     KFGLFELDPTDPNLQRTPRRSAEMYAKIAKANGITESISTKYLGK"
BASE COUNT         1570 a         1992 c         1612 g         1465 t
ORIGIN      
        1 gtccggccca tatggccagc tccccatata gccacggtat gcaccctaac gccctccttg
       61 acattgtaag cctgtgtcag gtcggacttc agaacgactg caaccttagc agtcgcaatg
      121 cccgccttca gcatcatctt tttgatctca tgcctatgat actgacagaa atatatgcag
      181 tagaataacc agagcctatg gccccgattc gttatattcc tggacataca gtaagacatc
      241 tcttgcctat caaaagccaa tcagtttctg caaaagtcag atgtactggc attcctgcca
      301 tttcaatgcg atatcaatat accatcggct cctaatagtg tcaattttct agactattca
      361 tcgcttgccc agctccatgc atgtttgact cgtgaggtat tacgcttttg tatgatcgtc
      421 actaatacca tgcaataatt caaagccatc tctttagaac atgattactc ttattcgagc
      481 tgtcagattg ccaacacctg acgcgaaacg cccaggctat cacagctggg cgctcagcgt
      541 atcgctcagg cacattttca gagatacttc tcacaaaaga tagatgtttg aatgtgaaag
      601 cgcctctcat cacgactcca atgtccacaa gctgccttgc agaaatgtca tgccgagagg
      661 cgcctatata tgccgcaggg tcgccgctag agaaccccca tctccttggc cacctcggtg
      721 aatgtggcga gggcgaaatc caagtcttcc ttctggtgcg ccgccgacag catgcacctt
      781 atcctcgcct tgccctttgc cacagtcggg taggctatcg actgggcaaa aatcccctta
      841 tcgaatagct ttttcgagaa cgtcactgca gttccttcgt cgccgagcat tacgggggtt
      901 atgggcgttt gggtcttgcc tatgtcaaac ccggcttcct tcaaaccctt ctggaagtat
      961 gcagcgttgt cccacaactt cctgacaagc tcatcgcttt cgacgagaac atcaacggcc
     1021 gcaattgctg cagcggtatc cgccgccgta aggccggaac tgaacaggaa cggacgcccc
     1081 ttctgcttca ggtagtcgat gagaaccttt ggcccggtta tgtatcctcc catgacgccg
     1141 aaagccttcg acagcgtgcc cacctcgatc tctaccttgc cttccagatt gaaatggttg
     1201 acgatacccc gaccgctgtc tccgaggacc ccttcgccgt gggcgtcgtc cgccatcacc
     1261 atggcgccga actcgtctgc aacctcaacg atctcgggaa gaggtgcgat gtccccgtcc
     1321 atgctgaaca ccccgtccgt gatgatcagc atcctgcggg cagaatcctt gtgctccatg
     1381 atcttgttct tcaggtccgc agtgtcattg tgggcatacc ggaccacctt cgcctttgac
     1441 agcctgcaac catcgataat gctggcatgg ttgagctcat ccgaaaagat gatatcgccg
     1501 tcacccgtaa tcgctggaat aacagcaagg ttcgccacga acccaccctg aaggactatg
     1561 caggcttccg cgtgcttgaa ttccgcgagt ttccgttcga gctcaagatg gatgctctga
     1621 gtgcccgcaa tcgacctgac cgccccgggg ccaacaccat actggtctat gcctttctta
     1681 gctgcgctct tgagctcggg atggtcggca aaccccaagt agttgttgga gcaaaggttc
     1741 aaaaccctct tgccgtccac tacaatccag ccgccctgcg gcccctcaat cgtccttatc
     1801 acattgaaca aattctgttc gtgcaaggac tcgagttcct gcgccagaaa atctagttta
     1861 ggcataagct aaccccccgt ttgcttgtct caatccgcgc tcccgtgcga gaagcggtgc
     1921 cctgaccgcg ttgaccaggg caacgataat gccattacca ttgcataatc cccgaaacta
     1981 taccacttat aactatatca ctagaggacc tcaaggcata ggttttccag cctccccaag
     2041 catgtcttgg tagctgcact tcgctaacac cttgcatgat caacacagat cacggatctc
     2101 aaggcccaca caggaaagcg gagcacactg gaaaagagaa atgacaagca tctgaatgca
     2161 gccatacata agtgaattcg ttattgagga ttaatctccc ttgcgacctg gccagttcga
     2221 gaagatcatc gtcgctgatt cttggacaac tttccgagat gccaagcacg tcatggccaa
     2281 aggcccccaa ttacaataca gtgcaaaatg ggaaattctc gttcgccaac gcttccattg
     2341 ttccctgcga gtcaaagcat agaattcccc attttccaga cacttagctg caaagactaa
     2401 tacgggctga gagtgcttta ggatcaactg cggacagttt tccacccacc ctgctttgac
     2461 gtctttaccc ccagctgcaa cccatccaat gttgaccgac ccaatcccta acagtaagat
     2521 gtagttagca ctgtgaggtg tttgagattg aacagactct atccgctatc attcattcct
     2581 ttattcaaag agaaaatctg gggaggacgt cgtttcgaat catggtttcc cgtaatccct
     2641 gatgggccca taggggaagc atgggtcctt tcagcccatc cacacgggct gacccctaca
     2701 gaaaacggat acctggcaaa caagaccatt cctgagctcc ttacggagta tggagaagac
     2761 gtaatcggca aggcttgggc cgaccaggac tattccgacc cgccgcagtg ctgcaagttt
     2821 cccctcctca tcaagctcct caactccaac gatgacctct ctgtccaggt gcatccgggt
     2881 gaagaatatg cagcaacaca cctggaagca tcggccaaat ccgaaatgtg gctggttctt
     2941 gaagcagatc caggaacctc aatagtgtat ggcctcaaag aaggcatcgg cccaagggaa
     3001 ttcgctgagg ccgcgaaaag cggatccgta atggattacc tcaacgaggt acaagtttca
     3061 gccggagacg tcattgccat accgcccggg accatccacg ccctaggcgc aggtcttgtg
     3121 gtcgcagaag tccaacaaag ctccgacacg gtctaccgca tctgggacta caacaggcca
     3181 ggccttgacg gaaagcctcg cgagctccac atcgaccaag cgctagaagt gatcgactat
     3241 tcacgcccgc cggagattac gcagccgaaa atggcgtcgc caaactcggg cgtatccata
     3301 ggctcgttcg caggcttcga cgtccaactc gcaaggtgct gcggcagatg ggaacggcgc
     3361 ccatcgccct cttcgttcac tgccttcctg ttcctcgggg gtacaggaga ggtggcatgg
     3421 ggagaagatg aaagggcgca catttccgta cgccccggat catcagtatt gatacctgcg
     3481 agctgccctg attatgcact ccggtcggta tcgtccgatc tcgtattctt ggaagtcagt
     3541 cgcgcactat agcagcaagc cccggcccgt atggcgcgcg aactatcccc cgctctgtga
     3601 tgatcgccga catgagattg tgcggtgtca catcgaaagc cgggttataa accttcacac
     3661 catcaggagc agtctgcttt ccgaacccgt gggtcacttc ccgcgggttc ctttgttcta
     3721 tgggaatctc cgctccgctg gcaagcgaca agtcaaaggt ggacgtgggg catgccacga
     3781 agaacggaat gtcgtgcatg tgcgcaagac aagcaactcc atatgtcccg atcttgttgc
     3841 atacatcccc attggcggca acacggtcgg ccccgagaat gaccaagtcg atctcgcccc
     3901 tgcccatcaa agaggcagcc atgttgtcgc atatgaccgt tacatctatc cccgcctcca
     3961 tcagctcaaa agccgtaaga cgcgatccct gcaaaagcgg cctggtctca tccgcccaga
     4021 ctctaaactc cttgccttgc tcccgggcca cataaagagc cgctaaggct gtcccccagc
     4081 ctgcagtggc cagggccccg gcgttgcagt gggtcagaat agatgcgccg tcaggtatga
     4141 gactcgcacc gtactcccct atcgccctgc ataccttgtt gtcctcgtct atcatgatta
     4201 tggcctcgct caggatagcc tccacaagtg cctccgggtc cgccctctct ccgcgactat
     4261 gggcgctatc cgcaacctcc ccggccacgc gcttcacccg cttgacagcc catgccaagt
     4321 tcactgccgt cggccgcacg cacgccacct cggagcagac accatcgagg acctcaaaaa
     4381 actctttttc agacgaaaag ctcctgttag caacccccaa gtagacaccg aacgcgcccg
     4441 ctatcccaat cgctggcgcc ccgcgcactc tgagctccgc tatagcctcg cacatcgcgt
     4501 ccatagctga tatgtcgatg tatcttagtt cagttggaag gagagtctgg tcgatgaggc
     4561 ggaccactcg cttgtcgcca tcccatgtga tcggctccaa acggacaggc ctaccgctgc
     4621 ccttcctccc cgaagatgtc gttgacaaca gatcatcccc cttgtccatg tccgtctttc
     4681 tcatcatctt tctcggacat cctcggtctt tcctgccaac ccgccgccat atccgcattt
     4741 cataaggtcg ccggatgcgc cctatgccat ggcggtttgc agtcgcacgc atggaggagt
     4801 atggacgcat caggcgaatc tgcacaccag gaggtggcat cagtgaatag cctttccttt
     4861 cctgaagatt tcttgtgggg cgtagcaaca tccggacatc aagtagaggg caacaacacc
     4921 gcaagcgact ggtgggcatg ggaacccggg aagatcgaag gcggcctcac atcaggccgg
     4981 gcctgcgacg cgtggaacag atacgaggaa gactacgcga ccatgaagtc catggggctc
     5041 aacgcctaca ggatgggagt tgaatgggcc cgagtggagc cgcgccccgg catgtgggat
     5101 gatgccgcct ttgcccacta tgaagcaatg atggaatctc tgtccgaaaa gggaatcgca
     5161 atttgcctca ctctttacca ctgggtgctg ccaaaatggg tggctgacca ggacgggtgg
     5221 gaaaatcccg caacatgcga tgcattcctc cgcttcgtgc aagaggtcgt aaaccggctc
     5281 ggcaagtacc ccctcctttg gtgcactttg aatgaaccca acgtctacac tatgttctct
     5341 tacgtgaccg gcgagtttcc gcccgaaaag cgatccctca gagccgcccg ccgagtcacc
     5401 cgtcacctct tgaccgccca cgcaggcgcc tactcaatca tctgtgaggg cggtagcaaa
     5461 agcgccgacc cgaacagcag gccacttatt ggcatagccc acaacatgac ggactttgcc
     5521 cctgccagcc acagcccgtt tgatcgcgcc atagcgaaat accagtccaa gtcctggaac
     5581 agctcataca tagaagccct agtcacagga cgaatgccgg gcccattcgg cgacggaaaa
     5641 cctatcccgg gtttggctgg atccctcaca tacatgggac tcaactatta caccagacgg
     5701 accgtagctt tccgcccaaa cggcatctcc aacgccttca gccaagaagt cttcccgaga
     5761 ggcacagagc tcaacgattt cggctgggcg gtttgcccag aggggatata caattgcctt
     5821 gtggaactcg ggaaacttcg tatcccagta tacatcactg aaaatggagt ggccgatgcc
     5881 aacgactcga tgcgcccgcg ctacatcgtt gaacacctcc gggaggtgtg gcgcgcaatt
     5941 ggcagcggcg ttgacgtgcg cggctatttt tattggacct tccaagacaa cttcgaatgg
     6001 cgtgcaggat acacacagaa gtttggcctc ttcgaacttg acccgaccga tccgaacctg
     6061 caaaggacgc cccgaagaag tgcggagatg tatgcgaaga tagccaaagc caacggaatt
     6121 acggagagca tttcaacaaa atacctgggg aagtgagcaa tgccacggaa aagcaaagct
     6181 tcgcgcaaaa agcgagggga ttacaagagc ctgtctaagt cactccccat aagtttttgc
     6241 gctatcggtt agcctgccat atgatgtctg ctaggcgtgg acacgtcttt gcacctatgt
     6301 cgcagtgcga taattggttc gcatttgtac atcgacaacg tacacatctt ggccttacca
     6361 taaatgcccg cagcactcag ccgcgccggg atatgatcct cggggcaaag ccagggtaga
     6421 tcgaaatccc ggttcgacgc tcgcgggcaa ccatcttctt tacaaccacg aaagcgacgc
     6481 gcatacgctc ccgaccatga gatccatatt agcgctccag cagtttatgc attcatatga
     6541 aaaggcgact tgggattgcc ttcaaggaat gtgtctatta tttagtcgct tttcgggcgc
     6601 gggtgtcaaa gttttagact ggtctgtaaa acctgctcg
//
LOCUS       DAOSYL010000093         6630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_9826_length_6630_cov_7.434221, whole genome shotgun sequence.
ACCESSION   DAOSYL010000093 DAOSYL010000000
VERSION     DAOSYL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9826_length_6630_cov_7.434221"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLU15_10240"
     CDS             <1..191
                     /locus_tag="PLU15_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31326.1"
                     /translation="ADPASEQELAKLERENPGAIIVGSIIAAQAFPGRVLAMVPAAGF
                     ERVPPDQKRMRDDKFTRF"
     gene            243..425
                     /locus_tag="PLU15_10245"
     CDS             243..425
                     /locus_tag="PLU15_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31327.1"
                     /translation="MAENKRVRLTIWVDFEHREWLRRQAYERDTTIAQVVHDLIEDAK
                     LQEVKGTGKGGKDVEL"
     gene            581..1102
                     /locus_tag="PLU15_10250"
     CDS             581..1102
                     /locus_tag="PLU15_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31328.1"
                     /translation="MTALKSELLDKGYRVALLEIDGPNPNAMPITELVIPEGATAQPV
                     QDIDDDPLFEDVLLLEIPDVPEREKPMIMTLTQYRRWCRALYTEPELYCRAMLERLGI
                     TFATLDRLGKAVMRVYSTDDGPLVLDAMTGNLNHDALAREDIIIGDSEPQASLETLGR
                     VVSELGPELTNPS"
     gene            1116..1655
                     /locus_tag="PLU15_10255"
     CDS             1116..1655
                     /locus_tag="PLU15_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31329.1"
                     /translation="MSKELTPMTTKIRVRFQQYGTWGGRGFTGILTWVEAPPIDILGL
                     VEQSETPAQVPVIILDGEQDGWSGLQLFPGQTLPGRFASGYCNSLAGKYYIGRLEIGA
                     NADRLKSAGPPSDVFPGKPSYLHLVEPDTPLTAEQRELLLEAIRYDREPRFAAGADIT
                     ALFSREELEGEKRGQIFYA"
     gene            1689..2012
                     /locus_tag="PLU15_10260"
     CDS             1689..2012
                     /locus_tag="PLU15_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31330.1"
                     /translation="MDKTQVFTVNLTPEQHDWLRWQSFETREPMTAIVRRLIDNARTN
                     GVRHDYGTTSGPTTQGFEAILTLEQYEWLRREAFETRQPMTVIVRRLIDDARRGGEKD
                     GEGRP"
     gene            1993..2658
                     /locus_tag="PLU15_10265"
     CDS             1993..2658
                     /locus_tag="PLU15_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31331.1"
                     /translation="MVKVDRSNGMLAVTCPYSKSFVSFAKMRGGKWSDGTKQWMFDER
                     DEFAIRSTLIDIYGTDDYESCEKTDVRIELDELQSLVKGRDRLFFLGRELVRRRYYDN
                     YVTLGTGVVMLEGGFPREAGGYREPELKPHKGTVLEVRDVPVTAAEAALKKWPEAVTV
                     LGEYDLEALQAERDFHAKRIKELDALIARYSGVFQEDEIIADLQDDDDEPPTSGSSET
                     VEG"
     gene            2591..4033
                     /locus_tag="PLU15_10270"
     CDS             2591..4033
                     /locus_tag="PLU15_10270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO31332.1"
                     /translation="MLTCKTTMMSHQQAAVQKLSRVKVGALYMDMGTGKTRTALELAI
                     KRLNAGKVDCILWCCPVSVKQTIADETDKHVNGATWEMAQPQRIEDPNADIYIAGIES
                     LSSSLSLNVRLMELVQAKRCFLVCDESSLIKNHTANRTLALWRLGERCEYKLILNGTP
                     LSNNEQDLFSQWYFLDPRILGYNSYYSFAANHLEFDPDYPGRVVAAHDVEYLTRKIAP
                     YTYQVRKDECLDLPPKTYSWRYCYMTEEQRHIYEEAKASILMDIDLDDWTSHTLFRLF
                     TALQKVVSGIRPDRVPIFEKPMNNPRIEALVETVQDLPQDAKCIIWCKYTHEINTLQQ
                     VLEPYGGVAVIHGGVAPAKRDQEIKRFREDARFLVANKRVGAFGLNLQHCNYAIYYGN
                     DFSWETRTQSEDRIHRAGQMHNCHIIDIVCADSIDMRIQHCLTNKEQLVDSFRKEIDR
                     TKDKTSLGRWIDGGEDLPTEERIPGGQGTA"
     gene            3978..5273
                     /locus_tag="PLU15_10275"
     CDS             3978..5273
                     /locus_tag="PLU15_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HOO31333.1"
                     /translation="MAKTYLQKNVYQAAKERLELIFDEFEHIYFSFSGGKDSGVMMEL
                     ALEVARERNRLPLHALLIDLEGHYREHIEYSLRMCDRPEIKPYWICLPLSLRNAVSQF
                     EPKWVCWDEDNRDIWVRQMPDRPYVITEHNMPQEWSWFHKGQEFEEFILYFADWFADY
                     YHADKTACVVGIRADESMNRYRTIVNDRKTRYADQGWTTLVKGLRNTVYNAYPIYDWR
                     VEDIWTYVGKSGCDYNKIYDYMYKTGRSIHDMRICQPYGDDQRKGLDMFQECEPETWF
                     KVVQRVSGANFGAIYRGGTILGNIKVELPPGHTWESYAKLLLNSMPPYLKEHYIEYFN
                     TFFRWWDRFGGGRTEHQTVVTDEDKYDEMGIYYSDKIPYDGYQGDDVPDSPKWRQPLW
                     KRICKVLLKNDYWCKELSFSQVKGEYERLESLKEKYRDI"
     gene            5286..5891
                     /locus_tag="PLU15_10280"
     CDS             5286..5891
                     /locus_tag="PLU15_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31334.1"
                     /translation="MEIHDIGGHRLTLAELGQDDCAGVLDRRAHVVYNAPPNPDTINW
                     WRGYKQVSYTYQDFLDALFALYDQADADEYYIETGKVHDSVVDHLRGRGLYIQERPIL
                     YSAPLNGHGLGMAKRRIPCTLVVASRQPVPLPAGEYSHETIADALSRRQPGETVFDPC
                     IGKGLALRAAIARGMRCVGIESSPERLKCCIEYATREAERC"
     gene            5930..6520
                     /locus_tag="PLU15_10285"
     CDS             5930..6520
                     /locus_tag="PLU15_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044260 - cellular macromolecule metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOO31335.1"
                     /translation="MRDTLGAMDTEERIEAINELRGVLHELSPFRAEPVDYVQWVPAE
                     QVTANDYNPNAVAPPEMRLLYISIREDHFTQPIVSARDAENNHYIIVDGFHRNRVGKE
                     YRDIRERLHGYLPIVTTDKPLAERMASTIRHNRARGKHAVSPMSDLVAALYRDGWPDD
                     RIAKELGMELDEVLRLKQITGLPEIFANREYSKAWE"
BASE COUNT         1658 a         1657 c         1948 g         1367 t
ORIGIN      
        1 aggctgaccc ggcgtccgaa caggaactgg cgaagctgga gcgggaaaac cccggagcaa
       61 tcatcgtcgg cagcatcatc gctgcccagg cattcccagg ccgggtgcta gctatggtgc
      121 ctgcagcagg cttcgagagg gtcccacccg accaaaagcg gatgcgggac gataagttta
      181 ctaggttcta gttgtatggc tcgccgggag ccgctaatcc tggcagaaag gaggccccgc
      241 caatggcaga aaacaaaaga gttcgtctta ccatatgggt cgattttgag catcgtgagt
      301 ggctgcgacg gcaggcctac gagcgtgaca ctacaattgc gcaagtggtg cacgatctca
      361 tagaggatgc aaagttacag gaggtcaagg gcaccggcaa aggaggtaaa gatgttgagc
      421 tatgacaaag tcggttttga acgaattgta gttggcgaac ctcaaggctc acgtcttcgc
      481 ctgctcaaag cgagccgcga tcaaggacga ctggaacatc ttttgggatg agaccagcct
      541 agccattctt gggcaggctt aagaaatggg ggagagagca atgacagctc tgaagtcgga
      601 actgctcgac aagggttaca gagtagccct gctcgaaatc gacggcccca atcctaacgc
      661 catgcccatc acggagttgg tgatacctga aggggcgacg gcgcagccgg tgcaagacat
      721 cgacgatgat ccattattcg aggatgtgct cttgctagag atcccagacg tcccagagcg
      781 cgagaaacca atgattatga ccctgactca gtatcgtcgg tggtgccggg cgctgtatac
      841 ggaacctgag ctttattgcc gagccatgct ggagcgtctt gggatcacgt ttgccacgct
      901 cgatcggctc ggtaaggccg ttatgcgggt ctacagtacc gacgatgggc cgctcgtgct
      961 agatgccatg accggcaatc tcaatcacga tgccctggca cgggaggaca tcataatcgg
     1021 tgactcggag ccccaggcat cgctggagac gctagggcgt gtggtatcgg agcttggtcc
     1081 tgagttgacc aatccatcat aaggagggta atgacatgtc taaagagcta accccaatga
     1141 caactaaaat tcgagttcgg ttccaacaat acggcacttg gggtggtcgt gggttcacag
     1201 gcatccttac atgggtagag gcaccgccga tcgacatcct gggactggtt gagcagtctg
     1261 agacacctgc ccaggttccg gttatcattc tggacggtga acaagacgga tggtctggtc
     1321 tgcagctgtt cccgggccag acgctacctg gcagattcgc ctcaggttac tgcaattctc
     1381 tagccggcaa gtattacatc ggtaggttag agataggtgc taacgccgat aggctcaaat
     1441 cagcagggcc cccatccgat gttttcccgg gcaaacccag ctatctgcac ctagtggaac
     1501 cagacacgcc cctgacagca gaacagcgtg agttactgct tgaggcaatc cggtatgatc
     1561 gcgagccccg gttcgcagcc ggcgccgaca tcactgctct attctcacgt gaggagctag
     1621 agggtgagaa gcgtggacag atcttttatg cgtgatcttc atcaatccag cgacaggagg
     1681 taacgtaaat ggataagacg caagtattca cagtaaacct tacgccagaa cagcatgact
     1741 ggctacgctg gcagtccttc gaaacacgcg aaccaatgac cgcgatcgtg aggcggctca
     1801 ttgataacgc ccggacaaac ggcgtgaggc atgactatgg aacaactagc ggccccacga
     1861 cacaagggtt tgaagccatc ctcacactgg agcagtatga gtggctgcgc cgggaagcgt
     1921 tcgaaaccag acagccgatg actgttattg tgcggcgact gattgatgat gctcgaaggg
     1981 gcggtgaaaa ggatggtgaa ggtcgaccgt agcaatggga tgctggcagt gacttgcccg
     2041 tacagcaagt ccttcgtaag cttcgcaaag atgcgtggcg gcaagtggtc tgacggcacc
     2101 aaacagtgga tgttcgacga gcgggacgag ttcgcgatca ggtctaccct gatagatatc
     2161 tacggtacag acgattatga gagctgcgag aaaactgatg tgcgcattga gctggacgag
     2221 ctgcaatcac tggtgaaggg cagagacagg ctgttcttcc tcggacggga actcgtgagg
     2281 cgccgttact acgacaacta tgttactctg ggcaccggcg tagtaatgct cgaaggaggc
     2341 ttcccccggg aggccggagg ataccgtgag cctgaactca agccccataa ggggacggtc
     2401 ctcgaagtgc gtgatgtgcc ggtgaccgca gcagaggcgg ccttgaagaa atggcctgaa
     2461 gctgtaaccg tgctgggaga gtatgacctt gaagccctac aggcagagcg agatttccat
     2521 gctaagcgta tcaaggagct tgacgcactc atcgctcgct atagcggagt gttccaggag
     2581 gatgagatca ttgctgacct gcaagacgac gatgatgagc caccaacaag cggcagttca
     2641 gaaactgtcg agggttaagg taggcgccct ctacatggac atgggcactg gcaagacgcg
     2701 cactgcactg gagctcgcca tcaagcgcct caacgccggc aaggtcgact gcatcctctg
     2761 gtgttgcccg gtgagtgtca agcagacgat cgcggatgag accgacaagc atgttaacgg
     2821 ggcgacctgg gaaatggccc aacctcagag gatagaggat cccaatgccg acatctacat
     2881 cgccggtatt gaaagtctat catcgtcgct ttcgcttaac gtccggctga tggagctggt
     2941 tcaggccaag aggtgttttc tggtctgtga tgagtcatcg ctcatcaaga atcacacggc
     3001 caatcgcaca ctggctctat ggcgactcgg ggagaggtgt gaatacaagc tgatcctcaa
     3061 tggaacaccc ctctccaaca atgagcaaga tctgttcagc cagtggtatt ttctcgaccc
     3121 gcggatattg ggctacaaca gctattactc gttcgctgct aaccacctgg agttcgaccc
     3181 ggactaccca ggcagagtag tagcggcgca cgatgtcgag tatttgacgc ggaagattgc
     3241 gccctacacc tatcaagtgc gcaaggatga gtgcttggat ctgccgccga aaacgtatag
     3301 ctggcgatac tgctacatga cagaggaaca acggcatatc tacgaagaag ctaaagccag
     3361 tatcttgatg gacattgacc ttgacgactg gacgtcacac acgctgttcc gccttttcac
     3421 agctttgcag aaggtggtgt cgggtattcg acctgatcga gtcccgatat ttgagaaacc
     3481 gatgaacaat ccacgtatag aagcactcgt cgaaacggtg caggaccttc cgcaagatgc
     3541 taagtgcatc atctggtgca aatacacgca tgagatcaat acgttgcagc aggtactgga
     3601 gccctacggt ggagtagctg tgattcatgg aggtgtggcg cccgctaaac gagaccagga
     3661 gattaagcgt ttccgcgagg acgcacggtt tctggtggct aataagaggg taggagcatt
     3721 cgggttgaat ttacagcact gcaactacgc catctactac ggcaacgatt tcagttggga
     3781 gactcgcacg caatcagagg accggattca ccgtgcaggc cagatgcaca attgccatat
     3841 catagacatt gtttgcgctg acagcattga tatgcgcatt cagcactgcc tgacaaacaa
     3901 ggagcagtta gtggactcat tccgcaagga gatagatcgc accaaagaca agactagttt
     3961 ggggaggtgg atagacggtg gcgaagacct acctacagaa gaacgtatac caggcggcca
     4021 aggaacggct tgagctgata tttgatgagt tcgagcatat atacttctct ttctctggcg
     4081 gtaaagactc aggggtgatg atggagctag cactggaggt ggcgcgcgaa cgcaatcgtc
     4141 tgccactcca tgcgctccta attgaccttg aaggtcacta tcgggagcac atcgagtatt
     4201 cgctgcgcat gtgcgaccgg cccgagatca agccatactg gatctgtctg cccctctcgc
     4261 tgcgcaatgc agtgtcgcag ttcgagccca agtgggtctg ttgggatgag gacaaccgtg
     4321 acatctgggt tcggcaaatg cctgacaggc cgtatgtgat taccgagcac aatatgcccc
     4381 aggagtggtc atggttccac aaggggcagg agttcgagga gttcattctc tacttcgccg
     4441 attggtttgc tgattattat catgccgata aaactgcctg cgtagtaggg attcgagcag
     4501 acgaaagcat gaacagatat cgtaccatcg taaacgaccg caagacccgg tacgcagacc
     4561 aaggatggac tacgttggtg aagggtctgc ggaacacggt ttacaatgcc taccccattt
     4621 atgactggcg agtagaggac atatggacgt atgtcggcaa gagcggctgc gactacaaca
     4681 agatctacga ctacatgtac aagaccggca ggtcaattca cgacatgcga atatgccagc
     4741 cctatggcga tgaccagcgc aaggggttgg acatgttcca ggagtgcgag ccagaaacat
     4801 ggttcaaggt ggtgcagaga gtctccggag ccaactttgg cgcgatctac cggggcggga
     4861 cgattctcgg caatatcaaa gtcgagctac ctccggggca tacctgggag agctatgcca
     4921 agctgctcct gaacagtatg cctccgtatc tcaaagagca ctacattgag tattttaata
     4981 ctttcttcag atggtgggat cggtttggag gcgggcgcac cgagcatcaa acggttgtca
     5041 ctgacgagga caagtatgac gaaatgggta tctactactc agacaagatt ccatatgacg
     5101 gataccaagg cgatgatgtg cctgatagcc ccaagtggcg gcagccgtta tggaagcgga
     5161 tctgcaaggt gctcctgaag aacgattact ggtgtaagga actatcgttc agccaagtga
     5221 agggagaata tgagcgacta gagtctctga aagagaagta tcgtgacata tagggaggca
     5281 ctgctatgga gatccacgat atcggtggcc atcgcttaac gcttgcggaa ctgggccagg
     5341 atgattgtgc tggagttctg gatcggcgcg cccacgtcgt ttacaatgct cctcctaacc
     5401 ctgatacgat caactggtgg cgcggatata aacaggtttc atacacatat caggactttc
     5461 tcgatgcatt gtttgcgctc tacgatcagg cggacgccga tgagtactac atagagacag
     5521 gcaaggtgca tgattccgta gtcgaccacc tgcgcggccg agggttgtac atacaggagc
     5581 gccccatcct ctactcggcg cccctgaacg ggcatgggct gggcatggcc aagaggcgaa
     5641 taccctgtac gttagtggta gcatcgaggc aacctgtgcc gctaccggcc ggtgaatact
     5701 cgcatgagac gattgccgat gctctatcgc gccggcagcc aggcgaaacg gtgttcgacc
     5761 cgtgcatcgg caaaggcttg gctcttcggg cagccatcgc gcggggtatg cggtgtgtcg
     5821 ggatagagtc gagcccagag cggttgaagt gctgtatcga atatgcgaca agggaggcag
     5881 agcggtgctg agtctatttg catcaacatt ggatgatatc aaggcctcaa tgcgcgacac
     5941 actgggcgcc atggacacag aggagcgcat agaggccatc aacgagcttc gcggggtgtt
     6001 gcatgagcta tcgccgttcc gggcagagcc tgtggattat gtccagtggg ttccggcaga
     6061 gcaggtgacg gccaacgact acaatcctaa tgctgtcgcc ccgccggaga tgcggttgct
     6121 ctatatcagc atccgtgagg accacttcac acaacctatc gtgtccgctc gcgatgcaga
     6181 gaacaatcac tacatcattg tcgacggttt ccatcgaaac cgcgtgggca aggagtaccg
     6241 tgacatcagg gaacgtctgc atggttacct gcctatagtt acgacagaca agcccctcgc
     6301 cgagaggatg gctagcacca tacggcacaa tcgggccagg ggcaaacatg ctgtgtcgcc
     6361 catgtcagat ctggtagcag ctctataccg cgatgggtgg ccagatgaca gaatcgctaa
     6421 ggaactgggc atggaacttg atgaagtatt gcggctcaaa cagatcacgg gactgcctga
     6481 gatattcgcc aatcgcgagt actccaaggc gtgggaatag agtgaaggaa gagaggctaa
     6541 tagaatgaac tttgaacgaa ttgcagtcgg agagccattg gctggtgctc gggaagctca
     6601 gctacgcaac caaactctga tgctccttga
//
LOCUS       DAOSYL010000094         5868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_11438_length_5868_cov_7.450198, whole genome shotgun sequence.
ACCESSION   DAOSYL010000094 DAOSYL010000000
VERSION     DAOSYL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11438_length_5868_cov_7.450198"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..2316)
                     /locus_tag="PLU15_10290"
     CDS             complement(139..2316)
                     /locus_tag="PLU15_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOO31336.1"
                     /translation="MHSNERGSLQVVGIVILLLILVGIGVFSGILAGYLKNAPSVEDV
                     DIESHRSLPTVIYDAGGQVLTRLMVENRAWVPLSSVPSDLVNAIVAVEDHTFYDHHGI
                     SLRRIAGALWHDLKHMSPDQGGSTITAQLARNVFLSQEKTIDRKIWEALYAFQLERRY
                     TKDEILEYYLNWIYMGHGVYGVEAASQLYFGKPVTELSLGQCALIAGLAKGGEVYSPY
                     KNMEAALQRRQIVLKRMADVGYISPEEASIAADEPVKLVGLKESGNTVGKYVRYMVRD
                     YLTGKYGKDAVYRGGLTVKTTIDLKAQQAAEQAFTSLLPTGRIEEREGYSLTYPQCAL
                     VAIDPQTGAIRALVGGRGEDEYNRATKAMRSPGSSMKPFVYTAAIDSGYTPATTMVDE
                     PISYPMGDGSMWSPKNYNGKYNGVMTLRQALEQSTNTIAVKLAKELGPETVIHYAKKM
                     GITSLVERGAKNDKGLALALGGLTQGITPIEMAQAYSVLANGGVKVTPYLVTEVRDAN
                     GVVLESHGPNREIVLDERTAYIMADMMKGVITSPNGTGKRADIGRPAAGKTGTADSNT
                     DAWFVGFTPDLAAAVWIGEDTLREMRYPNYGVVGSGKAAEIWGSFMKQALAGTAPRDF
                     TVPEGIISGVRVCKVSGDLATSNCPSGSVIYEKFIAGTEPTRSCRVHGGYAKPKTEPE
                     SEPRLENDPFDQVQAVPQTPPYTDSEASPQIEYSDSQTPPWEQ"
     gene            2490..3710
                     /gene="tyrS"
                     /locus_tag="PLU15_10295"
     CDS             2490..3710
                     /gene="tyrS"
                     /locus_tag="PLU15_10295"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOO31337.1"
                     /translation="MNPKEQLEIIERGAAEVISVEDLLAKFERSAKTGKPLKVKLGLD
                     PSAPDIHIGHTVVLYKMRQFQDMGHEGIIVIGDFTGRIGDPTGRSEMRKQLTEEQVRA
                     NARTYEEQIFRILDPARTRVTFNSEWLSKLDFADVIELASKVTVARMLERDDFENRYK
                     EGRPIGIHEFFYPLMQAYDSIALEADVELGGTDQKFNNLMGRTLQREYGQDPQCVVLT
                     PLLPGLDGVNKMSKSLGNYIGIDEEPFEIYGKTMSIPDELIVAYVDSATTMSVAEKKG
                     IEEGLASGTLHPMTAKRRLAFQLVLQFHGEDAARGAQDEFDRVFKHGEHPDEMPEVRV
                     SQDDLVDGKIAVSKLVVIAGLASSNSEARRLIEQGGVRIDDVRVEGVMDEVEPVNGMV
                     INVGKRRYAKVIRE"
     gene            3729..4652
                     /gene="fba"
                     /locus_tag="PLU15_10300"
     CDS             3729..4652
                     /gene="fba"
                     /locus_tag="PLU15_10300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOO31338.1"
                     /translation="MSLVASKEILDAANEGRYAVGAFNVNNLEFAKGVVSAAEEERSP
                     AIIQVSESAIAYAGIEEIACIVRSLAKDVGVPIALHLDHGRKFDVIMQCIRHGFTSVM
                     IDGSNLPLDENIAITKKVVEAAHGAGVSVEAELGRLAGIEDDINVQARDAFLTDPEEA
                     AIFVRETGVDALAIAIGTSHGPRKFKGEPVLALDLVTEIKEKVGIPLVLHGASGVSTD
                     VIEQAEKYGAKWGGSKGVPDESIVEAINRGINKVNIDTDMRLAFVASIRETLSTRPEV
                     TDPREVLRPAMAAIKSVAAGKMRLFGSSGQA"
     gene            4994..5659
                     /gene="yunB"
                     /locus_tag="PLU15_10305"
     CDS             4994..5659
                     /gene="yunB"
                     /locus_tag="PLU15_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HOO31339.1"
                     /translation="MNRRMRSVVLVALFCVCMGFLGFVIVEVRLKPTLFELAEARARA
                     VATTAVNSALSETGALDIKYEDLMDWKTDGLGNIVAVQPNTGEINRIAASTTTRVQEA
                     LQEIERVRISVPIGQVFGSALLAHVGPWLTITVVPIGVVSTTVTDKFETAGINQLRHR
                     IYLEIEAYVKILVPLVTSNVRVMTSMPIAEAIILGEVPNVYVHVGEPDKVLRGILGGP
                     GDE"
BASE COUNT         1342 a         1410 c         1716 g         1400 t
ORIGIN      
        1 ccggagctta gcgattggcg agcgaggcgt cgatggttgg cagcaaacag agcccggggt
       61 cttgtcagac ctcgggcttt cctgtatgtg tgtttcatgt tcttgttaac gatctgtttc
      121 ggttgcggtt tggaaggttc attgctccca tggcggcgtc tgcgaatctg agtattcgat
      181 ctgcggagag gcttcgctgt cggtgtaggg tggagtttgg ggcacagcct gcacttgatc
      241 gaaaggatca ttttcgagac gaggttcgct ttcaggctcg gtcttgggct tcgcatatcc
      301 cccatgcact ctgcaagaac gggtcggttc tgtgcctgct atgaatttct cgtagatgac
      361 cgatcctgat gggcaatttg atgttgccaa atcaccagaa accttgcaca cgcggacacc
      421 tgagattatt ccttcaggta cggtgaaatc gcgcggagcg gttccagcca gagcttgctt
      481 catgaaggag ccccaaatct ccgcggcctt cccgcttccg acgacgccat agttgggata
      541 ccgcatctcc ctgagagtgt cctcgcctat ccagacagct gcagccaggt caggtgtgaa
      601 tccgacgaac catgcgtccg tattgctgtc tgccgtaccg gtctttcctg cggcaggtct
      661 gccaatgtcg gcgcgctttc cagtgccgtt cggggaggtt atgacgcctt tcatcatgtc
      721 cgccatgata taggcagtcc tttcatctaa tacgatctcc ctgttggggc cgtgtgactc
      781 gaggactact ccgttggcat cccgaacttc ggtgacgagg tagggcgtga ccttgactcc
      841 tccgttcgcc aacacgctgt acgcctgcgc catttcaata ggggtgatgc cttgggttag
      901 tccgcctaag gccagggcca accctttatc attcttggcg cctctttcca caagagatgt
      961 gattcccatc ttctttgcgt agtggatcac agtctctggt cccaattctt tggccagctt
     1021 gacagcgatg gtgtttgtcg actgctcgag cgcttgcctc agcgtcatga cgccgttgta
     1081 tttcccgttg tagttttttg gagaccacat cgagccatct cccatggggt atgagattgg
     1141 ctcatcaacc atggttgttg caggagtgta acctgagtcg atcgcagccg tgtaaacgaa
     1201 tggcttcatg gacgaaccgg gagaacgcat ggctttggtg gcccggttat actcgtcttc
     1261 acctcgccct ccgacgaggg cccttatcgc cccggtctgg ggatcgatag caacaagggc
     1321 acactgaggg tatgtcagcg aatacccttc gcgttcctct attctgcctg tcggcagcaa
     1381 actcgtgaat gcctgctctg ccgcctgttg ggccttgagg tcgatcgtgg tctttactgt
     1441 taggccgccg cggtaaactg cgtcctttcc atatttgcct gtcaagtaat cgcgaaccat
     1501 gtaccttacg tattttccta ctgtgttgcc ggactctttc aatccgacga gcttgactgg
     1561 ctcgtctgcc gcaatcgaag cttcctctgg gcttatataa cccacgtcgg ccatgcgctt
     1621 gagcactatc tgccgtcttt gcagcgctgc ttccatgttc ttgtaaggag agtagacttc
     1681 gccgcctttg gcgagtccgg cgataagggc gcattggccg agagacagct cagtcacagg
     1741 cttgccaaag taaagctgtg aggcagcctc aaccccatag acgccgtggc ccatataaat
     1801 ccaattgagg tagtattcaa ggatctcgtc ctttgtgtat cttcgctcga gctgaaaagc
     1861 atacaaggcc tcccagatct tgcggtcaat ggtcttctct tgcgagagga aaacattcct
     1921 ggctagctgg gcagttatgg tgcttccgcc ttgatcgggc gacatgtgct tgagatcgtg
     1981 ccacagcgcc ccggcaattc tgcggaggct tatgccgtgg tgatcgtaga aagtatggtc
     2041 ctcaacagcg actatcgcat ttaccaaatc agagggaacg ctgctcaacg ggacccatgc
     2101 tctgttttca accatgagtc gtgtaaggac ttgaccaccc gcgtcgtata tgactgtagg
     2161 aaggctgcga tgagattcta tgtcaacatc ctccacgcta ggagcgttct tgaggtaccc
     2221 tgccaagatc cccgagaaca ccccgatgcc tacgaggatg agaaggagaa tgacaatgcc
     2281 gacaacttgg agagaacctc tctcattcga atgcatgatg attgaaaggc ctcctcgcct
     2341 caataattct gctacattat aacatagtga tttacatgcg caaacactgg accggagggg
     2401 cgactggaag cggctggtct tcattgaccc aggttgctgc cctgtgctag actcaatgta
     2461 tagttgtaga tttcgggggg cattgggcta tgaatcccaa ggaacagctg gagattattg
     2521 aaagaggcgc tgcagaggtc atctccgttg aggaccttct tgccaagttt gagaggtcgg
     2581 caaagacagg aaagcctctc aaggtgaagc tggggcttga tccgtctgct cctgacattc
     2641 atataggcca cacggtggtt ctgtacaaga tgaggcagtt tcaagacatg ggccatgagg
     2701 ggatcatcgt aataggggat ttcaccggta ggattggcga tcccaccggg aggtcggaga
     2761 tgcgcaagca actgactgag gaacaagtgc gcgcgaatgc caggacgtat gaggagcaga
     2821 ttttcaggat tttggatccg gctcgcacgc gtgtgacgtt caactcggaa tggctttcga
     2881 aactagattt cgctgatgtg atcgaacttg cgtccaaagt cacagtagca cggatgcttg
     2941 aaagggatga tttcgagaac aggtataaag aaggtcgccc aattgggatt cacgagtttt
     3001 tctatccttt aatgcaggct tatgattcaa ttgcacttga agccgatgtt gaactcggag
     3061 gcacagatca gaagttcaac aacctgatgg ggcgcaccct ccagcgggaa tacgggcagg
     3121 atccccagtg tgtggtgctt acgcctcttc tccctgggct tgacggcgtg aacaagatga
     3181 gtaagagctt gggcaattac atagggattg atgaggagcc gtttgagatc tatggaaaga
     3241 ccatgtcaat tccggacgag ctgattgtgg catacgtcga ttcggctacg acgatgtctg
     3301 tggctgagaa aaagggaatt gaggaaggtt tggcatcagg gacgctccac ccgatgacgg
     3361 caaagcggcg cctggctttc cagcttgttc tccagtttca cggagaagat gccgctaggg
     3421 gcgcgcagga tgagtttgac agggttttca agcacggcga gcatcccgac gagatgccag
     3481 aagtaagagt atcacaggat gatctcgtgg atggtaagat tgccgtttcc aagctcgtgg
     3541 tcatcgctgg gttagcttcc agcaacagtg aagccagacg cttgattgag cagggtggag
     3601 tgagaattga cgatgtcagg gtagagggcg tcatggacga ggttgagcct gtaaatggca
     3661 tggtgattaa cgtgggcaag agacggtatg cgaaggtcat tagagaataa agtgcggagg
     3721 tggccaaatt gagtcttgtg gcctccaaag agattttgga cgcggccaat gagggcaggt
     3781 atgctgtagg cgctttcaac gtcaacaacc ttgaattcgc aaagggtgtt gtgagcgctg
     3841 cagaggagga acggtctcca gcaatcatcc aggtgtctga gagcgctata gcctatgcag
     3901 gcattgagga gatagcgtgc atcgtaaggt ctctggcgaa ggatgtcgga gtccccatag
     3961 cgcttcacct tgatcacgga cgcaagttcg atgttatcat gcagtgcata aggcatggct
     4021 ttacctctgt aatgatagat ggctccaatc tgccattgga tgagaacata gctatcacca
     4081 agaaggtagt ggaagctgcc cacggtgctg gagtcagcgt tgaagcggaa cttggacgat
     4141 tggctggaat agaagatgac ataaatgtcc aggcaagaga cgccttcctt accgatccag
     4201 aagaggccgc catatttgtc cgggagaccg gggtcgatgc tttggcaata gccataggca
     4261 catcgcacgg gccgaggaaa ttcaagggcg aaccagtatt ggcgctcgac ctagtgacag
     4321 agataaagga gaaagtcggc attcctctgg tgcttcatgg ggcttcaggg gtatccactg
     4381 atgtgataga gcaggcggag aaatacggcg ctaagtgggg cgggtccaag ggcgttccgg
     4441 acgagagcat agttgaagct attaatcgcg gcataaacaa ggtaaatatc gacacagata
     4501 tgaggctagc ctttgtggcg agcataaggg agacgctttc aaccagacct gaggttacgg
     4561 atcccaggga agttctgagg cctgctatgg ctgccatcaa gtctgtcgct gccggcaaga
     4621 tgaggttgtt tggaagctca gggcaggctt gagcattcga ctgtccccct ctttctgatg
     4681 ggctgaacca gtctttcagc attggtagcc tgaccactta agatggaaaa ggcgacgcag
     4741 acatgccctg agcatttggc acaaaggcca ccacacccgc atacgatggg gaggggtggt
     4801 ctttttgtgc gtgcggattc cctggggctt ccaggcgcca ggatggctta tgcgctttct
     4861 ctctgtattt acaggttccc gctcacatag agcccctact cggcggcggc attcgggaag
     4921 gggcattggc acgtcatcgg gcttgtcacg ggcaagaagc atggcgcctc ggagcggaaa
     4981 aaggggccaa ggcgtgaaca gacgaatgcg gtcggtggtg ctggttgcgc tattttgcgt
     5041 gtgcatgggc tttttgggct ttgttatcgt ggaggtgcga ctcaaaccta ccctttttga
     5101 actggctgag gctagagcta gggcggttgc gactaccgca gtcaattccg cattatctga
     5161 aactggcgcg ctcgacataa agtatgagga cctcatggat tggaagactg acggtctcgg
     5221 caacatcgtc gcagtgcagc ccaacacggg tgagataaac aggatagctg ccagcactac
     5281 tacccgggtc caggaagctc tccaagagat agagagggta aggatatccg tccctatagg
     5341 gcaggtcttc ggaagtgccc tcttggccca cgtgggaccg tggctcacga ttacagtagt
     5401 tcctatagga gtagtgagca ctactgttac agacaaattc gaaacggcgg gtataaacca
     5461 actgaggcat agaatctact tggaaatcga ggcctacgtc aagattcttg ttccgctcgt
     5521 gacatcgaac gtgagggtca tgacgagcat gcctattgca gaagcgataa tcctgggcga
     5581 ggtgcccaac gtttacgttc atgtcggcga gcctgataag gttctcaggg gcatactagg
     5641 ggggcctgga gatgagtgag aatcggcgga gggcgaaaaa tcgagaaaga cagaggataa
     5701 tcgagctgat tcaggtacaa actagcttgt caggagaagg aacgaatccg agccagtatt
     5761 ttattgtcca aaggtgttgc agagaaacgc cgcgggtgat atagttactt ttgcgatgcg
     5821 gcgctaaagc cgcggatgtt ccttgaaaac caaacagcgt gaagcttg
//
LOCUS       DAOSYL010000095         5748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_11730_length_5748_cov_7.949236, whole genome shotgun sequence.
ACCESSION   DAOSYL010000095 DAOSYL010000000
VERSION     DAOSYL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11730_length_5748_cov_7.949236"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1238..1675
                     /locus_tag="PLU15_10310"
     CDS             1238..1675
                     /locus_tag="PLU15_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO31340.1"
                     /translation="MGITRWDPFDDLVALRDAFGRVLGDTSLRTGFSGIGRWRPSVDM
                     YETNDNVVVRADLPGVDRDDLEVTVTDNTLTIKGQSRHEHEIESGNVFRRERAYGQFT
                     RTLPIDAGIRADEAKASFNQGVLEIVIPKTEESRVRTRRIDIN"
     gene            1816..2424
                     /locus_tag="PLU15_10315"
     CDS             1816..2424
                     /locus_tag="PLU15_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO31341.1"
                     /translation="MKESSETWIIHIIRDFQELRSVGVTVLTKNVIEAIEERRSVREF
                     KPDPIPDSIIARILECGLKAPSAGNVQPWQFWVVSRPSLKSALAEAAFGQESVEQAPV
                     VIVVLAEPSRSATRFGYRGSKLYCIQDTAAAVQNMMLAATAYGIGSCWVGAFDEKMVS
                     KALGLSERFRPMALVPMGYPAEVSQRRPRSLRPLAETVVTVE"
     gene            2622..3479
                     /gene="sfsA"
                     /locus_tag="PLU15_10320"
     CDS             2622..3479
                     /gene="sfsA"
                     /locus_tag="PLU15_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOO31342.1"
                     /translation="MDPRLEERHFLRPSVVYARGRVPVEAAFVARPNRFVAIVRLSCD
                     VEHAYGLDRAGAEITAHVADPGRLEELLIPGAKVYVLPAFPKGMDKMDYSPTLAGVGR
                     KTLYDLVLVDYEGILVSIDSRVPNELVHRALQAGFFPELAEYHQIVRESAFGSSRFDF
                     HLSAPGQVIGTQAAILDENISDCFVEVKSVTLVEDGLAKFPDAPTARGARHMRELAQA
                     VSAGLRGVVIFVIQRNDASNFTPNRAMDPKFAGALSDSVEAGVEVWAWRCDVGLDRIS
                     LDAEVPVNI"
     gene            3741..4715
                     /locus_tag="PLU15_10325"
     CDS             3741..4715
                     /locus_tag="PLU15_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31343.1"
                     /translation="MDITRNALAVESLTKTFRDGGRGSVKAVDNVAFQVEQGEVVGLL
                     GPNGAGKTTTIKCILGLMRPDSGHVTVFGEDAQTNYPRILSKMSAVLEGSRNLYWRLT
                     VWENILFFSALHGVASPADKRYFDHLLKLFSLEDKRNVEVRKLSQGMKQKTSVVCALA
                     RRAPLVFLDEPTLGLDVETSLELRSVLRELSCEEGRTFVVSSHDMAVIQDVCQRVIIM
                     SEGRIVVDDNVSHLMDLFRTRCYRIVLRGPAPEGLEKRLVHEFVGSSVAEGEQGAEIK
                     VNLSEDEDIYRLMDILRGVDGVIDEISHEEIDMEKVYLEVVRKERSFQ"
     gene            4712..5491
                     /locus_tag="PLU15_10330"
     CDS             4712..5491
                     /locus_tag="PLU15_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31344.1"
                     /translation="MNLGTVFGAVFRRELTMIRRYMVNSISSLVSVYLVFILMFMGFQ
                     SMSNYVKTGALEGSLEGTIVGFFVWTFTIYAFSDLSWSLISEAQAGTLEQLYLAPCGF
                     KWVGACTVISNFIFSLVPIMVLLFAMMATTGKWLNLDFVSLIPLLLITLLGAYGFGFA
                     LGGLALVFKRIQAFFQIMQFVFVGFLIVPTRLAWAKFLPLAVGNDLISTVMVDGARLW
                     ELPLNDILTATAVGAAYLAIGIVIFSCCENVAKSRGLLGHY"
BASE COUNT         1262 a         1385 c         1708 g         1393 t
ORIGIN      
        1 tatagacgtg ctatgaacgg ccaatagtaa aaagcaaatc tgcaccttga taactgaacc
       61 tgctggcctc catttgccat ccggaccata tggctgattg cgaattcaaa cgaaataaaa
      121 tgaggtgaac tagggctcct gttcctagac ccgtgatttt tcatggccaa aaggggattc
      181 tcaaatggcc attgacaata tgaccctacg gagttctggc gagccccgtg gttccttgat
      241 tcgctgtcgc atcgatttct gcagatgcca ccacggtgta tgtcaccgat tttcccaatg
      301 atggccgcgg cgacagtagc ttctaggagc cccggagcat gctgcgattg cagttcctcc
      361 atggtaagcc tgatgcagga aatctgaggc tctatgaact agatctggct gaggattagt
      421 ttcaattcaa aggccatcca cagctaatca aatagctgaa ggtttctctg gcgagctctt
      481 gggtgcaccg agcgtggccc ttgagcttat tgaggtcaag cgctgcgatt gtttccggtg
      541 tagcatggcc ttttagcaac tcttgagatg tctcgatgaa gcaactggca tactcaggga
      601 agatgcgatc tagaacccga tagcgcgttg gcctccgagg ccatgaaggt ctcttcagtc
      661 tggttgagac gtagcaggtt ggcgaggatg aagggtcgat cttgctcttt gatatggcgt
      721 cagagtggat gggttaatca catggactcg gtatccctgt agcggcagcc agtaggcgcc
      781 gctaacgcgc ttgcgctcag ctttgggctg cgtgttcgga atagtcatca ttgacacaag
      841 cttcgtgctg cttcttggcg acgtcgatac cggtcaagtc agtagtagac aacctagctg
      901 tttactcgag acatgtgggt ataccgcatt cccaacgtgc tcattcgaaa atccttgtag
      961 aggcggggta atactctatc gggcttccca gtcaatgaaa ctaccaagac acgttggctt
     1021 tccgaaacat gactcctctt tggttgtcaa ggatcatctg gggcctgtac tagggaactt
     1081 cattctcttc gtgtttgagt cgcgcgcgtc ggagtaatac tacctactgc aaacatggcg
     1141 ggaagcgggc ggcgttagcc ggaggcgagc agcctttttg gtcttgggga gctttgcggg
     1201 tgcagggaaa tatgatgtgg tttgggaggg ctgatagatg ggcatcacaa gatgggatcc
     1261 attcgatgat ctcgtcgcgc ttcgcgatgc gttcggtaga gtgcttgggg atacgagcct
     1321 gaggacgggc ttctcaggga taggccgttg gcgtccttcg gtggacatgt acgagaccaa
     1381 cgacaacgtc gtagtcaggg cggatttgcc cggagtcgat cgggacgacc tcgaggtgac
     1441 cgtgacagac aataccctga caatcaaggg gcagtccagg cacgagcatg agatcgaatc
     1501 gggaaatgtc tttcggaggg agcgcgccta tggacagttc acgagaacgc ttcctataga
     1561 tgccgggatt cgggcagacg aggccaaggc cagcttcaat cagggggttc tcgagatagt
     1621 cattccaaaa acggaagagt cgagggtgag aacgcgaaga atagacatta actagcagca
     1681 acgcaatgcc gaacagaggc tacggctgcc cggcccgccc gtataatccc cctggcaatc
     1741 gcgattggag caaagtgcct caccaagcgg ataatgtaat gttttgcatg attgttgctg
     1801 catattaaag gatgtatgaa ggaatcttca gaaacatgga taatccatat tatacgtgat
     1861 ttccaggagt tgcgttcggt gggggtgact gtattgacca agaacgtaat tgaggccatc
     1921 gaggaaagac gcagcgtgag agaattcaag ccggacccta tccccgactc cataatcgcc
     1981 cgcatcctcg aatgtggact taaggcgcca agcgcaggga atgttcaacc ttggcagttc
     2041 tgggtggtca gtcggcctag tctcaagtca gctttggccg aagccgcctt cggccaagaa
     2101 tccgtggagc aagcgcctgt agtcatagtg gttttggccg agccttcgcg gagcgctact
     2161 aggtttggtt accgcgggtc taagctctat tgcatacaag atactgcagc tgcggtacag
     2221 aacatgatgt tggcggcgac agcttatgga atcgggtcct gctgggtagg ggctttcgac
     2281 gagaagatgg tgagcaaggc ccttggcctg tccgagcgat ttcggcctat ggcattggtc
     2341 cccatgggat atccggcgga ggtatcgcag cgccggccgc gttccctgag gccattggct
     2401 gagacagtcg tcacggttga gtaagacgct gtcgagcgag catcgcacgt tgaagtgcct
     2461 gatgcgctcc tactacgaga gctctttttt gcgcagaagc gtgggttaac acgaaatgca
     2521 tctacgtgtt aggagaaatc acggttttgg cgcgggttag cgtggccgat cagggcctgg
     2581 agggctaacc cgcgcttgat tttcgatggg aggagtcgat catggatcca cgattggaag
     2641 aacgccattt ccttcgaccg tcagtagttt atgctagggg ccgagtgccg gtggaagcgg
     2701 cttttgtcgc aaggcccaat agatttgtgg ctatcgttcg tctcagctgt gatgttgagc
     2761 atgcctacgg tctcgatcgc gctggcgccg agatcactgc ccacgtagcc gatcccggtc
     2821 gtcttgaaga actactcatt cccggggcca aggtatatgt gttgcctgct ttccctaagg
     2881 gaatggataa gatggattac tcaccgactc tagcaggagt gggaaggaag acactctatg
     2941 atcttgtttt ggttgactat gaaggtatat tggtatccat agattcacgc gtgcccaatg
     3001 agctcgtgca cagggctctg caggcaggct tttttcctga acttgccgag taccaccaaa
     3061 tcgtgcggga atcggctttc ggcagcagca ggtttgattt ccatctttcg gctccgggtc
     3121 aggttatagg gacccaggct gccatcttgg acgagaacat ctctgattgc tttgtagaag
     3181 tgaaatctgt gacccttgtt gaggatggct tggccaaatt ccccgacgca cccacagcca
     3241 gaggcgcgag gcacatgagg gaactcgccc aagcggtttc cgcaggcttg agaggcgtcg
     3301 tcatcttcgt catacagcgc aacgacgcgt cgaacttcac gcccaatagg gccatggacc
     3361 cgaagttcgc tggcgccttg agcgattcgg tggaggccgg ggttgaggtt tgggcatggc
     3421 ggtgcgacgt cggtttggac aggatctcgc ttgacgcaga agttcctgta aacatctgac
     3481 atcgtctctg acagctcgcc atggaagttc cctccatcca acatatgtgg tttgctgcac
     3541 aatacaggcg taatcaataa atgttactga gtgtttaata ttagggacta tagacttgac
     3601 aatattgaat acatcattta ggattgaaac ggtggaattg aaactcatgt tatccactgc
     3661 ctggcgcaga tcccaggaat aagatgcctg aaggcgcagc atgggcaggg cagccggaag
     3721 atgggggagg tggcttggtg ttggacatta cacgcaatgc tctcgcagtg gagtctttga
     3781 cgaagacgtt tcgcgacggg ggtcgtgggt cggttaaggc cgtcgacaat gtcgcttttc
     3841 aggtcgaaca aggcgaagtc gtgggcttgc tcgggccgaa cggtgcgggc aagacgacca
     3901 cgatcaagtg catcctcggg ttgatgcgtc ccgattccgg acacgtcaca gtgtttggcg
     3961 aggatgcaca gacgaattat ccgcggatac tctcgaagat gagtgcggtt cttgagggga
     4021 gccggaacct ctactggcgg ctgacggtgt gggaaaacat tctctttttc tccgcgctcc
     4081 acggggtggc atccccggca gacaagagat acttcgacca ccttctgaag ctcttttcat
     4141 tggaagacaa gcgcaatgta gaagtgagga aactgtcgca gggcatgaaa cagaagacga
     4201 gcgttgtgtg cgccctggcg cgtcgcgccc cgctggtctt tctggacgag cccactttgg
     4261 ggttggatgt cgagacatct ttggagctga ggagcgtatt gcgggaactt tcttgcgaag
     4321 agggccgaac gttcgtagta agcagccacg acatggccgt gatccaggat gtctgccagc
     4381 gcgtaatcat aatgtccgag ggccgcatag ttgtggacga taatgtgagc cacctcatgg
     4441 acctcttccg gacgaggtgt tatcggatcg ttcttcgcgg acctgcaccc gaggggctgg
     4501 agaaaaggct cgtacacgag tttgttggtt cgtctgtggc tgagggggag caaggcgccg
     4561 aaatcaaggt gaacttgtct gaagatgagg atatctaccg cctgatggac atcttgcgcg
     4621 gcgttgacgg agtcatagac gagatctctc acgaagagat cgacatggaa aaggtgtatc
     4681 tcgaggtcgt caggaaggag cggtcgtttc aatgaaccta ggcactgttt ttggtgctgt
     4741 cttcaggcgg gagttgacga tgatcaggag gtatatggtc aactccatat caagcttagt
     4801 gtcggtatac cttgtcttca tcttgatgtt catgggcttt caaagcatgt ccaattacgt
     4861 taagaccggg gcactggaag gctcccttga aggcactatc gtcgggtttt tcgtgtggac
     4921 gtttacgata tatgccttct ccgacctgtc gtggagcctc ataagcgagg cccaggcggg
     4981 caccttggaa cagctgtatc tggcgccatg cgggttcaaa tgggtggggg cctgcacggt
     5041 catatccaat ttcatcttca gcctcgttcc gataatggtt ctcctgtttg caatgatggc
     5101 cactactggc aaatggctca acctcgactt tgtcagcctc atcccactgt tgctcattac
     5161 gttgctgggc gcatacgggt tcggctttgc cctgggcggc ctggccctgg tgttcaaaag
     5221 gatacaggcg ttcttccaga tcatgcagtt tgtattcgtt ggctttctga tagtcccgac
     5281 acgcctggcg tgggctaagt tcctgccgct cgctgtggga aatgatctga taagcacagt
     5341 gatggtcgac ggcgcccgcc tatgggagct tccattgaac gacattctga ccgccactgc
     5401 agtgggcgcg gcatatcttg ccatcggaat cgtgatattc tcgtgttgtg agaacgtcgc
     5461 caagagccgt ggccttctcg gtcattattg atctttcggc gatcgattgc cggatcgagg
     5521 gcattatctc tcctgatatt ggcaaggagg gcaagcgtgc ggggaatcga agatgtctgc
     5581 gctgtccaga gactctgcaa gatttatcgt ggatctcggg cggcatccaa ctcccatatc
     5641 aacctgcgag tgttgaaagg ctaaacctgg gcgttttcgg ccttggccgg acaacggggt
     5701 gtgctccgcc ccgtcagcgg ctctacatat cttgggcaat gaaaaccc
//
LOCUS       DAOSYL010000096         5702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_11869_length_5702_cov_8.516380, whole genome shotgun sequence.
ACCESSION   DAOSYL010000096 DAOSYL010000000
VERSION     DAOSYL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11869_length_5702_cov_8.516380"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..647)
                     /gene="thiT"
                     /locus_tag="PLU15_10335"
     CDS             complement(69..647)
                     /gene="thiT"
                     /locus_tag="PLU15_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOO31345.1"
                     /translation="MSTTRSRKPTPTIVLTEVGMAAALSWVLGMIRLFKMPQGGSISL
                     EMVPILYMAFSRGVYPGLLSGLIAGVLDLVTATSEIVHPMQAILDYILASTALGLAPW
                     FSFGRGWWRNLTGAIGGTLVGLMCHVLSGHIFFGQYAPAGTPAWLYSLTYNATYYLPK
                     MIITIVVVVILMQKGVLRPENGGRGNGNRSQG"
     gene            complement(989..1192)
                     /locus_tag="PLU15_10340"
     CDS             complement(989..1192)
                     /locus_tag="PLU15_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31346.1"
                     /translation="MEVTNIKGVLVATLVMDEGDADEIGTMSESEVIRRARKAVGTVI
                     PEHLIREILVGHDGDSLHVAFSI"
     gene            complement(1954..2487)
                     /gene="spoVT"
                     /locus_tag="PLU15_10345"
     CDS             complement(1954..2487)
                     /gene="spoVT"
                     /locus_tag="PLU15_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOO31347.1"
                     /translation="MKATGIVRRIDDLGRVVVPKEIRRTLRIREGDPLEIFTDREGSV
                     ILKKYSPIADLGEFAQEYADSLYEATRHIVLIADQDEVVAVAGASKKSFYERRITEHV
                     EEVMRNRKPFLSNNANENPPIEDLELSSQVIAPIIVEGDIAGAVIMLSQEGKSMGELE
                     LKLAETAAGFLAKQLGQ"
     gene            complement(3269..3604)
                     /locus_tag="PLU15_10350"
     CDS             complement(3269..3604)
                     /locus_tag="PLU15_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOO31348.1"
                     /translation="MKLWRCGVCGYIHDGSTPPEKCPKCGAPAERFSEVDDKETELIR
                     RSRYTNDLHCRLIALMEQVEEIAEAGIDDDLDPGCVAVFKRAREEAWIIGQSAKAEIR
                     AHIAKGKWS"
     gene            complement(3656..4153)
                     /locus_tag="PLU15_10355"
     CDS             complement(3656..4153)
                     /locus_tag="PLU15_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOO31349.1"
                     /translation="MAAFAGESQARNKYTYFAKVAKKEGYEQIGGIFEETAENERAHA
                     KRLIDYVGLVGDTAQNLQHAADGENYEWTSMYPEFERVAREEGFDRIADTFREIGEVE
                     EEHEKRFKALLANVENGTVFSKGQPVKWKCRNCGYVHEGPEAPKACPACAHPQAYFEL
                     FAANY"
     gene            complement(4299..4457)
                     /locus_tag="PLU15_10360"
     CDS             complement(4299..4457)
                     /locus_tag="PLU15_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOO31350.1"
                     /translation="MKKWVCTVCGYVYDPAAGDPDNGVAKGTAFEDLPDDWVCPDCGV
                     GKELFEEE"
     gene            complement(4891..5250)
                     /gene="rnpB"
                     /locus_tag="PLU15_10365"
     ncRNA           complement(4891..5250)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLU15_10365"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5292..5474)
                     /locus_tag="PLU15_10370"
     CDS             complement(5292..5474)
                     /locus_tag="PLU15_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOO31351.1"
                     /translation="MKRRCEICGKVIPNERLEALPDTKRCIECARSCGTDVCAKRSEV
                     GMDPDTYKDLLGAVRS"
BASE COUNT         1302 a         1753 c         1231 g         1416 t
ORIGIN      
        1 ccgattcttg acgggaaaac agggttgccc cgtctgcgac cctccctgtc tcctcaaacc
       61 ttccatggtc acccttggct ccggttccca ttgccccttc cgccattctc tggacgcaag
      121 acgccttttt gcatcaggat aaccacaacc actattgtaa ttatcatctt gggcaaatag
      181 taagtagcat tgtatgtcaa cgaatagagc caagcaggcg ttcctgctgg cgcatactgt
      241 ccaaaaaaga tgtgcccgga gaggacatgg cacatcaacc cgaccagcgt tcctcctata
      301 gccccagtca aatttcgcca ccagccacgc ccgaacgaaa accaaggagc aaggccaaga
      361 gcggtcgaag ccagaatata gtcaagaata gcctgcatgg gatggacgat ctcagatgta
      421 gctgtcacaa ggtcaagcac gcctgctatt aggcccgaga gcaagcctgg atagactccc
      481 ctcgaaaaag ccatgtagag tatggggacc atttccaggg agatagaccc cccctggggc
      541 atcttgaaca gccttatcat ccccaaaacc cacgaaagcg cggcagccat gcctacctcc
      601 gtcaggacta ttgtaggagt aggcttgcgt gaacgagttg ttgacataac actgatcctc
      661 cttgtgcgcc gcgccgcggg gcactaaaca gaaaaaccgc ggtcaccata aatgatccgc
      721 agtcacatag agacttaacc gcttccctac gctggcatta cccagggcgg gagagaattc
      781 ccccgctcga tcaggttcca gggtatcggg ggtgcgtcac aaaacaaggc aagacttcgt
      841 ctaccatcaa tgcaagcctg agctcgtgca acacaccatc ccgctctcag cctgttggct
      901 cccctagcgg atcggcacag tattaagttt gaacatagcc atatatctta ttcatttgac
      961 gcgcgaacgc gaaatcctcc tgtgcccttc aaatgctgaa agccacgtgc aagctatcgc
     1021 cgtcatgccc aactagaatc tctcgtatca ggtgttcagg aattaccgtt cccactgcct
     1081 tcctagctct tcggatcact tcgctctccg acatcgttcc aatctcatca gcatcgccct
     1141 cgtccatcac caaagtggcc acgagtacac ccttgatatt agtgacctcc atacagattg
     1201 cctcctccca tgtcgaagtt gtgattcgac gcatagagga gccatcctgc ggctcacggt
     1261 cagcgatctt ccgataaaag gccgacataa ccagcgaata tcagatcatg atatcgaaga
     1321 gccgcaagat actgatgaca aaactaggat catttcagtt ccctcgaaag atcacggcaa
     1381 aacaacgatc gtccgtaccc cgcttggggc cattttgcga atatcgagtg gggtggcgcc
     1441 aggtagaggc atccctctca cataaccgta cgcacggacc tctgcttctc acttacatgg
     1501 ctgcagtttt tccagagcct tgcgaggcgt ttccagctca aagccgatct accccgcgtt
     1561 ggtcttcgtc ccttcgcaac tgcatcggct tttgactcat gcagccaggc tgctgccatc
     1621 tcagttttgt tctacagact tccgtccttg cgtccccggt caagtttgtc tgcctcaaca
     1681 cgaatccgtc cttcctaact tacccggccc tcgggccttc ccgtatcgcg caaggttgcc
     1741 tactttaaag gccaacttcg gttcgcaatc gctacgctcc gtgttcaccc ttgtaacttc
     1801 cttcagaccc caccacttta ggcagcgccc ttgtctatgg attgtcttcc cgctaaaaag
     1861 gacctttcgg gctcaataga tatgccgggc gaacacaaaa acgggatcgc aactcgggcg
     1921 atcccgcatt accctaatgc acagcctctt ctcctattgt cccaactgtt tggccaagaa
     1981 tccagccgct gtttcagcga gtttcaattc gagctcgccc atggacttgc cctcttgaga
     2041 caacatgatc acagctccgg cgatgtcccc ttcgacaatt atgggagcaa ttacctggct
     2101 agacaactcc aagtcttcga ttggcggatt ctcgttggca ttgttggaaa ggaatggctt
     2161 cctgttgcgc atgacttcct ctacgtgctc agtaatccga cgttcataga aactcttctt
     2221 tgaggctcct gctaccgcaa caacctcatc ttgatctgcg atgagcacta tgtgcctcgt
     2281 tgcttcgtac agtgaatccg cgtattcttg ggcaaactct ccaaggtccg caatggggga
     2341 atatttctta aggatcaccg atccctcgcg atcggtgaat atctcaagcg gatctccctc
     2401 gcgtattcga agggtcctac ggatctcttt gggaacgact actctgccaa ggtcatcgat
     2461 tcttcttact atccctgtcg ccttcacgca catccctccc ttgttattat tttaccttgg
     2521 cccaactcct cttatgctct ataaccaatt ccgctttcgg tcaaatctgg gtgtgttcgt
     2581 gaagactcga gcagcattat caggaaagac aggcgaaaag caaaaaggag gctcacgacg
     2641 atctgctctc cggctgctgc atcccgattt gcgactaccc aacacctttc ggcactgagc
     2701 agccggttca cggaatgcaa caacctttga tcgaccaacg tccgaacttg ggcatcatgc
     2761 tgcagtctct cgactgcaac accacacttt cgctcgtacg ctgatcttcg ctcgaccgct
     2821 gcagataggt ttccctacaa cgacgatctc atcaaccgcc gccataagtt ggccaacctg
     2881 caacgatctt agctccggcc agttgcggtc ctgcttaaag ccgcaactga cctttgctcg
     2941 acgcacaatg gacccgacac cttgcgatat gatcagatcc attgtgggtc ttcgctcgac
     3001 cgccgtaaac ctcgttagat atcatggccg attaccgtgg ttatatgcat cagtttcata
     3061 acatattacg acgtgactcg gaatcttttc cttgtggtcc ataagattcc ctagcgttac
     3121 agctcatccg gaggtaacgt aggaccggca atacacccat ggattgaacc atgccatcgc
     3181 ctgaacctcg catcccggcc tatgctcacc atttgcccgt aaaagcctgt agccgccccc
     3241 tcgagcggcc acaggacaga aacaaaggct agctccactt gcccttggct atatgagccc
     3301 ggatctcagc tttagccgac tgtccgatta tccacgcctc ttctcgtgca cgcttgaata
     3361 cagcaacaca gcctggatca agatcatcat ctattccggc ttcagctatc tcctcaacct
     3421 gttccatgag tgcgatcagc ctgcaatgaa gatcattggt ataacgggac cttctgatca
     3481 gttccgtctc cttatcatct acttcggaga atctctctgc aggagcacca cacttagggc
     3541 acttttcggg aggcgtactg ccatcatgga tatagccgca cactccgcat ctccatagct
     3601 tcatgcataa gccctcctcg cctcacatgt cgcttcttgc tcgaggacag taacatcaat
     3661 agttcgccgc aaacaactcg aaatatgcct gaggatgagc gcacgcaggg caagcctttg
     3721 gcgcttctgg accttcatga acgtaaccgc agttccggca cttccacttc acgggctggc
     3781 ccttgctgaa cactgtgcca ttctctacgt tggcgagcag ggctttaaac ctcttctcgt
     3841 gctcttcctc cacctctcca atctctcgga aggtgtcggc aattctgtca aatccctctt
     3901 cgcgggctac tcgctcaaac tccgggtaca tgcttgtcca ctcatagttt tcgccatccg
     3961 cagcatgctg cagattctgg gcagtatcac caacaagtcc aacataatcg atgagcctct
     4021 tggcatgagc cctctcattc tccgcggtct cctcgaagat tcccccgatc tgctcgtagc
     4081 cttccttctt cgctactttc gcaaaataag tatacttgtt gcgcgcctgc gactctccgg
     4141 caaacgcagc cataatgttg tcgtacgtct tggtaccctc caacttatta gccaagatca
     4201 tcatcctccc atatctgatg ctagcagacc aacaaccgcc gtactaactg aaacgcctgg
     4261 tgcacctctc aaactccaac gcttgccagt tttgcctttt actcttcttc aaacaactcc
     4321 ttgccaacac cgcaatcagg gcacacccaa tcatcgggca agtcttcaaa ggccgtgcct
     4381 ttggctacac cgttatccgg gtctccggca gcagggtcat acacatagcc acagacagtg
     4441 cacacccatt tcttcatatc cctgtccccc ttcccatgtc ctacattaac gcagtctagc
     4501 tctacgggct cacattattc gtataccatc atggggtatg ttgtcaatag tcgttccttc
     4561 caggaacccg acgctctgcc tgacacgtga gggatcttcc gccgaacagg catccacggt
     4621 ctttcgtttt tggctagggt atccgaagag caagcgctct atgattaaga cattctgcaa
     4681 gttttcctga tccaatcgca tacagaaagg tgagccacgt ggctggggat agttgatttc
     4741 ttaaatgaac gctggaaggc gcgtgtcgtt tagccgtatt gtggcccgtt caaccaatac
     4801 attttggcat cgcgggcatc gatcctgata gcacctgatc cagcccattc ttgagaaatg
     4861 gcgatgcccg gcataagccg ggcatcacat gaaacaagta gccctataag ccgagttctg
     4921 tcatgagtga tcatctatct aggacccatg ttgccatggg cctcaagcga cctaacccga
     4981 gggaagagcg ggcaactcta ttcccctcct atttggtctt gctccgagtg gggtttacct
     5041 agccgcccgg tcacccggac gctggtgcgc tcttaccgca ccgtttcacc cttaccttgc
     5101 tccgaacaac tgtcgatagc agggcggtct gatttctgtg gcactttcct tgaagtcacc
     5161 ttcactgggc gttacccagc actctgccct atggagctcg gactttcctc aggcggccaa
     5221 aaggccaccc gcgaccactc aggctacttg cgctctcaac atcatgatgc taacactaca
     5281 tccgtttctc gtcaacttcg aactgctccg agcagatcct tgtatgtgtc aggatccatg
     5341 cctacttcgc ttctcttggc gcagacgtcg gtgccacaac tcctcgcgca ctctatgcaa
     5401 cgcttggtgt ctgggagagc ttccagtctt tcattgggta ttaccttccc gcaaatctca
     5461 catctccgtt tcacaacacg gctcgcctcc aatgcagaat ctggagaatt ggcaccctct
     5521 taattatatt tctacactcc aagcaaagtt ccttttatcg atcacatgta acttactgta
     5581 tatacacgaa taggcagaca aactgtccgc ctagcgctgc atctgtaata atttggtggg
     5641 cccaccagga ttcgaacctg ggaccaatcg gttatgagcc gaccgctcta ccgctgagct
     5701 at
//
LOCUS       DAOSYL010000097         5652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_12003_length_5652_cov_8.251206, whole genome shotgun sequence.
ACCESSION   DAOSYL010000097 DAOSYL010000000
VERSION     DAOSYL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12003_length_5652_cov_8.251206"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..815
                     /locus_tag="PLU15_10375"
                     /pseudo
     CDS             636..815
                     /locus_tag="PLU15_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(993..1436)
                     /locus_tag="PLU15_10380"
     CDS             complement(993..1436)
                     /locus_tag="PLU15_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31352.1"
                     /translation="MLSTENKDFLSFSLGRQPEKKWDLALNEGIPIRLDIRTGVGEAR
                     IDLERVLATDVELRTGVGKTDLTLPATGDVKAALSGGIGETVIHIPRGVAARIRTKTG
                     IGAVQVYGDYTRINSEYISSDFNTAKNRVDLELKDGIRSIRIQDR"
     gene            2195..3355
                     /locus_tag="PLU15_10385"
     CDS             2195..3355
                     /locus_tag="PLU15_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOO31353.1"
                     /translation="MLIQCTNALLEKMGISQSELKSPEGYEQLPNASMAWHANIVNIN
                     RRKAIVLMNNATRYPVVIYRPKPKDFARMRELIREAIIVALRMEGICQAVIDKYMADA
                     GEIEFSTTANRSMVAKLNHAVYDVRTMQDLLDESTLIQRYISIRTGRLLQSLSGNGYF
                     VPIREMLEYLERYVDEGEDGICRSVLDVELYQLKIQIEVEGFDIWRRVLVPSTFSFRH
                     LHNVIQTVFDWQNYHLHMFEARKQGSKVKRIVMDDDPETLGCMDPDSCEILQDRFTAL
                     EEIFPNYDGIMYEYDFGDSWQHKITLEQIVSSDALEATYLDGRGERPPEDVGGAWGYI
                     EYMRIMADETDPEHEDMRLWAASQAERNRTPEQINNRLKHCMKGYTYSRSLL"
     gene            3456..3566
                     /locus_tag="PLU15_10390"
     CDS             3456..3566
                     /locus_tag="PLU15_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOO31354.1"
                     /translation="MKKIGRNAPCPCGSGKKYKKCCAQPNVGPIAEILVV"
     gene            3580..4386
                     /locus_tag="PLU15_10395"
     CDS             3580..4386
                     /locus_tag="PLU15_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO31355.1"
                     /translation="MSTEEIIERLDSLGIPFEKEIFLQDIEEYYSAEQLSENWFRIFD
                     VTAEGRDADFPWLAARVLWDRLAPAYNMSMEHMGDLVHGGNQYSLANDPVRACDTWME
                     VWEAIKYKCKPKSKDLKELDKEYCGVFFVTNLCQDLELEFHNAGLRDKSYFEKRIDYC
                     RDFLNRFPNEDELITHNMRRAIADSYASLGDYEQADAEFDKLAEDYPNSPWGYIGWGD
                     IYFFEKKVDYDRAKELYMRALAIAKDEYDVMAVNESLEDLIKTEQLEIPS"
     gene            complement(5056..5571)
                     /locus_tag="PLU15_10400"
     CDS             complement(5056..5571)
                     /locus_tag="PLU15_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31356.1"
                     /translation="MRKSAWLLIVLLAISSPATACTSFAVYSDMPWYGMNFDCYSTTP
                     FLVSIVQENGRGLFFMAYLWGNRYVPTVGINEAGLFASTQLHHNETIVDIDAGDDSLY
                     MYQLFADSLVSLDRVSQVREIAATRRVVDAYPYRPTHNLFADPTGQAIVVETIATNRH
                     ITRPNLESRSV"
BASE COUNT         1448 a         1357 c         1565 g         1282 t
ORIGIN      
        1 tatgtgtagt tgctcgctcc taacactgca acgtacacat atgcgtcctc gatttcgctc
       61 gaggtgggat ccactattgg cacggtcttg ccggcgcgat gcgtttgccg caatacgatg
      121 ttaagcttgc gtactcattc gcggtaatgg tcgcagaatt ggctgtactg gatcccattg
      181 gggtttgctt gcttgtactc ataccataga agctggagaa tagcggattt cttgggtttg
      241 agcttgcgat gaacctgttc ccacttcgtc atgggcctgg cactaccagc agccgcaact
      301 atctacgacc gaccatcatc actatccatc ggcctatacg ccgcctttgc cctttatcag
      361 ttgccacagg cctgaggtat ggttctgctt gggtaatccg tagacgttgc aagtgtctta
      421 atgagacggt cagcaagacg ttaattccgc actgcagttg atcgtcatcg tgcctgttgt
      481 gatattattc atgttggccg agagtcacgt ccgatataat gctgtaaaaa ggccttagcc
      541 ctgttgtggt gggtggggat gggtatggaa aggccactgg ttctctagta gaattggagc
      601 atgagcgaac caattatcta ctaggaggag aaccaatggc tacatatcag attagcatat
      661 ccgaagagca attgcatgga ctttttgatg gggaccgtgg gttagcaaac cttcttgaat
      721 cagtgcttaa ccaggtgctt gaagcgcagg ctacggagca gcttggggca gagccttatc
      781 agagaactga ggagcggacc ggatatcgca atgggcctgg agaccggccc tcactcgcaa
      841 aaggcaaccc aaaggcggca taagccaatc atagacagca gctcaactct gagcaagact
      901 taatgaagtt ctgtcgcctg attcagtgcc atcagtgact agcttctcaa agacggaaga
      961 ggagtgaagc ccaagtctat cagggtggag tgctaccggt cctggatcct aatgcttctg
     1021 atcccgtctt tcaactccag gtcaactcgg ttcttagccg tattgaagtc agaagagatg
     1081 tattcactat tgatacgcgt atagtcgccg tatacttgaa ctgcgcctat tccggttttt
     1141 gtgcggattc gggccgcaac gcctctcgga atatggataa cggtctcacc tatacctcct
     1201 gaaagagcag ccttcacatc tcctgttgca ggcaaagtca gatccgtttt ccctacaccg
     1261 gtccgtagtt caacgtcagt agccagaaca cgctcaaggt caatccgtgc ctccccgacc
     1321 ccagtgcgta tgtccagacg tatcggtatg ccttcattga gtgcaagatc ccatttcttt
     1381 tcaggctgcc tgcccagact aaaggaaagg aagtctttgt tttccgtgct gagcacaagg
     1441 cgcgccttgg ttccggtgat gctcgaatcc tgtgcattct aaccatattt gcaaagtatg
     1501 atgattcgaa tcacttagaa tcgaatagaa tgactggaaa cggctacttg ggcatcgcag
     1561 catgaggacg cagcgctacg ccagaaccta gctgcagaga gtcgagtgcc attatcgacg
     1621 agtctaccgg taaagcccat gcgcgcgttg gcatgctgca gcatgatgcg aggacggcgc
     1681 catgcaagag ccgcgcaccg attcagtctc atgcgcaatg ctgcctcggt gcttctgaga
     1741 tctgttcaat gaggttgtgg tgcctgtact cttccagtta gcgttgcgag cgcggcaacc
     1801 ccatgggaat acagttagga tgggccagtg acttggcccg tgcgtcaaca tcgatcaagc
     1861 ctggacggaa atggcttgtg cctaggtagt tgcccccggc ctcggataca tcccgggggt
     1921 tcgcggactt tggtccgggg gaatgtcaga tgagcggtca acttcaccac ttaccggctt
     1981 tgttttactg gctctaatgt aggcgtgtgc gaaaagagag aatagactga gatggaatgt
     2041 ggacaaagcg gcttgctgac gcaggaagag ggaaggccga ggagaaattg tataattcct
     2101 aagagattga ttatagcaag aaagacagag tgaacgggtt ggaagaaaac gcccggaacg
     2161 aacagttgcc cattttgatc ggggagcgat gtacatgctt atccaatgca caaacgcttt
     2221 gctggagaag atgggaatca gccaaagcga gctgaaatcg ccggaaggtt acgaacagtt
     2281 gcccaatgca tcgatggcct ggcacgccaa catagtcaac ataaacagaa gaaaagccat
     2341 tgttttgatg aacaatgcaa caagatatcc ggtggtcata tacaggccga agccaaagga
     2401 ttttgccaga atgagagagc tcattcggga ggccattatc gtagcattgc gcatggaagg
     2461 catctgtcag gccgttattg acaaatacat ggcggatgca ggagaaatcg agttttcaac
     2521 aacggcaaac aggagcatgg tcgccaaact gaatcatgcc gtctatgacg taaggaccat
     2581 gcaggatctt ctcgatgaaa gcacattgat ccaaaggtac atcagcattc ggacgggacg
     2641 acttcttcag agcttgtcgg gaaatggcta ttttgtgcct atcagggaga tgcttgagta
     2701 cctcgaaaga tatgtcgatg aaggtgagga cggaatctgc cgatcagtac tggacgtaga
     2761 gctatatcaa ctgaagatac agattgaagt agaagggttt gatatatgga ggagagtcct
     2821 ggtgccatcg accttctcct tcaggcatct gcacaatgtc attcagacgg tctttgactg
     2881 gcagaactat cacctgcaca tgtttgaagc cagaaaacag gggtcaaagg taaagcggat
     2941 cgtaatggac gatgatccgg aaacgttagg atgtatggac cctgactcct gtgaaatcct
     3001 gcaggatcgg tttacggcac ttgaagagat tttcccaaac tatgatggaa tcatgtacga
     3061 atacgatttt ggcgattcat ggcagcataa gataacactt gagcagatag tgagctctga
     3121 cgcacttgag gcgacctacc tggacggaag aggcgaaagg cctcccgagg atgtaggcgg
     3181 agcctgggga tatatagagt acatgcggat catggcagac gagacggatc ccgagcatga
     3241 ggacatgaga ctctgggcag cgagccaggc ggagagaaac cggacccccg aacagataaa
     3301 caacagactc aagcattgca tgaagggata tacgtattcg cgatcgttgt tgtaaagata
     3361 ctccttttgt cgcagcctta gctcccctct cgtcggtgag cagatcgatt ggcctggtta
     3421 tgtccttaaa ctcggactat gtggagggat agccattgaa gaagatagga agaaacgccc
     3481 cctgtccttg cgggagcggc aagaaataca agaaatgttg tgcccagccc aacgttggtc
     3541 cgattgcaga gattttggtc gtatgaggaa gtagaagaaa tgagtacaga ggagatcatc
     3601 gagcggctgg acagtctcgg cataccattc gaaaaggaga tcttcctgca agacatcgag
     3661 gaatactact ctgctgagca actctcagag aactggttca gaatcttcga cgtaactgcg
     3721 gaaggccggg atgcagactt cccctggctg gctgcacggg ttttgtggga caggctggcg
     3781 ccagcctata atatgtcgat ggagcacatg ggcgacttgg tgcatggggg caaccagtat
     3841 tcattggcca atgatcccgt aagagcctgc gatacctgga tggaggtttg ggaagcgata
     3901 aagtacaaat gcaagccgaa gtccaaagac ttgaaggagc ttgataagga gtattgtgga
     3961 gtattcttcg tcaccaatct atgccaggac ctggagcttg aatttcataa cgccgggcta
     4021 cgtgacaagt cctattttga aaagcgaatc gattattgca gggactttct aaaccgtttt
     4081 cccaacgaag acgagttgat cactcacaac atgagacgcg ccattgcaga ctcatatgcg
     4141 agcttgggcg attatgagca agctgatgca gagtttgaca agttggcgga agattatccg
     4201 aatagcccgt ggggatatat tgggtgggga gatatctact tctttgaaaa gaaagttgat
     4261 tacgataggg caaaggagct ctatatgaga gctttggcca ttgcgaaaga cgaatacgat
     4321 gtcatggcag tcaatgagag cctggaagac ttgatcaaaa ctgaacagct ggagatacca
     4381 tcctagagct cgttaactgc ttccgggaga gccttctcag gtctgcggcc aacgagcgcg
     4441 cttcgccggc agccgcaagg cacgggggcc ggagttctgc acagcctcgt tgtaatggag
     4501 cgccaccccg aggacagggt gatccctgaa gaatgccgcc gggcccgcct atggcgcaac
     4561 cggcgacttt gttccctgag cgtctttgat tctcgcactg gccattcctg ggcgtcgggc
     4621 atgtcccgct acatggcggg gaacaaccag gcatcatcgt cggaggcgat caacgtgggg
     4681 caggcctggc aatctggggc gggttgaccg cggaccgaca atccgcccga agtatcgggc
     4741 atcaaccgcc tgccgatcat gcccatgctg ctttggctgg gtcacgaccc cgactgcatc
     4801 ctcagaaact acgtcagcac gagacgggca gaataaacac gtggcacgac acgaccgatc
     4861 ccgacacggc cttggacctt catgaagacc gattcggccc ggagttcggc gagccccgcg
     4921 cccacatttt ctattgtttg cacagcccga cctaaaggtc gcggctgatg ctgcgtttta
     4981 cgtagccttc cagaaggaag atcgaaggat atacgtggcc cacaatcccc cgatgccgag
     5041 aagtcagtgc tcttgtcaga cggaacggct ctcaaggttt gggcgcgtga tatggcggtt
     5101 agtagcgatc gtctccacaa cgatggcttg cccggtagga tccgcgaaaa gattgtgagt
     5161 aggcctgtag gggtaggcat cgactacccg ccgtgtggcg gcaatctccc gcacttgact
     5221 tacccgatcc aggctcacca gtgaatccgc aaagagctgg tacatgtaaa gagagtcatc
     5281 gcccgcatca atatcgacga tcgtctcatt gtggtgtagt tgagttgacg cgaacagacc
     5341 tgcctcgtta atccccacgg tgggaacgta acggttgccc caaagataag ccatgaagaa
     5401 gagtcccctg ccattttcct gaacgattga aaccaggaat ggggttgttg aatagcagtc
     5461 gaaattcatg ccgtaccatg gcatatccga gtagaccgcg aaactggtgc aggccgttgc
     5521 cggcgaagat atggcgagaa ggacgatcaa caaccatgct gatttcctca agaacgactt
     5581 ccccttgcgt caaaaagtct ttcatatctg ctcgacatgg gaacaagacc aactcaacgg
     5641 aatactccct gt
//
LOCUS       DAOSYL010000098         5294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_13018_length_5294_cov_10.696316, whole genome shotgun
            sequence.
ACCESSION   DAOSYL010000098 DAOSYL010000000
VERSION     DAOSYL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13018_length_5294_cov_10.696316"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..151
                     /locus_tag="PLU15_10405"
     tRNA            75..151
                     /locus_tag="PLU15_10405"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Met,seq:cat)
     gene            182..258
                     /locus_tag="PLU15_10410"
     tRNA            182..258
                     /locus_tag="PLU15_10410"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Met,seq:cat)
     gene            287..363
                     /locus_tag="PLU15_10415"
     tRNA            287..363
                     /locus_tag="PLU15_10415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:321..323,aa:Thr,seq:ggt)
     gene            1420..1569
                     /gene="rpmG"
                     /locus_tag="PLU15_10420"
     CDS             1420..1569
                     /gene="rpmG"
                     /locus_tag="PLU15_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOO31357.1"
                     /translation="MREGITLQCSECKNRNYQTAKNKRNDPDRIELKKYCKFCRAHTL
                     HKETR"
     gene            1611..1850
                     /gene="secE"
                     /locus_tag="PLU15_10425"
     CDS             1611..1850
                     /gene="secE"
                     /locus_tag="PLU15_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOO31358.1"
                     /translation="MAQEAKPTLSTRMKGFFGKIGKFFREVRAEFKRVQWPSRKETWS
                     LTLAVIVYVLIVTVFLGLVDLGLSFLVEQIVKAAA"
     gene            1847..2401
                     /gene="nusG"
                     /locus_tag="PLU15_10430"
     CDS             1847..2401
                     /gene="nusG"
                     /locus_tag="PLU15_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOO31359.1"
                     /translation="MSKMGTDKIENGRGWYVVHTYSGYENKVKTNLERRVQTMEKEDK
                     IFRVLVPTEEVTEFKDGRKKVVQRKRFPGYVLVEMVMSDDSWYVVRNTPGVTGFVGSG
                     NKPIPLTEEEMHAILRQMGVEEPKVTVTFEIGETVRVISGPFENLDGIVQEVSPERGR
                     LRVQVSMFEREIPVELDFTDVEKY"
     gene            2429..2857
                     /gene="rplK"
                     /locus_tag="PLU15_10435"
     CDS             2429..2857
                     /gene="rplK"
                     /locus_tag="PLU15_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOO31360.1"
                     /translation="MAKKVTAVVKLQLNAGKASPAPPVGPVLAPTGINIVEFCKNFNA
                     KTADQVGTIIPVEVTVYDDRSYTYILKTPPVSFLIKKALGIERGSGEPDTRKVGKLTL
                     DQVRQIAEVKMPDLNANDIEAAMELVKGSARSMGVEVAEA"
     gene            2954..3664
                     /gene="rplA"
                     /locus_tag="PLU15_10440"
     CDS             2954..3664
                     /gene="rplA"
                     /locus_tag="PLU15_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOO31361.1"
                     /translation="MPKRGKKYQDSVKVIDRMTLYGPAEGVKLVREAAKANFDETIEA
                     HIRLGVDPRRSDQQVRGAVVLPYGTGKTKTVLVFATGEKAREAEEAGADYVGGEDLAQ
                     KIQGGWLDFDAAVATPDMMSVVGRLGKILGPRGLMPNPKTGTVTFEVAKAINEIKAGK
                     VEYRVDKSGIIHVVIGKASFEEGMLIANLRVLSDAIVKARPAAAKGTYIKSVALAPTM
                     GPSVRLNPQLFAQMVAEK"
     gene            3911..4462
                     /gene="rplJ"
                     /locus_tag="PLU15_10445"
     CDS             3911..4462
                     /gene="rplJ"
                     /locus_tag="PLU15_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOO31362.1"
                     /translation="MPTPKKIQTVEEIQSKLNEASCVVLTDFRGLNVAQATALRRRLT
                     EAGVEYKVYKNTLIKIAADNVGIEGLDSYLQGPTALAFSTEDPIAPAKILADFARETK
                     ILKVKAGVLDGQIIDKDRVAELAALPPKEVILGKVVGAMQGPIFGLVNVLAGPTRKLV
                     WALDAIRQQKEEAGGDPQTVQAQ"
     gene            4481..4861
                     /gene="rplL"
                     /locus_tag="PLU15_10450"
     CDS             4481..4861
                     /gene="rplL"
                     /locus_tag="PLU15_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOO31363.1"
                     /translation="MTKEEILSAIKNMTVLELSELVKDLEEEFGVSAAMPVAAMASAG
                     AAGAAAAPEEEEKTDFDVILVSPGEQKIPVLKVVREITGLGLKEAKELVDNAPKPIKE
                     GVKKEEAEEIKAKIADVGGQVEIK"
BASE COUNT         1310 a         1222 c         1635 g         1127 t
ORIGIN      
        1 gcgtcagcct tccaagctga atgtcgcggg ttcgagcccc gtttcccgct ccagaatcag
       61 gttataaggt ttgtcgcggg gtggagcagt ttggtagctc gtcgggctca taacccggag
      121 gtcgttggtt caaatccagc ccccgcaacc aaatttgccg tgacacggct aataagcttg
      181 tcgcggggtg gagcagtttg gtagctcgtc gggctcataa cccggaggtc gctggttcaa
      241 atccagcccc cgcaaccaaa tttcgagcgg cccgtgttgg gccagtgccc gcgtagctca
      301 gtcggtagca gcgcatgcat ggtaagcatg aggtcatcgg ttcgattccg atcgcgggct
      361 ccactttgtc agaccgcacc accaatggtg cggtcatttt gtttggccgg catgtttgtt
      421 gagccgatgg actgaaagaa gtcctgtcac ccaaccagcg ggaagacaac ctcaaggcaa
      481 gggcgtcgct ggtaacggcg gggtctgaag gaagctttaa gggggaacac ggaacgtagc
      541 gaaagcgaac cgaagttggc ctgtcaggtg ggtaaccttg cgaagtatgg gaaagcccga
      601 aagctggata acctgggagc cttgaacgga cggttgaggc agacaaactc aaccggggaa
      661 gctctgcatc atccagagag ctggcaggcc taaagaagcg gagacgcaag gatggaagta
      721 gggcggtaga tgagtctgta ggattcagcc tgtgaaaagc tggcaaaagg ctggaggata
      781 aggctgaggg gacagcgatc cggagctgct gggtcctgtg cccgctcaaa gccgggcggg
      841 gtagattgcc ttcaagctaa aaacgccttg cgggactgtg gatataatca gccatgcaag
      901 aaaggaacaa aggaatcgtg gggatgaagg agctcaccaa ctacgtggtc ggtgcattgc
      961 atcttttcac ttttcaatgg aacgaagtga ctaaggcttg ttaggaaccg tgtatgagac
     1021 ctgcaggcgt ggttctttga gagggatgac gctaggcgga ttgcctggca ccatgatact
     1081 tcagcgtgaa tgatctgccc cgttcatgtc gatgcaggtg cactggctgc acattagtca
     1141 ccctagcctg ctatgaaatg cataggcgtt gcctgctgtc agcatgaccg ggtggttggc
     1201 gcttgcagct tactttcagt gtgggctgca atgtccgcat tcatcttggg cacggcagac
     1261 tatcggcgct aggcggcgtt tcgggtggat tgacagtgct ggtggggtat gataactttt
     1321 aggggtgatt atgggcgctc ctgacggggg cgtcataggt gtctgctcga gccttcgggc
     1381 gacagctgat gcaactgagt aatggggagg tctaacgaga tgcgggaggg aatcaccctt
     1441 cagtgtagtg aatgcaagaa ccgcaactac cagaccgcca agaacaagcg gaatgaccct
     1501 gataggatcg agctgaagaa gtactgcaag ttctgtaggg cgcacacttt acacaaggaa
     1561 acacgctagg gtataatcta gccgtgtttc acaggggagg gatctatttc atggctcaag
     1621 aggccaagcc gactctctcc acgcgcatga aaggattctt cggcaagatt gggaagttct
     1681 ttcgggaggt tcgtgctgaa ttcaagcgag ttcagtggcc tagccgaaag gaaacgtggt
     1741 ctttgacttt ggccgtgatc gtgtatgtgc tcattgtaac tgtgtttctg ggcctagtcg
     1801 acctgggact atccttcctg gttgaacaga ttgtcaaggc agctgcatga gcaaaatggg
     1861 tactgacaag atcgagaacg gcagaggctg gtacgtggtg catacgtact caggctatga
     1921 aaacaaagtg aagaccaatc tggaacggcg cgtgcagacc atggagaaag aggacaagat
     1981 cttccgggtg ctcgtcccca ccgaagaagt aactgagttc aaggatgggc gtaaaaaggt
     2041 tgtccagcga aagaggtttc ccggttatgt cctggtcgag atggtgatga gtgacgattc
     2101 ttggtatgtc gtcagaaaca cgcccggagt cacgggtttt gtgggctccg gcaacaagcc
     2161 gattccactt accgaagagg aaatgcatgc gatcctccgt cagatggggg tggaggagcc
     2221 caaggtcacg gtgaccttcg agattggcga gaccgtcagg gtgatctctg gtccttttga
     2281 gaacctcgac ggaattgtcc aggaggtcag ccccgaaagg ggcagattgc gcgtacaggt
     2341 atctatgttc gaacgcgaaa tcccggtgga actagatttt actgatgtcg agaaatacta
     2401 gcactcgccg gggaggaggt gtcagccaat ggcgaaaaaa gtcacggcag tagtcaagct
     2461 gcagttgaat gcgggaaaag ctagccccgc accgcctgtc ggtcctgtgc tggcgccgac
     2521 cggaattaac attgttgagt tttgcaagaa cttcaacgcc aagactgcag atcaggtagg
     2581 tacgataata ccagtagagg tcacagtgta cgacgatagg tcatacacct acatcctcaa
     2641 gacgcctccg gtgtctttct tgatcaagaa ggcgcttggg attgagagag gttcgggcga
     2701 acccgacact cgaaaggtcg gcaagctgac cctggatcag gttaggcaga ttgcagaagt
     2761 aaagatgcct gacctcaatg caaatgatat cgaagctgcc atggaactag tgaagggcag
     2821 cgccagaagt atgggcgtgg aggttgcaga ggcctaggcc ttacaaccta tccgcaacag
     2881 cctgcgcttg aatgttgcgg gctacaatcg tgggaggaca tgttccgctg gcaccacgtg
     2941 ggaggtaaaa cctatgccaa agagaggcaa gaagtatcag gattccgtta aagtcatcga
     3001 taggatgact ctgtatggac cggccgaagg cgtgaaactc gttcgcgagg cggccaaggc
     3061 caatttcgat gagactatcg aagcccacat cagattgggt gtggatcccc gtcgttcgga
     3121 tcagcaagtt cgtggggcgg tcgtgcttcc gtacggaact ggcaagacca agactgtgct
     3181 ggttttcgca acgggcgaaa aggcgcgcga ggcagaagag gctggcgccg attatgttgg
     3241 cggggaagat ctggctcaga aaatacaggg tggatggctc gatttcgatg ctgccgtagc
     3301 tactccagac atgatgagtg tcgtgggaag gctaggtaag atccttggcc caaggggctt
     3361 gatgccgaac ccgaaaacag gaacggtcac gttcgaagtg gccaaggcga tcaacgagat
     3421 caaagcgggt aaggttgaat acagggtcga caagagtggc attatccacg ttgtcatcgg
     3481 caaggcctca tttgaagagg ggatgctgat cgcgaacctc agggtgttgt cggacgccat
     3541 agtgaaagct cgtccggcag ccgccaaggg gacctacatc aaaagcgtcg cgctggcgcc
     3601 gacgatgggt ccgtcggttc gtctcaatcc ccagctgttt gcgcagatgg tggccgaaaa
     3661 gtagagttgt ctgctttgac aatagagaga tgtgcctggc ccaagacagc aggcgtcgcg
     3721 tagtgtggca acgctgcacg aaacgcggcc taagtgacga aatgtcagcc cgccgaggct
     3781 ggagggaagc ttggcttccg aacgcaaata tatgtctgtg atttgcggga cctccggcca
     3841 tacctgagcc gaggtccctt tctattgcca gtactaagcg aatcgtacgc gtgcgaacgg
     3901 aggtgtgaaa gtgcctacac cgaaaaagat ccagactgtt gaagaaatcc aatcaaaact
     3961 gaacgaagcg agctgcgttg tgctgactga cttccgtggt ctcaatgtgg cgcaggcgac
     4021 tgcgctcaga cgaaggctta cggaggcagg cgtggagtac aaggtttaca agaatacgct
     4081 catcaagatt gcagcggata acgttggcat cgaagggcta gattcatatc ttcagggccc
     4141 gactgcgctt gccttttcca ctgaggatcc gatcgctccg gccaagatcc ttgccgattt
     4201 tgcccgtgag acgaagatcc tcaaggtcaa ggcgggtgtt ttggacggac agatcattga
     4261 caaggatcgg gtcgcggagc ttgcagcctt gcctcccaag gaagtcatcc ttggcaaggt
     4321 ggtcggggca atgcagggac cgatttttgg cctggtcaat gtgcttgctg gtcccacgcg
     4381 caaactagta tgggcgttgg atgccataag gcaacaaaag gaagaagccg gcggagatcc
     4441 tcagaccgtt caggctcaat aagagggagg aaaatgtgct atgactaagg aagaaatcct
     4501 ttcagctatt aaaaacatga cagttctcga gctgtccgaa ttggtcaagg acctcgagga
     4561 agagtttggt gtaagcgcag caatgccggt ggccgctatg gcgtcggcag gagcggcagg
     4621 cgccgctgct gctccggaag aggaagagaa gacagacttc gatgtaattc tggtttctcc
     4681 gggagagcag aagattcctg tgctcaaggt agtcagggag atcactgggc tcggccttaa
     4741 agaggccaag gagctcgttg acaatgcgcc taagcccatc aaagaaggcg taaagaagga
     4801 agaagcggaa gagatcaaag ccaagattgc cgatgtcgga ggtcaggtcg agatcaagta
     4861 gtctgctgag ccttgaagtc actgaaaaag tcgaatctat gccgggggaa ggcaatgcgg
     4921 acatcgcaca cgccgactcc cggcgattct atctgtgcca gtttcaggct gtggatattg
     4981 agcaggctcg tgtttcgcca aagcttcctc ccaaccggtc ctagcgcagc cttgcgtgtc
     5041 tcttttcccc gcaataatca ctaattccat tctcaggttc gcatcgggta tcctcggaga
     5101 gttgtaagag aaactcaact tcacagtaaa catattatgc tctctggagc aatgacgtta
     5161 tgccaaaagc tacaggcggg aagaaaggga gtgcgttgta ttccaaatgt gggttttgtg
     5221 atccaagaat ggctgtaagg gctctagctg gcatgatttg acgcgattcg ggctatatgt
     5281 taacataaag gatg
//
LOCUS       DAOSYL010000099         4731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_14946_length_4731_cov_9.179855, whole genome shotgun sequence.
ACCESSION   DAOSYL010000099 DAOSYL010000000
VERSION     DAOSYL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14946_length_4731_cov_9.179855"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..3134)
                     /locus_tag="PLU15_10455"
     CDS             complement(6..3134)
                     /locus_tag="PLU15_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOO31364.1"
                     /translation="MRPIRLSMQAFGPYAGEEVVDFRELEGRSFFLIHGPTGSGKTAI
                     LDAICFALYGKTSGAERDGSDMRSHYADDSTPTVVIFDFAVGQKKYRVQRSPSYEVAG
                     RKTPIGPDATLWDRTGIDSEDEVGDEGRVLATGVKSVTLEVEEILGFKADQFRQVVLL
                     PQGQFRQALTADSAERRGIMEILFGTEPYRQIEAALKDEARSLQDKLEQAKDRRDTLL
                     KQTDAESVDELKAACEEMCEKCAMAKRTEEEMKEKAGKAAGALEKGRADAGKIEEASA
                     AAAAVAELKKREAEIAAMRDERDAARRALACASAEGLYAAAEETVEAGIKAVEDANRK
                     LAEAKELAEKAREKLEEEKSPKRAEERENAGKVVMRLESLGDKVVAIDEARSAAAEAS
                     LAARSAAGLAKEAGRDKDKLNSKLKEAKSQLKRVQKEAPEALRWETVARETRQTLQRL
                     QRRRELEADLEKANEEALEAADAAENAEKALAIEKKDLERLQDSWMAGQAAALAMSLK
                     PGMPCPVCGSLAHPKPACVAMEGPDGVGSAQDAEVVLSQDAIEAARERVRLAEEDYRR
                     LQRIAADAAQVVAEVKGRLGAWEVGIEPKEGESLSQQVQRIGAELAEAEKRAEEAEAA
                     AKRADSLDQQINDMEEQLVRAEEELAQAEKARLEAEARLAAAKARVEEREADVPVELR
                     APDALERALAEAETARNRLLKVLEEAEKASQAAEQALAAAKSEKGAADRAVEKAVTVK
                     KRRMGEFAARVTEAGFADISAYRASKRSEQQLDALEKAIDGFDQQMHAATVRLARAEE
                     AAAGIESPDLETLEAEANRLAQASDDAVAARAQLESEADAMQKLVKALEALSQEMAGL
                     EEEYGVVVRIAYVAGGKKPNKLGMNFETFILASMLDKVADAATRRLQVMSKGRYSLRR
                     TLDRKTARSAGGLELEVFDMYTGRERSVTTLSGGEGFMASLSLALGLADVVQAYAGGI
                     HLDTMFIDEGFGALDPESLDLALDTLIDLQRDSGRLVGVISHVPELEERIDARLEVSM
                     TETGSTTRFVVG"
     gene            complement(3131..4579)
                     /locus_tag="PLU15_10460"
     CDS             complement(3131..4579)
                     /locus_tag="PLU15_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOO31365.1"
                     /translation="MADNLVIVSDKGIGPSHVGYKEYRGFRWTASGCRVVGGCFLKFI
                     HTSDWHLGRIFYGVRLTDDQAYVLDQFVDLVRDESPDAVVIAGDIYDRTAPPVEAVQL
                     LDETLSRIVLGLGVPVILIAGNHDSPERLSFGSSLMQTQGLHIIGLPDMCAPIEISDE
                     HGPVWFVPIPYAEPAVVRDRLCAPDAHDHDTAMKAQFEAAAAEAINFATAQYGVVGTG
                     ELGEGPGKQKIAWGGMPDAGSPAMRSLFEHSAAADFSPNIVPWNVRAVAVAHAFVSGG
                     TVSESERPLSVGGAGMIDISHFLPFTYTALGHLHRPQTMSETAVRYSGSLLKYSFDEA
                     DHSKSVTVVEIDGDGTPSVRVVSLTPKRDVRKIKGRFEEVMRGPGPGESRHDYISVCL
                     TDREAIWDAMNRLRAVYPNVMQIVPSEDAMADAAESGLVAARVDHRKVTDLELFCDFY
                     SQVTGEAMSDDEKAAFVAILEKAQAEKEVAAL"
BASE COUNT          912 a         1610 c         1116 g         1093 t
ORIGIN      
        1 gcacttcacc ccaccacaaa tcttgtcgtg cttccagtct ccgtcatgct cacctcgagt
       61 ctggcgtcta tcctctcctc aagctcagga acgtgcgaga tcacgcctac aagcctcccg
      121 ctgtcgcgct gcagatcgat cagggtgtcg agagcaagat cgagcgattc agggtcaaga
      181 gcgccaaagc cttcatctat gaacatcgtg tccaggtgaa tccctccagc ataggcctgg
      241 actacatcgg ccagtcccaa cgctagggag agcgaagcca tgaacccctc gcctccagac
      301 aacgtcgtca cactacgctc ccgtcctgtg tacatgtcga acacttccag ctcaagccct
      361 ccggcgcttc gagccgtctt tcgatcaagt gtccgcctta gactgtatct gcccttactc
      421 atgacctgaa gtcttcgggt agctgcatct gcaaccttat caagcatgga ggcgaggata
      481 aaagtctcga agttcatccc cagcttgttc ggtttcttgc cgccagctac gtaagctatg
      541 cggacaacaa ccccatattc ctcttccaag cccgccatct cctgtgacaa agcctctaaa
      601 gccttaacca gcttctgcat ggcatcggct tcgctctcca gttgggcacg ggcagccacg
      661 gcatcatcgg atgcctgcgc gaggcgattc gcctctgcct ccagggtctc aagatcggga
      721 ctctctattc cggctgcagc ttcttctgct ctggctaggc gcaccgttgc tgcatgcatc
      781 tgctgatcga aaccgtcgat agccttctcc aaagcatcaa gctgttgctc cgaccttttg
      841 gaagctctat aggccgaaat atccgcaaat ccggcctcgg ttaccctagc cgcgaactcc
      901 cccatacgcc tcttctttac cgtcaccgcc ttttcaacgg ccctgtcggc cgcgcccttc
      961 tcggacttcg cagcagccaa tgcctgctct gccgcctgcg aggccttctc ggcttcttcc
     1021 agcaccttga gcaagcgatt gcgagcggtt tcggcctcag ccaatgcccg ttccaacgcg
     1081 tcaggggcgc gcagttctac aggaacatcg gcttccctct cctccaccct tgcctttgcg
     1141 gcagccaacc tcgcttccgc ttccaatcga gccttctcgg cctgagcgag ctcctcttcg
     1201 gcgcggacaa gctgctcctc catatcgttg atttgctgat ccagcgaatc cgcccttttt
     1261 gcagcggctt cagcctcttc ggcacgtttc tctgcctcgg ccaactcggc cccgatcctt
     1321 tgcacctgct gagagaggct ctcgccctcc ttcggctcaa tgccgacttc ccaagccccg
     1381 aggcggccct tcacttccgc tacgacctgg gctgcatctg cagcgatcct ctggaggcga
     1441 cggtagtctt cctctgctaa cctaactcgc tccctggccg cctcaatcgc gtcctgagac
     1501 aaaacaacct ctgcatcttg tgcacttcct acgccgtctg ggccttccat cgcaacacaa
     1561 gccggcttcg gatgtgcgag ggatccgcac acagggcaag gcatccccgg tttcaaagac
     1621 atggccagcg cagctgcctg gcccgccatc caggaatcct gcagcctctc gaggtccttc
     1681 ttctctattg ccaacgcctt ttcagcattc tctgccgcgt cagctgcctc aagcgcctcc
     1741 tcattcgctt tctcgagatc agcctcgagc tctctcctcc tctgcaacct ttggagggtc
     1801 tgcctcgtct cccttgcaac agtctcccag cgtaaagcct caggtgcctc cttctgcacg
     1861 cgcttcagct ggctcttggc ctccttgagc ttgctgttaa gcttgtcttt gtctctacct
     1921 gcctctttcg ccagcccagc tgcgcttctc gccgccagcg acgcctctgc tgctgcactc
     1981 ctcgcctcgt cgatcgcaac taccttgtct cccagcgatt cgaggcgcat gacgaccttc
     2041 ccagcattct cgcgttcctc ggccctttta ggcgacttct cttcttcaag cttctctcgg
     2101 gctttttcgg caagctcttt tgcttcggcc aacttcctgt tggcatcctc gaccgctttg
     2161 atgccagcct ccaccgtctc ttcggctgcc gcgtacaaac cttcagcgct ggcgcatgcc
     2221 agcgcgcgcc tggccgcatc acgttcgtcc cgcatagcgg cgatctccgc ttccctcttc
     2281 ttcaactcgg cgacagccgc agctgcagca gaggcctcct cgatctttcc tgcatccgcc
     2341 cgcccctttt cgagagcccc tgcggccttg cccgccttct ccttcatctc ctcttcagtc
     2401 cgcttggcca ttgcgcactt ctcgcacatc tcttcgcaag ccgccttcag ctcgtccacg
     2461 gactctgcgt ccgtctgctt cagaagagta tcgcgccgat ccttcgcctg ttcaagcttg
     2521 tcctgaagag acctagcttc atccttcagt gctgcctcaa tctgtctgta aggctcggtc
     2581 ccaaaaagta tctccatgat tcccctgcgt tcagccgaat cagcagtgag cgcctgcctg
     2641 aattggccct gtggcaagag gactacctga cgaaattggt cggccttaaa gcccaggatc
     2701 tcctcgacct cgagggtgac tgactttact cctgtggcca atacccgtcc ctcatcacca
     2761 acctcgtctt cactgtcgat acctgtcctg tcccaaaggg tggcatcggg ccctatcggg
     2821 gttttcctcc cggccacctc gtatgagggg ctcctctgca cccggtattt tttctggccc
     2881 acggcgaaat cgaagatgac cacagtcggc gtcgaatcat cggcataatg ggatctcata
     2941 tcgctgccgt cgcgctcggc tccgctcgtc ttgccgtaaa gagcaaaaca gatagcgtcc
     3001 aagatggcgg tttttccgga tccagtcggg ccgtgaatca ggaaaaagct cctgccctca
     3061 agctcgcgaa agtccacgac ctcttcgccc gcataaggcc cgaatgcttg catcgacaga
     3121 cgaatgggtc tcataacgca gccacctcct tctctgcctg agctttctcg agaatagcca
     3181 cgaaggccgc cttctcgtca tccgacatcg cctccccggt aacctgcgaa tagaaatcgc
     3241 agaagagctc caggtcggtg actttcctgt gatccaccct tgcagcaacg aggccagatt
     3301 cggccgcatc tgccatagca tcctcgctag gcacgatctg catgacattg gggtagacag
     3361 cccgcaaccg gttcatggca tcccatatcg cctcccggtc ggtaaggcag actgatatgt
     3421 aatcatgtcg cgattcgccg ggccccggcc cgcgcatgac ttcttcaaac cgtcctttga
     3481 tcttgcgcac atcgcgtttt ggcgtcagcg acaccactct cactgacggt gtcccatcgc
     3541 cgtctatctc cacaactgtg acagattttg agtggtctgc ctcgtcaaat gagtatttga
     3601 gcaaagagcc ggagtacctg actgccgttt cagacatggt ttggggacga tgcaagtggc
     3661 caagggctgt gtatgtaaag ggaagaaaat gagatatgtc gatcatgccc gcgcctccaa
     3721 cggaaagtgg ccgttccgac tctgacactg tcccgccgct aacaaatgcg tgggcaacgg
     3781 caacggcgcg aacattccat gggacaatgt ttgggctaaa atccgcggca gcggaatgct
     3841 caaaaagtga ccgcatcgct ggagagccag cgtccggcat tcctccccat gcaatcttct
     3901 gcttcccagg cccttctccg agctcccctg ttcccacaac accatactga gctgttgcaa
     3961 aattgatcgc ctcagcagct gccgcctcga actgcgcctt cattgcagta tcatgatcat
     4021 gcgcatccgg agcgcaaagc ctgtcgcgca caacagcagg ctcggcatag ggaataggca
     4081 cgaaccacac cggcccatgc tcgtcgctga tctcgatggg ggcgcacatg tcaggcagcc
     4141 cgataatgtg tagtccttgc gtctgcatga gcgaagaacc gaagctgagg cgttcgggcg
     4201 aatcgtgatt tccggcaatg aggatgacag gcaccccaag tcccagcact atccgggaca
     4261 atgtctcgtc gagaagttgc actgcctcca ccggaggcgc agtcctgtcg tatatgtcgc
     4321 ctgcaatcac caccgcgtcc ggcgactcat cccgaaccaa gtccacgaat tgatcgagga
     4381 cataagcctg atcgtcagtg aggcgaactc catagaagat ccgccccaaa tgccaatcag
     4441 atgtatgaat gaacttcaaa aaacatcccc ccacaacccg acagcccgac gccgtccagc
     4501 ggaaacctcg atattcctta taccctacat gcgagggccc aattcccttg tcagacacaa
     4561 ttacaaggtt atccgccaaa cccaagactc cttccccggc tacaactttc ctcgaccaca
     4621 atgtgcctgc atcagacggc gagcatccat tgcggttccg gcgcggcgag gcaacgcgct
     4681 tgggagataa gtcctagtca aagttgatta tttgatgtta tatgttttgg a
//
LOCUS       DAOSYL010000100         4602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_15415_length_4602_cov_4.784913, whole genome shotgun sequence.
ACCESSION   DAOSYL010000100 DAOSYL010000000
VERSION     DAOSYL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15415_length_4602_cov_4.784913"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLU15_10465"
     CDS             <1..239
                     /locus_tag="PLU15_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31366.1"
                     /translation="GHQRFTAAHEYFHLLYDEGMLGAACKVGVVDQPTLREAEADAFA
                     LCQVRELARALWRAQAASAVLPWRMSSTWSSISE"
     gene            655..816
                     /locus_tag="PLU15_10470"
     CDS             655..816
                     /locus_tag="PLU15_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31367.1"
                     /translation="MGLIETADIMCHAVAQGLMSISDGENLWKRMKARRRRLPDGDFA
                     DVFRRVQDS"
     gene            complement(1320..2660)
                     /locus_tag="PLU15_10475"
     CDS             complement(1320..2660)
                     /locus_tag="PLU15_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO31368.1"
                     /translation="MTESTQADLRELRRKAQELRKGRRIEEALPFYSVIWEQHRGNAN
                     EWDGWGFAFCLKHVGHYEQSLAVCHEVEQEWPEFGPIRNVHAWCLYYLYAKKEREEIA
                     DNPQEFFGAANEIVRLTEGQWNSRMSPYILTCFKVTNYLGPRATSSAAQQVLEWTGRL
                     DPHELETNASYWTGNDGKQIRTPSDRERYYNASVKALQSLARHEEALCLCDEALTAFP
                     EIHPTFEIWMKYRKGVCLVELGRHDEAIEILRLVLRQKKEWFIRHVLAKAYLGKGDLD
                     SALRCGVEAVAIDNSTDMSKRWEVFLTIAQVLIACNEDDMAKKHAAYAAGERKRMGWG
                     TNTKLDSVCSSLGITDNDIDSFHDLKPELERYWQSVRLNLSPRGTGTVANLLPSGAGF
                     IRDDDGNNYYFNRQAFKGPKHELQPGIRVAYFIEESYDARKRCDSMEALDIQLA"
     gene            complement(2724..3563)
                     /locus_tag="PLU15_10480"
     CDS             complement(2724..3563)
                     /locus_tag="PLU15_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31369.1"
                     /translation="MTKCKEELAVKYQELEKTIGETSPELLAKTDPYGFRDYQARLRV
                     TQGKHYIGDILLAYSPNRAVYTANLAAIRDDVAASTLCEICAATGCRLAGGSRQPRQP
                     GPIMQDTPATHPPRIEQARTAADLLLKALAKSGIQAEFAGIYNHYVRLNLLFNQGVPA
                     RIDIYNTAKHQLELRFTTPPANSEDAARIGKLWAPIRTSLLGGESSSASPSQSAEAAM
                     RRADVYYKTLQKYAKCDFDFIDLAIALKDEAHARGLAFPELDEVRYDFAELERNYKAM
                     AAI"
     gene            complement(3560..>4602)
                     /locus_tag="PLU15_10485"
     CDS             complement(3560..>4602)
                     /locus_tag="PLU15_10485"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOO31370.1"
                     /translation="AFDESRVYRAFAAQKDELFAEKRPRLLRQDIVCKLDRIDTGRNY
                     RAVLWAGYSRHAQLTIANRGPISPDRRKDTGFLDQSTERHFIIEYPMERETLVFGFER
                     DVCGDASLYISTSLAILRGIKSMYKLDDSEIGMMIDADFVPSSDSPLPGSNRVVYVAL
                     YDATGNNCLRLEDIYADLDSMELFAEAYEIVASCDCDRQGCHRCLRGYESRPWASQIN
                     ASSAMMFLAYLLGEGKFRPCLAPVDEKSVPDITLEVTIRGDQVELGCNGDVQVHRFIH
                     DQNTEIFAAMNEVLLERFHSGIECVRIITRASYIRNALLQQGTVDEGKEALADLLVTL
                     SMFKNVEAVDRP"
BASE COUNT          999 a         1222 c         1254 g         1127 t
ORIGIN      
        1 ttggccacca gcgtttcact gcagcgcacg agtacttcca cctcctctat gacgagggaa
       61 tgctcggggc tgcctgcaag gttggtgtag tggaccagcc aactttacga gaggctgagg
      121 cagatgcttt tgcattatgc caggtgaggg aattggcgcg cgcgttgtgg cgcgcacagg
      181 cggcaagcgc ggtcttgcca tggaggatgt cgtctacttg gagcagcatt tcggagtgag
      241 tcacaaagca atggtcagac gccttcggaa tctagaccat ataagcgcgg accaattcga
      301 gcggatgtgg agcctgccaa tccgtcaggc agctcgaaca ctaggctacg atatagccct
      361 ttacatccca acgcacgaag acaggatcat atcatcgtac gccgagaaag cgactcggat
      421 ctgatttcgt tcggcaagta cgagcagttg atgcagcgtc acatcagcca aggggagtct
      481 ctcgaactga aaatgattgc ggagggcgat atcgctgacg agtaattcca gttgactcaa
      541 gcctctagga aacccaggcc gcaagaagca gcgatataat acgttcgctt tcgtggcggc
      601 acggtcgcaa gcaacaacct gagagatgtt agggaatact gtgaggagca tcatatgggg
      661 ctcattgaaa cggcagacat catgtgccat gcggtagctc aagggctcat gtcgatttca
      721 gacggtgaga atctttggaa gagaatgaag gctcgccgtc gcagactccc ggatggggac
      781 ttcgccgatg tgttcaggcg tgtccaggat tcttgaacga tgtatagttg accccgatag
      841 ggctcggtta tgatcaaaac cggtgaagcg gtgtcaagag atgggaaatg agctgccatg
      901 agactggcga ttgtgagata agcctgggca acccggcgcg attaaccact ttgtgactat
      961 gttctcaagg ctgatggcac cgatgtaggc gacgagggag cggtctcctg ggctaataat
     1021 ctatccgatg aagaactgga gattgtcgtg tgggcaagag gactcgtttc cgacatggcg
     1081 gtactcgaca atttactcag gggggcgggg cattgacgtt gcgagctacc gattgcacat
     1141 gtgattgaag ctaaattcca actggcacct ggagttcacc ctcaacatga tgcagtagaa
     1201 gacgagcttc tctgggcgtg gcctctagac ccgtcgcgct gagctgcaag atgtagacgc
     1261 catagcccat gatgccaata agagccagtc caagttgctt gcatatgaac cttcatccgt
     1321 caggccagct gtatatccaa agcctccatg gagtcgcagc gtttcctggc atcgtaggac
     1381 tcctcaatga aataggcgac tcggatgcca ggttgaagtt catgcttggg ccccttgaag
     1441 gcctgtctat tgaagtagta gttgttgcca tcatcgtccc tgatgaaccc ggctccagat
     1501 ggtagcaggt ttgccactgt gcctgtcccg cgggggctga ggtttagtct gacgctctgc
     1561 caatatctct caagctccgg cttgaggtca tggaagctat cgatgtcatt atcggtaatc
     1621 ccaaggctac tacagacact gtcgagcttt gtgttggtgc cccaacccat ccgtttccgt
     1681 tctcccgcgg cataggcagc atgctttttc gccatatcat cttcgttgca cgctatgagg
     1741 acctgagcga ttgtgagaaa aacctcccat cttttgctca tgtcggtgga attgtctata
     1801 gcaactgctt ctactccaca ccgaagcgcg gaatccagat cccccttgcc caaatatgcc
     1861 ttggccagaa catgcctgat aaaccactct ttcttctggc gaaggaccaa acggagaatc
     1921 tcgatcgctt catcatgccg gccaagttca acaaggcaca caccttttcg gtatttcatc
     1981 cagatctcga aagttggatg aatctcggga aaggctgtga gtgcttcatc gcagaggcat
     2041 aatgcctcct cgtggcgtgc aagggattgc agagccttca cactagcgtt gtagtaacgt
     2101 tctctgtctg acggcgttct gatttgtttg ccatcatttc cagtccaata gctcgcattg
     2161 gtctcaagct cgtgggggtc gagcctgccg gtccattcca aaacctgctg ggctgcgcta
     2221 ctagtggccc ttgggccaag atagttcgtc accttaaagc acgtgaggat gtatggagac
     2281 atgcgcgaat tccattggcc ttccgtaaga cggactattt catttgctgc cccaaaaaac
     2341 tcctgcggat tatcggcgat ttcctcgcgc tccttcttgg cgtagaggta atagaggcac
     2401 cacgcgtgca cgtttcgtat ggggccaaat tccggccatt cctgttctac ctcgtggcat
     2461 acagccaggc tctgctcata gtggccgaca tgcttaaggc agaatgcaaa gccccacccg
     2521 tcccattcgt tcgcattgcc tctgtgttgc tcccagatga cgctgtagaa tggcaaggcc
     2581 tcttcgatgc ggcgcccttt gcgcagctcc tgtgctttgc gccgcagttc gcgcagatcc
     2641 gcttgcgtcg actctgtcat tctccatacc tccgatcaat tatgggctct gctgcaaaag
     2701 cgtcacttgt ctgtcgctag ctcttaaatg gcggccatag ccttgtagtt ccgctccaat
     2761 tccgcaaagt cataacgcac ctcgtcaagt tcgggaaagg caagtccgcg cgcatgagct
     2821 tcgtccttta aggctatagc cagatctatg aaatcgaagt cgcacttggc atacttctgt
     2881 agcgtcttgt agtacacatc tgcccgcctc atggcggcct ctgcagattg agacggggaa
     2941 gcactggatg attcgccgcc tagtagactt gtgcgaatgg gtgcccacaa tttgccgatg
     3001 cgggctgcgt cttcactgtt tgcgggagga gtagtgaacc tcagctcaag ttggtgcttc
     3061 gctgtgttgt aaatgtcgat tcttgctggg actccctgat tgaataggag gttcagcctt
     3121 acatagtggt tgtagatccc ggcaaactcc gcttggatgc ccgacttcgc tagggctttc
     3181 agaagaaggt cggctgcggt cctggcctgc tctattctcg gggggtgggt ggctggcgta
     3241 tcttgcatga tcggcccagg ttgccggggt tgccttgagc ctccagccaa gcggcagcct
     3301 gtcgcagcgc agatctcaca gagagtgctt gctgcgacat catcgcgaat cgccgcgaga
     3361 ttggctgtgt atacagccct attgggagag taggccagaa gaatatcgcc gatgtaatgc
     3421 ttcccctgcg tcactctgag cctagcctgg taatcgcgga atccgtaggg atcagttttg
     3481 gccagaagtt cggggctcgt ctccccaatg gttttctcca gctcctggta tttcactgcc
     3541 agctcttctt tgcatttggt catggcctgt ccaccgcctc cacattcttg aacatgctga
     3601 gtgtcacgag caggtcagcc agagcttcct tcccctcgtc aacagtgccc tgttggagaa
     3661 gcgcattccg aatgtaactt gcgcgggtga tgatccggac gcattctatg cccgagtgga
     3721 agcgctccag taaaacctcg ttcatggccg caaagatctc cgtattctgg tcatggatga
     3781 aacgatgaac ctggacatcc ccattgcagc ccagctcgac ttggtctccc ctgatcgtca
     3841 cttcgagtgt gatgtcgggg acagactttt cgtccactgg ggcaaggcag gggcggaact
     3901 tgccttcacc taggagatat gcaagaaaca tcattgctga actcgcattt atctggcttg
     3961 cccatggacg agattcatag cccctaaggc acctgtggca accttgcctg tcgcagtcgc
     4021 agcttgccac aatctcatag gcttccgcaa aaagctccat cgaatcgaga tccgcataga
     4081 tatcctcgag tcgcagacag ttgttgccgg ttgcatcgta tagcgccacg tatacaactc
     4141 tgttagagcc cggcaagggc gaatcggaac ttgggacgaa gtcggcatct atcatcatgc
     4201 cgatttcaga atcatccagc ttgtacatgc tcttgatccc tctgaggatg gcgagggagg
     4261 tgcttatgta aagcgatgcg tcgccgcata catcccgctc aaaaccgaaa acgagcgttt
     4321 cccgttccat cggatactcg atgatgaaat gccgttctgt cgattgatcg aggaaacctg
     4381 tatcttttct tcgatcgggg ctgatcgggc ccctgtttgc gatggtgagc tgggcatggc
     4441 gtgaatatcc ggcccatagc acggctctgt aattgcgccc cgtatctatt cggtcgagtt
     4501 tgcatacgat atcttgcctg agaaggcgtg gacgcttctc cgcaaagagc tcgtcttttt
     4561 gcgcggcgaa ggcgcgatag acgcgggact cgtcgaaagc aa
//
LOCUS       DAOSYL010000101         4419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_16173_length_4419_cov_7.720898, whole genome shotgun sequence.
ACCESSION   DAOSYL010000101 DAOSYL010000000
VERSION     DAOSYL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16173_length_4419_cov_7.720898"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(430..1308)
                     /locus_tag="PLU15_10490"
     CDS             complement(430..1308)
                     /locus_tag="PLU15_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOO31371.1"
                     /translation="MAVKILSDSACDLPDEILEEYNIDTLPILVSKGEEEYLDKVTLD
                     PKTMYDEMRKGVVYKTAQIPPQMFSEKFEEIAKNNESVIYVCFSSGLSGTYNTSLIVR
                     DETKRKYPDLDIDIVDSRSASVGFGLLVYKAAKMAKEGKSKEEILKMLDFYVKHIEHI
                     FTVDNLEYLFRGGRVTRAQAFVGGLLNIKPILDIPEDGTLRPIENVRGRKKLLNRMID
                     IMEERGGNADLTHQTIGINHGDDLEGALTLKKMIEERYGCTDFVVNIIGCAIGAHAGP
                     GTLSVFFLNEKPDPEF"
     gene            complement(1590..4313)
                     /locus_tag="PLU15_10495"
     CDS             complement(1590..4313)
                     /locus_tag="PLU15_10495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO31372.1"
                     /translation="MPRYRFTWDAFDDATVIAVAGACGFDPGMVTPGDVADSARDWLT
                     LNWARPNEELVRRAKRELERTWLPDYPFLKTLVIELIESGLGPMGQPKSRSEQLAYVA
                     KCRNTTNFRRILANHMRMYGEGWSEDEVGNSIIRSWAVIDPSKQLPDSSKPFPYQVEA
                     WNRLSACSARKDSPGGFPGLLVMPTGSGKTYTAVHWLMENVVARGGRVMWVAHREGLL
                     EQAWEWFVRLSGLADNPAREDPLVIRIVSGSHCVVSQLTPDDDIMLCSVASLARHKAV
                     VEDILSDPDIFLVIDEAHHAPAKSYKDIIRYMKELKHNKLLGLTATPTRMIESERAVL
                     SQLFNNNIIYQAEFRDLVEQNYLARPIKIRVSTDIEAEAGMTEEERRHIATFDNLSEE
                     TLDRLGAMNERNDLIVSHYLGNEAKYGKTLVFATCINHAHLLRDKFRANGVNAEYVAS
                     RRLDEEAVNEDEVKAEFSSSDSGLDVLINVQKMTEGTDLPSVQTVFLARPTNSAVLMQ
                     QMVGRALRGPKVGGTPLAYLVSFEDHWEQFTDFERPFDLIPELVDPFEEAAPDIMEAD
                     EDIVDKVLDVVPSEITRAVSAALSRLPMSFSRAQVFEAVPCGWYVLTRTVPTGDDVIQ
                     IIPVHNHDKPCWDALIGYLSALTPRQAEGLNPEGVYEEFFGDCDIPRPSEGLVEQLIA
                     HRMADGDEPEWQDFAARSRIDPADIAREILEANYGPRESQAIVEERYSSALAQAVYPD
                     LREFVMAVDEAVWDLMNPDQAVRVPLGIPVFEPRPDEMMAPGPAHDLHDILHSMLVAG
                     ASILGVSELDEDGIEIVWTKRLIKGWYAMAYYNLIGEYGEGLIRVNVLLDSPDISRET
                     LEFLLWHEFLHLWLKQGHTREFRRLEMTWPTFIQCKRELDTLGERYGVSFW"
BASE COUNT          966 a         1249 c         1124 g         1080 t
ORIGIN      
        1 gactgatgaa ggcgaaggct acctcccgat caaaccgatt gaagctcccg ctgtcataga
       61 tatgggctgg atcaacgaag ggacatcggt gtatctgcag gccttcactc acccccgcgc
      121 aaaatggaga ccagccgcca tagtcatcga tcagattcag gtgggcagat aggctgccta
      181 gatggcaggt tcatgttgag cttcacctag cttgaagatc gcaaactgac cgacagccct
      241 tacgaaagtg ccccatgtgg cagaaatgta cgacggcagg cgtccagggg ccagttggat
      301 gcgatttcct gccccaacta aagcgagttg cgataggcga gtcagataat gagcgcctcc
      361 acgaggcaat ccggaggcgc ccattagaca gtatcgtttg tatatagatc agtctctgcc
      421 cacgtgggct caaaactccg gatccggctt ttcgtttaag aagaatactg atagtgttcc
      481 cggacctgca tgtgccccta ttgcacagcc tatgatgttc acgacaaaat cagtgcatcc
      541 atatctttcc tcaatcatct ttttgagcgt gagcgcgcct tccaagtcat cgccatggtt
      601 tatcccaatc gtctgatgag tgagatcagc gttccctcca cgctcctcca tgatatcgat
      661 catcctattc aggagcttct ttcttccccg cacattctcg atgggcctca acgtgccgtc
      721 ttccggtatg tctaagatgg gctttatgtt caacaacccg ccaacgaaag cttgggccct
      781 cgtgaccctt cctcccctga agaggtattc taagttgtcg acggtgaata tgtgttcgat
      841 gtgttttacg tagaaatcca acatcttgag aatttcctct ttagacttgc cttccttggc
      901 catcttggct gccttgtaaa ccaagagccc aaagcccaca gaagcagacc tgctgtcaac
      961 tatgtcaata tccagatctg gatacttgcg tttggtttca tcccttacta tcaatgaggt
     1021 attataagtc cctgacaaac ctgatgagaa gcacacgtat atgacgcttt cattattctt
     1081 ggctatttct tcgaactttt cgctgaacat ctgcggcggg atctgcgcgg tcttgtacac
     1141 tactcccttt ctcatctcgt catacattgt cttgggatcc aaagtgactt tgtccagata
     1201 ttcttcctcg cccttgctca caagtatggg taaggtgtca atattgtatt cttccagtat
     1261 ttcatcgggc aagtcgcaag cactgtcgga tagaatcttc acagccataa aactcccccc
     1321 attcttttag ttgattataa tatacaactg ccgttctgtg tgattcttgg catcatctgg
     1381 ccgtgccagt ccaatccctt ctcaggaatg ccgcctcagc ctgccaggaa catcgcacca
     1441 taacactgat taccaacatc ttgacacatg ttcgcacagt cgacaagatc atgactgcag
     1501 cttgctgcaa aaggcggctt gagcctcgga acgatggagt ggtcttgtgt tcttcattgg
     1561 ctcccgctct ccggacatcc gatcgcactc taccaaaagc taacgccgta acgctccccg
     1621 agcgtatcca actccctctt gcactggatg aacgtgggcc atgtcatctc cagtctgcgg
     1681 aattcacggg tgtggccctg tttgagccag aggtgaagga attcgtgcca gagcaaaaac
     1741 tctagggtct cccgggaaat gtccgggctg tcgagcagca catttacacg gatcaatccc
     1801 tcgccgtatt ctcctataag gttgtaatac gccatagcat accagccctt tatcagcctc
     1861 tttgtccaca caatctcgat cccgtcctca tcaagctccg aaacccccag tatcgaagcg
     1921 ccagcgacca gcatcgaatg gagtatgtca tggaggtcat gtgcgggacc gggcgccatc
     1981 atctcgtctg gtcgcggttc aaagacaggg atacctaggg gcactctgac ggcttgatct
     2041 gggttcatta agtcccacac ggcctcgtcg actgccatca caaactcccg caggtcaggg
     2101 taaacggcct gggccagcgc cgagctgtac cgttcttcca cgatggcctg ggattcccgc
     2161 ggcccatagt tggcctctag gatctcccgg gctatgtcag caggatcgat cctggaccgc
     2221 gccgcaaagt cctgccattc aggctcgtct ccgtctgcca tgcggtgggc gatcaactgc
     2281 tccacgaggc cctcggacgg acggggaatg tcgcagtcgc cgaaaaactc ttcgtatact
     2341 ccctcaggat tgaggccttc tgcctggcga ggggtgagcg ccgacagata gccgatcaac
     2401 gcgtcccagc acggcttgtc gtggttgtgg acgggtatga tctggattac gtcatcgcct
     2461 gttggcaccg tacgcgtgag cacgtaccac ccgcaaggga ctgcctcaaa tacttgggcg
     2521 cgactgaatg acatgggcaa tcgactgaga gctgctgaca cggctcgcgt gatctcggaa
     2581 ggcaccacgt ccaatacttt atccacaata tcctcgtccg cttccatgat atccggagcg
     2641 gcctcttcaa atggatctac aagctcgggg atgaggtcga atgggcgctc aaagtcggtg
     2701 aactgttccc aatggtcctc gaatgacacg aggtatgcca ggggtgttcc ccctaccttc
     2761 gggccccgca gcgcccgccc taccatctgc tgcataagca ctgcggagtt ggtgggacgc
     2821 gcaagaaaga ccgtctgaac gcttggaagg tcggtcccct ctgtcatctt ctggacgttg
     2881 atgaggacgt cgaggccgct gtccgatgag ctgaattcgg ctttcacctc gtcctcattg
     2941 acggcttctt catcaagccg cctgctcgca acgtattcgg cgttcacgcc gttggcacga
     3001 aatttgtccc tcagcaaatg ggcatggtta atgcatgttg cgaacaccag ggtcttgccg
     3061 tacttcgcct cgttgccgag gtaatggctg acaatgaggt cgtttctctc gttcatcgcg
     3121 cctaacctat ccagagtctc ctcgctgagg ttatcgaacg tggctatatg gcgtcgttct
     3181 tcctcggtca tgccggcttc tgcctcgatg tcagtggaga cgcggatctt gataggacgg
     3241 gcaagataat tctgttccac caaatctctg aactcggcct ggtagatgat gttgttgttg
     3301 aatagctgcg acagcactgc cctctctgat tcgatcatcc gggtcggcgt ggccgtaagg
     3361 cccagaagct tgttgtgctt cagttccttc atgtaccgga tgatgtcttt gtagctcttg
     3421 gctggagcat ggtgggcctc atcgatgacc aggaaaatgt ctggatcgct caagatatct
     3481 tccacaacag ccttatgccg ggcaaggctc gccacagaac acaacatgat gtcgtcgtca
     3541 ggggtgagtt gactcacaac acagtgactg cctgacacga tgcgaatgac gagcgggtcc
     3601 tcccgcgcgg ggttatcggc aaggcccgaa aggcgcacaa accactccca agcctgctcg
     3661 agaaggccct ctcggtgggc gacccacatc acccgtccac ctcgggccac cacgttctcc
     3721 atgagccagt gcactgcggt atatgtcttg ccggatcctg taggcattac gaggaggccg
     3781 ggaaaccctc cgggcgaatc cttccgggca ctgcaagcag agagcctgtt ccacgcctcc
     3841 acctggtacg ggaagggctt gctggaatca ggaagctgct ttgagggatc aatcaccgcc
     3901 caactgcgga taatgctatt tccgacctca tcctcagacc atccctcgcc gtacattctc
     3961 atatgattag cgagtattcg acggaagttt gtggtgttgc ggcacttggc gacatatgct
     4021 agttgttccg accggctttt aggctggccc attgggccaa ggcccgattc tatcaattcg
     4081 atgacgaggg tcttgagaaa cggatagtca ggaagccaag tccgctcgag ctctctcttg
     4141 gcgcggcgca cgagttcctc gttggggcga gcccaattga gtgtgagcca gtcgcgagcg
     4201 gaatcggcga catcccctgg cgtgaccatt ccaggatcga atccacatgc tcctgctaca
     4261 gcaatgacag tggcatcatc aaaagcatcc cacgtgaagc gatatctggg caaggcaatg
     4321 actcccttca gatctatgga tgcattatct cagatgggtg ataatttgag gtttccaagt
     4381 gagtttgtag aagattgatt agtagtatta ttagacatt
//
LOCUS       DAOSYL010000102         4325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_16589_length_4325_cov_7.230679, whole genome shotgun sequence.
ACCESSION   DAOSYL010000102 DAOSYL010000000
VERSION     DAOSYL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16589_length_4325_cov_7.230679"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..535)
                     /locus_tag="PLU15_10500"
     CDS             complement(5..535)
                     /locus_tag="PLU15_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31373.1"
                     /translation="MKSRSTLLVSCALSLVLLLGLATSASANSRTFIGAKYKGAVGDL
                     ALAYPGINAGLELEYRFDNGFAIAAACTAGFLKAEVDDGPTVDIGLYSAALGARYYVM
                     LGDFNPYVGISGTALIANAMGASATLPGLQATAGLEMMFNKIRFAGEVGYEAFIIDKK
                     LEEGGIVYGVSLGYCF"
     gene            1125..1832
                     /locus_tag="PLU15_10505"
     CDS             1125..1832
                     /locus_tag="PLU15_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31374.1"
                     /translation="MAVARSDLAFDTPVAMKSSGVAATGSDRPGYSYYFAYCAYALLA
                     MVMTGVSSIMMSFNRPDLYLRNLCAPLPGTSMSLQLAAGHAVFALGCWAILVVGSFVL
                     HGESLVSSGLAGLYSLNTLAFAAVSAFIGFLVGSSVKSNGAQAGAINVITLGMSFLCG
                     VFVPQSIMSKSVLSVGRFLPTYWFIRANDAIGEISSFTADSLRPIYGSILIQLGFAVA
                     VFSVTLLLSKERRLSHL"
     gene            2009..2851
                     /locus_tag="PLU15_10510"
     CDS             2009..2851
                     /locus_tag="PLU15_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOO31375.1"
                     /translation="MRTEQEMMGLILGVAAADERVRAVVMNGSRADPNAPRDIFQDYD
                     IVYYVTDIDSYAEDRSWVDIFGERMIMQMPKSDCCLVYLMQFTDGNRIDLTLVPLFEK
                     QEFSTKDKLAVALMDKDNVLPPLPSPTDEDYWVRPPSRQDYDECCNEFWWVAVYVAKG
                     LCRREILYAKWHLDRIVREMLLQMLEWEASIATDFLVSMGKCRKRLEAHLPEEQWQAL
                     LLTYADGSYEGTWNSLFAACDLFRQTAKFVASHFGYEYPGGDDQRVSKHLERLRDLCA
                     SSGV"
     gene            2990..3820
                     /locus_tag="PLU15_10515"
     CDS             2990..3820
                     /locus_tag="PLU15_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HOO31376.1"
                     /translation="MIELPEATTIAEQVCTALTGKRIESVTANHSPHKFAWFYGDPGE
                     YDNLLKGHTVENAIPCGGLVEIWAGPARILFGDGVNLRYHPAGEKAPAKHQLLIEFYD
                     SSSLSGSVQMYGGLWAFRAGQFDNPYYLVAQEKPSPLTDQFNYSYFESLLEGEKRQPL
                     SAKAFLATEQRIPGLGNGVLQDILWTARIHPKRKVAALSDEELHVLFGSVKSVLKQMA
                     AEGGRDTERDLYGCPGRYKTILSRNTVDKPCPACGSAIKKAAYMGGSVYYCEGCQGRH
                     "
     gene            complement(3970..>4325)
                     /locus_tag="PLU15_10520"
     CDS             complement(3970..>4325)
                     /locus_tag="PLU15_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31377.1"
                     /translation="LDIAAMQQGIRKEIWHGWGWSPEKRADFEKQRQEILNAAREQLQ
                     GFRIFAADVGTEPRILERLEASIMNNLYEQPSPFCDIPDRGMMLSPRWDSEAPIITEN
                     RCTVRLYGLPVHLEI"
BASE COUNT          991 a         1106 c         1252 g          976 t
ORIGIN      
        1 gagactagaa acagtatcca aggctgacgc cgtatactat tccgccttcc tccagcttct
       61 tatcgattat gaaggcttca tatccgacct cgcctgcaaa gcgtatcttg ttgaacatca
      121 tctccagacc agcagtggcc tgtagaccag ggagtgttgc cgaagcgccc attgcattgg
      181 cgatgagggc ggttccgctg atcccgacgt aagggttgaa atctccaagc atgacataat
      241 acctggctcc gagagcggca ctgtacagac caatgtcgac ggtaggcccg tcatctactt
      301 ccgccttaag gaaaccagcg gtgcatgcgg cagcgatcgc aaacccgtta tcgaacctgt
      361 attccagctc gagaccagca ttgatgccag gataagctaa ggcaaggtcg ccaacggctc
      421 ccttgtactt tgcgccgata aaggtgcggc tgtttgccga cgctgaagtg gccaatccaa
      481 gaagaaggac cagtgacagg gcacatgaca ccagtagtgt gctgcgagac ttcacttgca
      541 tgacctccca aataggtagt tggtaggatt gtctaggttc caagaatcca acaagctctt
      601 gccctgggct gatgcagacc gactgctggt ctgtccgcta gccctgcgtc aatactacat
      661 tctgcaatca cctccagaat tcacgaatta tggatgaaac agaagcagta tatgtatgcc
      721 aatgcaattc ggcaaaaggg ttcccgaacc tcccacacat gcggcctata aatcgccaga
      781 tgtgtgccag acattcccag aaaccatgta tagctggcga acgtcggaat acgtcaaata
      841 gcgcgataat tggggcagca ggattggcgg tgtgatagct caaaagactc gtctggaggt
      901 ccaccgggtg aagggcttgg attctgagct gattggaaaa acgctaccgg attcattctc
      961 tcgtgtggtg cggaggggtg gcccgagact tcatacgaac taggcagggc gattctgcaa
     1021 ctgcagagag caaagaagcg tgaattcaat gaaaggcaat tgagtgtctc attgagtgga
     1081 agacgaagaa aatacggtga cggacaattg ttagacggtc cttggtggca gtcgcccggt
     1141 ccgacctggc atttgacact ccggtcgcca tgaaatcctc tggcgtggca gcaaccggga
     1201 gcgatcgtcc gggatactcc tactactttg cgtactgtgc ctatgcgctg cttgccatgg
     1261 tcatgacggg cgtaagctcc atcatgatgt cgttcaatag gcccgatctc tacttgcgca
     1321 acttgtgcgc gccattgccg ggcacgagca tgagcctgca gttggctgcc ggacacgcgg
     1381 tgtttgccct gggttgctgg gccatactcg tggtaggcag cttcgtgctg cacggcgaaa
     1441 gcttggtgtc gtcggggtta gcagggctgt attccctcaa cacgttggca ttcgcagcgg
     1501 tgagtgcgtt cataggtttt ctcgtgggca gctccgtgaa gagtaatggc gcacaagcgg
     1561 gagcgatcaa cgtcatcact cttggcatga gcttcctgtg tggtgtgttc gtacctcagt
     1621 caatcatgag caagtcggtg ctgtcggtcg gaaggttttt gccaacgtac tggttcatcc
     1681 gggcgaacga tgccataggg gagatttcga gcttcactgc ggatagcctg cgtcccatct
     1741 atggcagcat cctgatccag ctggggttcg ctgtggccgt attctcggtg acgttgcttc
     1801 tgagcaaaga gcgaaggctg tcgcatctct agggccaagc cgcctacgca atgcaggttc
     1861 caagagactg gacgagccaa cgccgcccgg attatcgtga cgcgggtggc ggcatgttga
     1921 gacatcagtg gctgcctgat tgtgctaaga tgcttttgga gcatcacaca tgttgacgct
     1981 tcggacgcaa tgcttgggag gaactcagat gagaactgag caggagatga tgggcctgat
     2041 tctcggcgta gcggcggccg atgagcgcgt gcgcgcggtg gtcatgaatg gttcgcgcgc
     2101 agaccccaac gctccccggg atatcttcca agactacgat attgtctact acgtgacaga
     2161 catagattca tatgcagaag acagaagctg ggtcgacatc ttcggcgagc gaatgataat
     2221 gcagatgccc aagtccgatt gctgccttgt ctatctcatg cagttcacag acggaaacag
     2281 gatcgatctc actttggtgc ctctgttcga aaagcaggaa ttcagcacaa aggacaagct
     2341 tgcagtggcc ttgatggaca aggacaatgt tcttccacca ttgccttcgc ccacagatga
     2401 ggattactgg gtacgaccgc cgtcccgtca ggactatgat gagtgctgca acgagttctg
     2461 gtgggtggct gtttatgtgg cgaaggggct gtgcaggcgg gagattcttt atgccaaatg
     2521 gcacctggat agaatcgtaa gggagatgct ccttcagatg ttggagtggg aggccagcat
     2581 agcgactgac ttcttggtga gcatgggcaa gtgcaggaag cgcctcgagg cgcatttacc
     2641 agaggaacaa tggcaggctc tgttgctcac gtatgcagac ggaagctatg agggcacatg
     2701 gaattcgctg tttgcagcgt gcgatctgtt tcgtcagacg gcgaagttcg tggcttcgca
     2761 ctttggctat gaatatccgg gcggcgacga tcaacgcgtc agcaagcact tggagcggct
     2821 acgggatctc tgcgcgtctt ccggcgtgta gggaccgctc ttctggcagt tcgtgatgta
     2881 ttgactacct ccagccagcc gaagaacgcg agtccgctcg cgacattcga cttgcagaaa
     2941 ccctgggcac cgtatcgcaa tctcagcaca gcacaaggag ggatttatga tgatcgagtt
     3001 gcccgaggcc acgacaattg ccgaacaagt gtgtacagct cttacaggca agaggatcga
     3061 gagcgtcacc gcaaaccact ctccgcacaa gttcgcctgg ttttacggcg atcccggtga
     3121 atacgataat ctgctcaaag gccacaccgt tgagaatgcg attccctgcg gaggcctggt
     3181 ggagatatgg gctggccccg cgcggattct ctttggcgac ggtgttaatc tgcgctacca
     3241 tcctgcagga gagaaggcgc cggcaaagca ccagcttctc atcgaattct atgactcatc
     3301 atcactcagt ggctctgtgc agatgtatgg agggttgtgg gcgtttcgcg ccgggcagtt
     3361 cgataatcca tactacctgg tcgctcagga gaagccctcg cctctgacag atcaattcaa
     3421 ttactcttac tttgaatccc tgctggaagg ggagaagcgc caacccctat ctgcaaaggc
     3481 ctttcttgcg acagaacagc gcatacctgg gctcggaaat ggagtcttgc aggacatcct
     3541 gtggacggcc agaattcatc cgaagcgaaa agtggcggcc ctgtccgacg aagaactcca
     3601 tgtcctcttc ggctcggtca agtctgtgct caaacagatg gcagctgaag gtggcaggga
     3661 caccgagcgc gatctatatg gctgtccggg gcgatacaag acgatcctta gcagaaacac
     3721 ggtagataag ccatgcccgg cctgcgggag cgccatcaag aaggcagcat acatgggcgg
     3781 gagcgtgtac tactgtgaag ggtgccaagg caggcattga tacggtggaa tgaatgaagc
     3841 cagcggtgat cgcaatgtct tgctcaacgg aaatgcttgg cgttgtgcag aacgacaagg
     3901 cgcgcctagt cagcgggttg cagagggatt gccactggtt ctgttccctg tagggaaagg
     3961 ctgatgacgc tagatctcta aatggacagg aagtccatag agtcggactg tacacctgtt
     4021 ctcagtgatg ataggggctt ccgaatccca gcggggcgag agcatcattc ctctatctgg
     4081 gatgtcacaa aacggcgatg gctgctcata aaggttgttc attatggatg cctcaagcct
     4141 ttcaaggatc cgtggctcag ttcccacgtc cgcagcaaag atgcgaaacc cttggagttg
     4201 ctctctggca gcgttaagga tctcttgcct ttgcttttca aagtccgccc gtttttccgg
     4261 ggaccacccc catccatgcc atatctcttt tcgaataccc tgctgcatag cggcgatgtc
     4321 tagga
//
LOCUS       DAOSYL010000103         4243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_16954_length_4243_cov_8.294413, whole genome shotgun sequence.
ACCESSION   DAOSYL010000103 DAOSYL010000000
VERSION     DAOSYL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16954_length_4243_cov_8.294413"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(985..2424)
                     /locus_tag="PLU15_10525"
     CDS             complement(985..2424)
                     /locus_tag="PLU15_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO31378.1"
                     /translation="MERYSPVEAWNVLKKSFRLAYERLGMVVLMSVIWYFATFTTVFI
                     SGPTIWAIPIFAVVAAPLHMAIMYCGNLAAHREDAGLKELWQGYKKFWTRAALLGAIH
                     SAVILILWIDIQVLLGTDSSLMRFLSGVWVYLSIFVGLLFMYAYPIIVEQDTTVWKSI
                     RRAVILILDNPAYTFAVGLLAAVFFAAGVLPTIGILAGNKTAGYFMIIGLSLYAGFAS
                     IYRNLATVRALQRYDAARMSGREKLARELEVEKMAQLANVNGHSSSDGQVAITWFGHA
                     CFMIQTGAGLRILTDPFDERVGYELPAVEADVVTVSHDHFDHNCVKVVKGLPKVLTGE
                     VDETIGTARIRGIKTFHDTKSGALRGLNTVFVIETDGISICHAGDLGHIPSADTMDKI
                     GDVDVLLVPVGGTYTLDGDGALEFVRKLSPKIVVPMHYQTPALKMELDGPEFFLSQIS
                     HVEQAPGRTYVISASELPRELTAVLVNYE"
     gene            complement(2429..4093)
                     /gene="argS"
                     /locus_tag="PLU15_10530"
     CDS             complement(2429..4093)
                     /gene="argS"
                     /locus_tag="PLU15_10530"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOO31379.1"
                     /translation="MRSLEGAIRKLVDSNRLCADVLDHLSSIALEVPKDARHGEFATP
                     MALSLAGVARKAPREIASLIVEEMEQEGFSSYVERAEIAGPGFINFHLSSKWLVDTIG
                     LVRSRDEKYGCSDYGRGERILVEFVSANPVGPLNIVNARAAAVGDTLVRLLNASGYSA
                     ASEFYVNDAGRQVSVLGESVYARYMELEGVDYPFPEDGYRGEYVKDVARKAMEEHGDR
                     ISAMDSDDARHFLQEFALNYILDWQRRSLEAYEVRFDRWFSERKLRQTGAVEDMVREM
                     AEAGYTYEKDGAVWFRSSDYGDDKDRVLVKSDGLYTYVAPDLAYHRDKLARGFTKLID
                     ILGPDHHGYEGRMQAGLKAFGHPDGTLEVLILQLVTLMRDGQPVKMSKRAGEFITMDD
                     LLEEVPKDAARYFFIMRNTSSHLDFDLDLAKAQTTDNPVWYIQYAHARCCSIFRQAEA
                     EGIQPTEWEQADVSCLVHPSEIDLVRKMASFPDEVLTAALERAPSRIAKYALELAGLF
                     HSFYTDCRVISDDEAQTRARLALVDAARIVLRNVLGIMGVSAPERM"
BASE COUNT         1001 a         1270 c          997 g          975 t
ORIGIN      
        1 aggttttgca gaccagtcca atttttagac atttgcgatc gcaaagcgtt caaaactaga
       61 cggtagcggt ctaaaactta gacacccgcg cccgaaaggc gactaaataa tggacacatt
      121 ccttgaaggc aggcccaagt tgccctttca tatgagtgtg ttgaatgcat agactgccgg
      181 agcgctcaca cggatctcac ggtcgggagc gtatgcgcgt cgctctcgtg gttgtaaaga
      241 agacggttgc ctgcgagcgt cgaaccggga tttcgatcta ccctggcttt gccccgggga
      301 tcatatcccg gcgcggctga atgctgcagg cgtctatggc taaggccaag atgcgtacgt
      361 tgtcgatgta caaatgcgaa ccgattaccg cactgcgaca caggtgcaaa gacgtgtcta
      421 cgcctagcag acatcatatg gcaggctaac cgatagcgca acaactgtcg gaagttacct
      481 agtcgggtgc ctgagctgtc tttcacctca gtcgcacacg atctgaagcg ccataagtac
      541 tgcccatgga ctcatcgcag gcaggtcgct actttgcggc aatttctcag acacgttatt
      601 gccggtctat cagtcttttc gaccatgtgg cagacaaaga aatcggcgct gcgtcggacc
      661 aagatacaaa aaacaggact cgtcagcaaa gctatccagc ctctagccac gtcgcaaccg
      721 caagtaatca gtctttgaac ataggctgac caaggcctag acattttccc tcccgccgat
      781 ccaatacata ggcttatgcg tttccgtata gttgtcgatt gcctgaaacc gtctcagtat
      841 gccatagatt ccctggtcat ctctgccggc aaagtgaccg gttgtgacca catcgaaagc
      901 tgtggaaccc attctcgccc tcgattgcaa aagcgcgccg gcgatttgac gctcgttttt
      961 cttccgccac aatcctgtag acgactactc gtaattgacc aacaccgcag tcagttctcg
     1021 cgggagttct gacgcgctta tcacgtaagt ccggccgggg gcttgctcaa cgtgggaaat
     1081 ctgcgacagg aagaattcag gcccgtccag ttccatcttg agcgcaggcg tctgatagtg
     1141 catgggcact acgatcttgg gagaaagctt tctcacgaac tcaagagctc catcgccatc
     1201 caaagtgtat gtcccgccta caggcacgag caacacatca acatcgccta tcttgtccat
     1261 cgtatcggcg gaaggtatgt gccccagatc gcctgcatgg caaatggaga tcccatccgt
     1321 ctctataaca aagaccgtgt tgaggccgcg caacgcccca gacttcgtat cgtggaacgt
     1381 ctttatcccc ctaatccgcg cagtcccgat ggtctcgtcg acttcgccgg tgagaacctt
     1441 gggaagtccc ttcacaacct ttacgcagtt gtggtcaaaa tgatcatggc tcaccgtaac
     1501 cacgtcggct tcaactgcgg gaagctcgta gcccaccctt tcatcgaaag gatcagtcag
     1561 tatcctcagc ccagcgccgg tctggatcat gaagcaggca tgtccaaacc atgtaatggc
     1621 cacctgacca tctgaagacg aatgcccgtt cacattagcc agttgggcca ttttctccac
     1681 ctccaattct cttgcaagtt tttctcgtcc actcatcctt gccgcatcgt atctctggag
     1741 cgctcttact gtggcaaggt ttcggtaaat cgaagcaaat ccagcgtaaa gggataagcc
     1801 aattatcatg aaatagcctg cagtcttgtt gccggccagg atccctatgg tcggcaagac
     1861 acccgcagcg aagaaaacgg ccgccaacag tcccacagcg aacgtgtacg ctgggttatc
     1921 aagtatcagt atcactgccc tacgtatcga tttccaaaca gtcgtgtcct gttccactat
     1981 tatggggtag gcgtacatga acagaagtcc aacaaaaatg ctgagatata cccagacccc
     2041 cgacaagaat ctcatcaaag aactgtccgt cccaagaagc acctgtatgt ctatccatag
     2101 aatcagaatg acagccgagt gtatcgctcc aagcaaagcc gcccttgtcc aaaacttctt
     2161 gtaaccctgc cacagttctt tcaatcctgc gtcttcgcga tgcgccgcca ggtttccaca
     2221 gtacattatc gccatgtgaa gtggagcagc aacgacagca aagattggaa tcgcccatat
     2281 tgtggggcca gaaataaaaa cagtggtaaa tgtggcaaag taccatatca cgctcatgag
     2341 cactaccatg ccgaggcgct cgtaggcaag acgaaaactc tttttcagca cgttccaggc
     2401 ttctacaggc gagtatctct ccatcacgct acatcctctc cggagcagat actcccatga
     2461 tccccagcac gtttcggagc acgattcttg cagcatcaac cagcgcaagc cttgcccggg
     2521 tctgggcttc atcatcactg atcactctgc agtcagtgta gaagctgtga aacaaccctg
     2581 ccagctccaa ggcgtacttt gcaatccggc tgggcgcccg ttcaagcgcg gcagtgagta
     2641 cttcatctgg aaagctcgcc atcttgcgga cgaggtcgat ctcgcttggg tgaacaagac
     2701 acgacacatc tgcctgctcc cactccgtag gctggattcc ctcggcctct gcctgtctga
     2761 agatgctgca gcagcgcgca tgagcgtact gaatgtacca caccggattg tccgtagttt
     2821 gggccttcgc aaggtcgagg tcgaaatcga gatgcgaaga ggtgtttctc attatgaaga
     2881 aataacgggc ggcgtccttc ggcacctctt ccagcaggtc atccatggtg ataaactcgc
     2941 ccgcgcgctt ggacatcttt acaggttgcc cgtctcgcat caacgtaact agttgaagaa
     3001 tgagtacttc caaggtgcca tctggatgtc caaaggcctt caaccccgcc tgcattctac
     3061 cttcgtaccc gtggtggtca ggaccgagta tgtctatgag cttcgtaaag ccccgcgcca
     3121 gcttgtctct gtgatatgcg agatccggcg cgacataggt atataggccg tcgcttttga
     3181 ccagtacccg atccttgtcg tcgccataat cagatgatct gaaccagact gcgccatctt
     3241 tctcataggt gtatcccgct tccgccatct cgcgaaccat atcctcaaca gcgccagtct
     3301 gccttagctt gcgctccgaa aaccatctgt cgaaacggac ctcgtaggcc tcgagcgacc
     3361 tcctctgcca atccaggatg tagttgagcg cgaactcttg tagaaaatgg cgagcgtcat
     3421 ccgaatccat cgcggagatt cgatcaccat gttcctccat agccttccta gcaacatcct
     3481 tgacgtactc cccgcgatac ccatcctccg ggaagggata atccactccc tcaagctcca
     3541 tgtaacgcgc atataccgac tccccaagca ccgacacctg acggcccgca tcattcacat
     3601 agaactcgct tgccgcgctg taaccacttg cgttaaggag cctgaccagg gtatcaccta
     3661 ccgcggcagc cctcgcgttg acgatgttaa gcggccccac cgggtttgcg cttacaaatt
     3721 ccaccaaaat gcgttcgcct cgtccatagt cgctgcaacc atacttctca tccctcgacc
     3781 tcaccaggcc gattgtatcc acaagccact tggatgaaag gtggaaattg ataaagcccg
     3841 gccccgcaat ctccgccctc tcaacatatg agctgaaccc ttcttgctcc atctcttcta
     3901 cgatcagcga tgcaatttcc cgtggcgctt tcctggcaac gcccgcaagg gacagagcca
     3961 tcggagtagc gaactccccg tgtctggcat ctttgggaac ctccagcgca atgctggaca
     4021 ggtggtccag tacatccgcg caaaggcgat tggaatcgac cagtttacgg atggcccctt
     4081 caagagatcg catgatgctt cctcgtatgt gttccattat gttgtccatt tgtacgtcct
     4141 ctccctgcct tctccgacca gtattcctcg aagcgccgcc agaatccttc ctagctcatg
     4201 ctggccatct gacgcggctc tgccccaaat ttcaacgagc tca
//
LOCUS       DAOSYL010000104         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_17163_length_4203_cov_7.704195, whole genome shotgun sequence.
ACCESSION   DAOSYL010000104 DAOSYL010000000
VERSION     DAOSYL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17163_length_4203_cov_7.704195"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..542)
                     /locus_tag="PLU15_10535"
     CDS             complement(138..542)
                     /locus_tag="PLU15_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOO31380.1"
                     /translation="MEKEGMVNAKEYGRRSKPRRAKSRPLAIRVNFEKAAFILAAMVL
                     ATVGLTLAFSQRPYVAPPLENLETVTVTVHPGDTLWGIASRFSDGNVDIRHVVRTISE
                     LNGLEGSIIRPGQGILVPVERPQVAGMRRPWR"
     gene            691..1308
                     /gene="lexA"
                     /locus_tag="PLU15_10540"
     CDS             691..1308
                     /gene="lexA"
                     /locus_tag="PLU15_10540"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004890619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOO31381.1"
                     /translation="MAMLTAREEQILQLISDQIEERGYPPSVREIGQAVGLKSTSTVH
                     GYLSSLERKGYIRRDAAKPRAIEVIMGVDRMPLPARDVLNVPLVGRVTAGQPILAVEN
                     IADYFPLPRSLAGECDSFLLQVSGESMVGAGILDGDYVLVRKQPTAENGDIVVALIED
                     EATVKRFYRDGNHVRLEPENPAFSAIITDKAMLLGKVIGLVRKIH"
     gene            complement(1404..1763)
                     /locus_tag="PLU15_10545"
     CDS             complement(1404..1763)
                     /locus_tag="PLU15_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31382.1"
                     /translation="MIHLAREDVLRGLERFRSIAKQDFLASGLTENPEFWAAQAAARR
                     AQYDQLIEIIETSGVPRAIEKAIECYNELPNLYDEPEFGQPETRGRKQALEVFFKAVG
                     VDRKTIRDARGQKTARM"
     gene            complement(1961..2362)
                     /locus_tag="PLU15_10550"
     CDS             complement(1961..2362)
                     /locus_tag="PLU15_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOO31383.1"
                     /translation="MRAGAPKRESDRAQIPIWKLVALGCGVCFLATLVLSAVGGILTT
                     LFTFDPTTINNAVPVIGYLSGGIGGLWAGKRGGKNGGRNGLLIGVVYLSIVLLISAAV
                     VREPMSLASVFTRGMSTIIVSTLGGIIGVNL"
     gene            complement(2436..2993)
                     /locus_tag="PLU15_10555"
     CDS             complement(2436..2993)
                     /locus_tag="PLU15_10555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO31384.1"
                     /translation="MPDLDAVAREAKECRRCKLGGMRKNAVPGEGNPHARIMFVGEGP
                     GADEDAQGRPFVGAAGRVLDKLIASMGLKREDVFIGNVVKCRPPGNRVPAPDEVEACM
                     PYLARQIELIDPDILVILGATALKALVDPNASITRMRGKWLERSGIRIMPTFHPAALL
                     RDTSKKALVWEDMKKVRACMKDFEA"
     gene            3389..3465
                     /locus_tag="PLU15_10560"
     tRNA            3389..3465
                     /locus_tag="PLU15_10560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3423..3425,aa:Pro,seq:cgg)
     gene            3495..3578
                     /locus_tag="PLU15_10565"
     tRNA            3495..3578
                     /locus_tag="PLU15_10565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3528..3530,aa:Leu,seq:tag)
     gene            3724..4191
                     /gene="mscL"
                     /locus_tag="PLU15_10570"
     CDS             3724..4191
                     /gene="mscL"
                     /locus_tag="PLU15_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOO31385.1"
                     /translation="MLKEFQEFAVKGNVIDMAVGIIIGGAFGKIVSSLVNDIIMPPIG
                     LLVGKVDFSNLFIDLSGNGYATLAQANEAGAPTINYGLFINNVIDFLVVAFSLFLIVR
                     WINTLRATAAAAIEEEAPCASAPSTKECPYCLSEIPVKAIRCAHCTSDLSDGR"
BASE COUNT          927 a         1224 c         1036 g         1016 t
ORIGIN      
        1 cacatgtggc attgatcacc gttttgtccg caattgcctc gcacatctgt gatgaaatcc
       61 tgataatctc aggcagttcc ggcactcaca accccccact catgtcccaa catggggtcg
      121 cgcttccggc gggccgctca gcgccatggc cgtctcatgc cggccacctg tggccgctca
      181 acaggaacca ggataccctg cccgggccgg attattgagc cttccaatcc gttcagctcc
      241 gagattgtgc gcaccacgtg cctaatgtca acattgccgt ctgagaaccg cgacgcaatg
      301 ccccatagcg tgtcccctgg gtgcacagtc acggtgaccg tttctagatt ttctagcggc
      361 ggagccacgt atggcctctg agagaacgcc aatgtgaggc ctactgtggc aagcaccatc
      421 gcagctagga taaatgcagc tttttcgaaa ttgacgcgga tggcgagcgg cctcgatttg
      481 gccctgcggg gtttggatcg tctgccatat tcctttgcat tcaccattcc ctctttttcc
      541 acagatgctt cccccttggc aaacagatgt tctggatcca atccaaggtt aacagaacat
      601 atgttctgtg tcaagggctg acccgaacga atgtttgcaa cagctcggct gctgtgatat
      661 actttcctca gctgaagggg gttgatgggc ttggctatgc ttaccgccag agaagaacag
      721 atcctccaac tcatatccga tcaaatcgaa gagcgaggtt atccgccatc ggtcagagag
      781 attggtcaag ctgtagggtt gaaatcgacc tctactgtcc acggatatct ctcatccctc
      841 gaacgtaagg gatacatacg ccgcgatgcg gccaagcctc gagcaataga agtgatcatg
      901 ggcgtcgacc gcatgcccct gcccgctcgc gatgtcttga acgttccctt ggtggggcgg
      961 gtcactgccg ggcaacctat tctcgctgtt gaaaacattg cggactactt ccctctcccc
     1021 cgctctttag ctggggagtg cgattccttc ctgcttcagg tgtccggcga aagcatggtg
     1081 ggtgcaggca tactcgatgg cgactatgtt cttgtcagaa aacagcccac cgcggaaaat
     1141 ggcgatatcg tagtggcttt aattgaggat gaggcaacag tcaaacgctt ctatcgcgac
     1201 ggtaatcatg tgaggctcga gccggagaac ccagccttct ctgccataat caccgacaaa
     1261 gccatgcttt tgggaaaggt tatcgggctg gtaaggaaga ttcactgatt tttgtggacc
     1321 acagcgaagc cgggctggat tcatcccgcc cggcttctat atgtttacag aggaaatgcc
     1381 ctgtataaaa aagcaaaacg cgcctacatg cgcgccgtct tttgcccccg agcatccctt
     1441 attgttttgc ggtctacgcc aaccgctttg aagaagacct caagcgcctg ttttctacca
     1501 cgcgtctccg gctgaccgaa ttcgggctca tcataaaggt tcggcagttc gttgtagcat
     1561 tctatcgcct tctctatggc ccgcggaact ccagatgttt caataatctc tatcagctga
     1621 tcgtactgtg cccgtcgggc tgctgcttga gccgcccaaa actccggatt ttccgtgagg
     1681 ccgcttgcga ggaaatcttg cttcgcgatg ctcctgaaac gctcaagccc ccgcagaaca
     1741 tcctcacgcg cgaggtgaat catctatcat tcccccttgt cggattttgt gggactaaag
     1801 attcactcca gttggtttaa tcgttcgcca ttggtcggca gattcctcct tgtcctgctc
     1861 acgaccgatt gtaccaaaag aataggcaac cagcatacgc cggctgccca ggcagacgtt
     1921 ctcgaatctt gtcgacacgc attttgttgt agctaccacc ttataagttg acgccgatga
     1981 tccctccgag agttgaaacg attatcgtgg acatcccacg tgtgaaaacc gatgccaagc
     2041 tcatgggttc cctgacgacg gctgcagata tcagaagaac gatgctaagg tagaccacac
     2101 cgatcaagag tccgtttctc cccccattct tgcctcccct cttgcctgcc cagagaccac
     2161 ctattccccc gctaagatat cctatgacgg gaacagcgtt attgatagta gtagggtcga
     2221 aggtaaacag ggtcgtgagt attccaccga cggcggacag taccagtgtg gcaaggaaac
     2281 aaacgccgca accgagcgca accagcttcc aaatggggat ctgggcgcgg tcgctctcac
     2341 gtttcggagc acccgccctc ataatctccg cacctcctca aggtcattgc cctcatcgga
     2401 agagtattag tctgcgctcg cagttattct tccccctacg cttcaaagtc tttcatgcag
     2461 gccctgacct tcttcatgtc ttcccagacc aaggctttct tgctggtatc gcgaagcaaa
     2521 gccgccggat gaaatgtggg cattatccgg atcccgcttc gctcaagcca tttgcctcgc
     2581 attcgcgtga tggaagcgtt cgggtcaacc aaagctttga gcgccgtggc gcccaagatt
     2641 acgagtatgt ccggatctat cagctcgatc tgacgcgcca ggtagggcat gcaagcctcc
     2701 acttcatccg gtgctggcac acgattaccc ggaggacggc atttcacgac attccctatg
     2761 aaaacgtctt cgcgtttcaa gcccatcgat gcaatgagct tgtccaaaac ccttcccgca
     2821 gctcccacaa acggcctccc ttgcgcatcc tcgtcggcgc caggtccctc accaacgaac
     2881 attattcgcg catgcggatt cccttctcca ggaactgcat tcttccgcat accacccagc
     2941 ttacagcgtc tacactcttt tgcctcgcgc gctactgcat caagatccgg caggtcgggg
     3001 gccctgacaa cactcgtttc ccacagcccc tttggatgag cggataaagg tacagccaca
     3061 ggagcgctta tgtgagcagc tgcggaccag gacggtattg cctgcgcagt cgactgattc
     3121 gactcggctt cggcgctcca taggttcatc tgttccacca tggaatcttc cgcatcacca
     3181 tgttcgaaga gcgtgagttg cttctcctct gccatgacat ctccctcttt ctatcctgta
     3241 ctaattatgg cacatgttgc gcaggtcttc catgcttgcc cttgcgctga gcactcagca
     3301 ttccggatca acaaattccg gcgcgatttg gtttgacact gctgcggcaa tgccatatac
     3361 tattgatgtg ctctgagggg cgcaaggtcg aggtgtagcg cagtttggta gcgcgcttgg
     3421 ttcgggacca agaggccccg ggttcaaatc ccggcacctc gaccattctt atgcctcggc
     3481 tcgcagcagt acgtgcgaga gtggcggaac ggcagacgca tcagacttag gatctgacgg
     3541 gtaaaaccgt gagggttcaa atccctcctc tcgcaccatg ttcaacacaa actcccctag
     3601 actgcatcag ccttaagatg gcctattgca gttttttcat ttacagcagg catatgattg
     3661 gccaaaccga gttttccaat tgtgacggga acgccgcttt atcggtgaag gagtgattaa
     3721 ctaatgctaa aagagttcca agaattcgcg gtaaagggca atgtaatcga catggctgta
     3781 ggaataatca tcggaggcgc cttcggaaaa atcgtatcgt ctctggtaaa tgacataatc
     3841 atgccgccca taggccttct tgtaggaaag gtcgatttct ctaacctctt cattgacttg
     3901 tctggcaacg gatacgccac tttggcgcag gcgaacgagg ccggcgcccc caccatcaac
     3961 tacgggctgt tcataaacaa cgtcatcgat ttccttgtag tcgccttttc gctctttcta
     4021 atcgtgagat ggattaacac tctccgggca acagccgctg cggccattga agaagaagcg
     4081 ccatgtgcat ctgcgccttc cacaaaagag tgcccatact gtctgtcgga gataccagtc
     4141 aaagctattc ggtgcgccca ctgcacttca gacctttctg atgggcgata atcgtggccg
     4201 gtc
//
LOCUS       DAOSYL010000105         4116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_17621_length_4116_cov_7.990396, whole genome shotgun sequence.
ACCESSION   DAOSYL010000105 DAOSYL010000000
VERSION     DAOSYL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17621_length_4116_cov_7.990396"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLU15_10575"
     CDS             <1..223
                     /locus_tag="PLU15_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOO31386.1"
                     /translation="MSIDKIQAEALKLHPSERARLAEALLLSLEDLSDEENERLWAEE
                     AQRRHEDLVAGRASERTAADVFRDIRQRL"
     regulatory      681..754
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            808..1525
                     /locus_tag="PLU15_10580"
                     /pseudo
     CDS             808..1525
                     /locus_tag="PLU15_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1803..2624
                     /locus_tag="PLU15_10585"
     CDS             1803..2624
                     /locus_tag="PLU15_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO31387.1"
                     /translation="MFPNERVCEVHCNVAAKPRTLRIGICEDSIPDMELIRTYLSGWK
                     ARVNSGDRPQAVIPAGIELRIFEFPDAEAFYFNYPDNPRFDVLLLDIHMGDMDGFELA
                     KRIRQKDRRIAVVFITGDPDYASKGYDVDAAGYLIKPVRREELYAVMDKCILSLKRGR
                     MELLWETDGELRKIYQEDILYFESAGHMITAVTADASFESRMSWAELESLISPDMFAR
                     THRSYIIGLGHVDVLGKTEVTLDNGIVLPVSRRLERELREKFVGYYSGGDGVWFP"
     gene            2612..3391
                     /locus_tag="PLU15_10590"
     CDS             2612..3391
                     /locus_tag="PLU15_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOO31388.1"
                     /translation="MVPVISLSYAHLVFIVAALIAFFWWFYKVALTRTVDRRIRNYQK
                     DLLERHFEEVNEVYGKMRGFKHDFHNHVQVMEACLELGKYDEMAAYLSQLSEDLEEID
                     WIIRTGNLMLDSILNVKLTLAEKRGINVNARASVPEALTDKGGTIITDVELCTVIGNL
                     MDNAIEGCLTLDDSEARFIRLLIRPMKRNLYVYVSNSYGGELRRKGDAYATTKEGHDH
                     GFGIRSVDGIVAKYGGSVNRQSEPGVFATEILLPLGDSSPS"
BASE COUNT          985 a         1004 c         1162 g          965 t
ORIGIN      
        1 catgagcatc gacaagatcc aagctgaggc tcttaagctc catccaagtg aacgggccag
       61 gctagctgag gcattattac taagcctgga ggatctctcg gatgaggaga atgagcgtct
      121 gtgggctgag gaagctcagc gccggcacga agacctcgtt gctgggaggg ctagcgagcg
      181 cactgctgca gacgtgtttc gcgatatccg ccagcgacta tgattattcg gcatgtcacc
      241 ttccatgaac tggccgaaca ggagctactt gatgcggcac actactatga aaatcagagc
      301 gcaggcttgg gatttgcatt cctcgaagaa atagaacatg cggttgcgca gatttgtgag
      361 catcctgaat cgacccacca aatatccccg cttgttcggc gcaagattgt gcgccggttc
      421 ccctatagta tcatgtaatc agttcaggac gataggattc ggatcctcgc tatagctaat
      481 caaagacggc gtcctttcta ttggcgtggg cgtcggtagg catgagaatc cctaacacac
      541 gagtttgccc agtcacctaa actgcgcccc taaagggttt gatgtggcgt gttggcagga
      601 atcgaacctg cggcctcgaa ccaggagtag cctattcgat aagagtgtcc attgacgcta
      661 cgctattgat agctgatata gtagtatcga gggatgaagc tcccctctgg agtaatccga
      721 accgctgacg acaactgatg gcttctacaa tcacacgtag gaaccgtcgg cttttgtttt
      781 tgggtatcgg ttccaggggg gattttgatg tctataggcc ttgctgctgc aacaattggt
      841 ggattgcttg gatacgctct gttcagtaga atcagaatgc ctggtctgct tgggatgcta
      901 cttgcaggca tcgtgcttgg gccgtatggg ttgaacttga tttaccacga attgattgca
      961 gtgtcggcag atctgctctt ctgatgagtc tgttgccagc ttcgtttgag cttctaggtg
     1021 taataattct tgcgccgaaa ctgctaggag taagcacttt ggaagcagcg attggtttgg
     1081 gtttggtatg gtttttcggg cgagtgcaca tccgcgacac agtcaaggcc ttgacaatca
     1141 tatccttggc catagtcatg gtttggctgg agagcttgct ggcgccagta tgcctatgtc
     1201 cggcttgctc gccgtcatgg caatgggtgt aatgctcctg gagcgcgatg aggccctggc
     1261 agagcgcttg agggtcagat ttgagaaact gtgggttgag ttgatcggtt cgaagctcaa
     1321 cttccacgaa aagctattct ccatgatggc atatactccc aaatccacag tgcaagccgc
     1381 cataggcgcg gtgcccctgg ccgtcggcgt cgaaagcggc gagctaatct tggccgtggc
     1441 tgtgctgtca atattgacaa ctgccccact aggcgcaact gccatcaaac tgaccgcgac
     1501 gcgtttgcta acaactgatg gctagaccga gctcttgggt gagttcatat cgacacccca
     1561 ggacatccgg gagaggcaaa tcgttgacac agtatgggcc acaggcagct gctatggtgc
     1621 gtcgcttgta gtttgcgcgg aagaatccag aacttgcagg gtcgacgcgg gttgtgtatt
     1681 acttccataa tccggtcgct agattcatcg catggttgtc caagggccat ggaaccgcag
     1741 gagcgatcat ggcagggaaa gactgtaaca gcgacgaaaa cacaaggcgc aggaagaaac
     1801 ctatgtttcc gaacgagcgc gtctgcgagg tgcattgtaa cgtggcggcg aagccgagaa
     1861 cactgagaat aggcatatgc gaagactcaa ttcccgacat ggaacttatt aggacgtatc
     1921 tttccgggtg gaaggctaga gtcaacagcg gcgaccgccc gcaagcggtc attcccgctg
     1981 gcattgaact tcgcatcttt gaatttcccg acgccgaagc gttttatttc aattaccccg
     2041 ataacccgcg cttcgatgtt ctgctgttgg acatccacat gggagacatg gacggcttcg
     2101 agctggccaa aagaatacgc caaaaagacc gcaggattgc cgtggttttc atcaccggcg
     2161 accctgatta cgccagcaag ggttatgatg tggacgccgc agggtatctc ataaagcctg
     2221 tgcgccggga agagctttac gccgtcatgg acaagtgcat tctgtcgctc aagaggggca
     2281 gaatggaact actatgggag acagacggcg agctgcgcaa gatctatcag gaagacatcc
     2341 tctattttga gtctgctggg cacatgataa ctgcggttac ggccgatgcg agtttcgagt
     2401 ccaggatgag ctgggcggag ctggaatctt tgatctcgcc cgacatgttc gcgcgcacgc
     2461 acaggtcgta tatcataggg ttgggtcacg ttgatgtgct cgggaaaacc gaggttactc
     2521 tggataacgg gattgttttg cctgtgagcc gcaggcttga acgagagctg cgggagaaat
     2581 tcgtggggta ctacagcggg ggcgatgggg tgtggttccc gtgatatctt tgagctatgc
     2641 acaccttgta ttcatcgttg ccgccctaat cgcatttttc tggtggttct acaaggtggc
     2701 tctgactcgc actgtggatc gccgcatacg caattaccag aaggacctgc tggagcggca
     2761 cttcgaggaa gtgaacgagg tatacggcaa aatgcgcggg ttcaagcacg attttcacaa
     2821 tcacgtgcag gttatggaag catgcctcga actggggaaa tatgacgaga tggctgccta
     2881 cctctctcag ctttccgaag atcttgaaga gatcgattgg ataatcagga ctggcaatct
     2941 catgctggat tccatactca atgtgaagct taccctggca gagaaaaggg gcataaacgt
     3001 gaatgccagg gcttctgtgc ccgaggcttt gaccgacaag ggcgggacga ttattacaga
     3061 cgtggagctt tgcactgtga taggcaacct catggataac gccatagaag gctgtcttac
     3121 tctggatgat tcggaagcgc gcttcatacg cctcttgata cgcccgatga agcgcaacct
     3181 ttacgtatac gtgagcaact cctacggcgg ggagctccgc cgcaaaggtg acgcctacgc
     3241 taccacaaaa gaaggccacg accacgggtt cggcatacga agcgtcgacg gcatagttgc
     3301 caaatacggc ggttcggtca accgccagag cgaacccggt gtgttcgcca ccgaaatact
     3361 gttgccattg ggcgacagta gcccatcgta atctactcta tgttgctcgg ctgccgtttg
     3421 aggcccggac aggaagtgtg cggcggggga ggagggagtg ttaacgaact tggccaactt
     3481 ccagtcgcat cgcgattgca agtgttcaag ttttagacat atgccattga ggcgaatcag
     3541 gggatacttt ttgtatggat atgcccaatg cataaaccag ccctcactcg caaaaagagc
     3601 tcaacgtgtg tcaataggcg gttaccgagc tttccccgaa aaacggtaca tgcaggcaaa
     3661 tgaaagatca agctgtgctg attgcttggc ctgcatggag caaccatata agccgcctat
     3721 cgggcaactt ttccaaagct gcttagacag ttgatggcca catccccctg cctcatgtaa
     3781 accacgccct ctccgttgcc gttcgtgacg aatatgatac cgctcatgtc acaaacgatt
     3841 tgactcgcat tttccatgat atattgcttt tggcgtgaat agatcattca gcggcaggag
     3901 gcgcgtacca tggaagaaaa cgaggcaaaa ggggtgctgg ccgaacaagc gccaaaacgg
     3961 tattcgccat ggcagaacat gggctagctg ctcaacgaca tagcccgtac cgataaatgg
     4021 tatctcacgt tcatgatact gatggtacct gtacagatag ccgtcatagc gtttgaagcc
     4081 tacataccca aggcggttat aggcggtatc ggtcaa
//
LOCUS       DAOSYL010000106         3760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_19666_length_3760_cov_5.460189, whole genome shotgun sequence.
ACCESSION   DAOSYL010000106 DAOSYL010000000
VERSION     DAOSYL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19666_length_3760_cov_5.460189"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1428)
                     /locus_tag="PLU15_10595"
     CDS             complement(46..1428)
                     /locus_tag="PLU15_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOO31389.1"
                     /translation="MRPRDEYAEPYRVKMVEPIKMTTREERIGKIEGAGYNVFNLRAE
                     DVYIDLLTDSGTPAMSDTQWAALMCGDEAYAGSKSFYRFRDVVRNITGYEYVIPAHQG
                     RGAEHVIMTALVKDGDAVLGNMHFDTTMGHILLRGAEPVNLLKKEGYEVESTEPFKGN
                     IDIEALERELDKRGSEKVPFVLMTVTCNSNGGQPVSMENIKAASEIAHKHNVPLFFDC
                     ARFAENSWFIKNREPGYENKSLLEIAQEMFSYGDGCIMSAKKDALVNIGGFAAVRDPD
                     IYHKAIEWEIPFEGYLTYGGLAGRDLEAMAQGLSEVLDDDYLEDRIGQVAYLADLLLE
                     RGVPVITPAGGHGVYVDAKAVLPHIPQSEFPAQAMTVELYIEGGVRGVELGACAFART
                     DPATGETIYPELELVRLAIPRRVYTDRHMLQVARAFEGILERRSSIRGLRITYEAPVL
                     RHFTAEFERV"
     gene            complement(1654..3237)
                     /locus_tag="PLU15_10600"
     CDS             complement(1654..3237)
                     /locus_tag="PLU15_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31390.1"
                     /translation="MEEPRYSAKSRVLAAVCISVAIVLSFFLLSGEARAHEGQWKYGG
                     SSAFKVYWQNALRIDNSTSLYIFGTTGPLRVGVQAKVLAANWGIPGEALMLGPLGEWT
                     ERWSVSLQAPMASLQLGDVYIPTMSGLYLAGRSLHGGVGSVWGSAGGVSGTVTGFCGT
                     NAVSSGFTVNSYQVSGGSAEASFGRKIGLSLRGLSGERDGFDVDLVGARIWAALGNAD
                     ITGELVGSRNNPVGESGYTALAGARVPACGGVLSLSGKYASDEFVSLNPAVSGSKGGN
                     AETSISWSGDVLRSSAGHAMRFAVTGTLAADNMDGSAPVQNVERSAESQVTLVSGRGQ
                     LIEGKYTVLHWESDEEPAPNYIKTKHVASIESAFPVKMGPGTVDVGTRAIHSVVIDHV
                     GRFRDALDLLSMSGAGSIGETEYAVKAGWSRSVRDTAGYEKRDLEASLSLSRPIAAGL
                     FIGGLNATAYDSRAFGAGDAEPESVKDALKVRVWLRYASKPWMEAAIGANATRAWKGP
                     EMAPHDLDCFIQGELKVRF"
     gene            complement(3519..>3760)
                     /locus_tag="PLU15_10605"
     CDS             complement(3519..>3760)
                     /locus_tag="PLU15_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31391.1"
                     /translation="TSQALSAGVFAEKIGVTGLGLHGGLVFRQRLDASGGGLVANVGA
                     RMALSDPLFLGVDASMGLTGGVAGTEVGFTVGYSF"
BASE COUNT          856 a         1198 c          873 g          833 t
ORIGIN      
        1 acccaagcga gctaagacaa cccggctttt tcagaccaag cccatctaca ctctttcgaa
       61 ttccgcagtg aagtggcgca gcaccggagc ctcatacgta attcgcagtc cccggatgga
      121 gctccgcctc tcaaggatgc cctcgaaggc ccttgccact tggagcatgt gcctgtcagt
      181 gtagactcgt cgcggaatag ccagcctgac cagttccagc tctggataga tagtctcgcc
      241 agtggccgga tcagtccggg cgaaggcgca tgcgccgagc tccacgccac gaaccccgcc
      301 ctcgatgtac agttccaccg tcatcgcctg ggccggaaac tcagattgcg gaatatgggg
      361 caacacggct ttggcatcaa catatacgcc atgtccgcct gcgggcgtta tcaccggcac
      421 tcccctctcc agtaaaaggt cggcgaggta ggcgacttga ccgatgcggt cctcaaggta
      481 gtcgtcgtcc aacacttccg aaagaccctg cgccatggcc tcgaggtctc gtccggcaag
      541 gccgccgtat gtcaaatagc cttcgaaggg tatttcccat tcgatggcct tatgatagat
      601 gtcggggtca cgcacggcgg caaaccctcc gatattgacc agcgcatctt tctttgcgct
      661 cattatacag ccgtctccgt aggagaacat ctcctgggcg atctcgagca aactcttatt
      721 ctcatatccg ggttcgcgat tcttgataaa ccacgagttt tcggcaaacc tggcacaatc
      781 aaaaaatagc ggcacattgt gtttgtgcgc tatctcactg gccgccttta tgttctccat
      841 ggatacgggt tgcccgccat tgctattgca cgttaccgtc atgagcacga acggaacctt
      901 ctcggatccg cgcttatcaa gctccctctc gagggcctca atatcaatgt tgcctttgaa
      961 cggttcggtg ctttcgacct catacccctc cttcttcaag agattgaccg gttctgcgcc
     1021 tcggaggaga atgtgaccca tggtggtgtc aaagtgcata tttccgagga ccgcatcccc
     1081 atctttcaca agtgccgtca tgatcacgtg ttcagcgccc cgcccctggt gagccgggat
     1141 cacgtattcg tagcccgtga tgttccgcac tacgtcccgg aagcggtaga aactcttgct
     1201 gccagcgtag gcttcatccc cacacatgag ggcagcccat tgagtgtcgc tcattgctgg
     1261 cgttccgctg tcggtgagaa ggtcaatgta tacatcttcg gctctgaggt tgaagacgtt
     1321 gtacccagca ccctcgattt tcccgattct ttcctccctc gtggtcatct tgataggttc
     1381 aaccatcttg actcgatacg gttctgcata ctcgtcacgc ggtcgcatgg caaatcacct
     1441 cacagttagg agatattcgc cattgtatac tcccatcctt caaaaacccg tttccctttt
     1501 ggagaagccc ggagattacg ccaagtctag gctgccccat tgcctgtcac acctacaaat
     1561 gtcatggata tgttgaggca agtcaaacgg cagaagaaga gtttgccgaa atcgagagtg
     1621 cgcgtgcgtc ctcacagcca caaatgggca gccctaaaac ctgaccttca gctctccctg
     1681 aatgaaacag tctaaatcat ggggggccat ctccggcccc ttccacgccc gcgttgcatt
     1741 tgcccctatc gccgcctcca tccaaggctt ggacgcgtac cgtagccaaa ctctaacctt
     1801 taaagcgtcc ttaacactct ccggttcagc gtcccccgcc ccgaatgcgc gcgaatcgta
     1861 tgctgttgca ttcaagcccc caatgaataa tcctgccgcg atggggcggg acaacgaaag
     1921 tgaagcctcg aggtcacgtt tttcgtaacc cgcagtatcc ctgactgatc tgctccatcc
     1981 ggccttgaca gcatattccg tctctcctat gctcccagcg cctgacattg acagaagatc
     2041 aagcgcgtca cgaaagcgac caacgtggtc tatgacaacg gaatggattg ccctagtgcc
     2101 aacatcgaca gtgcccggcc ccatctttac aggaaaagcc gactcaatag acgcaacgtg
     2161 ctttgttttt atgtagttcg gcgccggttc ctcatctgat tcccagtgca atactgtgta
     2221 tttcccttcg atgagctggc cccgcccgga aacgagggtc acctgagatt cagccgagcg
     2281 ttccacattc tgaacgggcg ccgagccatc catgttgtcg gcagctaacg tcccggtgac
     2341 tgcaaaacgc atcgcatgcc ctgctgagct tcgcagcaca tcgcctgacc acgaaatcga
     2401 cgtttccgca ttgccccctt tgctacctga aaccgccgga ttaagcgaga caaactcatc
     2461 gctggcatat ttgcccgaca gagacaacac tccgccgcaa gcaggcactc tggcgcctgc
     2521 aagcgcagta tagcccgact cacctaccgg gttgttcctg gatccgacaa gctctcctgt
     2581 gatatcggca ttccctagag cagcccagat tcgcgctccg accagatcaa catcgaagcc
     2641 gtcccgttcg cccgaaagac cgcgcaacga caggccaatc tttctaccaa agctggcttc
     2701 tgcagagcct ccacttactt ggtatgagtt aacagtaaag cccgagctca cggcattcgt
     2761 tccgcagaat cctgttacgg tgccagaaac gcctcccgca ctgccccata cacttccgac
     2821 ccctccatgc aaggacctgc ccgccaagta aagccctgac atggtgggaa tatagacatc
     2881 tcccagctgt agggaggcca ttggcgcttg caatgaaacg ctccaccttt ccgtccattc
     2941 acccaaaggt cccaacatca gcgcctcacc tggaattccc caattagccg ccaacacctt
     3001 ggcctgcact cctaccctca atggacccgt ggtgccaaag atatacagac ttgtggagtt
     3061 atcgatcctg agcgcattct gccaatagac cttaaacgcc gaacttcctc cgtatttcca
     3121 ttgtccctcg tgcgctctag cttcacccga caatagaaaa aaagacagca caatggcgac
     3181 agaaatgcaa acagccgcta aaacacggga ttttgcggag tatcggggtt cttccatgag
     3241 caagcccccc acccttcact tgacgtcgac aactgtatca agcagttgcc gacatcgccg
     3301 tcatttgatg gtattactta cgtcatggtc ccatattttc ctgcatctgc taacaaatca
     3361 cccgggtgac ccccattgca tcccctgacc caacaagaag cagggggctt cgacatgttc
     3421 gaatccccct gcaatcatcg gtgctgtcag gctctgcctc cagtgggcaa cagctgacaa
     3481 acgaccattc tatttgagaa caagcgcgag gcgcttccct aaaaagaata ccccaccgtg
     3541 aatccgacct cagtccctgc cactccgcca gtaaggccca tagaagcgtc aactcccagg
     3601 aaaagcgggt ctgagagagc catccgcgct cctacattgg cgacgagtcc gccacctgag
     3661 gcatctaacc tttgcctgaa cacgagaccc ccatggagtc caagacctgt caccccgatc
     3721 ttctcagcaa aaacccccgc cgaaagagcc tgtgaggtgt
//
LOCUS       DAOSYL010000107         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_19870_length_3727_cov_6.929739, whole genome shotgun sequence.
ACCESSION   DAOSYL010000107 DAOSYL010000000
VERSION     DAOSYL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19870_length_3727_cov_6.929739"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1936)
                     /locus_tag="PLU15_10610"
     CDS             complement(68..1936)
                     /locus_tag="PLU15_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31392.1"
                     /translation="MIAEANVEKNERGGVAGVRQGIRLLGRLDLALARTSVILGIFEA
                     AVPLVAVYMGKRVIETLSSGTGEVMRVVLWAIFLNLGLTLVSKWLQVQFSYGQAEMWR
                     KKDIAVNNKLMEVDYEVVEDPRIHEKLAWLQQTEKMLDRGVPAQADYLKNMARGTFTL
                     VAGLVMSLGVFSVPLPGTDMPLFLQNTLFLALIIALQLYSLWAGARNARGINEISESV
                     TLPMNMLFSAYWPHWQEYSYGKDTRLYTGKALNNALRQMPAVFDRIYDAFMGKYTSIG
                     VRTVANDILTMGLVYGFVGVKAYYGLITPPDIVLYAGTVTLMMSGLTNLAQNYSKAVR
                     NIPYLTVLGDFLDIESERYQGTLPVEKRDDNDYVFEVKNVSFKYPGSDKYALKDVNLK
                     LSVGEKLAVVGMNGSGKTTLIKLLTRFYDPTEGEITLNGVDIRKFNYDEYLELFSVVF
                     QDLKLFSFDIAQNVAAGISPDEERVTEALHMAGFDERLAEMPEGIHTYLYKNFAESGV
                     EISGGEAQKIAMARALYKDAPFVILDEPTAALDPISEFEIYTNFDNMVRGRTAVYISH
                     RLSSCRFCDDIAVFDEGRMVQRGSHDELLRDTGGKYYELWNAQAQYYKQEEIDALL"
     gene            complement(1933..>3727)
                     /locus_tag="PLU15_10615"
     CDS             complement(1933..>3727)
                     /locus_tag="PLU15_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31393.1"
                     /translation="VIAFEAYIPKAVIGGIGQVTTLQAYLWSIFYPVLALLAAKCLSV
                     ILDSQLVKGGANQRALYMSDAYTKSMDMDYQFLASDQGQAMVDKGLTLLDQGDMTLIV
                     KFGQRCSGLWAGLGGIIFFAGLVVRIHPCLLIFIIVSGILQYYYGKHVANFKEKMVGE
                     TRGLRAKMRYMRFRAGNYEYAKDMRMYKMADWFSDVDKDLEDEWSSYRREEKKRDYGG
                     FVLTAFMIFLRDGLTYFYLATMFLAGSIGVADFVFLLGITRGISGWLMDIIAEVTAIH
                     KDMAPLNDMREFFEYKDALNRDEGVPVPCRHDIQFKDVWFKYPGSEDQAIKGMNFHIK
                     DGEKLAIVGVNGAGKSTIVGLIVGFLHPDKGEILIGGTNAEKMNIYDRYALFSAVFQD
                     VHVFPDTVGNIVMGNDYEALDFMQNTGLGAASDSRMVSAETDARRKRVEEALEKAGLM
                     ETVKALPHGIDTYLNKMSRQGAVDLSGGQNQRLMLARAIYRDAPITVLDEPTAALDPI
                     AESEIYESYSQMVRGKTAVFISHRLASTRFCDRILFIEDGQIAEEGSHEELMGLGGKY
                     KEMFDIQAYYYQDNLDDEGGVAASAAGGETA"
BASE COUNT          859 a         1086 c          860 g          922 t
ORIGIN      
        1 gatgagctct tcaaggcatt caggtcaata cctattgcag gcaaatagca ccacagagca
       61 ggcaagccta cagcaaagcg tctatctcct cctgtttgta gtattgggcc tgggcgttcc
      121 aaagctcgta gtacttgccg ccggtatccc gcagaagctc gtcgtgactg cccctctgca
      181 ccatacggcc ctcgtcaaac accgcgatat cgtcgcagaa cctgcacgac gaaagcctgt
      241 gagagatgta gaccgcagtc ctgccccgca ccatgttgtc gaagttggta tatatctcga
      301 attcggatat agggtcaagc gccgcggtgg gttcgtccag tattacgaaa ggcgcatctt
      361 tgtataaagc gcgcgccatg gctatcttct gcgcctcgcc tccggagatt tccacgccgc
      421 tttcggcaaa attcttgtaa aggtaagtgt gtatcccttc gggcatctcg gccaatctct
      481 catcgaaccc cgccatatgc aaagcctctg tcacccgctc ttcgtcaggt gatattccgg
      541 cggcaacgtt ctgagctatg tcgaaagaaa acagcttcaa atcctggaac accacggaaa
      601 acagctctag atattcatcg tagttgaact ttcggatatc taccccgttg agcgtgattt
      661 ccccttccgt tgggtcgtaa aacctggtga gcagctttat tagcgtggtc ttgccgctgc
      721 cgttcatgcc caccacggcc agcttttcgc ccacactgag cttgaggttc acgtccttga
      781 gcgcatactt gtcagaacca ggatacttga agctcacgtt cttgacttca aacacgtaat
      841 cgttatcatc ccgcttctct acaggaagcg tgccttgata ccgctcgctt tcgatgtcca
      901 gaaaatcgcc aagaaccgtc aggtacggta tgttcctcac agctttgctg taattctggg
      961 caagattcgt aagccctgac atcatcaaag tcaccgtgcc ggcgtagagc acgatgtccg
     1021 gcggagtaat gaggccgtaa tacgccttga cgccgacgaa gccatagacc aaccccatag
     1081 tgagtatgtc gtttgccacg gttcttacac caatgcttgt gtacttaccc ataaaagcgt
     1141 cgtatatccg atcaaacacc gcaggcatct gccgaagagc gttgttcagc gctttcccag
     1201 tgtagagcct ggtgtctttg ccataggaat attcctgcca gtgcggccag taagcgctga
     1261 acagcatatt catgggaagc gtcaccgact ctgatatctc atttatcccc cgcgcattgc
     1321 gcgccccagc ccacagagag tagagctgca aagcgattat caaagcaaga aacaaggtgt
     1381 tctgcaagaa gaggggcatg tcggtacccg gcaggggaac agagaaaact ccaagggaca
     1441 tgacgagtcc tgccaccagg gtaaaagtcc ctcttgccat gtttttcagg taatcggcct
     1501 gggcgggcac cccgcgatcc aacattttct ctgtttgctg cagccatgcc agcttttcgt
     1561 ggatgcgcgg atcctccacc acttcgtagt cgacttccat cagcttgttg ttgacggcaa
     1621 tatccttctt gcgccacatc tccgcctgac cgtagctgaa ctgcacctgc agccatttgg
     1681 atacgagggt aagcccgaga ttgagaaaga tggcccacaa cacgaccctc atgacttcgc
     1741 ccgtgcctga ggaaagcgtt tctatgaccc ttttccccat gtacaccgcc acaagaggaa
     1801 ccgccgcctc gaaaatcccc aggatcacgg aagttctggc cagcgctaga tcgagcctcc
     1861 ccaaaagcct aatgccttgt cgcactccgg ccacaccgcc ccgttcgttt ttctccacat
     1921 ttgcctctgc gatcatgctg tttcacctcc cgctgccgat gcggcgactc cgccttcgtc
     1981 atcgaggtta tcctgataat aataggcctg gatgtcgaac atctctttgt acttgccgcc
     2041 aagtcccatc aactcttcat gactgccttc ttctgctatc tgtccgtcct ctatgaacag
     2101 aattctgtcg cagaacctgg tggatgccag acggtgggat atgaacacgg ccgtcttgcc
     2161 acgcaccatc tggctgtaac tttcgtatat ttcgctttca gctattggat ctaatgcggc
     2221 ggtaggttcg tccaacaccg ttatgggggc atcacggtaa atggccctgg ccagcatgag
     2281 tctttgattc tgtccgcctg acaggtccac agcgccctgc ctggacatct tgttgagata
     2341 cgtgtcgatg ccgtgaggca gcgccttcac cgtttccatc agccccgcct tttccagagc
     2401 ctcctcaacc ctcttacgcc tagcatcagt ttctgcggaa accatcctgg agtcggacgc
     2461 agctcctaac cccgtgttct gcatgaaatc caatgcttcg taatcattgc ccatcacgat
     2521 gttgcccacc gtatccggaa acacgtgcac atcctgaaac accgccgaaa acagggcgta
     2581 ccggtcataa atgttcattt tctcagcgtt ggtcccgccg atgagtattt cacctttatc
     2641 tgggtgaagg aacccaacga tgagaccgac tatagtgctc ttccccgcgc cgttgactcc
     2701 cactatggcc agcttttcgc cgtcttttat gtgaaaattc atgcctttta tcgcctgatc
     2761 ctcggaaccc ggatatttga accacacatc tttgaactgt atatcgtgcc ggcaaggcac
     2821 gggaacgccc tcgtctcggt taagcgcgtc cttgtactcg aaaaactcgc gcatatcgtt
     2881 taggggagcc atgtctttgt gaatggccgt gacctcggcg attatgtcca tgagccaccc
     2941 tgaaattcct cgggttatac caagaagaaa cacgaagtct gcaaccccaa tactgcctgc
     3001 caggaacatg gttgccagat aaaagtaggt gagaccgtcc cttaagaaga tcatgaaggc
     3061 tgtgagaacg aatccgccgt aatcccgttt tttttcttct cttctgtatg aactccattc
     3121 atcttctagg tccttgtcca cgtcagaaaa ccagtccgcc atcttataca tgcgcatgtc
     3181 cttggcatat tcgtaattgc ctgcgcgaaa acgcatgtac ctcatcttgg ccctgagccc
     3241 ccgggtttca cccaccattt tttctttgaa gttcgccacg tgtttcccgt aataatactg
     3301 gaggataccg gaaactatta tgaaaatcaa aaggcaggga tgtatcctca ctaccaggcc
     3361 cgcaaaaaag attatgccgc ccagtccagc ccagagaccg gaacaacgct gcccgaactt
     3421 gacgataagg gtcatgtccc cctggtccag aagcgtcaga cctttatcta ccatggcctg
     3481 cccttggtca gatgctaaga actgataatc catatccatg cttttagtgt aagcgtcgct
     3541 catatagagg gctcgttgat tagcgccgcc ctttactaat tgagaatcca gaatcacgga
     3601 caaacatttg gcagccagaa gtgcaagcac ggggtagaat atgctccaaa ggtatgcctg
     3661 cagtgtcgtc acttgaccga taccgcctat aaccgccttg ggtatgtagg cttcaaacgc
     3721 tatgacg
//
LOCUS       DAOSYL010000108         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_20071_length_3698_cov_7.244579, whole genome shotgun sequence.
ACCESSION   DAOSYL010000108 DAOSYL010000000
VERSION     DAOSYL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20071_length_3698_cov_7.244579"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLU15_10620"
     CDS             complement(<1..265)
                     /locus_tag="PLU15_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31394.1"
                     /translation="MRSSRWSEMCITRYAPLIQITWTGPYCWPQFETEGNSRPLPKQP
                     GVYLQTVEYQDGYLIYAAGLTRRSIPIRFREHTRKYMSGDYTVL"
     gene            434..871
                     /locus_tag="PLU15_10625"
     CDS             434..871
                     /locus_tag="PLU15_10625"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOO31395.1"
                     /translation="MSIGKIQAEALKLHPSERARLAEALLLSLEELSDEENERLWAEK
                     ARRRHEDLVAGRASERIAADVFRDARGRLWAMIVERVIFHELAEQELLDIANYYENQS
                     AGLGFDLGLGEKNSLRVEKETICTMCLLDKACTAWTLSPDARI"
     gene            complement(1092..1382)
                     /locus_tag="PLU15_10630"
     CDS             complement(1092..1382)
                     /locus_tag="PLU15_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOO31396.1"
                     /translation="MIRLNCKQVLHLHQQMIAVTGGTSGVRDQGALDSAINHAFASVE
                     GKDLYPTVEEKAARQCFSIVQNHPFVDGNKRTGLFAMLVFLDMNGVKLHFKQ"
     gene            complement(1379..1621)
                     /locus_tag="PLU15_10635"
     CDS             complement(1379..1621)
                     /locus_tag="PLU15_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOO31397.1"
                     /translation="MIINTKQMIPLADANRNFSKVARAADQQGAVVILKNNVPKYVVL
                     SFDHFEGLSKSEDERLESVAARILKENLPAFSELAK"
     gene            1969..2103
                     /locus_tag="PLU15_10640"
     CDS             1969..2103
                     /locus_tag="PLU15_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31398.1"
                     /translation="MMIRRACAVFAAAVILGRVLMPVMVAKAWATEPGHRGYVRMVTP
                     "
     gene            2587..3018
                     /locus_tag="PLU15_10645"
     CDS             2587..3018
                     /locus_tag="PLU15_10645"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOO31399.1"
                     /translation="MESSFAFLNSPAPKSSISITPTSRASMFCCWISIWETDGELCKI
                     YQEDIFHFESVGHMMTAGTADASVESRMSWAELESLISHDMFARTHRSYIIGLGHVDV
                     LGKTEVTLDSGTVLPVSRRLEKELREKFVGFHVGCDAVWFP"
BASE COUNT          835 a          888 c         1060 g          915 t
ORIGIN      
        1 ctaggactgt gtaatccccg ctcatgtact tgcgagtatg ttcacggaat cgtatcggaa
       61 tcgaacggcg cgtgagcccg gctgcgtaga tcagataacc atcctggtat tcaactgtct
      121 gcagatagac acctggctgt tttggaaggg gacgcgaatt accctcagtc tcgaattgcg
      181 gccagcaata tgggccggtc cacgtaatct ggattaaggg cgcataccgg gttatgcaca
      241 tctctgacca cctcgaactc ctcatcaaat tggctccatc ggcccacata atcttgattc
      301 gacgctgact tcctggtttc ctatgccaga tagcccgctg gaaacgcttc aacccagtca
      361 gtcagaatcg tgcagtttgt tgggccagct ggcgtcagat gacataatag aaagggaagg
      421 aggcgactct tccatgagca tcggcaagat ccaagctgag gctcttaagc tccatccaag
      481 tgaacgggcc aggttagctg aggcattact gctaagcctg gaggaactct cggatgagga
      541 gaatgagcgt ttgtgggctg agaaagcccg gcggcggcac gaagacctcg ttgctggaag
      601 ggctagcgag cgcattgctg cagacgtgtt tcgcgacgcc cgcgggcggc tatgggctat
      661 gattgttgag cgtgtcatat ttcatgagct ggccgaacag gagctacttg atattgcgaa
      721 ctattatgag aatcagagcg caggcttggg ctttgatctg gggctaggcg agaagaactc
      781 gcttcgggta gagaaggaaa ccatatgtac catgtgtttg ttggacaagg cgtgcacagc
      841 ttggacgctt tctcccgatg cacggatctg aagatactgt tgggagccat agttgcgcgc
      901 ctatatcgag cggttggctg ttcacttgaa gatgtggcaa gctgcagata ttgtgcacat
      961 cgcccggctc acgcttgttc gcgttgagag ccagacaggg ttcatgtgat cctgtgatct
     1021 atgatccacc tcaggatcct gtctgagttc atagtcccgt tggcaatgcc taggccaagc
     1081 tcaacgagtt cttattgttt gaagtgtagc ttcactccat tcatatccag aaaaaccagc
     1141 atggcgaaga gtccggttcg cttgttgcca tctacgaacg gatggttttg gactatcgaa
     1201 aagcattgac gagcagcctt ttcttctact gtggggtaca gatccttgcc ttcaactgat
     1261 gcaaaagcgt gattgatagc cgagtcgagt gcgccctggt cgcgcacgcc tgaggtcccc
     1321 cctgtgaccg caatcatctg ctgatgaagg tgaagcactt gcttgcagtt gagtcggatc
     1381 actttgccag ctccgaaaac gctgggagat tctccttcaa gatacgggca gccaccgatt
     1441 ccagccgctc gtcttcagat ttggataacc cctcgaagtg gtcaaaactg agcacgacgt
     1501 acttgggcac gttgttcttc agaatgacca ctgctccctg ctggtccgct gcacgtgcca
     1561 ctttggagaa gttcctgttg gcatcagcta aagggatcat ctgcttggtg tttattatca
     1621 tgtcgatcac ctcatcccca gtatactcaa tatgtaggca agaataaacc tacatttact
     1681 tactgctatt acgcagctgt ttcgttccgc ttcagcaaag tagcttgatt agcccttcca
     1741 cgattcctcg gtttccgctc caagccttcc gctaaggcca ccagggagga aatagatatt
     1801 tacacagtga atgtggaaat cgtggaggac gggtggatga ctccgagcga gatcaaagcg
     1861 cgggtacgtg tagcaacggg caagtagggc gcccaagctg attggctgga gttgcttctg
     1921 tgatatgaac gggtggcgtg ctgccgtatc aggaaggagg cgagctgtgt gatgattcgc
     1981 agggcctgtg ctgttttcgc tgcagctgtg attctagggc gtgtgttgat gccggtcatg
     2041 gtcgcgaagg catgggctac ggagcccggg caccgaggat acgtgcgcat ggtgactcct
     2101 tgatgatact tgctgttaac tgtgcctgtg ggtaccctgc tttcgtctgg accaggtgag
     2161 cgatttcacc aggatacgaa gttcgggagt cgctggcttc cacgggcgac gtttttctgc
     2221 aatcggggcg ccttctctgc gttctgcggg agaatccaaa attggcaggg gcgacgcggg
     2281 ttatgtgttg cctccataac cggccgctgg attcattgca tggttgtcca agggccatgg
     2341 agctaaagga gcaatcatag cagagaaagg ctgtaacagc gacgaagaca tcagccccag
     2401 aaagaaaacg ctattcccga ttcagcgcat ctacgaggtg tattgtgacg tggcggcaga
     2461 acgcagaaca ctgagaatag gcatatgcga agactcactt ctcgatatgg aacttattga
     2521 ggggtacctt tttgggatgg gaggccggag tcaatagcgg agacagttcg caagcggcca
     2581 ttcctgatgg agtcgagctt cgcatttctg aattctccgg cgccgaagag ttctatttca
     2641 attaccccga caagccgcgc ttcgatgttt tgctgttgga tatccatatg ggagacagac
     2701 ggcgagctgt gcaagatcta tcaggaagac atcttccatt ttgagtctgt cgggcacatg
     2761 atgactgccg gtacggccga tgcgagtgtc gagagcagga tgagctgggc ggagctggaa
     2821 tctttgattt cgcacgacat gttcgcgcgc acacacaggt cgtatatcat agggctgggt
     2881 cacgttgatg tgctcgggaa aaccgaggtt accctggaca gcggaactgt tttgcctgtg
     2941 agtcgcaggc ttgaaaaaga gctgcgggag aaattcgtgg ggtttcatgt cgggtgcgat
     3001 gcggtgtggt tcccgtgata tctttgagct atgcgcatct tgcgctcatc atcgtcgccc
     3061 tgatcgcatt tttctggtgg ttctacaagg tggctctgac ccgcaccgtg gatcgccgca
     3121 tacgcaatta ccagaaggac cttctggagc gggatcctag catcaagcaa catagtattc
     3181 gcctgtgccg aagatggttc caaggcagcg tcggcgaacg gcaaagctag cagactgaag
     3241 attcaactcc gatacggcaa gatccgtgct cgtatgatct ttaagttcgc ttttcgcatg
     3301 tgggttttgc cataatatca tttctcctcg aatggattcg ggccgatggc tgcgtggagg
     3361 gtattatccc attcgcataa cacgcgccaa attgaatcgg cttggatcca tattgccgtc
     3421 cgaaatccta ttgttcttag ggcttgcatt ttacgtaggg attctgcatg gcccagaacg
     3481 ggcgttctta accggtcatt gataggcagc gacgaacagc gggttctata tatcaataag
     3541 ggaaggacgg atagtcgtga tgggctgtga tgcctacaac ctggcctgta ttgggccggc
     3601 tggcgtcaga tgatataata ggaaagaaag gaggcggctc ttccatgagc atcgacaaga
     3661 tccaagctga ggctcttaag ctccatccaa gtgaacgg
//
LOCUS       DAOSYL010000109         3683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_20167_length_3683_cov_6.183848, whole genome shotgun sequence.
ACCESSION   DAOSYL010000109 DAOSYL010000000
VERSION     DAOSYL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20167_length_3683_cov_6.183848"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="PLU15_10650"
     CDS             <1..1641
                     /locus_tag="PLU15_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO31400.1"
                     /translation="TGERLGRVLVNSGHVSEGLIATVIERQLGIPRVSLAEYVVADDV
                     IKIVPENMARRYKVLAIERDGNRLTLAMADPLNVLAIDDIKLATGFEVSPVVAPEDEI
                     VRALDQYYGSSEDLDEVVRYLEKYSEDAIRFASYEEEIGVDRLRELVDEAPVVRLVNM
                     IIAQAVGERASDIHVEPHATGVRVRYRVDGILREVMDLPRGSHAAIASRFKIMSNMDI
                     AERRVPQDGRVQLKVEGEEIDLRVSTLPTIYGEKVVCRVLFRRGAMVKLEQLGFLPDN
                     LELWHRALSCPYGIILVTGPTGSGKTQTLYASLNQLNSVEKNIITIEDPVEFRLDGIN
                     QVQTNPKAGLTFASGLRSMLRQDPDIIMVGEIRDRDTAEIAVNAALTGHLVLSTLHTN
                     DAPGATVRLVDMGAEPFLVASSLICVLSQRLVRKVCTHCREDYVPDDEEWGRWVRMTR
                     GLSDESGLGLPRRLVRGKGCRQCGNTGYRGRTAIHEIMMVTEPIRELVSGNASSDVVA
                     EVAREQGMRRLIEDGADKVRMGITTLDEVMRVAAGSEV"
     gene            1708..2787
                     /locus_tag="PLU15_10655"
     CDS             1708..2787
                     /locus_tag="PLU15_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOO31401.1"
                     /translation="MKIDGLLMEAVNLGASDLHLTTGLPPMVRVSGALKALEGHLPLK
                     PADTREMMYSIMKPDIRERYEAAGEVDFSHGMPGVARFRVNAYTQRGSVGAAFRVIPT
                     RIRSLEELGLPPVVKTFADKTRGMVLVTGPTGSGKSTTLASLIDIINSERACHVITLE
                     DPIEYLHKHRKSMINQREVGTDTKSFAAALRAALREDPDVILVGEMRDLETTSIALTA
                     AETGHLVFATLHTNDTVQTVDRIIDQYPPHQQQQVRVQLAAVLQGIVSQILLPRADGQ
                     GRIVAVEVLVANPAVRNLIREGKTHQIYSVLQTGAKQGMVSLDMYLVDLAKRGIITYD
                     DALSHATNPDEMRRLGQFAQPIMRL"
     gene            2806..>3683
                     /locus_tag="PLU15_10660"
     CDS             2806..>3683
                     /locus_tag="PLU15_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO31402.1"
                     /translation="MASLSWSYRGRDMSGQVVEGTMEAENPETVAERLRSEGYVPVMI
                     TEAKRSVFSADASAASGKKVQAATSLDTMQLSLISRQMSTMVAAGIPLVQTLAILSQQ
                     IEDPRIAELINEVRRSVESGETFAASLAKHPGCFPPLFVHMVEAGEASGTLDIVMNRV
                     ANYYEQQVKIRSQLKNALVYPIILVAVALVVVVILMAFVLPTFIDMFEGFDMELPWVT
                     RAVFGYGQSVQRAWYLHFGIPIVTIVWFVSYVRGAGKSWWDHTAIRLKISGPIIRKSQ
                     TAQFARTFAMLTQSGVP"
BASE COUNT          808 a          877 c         1197 g          801 t
ORIGIN      
        1 acaggagagc gcttgggcag ggtcctcgtg aactcggggc atgtgagcga gggtttgatt
       61 gcaactgtga tcgagcggca gctgggcatt cccagagtga gccttgcgga gtatgtggtt
      121 gctgatgacg tgatcaagat tgttcccgaa aacatggcaa gaaggtacaa ggttcttgcc
      181 atcgaaaggg acggcaacag actgaccctt gccatggctg atcctctcaa tgttctggcg
      241 attgacgaca taaagctggc taccgggttc gaagtcagtc ctgtagttgc gcctgaggac
      301 gaaatagtcc gcgcgttaga tcagtactac ggttcttctg aggacctgga tgaagtagta
      361 cgctacctgg agaaatactc tgaagatgcc atccggtttg ccagctacga agaggagata
      421 ggcgttgacc gtctgcgcga gctcgttgac gaagctcccg tggttaggct ggtcaacatg
      481 ataatcgccc aggctgtggg agaacgggcg tcagatattc atgtggagcc gcatgccaca
      541 ggggtcaggg tcagatacag agtagacgga attctccgcg aggtcatgga tcttcccagg
      601 ggaagccatg cggcgatcgc ctcgcggttc aagataatga gcaatatgga tatagctgaa
      661 aggcgcgtgc ctcaggacgg cagagttcag ctgaaggtgg agggcgaaga gatagacctc
      721 agggtgtcga cgctgcccac catctatgga gagaaggtcg tctgccgcgt gctgttcagg
      781 cgcggggcca tggtcaaact ggagcaactt gggtttctcc ccgacaacct ggagctttgg
      841 catcgggcgc tttcttgccc ctatggcatt attctggtca cgggccccac aggtagcggg
      901 aagacgcaga cgttgtatgc atcgctgaac cagttgaact cggttgagaa gaacataatc
      961 actatagagg atcctgtgga gttcaggctt gacgggataa atcaggtgca gacgaatccc
     1021 aaggcgggcc tgacttttgc ttcagggctc agatcgatgc tgagacagga ccccgacatc
     1081 ataatggtgg gagagatccg ggatcgcgat accgccgaga tcgcagtcaa tgcggctctc
     1141 acgggccacc tggtcctctc gacgctccac acaaatgacg cgccgggcgc aactgtgcgt
     1201 ttagtggata tgggggctga gccgtttttg gtcgcgtcat ctttgatatg cgtattatcg
     1261 caaaggcttg tccgcaaggt ttgcacgcac tgtagggaag actatgtccc cgacgacgag
     1321 gagtggggca ggtgggtcag gatgacgcgt gggctttcag atgaatccgg cttggggctc
     1381 cctcgccgcc ttgtgcgcgg gaaagggtgt cgccagtgcg gcaacacggg gtaccgggga
     1441 cggacggcaa tacatgagat catgatggtg acggagccca tacgggagct agtgtcgggc
     1501 aacgcttcca gtgacgttgt ggcggaagtc gccagggagc aggggatgag aaggctcatc
     1561 gaggatggtg cggacaaggt ccgaatgggg attactactt tggacgaggt tatgagggtg
     1621 gcggccgggt cggaagtcta ggcgattcca tctgtacata tggggctttt gcttttcggg
     1681 agagtgatat cataggtgac tgatagcttg aagatagacg gattgcttat ggaggcggta
     1741 aatctcgggg cctcagacct tcacctcact acagggcttc ctccaatggt gagggtgagc
     1801 ggagctttga aagccctgga agggcatctt ccactcaaac cggctgatac tcgcgaaatg
     1861 atgtacagca tcatgaagcc ggacattcgg gagaggtatg aggcggcggg agaggtcgac
     1921 ttctcccacg gtatgccggg ggttgccagg ttcagggtga acgcttatac gcagcgtggc
     1981 tccgtggggg cagcgttcag ggtcattcca acaaggatca ggtccttgga ggaacttggc
     2041 cttccgcctg tggtgaagac gtttgccgac aagaccaggg ggatggtgct ggttaccggt
     2101 cccacgggaa gcggaaagtc gacgaccctg gcctctctca ttgacataat caattcggag
     2161 cgggcgtgcc acgtgatcac cttggaggac cccatagagt accttcacaa gcaccgaaag
     2221 agcatgatca accagaggga agtggggacg gacaccaagt cttttgcggc ggcgctcagg
     2281 gctgccctca gggaggatcc tgatgttatc ctcgtggggg agatgcggga tctggaaacg
     2341 acttctatag ctcttaccgc tgccgaaact gggcacctgg tgtttgccac gttgcacacc
     2401 aacgatacgg ttcagactgt ggacaggatc atagaccagt atcctcctca ccagcaacaa
     2461 caggtcaggg ttcagctggc ggcggtgctg cagggtatag tatcccagat tctgttgccc
     2521 cgcgcagacg gacagggaag gattgtggcg gtggaagtcc tcgtggcaaa tcccgctgtt
     2581 cgcaacctta tacgcgaggg gaagactcat cagatttatt cggtgctcca aacaggcgca
     2641 aagcagggga tggtgagcct tgatatgtac ctcgtcgatt tggcgaaacg cgggatcatc
     2701 acgtacgatg atgcgctgag ccatgcgacg aaccctgatg agatgaggcg gctaggccag
     2761 tttgctcagc cgattatgag gctttaggca agggggtgag gggaagtggc tagtctttct
     2821 tggtcgtatc gcgggaggga catgagcggg caggtggttg aaggcaccat ggaggcggag
     2881 aatccggaaa cagttgccga acgccttcgg tcggagggct atgttcccgt gatgatcact
     2941 gaagctaaac gatcggtgtt ctcagctgac gcgtcggcgg cctcaggcaa gaaggtgcag
     3001 gctgcgacga gccttgatac gatgcagctt tcgcttattt cccgtcagat gtccaccatg
     3061 gtggctgcgg gaattcccct ggttcaaacg ttggccatcc tctcacaaca gatagaggat
     3121 ccccgtatcg ccgagcttat caacgaggtg aggcgcagcg tggagtcggg ggagactttt
     3181 gctgcatcct tagcgaaaca ccctgggtgc ttcccgccgc ttttcgtgca catggtggag
     3241 gcgggggaag cgtcgggaac cttggacata gtcatgaacc gcgtggccaa ctactatgag
     3301 caacaggtga agattagaag ccagcttaag aacgcgctgg tttatccgat aatacttgtg
     3361 gcagtagctt tggtggtcgt cgttattctg atggctttcg tgttgcccac tttcatcgac
     3421 atgttcgagg ggttcgatat ggagctgccg tgggttactc gagccgtatt cgggtatggg
     3481 cagagcgtgc aacgggcgtg gtacctgcat ttcggtatcc caatagtcac cattgtttgg
     3541 tttgtaagct atgtgagggg ggcggggaag agctggtggg accatactgc aatccggctg
     3601 aagatttcgg gccccattat ccggaagagc cagactgccc agtttgcaag gactttcgcc
     3661 atgttaaccc aaagtggcgt gcc
//
LOCUS       DAOSYL010000110         3614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_20645_length_3614_cov_7.552683, whole genome shotgun sequence.
ACCESSION   DAOSYL010000110 DAOSYL010000000
VERSION     DAOSYL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20645_length_3614_cov_7.552683"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..917
                     /locus_tag="PLU15_10665"
     CDS             36..917
                     /locus_tag="PLU15_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOO31403.1"
                     /translation="MDLIATPVEALKDRLELTARRFRRRMEGLDRKSVGIALGSGAAR
                     GMAHIGVLEVLFHEGIHIDVVVGCSMGAVVGGCYASGMEAEAIEEVAVSINKLDVARH
                     VDLVSPTSGGLMAGSKLSALLKELTGEKTFDELRFPFACVAADILTGDEVPIDDGYVY
                     EAMRASSSIPGVFQPVVHGKRVLVDGGILNPVPANYLRALGVDLAIAVDVMPVLRPSE
                     EQLGRVPSIPSTLINSFDIMGRLVAAPLTRLADVVIKPDVGEVFGAELWRAPELIEKG
                     REAAKAAIPAICKALKV"
     gene            984..1910
                     /locus_tag="PLU15_10670"
     CDS             984..1910
                     /locus_tag="PLU15_10670"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOO31404.1"
                     /translation="MEYDSINVDMSQAWAGGSFSEREAMGVMEEMRFWLRIMSDHAKL
                     IRNGFDLAEEELFRFADALAENLDGMLARVQSVNPPFSPGEVQSFIAESNAVVVPLRE
                     FKADLEVAIRECRVMTDRPAELIDHVRREAEFFLGIINGLVGGPIPTRCNLKIPAPPG
                     KESEPAALAPRSLISCMGQFAQSILLDGILFFTHINGEHAQVLSIYFRPEIQEELAET
                     TKGYGNRLLCLLDEMTDKIVQGSSFQEMKDVVTRIREATIPWLEHLRTLKTKIGSCSV
                     PTGQVNFPYSLLDHMEREGMYSIELLDMALNM"
     gene            2963..3529
                     /gene="lepB"
                     /locus_tag="PLU15_10675"
     CDS             2963..3529
                     /gene="lepB"
                     /locus_tag="PLU15_10675"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO31405.1"
                     /translation="MQAILLAIVLAMVIMTFIGRSFVVEGASMEPTLHNRERIIVEKV
                     SYRFREPARGEIIVLKNPWRPDQSGMAAAMEAMRELVDFSGSMRPYIKRIVAVAGDRV
                     QIKNGVLYLNGKPIDEPYILERPWSDYGPVIVPEDHVFVLGDNRNNSDDSRGSVGFLR
                     VNRIVGRACVRYYPFDRMGVLKAPPVYE"
BASE COUNT          843 a          865 c         1074 g          832 t
ORIGIN      
        1 tcgactctag aagctgtatc cggaggtgta gccaattgga cctgattgcc actcccgttg
       61 aggctctcaa ggataggctc gaactgactg ccaggcggtt tcgcaggaga atggaagggc
      121 tcgaccggaa gtctgtcggg attgcgctcg gaagtggagc agcgcgcggg atggcccata
      181 ttggtgtgct cgaggtcctt tttcacgaag gtattcacat agacgtcgtc gtgggatgta
      241 gcatgggcgc cgttgtgggt gggtgctatg cctctggcat ggaagctgaa gccatcgaag
      301 aggtggctgt ttccattaac aagctggacg tggctcgcca tgttgacctt gtttctccaa
      361 ctagtggagg actgatggct ggctcgaagt tgagcgcgct gttgaaagag ctcaccgggg
      421 agaaaacctt cgacgagctg agattccctt ttgcatgtgt tgctgcagac atactaaccg
      481 gtgatgaggt gccgatcgat gatggatacg tctatgaagc aatgagggct tcgtcctcta
      541 taccgggcgt atttcaacct gtcgtccatg ggaaacgtgt actggttgac ggcggaatac
      601 tcaatcctgt gcccgccaac tatcttcgcg cccttggggt cgacctcgct attgcagtgg
      661 acgtaatgcc cgtgttgagg ccatccgagg aacagcttgg ccgtgttccg tcaatacctt
      721 caaccctaat aaactccttt gacatcatgg gaaggctcgt tgcagcgccc ctcacacgcc
      781 ttgcggatgt agttatcaag cccgacgtgg gtgaggtctt cggcgctgaa ttatggaggg
      841 ccccggagct cattgagaaa gggcgggagg ctgcaaaggc agccatcccg gcaatatgca
      901 aggctttgaa agtctgaact gtcatgaacc tgccacttgc cgactatcgt attgtgataa
      961 ggcaagccgg aggtgaatgt agcgtggaat atgattccat caacgtcgat atgtctcagg
     1021 cgtgggctgg aggatcgttt tcggagcggg aagccatggg tgtcatggag gagatgcggt
     1081 tctggctccg cataatgtcg gatcacgcca aactgattag aaacggattc gatctagcgg
     1141 aagaagagct attccgcttt gcggacgctt tggcagaaaa cctagatgga atgttagctc
     1201 gggttcagtc tgtgaatcct ccattttcgc ccggcgaagt tcaatccttc atagctgagt
     1261 cgaatgccgt tgttgtgccc ctcagggagt tcaaggccga cctggaagtg gcaatccggg
     1321 agtgccgagt gatgaccgat cgtccagcag agcttattga ccacgtaagg agagaagctg
     1381 aattcttcct cgggattata aacggtttgg ttggcggccc gattcccact aggtgcaatc
     1441 taaagattcc tgccccgccg gggaaggaga gcgagcctgc agcgcttgcg ccgcgcagcc
     1501 tcatatcatg catgggacaa tttgcacagt cgatcctcct cgacggtatt ctgttcttca
     1561 cgcacatcaa cggggaacat gcccaggttc tgtccattta cttcaggcct gaaatccaag
     1621 aggaactggc ggaaacgacc aagggttacg ggaacagact tctatgtctg ctagatgaga
     1681 tgacagacaa gatcgtgcag ggttcgagtt ttcaggagat gaaagatgtg gtgactcgga
     1741 tcagggaagc tacgataccc tggctcgagc atcttcggac tctcaagact aagatcggtt
     1801 cctgcagcgt gccaacgggt caggtcaact tcccgtattc tctgctcgat catatggaac
     1861 gtgaaggcat gtattcgatt gaattgctcg atatggctct gaacatgtaa ttctattgct
     1921 aatgctgata gaatccgcgc gtgggggccg acgaggcccc tcgcgcgttt actgggttgc
     1981 tcaccttatg gtaaccaaga ggaacatcgc ggcgcacagg atttgtctgg cggtctacag
     2041 ttgcgtagcc gtattgggcc tagtcgtctg cattatgcac gtgccttggt aggtcggcag
     2101 gaatgggtca gcagttgccg acaccgctag ggtgttgatg gtctttccga ctcaactgcg
     2161 caaagcggag caagccggct ctgttgcagt cgcatgtgga taggcccgcg caaggcgggc
     2221 ccaacgagcg atttgcccta gcacgggcgc atggtcccta ttacggcttt atggcccagg
     2281 cgcatcgaat aggcaatggg agatgcaaca gaccaagaag caaacgaaga agacgtgtag
     2341 ccaaaaccac catccgaaga agaaatgttc gccctttaca ggatctggca ttgcgcgctc
     2401 acctccatga taacctatgg catcttatga tctaacgcga aatcgggaat tgtgaacaaa
     2461 gcactgattt tcactggaga cactgcgcct atcaggcggt gatgcctccg agactccgcg
     2521 gcacttgcta tgtggtgcag taaacgatat tatcgtgtca gaagcccgca ggttgcggga
     2581 acatagtcgg cgtcgttttc gttataacta tgtagaactt acaatgacgc cgatgcagaa
     2641 ataaggtagg cgcaggttta tggataatgg catcgagaat gatggcgcag tcggcaaagg
     2701 cgagtcggca gtcgcttccg cgcacgacgc cgtatcgagc ggcccatgtt cgggtgagcg
     2761 tcctgattta tgtccggatg agggacgacc tgccgatgaa cgaattgaga atgtagacat
     2821 tagaagcgaa tcgaacgacg tagacatcga aggaatgatg gttggagcta tcgctgatga
     2881 agcagcggca gcagcggagg cagcgcgtct aagggaagca gccagcaagc gagaggctgc
     2941 gtttcgcgag gtaagagagt acgtgcaagc aatactcctt gcaatagtct tggctatggt
     3001 tattatgaca tttatcgggc gttccttcgt tgtggaagga gcttcaatgg agccaacgct
     3061 ccacaaccgt gaacgcataa tcgtggagaa ggtctcttat agattccgcg aacctgcgag
     3121 aggcgaaatc atcgtcttga agaatccttg gaggcctgac cagtccggaa tggccgctgc
     3181 catggaagcc atgcgggagc tcgtcgattt cagcggatcc atgaggccgt acatcaagag
     3241 aatcgtggcc gttgcaggcg acagggttca gatcaaaaat ggcgttctgt atcttaatgg
     3301 caaacccatt gacgagccgt atatacttga gcgcccgtgg tcggattatg ggcccgttat
     3361 agtccctgaa gatcacgttt ttgtcctcgg cgacaaccgc aataactccg atgacagtag
     3421 gggaagcgtg ggatttctcc gggtcaaccg tatagtcggc agagcctgcg ttcgctacta
     3481 tccgtttgac aggatgggtg tattgaaggc gccgccggtg tatgagtaat aatgttaggt
     3541 gcatgacacc gtagtccgag ataagcttga ggcatgcgaa tgacaataat gcccctgagc
     3601 gcacgctccg gggc
//
LOCUS       DAOSYL010000111         3608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_20678_length_3608_cov_7.184351, whole genome shotgun sequence.
ACCESSION   DAOSYL010000111 DAOSYL010000000
VERSION     DAOSYL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20678_length_3608_cov_7.184351"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2986
                     /locus_tag="PLU15_10680"
     CDS             <1..2986
                     /locus_tag="PLU15_10680"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOO31406.1"
                     /translation="EIVHRIGGAPIVSAPKPRSGEIYLGPDNSKANVFLWTTPYMEQD
                     KAYLVQNTGKIADAESPQNAHRYADGEASYTYCLPLDGLKLPAWLGLSSVGGQYKVSV
                     SNDDKNYEIIAVERRPVRDESNRTKIVYTITAHDVASGSLYVRFEDSNTEDGWGASFR
                     GGPLVIYSSADVADGYSYFPVLERPERLHVIRESAMDAAEFLTIISIQGIVAQDRPEI
                     YIQGAGNYGAWVDMFERDYGIPVEYHNDPMWFIEHYRNLFETNGYILTALETEAVNVA
                     TTAAGVLRAPIVDESLKPLMDSLGFEMVLDTTDLDEAWAFETYKEYVTEDLVVRQEWG
                     KIHLRDYAIATKAFTFYDLEDIFFMKDIYDWVRRDIPLIGWGPGSEGSHIGVATMMGL
                     FTVPADWALNLTVFPAVSVAKLSQPNHVANIRFETDVHYVTFVMSDGDNVQWFLNDMP
                     TSKRHYGSDRRGEFPMGFTISPSLADLAPTVMKWYYDTAKLDYYVAGISGTGYMYPVS
                     FPELDSYVDKCNEYLERSDLSIVSLHDPTPMNTPEFLEMAEAYARQPNVLGGIYLTGR
                     RYAGEEGNVVWADGKPFVGVRESLWTDDPVEMAGRINSYAADPSVPEGYTIVNVHPWS
                     HSLDDVATVIDNLAPHVRVVNPEEFFLQMIYNLGGRFPTVRTDVEAKISSPVSREKVS
                     GIIDVCGNASSSSAIAMVLATIDDMPWIIAEGADKWNVSLDTRKVADGPHALRVIAFD
                     EDGKNCQVSEEIMVMNQSDSGTIVGWVMYAHSPVAGAAVYLGERTCVTDQDGRFEFKF
                     VPSGMATLKVQAEGFTGISVDVAVAANQASVVELAFPVDPDTRRVWTFDEDADGWKGG
                     YISPGAPHMGKAKWDKNIGDGPGSLCMDGSDYGYPDGKANAWFYNIVKLPVDAENISY
                     ETRGNEKAGGIGGGLRLVLVDEEGEIHELTPWEASNAAEWVVRTADISEFAGRTVVLR
                     FEQDDLDVGISEHRWVDNVIIR"
     gene            3030..>3608
                     /locus_tag="PLU15_10685"
     CDS             3030..>3608
                     /locus_tag="PLU15_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31407.1"
                     /translation="MLVYRGTLTVAAIHDKRQGTMVKMAFWTTGDFHTHTFLTDGSHT
                     LFEVARRAFEIYGLDWIANSEHGGSSSRGPAGMYWENSLGPEEFEGILIKDEAGRRAM
                     WRWQSLRDYSFPLVERLRDTYPNNVIIQGVEWNMPCYDHVSVGIAADEPVAISDFEYI
                     FDQNDEDASRTHLNQFKRNSTREDAIAGVRWLN"
BASE COUNT          905 a          808 c         1061 g          834 t
ORIGIN      
        1 ggagatagtt cacaggattg gcggagcgcc tatcgtttca gccccgaagc cgcgctctgg
       61 cgagatatac ctcgggccgg acaacagtaa ggccaacgtt tttctgtgga ccactccata
      121 catggagcaa gacaaggctt atttggtcca aaacaccggg aagattgcgg atgccgagag
      181 tccgcagaat gctcacagat atgctgatgg cgaagctagc tatacatact gtcttccgtt
      241 ggacgggctc aaactcccag cctggctcgg actatcaagc gtcggtgggc aatacaaggt
      301 ttcggtatca aatgacgaca agaactacga gatcattgcc gtagagcgca gacctgtgcg
      361 ggatgagtcg aacagaacta agatagtgta cacgattaca gcacatgacg tggcctctgg
      421 atcgctatac gttaggttcg aggacagcaa cacggaagac ggctggggtg ccagctttcg
      481 aggcggaccg ctcgtaatat acagttcagc ggatgtagct gacgggtatt catatttccc
      541 agtattggag aggcctgagc gtcttcatgt aataagagaa tctgccatgg acgccgctga
      601 attcctgacg atcatatcta tccagggcat agttgcgcag gacagaccag aaatctatat
      661 acaaggggca ggcaactacg gtgcatgggt cgatatgttt gaaagggatt atggaattcc
      721 tgtggagtac cataacgacc caatgtggtt cattgagcat tacaggaatc tatttgagac
      781 gaacggctac atcctaactg ctttggagac tgaagctgtg aacgtggcaa cgaccgcagc
      841 tggagtactc agagctccaa tcgtggatga gtcgctcaaa cccctcatgg attctttggg
      901 gttcgagatg gtcctagata caactgactt ggatgaggca tgggcctttg agacatacaa
      961 ggaatatgta actgaggacc ttgtcgtgag gcaggaatgg gggaagattc acctcaggga
     1021 ttatgccata gctacgaagg ctttcacctt ctacgacctt gaagacatat tcttcatgaa
     1081 ggacatctat gattgggttc gtcgcgatat accgctgata ggatggggtc ctggaagcga
     1141 gggttcgcat ataggcgtag ctactatgat gggcctgttt acagtgcctg ccgattgggc
     1201 tttgaacctg actgtatttc ccgcggtttc agtggcgaag ctgtctcagc ccaaccacgt
     1261 ggccaacatc agatttgaaa ccgacgtgca ctacgtcaca ttcgtcatga gcgatggaga
     1321 caatgtgcaa tggttcctca acgacatgcc aacgagcaag aggcattatg ggagcgacag
     1381 gcgaggggaa tttcctatgg ggttcaccat ttcgccgagc ctagccgatc tggcgcctac
     1441 agtaatgaag tggtactacg acaccgccaa acttgattac tatgtagccg gcatttcggg
     1501 gactggttac atgtatccag tcagttttcc tgaactcgat tcatatgtcg acaaatgcaa
     1561 tgagtacctg gaacgatccg atctgtccat cgtgagcttg catgatccga ctccaatgaa
     1621 tactccggaa ttcctggaga tggcagaggc ttacgcgagg cagcctaacg ttctaggcgg
     1681 catttacctg actggtcgac ggtatgcagg cgaagaaggg aacgtggtgt gggctgatgg
     1741 caagccattc gtgggggtcc gcgaatctct gtggactgat gatccggttg agatggcagg
     1801 caggataaac tcttacgcag cagacccctc agttcctgag ggctatacta ttgtgaatgt
     1861 tcatccgtgg agccacagct tggatgatgt ggccacggta atcgacaatt tggcgccgca
     1921 tgttcgcgta gtcaatcccg aggagttctt tctgcagatg atatacaacc tgggagggag
     1981 attccctacc gtaaggacgg atgtggaggc caagatttcc tctcctgtaa gcagagaaaa
     2041 ggtatcggga ataatcgatg tttgcgggaa cgcatctagt tcgtctgcga tcgcgatggt
     2101 attggccaca attgacgaca tgccttggat tattgctgaa ggcgccgaca agtggaatgt
     2161 gtctttggat acgaggaagg ttgctgacgg tcctcatgcg ctgagagtta ttgctttcga
     2221 tgaggacggc aagaactgcc aagtatctga agagatcatg gtcatgaacc agtctgacag
     2281 cgggactatc gttggttggg taatgtacgc gcactcgcct gtggcaggtg ccgcagtcta
     2341 cttgggagag aggacatgtg ttaccgacca agacggcagg tttgagttca agttcgtccc
     2401 tagcgggatg gcaaccttaa aggtgcaggc ggagggattc accggcatca gcgttgatgt
     2461 tgctgtagcg gccaaccaag ccagcgtagt ggagctcgcg ttcccagtcg accccgatac
     2521 gcgtagggtc tggactttcg acgaagatgc tgatggttgg aagggcgggt acattagtcc
     2581 gggggcgccg catatgggaa aggccaagtg ggataagaac ataggcgatg ggccgggaag
     2641 cctttgcatg gacggctcgg attacggata ccccgacggc aaggcgaatg cctggttcta
     2701 caacatagtg aagcttcctg tcgatgctga gaacatcagc tatgagaccc gtggaaacga
     2761 aaaggcggga ggaatcggcg gcggactacg tcttgtgctc gtcgacgagg aaggcgagat
     2821 acacgagctg acgccgtggg aagcgagcaa tgccgcagaa tgggttgtca gaacggcaga
     2881 catatccgag tttgcaggtc gcacagttgt gctcagattt gaacaagacg atttggatgt
     2941 gggaatatcg gagcatcgct gggtcgacaa tgtgatcatc agataagaca acgaacttta
     3001 cttgtgaatg tgccgcggtg gcgcaggcaa tgcttgtcta ccgcggcaca ttgactgtgg
     3061 ctgcaattca cgacaaacga caggggacga tggtgaaaat ggcattttgg acaacaggtg
     3121 atttccacac tcacacattt ctcaccgatg ggagccatac gcttttcgaa gtggctcgtc
     3181 gtgcatttga gatatacggg ctagactgga ttgccaattc agagcatggg ggatcaagtt
     3241 cacgtggccc cgcaggcatg tactgggaga acagcctggg tcctgaggaa tttgagggaa
     3301 ttctcataaa ggacgaggca ggacgtcgtg ctatgtggag atggcaatct ctgcgcgact
     3361 actcctttcc tttggtggaa aggttgcggg atacgtatcc gaataacgtg atcattcagg
     3421 gcgttgaatg gaatatgcca tgttatgacc atgtaagtgt tggaattgcg gctgatgaac
     3481 ctgtcgccat atccgatttt gaatacattt tcgatcagaa tgatgaagat gccagtagga
     3541 cacatctcaa ccaattcaag cgcaacagca cgcgtgagga tgccatagct ggagttcgct
     3601 ggctcaat
//
LOCUS       DAOSYL010000112         3560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_21011_length_3560_cov_5.703281, whole genome shotgun sequence.
ACCESSION   DAOSYL010000112 DAOSYL010000000
VERSION     DAOSYL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21011_length_3560_cov_5.703281"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLU15_10690"
     CDS             <1..1001
                     /locus_tag="PLU15_10690"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO31408.1"
                     /translation="CQYKLILNGTPLSNNEQDLYAQWYFLDPRILGYHSYYSFAANHL
                     EFDPDYPQRVVAAHDVELLTRKIQPYTYQVRKHECLDLPPKTYSWRYCDMTLRQRAIY
                     EDAKDKILMDVDPDGWETHTIYRLFTALQKVVSGIQLDRTPIFAAPSDNPRVWTLMET
                     IEDLPADAKCIVWCKYTHEIEAVRQMLDPYGGVATIYGGVAPKIRDKELNRFREDARF
                     LVANKRVGAFGLNLQHCSYAIYYSNDFSWETRTQSEDRIHRAGQVNNCHIIDIVCSES
                     IDMRIQRCLARKERLVDSFRKEIDRTKDKTDWRRWIDGGEENVSESGCISGSEGAA"
     gene            943..2241
                     /locus_tag="PLU15_10695"
     CDS             943..2241
                     /locus_tag="PLU15_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HOO31409.1"
                     /translation="MARKTYLNQDVYRAAKARLEFIFDEFEHIYFSFSGGKDSGVMME
                     LALEVARERNRLPLHTLLIDLEGHYREHIEYSLRLCDRPAIKPYWICLPISLRNAVSQ
                     FEPKWVPWEDEKRDIWVRQIPDRPYVITEHNMPEEWTWFRKGQEFEEFILFFADWFAD
                     HYHADKTACVVGIRADESMNRYRTIVNDRKTRYADQGWTTLIKGLRNPVYNAYPIYDW
                     RVEDIWTYVGKAGCDYNKIYDYMYKTGRSVHDMRICQPYGDDQRKGLDLFQECEPETW
                     FKVVQRVSGANFGAIYRGGTILGNVKVELPPGHTWESYAKLLLSSMPPFLREHYIENF
                     NTFFRWWDQFGGGRTEHQKVVTDRDRYDEMGIYYSEHIPYDGYQGDDAPDTAKWRQPL
                     WKRICKVLLKNDYWCKELSFAQVKGEYDRLEALKEKYRDI"
     gene            2254..2859
                     /locus_tag="PLU15_10700"
     CDS             2254..2859
                     /locus_tag="PLU15_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31410.1"
                     /translation="MQIHDIDEHRLVLAELGQDDCSRVLDRRVHVVYNAPPNPDTINW
                     WRGYKQVSYTYQDFLNALFALYDQADAEEYYIECGKVHDAVVDYMSRRGFVIQERPIL
                     YSAPLHGHGAAMAKKRLPYTLVAASRKPVPVPVGEYSHEAIADALSRRQPGETVFDPC
                     IGKGLALRAAIARGMQCVGIEASEERLKCCIEYATKEAKSC"
     gene            2853..3485
                     /locus_tag="PLU15_10705"
     CDS             2853..3485
                     /locus_tag="PLU15_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOO31411.1"
                     /translation="MLSLFTSTLDEIKDAMRNHLSVMGTEERITALNEIRALLHELSP
                     QTEPVDYVRWVPADKVVANEYNPNKVAPPEMRLLHISIQEDGYTQPIVCFYDGDNDQY
                     VVVDGFHRTRVGTEKRDIRERIHGYLPVVVIDKPLDERMASTIRHNRARGKHAVSPMS
                     DLVATLYRGGWADDRIAKELGMELDEVLRLKQITGLPEIFADREYSRAWE"
BASE COUNT          868 a          907 c         1068 g          717 t
ORIGIN      
        1 ggtgccagta taagctgatc ttgaatggca ctcctctcag caacaacgag caggacttgt
       61 atgcccagtg gtatttcctc gacccgcgca tactgggcta ccacagctat tactcatttg
      121 cggcgaacca cttggagttc gacccggact acccgcagcg tgtagtcgcg gcgcatgacg
      181 tggagctgct aacacgcaag atacaaccct acacctatca ggtgcggaag catgagtgtc
      241 tggacctgcc gccgaaaacg tatagctggc gatactgcga catgactcta aggcagaggg
      301 ccatctacga ggacgccaag gataagattc tgatggatgt cgatcctgac ggctgggaaa
      361 cgcacaccat ctaccgactg tttacggctc tacaaaaagt ggtgtcgggc attcagcttg
      421 accggacccc gatatttgcg gctccatcgg ataatccgcg agtatggacg ctaatggaaa
      481 caatcgagga tctgcctgcc gacgccaagt gtattgtgtg gtgcaaatac actcacgaga
      541 tagaggcggt gcggcaaatg ctcgacccat acggcggggt ggccacgatc tacggaggag
      601 tcgcgcctaa gatcagggac aaggagctta acagattccg cgaggacgcg cggttcctgg
      661 tggctaataa gagagtgggg gcatttgggc ttaacctcca acactgctct tacgctatct
      721 actacagcaa cgacttctcg tgggaaactc ggacgcaatc cgaggatcgc atacaccgcg
      781 caggccaggt caacaactgc catattatag acatcgtttg tagcgagagt attgacatgc
      841 gtatacaacg atgcctagcg cgtaaggagc ggctagtaga ttcgttccgc aaggagatag
      901 accgcaccaa ggacaagacg gactggagga ggtggataga cggtggcgag gaaaacgtat
      961 ctgaatcagg atgtatatcg ggcagcgaag gcgcggcttg agttcatatt tgacgagttc
     1021 gagcatatct acttctcctt ctccggcggc aaagacagcg gtgtaatgat ggagttggcc
     1081 ctagaagtcg cccgtgaacg taatcgccta ccactccata ctctcctgat cgaccttgaa
     1141 gggcactatc gggagcatat cgagtattcg ctgcggctat gcgaccggcc cgcaatcaag
     1201 ccatactgga tttgcctacc catctccctg cgcaatgcgg tttcgcaatt tgagccaaag
     1261 tgggttccgt gggaagacga gaaacgcgat atttgggttc ggcaaatacc tgacagacca
     1321 tacgtgatta ctgagcacaa catgcccgag gagtggacgt ggtttcgcaa agggcaggag
     1381 tttgaggagt ttatactctt tttcgcggac tggtttgccg accactatca tgccgataaa
     1441 acagcctgcg tggtagggat tcgtgcagat gaaagcatga accgataccg caccatcgtc
     1501 aatgaccgca aaacccgata tgcagaccag ggctggacta cgctaatcaa gggcctgcgc
     1561 aatccagttt acaacgctta ccctatctat gactggcgcg tagaggacat atggacatac
     1621 gtgggcaaag ccggctgcga ctacaacaag atctatgact acatgtacaa gaccggcaga
     1681 tccgttcacg acatgcgcat ctgccagcca tacggtgacg accagcgcaa ggggctggac
     1741 ttgttccagg agtgcgagcc ggagacatgg ttcaaggtgg tgcagcgagt atcgggggca
     1801 aacttcggcg ccatctatcg tggcgggact atcttgggca atgtcaaggt ggagcttcct
     1861 ccgggccaca catgggagag ctacgccaag ttgctgctat ccagtatgcc gccgttcctc
     1921 agagagcact atatcgagaa tttcaacacc ttcttccgtt ggtgggatca gtttggcggc
     1981 ggacgtacgg agcaccagaa ggttgtcacg gatagggatc ggtatgacga gatgggcatc
     2041 tactattcgg agcatatccc ctacgacggc taccagggcg atgacgcgcc cgataccgcc
     2101 aagtggagac agccgctttg gaagcggatc tgcaaggtgt tgctcaagaa cgactactgg
     2161 tgcaaagaac tgtcattcgc gcaggtcaag ggcgaatatg accggctaga ggctttgaag
     2221 gagaaatacc gcgacatatg aggagggatg agcgtgcaaa tacatgatat cgacgaacac
     2281 agactggtgc tggcagaact ggggcaggat gattgttctc gcgtgctgga ccggcgcgtt
     2341 cacgttgtgt acaacgcccc gccgaatccc gacacaatca actggtggcg agggtataag
     2401 caggtttcct acacgtatca ggacttcctg aatgcgctgt ttgcgctcta cgaccaggcc
     2461 gacgcggagg agtattacat tgagtgcggc aaagtgcatg acgccgtagt ggactatatg
     2521 agcaggcgcg ggttcgtcat tcaggagcgc cctatcctct attccgcgcc gttgcatggg
     2581 cacggtgccg ccatggctaa gaagcggttg ccctacactc tcgtggcggc atccagaaag
     2641 ccggtgccgg tgccggtggg cgaatattcg catgaagcta ttgccgatgc tctctcgcgc
     2701 cgacagcccg gcgaaaccgt gtttgacccc tgcatcggca aggggttggc gctgcgggcc
     2761 gcgatagcga ggggcatgca gtgtgtcggg attgaggcaa gcgaggagcg cttgaagtgc
     2821 tgcattgaat atgcgacgaa ggaggccaag tcgtgctgag cctgtttaca tcgacactgg
     2881 atgaaatcaa ggatgcaatg cgcaatcatt tgagcgttat gggaacagag gagcgtataa
     2941 cggctctgaa tgagatacga gcactgctgc acgagctgtc gccgcagacg gagccggtgg
     3001 attacgtgcg ctgggtgccc gccgataagg tggtagccaa tgaatacaac ccaaacaagg
     3061 tcgcgccgcc ggagatgcga ctccttcaca tcagcatcca ggaggacggc tacacgcagc
     3121 caatcgtgtg tttctatgat ggggacaacg accagtatgt tgtagttgat gggttccacc
     3181 ggacgcgcgt cggtacggaa aagcgggata tcagggagcg catacacggt tatctgcctg
     3241 tggtggtgat agacaagccc cttgacgaga ggatggcgag cacgatacgt cacaacagag
     3301 cacgaggcaa gcatgccgta tcccccatgt ctgatctggt ggccactctc tatcgcggcg
     3361 gttgggcgga tgatcggata gccaaggagc tgggcatgga actggacgaa gtgctgagac
     3421 tcaagcagat cacaggcctc cccgagatat tcgcagaccg cgagtattcc agagcatggg
     3481 agtgataggc atgactgaat acaccgccat gagcgtcggc cagcgcctgg actatcgcaa
     3541 cgtcgatgcg gtgatgcggg
//
LOCUS       DAOSYL010000113         3532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_21199_length_3532_cov_11.479149, whole genome shotgun
            sequence.
ACCESSION   DAOSYL010000113 DAOSYL010000000
VERSION     DAOSYL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21199_length_3532_cov_11.479149"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..1621)
                     /locus_tag="PLU15_10710"
     CDS             complement(320..1621)
                     /locus_tag="PLU15_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31412.1"
                     /translation="MICLKISYRTRVSLGDQDGAALVTVLTVVVVLMVLAGAVSALAT
                     SSRIQALRQKNRVQAYFIARSGAEALVSYIVDNPQNLTIGEKMTFLEQLVDKGNSQET
                     AFATGSFTLSVSKMGAAEYAVTSVGKAGRVTQTVTYTIRALQPAPVDMAIFGYGPITI
                     ENNAEIDGDIITNSTAEGAVKIGKAKIMDCTIKTGPTETPNKVVSTSITPKPPIVSLD
                     SAREYPNPLPIAWPTEPDLAYGNLTIPKNAKWTIIAPAGTTKVITVDDLILKNKATLT
                     IDGTGTVLLYAKSIDFINNTEVNKDGEPDQLRIWCESLAALNNSVIKAHIHVDDSSVE
                     LKNNVTMEGNLVVRKEGATVDIDNNCKTQGIVYAPNCSVIVHPNVEVEGAIIAGTVTI
                     WNNAKVEGPNWDDDDCVTLTHTPLASGAVGGVADLKRGIWK"
     gene            complement(1672..2178)
                     /locus_tag="PLU15_10715"
     CDS             complement(1672..2178)
                     /locus_tag="PLU15_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO31413.1"
                     /translation="MHSCQRGATLVELLVAITLVGMVFAIAVTLFVFGQRTFALTLAQ
                     ADLQTSLTAASESIVSKVRNAVEVSVSDDASGKGADCIYLGYSGGVSTLLYDKEGASG
                     VPLVGTRVENVQFALASSAGGCVLTIDLASSSKGRSSKLSTSAYLANIKEGAFSPDSG
                     LYLRFTLP"
     gene            complement(2219..2584)
                     /locus_tag="PLU15_10720"
     CDS             complement(2219..2584)
                     /locus_tag="PLU15_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO31414.1"
                     /translation="MRVSDERGFTLMEVLVAIVVLSVSLGAILAAFNQIFGAVVMGER
                     LTKAVFAAEDAMSRSIPAKTPQGVAGSGTQPSVVGSDSSATLCLQFEGTGPIYVTGKQ
                     IDIGATFRSQEVPVSTWIP"
     gene            complement(2644..2916)
                     /locus_tag="PLU15_10725"
     CDS             complement(2644..2916)
                     /locus_tag="PLU15_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO31415.1"
                     /translation="MFLITHVGDDGFTLVEIVVVVVLLGLIGAFFYSGINAGMGTSWR
                     GKDKVKSVSVVQEVTEELTSSNLKPHVGRGDIACDEEWIPRRYSVR"
     gene            complement(3066..>3532)
                     /locus_tag="PLU15_10730"
     CDS             complement(3066..>3532)
                     /locus_tag="PLU15_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31416.1"
                     /translation="YRGYPAPEVASGDDSHIIVNGYRFFDGTLGKRDKHGEPSINDLT
                     IESPSGDGVLFVNGDLLAKNNLTFRGNITVIVVGDLVCKNSLKGENGKLHLVVGGDTR
                     DMNNMELAGTVTVGGSFKAKNNTTVTFRAVGDSGVPGYDPTEISDWSREWVR"
BASE COUNT          814 a          991 c          873 g          854 t
ORIGIN      
        1 ctaattgggt caaaaacccg tcattctgtg ccgccaaggg tgctgaagat gggttaaagg
       61 tagatcgcgc tctttactta atcagtcgcc cgcttcacaa aactcttgcg attgacttct
      121 aggctccacg ctgtttggtt ttcaaggaac atccgcggct ctcgccgcgc tccgccgggt
      181 gccctcgcat ccgggcggca aaagttatgt taccatacag gcctaattcg tgtcaactga
      241 aaaagatggt agttacgggg tagcatcaaa ggagcgctga agaaaccttg tcatgccttc
      301 aatgagcacc tgggtcggtt cacttccata tgcccctctt aaggtccgca acgcctccaa
      361 ctgccccgga tgcaagggga gtgtgggtca aagtaacgca gtcatcatcg tcccaattgg
      421 gcccttccac tttcgcgtta ttccatatgg taaccgtacc agctatgata gcgccctcta
      481 cctcgacgtt tgggtgaact atgacgctgc agttgggtgc atacacgatg ccttgtgtct
      541 tgcagttgtt atcaatgtcg actgttgctc cttctttgcg tacgaccagg ttgccttcca
      601 ttgtcacgtt gttcttcagt tcaaccgatg aatcgtccac gtgtatgtgc gctttgatca
      661 ctgagttgtt gagagcagca agggattcac accagattct caactgatcg ggttctccgt
      721 ctttgttcac ttcggtgttg ttgatgaagt ctatcgactt agcatatagc agaaccgttc
      781 ctgtgccatc aatggtgagc gtagccttgt tcttcagtat gaggtcgtct acagtgatta
      841 ccttggttgt tcccgccgga gctattatgg tccacttagc attcttcggt attgtcaggt
      901 tgccataggc caaatcgggc tcagtgggcc acgcaatcgg aagcgggttc ggatactcac
      961 gggcactatc aagactgact ataggcggct taggggtgat ggaggtgctc acaaccttat
     1021 tgggggtttc tgtaggccct gtcttgatcg tgcagtccat gatcttagct ttaccgatct
     1081 taacggcccc ctcagctgtg ctgttggtga ttatgtctcc atcaatctct gcgttattct
     1141 caatggttat tgggccatag ccaaatatcg ccatatcgac aggagcaggc tgaagtgccc
     1201 gtatggtgta ggtcactgtc tgagtcaccc gtcccgcttt acccacagac gtcacagcat
     1261 attccgccgc tcccatcttg cttaccgaaa gagtgaagga acctgtggcg aatgctgtct
     1321 cctgggagtt gcccttgtct accagttgct caaggaaagt cattttctca ccaatggtga
     1381 ggttttgcgg attatcaacg atataagaca ccagagcctc tgcaccagaa cgtgctatga
     1441 agtacgcctg tacacggttc ttctggcgta acgcttgaat gcgagaagac gtggctagtg
     1501 ctgatacggc gcctgctagc accatgagaa ccacaactac ggtgagtacc gtaaccaaag
     1561 cggcaccgtc ctgatcgcca agggagactc tcgtgcgata tgagatcttc aaacagatca
     1621 cctaccatcc tcgtgttgct cggactgcat cgctcgatgg gaaacaacga gttacgggag
     1681 ggtgaatcgt aggtacaatc ccgaatcagg cgaaaatgcc ccttccttga tgttggccag
     1741 atacgcgctt gtagataact tcgacgatct gcccttcgat gaggatgcaa gatcgatcgt
     1801 cagcacacag cccccggctg aactggccag agcgaactga acattttcca ctcgcgtacc
     1861 caccaaaggg actccgcttg cgccctcctt gtcatagaga agcgtcgaaa caccgccgga
     1921 atagcccaag tagatgcagt cagcaccttt gccggaggca tcatccgata ccgatacctc
     1981 aacagcattg cgcacctttg agacaatgct ttcggaggcc gcagtcagtg aggtctgcag
     2041 atccgcctgt gccaaggtca gcgcaaaggt acgttggcca aacacaaaca gcgtgacggc
     2101 gattgcgaaa accataccta cgagggtgat cgcaacgaga agctccacaa gggtggctcc
     2161 gcgctggcaa ctatgcataa acaccccatc ctaacgcagt gtgcgacgct gccttacgct
     2221 atgggatcca agtggagacc ggaacctctt ggctgcgaaa cgttgcacca atgtctatct
     2281 gcttaccggt aacgtagatc ggacctgttc cttcaaactg aagacacaaa gtcgccgaac
     2341 tgtccgagcc aaccacactt ggctgagttc cactacccgc cacaccctgc ggcgtctttg
     2401 caggtatgga cctcgacatg gcatcctcgg ccgcaaacac agctttcgta agtctttcgc
     2461 ccattaccac tgccccgaat atctggttga aagctgcgag aatggcaccg agcgaaacgg
     2521 acagcacgac tattgccacc aacacttcca tgagcgtaaa tcctcgctca tcactgactc
     2581 tcatcgctac cgtcctctca aaacacggat tgaatcattt atgttctcgg tatcagccgc
     2641 actctatctg accgaatacc gcctgggtat ccattcctcg tcacaggcga tatcgcctct
     2701 gcctacatga ggcttgaggt ttgatgaagt caactcttcg gtcacttcct ggacaactga
     2761 cacagacttg accttgtcct ttccgcgcca gctcgtgccc attcctgcgt ttatccctga
     2821 gtagaagaag gcgccgataa gcccaaggag aaccacgacc accacaatct ccacaagggt
     2881 aaaaccgtca tctccgacgt gggtaatcag gaacaccccc aggcgcagag tggatgaggc
     2941 actagactaa ggttattgta acaattatta cccattagga aggaatgtgc aacagccgag
     3001 taaatggagg caagagaatg cgacttagca gatcgccatc tgcgaggcgc gcaggaggcg
     3061 cggcgctacc taacccactc ccgcgaccaa tcagaaatct ccgtaggatc gtacccaggg
     3121 actcctgagt cgcctacggc gcggaatgtg actgtggtat tgttcttggc cttgaacgaa
     3181 ccgcccactg tgactgtacc tgccagttcc atgttgttca tgtcacgagt atctcccccg
     3241 acgactaggt gcagcttacc gttttcaccc ttgagcgaat ttttgcaaac caaatcgcca
     3301 accacaatca cggtgatatt gcccctaaac gtgaggttgt tcttcgcgag cagatcgccg
     3361 ttgacaaata gcactccgtc gcctgatggg ctctcgatcg taagatcgtt tattgagggc
     3421 tccccatgtt tgtcgcgctt tccgagtgtg ccgtcgaaga acctgtaccc gttcactatg
     3481 atgtggctgt cgtctcccga ggcaacctcg ggcgccgggt agcccctgta ct
//
LOCUS       DAOSYL010000114         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_21396_length_3501_cov_5.153221, whole genome shotgun sequence.
ACCESSION   DAOSYL010000114 DAOSYL010000000
VERSION     DAOSYL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21396_length_3501_cov_5.153221"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLU15_10735"
     CDS             <1..202
                     /locus_tag="PLU15_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31417.1"
                     /translation="LRFARSRVRGPYARFCERNDARLIAWRHPTRLSYLQVSYDLAAP
                     LVKAVRKGVAKLQTIGFIGAMC"
     gene            complement(282..1022)
                     /locus_tag="PLU15_10740"
     CDS             complement(282..1022)
                     /locus_tag="PLU15_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOO31418.1"
                     /translation="MRFLIDPEVFEVLPTMCIGVVVAHGIDNTQLQSDNAQKISALLS
                     KAMRRTRAKFAGTNVKEHPDIVPYREAFRALGYNPNKFPCSVEALTSRVVKGSDLPDI
                     NSVVNLVNAVSLELALPMGAHDLDSISGDLAVRFSRQGELFTPLGEAEPEEVPAGELV
                     YADGREIRTRRWIWRQNDRGKVTEESRNIFFPIDGFTDRNRDAVERAMAELAGRLEEF
                     FGVAAHQYFLDRDRNSCDLMASVEQKKA"
     gene            complement(1264..1431)
                     /locus_tag="PLU15_10745"
     CDS             complement(1264..1431)
                     /locus_tag="PLU15_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31419.1"
                     /translation="MTKADKKTQLFAVVLVGAEEGDIENPLVRATSSADAISAALHDP
                     RWEGYACTFGS"
     gene            1628..3085
                     /locus_tag="PLU15_10750"
     CDS             1628..3085
                     /locus_tag="PLU15_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO31420.1"
                     /translation="MVIHVVAPGETLWVISRRYGVSIAAIVSANAIADPNVLVVGEAL
                     AIPLPRPEPAPLTYVVRRGDTLAAIAGLFSTTVQSIALANQITDVNLVYVGQELVVPG
                     WTYQTYTVRSGDTLSRVAAAFSTSVQLIVAVNHIADPNVINVGQVLIIPARGLPAPPK
                     PLVETNGYIFLLSESTLRRILTPIAHDLTYISVFSFSVDGRGGLDYAPGRARAAVDVA
                     RSMGIVPLIVISNFDGTNFNPDLARDAMLEPTGVATIGAIVDVAVSEGFGGVNIDLEN
                     MYPEDRELYTAFMAALSDAAHSRKLTLSNAMAPKWADWPTLPWVGTFDYAALAPSLDF
                     TMLMTYEWGWSGGPPMAIAPVNLVRRVLDYAIGKIPANKILMGIPLYGYDWTLPAAAG
                     RLADTVTPAEATAIAARHGININFDDIGKAPWFRYIDQSGAEHEVWFEDVRSSVAAHS
                     LVGEYGLRGTSYWNLLFDFPQNWLALEDRYIVRKL"
BASE COUNT          742 a          954 c          953 g          852 t
ORIGIN      
        1 actaaggttt gccaggagcc gtgtacgggg cccgtacgca cggttctgtg agaggaatga
       61 cgccaggctg atcgcctggc gccaccctac tcgattgagt taccttcagg ttagttatga
      121 ccttgctgct cctttggtga aggcggtccg aaaaggagtg gcaaaacttc agacaattgg
      181 cttcatcggg gctatgtgct gactcaactt tcgtgttctg acaacagagg cccatagcga
      241 gcctgtcttg agaagcccga gatccacttt gtccttgcga atcaggcctt tttttgttcg
      301 actgaggcca tcaagtcgca cgagttacgg tctcgatcga ggaaatattg gtgcgctgcc
      361 acaccgaaga actcttcgag tcggccggcg agttcggcca tagcccgttc aacagcatca
      421 cgattgcggt ctgtgaaccc atctatggga aagaagatgt tccttgactc ctctgtcact
      481 tttccgcggt cgttctgccg ccatatccat cgccttgtcc ttatttcccg tccatcggca
      541 tacaccaact ctcctgccgg gacttcttcg ggctcagctt caccgagcgg ggtgaagagt
      601 tcgccttggc gcgagaatcg caccgcgaga tcgccggaga tggagtcgag gtcgtgcgcc
      661 cccattggca acgcgagctc gagcgacacg gcgtttacaa ggttcacaac ggagtttatg
      721 tcgggaagat cgctcccttt gactacccgg gatgtgaggg cctcaaccga gcaggggaac
      781 ttgttcgggt tatatcccaa cgcccgaaat gcctctcggt agggtacgat gtcaggatgc
      841 tccttcacat ttgtccccgc aaacttggcc ctagttcggc gcatagcttt actgagaaga
      901 gcactgattt tctgagcatt gtctgattgg agttgggtat tgtcgatgcc gtgcgccaca
      961 acaacgccaa tgcacatagt gggcaatacc tcgaatactt caggatcgat cagaaatctc
     1021 atgtgccgat tcctcctgtg tctgccagat aggcggtcgt aagcatttca gcggctatgc
     1081 gttgcaattg cccatgcggg gcgttctact taatgataca gcaaatacga gtcaaaccgc
     1141 cacatacgtt tgaaggctcc ttcggattgc ccaggcccaa tggaatctgg gccttttctt
     1201 tgctcaggat cggaggttag atctatctgc ccaggccgtc atcgcgctca atcgagacca
     1261 atgtcaagag ccaaaggtgc atgcgtatcc ttcccatctt gggtcgtgca gagctgctga
     1321 gatggcgtcg gcagatgatg ttgcacgcac cagagggttc tctatatctc cttcctcagc
     1381 gccaacaagg actacggcga acaactgagt cttcttatct gccttcgtca tgttactacc
     1441 cccccccgtt caagtggcat gctctgacat acaaacacga aggacgaagg attgcacctt
     1501 cccgaagaat tcgaaccgca gctacactta tcaacggcgc gagattagga cgacgaacgg
     1561 tcgaaaacgc aaatgctgca ggagagccat atgatgggat gactgatttc ccatgggagt
     1621 gatttgtatg gtgatccatg ttgtggcacc cggagagact ctctgggtga tttcgcgcag
     1681 atatggtgtg tctatagctg caatcgtctc ggcaaatgcc atcgccgatc cgaatgtact
     1741 agtggtgggc gaggcgcttg ctataccgct gccgcgccct gagcctgcgc ctcttacgta
     1801 tgtagtgagg cgtggcgata cattggctgc gattgccggg ctcttctcaa ctactgtcca
     1861 gtccatagct ttggccaatc aaataacgga tgtcaacctg gtctatgttg gacaggagct
     1921 tgtggtgcct ggatggacat atcaaacata cactgtgagg tccggcgaca ctctatcacg
     1981 cgtggctgcg gccttttcca ccagcgttca gttgattgtg gcagtcaacc acatagctga
     2041 ccctaatgtc atcaatgtcg gccaggttct gatcataccc gccagagggt tgcctgctcc
     2101 gcccaagccc ctcgtggaga ccaacggcta catcttcctc ctttccgaaa gcactttgcg
     2161 ccgcatcctg acgcccatcg cccatgacct cacgtacatt agcgttttca gcttctccgt
     2221 agatgggcgg ggtggtcttg attatgcgcc cggtagggct agggcagcgg tggacgtggc
     2281 ccgttcgatg ggcattgtgc ccctgatagt catctctaac ttcgacggga ccaacttcaa
     2341 ccccgacctt gccagagacg ccatgttgga gcctacaggc gtggcaacta taggggccat
     2401 cgtggatgtc gctgtatccg aaggttttgg aggcgtcaat atcgaccttg agaacatgta
     2461 tcctgaagac agggaactat acacagcatt catggctgct ctctcagacg ctgcccactc
     2521 ccgcaaactc acgctttcca atgctatggc gccgaaatgg gctgattggc ctacgctgcc
     2581 ctgggtgggc acatttgact acgcggcgct ggcgccttca ctcgatttca ccatgctcat
     2641 gacttatgag tggggttggt cggggggacc ccctatggcc attgctcctg ttaatctggt
     2701 gcggcgcgtg ttggactacg cgattggcaa gatccccgca aacaaaatac tgatggggat
     2761 acctttatac ggctatgatt ggacgctgcc ggcggccgcc ggaaggctgg cagatacggt
     2821 tacgccagcc gaggccaccg caatcgccgc gcgccatggc ataaacatca acttcgatga
     2881 catcggcaag gctccatggt tccgctacat tgatcaatca ggcgcggagc atgaagtatg
     2941 gttcgaagac gttcgttcgt ctgttgccgc gcattcactt gtaggagaat acggtctgcg
     3001 gggcacaagc tattggaacc tgttgtttga ctttcctcag aactggttgg cactcgagga
     3061 ccgctacatt gtgaggaagt tgtgagagag ttgtgggcgg cctatgccgc ccatcgtaca
     3121 gaggaaatgt ctgatcccgg agctttgcat caccggtatc gccgtaagag ctagtcgact
     3181 accaggtcgc ggcccgcggc aaagcccatc gcgatctcgt tgccatccga gatccccagt
     3241 tttcgcagga tgtataaggc aacactgcag ctggttgacg agtaactcaa gccgactcat
     3301 tgtcatggga gatgacggac cgctgcgctg ctaatatctc atcgatgggc ttgccgttga
     3361 tggttgcgaa tttcattgtt gattgtgttc cggtattcct ctcgggcatc gaatgcatat
     3421 atccctcatt gaactagtag agccccttac gggatatgtg aagcggaacc ccgagcttga
     3481 ccatctgttc tatataggtg t
//
LOCUS       DAOSYL010000115         3498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_21417_length_3498_cov_8.102236, whole genome shotgun sequence.
ACCESSION   DAOSYL010000115 DAOSYL010000000
VERSION     DAOSYL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21417_length_3498_cov_8.102236"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..368)
                     /locus_tag="PLU15_10755"
     CDS             complement(159..368)
                     /locus_tag="PLU15_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31421.1"
                     /translation="MDKDVYYPDREELELHRLYRVLDFLISHKDVIEDHAFYNLTDVF
                     PMDVSVLVYGCSLVDMYGESSRRCR"
     gene            complement(466..1266)
                     /locus_tag="PLU15_10760"
     CDS             complement(466..1266)
                     /locus_tag="PLU15_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO31422.1"
                     /translation="MFERYFGFTRTPFARDLKPSDLFDWSGAKEMRSRLNYVAERKLF
                     GVFTGNVGAGKTTIVRWFVEELPRAKYKTLYIHDSELTPRNLYWECLNQLGIKPRFYR
                     GDAKRQLNAVLVDMAEGQGKTPVVIIDEAHLLSHQMLEEIRFLTNFRMDSYSPMSLIL
                     VGQTELLAALQRPSLLAISQRVSIRWHLPYFDLSETTRYIEHNLNIAGVTTPLFTDGA
                     IKVIHEATQGAARAIGGLSCSCLLHAMSTNAKLVDDHSVKLVLENEYR"
     gene            complement(1259..2593)
                     /locus_tag="PLU15_10765"
     CDS             complement(1259..2593)
                     /locus_tag="PLU15_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOO31423.1"
                     /translation="MKYDREIEKALSRYAAIAPVAKEGMSRAEACEGWTEVREARARN
                     VDGADVSMRTLRRWATAYHDEGFEGLQRKERGDKGQLRAMNAAVLDRAIALKEELPSR
                     SVRQIIDIMTAEGTIEPGEIARSTLSRHLKEAGCMDLPKKSPKATRRFVKERPNQLWQ
                     GDLKYGPYIPDSTGKPRRTYLIAFIDDCSRLIPHAQFYFDQKGPSLEDCLKKAIIKRG
                     VPEAIFVDNGKIFVSRQLRVACATLGVRHLTAAPYSPEAKGKIEKWLGFVQSSFINEL
                     KVSPAMCLEGLNERFGAWVEEFYHNRPHSSLDGKSPLEVYRSSIKNIRTETYEAITEA
                     FKHSAERKVDHTGCFSFEGQTFRCGVDHAGKRVLLRYDPSDLSSVEVFYKNERVGIAT
                     PIKVCVQGGSDKQTSGPDSVSFDKLMVERQQQSLERRLGAISFSGLEEDKDV"
     gene            complement(2673..3206)
                     /locus_tag="PLU15_10770"
     CDS             complement(2673..3206)
                     /locus_tag="PLU15_10770"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HOO31424.1"
                     /translation="MEEIRRRVREGLIECPVCRAKLNLRGGYGRKIRGGGVGVEPRVV
                     RCECPGCGTCHSIIASVMSPRSPYTQEIREKVVRELKRGKPRKQLSRELGICESTLRN
                     WVKAFDTKQKSIAATMSALLVALREKPHGPFVEQVRRAAKALNLKLLCNLVFAQTNVL
                     LSLAQWMGLGALSVSWL"
BASE COUNT          750 a         1018 c          856 g          874 t
ORIGIN      
        1 gtaaccaacc cgcggatatg agtctgccaa ccgtcgacgt aaaccgggtc ttcggcggcg
       61 ccactaccga aaccaaactc ggggcttgac ggccttattc atatgagttc tccaacccct
      121 ccccaagctc gaatacctct cgcatagcct gtacgttcct agcggcatcg ccgggagctc
      181 tcgccgtaca tatcaacaag gctacagccg taaacgagta cgctgacatc catcggaaag
      241 acgtccgtaa ggttgtagaa agcgtggtcc tcgatcacat ccttgtgaga aatcaagaag
      301 tcaagaactc tgtacagacg atgcagctca agttcctctc tatcaggata gtagacatct
      361 ttgtccatcc atgcctgtcg atgcttacgc tttttgtccg cgtagatccc gagttaattg
      421 gccacaggaa agagcacgac agcttccgct gtggccggtc agttgctacc tgtattcgtt
      481 ttcgaggacg agcttgactg aatgatcatc aacgagtttt gcgttggtgc tcatcgcatg
      541 cagcaggcag ctgcatgaga gcccgcctat ggcgcgcgcc gcgccctgtg tagcttcatg
      601 gataaccttg atagccccat cagtgaagag aggcgtcgtt acccctgcaa tatttaagtt
      661 gtgctctatg taccgggtcg tttccgagag gtcgaaatac ggcaagtgcc atctgatgct
      721 gaccctctgc gatattgcaa ggagcgatgg cctctgcaag gcggccaaaa gctctgtttg
      781 gccaaccaag atcaagctca tcggagaata gctgtccatc ctgaagttcg tgagaaagcg
      841 tatctcctca agcatctgat ggctgagcaa atgggcttca tcgatgatca ccaccggggt
      901 ttttccctgg ccttcagcca tgtccaccaa cacggcgttc agctggcgtt tggcgtcccc
      961 acgataaaac cgaggtttga tcccaagctg gtttaggcat tcccaataga ggtttctcgg
     1021 cgtaagctcc gagtcatgta tgtatagcgt tttgtacttg gcccggggca gttcttctac
     1081 gaaccagcgg acaatggtcg tctttcctgc cccgacattg ccggtgaaga cgccgaatag
     1141 tttcctttcg gcaacatagt tgagccttga gcgcatttcc ttggctcctg accagtcaaa
     1201 caggtcagag ggtttgaggt ctctggcgaa cggagttcgg gtaaaaccga agtagcgctc
     1261 aaacatcctt atcctcctcc aagcctgaga aggatattgc gccaagcctg cgctctaggc
     1321 tctgctgttg ccgctccacc atcagcttgt cgaagcttac ggaatcgggc ccagatgtct
     1381 gcttgtcgct gccgccttgc acacagacct ttatgggagt tgcaatgcca accctctcgt
     1441 tcttatagaa cacttccact gagctgaggt cagaagggtc gtagcgaaga agcacccttt
     1501 tgccggcatg atccacgcca catctgaagg tctggccttc aaaggagaaa cagcctgtgt
     1561 ggtctacctt gcgttcagcc gaatgcttga acgcctcagt tattgcctcg taggtttcgg
     1621 tgcggatatt cttgatgctt gagcggtaga cttccaatgg ggacttgcca tcaagggatg
     1681 aatgaggccg gttatggtag aactcctcga cccaggcgcc aaatctttcg ttaagccctt
     1741 ccagacacat ggctggcgaa accttaagct cattgatgaa cgaggactgt acaaatccta
     1801 gccatttctc gattttgccc ttcgcctcgg gggagtatgg ggctgcggtc aagtgtctaa
     1861 cgcccagcgt agcgcaggca acacgaagtt ggcgtgatac aaagatcttg ccgttatcca
     1921 cgaagatggc ctccggaacc ccccgcttaa tgatcgcttt cttcagacaa tcctcaaggc
     1981 tcgggccctt ttggtcgaaa tagaattggg catggggaat cagtctggag caatcatcta
     2041 tgaatgcgat caggtaggtc cttcttggtt tgcctgtgct gtcgggaatg tacggcccgt
     2101 acttgagatc gccctgccag agctgatttg gccgctcctt tacgaacctt cgggttgcct
     2161 tcggcgactt cttgggaaga tccatgcacc cggcttcttt gaggtgtcgg cttagggtgg
     2221 aacgcgcgat ttcgcctggc tcaatcgttc cctccgcagt catgatgtct atgatctgtc
     2281 ttacgctccg gctaggaagc tcctctttga gcgcgatcgc gcggtcaagg acggcagcgt
     2341 tcatagccct gagttgtccc ttatcccccc gttctttgcg ctgcagcccc tcaaaacctt
     2401 catcgtgata ggcagtcgcc caccgcctca aggtgcgcat ggaaacatca gcgccatcaa
     2461 cgtttcttgc cctagcctcc cgaacctctg tccatccttc gcatgcttcc gcgcggctca
     2521 tgccctcctt tgccactgga gcgatcgccg cataccttga aagtgctttt tcgatctctc
     2581 tgtcgtattt cacttttagt tcctcctcga gaagaagtca ctaaaagctt atcctcagaa
     2641 catctcgcgc ggaaggttgc atctcatggc ggtcacagcc atgagacact caaagcccct
     2701 aaccccatcc attgcgccaa gctcagaagg acgtttgtct gtgcaaacac caggttgcac
     2761 aaaagcttca aattcagggc tttagctgct cgtctcacct gttcaacgaa gggcccgtga
     2821 ggcttctccc tcagcgcaac caggagggcg ctcatggtcg ccgctatcga cttctgcttt
     2881 gtatcaaaag ctttgaccca gttgcgcaaa gtgctttcgc atatcccaag ttccctgctg
     2941 agctgtttgc gcggctttcc ccgtttgagc tcgcgcacca ccttctcccg aatctcctgg
     3001 gtgtaggggc ttcttgggga catgacagaa gctattatcg aatggcaagt cccgcacccc
     3061 gggcactcac accggactac cctcggctca acccccacgc ccccgccccg aatcttgcgg
     3121 ccatagccgc ccctgagatt cagctttgcc ctgcaaacag ggcattctat cagtccttcc
     3181 cgaactcgcc gtcgtatttc ttccactacc ccagcatcat agctcttgaa atctttgcag
     3241 caatctatta cgatcaagat gtttcgtggg gatgaagtgg tcccggggag aggctagaaa
     3301 ctcgtcgatc cggtacccct tcatctctct cctttcatgt gatgtttccc cattctacac
     3361 caccacccca acccctttcc attaatgaaa tttaatctga gcaaaaaccg aatctacgcg
     3421 gtcatctagc gtgagcccct acacaattgc atggactttt tgatggggac cgtgggttag
     3481 caaaccttct tgaatcag
//
LOCUS       DAOSYL010000116         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_21700_length_3455_cov_9.255000, whole genome shotgun sequence.
ACCESSION   DAOSYL010000116 DAOSYL010000000
VERSION     DAOSYL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21700_length_3455_cov_9.255000"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1476)
                     /locus_tag="PLU15_10775"
     CDS             complement(118..1476)
                     /locus_tag="PLU15_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO31425.1"
                     /translation="MKRSDVVKRVDIDHIREIERESNRLLNEKKWAEAEMVLRKALEV
                     TPILVPAINNLAFCLFMQDNPEEALEVLRPNLSADVPNPFASALASQICVALGRMDEA
                     EKHLETAVRSFDAGLPQMRLAPSAISNPWYEYTVMLKRAAGDLGRHQQVIDMYRRHEA
                     YHTTPEDRYLAGVASFNLGRMGRAISYWSKLLWQLGLDFAKVAQSVEMGMIPPFSLEY
                     RVPFFDGNTPDMASGIGHMMLSHALLEGELDNEDRSTAYSMLDLAVKEDRDWGLQLAE
                     NILQYPAVSDKVKSAALSILVNYGVCNEGQAVDVVGQDGEPITISLKKITMTCNIAED
                     EREIIREAARLAENDEFDEAIAILEEAITNSQEISLYMLLAQAATYTYKGDLDKAYEI
                     LSMLRDAAPDDPLVLFRLAEYYCESGNAQEALRCLDHIELEDVSPTLQRGIANIRESL
                     YE"
     gene            2065..3306
                     /locus_tag="PLU15_10780"
     CDS             2065..3306
                     /locus_tag="PLU15_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO31426.1"
                     /translation="MANVVERAVQFISRQPRNWKVTACRASIDRCVYQMVFPYLSVYI
                     ALLGATGTQLGAINSAGMVASAVLGLLAGTAVARVGTKKVYSAGVALVALSYLIIGLA
                     QNWVLALAGTMLWWCGSTAAGYGCAIVCGTSLANRDRATAMGCCESLTQGVMGFIGPV
                     IGAMLVAGFGGVSVRGIRPVFFIASIMCLGAWLFVSTQLSETECGIGNGGSNPFLLLR
                     DRPDLVRFVAVSCLTSLPTGMVLPFTQVYASQVKMADQYVLGTMVTAAAVIGLLLGVP
                     LGRLADRMGRKRILFVLAPVYWIGNLLLVWSSRPASLVLVGVFQGAFTMTSMLTQAIA
                     VELAPSERTSDWLAVTKFFKMVAVAAMASVSGLIWDHVGPEYVFLLAVAIDAAVRIPL
                     LLGVPETLELPGRSGESSVRG"
BASE COUNT          726 a          855 c         1000 g          874 t
ORIGIN      
        1 ggatttcagg gccctatcgg tatccatcgt ggactgcttc tccccaggaa tggatttgcc
       61 ctcaggctcc ttggcccgct tttggggcat gttcagacct cagtttcaac tcacgattta
      121 ttcgtagaga gattcccgaa tgttggcgat tcctcgctgc aatgtcgggg acacgtcttc
      181 gagctctatg tggtccaggc aacgaagtgc ttcttgtgca ttccccgatt cacagtagta
      241 ctcggccagg cggaatagaa ccaaggggtc atcaggcgcc gcatcacgaa gcatgctaag
      301 gatttcatag gctttatcaa gatcgccctt ataagtatac gtagctgcct gggcaagaag
      361 catgtaaaga ctgatttcct gagaattcgt tatcgcttct tccaagatag ctatggcctc
      421 atcgaactcg tcgttctcag cgagcctggc tgcctcgcgg ataatctccc gttcgtcttc
      481 ggcgatatta caggtcatgg ttatcttttt caatgatatg gtgattggtt ccccatcttg
      541 acccactacg tctacggctt gaccttcgtt gcaaacgccg tagtttacca ggatgctaag
      601 ggcagccgat ttcaccttat cggatactgc tggatactgg aggatgttct ctgcaagctg
      661 cagtccccaa tcccggtcct ctttcacagc cagatcgagc atggagtaag cagtgcttcg
      721 atcctcattg tccaactctc cttcgagcag agcgtgggag agcatcatat ggccaatgcc
      781 tgaagccata tcgggggtat tgccatcgaa aaaaggcaca cgatactcga gcgaaaatgg
      841 gggaatcatc cccatctcta ctgattgggc taccttggca aagtcaagcc ccaattgcca
      901 gaggagcttg ctccagtacg aaatggctct gcccattcga ccaaggttga atgaagcaac
      961 gcctgcaaga tagcgatctt ccggtgttgt gtgataggct tcgtgacgac ggtacatgtc
     1021 gatcacctgt tgatgtcgac cgagatcgcc agctgctctt ttgagcatga ctgtatactc
     1081 ataccaggga tttgaaatcg cactcggggc cagacgcatt tgcggaagac ccgcatcaaa
     1141 actgcggaca gcggtttcca ggtgtttttc ggcttcatcc atgcgcccaa gcgccacgca
     1201 tatttgggac gcgagcgcgc tagcgaaggg gttagggacg tcagccgaaa gatttggtcg
     1261 gagtacttca agtgcttctt cggggttgtc ctgcatgaag aggcaaaaag ccagattgtt
     1321 tatggcgggc accagtattg gcgtcacttc cagcgccttc ctgagcacca tctcggcctc
     1381 agcccatttc ttctcgttca gcaggcgatt gctttctcgt tcgatctctc gaatgtgatc
     1441 tatatctact cttttcacga catcgctccg tttcatcagt gaggttcgcc aatgcagtaa
     1501 aggcactgtg gcatacagcc tatgttcata agtctacttg atattctgcc ccgctgccaa
     1561 tctggtttca aacaattcgc tttccaacgg tgggcccaag cttgcccggc tgagagaaat
     1621 caatccctac atgcaggcag gggaagggcc gatttcccag tctgagagtg ccagtgctca
     1681 atacgttgct cgtggatata acactcatag actagacagg cacagtgagg ttgttttccc
     1741 gtaataaggg ccggacattg tcattgccct gccagcgtac acgcggtaaa gaagctgttg
     1801 gctagtttga ggaatgggga aaaggatgct gaaaagttct ggtttgaagg cgagaagagg
     1861 ttgattgaag aataatgagg ctgtcgactg aacgtggggg acatagatgc ggaaattgct
     1921 tatttcttgt taggcgaaat gcaatactca gactggacgt gcggttttcc atgtcatgac
     1981 gagttgtgcc ctcttgtggg agatcggcgc ggcagggaag agaagtgtct gtgatgggcc
     2041 atgggccggt gaggggatga atgggtggct aatgtggttg agcgggctgt gcagttcata
     2101 tcgaggcagc ctcggaactg gaaagttaca gcatgcaggg catcgattga caggtgcgtg
     2161 taccagatgg tcttcccgta tctcagtgtc tacatcgcgt tgctaggggc cacaggcacc
     2221 cagttgggcg cgatcaacag cgcagggatg gtcgcaagcg cagtgctcgg tcttttggct
     2281 ggaaccgccg tggcacgagt cggcaccaag aaagtgtatt ctgccggcgt ggcgctggta
     2341 gccctttcgt atctgataat agggttggcc caaaactggg tattggccct ggcaggcact
     2401 atgctctggt ggtgcggatc taccgcagca ggatatggct gcgcaatcgt gtgcgggact
     2461 tcgctggcaa acagggaccg ggcaactgcg atgggctgtt gcgagagcct tacacaggga
     2521 gtgatgggtt tcatcggccc agtaatcgga gccatgcttg ttgccgggtt cggaggggtg
     2581 agcgtgaggg gaatcaggcc ggtgttcttc atcgcttcca tcatgtgcct cggggcttgg
     2641 ctttttgtgt ctacccagct gtcggagacg gaatgcggca tcggcaatgg aggcagcaat
     2701 ccttttttgc tgcttcggga taggccagac ctagtgcgtt ttgttgccgt gtcatgcctc
     2761 accagcttgc caacgggaat ggtgcttcca ttcacccagg tgtacgcgag ccaggtgaag
     2821 atggcggatc agtatgtgct cggaacgatg gtgaccgcag ctgcggtgat aggcttgttg
     2881 cttggagttc ctctggggag gctcgcggac aggatgggcc gcaagagaat actcttcgtg
     2941 ttggcaccgg tctactggat cggcaatctg ttgcttgtgt ggtcatcccg tccggcctct
     3001 ctcgtgcttg taggcgtgtt ccagggtgcg tttaccatga caagcatgct tacgcaagca
     3061 atagctgttg aactggcgcc atccgaaagg accagcgatt ggctggctgt gacgaagttc
     3121 tttaaaatgg tcgccgtggc cgccatggct tctgtttctg ggcttatatg ggatcacgtg
     3181 ggccctgagt acgtattcct attggcagtc gcaatcgatg cagccgtccg aatcccgctc
     3241 ttgctgggcg tgcccgaaac gctcgaactg ccgggccggt ctggggaaag cagtgtgagg
     3301 ggatgattcc ctgggctgtt cgacatggat tgaggtcaac ttactcacgc cctaaccagg
     3361 tctcgataag tctctgcgcg agcttcgagt ctgtgctgcc tgcagtttga gcacctgatt
     3421 aaggaatgct tcaaaaagtc catgcaattg ctctt
//
LOCUS       DAOSYL010000117         3413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_22009_length_3413_cov_8.539309, whole genome shotgun sequence.
ACCESSION   DAOSYL010000117 DAOSYL010000000
VERSION     DAOSYL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22009_length_3413_cov_8.539309"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLU15_10785"
     CDS             <1..1091
                     /locus_tag="PLU15_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO31427.1"
                     /translation="GLTSAAVTNIVSNFIEQGFVREVGYGRSGGGRKPKLVELDKGSR
                     IMVAVDLAGADQIAALLDMDGNVLCRGERRIGSAKGVTVADIVDAINEILDTPEARSG
                     QVVGIGVTTPGLVDAATGTVLKSVALNWFNVPIKAALQKHFEYPVFIGKDTHVALLAE
                     EWYGAAKNMQNAIYLWVGPGIAIGMLLDGRVYTGSTGMAGEFGHVSIDYDGPACKCGS
                     WGCMEQMASLRAISSMAAKSDRDAEGYSGDPEERYIDPFNVLDAAEAGDANARRIVHR
                     AGEYLGVGIANLINVFNPELIILGGQIRPSDTEFIEAARSSAMSRVLIEVGSAVTITA
                     SKFGRDAGLVGASALLWKEILRDLAESE"
     gene            1153..2076
                     /locus_tag="PLU15_10790"
     CDS             1153..2076
                     /locus_tag="PLU15_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO31428.1"
                     /translation="MADSGRMAVGVDIGGTHLRVGAVRGYEVLAYEKERAWSSDMGPD
                     EVMDIVAEHVRTVVDVAGLDMNEVEGVGVGIPGRVDHESGEVIQAVNASWARETVNVK
                     RMLELRLGLPVMVDHDVAVAAAGEYIYGGHGKSDYFLYVTLGTGIASCYIANGKLLRG
                     AHNSASEMGHTCIDPDGPRCVCGMAGCLERLAAGPAIARAAGMRSEEVVEAARNGEPR
                     AVDVMDRVVNYLALGFVNVINVLDPDVVVIGGGLSQVEDVIVDPLRMRIRKIYRSPFA
                     EELEIKRATFGDDAGIIGAAAQFGQEYADLS"
     gene            2196..>3413
                     /locus_tag="PLU15_10795"
     CDS             2196..>3413
                     /locus_tag="PLU15_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO31429.1"
                     /translation="MTCELDRLIGLDGAEKERLGIIFTPSEIRSQASLWRESGETVFN
                     KQTELSGLFVPARPGRPGLHDPFDPCHRRTLILSGAGSSGHVGRCLAPLFRRRLNVHA
                     SVFDTTDIVIDPEGHLIKDEPCLVVLFARSGDSPESLATYEIARSYCKDVRFLAVTCN
                     PEGGLARLAAERPSEVSLILLDPSCNDKSLAMTSSFTNMVVAGQALSFLDAPAEYRKQ
                     AELMHTAAEDLIQEDSVFRELAETPFERAVFLGDGALYGAAAESALKVQELTDGTVMT
                     MSQTFLGVRHGPKAVVNNKTLVVFYVSTDSYKLEYEADLIEQFCRENVAMKTMAVAPN
                     LPKRISDLVNCSVSYGQKAQGEIDDDLRSPIDVIAGQLLGVYKALDLGFKPDAPSARG
                     VISRVVKGVTIYPF"
BASE COUNT          752 a          767 c         1105 g          789 t
ORIGIN      
        1 cagggcttac ttctgccgcg gtcaccaaca tcgtcagcaa ctttatcgag cagggtttcg
       61 tgcgtgaggt tggttatggg agatccgggg gaggacgcaa accaaagctg gtggagcttg
      121 ataagggatc aagaatcatg gtcgcagtgg acctcgccgg ggcagatcag atcgcggcgc
      181 tcttggacat ggacggcaac gtgctttgcc gtggagagcg gcgcatcggg tctgcgaaag
      241 gagtcactgt tgccgacata gttgacgcca taaatgagat actggatacc ccggaagcgc
      301 gctcaggcca ggtggttgga atcggtgtta ccactcctgg tctggttgat gctgccactg
      361 gcacggtgct caaatcagtc gctttgaact ggttcaatgt gcctatcaag gcagcccttc
      421 agaagcattt cgaatatccg gtgttcatcg gtaaagacac ccatgttgcc ctccttgccg
      481 aggaatggta cggtgcggcc aaaaacatgc agaacgcaat ttatctttgg gtggggccgg
      541 ggattgccat cggcatgctg ctggatggac gggtttacac aggttcgact ggaatggctg
      601 gcgagtttgg gcatgtaagc atcgactatg acgggccagc gtgcaagtgt ggaagctggg
      661 gttgtatgga acagatggcc agcctaaggg cgatatcttc tatggctgca aaatccgaca
      721 gagacgcgga ggggtacagc ggcgaccctg aagagcggta catcgatcca ttcaacgtgt
      781 tggatgccgc tgaagctggc gatgccaatg cccggaggat agtgcatcgc gctggcgagt
      841 atttgggtgt tgggattgct aacctgataa acgtcttcaa ccctgaactc ataatcttgg
      901 gagggcaaat tcgtccctcg gacaccgagt tcatagaagc ggcaaggagc tccgcgatga
      961 gtcgggtctt gattgaggtg gggagcgcgg tgactattac agcttccaaa ttcggacgtg
     1021 acgcgggcct agttggagca agcgccctgc tgtggaagga gatattaagg gaccttgccg
     1081 agtcagagta gttctgggac atgttctgaa ttggtcccac tggttacagc tcaggtgtgg
     1141 gagtgatttg agttggcgga tagcggacgg atggcggtgg gggtagacat tgggggcact
     1201 catctccgcg tgggagcggt gcgtgggtac gaggtgttgg catacgagaa agagcgtgca
     1261 tggagcagcg atatgggtcc cgatgaggtc atggacatcg ttgccgagca tgtgagaacc
     1321 gtggtcgacg tcgcagggct cgatatgaat gaggtggagg gtgttggcgt agggattccc
     1381 ggtcgtgtgg accatgagtc cggcgaggtt attcaggcag taaatgcaag ctgggctagg
     1441 gaaacagtca acgtaaagag aatgttggag cttcgcctgg gtctgccggt gatggtggat
     1501 catgatgttg cggtggccgc tgcgggagag tacatatacg gaggtcatgg caagagcgat
     1561 tactttcttt atgtcaccct tggaaccggc atagcttcct gctatatagc caacggcaag
     1621 cttctgcgcg gcgcgcacaa cagcgcctct gagatggggc atacatgtat agatcctgat
     1681 ggccctagat gcgtctgcgg catggcggga tgcctcgaac gtcttgctgc cggtcctgcc
     1741 atagcgcgag cggcggggat gcggtctgag gaggttgttg aggccgcccg gaatggagag
     1801 ccccgggcag tggatgtaat ggacagggtc gtgaactatc ttgctttggg tttcgtgaat
     1861 gtcataaatg tgctggatcc ggatgtagtg gtgatcgggg gagggctttc tcaggtggaa
     1921 gatgtgatag tcgatccttt gagaatgcgc atcagaaaga tctacaggtc accatttgcg
     1981 gaagaattgg agatcaagag ggcaacgttt ggcgacgacg caggcattat tggagctgcc
     2041 gctcagtttg gacaggaata cgcagacctt agctagggtc ttcatgtgct tccaagcttc
     2101 ggatcgcagc gtgtggagcg gtttgctgag cgcgcccacc ctgtacggcg tagccgaact
     2161 gacggctaga tcagtttggg ggagaggatg catccatgac gtgcgaactt gacagattga
     2221 tcggtctcga tggcgccgaa aaggagcgct tgggcatcat atttactcct tctgagataa
     2281 ggagccaagc gagtttgtgg cgagagagcg gggaaacggt cttcaacaag cagactgagc
     2341 ttagtggcct gtttgtgccg gctcggcctg ggaggccggg gctacatgat ccgttcgatc
     2401 catgccatcg gcggactttg attctctcag gcgctgggag ttcggggcat gtggggaggt
     2461 gccttgcgcc gcttttcagg agaaggttga acgtgcacgc aagcgtgttc gacaccaccg
     2521 atattgtgat tgatcctgaa gggcatctca tcaaagacga gccttgtctt gtcgtgctct
     2581 ttgcaaggtc aggagatagt cctgaaagct tggcgacgta tgagattgcc cgatcctatt
     2641 gcaaggacgt ccgatttctt gccgtgacgt gcaaccccga aggaggcctg gcgcggcttg
     2701 ctgcggaacg cccgtcggaa gtttccctca ttctgctcga ccctagttgc aacgataaga
     2761 gccttgcaat gacgtcgtcc tttaccaaca tggttgtagc aggtcaggca ctgtctttcc
     2821 tggatgcgcc tgctgaatat cgaaagcagg cagagctgat gcatactgcg gccgaggatc
     2881 tgattcaaga agatagtgta tttcgtgaac tcgcggagac accgtttgaa cgtgccgtgt
     2941 tcctaggaga cggggcatta tatggagcag cggcggaaag cgctctcaaa gtgcaagagc
     3001 ttacagatgg gactgtcatg acaatgagtc agacattcct cggtgtcagg catggaccta
     3061 aggctgtagt caacaacaag actctggttg tcttctatgt ttcgactgat tcctacaagc
     3121 ttgaatacga agccgacctc atagagcaat tctgtcgaga gaatgtggcc atgaaaacga
     3181 tggccgttgc tcctaacctt ccaaagcgca tatccgattt ggtgaattgt tcggtaagtt
     3241 acggtcaaaa agcgcaaggg gagatcgatg atgatcttcg gtcgccgatc gatgtgatcg
     3301 cgggtcagct attgggagta tacaaagctt tggacctagg attcaagccc gatgctccat
     3361 cggcgcgcgg ggttatcagc cgtgtagtga aaggggtcac catttaccca ttt
//
LOCUS       DAOSYL010000118         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_23190_length_3268_cov_7.797386, whole genome shotgun sequence.
ACCESSION   DAOSYL010000118 DAOSYL010000000
VERSION     DAOSYL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23190_length_3268_cov_7.797386"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..2282
                     /locus_tag="PLU15_10800"
     CDS             219..2282
                     /locus_tag="PLU15_10800"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOO31430.1"
                     /translation="MILSRSRKPFGNISSSSPESSTDWVQCTFWAMLLSSIILSTVIV
                     RANHLCREVKNNLLSRATTAAALIDPAILSNLEEAFSLLLRPCSNRVASPNQTRPSLT
                     AAYIVSLDSQRIRFISGASAQPGSSNTLPNPGDVIGRASPEEVALIFAGHPIVRGPYP
                     SGRSKSVTAYAPVIDTRTGTVVAALALEVDAADCPEPLAKGWSVIAFALALTGTLGIA
                     LPRMHAENEATEQLRTASHDQLTGLANRSALISEIERAIREAGTDLQSTLLLIDIDDF
                     RTLNDTLSYGVGDDVLVQVAQHIRDEVRREDFVARFGEDAFAVLVRTPAIEQAVVTAE
                     RIRASLEHVEVETGGTAITVYLTVSVGIAEIDANSSVKDVALRADSALCAAKSAGKNR
                     IVCSSVAAGGRDEPDAYTQIAMIEDALEHDKFILHFQPIVPIVPINPNRGSKNHFADQ
                     DHALKWVGYMDGSRLHEALVRIEGPNGEIIPPGKFIPWAEAIGLVAQIDLWVLEHAIE
                     VLNERPDMNVAINLSARSISRPSFLTQVEQRVASSGLAPGRLGFEITETAGLGNFKEV
                     RNWMRRMKALGCSFAIDDFGSGHTSMSYLSDLPVDIVKITGTIVTSMVGNPSHGLVVE
                     AINSVVHVVGAWTVAESVENEEALLELKNMGIDFAQGFYFAPPTDLADIPESILAQLG
                     FRIVR"
     gene            complement(2658..2733)
                     /locus_tag="PLU15_10805"
     tRNA            complement(2658..2733)
                     /locus_tag="PLU15_10805"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2697..2699),aa:Glu,seq:ctc)
     gene            complement(2740..2813)
                     /locus_tag="PLU15_10810"
     tRNA            complement(2740..2813)
                     /locus_tag="PLU15_10810"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2779..2781),aa:Gln,seq:ctg)
BASE COUNT          825 a          884 c          798 g          761 t
ORIGIN      
        1 gcgcaagagc gaccacgcgg actggtcggt ctacgctcag tactttcctc tctttcgacc
       61 catgtccttc cataactgca aataccaccc ggatgatatt tgagccatac cttggaggtt
      121 tttcaaacag ttcgtcgaaa gttaacgtgg aataacacgc catttctctg ctccaacgtt
      181 cgctggcaca atagcttact cttgaggggg cttagccctt gatcttgtca cgatcgcgaa
      241 aaccctttgg caatataagc tcatcatcgc cggagtcttc caccgactgg gtccagtgca
      301 ctttttgggc aatgttattg tcctcaatca tcctgtcaac cgtcattgtc agagcgaatc
      361 atctgtgtcg tgaagtcaaa aacaaccttc tatctcgtgc aacaaccgct gccgccttga
      421 tagatcctgc cattctttcc aatctggaag aagcgtttag tcttcttctc cggccgtgct
      481 ccaacagggt tgcatccccc aaccaaacac gcccaagcct caccgctgca tacatcgtca
      541 gcctagacag ccagagaatc aggttcatta gcggcgcatc cgctcaaccg ggttccagca
      601 atactctgcc taatcccggc gatgtaattg gcagagcatc tccggaagag gtggctctga
      661 tttttgccgg gcatccaatt gtcagaggcc cgtaccccag tggtcgcagc aaatcggtca
      721 cagcgtatgc cccggtaatt gacactcgca caggcaccgt ggttgctgct ctggccctcg
      781 aagttgacgc ggcagactgc ccagagccac ttgcaaaggg atggtcggtg atcgccttcg
      841 cgctagccct tacaggaaca ctcggcatag cgctgccacg aatgcacgct gagaacgagg
      901 cgaccgaaca actccgcacc gccagccatg accagctcac aggcttggcc aatcgttctg
      961 cccttatcag cgaaattgaa cgggctatcc gggaggcagg aaccgaccta cagagcaccc
     1021 tcctactcat cgacatagat gacttcagga cattgaacga cactctttct tacggagtag
     1081 gcgacgatgt actagtgcaa gtagcacaac acataagaga cgaggtgcga agggaagatt
     1141 tcgttgcgcg cttcggcgaa gacgctttcg ccgtacttgt ccgcacaccc gccattgaac
     1201 aggcagtggt taccgcagag cgcatacgag catctttgga gcatgtggaa gtggagaccg
     1261 gaggcaccgc aattacagtc tacttgacag taagcgtcgg aatagcggaa atagacgcga
     1321 actcatccgt caaagacgtg gcgcttcgcg ccgattcagc cctctgcgca gctaaatcag
     1381 cgggaaaaaa ccgaattgta tgctccagcg tcgccgctgg aggcagggac gaacccgacg
     1441 catacacgca gatagcgatg atagaggatg ccttagaaca tgataaattc atactccatt
     1501 tccagcccat tgttcctata gtcccgatca accccaaccg tggtagcaaa aaccatttcg
     1561 ctgaccaaga ccatgccctc aagtgggtgg gctatatgga tgggtctcgc ctccatgaag
     1621 ctctcgtgcg catagagggt cccaacggtg agatcatccc acccgggaaa ttcatccctt
     1681 gggcagaggc aatcgggtta gtagcacaaa tagacctatg ggtgctggag catgcgatag
     1741 aggtacttaa tgagaggccc gacatgaatg tggcgataaa cctatcagca agaagcatca
     1801 gcaggccatc atttttgacc caagtggaac agcgcgtagc aagcagtgga ctcgcgccgg
     1861 gaaggcttgg atttgagata actgaaacag ccggtctggg aaattttaaa gaagtgcgaa
     1921 actggatgcg gcgcatgaag gcgctgggtt gctcttttgc tatagatgat ttcggatcag
     1981 gccacacatc catgtcgtac ctgagcgatc ttccagttga cattgtaaag ataacgggaa
     2041 caattgtaac atcaatggtg ggaaatcctt cacacgggtt ggttgtagag gctatcaact
     2101 ctgttgtcca cgtagttggc gcttggacag tggccgaatc tgtggaaaac gaagaggcat
     2161 tgttggaact gaaaaacatg gggatagact tcgcacaagg tttctatttc gcacccccaa
     2221 cagatctggc agatatcccc gaatccatcc ttgcgcaact aggcttccgc atagtccggt
     2281 agattgactt gctcaagtgc cgcacaacag cataagtctg cctgctcatg aaccctgatg
     2341 cagaaagcca ccatcaacct tcatttcgtc caacaaaccg atgacatggt cagccaagcc
     2401 gcctacaaat cttgcaagat gaatacgagt gtttgcgcgt gtcttgccca cgctgatcga
     2461 atttcttcta ctctacagga aatgacatga tagcaaggtt tttattcgga gaaacgtgtg
     2521 gttcgcaggc atgtcttccg agaccctcaa gcaggttcat gccgtatttg gccgtccacg
     2581 cattgaatat tgaatgcgaa attcataata aagtacacaa aagaaaacgc cctgacaact
     2641 cagggcgaat actcttctgg tagccccgac ggagttcgaa tccgtgtctt cgccttgaga
     2701 gggcgacgtc ctagaccgct agactacggg gccattaatt ggctggggag gaaggattcg
     2761 aaccctcgca atcagatcca gagtctgacg tcctaccgct agacgactcc ccagtggaat
     2821 cagtgtccat tgccgattat agcagaggct ccgacgtttg taaagtgttc gagcaactcg
     2881 caaaacgaaa atacaggatt tcgcatatcc gtttcggcct ggccatatat tatgctgcta
     2941 cggtccccta tcccccattt gctctgcttt ggcggatgga ctctcccgca ccaggtggta
     3001 gctctctcgt ttcaccttca taacaaccta tttgttccaa gcgttcacgg catttgtaaa
     3061 tggagtctgg acatacagag catcgtcgaa caagatgtcg cagtatctgt agttctgatg
     3121 ttcagccctc atctaagtat cagttgatca tgtcgaaaag cctatacacc cggattgcat
     3181 agtccacaaa ctgtttcagc ctattcatct cctccgcatc ttggctttca cttagcgtgt
     3241 ggaatgtcga atagcttcgg cctcagtt
//
LOCUS       DAOSYL010000119         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_23451_length_3242_cov_10.883590, whole genome shotgun
            sequence.
ACCESSION   DAOSYL010000119 DAOSYL010000000
VERSION     DAOSYL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23451_length_3242_cov_10.883590"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1402
                     /locus_tag="PLU15_10815"
     CDS             200..1402
                     /locus_tag="PLU15_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31431.1"
                     /translation="MDMILQPEGPIRLGDFLLSHLSDSGWTEFRAGVAFVKYSGVRHI
                     AAPLADFAKRGRVQLCVGVDFGGTTAEGLKALLDCAGVNGEIWVYHNEAGVTFHPKLY
                     FFKAKDGRADVAIGSGNLTEGGIYTNYEVAMLLRLVPSSPDHRKLITQFESTLNAWCD
                     PSTGMAKRVDIALIEKLLDQGYIVTERQAARAQAAKASPARTKPGKWMPIFSARPVGK
                     APAAVMWPGPTPFREPSGTGQPPAVQPPQAVAATGFLMTLQQTDIGVGQVTAGTSRRS
                     PEVFIPLAARDYAPDFWGWPSLFAPDPAKLGKMDRPGVKMWLGTKVIEVNMMTWPDKH
                     DFRLRSEALRSAGSIGDILRLEKADATIGFDYLAHIVPQGTQEHRHYLAFCVNRTKGR
                     SNKLWDYY"
     gene            complement(1396..3003)
                     /locus_tag="PLU15_10820"
     CDS             complement(1396..3003)
                     /locus_tag="PLU15_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31432.1"
                     /translation="MSKKIICRMKPYIQPFERHLAVAELSALSGVGLSLDDAQGTNFE
                     ITTETSGQTLASLLSYYEYVQDGAKGFVTSQVLREATLYFARNGISLQDIRKQFPAKG
                     DVTMPNRRCLRYGTHGIHEYRGKFFPQLVRSLINIAQVPSSGIVADPMCGSGTTLVEG
                     VLAGRQTIGLDMNPLSVFMSRVKCEMLRVKPESLEEHYNRVRDELLQLRPRSVAKRLE
                     YFRTLEQKDQDYLKRWFAPQVLEDLDVIAGHIAVVEETNLRDFMRLVLSNIIRRVSWQ
                     KEDDLRIRKEIRTDIEIDPIREFLEELGRSVRIVMGFLCQIRGTRLGDAQVAEGDAKQ
                     MCKHWHDLAGKVDAVITSPPYATALPYLDTDRLSLLYLGLLPREAHRGRDKEMIGNRE
                     ISEGIRREYLERFRKEGKLLPKSVTRLIGKIDQLNAGGSVGFRRRNLPALLAKYFLDM
                     RTVLAGIAHILRPEAPAFVVVGDNHTIAGGQRVDIETAGLLAEIARTVGLEEGNHLPM
                     EMLVSRDIFRRNAVASETILEFRKPGQ"
     gene            complement(3006..>3242)
                     /locus_tag="PLU15_10825"
     CDS             complement(3006..>3242)
                     /locus_tag="PLU15_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOO31433.1"
                     /translation="LSLHLRAGRYAAFVIGDSIIDGKLVSNADILAEVADSFGFREVA
                     RINRNIQATKKAFNPKIGKIKQEQILILQNRKVY"
BASE COUNT          767 a          919 c          809 g          747 t
ORIGIN      
        1 gtgtttcatg ggcgggacgc ccatgtcacg agggggtttt gcaagagcct cagtttgaat
       61 tatagctggt catggccgcc tttgccatca cgaatctgac acacacccac aataaaatgc
      121 aaatcaaaaa agggttgcaa acctccatag gattggatac aaagcgccca ataagggaga
      181 ctactaggag gcaaagcaca tggatatgat attgcaacca gaaggcccca ttcgcttggg
      241 tgatttcctt ctaagccatc tctcggattc gggttggacg gaattccgtg ccggagtggc
      301 ctttgtgaag tattccgggg tccgccatat tgccgccccg cttgccgatt tcgcaaaacg
      361 tgggcgtgtc cagttgtgcg ttggtgtgga cttcggtggc actacagcgg aaggtctaaa
      421 agctctcctt gattgcgcag gggtaaacgg agagatatgg gtttatcaca atgaagccgg
      481 tgttacattt cacccgaaac tgtatttctt caaggcgaaa gatgggcgtg cggacgtagc
      541 catcggatcg ggtaacctca ccgaaggcgg catctacacg aactatgagg tggcgatgtt
      601 gctgagactc gtcccctcgt caccagatca ccgcaagctg attacgcagt tcgagagcac
      661 acttaacgca tggtgcgatc cgtcaaccgg catggcgaag cgagtggaca ttgcgttgat
      721 cgagaagctc cttgaccaag gttacatcgt cacggagcgc caagctgcca gagcccaagc
      781 tgcgaaggca tcaccagcac ggacgaagcc aggtaaatgg atgcccattt tctccgcgag
      841 gccagtggga aaggctcctg ccgccgtgat gtggcccggc cccacgccgt tccgtgaacc
      901 ttccggcacc ggacagccgc cagcagtcca gcctccgcaa gctgttgcag cgaccggatt
      961 cctgatgact ctacagcaga ccgatatcgg cgttggtcaa gtcaccgctg gaacatcgcg
     1021 ccggtccccc gaggtgttca ttccgcttgc agcacgcgat tatgcccccg acttctgggg
     1081 ctggccgtct ctatttgccc cagacccggc caagctaggc aagatggacc ggccgggggt
     1141 caagatgtgg cttggaacga aggtcatcga agtcaacatg atgacttggc cggataagca
     1201 cgacttccgc ctgcgaagtg aagccctgcg aagcgccgga agcattgggg atatcttgcg
     1261 tcttgagaag gcggatgcaa ccattggctt cgactaccta gcccacatag tgccccaagg
     1321 aacgcaagaa caccgccact acctcgcctt ctgtgtgaac aggaccaagg gcaggtcgaa
     1381 caagctctgg gactactact gacctggctt cctgaattcg aggatggttt ccgaggcgac
     1441 ggcgttacgt cggaagatgt cacgcgacac cagcatctcc attggcaggt gattcccctc
     1501 ttcaagcccc accgtccggg caatctctgc cagaagcccc gcagtctcaa tatccacccg
     1561 ttggccaccg gcaattgtat gattgtcccc cacaaccacg aaggctggcg cctctggtcg
     1621 aagaatgtgc gcaatgccag caaggaccgt cctcatatcg aggaagtact ttgcgagtag
     1681 tgccggcaga ttcctacggc ggaagccaac actgcctcct gcgttaagct ggtctatctt
     1741 gccgatgagt cgcgtaacag acttggggag cagcttccct tccttgcgga acctttcaag
     1801 gtactctcgt cgaatccctt cgctgatctc gcgattaccg atcatctcct tgtcgcgacc
     1861 acgatgtgct tctcgtggta gcagtccgag atacaacaag ctcaatctgt cagtatcgag
     1921 atacggtaaa gcggtagcgt acggcggcga cgttattaca gcgtcaactt ttccggcaag
     1981 gtcatgccaa tgtttacaca tctgtttggc atctccttcc gcaacttgcg catcccctaa
     2041 gcgcgttcct cgaatctggc aaagaaaccc catcactatc cgcacagaac gcccaagttc
     2101 ttctaggaac tctcggattg gatcgatctc tatgtcggtt ctgatttctt tcctgatcct
     2161 caaatcatcc tctttctgcc atgacacccg ccggataata ttactcagca ccaatcgcat
     2221 gaagtcgcgt aagtttgtct cctcaacaac cgctatatga ccggctatca cgtccagatc
     2281 ttctagaact tgaggagcga accaccgctt gagatagtct tggtccttct gttcgagcgt
     2341 ccgaaagtat tccagtcgct ttgcgaccga ccgtggccgt aattgcaaca attcatcgcg
     2401 tactctgtta tagtgctctt ccagcgattc gggcttgacc cgcagcatct cgcacttaac
     2461 acgtgacata aacactgaca ggggattcat gtcgagaccg atggtttgac gccccgcaag
     2521 gactccttct acaagcgtag taccgctacc acacattgga tcggccacga tgccactgga
     2581 aggaacctgc gctatgttga ttagtgatcg aaccaactgt ggaaaaaact taccacggta
     2641 ctcatgaatc ccatgcgttc catatcgaag gcaacgccta ttcggcattg taacatcccc
     2701 ttttgccgga aactgcttcc tgatgtcctg caaagagata ccgttacggg caaagtaaag
     2761 cgttgcttcc cgcaacacct gactagtgac aaacccttta gcaccatcct gaacatattc
     2821 atagtaggac agtaggctgg caagtgtctg gcctgaagtc tctgtcgtga tttcaaagtt
     2881 ggtgccctga gcgtcatcca acgacaaacc gactccagac aaagcgctca actcagccac
     2941 agccaagtgt cgctcaaaag gttgaatata cggcttcatc cggcaaatta tcttcttact
     3001 cattttcagt aaaccttcct gttctggagt attaatatct gctcctgctt aatcttaccg
     3061 atcttcgggt tgaaagcctt ttttgtcgcc tggatatttc gatttatccg ggctacttcg
     3121 cggaagccaa aactatcggc gacctctgca aggatgtcag cattgctcac aagcttgccg
     3181 tcaatgatcg aatcgccgat cacaaaagcg gcatatcgtc ccgctcgaag atgaagagac
     3241 aa
//
LOCUS       DAOSYL010000120         3213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_23709_length_3213_cov_5.740025, whole genome shotgun sequence.
ACCESSION   DAOSYL010000120 DAOSYL010000000
VERSION     DAOSYL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23709_length_3213_cov_5.740025"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1110..1304
                     /locus_tag="PLU15_10830"
     CDS             1110..1304
                     /locus_tag="PLU15_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31434.1"
                     /translation="MNWLKASVAGTIGTGTITLSYGADGRKAADTSDFHAGKPWAYLR
                     NYPGNYMDWQLFTVSVKVPF"
     gene            complement(1415..3178)
                     /locus_tag="PLU15_10835"
     CDS             complement(1415..3178)
                     /locus_tag="PLU15_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31435.1"
                     /translation="MRHITRLVVISREYWRWMVLALIAMLGVTAASLTGPWLIRSLIG
                     TIEEGFGHGTTATSQVINISAILLLVYALRPALRALQTWATHVAGWGSVAAARKEIYE
                     HLQRLSPKYYSHTQTGQIMSRAINDTAYFESLIAHVIPEAIVSLFTVAGVFVVLFSIN
                     ATLALYTLAPIPLIMLGFIAYNRYVRPLFRHAQAKLGDLNAVLQDNLSGMREIQVFTQ
                     EEREMESVGRRIMVHSSAITRAVSTSACFHGAIDFFAGLGTVSVVLFGGLMAAKAQIS
                     IADITGFLLYVSLFYEPIMGLNRLNESLQQALAASDRYFEVLDIRPDIGDSPDAVQLG
                     KARGLITYEDVSFNYDDTPVLKNINLEIKPGEMVALVGPTGVGKTTMANLIPRFYDPA
                     EGRVLMDGVDIRTVAISSLRRNVGMVLQDVFLFNGTVAENIAYGSPDANLEQIIDAAV
                     AAGADEFIREMPRGYDTHIGERGVRLSGGQKQRLAIARALLYDAPILILDEATSSVDT
                     ETEAKIAAALEKLIGGRTTIVIAHRLSTIRHADRIIVLDEGEIVEQGTHEELMRLNGL
                     YSKLVTVDSDASSSYTAIYRP"
BASE COUNT          699 a          878 c          931 g          705 t
ORIGIN      
        1 cagcactacg gaatagcttc ttagctcttc tttcctactg tagattgacg cggtcgttga
       61 acaggcagct ctgactctgt acaacaccga tcccaaactc gctgcaagct tcctggctaa
      121 ctactccaac gctcatgctg agcgagtagt acaggcctgg tgggagcttg ccgacaagct
      181 catggttaag taccaggatg gttacgtaaa tctgaagaca gtaggctacc ctgaggagtg
      241 gctgaagtca ttcggattcg ggccgatcgt aaggccttaa cacgtagttg ctgagctgca
      301 gcactggcgg cagctggaca tcggcacggg cgagcgtcgg gctcgcccgt gtctttgtca
      361 tgtgaccatg gccagccagg tgcatgtagg gattgtggct tttgaggtag cagagccccc
      421 gcattcaggc gtgtggtttc gccagggcga cagcggaagc ggttgggggc gcaccggggc
      481 cagggatagg cccaggggta ggctcaggga tatacccccg ggggtatccg ccccgagata
      541 cgatcacttt ttaacaatac tttcggcgaa aaacgcaact tctgagtaca tcgatttggg
      601 ccgagtgccc cggggggcta atcgaccccg caaaaggagc cctcggggcc ggataccgcc
      661 gatttagtgg tgaatcgatg gcactacgcg ccatcacctg tgcaccgtta ccaaatgaag
      721 ccatgatgtg ggatattatg tcggtccgtc agcctgcctg gctgccccaa cccgtcggat
      781 accccccgat tttcgtgatt tggaggtggt caggtggccc ttcgaaatcg atgtactcaa
      841 aagttgcggg tatctccaaa actaatggca aaaatcgacg atatgccagc tccagccgtt
      901 ttgtccgacg gccatgatgc caagaccagc taaaccgcca ttccagcgaa gcgagcatga
      961 ccccctcagc caacaatcca ccgtgatgat gggggaggat gtcattttcc ggcttactat
     1021 cccgcctggc gatgtctacc cgctttttca actgctattg cccacaacaa ccacggggag
     1081 gctgaacttt tggttttgac gacgggggca tgaactggct gaaggcctcc gtagccggaa
     1141 ccatcgggac aggtacgata accttgagct acggagcaga cgggcgcaaa gcggctgata
     1201 caagcgattt ccatgcaggc aagccctggg cctacctgag gaactaccca ggcaactaca
     1261 tggattggca gctgttcaca gtaagcgtca aggtgccttt ctaggcgctt gatgcagaag
     1321 gccccccggc gcgttccggg gggccgttcc tatttggtac agccgaagac gttcgcagtg
     1381 acgacgatcg tcgagtgtaa gttcgccgaa gccttcacgg gcgatagatc gcggtgtagg
     1441 agctcgatgc gtctgaatca accgtaacca gtttggaata gaggccattc agcctcatca
     1501 actcctcatg tgtcccttgt tcgactatct cgccttcatc caggacaatg atcctgtcgg
     1561 catggcgaat agttgagagg cggtgggcga ttactatcgt cgtcctgcct ccaataagct
     1621 tttcgagtgc agccgcgatc tttgcctctg tttccgtgtc taccgatgag gtggcctcgt
     1681 ccagaatgag aattggcgcg tcgtagagaa gggcccgtgc tatggcaagc ctctgtttct
     1741 gcccacctga gagccttacg ccgcgctctc ctatgtgggt atcgtaccca cgcggcatct
     1801 ctctgatgaa ctcatcagcc cctgccgcga ctgcggcatc gatgatctgt tccaggttag
     1861 cgtctgggct gccgtatgcg atgttctcgg cgactgtgcc gttgaacaag aacacgtcct
     1921 gcaaaaccat gcctacattc cgacggaggg aagagatcgc aacagtgcgg atgtctaccc
     1981 cgtccatgag aacacgtcct tcggcgggat cgtagaacct ggggatcaag ttggccattg
     2041 tcgtctttcc aacaccagtc ggacccacga gggcaaccat ctcgccgggt ttgatttcca
     2101 ggttgatgtt cttgagcacg ggggtatcgt catagttaaa ggagacatcc tcgtaggtta
     2161 tgagaccccg agccttgcca agctggacag cgtcgggtga atcgcctatg tcgggccgga
     2221 tgtcgagcac ttcgaaatag cggtcggagg cggccagagc ctgctgaagg gattcattga
     2281 gacggttgag ccccatgatg ggctcgtaga agaggctcac atagagaaga aacccggtga
     2341 tatcggcgat ggatatctgt gcttttgccg ccatcaagcc tccgaacagg acaactgaaa
     2401 ctgttccgag ccctgcgaag aagtcgatcg cgccgtggaa acaggcgctt gttgacacag
     2461 ccctcgtgat agccgaagaa tgcaccatga tacgcctacc caccgactcc atctctcgct
     2521 cttcctgcgt gaacacctgg atctcccgca ttccgctgag attatcctgc aagacggcat
     2581 tcaggtcgcc cagtttcgcc tgggcatggc ggaagagggg gcggacgtat cggttgtagg
     2641 cgatgaaacc aagcatgatc agcgggatgg gcgccagggt gtatagagcc agcgtcgcgt
     2701 tgattgagaa gagcacgacg aagactccgg cgactgtaaa cagactcacg atggcttcag
     2761 ggatcacgtg ggcgatcaag gactcaaaat aggcagtatc attgatggcc ctggacatga
     2821 tctgtcctgt ctgggtgtgg gagtagtact ttggcgacag tctttggaga tgctcataga
     2881 tctccttccg agctgcagcc acactgcccc atccggcaac atgggtagcc caggtctgca
     2941 gggctcgaag cgcgggcctg agggcataca cgaggagcag aatggcggat atgttgatga
     3001 cctggcttgt cgcggtcgtc ccgtggccga agccttcctc aattgtgccg ataaggcttc
     3061 ttatgagcca ggggcctgtg agacttgcgg cagtcacccc cagcatggca atgagtgcta
     3121 ataccatcca acgccagtat tcacgcgata ttactacaag acgggttatg tgtcgcaacg
     3181 cctggtcacc tcatgttgag tgttcgccct ttt
//
LOCUS       DAOSYL010000121         3210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_23753_length_3210_cov_4.955626, whole genome shotgun sequence.
ACCESSION   DAOSYL010000121 DAOSYL010000000
VERSION     DAOSYL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23753_length_3210_cov_4.955626"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLU15_10840"
     CDS             <1..629
                     /locus_tag="PLU15_10840"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO31436.1"
                     /translation="DTMSYTDLIEMANQGAKVVHLRAVEIAMHSRIPLAIKSTFSDSP
                     GTLVADVARSAVATFAPPRVVSAVTTVAERTQLVLRGDSYSDTGSVLGVFATLADQRI
                     SVDMINVFPDRIAFIVASREATAAIATLKELGIEAETNPGCAKVSVVGEGMRGVPGVM
                     ARVARALHDASVRVLQTSDSHYSISCLVPEPDMERAARALHAQFELGK"
     gene            657..1574
                     /gene="dapA"
                     /locus_tag="PLU15_10845"
     CDS             657..1574
                     /gene="dapA"
                     /locus_tag="PLU15_10845"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOO31437.1"
                     /translation="MNALTKDGKLARVLTAMVTPFAEDLSVDYRKARALARRLVQNGS
                     DGLVVSGTTGESPTLSHEEKLKLFEAVLDEVGNEAVVFAGTGTNDTRASIEMTRDAER
                     VGVQGAMLVTPYYNKPPQAGLYRHFAEIAESTGLPLLVYNVPGRTSVNLAADTMARLA
                     RDFDNIIAVKEASGVLEQVAEIRSKAPEHFIIYSGDDNMTIPIMAVGGDGIVSVASHV
                     AGPAMQEMIAAFVDGDIARAGALHRRLLPLFKVIFVTTNPIPVKAALALAGFDAGPLR
                     PPLIEATEAERAQIRRVMESLGLIANEST"
     gene            1692..2642
                     /locus_tag="PLU15_10850"
     CDS             1692..2642
                     /locus_tag="PLU15_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOO31438.1"
                     /translation="MSPNMNSRSRSKKTISIIIIAIMSLLLVILAVVPHIIMSPVLGK
                     RVERPQYISEDYGITAEQISLKTEDGLSLAAWHTKANSGRGTIILLSGIESPSVTAFF
                     GYAKMFADNGWDSLLIEMRGRNLSEGREIGLGYTEWNDVVAGVNYLSDNQEASDLPII
                     AMGTSLGGATSIMAAGKDPRVDGVIAISAFSSWEDAFSDNMNLVNVPRLFCTLEKPFV
                     KLYLGIHYGFDTVSYSPLKALEDFGNRPLLLMHSTKDSQVPYPSFERLRKQAEKCNID
                     TSTFVREGDEHFLCYEEYFDNPLQDTEFSDSILRFLNANY"
     gene            2954..>3210
                     /locus_tag="PLU15_10855"
     CDS             2954..>3210
                     /locus_tag="PLU15_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO31439.1"
                     /translation="MLVVECGSDIVVVDSGLMFPDEEMFGVDLVIPDVSYLEENRERI
                     RGIVLTHGHEDHIGALPYVLKRLNCNIPVYGTRLTLALVES"
BASE COUNT          750 a          821 c          921 g          718 t
ORIGIN      
        1 aggacaccat gtcatacacc gacctcatag agatggccaa ccagggcgcc aaagttgtcc
       61 acctgcgggc agtagaaatc gccatgcact ctcgaattcc gctggctatt aagagcacat
      121 tttcagattc ccccggcacc ctggtggcgg atgtggcgcg tagtgcagtg gcgaccttcg
      181 ccccgccccg cgtggtctcg gcggtgacca cagtcgccga acggacgcag ctggtgctac
      241 gcggcgattc atatagcgac accggatcgg tgctgggggt gttcgctact ctggccgatc
      301 aacgcatttc ggtggacatg atcaacgttt tccctgatag aatcgcattc atcgtggcgt
      361 ccagggaggc gaccgcggcc atcgcgaccc tgaaggaact gggaattgag gccgagacca
      421 atccgggttg cgccaaagta tcggtagttg gtgagggcat gcgcggcgtt cccggtgtca
      481 tggccagggt agcgcgggcc ctgcacgatg cgtccgtccg cgtgctgcag acctcagact
      541 cgcactactc aatatcgtgc ctcgtgcctg aacccgacat ggaacgcgcc gcacgcgcgc
      601 tacatgccca gtttgagctg gggaaatagg tgcgcagagc ataggaggtg aagatggtga
      661 acgcacttac aaaagacggc aaactagccc gcgtgcttac ggcaatggtg accccgtttg
      721 ccgaggacct atctgtcgac tacaggaagg cccgggcttt ggcgcggcgg ctcgtacaga
      781 atggatcaga tgggctcgtg gtgtcgggca cgacaggcga atcgcccacc ctcagccacg
      841 aggagaaact caagctgttc gaggccgtgc tggacgaggt gggcaacgag gcagtggtct
      901 tcgccggcac gggcactaac gacacgcgag caagcatcga gatgacacgt gacgccgagc
      961 gcgtgggagt gcaaggcgcc atgctcgtga cgccgtacta caacaaaccg ccgcaggccg
     1021 ggctgtaccg tcatttcgcc gagatcgccg agtcgacagg cttgcccctg ctggtctaca
     1081 acgtgcctgg gcgtacgtcg gtgaacctgg cggccgatac catggctcgc ctcgctcggg
     1141 atttcgacaa tataatagcc gttaaggaag catcaggggt gctggagcag gtggccgaga
     1201 ttcgctccaa ggctcccgag cacttcataa tctatagcgg cgatgacaac atgactattc
     1261 ccatcatggc agtgggcgga gacggcatcg tcagcgtggc atcgcatgtt gccggccccg
     1321 cgatgcagga gatgatcgcc gcgttcgtgg acggtgacat tgcccgtgca ggcgctttgc
     1381 atagacgcct gctgcctctg ttcaaggtga ttttcgtaac cactaacccc atcccggtaa
     1441 aggctgcgct tgccctcgcc gggtttgacg cgggccccct caggcctccg cttatcgaag
     1501 cgaccgaggc cgagcgggcg cagatccgta gggtcatgga gtcgctgggg ttgatcgcga
     1561 atgaatcgac ctgagcgtaa aggaagagtg gaggaaaggg aagccgccag gtttggcggc
     1621 ttccgaggct aaaacaacag cttatggtat acttgtgcat atgtgaatca tcgtagagag
     1681 gatggtcata tatgagcccg aacatgaata gcagatcaag gtcgaagaag actatttcaa
     1741 tcatcatcat tgctattatg tcactattgc tagtcatttt ggcagtagta ccacatatca
     1801 ttatgagccc tgtcttgggt aaacgagtgg aacgtccgca gtacatatct gaagactatg
     1861 gtattaccgc tgaacagatt tctctcaaaa ccgaagatgg tctttcactt gcagcatggc
     1921 acaccaaagc taatagcggt aggggaacta tcatactact atctggcata gagagtccat
     1981 cagtaacagc tttctttgga tatgcaaaga tgtttgcaga caatgggtgg gattccttgc
     2041 taattgagat gcgtggaaga aaccttagcg aaggcagaga aatcggtcta ggttatacag
     2101 agtggaatga tgtagttgcc ggagtaaact atctatcaga caatcaagaa gcatctgacc
     2161 ttcctatcat cgctatgggg acttccttgg gaggggctac tagcattatg gcagctggca
     2221 aagatcctcg tgttgatgga gtcattgcta tctcagcttt ctcaagctgg gaagatgcct
     2281 tttcagacaa catgaactta gtgaatgttc ctaggttatt ctgtacactg gagaagccat
     2341 ttgtaaagct atatttagga attcactatg gattcgatac tgtcagctat tctccattga
     2401 aggcgcttga agactttggt aacagacctt tgctgttgat gcattcgacc aaagactctc
     2461 aagtgcccta tcctagtttt gagcgtttgc gaaaacaggc cgaaaagtgc aacatcgata
     2521 cttctacatt tgttcgcgaa ggggatgaac actttctttg ctacgaagag tactttgaca
     2581 atccactaca agacaccgag ttcagtgatt ccattttacg cttcttaaat gccaattact
     2641 gatatcgata accaaagagc cgagagcggt cggacagaat tctgtccggg gcaggcgagg
     2701 aacaatccgt acttcgcacg cggcctgcag tgtccacccc tcgaccgcgc gaaacgcttg
     2761 gccaaatcct cgggtcctct gcagtaagcc ctcgggtggg gtaagccttt acgttggtcg
     2821 gatccgcggt gtataatctt aggagtctga tcaaaatccc atttgcgagg tgcaattttc
     2881 gatagtgaca tcgacaaaga atgattctgt tcgcctcatc ccgctcggcg ggatagggga
     2941 aatcggcaag aacatgctcg tggttgaatg tggctctgac atcgtcgttg tcgattcggg
     3001 gctcatgttc ccggatgaag agatgttcgg cgtagatctg gtcatacccg atgtgtcgta
     3061 ccttgaggag aaccgcgaac gtatccgcgg gatcgtgctc acgcatgggc acgaagatca
     3121 catcggagcg cttccatatg tgctgaagcg gctgaactgc aacataccgg tctacggcac
     3181 acggctgacg ttggcgcttg tggagtcgag
//
LOCUS       DAOSYL010000122         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_24037_length_3177_cov_7.800448, whole genome shotgun sequence.
ACCESSION   DAOSYL010000122 DAOSYL010000000
VERSION     DAOSYL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24037_length_3177_cov_7.800448"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLU15_10860"
     CDS             complement(<1..320)
                     /locus_tag="PLU15_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31440.1"
                     /translation="MADTEEKSTSRITGFFSAAYKDFGLPRLIITGFVLFLFILATIM
                     KMDLTILISDSLVRIGMNGVLVLSMLPTLACGVGLNFGLPVGVIAGLIGAVMSMNWNL
                     QGFAG"
     gene            complement(322..1932)
                     /locus_tag="PLU15_10865"
     CDS             complement(322..1932)
                     /locus_tag="PLU15_10865"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO31441.1"
                     /translation="MSITEGNVLELEGISKSFFGTQVLKDVNLSVRPGEIHALVGENG
                     AGKTTLMNILFGMQVIFDTGGFEGRIILDGVPTDISSPREAMTYGIGMVHQEFMLIPG
                     FTVAENIKLNREPTLPNLASKIFGQKLETVDEEAMRADARAALDKLAVSIDELALVAG
                     LPVGHMQFVEIAREIDKHSVELLVFDEPTAVLTESEAQMLLDAMKRLASEGIAILFIT
                     HRLDEVMEVADNITILRDGEVVAMMPKAEAEVERIAELMVGRKVERIELPERAKEPSD
                     RDIVLRIENLSVNMPGERVRNFSLNVRRGEILGIGGLAGHGKVGIANGVMGLYPSTGK
                     VYANEKELRLNDPKSALEAGMAFVSEDRRGVGLLLDESIEMNIALTAMQVQEKFLRPG
                     AIPSLRLADEAAIREHALQVIKDLDIRCNGPEQLVRRLSGGNQQKICLGRAFTMQPEV
                     MWVSEPTRGIDVGAKQLVLDLLVDFNRKHGMTIIMTSSELGELRKVCDRIVIVYRGQI
                     AGVLPPDASDVDFGLMMAGKGAARREVG"
     gene            complement(2031..3146)
                     /locus_tag="PLU15_10870"
     CDS             complement(2031..3146)
                     /locus_tag="PLU15_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOO31442.1"
                     /translation="MAKRSVLAAVALALILGLASVSIGAADFKIGVVTGTVSQGEDEY
                     RGAEAAIQKYPGLILHRSYPDNFMQEQETTIAQITGLASDRQVKAIVINQAVPGTVAA
                     IRKVREIRPDIIFVLGEPHEDPPIVEKYADVSLIPNNIARGTTIVRLAKKLGAKTFVH
                     YSFPRHMSYADLATRRDIMEEECKKLGMRFVFANAPDPMGEGGLPAAQQFILEDVPRK
                     VGQYGKDTAFFSTNCGMQEPLITAVIKTGAIFPEQCCPSPTHGYPGALGLEITDNMKG
                     NFPAIIRAINAEIVKKGQAGRMATWPVATGYLNTIAGVEIAKLAVEKKLNYKDMDAVR
                     KVVQGIAGTPIEFERLSAGGQFYRYLIDSIIFGVTVK"
BASE COUNT          684 a          980 c          759 g          754 t
ORIGIN      
        1 ccggcaaacc cttgaaggtt ccagttcatg ctcattaccg ccccaattag tcccgctatt
       61 actcccacag gcagtccgaa gttgagccca actccacatg ctagcgtggg caacatcgag
      121 aggacaagca ctccgttcat gcctatacgt acaagggagt cgctgataag gatagtcagg
      181 tccatcttca tgatagtagc caggatgaac aggaacaaca cgaaccccgt gattatgagg
      241 cgaggaagtc caaaatcctt ataggcagcg gaaaaaaacc ccgttatgcg actcgtggat
      301 ttctcctccg tatcagccat gtcaaccaac ctcccttcgc gcagcaccct tgccagccat
      361 cataaggccg aagtccacgt ccgaagcatc cggcgggagg acacctgcaa tttgcccgcg
      421 atatactatc acgatccggt cacatacctt tcttaactcc ccaagctcag acgatgtcat
      481 gatgattgtc atcccatgct tgcggttgaa atccacgagc agatcgagaa cgagctgttt
      541 cgcgcctaca tcgattcccc tagtcggttc ggagacccac attacctcag gctgcatggt
      601 aaatgccctt cctagacaga tcttctgctg gttccccccc gacagccttc tcacaagctg
      661 ttcgggtcca ttgcacctga tatcgagatc ttttatgacc tgaagcgcgt gttcgcgtat
      721 cgcagcttca tctgccagcc tgagcgaggg gattgccccc ggtctcaaga acttctcctg
      781 cacctgcatt gccgtgagag cgatattcat ctcgatagac tcatccagga gcaatccgac
      841 gccgcgcctg tcctcggaaa cgaaggccat ccccgcctca agcgcactct ttgggtcgtt
      901 taaccgcagc tctttctcgt tggcatatac tttgcctgtc gacggataga gccccatcac
      961 tccgttcgcg atccccacct tgccatggcc tgcaaggcca cctatgccca atatctcacc
     1021 gcgcctcacg ttcagactga agttgcgcac gcgttctccg ggcatgttga cgctcaagtt
     1081 ttcgattctg aggacaatgt cgcgatcaga tggctccttc gccctttcgg gaagctctat
     1141 gcgttccact tttctgccga ccatgagctc ggctatcctc tctacttccg cctcagcctt
     1201 tggcatcatc gccacaactt cgccatcacg gaggatagtg atgttgtcag ccacctccat
     1261 gacctcgtcc agccggtgag tgatgaaaag tatggcaatt ccctcagacg caagcctctt
     1321 catggcatcg aggagcatct gcgcctcgct ctctgtgagg actgccgtgg gttcgtcaaa
     1381 gacaagaagc tcaacgctgt gcttgtcgat ctcccgcgct atctcaacaa actgcatgtg
     1441 tcccacaggc agacctgcca caagcgccaa ctcatcgatg gagactgcca gcttgtcgag
     1501 ggccgccctg gcatcggctc gcatagcttc ctcgtcaact gtctcaagtt tttgcccaaa
     1561 gatcttcgat gcgaggttcg gcagcgtcgg ctcccggttg agcttgatat tctcggccac
     1621 agtaaacccc gggatcagca tgaactcctg atgcaccatg cctatgccat aggtcatcgc
     1681 ctcccgagga gagcttatat ccgtcggaac gccgtccaga ataatccggc cttcaaagcc
     1741 cccagtgtcg aagatgacct gcatgccgaa gaggatattc atcagagtag tctttccggc
     1801 cccgttctcg ccgacaagag catgtatctc tcccggcctg accgacaaat tgacgtcttt
     1861 gagcacttgc gtcccgaaga aactcttgct tatgccctca agttcaagaa cattcccttc
     1921 agttattgac attgtcaagc ccctctcgtt agcatgaaag gaatttacgg gcaggcgccg
     1981 ggcggtacta tgcccggcgc caatttcgct gctaaaatag gcctcgccga ctacttgact
     2041 gtgacgccaa agatgatcga atcgatcaag tagcgataga actgcccgcc cgcgctgagt
     2101 ctctcaaact ctatcggtgt gcccgcgatt ccctgaacga cctttcttac ggcgtccatg
     2161 tccttgtaat tgagcttctt ttccactgcc agcttggcaa tttccacacc agcaatggtg
     2221 ttgaggtaac cagtagcgac cggccatgtc gccatcctgc ccgcctgacc cttcttcaca
     2281 atctcggcgt tgatcgcccg gattattgcg gggaaattcc ccttcatgtt gtccgtgatc
     2341 tccagaccga gagcacctgg atagccatgg gtcgggctgg gacagcactg ctcggggaat
     2401 atggcgccgg tcttgatgac cgcagttatc aagggttcct gcatgccgca gttggtcgaa
     2461 aagaacgccg tgtccttccc atactgcccg acctttctgg gcacatcttc cagaatgaac
     2521 tgctgcgccg cagggagtcc gccttcaccc atgggatcag gcgcgtttgc gaacacaaac
     2581 ctcatcccaa gcttcttgca ctcttcctcc atgatgtccc gccgcgtcgc cagatcggca
     2641 taggacatgt gcctcgggaa tgaatagtga acgaacgtct tcgcacctag tttctttgcc
     2701 aaccggacta tggtggtccc acgagctata ttattgggaa tcagggaaac atctgcatac
     2761 ttttcgacta tcggggggtc ttcatgcggt tcgcccagaa cgaagatgat atccggcctt
     2821 atctcgcgaa ccttgcggat agcagccaca gtgcccggaa cggcctggtt gattactatc
     2881 gccttgacct gcctgtcaga ggcgagtccg gtaatctgcg ctatagtcgt ctcctgctcc
     2941 tgcatgaaat tgtcggggta cgaccgatgc agaatgagcc caggatactt ctgtatggcg
     3001 gcctcagcgc ctcggtattc gtcttcgccc tgcgatacgg ttcccgtaac tacgccgatc
     3061 ttgaagtcag cggccccaat agagacactg gcaagcccca aaataagggc cagcgctacg
     3121 gctgcgagga cacttctttt tgccatttcg ctattctccc cctgcagaag tattttt
//
LOCUS       DAOSYL010000123         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_24347_length_3140_cov_8.538412, whole genome shotgun sequence.
ACCESSION   DAOSYL010000123 DAOSYL010000000
VERSION     DAOSYL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24347_length_3140_cov_8.538412"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..899
                     /locus_tag="PLU15_10875"
     CDS             81..899
                     /locus_tag="PLU15_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOO31443.1"
                     /translation="MTRASDNRKPHLRLEVANVSGSHYPQHHIKAMPDEISRYCFVPG
                     DHIRGLKIAERLDNAAQVSANRAIFVHTGTYKGVKMTVCSTGMGGPQAAIAMEELGML
                     GADTFIRVGSAGALQPGMGVGDIAIATAAYRDGGATRRLLPIPFPAAADFHVTRALYD
                     AANESGAVFHLGVVASTDIFYAPADPSFDKMLADAGAICIEMETDTVFILGHVRQFRC
                     GAIFVMDTGDSGVRPDASGPWADHAADTGFLEGEDLAIDIALEAMRRLATEDEK"
     gene            1077..2705
                     /locus_tag="PLU15_10880"
     CDS             1077..2705
                     /locus_tag="PLU15_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOO31444.1"
                     /translation="MNSLLVMVVTFALYILAYNLYGKRLGSKVFQLATKNITPAEKFN
                     DGVDYVPTNKFILAGHHFTSIAGTGPIVGPVLGVIWGWVPALLWVIFGSIFAGAVHDL
                     GALVLSMRHDGKSIGDITGTVINPRARTLFFTIIFFCLWIVIAIFALLMANLFVQYPQ
                     CVFPIWVEIPIAMGLSYYLYQKKGNLAVGSVVAIVLMYLTIWIGLYIPLELTNEVWLT
                     IIMVYIFFASTMPVQKLLQPRDYINALELLVAMVLLIIGIIVRPQPIVAPAVVHAPAG
                     APSMMPFLFITIACGAISGFHSLVSSGTSSKQIASEPHALAVGYGGMLLEAALATLVI
                     IAVCTGVSSETWLETYSNFAKIPTLPTFAEGGARIVGALGIPEAFALNILAVFMVSFA
                     STTIDTATRLQRYVISEFGEDFQIGWMQNKYIAALIAVVSAYLLAFRGSANVIWPLFG
                     ATNQLLAGLALLTVTVYLAENKQPTAYTAVPMVFMIIITTWGMIANLLQYISQSNWLL
                     TTIDAIIILLEVWLIIEAFNVLGSTQKQELKPTA"
     gene            complement(2888..>3140)
                     /locus_tag="PLU15_10885"
     CDS             complement(2888..>3140)
                     /locus_tag="PLU15_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31445.1"
                     /translation="MVVDPAGAMELWTRDDGSSLQSNTGDRLLVLVNFSHELRNVDVW
                     WPGSTTVEVVTRKDELECDSDGRFRFPLPSNEGVILRQR"
BASE COUNT          757 a          908 c          803 g          672 t
ORIGIN      
        1 ccagatatgc cgggtatgac cgtttgcaaa gggcggacat gcccggcaca catgtactgc
       61 aaaaccgagg aggaacgaga atgacacgag catcggacaa tcgcaaaccc catctcaggc
      121 tggaggtcgc caatgttagc ggatcgcact atccgcagca tcacataaag gcaatgccag
      181 atgagatctc ccgatactgc tttgtcccgg gtgatcacat aagaggcctg aagatcgccg
      241 aacgactcga taacgcagct caggtcagtg ccaaccgcgc catattcgtt cacacaggaa
      301 cctataaagg agtgaagatg accgtctgtt ccacagggat gggagggcct caagccgcca
      361 tagccatgga agagctgggc atgctcggcg cagacacctt tatcagagtg ggctctgccg
      421 gcgccttgca gcccggcatg ggagtgggag acatcgcaat cgccaccgca gcctaccgcg
      481 atggaggcgc aacaaggaga ctccttccca tcccgttccc cgccgcggcc gatttccatg
      541 tcacccgcgc actgtacgac gcggccaacg aaagcggcgc cgttttccac ttgggcgtag
      601 tagcttccac cgacatcttc tacgcgcccg ctgacccaag tttcgacaag atgcttgctg
      661 acgcgggcgc aatctgcata gaaatggaga cagataccgt attcatccta ggccacgttc
      721 gacaattccg ctgtggcgcc attttcgtca tggatacagg agacagcggc gtcagacctg
      781 acgcctcggg gccatgggct gaccatgcag cagacaccgg gttcctagag ggcgaagacc
      841 tcgcaatcga tatcgctctt gaggcaatga ggcgattggc gacagaagat gagaagtagc
      901 gtaaaggtaa gactcttccg tatcaagggc ttgccatatc ctccatgaag tgcatgccaa
      961 atggtataca tatgtagatt gtttagtaaa tatagcagga actcggcaga agatggcgaa
     1021 tacctgtccg ggcagcgcct gaacgcgccc cctaatgata ctgggaggtg gactaggtga
     1081 attctctgtt ggtgatggta gttaccttcg ctctgtacat cctcgcctac aacctgtatg
     1141 gaaagagact gggctctaaa gtgttccagc tcgctacgaa gaacatcact cctgctgaga
     1201 agttcaatga tggtgtggac tacgtcccga ccaataagtt catcctcgct ggtcaccatt
     1261 tcacatcgat tgcgggaacc gggccaatcg tgggaccggt tctcggcgtg atatggggct
     1321 gggtacccgc gcttctgtgg gtgatcttcg gatcgatatt cgccggagcc gtccatgacc
     1381 taggcgcatt agtcctgtca atgcgccacg acggcaagtc aataggcgac atcaccggaa
     1441 cagtcatcaa tccccgtgca cggaccctat tcttcacgat catcttcttc tgcctgtgga
     1501 tcgttatcgc catattcgcc ctgctgatgg cgaacctctt cgtccaatac ccacaatgcg
     1561 tcttccctat ttgggtcgag atacccatag ccatggggct ctcgtattac ctgtaccaaa
     1621 agaaggggaa tctcgccgtc ggctccgtag tggccatagt attgatgtac cttacgatct
     1681 ggatcgggct gtacatcccg ctagaactga ccaacgaagt ttggctcacc ataataatgg
     1741 tctacatctt cttcgcatca acaatgccag tccaaaagct gctacaaccg cgagactaca
     1801 tcaacgcgct cgaacttctg gtcgccatgg tactcctcat cataggcatt atcgtaagac
     1861 cgcaaccaat agtcgcgccg gcagtggtcc atgcgcctgc aggagcaccg tcaatgatgc
     1921 cattcctgtt cataacgata gcttgtggtg caatatcggg gttccattct ctggtgtcga
     1981 gcggcacaag ctcgaagcag atagccagcg aaccgcatgc ccttgcagta ggatacggcg
     2041 gaatgctgct cgaagcggcc cttgcaactc tggtaataat tgctgtatgc acaggcgtct
     2101 cctccgaaac ttggctcgag acatactcga acttcgccaa gattcctacg ttgcccacat
     2161 ttgctgaagg cggcgccaga atagtaggcg cactcggaat ccctgaggcc ttcgccctca
     2221 acatcctggc agtcttcatg gtgagcttcg caagcacgac aatagacacg gcaacccgtc
     2281 tgcaacggta cgtaatctcc gaattcggcg aggacttcca gataggctgg atgcaaaaca
     2341 agtacatcgc agcgcttatc gcagtagtca gcgcctactt gctggccttc cgcggcagtg
     2401 cgaacgttat ctggccactg ttcggcgcga ccaaccaact cctcgccgga ctcgcattgc
     2461 tcacagtcac ggtatatctg gccgagaata agcagcccac ggcatacaca gcagtgccca
     2521 tggtattcat gatcatcatc accacatggg gcatgatcgc aaacttgttg cagtatatca
     2581 gccagtccaa ctggctgctt acgaccatcg acgcaataat catccttctg gaggtctggt
     2641 tgatcataga agcattcaac gttctgggca gtacgcaaaa acaagagtta aagcccacgg
     2701 catagcaacg aattgcccgg gccagtgtcc cgcttcacgg ttaaaccaag tttggggctt
     2761 ctggcgcgat gcgggcaaac atctttcaca tgtagcttcg ccgcgccccc agatagcgca
     2821 aagcgcggcg aagctacatg aagcacttta acccacagac ggtacatcaa tcccgacaag
     2881 atggcactca ccgctgccgg aggataaccc cctcattgct tggaagaggg aatctgaacc
     2941 gcccatcgga atcgcactcc aattcgtctt ttcttgtgac tacttccacg gtggttgacc
     3001 cgggccacca tacatccacg ttgcgcagct catgggagaa gtttactaac accagcagcc
     3061 gatcgccggt attcgattgg agagaagatc cgtcatctcg tgtccaaagc tccatagccc
     3121 ctgccggatc tacgaccatc
//
LOCUS       DAOSYL010000124         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_24754_length_3099_cov_6.749014, whole genome shotgun sequence.
ACCESSION   DAOSYL010000124 DAOSYL010000000
VERSION     DAOSYL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24754_length_3099_cov_6.749014"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1278
                     /locus_tag="PLU15_10890"
     CDS             241..1278
                     /locus_tag="PLU15_10890"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO31446.1"
                     /translation="MRYISIDEAKPGMILARHIWGPHGQLLLTANTTLKETYIKRLKE
                     FRFSALYVKSYPDEPIENLVGPVKQETLVHARETLKAVTESVIASNRIDFAIVRETVE
                     DIVDQVLSNPNVVYSMADIKAFDDYTYGHSVDVCVLSILCGAHMGMNRYELVDLGSGA
                     MLHDVGKLFTPKEILAKPEPLNADEMEVIHRHPWDGFQVLRKHMPLIPAHVAFQHHER
                     YDGSGYPRGLLDADTLDLAKVVAVADSYEAMTSDRPYREAMMPHQALAELARDAGKSY
                     NPTVIKCFVEVVAAYPLGTVVRLSDGSLATVVGVTKEIYDVQIVSGQSKGKTISIQRD
                     SNDISIVERLQ"
     gene            complement(1299..2576)
                     /gene="serS"
                     /locus_tag="PLU15_10895"
     CDS             complement(1299..2576)
                     /gene="serS"
                     /locus_tag="PLU15_10895"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO31447.1"
                     /translation="MLDLKFVRSNPELVRDSLRKRGESTEILDTLLACDERRRAILVE
                     AEELKRQRNEASAEVGRMKQTGEDASSIVSEMRMVSERIRGLDQELSEVEMRLAEMLL
                     GIPNIPHESVPVGRDESENVVVRKWGEPTAFDFEAKAHWDIGTDLDILDFERASKITG
                     SRFVIYKGAGSKLVRALINFMLDLHTQEHGYTEVFPPFLINRDSAVGTGQLPKFAEDM
                     FKCEGLDYYLAPTAEVPVTNMYRGEILPGDLLPIKHCAYTACFRSEAGSAGRDTRGLI
                     RQHQFDKVELVKFVHPDTSYDELERLVNDAESILQLLNLPYQVTMMCTGDVGFSAAKK
                     YDLEVWMPSYDRYVEISSCSNFEAFQARRADIRFRPAPGERAEFVHTLNGSGLAVGRT
                     IAAMLENWQQPDGSVTIPPRLRGYMGKMEAITG"
     misc_binding    complement(2646..2962)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          763 a          912 c          738 g          686 t
ORIGIN      
        1 ttgacaaatt atgcaacggg catgcgcgag aaatcggcta caacgcaaat gcctccgcct
       61 ccgggtttgc ccaagaatgg gaggccgaag caccagcaag aagccacgcg ttctccagac
      121 gctaagccca attgctgcag caggatgttg cagctctttg tggaaagtat cgatagatca
      181 cgacgagcaa tagcatagca gccgaaatcg ataaaatgca attgacaacg ggggatagca
      241 atgcgctaca tttccataga tgaagccaag cccggaatga tcctagccag gcacatatgg
      301 ggcccgcatg gccagcttct gctcactgcc aacactacac tgaaagagac ttacataaaa
      361 agactgaagg aattccgttt ttcagctctc tatgtaaaga gctatcctga cgaacccatc
      421 gagaatcttg taggtcccgt aaagcaggag accctcgtgc acgccagaga gacactgaaa
      481 gctgtcaccg aatctgtcat cgcttcaaac cgtatcgact ttgccatcgt gcgtgaaacc
      541 gtcgaagaca tcgtcgacca agtgcttagc aatccaaacg tagtctacag catggccgat
      601 atcaaggcct tcgacgatta cacatatgga cactcagtcg atgtctgcgt cctgtcgatc
      661 ctttgcggcg cccacatggg catgaacaga tacgaattgg tggaccttgg atccggcgca
      721 atgctccacg atgtgggaaa gctgtttaca cccaaagaga tactggcaaa acctgagcca
      781 cttaacgctg acgaaatgga ggtcatccat aggcaccctt gggacggatt ccaagtgttg
      841 cgcaagcata tgccactcat ccccgctcat gtggctttcc aacatcatga aaggtacgat
      901 ggctcgggct atccccgcgg acttctagac gctgacaccc tggaccttgc aaaagtcgtc
      961 gccgtcgccg attcgtacga agccatgacc tctgacaggc cctacaggga agcgatgatg
     1021 ccacaccaag ccctggcaga attggcgcgc gatgcaggca aaagctataa cccgacggta
     1081 atcaagtgct tcgtcgaagt ggtggccgcc tacccgcttg gcactgtagt aaggctaagc
     1141 gacggttcat tggccaccgt tgtgggggtc accaaggaga tctacgacgt tcagatagtc
     1201 tcaggccaaa gcaaaggcaa gactatctca atccagcgcg attcaaacga cataagtata
     1261 gtcgaacgcc tccagtaacc ccagtgcaac caagccgttt accctgtgat tgcctccatt
     1321 ttgcccatgt aacctctgag ccgaggcgga atcgtcacag agccatccgg ttgctgccag
     1381 ttttccaaca tagcggcgat cgtccttcct actgcgaggc cagagccgtt caatgtatgg
     1441 acgaattcgg cgcgttcgcc aggcgcaggc ctgaacctga tatctgccct gcgagcctgg
     1501 aatgcctcga aattgctaca tgacgagatc tcaacatacc tatcatagct tggcatccat
     1561 acttcgagat cgtacttctt ggctgcagaa aaacccacat ctccggtgca catcattgtg
     1621 acctgatatg gcaaattcaa caactgcaga atgctttcag cgtcatttac aagcctctcg
     1681 agctcgtcat aagaggtatc aggatgaacg aatttgacca gttccacctt gtcgaactga
     1741 tgctggcgta tgagtcccct cgtgtcgcgg cccgcagagc ctgcttctga acggaaacac
     1801 gcagtgtaag cgcaatgctt tatcggcaac aaatcgccgg gcaagatctc tccgcggtac
     1861 atgttcgtca caggaacctc cgccgtgggc gctaggtagt agtcaaggcc ttcacatttg
     1921 aacatgtcct ctgcaaactt gggcaactgg ccagtcccaa ccgctgaatc ccggttaatg
     1981 aggaacggtg gaaagacttc agtatagcca tgttcttgtg tatgcaagtc cagcatgaag
     2041 ttgattagag cccttaccag cttggagccg gcgcccttgt atatgacgaa cctcgacccg
     2101 gtaatcttcg atgcccgttc gaaatcgaga atatccaggt cagtaccgat atcccaatgg
     2161 gctttggcct cgaaatcgaa ggctgtcggc tctccccatt tccgcacgac cacgttttcg
     2221 ctctcatcac ggcctaccgg aacagattca tggggaatgt tcgggattcc aagcagcatc
     2281 tcggctaggc gcatctccac ttcagataat tcctgatcca aacctcggat gcgctcagag
     2341 accattctca tctcacttac tatgctggat gcatcctcac ccgtctgctt catgcgcccg
     2401 acctcagcag atgcttcgtt tctttggcgc tttagttcct cagcttcaac cagtatagcc
     2461 cgacgccgct catcgcaagc caagagagta tcgagtattt cagtgctctc tccccgcttc
     2521 cgcagcgaat cccttactaa ctccgggttt gaccttacga atttcaggtc cagcatacta
     2581 agactccccc ttcatctatt gcccatcccg cctacaaaaa atcccgcccc ctcgcagctt
     2641 tcgccgccgg gggcgggaca aacttcccgc gttgccaccc cgattggtcg cctggacaag
     2701 cacggcaaac acgcccttag gcgctgggcg cttgatctca ggcaaaccct ctcgtgtccg
     2761 ctataacggg cgatcccgtc acggcttact cttgttcggc cggccgctcc agggtggatt
     2821 cccccactgc ctcttaccgg ttcccaccag cccccacaga ctcggaaaca tctgagcgta
     2881 gcttccgctc aaatggtctg aaggcgcccc cggctctctg caaagaagac tgcgggatac
     2941 tattccctct catcgcgttt gctgtatatg caggagaaac ggtatcctgc aacttcctta
     3001 tatgatatat ccacaatcct gcaaagtcaa ggggggccaa cgaaaatgtt acgcgtctga
     3061 gataatgtca cccatgaaca agggggacat catcttgac
//
LOCUS       DAOSYL010000125         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_25132_length_3061_cov_6.541583, whole genome shotgun sequence.
ACCESSION   DAOSYL010000125 DAOSYL010000000
VERSION     DAOSYL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25132_length_3061_cov_6.541583"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(656..1519)
                     /locus_tag="PLU15_10900"
     CDS             complement(656..1519)
                     /locus_tag="PLU15_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO31448.1"
                     /translation="MAGVDNAEFLPIGKMAALNHITVQTLRHYDKIDLLKPLYVDEET
                     GYRYYDIKQSAILDMIQYMKSLGMSLEQIRKQFERKDIEGIRDILRKQSRNIDEEIRK
                     LHQMKRGVEACIRSYDRYLRMPEEGIITLETIPARKVFCYDGKINIYDHGLDTYEYIL
                     RELKKQVIIRDLPMVYFCNVGSILRKEMIDAGRFVSSEIFVFVDDDFEDQEGIEMIPE
                     GNYVCTYFHSFWREKESAEALFRYIDEKGYQIVGDYVCEVVVELPIFDNNERNMYVRL
                     QIPIDPPETSQ"
     gene            1794..>3061
                     /locus_tag="PLU15_10905"
     CDS             1794..>3061
                     /locus_tag="PLU15_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HOO31449.1"
                     /translation="MMNVFSSGACRLPAHHITIRVPWHDSGWAGSVCARPLDNTSCLI
                     LRRIGEGKHDDTEARCAGLRLDSLPAEDLPPCVSERASFMSPFELRRTVLHPYSGIYA
                     QTHGHFAPTSFVHLPYSAACVPFRWMLRKEVEGDPANGQPGIAERLKLGWVPEREPDI
                     RNISGNPVNTNWIQEKDNQLALLDTFFGALRPEESLCFFYAKRTPLSEQSRRVIIGVG
                     RMLSVGEATEYDYTVKNPPLRCVLWERNVGHSIRPGFADGFLFPYQEVLELADREGAN
                     PEEYVAFAPDEYFDSYSYGSELLTHDGAVASLVSCAAALQRIRGRIEGPWDQALEWID
                     KELNRIWRARGAFPGLGSALSAFGYEWGFKHGTLLAYEVELMREATGGSPWDLVDAVI
                     RNKTGRSGPIASLITPGMRKGWKALPTQRRA"
BASE COUNT          631 a          869 c          831 g          730 t
ORIGIN      
        1 attaccttac cctcctctac ctgcggattt gtcaccggcg cgtttctaca aaaagggagc
       61 gccgttgtcg gcgcgaagtc agacaacttg taccccccaa accacccaga atccgaggag
      121 gttgatcaag aaacctgccg caaaacgaat aggatgagcc agaatctggt taggccagat
      181 gggcagatat cggagatcac ggaatcccat cccaagatga acagggtgcc cccgaacccc
      241 cgttggggcc catcatagga gaacgccgta cccagaaatg tccatggatg tgggtaatgc
      301 atagcggcct ggcatcaggt ttgacgaggg ctacgggctg accggcttct gtccgctatc
      361 cggatcgtag ccatgagttg cattacccgg gtttagctgc aggtttgccg gacgtccttg
      421 atgctgtctg ccgcgagacg cccagatctc tcttcggttc gcaggcctta cgcagaacct
      481 gtcggccagg cgacaagctg ggcgcagaac ctgtcggcca cacgacagga tggaactcgg
      541 gccttggaag tcgatagtcc caagccagcg gcagacttga tgtacccgac ggccccgaga
      601 ggcgaacagg aggctccaga cgaagcaggc ttacccggcc aacgcggcct caggatcatt
      661 gggaggtttc gggaggatct atgggtatct gcagtctcac gtacatgttc cgctcattgt
      721 tatcgaatat gggcagctcg accactactt cacacacgta gtctccgact atctgatatc
      781 ccttttcgtc tatgtaccga aacagtgctt cagcgctctc cttctcgcgc cagaagctgt
      841 ggaagtacgt gcagacatag ttgccctccg ggatcatctc tatgccctct tggtcttcaa
      901 agtcatcatc tacgaaaacg aatatctctg aggacacgaa ccttcccgca tcgatcatct
      961 cttttcgcaa tatggaacct acattgcaga agtacaccat gggcaggtcc ctgatgatca
     1021 cctgcttctt cagctccctc aggatatact cgtaggtgtc gaggccgtgg tcatagatgt
     1081 ttatcttccc gtcgtagcag aagactttcc tcgccgggat ggtctcaagt gttatgatgc
     1141 cttcctcggg cattcgcaag tacctatcat agctcctgat gcaggcctca acacctctct
     1201 tcatttggtg gagttttctt atctcttcat ctatgttcct gctttgcttc ctgaggatat
     1261 ctcggattcc ctctatgtct ttccgttcga attgcttcct tatctgctcc aaagacattc
     1321 ccaatgactt catgtactga atcatgtcta gtatcgcgct ctgcttgatg tcgtagtatc
     1381 tgtagccagt ctcttcatca acgtacaacg gctttagcag gtctatcttg tcatagtggc
     1441 gtaacgtctg aaccgtgata tggttcaggg cagccatttt gccgataggt agaaattccg
     1501 cattgtccac tccagccaac tgaggtccct ccgtttgcat atcagatgag tctagctgtc
     1561 tactatataa gagttgctct acacgattct attacatcac ttccagtaca acacttccag
     1621 cacatcattc cctgcacatc actccctgca cagcacttcc actagacgag cttccgccta
     1681 tttggcgcct gccgcgaccg actcagggag gattattaag gcaatttgcg aatgaatcat
     1741 aagggctccg tttcgcaagt tgagtggtca gtgccccgca tgaggaggtc atcatgatga
     1801 acgtgttctc gagtggcgca tgccgtcttc ccgcgcacca cattacgatc cgtgttccat
     1861 ggcacgactc gggctgggca ggatcggtct gcgcgaggcc gctcgacaac acaagctgcc
     1921 tgatacttcg tcgcatcggc gagggtaagc atgatgatac cgaggctcgc tgcgccggcc
     1981 tgcgtcttga ctcgttgcct gccgaggatc ttccgccttg cgtgagcgaa cgggcgtctt
     2041 tcatgtcccc ttttgaactg cggcgcaccg tccttcatcc ctactcaggc atctatgcgc
     2101 aaacccatgg gcatttcgcg cccacctctt ttgttcatct gccctattcg gcggcctgcg
     2161 tgccgttccg atggatgcta cgaaaggagg tcgaggggga tccggctaat ggtcaacccg
     2221 gaatcgctga acggctgaaa ctgggttggg tgcccgagcg tgagcccgac atccgcaata
     2281 tcagcggtaa cccggtaaat accaactgga ttcaggagaa ggacaaccag ctggcgttgc
     2341 tcgacacttt ctttggcgct ctgcgtccag aggaatcact ctgcttcttc tacgccaagc
     2401 gaacccccct gtcggagcaa tcgcggcgtg tgatcatcgg ggtagggcgc atgctgtctg
     2461 tgggagaggc gacagagtat gactatacgg tgaagaaccc accgctccgc tgcgtgttat
     2521 gggagcgaaa cgttggccat tccattcgtc cgggctttgc cgatggcttt ctgtttccgt
     2581 accaagaagt gcttgagctt gctgatcgtg agggcgcgaa ccctgaggag tacgtggcct
     2641 ttgcgccgga tgagtacttc gattcgtact cctacggctc cgaactgctc acccatgatg
     2701 gagccgtagc ttcgctagta tcctgcgcgg cggcgctgca gcgaatccgt gggcggattg
     2761 aagggccgtg ggatcaggcg ctggagtgga tcgataagga gctcaaccgg atatggaggg
     2821 ctcggggtgc gttccccggg ctggggtcgg cgctctccgc cttcggttat gagtggggat
     2881 tcaagcatgg aactctgctc gcctacgaag tcgagttgat gcgcgaggct acaggaggca
     2941 gcccgtggga cctggtcgat gcggtgattc ggaataagac tgggcgcagc ggccccatcg
     3001 ccagtctgat caccccaggg atgcgtaaag gctggaaggc tctcccgaca cagaggcgcg
     3061 c
//
LOCUS       DAOSYL010000126         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_27008_length_2883_cov_7.094059, whole genome shotgun sequence.
ACCESSION   DAOSYL010000126 DAOSYL010000000
VERSION     DAOSYL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27008_length_2883_cov_7.094059"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1672..2163
                     /locus_tag="PLU15_10910"
     CDS             1672..2163
                     /locus_tag="PLU15_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31450.1"
                     /translation="MTSRLAVGLGIFLTIMIVSAAVCGAVPQSVTHNITICVPAVDVV
                     RLSCPGVHATLQARNDNTGFVQHVLEAGDLRWITNDSPREVTVNAQCIPNGLTLEVLK
                     SLSPANKWLELGASHVLLIDDASCHPGTSLPREFSIFYRILATLEVRPDTYEIVVIYT
                     LTV"
     gene            2307..2786
                     /locus_tag="PLU15_10915"
     CDS             2307..2786
                     /locus_tag="PLU15_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31451.1"
                     /translation="MSRRKVMWSVLLLVLLGFSAISFAADSASHTVTIVVPEIDKIWA
                     DAGGTLQLDWLPIEEVDDNMVAYPHTLLQVCLMYVTNASNRKITAQASIPFAGLSLSV
                     KGPGMGYCEELGAGSVTVVDSCIPGSVQTVTLTYDGKADITVPAGDHDVTVTYTITD"
BASE COUNT          646 a          719 c          790 g          728 t
ORIGIN      
        1 tcccgaatta tccccatgat gacccatgtt ggtcggcggg gcccgacaga tgactaaaaa
       61 ccgggcggga aggcagacag tgtgccagct ctctcgccac tgcaggtctt gctgtgctca
      121 tggccaatag cgcggttgat cgcggaggtc ttcattaact gtctgccgca gctgatgaag
      181 acctgactag atactcatgg ggctgtctgc ggggagtatg caattgtgcg aatcagcgaa
      241 gcgcggaaga gaccgccact gcgcgcttag ccatttccgc atggaacgga actcgtctct
      301 caagatagtt tgcacagcta atgccacagg ccctgcggga gtctttgggt tgttcgaacg
      361 tccgtgttcc gattcccgcg gaagattccc ggcagaggtg attctggttc gttataggcg
      421 agggctttgg gttcatgcac tagtcgatgt gggaagagct gaagcaggca attgtatgaa
      481 gcccttggaa ggactcgtgg gttatcggtt attctcgatc cgtggggact cgcatcactc
      541 aaagttctat cctacacaca ttccacccca aggtatcagc gcgaacttca catactcagt
      601 ctctattacg ctgttttcgc cgaatagcgt gatgactatc tctaagaatc gaaaggagct
      661 agaaacaaga gcccatcgca tattcccgcg atcttcacac tgcaagcctt cttgcagcat
      721 tggccgcact tgaattctac gggccaaggc catccgccat tgagccgtca aagtcccgat
      781 cgaatggcgc cgcagcgatt ccttgggtgt cttgtttgca tgatactcga ctatcaccac
      841 ggtatacact gacggaaggg caggttaccc cggaagtcat ggctgatgcg gggctcttcc
      901 tgtatggtgc aggttcatgt ttcgagtgcg cccgacctgt cccttggttc ttcttgtcgc
      961 tccctgctgg ccatgtatct cgtcacgtaa tttggtttgc atgatatgtc tgacgaagag
     1021 aagcccatgc acgccatgat tgcctggcaa gggctggcgc tctgccctca aggctgttcg
     1081 atacagccct gcgactgctg gctacactat tccggaaaac ccatatcctc ccttcatgtc
     1141 gagcccggcg attctcgtgt gttcgtttcg atgagagggc cttcgaacct cctgcctagg
     1201 cgatgctttt ccttgctgtg tgcttcgctc agaggtacta gctggttttc cggcccctat
     1261 tacccacaag gttgagaggc gaggtgcgat tggtaccagt cgacaccctc cttcgtttat
     1321 atacattttc aagaatcatc ccgtgtgata gatcgctgat gttatataag agccaagcaa
     1381 gaccgcgcat ttccgatagt gggcgaccga caagaaacga atgaccttgc atatctcggt
     1441 acctcgagtg aagatggaga aatatcgcgt aaaagacagg ctgtgcgaat cgccagatac
     1501 aaaccatgtt tcggaatgca cgtatcggaa tgcctcgttt gcgaatccat aggattcgac
     1561 aagctgtcaa tgggatttgc tatagactgc gattgtctca tcatgaaatg gcacaattca
     1621 gcgttttcta aagagaagct atgtttcagt gtaagaagga ggttaagtat cttgactagc
     1681 agattagcag taggtttggg gatttttctc acaattatga tagtatcagc ggctgtctgc
     1741 ggcgcggttc cacagtctgt gactcacaat atcacaatat gtgttccagc cgtggacgtt
     1801 gttcggctga gttgccccgg cgttcacgct actttgcagg cacgcaatga taatacagga
     1861 tttgttcagc atgtgctgga ggcgggcgac ctcaggtgga taacaaacga ttctccaagg
     1921 gaggtaacgg tgaatgcgca atgcattcca aatggtctca cacttgaggt gctcaaatcg
     1981 ctcagccctg ccaataagtg gctggagctt ggtgcgtccc atgtgcttct gatagatgat
     2041 gcatcatgcc atccaggcac gagcctgccc agggaattct caatcttcta taggatcctg
     2101 gctacgttgg aggtccgtcc tgatacctac gagattgttg tcatttatac gttgacagtt
     2161 taggcacact tgacaatccc acggcaacga gggcgtgagc cagcccggtt acatactggc
     2221 tagaggctta ggagattgaa gtgagcgggg tggccaactg cgaacttcaa attgagaata
     2281 gtcaagaatg ggaggtcttt tgtcaaatgt ccagacggaa agtaatgtgg tcggtccttc
     2341 tcttggtgct tctggggttc tcggcgattt cgttcgcggc ggatagcgcc tcgcacaccg
     2401 ttacgatcgt ggttccggaa atcgacaaga tctgggcgga cgctggcggg acgctacagc
     2461 ttgactggct gccaattgaa gaggtcgacg acaacatggt tgcgtacccc catacgctcc
     2521 ttcaggtttg tctgatgtac gtcaccaatg cctctaatag gaagataacg gctcaggcaa
     2581 gtataccgtt tgctggcttg agcctgagcg tgaagggacc aggaatgggt tattgcgagg
     2641 agctcggcgc tggttccgta actgtagtag attcctgcat acctgggtca gtgcagacag
     2701 tgacgctcac ctatgatggc aaggcggaca ttactgtccc ggcaggcgat cacgacgtca
     2761 ccgtgaccta tacaattact gactaacagg ctagatcact tggctttaag atgtatggga
     2821 gaggctgggg ggttcttcct cggcctttcc catcggcagc cctgtgcggg tactggctga
     2881 aga
//
LOCUS       DAOSYL010000127         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_27326_length_2854_cov_5.363344, whole genome shotgun sequence.
ACCESSION   DAOSYL010000127 DAOSYL010000000
VERSION     DAOSYL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27326_length_2854_cov_5.363344"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLU15_10920"
     CDS             <1..444
                     /locus_tag="PLU15_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31452.1"
                     /translation="DGYPVAYVSRVEQYSQMMASIHMTMLIAMFVAFLFFLAAGSMLY
                     FRFFLELQEDQAKYIALRRIGLSWREVRGIVTREMAVMFFTPWLVAFVHQMVALRSFS
                     TVTPMMPIEVWKYGATAAGIFLALQTVYFLLARAAYLQELAPAVK"
     gene            776..1696
                     /locus_tag="PLU15_10925"
     CDS             776..1696
                     /locus_tag="PLU15_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO31453.1"
                     /translation="MKRGYRSIRLVLFMVVLVGIAVIAMGCNGNGDKEKTYSVSGRVT
                     ARNTGLGVSGAVVSFGSYGTVTTDASGYWVKSGIKGTVTISVAKEGWRFEPSYIAVSS
                     ETSDVYFQGQELEYRGLTPKARWDYRVEQTVAGYPKETGYMQRGVNMYEVVGDRHLYY
                     ISHDSDIWLHPTEPSVVSPLKSMAAAFDFDHIISRVGNKYYALLSRNDPGYLMFTAPL
                     NIGDTCADLIWVDLFRLDVMRRENITTPAGTYNAWFCTAKYIEWNSTYRVSMWFAPYV
                     GPVKFSIEAEGYVSTGDLKSMVLTLQSYSE"
     gene            complement(1891..>2854)
                     /locus_tag="PLU15_10930"
     CDS             complement(1891..>2854)
                     /locus_tag="PLU15_10930"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO31454.1"
                     /translation="GRLCLLQSLPSLSGRDIYWGPDVGLESQRQELRVRGTCVLGARC
                     EGLIVRGMRRLHAAMPVRSRHRWADAAGRACVWRVSNAPALARRKGLEARVCGEIERR
                     PNMKVDIGSRKPEVFKEYWPGQYEFFSHFEYACGIPHVLFVITTVKENGKPNACFHSW
                     SAFAGDEGGFFAVMPGLGQNTHTYTNIMRTGEFCVNFLGPHYYDACMKTIHDNSDDAD
                     ELAVGGFAPEKSANISAPRIAESFLSLECTLESTTDLSGKGITSLVVGRVVSIAADEN
                     YAKGLDEKYGPSGFMFNIHAPKNLMTGEGDTTAVATLSALRTVD"
BASE COUNT          642 a          806 c          743 g          663 t
ORIGIN      
        1 gatgggtatc cagttgcgta cgtgtcgcga gtcgaacagt actcgcagat gatggcgtca
       61 atccatatga caatgctcat cgccatgttc gtggccttcc tcttcttcct ggcggcaggg
      121 tcaatgctgt acttcaggtt cttcctggaa ctccaggagg accaggcaaa atacatcgcc
      181 ctccgtcgga tcggcctgtc gtggcgggaa gtccgcggaa tcgtaaccag ggagatggct
      241 gtgatgttct tcaccccttg gctcgtcgcg ttcgtacatc agatggtcgc gctgcgttcg
      301 ttctcgaccg tcacaccgat gatgcccata gaggtctgga agtacggagc aaccgccgca
      361 ggcatcttcc tcgccctgca gaccgtctat ttcctgctag cgcgggcagc ctacctgcaa
      421 gaactggccc cggctgtgaa gtgaacccgt ccactgcatg catccgtcga aaagcctccc
      481 cgttaggcca ctgatagtac agatctcctc tgcatcgcca tgccccgctc caatggcgac
      541 aatatgactt caggcgttat tcgagcaata acagcatttt catgcattgc gttcaggatt
      601 catcggaaca tgccgagagg aaattcgagc tacatagcga attgactgta caaacacccc
      661 gtgtcttatc tgcatgttta tgcacaccac tgtatgtata cccggcggcc atcagtgtga
      721 gagcggtgca tcattgatgg tctagtattc tattgcctaa tgaggagggt tttctgtgaa
      781 acgtggctac cgaagtatcc ggctggttct cttcatggtg gttctcgtag gtattgcagt
      841 gatcgctatg ggctgtaatg gcaacggaga caaggagaag acgtactcgg tgtccggcag
      901 ggtgactgcc agaaacacag gtctaggcgt gtcgggcgca gtagtatcgt tcgggtcata
      961 tggcacggtt accacagacg catcaggcta ttgggtcaag agcggcatta agggcacagt
     1021 caccatttca gtcgccaaag agggatggcg gttcgaacct tcgtacatag cggtaagcag
     1081 cgagacgtct gacgtgtact tccagggcca ggaactcgag tacaggggcc taacccccaa
     1141 agctagatgg gactataggg tcgagcagac agtggcaggc tacccaaagg agaccggcta
     1201 tatgcagcgt ggtgtgaaca tgtacgaagt agtgggcgat cggcatctct actacatatc
     1261 tcatgactca gatatctggt tgcaccccac tgaacccagt gtagtctctc ccctaaaatc
     1321 catggccgcc gcttttgatt tcgaccacat catcagccga gtaggcaaca agtactacgc
     1381 actactgagc cgcaatgacc ccggttatct gatgttcacc gccccgctga atattggtga
     1441 tacttgcgcg gatctcatct gggtagacct cttcaggctt gatgttatga gaagagaaaa
     1501 catcactacg cccgccggca catacaatgc gtggttctgc acagccaaat acatagagtg
     1561 gaactctacc tatcgggtta gcatgtggtt tgccccatac gttgggcccg tgaagttctc
     1621 aattgaggcc gagggctacg ttagcactgg cgacctgaaa agcatggtgc tcacactgca
     1681 atcgtacagt gagtagtacc gaagcattat gtgagacagg cgtctaggcc atgtggatag
     1741 aattcgggtt ccgaatatga actttcgcgt gatctacgag cgcgctcatc ccgattatga
     1801 gcgcgctcgc aaacctgata atgtgtttca taatgtacac gttcgggccc tctgaactgc
     1861 cggtgcgctg ccggcaatac tattacgcag ctaatcgact gttctcaggg cgctcagcgt
     1921 cgcaacggct gttgtgtctc cctcgcccgt catcaggttc ttgggcgcat ggatgttaaa
     1981 catgaacccg ctcggaccgt atttctcgtc caacccctta gcgtagtttt cgtcggctgc
     2041 gatactcacg actctgccca caaccagaga agtgattccc ttccccgaca aatcagtcgt
     2101 cgactcgaga gtgcactcca gcgacagaaa gctttctgcg attcgaggcg ccgatatgtt
     2161 tgctgatttc tcaggggcaa acccgcccac ggcaagctcg tctgcgtcat ctgaattatc
     2221 atgaatcgtc ttcatgcagg cgtcgtagta gtgcggtcca aggaagttga cgcaaaattc
     2281 tcctgtcctc atgatgttcg tgtatgtatg cgtattctgc ccgagcccag gcatcacagc
     2341 aaagaagccg ccttcgtcgc cggcaaacgc gctccacgag tgaaaacacg cattgggctt
     2401 gccgttctcc ttcactgttg tgatgacaaa caggacgtgc ggtatgccac aggcatactc
     2461 gaaatgcgag aagaactcat actgacccgg ccaatactcc ttgaacacct caggcttcct
     2521 gctgccgata tcgaccttca tgttaggtct cctttcgatt tctccgcata ctctagcttc
     2581 gagccccttc cgcctagcaa gcgccggcgc attgcttacg cgccaaacac acgcacggcc
     2641 cgccgcatct gcccaacgat gtcgactccg aacgggcatc gcggcatgca gtctccgcat
     2701 tccacgcacg atgagccctt cgcaccgagc gccaagtacg caggttccgc gtactcggag
     2761 ttcctgtctt tggctctcaa ggcccacatc aggtccccaa tagatatccc ggccggacag
     2821 ggaaggcaat gattgcagta aacacaggcg cccg
//
LOCUS       DAOSYL010000128         2797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_28057_length_2797_cov_8.256747, whole genome shotgun sequence.
ACCESSION   DAOSYL010000128 DAOSYL010000000
VERSION     DAOSYL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28057_length_2797_cov_8.256747"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..544)
                     /locus_tag="PLU15_10935"
     tRNA            complement(469..544)
                     /locus_tag="PLU15_10935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Glu,seq:ttc)
     gene            complement(656..>2797)
                     /locus_tag="PLU15_10940"
     CDS             complement(656..>2797)
                     /locus_tag="PLU15_10940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO31455.1"
                     /translation="WIVVNVRNNLAEQEELMRDAEERSLKLLDYLDATERLNLKGIMR
                     IIETALLGRDGQDGGHVDLMKVQMLVDVFGCELPVAITDFGGNVLASSFPLDNEELVR
                     ENPQVRAAADTGKISFGSFQGILGPDKYGVIVAYPVIDPSYGIRSIIVGFVDEERLAD
                     PLRGLELPADAVFEVADLDGNVVARYSRAKEDMRRGEAMEQAMLKSGAAEISERSRSG
                     FMKLEDGSMQLVAEKIIVDPCGRPTLRVAVTTPAASSLSRMNDHILRDLKALLVVAAI
                     ITPVLWGIFRAEVVVPVEKLANLASRLAQGEFGIRHGEPYRRGELGLLARTLDQMSTA
                     LERRANELIYLSHHDSLTGAYNRTYIESIEEDLNAEDKLPTTVIVGDINGLKRINDIY
                     GHSVGDEVVIAAGNIFREYVGEKGIVARWAGDEFTAVLPNTDWKEGLVVCREISHSCS
                     QMEPDPVAPNIALGVATRATLEQSLRDTWIAAEDRMYTNKLAGSTSGRGRLLHSLRQA
                     LAESTHETQQHSSRLHFLATELGKVMGLSAGALDDLALLADFHDIGKIGIPDAILQKP
                     GPLTPPEWDMMMAHPEIGARIVGGCYELEHIADAILSHHERWDGTGYPNGLAGDEIPL
                     ISRILAIVDAYDAMTSDRPYRTAMSRKEALKEIQRCSGTQFDPNLVEVFVKMMEEIDL
                     KEEYEKNWSEGDPYWLCTTLDESKLIGDGGF"
BASE COUNT          665 a          817 c          636 g          679 t
ORIGIN      
        1 tgtgtctttc cagaacttat tatactacac ttgcgaactc tatcaaatat cctcgttgac
       61 tgtttctgcc cgcagaagca aaacctgcac atgccgtgca cgagctcttc ctgtcacatg
      121 agatcttctg gcggaatcgc tcttgtgcac ataaacgcgc aaacgccgga atggagcaga
      181 tattctttgc gatgaaagcg ccgatgccaa ccagactcgt tccctatagt cagcggcctg
      241 tcccagaaca aggttgaacg aagcgtaatc tccctggcgg tgctgtgcac gccgcaatca
      301 acaattgaca gtgtttgagt gaatctgccg ataagagggt catatggcgt cacaacggct
      361 cagtagcagg ttgccccgct tgaacacagg gaaaaggcag aggagaagcc cgaggatgat
      421 ccgacattcc tcatacggcc aaggagagat gaaaaaaggg cataataatg gcgctcccaa
      481 cgggattcga acccgtgtta ccgccttgaa agggcgatgt cctaggccac tagaccatgg
      541 gagcgcaaac atatatggca aatcttaact taacgcataa gtcttttgct cagctatgat
      601 atcacgtgaa cagcaattcg tcaaaccggc gtcacactgc cggattagga tctgatcaaa
      661 aaccaccgtc gccaataagc ttggactcgt caagcgttgt gcacagccag tatgggtctc
      721 cttcagacca gttcttctcg tattcctcct tcaagtcgat ctcttccatc atcttcacaa
      781 atacctcaac caaattcggg tcgaactgcg ttcccgaaca cctctgtatc tccttaagcg
      841 cctctttcct gctcatcgct gtacgataag gacggtcact ggtcatggca tcataggcat
      901 caacaattgc aaggatccgc gagatcagag gaatttcatc ccccgcaagc ccattaggat
      961 atcccgtgcc atcccacctt tcgtgatggg ataagatggc gtctgcgata tgctccagtt
     1021 cgtagcatcc accaacaatc ctcgccccta tctcagggtg cgccatcatc atgtcccact
     1081 ccggaggcgt gagcggaccg ggcttctgaa gtatagcatc tggaatcccg atcttcccta
     1141 tgtcgtggaa gtccgcgagc agcgctagat catcgagggc acctgcagaa agccccatga
     1201 ctttccccag ttcagtggcg agaaaatgca atctgcttga atgctgctgc gtctcatgtg
     1261 tgctttcagc caatgcttgc ctcagcgaat ggagcaatct tcctctgcca gatgttgatc
     1321 ctgcaagctt gtttgtgtac atccggtcct cagccgcaat ccacgtgtct ctcaaactct
     1381 gctcgagtgt ggcgcgtgtg gcaaccccca aggctatgtt gggcgctact ggatcaggct
     1441 ccatctggct gcaggaatga ctgatctccc tgcacactac caagccctct ttccagtcgg
     1501 tgtttggcaa aacggcagta aactcatccc ctgcccacct agctacgatt cccttctctc
     1561 caacatattc gcgaaagatg ttcccggccg caatcacaac ctcatcgccg actgaatgcc
     1621 cgtaaatatc gtttatcctc tttagcccgt ttatgtcgcc cacaatcact gtagtaggaa
     1681 gcttgtcctc agcgttcaaa tcctcttcga tgctttcgat gtaggttcga ttataggcac
     1741 cggtcaacga atcgtgatgg ctgagataga tgagttcatt tgcgcgtctc tccaatgctg
     1801 ttgacatctg gtcaagagtc ctcgcaagaa gcccaagttc cccacggcgg taaggctcgc
     1861 cgtgcctgat tccgaattct ccttgtgcta gccgactggc taagttcgcc aatttttcta
     1921 caggcaccac tacttctgca cggaatatgc cccacagaac aggggtgatg atcgcagcta
     1981 ccaccaaaag cgctttcagg tcgcgcaaga tatggtcatt catgcgagaa agagaagagg
     2041 cagccggagt tgtaacggcg acgcgcagcg ttggcctgcc gcagggatca actattatct
     2101 tttctgcaac gagctgcatt gagccatcct ccaacttcat gaacccactg cggcttcttt
     2161 cgctgatttc cgctgccccg gatttaagca tcgcttgttc catagcttcg ccacgcctca
     2221 tatcctcttt tgcacgagaa tagcgcgcga ccacgttccc gtccaggtca gccacctcga
     2281 aaacagcgtc tgccggtagc tcgagaccac gcaagggatc ggccaatctt tcttcatcta
     2341 cgaaacctac tatgattgag cggatgccat agctcggatc aatcacggga taggccacta
     2401 tgaccccata cttatcaggg ccgagaatcc cttggaagct gccgaacgag atcttgcctg
     2461 tatcggcggc ggcgcgaacc tgcgggtttt cacgcacaag ctcttcattg tccaagggaa
     2521 aagaggacgc caacacattg cctccaaaat ccgttattgc taccggcaat tcgcatccaa
     2581 atacatcgac aagcatctga accttcataa ggtcaacgtg gcccccatcc tgcccatctc
     2641 tgcccagcag cgcggtttcg atgatcctca tgatgccctt cagattgaga cgctctgttg
     2701 cgtcaagata gtcaagaagc ttcaggcttc gctcttctgc atccctcatc aattcctctt
     2761 gttcagctag attgtttctg acattcacta caatcca
//
LOCUS       DAOSYL010000129         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_28327_length_2774_cov_9.340199, whole genome shotgun sequence.
ACCESSION   DAOSYL010000129 DAOSYL010000000
VERSION     DAOSYL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28327_length_2774_cov_9.340199"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..2704
                     /gene="leuS"
                     /locus_tag="PLU15_10945"
     CDS             188..2704
                     /gene="leuS"
                     /locus_tag="PLU15_10945"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOO31456.1"
                     /translation="MRDRYCFEEIEPKWQAKWEEDQLYKVGEETARPKYYLLEMLPYP
                     SGRIHMGHVRNYSIGDVIARQRSMKGWNVLHPMGWDAFGLPAENAAIEHGADPVTWTR
                     ENIAAMRKQIKRLGFSYDWDREITTCDPSYYKWTQWLFLQLYKNGLAYRKTQKVNWCP
                     SCATVLANEQVVNGACERCDTPVTKRVLEQWFLRITDYAERLLSNLDNMPGWPDKVKV
                     MQRNWIGRSEGVDIDFHVPETGDKLTIFTTRQDTIYGVSYVVVAPEHPLVDKLIEGKP
                     NEAEIRRFVEDTIGQDDIVRTSEETEKVGMFTGAYAVNPANGESVPIWVGNYVLMDYG
                     TGAVMGVPAHDQRDFEFAKKYGLPIKVVITPTGERLDESTMGGAYVDEGVQVNSGQFD
                     GMPSIEARSAIADYLEKEESGRRVVHYRLRDWLVSRQRFWGAPIPIVYCDSCGIVPVP
                     EEELPVMLPEHVKFMPTGKSPLMDCPQFVNTRCPVCGGPARRETDTMDTFICSSWYFS
                     RYTSPKTDDGPWKRADVDYWMPVDQYIGGVEHAIMHLMYARFFTMALYDIGLVGFEEP
                     FTNLLTQGMVLMDGAKMSKSKGNTVDPDEIVNEYGADTARLFVLFAAPPERDLEWSAE
                     GVEGCWRFLQRVWRLVHQYVQAVGDERKNMHERVGVAVVPNPDSLSPEAREVRRAVHG
                     ALKKVTEDIEERISLNTAIAAIMECVNSLYALKDAVLSEQGNAAVMAEAIDILVVMLA
                     PFAPHITEELWHEIGHSGSVHRVEWPKYDPRAIVVDEVEIAVQVNGKVRERLTIPADA
                     TEDEAVAMALASEKVLAAVGSGSVVKTIFVPKRLINIVVK"
BASE COUNT          659 a          617 c          863 g          635 t
ORIGIN      
        1 cgcttgaatg gcattcaaga ggtcgagggt tcgatcccct tcagctccac cattttatta
       61 tccatcgcct cgtcggcatg agatgcttgt gcgtgaaaca aacagggtgg taccgcggat
      121 ataattcgcc cctgacactt ggctgtcagg ggtttttttg ttccctaaag ccgaaggagg
      181 aagaagaatg agggataggt attgcttcga agagatagag cccaaatggc aggcgaaatg
      241 ggaagaggac cagctttaca aggtcgggga ggagactgcc cgtccaaaat actacctatt
      301 ggagatgctt ccgtatccct ccgggcggat ccatatgggt catgtcagaa attactctat
      361 cggggatgta atagccagac aaaggtcgat gaagggatgg aatgtgttgc accccatggg
      421 atgggatgcg tttggcctac ctgctgagaa tgccgccata gagcatgggg ctgatccggt
      481 gacctggacg cgtgagaata tcgcggcaat gcgaaagcag ataaagaggc tcggattcag
      541 ctatgactgg gatcgagaga tcactacatg cgacccctca tactacaagt ggacccagtg
      601 gctcttcctc cagctgtaca agaacgggtt ggcgtatcgc aagacgcaga aggtcaactg
      661 gtgtccgtct tgcgcgactg tgcttgcaaa tgagcaggtt gttaacggag catgtgagcg
      721 gtgcgataca cctgtaacca aaagggtgct ggagcagtgg ttcctgagaa tcactgacta
      781 tgccgagaga ttgttgagca accttgacaa catgcctggc tggcctgaca aggtcaaggt
      841 tatgcagcgc aactggatag gccgcagcga aggtgtggac atagattttc acgtgccgga
      901 gacaggcgac aaactgacga ttttcaccac acgccaagac actatttacg gagtgtccta
      961 cgtagtggtg gcgcctgagc accctctggt tgacaagctg atcgagggga agccaaatga
     1021 ggctgagata cgccgatttg tggaggatac gataggccaa gacgacatag tgcgcacgtc
     1081 ggaagagacg gagaaagttg gcatgtttac tggtgcctat gctgttaacc cggctaacgg
     1141 ggagtccgtc cccatatggg ttggcaacta tgtgctgatg gattacggaa caggggcggt
     1201 catgggcgtt cccgcccatg accagcgaga cttcgaattc gccaagaagt atggattgcc
     1261 cataaaggtg gtaatcacgc ccacaggtga gcgcctagat gagtctacta tgggaggcgc
     1321 ctacgtggat gaaggtgtgc aggttaactc cggccaattt gacggcatgc cgtcgattga
     1381 ggcgagatcg gccatcgctg attacctgga gaaagaggaa agcgggagga gggtggtgca
     1441 ctatcgtctc cgcgattggc tcgtatccag gcagcgattt tggggcgctc cgataccgat
     1501 tgtctattgc gattcttgtg ggatagtccc ggttccagag gaggaacttc cggtgatgtt
     1561 accggagcat gtcaagttca tgcccaccgg caagtcgcct ctgatggatt gccctcaatt
     1621 tgtaaacacc cgatgccctg tctgcggagg acctgcgagg cgtgagacag atacgatgga
     1681 tactttcatc tgttcgtcgt ggtatttttc aagatacacg tcgccgaaaa cggatgacgg
     1741 gccttggaag cgggcggacg tagactactg gatgcctgtg gatcagtata ttggcggcgt
     1801 tgagcacgct atcatgcatc tcatgtatgc caggttcttc acgatggccc tttacgatat
     1861 cggtctggtt ggtttcgagg aacctttcac caacttgctc acgcaaggaa tggtgttaat
     1921 ggatggcgcc aagatgtcca agtctaaggg gaacaccgtg gatcccgacg agattgtcaa
     1981 tgaatacggg gcggatacag ccaggctttt cgtgctgttc gcggcgcccc cggaacggga
     2041 tctggaatgg agtgctgaag gcgtcgaagg atgctggagg ttcctgcaga gagtatggag
     2101 gcttgtgcac cagtatgttc aggcggtagg agatgagaga aagaacatgc atgagcgagt
     2161 gggagtggct gttgtgccta atcccgattc cctcagcccc gaggcgcggg aggtgcgcag
     2221 ggccgtgcat ggcgcgctca agaaggttac tgaggacatc gaagagcgta tcagcttaaa
     2281 cacggcaatt gccgcaatca tggagtgtgt gaactcgctg tatgcgttga aggatgcggt
     2341 gctgtctgag cagggaaacg ctgctgtcat ggcggaggct attgatatcc tggtggtcat
     2401 gttggcgccg tttgcgcctc acatcactga ggagctttgg catgaaatag gtcacagcgg
     2461 cagtgttcat cgcgtcgaat ggccgaaata cgacccaagg gcgattgttg tggacgaggt
     2521 tgagatagcc gtacaggtga atggcaaagt tagagagaga ctgactatcc cagctgatgc
     2581 cacagaggat gaagctgtcg ccatggcgtt ggcaagcgag aaagtgttgg ctgcggtcgg
     2641 ctcgggttcc gtggtaaaga cgatcttcgt tccgaagagg ctcataaaca tagttgtaaa
     2701 atagtcttgt tttgtgatat agaaaattgg ttttttggct aacctcattg cctgtgaggt
     2761 tagccttttg ttcg
//
LOCUS       DAOSYL010000130         2691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_29400_length_2691_cov_9.531866, whole genome shotgun sequence.
ACCESSION   DAOSYL010000130 DAOSYL010000000
VERSION     DAOSYL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29400_length_2691_cov_9.531866"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLU15_10950"
     CDS             <1..938
                     /locus_tag="PLU15_10950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO31457.1"
                     /translation="IMGDGRLRPEWRLIVVLILELLITAMLFRVLWDLTMLSLKVPPG
                     LAPDGRLEFLMETIAGDELLGLASQLIPSIALILAILLALRFIDRKSIEDIGAGFCAR
                     DRKELAFGLGLGALSMTVIFAVLLASKSITLENDLAHPMLSWNLAIGMVVFIFVGLSE
                     ELYSRAYSISALSEIYNRWSAAIVSSVLFSLLHAGNANVSMGGLANVLLVGILFAYMF
                     LRSGSVWMPVGYHITWNYFQGYVFGFPVSGAEVEGAYTVAASRNDFLTGGAFGPEGGL
                     LATTAILAGFYIVWRFTGGRNPWRTREPEGDSELL"
     gene            complement(1141..2241)
                     /locus_tag="PLU15_10955"
     CDS             complement(1141..2241)
                     /locus_tag="PLU15_10955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO31458.1"
                     /translation="MAFDTINLSGLTAPGLVLDVGGGGEGIIGSVLGRQVVAIDILRE
                     ELEESSNDALRLVMDARSMQFLDGSFDEATCFFSLMYMSPESIPRVLSEIFRVLAPGG
                     RLRIWDAEIPPAKPGDDLFIIELKIVTGAGATTCAYGVGWPDKSQNCSAIAHIAQDCG
                     FELLGQAESGQTFYLSLLKPYSNVCEIPTKAGPMCIMRATAKHMHDAYGVLLDSCRRV
                     VERGKRVPFWLFTAEGQQHVAEKIRDCDYFIGFISGSPAAVLWIKWADSCSWEDAGSD
                     RLAGYVYGFGVKQEWAGAGVGRALLEWASSYIASKGRRLVRLECDAGNPTLCAYYEML
                     GFEDRGLTAPGNQLRKYERPSGSKAQCSIANP"
BASE COUNT          571 a          702 c          747 g          671 t
ORIGIN      
        1 ttatcatggg ggatggccgg ctcaggccag aatggaggct cattgtcgta ttgatccttg
       61 aattattgat aacggcaatg ctctttcgcg tcctctggga tctgaccatg ttgtccctga
      121 aggttcctcc tggcctggct ccggatggga ggctggagtt tttgatggaa acgattgccg
      181 gtgacgagct ccttgggctt gcaagccagt tgatcccttc catcgcctta atccttgcga
      241 tcctcttagc tttgaggttc attgacagaa agagtattga ggacatagga gcgggatttt
      301 gcgcaaggga tcgcaaggaa ctagcctttg ggctggggct tggcgctctt tcgatgacgg
      361 tcatcttcgc cgtcttgctc gcctcgaaaa gcatcaccct cgaaaacgat cttgcccacc
      421 ctatgctctc atggaatctg gcgattggga tggttgtctt catcttcgtt gggctgtcgg
      481 aggaactcta ctcgagggcg tattcgatat cggctctgtc tgagatatac aaccgttgga
      541 gtgcggccat tgtatcgtcc gtcctctttt ctcttcttca cgcaggaaac gccaatgtgt
      601 ccatgggcgg actggcaaat gtgctattgg tagggatctt gtttgcctac atgttcttgc
      661 ggagcggcag cgtgtggatg cccgtgggtt accacatcac ctggaactat ttccagggct
      721 atgttttcgg gtttccggtg agcggggcag aagtggaggg tgcctacact gttgcagcaa
      781 gccgcaatga ctttctgaca ggcggggcgt tcggccctga gggcgggtta ttggcgacta
      841 cggcgatcct ggcaggtttc tacatagtat ggcggttcac cgggggcagg aacccgtggc
      901 gcacgagaga gccggaaggc gattctgagc ttctgtagca atgcgggaag gcttagtttg
      961 aacatgatca gcggatgagt atgatcaagg cgaatgttat tcctgactga ccagcgaagg
     1021 atttgattgg agatgttggg cgacatacgg tttttgagcg tgacccaaag aagacggcaa
     1081 ggctgcttgc ggccgtgccc ggattgtaga tactgcgaag gtccggtact atgagttata
     1141 ttagggattc gcaatcgagc actgggcctt tgaccctgac ggacgttcgt actttcgcag
     1201 ctgattgcct ggggctgtca gtcccctgtc ttcaaatcca agcatttcgt agtatgcgca
     1261 aagagttggg tttcctgcat cgcattccag tctgactagc cgcctgccct tggaggctat
     1321 ataagaggat gcccactcaa ggagggctcg tccgacgccg gcgcctgccc actcctgctt
     1381 gactccgaag ccatacacat accctgctaa gcggtcgctg ccagcatcct cccacgagca
     1441 agaatcggcc cacttgatcc agaggactgc tgcgggagaa ccactgatga agccgatgaa
     1501 gtaatcgcag tctcgtattt tttctgctac atgttgttgg ccttctgcgg tgaaaagcca
     1561 aaagggaacc cgctttccgc gttccacaac tcgccggcaa ctgtcaagaa ggactccata
     1621 ggcgtcgtgc atgtgttttg cggtggctcg catgatgcac atagggcctg ctttagtcgg
     1681 gatttcacag acattagaat aaggcttcag caagctcaag tagaatgtct gtcccgactc
     1741 ggcttggcct agcaactcga agccgcaatc ttgagcgata tgggcaatag cggagcagtt
     1801 ttgggatttg tcgggccagc ccactccgta ggcgcatgtt gtggcgccgg cgcctgtcac
     1861 aatcttcagc tcgattatga acagatcatc gccgggcttt gcaggaggta tctctgcgtc
     1921 ccatatccgg agccttcctc ctggcgcaag caccctgaag atctccgaga ggacccgtgg
     1981 gatcgattcc ggggacatgt acatcaggct gaaaaagcaa gtcgcctcat cgaaggaccc
     2041 gtccaaaaac tgcatgcttc tcgcgtccat cactagccgg agcgcgtcat tggatgactc
     2101 ctccagctcc tccctgagga tatcgatggc gactacttgt cttccgagga cagagcctat
     2161 gattccctcg ccgcctccgc ccacgtccag aacgagacct ggcgcggtca gccctgacag
     2221 gtttatggta tcgaaagcca tggtctaatc gcccctccat cactcaagga tgtcgcaatt
     2281 gcagagcact ttcattatgg ttccactttt atagtccggt tcctgcgagc atacagaatc
     2341 tataagggct gcaagttctg agtagaccgc attacatata acgagtgtgc catcagcata
     2401 ggccttatcg attagccctt tggtagcgca tcggggagca ggatgccgac aagagcgtct
     2461 gtgtcaacag agctgatcac gcatgccctc tcattactct gttggtgtcg gtgtcagccc
     2521 ctctcggata tcccacataa cgatcgaatg gagaatcctc aacatacttg ctgagaatgt
     2581 agccttgggg ggagtctcgt atacccgtga tcgagccctg tctttccgcg aacgtctata
     2641 gacgtgctat gaacggccaa tagtaaaaag caaatctgca ccttgataac t
//
LOCUS       DAOSYL010000131         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_29658_length_2671_cov_9.997706, whole genome shotgun sequence.
ACCESSION   DAOSYL010000131 DAOSYL010000000
VERSION     DAOSYL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29658_length_2671_cov_9.997706"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            504..926
                     /locus_tag="PLU15_10960"
     CDS             504..926
                     /locus_tag="PLU15_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31459.1"
                     /translation="MNNLLQLKGHAALLIRKRKALERVILDAAVHTDGCLVEKYRKCG
                     KPGCKCTQGELHGPALYISRRVEGKTRYDYVPKDKVDLAKALVGRHTRLYRARARIQK
                     INRDLNGIFDQIVELNRIEFPKKTEKGEKETDGGPTGS"
     gene            904..2409
                     /locus_tag="PLU15_10965"
     CDS             904..2409
                     /locus_tag="PLU15_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO31460.1"
                     /translation="MADQQVLRRDMPGVAEAMKHGEEVVFIESKFGCNDHLLNWLNEK
                     GFWPIWQSTQPDLKRDGGVPPECLNGLFAAMTLSGLEAIQYANPMLTDGRLMAEVGFN
                     LMEVQDKLKNGEGAIHRDTLRNHLKRILIAESKRAFYEHVALMRNRKWLRGGVYAADG
                     MKIVVTSGNSYEGCGRVWDPDTRRYVYGYKVVYIFNLSPNRPRIVALAVGPINMDERE
                     LLLEALRDLRAHVCPPKDMIDVLVLDRGYWGARYFSELIGEFGLDIVTLCRSDLAIHR
                     EAWALAQFDGEKRPLVRHIKRVNNQGKELRYVRHIKAVEGIEAWDETGRTSLKLNAVC
                     VREINEDTGEVSSIVFLTTLDVSEKPAQIADYYDLRWNIENRCNRELSQKWNVRRPIG
                     RKLRAIFAQICMSAMCLNAARIYEEQKPKDAEKLRSEMERQGQKSYLLGHGAVVIVPG
                     RLIYATMSYKRWVDLSSLKVARRVGDLVAQGMSVEEALSLISGEQQEEQEA"
BASE COUNT          690 a          676 c          804 g          501 t
ORIGIN      
        1 cagggaatag gccgcgaggg cctcgaagaa gagtagtctt gccgcggcgg aggcgcgcgc
       61 ggcgagcttc tcttcactgg gcctgatgag gcaggcgata ggcgtctcga aaccggcgtg
      121 aagcagggat gttggcagca ctagcgtcaa caccttaaga ttgtcatgac aggcatagcc
      181 caccgtgtag cctggagctc gtcaaccagg aagtgggaca gttcataagg aagtgaatgg
      241 aggggggcgc gaaaacgata gtgtcaatag ggcagcctgg acgaccagcg gcaatgaaac
      301 ggcgccaggc tctagcaacc acaattctta cacgcccccg gcgaaatgcg aaaatgcgaa
      361 acagaggaat tgaacattcg ctaatgtgag ctaaacaggg caaaagcgag ctccagaaaa
      421 aatatatgtc tggagctcgc tttttagcag gaacctggcc tgtgtatgtc gaaatataca
      481 ggtattatct gggaggtggt tacatgaaca acctattgca gctaaagggc cacgccgcct
      541 tgcttatccg caaacgcaaa gccctggaac gcgtcattct cgatgccgcc gttcacactg
      601 acgggtgcct cgtggagaag tacaggaaat gcggtaagcc cgggtgcaaa tgcacccagg
      661 gggaacttca cgggcccgct ctttacatct ctcgccgggt ggagggaaaa acccgatacg
      721 actacgtgcc gaaggataag gtcgacctgg caaaggcgct ggtcggaagg catacgaggc
      781 tgtacagggc aagggcgcgg atccagaaga tcaatcgcga cctcaacggg atcttcgatc
      841 aaatcgttga gcttaaccgg atcgagttcc ccaagaagac ggagaaggga gagaaagaga
      901 ctgatggcgg accaacaggt tcttaggcgc gacatgcccg gggtcgcgga ggccatgaag
      961 catggcgaag aagtggtgtt catcgagtcc aagttcggat gcaacgacca cttgcttaac
     1021 tggctcaacg agaaaggttt ctggcctatc tggcagtcga cccagcccga cctgaaacga
     1081 gacggcggag tgcctccgga gtgtctcaac gggcttttcg cggcaatgac tctatcgggc
     1141 ctcgaggcaa tccagtatgc caaccccatg ctgacggatg gacgactcat ggccgaagta
     1201 ggcttcaacc tcatggaagt gcaggacaag ctcaagaacg gcgaaggcgc cattcacaga
     1261 gacactctgc gtaaccacct gaaacgaatc ctcattgccg aaagcaaaag ggcattttac
     1321 gagcacgtgg cgctgatgag gaaccgcaag tggcttaggg gcggcgtgta cgccgcagac
     1381 ggcatgaaga tcgtagtcac atcagggaac tcctatgaag gatgcggcag agtttgggac
     1441 cccgacacga ggaggtatgt ctacggctac aaggtcgtgt acatcttcaa cctatcgcct
     1501 aaccgaccgc ggatagtcgc actggccgtg ggaccgatca acatggatga acgcgaactg
     1561 ctgctcgaag ctctaaggga tctccgcgcc cacgtctgtc cgccgaaaga catgatcgac
     1621 gtgctggtgc tggatagagg gtattggggc gcaaggtatt tttctgagct cataggggag
     1681 ttcggactgg atatcgtaac gctttgccga tccgatctgg cgatccatag agaggcctgg
     1741 gccttagccc agttcgacgg ggagaaaagg ccgcttgttc ggcacataaa gagggtcaac
     1801 aaccagggga aggaacttcg gtatgtcagg cacatcaagg ccgtcgaggg cattgaagcg
     1861 tgggatgaaa ctgggaggac gtccctcaag ctgaacgccg tatgtgttcg ggagatcaac
     1921 gaagacaccg gagaggtcag cagcatcgtt ttccttacta ccttggacgt atcggagaag
     1981 cccgcccaaa tcgcagacta ctatgacctg agatggaaca ttgagaacag gtgcaacagg
     2041 gagctgagcc agaagtggaa cgtacgcagg ccaatcggca ggaaactgag ggcgatattc
     2101 gcgcagatat gcatgtctgc gatgtgtttg aacgcagcca gaatctatga ggagcagaag
     2161 ccgaaggacg cggagaagct cagaagcgag atggaaaggc aagggcagaa gagctacttg
     2221 cttggccacg gcgcggtggt gatcgtgccc ggcaggttaa tctacgcaac catgagctac
     2281 aaacggtggg ttgatctatc gagcctgaaa gtggccaggc gagtgggcga tctggtggcg
     2341 caaggcatgt cggttgaaga ggcgctcagc ctaatatcgg gagagcaaca ggaggagcaa
     2401 gaggcataag tcccttcagc ggcatcaaat agacccgcag catgcttctt gaacccgggg
     2461 aaacgagagg ctttggcaag ttcccctccg ttcgtcggca ccccccactc acaccttttc
     2521 acaaccagaa atccctcctt acgcagccag cttgggctcc atgtacacct ctcagccgct
     2581 caccctatcg attcccagcc acgttcaatt cctcagttct catttaggtg tttaaaatgt
     2641 ctttccttcg tggtattatt atgttgactc a
//
LOCUS       DAOSYL010000132         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_29856_length_2658_cov_5.739531, whole genome shotgun sequence.
ACCESSION   DAOSYL010000132 DAOSYL010000000
VERSION     DAOSYL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29856_length_2658_cov_5.739531"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLU15_10970"
     CDS             complement(<1..661)
                     /locus_tag="PLU15_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31461.1"
                     /translation="MGIEERREVMRQVEFSGDSGRNDAPKRPQEMDAAAHLIREKGYA
                     PERFGFGPGVDVQGYSPFGIGLAELQGTIGLVEDLESAESWEPKMPSDIEHDARLWPE
                     ECSKSPRWEEREEQMAIEDGMAPVEGTGLLEGEEEFTPALTPEEELTGHEYGTVSHVA
                     MEPMPAPFDVDQPEPELEIAEEGELAAQSVPQLLPEPGAEIIEAMKSEHIDEKKEIGV
                     ET"
     gene            complement(708..1784)
                     /locus_tag="PLU15_10975"
     CDS             complement(708..1784)
                     /locus_tag="PLU15_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31462.1"
                     /translation="MKLLKQWSVPIFFLVLSIGGIAAAGLDINFLIHEIVSRFGRNML
                     LVLSLLIPVMAGLGLNFGIVIGAMAGQTALIIITHYGVEGLPGLLLAAVISVPLAILF
                     GWLSGRILNKAKGREMVTSMIMGFFANGLYQLVFLVLLGTVIPMKNPVLMLSSGVGLR
                     NTVDLIGVANSLDRALDGLWNGLVMGEIGSVPIGPWRIPVCTILVIVLFCLLLSFITR
                     TKLGQEMRAVGQDMNIAQVAGINVDRTRMVAIIISTVLAAWGQLIFLQNIGTLNTYNS
                     HEQVGMFSIAALLVGGASTRRATIWNAIVGVLLFHTLFVVSPLAGQRLLGIPQVGEYF
                     RSFLAYGVIAVALALHAWQSRSET"
     gene            complement(1850..>2658)
                     /locus_tag="PLU15_10980"
     CDS             complement(1850..>2658)
                     /locus_tag="PLU15_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO31463.1"
                     /translation="GLIGAVMSMNWNLQGFAGFFAAIAIALPVAMVAGYIYALVLERV
                     QGQEMMVGTYVGFSIVSAMCMFWLLAPIKNPAMIFAIGGQGLRYTLTLDKTFAKVLND
                     FLAFSVFGVTIPTGLLLFFGLLCVLMSLFYRSKAGLAMRTVGSNPMFAKSSGMNVRYM
                     KMWGVILSTVLAAVGIIVYSQSYGFVQLYTAPLYAAFPAVASVLIGGATLERATVGNA
                     IIGTILFHTLLTIALPVTQTALRGADISEIARMIISNGMILYALTRAERR"
BASE COUNT          644 a          794 c          651 g          569 t
ORIGIN      
        1 gagtttccac gccaatctcc tttttctcgt cgatatgttc ggacttcatg gcctcaatga
       61 tctctgcccc gggctcaggg agaagttggg gcaccgattg agcagccagt tccccctcct
      121 cggcaatttc cagctcaggt tctggttggt cgacatcaaa tggcgcgggc ataggctcca
      181 tcgccacatg agacactgtg ccatactcat gtcctgttag ctcttcctct ggcgtcagcg
      241 cgggcgtaaa ttcctcttct ccttcgagca atcccgtgcc ctccacaggc gccatcccat
      301 cttcaatcgc catctgttcc tcgcgttctt cccatcttgg ggatttggag cactcctcag
      361 gccatagtcg ggcatcatgc tctatatccg atggcatttt aggttcccaa gactctgcag
      421 attccaagtc ctctaccaaa ccgatcgtcc cttgaagctc agcaagtccg atgccaaatg
      481 ggctgtaacc ttgaacgtct acgccaggcc caaagccgaa gcgctccgga gcatatccct
      541 tctcgcggat cagatgggct gcggcatcca tctcctgtgg cctcttggga gcatcgttac
      601 ggcccgagtc gccagagaac tccacctgcc gcatcacctc ccgacgttcc tcaatcccca
      661 tggggcggac ccatagttac tatattcaca ccggggttgt ctgtttccta agtttccgac
      721 ctagattgcc aagcatgaag cgccaaggca acggcgataa cgccatacgc aaggaagctc
      781 ctaaaatact ctcccacctg cggtattccc agcagccgct gtcctgcaag gggagagaca
      841 acgaataggg tgtgaaagag aagcacgccg acgatggcgt tccaaatagt cgcacgtctg
      901 gtcgaggcgc ctccgaccaa gagcgccgcg atggagaaca tgccgacctg ctcgtggctg
      961 ttgtaagtat tgagggtgcc gatgttctgc aggaatatca actgtcccca tgccgccaga
     1021 accgtcgaga tgattattgc aaccatgcgt gtgcggtcca cattgatccc ggccacctgc
     1081 gcaatgttca tatcctggcc cactgcccgc atttcctgcc caagcttcgt tcgggtgatg
     1141 aatgacagca acaagcagaa taagacaatc accagtattg tgcagacggg tattctccat
     1201 ggtccaattg gcacgctgcc aatctccccc ataaccaatc cattccacaa cccatccaaa
     1261 gctcggtcaa gcgaattcgc gactccaata aggtcgaccg tattcctcag ccccacgccc
     1321 gacgaaagca taaggactgg attcttcatc ggtatgactg tgccaagaag aacgaggaat
     1381 accagctggt aaaggccatt ggcaaaaaag cccatgatca tactggttac catctcacgc
     1441 cccttggctt tgttgaggat tcgtccactg agccatccaa agaggatcgc aagggggact
     1501 gagatgacag ccgcaaggag gagacctgga agcccctcta cgccgtagtg ggttatgatg
     1561 atcagagcgg tctgtccggc catggcgcct atcacaatcc cgaaattgag gcccaatcct
     1621 gccattaccg ggatgagaag ggataggacc aaaagcatgt tccttccaaa cctggatact
     1681 atctcatgta tcaggaagtt tatgtcgagt ccggcagcag cgattccgcc gatagacagc
     1741 acaaggaaaa agatcggcac cgaccattgc ttcaggagct tcattccgcc cctccccctt
     1801 gcctgggccg gcttggttac agtggcaact gtcttctcag gcacgccggc tacctccttt
     1861 cggcccgggt cagggcatat aggatcatgc cgttgcttat gatcatccgg gcgatttcgc
     1921 taatatcggc ccctcgcagg gcagtttggg taaccggaag agctattgtg agcagcgtgt
     1981 ggaaaagtat cgttcctatg atggcattac ccactgtagc ccgctccagc gttgccccgc
     2041 ctatcaagac agaagcaacc gccggaaatg cagcatatag cggtgctgta taaagttgca
     2101 caaaaccgta actctgggaa tagacaatga tgcccaccgc cgccagcacc gtcgagagga
     2161 tgacccccca catcttcatg tatcgaacgt tcattcccga agatttcgcg aacatgggat
     2221 ttgaccccac tgtccgcatg gcaaggcctg ctttggaccg gtaaaacagg ctcatcaaga
     2281 cgcaaagaag tccgaagaaa agcaaaagcc cggtgggaat cgtcactcca aaaacactga
     2341 aggccaaaaa gtcgttgagc acctttgcaa aggtcttgtc aagagtgagt gtgtaccgga
     2401 gcccctgccc tccaatagcg aatatcatgg ccgggttctt gataggtgcc aagagccaga
     2461 acatgcacat cgccgatact atagaaaacc ccacgtacgt cccgaccatc atctcctgtc
     2521 cctgaacccg ttcgagaaca agagcataaa tgtagccggc aaccatggca accggcagag
     2581 cgatggcaat agctgcgaag aacccggcaa acccttgaag gttccagttc atgctcatta
     2641 ccgccccaat tagtcccg
//
LOCUS       DAOSYL010000133         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_30336_length_2625_cov_5.424125, whole genome shotgun sequence.
ACCESSION   DAOSYL010000133 DAOSYL010000000
VERSION     DAOSYL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30336_length_2625_cov_5.424125"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(452..673)
                     /locus_tag="PLU15_10985"
     CDS             complement(452..673)
                     /locus_tag="PLU15_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31464.1"
                     /translation="MDRRAWPTFATTQTRFSVSANYTIEKVARKLTGFICNEHYLQEF
                     KNLPSKPPVETAVSGYFSVSGFPEEWQIV"
     gene            1035..1484
                     /locus_tag="PLU15_10990"
     CDS             1035..1484
                     /locus_tag="PLU15_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOO31465.1"
                     /translation="MKKLYRSRTDSMIGGVCGGLAEYLEVDPTIVRLAFVLLGLWSGM
                     GIAAYLILWVIIPYPDTGKASTSEVIREGAAEIRDSARKVAESTGQAFQQSQKPAANV
                     VIGIILVVLGIVFLARNLNVSWLRWLNAGTLMPIILIILGGLLLVRG"
     gene            1510..2427
                     /locus_tag="PLU15_10995"
     CDS             1510..2427
                     /locus_tag="PLU15_10995"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOO31466.1"
                     /translation="MSELDNDGQRKRRTGIVLPSILILIGILLLLNNLGLVDWNVWGA
                     IARFWPLILIAVGLELILGKRSISGAVGFMIILVIVAGSALAFRSPTSRGAITRTRVE
                     KPLSGATSAEIEIDMGVGALYLGSLANSSYLVSGTVETAAGGWLTQDFSITGTKARLV
                     LSENRGFPFFSMGKQPAMKWDLALNEGIPMRLDIRTGVGETRIDLEHVLATNVEVKTG
                     VGKTDLTLPATGDVKVALSGGIGETTIHIPKGVAARIRTKTGIGSVKVYGDYDRINDE
                     YVSPGFNSAENRVDLEVKGGIGSIAIQSQ"
BASE COUNT          630 a          678 c          677 g          640 t
ORIGIN      
        1 gaaggctacc tgtcgctgtg tgtaagcaca ggttcttctt tcctcatccc cgcaggcttg
       61 ctttcatctg tcgccaggtt tctggctcca agatattggt gtgatcaaca atgaagtaag
      121 accacattgg caagggcatc agcccttgct aaaagaccgc gttcacctgc cgccgtattt
      181 cttcaactgc cacagcagag taactcatgg catttttagt ggccatctac tacaatcaag
      241 acgcttttgg gggatgaagt ggtcccgggg agagactcgt acctcgtcga tccgacaccc
      301 cttcgtctct ctcctttcat gtgatgtttc ccccttctat gtcactaccc caacctcttt
      361 ccattaccgg aatttgatct gagcaaaaag gactttgcac ggtcatccag cgcgagctcc
      421 tgcaatcttg gaccatggta tccgtccttg tctagacaat ctgccattcc tccggaaaac
      481 ccgatacgct aaaataccct gataccgccg tctccactgg gggtttgctc ggcaagttct
      541 tgaattcctg cagataatgc tcattgcaga taaagccggt gagcttcctg gctaccttct
      601 cgattgtgta attggcggag actgagaatc ttgtttgcgt tgttgcaaac gttggccacg
      661 ctcgtctgtc catcagagac aaaaaccacc aagattgccc acgcttcaaa tcttttggcg
      721 cgtcgtacct gtttgtgctg taatgattcc gtggccaccg aaaaagtgat taggcatgca
      781 tcaagagaaa catctccttg acctttctgc aatcagagag taatactgtt aatactgaac
      841 ctgcttcatg agcgtctatt atgagcgatt acgagatatg ctacggaagc gaccaacata
      901 ataatggtta gtggaatcat atccgagccc cctgccaggc tttgatgcca gacgtgctta
      961 cccaagcagc aagaacatgt tcatgaaatg cggaaaaaga agagcctgct ttgggcacaa
     1021 gtgaggtgaa cgcagtgaag aagctttacc gaagtcgaac cgattcgatg ataggcgggg
     1081 tatgtggcgg ccttgcagaa tatctggaag tggatccgac cattgtgcgc cttgccttcg
     1141 tgttgctagg tttgtggtcg ggcatgggca tagccgccta cttgattctt tgggtgatca
     1201 tcccatatcc ggacacgggc aaggcctcga cttctgaagt tatccgtgaa ggtgcagcgg
     1261 agatacggga tagcgcccgg aaagtcgcag agtccacggg ccaagccttt caacaatccc
     1321 aaaagcccgc tgccaacgta gtcataggaa tcattctagt cgtattaggg atagtctttc
     1381 tcgcacgcaa tctgaacgtt tcttggcttc gctggctgaa tgcaggcacg ttgatgccaa
     1441 taatcctcat catcctcggt ggcctcctgc tcgtaagggg ataatatcca taagggagga
     1501 gaatgagcca tgtctgagct tgacaatgac ggacagcgga agaggcggac aggcatagtg
     1561 cttccgtcta tcctaatact cattggtatc ctgcttctcc tcaacaacct cggcttggtg
     1621 gattggaatg tttggggagc aattgcacgc ttctggcctc tcatcctcat cgcagtcggg
     1681 ctcgaactga tactgggcaa aagatccata tcaggggctg taggcttcat gatcatactc
     1741 gtaatcgtcg caggatccgc cttagccttt cgttctccga cgtcaagagg cgccattacc
     1801 cgcacacggg tagagaagcc tctgtctggc gcaacgagcg cagaaataga aatcgacatg
     1861 ggcgttggcg ctttgtatct gggctcgctt gcgaattcat catatcttgt cagcggaaca
     1921 gtggagaccg cggcaggagg atggctcact caggatttta gcatcaccgg aaccaaggcg
     1981 cgcctcgtgc tcagcgaaaa cagaggtttc cctttcttca gtatgggcaa acagcctgcg
     2041 atgaaatggg atcttgcact taatgagggc ataccgatga ggttggatat acgcaccggg
     2101 gtcggggaaa cccgcatcga tctcgagcat gtgctggcaa ctaacgttga agtaaagacc
     2161 ggtgtaggaa aaacagatct gaccttgcct gcaaccggag atgtgaaggt ggccctctca
     2221 gggggcatcg gtgaaaccac tatccatatt ccaaagggtg tagcagcccg aatccgcaca
     2281 aaaaccggaa taggttcggt taaggtatac ggcgactatg accgtatcaa cgatgaatac
     2341 gtctcccctg gcttcaattc agctgaaaac cgagttgacc tggaagtgaa aggcgggatc
     2401 ggaagtattg ccatccagag ccagtaatgc cccattgctg gcaaccgtac agtagcaatg
     2461 ccttttggca ccccggcaca caacatggct acattcaatc ggagcagggc tccggccccc
     2521 ggcgcatcat tcagctggaa tcactggcgg cattccgggg aacgtgcgat gccgggccag
     2581 gtctcaggca ggtagtattc cgttgagttg tcctttttcc catgt
//
LOCUS       DAOSYL010000134         2530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_31774_length_2530_cov_7.708687, whole genome shotgun sequence.
ACCESSION   DAOSYL010000134 DAOSYL010000000
VERSION     DAOSYL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31774_length_2530_cov_7.708687"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..2316)
                     /locus_tag="PLU15_11000"
     CDS             complement(100..2316)
                     /locus_tag="PLU15_11000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO31467.1"
                     /translation="MKVNTSRLQCRMMLLILLMAVPLLAASVQNGFSQRKAMVQQIEM
                     QGLDLLGYMESAMSMGLKRNIETIAMVVLGEGEDFDIEAKQRAIDKIVAKTGNRLDIA
                     IIDDRGRILASSTTQFDSALLADKERLQEALLTGQISFGVHKPDHAGGRPGVLLLYPV
                     FDADNSATFVVAALLYEELLFSSLSNVDLPADVAFELVDLNWDVVVHRETSDRALGLT
                     ASPDLGDVRQKLAFSVDGGLSEVSKWEDGVKRLAGIKTVTDYRGNPVFYVTATMPIGA
                     DLTRATILSFYQVVVMLGVSLIVIPVTWGLMNKQFVEPVNRLVDLAEDLGSGHMDARH
                     GEPYEAGEVGELAKAFDEMADALKERSEELEYLSFHDSLTGAYNRTYIESISEELACE
                     ENLPTTIILGDINGLKMVNDTYGHQAGNELIVRAGAIIQQCVGDSGVVARWGGDEFII
                     VLPNAPMRSGLLLCNRIRSACESSGHYPAAPSIALGVATRMAAEQSIMDTLVSAESRM
                     YRNKFLDTSSARGTLLYSLRRALAESTHETEEHSSRMCFLALELGSMIGLSSNTLDDL
                     AILASFHDIGKIGIPDSILQKPGPLTDEEWKVMKAHPEIGARIVGGSYELSHISESIL
                     SHHERWDGEGYPEGVGGDEIPLISRILAIVDAYDAMTSDRTYRKAMSREEALAEIARC
                     SGTQFDPDLVEMFLTMMEEMARSEEDEMDWLTAGMQWYEGLGDCDSGVPKMPEDEA"
BASE COUNT          585 a          762 c          590 g          593 t
ORIGIN      
        1 caccggagat catgagagca ctcagggagc tagagcggag ttagcacacc gggctgaaaa
       61 ggcaagtccg ggcccatggc ccggacgccg cgatgcccac tacgcttcat cttcgggcat
      121 cttgggcacg ccggaatcac agtcgcccaa accttcgtac cactgcatcc ccgcagtcaa
      181 ccagtccatc tcgtcctcct ccgacctggc catctcctcc atcatagtga ggaacatctc
      241 caccaagtcg ggatcgaact gagttccgga gcaacgcgct atctcggcca aagcttcctc
      301 cctcgacatg gcttttctgt atgtccgatc actggtcatc gcgtcatacg catcgacaat
      361 cgcgaggatt cgggagataa gcggtatctc gtctccgcca accccctcag ggtacccctc
      421 cccatcccac ctctcgtgat gggagagaat ggattccgaa atatgcgaaa gctcgtatga
      481 tccccccaca atcctggcgc ctatctctgg atgagccttc atcaccttcc attcttcatc
      541 agtgagaggc ccgggcttct gcaagatact gtcgggtatc ccgatcttcc ctatgtcgtg
      601 gaaactcgcg aggatagcca ggtcgtccaa ggtattggat gacaggccaa tcatactgcc
      661 caactccaga gcgagaaagc acattctgct ggagtgctct tccgtctcat gggtgctttc
      721 cgcgagagcc cttcggagcg agtaaagcag agtgcccctg gctgatgacg tgtcgaggaa
      781 tttattcctg tacatacggc tttcggcgct cacaagcgtg tccattatcg actgttcggc
      841 agccatccgg gtggcaacac ccaaagctat gcttggcgcc gccggataat ggccggacga
      901 ctcgcaagcc gaacgtatcc tgttacacag caagaggccg cttcgcatgg gcgcgttggg
      961 caagactatt atgaattcgt caccgcccca tctggcaaca acgccgctat ccccgacgca
     1021 ctgctggatg atggcgccag ccctcactat tagctcgttt ccggcctgat ggccataggt
     1081 atcgttcacc atcttcaggc cattgatatc gcccaatatt atcgttgtcg gaaggttctc
     1141 ctcacaagcc aattcctcgc tgatgctttc gatgtaagtg cggttatagg ctccggtcaa
     1201 actgtcgtgg aaactgagat actccagctc ctcactgcgt tccttaagcg cgtcggccat
     1261 ctcgtcgaaa gccttcgcaa gctcgccaac ctcgcccgcc tcgtagggtt cgccgtgcct
     1321 cgcatccatg tggccgcttc caagatcttc tgccaagtcg accagtctgt tgactggttc
     1381 cacaaactgc ttgttcatca atccccatgt tacaggaata acgatcaacg atactccaag
     1441 catcaccaca acttgataga aggacaaaat cgtcgcgcgg gtcagatcgg ccccgatcgg
     1501 catagtggcg gtaacataga aaacaggatt cccgcgatag tctgtaactg tcttgatgcc
     1561 cgctaggcgc ttgaccccgt cctcccattt gctaacctcg ctcaagcctc catctacact
     1621 gaatgccagt ttctgcctga catcgcccag gtctgggctt gcagtaaggc ctaaggccct
     1681 atcagatgtc tcgcgatgca ctacaacatc ccaattgagg tcgacaagtt cgaaagctac
     1741 gtctgcggga agatcaacgt tgctcaggga agaaaaaagc agttcttcat aaagaagcgc
     1801 tgcaacaaca aaagtagcac tattatcggc gtcgaagact ggatataaca aaagcacccc
     1861 cggcctgccg cctgcatggt ccggcttatg cacaccgaag gagatctgac ctgtaaggag
     1921 cgcttcctga aggcgctcct tatccgcgag taatgcggag tcgaactgag ttgtggagga
     1981 agcaagaatc ctgcctcggt cgtcaataat ggctatgtcc aacctgtttc ctgtcttagc
     2041 aacgatcttg tcgatggctc gctgtttggc ctcgatatcg aagtcttccc cttcccctaa
     2101 cacaaccatc gctatcgtct cgatgttccg cttcaggccc atgctcatcg ctgactccat
     2161 atatcctagc agatcaaggc cttgcatttc tatctgctga accattgcct tcctctggga
     2221 aaagccattt tggacactgg ctgcaagcaa tggcacagcc atcaacagga tcaacagcat
     2281 catcctacat tgcagacgcg acgtattcac cttcatagca ggtttcttct ccttccagat
     2341 cattggccat gctcaagaag ggtaatcgac gcattcggta caatttgtgg gcccatttgt
     2401 atatatcggc acatatcggc aatccgttga gccaacctcg gagagcttct gcccactgat
     2461 gtcaaataaa aaatgtgtgt ctttccagaa cttattatac tacacttgcg aactctatca
     2521 aatatcctcg
//
LOCUS       DAOSYL010000135         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_32142_length_2506_cov_7.869849, whole genome shotgun sequence.
ACCESSION   DAOSYL010000135 DAOSYL010000000
VERSION     DAOSYL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32142_length_2506_cov_7.869849"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLU15_11005"
     CDS             <1..248
                     /locus_tag="PLU15_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOO31468.1"
                     /translation="LEITAKAILCVNLIDEARQKGIRVNREVLSRELGIPVVLTAARS
                     GEGLDELKRTILHSVGRGEPVAANHQTSVRAREAVIP"
     gene            245..1609
                     /locus_tag="PLU15_11010"
     CDS             245..1609
                     /locus_tag="PLU15_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOO31469.1"
                     /translation="MNPWPKMPETDETHDEAVIELYQRSRDIAELAVTVESSPHADFD
                     RKLDDALTSRALGIPIMIALLGAVFWITVVGANYPSQLLAKALFWGEAKLSAAFITIN
                     APQWLHGVVVLGAYRTLAWVVAVMLPPMAIFFPCFTLLEDLGYLARITFNMDWLFKKC
                     GAHGKQALTMAMGFGCNAAGVTACRIIDSPRERLIAILTNNFAPCNGRFPTLIVLASF
                     ITAAIAGPRLRSGMASLLVVAAVALGICTTLIVSWALSKTLLVGMPSSFILELPPYRK
                     PQILPVLIRSIYDRTLHVLWRAMVVAAPAGAIVWILGNLVIEGRPAIIHMSTFLQPLG
                     HAMGLDGFILTAFILGLPANEIVLPLLIMCYTSSSTLVEVEGMIHLSETLAANGWTWL
                     TAVCTMVFSVLHYPCSTTLITIWRETESRKWTVLSALIPLATAVVTCCIIASAARFCG
                     LAPL"
     gene            complement(1656..2081)
                     /locus_tag="PLU15_11015"
     CDS             complement(1656..2081)
                     /locus_tag="PLU15_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOO31470.1"
                     /translation="MLTPSLEDYLEELCRHLDCHGTVRPTDIALKLGVSMPSVTRALR
                     RLADMGMIAYRSRSDLLLTEEGRIAGDYLVRRNHALCKFLRAIGCDVCIEDQAEAMEH
                     YLMPETIDRIELLTQFLQDNENKTRFRDFCNGASNIVST"
     gene            complement(2365..2451)
                     /locus_tag="PLU15_11020"
     tRNA            complement(2365..2451)
                     /locus_tag="PLU15_11020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2415..2417),aa:Leu,seq:caa)
BASE COUNT          564 a          699 c          619 g          624 t
ORIGIN      
        1 tgctcgagat tacagccaaa gcaatcttgt gtgtcaatct gatagatgaa gctaggcaaa
       61 aaggaatccg cgtgaaccga gaagtcctca gtcgcgaact aggaattcca gtggtcctga
      121 ccgcggcccg ctccggagag ggattagatg agctgaagcg cacgatactg cattcagttg
      181 gtcgaggaga gcccgtggct gcaaaccacc aaacctccgt tagggcccga gaggcggtga
      241 ttccgtgaat ccgtggccaa agatgcccga aaccgatgag acgcatgatg aggcagtgat
      301 cgagctgtac caacgttcgc gcgacatagc agagctagcc gtaaccgtgg aatcatcccc
      361 ccatgcagac tttgacagaa agctcgatga cgcactcaca tcgagagctc tgggcattcc
      421 tataatgatc gctcttttgg gagcggtttt ttggattaca gtcgtaggcg ccaactatcc
      481 ctctcagctt ctggccaagg ctttgttttg gggagaagcg aagctcagtg cggccttcat
      541 aaccatcaat gctcctcaat ggctgcacgg ggttgttgtg ctcggcgcct atcgcaccct
      601 tgcatgggtg gttgccgtaa tgcttccgcc catggcgatc ttcttccctt gctttacttt
      661 actggaagac ctgggctatc ttgcgcggat aactttcaac atggactggc tcttcaaaaa
      721 atgcggcgcc catggcaaac aggccctcac catggccatg gggtttgggt gcaatgctgc
      781 aggagtaaca gcttgcagaa tcatagactc gccacgagag cgattgattg ccatcctcac
      841 gaataatttc gcgccttgca atggccgttt cccaaccctc atcgtcctgg cctcgttcat
      901 caccgccgcc atcgctggcc cgcgattgcg ctcgggcatg gcttcgcttc tggtagttgc
      961 cgcagttgcg cttggaatat gtacgacatt gatcgtttca tgggcactgt cgaaaacgct
     1021 ccttgtgggg atgccgtctt cattcatctt ggaattgccg ccctacagaa aaccgcagat
     1081 actgccagtt ctcattcggt cgatctatga tcgaacgcta cacgtgctct ggcgcgcgat
     1141 ggtagtagct gcgcccgcag gcgcaatcgt atggatcctt ggcaatctcg taatagaagg
     1201 ccggccagca attattcaca tgtccacgtt tttgcagcct ctcggacacg ccatggggct
     1261 agacggtttc atccttacag ctttcattct gggtttgccc gctaacgaaa tagtgctccc
     1321 cctcctgatc atgtgttaca cgtcttcatc gacgctagtg gaagtagaag gcatgattca
     1381 cctgtcagaa acgctcgctg caaatggatg gacatggctg actgccgtat gcaccatggt
     1441 tttctctgtt cttcactatc catgcagcac aaccttgata acaatctggc gggaaaccga
     1501 aagccgaaag tggacagttc tttcagccct gattccgctg gctacagccg tcgtaacctg
     1561 ttgcattatc gcttctgcag cgcggttttg cggccttgca cctctctgac agcactatgt
     1621 tcagcgaaag cttagccaga caaggaaacc gggggctatg tggacacgat attcgatgct
     1681 ccattgcaga agtccctgaa cctcgtcttg ttttcgttgt cttggagaaa ctgggtaaga
     1741 agctcaatcc tatctattgt ttcaggcata agatagtgtt ccatggcttc agcttgatcc
     1801 tctatacaaa catcacatcc tatcgccctg agaaacttgc acagcgcgtg attccggcgc
     1861 acaaggtaat cacctgctat cctcccttcc tctgtcaaca acaagtcgct ccgggatcta
     1921 tatgcgatca tgcccatgtc ggcaagcctt cggagtgccc tggttacaga tggcatagat
     1981 acacctagct ttagtgcgat gtctgtcggg cgcactgttc catggcaatc aagatgacgg
     2041 cacaattcct caagataatc ctctaggcta ggcgtgagca tttcatccac ctacctctcc
     2101 caatcagact ctatttctga cagtcactct atgcgcatcc tccgattcag ttcttgaaaa
     2161 tcgatgccca tagcttactc tgctgtcgcc taacagatgg tccgtctttg tacatcagaa
     2221 tccccggcgc tcgtgtctgc attacgcgca aaccggctac aatgttccct cctgaaagga
     2281 ataacgaaga gacggacacc acatggctat tcgccaatag tgtccgtcac agtttgcata
     2341 tccacatatg taattcgaaa aagatggtgc cgaagggggg actcgaaccc ccatgacctc
     2401 gcggtcggat gattttgagt catctgcgtc tgccgattcc gccacttcgg cgcgtcacta
     2461 ttctaccacg ggcgcccgct tggtgcaata ggacgcagca gtcgag
//
LOCUS       DAOSYL010000136         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_32776_length_2466_cov_5.897553, whole genome shotgun sequence.
ACCESSION   DAOSYL010000136 DAOSYL010000000
VERSION     DAOSYL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32776_length_2466_cov_5.897553"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLU15_11025"
     CDS             <1..754
                     /locus_tag="PLU15_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31471.1"
                     /translation="ADLKIPHGMHVSIPCGQCHGPQLPTEAKAAYAITHDTCLPCHEE
                     EIKDPDKCSTCHKTPDVAETAYLKIPHDMHAGIECGICHGPQTPASAKKAWRISHDTC
                     IACHEDDIKDPAACETCHKTPNVTQTKTLKIPHAAHAGVPCGTCHGPEAPASAKKAWV
                     VSHNTCISCHSKWIGSVDKCEKCHKTPNINETAEIRIPHALHADMVSCGFCHGPQAPP
                     AAKAAFQMGHDQCSDCHDTDDYDTCETCHKWE"
     gene            complement(872..1576)
                     /locus_tag="PLU15_11030"
     CDS             complement(872..1576)
                     /locus_tag="PLU15_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31472.1"
                     /translation="MLVVTDLTARYGDISVIHGISFSIDEGESVAIIGANGAGKTTLL
                     RTLMGLHREFSGTILFNGEDIGRLPAWERSRRGLAMVPEGRRVFPDLTVEENLLIGAY
                     SRADTRETHKSMGEVYALFPILDERRRQVSKTLSGGEQQMLAIGRALMSNPRLLLVDE
                     VSTGLMPIFVDKAFSTLTELNRRGVSILLVEQNARKSLSTMSRGYVLETGRISLAGTC
                     EELSCNPEVQKAYLGI"
     gene            complement(1579..2337)
                     /locus_tag="PLU15_11035"
     CDS             complement(1579..2337)
                     /locus_tag="PLU15_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO31473.1"
                     /translation="MAAARDILSVDHVSKRFYGVQALSDVSLSVKPRTIFGIVGPNGA
                     GKTTLFNVICGAYAPTSGKVLFEGQDITTFSASQTARLGIARTFQIVHSFRNMTVLDN
                     VIVGCGHRVYGRVSHLVGRYGTREIRDKSMALLDLVGLAEHCSTTAGKLPIAQQRRME
                     VARALALDPRLLLLDEPCAGLTYGETESLMELIHKVRQTNVTVIMVEHNMGVIMNLCD
                     EVAVLDFGTKIAQGPPSVIAADPRVIEAYLGKEA"
BASE COUNT          537 a          745 c          663 g          521 t
ORIGIN      
        1 ggcagacctt aagatccccc acggcatgca cgtgtccatt ccgtgcggcc agtgccacgg
       61 gccccagttg ccgacggagg ccaaggcagc ctatgcaata acccacgata catgcctgcc
      121 gtgccatgag gaagagataa aggatccgga caagtgcagt acatgccata agacccctga
      181 tgttgcagag acagcctacc tgaagatccc tcatgacatg cacgcaggca tcgagtgcgg
      241 catatgccat ggcccacaaa cgccggcgtc cgcgaagaaa gcctggcgga taagccacga
      301 cacatgcata gcatgtcatg aagacgacat aaaggatcct gcggcgtgcg agacatgcca
      361 caagaccccc aatgtgactc agaccaagac actcaagata ccccatgccg cgcacgccgg
      421 cgtaccatgc ggtacctgcc atggccccga ggccccggcg tccgccaaga aagcctgggt
      481 tgtcagtcac aatacatgca tctcctgcca ctcgaagtgg atcggcagcg ttgacaagtg
      541 tgagaagtgc cacaagacgc ccaacataaa cgagactgct gaaatccgga taccccacgc
      601 gctccacgcc gacatggtct catgcggatt ctgccatggc ccacaggccc cgcctgcggc
      661 aaaagccgcg ttccaaatgg gccacgacca gtgctcagac tgccacgaca ccgatgacta
      721 cgacacctgt gagacatgtc ataagtggga gtagctgggc gtacacaggc aatagctttg
      781 atgaaaccat atgacgtgaa gatccggagg gcccgtgtcg gcgggccact ccggatctcg
      841 cttcgaaccc aattgtcagg accttcatcc actagatgcc gaggtaagcc ttttgcacct
      901 cgggattgca gcttagttcc tcacaggttc cggcaagtga tattcgcccg gtctccagaa
      961 cataccccct ggacatggtt gatagcgatt ttcttgcgtt ctgttcgact agtaggatgc
     1021 ttactccgcg tcggttcagc tctgtgagag tgctgaatgc cttgtcgacg aatatcggca
     1081 tcagccctgt agacacctcg tctaccaaga gaagccgggg attggacatc agtgcgcggc
     1141 cgatggcgag catctgttgc tcgcctccgc tgagcgtctt ggacacctga cgtctccgtt
     1201 cgtccaggat agggaaaagc gcatatactt cgcccatgga cttgtgggtt tcccgcgtat
     1261 ctgcacggga atatgctccg atcagtaggt tctcctccac tgttaggtcg gggaagaccc
     1321 ggcgcccttc cggaaccatg gctaggccac gcctggaccg ctcccatgcc ggcaagcggc
     1381 cgatgtcctc gccgttgaag aggattgttc cactgaactc cctgtgcagt cccattaggg
     1441 ttcgaagtag cgtggtcttg ccggctccgt tggcgcctat gatggctacg ctctcacctt
     1501 cgtcgatgct gaacgagatc ccgtggataa cgctaatgtc tccataccgt gctgtaaggt
     1561 cggtcacaac cagcattctc acgcctcctt ccccagatac gcttctatca ctctcggatc
     1621 tgctgctata accgacggcg gcccctgggc tatctttgtg ccgaaatcga ggactgcgac
     1681 ttcatcgcag aggttcatga taactcccat attgtgttcc accatgatta cggtaacatt
     1741 ggtctgtctg accttgtgta ttagctccat gaggctctcc gtctcgccgt aggtaagacc
     1801 tgcacacggc tcgtcgagca gaaggagtct cgggtcgagc gccaatgctc tggcaacttc
     1861 catgcggcgt tgctgggcta tgggaagctt acccgcggtt gtgctgcagt gttcagcaag
     1921 gcccaccaag tctaggagtg ccatggactt gtcacggatc tcgcgcgtcc catatcttcc
     1981 gactaggtgc gatactcttc cgtagactct atgcccgcac ccaacaatca cgttgtccag
     2041 gacggtcatg ttccggaacg aatggacgat ctgaaacgtc cgagcgatac ccaggcgtgc
     2101 ggtctgcgat gcggaaaacg tagttatgtc ctgtccctca aagagcacct tcccagaggt
     2161 gggcgcatag gcgccgcaga ttacgttgaa gagcgttgtt tttcctgccc cgttaggccc
     2221 tacgatcccg aagatcgtgc gaggcttcac tgacaagctg acgtcgctta gggcctgcac
     2281 tccgtagaat cgtttgctga cgtgatcgac tgaaaggatg tcacgagcgg cagccatcat
     2341 gcttcacctc caggcgcgca ctgggtcttg cgccgccgcg cagcaactga ccgcagccat
     2401 tcccatatta ggcttccatc gcctatgagg ccgctgggtt gaaatctcat catgagtaac
     2461 agtagc
//
LOCUS       DAOSYL010000137         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_33426_length_2427_cov_8.298904, whole genome shotgun sequence.
ACCESSION   DAOSYL010000137 DAOSYL010000000
VERSION     DAOSYL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33426_length_2427_cov_8.298904"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLU15_11040"
     CDS             <1..245
                     /locus_tag="PLU15_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31474.1"
                     /translation="GLKWMHYFVLVGYDEPTKELHFYDPEIAEDGNGKDPGNRILAEE
                     QFLKLWNTHIPGFRNIYIVCTPSEGRHNSGLNAFKR"
     gene            complement(384..1304)
                     /locus_tag="PLU15_11045"
     CDS             complement(384..1304)
                     /locus_tag="PLU15_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO31475.1"
                     /translation="MGSVDSTVPSYVGLYRTGELARRAEEAVRRLSRCVICAQACNVN
                     RIEGELGFCRTGRLAVVSSAGRHFGEEDVLVGTGGSGTIFFANCNLACVFCQNYDISA
                     CGFGKEVSRRRLADMMLSLQAKGCHNINLVSPSHVVPQVLEALVLAVEEGLRLPLVYN
                     TGGYDALSTLKLLDGIVDIYMPDFKFADADDAERFSAAPRYPTVAQAAIREMHRQVGD
                     LVVDEDGVARRGLILRHLVMPGKLDDTREIMRFVVRDISRDTYVNVMGQYRPAHLASR
                     YPELSRALGISEHQRAIEIAREEGLYRFAR"
     gene            complement(1446..>2427)
                     /locus_tag="PLU15_11050"
     CDS             complement(1446..>2427)
                     /locus_tag="PLU15_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31476.1"
                     /translation="FKRNSTREDAIAGVRWLNEHYRDSSYAVINHPSRAMKYAPSDLR
                     DLNNAGPDVCFGFEGLPGRQKKSFRCGYSQDFAGMTPLCRTYGGADPFLATIGGVWDS
                     LLGEGRRFWVFANSDFHEAGSDSDFYPGEYAKNYVKVDENSPKGIVDGLRSGNCFVVH
                     GDLIDHLDFWAECGSSAATMGESIWVPAGSDVVVTVRLRSPHEDGVDHVDLIAGRVSG
                     LIVPEDARYNVGNNPSTHVVARFFTKGKLLDDEGFASMSHVIRNVRSNMYVRLRGTNL
                     PVGAALETDFDGDPLSDALAHLGGYDNEMEAYHDLWFYSNPINIRVEGAC"
BASE COUNT          615 a          720 c          554 g          538 t
ORIGIN      
        1 atggattgaa gtggatgcac tactttgtgc ttgttggata cgatgagccc accaaggaat
       61 tgcattttta tgaccctgag atagctgaag acgggaacgg caaagaccca ggaaatcgca
      121 tattggcaga agaacagttc ttgaaattgt ggaacactca cattccgggc ttccgaaata
      181 tctacatagt atgtacacca tccgaaggca gacacaatag cggcctgaac gctttcaaac
      241 gataatgcct agatttggaa gcaagctgat gcttgccaac aattcaaaga cagatcagga
      301 accacctaga cgtgcatcaa tggcacctgc gcaacggctt atggcatacg gactcagctt
      361 cgacttcacg aagccagtga agtctacctc gcaaatcgat acagcccctc ctcgcgcgcg
      421 atctcaatgg ccctctggtg ttcggatatc ccaagcgccc tgctaagctc gggataccgg
      481 gaagcaaggt gggcaggtcg atactggccc attacattca cataagtgtc gcgcgagata
      541 tcccggacca caaatctcat tatctcacgt gtatcgtcca acttccccgg catcaccaaa
      601 tgccgcagaa taagcccacg ccgggcaacc ccatcttcat cgactaccaa gtcacctacc
      661 tgccgatgca tctcccttat cgcagcctgc gccacagtcg ggtatcgcgg tgcggcggaa
      721 aaccgctctg catcatcggc atctgcgaac ttgaagtcag gcatgtagat gtcaactatc
      781 ccatcaagca gtttcaaagt tgaaagtgca tcataacccc ctgtgttgta cacaagcggc
      841 agcctcagcc cttcttctac cgcgaggacc aacgcctcca aaacctgggg cacgacatgg
      901 ctcggggaga ctaaattgat gttatggcag cccttggcct gcaagcttaa catcatgtcg
      961 gccaatctac gacgggagac ctcttttccg aaaccgcacg cgctaatatc gtagttctga
     1021 cagaagacgc acgcaaggtt acagttggca aagaagatcg ttcccgagcc gccggttccc
     1081 accaaaacgt cctcctcacc gaagtgccgg ccggcacttg acaccacagc tagccttccc
     1141 gttcggcaga accccagctc gccttctata cggttgacgt tgcaggcttg ggcacatatc
     1201 acgcaacggg aaagccgtct taccgcttcc tcagcacgcc tcgccagctc gcctgtgcgg
     1261 taaaggccaa catagcttgg aactgtagag tcaactgaac ccatcaaaaa actcctttgt
     1321 tttggagcaa taacgctcct atgcagctac ctgtggatta tcataccaca tcataacaga
     1381 tgcaaaacag gaaggggaca tgagatttgg tcttgctctc ggtatgcagc tcccgcgcat
     1441 agcacttagc aagcaccctc aacccttatg ttgatcgggt tcgaataaaa ccacagatcg
     1501 tgataggctt ccatttcatt gtcatagccg cctaggtggg cgagagcatc agatagcgga
     1561 tcgccgtcga agtctgtctc cagcgccgcc cctaccggaa gattcgtacc tcggagcctc
     1621 acatacatgt tactccgcac attccggatc acatggctca tgctggcaaa cccctcatca
     1681 tcaagaagct tgccttttgt gaagaatctt gcaaccacgt gcgtagatgg gttgtttccc
     1741 acgttgtatc tcgcatcttc gggcacaatc aggccggata ctcttccggc gatcaagtcc
     1801 acatgatcca ccccatcttc atggggactt cttagacgca cagtaacaac cacatctgac
     1861 ccagccggaa cccatattga ctcgcccata gttgcggcgc tgctgccaca ctcggcccag
     1921 aaatcgaggt ggtcaataag atctccatgg acgacaaaac agttcccgga cctcaatccg
     1981 tccactattc cctttgggct gttctcgtca accttcacgt agttctttgc gtattcgccc
     2041 gggtagaaat cagaatcgga cccagcctca tgaaaatcgg aattagcaaa aacccaaaat
     2101 ctgcgacctt ctcccagcaa gctatcccac acccctccaa ttgtagcaag gaagggatcg
     2161 gctccgccat atgtccgaca tagtggtgtc ataccggcaa aatcctggct atagccgcac
     2221 ctgaagctct tcttctgtct tcccggcagg ccttcaaagc cgaaacagac gtcagggcct
     2281 gcattgttca gatccctcag atcggacggt gcatacttca tagcgcgcga aggatggttt
     2341 atcaccgcat agctggagtc tcgatagtgt tcattgagcc agcgaactcc agctatggca
     2401 tcctcacgcg tgctgttgcg cttgaat
//
LOCUS       DAOSYL010000138         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_36099_length_2282_cov_7.497081, whole genome shotgun sequence.
ACCESSION   DAOSYL010000138 DAOSYL010000000
VERSION     DAOSYL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36099_length_2282_cov_7.497081"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            738..2264
                     /gene="rlmD"
                     /locus_tag="PLU15_11055"
     CDS             738..2264
                     /gene="rlmD"
                     /locus_tag="PLU15_11055"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOO31477.1"
                     /translation="MVKSDKRRHKGQDDFGSNRRSRAPLDGRRQLKKGLRGDSSVSEA
                     EVDCYAPVGPGQQLALTIHGLTSEGEGVGRANSFAVFVPGALVGEEIVCTVETVKKNF
                     ARAKLDRVIVPSEERVEAVCDLYPACGGCQLLHLSYEGQLAAKRQRVVDAVARIGGLE
                     EVTVHQVIGMVEPWRYRNKVQYPVGCCGDQLVTGCYMSGSHEVVPTEDCYIQHPVTMG
                     VVATVRRLAGEFGLSVYEEKSHRGFFRHVLVKYAYGTGEVMVVLVTNGVGFPGGRGAE
                     FGSRIAAGHREVKSVIQNVNISRGSAVLGRDNVVLWGEETITDTVEDLAFRISATSFY
                     QVNPVQTVTLYRKALEYAGLNGTERVLDAYCGVGTLSLFLARAAREVYGIESVGAAVR
                     DARANADLNGADNVRFIVGRVEDVLPRLVREGLAFDVAVVDPPRSGCDPAVLQALAGK
                     GSGGAGVGRIVYVSCNPATMARDLKIMGGLGYTTCEIQPVDMFPHTFHVETVVLMSRL
                     "
BASE COUNT          506 a          506 c          747 g          523 t
ORIGIN      
        1 gtcgcagtgc ggtaattggt tcgcatttgt gcatcgacaa cgtacacata ttggcattaa
       61 ccataaacgc ccgcagcact cagccgtgcc gggatatgat cctcggggca aagccagggt
      121 agatcgaaat cccggttcga cgctcgcggg caaccatctt ctttacaacg acgaaagcga
      181 tgcgcataag gtccgtgtga gcgccccggc agtttatgca ttcaacgcac tcatatgaaa
      241 gggcaacttg ggcctgcctt caaggaatgt gtctattatt tagtcgcttt tcgggcgcgg
      301 gtgtctaggt tttagactgg tctgcaaaac ctgctcgccc ctgcggcgaa ccttggccgg
      361 ctccacgccg cacggcgctt tcctaggctt ctacccgagg ccaaactctt agcagtcccc
      421 cggctaagtc ccaaaccgcg gcaatacaaa ctcgaggggc gcgcagagat ttgttgcggt
      481 ctacttagat gactgggagt aacgaaattg gccggggcga agagcgattc agcttgaagc
      541 tggcctcggt agagtaagtt gttgatgcga agtcggatgg ggctggttat cccttcagcg
      601 ggagtgcaaa gcttttgcta gatgccgcca accatcaaat gggaccgcac acattggtag
      661 ttgggggcct cctgcaggct atgagggggt aaggggagaa tatgctagtg aaagcagcgt
      721 tccggaggga gattatgatg gttaagtcag acaaacgtcg acacaaaggc caggatgact
      781 tcgggtcgaa ccgaagatct cgggcgcctt tggatgggcg tcggcaactc aagaaaggac
      841 tacggggcga ttcctctgta agcgaggcag aagttgattg ttatgcacca gtcgggcccg
      901 gacagcaact ggctttgacc atacatggac tgacaagtga aggcgagggt gtgggacgcg
      961 ccaatagttt tgcggttttc gtccctggag ctcttgttgg cgaggagatc gtgtgcactg
     1021 tggagactgt gaagaagaac tttgcccgcg ccaagcttga cagggtaatt gttccttcag
     1081 aggaacgcgt tgaggcagtg tgcgatctgt acccggcctg tggcgggtgt cagctgcttc
     1141 acctgagcta tgaaggacag ctggccgcga agaggcagag ggttgtggat gccgttgcca
     1201 gaattggtgg gctggaggaa gtaacggtcc atcaggtaat cggcatggtt gagccgtggc
     1261 ggtacagaaa caaagtgcag tatcctgtgg ggtgttgtgg tgatcaattg gtgacgggct
     1321 gttacatgag cggaagccac gaggttgtgc ccaccgagga ttgctatata cagcacccgg
     1381 tgaccatggg agtagtcgcg actgtgcgta ggcttgccgg agagttcggg ctttctgtgt
     1441 atgaggaaaa gtcgcatcgg gggttcttcc gccatgtgct agtcaagtat gcctacggta
     1501 ctggcgaggt aatggtggtg ctggtaacca atggggttgg tttccccgga ggccgaggcg
     1561 ccgagttcgg cagcaggatc gctgctgggc atcgtgaggt gaagagcgta atacagaacg
     1621 tgaacatatc tcgtgggagc gcggtgcttg gaagagataa cgtagtcctg tggggagagg
     1681 agactataac tgacaccgtc gaagatctgg ctttccgtat atcggcaacg tcgttttacc
     1741 aagtaaatcc cgtccagact gttacactct acagaaaagc tcttgaatat gccgggctca
     1801 atggtacaga gcgggtgctc gatgcatact gcggtgttgg gacgcttagc ctgttcttgg
     1861 cacgcgcagc acgagaggtg tatgggatag agtcagtggg agcggctgtg cgtgatgcca
     1921 gggctaatgc agatctgaat ggagcagata atgtccggtt cattgtcggc cgagttgagg
     1981 atgtgcttcc aaggcttgtg agagaggggc tggcttttga tgtggcagta gtggatcctc
     2041 caaggtcggg gtgtgatccg gcggttctgc aggcattggc aggaaagggg agcggcggag
     2101 caggggtagg tagaatcgtc tatgtgagct gcaacccggc taccatggct agggatctca
     2161 agattatggg tggactgggg tatacgactt gcgaaattca gcccgtggat atgttccctc
     2221 atacattcca tgttgagacg gtagtattga tgtcaagact gtaagcctag attttactgg
     2281 gt
//
LOCUS       DAOSYL010000139         2142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_39135_length_2142_cov_6.514614, whole genome shotgun sequence.
ACCESSION   DAOSYL010000139 DAOSYL010000000
VERSION     DAOSYL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39135_length_2142_cov_6.514614"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..281)
                     /gene="spoIIIAC"
                     /locus_tag="PLU15_11060"
     CDS             complement(84..281)
                     /gene="spoIIIAC"
                     /locus_tag="PLU15_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOO31478.1"
                     /translation="MGLDLVLRIAGVGILASVLCTILKQQDKEEYSLLVATAGLVVVF
                     VMLLDYIEELLSSVRTVFRLW"
     gene            complement(317..859)
                     /locus_tag="PLU15_11065"
     CDS             complement(317..859)
                     /locus_tag="PLU15_11065"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HOO31479.1"
                     /translation="MRVALKIAGAALTIISSGLAGLTVACSYRTHARELAGWVTALAV
                     LETEISYGLTKLGQAMRHAGQSAGGSPKRVLARAADLLEDGGYGSAGDCISKALTEET
                     ASCSFLWSDKWREDLEAVVVLGEALGASDSADQARHISLASRRLERRRQLAEEAATVN
                     YRLWSAMGFLIGGMVAIALI"
     gene            complement(844..1935)
                     /gene="spoIIIAA"
                     /locus_tag="PLU15_11070"
     CDS             complement(844..1935)
                     /gene="spoIIIAA"
                     /locus_tag="PLU15_11070"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOO31480.1"
                     /translation="MRRIVRDLVAEQICPALPPRAREAVKALPDEVRQSLTEIRLRRG
                     RPAMGVVPEGDFYLSSDGQPLWLDRGRAAFSSEIPASAVMCSEDEWDAAMRLVTECSI
                     YALERELAAGYITLRGGHRVGLVGRAVLEGGRIRTQRELSAMNFRIARQIIGAADSIM
                     PYIVRRVGTLQHHRVSVFSTLILSGPGYGKTTLLRDIARQLSYGQNGTGLMVGVVDER
                     SEIAACSGGIPGNDLGPRADVIDSCPKAEGMMIMIRSMSPDVIVTDEIGHAADSEAIE
                     EARRCGVAVIASAHASDWDDAQRRPALRDILARGAFERGILLSNRLGICTCERIVHMA
                     TGTDLLDSPFLLSARADERDGGACRCGLR"
BASE COUNT          470 a          681 c          564 g          427 t
ORIGIN      
        1 gtctgcctca ggaatgcaag gaggaccgct cccaagatcg ccatgcccac aacctgaaat
       61 atctccacgt cgcagtccgc cccttaccat agtctgaata cagtgcgcac gcttgaaagc
      121 agttcctcta tataatccaa gagcatgacg aagaccacca cgagcccagc cgtggctacc
      181 agcaacgagt attcctcttt atcctgttgc ttcaggatcg tgcacagcac cgaggcgaga
      241 atgcctacgc ctgctatcct caggaccaag tcgagcccca ctgttaggcc ctccttttca
      301 ttgtgcgcaa ggttcgtcag atcaaggcta tggccaccat gccacctata aggaacccca
      361 ttgccgacca tagacggtaa ttgactgtgg cggcttcttc tgcgagctgt cttcgcctct
      421 ctagtcggcg agaggcaaga gagatatgtc gggcctgatc agcggagtcg gaggcgccca
      481 acgcctcgcc gagcactact acggcttcca gatcctcgcg ccatttgtca ctccaaagaa
      541 agctgcatga cgccgtttcc tcggtcaaag ccttcgatat gcaatcacct gccgacccgt
      601 aacccccatc ctcaagaagg tcagccgcgc gggcgaggac ccgtttgggg cttccgccag
      661 cagactgtcc cgcgtggcgc atggcttggc ccagtttggt caagccatat gatatctccg
      721 tctccagcac agcaagggca gtaacccagc cggcaagctc cctcgcgtgc gtccggtacg
      781 agcaggccac agtcagtcca gcaaggccgc tggagattat ggtaagcgcc gctccggcta
      841 tcttcagcgc aacccgcatc tgcatgcacc tccgtccctt tcgtccgccc gggccgaaag
      901 aagaaacggg gaatccaaga gatctgtccc tgtcgccatg tggacaatgc gttcacacgt
      961 gcaaattcca agcctgttgc tgaggagtat tcctctttca aacgcgcccc gagccaaaat
     1021 gtcgcgtagg gcgggcctcc tttgcgcatc atcccagtcg gaagcgtggg cgctggcaat
     1081 cacagccacg ccgcacctgc gcgcctcttc gatggcctcg ctgtcagcgg catggcctat
     1141 ctcgtcagta acgatgacat cgggcgacat cgaccttatc atgatcatca ttccttcggc
     1201 cttcgggcag ctatcgatta cgtccgctct tgggcccaag tcgtttccag gaatgccgcc
     1261 tgagcatgcg gcgatctcag aacgctcgtc cacgacgcca accatgaggc ccgttccgtt
     1321 ttgaccgtac gaaagctgtc gtgcaatatc ccggagcaga gtcgtcttgc cgtacccggg
     1381 cccggaaagg atgagagtgc tgaagactga aacccgatgg tgctgtaacg ttccgacgcg
     1441 gcgaacaatg taaggcatga tactgtctgc tgcgccgatg atctgccttg ctatccggaa
     1501 attcatggcc gaaagctccc gctgcgtccg aattcggccc ccttctagga cagcacggcc
     1561 cacgagcccc accctgtgcc ccccgcggag agtgatgtag cccgcggcga gctccctctc
     1621 aagggcgtat atggagcatt ctgttacaag ccgcatcgca gcgtcccact catcctcact
     1681 gcacatgacc gcgcttgcgg gaatctccga gctaaaggcc gccctgccac ggtccagcca
     1741 caagggttgt ccgtctgagg agaggtaaaa gtcgccctca ggcaccactc ccatggcggg
     1801 acgcccacgc ctcagcctta tctctgtaag gctctgtctg acctcatcag gcagagcctt
     1861 aacagcctcg cgggcacgtg gggggagagc cggacagatc tgttccgcca caagatctcg
     1921 aacaatgcgc ctcacaaatg tccccccaag accggtttca aatcagagac atgctattca
     1981 ataatatggg ctggcgcaag gaatatgacc ggaggaggac gccgattttc gcatgagtgc
     2041 gatagaactt gtgtgtgcat agtctgaaaa gagaattagg cagacgctct gaacgcgttg
     2101 cctgaagacg aaagcgaaag ctcacattcg accatggcat cg
//
LOCUS       DAOSYL010000140         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_39352_length_2133_cov_6.033686, whole genome shotgun sequence.
ACCESSION   DAOSYL010000140 DAOSYL010000000
VERSION     DAOSYL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39352_length_2133_cov_6.033686"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..976)
                     /gene="efp"
                     /locus_tag="PLU15_11075"
     CDS             complement(419..976)
                     /gene="efp"
                     /locus_tag="PLU15_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOO31481.1"
                     /translation="MISTNDFRTGLTIEWDGGVWTVVDFQHVKPGKGAAFVRAKLKNV
                     ITGAVIENTFRAGEKLPKAQIEYREMQFLYESGGQYFLMDQNTYDQISLSQDVLADAI
                     QYLKENMIIGVQFYEGSPIGIELPNFVELEIVETDPGLRGDTASGGTKPAKLETGAVV
                     QVPLFVQTGDKIRVDTRTNQYLERV"
     gene            complement(992..2056)
                     /locus_tag="PLU15_11080"
     CDS             complement(992..2056)
                     /locus_tag="PLU15_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOO31482.1"
                     /translation="MKMRLGKLRAQFRERDIDGFIIASYENRYYMSGFSGTAGTLVIS
                     ADDAVLITDFRYVEQAKAQAPEFRVIMHKGPMVDTVKEVVAGMGLKRVGFESARITFS
                     EYALIRDALEGVELVPEKDVVESLRMIKSDEEIALIAEACRITDAAFDHILGYIKPGV
                     TESEIANELVSFMVKHDVKPSFDFIVASGERSALPHGVASDKIIEPGDFVTLDFGGFY
                     RRYTSDMTRTVVVGTPTEEQKAIYDLVLRAQLESIDAAKAGTTGKEVDSVARGIIEHA
                     GHGEHFGHGLGHSVGLEIHENPRFSPADSSLIEPGMVLSVEPGVYVPGWGGVRIEDLV
                     VITDGEARVLSSSPKNLIQL"
BASE COUNT          482 a          660 c          477 g          514 t
ORIGIN      
        1 tctgaacgcg ttgcctgaag acgaaagcga aagctcacat tcgaccatgg catcgcctgc
       61 cttttcatta ttccagcgtg gaatcaccca atatgggcca tcttacactg catacactaa
      121 aaaagtacgg acaacgctcc taataagcca acctttcaag gccgcggccg cctcaaacac
      181 ccaagtttta caagtccaag attgaagtag gggctgaagc acgctcttgt ttttcgcgct
      241 gatcaaatca gctttgctcc gagatatgag cgataactgc acgtgccgtc ttttggggcg
      301 aatcttcgat gacatataag gaagcgaccg cgctttgcgg tcgccacatt ctacacctga
      361 ttgttttgca ccacaccaat ccaccccaaa cgcgccctgc acgtcccacc cggcgcgctt
      421 acacgcgctc cagatactgg ttggtgcggg tatctaccct aatcttgtcg cctgtctgca
      481 cgaacagggg cacttgcacc acggcgcctg tctccagctt ggcaggcttg gttccgcctg
      541 aagccgtatc gccgcgaagg ccgggatccg tctccactat ctccagctcc acgaagttcg
      601 gtagctcgat accgatgggc gagccctcgt agaactgaac tcctatgatc atgttctctt
      661 tgagatactg gatggcgtcg gccaatacgt cttggctgag ggatatctgg tcataagtat
      721 tctgatccat caggaaatac tgcccgcccg attcatacag gaactgcatc tccctgtact
      781 ctatctgtgc cttcggcagt ttctcacccg cccggaaagt attctcgatc acagcgccgg
      841 tgatcacgtt cttcagtttc gcccgaacga acgcggcgcc ctttccgggt ttgacatgct
      901 gaaaatcgac gacagtccag actccgccat cccattcgat ggtaagcccg gttctaaaat
      961 cattcgtgga aatcatgagg aacccccctg gttacagttg aatgaggttt ttcggtgaac
     1021 tgctcaggac cctagcctct ccgtcggtga ttaccacaag gtcctctatc cggactccgc
     1081 cccaaccagg cacatagacg cctggctcga cagagagcac cattcccggc tcaatcaggc
     1141 tagaatcagc aggcgagaaa cgcgggttct catgaatctc caaaccaaca gagtggccca
     1201 gcccatggcc gaaatgctcc ccgtgacccg catgttctat tattccccta gcaacgctat
     1261 ccacctcttt gccggtggtc ccagctttcg ctgcgtcgat cgactccaac tgcgccctga
     1321 gcaccaaatc gtatatggct ttctgctctt cggtgggcgt gcccacaacc acagttcggg
     1381 tcatgtcgga agtataccgc ctgtaaaacc cgccgaaatc gagtgtgacg aaatccccag
     1441 gttcgatgat cttgtcagat gccacgccat gcggcagcgc gcttctttct ccggaggcca
     1501 ctataaaatc aaagctcggc ttcacatcgt gcttgaccat aaagctgaca agctcgttgg
     1561 ctatctccga ttcggtcact cccggcttaa tgtacccgag gatatgatca aacgcggcat
     1621 ccgttatcct gcatgcctca gctatcagtg cgatctcctc atcgctcttg atcatcctga
     1681 gggactcaac aacgtccttc tcaggaacca actccacgcc ttccagagca tctcttatca
     1741 gagcatactc tgaaaacgtg atcctcgccg attcgaatcc aactcttttt aagcccatgc
     1801 cggccactac ttctttcacc gtgtccacca tcgggccttt gtgcatgatt acacggaact
     1861 cgggagcctg tgctttcgcc tgctccacgt atctgaagtc ggtgatcaat actgcgtcgt
     1921 ccgcgctgat cactaaggtc ccggcagtcc cggaaaaccc gctcatatag tagcggttct
     1981 cgtaggatgc gattataaag ccatctatat ctcgttctct aaattgggcg cgtagcttcc
     2041 cgagtctcat cttcattcct cccgcaaatt actctaccac aacattgtgg ctcaaacaat
     2101 aattggaggc aatagcgcac gctatttcac cag
//
LOCUS       DAOSYL010000141         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_40253_length_2092_cov_5.813942, whole genome shotgun sequence.
ACCESSION   DAOSYL010000141 DAOSYL010000000
VERSION     DAOSYL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40253_length_2092_cov_5.813942"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLU15_11085"
     CDS             <1..1022
                     /locus_tag="PLU15_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOO31483.1"
                     /translation="PGDPIIVDPIGPIIEDPPTPPPDDPEDPDPDDPGTGDPPTSPVT
                     EYDRLVTCYLVLNGKHLAKVVQQDSDPAEVYFCHTDMLGSIRAITDSSGEVAARFEYE
                     PFGLVATSTGPLASGAHRFTGKPEDGAIGLYYFGARYYDPEVGRFTSRDPAKDGLNWY
                     VYCINNPLNRVDFWGLHSVVYSHDEKRIYWVDDDGFIVKSWECRNAIAKGVNEKGQRR
                     APLPNGEYVATASKPGGRYGSPYGTFYIDTGDPRGRDIHGGGSGLTNPYAPRQGFVKT
                     YGCLRMQNADGEELSRLIIDHGNSVPLVVQSPEYPGPSGYGCSQGGGGGHNAYEPLEQ
                     LVSGF"
     gene            982..1680
                     /locus_tag="PLU15_11090"
     CDS             982..1680
                     /locus_tag="PLU15_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31484.1"
                     /translation="MLMNLLNNWSQDSDECGFGSRLRGPRQTMLKLLAVLGTLCALCL
                     VGLQAMAAPAVPPFEVGHPWRLTAIIVNETDPDAHPKLWIASAEVSLPKDSSAVLQLY
                     FSPPGVGLCEPVTLLGAEVVVEYCDIWGKALARGSAKVKAPETDEKPVAASPIRLKGI
                     PTDTDYIFVWLKWMNISGELIEFNWPADPHQLGVAVLRERLRLADYLGYEPNDDVQAL
                     IDRLVREISDAFRE"
BASE COUNT          477 a          513 c          635 g          467 t
ORIGIN      
        1 tcccgggcga cccgataata gtcgatccga ttggtccgat aattgaggat ccgccgactc
       61 caccgcctga tgacccagag gatcctgacc cagatgatcc cggcacgggc gatccgccca
      121 cttccccggt gacggagtat gacagactcg tcacatgcta ccttgtcctg aacgggaaac
      181 acttggctaa ggtagtacag caagactcgg atcctgctga agtctacttt tgccacaccg
      241 acatgctagg atccataagg gcgatcaccg actcttcggg cgaggtcgca gcgaggttcg
      301 agtatgagcc gttcgggttg gttgccacat caacagggcc gctggccagc ggagcgcata
      361 gattcactgg aaagcctgag gatggcgcta tagggctcta ctactttggg gccaggtact
      421 atgaccccga ggtgggcagg tttacgagta gggatccagc gaaagatggg ctgaactggt
      481 acgtatactg cattaacaac ccgctgaata gagtagactt ctgggggctg cactcagtgg
      541 tgtatagcca cgatgagaaa agaatttact gggttgacga tgatgggttc atagtgaaat
      601 cgtgggagtg cagaaatgcc atcgccaaag gcgtgaatga gaagggacag cgaagggcgc
      661 cattgcccaa tggtgagtat gtcgccacag cctccaagcc gggcggtaga tacggcagtc
      721 cctatggaac attctacata gatactggtg atccgagagg tcgagatatt cacggaggcg
      781 ggagtgggtt aaccaatcca tacgctccgc gtcagggctt tgtgaagacg tatgggtgct
      841 tgcgtatgca gaatgccgat ggcgaagagc tgagcagact cattatagat catgggaact
      901 ccgttccgct ggtggtgcag tcgcctgagt accccggccc ttccggatac gggtgttccc
      961 aagggggagg aggaggtcac aatgcttatg aacctcttga acaattggtc tcaggattct
     1021 gatgaatgtg ggttcgggtc gaggttgcgc ggccccagac agacgatgct caagctgctg
     1081 gctgttttgg gcacactctg tgctctctgc ctggtcggtc tgcaagcgat ggcagcgccg
     1141 gctgttccgc cttttgaggt tgggcatccc tggcgcctta cagcaatcat tgtcaacgaa
     1201 acggaccccg atgctcatcc gaagctatgg atagcctccg cagaggtgtc gcttccaaag
     1261 gacagcagcg ctgtgcttca actgtacttc tcaccaccag gagtagggct gtgtgaacca
     1321 gtgacgcttc tcggagccga ggtcgtagtt gaatactgtg acatttgggg caaggccctt
     1381 gctcgaggat cagccaaagt aaaagcgcct gagaccgacg aaaagccagt cgctgccagc
     1441 cccatccgcc tgaagggcat acccacggat actgactaca tattcgtatg gctgaaatgg
     1501 atgaacatct ccggcgagct tatcgaattc aactggccgg cagacccgca tcagcttggc
     1561 gtggctgtgc tgagggaacg attgcggcta gctgattacc ttggttatga gcccaatgac
     1621 gatgtgcaag cgctcataga taggcttgtg agggaaatat ctgacgcatt tcgcgaatag
     1681 tggtgtctcg catggatgaa gcgccggtct ggatcaatcc tgcatgagac agatacgctc
     1741 atgtatgcaa gtagtgaatc gtgagctaat ggaaccaggg aacccttgcc cccaaccgcc
     1801 gccagcgggg atgcatcctg taggcaagga cgtttctcgc cgcgtcgcgt tccacggtct
     1861 atgcctgtga ttacacggga ggaatctcag tagagatggt tcgctttcct atgggtgcct
     1921 gtcttataag ccgcgcaggt ctcttggcga gatgtatgag cggctatttt caggattgcc
     1981 taaaggctgg gttgtgtatg attgatcaga cagatgacat gtggagagtc aggaggcgac
     2041 ctgcacatga agataggctt gaggaacggt ttgtgggtca ttttgggtgt tg
//
LOCUS       DAOSYL010000142         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_45965_length_1885_cov_8.588525, whole genome shotgun sequence.
ACCESSION   DAOSYL010000142 DAOSYL010000000
VERSION     DAOSYL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45965_length_1885_cov_8.588525"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1883
                     /locus_tag="PLU15_11095"
     CDS             186..1883
                     /locus_tag="PLU15_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO31485.1"
                     /translation="MTQINSVAARVFGVVLAIVIIPVIAVSGASTTSEVAPGTSAVAP
                     SRAEVEAFYSYDAGIPLDVKEIPLGSSPWYREYEISYANLGDTVYSRFMLPREAGASF
                     ETMNPMPCLVGLHGMFSDSAYQFWTIADFCAKRKVAVMTPSLPYHHKRTKGMPLIPGQ
                     QLIVGSPDAIRNNLRRAVIDVRRAVDWLATRDDIDQGAISIAGVSLGGIVASLAFKVE
                     PRFANAVLVVAGSGVRGILENGDTSVLDVFRAATWANMVNPDDFVDALQIVDPINVPD
                     FQPRPALLMNGISDAIMVPDNVTALRRSMARAEQIWTTGGHYFPMYPAEYLLMDYLSR
                     QYAESPSFAQGIRVDVGSGFCFTERVCAPEVGADNLWVDIAVELGSAEDVAAHRVSVP
                     MVNRSVPVIILSRETYLALEPFLMGRHLPAFIYIINGDSPLQLCAAVAYVQALGLGAD
                     PQLYYVSPLEDSENNPGQEGYAVCAASPMDVGRVRNMLESIARAPGSRTSGGFEKANA
                     RIPLTLFGEAKRFQGPTAREDIGLWFAKTLSVSKLKGIPWFPNYPHDDPCWSAGPDR"
BASE COUNT          380 a          495 c          548 g          462 t
ORIGIN      
        1 ttacgggata tgtgaagcgg aaccccgagc ttgaccatct gttctatata ggtgttctgc
       61 tgtcttgtta ctttagcccg gccgggcgac tcgcggtcgt ggacaattgg gaaatcaaca
      121 aggagtggaa tgccgcggcc tgctggtgta tgatgtataa aacttgccag gaggttctta
      181 cggttatgac acaaataaac tcagtagcag ccagagtctt cggagttgtc ctcgctatcg
      241 tgataattcc ggtcatagct gtatcgggag cttctacgac ttcagaagtc gctcccggga
      301 cgtctgccgt ggctccatcc cgagccgagg tggaggcctt ttattcctat gatgcaggca
      361 tcccccttga tgtcaaagag atcccgttgg gatctagtcc ctggtatcgg gagtatgaga
      421 tcagctatgc taaccttgga gacaccgtgt attccaggtt catgcttcca cgggaggcgg
      481 gggcgtcttt cgaaacgatg aacccgatgc cctgcctagt cggcctccat ggcatgttct
      541 ctgactcagc gtaccagttt tggaccattg cggacttctg tgccaagcga aaagttgcag
      601 taatgacccc cagccttcca taccaccaca agcgcacgaa ggggatgcct ctcataccgg
      661 gacagcagct aatcgttggt tcgcctgatg cgatcagaaa caatctgcgt cgagcggtca
      721 tagatgtccg tcgcgctgta gattggcttg ccacgcgtga cgatatcgac cagggcgcta
      781 tatcgattgc aggcgtcagt ctgggcggaa ttgttgcctc gttggccttc aaggttgagc
      841 caaggttcgc aaatgccgtg ctggtagtgg ctggctcagg ggttagaggc atacttgaga
      901 acggggatac gagtgtcctc gatgtcttcc gcgcagccac ctgggcaaac atggtaaacc
      961 ccgatgattt cgtcgatgcg cttcagatcg tcgatcccat caatgtgccg gatttccagc
     1021 cccgcccggc gcttctaatg aacggaattt ccgatgcgat catggtcccc gataacgtga
     1081 cggcgcttcg ccgttccatg gctcgagctg agcagatatg gaccacgggc gggcactatt
     1141 ttccgatgta cccagcggaa tacttgctca tggactattt gtctaggcag tatgcggaat
     1201 ctccgtcatt tgctcaaggc attcgtgtgg atgtaggctc tggattctgt ttcaccgaaa
     1261 gggtgtgcgc accggaagtg ggggccgaca acttgtgggt agacatcgca gttgagcttg
     1321 gcagtgccga ggacgtcgct gctcaccggg tttcggtgcc gatggttaac aggtcggttc
     1381 cggtcataat cctgagcagg gagacttatt tggctctcga gcctttcttg atgggacggc
     1441 atcttcctgc gttcatctat atcatcaacg gtgattctcc actacagctg tgcgccgccg
     1501 tggcatatgt acaggcgttg gggcttggcg ccgacccgca gctctattac gtctcccctc
     1561 tggaggattc agaaaacaac ccgggccagg aaggatatgc cgtctgtgct gcatctccca
     1621 tggatgtggg gagggtccgc aatatgttag agtcgatcgc acgagctcct ggttctagga
     1681 cctcgggggg ctttgagaag gcgaatgccc gaatccccct gacgctcttt ggcgaagcaa
     1741 agcgttttca aggcccgact gctcgtgagg acatcgggtt gtggtttgcc aagacgctct
     1801 cggtgagcaa actcaagggc atcccttggt tcccgaatta tccccatgat gacccatgtt
     1861 ggtcggcggg gcccgacaga tgact
//
LOCUS       DAOSYL010000143         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_48641_length_1806_cov_5.663621, whole genome shotgun sequence.
ACCESSION   DAOSYL010000143 DAOSYL010000000
VERSION     DAOSYL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48641_length_1806_cov_5.663621"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..430)
                     /locus_tag="PLU15_11100"
     CDS             complement(161..430)
                     /locus_tag="PLU15_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31486.1"
                     /translation="MQTMGRENTVIGCMIGGNVRDMLRQACGELKILLYETDCSTDLI
                     AIPSMLSVVDPSAMEPRDLADVLDWVTDLEDPQYRILFTEAVPGL"
     gene            618..800
                     /locus_tag="PLU15_11105"
     CDS             618..800
                     /locus_tag="PLU15_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOO31487.1"
                     /translation="MSSREVIKLIEQDGWYLVAVAGSHHQFKHPHKTGKVTIPHPSRT
                     LAPKTVKSILRQAGLP"
     gene            833..1252
                     /locus_tag="PLU15_11110"
     CDS             833..1252
                     /locus_tag="PLU15_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO31488.1"
                     /translation="MARYIYPAIFDPNELGGYTITFPDLPGCVTQGDTIDNALHMAAE
                     AMALHLYGMERDSDEIPVPSEPAHIPFPEDASDNAFVTLVAASTEPVRDEIRNRAVKK
                     TLTIPAWLNEESEKAGINFSQTLQSALKERLGLIDRP"
     gene            complement(1303..1539)
                     /locus_tag="PLU15_11115"
     CDS             complement(1303..1539)
                     /locus_tag="PLU15_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO31489.1"
                     /translation="MTQVETDGEVFATILVGAEEGDVQTVLVKAASAGEAISAVLHDS
                     RWGSYACPVAFTVDEVESILDSMRAGIVELCAMA"
BASE COUNT          433 a          529 c          471 g          373 t
ORIGIN      
        1 gaaaggaaca tcccggaaga aggactgcgt attgtaaaca cggtttggca tcggtttgta
       61 aggcaggaac tgccggaagt tacgcctgtg gagaccgtgt aagaccagca atggcaacgg
      121 tccgcgaagc aggaatcccg cgacttcagt cgtgggaggt tcaaagtcca ggaactgcct
      181 cggtgaagag gattctgtac tggggatcct cgagatcggt tacccaatcc agcacgtccg
      241 caagatccct gggttccatt gcagatggat ctaccacaga gagcatagac ggtattgcta
      301 tcaggtcagt agagcagtca gtctcatata gcaggatctt tagctcgccg caagcctgtc
      361 tcagcatgtc cctcacattc ccgccgatca tgcaaccaat cacagtattc tctctaccca
      421 tggtctgcat atcaatcacc ccaattcgat gctagcacac gtcggcgaca gcgtattgac
      481 gcgcagttag ggcgcctcag gatgtcttcc cgcacctggc gatgcgcctc ttcaaccacg
      541 tcttgaccgt aacactgttg acacgtatta ttagcacgtg ttaggattcg attaggggga
      601 cgatagcagt gacgtgcatg tcttcccgcg aggttatcaa gctaatagag caggatgggt
      661 ggtacctggt ggcagtagcc ggcagccacc accagttcaa acatccacat aagacaggta
      721 aggtcaccat ccctcatccg agcaggaccc ttgctccgaa gacggtcaag tcaatactca
      781 ggcaggcagg gctgccctag gcaaaccgac ctcatagagg aggcctggat tcatggccag
      841 atacatctat ccggcgatct tcgatcccaa tgaactcggg ggatacacca tcacattccc
      901 cgatctgccc gggtgcgtta cccaggggga taccattgat aatgcgcttc atatggccgc
      961 agaagccatg gctctacacc tgtacggcat ggagcgagac agcgacgaga tcccagtccc
     1021 gtcggagccc gcgcatatcc ctttcccaga ggatgcaagc gacaacgcct tcgtcactct
     1081 cgttgccgcc agcaccgagc cggtgagaga cgagatacga aaccgagccg tgaagaagac
     1141 gctcaccatc cccgcatggc tcaacgaaga atccgagaaa gcggggatca acttctcaca
     1201 gaccctccag tcggcgctca aagagaggct cggactgatt gaccggccgt gaccacgggg
     1261 cctcaccggg ggggcagctt cctcatgcat gaatccttgt gttcatgcca tggcacacaa
     1321 ttccactatg ccggccctca tagaatccag gatggattcc acttcatcga ccgtgaaagc
     1381 gaccgggcat gcgtagctcc cccatctcga atcgtgcagt actgctgaaa tggcctctcc
     1441 ggcagaggct gccttcacca aaacggtctg tacatcacct tcctcagcgc cgacaaggat
     1501 cgtggcaaat acctcgccgt ctgtctccac ctgcgtcatt tcgacttgct cacctcctgt
     1561 tgctttttct atctattgtg tagtccagtc gcggaaaagg tccttccacc tttcttggag
     1621 actccgttgg atcgcccacg accagagtag gacttgcatc tggacctatg aggtggtgaa
     1681 tacctgtaag gcaccgcatt gtccatcgac agaagaagct gtcggcaccg aaagacagga
     1741 gcgacacgca cgaatgacgt ctgctgcaag cgcgtcgacg agcactgatc atccgaagtt
     1801 gcatgg
//
LOCUS       DAOSYL010000144         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_2086
            NODE_49371_length_1785_cov_11.202890, whole genome shotgun
            sequence.
ACCESSION   DAOSYL010000144 DAOSYL010000000
VERSION     DAOSYL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864153
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:25:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,179
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 19 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49371_length_1785_cov_11.202890"
                     /isolate="AMR_MDS_2086"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..>1785
                     /locus_tag="PLU15_11120"
     CDS             95..>1785
                     /locus_tag="PLU15_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOO31490.1"
                     /translation="MTEYTEFLQSKRLIVPSSGLKVSAEAIHPKLFDWQKDIVCWALR
                     KGRAAIFADCGLGKTFMQLEWARLIGGRALIVAPLSVARQTVGEAAKIDMAVHYTRSG
                     DDLIDGINITNYEMVERFDASDFASVVLDESSILKGLDGKTRRRVTEMFAETPYRLCC
                     TATPAPNDIAEIANHAEFLGIMTRAEMLAAFFVHDDKGWRLKGHAREGFFRWLASWGM
                     SIRKPSDLGYSDEGFILPDLDIRADFVRAENAPEGQLFWGALHGLSDRIAARRATVRE
                     RAERAAEIINSNGEQWIAWVGLNDEGRMLAQLVPDSALQEGADSPDKKLADIEAFQDG
                     RVRVLITKPRIAGFGMNLQNCSNMVFVGLGDSYEQYYQCIRRCWRFGQSRPVTAHIVL
                     TDMETEIHENVLRKEAQAAEMAEELLANVIEFEREEIAETQANFEYAEDTVRGDGYVM
                     MLGDSAEKLREIDDESIGLSVFSPPFISLYTYSPTERDLGNCRTEDEFFRHFGFIIDE
                     LLRVTMPGRNCCVHVAQVPAMLSRDGYIGLKDFRGQTIQTFEQHGWIYHGEVCID"
BASE COUNT          411 a          419 c          606 g          349 t
ORIGIN      
        1 cgggactggc gggccgggaa gatcggcaat caggagttaa tctggataac atggcaggcg
       61 cagcagaggt atggcgtagg ggaggtgttc ggcagtgacg gaatacacgg agtttctgca
      121 atcgaagcgg ctgatagtcc cctcatcagg gctaaaggta tcggcagagg cgatacaccc
      181 gaagctattt gactggcaga aggacattgt gtgctgggca ctgcgcaagg gccgggcggc
      241 catattcgcg gattgcgggc tcggcaaaac gttcatgcaa cttgaatggg cacggctcat
      301 cggcggaagg gctttgatag tcgcgccgtt gtcggtagcg cggcagacgg tgggcgaagc
      361 cgccaagatc gatatggcag ttcattacac acgctccggc gatgacttga tagacggcat
      421 taacataaca aactacgaga tggtggagcg attcgatgca agcgacttcg cctccgtggt
      481 gctggatgaa agcagcatcc tgaaaggact ggacggcaag acgcggcggc gagtgacgga
      541 gatgttcgcc gagactccat acaggttgtg ctgcacggcg actcctgcgc ctaacgatat
      601 cgcggagata gcgaatcatg cggagttcct gggcattatg actcgcgctg aaatgttggc
      661 tgcgttcttt gtgcatgatg acaagggctg gagactcaaa ggccatgccc gcgaaggatt
      721 cttccgctgg ctcgccagct ggggcatgtc gattcgcaag ccttcggacc tggggtattc
      781 ggacgaagga ttcatcctgc cggacctgga tattcgcgcg gacttcgtca gagcggaaaa
      841 cgcgcccgaa ggccagttgt tttggggtgc tctacacggc ctgagcgaca ggatagcggc
      901 tcgacgggcc acggtgcgcg aacgcgcgga aagagcagcg gagatcatca acagcaatgg
      961 cgagcagtgg atagcgtggg ttgggttgaa tgatgagggg cgaatgttgg cgcagctggt
     1021 gccggattcg gcattgcagg aaggcgcgga tagtcccgac aagaagctgg ccgacatcga
     1081 agcctttcag gatggacgcg tgagggtgct tattaccaag ccgcgaatag cgggattcgg
     1141 catgaatctt caaaactgct cgaacatggt gttcgtcggg ctaggagatt cctatgaaca
     1201 gtattaccag tgcattagga ggtgttggag attcgggcag tcgcgcccgg tgacggccca
     1261 tatcgtgttg acggacatgg aaacggagat ccacgagaac gtgttacgga aggaggcgca
     1321 ggccgcggag atggctgagg agttgttggc gaacgtgata gagttcgagc gcgaagagat
     1381 agccgaaaca caggccaatt tcgagtatgc cgaggacacg gtgcgcggag acggatacgt
     1441 gatgatgctg ggcgatagcg ccgagaagct gcgcgagata gacgatgaga gtatcgggtt
     1501 gtctgtgttc tcgccgccgt ttatcagcct atacacctac agtcccacag agcgcgatct
     1561 gggcaattgc aggacagaag atgagttctt caggcacttc ggattcatca tcgacgaact
     1621 tctgcgggtg actatgccgg ggcgcaactg ctgcgtgcac gtcgcccagg tgcccgcgat
     1681 gctatcccga gacgggtata tcggtctgaa ggacttccgc gggcagacga ttcagacgtt
     1741 tgaacagcat ggatggatat atcacgggga ggtgtgcatc gacaa
//
